LOCUS       DAVHEX010000001        76339 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVHEX010000001 DAVHEX010000000
VERSION     DAVHEX010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 76339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76339
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.001"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..429
                     /locus_tag="VVL45_000001"
     CDS             <1..429
                     /locus_tag="VVL45_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4186265.1"
                     /translation="LTIAGLGGILGNMLAPIVIKYLKSNQIVGVFLFLNGSSWLVAIV
                     IKDYTLSLILFFVCFMSKGVFNIIFNSLYQQIPPHQLLGRVNTTIDSIISFGMPIGSL
                     VAGTLIDLNIELVLIAISIPYFLFSYIFYTDNGLKEFSIY"
     gene            854..1087
                     /locus_tag="VVL45_000002"
     CDS             854..1087
                     /locus_tag="VVL45_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU4186266.1"
                     /translation="MISIDLGGTNIKITVLSNDGEIETLWSITTDTSEKGSQIISDII
                     SSIKNKLTERNIPDSDLLGIGMGSCSSYFPCKS"
     gene            1370..3319
                     /gene="mutL"
                     /locus_tag="VVL45_000003"
     CDS             1370..3319
                     /gene="mutL"
                     /locus_tag="VVL45_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEU4186267.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(3644..4111)
                     /locus_tag="VVL45_000004"
     CDS             complement(3644..4111)
                     /locus_tag="VVL45_000004"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEU4186268.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(4112..5317)
                     /locus_tag="VVL45_000005"
     CDS             complement(4112..5317)
                     /locus_tag="VVL45_000005"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEU4186269.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAKMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(5337..5972)
                     /gene="ribE"
                     /locus_tag="VVL45_000006"
     CDS             complement(5337..5972)
                     /gene="ribE"
                     /locus_tag="VVL45_000006"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEU4186270.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLEQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(5957..7057)
                     /gene="ribD"
                     /locus_tag="VVL45_000007"
     CDS             complement(5957..7057)
                     /gene="ribD"
                     /locus_tag="VVL45_000007"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEU4186271.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHHVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKVFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(7191..7305)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            7462..8055
                     /gene="ruvA"
                     /locus_tag="VVL45_000008"
     CDS             7462..8055
                     /gene="ruvA"
                     /locus_tag="VVL45_000008"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEU4186272.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEA
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDDLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            8065..8628
                     /locus_tag="VVL45_000009"
     CDS             8065..8628
                     /locus_tag="VVL45_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEU4186273.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQVLFELLCMETYQA
                     GLSWETVLNKRQAFREVFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKLFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            8625..9302
                     /locus_tag="VVL45_000010"
     CDS             8625..9302
                     /locus_tag="VVL45_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU4186274.1"
                     /translation="MMSNKNKEILIFAILYTVLFMFDGVKLLASLMPSAIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVGL
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLSGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(9439..10470)
                     /locus_tag="VVL45_000011"
     CDS             complement(9439..10470)
                     /locus_tag="VVL45_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEU4186275.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVILFDYNK"
     gene            complement(10725..11408)
                     /locus_tag="VVL45_000012"
     CDS             complement(10725..11408)
                     /locus_tag="VVL45_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEU4186276.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILTSMFLWLLVFFATSFLPASLNPVLDPLPLAIIGVALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(11420..12364)
                     /locus_tag="VVL45_000013"
     CDS             complement(11420..12364)
                     /locus_tag="VVL45_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU4186277.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            12496..15327
                     /gene="uvrA"
                     /locus_tag="VVL45_000014"
     CDS             12496..15327
                     /gene="uvrA"
                     /locus_tag="VVL45_000014"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEU4186278.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLHEGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVTGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDAGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            15320..16381
                     /locus_tag="VVL45_000015"
     CDS             15320..16381
                     /locus_tag="VVL45_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEU4186279.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMNLTRIGFEDEISVSYY
                     HRMQAAFAGLNLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            16513..16911
                     /gene="spx"
                     /locus_tag="VVL45_000016"
     CDS             16513..16911
                     /gene="spx"
                     /locus_tag="VVL45_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEU4186280.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            16977..17546
                     /locus_tag="VVL45_000017"
     CDS             16977..17546
                     /locus_tag="VVL45_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU4186281.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLAIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            17633..17899
                     /locus_tag="VVL45_000018"
     CDS             17633..17899
                     /locus_tag="VVL45_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEU4186282.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            17903..18322
                     /gene="ruvX"
                     /locus_tag="VVL45_000019"
     CDS             17903..18322
                     /gene="ruvX"
                     /locus_tag="VVL45_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEU4186283.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            18338..18643
                     /locus_tag="VVL45_000020"
     CDS             18338..18643
                     /locus_tag="VVL45_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEU4186284.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            18902..20152
                     /locus_tag="VVL45_000021"
     CDS             18902..20152
                     /locus_tag="VVL45_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEU4186285.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLTDQVKE
                     MEKTLLQTPAQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGEFAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLACQKKAESLAVNLYQA
                     GQDFLMLNGDFSSSLLNLSQLNIGLEGVYQQENAALALQTFLLFMRERKEAVDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLAEFIKEKEQEGYRPQILFGSLKRKD
                     YQGMLGYLTEKLPQVELKVTGFDYQGALDERDVTGYDIVSSYREFISDFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            20149..20694
                     /locus_tag="VVL45_000022"
     CDS             20149..20694
                     /locus_tag="VVL45_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HEU4186286.1"
                     /translation="MTPFFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQQVQQPAQQNTNTANAGGNQNQAAPVQNQPVAQPTDID
                     GTYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTLDSANQRMIIGDDVKIYTVN
                     GNQIVVDDMDRDPSDQIVLTK"
     gene            20840..22372
                     /gene="cls"
                     /locus_tag="VVL45_000023"
     CDS             20840..22372
                     /gene="cls"
                     /locus_tag="VVL45_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEU4186287.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            22630..24168
                     /locus_tag="VVL45_000024"
     CDS             22630..24168
                     /locus_tag="VVL45_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEU4186288.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            24288..26495
                     /gene="nrdD"
                     /locus_tag="VVL45_000025"
     CDS             24288..26495
                     /gene="nrdD"
                     /locus_tag="VVL45_000025"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEU4186289.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKCLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKQEDYAWK
                     KAQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            26507..26647
                     /locus_tag="VVL45_000026"
     CDS             26507..26647
                     /locus_tag="VVL45_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4186290.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            26691..27197
                     /locus_tag="VVL45_000027"
     CDS             26691..27197
                     /locus_tag="VVL45_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4186291.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKFQSPHDGGFWDTENFVYEDWL
                     ESNQEQEMGINLPEGWVPAIQLVAFSEKGQAVGFLNLRLRLSNFLLEEGGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDARNGVER
                     YWIEVANE"
     gene            27190..27780
                     /gene="nrdG"
                     /locus_tag="VVL45_000028"
     CDS             27190..27780
                     /gene="nrdG"
                     /locus_tag="VVL45_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEU4186292.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLEFLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            27777..28394
                     /locus_tag="VVL45_000029"
     CDS             27777..28394
                     /locus_tag="VVL45_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEU4186293.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRHLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            28661..28969
                     /gene="rpsJ"
                     /locus_tag="VVL45_000030"
     CDS             28661..28969
                     /gene="rpsJ"
                     /locus_tag="VVL45_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEU4186294.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            29186..29812
                     /gene="rplC"
                     /locus_tag="VVL45_000031"
     CDS             29186..29812
                     /gene="rplC"
                     /locus_tag="VVL45_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEU4186295.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            29837..30460
                     /gene="rplD"
                     /locus_tag="VVL45_000032"
     CDS             29837..30460
                     /gene="rplD"
                     /locus_tag="VVL45_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEU4186296.1"
                     /translation="MANVTLFDQTGKEAGQVVLSDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            30460..30756
                     /locus_tag="VVL45_000033"
     CDS             30460..30756
                     /locus_tag="VVL45_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEU4186297.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            30774..31607
                     /gene="rplB"
                     /locus_tag="VVL45_000034"
     CDS             30774..31607
                     /gene="rplB"
                     /locus_tag="VVL45_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEU4186298.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            31711..31992
                     /gene="rpsS"
                     /locus_tag="VVL45_000035"
     CDS             31711..31992
                     /gene="rpsS"
                     /locus_tag="VVL45_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEU4186299.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            32004..32348
                     /gene="rplV"
                     /locus_tag="VVL45_000036"
     CDS             32004..32348
                     /gene="rplV"
                     /locus_tag="VVL45_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEU4186300.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIATLTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            32361..33014
                     /gene="rpsC"
                     /locus_tag="VVL45_000037"
     CDS             32361..33014
                     /gene="rpsC"
                     /locus_tag="VVL45_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEU4186301.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            33018..33431
                     /gene="rplP"
                     /locus_tag="VVL45_000038"
     CDS             33018..33431
                     /gene="rplP"
                     /locus_tag="VVL45_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEU4186302.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            33441..33647
                     /locus_tag="VVL45_000039"
     CDS             33441..33647
                     /locus_tag="VVL45_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEU4186303.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            33672..33932
                     /gene="rpsQ"
                     /locus_tag="VVL45_000040"
     CDS             33672..33932
                     /gene="rpsQ"
                     /locus_tag="VVL45_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEU4186304.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            33958..34326
                     /gene="rplN"
                     /locus_tag="VVL45_000041"
     CDS             33958..34326
                     /gene="rplN"
                     /locus_tag="VVL45_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEU4186305.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            34404..34709
                     /gene="rplX"
                     /locus_tag="VVL45_000042"
     CDS             34404..34709
                     /gene="rplX"
                     /locus_tag="VVL45_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEU4186306.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            34733..35275
                     /gene="rplE"
                     /locus_tag="VVL45_000043"
     CDS             34733..35275
                     /gene="rplE"
                     /locus_tag="VVL45_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEU4186307.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            35293..35562
                     /gene="rpsN"
                     /locus_tag="VVL45_000044"
     CDS             35293..35562
                     /gene="rpsN"
                     /locus_tag="VVL45_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEU4186308.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            35847..36245
                     /gene="rpsH"
                     /locus_tag="VVL45_000045"
     CDS             35847..36245
                     /gene="rpsH"
                     /locus_tag="VVL45_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEU4186309.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            36437..36973
                     /gene="rplF"
                     /locus_tag="VVL45_000046"
     CDS             36437..36973
                     /gene="rplF"
                     /locus_tag="VVL45_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEU4186310.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGSKGELTREFSKDIEIR
                     VEGTEITLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            37057..37413
                     /gene="rplR"
                     /locus_tag="VVL45_000047"
     CDS             37057..37413
                     /gene="rplR"
                     /locus_tag="VVL45_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEU4186311.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            37431..37925
                     /gene="rpsE"
                     /locus_tag="VVL45_000048"
     CDS             37431..37925
                     /gene="rpsE"
                     /locus_tag="VVL45_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEU4186312.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            37897..38121
                     /gene="rpmD"
                     /locus_tag="VVL45_000049"
     CDS             37897..38121
                     /gene="rpmD"
                     /locus_tag="VVL45_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEU4186313.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            38266..38706
                     /gene="rplO"
                     /locus_tag="VVL45_000050"
     CDS             38266..38706
                     /gene="rplO"
                     /locus_tag="VVL45_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEU4186314.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            38719..40029
                     /gene="secY"
                     /locus_tag="VVL45_000051"
     CDS             38719..40029
                     /gene="secY"
                     /locus_tag="VVL45_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEU4186315.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            40180..40818
                     /locus_tag="VVL45_000052"
     CDS             40180..40818
                     /locus_tag="VVL45_000052"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEU4186316.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGVINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            40935..41153
                     /gene="infA"
                     /locus_tag="VVL45_000053"
     CDS             40935..41153
                     /gene="infA"
                     /locus_tag="VVL45_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEU4186317.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            41178..41294
                     /gene="rpmJ"
                     /locus_tag="VVL45_000054"
     CDS             41178..41294
                     /gene="rpmJ"
                     /locus_tag="VVL45_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEU4186318.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            41312..41677
                     /gene="rpsM"
                     /locus_tag="VVL45_000055"
     CDS             41312..41677
                     /gene="rpsM"
                     /locus_tag="VVL45_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEU4186319.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            41695..42078
                     /gene="rpsK"
                     /locus_tag="VVL45_000056"
     CDS             41695..42078
                     /gene="rpsK"
                     /locus_tag="VVL45_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEU4186320.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            42121..43056
                     /locus_tag="VVL45_000057"
     CDS             42121..43056
                     /locus_tag="VVL45_000057"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEU4186321.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            43068..43454
                     /gene="rplQ"
                     /locus_tag="VVL45_000058"
     CDS             43068..43454
                     /gene="rplQ"
                     /locus_tag="VVL45_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEU4186322.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTESRRGDAAPMAIIELV"
     gene            43719..43985
                     /locus_tag="VVL45_000059"
     CDS             43719..43985
                     /locus_tag="VVL45_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEU4186323.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            43995..45332
                     /locus_tag="VVL45_000060"
     CDS             43995..45332
                     /locus_tag="VVL45_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEU4186324.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPDINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGKEGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
     gene            45576..46268
                     /locus_tag="VVL45_000061"
     CDS             45576..46268
                     /locus_tag="VVL45_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU4186325.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGEIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEMIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
     gene            complement(46371..48016)
                     /locus_tag="VVL45_000062"
                     /pseudo
     CDS             complement(46371..48016)
                     /locus_tag="VVL45_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(48006..49097)
                     /locus_tag="VVL45_000063"
     CDS             complement(48006..49097)
                     /locus_tag="VVL45_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4186326.1"
                     /translation="MSEIKIINAKKIYHDVPVIENLNITIPKGSLFTLLGASGCGKTT
                     LLRMIAGFNSIEGGEFYFDDTKINNMEPSKRNIGMVFQNYAIFPHLTVRDNVAFGLMQ
                     KKVPKEELIQQTNKYLELMQIAQYADRKPDKLSGGQQQRVTLACALAVNPSVLLMDEP
                     LSNLEAKLRLDMRQAIREIQHEVGITTVYVTHDQEEAMAISDQIAVMKDGVIQQIGRP
                     KELYHKPANEFVATFIGRTNIIPANLEKRSDGAYIVFSDGYALRMPALDQVEEQAIHV
                     SIRPEEFIKDESGDIEGTIRDSVYLGLNTDYFIETGFASKIQVSEESTFEEDLQKGNR
                     IRLRINTQKLNIFSADGSQNLIKGVNHGT"
     gene            complement(49110..50126)
                     /locus_tag="VVL45_000064"
     CDS             complement(49110..50126)
                     /locus_tag="VVL45_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU4186327.1"
                     /translation="MKKKWMYYAACSSNESADDSSSDKGDGGSLVVYSPNSEGLIGAT
                     IPAFEEKYGIKVELIQAGTGELFKKLESEKEVPVADVIFGGSYTQYTTHGELFENYTS
                     KENDNVIKEYQNTTGYSTPYTLDGSVLIVNPDLTKGMNIEGYNDLFKPELKGKIATAD
                     PANSSSAFAQLTNMLQAQGGYKDDKAWSYVKDLFTLIDGKIGSSSSSVYKVVADGEMA
                     VGLSYEDPAVKLLNDGANIKVVYPKEGTVFLPASAAIVKKSKNMENAKKFIDFIISQE
                     VQDTLGTTTTNRPVRKNAKTSENMKPIDKIKTLTEDYDYVIKNKSDIVKKYNEVFTDI
                     QSKQ"
     gene            complement(50713..51489)
                     /locus_tag="VVL45_000065"
     CDS             complement(50713..51489)
                     /locus_tag="VVL45_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEU4186328.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVTGVFN
                     QMIIKNIHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFG
                     ENKYRLLNRKYEMDGINALHPEDLIDYQKVFLNHHINCYF"
     gene            51612..52358
                     /locus_tag="VVL45_000066"
     CDS             51612..52358
                     /locus_tag="VVL45_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU4186329.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYETKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRVIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     CQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAVSDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            52371..53351
                     /locus_tag="VVL45_000067"
     CDS             52371..53351
                     /locus_tag="VVL45_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEU4186330.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNVGIVKIEIENFDTNMFKLENYVKEKYSLESLEIIPNEFDDTPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
     gene            53546..53866
                     /locus_tag="VVL45_000068"
     CDS             53546..53866
                     /locus_tag="VVL45_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU4186331.1"
                     /translation="MEMIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            53912..54220
                     /locus_tag="VVL45_000069"
     CDS             53912..54220
                     /locus_tag="VVL45_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU4186332.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQSSGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            54237..55535
                     /locus_tag="VVL45_000070"
     CDS             54237..55535
                     /locus_tag="VVL45_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU4186333.1"
                     /translation="MSKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASANLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVITQLVILAMSTLVYFPFFKVQDRLAYQNEIKQS"
     gene            55686..58124
                     /locus_tag="VVL45_000071"
     CDS             55686..58124
                     /locus_tag="VVL45_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEU4186334.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMTIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNLLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            58143..58811
                     /locus_tag="VVL45_000072"
     CDS             58143..58811
                     /locus_tag="VVL45_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEU4186335.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHKIRRQAGDDIFIKVPVTPAGLRAIKALK
                     KEGYHITATAIYTVIQGLLAIEAGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVIQN
                     SRSI"
     gene            58829..59917
                     /locus_tag="VVL45_000073"
     CDS             58829..59917
                     /locus_tag="VVL45_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEU4186336.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNCPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            60372..62873
                     /locus_tag="VVL45_000074"
     CDS             60372..62873
                     /locus_tag="VVL45_000074"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEU4186337.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFIVLAPEHELVDAITSSEQ
                     AEAVADYKHQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEMPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVAWLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALD
                     KAYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEISLADEKVKAEIDGKEIVKVIAVPNKLVNIVVK"
     gene            63067..63762
                     /locus_tag="VVL45_000075"
     CDS             63067..63762
                     /locus_tag="VVL45_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4186338.1"
                     /translation="MPVNEYGQMIGESMEAYTPGELPSFDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPREMWTYLFQESVADMEELVSLLNQMLARKDRFYYAIIDKATGKALG
                     TFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLACYVFEELNYRRYEWKCDAL
                     NLPSRRAAERLGFIYEGTFRQAVVYKGRTRDTDWLSMIDKDWPQVKARLEIWLRPENF
                     DKNGRQHKSLREL"
     gene            63774..64190
                     /locus_tag="VVL45_000076"
     CDS             63774..64190
                     /locus_tag="VVL45_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4186339.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQTEMLEQALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVFVQDLIVLPSYQRQGIGSSLMKEALGNFKEAYQVQ
                     LATEETEKNVGFYRSMGFEILSTYDCTGMIWINREK"
     gene            64984..66240
                     /gene="ltrA"
                     /locus_tag="VVL45_000077"
                     /pseudo
     CDS             64984..66240
                     /gene="ltrA"
                     /locus_tag="VVL45_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            66696..67874
                     /locus_tag="VVL45_000078"
     CDS             66696..67874
                     /locus_tag="VVL45_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4186340.1"
                     /translation="MSNSFVKLLVSQLFANLADIFFRVTIIANIYIISKSVIATSLVP
                     ILIGISSFVASLLVPLVTKRLALNRVLSLSQFGKTILLAILVGMFTVMQSVAPLVTYL
                     FVVAISILDGFAAPVSYAIVPRYATDLGKANSALSMTGEAVQLIGWGLGGLLFATIGL
                     LPTTCINLVLYIISSFLMLFLPNAEVEVLESETNLEILLKGWKLVARNPRLRLFVSAN
                     LLEIFSNTIWVSSIILVFVTELLNKTESYWGYSNTAYSIGIIISGLIAFRLSEKFLAA
                     KWESILFPLVAMEIVTLTILYFPNAQMFLLFSALVGMLSQLKEVPESVFLQETVEENH
                     LVNVYSVLEVISTLAFSVFVLLMSYITESFGISISFWLSAICLMIEAILIYIRRDYFK
                     "
     gene            68188..68319
                     /locus_tag="VVL45_000079"
     CDS             68188..68319
                     /locus_tag="VVL45_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEU4186341.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            68340..69890
                     /gene="dltA"
                     /locus_tag="VVL45_000080"
     CDS             68340..69890
                     /gene="dltA"
                     /locus_tag="VVL45_000080"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEU4186342.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFEDLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            69887..71131
                     /gene="dltB"
                     /locus_tag="VVL45_000081"
     CDS             69887..71131
                     /gene="dltB"
                     /locus_tag="VVL45_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEU4186343.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIVIDLRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMWGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            71145..71384
                     /gene="dltC"
                     /locus_tag="VVL45_000082"
     CDS             71145..71384
                     /gene="dltC"
                     /locus_tag="VVL45_000082"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEU4186344.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            71377..72645
                     /gene="dltD"
                     /locus_tag="VVL45_000083"
     CDS             71377..72645
                     /gene="dltD"
                     /locus_tag="VVL45_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEU4186345.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKEELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMNYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            72800..73240
                     /gene="adcR"
                     /locus_tag="VVL45_000084"
     CDS             72800..73240
                     /gene="adcR"
                     /locus_tag="VVL45_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HEU4186346.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            73240..73944
                     /locus_tag="VVL45_000085"
     CDS             73240..73944
                     /locus_tag="VVL45_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU4186347.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYSVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGNKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVSHA"
     gene            73937..74743
                     /locus_tag="VVL45_000086"
     CDS             73937..74743
                     /locus_tag="VVL45_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU4186348.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGIAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            74753..76258
                     /gene="adcA"
                     /locus_tag="VVL45_000087"
     CDS             74753..76258
                     /gene="adcA"
                     /locus_tag="VVL45_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HEU4186349.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGKEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNVAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
BASE COUNT        23194 a        13839 c        16562 g        22744 t
ORIGIN      
        1 ttaactattg ctggtttggg tggtatattg ggaaatatgc tagcgccaat cgtaataaaa
       61 tatttaaaat cgaatcaaat tgttggtgta tttctttttt tgaacggctc aagttggtta
      121 gtagcaattg ttataaaaga ctatacttta tcacttattt tatttttcgt ttgttttatg
      181 tctaaaggag tcttcaatat tatttttaat tcgttgtacc aacaaatacc tccacatcaa
      241 cttcttggta gggtaaatac taccattgat tctattattt cttttggaat gccaattggt
      301 agtttagttg caggaacgct tattgatttg aatattgaat tagtgttaat tgctattagc
      361 ataccttatt ttttgttttc ttatattttt tatacggata atggattgaa agaatttagt
      421 atatattaga aatgtttatg ttcattcaaa agcataatga ctataactga aaaagaaaag
      481 tgatatcttt aaggttgttc ttcttggtgg tgagattcgt gagacaaccc aagcttttgt
      541 cggaaagatt accaatgctt tgatggatag gatgtacttt agcaagatgt ttttagtggt
      601 aacggtatcg tggatggacg tgtaataacc tcttctttcg aggagtattt tactaaaaaa
      661 ctagccttgg agcgttcccc agaaacggac ttactcattg actcttcaaa gatttgggga
      721 gaagattttg cttcatctgt tccttgaaaa aagtcacagc agtcatcaca gacgatagta
      781 ctgaacaaaa ctatgaagag ttagaaattt atacgcaggt gattgtataa aggatctgga
      841 aatagataag aagttgatta gtattgacct aggtggtaca aatattaaga ttactgttct
      901 ttcaaatgac ggtgagattg aaactttgtg gagtattaca acagatacaa gtgagaaagg
      961 ttctcaaatt atatcggaca tcatcagttc tattaaaaat aaattgaccg aacggaatat
     1021 tcctgatagc gaccttcttg gaatcggtat gggaagttgc tcatcatact ttccttgtaa
     1081 atcatagggg ctataaactc tccgtctact tgtcctgcaa caattgaagt ctgctcaaaa
     1141 cgccgtccgc taatcttttc atagactttc tcccttttag gagcctagct ttctagtttg
     1201 ttctttgatt tttattgagt ataccactat tttactccct ctggcaaggg actttgtcta
     1261 tgtggaggga ttgggctcct atgtggtgga gcttttctgt tctttctgaa atatggtata
     1321 atagcactaa tcaatttcta ggaaaataga tacagaaagg ggctgaaaga tgtctcatat
     1381 tattgaattg ccagagatgc tggcaaacca aatcgcggct ggagaggtca ttgaacgtcc
     1441 tgccagtgtg gtcaaagagt tggtagaaaa tgccattgac gcgggctcta gtcagattat
     1501 cattgagatt gaggaagctg gtctcaagaa ggttcaaatc acggataacg gtcatggaat
     1561 tgcccacgat gaggtggagt tggccctgcg tcgccatgcg accagtaaga taaaaaatca
     1621 agcagatctc tttcggattc ggacgcttgg ttttcgtggt gaagccttgc cttctattgc
     1681 gtctgttagt gtcttgactc tgttaacggc ggtggatggt gctagtcatg gaaccaagtt
     1741 agtcgcgcgt gggggtgaag ttgaggaagt catcccagcg actagtcctg tgggaaccaa
     1801 ggtttgtgtg gaggatctct ttttcaacac gcctgcccgt ctcaagtata tgaagagcca
     1861 gcaagcggag ttgtctcata tcattgatat tgtcaaccgt ctgggcttgg cccatcctga
     1921 gatttctttt agcttgatta gtgatggcaa ggaaatgacg cggacagcag ggactggtca
     1981 attgcgccaa gcaatcgcag ggatttacgg tttggtcagt gccaagaaga tgattgaaat
     2041 tgagaactct gacctagatt tcgaaatttc aggttttgtg tccttgcctg agttgactcg
     2101 ggctaaccgc aattatatca gcctcttcat caatggccgt tatattaaga acttcctgct
     2161 caatcgtgct attttggatg gttttggaag caagcttatg gttggacgtt ttccactggc
     2221 tgtcattcac atccatatcg acccttatct agcggatgtc aatgtgcatc caactaagca
     2281 agaggtgcgg atttccaagg aaaaagaact gatgactctg gtttcagaag ctattgcaaa
     2341 tagtctcaag gaacaaacct tgattccaga tgccttggaa aatcttgcca aatcgaccgt
     2401 gcgcaatcgt gagaaggtgg agcaaactat tctcccactc aaagaaaata cgctctacta
     2461 tgagaaaact gagccgtcaa gacctagtca aactgaagta gctgattatc aggtagaatt
     2521 gactgatgaa gggcaggatt tgaccctgtt tgccaaggaa accttggacc gattgaccaa
     2581 gccagcaaaa ctgcattttg cagagagaaa gcctgctaac tacgaccagc tagaccatcc
     2641 agagttagat cttgctagca tcgataaggc ttatgacaaa ctggagcgag aagaagcatc
     2701 cagcttccca gagttggagt ttttcggaca aatgcacggg acttatctct ttgcccaagg
     2761 gcgagatgga ctttacatca tagatcagca cgctgctcag gaacgggtca agtacgagga
     2821 gtaccgtgaa agcattggca atgttgacca aagccagcag caactcctag tgccctatat
     2881 ctttgaattt cctgcggatg atgccctgcg tctcaaggaa agaatgcctc tcttagagga
     2941 agtgggcgtc tttctagcag agtacggaga aaatcaattt attctacgtg aacatcctat
     3001 ttggatggca gaagaagaga ttgaatcagg catctatgag atgtgcgaca tgctcctttt
     3061 gaccaaggaa gtttctatca agaaataccg agcagagctg gctatcatga tgtcttgcaa
     3121 gcgatctatc aaggccaatc atcgtattga tgatcattca gctagacaac tcctctatca
     3181 gctttctcaa tgtgacaatc cctataactg tcctcacgga cgtcctgttt tggtgcattt
     3241 taccaagtcg gatatggaaa agatgttccg acgtattcag gaaaatcaca ccagtctccg
     3301 tgagttgggg aaatattaaa agtataaaaa agtctgggaa aaattttcaa aatcaaaaaa
     3361 acgcataaaa tcaggtgttc aaaaaccttg attttatgcg ttttatcatg gaaatagtta
     3421 cttcattttt tcctaattct tttcgaaact ctttttaaac gacgtcagtt ttatcagtaa
     3481 tctcaaaaca gtgttttgag ctaattttgc cagttttgtc tgtaacatcg aagttgtgtt
     3541 ttaccactct gcgactggtt tcctagtttg ctctatgatt ttcacagagc attaaattgc
     3601 gattttgcca agtttcttta ttcgtctaaa agtagagtct gttctatgcg tctaatgtac
     3661 gaatcaggtt gaccatttca atagctcctt gtgcacactc agaaccctta tttcctgctt
     3721 tagtaccagc tcgttctatg gcttgttcaa ttgtatctgt cgttagcaca ccaaacataa
     3781 caggaatttc gctatttaaa ctgatttggg cgattccctt agatacctcg ctacatacat
     3841 aatcataatg acttgtattc cctctaatga cagctcccaa gcagataatt gcatcatatt
     3901 ttttactttt tgccattttt gatgcaatca gtggtatttc aaaagctcct ggaacccagg
     3961 ctacctctat atctttctcg tttacattct ctcttttgag attatctagt gctccagata
     4021 ataattttga agttataaat tcattaaatc tcgctacaac aatacctatt ttaatattgt
     4081 ttgctactaa attaccttca taagtgttca tttatttttc ctccatattt aaaatgtgac
     4141 ccattcgatt tttctttgtt tctaaataaa aactatcgta aggattggct tctatttcga
     4201 ttgatattct actggaaatg gtaattccat atttttctaa ctgttcaacc ttgtcaggat
     4261 tatttgtcag taaatgaagt gactgaagtc ccagatcttt aagcattttt gctccaatat
     4321 gatattctct taaatcacct tcaaagccta atgcaagatt ggcatcaagc gtatccatgc
     4381 cttgatcttg taaatgatag gcttttaatt tattgataag tccaattcct cgtccctcct
     4441 gtcgcaagta aagtaagaca cccgaaccat tctcaacaat cattttcata gccttatcga
     4501 attgctgtcc acaatcgcaa cgtaaagagc ctaaaacatc tcctgttaaa cattcggagt
     4561 ggacccgaca taatacattg gcttcatcct ctatatttcc cataataaga gcaagatgat
     4621 gttccccatt tagtttatct atatagctaa ttgctttgaa attaccgtat ctagtaggca
     4681 tattgacagt tgaaactcgt tctaccagct gatcatatac ttttctatat tcttgtaatt
     4741 ctttgatggt aattagtgga atgttgtgtt ttttcgagaa ctgaattaaa tcatctgttc
     4801 tcatcatttt gccatcatga ttcattattt cacaacatag gccacactct tttagtccag
     4861 ctaattttaa taaatcaaca gttgcttctg tgtgtccatt tctttctagg acaccacctt
     4921 tttttgcaat taaaggaaac atgtgtcctg gcctgcgaaa atcagagggt gttatatctt
     4981 cagctacaca catacgtgcg gtcagtcctc tttcctcggc agaaatacct gtggtcgttt
     5041 ctttataatc aattgaaact gtaaaagcag tcttatgatt atctgtattg ttttcaacca
     5101 taggtgaaag cattaattga ttagctaaac tttcgctcat aggcatacaa attaatcctt
     5161 tggcataagt agccataaaa ttaacatttt ctgttgtagc tgcttgtgca gaacaaatta
     5221 agtctccttc attttctcta tccttgtcgt ctataacaag aacaagtcgt cccttctgca
     5281 atgcttctaa tgcttcttgt atttttcgat attccattga ctgattatcc tttctgctaa
     5341 aatccatttt gatataatag ttccttagat atttctgatt ttggagagtt atccatcagt
     5401 ttttgcacat atttacctaa gatatcattt tcaagattta ctgtactccc gacttgttta
     5461 ctcttaagaa tggtttgttc caaggtatga gggataacag atactgaaaa gtttactttg
     5521 gagactttag cgacagtcag actaatgccg tcaattgtaa tagatccttt ttcaactatt
     5581 aaatctaaaa tttctttttg tgtgttgatt tgataccata cagcattatc atcttttttt
     5641 attgacgaga tttttcctgt accatcaatg tgtcctgtaa cgacgtgacc cccaagtcga
     5701 ccgttgacag ataaggctct ttctagattc acctcacttc catgttttaa tagagtaaga
     5761 gctgttcgac tccatgtttc attcattaca tcaactgtaa aggattgatg attgaaatga
     5821 gtaactgtaa gacagatacc atttactgct atactatcgc ctaaatggat atccgttaat
     5881 atttttgagg ctttaattga tagtttacaa ttacgagagt ctttctgtat tctttcaact
     5941 tttccgattt cttcaattat tcctgtgaac atggataaat cacttcactt tctatgagat
     6001 agtcatttcc tatttgagaa aatgcataag gtttcaatct aatagcgtca tttggcaaag
     6061 aaataccttc acctccgaca ggaaacttgg cactacctcc aaaaactttt ggtgcaatat
     6121 atattttcag ctcatcaaca atttgttgtt ccaaagcact ccaattcatt agactgcccc
     6181 cttctagaac taggctatca atctgcatgt ttcctagatg ttgcattaaa ctcgataagt
     6241 ctatatgatt gccttttttc tttatggaaa gtatttcaca gccatgattt tgatatagct
     6301 tcattttatt tttgtcttca gaggaagtgg caatgtaagt tttaatatca tttgctgttt
     6361 ttacgatttt agaggtaaga ggagttcgta aatgtgtatc gcatatgata cggataggat
     6421 ttttcccttc ctccaatcta catgtcagca aaggatcgtc ttgaataaca gtattgactc
     6481 ccaccataat tgcactaaca tggtgtcgta actgatgcac atgctttctt gcttcttctt
     6541 cagtaatcca tttggattga tttgttttag tggctatttt tccatccatt gacattgcat
     6601 atttcataaa aacatagggt acatgctggg taatatactt tctaaaactt tttattaagt
     6661 taagacactc attttctaaa attccaacag taacttgaag attattttcc tcaagtatct
     6721 ttactccttt tccagataca ataggattac agtctaggct tccaatgact actcttgtaa
     6781 taccactatc gattatagca tctatacagg gaggtgtttt cccgaagtga caacagggtt
     6841 caagtgttac ataaagcgtc gctccgacag gggattctct acagttttta agagcatttc
     6901 tctcagcatg tgggccacca aaaaactcat gataaccttg tccgataatg tgattatctt
     6961 ttacaataac tgcgccgacc ataggattgg gattgacgta accagcccct ttttgtgcca
     7021 gttttattgc taatttcata tattttgaat cgctcatctc gctacctcca aaaaaatata
     7081 ccttgaatag gggactactc aaggcataca aaagaaaact tatgcgatta acaaaaatgc
     7141 tctgaaatga caagtaatca tttcagagca cgcaaaaagc acaaatatac ttttatcttc
     7201 tttcatccag actatactgt cggctttgga atttcaccaa atcatgcctt tcggctcgtg
     7261 ggctatacca ccggtaggga atttcaccct gccctgaaga tagttattca attacagatg
     7321 attatagtac ttaattttga atatgtcaac agataaatac cgattgtttt tgatatactg
     7381 tatttgtgat aatcgattct cgctcctcgg ataaagaaaa tatgatatac tagataaacg
     7441 aaataagaga gaaggaatac tatgtacgca tatttaaaag gaatcattac caaaattact
     7501 gccaaataca ttgttcttga aaccaatggt attggttata tcctgcatgt ggccaatcct
     7561 tatgcctatt caggtcaggt taatcaggag gctcagattt atgtgcatca ggttgtgcgt
     7621 gaggacgccc atttgcttta tggatttcgc tcagaggatg agaaaaagct ctttcttagt
     7681 ctgatttcgg tctctgggat tggtcctgta tcagctcttg ctattatcgc tgctgatgac
     7741 aatgctggct tggttcaagc cattgaaacc aagaacatca cctacttgac caagttccct
     7801 aaaattggca agaaaacagc ccagcagatg gtgctggact tggaaggcaa ggtagtagtt
     7861 gcaggagatg accttcctgc caaggtcgca gtgcaagcaa gtgctgaaaa ccaagaattg
     7921 gaagaagcta tggaagccat gttggctctg ggctacaagg caacagagct caagaaaatc
     7981 aagaaattct ttgaaggaac gacagataca gctgagaact atatcaagtc ggcccttaaa
     8041 atgttggtca aataggagca gagaatgaca aaacgttgtt cgtgggtcaa gatgaccaac
     8101 ccgctctaca tcgcctatca tgatgaggag tggggccagc ccctccatga tgaccaagta
     8161 ttgtttgagt tgttgtgtat ggaaacctat caggcaggcc tgtcttggga aacggtactc
     8221 aacaaacgcc aagctttccg agaagtcttt catagctatc aaattcactc agtcgcagag
     8281 atgactgaca ctgaattgga agccatgctg gagaatccag ctatcattcg aaatagagcc
     8341 aagctttttg ctacacgcgc taacgcccaa gcctttctac agttacaggc agagtacggc
     8401 tcttttgatg cctatctttg gtcttttgtt gaggggaaaa ctgtcgttaa cgatgttcct
     8461 gattatcgcc aagcgccagc taaaacaccc ttatctgaga aattagccaa agatctcaaa
     8521 aaacgaggct tcaagttcac aggcccagtc gccgtattgt cttttctaca ggctgcaggg
     8581 ctagttgatg accacgagaa tgattgtgag tggaaaggtc ttaaatgatg tctaacaaaa
     8641 ataaggaaat tctgattttt gcgattctct atacagtcct ctttatgttt gatggcgtta
     8701 aattgctggc ttctttaatg ccatctgcca ttgcaaatta tcttgtttat gtagttttag
     8761 ctctatatgg ctccttcttg ttcaaggata gattgatcca acaatggaag gagattagaa
     8821 agactaaaag aaaattcttc tttggagtct taacaggatg gctctttctc attctgatga
     8881 ctgttgtctt tgaatttgta tcagagatgt tgaagcagtt tgtgggacta gatggacaag
     8941 gtctaaatca gtctaatatt caaagtacct ttcaagaaca accactactg atagctgttt
     9001 ttgcttgtgt cattggacct ctggtagaag aattattttt ccgtcaggtc ttattgcatt
     9061 acttgcagga acggttgtca ggtttactaa gcattattct ggtaggactt gtttttgctc
     9121 tgactcatat gcacagtttg gctctatcag agtggattgg tgcagttggt tacttaggtg
     9181 gaggccttgc cttttctatt atttatgtga aagaaaaaga gaatatctac tatcccctac
     9241 ttgttcacat gttaagcaac agcctctcct taatcatttt agctatcagt atagtaaaat
     9301 gaaatgagaa caggacaaat cgatttctaa caatgtttta gaagtagagg tgtactattc
     9361 tagtttcaat atactgtaat atgtgatgaa aatgccagta atgataccga gaaaaaagct
     9421 gagaaacttt tcccagcttt atttgttata gtcaaagaga atgacttgtt cctgtgcatc
     9481 tacatgagca tggaccccaa agggtacaat tgctcttgga gttgcgtggc cgacattcag
     9541 attatagaca atcgggatat tgctgtcaat gatatccaat agtgcctctt tatagtcgtc
     9601 atggaaagtt tcatccatag gttttccgac caagagtcca ttgatgaccg cgaatatgcc
     9661 agtgtccttt aaagttagca acatcttttt gaagtcttct ggcttaggct tttcttcgct
     9721 tgtttcgagc aagaggattt tcccttccca gtctgacaag tcagggaaaa gtttgtattt
     9781 ttggcagagt tccgtgctat ctgcgtatcg agagttgtca aagatatcgt agagggattc
     9841 gaggcaacca ccgaggattt tcccctcgaa ctgggcactt ccttgcaaca agtcaaaacc
     9901 tgtatttgta tgactgacac gaggtgttcc cagggccgtg ggactaaaat cagttcgttc
     9961 ctcataccaa acgtcactag ggcggatttc tgaaattctt cccgtctcaa tcaattcttt
    10021 aaagtagtga aggctatagg ctagcatttc tttgtctaat tcacaaatgt ctgctaaaaa
    10081 ggattgacca taaaaagtct tgattcctaa tttatgcaac atgaggtggt tcatggttgt
    10141 atccgagaag ccaagaaaaa ttttttgttt gataaccttt tggagttggt cattttcaaa
    10201 aagataaggt agcaagcgat aggtatcgtc tccaccgatg gcacatagga tcatgtcgat
    10261 gctatcatca gaaaaggcat gaatcaaatc ctctgcacga gcttcaggat ggtccttgat
    10321 aaagtctaat ccttttaacg aatggggcaa aaagatggga ttgagtccca gatccttgag
    10381 acgttggata cccaagtcca cttcgtgttt gacaaaatcc tctccgataa tgccactaga
    10441 taaactaaca ataccaatag tagaaaccat atctcatcct cctagaaata gattgagcct
    10501 attttatcac aaaatataag agaaagctat gtgggatttc agaagaatac ttttaatact
    10561 cttcgaaaat ctcttcaaac cacgtcagtt ttatttgcaa cctcaaagct gtgctttgag
    10621 caacctgcag ctagcttcct agtttgctct ttgattttca ttgagtataa ctcaaaaaag
    10681 taagtgaaaa agcaaccgca cctgcagttg cttttatcgt tttcttattc ttgatagcgt
    10741 gttggtcctg cacttgcact acggatattc aagcgtttct tgagatagaa gcgaagggct
    10801 aggagggcta ctccaataat agctagtggc aatggatcca gtactgggtt aaggctagct
    10861 ggtaggaagc ttgttgcaaa gaagacaagc aaccaaagga acatagaagt taggataact
    10921 agtacagatt tccagaaagg tggacgttga ctgcgatcca tatctggtcc atagtattgg
    10981 taaacaaagt agtacatcaa gtagaaggca aatccaccaa ccagtccaac taatagaaga
    11041 gtaatcaatc catagccaaa agcttgatct gccgcaaaga aggttgtgag ggcgctgaca
    11101 agggcaaaga ggctagtgat gaaaagagct gagtccataa tcatcagttt tggatcatca
    11161 ttttcttttg gatgctcttt ttcgtactgc tctttgacag tgaagctatg agcccaatga
    11221 gttggtgcgc cgtataggga acgggcagtt gtacctttag attgctcctc aaggatttgg
    11281 ggaatgactt cctcaaaaat agtctggatt tcagcgtctg ttttcccatc tttgatgaat
    11341 tgttgggtag caatgtggac aaactcttgg tttttcttag ttaatttttg tagatcaatt
    11401 tgagacatag gaactcctct tagaaccatt ttttatggat gagatagaga gtgagcgaga
    11461 cactcatagc aaaggcgata aagacgatta accagaaggc atttggctct ccgtttaggg
    11521 gaatttcatt atccttaaag ttcatcccgt aggcagaaaa gaccatggtt gggatggaca
    11581 tgacgatggt cacaagggcc aaggttttca tgatgttgtt ctggttgtta gaaatgatag
    11641 aggcaaaggt ctctgtcata gaatgcaaga cgtttccata aatatctgcc atctcgatgg
    11701 cctgttgggt ttcaatcagg gtgtcttcaa gcagatcttc gtcctcaagg tatttcttga
    11761 tattgctggt tgaactggtc aatttcttaa tcacgcgctc atttgttttg agggaggcct
    11821 tgaaatagac gatagttttt tccaattcca tgagctcaat caattcttca ttacgagttg
    11881 attgatgcag ttgactttcg atttgttcac tcttgcggtc gattgaacga agggctgtta
    11941 ggtaaagctc tgcattgcga taaagaattt gaaagataaa acgtgaacgc atgaaggtat
    12001 agaaattacg caatcgacgg ttgataaaga catcaaggac aggtagtggt tccaaacacg
    12061 tagtgataat ggtttcctca gtgatgataa taccaagcgg gatggttacg tagtaggtgc
    12121 ggttatttct ttcctccgtg accggcacgt ctacgataat cagggtatac tcgtcttcaa
    12181 tggtaatacg agacatttct tccgcatcga gcggtgctcg aaggtcggca atatcaatat
    12241 cgaaggtgtt ggcgatttcg agtgattcat tttgagtcgg attgacgaga ttgatccaag
    12301 tacccggttc aagcgtatcg atctctttaa attcagttgt tgtagagaga aaaacttgtt
    12361 tcatatccct tatcctttct catttttcag attttttcac actgtactat tatactacaa
    12421 aatcggcctt ttgggtaata gaatctcact ttttttggta taatggtaag caataatgga
    12481 ctagaaagaa caaagatgca agataaaatt gtcattcatg gggcgcgtgc ccataattta
    12541 aaaaatattg atgtggagat tccgcgagac aagttggttg tcgtgactgg cttgtcaggt
    12601 tcagggaagt ccagtctggc ttttgatact ctctatgcgg agggacaacg tcgctatgta
    12661 gagagtttgt ctgcctacgc tcgtcagttc ttgggaaata tggaaaaacc agatgtagat
    12721 gccattgatg gtctcagccc agctatttcc atcgaccaga aaacgactag taaaaatcct
    12781 cgctcgacgg tgggaacgac gactgaaatc aatgactatc tgcgtctcct ctacgcacgt
    12841 gtggggacgc cttactgtat caacggacat ggagctatca atgcttcttc tgtggagcaa
    12901 atcgttgata aggttttgga gttacctgaa cgccagcgct tgcagatctt ggctcctgtc
    12961 atccgcaaga aaaaaggcca acataagagt gttatcgaga aggttcagaa agacgggtat
    13021 gttcgtgttc gtgtggatgg ggaagtctat gatgtgaccg aagtgccaga gttgtctaag
    13081 agcaagcaac acaatatcga tgtcgtggtt gaccgtattg ttatcaagga gggcattcgt
    13141 agccgtctct ttgattccat tgaagctgcc cttcgtatcg cagaaggtta tgtgattatc
    13201 gacaccatgg acgactctga gttgttgttc tctgagcatt atgcctgtcc agtttgtgga
    13261 tttactgtcc cagagttaga acctcgtctc ttctctttca atgctccctt tggctcttgt
    13321 agtgagtgtg acggcttggg gattaagctg gaggtggata ctgatttggt agtgccagat
    13381 gccagcaaaa ccttacatga gggagcgctg gcaccttgga atcctatctc atccaactac
    13441 tatccaaaca tgttagagca ggccatgaaa gtctttggag tggctatgga taagcctttt
    13501 gaggacctgt cagaagaaga taagaacttg attctctacg gctcagatgg taaggaattc
    13561 catttccatt atgaaaatga atttggtggt gtgcgcgata tcgacattcc gtttgaggga
    13621 gttatcaata atatcaaacg tcgttaccat gaaaccaata gcgattacac tcgcactcag
    13681 atgcgtctct acatgaatga gctgacctgc ggaacctgtc aaggctatcg tctcaatgac
    13741 caggccttgt ctgtccgtgt gggcggccag caagggccac atattggaga aatctcagac
    13801 ctgtctatcg ctgaccactt ggacttggtg agccagttaa ctttgtctga aaatgaagcc
    13861 atcattgctc gtcccattct caaggaaatc aaggatcgtt tgaccttcct taataatgtg
    13921 ggtcttaact atctaaccct gtcacgttca gcaggaaccc tttcaggtgg ggaaagtcag
    13981 cgcattcgtt tggcaaccca gattggttcc aacctatcag gtgtcctcta tatcctagac
    14041 gagccgtcaa tcggtcttca ccagagggac aatgaccgcc tgattgccag tctgaaaaag
    14101 atgcgtgact tgggcaatac tcttatcgtg gtggaacacg acgaagatac catgcgtgaa
    14161 gctgattatc tgattgacgt tggtcctggt gccggtgttt ttggtggtga gattgttgca
    14221 gcaggtacac ctaagcaagt ggctcgcaac agcaagtcta tcacaggtca gtacttgtca
    14281 ggcaaacgtg tcatcccagt accagaagag cgccgtgtcg gaaatggtcg ttttattgaa
    14341 gtgacaggag cgcgtgagaa caacttgcaa aatgtcacgg ctcgctttcc actaggaaaa
    14401 tttatcgcag tgacaggtgt gtcgggttca gggaaatcga ctctaatcaa cagcattctc
    14461 aaaaaagcca ttgcccagaa gctcaaccgt aattcagaca aacctggtaa gtttaagact
    14521 attacaggga ttgagcatgt cgaccgtttg attgatattg accagagccc tatcggacgg
    14581 acgccgaggt ctaacccagc tacctataca ggagtttttg acgatatacg tgaccttttt
    14641 gctcagacaa atgaagccaa gattcgaggc tacaagaagg ggcgcttcag tttcaacgtc
    14701 aagggaggtc gctgtgaagc ttgctcaggt gacgggatta tcaagattga gatgcacttc
    14761 ttgccagatg tttatgtggc ttgtgaagtt tgccacggaa cccgctataa cagtgaaacc
    14821 ctagaagttc actacaagga aaagaatatt tcgcaggtct tggatatgac ggtcaacgat
    14881 gcggtagaat ttttccaaca cattccgaaa attcaacgca aactccagac catcaaggat
    14941 gtaggtctag gctatgtgac cttgggacag ccagctacca ccctttctgg gggagaagcc
    15001 caacgtatga agttagctag cgaactccac aaacgctcga caggaaaatc tttctacatt
    15061 ctggatgagc cgacgacagg gcttcataca gaggacattg ctcgcctgct caaggtttta
    15121 gctcgctttg tagacgccgg caatacagtc ctcgtcatcg agcacaatct ggatgttatc
    15181 aagacggcag accatatcat tgacttggga cctgagggcg gtgtcggtgg tggaaccatc
    15241 atcgtaacag gaactccaga agaagtagct gccaacgaag ccagctatac aggacactat
    15301 ttgaaaggaa agttacatca tgaataaacg cgtacaagca tttctagcta aaatgcaaga
    15361 aaaagaacta gatggtatca tcatcaacaa tcttaaaaac gtctattatt tgactggttt
    15421 ttggggctca aacggaacag tctttatcag ccgtgaccgt caggtcttag tgacagactc
    15481 tcgctatatc atcgcagcta agcaagaaac cagtggtttt gagattgtgg ctgatcgtga
    15541 tgaattggct gtcattgcag gaattgttaa ggacatgaac ttgactcgta tcggttttga
    15601 agatgagatt tcagtgtctt attaccaccg tatgcaggca gcttttgcag gtttgaactt
    15661 gcttccacaa actcagtttg tggaaggtct tcgtatgatt aaggatgagg cagagattgc
    15721 agcgattcgc aaggcttgtt ctatctcaga ccaagctttc cgcgatgcgc ttgactttat
    15781 caaaccagga aaaactgaaa ttgagattgc caacttcctt gatttccgca tgcgtgagtt
    15841 gggagcatct ggcttatctt ttgatacgat cctagctagc ggtatcaatt cttctaaacc
    15901 ccatgcccat ccaatgcaca aaccagtgga gttgggagaa gccatcacca tggacttcgg
    15961 ctgtctctat gaccactatg tcagtgatat gacccggact atctatctag ggcatgttag
    16021 cgatgagcag gcagagattt acaatacggt tctaaaagct aaccaagcct tgattgacca
    16081 agctaaggca ggcttaggtt tccgtgactt tgacaaaatc cctcgtgata ttatcattga
    16141 ggcaggttat ggtgactact ttactcacgg cattggccac ggtattggtc tggatatcca
    16201 tgaggaaccc tactttagtc agacttctac agaaactatt aagacaggta tggccttgac
    16261 cgatgaacca ggtatctata tcgaaggcaa atatggcgtt cgtatcgagg atgatatcct
    16321 gattacagag acaggttgtg aattattgac cctagctcca aaagagttga tagtcattta
    16381 gttgtttgtc aagaaaaaag tacgaaaatg acgaaaaaag tcaaaaaaaa ttaaaaatag
    16441 gtcgcaagtc ggatgttttt tatggtataa tagactaaac tgatagtaac atgtagcgaa
    16501 aggggtaggt acatgattaa aatttataca gtctcaagtt gtactagctg taaaaaagca
    16561 aaaacctggc tcaatgccca ccagttaagt tataaagaac aaaaccttgg taaagaagga
    16621 attacgagag aagaattact ggatattcta accaaaacag ataacggaat agccagcatc
    16681 gtttcgtcta aaaatcgcta tgccaaagcc cttggagtgg atattgaaga tttgagtgtc
    16741 aatgaagttc tcaatctgat tatggaaaca ccgagaattt taaagagccc aatccttgta
    16801 gatgaaaaac gcctgcaagt tggctacaag gaagacgata ttcgtgcctt cctaccacgc
    16861 tctgtccgta atgtagaaaa tgcagaagca cgtttgcgtg cagctctata aacatcaagg
    16921 ctgggagcac ttcccagtct tattctattt taatttcaaa aagaaagaag aaagaaatga
    16981 aaaaaatagt tcttgttagt ctagctttcc tttttgtcct ggttggttgc ggacagaaaa
    17041 aagaaactgg accagctaca aaaacagaaa aagatacgct tcagtcggca ttgccagtta
    17101 ttgaaaatgc tgagaagaat acagttgtaa ctaagacttt ggtcttgccc aagtcagatg
    17161 atggtagcca gcagacacaa acaattactt acaaagacaa gacttttttg agtctagcta
    17221 tccaacaaaa acgtccagtc tctgatgagt tgaagactta tattgaccaa catggagtgg
    17281 aggaaactca aaaagctctt cttgaagcgg aggagaagga taagtctatc attgaagctc
    17341 gtaaattggc aggtttcaaa cttgaaacaa aactattgag cgcaacggaa cttcaaacaa
    17401 cgactagttt tgattttcaa gttctggatg tcaagaaggc ttcccagttg gaacatctga
    17461 agaatattgg tttggaaaat cttttgaaaa atgaaccaag caaatatatt tcagatagat
    17521 tggcaaatgg cgcgacagaa caatagaaaa tccaaataaa tagactgcct gaattgtaga
    17581 aggtaaggaa ttatgctata atggtactat tctaaggaaa gagggtgtta gaatgggatt
    17641 tactgaagaa acagtacgtt ttaaattgga cgattccaat aaaaaagaaa ttagcgaaac
    17701 tttgacagat gtttatgctt cgttgaacga taagggttac aacccaatta accaaatcgt
    17761 aggttacgta ttgagtggag accctgccta cgttcctcgt tataataatg cacgaaatca
    17821 aatccgtaag tatgagcgtg atgaaatcgt tgaggaattg gttcgctact acctcaaagg
    17881 acaaggagtc gatctataac ctatgagaat tatgggattg gacgtcggtt caaaaacggt
    17941 aggggtggcg attagcgatc cgcttggttt tacagctcaa gggcttgaaa tcatccagat
    18001 aaatgaagaa caaggccaat ttggttttga ccgcgttaag gaattggttg atacttacaa
    18061 ggtggaacga tttgtagtgg gcttgcctaa aaacatgaac aatacaagtg gaccgcgcgt
    18121 agaagctagt caagcctacg gagcaaagct agaagagttt tttggtttac cagtagacta
    18181 tcaggatgaa cgcttgacaa cagtggctgc tgagcgcatg ttgattgaac aagcagatat
    18241 cagtcgcaat aagcgcaaga aagtcattga taagttagca gctcagctga ttttacaaaa
    18301 ttatttagat agaaaatttt aatataaagg agaggctatg tcacacgatc ataaccacga
    18361 ccacgaagaa cgtgaactaa tcacactagt agatgagcaa ggaaatgaaa ccttgtttga
    18421 aatccttttg acaattgatg gaaaagaaga atttggtaaa aactatgttc ttctagtacc
    18481 agttaacgca gaagaagacg aagacggaca agttgaaatc caagcttact cattcatcga
    18541 aaacgaagat ggaacagaag gcgaattgca accaatccca gaagactcag aagacgaatg
    18601 gaacatgatt gaagaagtct tcaacagctt tatggaggag tgatacagtc tgggagactg
    18661 tatcagccca actcccagaa atggggaaga gttggaacgt ccagtgaaca tttttacccc
    18721 aactcccaga aatggggaag agttggaacg tccagtggat gtttttagcc catattcaca
    18781 ttcatagtag ctagttatta gctaaccagg taagaggtcg ggacgaaaat cctggccttg
    18841 tttttattag taaccatact ttaatgcgat aattgattgg acttttgtca aggagatgtg
    18901 tatgtttgaa gtagaagaat ggctccatag tcggattggt ttgaattttc gatcaggttt
    18961 gggtcgaatg cagcaagcgg tggatttgtt aggaaatcct gagcagtctt accctattat
    19021 ccacgtaaca gggactaatg ggaaaggatc taccattgct tttatgaggg aattatttat
    19081 ggggcatggc aaaaaagttg cgacctttac ctcccctcat atcgtctcta tcaatgaccg
    19141 aatctgcatt aatgggcagc ctatagcaga cgcagacttt atccgtttga ctgatcaggt
    19201 caaggagatg gagaaaacgc ttctgcaaac tcctgcccag ttgtcctttt ttgaattgct
    19261 gaccttggtt gcttttcttt attttaggga gcaggaggtg gatttggttt tattagaagt
    19321 gggaattggt ggcttacttg acacgaccaa tgtggtaact ggagagtttg ctgtcatcac
    19381 ctccattggg cttgaccatc aagaaacctt gggtgatagt ctagaagcaa ttgcagagca
    19441 gaaagctggt attttcaagg ctggtaagaa ggcagtgatt gcgaaattgc ctccagaagc
    19501 taggcttgcc tgtcagaaaa aagccgaatc tttagctgtt aacctttatc aggcaggtca
    19561 agatttttta atgctgaatg gtgatttttc aagctcttta ctaaatcttt cacagctgaa
    19621 cataggctta gaaggagtct atcagcagga gaatgcagcc ttggcgttgc aaacttttct
    19681 tctttttatg agagaaagaa aggaagctgt tgatgaacag gctgtaagaa aggccttgga
    19741 acagacccat tgggctggtc gcttggagcg tattcgccca cagatttatt tggatggtgc
    19801 tcataacctc cctgccttga ctcgcttggc tgagtttatc aaagaaaaag agcaggaagg
    19861 ctatcgacct caaatcctct ttggatcctt gaaacgtaag gattatcaag ggatgttggg
    19921 ttatctgact gaaaaattgc ctcaggtgga actcaaggtg accggctttg actatcaggg
    19981 ggctttggac gaaagggatg tgacaggtta cgatatagtt tcttcttacc gagaatttat
    20041 cagcgatttt gaagaaaggg cagacgctca agacttgctg ttcgttacag ggtctctcta
    20101 ttttatctca gaagtacggg gctacctgct ggaccgtgag cagataaatt gactccattt
    20161 tttgaacttg ttatactagg ggaattgctg gttagagaaa atttctctaa attggtagca
    20221 gaaaggaaat tcatcatgaa attaaaaaga ttcacacttt ctcttgcttc tctagcaagt
    20281 tttagtctct tagtagcttg ttcacaaaga gctcaacagg ttcaacagcc tgttgctcag
    20341 cagcaggtcc aacaacctgc tcaacagaat accaatactg caaatgcagg aggtaaccaa
    20401 aatcaagcgg ctccagtaca aaaccaacct gttgctcaac cgaccgatat tgatgggact
    20461 tatactggtc aggatgacgg agaccgtatc actttagtgg taactggaac gactggtaca
    20521 tggactgagc tcgaatctga cggggatcag aaagtcaaac aggttacatt ggattcagca
    20581 aatcaacgca tgattattgg cgatgatgtc aaaatttaca ctgtaaacgg taatcaaatc
    20641 gtcgtagatg atatggatag agacccatcg gaccaaatcg ttttaactaa ataagactat
    20701 gcaagtagaa agggctggat ttttcagcct ttttactttt acctagaaaa taatcataaa
    20761 tacttgcctt ctaccgaata cctgcgttat actagtatca attacttgtt tttagcattc
    20821 aaactatcaa ggaggggata tgaaatatag gaaatttcaa ttattgatgt ccaagtatgg
    20881 ctttagtctt tcgattatgc tacttgaact ttgtcttgtt tttggtctct ttctttattt
    20941 aggacgcatg gctcccattt tatggattac tgtcctcatt ctactgagta tcatcacaat
    21001 catttcgata gtcaaccgta atacgactcc tgagaataag gtaacctggt tgttagtagc
    21061 ctttgtgcca gtatttggtc ccttgctcta tctgatgttt ggtgaaaggc gattgtccaa
    21121 aaaagaaatc aaacaactga agaagctagg ctctatgcat ttccaagaag caaatagcca
    21181 gctactaaaa gagaaattaa aagaaagtga caaggcagct tatggagtca tcaagtcctt
    21241 attgagtatg gataccaatg ctgacatcta tgatcaaact gcctctacat tttttcctaa
    21301 cggagaagct atgtggaaaa agatggtaga agatcttaaa aaggctgaga aatttatttt
    21361 cttggaatat tacattatag aagaaggttt gatgtggaat cgcatactag atatactaga
    21421 gcaaaaggta gctcagggtg tagaggttaa gatgctctat gatgatatcg gctgtatggc
    21481 tactttaaca ggagattatg cacatcgact tcgtcagctg ggcatcgagg cccataaatt
    21541 caataaagtt attcctcgtt tgacagtggc ttataataac agagatcata gaaaaatatt
    21601 gattgttgat ggtcagatag cctatactgg tggggtcaat ctggcagatg agtacattaa
    21661 ccacgtcgag agatttggtt attggaagga tagtggaatt cgcttagacg gactagcagt
    21721 aaaagctctg acacgcttat ttttgaccac ttggtacatt aatcgaggag aaattagtga
    21781 ttttgatcaa tatcatttag aaaatcattc tatcccgagt gacggtttaa ccattccata
    21841 cggaagtgga cccaagccaa tttttcgagc gcaggtaggg aaaaaagttt atcagagttt
    21901 aatcaatcaa gcaacagaat cggtctatat tacgacacct tatttgatta tagattatga
    21961 tttaacagag acaatcaaaa atgcagctat gagaggggtc gatgttcgaa ttatcacccc
    22021 ttacatacca gataagaagt tcattcagtt agtcacgaga ggagcttatc ccgaccttct
    22081 ttctgctggt gttcggattt atgagtatag tccaggtttt attcatagta agcagatgtt
    22141 ggtagacgaa gattttgcgg tggtggggac aatcaatctc gactaccgga gcttggtaca
    22201 ccattatgaa aatgcagtct tactctataa aactccttct ataagggaaa tcgcccgaga
    22261 ttttcgaaat atatttgcag attctcagga agtctatcct cattctatca aaacgagctg
    22321 gtatcaaaag cttgtaaaag aaatcgccca gctattcgcc cctatcttat aagaaatcta
    22381 ggacttagga tacaaaccag acctattttt cttgtctttt acgaatagaa agaaagagga
    22441 ggggaattca tgctaactaa ggaagaagtg aatgatatga ttgagttcaa acagacacat
    22501 ttacaccgct ttcgggaaat tgaaaccttt gtgaaactat gcaaaaaatc actcaaacag
    22561 ccatcgcaag ctgacaatcc taggactttt tgatatacta agacagataa attgaacagg
    22621 agaaacgata tgagtgtgta tggtagagta gaagaagttc ataaggagaa tcgtgaacct
    22681 ctagaatacc aaatcgaaca agaatcgcat catcgtgaat caagtcgtct tcctttggtg
    22741 aaaattttac tatggagtac gcttgtaacg gggataactc taggagtacc gctattactc
    22801 gacttaatga gtgcacaaga agtgcaggat ttttatgcag gttgggcgct tcatcagaca
    22861 gggaagattt acagcgacta ttatggaagt caaggtttgc tttattattt gctgacttac
    22921 gtgagtcagg gcggattttt ctttgccatc tttgagtggt tagccttggt agcaggagga
    22981 tttttccttt ttagatcagc ggacaccttg acagagcaag gagaccaagc tggacatctg
    23041 gtgactattt tttacatgct agttacaggt cttgcttttg gtggaggcta tgcgactctt
    23101 ttagcgcttc ctttcttatt cgcagccttt agtttagttg cggcttacct aagcaatcca
    23161 agccatgata agggatttgt acggattggg ctagctttgg caggcggatt tttctttgct
    23221 cccttatcat cgctcctgtt tattgctgta gtgagtttag gcttgttggt ctttaacctt
    23281 gggcatagac gctttgcgca tgggttttat cagtttcttg cagtggcttt aggtttttca
    23341 cttgtctttt atccaactgc ctactatagt gctgcaacag gaagttttgg ggatgcgatt
    23401 agtggtattc gttatcctat tgacagtatt cgctttgatt ttacttctaa aattttagag
    23461 aatatgtttt tttatggctt gttgtccctt ggtttgggat ttgtggtttg tatcttttta
    23521 ggtctctttc aatccaaacc ctttaaacta tacgtcattt cggttcctgc aagtcttgtg
    23581 gtaattttag gtttgatttt acttttcttt tcacaagagc ctctgcacgc ttcttatttg
    23641 atggtcgtct tccctgtttt cctactttta ttggtaacca atattaagag tcaacagagg
    23701 gggcgtagtg ctagaagaag ccgaagagaa acgccagtta gcctatggag tcgtttcttc
    23761 aaaggaaatc tatatctgct agtttttggg tttgtctatc ttttgtctgt tccttttttg
    23821 atgaagtttg tcctttatcc agtaccttat caagaacgta atcgtcttgc tgatttggta
    23881 aaagaggaga caaatacgga agatgctatc tatgcatggg atgatactgc gactctttat
    23941 cgtaagagtg agcgcttgtc gccatcggcg attttgtccc cgttgcacta tacagcaact
    24001 gaggaaaatc gtaataagtt acttaatgac ttgaaagaaa aacaacctaa ggtgattgtg
    24061 gtaaatgata aggtggtagt ctggtctgaa gtggaaacac tcttaaaaga aaattaccaa
    24121 caagtaaaga ctgattactc agagtttaaa gtctataaaa ttaaataacc aaatcaatat
    24181 cttgtgtatt tttaaaaatt ttaggatttt taacacaaga tattgatttt tctttttaga
    24241 gtggtataat actttttaga aagaacattt tagaaaagag catgcatatg attgcactag
    24301 aagaaaaaat tacaattttg ccaactctct tcgtcgagaa acgagatggg agacgtgttg
    24361 tatttgatgt ggacaagatt gacaaggctc tccacaaggc ggctgacaag gttatggatg
    24421 tgacacccct ggttgaaaaa tgcctcaatg atctgactga gcgaattatt acagaaattc
    24481 atagtcgctt tccacaggga attaagattt acgaaattca aaatatcgta gaacatgaac
    24541 tccttgaagc caaagaatat gcgctggctg aggagtatat tacttatcgg acacagaggg
    24601 attttgagcg ctcaaaagcg acggatatca actttagtat tcataaactt ctcaacaaag
    24661 accagacagt tgtcaatgaa aacgctaata aagacagtga tgtctttaac actcagcgtg
    24721 atttgacagc agggattgtt gggaaatcaa tcggactgca aatgcttcct aagcacgtag
    24781 ccaatgccca ccaaaagggg gatatccact atcacgattt ggactacagt ccctataccc
    24841 ctatgaccaa ctgctgtttg attgatttta agggtatgtt ggaaaatggt tttaagattg
    24901 gaaatgcaga ggtagagagt cccaagtcta tccagactgc gacagcacag atttctcaaa
    24961 tcattgccaa cgttgcttct agccagtacg gtggctgttc agctgaccgt atcgatgaaa
    25021 ttttggcgcc ttatgcagag aagaattatc aaaaacatct caaagatgca gaagagtggg
    25081 tattgcctga aaaacaggaa gattacgctt ggaagaaagc gcaaaaggac atctacgatg
    25141 ccatgcaatc tcttgagtat gaaatcaata ctctcttcac ttcaaatgga caaacacctt
    25201 ttacttcgtt aggttttggt ctgggaacca gtcgttttga acgagaaatt caaaaagcta
    25261 ttttaaacat tcgcatcaag ggtcttggtt cagaacaccg tacggctatc tttcctaaac
    25321 ttatctttac gcttaaaaga ggcctcaact tagaggaagg aactcccaac tatgacatca
    25381 agcagttggc tctagagtgt gcaaccaagc ggatgtatcc agacgtcttg tcttatgata
    25441 agattgttga tttgacaggt tctttcaagg tgcctatggg ctgccgttct ttccttcaag
    25501 ggtggaagga tgaaaatggt gtagaagtca attcaggtcg catgaatctg ggtgttgtga
    25561 cggttaatct gcctcgtatt gctcttgagt ctgaaggtga tatgaataag ttctgggaaa
    25621 tcttcaacga gcgaatgaat atcgcagaag atgctcttgt ttaccgtgtc gaacgcacta
    25681 aagaggcgac accagcgaat gctcctattc tttatcagta cggtgctttt ggccatcgtc
    25741 taggtaaaga agaaagtgtt gaccagctct ttaagaatcg tcgtgcgacc gtttcgctgg
    25801 gctatatcgg cttgtatgaa gtagcgacag ttttctttgg taacagctgg gaaagtaatc
    25861 cagatgctaa ggaattcacg ctagacatca ttcacgatat gaaacgccgt gtagaagagt
    25921 ggtcagacca atatggctac catttctcta tctactcaac accatccgaa agtctgacag
    25981 accgtttctg ccgactagat atagacaagt ttggctctat tcctgatatc acagacaagg
    26041 aatactacac caactctttc cactacgatg ttcgtaaaaa tccaacaccg tttgaaaaat
    26101 tggactttga gaaagtctat ccggaagcag gtgcgtcagg tggtttcatc cattattgtg
    26161 agtatccagt ccttcagcaa aatccaaagg ccttggaagc tgtctgggat tatgcttatg
    26221 accgtgtagg ctatctaggc accaatactc cgattgaccg ttgctacaag tgtgactttg
    26281 aaggggattt tgaaccaact gagagagggt ttgcttgtcc aaactgtggc aatagcgacc
    26341 ctaaaacagt agatgtggtg aaacgaactt gtggctacct aggtaatcct caagcaagac
    26401 cgatggtcaa cgggcgtcac aaggaaatcg ctgcgcgtgt caaacatatg aatggttcaa
    26461 cgattaaaat agctgggcat caagtaacaa attagaaaga aatgaaatgg gaaaatatca
    26521 actagacgat aaggggcgcg cacaagtgac ccgttatcac gagaaacact ctaaaggtgg
    26581 agctggtaag aaagaacgct tgcttagctt cagagaacaa tttttaaaca agaacaagaa
    26641 aaaataaaag tgagagccag ctctcgcttt tctcatagtg ggaggtaagg atggaattac
    26701 gcagaccaag attagcggat aagaaagctg ttttagatat gatgacagag tttgaaaaat
    26761 ttcagtcgcc tcacgacggc ggtttctggg atacagagaa ctttgtgtat gaagactggt
    26821 tagaaagcaa tcaggaacag gaaatgggga ttaatctgcc tgaaggatgg gttcctgcaa
    26881 ttcagttagt ggctttttct gagaaaggtc aagcagttgg atttcttaat ctccggttgc
    26941 gcctcagtaa ctttctacta gaagaaggtg gccacattgg ctactccatt cgtccatctg
    27001 aaagaggcaa gggttatgca aaagagactc tccgtcaggg cttgcaagtt gctaaggaaa
    27061 agaacatcaa gaaagctctg gtgacctgta gtgtgaataa tcctgctagc agagcagtca
    27121 ttctagcaaa tggtggaata tttgaggatg ctcgcaatgg agtcgagcgt tattggatag
    27181 aggtagcgaa tgaataatcc aaaaccacaa gaatggaaaa gcgaggaact tagtcaaggt
    27241 cgtatcattg actacaaggc ctttaacttt gtggacggcg aaggcgtgcg caactctctc
    27301 tatgtatcag gctgtatgtt tcactgcgag ggatgttata atgttgcgac ttggtctttt
    27361 aatgctggca ttccctatac agcagaatta gaagagcaga ttatggcaga ccttgcccaa
    27421 ccctatgttc aaggcttgac tttgctggga ggggagcctt ttctcaatac tgggattctc
    27481 ttgccacttg ttaagcggat tcggaaggaa ttgccagaca aggacatctg gtcctggacc
    27541 ggctacactt gggaagaaat gatgttggaa actccagata aactggaatt cttgtcactg
    27601 attgacattc ttgtcgatgg aagatatgat cgaactaaga gaaatcttat gctccagttt
    27661 cgaggttcat ctaaccaacg aattatcgat gtgcaaaaat cgctcaaaag tgggcaagta
    27721 gtgatttggg acaagctcaa tgacggaaaa gaaagctatg aacaggtgaa gagagaatga
    27781 agaaaaagga cttagtagac caactagtct cagagatcga gacggggaaa gtcaggacac
    27841 tgggaatata cggtcatgga gcttcaggta aatcaacctt tgcacaggaa ttgtaccaag
    27901 ctttagattc tactacagta aatttgctag agacagatcc ttatatcacc tcaggacgcc
    27961 atctggtagt acccaaggac gcgccgaatc aaaaggtgac agccagtctg ccagtggcgc
    28021 atgaactgga gagtttgcag agagatatcc ttgccttgca ggcgggtatg gatgtcttga
    28081 caattgaaga accttggaag gctagtgagg tcttgtctgg agccaaacca attttgattg
    28141 tcgaagggat gtctgttggc tttctaccca aggaactctt tgaaaaaacc atctgtttct
    28201 acacggatga ggagaccgaa ttaaagcgac gccttgctag agatacgact gtgagaaatc
    28261 gcgatgcatc ctttatattg gctagccatc agatgagacg ggagcagtat ctgcgatact
    28321 ataaagaaac tgagtctaag gcggatatct tagtggacca atcagaagat aaatttgatg
    28381 tcaagaggac ttaacttatg tatagaagaa atatagaaga aaatccctaa ttttagaaag
    28441 aaaaagtagg aaaacagttt catcccaaaa aaacgaaaaa acagcaacaa atcccttgca
    28501 atcgcagggg ctttgtgtta ttctattatg gtgctgtaaa ttacagcctt agttttgatg
    28561 caagaggttg cgacacgctc ggttgcattg ccacgcaaca ccgcgtcggt tttcttgtgg
    28621 agctagccta ttatcttaaa tagacgaaaa ggagaaaaag atggcaaaca aaaaaatccg
    28681 tatccgtttg aaagcttacg aacaccgtac gcttgacaca gcggctgcaa aaatcgtaga
    28741 atcagctact cgtacaggtg cacaagttgc gggtccaatc ccacttccaa ctgaacgtag
    28801 cctctacaca atcattcgtg cgactcacaa atacaaagac tctcgcgaac aatttgaaat
    28861 gcgtacacac aaacgtttga tcgatatcgt taacccaact caaaaaacag ttgatgcctt
    28921 gatgaaattg gatcttccaa gtggtgtaaa cgtagaaatc aaactttaat ctaaaatata
    28981 aaagagcaga ggctggtgtt tcaatctaat tgaacacggg ctaaactcgg tgtgaaaaag
    29041 ataaacttcc tagtgtctgc tagacactgc gtcagtttcc tattttcacg ttgagtttga
    29101 cgccctttgt atcttagact tgagcataaa aaacgctcgt taaaaacttt ttgaataaaa
    29161 aatatagaaa aggaactatt ttctcatgac aaaaggaatc ttagggaaaa aagtgggaat
    29221 gactcaaatc ttcactgaag ctggcgaatt gatccctgta acagttattg aagcaactcc
    29281 aaacgttgtt cttcaagtta aaactgttga aacagacgga tacaacgcta tccaagttgg
    29341 tttcgatgac aaacgcgaag tattgagcaa caaacctgct aaaggacatg tagcgaaagc
    29401 taacacggct cctaagcgct tcattcgtga attcaaaaac gttgaaggct tggaagttgg
    29461 tgctgaaatt acagttgaaa cattcgcagc tggagacgtt gttgacgtaa cgggtacttc
    29521 taaaggtaaa ggtttccaag gtgttatcaa acgccacgga caatcacgtg gaccaatggc
    29581 tcacggttct cgttaccacc gtcgtccagg ttctatgggg cctgttgcac ctaaccgcgt
    29641 attcaaaggt aaaaaccttg caggacgtat gggtggcgac cgcgtaacaa ttcaaaacct
    29701 tgaagttgta caagttgttc cagaaaagaa cgttatcctt atcaaaggta acgtaccagg
    29761 tgctaagaaa tctcttatca ctatcaaatc agcagttaaa gctggtaaat aataaagaaa
    29821 ggggaaatca gtcacaatgg caaacgtaac attatttgac caaactggta aagaagctgg
    29881 ccaagttgtt cttagcgatg cagtatttgg tatcgaacca aatgaatcag ttgtgtttga
    29941 tgtaatcatc agccaacgcg caagccttcg tcaaggaaca cacgctgtta aaaaccgctc
    30001 tgcagtatca ggtggtggac gcaaaccatg gcgtcaaaaa ggaactggac gtgctcgtca
    30061 aggttctatc cgctcaccac aatggcgtgg tggtggtgtt gtcttcggac caactccacg
    30121 ttcatacggc tacaaacttc cacaaaaagt tcgtcgccta gctcttaaat cagtttactc
    30181 tgaaaaagtt gctgaaaaca aattcgtagc tgtagacgct ctttcattta cagctccaaa
    30241 aactgctgaa tttgcaaaag ttcttgcagc attgagcatc gattctaaag ttcttgttat
    30301 ccttgaagaa ggaaatgaat tcgcagctct ttcagctcgt aaccttccaa acgtgaaagt
    30361 tgcaactgct acaactgcaa gtgttcttga catcgcaaat agcgacaaac ttcttgtcac
    30421 acaagcagct atctctaaaa tcgaggaggt tcttgcataa tgaatttgta tgatgttatc
    30481 aaaaaacctg tcatcactga aagctcaatg gctcaacttg aagcaggaaa atatgtattt
    30541 gaagttgaca ctcgtgcaca caaacttttg atcaagcaag ctgttgaagc tgctttcgaa
    30601 ggtgttaaag ttgccaatgt taacacaatc aacgtaaaac caaaagctaa acgtgttgga
    30661 cgttacactg gttttactaa caaaactaaa aaagctatca tcacacttac agctgattct
    30721 aaagcaatcg agttgtttgc tgctgaagct gaataatcta aggaggaaat atcgtgggaa
    30781 ttcgtgttta taaaccaaca acaaacggtc gccgtaatat gacttctttg gatttcgctg
    30841 aaatcacaac aagcactcct gaaaaatcat tgcttgttgc attgaagagc aaggctggtc
    30901 gtaacaacaa cggtcgtatc acagttcgtc accaaggtgg tggacacaaa cgtttctacc
    30961 gtttggttga cttcaaacgt aataaagaca acgttgaagc agttgttaaa acaatcgagt
    31021 acgatccaaa ccgttctgca aacatcgctc ttgtacacta cactgacggt gtgaaagcat
    31081 acatcatcgc tccaaaaggt cttgaagtag gtcaacgtat cgtttcaggt ccagaagcag
    31141 atatcaaagt cggaaacgct cttccacttg ctaacatccc agttggtact ttgattcaca
    31201 acatcgagtt gaaaccaggt cgtggtggtg aattggtacg tgctgctggt gcatctgctc
    31261 aagtattggg ttctgaaggt aaatatgttc ttgttcgtct tcaatcaggt gaagttcgta
    31321 tgattcttgg aacttgccgt gctacagttg gtgttgtcgg aaacgaacaa catggacttg
    31381 taaaccttgg taaagcagga cgtagccgtt ggaaaggtat ccgcccaaca gttcgtggtt
    31441 ctgtaatgaa ccctaacgat cacccacacg gtggtggtga aggtaaagca ccagttggtc
    31501 gtaaagcacc atctactcca tggggcaaac ctgctcttgg tcttaaaact cgtaacaaga
    31561 aagcgaaatc tgacaaactt atcgttcgtc gtcgcaacga gaaataatat taaactagtc
    31621 gcttaagcaa ctagtaaatc cgccagctcg gtagcgctcc ataggagtgc aagccgctgt
    31681 ggtacaacat ttaaaggaga aaatataaaa atgggacgca gtcttaaaaa aggacctttc
    31741 gtcgatgagc atttgatgaa aaaagttgaa gctcaagcta acgacgaaaa gaaaaaagtt
    31801 attaaaactt ggtcacgtcg ttcaacgatc ttcccaagtt tcattggtta cactattgca
    31861 gtttatgacg gacgtaaaca cgtacctgtt tacatccaag aagacatggt aggccacaaa
    31921 cttggtgaat ttgcaccaac tcgtacttac aaaggtcacg ctgcagacga caagaaaaca
    31981 cgtagaaaat aaggagaaca taaatggcag aaattacttc agctaaagca atggctcgta
    32041 cagtacgtgt ttcacctcgt aaatcacgtc ttgttcttga taacatccgt ggtaaaagcg
    32101 tagccgatgc aatcgcaacc ttgacattca ctccaaacaa agctgctgaa atcatcttga
    32161 aagttttgaa ctcagctgta gctaacgctg aaaacaactt tggtttggat aaagctaact
    32221 tggtagtatc tgaagcattc gcaaacgaag gaccaactat gaaacgtttc cgtccacgtg
    32281 cgaaaggttc agcttcacca atcaacaaac gtacagctca catcactgta gctgttgcag
    32341 aaaaataagg aggtaaaatc gtgggtcaaa aagtacatcc aattggtatg cgtgtcggca
    32401 tcatccgtga ttgggatgcc aaatggtatg ctgaaaaaga atacgcggat taccttcatg
    32461 aagatcttgc aatccgtaaa ttcgttcaaa aagaacttgc tgacgcagca gtttcaacta
    32521 ttgaaatcga acgcgcagta aacaaagtta acgtttcact tcacactgct aaaccaggta
    32581 tggttatcgg taaaggtggt gctaacgttg atgcactccg tgcaaaactt aacaaattga
    32641 ctggaaaaca agtacacatc aacatcatcg aaatcaaaca acctgatttg gatgctcacc
    32701 ttgtaggtga aggaattgct cgtcaattgg agcaacgtgt tgctttccgt cgtgcacaaa
    32761 aacaagcaat ccaacgtgca atgcgtgctg gagctaaagg aatcaaaact caagtatcag
    32821 gtcgtttgaa cggtgcagat atcgcccgtg ctgaaggata ctctgaagga actgttccgc
    32881 ttcacacact tcgtgcagat atcgattacg cttgggaaga agcagatact acatacggta
    32941 aacttggtgt taaagtatgg atctaccgtg gtgaagttct tccagctcgt aaaaacacta
    33001 aaggaggtaa ataaccaatg ttagtaccta aacgtgttaa acaccgtcgt gagttccgtg
    33061 gaaaaatgcg cggtgaagca aaaggtggaa aagaagtagc attcggtgaa tacggtcttc
    33121 aagctacaac tagccactgg atcactaacc gccaaatcga agctgctcgt atcgccatga
    33181 ctcgttacat gaaacgtggt ggtaaagttt ggattaaaat cttcccacac aaatcataca
    33241 ctgctaaagc tatcggtgtg cgtatgggat ctggtaaagg ggcacctgaa ggttgggtag
    33301 caccagttaa acgtggtaaa gtgatgttcg aaatcgctgg tgtatctgaa gagattgcac
    33361 gtgaagcgct tcgacttgct agccacaaat tgccagttaa atgtaaattc gtaaaacgtg
    33421 aagcagaata aggagaaggc atgaaactta atgaagtaaa agaatttgtt aaagaacttc
    33481 gtggtctttc tcaagaagaa ctcgcgaagc gcgaaaacga attgaaaaaa gaattgtttg
    33541 aacttcgttt ccaagctgct actggtcaat tggaacaaac agctcgcttg aaagaagtta
    33601 aaaaacaaat cgctcgcatc aaaacagttc aatctgaagc gaaataatag actagggaag
    33661 gagaaatttc aatggaacgc aataatcgta aagttcttgt tggacgtgtt gtatctgaca
    33721 aaatggacaa gacaatcaca gttgtagttg aaacaaaacg taaccaccca gtctatggta
    33781 aacgtattaa ctactctaaa aaatacaaag ctcatgatga aaacaatgtt gccaaagaag
    33841 gcgatatcgt acgtatcatg gaaactcgcc cgctttcagc tacaaaacgt ttccgtcttg
    33901 tagaagttgt tgaagaagcg gtcatcatct aatcaaacct gaaaggagaa aactgaaatg
    33961 attcaaacag aaactcgttt gaaagtcgca gacaacagcg gtgctcgcga aatcttgact
    34021 atcaaagttc ttggtggttc aggacgtaaa tttgcaaaca tcggtgatgt tatcgtggca
    34081 tctgtaaaac aagctactcc tggtggtgcg gttaaaaaag gtgacgttgt taaagcagtt
    34141 atcgttcgta ctaaatcagg tgctcgtcgt gctgatggtt catacatcaa atttgacgaa
    34201 aacgcagcag ttatcatccg tgaagacaaa actcctcgcg gaacacgtat ctttggccca
    34261 gttgcacgtg aattgcgtga aggtggcttc atgaagatcg tgtcacttgc tccagaagta
    34321 ctttaatttt taggaacaaa ctagtcccct agcttcaagc tagggtgccc ttatgggcgt
    34381 aagaaaaatc aaggagaaac ctaatgtttg taaaaaaagg cgacaaagtt cgcgtaatcg
    34441 ctggtaaaga taagggaaca gaagctgttg tccttactgc ccttccaaaa gtaaacaaag
    34501 ttatcgttga aggtgttaac attgttaaga aacaccaacg tccaactaac gagcttcctc
    34561 aaggtggtat catcgagaaa gaagcagcta tccacgtatc aaacgttcaa gttttggaca
    34621 aaaatggtgt agctggtcgt gttggataca aatttgtaga cggtaaaaaa gttcgctaca
    34681 acaaaaaatc aggcgaagtg cttgattaat cacgaaggaa aggagaagta taatggcaaa
    34741 tcgtttaaaa gaaaaatatc ttaatgaagt agttcctgct ttgacagaac aattcaacta
    34801 ctcatcagtg atggctgtgc ctaaagtaga taagattgtt ttgaacatgg gtgttggtga
    34861 agctgtatca aacgctaaaa gccttgaaaa agctgctgaa gaattggcac ttatctcagg
    34921 tcaaaaacca cttatcacta aagctaaaaa atcaatcgcc ggcttccgtc ttcgtgaagg
    34981 tgttgcgatc ggtgcaaaag ttacccttcg tggtgaacgt atgtacgaat tcttggataa
    35041 attggtatca gtttcacttc cacgtgtacg tgacttccac ggtgtcccaa caaaatcatt
    35101 tgatggacgc gggaactaca cacttggtgt gaaagaacaa ttaatcttcc cagaaatcaa
    35161 cttcgatgac gttgacaaaa ctcgtggtct tgacatcgtt atcgtaacaa ctgctaacac
    35221 tgacgaagag tcacgtgcat tgcttacagg ccttggaatg ccttttgcaa aataatatag
    35281 gaggtaaatc taatggctaa aaaatcaatg gtagctagag aggctaaacg ccaaaaaatt
    35341 gttgaccgtt atgctgaaaa acgtgctgca ttaaaggcgg caggggacta cgaaggttta
    35401 tctaaattac ctcgcaacgc ctcaccgact cgtttacata atcgttgtag ggttacgggg
    35461 cgcccacatt cagtttaccg caaatttggt ctgagtcgta tcgcttttcg cgaacttgcg
    35521 cataaaggtc aaattcctgg tgtaacaaaa gcatcttggt aatttaagat atcaagagcg
    35581 tcaaaactcc aagtaaaaat aggaaacttg acgaagaaac taaagtttct aggaaagttt
    35641 atctttttca cacagagttt agcccgggtt caattgggct tgccaatttg aacacgagct
    35701 acagctttgg caaaaaagac caatttgctt tggagcattg cttctgcatt aaattgtcta
    35761 tttttgctcg tgctgttacg ctctttgtat catgtattaa ctagcaagtg caacttgcaa
    35821 actactagta agaggagaaa aacaaaatgg ttatgactga cccaatcgca gacttcctaa
    35881 ctcgtattcg taatgctaac caagctaaac acgaagtact tgaagtacct gcatcaaaca
    35941 tcaaaaaagg gattgctgaa atccttaaac gcgaaggttt tgtaaaaaac gttgaaatca
    36001 ttgaagatga caaacaaggc gtcatccgtg tatttcttaa atacggacca aatggtgaga
    36061 aagttatcac taacttgaaa cgtgtttcta aaccaggact tcgtgtctac aaaaaacgtg
    36121 aagaccttcc aaaagttctt aacggacttg gaattgccat cctttcaact tctgaaggtt
    36181 tgcttactga taaagaagca cgccaaaaga atgttggtgg tgaggttatc gcttacgttt
    36241 ggtaaaatca agatacaaag ctcgtaaaga acaaagcaaa attaggaagt tggagaagtt
    36301 tgtttacaaa caagccaact tatctatttt gcacagttct tagagcgtgt tcagttcagc
    36361 tcttgaacta aataagtatc tgaaccccgt gaaaactggc cgttctggcc tgacaattta
    36421 acaggagaaa ataaacatgt cacgtattgg taataaagtt atcgtgttgc ctgctggtgt
    36481 tgaactcgct aacaatgaca acgttgtaac tgtaaaagga tctaaaggag aacttactcg
    36541 tgagttctca aaagatattg aaatccgtgt ggaaggtact gaaataactc ttcaccgtcc
    36601 aaacgattca aaagaaatga aaactatcca cggaactact cgtgcccttt tgaacaacat
    36661 ggttgttggt gtatcagaag gattcaagaa agaacttgaa atgcgtgggg ttggttaccg
    36721 tgcacagctt caaggatcta aacttgtttt ggctgttggt aaatctcatc cagacgaagt
    36781 tgaagctcca gaaggaatta cttttgaact tccaaaccca acaacaatcg ttgttagcgg
    36841 aatttcaaaa gaagtagttg gtcaaacagc tgcttacgta cgtagccttc gttcaccaga
    36901 accatataaa ggtaaaggta tccgttacgt tggtgaattc gttcgccgta aagaaggtaa
    36961 aacaggtaaa taatgttgag tggttgatca tcaaccacca acctattttc caactttgtg
    37021 catagcacac gatttaaaac taaagaggtg aaaactgtga tttcaaaacc agataaaaac
    37081 aaactccgcc aaaaacgcca ccgtcgcgtt cgcggaaaac tctctggaac tgctgatcgc
    37141 ccacgtttga acgtattccg ttctaataca ggcatctacg ctcaagtgat tgatgacgta
    37201 gcgggtgtaa cgctcgcaag tgcttcaact cttgataaag aagtttcaaa aggaactaaa
    37261 actgaacaag ccgttgctgt cggtaaactc gttgcagaac gtgcaaacgc taaaggtatt
    37321 tcagaagtgg tgttcgaccg cggtggatat ctatatcacg gacgtgtgaa agctttggct
    37381 gatgcagctc gtgaaaacgg attgaaattc taataggagg acactagaaa atggcattta
    37441 aagacaatgc agttgaatta gaagaacgcg tagttgctgt caaccgtgtt acaaaagttg
    37501 ttaaaggtgg acgtcgtctt cgtttcgcag ctcttgttgt tgttggtgac cacaatggtc
    37561 gcgtaggatt tggtactggt aaagctcaag aagttccaga agcaatccgt aaagcagtag
    37621 atgatgctaa gaaaaacttg atcgaagttc ctatggttgg aacaacaatc ccacacgaag
    37681 ttctttcaga attcggtgga gctaaagtat tgttgaaacc tgctgtagaa ggttctggag
    37741 ttgccgctgg tggtgcagtt cgtgccgttg tggaattggc aggtgtggca gatattacat
    37801 ctaaatcact tggttctaac actccaatca acattgttcg tgcaactgtt gaaggtttga
    37861 aacaattgaa acgcgctgaa gaaattgctg cccttcgtgg tatttcagtt tctgatttgg
    37921 cataagaaag gggataaaat ggctcaaatt aaaattactt tgactaagtc tccaatcgga
    37981 cgcattccat cacaacgtaa aactgttgta gcacttggac ttggcaaatt gaacagctct
    38041 gttattaaag aagataacgc tgctatccgt ggtatgatca cagcagtatc tcacttagta
    38101 acagttgaag aagtaaacta atgaagtttt aggggatgtg cactgtacca tcccctaaaa
    38161 ctagatatag tcatctatga tgacatcgta taggcgagtt gatgggggag acaacctttt
    38221 ctcccttatc ggcgctagca ttttacaaaa gaggagaaaa taaaaatgaa acttcatgaa
    38281 ttgaaacctg cagaaggttc tcgtaaagta cgtaaccgcg ttggtcgtgg tacttcatca
    38341 ggtaacggta aaacatctgg tcgtggtcaa aaaggtcaaa aagctcgtag cggtggcgga
    38401 gttcgccttg gttttgaagg tggacaaact ccattgttcc gtcgtcttcc aaaacgtgga
    38461 ttcactaaca tcaacgctaa agaatacgca attgtgaacc ttgaccaatt gaacgtcttt
    38521 gaagatggtg ctgaagtaac tccagttgtt cttatcgaag caggaattgt taaagctgaa
    38581 aagtcaggta ttaaaattct tggtaacggt gagttgacta agaaattgac tgtgaaagca
    38641 gctaaattct ctaaatcagc tgaagaagct atcactgcta aaggtggttc agtagaagtc
    38701 atctaagaga ggtgacctat gttttttaaa ttattaagag aagctcttaa agtcaagcag
    38761 gttcgatcaa aaattttatt tacaattttt atcgttttgg tctttcgtat cggaactagc
    38821 attacagttc ctggtgtgaa tgccaatagc ttgaatgctt taagtggatt atccttctta
    38881 aacatgttga gcttggtgtc ggggaatgcc ctaaaaaact tttcgatttt tgccctagga
    38941 gttagtccct atatcaccgc ttctattgtt gtccaactct tgcaaatgga tattttaccc
    39001 aagtttgtag agtggggtaa acaaggggaa gtaggtcgaa gaaaattgaa tcaagctact
    39061 cgttatattg ctctagttct cgcttttgtg caatctatcg ggattacagc tggttttaat
    39121 accttggctg gagctcaatt gattaaaact gctttaactc cacaagtttt tctgacgatt
    39181 ggtatcatct taacagctgg tagtatgatt gtcacttggt tgggtgagca aattacagat
    39241 aagggatacg gaaacggtgt ttccatgatt atctttgccg ggattgtttc ctcaattcca
    39301 gagatgattc agggcatcta tgtggactac tttgtgaacg tcccaagtag ccgtatcact
    39361 tcatctatca ttttcgtaat cattttgatt attactgtat tgttgattat ttactttaca
    39421 acttatgttc aacaagcaga atacaaaatt ccaatccaat atactaaggt tgcacaaggt
    39481 gctccatcta gctcttacct tccgttaaaa gtaaaccctg ctggagttat ccctgttatc
    39541 tttgccagtt cgattactgc agcgcctgcg gctattcttc agtttttgag tgccacaggt
    39601 catgattggg cttgggtaag ggtagcacaa gagatgttgg caactacttc tccaactggt
    39661 attgccatgt atgctttgtt gattattctc tttacattct tctatacgtt tgtacagatt
    39721 aatcctgaaa aagcagcaga gagcctacaa aagagtggtg cctatatcca tggagttcgt
    39781 cctggtaaag gtacagaaga atatatgtct aaacttcttc gtcgtcttgc aactgttggt
    39841 tccctcttcc ttggtgtgat ttccatttta ccgattgcag ctaaagatgt atttggtctt
    39901 tctgatgttg ttgcctttgg tggaacaagt ctcttgatca ttatctctac aggtatcgaa
    39961 ggaatcaagc aattggaagg ttacctattg aaacgtaagt atgttggttt catggacaga
    40021 acagaataaa agtatttact gaatcagtaa atactgaggg agtggaggtt taaactctga
    40081 catttgtaag agttggatct cccctcttct attttgtttt taaatcgggg tgaaaagact
    40141 ttttgcttct atttaaaaat aaaataagga gatcaaatca tgaatctttt gattatgggc
    40201 ttacctggtg caggtaaggg aactcaagca gcaaaaatcg tagaacaatt ccatgttgca
    40261 catatctcaa caggtgatat gttccgcgct gcaatggcaa atcaaactga aatgggtgtt
    40321 cttgctaagt catatattga caagggtgaa ttggttcctg acgaagttac aaatggaatc
    40381 gtaaaagaac gcctttcaca agatgatatt aaagaaacag gattcttatt ggatggttac
    40441 ccacgtacaa ttgaacaagc tcatgccttg gacaaaacat tggctgaact tggcattgaa
    40501 ctagaaggtg ttatcaatat tgaagtgaac cctgacagcc ttttggaacg tttgagtggg
    40561 cgtatcatcc accgcgtaac tggagaaact ttccacaagg tctttaaccc accagttgac
    40621 tataaagaag aagattacta ccaacgtgaa gatgataagc ctgagacagt aaaacgtcgt
    40681 ttggatgtta atattgctca aggagaacca atcattgctc actaccgtgc caaaggtttg
    40741 gttcatgaca tcgaaggtaa tcaagatatc aatgatgtct tctcagatat tgaaaaagta
    40801 ttgacaaatt tgaaataaag cgtttttcac acttgcaaaa atccgctaca aatgttatac
    40861 tgagatagtc tgacttataa ttgttgtctc tgtgtctaga ggcatcgaat cgaaatttat
    40921 ggaggtgctt ttgcgtggca aaagacgatg tgattgaagt tgaaggcaaa gtagttgata
    40981 caatgccgaa tgcaatgttt acggttgaac ttgaaaatgg acatcagatt ttagcaacag
    41041 tttctggtaa aattcgtaaa aactatattc gtattttagc gggagatcgt gttactgtcg
    41101 aaatgagtcc atatgacttg acacgtggac gtatcactta ccgctttaaa taatcgaaaa
    41161 acttggaggg ataagaaatg aaagtaagac catcggtcaa accaatttgc gaatactgta
    41221 aagttattcg tcgtaatggt cgtgttatgg taatttgccc agcaaatcca aaacacaaac
    41281 aacgtcaagg ataagataga aaggagaaaa catggctcgt attgctggag ttgatattcc
    41341 aaatgacaaa cgcgtagtaa tctcattgac ttatgtttat ggtatcggac ttgcaacatc
    41401 taagaaaatt ttggctgctg ctggaatctc agaagatgtt cgtgtacgtg atcttacatc
    41461 agatcaagaa gatgctatcc gtcgtgaagt ggatgcaatc aaagttgaag gtgaccttcg
    41521 tcgtgaagta aacttgaaca tcaaacgttt gatggaaatc ggttcatacc gtggtatccg
    41581 tcaccgtcgt ggacttcctg tccgtggaca aaacactaaa aacaacgccc gcactcgtaa
    41641 aggtaaagct gttgcgattg ctggtaagaa aaaataatat aggaggtaaa agtcttggct
    41701 aaaccaacac gtaaacgtcg tgtgaaaaag aatatcgaat ctggtattgc tcatattcac
    41761 gctacattta ataacactat tgttatgatt actgatgtgc atggtaatgc aattgcttgg
    41821 tcatcagctg gtgctcttgg tttcaaaggt tctcgtaaat ctacaccatt cgctgctcaa
    41881 atggcttctg aagctgctgc taaatctgca caagaacacg gtcttaaatc agttgaagtt
    41941 actgtaaaag gtccaggttc tggtcgtgag tcagctattc gtgcgcttgc tgccgctggt
    42001 cttgaagtaa cagcaattcg tgatgtgact ccagtgccac acaatggtgc tcgtcctcca
    42061 aaacgtcgcc gtgtataatc atcgcattac actgcttttc gtttaagagg gagtaactaa
    42121 atgatcgagt ttgaaaaacc aaatataaca aaaattgatg aaaataaaga ttatggcaag
    42181 tttgtaatcg aaccacttga acgtggctac ggtacaactc ttggtaactc tcttcgtcgt
    42241 gtacttctag cttctctacc aggagcagct gtgacatcta tcaacattga tggtgtgtta
    42301 catgagtttg acacagttcc aggtgttcgt gaagacgtga tgcaaatcat tctgaacatt
    42361 aaaggaattg cagtgaaatc gtacgttgaa gacgaaaaaa tcatcgaact ggatgttgaa
    42421 ggtcctgctg aagtaacagc tggtgacatt ttgacagata gcgatattga aattgtaaat
    42481 ccagatcatt atctctttac aatcggtgaa ggttcttctc taaaagcgac tatgactgtt
    42541 aacagtggtc gtggatatgt acctgctgat gaaaataaaa aggataatgc accagttgga
    42601 acacttgctg tagattctat ttatacacca gttacaaaag tcaactatca agtggaacct
    42661 gctcgtgtag gtagcaatga tggtttcgac aaattaaccc ttgaaatctt gacaaatgga
    42721 acaattattc cagaagatgc tttagggctt tcagcacgta ttttgacaga acatcttgat
    42781 ttgtttacaa atcttactga gattgctaag tcaactgaag tgatgaaaga agctgatact
    42841 gaatctgacg accgtatttt agatcgtacg attgaggaac tggacttgtc tgtgcgttca
    42901 tacaactgtt taaaacgtgc cggtatcaat actgtgcatg atttgacaga aaaatctgaa
    42961 gcagagatga tgaaagtacg aaatcttgga cgcaagagtt tggaagaagt gaaactcaaa
    43021 ctcattgatt tgggtcttgg attaaaagat aaataaagga ggaatacatg gcttaccgta
    43081 aactaggacg cactagctca caacgtaaag caatgcttcg cgatttgaca actgaccttt
    43141 tgatcaacga atcaatcgtg acaactgaag ctcgtgctaa agaaatccgt aaaactgttg
    43201 aaaaaatgat tactctaggt aaacgtggtg atttgcatgc acgtcgtcaa gcagctgctt
    43261 tcgtacgtaa tgaaatcgca tctgaaaact atgatgaagc aactgataag tacacttcta
    43321 ctacagcact tcaaaaattg ttctcagaaa tcgcacctcg ttatgctgaa cgtaacggtg
    43381 gatacactcg tatccttaaa actgaatcac gtcgtggtga tgcagcgcca atggcgatca
    43441 tcgaattagt ataaaatcat caattttgtt gagtgttatg atgatggagt cttgtgctct
    43501 tagtctagct ctggtctacc gctaggattt cggtcctagc gggaacactc atcataagtt
    43561 gggatagtag acgcttgttt acgaaattgt ttttttctta agaacaactt cgtaagcagg
    43621 cgtttttgag tattttcgtt agaattatgc tatactattt gaaaagaatc ctgtttaatg
    43681 ttaaggtttc ttattttaag aagaattgga gtttacttat gaaagccatt ataactgttg
    43741 ttggtaaaga taaatctgga attgttgcag gtgtttctgg taaaattgca gaattaggat
    43801 tgaatattga cgatatctct caaactgtct tggatgaata ttttacgatg atggctgttg
    43861 tatctagtga tgaaaagcaa gattttacct atcttcgtaa tgaatttgaa gcttttgggc
    43921 aaactttgaa tgtaaaaatc aatattcaga gtgcagcgat tttcgaagct atgtataata
    43981 tctaggaggt catcatggat attagacaag ttactgaaac catcgccatg attgaggagc
    44041 aaaacttcga tattagaacc attaccatgg ggatttctct tttggactgt atcgatccag
    44101 atatcaatcg tgctgcggag aaaatctatc aaaaaattac gacaaaggcg gctaatttag
    44161 tagctgttgg tgatgaaatt gcggctgagt tgggaattcc tatcgttaat aagcgtgtat
    44221 cggtgacacc tatttctctg attggggcag cgacagatgc gacggactac gtggttctgg
    44281 caaaagcgct tgataaggct gcgaaagaga ttggtgtgga ctttattggt ggtttttctg
    44341 ccttagtaca aaaaggttat caaaagggag atgagattct catcaattcc attcctcgcg
    44401 ctttggctga gacggataag gtctgctcgt cagtcaatat cggctcaacc aagtctggta
    44461 ttaatatgac ggctgtggca gatatgggac gaattatcaa ggaaacagca aatctttcag
    44521 atatgggagt ggccaagttg gttgtattcg ctaatgctgt tgaggacaat ccatttatgg
    44581 cgggtgcctt tcatggtgtt ggggaagcag atgttatcat caatgtcgga gtttctggtc
    44641 ctggtgttgt gaaacgtgct ttggaaaaag ttcgtggaca gagctttgat gtagtagccg
    44701 aaacagttaa gaaaactgcc tttaaaatca ctcgtatcgg tcaattggtt ggtcaaatgg
    44761 ccagtgagag actgggtgtg gagtttggta ttgtggactt gagtttggca ccaacccctg
    44821 cggttggaga ctctgtggca cgtgtccttg aggaaatggg gctagaaaca gttggcacgc
    44881 atggaacgac ggctgccttg gccctcttga acgaccaagt taaaaagggt ggagtgatgg
    44941 cctgcaacca agtcggtggt ttatctggtg cctttatccc tgtttctgag gatgaaggaa
    45001 tgattgctgc agtgcaaaat ggctctctta atttagaaaa actagaagct atgacggcta
    45061 tctgttctgt tggattggat atgattgcca tcccagaaga tacgcctgct gaaactattg
    45121 cggctatgat tgcggatgaa gcagcaatcg gtgttatcaa catgaaaaca acagctgttc
    45181 gtatcattcc caaaggaaaa gaaggcgata tgattgagtt tggtggtcta ttaggaactg
    45241 cacccgttat gaaggttaat ggggcttcgt ctgtcgactt catctctcgc ggtggacaaa
    45301 tcccagcacc aattcatagt tttaaaaatt aagaaaatag gagaaatttt aagttctatt
    45361 taagattaga cgtgtatact ataatcatta aataaagacc tcctaatatt atttgaaaca
    45421 gataacactg aattagtttg aatttgattt tcatctaata tctttattta atgaactcct
    45481 aaactttttc ataataatct ccttcaaaag tcgcctgtat gggtggcttt tattttatca
    45541 ttcatgatat aatagaagca aacggaggac ggaaaatggt aaaagtacga ttgtatttgg
    45601 tacgtcatgg caagaccatg tttaacacga ttggtcgcgc gcaaggttgg agcgatactc
    45661 ccttaactgc tgaaggtgaa cgagggattc aagagttagg aatcggtttg cgagaatctg
    45721 atctacagtt tgagcgtgct tattcgagtg attctggtcg taccattcag accatgggaa
    45781 ttatccttga agaacttggc ttgcaggggg aaatccctta tcgcatggac aagcgtatca
    45841 gagaatggtg tttcggtagt tttgatggag cctatgatgg cgatcttttc atgggcatta
    45901 ttcctcgtat ctttaatgtg gaccacgttc accaattgtc ttatgctgaa ctggctgagg
    45961 gcttggtaga ggtcgataca gctggttggg ctgaaggctg ggaaaaactc agtggccgaa
    46021 tcaaggaagg ctttgaaatg attgcaaaag aaatggaaga tcaaggtgga ggtaacgccc
    46081 ttgttgtcag ccatggaatg actattggaa ccattgttta tctgattaat ggcatgcatc
    46141 cgcatggtct ggataatggt agcgtgacaa tccttgaata tgaggacggc cagtttaggg
    46201 ttgaagttgt cggtgaccgt agttaccgag agctaggacg tgagaagatg gaagaaggct
    46261 ctatttaatc agtctagact tgcttgccat gagctaggga tttgataaga atatcaagat
    46321 aagaaaaaac agccgagggc actcctttcg gctgtttttg atgtggaaaa ctaaagtgta
    46381 atgctattgc ttttagagat tttcataaac aagagcaagg aacctactgt tagaacagtc
    46441 aggatagttg acaaggttgc ggctacaccg taatttcctc tgagaacctc tgtataaata
    46501 gctacagtca ttgttcttgt tttgacattg tagaggagga tagaagtaga gagttttgaa
    46561 atcattgtga ctcaagataa gatggctcca gaaatgatac cagatagcat cattggagtt
    46621 gtaatcttag caaaggtatt gagacgacta cttcctaagc tttcagcagc ttcttcaata
    46681 cttggtgcta tttgttgtaa gctagcaaca gatgagcgaa tagtataagg taatcttctg
    46741 gcagatagag acataatcaa gatgaaagca gtccctgtaa tcataagaaa tccacttcca
    46801 aatagaccag tattgaagga agaaatgaag gcaatcccta gaacggttcc tggtacaata
    46861 taaggtacca tactgaggct gtcaattaag tttgtaaaca aattccgttt tctaacggct
    46921 aggtaggaga taaatgtcgc aaatagaaca actagaacta aggcaatcaa agggatacga
    46981 atggtattga aaatagcaga tcccatacga tggaaagcta ccttgtaact gtttggagaa
    47041 taacctttaa cagataccat acctgatgtt tttaggaaag aggtataaat taagtagatt
    47101 tgaggtaaaa cagagataaa gataattccg tagactgttg cataaatggc agccattttt
    47161 ccttttgtag tttttttagg ctcaattgga tggagcagat tcatgctgaa actgtagcgg
    47221 tttgcaatgt gtttttggat aaggaaaatt gccaaggcaa tgataatcgc cataattgca
    47281 aaagcagaat ttcctccaac ctcgctaata aattgggtat aaatcaggac agggaaagtc
    47341 cgataccctt cgccaatcaa cataggcgtt ccaaagtctg agaatgctct cataaataca
    47401 agcaaggagc tgctagtaag gttggaacta ggagaggtaa aacaaccgtt acgataggtt
    47461 taaatccgaa ggaccccatg ctttcagctg cttcaagtag agaattgtca atactgttca
    47521 ttgttccagc aacatataga aataccagtg ggaatagttg cagtgtaaag acaagtacaa
    47581 ttcctttgaa tcaataaata tcgatagctg gaagataaag ggcatttgtc aaaaatttag
    47641 tgatgacctc atttcgtcct agcaagagaa cccaggagta ggctcctacg aaaggagctg
    47701 acatggaagc aatgataatc aatatttgta gaaatttctt ccccttgaag tcatacatag
    47761 agaagagata agctaatagg gttcctacaa ctaaggaagt gatagtagcg gtaatggaaa
    47821 ccttgaaact gttgactagt gtctcagagt agtaggcttt actaaagaaa gtgacaaaat
    47881 tagctagtga gaattgtcct tcatgtataa gtgcttgctt gagcacggta acgataggat
    47941 aaacgagaaa gataggatag gtaagaaaga ggaagaaaga ggaaactgtc caaatattta
    48001 gttttttacg ttccatggtt gactcctttt atcaggtttt gggaaccatc tgcagaaaag
    48061 atgtttaatt tttgcgtatt gattcgtaga cgaatacgat tgcctttttg tagatcttct
    48121 tcaaaagttg attcttcact aacttgaatt tttgaggcaa aacctgtctc aatgaaataa
    48181 tccgtattta gtccaagata gacgctatct ctaatagttc cttcaatatc tccagattca
    48241 tctttgataa actcttcggg acgaatgctt acatgaatag cttgctcctc aacctgatca
    48301 agagctggca ttcgaagggc atagccatct gaaaagacga tataagcgcc gtcgctccgt
    48361 ttttcaagat tggcagggat aatatttgtg cgtccgataa aggttgccac aaactcatta
    48421 gctggtttat gatagagttc ttttggtcgg ccgatttgtt ggatcacccc atctttcata
    48481 acagcaattt ggtctgaaat agccatggct tcttcttggt cgtgggttac ataaacagtt
    48541 gtaattccca cttcgtgttg gatttctcgg atggcttgac gcatatccaa gcgaagtttg
    48601 gcctccagat tactaagtgg ctcgtccatg aggagaacac ttggattaac cgctaaggcg
    48661 catgccaagg tgacacgttg ttgttgtcca ccactgagtt tatcgggctt tcgatccgca
    48721 tattgagcaa tttgcatgag ttcaagatac ttgttggtct gttgaatcaa ttcttctttt
    48781 ggaaccttct tttgcataag accaaaagca acgttgtctc ggacagtcaa atgtgggaaa
    48841 atagcgtagt tttggaaaac catcccgata ttgcgtttgc tgggttccat attattgatt
    48901 tttgtatcat cgaagtaaaa ttctccacct tcgatactgt tgaaacctgc aatcatacga
    48961 agaagggtcg ttttcccaca tcctgaagct ccaagaaggg taaagagact tccttttgga
    49021 attgtaatgt tcaaattctc aataacaggg acatcgtggt agattttttt ggcgttaata
    49081 attttgatct cactcatagt gaacctcttt tactgtttag attggatatc tgtaaagact
    49141 tcgttgtatt tcttaacgat atctgattta ttcttgatga cataatcata atcttcagtg
    49201 agtgttttga ttttgtcaat tggtttcatg ttttcgcttg ttttagcatt tttacgaaca
    49261 ggacggttag tagtggttgt accaagtgta tcttgtactt cttgagagat aataaaatcg
    49321 ataaatttct tggcattttc catattttta gattttttaa cgatagcagc actagcaggt
    49381 aggaagacgg ttccttcttt tggatagact accttaatgt tagctccgtc atttaagagt
    49441 ttaactgctg gatcttcata agagagacca acagccattt ctccatcagc gactacttta
    49501 tagacactag atgaacttga accgatttta ccatcaataa gtgtgaaaag atcttttaca
    49561 taagaccaag ccttatcatc tttgtaacca ccttgagctt gtagcatatt tgttaattga
    49621 gcaaaggcgc tagaagagtt tgctgggtca gcagttgcga tttttccttt tagttcaggt
    49681 ttgaaaagat cgttatatcc ttcgatgttc atgcctttag ttaaatcagg gttgacgatt
    49741 aaaacactac catctagtgt ataaggagta gagtagccag ttgtgttttg atattctttg
    49801 ataacattat cattttcttt tgaagtatag ttttcaaaga gttctccgtg ggtagtatat
    49861 tgtgtataag aaccaccaaa gataacatca gctacaggaa cttctttttc tgactctagt
    49921 tttttgaaaa gttctccagt accagcttga atcagttcta ctttgatacc atatttttct
    49981 tcaaaggcag gaatagttgc tccaattaag ccctctgagt ttggtgaata aacgactagc
    50041 gaaccgccgt ctcctttatc agatgaactg tcatcggcag attcattaga agaacaagca
    50101 gcataataca tccatttctt tttcatgatg gatacctccg ttgtgttatt taagtttatt
    50161 ttaaaacaat gtaagcgttt ttaaaacata caattctatt ctatagtgta ttgaatctat
    50221 aacagtacac tttgactgct aaaatatttc tataaattaa tttgactttc ctgatagaga
    50281 tgttcacatc ttatttcaat tcactatatt agagtaaaat tctctacaaa aagaagaata
    50341 gcctatttta ctattcttct gagtgatttc aattcctttg gggaaatatg gagatacttt
    50401 ttaaatcctg acaaatggtt gtttcttttt ctaaatcggt gatactgtat cggagaatgc
    50461 gcgtgaggtc acaaaggctg cgatagagct tctatggaga atttcttttt ggagagattt
    50521 tttaaaggaa tgagacatcc gctacctcct tggaaggttt ttttgtgata agtatagcag
    50581 tttaaaaatt ctagagcaag gtaaaataga aaaggtttgg ctaaaatttt ttagctaaac
    50641 cttttgaaaa gttaagtatg tattatagat gcttgttatc tgcaaatagc tagagcattt
    50701 caaggaaata aactagaaat aacaattaat atggtggttc agaaatacct tttgataatc
    50761 aataagatct tcaggatgaa gtgcgttgat tccatccatt tcatatttcc gatttaataa
    50821 acgatatttg ttttcaccaa attgatgaaa agggagtagt tgaacttggt cgatatttaa
    50881 tgagttaaat agagtagcga atttttctgc atcctctaaa ctattgttaa aattaggaat
    50941 aactgggatt cttaaaacga tagttttatt ttgtgaaaaa gcataatgaa tgtttttaat
    51001 aatcatttga ttaaaaaccc cagtcacttt tttatgtttt atagaattat aatgttttag
    51061 gtctgtgtag ataaaatcca catattgaat taaatcaata aatttttcat gatcaacaaa
    51121 ggcagtagtt tcaatggcag tgtgtatgtg atgttcttta gctgatttta agatggcttt
    51181 agcaaattca aactgagcaa atatttcacc tcctgataaa gttaaacctc caccggattc
    51241 ttcgtaaaat tctttgtctt ttaatacctc tgtaataatt tcttctacag tcttttcttc
    51301 accgactaag gtgaatttct ctcgttgagc atctttcatt ttttcaggtt tcattctttg
    51361 agattcagga ttagaacacc atggacagcg cagaggacat ccttttaaaa aaacagttgt
    51421 acgaataccc ggaccgtcat gaattgaaaa gtgttgaata ttaaaaataa ttcctttaga
    51481 tatttccata agagttcctc ctcttcataa gtctatccta tcacaaaaac gaaagaaaaa
    51541 caattacgaa cgaaaggtta atttgttgtt tttttgcttt ttattttggt agaataaaac
    51601 ggaggtgtaa aatgaaaaga ttggaacaaa ttattaaatt agtatcagaa tatgaaaaga
    51661 tcgacgttaa tacattatcg gaaaaattaa atgtatcgaa agtaacgatt agaaaagatt
    51721 tagataaatt agagtcaaaa ggtttattac acagagagca tggatatgct gtattaaata
    51781 gtggagatga cttaaatgta cgtttgtcaa ttaattatga aactaagaga aaaattgttc
    51841 aggaagcagt aaaattggtg tcagataatg aaacaataat gatagaatct ggatcgacct
    51901 gtgctttact tgctgaggaa atttgcaagc aaaaaagaaa tgttacgatt gtaacaaatt
    51961 cgttttttat agcaaatttt gtgagagctt atgattcatg tcgtgttatt gttcttggtg
    52021 gtgagtttca gaaagattca caggtgactg taggaccttt attaaaagaa atgatacaga
    52081 cttttcatgt gtgtcaagct tttgttggga cagatggtta cgataaagag atgggcttta
    52141 ccggaaaaga tttaatgcgc agtgaggtag ttcaatatat ttcagcagtg tcggataaag
    52201 tcattgtcct aactgactca agtaaatttg ataaaagagg tacagtaaga agatttgctt
    52261 taagtcaagt ctatgaagta ataacagacg aaaaactttc taaacaaaat atagctacat
    52321 tagaaaatgc tgggataatg gttaaggtag tttcgtaaga ggttaagtgt atgaatcaag
    52381 ataggaataa actgctttct aaaattgctt atctgtatta tattgaaaac ttaaatcagt
    52441 cacaaatagc agcaaaatta ggaatttata gaacctctat tagtagaatg ttaacagaag
    52501 caaggaatgt aggaattgtt aaaattgaaa tagagaattt tgataccaat atgtttaagt
    52561 tggaaaatta tgtaaaagaa aaatacagtt tggaaagttt agaaattatt ccaaatgaat
    52621 ttgatgatac tccaacaatt ttatctgaaa gaatttctca agttgcagca ggcgtcctta
    52681 ggaatctaat tgatgataat atgaaaattg gcttttcttg ggggaaaagt ttaagtaatt
    52741 tagtagattt aattcacagt aaaagtgtcc gaaatgttca cttctatcct ctagcaggtg
    52801 gtcctagtca catacacgct aaataccatg tgaatacact gatttatgaa atgtctagaa
    52861 aatttcatgg agagtgtaca tttatgaatg caacgattgt gcaagaaaat aaattgttag
    52921 cagatggtat tttgcaatca agatattttg aaaatttgaa aaatagttgg aaagatttag
    52981 atatagctgt agtcggaatt ggtgatttta gcaataaagg gaaacatcaa tggttagaca
    53041 tgcttacaga ggatgacttt aaagaattga ccaaagttaa aactgtagga gaaatttgtt
    53101 gtcgattttt tgattcaaaa ggtaaagaag tctatgaaaa cttacaggaa agaacgatag
    53161 caatctcttt ggaagattta aaaaatattc ctcaaagttt agctgttgct tacggtgata
    53221 cgaaagtatc ttcgattctt tctgtcttgc gtgctaattt agtaaatcat ttgattacag
    53281 acaaaaatac aattttaaaa gttttggaag aagatgggga tttgactttt agagagattc
    53341 taggtgagtg aaaatgatag actgattcag tttatcgttt ttctttttag ttgattgcac
    53401 atttgtgctt atataaacaa aaatagttta tctgttgttt ttggattgac aactttatta
    53461 tgtagttgta ttctatagtt acaaaagaaa attttaaaat ttcaaatgaa aaaagctttt
    53521 tacatagtga aatgaggagg aatttatgga aatgattgtt ccagatcaaa ttatcatggg
    53581 tttaatttta tatgctggtg atgcgaaaca acatatttat aaagcgttag attacataaa
    53641 aaatggtaca tgtgaacggt gtgaagaaga aatacagtta gctgatgcag ccttattaga
    53701 agctcataat ctacaaacaa aatttttggc acaggaagcg tctggtacaa agacagaaat
    53761 tacagctctc tttgttcatt cacaagatca tctcatgacc agtatgacgg agattaattt
    53821 aatcaaagaa attattagtt tgagaaaaga acttcataaa aaataatact agagtattat
    53881 cattgttatt aacatagagg aggaaaacat aatggtgaag attggtttgt tttgtgcagc
    53941 aggtttttct actggtatgc ttgtaaataa tatgaaaatt gcagcgcaat ctagtggagt
    54001 tgaggcagaa atagaggcgt tttctcagtc taaattagcg gattatgcgc caaatataga
    54061 tgttgcacta ttgggtccac aagttgctta tacattagat aaatcaaaag aaatttgtga
    54121 taagtgtgat gttccgatag ctgttattcc gatgatggac tatggtatgt tagatgggaa
    54181 aaaagtatta gatttggccc tatctttgat tagtgggtaa gaaaaggaga tttattatgt
    54241 caaagatgga tgttcagaaa atcattgcac cgatgatgaa gtttgtgaat atgcgtggca
    54301 ttatagctct aaaagatggg atgttagcaa ttttgccatt gacagtagtt ggtagtttgt
    54361 tcttgattat gggacaattg ccgttcgaag gattaaataa gagcattgct agtgtttttg
    54421 gagctaattg gacagagccg tttatgcaag tatattcagg aacttttgct attatgggtc
    54481 taatttcttg tttttcaatt gcctattctt atgctaagaa tagcggagta gaggctttac
    54541 cagctggagt tctatctgta tctgcattct ttattttgct aagatcatct tatatcccta
    54601 aacaaggtga ggcgattggg gacgctatta gtaaagtttg gtttggaggc caaggaatta
    54661 tcggtgctat cattataggt ttggtagtag gaagtattta taccttcttt ataaagagaa
    54721 aaattgttat taagatgcca gaacaagttc cacaagctat tgccaaacag tttgaagcaa
    54781 tgattccagc atttgtaatt ttcttatctt ctatgattgt atatatttta gcgaagtcat
    54841 tgactaatgg cggaacattc atagaaatga tttattctgc tattcaagtt ccgttgcaag
    54901 gtttaactgg atctttgtat ggtgctattg gaattgcatt ctttatatca tttttgtggt
    54961 ggtttggtgt tcatgggcaa tcggtagtaa atggagtagt gacagctctg cttttatcta
    55021 atcttgatgc taataaagct atgttagcct ctgctaatct atcattagaa aatggtgcac
    55081 atattgttac tcaacaattt ttagattcat ttttaattct atcaggttca gggattacgt
    55141 ttggtcttgt agttgccatg ctttttgcag caaaatcaaa acaataccaa gccttaggaa
    55201 aagttgcagc ttttccagca atatttaacg taaatgagcc agttgtattt ggatttccga
    55261 ttgtcatgaa tccagttatg tttgtacctt tcattcttgt tcctgtactt gcagctgtga
    55321 tagtatatgg agctattgca acaggtttca tgcagccatt ctcaggggta acattgcctt
    55381 ggagtacacc agctatttta tcaggatttt tggtgggtgg atggcaagga gttattactc
    55441 agctggtgat attagcgatg tctacattgg tttattttcc attctttaaa gtacaggatc
    55501 gtttagctta ccaaaatgaa atcaaacaat cttagaggta tttgtgtgtt actgttaaac
    55561 tcacacattt gtgctaaaaa ttagagagtt aaaatttttc tagttaaaag cttgaaaatt
    55621 tctataaaaa tcggtattat attttcgaaa gaaataaaaa tattttcgaa agaaaggtgc
    55681 ttacgatggt aaatacagaa gtagcaagaa caacaatcaa gacagaatat tttggcagcc
    55741 ttactgaaag gatgaacaaa tatcgagaag atgttttaaa taaaaaacct tatattgatg
    55801 ctgagagagc agttctagca acacgcgcct atgaacgata caaggaacaa cctaatgtcc
    55861 taaaacgtgc atatatgctg aaagaaattt tggaaaatat gactatctat attgaagaag
    55921 aatctatgat tgcgggaaat caagcttctt ccaataaaga tgctcctatt tttccggaat
    55981 atacgctaga atttgttctc aatgagttgg atctttttga aaagcgtgat ggagatgttt
    56041 tctatattac agaagaaaca aaagaacaac ttagaagtat tgctccgttt tgggaaaata
    56101 ataatttacg tgctagagct ggtgccttat tacctgaaga agtgtctgtt tatatggaaa
    56161 caggattctt cggtatggaa ggtaagatga attctggaga tgctcactta gcagttaact
    56221 atcagaaact tttgcaattt ggtttaagag gttttgaaga gcgggctcgt aaagcgaaag
    56281 tagctctaga tttaacagat ccagcaagta ttgataaata tcatttttac gactctatat
    56341 ttattgtaat cgatgctatt aaagtatatg caaagcgctt tgttgctctt gctaaaagtt
    56401 tagccgaaaa tgcaaatcct aaacgtaaga aagaattact tgagattgca gatatttgct
    56461 ctagagtccc atatgaaccg gcaactactt ttgcagaagc tattcaatca gtttggttta
    56521 ttcaatgtat tttacaaatt gaatctaatg gccactctct ttcatatggc cgttttgatc
    56581 aatatatgta tccatatatg aaggctgatt tagaaagtgg taaagaaaca gaagatagca
    56641 ttgttgaacg tctgacaaat ctttggatta agacaattac aatcaataag gttcgcagtc
    56701 aatcacatac attttcttca gcaggaagtc ctttatatca aaatgttaca attggtggac
    56761 agactcgaga taagaaggat gctgttaacc tattatctta tttggtatta aaatcagttg
    56821 cacaaaccca tctaccgcaa cctaatctaa ctgtacgtta ccatgcaggt ttagatgctc
    56881 gtttcatgaa tgagtgtatt gaagtgatga aacttggttt tggtatgcct gcatttaata
    56941 atgatgagat tattattcct tcttttattg caaaaggagt attggaagat gatgcttatg
    57001 attacagtgc cattggatgt gttgaaacgg cagttccagg gaaatggggc tatcgttgca
    57061 caggtatgag ttatatgaac ttccctaagg ttctacttat cacgatgaat gatggaattg
    57121 atccggcttc gggtaaacgg tttgcaccaa gctttggtca ttttaaggat atgaagaact
    57181 tttctgaatt agaaaatgct tgggataaaa cactaagata tttgacacga atgagtgtta
    57241 ttgttgaaaa ttctattgat ttatcattgg aacgagaagt tcctgatatt ctatgttcag
    57301 cattgactga tgattgtatt ggtcgtggaa aacaccttaa agaaggtgga gcagtatatg
    57361 attatatatc aggattgcaa gttggaattg caaatttgtc ggattcatta gctgcaatta
    57421 aaaaattggt gtttgaggaa gaacgtataa gcccaagtca gctttggcat gcactggaaa
    57481 cagattatgc cggagaagaa ggtaaggtca ttcaagaaat gttgattcat gatgcaccta
    57541 agtatggtaa tgatgatgat tatgctgaca aattggttac tgctgcttat gacatttatg
    57601 ttgatgaaat tgctaaatat cctaatacac gttatggaag aggtccaatt ggaggaattc
    57661 gttattcagg aacatcttct atctcagcca acgtagggca gggacgtgga acattagcaa
    57721 ctccagatgg acgcaacgcg ggtacaccgt tagcagaggg ttgttcacca tcacataata
    57781 tggatcaaca cggccctaca tctgttttaa aatctgtttc aaaattacca acagatgaaa
    57841 tcgtaggtgg ggttctctta aatcagaaag taaatcctca aacgttagcc aaagaagaag
    57901 ataaattaaa actaattgct ttgttacgaa cattctttaa tcgtttacat gggtaccata
    57961 ttcaatacaa tgttgtttcc agagagacgc tgattgacgc tcagaaacat cctgaaaaac
    58021 acagagactt aattgttcgt gttgcaggat actctgcatt cttcaatgtt ctttctaagg
    58081 caacccaaga tgacattata ggacgtactg agcatacttt gtaaaataaa gaggttcttt
    58141 ttatggaatt tatgcttgac acattaaatt tagatgagat taaaaagtgg tctgaaattt
    58201 tgccgctagc tggggtaact tcaaatccca ctattgcaaa aagagagggt tctattaatt
    58261 tttttgaacg aatcaaagat gtaagagaat tgattggctc tacaccctct attcatgttc
    58321 aggtgatttc tcaagatttt gaaggcatct taaaggatgc tcataaaatt cgaagacaag
    58381 caggagatga tatatttatc aaagtacctg ttactccagc tggattacgt gcaataaagg
    58441 cgctaaaaaa agagggctac catatcactg caacagctat ttatacagtt attcagggat
    58501 tattagctat cgaagcagga gcggattacc tagctccata ttataataga atggaaaatc
    58561 tgaacattga ttcaaattct gtcattcgtc aattagctct tgctattgat agacagaact
    58621 ctcctagtaa gattttagct gcatccttta aaaatgtagc acaagtaaat aatgctttag
    58681 ctgcaggtgc gcatgctgtt acagcaggag cggatgtttt tgaatcagct ttcgccatgc
    58741 catctatcca aaaggcggtt gatgattttt ctgacgattg gtttgttatt caaaatagtc
    58801 gttccattta gatagagagg aaatacatat gagaattttt gctagtcctt ctagatatat
    58861 tcagggggaa aatgccttgt ttgaaaatgc caaatcaatt ttggatttgg gaaattgccc
    58921 tattctatta tgcgatcagt tggtttatga tattgttgga aaacgatttg aagattacct
    58981 acataggtat ggtttccata ttgttctggc gctatttaat ggtgaagctt ctgacaatga
    59041 aatcaatcga gttgttgcct tggctgagaa agaaaattgt gatagtatta tcggtcttgg
    59101 tgggggaaag acgattgata gcgcaaaagc tattgcagat ttgattgaaa agcctgttat
    59161 tattgctcca acaattgcat cgaccgacgc acctgtatct gctttatctg ttatttatac
    59221 agatgaaggt gcatttgatc attatctatt ttattctaaa aatccagatt tagttttggt
    59281 tgatacaaaa gttatttcac aagcccctaa gcgtttatta gcgtctggta ttgcagatgg
    59341 tttagcaact tgggttgagg cgcgtgcggt tatgcaggca aatggaaaaa ctatgttggg
    59401 acaacagcaa acattggctg gagttgcaat tgcgaagaaa tgtgaagaaa cgctgtttgc
    59461 agatggttta caggctatgg cagcttgtga agctaaagtg gtgacaccag cattagaaaa
    59521 tattgttgaa gctaatactt tattgagtgg tctaggtttt gaaagtggag gattagctgc
    59581 ggcgcatgca attcataatg gttttactgc attgacaggt gacattcatc atttaacaca
    59641 tggtgaaaaa gtagcttatg gaactttagt acaactatta ttggaaaata gacctaaaga
    59701 agaacttgat aagtatattg agttttacaa aaaaattggt atgccaacaa ctctaaaaga
    59761 aatgcatttg gatcaagttg gatatgatga tttaataaaa gttggtaaac aagcaactat
    59821 ggagggtgag acaattcatc agatgccgtt taagatttcg ccttcagatg ttgctcaagc
    59881 tattatcgct gtagatgcct atgtaaattc aaaataaaca ataaggacta ctgttttcca
    59941 aatggtagtc ttttattgat ccctgtattg aattctatag aagattgaaa taggatgaga
    60001 acaaatcgat tgggaaagta aaattaattt ctataaatgt tttagcaatt gtttcgtact
    60061 atttcagatt cagtctacta tatgttcttc ataaatcaaa aagcgacata ggttgtcggc
    60121 tatttattgt gaatacatta attagcattc cagttttatc ttcggtctaa aataagtatt
    60181 ttgtgctata cgagataagc ttcttgactt actccttgat ttactgcata acaatgggat
    60241 aaaaagtggg agatagagca attcatagtc atcaaaatta atgagataca gtatacagtt
    60301 tttcctttaa acacatttca aattccctca aaaatggtat aatagtaaca tcacaaaatt
    60361 ggagagagac catgagtttt tacaatcata aagaaattga gcctaagtgg cagggctact
    60421 gggcagaaca tcatacattt aagacaggaa cagatgcatc aaaacctaag ttttatgcgc
    60481 ttgatatgtt cccttatccg tctggagctg gtctgcacgt aggacaccca gaaggttata
    60541 ctgcaaccga tatcctcagt cgttacaaac gtgcgcaagg ctacaatgtc cttcacccaa
    60601 tgggttggga tgcttttggt ttgcctgcag agcaatacgc tatggatact ggtaatgacc
    60661 cagcagaatt tacagcggaa aacattgcca acttcaaacg tcaaattaat gcgcttggat
    60721 tttcttatga ctgggatcgt gaagtcaaca caacagatcc aaactactac aagtggactc
    60781 aatggatttt caccaagctt tacgaaaaag gcttggccta tgaagctgaa gtgccagtaa
    60841 actgggttga ggaattggga actgccattg ccaatgaaga agtgcttcct gacggaactt
    60901 ctgagcgtgg aggctatcca gttgtccgca aaccaatgcg ccaatggatg ctcaaaatca
    60961 cggcttacgc agagcgcttg ctcaatgact tagatgaact agattggtca gagtctatca
    61021 aggatatgca acgcaactgg attggtaaat caactggtgc caatgtaact ttcaaagtaa
    61081 aaggaacaga caaggaattt acagtcttta ctactcgtcc ggacacactt ttcggtgcga
    61141 ctttcattgt cttggctcct gaacatgaat tagtagacgc tatcacaagt tcagagcaag
    61201 cagaagctgt agcagactat aaacaccaag ccagccttaa gtctgacttg gctcgtacag
    61261 accttgctaa agaaaaaaca ggggtttgga ctggtgctta tgccatcaac cctgtcaatg
    61321 gtaaggaaat gccaatctgg attgcagact atgtccttgc tagttatgga acaggtgcgg
    61381 ttatggctgt gcctgcccac gaccaacgtg actgggaatt tgccaaacaa tttgaccttc
    61441 caatcgtcga agtacttgaa ggtggaaatg tcgaagaagc tgcctacaca gaggatggcc
    61501 tgcatgtcaa ttcagacttc ctagatggac tcaacaaaga agacgctatt gccaagattg
    61561 tggcttggtt ggaagaaaaa ggctgtggtc aggagaaggt tacctaccgt ctccgcgact
    61621 ggctctttag ccgtcaacgt tactggggtg agccaattcc aatcattcat tgggaagatg
    61681 gaacttcaac agctgttcct gaaactgaat tgccgcttgt cttgcctgta accaaggata
    61741 tccgtccttc aggtactggt gaaagtccac tagctaactt gacagattgg cttgaagtga
    61801 ctcgtgaaga tggtgtcaaa ggtcgtcgtg aaaccaacac tatgccacaa tgggctggtt
    61861 caagctggta ctacctccgc tatattgacc cgcacaatac tgagaaattg gctgatgagg
    61921 acctcctcaa acaatggttg ccagtagata tctacgtggg tggtgcggaa catgctgtac
    61981 ttcacttgct ttatgctcgt ttctggcata aattcctcta tgacctcggt gttgttccga
    62041 ctaaggaacc attccaaaaa ctctttaacc aagggatgat tttgggaaca agctaccgtg
    62101 accaccgtgg tgctcttgtg gcaaccgaca aggttgaaaa acgtgatggt tccttcttcc
    62161 atgtagaaac aggggaagag ttggagcaag cgccagccaa gatgtctaaa tcgctcaaga
    62221 acgttgttaa cccagacgat gtggtggaac aatacggtgc cgataccctt cgtgtttatg
    62281 aaatgtttat gggaccactc gatgcttcga ttgcttggtc agaagaaggt ttggaaggaa
    62341 gccgtaagtt ccttgaccga gtttaccgtt tgattacaag taaagaaatc cttgcggaaa
    62401 acaatggtgc tcttgacaag gcttacaacg aaacagtcaa agctgttact gagcaaattg
    62461 agtctctcaa attcaacaca gctattgccc aacttatggt ctttgtcaat gctgctaaca
    62521 aggaagataa gctttatgtt gactatgcca aaggctttat tcaattgatt gcaccatttg
    62581 cacctcactt ggcagaagaa ctctggcaaa cagtcgcaga aacaggtgag tcaatctctt
    62641 atgtagcttg gccaacttgg gacgaaagta aactggttga agacgaaatc gaaatcgttg
    62701 tccaaatcaa aggtaaagtc cgtgccaaac tcatggtcgc taaagaccta tcacgtgaag
    62761 aattgcaaga aatttctcta gcggatgaaa aagtcaaagc agaaattgac ggtaaggaaa
    62821 tcgtgaaagt gattgcagta ccgaataaat tggttaatat tgttgtgaaa taaccaattt
    62881 atttgctggt ttcaaatctc ggacagtttc ctaaactgtt tgagccagtt aaactcgtta
    62941 atattgtttt gaaataagat aggagtcctt cagagtagaa tctggaggat tttttgaatc
    63001 ttcttatgaa agtatgatat actatgggca actataaagt ttgaaaagtg aaataaggag
    63061 aataagatgc cagtaaatga atatggtcaa atgattgggg agtcaatgga agcttatact
    63121 ccaggtgaat tgccttcttt tgatttctta gaagggcgtt atgctaggat agaggctctt
    63181 tcagtggaaa agcatgcgga ggatttatta gctgtttatg gccctgatac gcctcgggag
    63241 atgtggacct acctctttca ggagtcagta gcagacatgg aggaactggt cagcctttta
    63301 aatcagatgt tggctcgtaa ggaccgtttt tattatgcaa tcatagacaa ggcaactggt
    63361 aaggctttgg gaactttttc cctcatgcga attgatcaga ataaccgagt aatagaagtg
    63421 ggagctgtca ctttttctcc agagctcagg gggacacgga taggaacaga agcccagtat
    63481 ctcttggctt gctatgtctt tgaggagctt aactatcgtc gctatgagtg gaaatgcgat
    63541 gctcttaacc tgccatccag acgagcagcg gaacgtttgg gatttattta tgaaggaacc
    63601 ttccgtcagg cagtggttta taaggggcgt acaagagata cggattggtt gtctatgatt
    63661 gataaggact ggcctcaagt caaagctcga ttggaaatat ggttgcgtcc tgaaaacttt
    63721 gataaaaatg gacgacagca caagagcttg agagaacttt aagaggtgtt gagatgatta
    63781 ctattaaaaa gcaagaaatt gtcaagctag aggatgtttt gcatctctat caggctgtcg
    63841 gttggacaaa ctatacccat caaacagaga tgctggagca ggccttatct cattcattag
    63901 taatttatct ggcacttgat ggtgatgctg tggtgggctt gattcgtttg gttggagatg
    63961 gtttttcatc agtttttgta caggatttga ttgttttgcc tagctatcag cgtcaaggga
    64021 ttggtagctc cttgatgaaa gaggctttag gaaattttaa agaggcctat caagtccagc
    64081 tggcgacaga agagacagaa aaaaacgtgg gattttatcg ttctatgggc tttgaaatct
    64141 tatccaccta tgactgtaca ggaatgattt ggataaacag agaaaaataa aaaaacttgt
    64201 ttgttcttaa gcaaagttta aggatggtct agtatcatat agtcattaaa taaagacctc
    64261 ctaactttat ttaataaaat cctaaacttt tttcatcaca atctcctaat gaagccaccc
    64321 aatcaggtgg cttttttgcg gtacgacggg catgtcgtat atctgaggtg taagtcctca
    64381 gcctgactat cgtgaggtag cagggagagg aagggatagc gaaatcgtgg ctctacgaac
    64441 aggaacgtga tagtaaggcg tatatagcgg ataaggaggc ttcaaactct aaagtccaaa
    64501 aaggtagtcg taacctatat gtgtaaatca cgagagtaat tgaattcgga ctaaggtttg
    64561 tgtgaaaaag ataaatcttt ctagagtcta aagactctgc gtcagatttc ctattttcac
    64621 tgtaaccttt taacgtcctc atatcttgta taaacgagga aagatgtacg acttatcccg
    64681 tgaggtttca tgagcgctga aagcgtagta acaacgaatc atgagaagtc agccgagccc
    64741 atagtagtga ggaaacttcc gtaatggaag tggagcgaag gggtgaatac tcaaacagtc
    64801 tggggagaga ctgtttgagg tctgtcgcta gaaagagaaa acgacagatc gaagtaatcc
    64861 tacttcactt gtgtctgtaa aatgagtggt ctgatagaac tggactttga cacgtaatga
    64921 ctagatgaag gttcaccaat ataagatgtc cctcaggcac caaaataaga aaggaatacg
    64981 cacatgtcaa aactgctaga taagatatta tcacgcgaaa atatgctgga agcctacaat
    65041 caagtaaaat ccaataaagg ctcagctggg attgatggaa tgactatcga agagatggat
    65101 aattatctca gacaaaactg gcgcttgact aaggaactga taaaacagag aaaatataag
    65161 cctcaaccag ttcttagagt tgagatacct aaaccagacg gaggcatccg tcaactagga
    65221 attccaacag ttatggatag aatgattcaa caggccattg tccaagtcat gagccccatt
    65281 tgtgaacccc atttctcaga tacgagttat ggtttcagac caaataggtc atgtgaaaaa
    65341 gccatcatga agctcttaga atacttaaat gacggctatg agtggatagt ggattagacc
    65401 tagagaaatt tttcgataca gttcctcaag atagattgat gtccttagta cataacatta
    65461 tcgaagacgg agatacggaa tccttgattc gtaagtatct tcattcaggt gttatcatta
    65521 atggtcaacg ttataaaaca ctagttggta caccacaggg aggaaattta tctcctctct
    65581 tatccaatat catgcttaat gaattggaca aggaattaga aaagagggga cttcgatttg
    65641 tgcgctacgc agatgattgt gtgattacgg tcggaagcga ggcagcctct aagcgtgtga
    65701 tgtattcagt cagtcgtttt attgagaaac ggctaggttt gaaagtaaac atgaccaaga
    65761 gagttgaaat atctaggttt tgggttctgg aaattatcag atggttggaa aagccgtcca
    65821 catcaagata gtgttcggag atttaagttt aaattgaaga aactaacaca gaggaaatgg
    65881 agtatagacc taacaagacg tattgagcaa ctgaatttgt ctattcgagg atggataaac
    65941 tattgctcat tgggaaatat gaaaagtata gtcgccagca tagatgagcg cttgcgtact
    66001 cgcctacgag tgattatctg gaagcaatgg aagaagaaat cgagacgatt atggggattg
    66061 cttaagttag gagttcctaa atggatagca gataaggtat ctggctgggg cgaccattat
    66121 caattagtag ctcagaagtc ggtacttaaa cgtgctatat caaaaccagt cctggaaaaa
    66181 cgtggactgg tttcgtgttt ggattattac cttgaacgac atgcgttaaa agttagttga
    66241 accgccgtat gccaaacggc acgtacggtg gtgtgagagg ggctagagat tatcccctac
    66301 tcgattaact cccctgaaat ttattttaat tatgcaaatt tcacgtattt ttgatgctga
    66361 gacgacgatc ctgggaactt ttcagatatt tttttgacta tctaaatcta tcattagaaa
    66421 agcttagagc gccaaaggat ttgagcgttt ttctgatttt taagactttt tccagtctct
    66481 ttttcgattg aagatgtaat tattctacta actaactaac ttcttagtac tagccaacaa
    66541 cgataatcat aattcctcct aaaattagga ataataaagg caatagtttt tgttttttca
    66601 tgtaaaaaac ctcacttttg ttttctgcta ttttatgcta aaatattaaa aatcaaattt
    66661 aattccaaag tttgtaacta aagggggagc gctacatgtc taattcattt gtcaagttgt
    66721 tagtctctca attatttgca aatttagcag atattttctt tagagtaaca atcattgcta
    66781 acatatacat tatttcaaaa tcagtaattg ccacatcact agttcctatc ttaataggaa
    66841 tatcctcttt tgttgcgagt cttttagttc cgttggttac taaaaggtta gcgctaaata
    66901 gggttttatc tttatctcaa tttggaaaga ctatattatt ggcgatactg gtaggaatgt
    66961 ttaccgtaat gcaatccgta gcgcctttgg tgacctatct atttgttgtt gcaatttcca
    67021 tactagatgg ttttgcagca cccgtttcct atgctattgt gccacgctat gcgaccgatt
    67081 tgggtaaggc taattcagcc ttatcaatga ctggtgaagc tgttcaattg ataggttggg
    67141 gattaggtgg actcttgttt gcaacaattg gtctgttacc taccacgtgt atcaatttag
    67201 tcttgtatat catttctagc tttctgatgt tatttcttcc taacgctgaa gtggaggtgt
    67261 tagagtcaga aactaatctt gaaattttgc tcaaaggttg gaagttagtt gctagaaatc
    67321 ctagattaag actttttgta tcagcaaatt tattggaaat tttttcaaat acgatttggg
    67381 tttcttccat tatacttgtt tttgtaacgg agttattaaa taaaacggaa agttactggg
    67441 gatattctaa tacagcatac tctattggta ttataattag tggcttaatt gcttttaggc
    67501 tatctgaaaa gttccttgct gctaaatggg aaagtattct ttttcctctt gtagctatgg
    67561 aaatagtgac actgactatt ctatattttc caaacgcaca gatgttttta ttattttcag
    67621 ctttagttgg tatgttatcg caactaaaag aagttcctga aagtgtattt cttcaggaaa
    67681 cagtagagga aaatcattta gttaatgtct attccgttct tgaagtgatt tctacattag
    67741 cattttcagt atttgttttg ctaatgagct atattactga gagttttggt attagcatca
    67801 gtttttggct atcagccatt tgtctgatga tagaagctat tttaatttat atcagacgag
    67861 attattttaa atgagagagt ggttaaaaag tctttaaaaa tcagaaaagc gcatagtatc
    67921 aggtgttgaa aaaacttgat attgtgtgtt ttattatggg aagatttact tcattgtctc
    67981 ctgaaatgga gttttgaagc aggatcactc tatttgaaaa tatacataga aaaactgtat
    68041 aatccttaaa ataagctgaa atctattgga aacatacggt aaacaattga aaagatacta
    68101 tataaagtgg tacaatggta gaaaaataag ctagtaagaa tcactcttat tttagcgaaa
    68161 aattacagaa atgaatggtt tttcttgatg aaacagagaa aagaattgta cctctttctt
    68221 ggtcggacag ccttgtattt tcttatcttt ctagggctgc tttacttctt tagctatctt
    68281 ggtcagggtc aaggaagctt tatctataat gaattttaac ttgaaggaga atccctattg
    68341 tgtcaaataa accaatagca gatatgattg aaaccattga gcattttgct cagacacagc
    68401 ctagctatcc tgtctataat gttttggggc aggaacacac ttatggcgat ttaaaggctg
    68461 attcggatag tttggctgca gtcattgacc aactaggctt gcctgagaag tctcctgtgg
    68521 ttgtttttgg tggccaagaa tatgaaatgt tggcaacctt tgtagcgctg actaagtcag
    68581 gtcatgccta cattccaatt gatagccatt cggccttgga gcgagtttca gctattttag
    68641 aagtagcaga gccaagcttg attattgcca tttcagcctt tcccttggag caggtttcta
    68701 caccaatgat aaatctagct caggttcaag aagcctttgc ccaagggaat aactatgaaa
    68761 tcacgcatcc agtcaaggga gatgataatt actacattat ctttacttct ggtacgactg
    68821 gtaagcctaa gggagtgcag atttcacatg ataatctcct cagctttaca aactggatga
    68881 ttacggataa ggaatttgcg acaccgagtc gtccgcaaat gctggcacag ccaccttatt
    68941 cttttgactt gtctgtcatg tattgggcac cgaccttggc acttggtggt acgcttttca
    69001 ctcttccttc agtcatcact caggacttta agcaactctt tgcggctatc ttttcattgc
    69061 caatcgctat ctggacatca acaccatcct ttgcagatat ggccatgttg tctgaatact
    69121 tcaacagtga gaaaatgcct ggaatcacgc atttctactt tgatggtgaa gaattgacgg
    69181 tcaaaacagc tcaaaaactg cgcgagcgtt tcccaaatgc ccgtatcatc aatgcttacg
    69241 gcccaacaga agcgacagta gctctgtcag cagttgccgt gacagacgag atgttagcga
    69301 ctctcaaacg cctaccaatc ggctatacca aggctgattc tccaaccttt atcattgacg
    69361 aggaaggaaa taaactgcca aatggtgagc agggagaaat cattgtttct gggccagctg
    69421 tttcaaaagg ttatatgaac aatcctgaaa aaacagcaga agccttcttt gagtttgaag
    69481 atctgccagc ctatcacaca ggcgatgtgg gaaccatgac agatgagggc ttgcttctct
    69541 acggcggacg catggacttc cagattaagt ttaacggtta ccgcattgag ttagaagatg
    69601 tctctcaaaa cctcaacaag tctcgcttta tcgaatctgc tgtcgcagta ccgcgctata
    69661 acaaggacca caaggtacaa aatctattgg cttatgtcat cttaaaagac ggtgttcgtg
    69721 agcagtttga gcgagatatc gatattacca aggccatcaa ggaagacctg acagacatca
    69781 tgatgtccta tatgatgcca tctaaattcc tttaccgaga cagtttgcca ctaactccaa
    69841 atggaaagat tgacatcaaa ggattgatta acgaggtgaa taagagatga tggagttttt
    69901 tcaacagctt cctcatttag agccatatgg caatcctcag tattttgttt atgtgattgc
    69961 tgcaaccttg cccatcttta taggtctctt tttcaagaaa cgctttgcct ggtatgaagt
    70021 gttggtaagt ctcttcttta ttgtcaccat gttggtgggt ggaaagacca atcaactagc
    70081 tgccttgggt atttaccttt gctgggaaat attgctcctg cttttctaca agcattatcg
    70141 aaaaagcaag gatggcaagt gggtcttcta cttagttagt tttctgtccc tacttccgat
    70201 tatctttgtc aaggtgcaac cagctatcaa tggaacgcag tctttgcttg ggttcttggg
    70261 aatttcttac ctgacctttc gttcggttgg aattgtcatc gatctgagag atggagtgat
    70321 taaggatttt accctctggg aattcctccg tttccttctc ttcatgccaa ctttctcgag
    70381 tggtccaatc gatcgcttta agcgatttaa tgaaaattat caggctattc ctgagcgaga
    70441 tgagttgatg gatatgctgg atgaatctgt ccgctatatc atgtggggct ttttgtataa
    70501 gtttatccta gctcatgttt taggagagac cttactacct cctctgaaga atttagcctt
    70561 gcagtcaggt ggcttcttta atctctatgc cttggcagtt atgtatactt ttggtctgga
    70621 actcttcttt gactttgcag gttattctat gtttgctttg gccatctcaa acttgatggg
    70681 aatccgtagc cctatcaact ttaacaagcc ctttttatca agggatttaa aggagttttg
    70741 gaatcgctgg catatgagtc tgtccttctg gttccgtgac tttgtcttta tgcgaatggt
    70801 gatggtgtta accagaaaga aagtctttaa aaatcgtaat gtaacctcaa gcatggccta
    70861 cattgtaaat atgctgatta tgggattttg gcatggtgtg acctggtact atatcgccta
    70921 tggactcttt catggactag gcttggtcat caatgatgcc tgggttcgca agaaaaaaac
    70981 gctcaataag gaacggaaaa aagcagggaa ggctgcccta cctgagaatc gctggattca
    71041 gttgcttggc atggttgtca ctttccatgt tgtcatgttg tcattcttaa tcttttctgg
    71101 attcttgaat aatctatggt ttaaaaaata aaaggaaata aaatatggat atcaaatcag
    71161 aagttattga aattattgat gagttgttta tggaagatgt ttctgacatg atggatgaag
    71221 atctttttga tgcaggtgtc ttggatagta tgggaacggt tgagttgatt gtggagattg
    71281 agaaccgttt tgacattcgt gtccctgtaa cagagtttgg tcgtgacgac tggaatacag
    71341 ctaataaaat catagctggt attgtggagc tacaaaatgc ttaaacgctt atggatgatc
    71401 ttcggaccgg tcttgatcgc tggtttgttg gtttttctgc tcattttctt ttatcctact
    71461 gagatgcatc ataatctagg agctgaaaag cgttcagcag tggctactac tatcgatagt
    71521 tttaaggagc gaagtcaaaa agtcagagca ctatctgatc caaatgtgcg ttttgttccc
    71581 ttctttggct ctagtgaatg gcttcgtttt gacggtgctc atcctgcggt attagctgag
    71641 aaatacaatc gttcctaccg tccttatctt ttaggacagg ggggagctgc atcgcttaac
    71701 caatattttg gaatgcaaca gatgttacca cagctggaga ataaacaagt tgtgtatgtt
    71761 atctcacctc agtggttcag taaaaatggc tatgatccag cagccttcca gcagtatttt
    71821 aatggagacc agttgactag ttttctgaaa catcaatctg gggatcaggc tagtcaatat
    71881 gcagcgactc gcttactgca acagttccca aacgtagcta tgaaggacct ggttcagaag
    71941 ttggcaagta aagaagaatt gtcgacagca gacaatgaaa tgattgaatt attggctcgt
    72001 tttaatgaac gccaagcttc cttttttggt cagttttcgg ttagaggcta tgttaactac
    72061 gataagcatg tagctaagta tttaaaaatc ttgccagacc agttttctta tcaggcaata
    72121 gaagatgttg tcaaagcaga tgctgaaaaa aatacttcca ataatgagat gggaatggaa
    72181 aattatttct ataatgagca gatcaagaag gatttgaaga aattaaagga ttctcagaaa
    72241 agctttacct atctcaagtc gccagagtat aatgacttgc agttggtttt aacacagttt
    72301 tctaaatcta aggtaaaccc gatttttatc attccacctg ttaataaaaa atggatgaac
    72361 tatgctggtc tacgagagga tatgtaccaa caaacggtgc agaagattcg ctaccagtta
    72421 gaaagtcaag gttttaccaa tatagcagat ttttctaagg acggcgggga gcctttcttt
    72481 atgaaggaca ccattcacct tggttggttg ggttggttgg cttttgacaa ggcagttgat
    72541 cctttcctat ccaatcccac accagctccg acttaccatc tgaatgagcg ctttttcagc
    72601 aaagattggg cgacttatga tggagatgtc aaagaatttc aatagataat ataatataga
    72661 agagttcaag aatgaaacta gttttcacgt tttttcttga ctcttttttt gcttgtgata
    72721 taattaactg gtaaacaaaa tgtcaaagaa tagtattttt ctcttaaaaa agagaagtct
    72781 aaaaggaaag gagtcagata tgagacagct agcaaaggat atcaatgctt ttttgaatga
    72841 ggtgattttg caggcggaaa atcagcatga aatcctaata ggtcattgca ctagcgaggt
    72901 ggccctgacc aatactcagg agcatatcct tatgctcttg tcagaggaat ctttaacaaa
    72961 ttcagaattg gcccgtcgtc tcaatgtcag tcaggcggca gttaccaagg ccattaagtc
    73021 tttggtcaag gaagggatgt tggaaacatc taaagattct aaagatgcgc gtgtgatttt
    73081 ttatcagttg actgacttgg ctcgtccaat cgctgaggag caccaccatc accatgagca
    73141 tacactttta acctatgaac aagtggcgac tcagtttact ccaaatgaac aaaaagtgat
    73201 tcagcggttt ttgactgctt tagtaggaga aatcaaataa tgagatatat tacggtagag
    73261 gatttgtcct tctattatga taaggagcct gttcttgaac atatcaatta tagtgttgat
    73321 agtggggaat ttgttacctt gactggggaa aatggagcgg ctaagacgac gctcatcaag
    73381 gccagtcttg gaattctgca accacgcatt ggaaaggtgg ctatttcaaa gacaaatacg
    73441 caaggtaaca aattgagaat agcctatctt cctcaacaaa ttgccagttt taatgctggt
    73501 tttccaagta cggtctatga atttgtcaag tcgggtcgct atccgagaaa aggctggttc
    73561 cgtcgtttga atgctcatga tgaggagcat atcaaggcca gtctggactc agttggcatg
    73621 tgggaacatc gagacaaacg cttggggtct ctatctgggg gacaaaagca gcgagcggta
    73681 attgcgcgta tgtttgcttc tgaccctgat gtgtttatcc tagacgagcc gacaacgggg
    73741 atggatgcag gaagtaaaaa tgaattttac gaactcatgc accacagcgc ccatcatcat
    73801 ggcaaggctg ttttgatgat tacccatgac cctgaagaag ttaaggatta tgcggatcgc
    73861 aatattcatc tagtccgtaa ccaagactcg ccatggcgtt gtttcaacgt tcatgagaat
    73921 ggccaggagg tgagccatgc ttagtttatt atcttacgac tttatacaac gcgccttttt
    73981 ggcggttatt gctatgagtc ttttctcacc ggtattggga accttcctca tcttgcgtcg
    74041 tcagagtttg atgagtgata cccttagcca cgtctcactt tcaggtatag cctttggtct
    74101 ggttttgggg atttctccaa ctgtttctac tattgccatt gtcttgattg cggcggtctt
    74161 tctggagtat ctccgtacgg tttacaagag ctttatggaa atcgggacag ctatcctcat
    74221 gtcaacaggt ctggctgttt ctctgattgt catgagcaag ggtaaaagct cgagttcaat
    74281 gagtttggac caatatctct ttggttcgat cgtgactatc agtgaagaac aggtcatttc
    74341 cctctttgtc attgcggcgg ttgttttgat tttgaccttt ctctttcttc gtcctatgta
    74401 tatcttaact tttgacgaag atacggcctt tgtggatggc ttgccagttc gtaccatgtc
    74461 cattcttttt aacatggtga caggggtggc tattgccctt atgattcctg cagcaggagc
    74521 tcttctggta tcgaccatta tggtcttgcc agctagtatt gccctgcgtc tggggaaaaa
    74581 ctttaaatcg gttatgctgc ttgccagtgc gattggcttt ttgggaatgg tagcaggact
    74641 ttacatttcc tactatgcag aaacacctgc aagtgcaagt attaccatta tttttgtaac
    74701 tgtctttata ctaatcagtt tagtaagacg ttttatcaaa taggagacga acgtgaaaaa
    74761 aattagctta ttactagcca gtctatgtgc cttgttttta gtggcttgtt ccaatcaaaa
    74821 acaggcagat ggtaaactca atatcgtgac aaccttttac cctgtctatg aatttaccaa
    74881 gcaagtcgca ggagatacgg ctaatgtaga actcctaatc ggtgctggga cagaacctca
    74941 tgaatacgaa ccatctgcca aggcagttgc caaaatccaa gatgcagata ccttcgttta
    75001 tgaaaatgaa aacatggaaa catgggtacc taaattgcta gataccttgg ataagaaaaa
    75061 agtgaaaacc atcaaggcga caggcgatat gttgctcttg ccaggtggca aggaagaaga
    75121 gggagaccat gaccatggag aagaaggtca tcaccatgag tttgaccccc atgtttggtt
    75181 atcaccagtt cgtgccatta aactagtaga gcacatccgc gacagcttgt cagcagatta
    75241 tcctgataaa aaagagacct ttgagaagaa tgcagctgcc tatatcgaaa aattgcaagc
    75301 cttggataag gcttacgcag aaggcttgtc tcaagcaaaa caaaagagct ttgtgactca
    75361 acacgcagcc tttaactatc ttgccttgga ctatggactc aaacaagtcg caatctcagg
    75421 actttctcca gatgcagagc catcagctgc tcgcttggca gaattgacag agtatgtcaa
    75481 gaaaaataaa atcgcctata tctattttga agaaaatgcc tcacaagccc ttgctaacac
    75541 actttcaaaa gaagcaggtg tcaaaactga tgtcctcaat cctttagaaa gtctgacaga
    75601 agaggacacc aaggctggag aaaactacat ttccgtgatg gagaaaaacc tcaaggcttt
    75661 gaaacaaaca acagaccaag aaggcccagc aatcgaacct gaaaaggcag aggataccaa
    75721 gacagtccaa aatggttact tcgaggatgc agctgtcaag gaccgcacct tgagtgacta
    75781 tgcaggtaac tggcaatcag tttatccttt ccttgaagac ggcacgtttg accaagtctt
    75841 tgactacaag gctaagttga ctggtaagat gacccaggct gagtacaagg cttactatac
    75901 aaaaggctat cagacagatg tgactaagat taacattact gataatacta tggaatttgt
    75961 tcaaggtgga caaagcaaga aatacactta caagtatgtc ggtaagaaaa ttttgactta
    76021 caagaaaggc aatcgtggcg tgcgtttcct ctttgaagcc acagatgctg acgctggaca
    76081 attcaagtat gttcagttta gtgaccacaa tgttgcccca gttaaggcag aacatttcca
    76141 tatcttcttt ggaggcacaa gccaagaagc cctctttgaa gaaatggaca actggccaac
    76201 ctactaccca gataacctat ctggccaaga aatcgcccaa gaaatgttgg cgcattgatg
    76261 agaaatcgac tagttcataa gagctagtcg gttttttgat actagatggc taacttcatt
    76321 atagaccttt cagaacttt
//
LOCUS       DAVHEX010000002        74051 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVHEX010000002 DAVHEX010000000
VERSION     DAVHEX010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 74051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74051
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.002"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            253..1383
                     /gene="ugpC"
                     /locus_tag="VVL45_000088"
     CDS             253..1383
                     /gene="ugpC"
                     /locus_tag="VVL45_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEU4186350.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFITVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            complement(1530..1676)
                     /locus_tag="VVL45_000089"
     CDS             complement(1530..1676)
                     /locus_tag="VVL45_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4186351.1"
                     /translation="MEIKEQTRKLAVSCSKYIFEVADKTDEISNHTYGKATLTWFEEI
                     FEEY"
     gene            1884..2642
                     /locus_tag="VVL45_000090"
     CDS             1884..2642
                     /locus_tag="VVL45_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU4186352.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKALTGEMTLMKLSGMIYDEGLLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            2729..3241
                     /locus_tag="VVL45_000091"
     CDS             2729..3241
                     /locus_tag="VVL45_000091"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEU4186353.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            3423..4367
                     /locus_tag="VVL45_000092"
     CDS             3423..4367
                     /locus_tag="VVL45_000092"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEU4186354.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYGYL"
     gene            4376..5053
                     /locus_tag="VVL45_000093"
     CDS             4376..5053
                     /locus_tag="VVL45_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEU4186355.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVHSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFIN
                     AMDLWKD"
     gene            5151..5909
                     /locus_tag="VVL45_000094"
     CDS             5151..5909
                     /locus_tag="VVL45_000094"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEU4186356.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            5947..7452
                     /gene="lytC"
                     /locus_tag="VVL45_000095"
     CDS             5947..7452
                     /gene="lytC"
                     /locus_tag="VVL45_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HEU4186357.1"
                     /translation="MSVTFFIGEERLKTKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSTEVQTNAAAHWYGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAENDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTDTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPYYSGEKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            7948..8466
                     /locus_tag="VVL45_000096"
     CDS             7948..8466
                     /locus_tag="VVL45_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEU4186358.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
     gene            8596..9102
                     /locus_tag="VVL45_000097"
     CDS             8596..9102
                     /locus_tag="VVL45_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEU4186359.1"
                     /translation="MTKKIVAIWAQDEEGVIGKENRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQAQEKNLYIIGG
                     KQIFQAFEPYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            9102..9272
                     /locus_tag="VVL45_000098"
     CDS             9102..9272
                     /locus_tag="VVL45_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4186360.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            9304..10536
                     /gene="clpX"
                     /locus_tag="VVL45_000099"
     CDS             9304..10536
                     /gene="clpX"
                     /locus_tag="VVL45_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEU4186361.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            10545..11132
                     /locus_tag="VVL45_000100"
     CDS             10545..11132
                     /locus_tag="VVL45_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEU4186362.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            11143..11523
                     /locus_tag="VVL45_000101"
     CDS             11143..11523
                     /locus_tag="VVL45_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU4186363.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            11575..12465
                     /gene="rapZ"
                     /locus_tag="VVL45_000102"
     CDS             11575..12465
                     /gene="rapZ"
                     /locus_tag="VVL45_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEU4186364.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKCLAQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            12462..13439
                     /locus_tag="VVL45_000103"
     CDS             12462..13439
                     /locus_tag="VVL45_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEU4186365.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            13436..14347
                     /gene="whiA"
                     /locus_tag="VVL45_000104"
     CDS             13436..14347
                     /gene="whiA"
                     /locus_tag="VVL45_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEU4186366.1"
                     /translation="MSFTVAVKEEILGQHHLSRHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHYQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(14381..15346)
                     /locus_tag="VVL45_000105"
     CDS             complement(14381..15346)
                     /locus_tag="VVL45_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU4186367.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEFAITTSK
                     GSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSAV
                     DWALAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNGKTLDKLE
                     ITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYAI
                     GDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            15482..16339
                     /locus_tag="VVL45_000106"
     CDS             15482..16339
                     /locus_tag="VVL45_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEU4186368.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            16326..17105
                     /locus_tag="VVL45_000107"
     CDS             16326..17105
                     /locus_tag="VVL45_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEU4186369.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            17129..18481
                     /locus_tag="VVL45_000108"
     CDS             17129..18481
                     /locus_tag="VVL45_000108"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEU4186370.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            18555..18929
                     /locus_tag="VVL45_000109"
     CDS             18555..18929
                     /locus_tag="VVL45_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEU4186371.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            18931..19779
                     /locus_tag="VVL45_000110"
     CDS             18931..19779
                     /locus_tag="VVL45_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU4186372.1"
                     /translation="MKLAVFTDSSAYLSEETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNDPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            20045..20812
                     /locus_tag="VVL45_000111"
     CDS             20045..20812
                     /locus_tag="VVL45_000111"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEU4186373.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLPDSDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            20824..22008
                     /locus_tag="VVL45_000112"
     CDS             20824..22008
                     /locus_tag="VVL45_000112"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEU4186374.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLENISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQNKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQARGKEVNPQAITEKYQ
                     SLTIHDKKEIQINGDILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            22005..23876
                     /locus_tag="VVL45_000113"
     CDS             22005..23876
                     /locus_tag="VVL45_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU4186375.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELERAGLTEYQGNYQDYVRLKAEQDERDATLLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQDVSFAYENKPILQD
                     FNLLVQVKDRIGIVGDNGVGKSTLLNLIAGSLEPIAGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKAEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(24019..25203)
                     /gene="mntE"
                     /locus_tag="VVL45_000114"
     CDS             complement(24019..25203)
                     /gene="mntE"
                     /locus_tag="VVL45_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HEU4186376.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDILRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            25507..28203
                     /locus_tag="VVL45_000115"
     CDS             25507..28203
                     /locus_tag="VVL45_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEU4186377.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LLEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALITFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            28216..29007
                     /gene="yghU"
                     /locus_tag="VVL45_000116"
     CDS             28216..29007
                     /gene="yghU"
                     /locus_tag="VVL45_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEU4186378.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTENVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            29123..29533
                     /locus_tag="VVL45_000117"
     CDS             29123..29533
                     /locus_tag="VVL45_000117"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU4186379.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETTRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            29545..31140
                     /locus_tag="VVL45_000118"
     CDS             29545..31140
                     /locus_tag="VVL45_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4186380.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPRSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTN
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESINGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKLR"
     gene            31207..31371
                     /locus_tag="VVL45_000119"
     CDS             31207..31371
                     /locus_tag="VVL45_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HEU4186381.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
     gene            31450..32178
                     /gene="yaaA"
                     /locus_tag="VVL45_000120"
     CDS             31450..32178
                     /gene="yaaA"
                     /locus_tag="VVL45_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEU4186382.1"
                     /translation="MKILIPTAKEMNTDFPSIEAIPLKPESQAVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPVLSPMAPHRLDFLMKLKVAGKTLKSHWKAAYDETLKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(32455..33003)
                     /locus_tag="VVL45_000121"
     CDS             complement(32455..33003)
                     /locus_tag="VVL45_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU4186383.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIK"
     gene            33324..33812
                     /locus_tag="VVL45_000122"
     CDS             33324..33812
                     /locus_tag="VVL45_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEU4186384.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISSSFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKIIFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            33809..34996
                     /locus_tag="VVL45_000123"
     CDS             33809..34996
                     /locus_tag="VVL45_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU4186385.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAAVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTAILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            34989..35213
                     /locus_tag="VVL45_000124"
     CDS             34989..35213
                     /locus_tag="VVL45_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEU4186386.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEV"
     gene            35370..36716
                     /gene="asnS"
                     /locus_tag="VVL45_000125"
     CDS             35370..36716
                     /gene="asnS"
                     /locus_tag="VVL45_000125"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEU4186387.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPVAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            36869..37159
                     /locus_tag="VVL45_000126"
     CDS             36869..37159
                     /locus_tag="VVL45_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEU4186388.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            37171..37641
                     /gene="ssbA"
                     /locus_tag="VVL45_000127"
     CDS             37171..37641
                     /gene="ssbA"
                     /locus_tag="VVL45_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HEU4186389.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVLDFSRNENPFGATNPLDISDDDLPF"
     gene            37673..37912
                     /locus_tag="VVL45_000128"
     CDS             37673..37912
                     /locus_tag="VVL45_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEU4186390.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            38207..39607
                     /locus_tag="VVL45_000129"
     CDS             38207..39607
                     /locus_tag="VVL45_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEU4186391.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYTDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFDALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGNPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            39609..39968
                     /locus_tag="VVL45_000130"
     CDS             39609..39968
                     /locus_tag="VVL45_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEU4186392.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            40022..40771
                     /locus_tag="VVL45_000131"
     CDS             40022..40771
                     /locus_tag="VVL45_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEU4186393.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPYSNLNESEHYLLARHEITTNLEEICHSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            40761..41060
                     /locus_tag="VVL45_000132"
     CDS             40761..41060
                     /locus_tag="VVL45_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEU4186394.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDMRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            41131..42066
                     /locus_tag="VVL45_000133"
     CDS             41131..42066
                     /locus_tag="VVL45_000133"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEU4186395.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            42153..42665
                     /locus_tag="VVL45_000134"
     CDS             42153..42665
                     /locus_tag="VVL45_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEU4186396.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACRHLGAPYNASFYPDAAGFYCSQ
                     YIAEILPIFETIPMKFGDEEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            42690..43298
                     /locus_tag="VVL45_000135"
     CDS             42690..43298
                     /locus_tag="VVL45_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEU4186397.1"
                     /translation="MFGELIRYLDQYEDVILREIKAQFPDVAVDKLMEEYIKASLILR
                     ENKRYYLNFPTLESLDSLELDQEIFVREASPVYQALLEQSFETELRNQINAAILVEKT
                     DFARIKMTLSNYFYKVKQQYPLTEKQQELYDILGDVNPEYALKYMTAFLLKFLKKDQL
                     MQKCRDIFVDSLVVLGYIVQNEDGKYELAIDFDKERLTFYLA"
     gene            43399..43581
                     /locus_tag="VVL45_000136"
     CDS             43399..43581
                     /locus_tag="VVL45_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU4186398.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGLKNVFTK"
     gene            complement(43627..45072)
                     /locus_tag="VVL45_000137"
     CDS             complement(43627..45072)
                     /locus_tag="VVL45_000137"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEU4186399.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVVFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVDIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTATYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQSYLVKRVYGLTFDVGVFLNISPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGVDCYQIIQGKKESYPGDTAVAENYL"
     gene            45178..46800
                     /locus_tag="VVL45_000138"
     CDS             45178..46800
                     /locus_tag="VVL45_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEU4186400.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            47003..48961
                     /locus_tag="VVL45_000139"
     CDS             47003..48961
                     /locus_tag="VVL45_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU4186401.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGVLQNLGLEPGEANDKAKEVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            49133..49366
                     /locus_tag="VVL45_000140"
     CDS             49133..49366
                     /locus_tag="VVL45_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEU4186402.1"
                     /translation="MKLKLLRVDTKVIMGSFFLVLSSLLALLLPLILKGLIDGSSIEN
                     IGSKVFQSFLIFIGQALFSSIGYYLFSQSGEKR"
     gene            49372..50814
                     /locus_tag="VVL45_000141"
     CDS             49372..50814
                     /locus_tag="VVL45_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4186403.1"
                     /translation="MRKKVIEGLIYAEKSFFDKSQSGELTSAIVNDTSVIREFLITTF
                     PNIILSLVMVLGSIVVLFSLDWNLSLLLFITLPCMMFIILPLSNISEKYSRRLQEEIG
                     FLTGQLTEKIQEHELIKTNQAEKSVQDVLDNCIERVQNNSLKSDRITSFETPFALLFI
                     FATIAVMLTYGGYRVSAGYISVGTLVSFLIYLFQLLNPISNIANFVTVYSRSKGSSVA
                     LENLLAVPKEKFEGGKSVSGRGLNFNHVYFGYDENRPVLKDIICSIFKGQKIAFVGPS
                     GSGKSTIVRLLERFYKPLSGDILMEQSSIYDFNLKEWRSKIAWVSQNNAVLSGSIRDN
                     LCLGLNRLVTDDELMKVLDLVSLGDEIRSMKEGLDTEVGERGRLLSGGQSQRLQIARA
                     YLKDAEILIFDEATANLDADSEYAIISSLYSVLKEKTVVIIAHRLSTVKDVDCIFFLE
                     EGKITGSGTHKELLENHERYARFVQEQMIE"
     gene            50972..52066
                     /locus_tag="VVL45_000142"
     CDS             50972..52066
                     /locus_tag="VVL45_000142"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU4186404.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGHFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGSKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQALEG"
     gene            52074..53240
                     /locus_tag="VVL45_000143"
     CDS             52074..53240
                     /locus_tag="VVL45_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU4186405.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDSLFEIDFDQLEKDLVEEE
                     VKLYVLCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKC"
     gene            53333..56431
                     /locus_tag="VVL45_000144"
     CDS             53333..56431
                     /locus_tag="VVL45_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU4186406.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDFFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLDRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVPDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVDRQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQDIAQEEIDQAIQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPLQATQVKTDLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILADNHQIYVTSY
                     ATFRQDSELYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVIYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            56479..57096
                     /locus_tag="VVL45_000145"
     CDS             56479..57096
                     /locus_tag="VVL45_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU4186407.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPIAEEKPVKPIEKQVGKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            57107..58441
                     /locus_tag="VVL45_000146"
     CDS             57107..58441
                     /locus_tag="VVL45_000146"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEU4186408.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLYQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFSGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            58451..58915
                     /locus_tag="VVL45_000147"
     CDS             58451..58915
                     /locus_tag="VVL45_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4186409.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQYLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     ELDYKGIRLESPDELLSYFEMNGFVDEEATLLYATSQGYSMIWFNPFYLEEQ"
     gene            58912..59406
                     /locus_tag="VVL45_000148"
     CDS             58912..59406
                     /locus_tag="VVL45_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4186410.1"
                     /translation="MKIRKARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            59487..61142
                     /gene="mltG"
                     /locus_tag="VVL45_000149"
     CDS             59487..61142
                     /gene="mltG"
                     /locus_tag="VVL45_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEU4186411.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSTEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDI
                     IRDTSRRSRREGAKPVKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQAVGQLQGDFKESLTAETF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            61213..61695
                     /gene="greA"
                     /locus_tag="VVL45_000150"
     CDS             61213..61695
                     /gene="greA"
                     /locus_tag="VVL45_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEU4186412.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            62203..62601
                     /locus_tag="VVL45_000151"
     CDS             62203..62601
                     /locus_tag="VVL45_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4186413.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
     gene            63046..64053
                     /locus_tag="VVL45_000152"
     CDS             63046..64053
                     /locus_tag="VVL45_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU4186414.1"
                     /translation="MQDNYTTKAKHLTIDSRRLIERWKKEGKSNREIASLLGKVPQTI
                     HTEIKRGTVRQCLGKGRFKEVYSADYAQQSYENNRKRSVKKSSLTKELKEKILHYHNQ
                     KFSPEMMVMAKGVNVGISTIYYWIHHGKLGLSKQDLLYPRKGKALKKQASTNFKPAGQ
                     SIEQRSEAINLRLENGHYEIDTVLLTRAKNYCLLVLTDRKSRHQIIRLIPNKSAEVVN
                     QALKLILKQHKILSITADNGTEFNRLFDVFSEEHIYYAHPYASWERGTNENHNRLIRR
                     WLPKGTKKMTPKEVAFIEKWINNYPKKCLDYKSPREDFWMANLNLKFSKMEIIFIKRF
                     Q"
     gene            64128..64328
                     /locus_tag="VVL45_000153"
     CDS             64128..64328
                     /locus_tag="VVL45_000153"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEU4186415.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
     gene            64363..65079
                     /gene="atpB"
                     /locus_tag="VVL45_000154"
     CDS             64363..65079
                     /gene="atpB"
                     /locus_tag="VVL45_000154"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEU4186416.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            65093..65587
                     /gene="atpF"
                     /locus_tag="VVL45_000155"
     CDS             65093..65587
                     /gene="atpF"
                     /locus_tag="VVL45_000155"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEU4186417.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            65587..66123
                     /locus_tag="VVL45_000156"
     CDS             65587..66123
                     /locus_tag="VVL45_000156"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEU4186418.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            66138..67643
                     /locus_tag="VVL45_000157"
     CDS             66138..67643
                     /locus_tag="VVL45_000157"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEU4186419.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            67659..68537
                     /locus_tag="VVL45_000158"
     CDS             67659..68537
                     /locus_tag="VVL45_000158"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEU4186420.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            68623..70029
                     /gene="atpD"
                     /locus_tag="VVL45_000159"
     CDS             68623..70029
                     /gene="atpD"
                     /locus_tag="VVL45_000159"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEU4186421.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFAGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            70040..70459
                     /locus_tag="VVL45_000160"
     CDS             70040..70459
                     /locus_tag="VVL45_000160"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEU4186422.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(70504..71517)
                     /locus_tag="VVL45_000161"
     CDS             complement(70504..71517)
                     /locus_tag="VVL45_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEU4186423.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FERMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
     gene            71661..72827
                     /locus_tag="VVL45_000162"
     CDS             71661..72827
                     /locus_tag="VVL45_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU4186424.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            72817..74046
                     /locus_tag="VVL45_000163"
     CDS             72817..74046
                     /locus_tag="VVL45_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU4186425.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLAL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILELQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEYYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
BASE COUNT        22041 a        13772 c        16731 g        21507 t
ORIGIN      
        1 gctccataat atccataggg gatttaccca ctacaaatat tatagagccc gaaaatatgg
       61 gaaaactgat ccttgtttct gcttttgtct atagaagaat aataaagatt atcttcttca
      121 aattctccga tattctctaa agttttgtgc aagttgcaca gaacttgttt atttttttgg
      181 tcatcttgcc atagaaatat aaagcgtttt catatataat ataattatca aaagacaaaa
      241 ggagttcacc tcatggtaga attgaatctt aaaaatattt acaaaaaata tccaaacagc
      301 gaacactatt cagttgaaga tttcaacttg aacatcaaag ataaagaatt tatcgttttc
      361 gtaggacctt caggatgtgg taaatcaact acactccgta tgattgctgg tcttgaagac
      421 attacagaag gtactgcatc tatcgatggc gtagttgtca acgacgtagc tccaaaagac
      481 cgtgatatcg ccatggtatt ccaaaactac gctctttacc cacacatgac tgtttatgac
      541 aacatggctt tcggtttgaa attgcgtaaa tacagcaaag aagacattaa caaacgtgtt
      601 caagaagcag ctgaaatact tggattgaaa gaattcttgg aacgtaaacc agctgacctt
      661 tcaggtggtc aacgtcaacg tgttgccatg gggcgtgcga ttgtccgtga tgcgaaagta
      721 ttcttgatgg acgaaccttt gtcaaacttg gatgccaaac ttcgtgtatc aatgcgtgct
      781 gaaatcgcta aaattcaccg tcgtatcgga gctacaacta tctatgtaac tcacgaccaa
      841 acagaagcga tgacacttgc agaccgtatc gttattatgt cagctactaa gaaccctgct
      901 ggtacaggta ctatcggacg tgtagaacaa atcggtactc ctcaagaagt ttacaaaaat
      961 ccagttaaca aattcgttgc aggattcatc ggaagcccag ctatgaactt catcaccgtg
     1021 aaattggttg gtagcgaaat tgtttctgac ggtttccgtt tgaaagtgcc agaaggagca
     1081 ttgaaagttc ttcgtgaaaa aggctacgaa ggaaaagaat tgatctttgg tatccgtcca
     1141 gaagacgtga atgcagaacc tgctttcctt gaaacattcc cagactgtgt tgtaaaagcg
     1201 actatctctg tatcagaact gcttggttca gaatctcacc tttactgtca agttggtaaa
     1261 gacgagtttg ttgcaaaagt tgatgctcgt gactacttgc aaacaggtgc aacagttgag
     1321 cttggatttg acttgaacaa agcacacttc ttcgatgtag aaactgaaaa aacaatctac
     1381 taaaataaat aaaattcaaa gcactacaag aaaagatatc tctttatcaa ttgtagtgga
     1441 gagatatcag ttaatctagg gagagaaaca aaatgcttct ctcctttttg ctagagaagt
     1501 catattatgc atctatattg tgatgctctt taatactctt cgaaaatctc ttcaaaccac
     1561 gtcaacgtcg ccttgccgta cgtatgatta ctgatttcgt cagttttatc tgcaacctca
     1621 aagatgtact ttgagcagct tacggctagt ttcctagttt gctctttgat ttccattgag
     1681 tattatttgt gggtaccatc tacaagtgaa gctatatgcg taaactacgt gagcaattga
     1741 attcgaacta gagaggtaat aataaattta tgctatagtt atggtgactt gtatgctttt
     1801 gattctagtt tatcaaataa tagattagaa ttgtcagata atatcatttt gtgttataat
     1861 gaagaaaaaa cagaggtgtt caaatgtcag aagcaggtca taagttttta gcaaaattgg
     1921 ggaaaaaacg cttacgtcca ggtggaaagc gtgccacaga ttggttaatt gcagaaggag
     1981 gattttcaaa agaaaagaga atactagagg ttgcgtgtaa taggggaact acagcaattg
     2041 agttggcaca gcgttttggt tgcaagataa ctgctgttga tatggatgct caagctttag
     2101 aagtggctaa aaaatctgct ggaacggcag gtgttgctca tttaatcagt tttgaaagag
     2161 caaatgcaat gaaacttcct tatcaagatg ctagttttga tattgttata aatgaagcta
     2221 tgctgactat gcaagccgat caagctaaga aaaaatgtgt aatggaatat ctaagggtat
     2281 taaaacctgg aggtcttctc ttgacacatg atgtgcttct taaggaagct aaagagtcta
     2341 tcagacagga attatcacaa gcaattcatg taaatgtagg tcctttaact caagatggtt
     2401 gggaacaggt gatgatagaa tcaggttatt gtgatgtgaa agcattgact ggtgaaatga
     2461 cattaatgaa attatcgggt atgatttatg acgaaggttt gctaggaact ttgaaaattt
     2521 gtgtaaatgc ttgtaaaaag gagaatagaa agcagttttt aactatgtat aaaatgtttg
     2581 ctaagaataa acagaaattg ggctttattg cgatggctag ttataaatcg tcaaaacgtt
     2641 agataattat tgaagttaac ttttcctttt ttctttctta aaaaatatgc tataatagag
     2701 agtaaaaaac tttgaaagaa agaaaaagat gaatttaaaa gattacattg caacaattga
     2761 aaattatcca aaggaaggca ttaccttccg tgatattagt cctttgatgg ctgatggaaa
     2821 tgcttatagc tacgctgttc gtgaaatcgt tcagtatgct actgacaaga aagtcgacat
     2881 gatcgtggga cctgaagctc gtggatttat cgtgggttgt ccagttgcct ttgagttggg
     2941 aattggtttt gcgcctgttc gtaagccagg taaattgcca cgcgaagtta tttctgctga
     3001 ctatgaaaaa gagtacggtg tcgatacctt gactatgcac gcggatgcca ttaagccagg
     3061 tcaacgtgtt cttattgtag atgacctttt ggcgacaggt ggaactgtta aggcaactat
     3121 cgagatgatt gaaaaacttg gtggtgttat ggcaggttgt gccttccttg ttgaattgga
     3181 tgaattgaac ggccgtgaaa aaattggtga ctacgactac aaagttctta tgcattatta
     3241 atgaaaacag tccctagggc tgttttctct acactaggat ataaaaatag actataacta
     3301 gttagagaaa aactataatt gaaaactata tcttcttgca gtataataaa aggactaagt
     3361 gtttgagatt tgtcttcaaa catatgcaat tattcctgaa agagtacagt taggagaggg
     3421 ttatgccgat tcgaattgat aaaaaattgc cagctgttga gattttacgg acagagaata
     3481 tctttgtcat ggatgatcaa cgtgctgccc accaagatat ccgtcctttg aagattttaa
     3541 ttttaaatct catgccacag aaaatggtca cagagaccca gttgttgcgc cacttggcta
     3601 atacacccct acaactggat attgattttc tctatatgga gagccaccgt tctaaaacaa
     3661 ctcgttcaga gcacatggag accttctata aaacttttcc tgaagtcaag gatgagtatt
     3721 ttgatgggat gatcatcacg ggtgctccag ttgagcattt accatttgag gaagtggact
     3781 attgggagga atttagacag atgcttgagt ggtctaagac tcatgtctat tcgacccttc
     3841 atatctgttg gggggctcag gctgggcttt atctgcgcta tggtgtagaa aaataccaga
     3901 tggacagtaa gctatcaggt atttatcctc aggacaccct aaaagagggt caccttctat
     3961 ttagaggctt tgatgatagc tatgtatccc ctcattcacg gcacacggag atttctaagg
     4021 aagaggtctt aaacaagacc aatctcgaga ttttatcaga aggacctcag gttggggttt
     4081 ctattttggc cagtcgtgat ttacgagaaa tttatagttt tggtcatttg gagtatgacc
     4141 gtgatacctt ggcaaaagag tattttcgag atcgtgatgc aggttttgac ccacatattc
     4201 cagaaaatta ctttaaagat gatgatgtta atcaggtacc ttgtctttgt tggtcttcat
     4261 ctgcagccct ctttttcagt aattgggtaa accatgcggt ctatcaggag acgccttttg
     4321 attggagaaa gatagaagat gatgcatctg catatgggta tttataagag gaattatgac
     4381 atatttagac gcttttaaat caggtacctt ggttttaccg agtgccctgc tcttgcattt
     4441 taaggaactc tttccttcta gcgacgattt tctggtttgg caatttttct atttgcaaaa
     4501 tacgacaggc ttagaagaaa tgtcgccaag ccagattgct gaaaggattg gcaaggaaat
     4561 ttcggatgtc aaccagtcca tttctaatct gacggaaagg ggactgctcc agtatcgtac
     4621 tatcgaatta aatggcgaaa ttgaattgct ctttgatgct agtttggcct tggaacgttt
     4681 ggatgacctg tttggagcag ttcattcaag ttcagaccag ctaacacctc aaaaccagct
     4741 caaggatttg gtggaaacct tccagcagga gttgggacga ttgttgacgc cttttgagat
     4801 tgaggatttg accaagacac taaaggaaga tggaaccagt gctgacttga ttaaggaggc
     4861 tcttcgtgaa gctgttttga atggaaaacc aaactggaag tacattcagg cgattttgag
     4921 aaactggcgc catgaaggaa tcaagagtgt ggctcaaatt gaggccaaga gagcagaaag
     4981 agaagcaagc aatcctcagt tgacacaggt atctgcagat ttcataaatg ccatggatct
     5041 ctggaaggat taatccatgc aagtaggctt gaaatccgag taagatttgc aagctgtgta
     5101 taattgtgat agaataaata gaaaataaat tgaaaaaaga ggtatgtgaa atgtcacgta
     5161 aaccatttat cgctggtaac tggaaaatga acaaaaatcc agaagaagct aaagcattcg
     5221 ttgaagcagt tgcatcaaaa cttccttcat cagatcttgt tgaagcaggt atcgctgctc
     5281 cagctcttga tttgacaact gttcttgctg ttgcaaaagg ctcaaacctt aaagttgctg
     5341 ctcaaaactg ctactttgaa aatgcaggtg ctttcactgg tgaaactagc ccacaagttt
     5401 tgaaagaaat cggtactgac tacgttgtta tcggtcactc agaacgccgt gactacttcc
     5461 atgaaactga tgaagatatc aacaaaaaag caaaagcaat ctttgcgaac ggtatgcttc
     5521 caatcatctg ttgtggcgaa tcacttgaaa cttacgaagc tggtaaagct gctgaattcg
     5581 taggtgctca agtatctgct gcattggctg gattgactgc tgaacaagtt gctgcctcag
     5641 ttatcgctta tgagccaatc tgggctatcg gtactggtaa atcagcttca caagatgatg
     5701 cacaaaaaat gtgtaaagtt gttcgtgacg ttgtagctgc tgactttggt caagaagtcg
     5761 cagacaaagt tcgtgttcaa tacggtggtt ctgttaaacc tgaaaatgtt gcttcataca
     5821 tggcttgccc agacgttgac ggtgcccttg taggtggtgc gtcacttgaa gctgaaagct
     5881 tcttggcttt gcttgacttt gtaaaataat cagtaagtag caaaagctag gtggaacagc
     5941 attcagatgt ctgttacatt ttttatagga gaggaaagat tgaaaacaaa aattggatta
     6001 gcaagtatct gtttactagg cttggcaact agtcatgtcg ctgcaaatga aactgaagta
     6061 gcaaaaactt cgcaggatac aacgacagct tcaagtagtt cagagcaaaa tcagtcttct
     6121 aataaaacgc aaacgagcac agaagtacag actaatgctg ctgcccactg gtatggggat
     6181 tattatgtaa aggatgatgg ctctaaagct caaagtgaat ggatttttga caactactat
     6241 aaggcctggt tttatattaa ttcagatggt cgctactcgc agaatgaatg gcatggaaat
     6301 tactacctga aatcaggtgg atatatggcc caaaacgagt ggatctatga cagtaattac
     6361 aagagttggt tttatctcaa gtcagatggg gcttatgctc atcaagaatg gcaattgatt
     6421 ggaaataagt ggtactactt caagaagtgg ggttacatgg ctaaaagcca atggcaagga
     6481 agttatttct tgaatggtca aggagctatg atgcaaaatg aatggctcta tgatccagcc
     6541 tattctgctt atttttatct aaaatccgat ggaacttatg ctaaccaaga gtggcaaaaa
     6601 gtgggcggca aatggtacta tttcaagaag tggggctata tggctcggaa tgagtggcaa
     6661 ggcaactact atttgactgg aagtggtgct atggcgactg acgaagtgat tatggatggt
     6721 gctcgctata tctttgcggc ctctggtgag ctcaaagaaa agaaagattt gaatgtcggc
     6781 tgggttcaca gagatggtaa acgctatttc tttaataata gagaagaaca agtgggaacc
     6841 gaacatgcta agaaaatcat tgatattagt gagcacaatg gtcgtatcaa tgattggaaa
     6901 aaggttattg atgagaacga ggtggatggt gtcattgttc gtctaggtta tagcggtaaa
     6961 gaagacaagg aattggcgca taacattaag gagttaaacc gtctgggaat tccttatggt
     7021 gtctatctct atacctatgc tgaaaatgag accgatgctg agaatgacgc taaacagacc
     7081 attgaactta taaagaaata caatatgaac ctgtcttacc ctatctatta tgatgttgag
     7141 aactgggaat atgtaaataa gagcaagaga gctccaagtg atacagacac ttgggttaaa
     7201 atcatcaaca agtacatgga cacgatgaag caggcgggtt atcaaaatgt gtatgtctat
     7261 agctatcgta gtttattaca gacgcgttta aaacacccag atattttaaa acatgtaaac
     7321 tgggtagcgg cctatacgaa tgctttagaa tgggaaaacc cttattattc aggagaaaaa
     7381 ggttggcaat atacctcttc tgaatacatg aaaggaatcc aagggcgcgt agatgtcagc
     7441 gtttggtatt aagcgatgat ttgaaagagg gatgtgatag tagcaccctc tttttctttg
     7501 ttttatgata gttcatcctc gagtaaattc aagatcttgc ttggaaatga agcttatata
     7561 gtagattgaa tatagacaaa taccttgtga ttggtaaaac attttagaaa ttcatttacc
     7621 tttcctaatc gacttggttt catcttattt caatttatta tagtattggg gaatttcttc
     7681 aaaccacatc agcttggtca gttctacctg cgacctcaaa acttgtgctt tggtcaaact
     7741 gggtttagtt tcctagtttg ctgatggatt tccattgact ataagcatcc aaccctcttt
     7801 ttgtcttcta aagaattctt aaattatcag tctattgcaa cttttctcat ataagttctt
     7861 tgtcttgcta ttggttttcc ttagtagtat actaaggtag taatcattaa gaagtggtta
     7921 caaaaaataa tgaatgaggt aaagaaaatg gtagaattga aaaaagaagc agtaaaagac
     7981 gtaacatcat tgacaaaagc agcgccagta gcattggcaa aaacaaagga agtcttgaac
     8041 caagctgttg ctgatttgta tgtagctcac gttgctttgc accaagtgca ctggtatatg
     8101 catggtcgtg gtttccttgt atggcatcca aaaatggatg agtacatgga agctcttgac
     8161 ggtcaattgg atgaaatcag tgaacgcttg attacactcg gtggaagccc attctctaca
     8221 ttgacagagt tccttcaaaa tagtgaaatc gaagaagaag ctggtgaata ccgtaatgtt
     8281 gaagaaagct tggaacgtgt tcttgttatc taccgttact tgtcagaact tttccaaaaa
     8341 ggtttggatg tcactgatga agaaggtgac gatgtgacaa acggtatctt tgcaggcgct
     8401 aaaactgaaa cagataaaac aatttggatg cttgcagccg aacttggaca agcacctggt
     8461 ttgtaagaaa taaggaaaca tagataaatc tgtaggaaat ttcttacaga ttttttctat
     8521 tttgtaagct attccaaacc agtctttttt tgagtttttt gataaaatag tactatcagt
     8581 gaaaaggatg gaagcatgac taagaaaatc gtagctattt gggcccagga tgaagagggt
     8641 gtgattggta aggaaaatcg tctgccttgg catttgccag cagaattgca gcactttaaa
     8701 gaaacaactc tgaatcatgc tatcttgatg gggcgtgtga cctttgatgg gatggggcgt
     8761 cgcttgcttc caaaacggga aaccttgatt ttgacgcgta atccggaaga aaagatagat
     8821 ggggttgcta cttttcagga cgtccagtct gttcttgact ggtatcaggc tcaagaaaag
     8881 aatctctaca ttatcggtgg gaagcaaatt tttcaggctt ttgaacctta ccttgatgaa
     8941 gtgattgtca ctcacattca tgctcgggtg gaaggagata cctatttccc tgaagaactt
     9001 gacttgtctc tttttgagac agtttcaagc aaattttacg ccaaagatga gaagaatcct
     9061 tatgatttta ccatccaata ccgcaagaga aaggaagtct aatggaacgg agtttatttg
     9121 gtctattcac ggcttttctg tgtttcatct gctttctggc aggagcacaa gcttttcgta
     9181 aaaagcgtta cgggctttct atcctccttt ggttaaatgc ctttaccaat ctggtcaata
     9241 gtattcatgc tttttacatg accttatttt agatagaatg acagtataga atggaaggaa
     9301 atcatgtcta caaatagaaa aaatgatatg atggtttatt gctcattttg tggcaaaaac
     9361 caagaagaag tacaaaaaat aattgctggc aacaatgctt ttatttgtaa tgaatgcgtg
     9421 gagttagctc aggaaatcat tcgagaagaa ttggttgagg aagtcttggc agacttgtct
     9481 gaggtgccaa aaccaattga actcctccat atcttgaacc actatgtaat tggtcaagat
     9541 cgtgccaagc gtgccttggc agtggcggtt tataaccact acaaacgcat caatttccac
     9601 gatacacgcg aagagtcaga agatgtggat ttgcagaagt caaacatttt gatgattggc
     9661 ccaactggtt cagggaaaac tttccttgcc cagaccttgg ctaagagctt gaatgtacct
     9721 tttgctattg cggatgcgac agctctgacg gaggctggtt atgtgggtga ggatgtggaa
     9781 aatatcctcc tcaaactctt gcaggttgct gactttaaca tcgaacgtgc agagcgtggc
     9841 attatctatg tggatgaaat tgacaagatt gccaagaaga gtgagaatgt gtctatcaca
     9901 cgtgatgttt ctggtgaagg ggtgcaacaa gcccttctca agattatcga gggaactgtt
     9961 gctagcgtgc cgcctcaagg tggacgcaaa catccacaac aagagatgat tcaagtggat
    10021 acaaaaaata tcctcttcat cgtgggtggt gcttttgatg gtattgaaga aattgtcaaa
    10081 caacgtctgg gtgaaaaagt catcggattt ggtcaaaaca ataaggcgat tgacgaaaac
    10141 agctcataca tgcaagaaat catcgctgaa gacattcaaa aatttggtat tatccctgag
    10201 ttgattggac gcttgcctgt ttttgcggct cttgagcaat tgaccgttga tgacttggtt
    10261 cgcatcttga aagagccaag aaatgccttg gtgaaacaat accaaacctt gctttcttat
    10321 gatgatgttg agttggaatt tgacgacgaa gcccttcaag agattgctaa taaagcaatc
    10381 gaacggaaga caggggcgcg tggacttcgc tccattatcg aagaaaccat gctagatgtt
    10441 atgtttgagg tgccgagtca ggaaaatgtg aaattggttc gcatcactaa agaaactgtt
    10501 gatggaacgg ataaaccgat cctagaaaca gcctagaggt gactatggaa cttaatacac
    10561 acaatgctga aatcttgctc agtgcagcta ataaatccca ctatccgcag gatgaactgc
    10621 cagagattgc cctagcaggg cgttcaaatg ttggtaaatc cagctttatc aacactatgt
    10681 tgaaccgtaa gaatctcgcc cgtacatcag gaaaacctgg taaaacccag ctcctgaact
    10741 tttttaacat tgatgacaag atgcgctttg tggatgtgcc tggttatggc tatgctcgtg
    10801 tttctaaaaa ggaacgtgaa aagtgggggt gcatgattga ggagtactta acgactcggg
    10861 aaaatctccg tgcggttgtc agtctagttg accttcgtca tgacccgtca gcagatgatg
    10921 tgcagatgta cgaatttctc aagtattatg agattccagt catcattgtg gcgaccaagg
    10981 cggacaagat tcctcgtggt aaatggaaca agcatgaatc agcaatcaaa aagaaattaa
    11041 actttgaccc aagtgacgat ttcatcctct tttcatctgt cagcaaggca gggatggatg
    11101 aggcttggga tgcaatctta gaaaaattgt gaggaaaaga aaatggcaaa aacaattcat
    11161 acagataagg ccccaaaggc tatcgggccc tatgttcaag gaaaaatcgt tggcaacctt
    11221 ttgtttgcta gcggtcaagt tcccctatcc cctgaaactg gggaaattgt aggagagaat
    11281 atccaagaac agacagagca agtcttgaaa aacatcggtg ctattttggc agaagcagga
    11341 acagactttg accatgttgt caaaacaact tgtttcttga gcgatatgaa cgactttgtt
    11401 ccttttaatg aggtttacca aacggccttc aaagaggaat tcccagctcg ttcagctgtg
    11461 gaggtagctc gtcttcctcg tgatgtaaaa gtcgaaattg aagtcatcgc agagattgga
    11521 taagctagtt gaagtttggt gttgccaaac ttcttttgat ataaggagaa aaagatgaca
    11581 aagaaacaac ttcacttggt gattgtgaca gggatgagtg gcgcagggaa aactgtagcc
    11641 attcagtcct tcgaggatct aggttatttc accattgata atatgccgcc agctctcttg
    11701 cctaagtttt tgcagctggt tgaaattaag gaagacaatc ctaagttggc cttggtagtg
    11761 gatatgcgta gccgttcttt cttttcagag attcaagctg ttttggatga gttggaaaat
    11821 caagatggtt tggatttcaa aatcctcttt ttggatgcgg ctgataagga attggtcgct
    11881 cgttacaagg aaaccagacg gagtcaccca ctagcagcag acggtcgtat tttagatgga
    11941 atcaagttgg aacgtgaact cttggcacct ttgaaaaata tgagccaaaa tgtggtggat
    12001 acgactgaac tcactccacg tgagctgcgc aaaacccttg cagagcagtt ttcagaccaa
    12061 gaacaagccc agtctttccg tatcgaagtc atgtctttcg gatttaagta tggaatcccg
    12121 attgatgcgg acttggtctt tgatgtccgt ttcttgccaa atccctatta tttaccagaa
    12181 ctgagaaacc aaacgggtgt ggatgaacct gtttatgatt atgtcatgaa ccatcctgag
    12241 tcagaagact tttatcaaca tttattggcc ttgattgagc cgattctgcc aagttaccaa
    12301 aaggaaggta agtccgtttt gaccattgcc atgggatgta cgggtggaca acaccgtagt
    12361 gtggcatttg ctaaatgctt ggcgcaggac ttatccaaga attggtctgt taatgaaggg
    12421 catcgcgaca aagaccgcag aaaggaaacg gtaaaccgtt catgagaaaa ccaaagataa
    12481 cggtgattgg tggagggact ggaattcccg tcattctaaa aagtctgcgg gaaaaagatg
    12541 tggaaatcgc agctatcgtg acggtggcag atgatggtgg ttcttcaggt gaactccgaa
    12601 aaaatatgca acagttgaca ccgccaggtg atcttcgtaa tgtccttgtg gccatgtcgg
    12661 atatgcctaa gttttatgag aaggtctttc agtatcggtt ctctgaggat gccggagcct
    12721 ttgctggcca tccattggga aatctcatca ttgctggctt gtcagaaatg cagggttcaa
    12781 cctataatgc catgcagtta ttgagcaaat ttttccatac aacagggaaa atttatcctt
    12841 ccagtgacca tcctttgacc cttcatgcag tctttcagga tgggacagaa gtggctggag
    12901 agagtcatat tgtagaccat cgaggcataa ttgacaatgt ctatgtgacc aatgccctaa
    12961 acgatgatac gcctctggcc agccgtcgag tagtgcagac catccttgaa agtgacatga
    13021 ttgtcctagg gccaggttcc ctctttacct ctattttgcc caatatcgtg attaaggaaa
    13081 ttgggcgggc tcttttggaa actaaggcag aaattgccta tgtctgcaat atcatgaccc
    13141 aacgtgggga gacggaacac tttacagata gcgaccacgt ggaagtcttg catcgtcacc
    13201 ttggtcgccc ttttatcgac actgtcttgg tgaatattga aaaagtgcct caggaataca
    13261 tgaattccaa ccgttttgat gaatacttag tgcaagtgga acacgatttt gtaggtcttt
    13321 gtaagcaagt ttcgcgcgtg atttcatcta acttccttcg tctggaaaat ggcggtgcct
    13381 tccacgatgg agatttgatt gtggacgagt tgatgcgcat tatacaggtg aaaaaatgag
    13441 tttcacagta gcagtaaaag aagaaatcct gggccaacac cacctgagcc ggcatgaatt
    13501 atctgccatt atcaaaatgt ctggtagcat cggtctctcg acttcgggct tgactttgtc
    13561 agttgtgaca gaaaatgcca aactggcccg tcacctctat gagtcctttc tccatttcta
    13621 tgaaatcaaa tctgaaattc gtcactacca acggagcaat cttcgcaaga atcgcgtcta
    13681 taccgttttt acagatgaaa aggtgcagga tttgttaagt gatttgcact tggcagactc
    13741 tttctttggc ctggaaacag gtattgatga ggcgatttta tcggacgagg aagcaggtcg
    13801 tgcctatctc tgtggcgctt tcttggcaaa tgggagcatt cgtgaccctg agtcaggcaa
    13861 gtaccagttg gaaatcagtt ctgtttatct ggaccacgcg caaggaattg cctcccttct
    13921 ccaacagttt ttactggatg ccaaggtgct tgagcgcaag aagggggctg tgacctatct
    13981 ccagcgagca gaagacatta tggacttctt gatagtaatc ggagccatgc aggcacgtga
    14041 tgattttgag cgggttaaga ttttgcgaga aacccgtaac gacctcaatc gggctaataa
    14101 tgccgagaca gctaatatcg ctcggacagt ttctgccagc atgaagacca ttaacaatat
    14161 cagtaaaatc aaagatatca tgggcttaga aaatttgcca gtagatttgc aggaagtggc
    14221 gcaactgagg attcagcacc cagactactc tatccagcag ttagcagata gtctcagcac
    14281 ccctctgacc aaaagtggtg tcaatcacag actcagaaaa atcaataaga tagcggatga
    14341 attataaaac cacgaatcct atgtgactcg tggttctttt ttataaacta gtagagtgtt
    14401 ttggttgtac tttttgttca gggtcaatgt agttgatagc gttgttgaca gcagttggag
    14461 cttctccgag gcctgtcgca atcagatcaa tttttccgtc atagtagcag cagtcaccga
    14521 tagcatagat acctgcttgg ctggattcct gtttgctgtt gacgataatc ttgtgacggt
    14581 tgaggtcgag cccccagttt ttaaggttac cgacagaaga tttgaaacca tagttgacaa
    14641 agaggtggtc taggtcaatg gtttcagttt catcagattt gacttttgtg atttcaagtt
    14701 tatcaagtgt ttttccattt ccaaggagtt ggctaggggc gaatggtgtc ttgatggtta
    14761 cagatgattc ttgcaaggct tgaacactgt gttccaaggc acggaaatta tctctgcggt
    14821 gaacaagggt agttggtgcg attttttcaa aagccaaagc ccaatccaca gccgagtctc
    14881 ccccaccaag aatcgtcact ttcttaccag cgtattgctg aatgttagaa acgtggtagt
    14941 ggatattttc atagccctca accccttcaa gttccagcgg acgtggtttg aaggcaccgc
    15001 cacccatagc gatgataact gttttagtca ggtgacttcc tttagaagtt gtgatggcaa
    15061 attcttcttg tttgtcaatc tcaagaaccg tttcattgag atgaataggg gtatcaaatc
    15121 catttagctg ttcaatcaag cggttagtca actcttctcc agtcaggttt gggaagcctg
    15181 gtacgtctag gatttccttt tcagggtaga gaatagcagg ttgtccacct agctggggaa
    15241 gagagtcgat gatttgaacc ttggcttggc gtaggtgggc ataaaaggct gcaaaaagcc
    15301 cgacaggacc accacccaca atggtaatat catagagttg agacatagtt tctcctttgt
    15361 tttttctagt cagtttattt tatcatattt ttgttcaatg taaaatggac taggataggg
    15421 aaaaaaatgc cagaaaatgg tataatagga cgaaagtgtt ttgataggga ggatatcagg
    15481 gatgaatttt caacaattat ccaatctgca atattggacc agtttgtttg caagtccatg
    15541 gacgatagct atcaatctga ttgatatttt gattgttgct tatattttat accattttac
    15601 aaaagctatt gcaggaacca agattatgat tttggtacgt ggagttttgg tgtttatttt
    15661 agctcaaatc cttgcaaata tgattggttt gactacgatt tcttggttaa tcaatcaaat
    15721 tattacttat ggggttattg cggcggttgt tatcttctct ccagagattc ggactggttt
    15781 ggaacgtttg ggaagagcga cagatttttt ttccaatgcc cctattagtg ctgaggaaca
    15841 gatgattcgt gcctttgtta agtctgttga atacatgagt cctcgtaaaa tcggggcctt
    15901 ggttgctatt cagcgtgtac gtaccttgca ggagtatatt tcgacaggaa ttcccttgga
    15961 tgctaagatt tctgcagaac ttctcattaa catttttatt cccaacactc ccctacatga
    16021 tggtgcggtg attatcaaag aagaacgtat cgctgtgacg tctgcctatc tgcccttgac
    16081 aaaaaacaca gggatttcca aggaatttgg gaccagacac cgggcggcta tcggtttatc
    16141 agaagtctca gatgccttga cttttgtcgt atcagaggaa acgggaggaa tttcgataac
    16201 ctataatgga aggtttaagc acaacctaac acttgatgaa tttgaaacag aattacgtga
    16261 aatcttactt ccaaaagagg aagtgggtct tagttttaaa gaacgattgc taggaggatg
    16321 gaaacatgaa aaaaaatagt ttatatatca tatcctcact cttttttgct tgtgtcttat
    16381 ttgtctatgc tacggcgacg aattttcaaa acagtaccag tgctaggcag gtaaaaacgg
    16441 aaacctatac taatacagta acaaatgtcc ctattgacat acgctataat agtgataagt
    16501 attttattag cggttttgct tcagaagtat cagtggtctt gactggggca aatcgcctat
    16561 cgctagctag tgaaatgcaa gaaagtacac gtaaattcaa ggttactgct gacctaacag
    16621 atgccggtgt tggaacgatt gaagttcctt tgagcattga agatttaccc aatgggctga
    16681 ccgctgtagc gactccgcaa aaaattacag tcaagattgg taagaaggct cagaaggata
    16741 aggtaaagat tgtaccagag attgacccta gtcaaattga tagtcgggta caaattgaaa
    16801 atgtcatggt gtcagataaa gaagtgtcta ttacgagtga ccaagagaca ttggatagaa
    16861 ttgataagat tatcgctgtc ttgccaacta gcgaacgcat aacaggtaat tacagtggtt
    16921 cagtaccttt gcaggcaatc gaccgcaatg gtgttgtctt accggcagtt atcactccgt
    16981 ttgatacaat aatgaaggtg actacaaaac cagtagcacc aagttcaagc acatcaaatt
    17041 caagtacaag cagttcatcg gagacatctt cgtcaacgaa agcaactagt tcaaaaacga
    17101 attaaaaata gaaaaggatt ttataacaat gggtaaatat tttgggactg atggagtccg
    17161 tggagaagct aacctagaac taacaccaga attagccttt aaactaggac gttttggagg
    17221 ctatgttctt agtcaacatg aaacggaagc gccgaaagtc tttgtaggac gtgacacacg
    17281 tatttcaggg gaaatgctgg aatcggcctt ggtggcaggt ctcctttcag tagggattca
    17341 cgtatacaaa cttggtgtcc ttgcaacacc agcagtagct tacttggttg aaactgaagg
    17401 agcaagtgcc ggtgtcatga tttctgctag ccacaaccca gcccttgata acggaatcaa
    17461 gttctttggc ggtgatggct tcaaactaga tgatgaaaaa gaagcagaaa ttgaagcctt
    17521 gctagatgct gaggaagaca ctcttcctcg tccaagtgca gaaggcttag gaattttggt
    17581 agattatcca gaaggcttgc gtaagtatga aggatacctt gtttcaactg gaactcctct
    17641 tgatggaatg aaggttgcct tggatacagc taatggagca gcttctacca gtgcccgtca
    17701 aatctttgca gaccttggtg cccaattgac ggttatcggg gaaacaccag acggtcttaa
    17761 catcaacctt aatgttggtt caacacatcc agaagccctt caagaagtgg tcaaagaaag
    17821 tgggtcagct attggtttgg cctttgatgg agacagtgac cgcttgattg ctgttgatga
    17881 gaatggtgac atcgtcgatg gtgacaagat tatgtacatc atcggaaaat acctttctga
    17941 aaaaggacaa ttggctcaaa atacaattgt gacaactgtt atgtctaacc ttggtttcca
    18001 caaggccttg aatcgcgaag gtattaacaa ggcagttact gcagttggtg accgctacgt
    18061 tgttgaagaa atgagaaaat caggctacaa ccttggtggt gaacagtctg gtcacgttat
    18121 cttgatggat tacaatacca caggtgatgg tcaattatca gcagttcaat tgactaaaat
    18181 catgaaggaa actggtaaga gcttatcaga gttggcggca gaagtaacga tttatccaca
    18241 aaaattagtt aatatccgag tggaaaacgt catgaaggaa aaggccatgg aagtgccagc
    18301 tatcaaggcc atcatcgaga agatggaaga agaaatggcg gggaacggcc gtatccttgt
    18361 tcgtccaagt ggaacagagc ccctcttgcg tgttatggca gaagcgccta caacagaaga
    18421 agtggactac tatgttgata ccatcacaga tgtagttcgt gctgaaattg ggattgacta
    18481 aatcctagaa aactagatga aaataaaaaa ttatggtaca atagcttata atattgtagc
    18541 cgatggagaa agacatgaat cttaaacgtg aacaagaatt tgttagtcag tatcattttg
    18601 atgcgcgtaa ttttgaatgg gaaaatgaaa atggagctcc tgaaaccaag gtggatgtga
    18661 atttccaatt gctccaacat gaccaagaaa atcaagtgac ttccttgatt gtcatcttga
    18721 gctttatgat tgtatttgat aagtttgtca ttagtggaac aatctcacaa gtaaaccata
    18781 tcgatggccg tattgtcaat gaaccaagtg aattgaacca agaagaggtg gaaaccttgg
    18841 ctcgtccatg tttgaacatg ctcaatcgtt tgacttacga agtgactgaa attgcattag
    18901 acttaccagg aattaatttg gagttctaat atgaaattag ctgttttcac agattcctct
    18961 gcttatctca gtgaagagac cttgcaaaga gaagacttgt ttgtcttgga tattcctgtc
    19021 aatattgatg gtgaggaata tgtcgaaggc atcaatctgt ctgctgagga attttaccaa
    19081 aaaatggctc aggcttctga attgcctaag accagtcaac caagtattgc caagttagat
    19141 gagatcttaa cttcgctcaa agaacaaggc tatacacatg ccttggggct tttcctatct
    19201 tctggaattt caggttttta ccaaaatatc cagtatatgg tcgatgacta tgagggctta
    19261 accattgctt tcccagacac tttgattaca agtgctcccc taggcatcat ggttgaaagc
    19321 gtctttaatt ggcgtgatca gggcgatgat tttgccagca ttcaggataa gctagccatt
    19381 caaatcagcc gtacgtcagc ctttatcatg gtagatgact tggatcattt ggtaaaaggt
    19441 ggacgccttt caaatggggc tgccattttg ggcaatttgc ttagcattaa gccaatcctt
    19501 tattttaacg atcaaggtgt gattgaagtt tacgaaaaag ttcgtactga aaagaaggcc
    19561 accaaacgct taattgaaat tatcaaggaa acaacggctt caggtcaata ccgggtcatt
    19621 gtcattcatg ggaatgatcc tgaaaaggct gaagaattgc gtcagcattt gcttgatttt
    19681 ggcttgggtt cggatgtttc acttgctaca tttggtagtg tcattggaac gcacctagga
    19741 gcaggaagta ttgctctggg ttatattcca gtgatttagt aggcacttct aggctagtta
    19801 atcttagcaa ttgaacacgg actacctgcc tcttgaaaaa agatgtctgt cttgcctttc
    19861 gtggaaagtc agtgccattc cctatttttc atgggtggct aagatccttt gtattttgat
    19921 aattgaacac gcctggaacc ctgtgtgaaa aagatagttc ttccaaggag tagacacttc
    19981 ttgatcagaa ttcctatttt cactttgtgt tcttacaggc tttgtatctt agacaggagt
    20041 agagatgagt attcgagtaa ttattgccgg ttttaaggga aagatgggtc aagctgcttg
    20101 ccagatggtc ttgcctgatt cagatttgga cttggtagca gttttggatc cttttgagtc
    20161 tgagtcagaa tggcagggta ttcctgtttt caaggataag gctgatttag ctggttttga
    20221 agcggatgtc tgggtagact ttactactcc agctgttgcc tacgaaaata cacgttttgc
    20281 tcttgaaaat ggctttgctc cagtagttgg aacgactggt ttcacgagtg aagaaattgc
    20341 agagttaaaa gaattttctc gtgcccaaga cttgggtggc ctgattgccc ctaactttgc
    20401 cttgggtgct gtcttactca tgcaatttgc gacgcaggct gccaaatatt tcccaaatgt
    20461 ggagattatt gagctccatc atgacaagaa aaaggatgct ccgagtggaa cagccattaa
    20521 aacagctgag ttgatggcag aggttcgaga gtccattcag caaggcgcag cagatgagga
    20581 agagctgatt gctggtgctc gtggtgctga ctttgatggt atgcgcatcc actcagttcg
    20641 tttgccaggt ttggtagccc atcaggaagt catctttggc aatcaaggag aaggattgac
    20701 cctccgtcat gactcctatg atcgcatctc cttcatgaca ggagtcaatt tgggaattaa
    20761 agaagttgtc aagcgtcatg agcttgtcta tggattagaa cacttattat gagattaacg
    20821 caaatgcctt ctgaatttca gaaggcttta ccagtattag aaaaaattaa agaagcaggc
    20881 tttgaggctt attttgttgg gggctctgtt cgagatgccc ttctccatag ccctatccac
    20941 gatgtggata ttgcgacgtc ttcttatcca gaagagacca agcagatttt tccgcgaaca
    21001 gccgatatcg gaatcgagca tggaaccgtc ttggtcttag atggggatga ggagtatgag
    21061 gtaacaacct ttcggacaga ggatgtctat gtggactatc gcagacccag tgcggtttcc
    21121 tttgtgcgct cgctagaaga agacctcaaa cgccgtgatt tcacagtcaa cgcctttgcc
    21181 ttggatgaga caggagaaat cgttgacttg ttccatggtt tagaagattt ggaaaagcaa
    21241 gtcttgcgag cagttggagt ggctagtgag cgtttcaacg aagatgcttt acggattatg
    21301 cgtggtttcc gttttcaggc tagtcttggt tttgcacttg agccagaaac atttaaagct
    21361 atgaagacct tgacgccgct tttggagaac atttctgtag agcgtacctt cgtcgagttt
    21421 gataaactct tgctggctcc attttggaga aggggcttgg cttccatgat tgagagtcaa
    21481 gcttatgact atctccctga tatggcatct agccagaaca agctcaacag actgtttgat
    21541 ttggagactg attttacttt tgaatcctct gaacaagcct gggcggcttt actatgggct
    21601 ttggagattg aaaatgcgca gtcatttttg aaatcttgga agacctcacg ccagtttgcc
    21661 aagcaagttc aggatttgct gattattttg gctctgcgtg aaaatggaga attgagcaag
    21721 cgagattgtt atcgctttga catagatttg cttttacagg ctgaaaatct tcgtcaagct
    21781 cgaggaaaag aagtcaaccc acaagccatc acagaaaaat accaaagctt gactatccat
    21841 gacaagaaag agattcagat taatggcgat attttgatca aggaatatgg ctatcagcca
    21901 ggcccagact tgggagagat tttaacagag attgagtttg ccattgtcga tggagaattg
    21961 gagaataatc gtgaagccat ccatgcttac ctgagggaga aaaaatgagt gattttatcg
    22021 ttgaaaaact aagtaaatcc gttggtgaca agaccgtttt tagggatatt tcctttatta
    22081 tccatgactt agacagaatt ggtttaatcg gtgtcaatgg gactggcaag accacccttt
    22141 tggacgtcct ttctggtgtt tctggctttg atggggatgt cagtcctttt tcagctaaaa
    22201 atgattacca gattggttac ttgactcagg atcctgattt tgatgataga aagacagttt
    22261 tggatacggt tctatctagt gaactcaagg aaatccagct cattcgtgag tatgaattga
    22321 ttatgctcga ctatagtgag gacaagcagg cgcgtttgga acgtgtcatg gcagagatgg
    22381 actctctcca agcttgggaa atcgagagtc aggtcaagac cgttcttagc aaattgggca
    22441 ttcaagactt atctactcct gttggggaat tgtcaggtgg tctgagaaga cgggtacagt
    22501 tggcacaagt cttacttggc aaccacgacc tcttgctttt ggatgagccg accaaccatc
    22561 tggatattgc gattattgag tggctgaccc tctttttgaa aaattctaag aagaccgtcc
    22621 tttttatcac tcacgatcgt tatttcttag acgctttgtc aacacggatt ttcgagttgg
    22681 agcgagcagg cttgacagaa tatcagggca attaccagga ctatgttcgc cttaaggcag
    22741 aacaggacga gcgcgatgca actctacttc ataaaaaaga acaactctac aaacaagaac
    22801 tggcctggat gcgcagacaa ccgcaggcac gtgcgaccaa gcaacaagct cgtatcaatc
    22861 gtttccatga tctgaaaaag gaagtttcag gcagtagtgc tgagacagac ttgactatga
    22921 actttgaaac cagtcggatt gggaagaaag tcatcgagtt tcaggatgtt tcctttgcct
    22981 atgaaaataa gcccattttg caagatttta atctcttggt acaggtcaaa gatcgtatcg
    23041 gaatcgttgg ggacaatggt gttggaaaat caaccctact taacctgatt gcaggaagtc
    23101 ttgagccgat agcaggacaa gttgtgattg gggaaactgt tcgcatcgcc tatttctctc
    23161 aacaaattga gggtttggat gaaagcaagc gtgtgatcaa ttacctgcag gaagtggcag
    23221 aggaggtcaa gaccagtggt ggttctacga cttccatcgc tgagttgctg gagcaattcc
    23281 tcttcccacg ttcgacgcat gggaccttga ttgagaaatt atctggtgga gagaaaaaac
    23341 gtctttatct cctcaaactg ctcttggaga aaccaaatgt tcttctttta gacgagccaa
    23401 ccaatgactt ggatattgca actttgacag tcttagagaa tttcttgcaa ggttttgcag
    23461 gtcccgtttt aacagtcagt cacgaccgct atttcttgga taaggtagcg accaagattc
    23521 tcgcttttga ggatggcaag attcgtcctt tctttggtca ttacaccgac tatcttgatg
    23581 aaaaagcttt tgaaacagat atggccaatc aagtgcaaaa ggccgaaaag gaaaaagtgg
    23641 tcaaggttcg agaagacaag aaacgcatga cctaccaaga aaagcaggag tgggcaagta
    23701 ttgaaggtga tattgaaacc ttggaaaaac gtatcgctgc tattgaagag gaaatgcagg
    23761 ctaacggctc tgactttggt aagctggcta ctctccaaaa agaattggat gagaaaaatg
    23821 aagcactcct tgaaaaatac gaacgctatg agtatctcag tgaatttgat agttaagact
    23881 attcgaaaat caaagtgcaa actatgtcgg cttcacattg ccgactcaag tacagcctgt
    23941 gaccggcttc ctaatttgca ctttgatttt tattgagtat aaaagaatag aaaaatcccg
    24001 tctcatgaaa ggatttctct attctttttt ggtttcttga tgaaagatat tttgccaggt
    24061 ttcgtggcga cgccagatac tggtatggat gataccatct aactcatagc agatgagttt
    24121 ggttttttga ctgatggaag taatttgaat gtccttgata gcagaattta actctttttt
    24181 ggtcttataa gcctctttat ccatctgctc tccatcttgg cgaatataga caaaatcatc
    24241 agtcaagagt tcttctagtt ggtttccttg gtcaatcaat tgttcacgca taagcaattt
    24301 tttatagaca ttgtctaaaa tttcatcctc agggataggt gctggttcga tatggacatc
    24361 ggtatcaaag acgccaaaac gctcctccag catagactcg acctgatccg cgatttcatg
    24421 gctttcaaaa acagacaagt caggattcat ctctagtgta atatccaggt agatgttgct
    24481 accgtaggtg cgacctcttt gcgatttgac cttgctgatt ttgggaattt ccatgatagc
    24541 cttttggtag tcctcgagca ggcggtcgtc aaagccatct gaaagactaa aggaagactc
    24601 gatgaagata tcataggcag tcttcaagat aaagaaagtg atgatgatag caaccagttt
    24661 atccacaatc ggataattga aactactagc taggatggca atggcggttc caagtgaggt
    24721 aacagcgtca gaaagattgt ccttagcagc tgccttcagc gccttggagt tggatttctt
    24781 actgaggcga gtattgtaga gatagaccac aaacataatc gctgcagaaa tgattcctag
    24841 agttgcacca agaggatcaa tgaccgtttc ttcccgactg agaatctttt gaatggtatc
    24901 tcttagaata tcgaaaccga catagaacat gatgatagaa gtgatcaagc ttgccaaatc
    24961 ttcaatcttc caatgaccaa aacggtggtc acggtctgca ggctggcgcg ccatccgaat
    25021 cccgattaag agggccacat ttccaatgat gtccgatacg ttattaaaac catcggccac
    25081 caaactggat gaatgaagga gatgaccagc tgctaattta gctgcagaca agatcaaata
    25141 ggtcgaaata ctgataatgg ctccacgctc agctaacttg agatttgaga tagattgctt
    25201 cattcaactt cctttctagt cacatagcat tctaccatta tactggtttt caaggaaaaa
    25261 ttcaaataag gcagttttta ctctttgaaa actccccaaa atttggtata atggtactac
    25321 tcaagggagt agctggcaga aacctgtgat agtgtcgtca ttccgaattt tatgctgaaa
    25381 agtatgcttt ccggccctat cttaaacagc gagacttgtt atgattaaca ggtctctttt
    25441 tgtttgtcgt gaaaagatat gacagaaaga gagtctgttt tgcacacaat gtcaaggagg
    25501 agacacatgt caaaagaaca aaaacgccaa gcgttttata ctcagagccc tgaagaggtc
    25561 ttgcaggctg tggatgcgac cgagcaaggt ttgtcatcaa gtgaggcgga aaagcgcctt
    25621 gccgaatttg ggcacaatga actcgaagaa ggcgagaaac gatcaatctt ggtcaaattc
    25681 atcgagcaat ttaaggattt gatgattatc atcctagttg cggcagcaat cttgtcagtc
    25741 gtgacttctg gtggggaaga tatcgcagat gccattatca tcctagctgt ggttatcatc
    25801 aacgctgcct ttggtgttta ccaagaagga aaagctgaag aagctatcga agccctcaaa
    25861 tccatgtcta gtccagttgc ccgcgttctt cgtgatggac acatggcaga gattgactct
    25921 aaagaattgg taccaggcga tatcgttgcc cttgaagcag gtgacgtggt accagcggac
    25981 ctacgtttgc tagaagccaa ctctcttaaa attgaagaag cagccttgac gggtgaatct
    26041 gtaccagtcg aaaaagactt gtcagtcgag cttgcgacag atgctggtat tggtgaccgt
    26101 gtcaacatgg ccttccaaaa ctcaaacgta acctatggtc gtgggatggg tgttgttgtc
    26161 aatacaggta tgtacactga agttggtcat attgctggta tgcttcaaga tgcggatgag
    26221 actgatacac cactcaaaca aaacttgaac aacctttcta aggtcttgac ctatgctatc
    26281 ttggtcattg cccttattac ttttgtagtg ggtgtcttca ttcaagggaa aaatccactt
    26341 ggtgagttgt tgacttctgt tgcccttgcc gttgcagcca ttccagaagg acttcctgct
    26401 atcgttacca tcgttctttc tcttggtact caagttttgg ccaaacgaca ttccatcgtt
    26461 cgtaagttgc cagcagttga aacacttggt tcaactgaaa tcatcgcttc tgataagact
    26521 ggtacgctga ctatgaacaa gatgacagtc gaaaaagtct tctacgatgc ggttctacat
    26581 gactcagctg atgatattga actaggtctt gaaatgccac tacttcgttc agttgtcttg
    26641 gccaatgata cgaaaatcga tgtggaaggt aacttgattg gtgacccaac cgaaacagcc
    26701 tttatccaat atgccttgga caagggctat gatgtcaaag gtttcttaga gaaatatcct
    26761 cgtgtagctg aattgccatt tgactctgac cgtaagctca tgtcaacagt tcacccattg
    26821 ccagatggtc gtttccttgt agcagtcaag ggtgcgccag accaactctt aaaacgttgt
    26881 cttcttcgtg ataaggctgg ggatattgct ccgattgatg agaaggttac aaatctcatt
    26941 catacaaaca attctgaaat ggctcatcaa gccttgcgtg tccttgcagg tgcttataag
    27001 attatcgata gtattccaga aaatctcact tctgaagagc ttgaaaatga tttaattttt
    27061 actggtttga ttgggatgat tgaccctgaa cgtcctgaag ccgctgaggc tgttcgtgtg
    27121 gctaaggaag cgggaatccg tccgattatg attacaggtg accaccaaga cacagcggaa
    27181 gccattgcca aacgtttggg aatcattgac gcaaacgata cagaaggtca cgttttaact
    27241 ggtgctgaac tcaatgaact gtcagatgaa gaatttgaaa aagtcgttgg tcaatactct
    27301 gtttatgccc gtgtgtctcc agaacacaag gttcgtatcg tcaaggcttg gcaaaaacaa
    27361 ggtaaagtcg ttgccatgac aggtgacggt gtcaatgacg cgccagctct gaaaacagcc
    27421 gatatcggta tcggtatggg aatcactggt acagaggttt ctaagggggc ttctgatatg
    27481 attcttgcag atgataactt tgcgactatt atcgtcgcag tggaagaagg acgtaaggtc
    27541 ttctcaaaca ttcaaaagac tattcagtac ctactttctg ctaatactgc tgaagtatta
    27601 accatcttcc tatcaacctt gtttggttgg gatgtcttac agccggttca tcttttgtgg
    27661 atcaacttgg taacggatac cttcccagct atcgctcttg gtgttgaacc tgcggaacct
    27721 ggtgtcatga atcataaacc acgtggacgc aaggcaagct tcttctcagg tggtgttttg
    27781 agttctatca tttatcaagg tgtactccaa gcagctcttg ttatgagtgt ttatggcctt
    27841 gcgattgctt acccagttca tgtgggtgac aatcatgcta ttcatgcaga tgccctaacg
    27901 atggcctttg caacccttgg tttgattcag ctcttccatg cctacaatgt caagtctgtc
    27961 taccaatcca tcttgacagt tggcccattc aagtctaaga cctttaactg gtccatcttg
    28021 gtatccttta tccttctcat ggcaacaatc gttgtagaac cgcttgaagg aatcttccac
    28081 gtaaccaaac tagacttgtc acaatgggga attgttatgg ctggaagctt ctcaatgatt
    28141 atcatcgtcg aaatcgttaa gtttattcaa cgcaaactcg gttttgacaa gaatgcgatt
    28201 taagggaggt ttcggatgtc agcatatcaa ttaccgaccg tatggcagga tgaagctagt
    28261 aatcaaggag cttttacggg gctaaacaga ccaacagcag gtgcccgttt cgaacaaaac
    28321 ttgccaaaag gagaacaagc ttttcagctt tattcactgg gaacaccaaa tggtgtgaag
    28381 gttactatct tattggaaga attactagaa gctggtttta aggaagcggc ttacgacttg
    28441 tataagattg ctatcatgga tggggatcaa ttcggctcag actttgtgaa gctcaatcca
    28501 aattccaaga ttccagcctt attggaccag tcaggtactg aaaacgtaag agtctttgag
    28561 tctgctcata ttcttcttta ccttgctgag aaatttggag cctttttacc aagtaatcct
    28621 gtggaaaagg tagaagtttt gaattggcta ttctggcaag caggtgcagc accttttcta
    28681 ggtgggggat ttggacattt cttcaattat gctcctgaaa aattggaata tcctattaac
    28741 cgttttacga tggaagtgaa acgccagttg gatttattgg ataaggaatt ggctcagaaa
    28801 ccttatattg caggcaatga ctatacgatt gcagatattg ctatctggtc ttggtatgga
    28861 cagttagttc aaggaaatct ttaccaaggt tctgcaaaat tcttggatgc ctcaagttat
    28921 caaaatctag taaaatgggc agaaaaaatt gccaatcgtc cagctgttaa gcgtggcttg
    28981 gaagtaactt atacagaaat taaatagcaa aaaagtcatc ttcggatggc tttttgctac
    29041 agttgaggga aagggtttgc agttatcggc ttttgtgcta taattgctat aatatagtaa
    29101 aaagcaagag gagtgtgagg aagtggaaaa gaaaattgtg aaggatatct tatttttatc
    29161 tcaagtgtct cagccggcaa gtcaggagga cctttatctt gccagagatt tgcaggatac
    29221 actcttagca aatcgtgata cctgtgttgg tctagctgcc aatatgattg gggtgcagaa
    29281 gcgcgtgatt atctttaatc ttggcttggt tcccgtggtc atgtttaacc cagtgcttct
    29341 gtcctttgaa ggatcctatg aggcagaaga aggctgtttg tccttggtag gtgtgagatc
    29401 aactaagcgt tatgaaacca caaggcttgc ctatcgtgac agcaagtggc aggaacagac
    29461 cattaccttg acaggcttcc cagctcagat ttgccagcat gagctggatc acttggaagg
    29521 acgaatcatt taggaggaaa gcaaatgaaa cgaatagtct ttgaacttat ttttatcgca
    29581 acgacctggt atatcttttt accgcccctt aacctgacca gctgggaatt tctcttcttc
    29641 ctctgtgggc atttgttagt tgtggcaatc ttatttggct ttggcaaggg gataaacctt
    29701 gtcaaaacgg ttcatgtgcg ccacggtaag gcggaagctg ccttaaatct tgagggtttc
    29761 aaaatcaatc ggttagggaa aattctgtta gcttcgattg gaggaattct tctcttggca
    29821 gctttggttt ccttggtaac ttccagcatg tttcaggcta aaaattatgc caatgtagtc
    29881 acggttacgg aaaaagactt tactgaattt cctaggagtg acaccagtaa ggttcctatc
    29941 ctagatagaa gtactgctga aaaaattgga gaccgctact tgggttccct aaccgataag
    30001 gtgtcgcaat acgtagcggc agatacctat acccaattga caattgatgg gaaaccttat
    30061 cgggtcacac cactagaata tgcagaccct atcaaatggt ttaacaatca agccaaggga
    30121 atcggtgagt atatcaaggt ggacatggta actggaaatg cggatttggt ggacttgaag
    30181 acaccaatca agtattcaga ctcggagtat tttaaccgtg atgtcaaacg tcacctgcgc
    30241 ttgaagtacc cgactaaaat ctttaaaact ccatcttttg aggtggacga tgagggcaat
    30301 cctttctatg tagcaacggt ttaccaaaag caatttggac ttgcggttcc tcgtcctgct
    30361 tcagtcatta tcttggatgc tacaaatgga gaaaccaagg aatacagctt atcagatgtt
    30421 ccagaatggg tggacaggat ctatccagca gaggaaacca ttgagcaaat caactacaac
    30481 ggcaagtaca aggacggttt cttgaatgcc atgatttcca agaaaaacgt gacccagact
    30541 accaatggct ataattactt gtctatcggt aatgacatct atctctacac aggtgtgacg
    30601 tcggctaatg cggatgagag taatcttggt ttcatccttg aaaatatgcg aacaggagaa
    30661 attactaagt atagcttggc ttctgcgaca gaagaatcag cccgtgaatc agcagaaggt
    30721 gctgttcagg agaaatccta caaagcaacc ttcccaatcc tcatcaacct caatgacaag
    30781 cctctctaca tcatgggctt aaaggacaat gctggcttgg tcaaagagta cgccctggta
    30841 gacgcagtcg agtaccaaaa tgttatcgtt gctactacag tggaagagat gctcagcaag
    30901 tatgccaata aaaacgacct tgaaattgac aatgcaacga cagaaagcat caatggagta
    30961 gtagcagacc tcaaatcagc tgttatcaag ggagacactg tctacttctt taaagttgat
    31021 ggcaagatat acaaggtcaa ggcttcagta tccgatgacc ttccttacct tgaaaatggt
    31081 aaaaccttcg aaggtcaagt aggaaaagac aattatctca agacctttaa gctacggtaa
    31141 aaataggttt ttttcagaaa gtatatgtta taataaggta aattaagcct aatggaggac
    31201 aaagaaatgg gtttttattt gatggttgct tcaatgctac tcggactgct cgctttgaag
    31261 ataggttttt ctcagttcaa ggagaaaaaa gataaattct tatctatctt aacaagttta
    31321 gctggcacag cccttgttct cgtggctgtc tggttaggat ggcccaaata aatagaaaaa
    31381 cctcggtcaa accgaggttt ttcaaatgaa tttcttggtt gtggctaaaa aatatgctac
    31441 actatcaata tgaaaatttt aatcccaaca gcaaaagaaa tgaacacaga cttcccaagt
    31501 atcgaggcaa ttcctttaaa accagaaagt caggccgtgc ttgatgcctt ggctctctat
    31561 tctgccagtc aattggagag tttctacaag gtatcagctg agaaagcggc ggaagaattt
    31621 caaaatatcc aagctttgaa aaggcaaact gctcaacact atccagcctt gaaacttttt
    31681 gatgggctta tgtaccgcaa cattaagaga gataagctga ccgaggcgga acaagattat
    31741 cttgaaaatc atgttttcat tacctcggct ttgtacggtg ttgttccagt cttgtcaccc
    31801 atggctcctc accgtttgga ttttttgatg aaattaaaag tcgctggtaa gactttgaag
    31861 agccattgga aggcagccta tgatgaaact ctgaagaagg aagaagtgat tttctctctc
    31921 ttgtcatcag agtttgagac tgtattttct aaggaaatca gagcaaagat ggtgaccttc
    31981 aaattcatgg aggatagagg cggtcagctg aagattcact caactatctc caagaaagcg
    32041 cgcggggcct ttctaacagc tttaatagaa aatcaagtac aaactgtggg ggaagcacgt
    32101 cgcttgaact ttgctggatt tgtttaccga gaagatttgt cacaaccaca gggattggtt
    32161 tttgtaaagg aagtatagaa atcagatatt ttgtatttgg caatatgtat catcagctat
    32221 tttagtttat aaatgagaaa aaagcgaaca agctagtctt ctgagtgtca gaaaagtagt
    32281 cttgttcgct tttattatgt tatactcttt gaaaatctct tcaagccacg tcagtgtcgc
    32341 cttaccgtat atatgttact gacttcgtca gttctatcta caacctcaaa gcagtgcttt
    32401 gagtagcttg cggctagctt cctagtttgc tttttgattt ttattgagtg ttagttactt
    32461 gatagcttcg accaagtctt gagcttgttt ttcaagtgag tttaggactg tttcttcaag
    32521 aaccaatttt ccgtctgccc aggcagagtc attaacacgt gcagcagtga aatcaccaac
    32581 gccttgtgta cggataaatg gcaagaggtc tttgtagata gcgaaaagtt gatcgtgccc
    32641 tgcattggct acagatgata ctgtgacaaa cttgtcttga agggcagaaa cgccacgtgt
    32701 atcagacaag tcaagggcac gagatagcca gtcaagcaag tttttcactg taccagggat
    32761 agagaagttg tagactggag agaaaatcca gatagcatcc gcaacgagaa ctgcttcacg
    32821 agcagcagct acagctggat gtgttggaac ttccaaatct tggctgaaga gaggaagggc
    32881 tgaataatca aggtagctaa cttccgcttt accagcaagt gctttctcag cttcgagcgc
    32941 catttggtgg ttgaaagaac cttgacgtag tgatccgacg ataaataata cttttttaga
    33001 catgatgtgt ctcctttttc ttcaaatttt gagaactctc tcgttgttat aaaatacatt
    33061 acaacaaaac aaaagaactt gcaagaaatt tgctcagagt ttatttgaaa attgtaaaat
    33121 ttcggaaact ctagtcattt gtgactgaaa aagtaagaac tgtttcactt tcaaaatcgg
    33181 ttttgtttga agggagaaat agaattggtt gctcaaagca cagctttgag gttgcagata
    33241 aagctgacgt ggtttgaaga gattttcgaa gagtattctt atattcactt gtaataaagc
    33301 ttaatagatg gtaaaataga cgagtgaaaa aaagacaaaa caaagcaaaa aataatctac
    33361 tgtggcagta tggtctaggg atgacgattt tgtttgtggt tatcagttct tcctttctgt
    33421 atatggtttc tcttagcatg aaaccctatc aaacagctaa aagtgaagga gaaaaattag
    33481 ctcagcagta tgcaggatta gagcaggccg atcaggttga tttatacaat ggcttggaat
    33541 cttattacag cgttcttggt cgtaataaac agcaagaagc acttgctgtt ctgattggaa
    33601 aagatgatca taagatttac gtttatcagc taaatcaggg tgtttcacaa gaaaaagcag
    33661 aaacggtttc taaggaaaag ggagctggcg aaattgacaa gattatcttt ggtcgttatc
    33721 aagataagcc aatctgggaa gtcaagtcag gatctgattt ttatctagta gattttgaaa
    33781 caggagcatt ggtcaacaag gagggcctat gaaattatcc aaccgtgttt tagaaatgga
    33841 agaaagtgtg actttggctg ctggagcccg tgccaaagca ctgaaggctg agggtagaga
    33901 tattttgtct ctaaccttgg gtgagccaga tttcactact cccaaaaata tccaagatgc
    33961 cgccattgca tcgattcgag atgggcgtgc ttctttttat acagtaacct caggtctgcc
    34021 agagcttaag gcggcggtca atagctactt tgagcgcttt tacggctatt ctgtagcgtc
    34081 aaatcaagtg acagtcgctg cgggagccaa atattctctc tataccttct ttatggctgt
    34141 ggtcaatcca ggtgatgaag tcatcatccc aaccccatac tgggttagct atggagatca
    34201 ggtcaagatg gcagaaggcg ttcccgtctt tgtttctgct aaggaagaca atcactttaa
    34261 ggtgaccgta gagcagttag aagcagctcg cactgacaag accaaggttt tggtgctgaa
    34321 ttcgccatct aatcccacag gtatgattta cacccgtgag gaactcttgg caattggaaa
    34381 ctgggctgta gaaaatgata ttctcatcct agcagacgat atctatggcc gcttggttta
    34441 taatggtcat gagttcacac cgatttctag cctatcggaa gcgattcgca agcaaacagt
    34501 ggtcatcaat ggtgtgtcta aaacctatgc catgactggt tggcggattg gttatgccgt
    34561 tggagaagca gacattatcg ctgccatgtc caagattgca ggtcaaacaa cttcgaatcc
    34621 gtcagcagta gcccaatatg cagcagttga ggctctatca ggtgagcaag atactgtaga
    34681 aagcatgcgt caggcctttg aggaacgtct taataccatc tatcccctcc ttgcagaggt
    34741 gccaggattt gaagtggtca aaccgcaagg ggccttctat ctcttcccaa atgtcaaaaa
    34801 ggccatggag atgaaaggct acacggatgt gacagacttt acaactgcta tcttagaaga
    34861 agccgaagtg gccttggtaa caggagctgg atttggagca ccagaaaatg tccgcctcag
    34921 ctatgcgaca gacctagaca cgcttaaaga agcagtcgaa cgcttgaaag catttatggg
    34981 tagtgagaat gattgatcat tttgagatta aggtaaagga tttacaaatt tcagaaggat
    35041 tttataggag ttttctcgct cctttggact ataaattggc ttttaagact agttctctaa
    35101 ttagttttct atccccgaac agccctcatc ctggtggtga tttttggctg actcagggaa
    35161 cacaagatcc tgttcatttt gcttttttag cagaaaataa ggaagaagtt taggcttgtt
    35221 atgaggctgg cttagaggca ggtgggcgag ataatggggc tcctggttat cgaagtgagc
    35281 gtccgattta ctatgctgct tttatgattg acctggatgg gaacaatata gaggtggttt
    35341 gccataaaga ataaaaaaga aaaggaaaaa taatgacaaa acgtgtaacg attattgacg
    35401 taaaagacta tgttggtcag gaagtgacga ttggcgcttg ggttgccaat aaatcaggaa
    35461 aagggaaaat cgctttctta caattgcgtg atggaacagc cttctttcaa ggtgtggctt
    35521 ttaaaccaaa ctttgtcgaa aaatttggtg aagaagtggg acttgagaag tttgatgtta
    35581 tcaaacgctt gagccaagaa acgtctgttt atgtgacagg tattgtcaaa gaggacgaac
    35641 gttctaaatt tggctatgag ttggacatca cagacatcga agtgatcggt gaatctcaag
    35701 actacccaat cacaccaaaa gaacacggaa cagacttttt gatggataac cgtcacttgt
    35761 ggctacgctc tcgtaagcaa gtagctgtgt tgcaaatccg taacgctatt atctatgcaa
    35821 cttatgagtt ctttgacaag aacggtttta tgaagtttga cagcccaatt ctttcaggaa
    35881 atgcggcaga agattctaca gaactctttg aaactgacta cttcggaacg ccagcctact
    35941 tgagccaatc aggtcagctt tacctagaag caggggctat ggctcttggt cgtgtctttg
    36001 actttggtcc agttttccgt gctgaaaaat caaaaacacg ccgtcacttg actgagttct
    36061 ggatgatgga tgctgagtac tcatacttga cacatgatga gtcgcttgac ttgcaagaag
    36121 cttatgtgaa agctcttcta caaggtgttc ttgaccgcgc gcctcaagcc ttggaaacct
    36181 tggaacgtga tacagaactc ttgaaacgct acattgcaga gccattcaaa cgtatcactt
    36241 acgatcaagc cattgacctc ttgcaagagc atgaaaatga tgaagatgct gactacgagc
    36301 atcttgagca tggtgatgac tttgggtcac cacacgaaac ttggatttca aaccactttg
    36361 gtgtgccaac atttgtcatg aactatccag tagccatcaa ggccttctac atgaaaccag
    36421 ttcctggaaa tccagagcgc gtgctttgtg cagacttgct tgctccagaa ggctatggag
    36481 aaattatcgg tgggtctatg cgtgaggaag attacgatgc ccttgtcgct aagatggatg
    36541 aacttggcat ggatcgtaca gaatatgaat tctaccttga ccttcgtaaa tacggtacag
    36601 ttccacacgg aggatttggt atcggtatcg aacgtatggt aacctttgca gcaggaacaa
    36661 aacatatccg tgaagctatt ccattcccac gtatgttgca ccgtatcaaa ccataagaac
    36721 aggaaaacgc ccatgtggcg ttttttcgaa ttctagtgtt tttacttgcg taaaaaaatt
    36781 ttttctagta taatagttta ttgtgagcga acctcactta ccccttgcaa agtcttgggg
    36841 tcattagacc aaaaggagga acatatcaat ggctaaatac gaaattcttt atatcattcg
    36901 tccaaacatt gaagaagaag ctaaaaacgc tttggtagca cgttttgact ctattttgac
    36961 tgacaacggt gcaactgttg ttgaatcaaa aacttgggaa aaacgtcgtc ttgcatacga
    37021 aatccaagat ttccgtgaag gactttacca catcgttaac gttgaagcaa atgacgatgc
    37081 agctcttaaa gagtttgacc gtctttcaaa aatcaacgct gacattcttc gtcacatgat
    37141 cgtcaaaatt gacgcgtaag aaggtaaact atgattaaca atgttgtact tgtagggcgt
    37201 atgacacgtg acgctgagtt gcgttatacc ccatcaaatg tagcagttgc gacttttact
    37261 cttgcagtaa accgtacatt taagagtcaa aatggtgaac gtgaggctga ttttatcaat
    37321 gtcgttatgt ggcgccaaca ggctgaaaat cttgctaact gggctaaaaa aggctcactt
    37381 atcggggtga caggtcgtat ccagactcgt agttacgata accagcaagg acaacgtgtc
    37441 tacgtgacag aggtcgtggc tgagaatttc caaatgttgg aaagccgtag tgtgcgtgag
    37501 ggtcacacag gtggagctta ctctgcacca actgcaaact attcagcacc tacaaattca
    37561 gtactagact tttcacgtaa tgaaaatcca tttggagcaa caaacccatt ggatatttca
    37621 gatgatgatt taccattcta atggacaact aaacttataa aggagaaaaa acatggctca
    37681 acaacgtcgt ggcggattca aacgccgtaa aaaagttgat tacatcgcag caaacaaaat
    37741 tgaatatgtt gattacaaag atactgagct tcttagccgt ttcgtttcag aacgtgggaa
    37801 aatccttcct cgtcgtgtaa caggaacttc agctaaaaac caacgtaaag taacaacagc
    37861 tatcaaacgc gctcgcgtaa tggctttgat gcctttcgta aacgaagatt aaagaaaaga
    37921 cccggttggg tctctttttc atgagcttga aaacaagaaa gcgaattaag accccaatgg
    37981 gtcttttttc acaaactttg aaatgagaga gcgagttagg ctcagtggct ccctttttac
    38041 gagtttgaaa acaagagaac acattaaaag cacgatggag ctttttcata aatatgtctt
    38101 ttttctcatc caagaaatta aaataaaaat gataaacttt ttgaaaatag gggatttgcc
    38161 tactttatga tataatggaa agagttagat tgaaagaggt agtgagatgg acgcgaaatt
    38221 aagatacaag gcaaagaaga tcaagattgt cttttttgat attgatgata cattgcggaa
    38281 ttcaaagaca gggtttattc caactacaat tcccactgtt tttaaacagt tgcgtgaaaa
    38341 aggaatttta acaggaatcg cctctggacg tggcattttt ggtgttgttc cagagattcg
    38401 tgatctcaag cctgactttt ttgtaacttt gaatggggct tatatcgaag ataaaaaagg
    38461 tcaggtcatt tatcagcatc agattgaaaa gtcagatgtt gaggagtata tctcttgggc
    38521 taagcaagaa ggaattgagt atggcttggt tgggagtcat gatgccaagt tgtcgactcg
    38581 caccgatatg atgagtgaag ctatcaatcc aatttatacc gacttagatg tagatcccga
    38641 tttccatgaa aaagaagata tctatcagat gtggactttt gaagataagg gagatgactt
    38701 gcacttgcct gacagtctct cagacaaact tcgcatggtt cgttggcatc aacattcgtc
    38761 tgatattgtg ccgatttcag gctccaaagc gacgggggtg gaaaaagttg tggaacacct
    38821 tggcttgaaa ccagagaagg tcatggtttt tggagatggt ctcaacgact tggaactctt
    38881 tgattatgct ggaatcagcg ttgccatggg aatttctcat gataaaatca aagaaaaagc
    38941 agattatatt acaaaaacat tagaagaaga tggcattttt gatgccttag aagtatttgg
    39001 tatggtagaa aaagaattgc attttccaca agtagacatt gaaacagtag aaggtcctct
    39061 tgcgactatt aagaccaatc acggagactt acgtatcaag cttttccctg aacatgctcc
    39121 taaaacagtg gctaactttg tatctctttc aaaagatggc tactatgatg gtgtcatttt
    39181 ccaccgtatt atcaaggact ttatgatcca aggtggaaac ccaactggaa ctggtatggg
    39241 tggcgagtca atctacggcg aatcttttga ggatgaattc tcagaagagc tttacaatat
    39301 ccgtggtgct ctttccatgg caaatgctgg tccaaatacc aacggcagcc agttctttat
    39361 cgtgcaaaac caacacctac cttattctaa gaaagaaatt actcgtggtg gttggccaga
    39421 accgattgca gaaatctatg ccaatcaagg tgggacacct cacctagacc gtcgtcacac
    39481 ggtttttggt cagttagctg atgaagcatc ttacgctgtc ttggatgcca ttgctgctgt
    39541 tgagacagga gctatggaca agccagttga agatgttgta attgaaacta ttgaaatcga
    39601 ggactaagat gaaaatcggt gataagctaa aggggcgtat tacagggatt cagccctacg
    39661 gtgcctttgt tgagttagag acgggtgata cggggctgat tcacatttca gaaattcgga
    39721 caggatttat tgaaaatatt catgaaacct tgaaagtcga tgaagaggtt caagttcagg
    39781 tagtggattt agatgaattt acggggaaag ccagtctttc tatccgcact ttggaggaag
    39841 aaaagtacca gtttcctaga cggagacgct tttcaagcga ccgctttaac tacggttttg
    39901 ctccctttcg acgaatgcta ccaatctgga ctggagaagc cctgcaccat ttaaaaaaga
    39961 agaagtagtt tagagtttta tattttttgt aatgttaata taaatttgct ataataggac
    40021 catggaagaa gaacaattat taaaatcagg agagcgcatt aaccagctct tttcgacaga
    40081 tatcaaaatc attcaaaata gagaggtttt tagctattcg gtggatagtg ttctcttatc
    40141 acgatttcca cgttttccta agaaggggtt gattgtggat ttctgtgctg ggaatggagc
    40201 agtggggctt tttgctagca ctcgtactca agcacagata ttgtctgttg agattcagga
    40261 gcgtttggcg gatatggctg aacgctctgt ccgtttgaat ggattagagg agcaaatgca
    40321 ggtcatctgc gatgatttga aaaatatgcc tgctcacatt caggggagta aggtggatat
    40381 gattttgtgt aatccaccct atttcaaggt ggatccttat tctaatctga acgagagtga
    40441 acattatctc ttggctcgac acgaaatcac gactaatttg gaagaaatct gtcatagtgc
    40501 ccagagtatt ctcaagtcta atgggcgttt ggccatggtt catcgtcctg atcgacttct
    40561 ggatattttg gatacgttaa aacgacataa tctagcccct aagcgcctgc agtttgttta
    40621 tccaaaaaga gaaaaggaag ccaatatgct tttgattgag gcgatcaagg atggctcaac
    40681 aagtggcttt aaggtcttac ctcctttgat tgtccacaat gatgatggct cttatacgcc
    40741 cgaaatcgaa gagatttatt atggatcata aggcttatat gtacgttctg gagtgtcgtg
    40801 atggatccta ctatataggc tatacgactg atatgagaag acgcctcgct atccacaata
    40861 gtgggaaggg agccaaatat acacgagcac gcttgccagt caaacttatc tatgctcaag
    40921 gttttgccag taaggaagaa gccatgtcgg ctgaagctct tctcaagcgt aagaagaggc
    40981 cacagaagga agaattttta tctgaaaatc aagatagaaa tttactcagt tattttgaag
    41041 aatcgtgggg agtcctttga ctcctcttac ctttgtcaaa aagcgaaaaa aatgctacaa
    41101 taaagaaaat aaacaaaatg aggtattatc atgtccaaga ttctagtatt tggtcaccaa
    41161 aatccagact cagatgccat cggatcatct gtagcttttg cctaccttgc aaaagaagct
    41221 tacggtttgg atacggaagc tgttgccctt ggaactccaa atgaagaaac agcctttgtc
    41281 ttgaactatt ttggtgtgga agcaccacgt gttatcactt ctgccaaagc agagggggca
    41341 gagcaagtta tcttgactga ccacaatgaa ttccaacaat ctgtatcaga tatcgctgaa
    41401 gtagaagttt acggtgttgt agaccaccac cgtgtggcta actttgaaac tgcaagccca
    41461 ctttacatgc gtttggagcc agttggatca gcgtcttcaa tcgtttaccg tatgttcaaa
    41521 gaacatggtg tagctgtgcc taaagagatt gcaggtttga tgctttcagg tttgatttca
    41581 gatacccttc ttttgaaatc accaacaaca cacccaacag ataaaatcat tgctcctgaa
    41641 ttggctgaat tggctggtgt gaacttggaa gaatatggtt tggcaatgtt gaaagctggt
    41701 accaacttgg ctagcaaatc tgctgaagaa ttgattgaca tcgatgctaa gacttttgaa
    41761 ctcaacggaa ataatgtccg tgttgcccaa gtgaacacag ttgacatcgc tgaagttttg
    41821 gaacgccaag cagaaattga agctgcaatg caagctgcca acgaatcaaa cggctactct
    41881 gactttgtct tgatgattac agatatcgtc aactcaaact cagaaatatt ggctcttggt
    41941 gccaatatgg acaaggtcga agcggctttc aatttcaaac ttgaaaacaa tcatgccttc
    42001 cttgctggtg ccgtttcacg taagaaacaa gtggtacctc aattaactga aagctttaat
    42061 gcgtaagatt ttgggtgtca gctcaaaatc ggaaagtcta gtttgcctta tatcgaaagg
    42121 agtttcggct cctttttttc taggagtgaa gtatgttaga aaatggcgat ttgatttttg
    42181 tgagagatgg gtcagacatg ggacaggcca tccagacttc cacaggtaac tatagccatg
    42241 ttgccattta tttggatggg atgatttatc atgctagtgg acaggctggt gttgtctgtc
    42301 aagaaccggc agacttcttt gagtccaatc atttgtacga cctctatgtt tacccagaaa
    42361 tggatatcca gtcggtgaag gaaagagctt gcagacatct tggagcaccc tacaatgctt
    42421 ctttctatcc agatgcagct ggtttctact gctcccagta tatagcagaa atcctaccta
    42481 tttttgaaac tattcctatg aaatttggag atgaggaaca ggagattagt gatttttgga
    42541 gggagtatta catagaacta ggtctgcctg ttcctctgaa ccaagctggg accaatccta
    42601 gtcagttggc agcatcgcct ctgttacaat gtaaagaaag gaatcttcat gattcagatt
    42661 tttaatccat ctcgtttgac gagacagcca tttttggaga attgatccgc tatctggacc
    42721 agtatgagga tgtgattcta cgggaaatta aggctcaatt tccagatgtt gcagttgata
    42781 aactcatgga agagtatata aaggcaagct tgattctacg tgaaaataag cgctattacc
    42841 ttaattttcc tacgcttgaa tcacttgata gtcttgaact ggatcaagag atttttgtca
    42901 gagaagctag tccggtctat caagccttgt tggagcagag ttttgagacg gaattgcgca
    42961 atcaaatcaa tgcagctatt ttagttgaaa agacggactt tgcgcgcatt aaaatgaccc
    43021 tgtccaatta tttttacaag gtcaaacagc agtatccttt gacagaaaaa cagcaggagc
    43081 tctatgacat tttaggagat gttaatcctg agtatgccct caagtatatg acggcttttt
    43141 tgttgaaatt tctcaaaaaa gaccagctta tgcagaaatg ccgtgatatc tttgtggaca
    43201 gtttggttgt cttaggctat attgtgcaaa atgaagatgg aaagtatgag ttggctatcg
    43261 attttgataa ggagagatta actttctact tagcgtgatt tcttgtttct gagtacattg
    43321 tttgactttc cttagtattc ggtataaact atatgtaacc ggtaacacat atcggaataa
    43381 actaaaggag acaatcatat gtcacttgaa aacaaattgg aacaagcaac aggcgctgtc
    43441 aaagaaggtt ttggtaaagt tactggagac agcaagacag aacttgaagg agctgttgaa
    43501 aaaacagttg ctaaggcaaa agacgttgta gaagacgcaa aaggtgctgt agaaggtttg
    43561 aaaaacgttt ttactaaata ataggaaaaa atcaagggtt tcattttccc ttgatttttt
    43621 ctattcttat aaataatttt ctgcgacggc tgtatctcct gggtaggatt ctttcttgcc
    43681 ctggatgatt tggtaacaat cgactccctt acccgcaata ataactgcat ctaattcgtg
    43741 atttgtgata gccattgccg ccttgatggc ttcttggcga tccgcaatct tttcaacagg
    43801 atgattgatg tagctactaa tttcatctgc aatggccatt gggtcttcat agttagggtc
    43861 atcagcagtc agaaagactt gaatctcagg gtgttgattg aggaggaggc caaagtcctt
    43921 acgacgactt tctcccttgt ttcctgttga tcccagaacc agagcaatct ttccggtttg
    43981 atgagtttcc accacattga tgagtttttt cagactatcc ccattgtggg catagtcgat
    44041 gaagaccttg gctccatttt tctgagtgag gacttccata cgaccaggaa cgcgggttgc
    44101 agcgatgcct tttttgatgt cctcaagact tgctccgaga cggagacaag caagtccagc
    44161 agcaactgca ttttcttggt tgaagttgcc aatgagttgg atatcataat ctccagcgag
    44221 tttacccgta gctgaaaagc taaaggcttt ggaattctcg atttggttat caaattggct
    44281 accatagaaa tcatggtctt gatcttcaac ctgttctttc aagactgaga agtggtccat
    44341 gtcactgtta atgatgactg ctcggctatt ttccatcaag agacgcttgt ggtagaaata
    44401 gtcttcaaag ctagggtgtt caatcgggcc gatatggtct gggctgatat tgaggaaaac
    44461 tcccacatca aaggttagac catagacacg tttgaccaga taggattgac tggagacttc
    44521 catgatgagg tgggtacggt cattttgcac agcctgattc atcatgtcaa agaggtcaat
    44581 actctcaggg gttgtcaacg ctgacttaaa gaaagtctcg ccatcaagag ttgtgttcat
    44641 ggtcgacaac atagcaggtc tatgcccttg agataagatg ttataggcga aataggttgc
    44701 tgttgtctta cccttagtac cagtaaaggc aaggagtttg agtttctctt gtggattacc
    44761 atagaactcc atggcaatca aactcatggc tttctttata tcgttcacaa tgatgacagg
    44821 gatatcgact tcgtagtcct tttcagctac ataccaagct aaaccttgtg ttatagcaga
    44881 aagaaggtat tcttttttaa aggcagcgcc ttttgcaaaa aaaagagtgt cttctgttac
    44941 ttttcggctg tcgtagctga tgctatcaaa aacaactttg ctgtagttgt agtggtaatg
    45001 accttggtca ataatttcgc gaaaaaggcc atctttcttt aaaatatcta atacggtttc
    45061 aatcttaatc atactttcta ttgtaaaccg aaagtcgcaa atttacaagt aacaaggaaa
    45121 agtttataat ggaagataag gagtttttcc tagttatcaa aattgaatga ggaatctatg
    45181 tcgcacgaaa acaatcacca gcaggcccag atgttacggg ggactgcttg gctaacggct
    45241 agtaacttta tcagtcgcct actcggggct gtttacatta tcccttggta catctggatg
    45301 ggggcttatg cagctaaggc aaatggtctc tttaccatgg ggtacaatat ctatgcttgg
    45361 ttcttgttgg tttcaacagc ggggattcca gttgcggtgg ccaagcaagt tgccaagtat
    45421 aataccatgc gagaagaaga gcatagcttt gccctgattc ggagcttctt aggctttatg
    45481 acaggactag gcctggtttt tgctttagtc ttgtatgtct ttgctccttg gctagcagac
    45541 ttgtctggcg tgggcaaaga cttgatccca atcatgcaaa gcttggcttg gggagtcttg
    45601 attttcccgt ctatgagtgt tatccgagga tttttccaag ggatgaataa cctcaaaccc
    45661 tatgccatga gccaaattgc tgagcaggtc attcgtgtta tctggatgct cctagcaacc
    45721 tttatcatta tgaagctcgg ttcaggagat tatctagcag ccgttaccca atcaaccttt
    45781 gctgcctttg tcggtatggt agccagtttt gcagtcttga tttatttcct tgcccaagaa
    45841 ggttcactca aaagagtctt tgaaacagga gataagatta acagtaagcg tctcttggtt
    45901 gataccatta aggaagccat tccttttatc ctgacagggt ctgccatcca gctcttccag
    45961 attttggatc agctgacctt tatcaatagt atgagctggt ttaccaacta cagcaatgag
    46021 gacttggttg tcatgttttc ttatttctca gccaatccta ataaaatcac gatgattttg
    46081 atttctgtag gggtttcgat tgggagtgtt ggtttgccac ttttgacgga aaactatgtc
    46141 aagggggact tgaaagcagc ttctcgtctg gttcaggaca gtctcaccct actctttatg
    46201 ttcttgctac cagcaacggt tggagtggtt atggtaggag aacctcttta tacggtcttc
    46261 tatggtaagc cagatagttt ggctctgggc ttatttgtct ttgcagtttt gcagtctatt
    46321 attttaggct tgtacatggt cttgtctcca atgcttcagg ccatgttccg caaccgcaag
    46381 gccgttctct attttatcta tggttctatt gccaagctag tcttgcaact acctaccatc
    46441 gccctcttcc acagttatgg tcctttgatt tcaacaacca ttgctctcat cattcctaac
    46501 gtcttgatgt atcgggatat ttgtaaagta actggtgtca agcgcaaggt gattttgaag
    46561 cgaaccattt taatcagttt gctgacccta gtcatgtttc tgttaatagg aaccatccag
    46621 tggctgttag gatttttctt ccaaccaagt ggacgtttgt ggagcttctt ttatgtagct
    46681 cttgtcggtg ccatgggggg tggactttat atggttatga gtctgcgtac ctatttatta
    46741 gataaggtaa taggaaaagc ccaagcagat cgcctgcgag caaaatttaa gctttcgtaa
    46801 ggtagcattt ataaataaaa ggaataagtt gaacaatctt ttaggagaac ttgaaactta
    46861 ttcctttttt agcgtaaaaa tagctaaaat tatctttcaa aaatagaaaa tgaggttttt
    46921 ttcgattttt tggaaaaata attgactaaa caaaccaaac gttgtataat aaaacaaata
    46981 tttaaagcag gaggttctgg aaatgaaaaa gtctaagagc aaatatctaa ccttggcagg
    47041 tcttgtcctg ggtacaggag ttttattgag cgcgtgtgga aattctagca cggcgtcaaa
    47101 aacctacaac tatgtttatt caagtgatcc atctagcttg aactatctag cagaaaaccg
    47161 cgcagcaaca tccgatattg ttgcaaattt ggtagacggg ttattagaaa atgaccaata
    47221 tgggaatatt attccatcat tagcagagga ttggactgtt tctcaggacg gtttgaccta
    47281 tacctacaaa cttcgtaagg atgccaagtg gtttacttct gagggagaag aatatgcgcc
    47341 tgtaactgcc caggattttg tgacaggttt gcaatatgca gctgataaaa aatcagaagc
    47401 cttgtatcta gtgcaggact ctgttgctgg tttggatgac tatatcactg gtaaaacaag
    47461 cgacttttca actgtcggtg tcaaggcact tgatgaccaa acggttcaat atactttggt
    47521 taaaccagaa ctttactgga attcaaaaac acttgcaacg atactttttc ctgttaatgc
    47581 agatttcctg aaatcaaaag gggatgattt tgggaaggcg gatccatcta gtattttgta
    47641 caatggacct ttcttgatga aagcacttgt ctcaaaatct gctattgaat ataagaaaaa
    47701 ccctaattac tgggatgcta agaatgtctt tgtagacgat gtgaaattga cctactatga
    47761 tggtagcgat caagaatcac tggaacgtaa ttttacagct ggtgcttata ctacggctcg
    47821 tctttttcct aacagctcca gctatgaagg gattaaagaa aaatacaaaa acaatatcat
    47881 ctatagtatg caaaattcaa cttcatattt ctttaatttt aacctagata ggaagtctta
    47941 caattatact tctaaaacaa gtgacattga aaagaaatcg actcaggaag cagttctcaa
    48001 taaaaacttc cgtcaggcta tcaattttgc ttttgacaga acatcttatg gggctcagtc
    48061 tgaagggaaa gaaggtgcaa caaagatttt gcgtaaccta gtggttcctc caaactttgt
    48121 cagtatcaag ggaaaagact ttggtgaagt tgtagcctct aagatggtca actatggtaa
    48181 ggaatggcaa ggtatcaact ttgcggatgg tcaagaccct tactacaatc ctgagaaagc
    48241 caaggctaag tttgcggaag ctaagaaaga actcgaagca aagggtgttc aattcccaat
    48301 ccacttggat aagactgtgg aagtaacaga taaagtaggc atacaaggag ttagttctat
    48361 caaacaatca attgaatctg ttttaggttc tgataatgta gtgattgaca ttcagcaatt
    48421 aacatcagat gagtttgaca gttcaggcta ctttgctcaa acagctgctc agaaagacta
    48481 tgatttatat catggcggtt ggggacctga ttatcaagac ccgtcaacct atctcgatat
    48541 ttttaatact aatagtggag gagttctaca aaatcttgga ctagagcctg gagaagccaa
    48601 tgacaaggct aaggaagttg ggctggatgt ctatactcaa atgttggaag aagctaataa
    48661 agagcaagat ccagccaaac gttatgagaa atatgctgat attcaagctt ggttgattga
    48721 tagttcttta gttcttccaa gtgtttcgcg tgggggaaca ccatcattga gaagaaccgt
    48781 accatttgct gctgcctatg gtttaaccgg tacaaaaggg gttgaatcat ataaatacct
    48841 caaagtacaa gataagattg tcacaacaga cgaatatgca aaagccagag aaaaatggtt
    48901 gaaagaaaaa gaagaatcca ataaaaaagc ccaagaagaa ttggcaaaac atgtcaaata
    48961 aaatctaatt gtagataagt ttgtgtaaga agtctttgtg cttaaaaacg cataatatca
    49021 ggtatataaa atcctagatg ttatgcgttt tgttatagga agatttatta gattttatcc
    49081 tcaaattgag ttattcccga ctttatccgt tgaaatatta taaggagtat ttatgaaatt
    49141 aaaattatta agagtagata ctaaggtgat tatggggagt ttcttccttg ttctgtctag
    49201 tctacttgct ttgttgcttc cccttatctt aaaaggttta atagatggga gttctattga
    49261 aaatataggt tccaaagtat ttcaatcgtt tttgattttt attggtcaag ccttgttttc
    49321 ttctattggt tactatctgt ttagtcaatc gggtgaaaaa agatagcaaa aatcaggaaa
    49381 aaagtgatag aggggttgat ttatgcagag aaatccttct ttgataagag ccaaagtggg
    49441 gagttgactt ctgccattgt caatgacacg agtgtcattc gtgagttttt aattacgact
    49501 ttcccaaata ttattctgag tttagttatg gtacttggtt ccattgtagt cttatttagt
    49561 cttgattgga atctttctct acttttattc atcactcttc cttgtatgat gtttattatc
    49621 ttgccccttt ccaatatcag tgaaaagtat agtcgtcgtt tacaggagga aatcggattt
    49681 ttaacagggc aattgactga aaagattcaa gaacatgaat taattaagac taaccaagca
    49741 gagaaaagtg tacaggatgt tttggataat tgtattgaaa gggtacaaaa caattcactg
    49801 aaatcagata ggattacttc ttttgagacc ccgtttgctc tcttatttat ctttgcgact
    49861 atagctgtga tgctaaccta tgggggttat cgggtcagcg caggatatat atctgtggga
    49921 accttggttt cgtttttgat ttacctcttt caattactta atcctattag taatatagct
    49981 aattttgtaa ctgtttattc taggagcaag ggatcttcag ttgcactgga gaacttgctt
    50041 gcagttccta aagaaaaatt tgagggagga aaatcggtat caggacgagg gttgaatttt
    50101 aaccatgtct attttggtta tgatgaaaat cgacctgtct taaaggatat tatttgttca
    50161 attttcaagg ggcaaaaaat tgcttttgtt ggaccatctg gatcaggaaa atcaacgatt
    50221 gtgcgtttgt tagagcggtt ttataaaccg ctttcaggag atattctaat ggagcaatca
    50281 agtatatatg attttaactt aaaagaatgg agaagtaaaa tcgcttgggt ttcacaaaat
    50341 aatgcagtct tatctggcag tattcgtgac aatctttgtc tcggtttgaa tcgcttagta
    50401 actgatgatg aattgatgaa agtgctagac ttagtatcac taggtgatga gattcgctcc
    50461 atgaaagagg gactagatac tgaagttggt gaacgcggac gactcttgtc aggggggcaa
    50521 agccaaagac ttcaaatagc tagagcctac ttaaaagatg ctgaaattct tatatttgat
    50581 gaagccactg ctaatcttga tgcggattct gagtatgcga ttatcagtag cctctattct
    50641 gtattaaagg agaagacggt tgtgattata gcgcatcgtt tgtcaacggt aaaagatgtg
    50701 gattgtattt tcttcttaga ggaggggaaa atcactggct caggaactca taaggaacta
    50761 ctggaaaatc atgagcgtta tgctcgtttt gtgcaggagc aaatgataga gtgaaggtgt
    50821 cttttgagat tcaccatttt atagtctatt aaagggagca ggaaaaactc cctttttata
    50881 tagtttgaaa ctataactag ctcttgaaaa caagaaaacg aaggatacaa aataagtggt
    50941 acaatagtta ctatagattt ggaggaattg tatgagcaag gaattacaca ttaacacaat
    51001 tttagcacaa gcagggatca agtctgatga ggcaacaggt gctttggtga caccacttca
    51061 tttttcaacg acctatcagc atccagagtt tggtcgatct actgggtttg actatacgcg
    51121 cactaaaaat ccaactcgta gtaaggctga ggaagtcttg gcggctattg agtcagcaga
    51181 ctatgcccta gcgactagct cagggatgtc agctattgta ctggccttta gcgtctttcc
    51241 agtaggaagt aaggtcttgg cagtgcgtga tctttacggt ggttcttttc gctggtttaa
    51301 ccaagtggag caggaaggtc atttccattt tacctatgcc aatacagaag aagagttgat
    51361 tgccgagtta gaaaaggatg tggatgttct ctatatcgaa accccaacca atcccttgat
    51421 gttggaattt gatatcgaaa aactagcaaa attggctcat gctaagggtt ccaaagtggt
    51481 ggtggacaat accttctata gccctattta ccaacgtccg attgaagatg gagcagatat
    51541 cgttctccat tcagcaacca agtatctagc aggccacaat gatgtcttgg ctggagtggt
    51601 tgtgaccaat agtttagaac tatacgagaa gcttttttac aatcttaata caacaggggc
    51661 agtcttgtct ccatttgaca gctaccagtt gcttcgtggt ctcaagacct tgtctcttcg
    51721 tatggagcgc tcaacagcta acgcccaaga agtggttgcc tttttgaagg attctccagc
    51781 agttaaggaa gttctctaca ctggtcgtgg aggcatgatt tcctttaaag tagccgatga
    51841 aacacgcatt cctcatattt tgaacagtct caaggtcttc tcttttgcgg aaagtttggg
    51901 cggagtggaa agtcttatta cttatccaac gactcaaact catgctgata ttccagcaga
    51961 agtacgccat tcttatggtt tgacagatga cctcttgcgt ttgtctattg ggattgagga
    52021 tgctagagat ttgattgcag atttgcgcca agccttagaa ggataagaca aagatgggaa
    52081 aatatgattt tacaagcctg cccaaccgtt tagggcacca tacctataaa tggaaagaaa
    52141 cagaaacgga tagtgaagtt ctaccagctt ggatagcgga tatggacttt gtggtcttgc
    52201 ctgaaatccg ccaagccgtg caaacttacg cagaccaact ggtttatggt tatacctatg
    52261 ccagtgaaga cttaattaag gaagttcaaa agtgggaagc tacacaatac ggttacaact
    52321 ttgacaaaga ggctcttgtc tttatcgagg gtgtggtacc agccatctca acagctattc
    52381 aaacctttac aaaagaaggc gaggcggttt taattaacac gcctgtctac ccaccctttg
    52441 ctcgcagtgt caagttgaat aatcgtagat tgattactaa ttccttagtg gaaaaggata
    52501 gtctgtttga gattgacttt gaccaacttg aaaaggattt ggtggaagag gaggttaaac
    52561 tctatgttct ttgcaaccct cacaatcctg gtggacgtgt ttgggaaaaa gaagtgttgg
    52621 agaagattgg ccaactctgc caaaaacacg gtgttttgtt agtttcggat gagattcacc
    52681 aagatttgac cctctttggt cacaaacacc agtctttcaa taccatcaat cctgccttca
    52741 aaaattttgc tatcgtcttg agcagtgcca ctaaaacatt taatattgct ggaacaaaaa
    52801 attcctatgc agtcattgaa aatcctaagt tgagactggc tttccagaaa cgcctgttgg
    52861 ccaataatca gcatgaaatt tcaggcttgg gttatttggc gacagaagct gcctatcgat
    52921 acggtaaaga ttggctagag gaactcaagc aagtctttga agaccacatc aattatgtgg
    52981 tggatctatt tggaaaagag actaaaatca aggtcatgaa accgcaaggt acctacttga
    53041 tttggcttga cttttcagcc tatgacctga ctgatgaaac attgcaagag ttgttgagaa
    53101 atgaagccaa ggttatcctc aaccgtggtt tggattttgg agaggaagga agtctccatg
    53161 cccgcatcaa tatagctatg cccaaatctc tgttgcaaga agtctgtcag cggattgtgg
    53221 ctacttttgc caaatgttaa aaatccagcc ttctaggaga aaagtcttcc tagaaggcta
    53281 ttttcatagg cgaaaatatg gtataataaa aagataaggt aaaggtgaaa atatggctaa
    53341 attgattccg gggaaaattc gtatcgaagg tgttgccctt tatgaaactg gtaaggttga
    53401 tatcatcaag gaaaagaaca atcggctcta cgctcgcgtt gcagaagaag aactgcgcta
    53461 tagtttagag gatgatttgg ttttttgtgc ctgtgatttt tttcaaaaga ggggctactg
    53521 tgtgcatttg gcagcgctag agcattttct gaaaaatgat gagcgtggtc aggaaatctt
    53581 gtggagtctg gaagaaggtc atgaagaaaa agaggccgtt gaaaccaagg tgaccttggg
    53641 tggcaagttt ttggatcgaa ttttatctcc aaaatcagaa tgcgcctatg agttatcagc
    53701 tgtgggtcag gtggaagcag gaaccaatca tatcttgtgg actctgagaa tcggccaaat
    53761 caatagccaa aaatactatg tcattcggga tattccactt tttttaagga ttgtcgagca
    53821 gagaaagtcg tatatgattg gcaagattta tgaggagtct ctttcttggg aagcctttga
    53881 tgatgccagc caagaacttt tgactttctt gcacggacta acggaggaag ggctggttcc
    53941 agacctcttt ttccaaaacc aaggtcggca cctctttttc cctctgacct tttttgagca
    54001 gggtgtgaat ttactgatgg ccttgcccca ttttcaattt gaccatcaag tggatagcta
    54061 ccaaaccctg ctttttcagg atatgcatgc tgatgccaat ctatttgcct ttacagtaac
    54121 agaatattcg gactattttg aaatggaaat cagtgagagt cctagggtca atgtctttta
    54181 tcaaggagca gtacttttcc ataaaggtca ggtttatttt ttaacagacc agcagatgcg
    54241 tcttctcaag gaaattaaag cgttacctgt ggatcgacaa ggaaagaaat acctgcaatt
    54301 tgatagcagt gatcgagaca aattggcctc ctgtctgact ctttttatcc agatgggcac
    54361 cgtttcagct ccagaacgtc tacaaatcaa aacttttgcg ccctcttttt actttgacag
    54421 ggaagaagat aatcggattc gtttagaaat tcagtttgat tatggaaata gacaggtatc
    54481 tagccgacaa gagctagaag aattaccatt ttcaagtgac gcggacttag aagaaagaat
    54541 tttccaagtc tgtttggctg cgggctttga agccgatttt caatcttggc gtcaggcctt
    54601 aaaagcggag tctgtctatc atttttttca tgatatcatt ccagtctttg aaaaactcgg
    54661 gcatgttgac ctatcagata aactagaaga gctttatagc ctagcgagtc ctcaggtgca
    54721 gattgcctcc aagggaggtc tcttggaaat ccagtttgat tttcaagata ttgcccagga
    54781 ggaaatcgac caagccatac aggccttggt tgccaatcag gatttttata ttgattcgtc
    54841 taatcaagtt tactttttcg atgaagaaac caagaaaatt cgccaaaatc tacaggaact
    54901 ggggcaattt gaattaaaag atgggacctt gcaggctcgg aaatccttag cctacagttt
    54961 agctcatctc tttgaagggc gagaccgtgt ttctttttca caagaattcc agaatctggc
    55021 ccaggatttg acacatccag aggactttcc tttgcaagca actcaggtca agactgactt
    55081 gcgagattat caggaaaagg gaattggctg gttgcagatg ctccatcatt atggttttgg
    55141 tgggattttg gctgatgata tgggacttgg gaaaaccctt cagaccattg cttttttgac
    55201 tagtcaagtg acaaaagaaa gtcgagtctt gattctcgct ccgtcgggtt tgatttacaa
    55261 ctgggcggat gagtttcaga aatttgctcc acagttggat gtggctgttg ttcatggttt
    55321 gaaagcaagt cgtgaggaga ttcttgccga taaccatcaa atctatgtga ctagctatgc
    55381 caccttccgt caggacagtg agctttatca ggggatggcc tttgacttcc ttttcttaga
    55441 tgaagctcag gtcatgaaaa atgcccagac caagattgcc cagaccttga gacaatttgt
    55501 ggtgccgtcg gtctttgcct tgtcaggaac tccgattgaa aaccatctgg gtgagttgtg
    55561 gtctattttc caaatcgtca tgcccggact tttgccaagc aagaaagaat ttatgaaatt
    55621 gccagcagag cgagtggctc agtttatcaa gcctttcgtg atgcgacgca agaaagaaga
    55681 agttctgact gaattgccag acttgattga ggtgatttat aaaaatgaac tggaagacca
    55741 gcaaaaggct atttaccttg cccagttaca acagatgcga gaccatctgg ctcaagtgtc
    55801 agaacaggaa tttcagcgaa gtcgtgtgga aattttatct ggtttgatgc gcttgcgtca
    55861 aatatgtgac actcctgccc tgtttatgga agattatcag ggagccagcg gcaaactaga
    55921 tagtctccga gacctactgg tacaggtggc agacggcgga caccgtgtct tgattttctc
    55981 tcagttcaag ggaatgttgg aaaaaattga acaagaactg ccagacttgg gcctgacttc
    56041 ctttaaaatt acgggttcaa ccccagccaa ggaaagacaa gacatgacca aggcctttaa
    56101 ccaaggagaa agagatgcct ttctgatttc cctcaaggct ggtggggtcg gtctgaacct
    56161 gacaggtgca gatacagtga tcttggttga cctttggtgg aatcctgcgg tggaagcgca
    56221 agccattggc cgtgcccatc ggatgggtca ggaagaaacg gttgaggtct atcgcttggt
    56281 gaccaaggga accattgaag aaaaaattca ggaactccaa gaacaaaaga aacatctggt
    56341 gtcacaagta ttggacggca cagagtcacg tggtagtctg accctagcag aaattagaga
    56401 aattttggga atttctgaag ccagcacttg aaaaatcaag acaatacgct ataatgaagt
    56461 gttgaaagga aatagaaaat gaaacaagaa cgatttccat tggtgtcaga tgacgaggtc
    56521 atgttgactg aaatgccagt catgaatctc tatgatgagt ctgatctgat cagtaatatc
    56581 aagggtgagt atcgagataa aaattattta gaatgggctc ctattgctga agaaaaacca
    56641 gtaaaaccga ttgaaaagca agtagggaaa cctaaaaaag ctcctttagg ggttaaaaaa
    56701 gaaggaaaga gctatgcgga ggtggcgcgt gaagaagcgc gtgcagactt gaaaaagaaa
    56761 cgctctgcta actatctaac tcaggatttc agccttgcga gacgtcattc tcagcccagt
    56821 ctagttagac agggcaatca accgacaact cctttccaaa aggaaaatcc tggtgaattt
    56881 gtcaaatata gccaaaaatt gacccagtct cattatatct tggcggaaga agttcattct
    56941 atccctacca agaatgaaga agtgtcagca cctgctccaa agaaaaacaa ttatgatttt
    57001 ctaaagaaga gccaaatcta caataaaaaa agtaaacaaa cagaacaaga acgtcgggtt
    57061 gcccaagagt tgaatctgac cagaatgaca gaatagggga gaaaacatgt caaagacata
    57121 tcattttatc ggaattaagg gatcagggat gagtgccttg gccttgatgt tgtaccagat
    57181 ggggcacaag gttcagggat cagatgttga aaagtactac tttacccaac gcggtcttga
    57241 gcaggcagga attaccattc ttccttttga tgaaaagaat ctagacggtg atatggaaat
    57301 tatcgctgga aatgcctttc gtccagataa caacgtcgaa attgcctatg cggaccaaaa
    57361 tggtatcagc tacaaacgtt accatgagtt tctaggtagc tttatgcgtg actttgttag
    57421 catgggagta gcaggagcac atggaaaaac ttcaacgaca ggtatgttgt ctcatgtctt
    57481 gtctcacatt acagatacca gcttcttgat tggagatggg acaggtcgtg gttcggccaa
    57541 tgccaaatat tttgtctttg aatctgacga atatgagcgt cacttcatgc cttaccaccc
    57601 agaatactct attatcacca acattgactt tgaccatcca gattatttca caagtctcga
    57661 ggatgttttc aatgccttta acgactatgc caaacaaatc accaagggtc tttttgtcta
    57721 tggtgaagat gctgaattgc gtaagattac gtctgatgca ccaatttatt attatggttt
    57781 tgaagctgaa ggcaatgact ttgtagctag tgatcttctt cgttcaacaa ctggttcaac
    57841 cttcaccgtt catttccgtg gacaaaactt ggggcaattc cacattccaa cctttggtcg
    57901 tcacaatatc atgaatgcga cagccgttat tggtcttctt tacacagcag gatttgattt
    57961 gaacttggtg cgtgagcact tgaaaacatt ttccggtgtt aaacgtcgtt tcactgagaa
    58021 aattgtcaat gatacagtga ttatcgatga ctttgcccat catccaacag aaattattgc
    58081 gaccttggat gcggctcgtc agaaataccc aagcaaggaa attgtagcag tctttcaacc
    58141 gcataccttt acaagaacca ttgccttgtt ggacgacttt gcccatgctt taaaccaagc
    58201 agatgctgtt tatctagcgc aaatttatgg ctcggctcgt gaagtagatc atggtgacgt
    58261 taaggtagaa gacctagcca acaaaatcaa caaaaaacac caagtgatta ctgttgaaaa
    58321 tgtttctcca ctcctagacc atgacaatgc tgtttacgtc tttatgggag caggagacat
    58381 ccaaacctat gaatactcat ttgagcgtct cttgtctaac ttgacaagca atgttcaata
    58441 ggatgttccc atggaaattc caattaagat cattcaggca agcaagtctg atttgcctga
    58501 gataggggca cttcagacct cgtcttttcc agctgaaaag cagcaacttt cccatatttt
    58561 agaagaaagt atccgtaagt gtgcggatac ctttcttcta gctagggatg aaaatcaact
    58621 tttaggctat attttatcaa gtccccagtc agacaatccg caatatctaa aagtacattc
    58681 tttagtcatc gagtctgacc atcagagaca gggcttggga acacttcttc ttgcagcctt
    58741 gaaagaggtg gcagttgagc tggattacaa agggattcgt ttggagagtc ctgatgagct
    58801 gctttcctat tttgaaatga acggttttgt tgatgaagaa gcaactttgc tctatgcaac
    58861 tagccagggc tatagtatga tttggtttaa tcccttttat ctggaggaac aatgaaaatc
    58921 agaaaagcaa gattagaaga tttggatcgg attgttgaac ttgaatttga aaatttttcg
    58981 gtcgaagaag ccattcctcc ttctgtcttt gaagcacatt tgagagaaat tcagacctct
    59041 tttctggttg ctgaaaaaga aggaagaatc atgggttata tcgaaggacc agttggcctg
    59101 caccgccatc tgcaagacca gtcttttaca gaagaaataa aagactatag tcatgagcct
    59161 ggtggttata tctctgtgac ctgtctgtct attgccaagg aggcacaggg actcggactg
    59221 ggtcagaaat tgctgacagc cttgaaagaa gttgctcttg aagatgagag agatggcatt
    59281 aatctaacct gtcatgacta tctcatcgcc tactatgaaa aacatggatt tgtcaacgaa
    59341 ggccagtccc agtcaacctt tgcaggggaa acatggtatg atatggtctg ggaaatgaaa
    59401 aaataagtta ggaaaagtat catagattgc tttttttgga cttttaagat ataatattat
    59461 ggattatcaa caaggaggaa aaacttttga gtgaaaagtc aagagaagaa gagaaattaa
    59521 gctttaaaga gcagattctg agagatttag aaaaagtaaa aggctatgat gaagttctga
    59581 aagaagatga ggcagtagtt cgcactcctg caaatgaacc ttcaactgaa gaactcatgg
    59641 ctgattcctt gtcaacggta gaggagatta tgagaaaagc tcctaccgtg cctactcacc
    59701 caagtcaagg tgtaccagct tctccagcag atgagattca aagagaaact cctggtgttc
    59761 caagtcatcc aagtcaagat gtaccttctt ctccagcgga agaaagtgga tcaagaccag
    59821 gtccaggtcc tgttagacct aagaaacttg aaagagaata caatgaaacc ccaacaaggg
    59881 tagctgtttc ctatacgacg gcagagaaaa aagcagaaca agcaggtcca gaaacaccta
    59941 cgcctgctac agaaacagtg gatatcatca gagatacatc acgtcgtagc cgtagagaag
    60001 gagcaaaacc cgttaagcct aagaaagaga agaagtcaca tgtgaaagct tttgtgattt
    60061 cattccttgt attccttgcc ttgctctcag caggtggtta ctttggttac cagtacgtgc
    60121 tagattcctt attacctatc gatgctaatt ctaagaaata tgtgacggtt ggaattccag
    60181 aaggttcaaa cgttcaagaa atcggtacga cgcttgaaaa agctggtttg gtaaagcatg
    60241 gtctgatttt tagtttttat gccaagtata aaaattatac cgacttgaaa gcaggttact
    60301 acaatttgca aaagagtatg agtacagaag acttactcaa agagttgcaa aaaggtggaa
    60361 cagatgaacc gcaagaacct gtacttgcga ctttgacaat tccagaaggt tataccttgg
    60421 atcagattgc tcaagctgtg ggtcaattgc aaggtgactt caaagagtct ttgacagcgg
    60481 agactttctt ggctaaagtt caagatgaga cgtttatcag tcaagcagta gcgaaatatc
    60541 ctactttact ggaaagtttg cctgtaaaag acagcggtgc gcgttatcgt ttggaaggat
    60601 accttttccc agctacatac tctatcaagg aaagcacaac tattgagagc ttgattgatg
    60661 agatgttagc tgctatggat aagaacctat ctccttacta tagtactatc aaatctaaaa
    60721 acttgactgt caatgagttg ttgaccattg cttccttggt cgaaaaagaa ggtgccaaga
    60781 cagaagatcg taagctcatt gcaggtgtat tctacaatcg tttgaatcgt gatatgccac
    60841 ttcaaagtaa tattgcaatc ttgtatgccc aaggaaaact ggggcaaaat atcagtctag
    60901 ctgaggatgt tgcgattgat accaacattg attcacctta taatgtttat aaaaatgtag
    60961 gtctcatgcc tggtccagtc gatagtccaa gtctggatgc gattgagtca agcatcaatc
    61021 aaactaagag cgataacctc tactttgtag cagatgtcac agaaggcaag gtctactatg
    61081 ctaacaatca agaagaccac gaccgcaatg tcgctgaaca tgtcaacagc aaattaaact
    61141 aaacaaacta aaattatgtt atacttcaca taattttctt tagaaaatat tatcagaaga
    61201 aagttgagaa aaatggcaga aaaaacatat cctatgaccc ttgaggaaaa ggaaaaactt
    61261 gaaaaagaat tagaagaatt gaaattggtt cgtcgaccag aagtggtaga acgcattaag
    61321 attgcccgtt catacggtga cctttcagaa aacagtgagt acgaagcagc taaggatgaa
    61381 caagcctttg tcgaaggaca aatctctagc ttagaaacaa aaatccgcta tgctgaaatc
    61441 gtcaatagcg acgcagttgc ccaggacgaa gtagcgattg gtaaaacagt caccatccaa
    61501 gaaattggtg aggacgaaga agaagtttat attatcgtag gttcagctgg tgcggatgcc
    61561 tttgcaggta aggtttcaaa tgaaagccca attgggcagg ccttgattgg caagaaaaca
    61621 ggtgatacag caaccattga aacgcctgtt ggtagctatg atgtaaaaat cttgaaggtt
    61681 gaaaaaacag cctaaaaaca gaaaaaggag tggggaggcg atgtgcttca ctcactcctt
    61741 tttccatttt gctattcttc gaaaatctct tcaaaccacg tcagcgtcgc cttgccgtat
    61801 gtatggttac tgacttcgtc agtttcatct acaacctcaa aaccatgttt tgagccgact
    61861 tcgtcagttt catctacaac ctcaaaacca tgctttgagc tgacttcgtc agtttcatct
    61921 acaacctcaa aaccatgctt tgagctgact tcgtcagttt catctacaac ctcaaaacca
    61981 tgctttgagc tgacttcgtc agttctatct acaacctcaa aacagtgttt tgagcaacct
    62041 gcggctagtt tcctagtttg ctctttgatt tttattgagt attagatgta tagtgcattg
    62101 aagttaaaat agtacacttt gatttctaag acattgttag aaattagatt aaattcctta
    62161 atcgatttgc gcatgtttta tttcatttca ctatacttta gattggaaaa taaggaatca
    62221 cataaatcgc aagaaatagg gaatctgatt cgtgtttata accgttcaaa aagagaagag
    62281 gctgaaagtg agccacttaa tctttatgtc gaagatgaaa agggcaatct cctggcaggt
    62341 ttgatagcag agacttttgg aaattggcta gaaatcgagt atttgtttgt aaaagaggaa
    62401 ctgcgagggc aaggaatcgg ttcaaaacta ttgcaacagg cagaaagtga agctaagaat
    62461 cgaaattgtt gttttgcctt tgtcaatact taccagttcc aggcaccgga cttttatcag
    62521 aaacatggct acaaggaagt tttttctttg caagactatc tctacattag gcaaagatat
    62581 tattaccaaa agaatctgta aggtgagtat ggaggtataa tacaaagaga ggctcgcaca
    62641 taagtatgga ggtggactga tgtatatgag taactatcga taaataccag ttataaccta
    62701 gaaattgtgt ctttctagac gatattgagg acaatataat cgtagcccca aaattaggaa
    62761 tcaagactta tcaggttaag aaaagaagtg atgttgttga tattttaaac aatttattta
    62821 aactaaaaac tctctatcta ttaatccgag attcccctct cggattttgt gttgctttca
    62881 ttaaatttac aggtaagact tgctagcttc agtaaatata cacaagtttt tcagaataga
    62941 gtgaaatcca aaagaaaatg gaaataatga aagctcttgt aggcataaga aaacacctct
    63001 gtgttatact tgttgttcaa ccacaaacac aagaaaggca cagagatgca agacaattat
    63061 actacaaaag ccaaacattt gacaatcgat agccgtcgct taatcgaaag atggaaaaaa
    63121 gaagggaaat caaatagaga aattgcctct ctgcttggaa aagttcctca aactatccac
    63181 actgaaatca agcgtgggac agtccgacaa tgtcttggaa aagggcgctt caaagaggtt
    63241 tattctgccg actatgctca acagtcttat gaaaataatc gcaagcgctc ggtcaagaaa
    63301 tcaagcttga ccaaggaact aaaggaaaag attctccact atcataacca aaaattttcg
    63361 cctgaaatga tggtgatggc taaaggggtt aacgtgggaa tttcaaccat ttactattgg
    63421 attcatcatg gaaaattggg gttaagcaaa caggatctgc tttaccctag aaaaggaaaa
    63481 gcgcttaaga aacaggctag caccaacttt aaacctgctg gtcaatccat cgaacagcgg
    63541 tctgaagcta tcaatcttcg cttggagaat gggcattatg agattgatac ggttctactt
    63601 acgagagcga aaaactactg cttgcttgtc ttgacggatc gaaagagtag acatcagatc
    63661 atccgattga ttccaaataa aagtgctgag gtggtcaatc aggctctaaa actcatctta
    63721 aaacaacaca agattctttc catcacggca gataatggaa cggaattcaa tcgcttgttt
    63781 gatgtatttt ctgaggagca catctattat gcgcacccct atgcctcttg ggaaagggga
    63841 actaatgaga atcacaacag gctcattcgt agatggttac ctaagggaac caagaaaatg
    63901 actcccaaag aagtcgcatt catcgaaaag tggattaaca actatcctaa aaaatgcttg
    63961 gactacaagt cacccagaga agacttctgg atggctaact tgaacttgaa atttagcaaa
    64021 atggaaataa tttttattaa acgcttccaa tagcggttaa aagttgacaa atttaaattt
    64081 taggactaca ctaagtaagt aaattttatt ttaaaggaga atttgttatg aatttaacat
    64141 ttttaggctt atgtattgcc tgtatgggcg tatctgtcgg tgaaggttta ttgatgaatg
    64201 gactgtttaa atcagtagca cgccaaccag atatgctttc tgagtttcgt agtttgatgt
    64261 ttttaggtgt tgcctttatt gaaggaactt tctttgtaac tcttgtcttc tcatttatta
    64321 tcaaataaat acatggaacg agaagaaaag ggaggatttt agatggaaga aagtattaat
    64381 ccaatcatct ctattggtcc tgttatcttc aatctgacta tgttagccat gactttgttg
    64441 attgtgggag ttatttttgt ctttatttat tgggcaagcc gcaatatgac cttgaaaccc
    64501 aaaggaaagc aaaatgtact tgagtatgtc tatgactttg ttattggatt tacagaacct
    64561 aacattggtt cgcgctacat gaaagattac tcactctttt tcctttgttt attccttttc
    64621 atggtgattg ccaataacct tggcttaatg acaaagcttc aaacgatcga tgggactaac
    64681 tggtggagtt cgccaaccgc taatttacag tatgacttaa ccttatcttt tcttgtcatt
    64741 ttgttgacac atatagaaag cgttcgtcgt cgtggattta aaaaaagtat aaaatctttt
    64801 atgagtcctg tttttgtcat accgatgaat atcttggaag aatttacaaa cttcttatct
    64861 ttggctttgc ggatttttgg gaatatcttt gcaggagagg tcatgacgag tttgttactt
    64921 cttctttccc accaagctat ttattggtat ccagtagcct ttggagctaa tttggcttgg
    64981 actgcatttt ctgtctttat ttcctgcatc caagcttatg tttttactct tttgacatct
    65041 gtgtatttag ggaataagat taatattgaa gaggaataga aaggagtaac tgatgcacgt
    65101 aacagtaggt gaattaattg gtaattttat tttaatcact ggctctttta ttcttttgct
    65161 agtcttgatt aaaaaatttg catggtctaa tattacaggc attttcgaag aaagagctga
    65221 aaaaattgct tcagatattg acagagctga agaagcccgt caaaaagcag aagtattggc
    65281 tcaaaaacgc gaagatgaat tggctggtag ccgtaaagaa gctaagacaa tcattgaaaa
    65341 tgcaaaggaa acagctgagc aaagtaaggc taatatctta gcagatgcta aactagaagc
    65401 aggacactta aaagaaaaag ccaatcaaga aattgctcaa aataaagtag aagctttaca
    65461 gagtgttaag ggtgaggtcg cagatttgac catcagctta gctggtaaaa tcatctcaca
    65521 aaaccttgac agtcatgccc ataaagcact cattgatcag tatatcgatc agctaggaga
    65581 agcttaatgg acaagaaaac agtaaaggta attgaaaaat acagcatgcc ttttgtccaa
    65641 ttggtacttg aaaaaggaga agaagaccgt atcttttcag acttgactca aatcaagcaa
    65701 gttgttgaaa aaacaggtct gccttctttt ttaaaacaag tggcagtaga cgagtcggat
    65761 aaggaaaaaa caattgcttt tttccaagat tctgtgtcgc ctttattaca aaactttatc
    65821 caggttctgg cctacaatca cagagcaaat cttttttatg atgtgcttgt agattgcttg
    65881 aaccgacttg aaaaagaaac aaatcgattt gaagtgacga ttacgtctgc tcatcctcta
    65941 actgatgaac agaagactcg tttgctccct ttgattgaga aaaaaatgtc tctgaaagta
    66001 aggagtgtaa aagaacaaat cgatgaaagt ctcattggtg gttttgtcat ttttgccaat
    66061 cacaagacaa ttgatgtgag tattaaacaa caacttaaag ttgttaaaga aaatttgaaa
    66121 tagaaagtgg tgttcttttg gcaattaacg cacaagaaat cagcgcttta attaagcaac
    66181 aaattgaaaa tttcaaaccc aattttgatg tgactgaaac aggtgttgta acctatatcg
    66241 gggacggtat cgcgcgtgct catggccttg aaaatgtcat gagtggagag ttgttgaatt
    66301 ttgaaaacgg ctcttatggt atggctcaaa acttggagtc aacagacgtt ggtattatca
    66361 tcctaggtga ctttacagat atccgtgaag gcgatacaat ccgccgtaca gggaaaatca
    66421 tggaagtccc tgtaggtgaa agtctgattg gtcgtgttgt ggatccgctt ggtcgtccag
    66481 ttgacggtct tggagaaatc cacactgata aaactcgtcc agtagaagca ccagctcctg
    66541 gtgttatgca acgtaagtct gtttcagaac cattgcaaac tggtttgaaa gctattgacg
    66601 cccttgtacc gattggtcgt ggtcaacgtg agttgattat cggtgaccgt cagacaggga
    66661 aaacaaccat tgcgattgat acaatcttga accaaaaaga tcaagatatg atctgtatct
    66721 acgtcgcgat tggacaaaaa gaatcaacag ttcgtacgca agtagaaaca cttcgtcagt
    66781 acggtgcctt ggactacaca atcgttgtga cagcctctgc ttcacaacca tctccattgc
    66841 tcttcctagc tccttatact ggggttgcta tggcggaaga atttatgtat caaggtaagc
    66901 atgttttgat tgtatacgat gatctatcaa aacaagcggt agcttatcgt gaactatcgc
    66961 tcttgcttcg tcgtcctcca ggtcgtgaag ccttcccagg ggatgttttc tatctccaca
    67021 gccgtttgct tgagcgctca gctaaagttt ctgatgaact tggtggtgga tcaattacag
    67081 ccctaccatt tatcgagaca caagcaggag atatctcagc ctatatcgca accaacgtga
    67141 tttctatcac tgatggacaa atcttccttg gcgatggcct cttcaatgca ggtattcgtc
    67201 cagccatcga tgcgggttca tctgtatctc gtgtaggtgg ttctgcacaa atcaaagcca
    67261 tgaagaaggt tgctggtaca cttcgtatcg accttgcttc ataccgtgag ttggaagcct
    67321 ttactaagtt tggttctgac ttggacgcag caacacaggc taagttgaac cgtggacgtc
    67381 gtaccgttga ggtcttgaaa caacctgttc acaaaccatt acctgttgag aaacaagtaa
    67441 ccattcttta tgctttgaca catggtttct tggatactgt tccagtagat gatattgttc
    67501 gtttcgagga agagttccat gccttctttg atgctcaaca tccagagatt ttggaaacca
    67561 ttcgtgatac aaaagacttg ccagaagaag cagtcttgga tgctgcgatt acagagtttc
    67621 tcaatcaatc tagcttccaa taagaataga ggtgtcagat ggcagtgtct ctaaatgata
    67681 ttaaaacaaa aatcgcctca acaaaaaata cgagtcaaat cactaatgcc atgcaaatgg
    67741 tatcggctgc taagttaggt cgctctgaag aagctgctcg caacttccaa gtttacgctc
    67801 agaaagtgcg taaacttttg acagatatcc ttcatggtaa tggagctggt gcttcaacta
    67861 atccgatgtt gattagccgt tctgtgaaga agacaggcta tatcgttatc acttcagacc
    67921 gcggtttggt tggaggttat aattcctcta ttttgaaagc tgttatggag ttgaaagaag
    67981 aataccaccc agacggtaaa ggttttgaaa tgatctgtat cggtgggatg ggagctgatt
    68041 tctttaaggc tcgcggtatt caaccacttt atgaattacg tggcttggca gaccaaccta
    68101 gctttgatca agttcgtaag attatttcaa aaactgttga aatgtaccaa aatgaactct
    68161 ttgatgagct ttatgtttgc tacaaccacc atgtcaatac gctaaccagt caaatgcgtg
    68221 tggaacaaat gcttccgatt gttgacttgg atccaaatga agcggatgaa gagtacagct
    68281 tgacttttga attggaaacc agccgagaag aaattctgga gcagttgttg cctcagtttg
    68341 cagaaagtat gatttacggt gccattatcg atgccaagac agctgagaat gctgcgggta
    68401 tgacagccat gcaaacagcg acagataatg ctaagaaagt catcaatgat ttgacaattc
    68461 agtataaccg tgccagacag gcggcgatta cacaagaaat tacagaaatc gtagcaggtg
    68521 ctagtgcctt agaataggct ctagtccagc tcgtatgaaa atgaacttag gacctagttg
    68581 agctaggaac cgacagtatc ttatatagaa taggagaagg agatgagttc aggtaaaatt
    68641 gctcaggtta tcggtcccgt tgtagacgtt ttgtttgcag caggggaaaa acttcctgag
    68701 attaacaatg cacttgtcgt ctacaaaaat gacgaaagaa aaacaaaaat cgtccttgaa
    68761 gtagccttgg agttaggaga tggtatggtt cgtactatcg ccatggaatc aacagatggg
    68821 ttgactcgtg gaatggaagt attggacaca ggtcgtccaa tctctgtacc agtaggtaaa
    68881 gaaactttgg gacgtgtctt caacgttttg ggagatacca ttgacttgga agctcctttt
    68941 acagaagacg cagagcgtca gccaattcat aaaaaagctc caacttttga tgagttgtct
    69001 acctcttctg aaatccttga aacagggatc aaggttattg accttcttgc cccttacctt
    69061 aaaggtggta aagttggact tttcggtggt gccggagttg gtaaaactgt cttaatccaa
    69121 gaattgattc acaacattgc ccaagagcac ggtggtattt cagtatttgc tggtgttggg
    69181 gaacgtactc gtgaggggaa tgacctttac tgggaaatga aagaatcagg cgttatcgag
    69241 aaaacagcca tggtctttgg tcagatgaat gagccaccag gagcacgtat gcgtgttgcc
    69301 cttactggtt tgacaatcgc tgaatacttc cgtgatgtgg aaggccaaga cgtgcttctc
    69361 tttatcgata atatcttccg tttcactcag gctggttcag aagtatctgc ccttttgggt
    69421 cgtatgccat cagccgttgg ttaccaacca acacttgcta cggaaatggg tcaattgcaa
    69481 gaacgtatca catcaaccaa gaagggttct gtaacctcta tccaggctat ctatgtgcca
    69541 gcggatgact atactgaccc agcgccagca acagccttcg ctcacttgga ttcaacaaca
    69601 aacttggaac gtaagttggt acaattgggt atctacccag ccgttgaccc acttgcttca
    69661 agctcacgtg ccttggcacc tgaaatcgtt ggagaagagc actatgcagt tgctgctgaa
    69721 gtaaaacgtg tccttcaacg ttaccatgaa ttgcaagata tcattgctat ccttggtatg
    69781 gatgagcttt ctgatgaaga aaagaccttg gttgctcgcg cccgtcgtat ccagttcttc
    69841 ttgtcacaaa acttcaacgt tgcggaacaa tttactggtc agccaggttc ttatgttcca
    69901 gttgctgaaa ctgtacgtgg ctttaaggaa atccttgatg gtaaatacga ccacttgcca
    69961 gaagatgcct tccgtggtgt aggttctatc gaagatgtga ttgcaaaagc tgaaaaaatg
    70021 ggattttaag aggtgatcta tggctcagtt aactgtccag atcgtgacac cagatggtct
    70081 cgtctatgat caccatgcca gctatgtatc ggttcgaact ctggatggtg agatggggat
    70141 cttgccacga catgaaaata tgattgcggt tttagcagtt gatgaagtaa aggtaaaacg
    70201 tatcgatgat aaagatcacg tgaactggat tgcagtaaac ggtggcgtta ttgaaattgc
    70261 caatgatatg atcacaatcg tcgctgactc tgcagaacgt gctcgtgata tcgatatcag
    70321 tcgtgcagaa cgtgccaaac ttcgtgcaga acgtgcaatt gaagaagcac aagacaaaca
    70381 tttgattgac caagaacgtc gtgctaagat tgctttgcaa cgtgctatta accgtattaa
    70441 tgtcggaaat agactataag aaaaaatgaa cttgaaaata ccaagttcat tttttatggt
    70501 gttttaagga gcaaaacgga tgcagactgc ttcgggaaca tggaagtcgt tggagagttc
    70561 tgctagacga ccattgtcac aattacgttt aaagacagtt gcattgtcag agtcttgatg
    70621 gacaacaatg agaaattttt ggtcgggtgt caaatcaaaa tcacgtggag tctgaccatg
    70681 cgttggaacg atttctaata actctaagct accgtccgca aggatggtat atactgcgat
    70741 agaatcatgg ccacggttag aagcgtagag gtatttaccg tctttagaga gatgaatagc
    70801 agcggttcca ttaaagcctt cgtaagcttc cggtaaagtt gaaatgacct gcatacgttc
    70861 aaattcgcca acgccatcgt agattaaaac ttcgatagta ctattgagtt cacaaatgag
    70921 ataagcgatt ttatagtggt tatggaaaat gatatggcgt gagcctgctc ctggcttgct
    70981 gtgataggta tagagcttag ataattttcc ttcttgatcg aggtcatagg tgatgacttg
    71041 gtcagttccc aagtcgcagg tcactagata gtggtcaggt gttaaatctg tatagtgaac
    71101 atggggggaa gcttgatttt catgtggacc ttggccactg tgttgatcca tatcactaag
    71161 tagaagacta ccatcttcct ggcgtttata aacaaggact tgtcccttgt gatagttagc
    71221 tgcgtaaacc aaatcacgct tttcatcgac agcaacataa cagtggggag ctccttcttc
    71281 aacaacatga tttaacacag tcccgtcagt ttgataggct gcaattcccc ccttatcgtc
    71341 ttggctacca acagtgtata aatgttggtg ctggtcaaag gcaaggtagg ttggacttgg
    71401 ctcagctgca aaaagttcta gatttgaaag ctgaccagtt tctgtatcaa agtctgcctt
    71461 gtaaatccct tgagaagtac gacgtgtata agttccaaaa taaacagttt ctttcattac
    71521 tatacctctg tgtaaagata agactattat atcacaaaaa caagtaaatt aaagatatcc
    71581 aattagatgt aagcacttta aaaaagagtt attttgtttc aaaaatggta taatgagaga
    71641 acaatagaaa ggaagtattt atggagcaaa aagagaaaca ttttagccta tcttggtttt
    71701 tcaagtggtt tttagataac aaggcaatta cggtattttt agtaacctta ttattgggac
    71761 tgaatctttt tattttaagt aagattagtt ttctattttc acctgtttta gactttttag
    71821 cagttgtgat gttgccagtc attttgtctg gtttgttata ttatttgttg aatcctattg
    71881 ttgattggat ggagaagcat aaggttaatc gtgttatagc tatcactatt gtctttgtta
    71941 tcatcgctct ctttatcatt tggggcttgg cagtcgccat tccaaatctg caacgtcagg
    72001 ttttgacctt tgcaagaaac gttcctgttt acttagaaga tatagatagg attgttaatg
    72061 gattggtagc ccagcacctg ccagatgatt tcagacctca attagagcaa gttttgacca
    72121 atttttctag ccaggctaca gttttggcaa gtaaggtttc atctcaggca gtcaactggg
    72181 tgagtgcctt tattagcggg gcttctcaag tgattgttgc cttgattatc gttcctttca
    72241 tgctctttta tctcttgcgt gatgggaaag gcttgcgtaa ctatttgacc caattcattc
    72301 caagaaaatt gaaggaacct gttggacaag ttttatcaga tgtgaatcaa cagttgtcca
    72361 actatgttcg agggcaagtg acagtggcta ttattgtagc agtaatgttt atcatcttct
    72421 tcaagattat tggtctacgc tatgcggtta cgctgggggt tactgctggt attttaaatc
    72481 tggtccctta tcttggtagc tttctagcca tgcttcctgc tctagtattg ggtttgattg
    72541 ctggtccagt catgcttttg aaagtagtga ttgtctttat cgtagaacaa actattgaag
    72601 gccgttttgt ctctccattg attttgggaa gtcaattaaa catccaccct attaatgttc
    72661 tctttgtttt gttaacttca ggatctatgt ttggtatctg gggagtttta cttggtattc
    72721 cggtttatgc ctctgctaag gttgtcattt cagccatttt cgaatggtat aaggtagtca
    72781 gtggtctata tgaattagag ggtgaggaag tcaagagtga acaatagtca acagatgtta
    72841 caggctttgg aggagcaaga tttaactaag gctgagcatt atttcgccaa agctttagaa
    72901 aatgattcaa gtgatcttct gtatgaattg gcaacttatc ttgaagggat tggtttctat
    72961 cctcaggcca aggaaattta cctgaaaatt gtagaggatt ttccagaggt tcatcttaat
    73021 ctagctgcaa ttgctagcga ggatggtcaa atagaagaag cctttaccta tcttgaggaa
    73081 atccaagctg acagtgactg gtatgtctcg tctttggctc tgaaggcaga cctttaccag
    73141 ctggaaggtt tgacagatgt ggcacgtgag aaattattgg aggccttgac ctactcagag
    73201 gattctctct tgatattggg tttggcagag ttggatagtg agttggaaaa ttaccaagcg
    73261 gctattcaag cctatgccca gttagataat cgctcgattt atgagcaaac gggcatttcc
    73321 acctatcaac gaattggctt tgcctatgct cagttaggga aatttgaaac ggctactgag
    73381 tttttagaaa aagccctgga gttagaatac gatgacttaa cagcttttga gttggccagt
    73441 ctttattttg atcaagaaga atatcaaaaa gccaccctct actttaagca gcttgatacc
    73501 atttctcctg actttgaagg ctatgagtat gggtacagtc aggctttaca taaggaacat
    73561 caagttcaag aagccctgcg tatcgctaag caaggattag agaaaaatcc ctttgaaact
    73621 cgcctcttgc tagctgcttc acaattttct tatgaattgc atgatgctag tggtgcagaa
    73681 aattatctcc ttactgcaaa agaagacgct gaggatacag aagaaatctt gcttcgttta
    73741 gccactattt atctggagca ggagcgttat gaggatattc tagaattgca gagtgaggag
    73801 ccagaaaatc ttttgaccaa gtggatgatt gctcgttctt atcaagaaat ggacgatttg
    73861 gatactgctt atgagtatta tcaagagttg acaggagatt tgaaggacaa tccagaattt
    73921 ctggaacact atatctatct cttgcgtgaa ttgggacatt ttgaagaagc aaaagtccat
    73981 gctcacactt acttaaaact ggttccagat gatgtgcaaa tgcaagaact gtttgagaga
    74041 ttgtaagaat g
//
LOCUS       DAVHEX010000003        66085 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVHEX010000003 DAVHEX010000000
VERSION     DAVHEX010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 66085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66085
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.003"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(295..2022)
                     /gene="ezrA"
                     /locus_tag="VVL45_000164"
     CDS             complement(295..2022)
                     /gene="ezrA"
                     /locus_tag="VVL45_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEU4186426.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLEAGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2104..4050)
                     /gene="gyrB"
                     /locus_tag="VVL45_000165"
     CDS             complement(2104..4050)
                     /gene="gyrB"
                     /locus_tag="VVL45_000165"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEU4186427.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEIVGDTDKTGTTVHFTPDPKIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4065..4628)
                     /locus_tag="VVL45_000166"
     CDS             complement(4065..4628)
                     /locus_tag="VVL45_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU4186428.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEDISNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4713..5267)
                     /locus_tag="VVL45_000167"
     CDS             complement(4713..5267)
                     /locus_tag="VVL45_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEU4186429.1"
                     /translation="MVKVAVILAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            complement(5278..6501)
                     /locus_tag="VVL45_000168"
     CDS             complement(5278..6501)
                     /locus_tag="VVL45_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU4186430.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVPLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6538..8988)
                     /locus_tag="VVL45_000169"
     CDS             complement(6538..8988)
                     /locus_tag="VVL45_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEU4186431.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLRKKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTEASLEPRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEDFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPSVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIVGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9188..10117)
                     /locus_tag="VVL45_000170"
     CDS             complement(9188..10117)
                     /locus_tag="VVL45_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEU4186432.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELIVAVNALMHQDMMDRIKPFLNFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARSLIQEGNFSH
                     AQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVR"
     gene            10257..10544
                     /locus_tag="VVL45_000171"
     CDS             10257..10544
                     /locus_tag="VVL45_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEU4186433.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10578..11912)
                     /gene="ciaH"
                     /locus_tag="VVL45_000172"
     CDS             complement(10578..11912)
                     /gene="ciaH"
                     /locus_tag="VVL45_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HEU4186434.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(11902..12576)
                     /gene="ciaR"
                     /locus_tag="VVL45_000173"
     CDS             complement(11902..12576)
                     /gene="ciaR"
                     /locus_tag="VVL45_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HEU4186435.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(12685..15231)
                     /locus_tag="VVL45_000174"
     CDS             complement(12685..15231)
                     /locus_tag="VVL45_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEU4186436.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLAAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEASQVASQIFATHKENLAGLPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(15354..15794)
                     /locus_tag="VVL45_000175"
     CDS             complement(15354..15794)
                     /locus_tag="VVL45_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEU4186437.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDVILDSQNQAVCIVEITKVSVKPFNRVSADHAYKEGE
                     GNKTLVYWRQVHEDFFRDCLGEAGLTFTPESKVVLEEFRKVYPL"
     gene            complement(15769..16638)
                     /locus_tag="VVL45_000176"
     CDS             complement(15769..16638)
                     /locus_tag="VVL45_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HEU4186438.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNILLQS
                     CQEFVKDYLDTICSFYLGKEVWYRFTELTNTEANCLVGTKEFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSVIFPFVNDADQLKQAITVLRQQGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKIDFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(16616..17116)
                     /locus_tag="VVL45_000177"
     CDS             complement(16616..17116)
                     /locus_tag="VVL45_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU4186439.1"
                     /translation="MEIKNHFGVYCVCFENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELKNFMVHHVIALYDVEMNESAPQV
                     TISEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGFPELDKTSYMNWKVND
                     EKSTCP"
     gene            complement(17127..17825)
                     /locus_tag="VVL45_000178"
     CDS             complement(17127..17825)
                     /locus_tag="VVL45_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEU4186440.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     RFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(17818..18084)
                     /locus_tag="VVL45_000179"
     CDS             complement(17818..18084)
                     /locus_tag="VVL45_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEU4186441.1"
                     /translation="MKNVELKEKNMTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTI
                     EQNGLALEMTPYFLINVSGEGEGFSMWSPTVCDVLATDWVEVHD"
     gene            complement(18150..19076)
                     /locus_tag="VVL45_000180"
     CDS             complement(18150..19076)
                     /locus_tag="VVL45_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEU4186442.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFNLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCQLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(19151..19966)
                     /locus_tag="VVL45_000181"
     CDS             complement(19151..19966)
                     /locus_tag="VVL45_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4186443.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            complement(19970..20290)
                     /locus_tag="VVL45_000182"
     CDS             complement(19970..20290)
                     /locus_tag="VVL45_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU4186444.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
     gene            complement(20632..20712)
                     /locus_tag="VVL45_000183"
                     /pseudo
     CDS             complement(20632..20712)
                     /locus_tag="VVL45_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(20756..22753)
                     /gene="metG"
                     /locus_tag="VVL45_000184"
     CDS             complement(20756..22753)
                     /gene="metG"
                     /locus_tag="VVL45_000184"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEU4186445.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIIAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
     gene            complement(22877..24136)
                     /locus_tag="VVL45_000185"
     CDS             complement(22877..24136)
                     /locus_tag="VVL45_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4186446.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSSFTVSGKEEEIPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNIDEIASIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            complement(24138..24839)
                     /locus_tag="VVL45_000186"
     CDS             complement(24138..24839)
                     /locus_tag="VVL45_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4186447.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            complement(24823..26022)
                     /locus_tag="VVL45_000187"
     CDS             complement(24823..26022)
                     /locus_tag="VVL45_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU4186448.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTALKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVARADRHINELNQARNEAASAPAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            26287..27633
                     /gene="gor"
                     /locus_tag="VVL45_000188"
     CDS             26287..27633
                     /gene="gor"
                     /locus_tag="VVL45_000188"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEU4186449.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESIAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
     gene            27830..28366
                     /locus_tag="VVL45_000189"
     CDS             27830..28366
                     /locus_tag="VVL45_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEU4186450.1"
                     /translation="MKKAHVYAIPAIGAALIAALAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALAVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
     gene            28417..28503
                     /locus_tag="VVL45_000190"
                     /pseudo
     CDS             28417..28503
                     /locus_tag="VVL45_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(28572..28787)
                     /locus_tag="VVL45_000191"
     CDS             complement(28572..28787)
                     /locus_tag="VVL45_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEU4186451.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            28885..29205
                     /locus_tag="VVL45_000192"
     CDS             28885..29205
                     /locus_tag="VVL45_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4186452.1"
                     /translation="MRHKFQQVLNKIHDFLNGYDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFSKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            complement(29236..29574)
                     /locus_tag="VVL45_000193"
     CDS             complement(29236..29574)
                     /locus_tag="VVL45_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4186453.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(29586..30305)
                     /gene="trmD"
                     /locus_tag="VVL45_000194"
     CDS             complement(29586..30305)
                     /gene="trmD"
                     /locus_tag="VVL45_000194"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEU4186454.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            complement(30295..30813)
                     /gene="rimM"
                     /locus_tag="VVL45_000195"
     CDS             complement(30295..30813)
                     /gene="rimM"
                     /locus_tag="VVL45_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEU4186455.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELVLFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPSKRV
                     DVEILEGLDDED"
     gene            complement(30844..31632)
                     /locus_tag="VVL45_000196"
     CDS             complement(30844..31632)
                     /locus_tag="VVL45_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU4186456.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADAVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKRQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFEDVSIEAHIPVFVFCQP
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELLE"
     gene            complement(32209..32448)
                     /locus_tag="VVL45_000197"
     CDS             complement(32209..32448)
                     /locus_tag="VVL45_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEU4186457.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            complement(32468..32740)
                     /gene="rpsP"
                     /locus_tag="VVL45_000198"
     CDS             complement(32468..32740)
                     /gene="rpsP"
                     /locus_tag="VVL45_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEU4186458.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            32910..33119
                     /locus_tag="VVL45_000199"
     CDS             32910..33119
                     /locus_tag="VVL45_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4186459.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERELDTWLLSPKPVPKRNMDL
                     LVDDSLAGDIILLWRIQFGTFTTET"
     gene            33598..33811
                     /locus_tag="VVL45_000200"
                     /pseudo
     CDS             33598..33811
                     /locus_tag="VVL45_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            33895..34698
                     /locus_tag="VVL45_000201"
     CDS             33895..34698
                     /locus_tag="VVL45_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU4186460.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVIFHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(34754..36295)
                     /locus_tag="VVL45_000202"
     CDS             complement(34754..36295)
                     /locus_tag="VVL45_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU4186461.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMKMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(36297..36779)
                     /locus_tag="VVL45_000203"
     CDS             complement(36297..36779)
                     /locus_tag="VVL45_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU4186462.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVCFCFTPQEQSTVGVGTPGIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKVRN
                     "
     gene            complement(36779..37864)
                     /gene="rlmN"
                     /locus_tag="VVL45_000204"
     CDS             complement(36779..37864)
                     /gene="rlmN"
                     /locus_tag="VVL45_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEU4186463.1"
                     /translation="MKPSIHSLVHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMRQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFDERGQDERISHIVVM
                     GIGEPFDNYNNVLNFFRTINDDKGMAIGARHITVSTSGLAHKIRDFADEGVQVNLAVS
                     LHAPNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALE
                     LTELLKNIKKLSYVNLIPYNPVSEHDQYSRSPKECVLAFYDTLKKKGVNCVVRQEHGT
                     DIDAACGQLRSNTMKRDRQKAVAAVNP"
     gene            complement(37885..38397)
                     /locus_tag="VVL45_000205"
     CDS             complement(37885..38397)
                     /locus_tag="VVL45_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEU4186464.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDDK
                     GQRHFVIRQK"
     gene            complement(38553..39158)
                     /locus_tag="VVL45_000206"
     CDS             complement(38553..39158)
                     /locus_tag="VVL45_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEU4186465.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            complement(39331..40368)
                     /locus_tag="VVL45_000207"
     CDS             complement(39331..40368)
                     /locus_tag="VVL45_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU4186466.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQMLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKAFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKANSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            complement(40402..41337)
                     /locus_tag="VVL45_000208"
     CDS             complement(40402..41337)
                     /locus_tag="VVL45_000208"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEU4186467.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            complement(41890..43080)
                     /locus_tag="VVL45_000209"
     CDS             complement(41890..43080)
                     /locus_tag="VVL45_000209"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEU4186468.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAADLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            complement(43295..44638)
                     /locus_tag="VVL45_000210"
     CDS             complement(43295..44638)
                     /locus_tag="VVL45_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU4186469.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLASNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            complement(44778..45593)
                     /locus_tag="VVL45_000211"
     CDS             complement(44778..45593)
                     /locus_tag="VVL45_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU4186470.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLSEQGKNYYWTWAYNHIGYNRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAILNWK"
     gene            complement(45645..47825)
                     /locus_tag="VVL45_000212"
     CDS             complement(45645..47825)
                     /locus_tag="VVL45_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU4186471.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFAPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKDLTEEVYSVADGQVVALEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            complement(48111..49037)
                     /locus_tag="VVL45_000213"
     CDS             complement(48111..49037)
                     /locus_tag="VVL45_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4186472.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKI"
     gene            complement(49030..49722)
                     /gene="ftsE"
                     /locus_tag="VVL45_000214"
     CDS             complement(49030..49722)
                     /gene="ftsE"
                     /locus_tag="VVL45_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEU4186473.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            complement(49740..50835)
                     /gene="prfB"
                     /locus_tag="VVL45_000215"
     CDS             complement(join(49740..50762,50764..50835))
                     /gene="prfB"
                     /locus_tag="VVL45_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEU4186474.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQDKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            complement(50943..51599)
                     /locus_tag="VVL45_000216"
     CDS             complement(50943..51599)
                     /locus_tag="VVL45_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU4186475.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKISILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            complement(51907..52617)
                     /locus_tag="VVL45_000217"
     CDS             complement(51907..52617)
                     /locus_tag="VVL45_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4186476.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVASGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGRIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            complement(52617..53381)
                     /locus_tag="VVL45_000218"
     CDS             complement(52617..53381)
                     /locus_tag="VVL45_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4186477.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            complement(53381..54337)
                     /locus_tag="VVL45_000219"
     CDS             complement(53381..54337)
                     /locus_tag="VVL45_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU4186478.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKSPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            complement(54341..55210)
                     /locus_tag="VVL45_000220"
     CDS             complement(54341..55210)
                     /locus_tag="VVL45_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU4186479.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            complement(55478..56638)
                     /locus_tag="VVL45_000221"
     CDS             complement(55478..56638)
                     /locus_tag="VVL45_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU4186480.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKNNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            complement(56739..56987)
                     /locus_tag="VVL45_000222"
     CDS             complement(56739..56987)
                     /locus_tag="VVL45_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEU4186481.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            complement(57066..57656)
                     /gene="clpP"
                     /locus_tag="VVL45_000223"
     CDS             complement(57066..57656)
                     /gene="clpP"
                     /locus_tag="VVL45_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HEU4186482.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            complement(57830..58459)
                     /gene="upp"
                     /locus_tag="VVL45_000224"
     CDS             complement(57830..58459)
                     /gene="upp"
                     /locus_tag="VVL45_000224"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEU4186483.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLNLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(58548..59015)
                     /locus_tag="VVL45_000225"
     CDS             complement(58548..59015)
                     /locus_tag="VVL45_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEU4186484.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            complement(59034..59591)
                     /locus_tag="VVL45_000226"
     CDS             complement(59034..59591)
                     /locus_tag="VVL45_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU4186485.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            59716..60561
                     /locus_tag="VVL45_000227"
     CDS             59716..60561
                     /locus_tag="VVL45_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU4186486.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            60620..61795
                     /locus_tag="VVL45_000228"
     CDS             60620..61795
                     /locus_tag="VVL45_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU4186487.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            61815..62270
                     /locus_tag="VVL45_000229"
     CDS             61815..62270
                     /locus_tag="VVL45_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU4186488.1"
                     /translation="MNRREAVEFVNMCMIKNGDKVLVQDRVNPDWSGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEDLSEFFYYKNGDDWLYDLK"
     gene            complement(62299..63039)
                     /locus_tag="VVL45_000230"
     CDS             complement(62299..63039)
                     /locus_tag="VVL45_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU4186489.1"
                     /translation="MYHIKEAAQLSGVSVKTLHHYDKIGLLVPLKSENGYRTYSQEDL
                     ERLQVILYYKYLGFSLEKIAELLKEERTDLLPHLTRQLDYLTRERQHLDTLISTLQKT
                     IQEQKGERKMTIEEKFTGFSYQDNQKYHQEAVEKYGQEVMGQALERQKGHEDEATAAF
                     NQVFQTLAQNLQVGLPATATENQEQAAKLLQAIRTYGFDCSIEVFGHIGKGYVYNPEF
                     KENIDKFGSETAQYTSDAIAVYVQTNAE"
     gene            63267..64614
                     /locus_tag="VVL45_000231"
                     /pseudo
     CDS             63267..64614
                     /locus_tag="VVL45_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466604.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            complement(64654..65598)
                     /gene="manA"
                     /locus_tag="VVL45_000232"
     CDS             complement(64654..65598)
                     /gene="manA"
                     /locus_tag="VVL45_000232"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEU4186490.1"
                     /translation="MSEPLFLQSVMQEKIWGGAKLRDEFGYDIPSEKIGEYWAISAHP
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDDLLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTVKADDLRSTLLVSNDFFAVYKWEITGKVDFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGSHFILPSDVEAWTLEGQGLELIVSHP"
     gene            complement(65719..66015)
                     /locus_tag="VVL45_000233"
     CDS             complement(65719..66015)
                     /locus_tag="VVL45_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEU4186491.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYQDGVDLLGYTTWG
                     CIDPVSAGTGEMNKRYGFIYVDRDNVGNGALKRSKKKSFYWYKDVIYSNGASIG"
BASE COUNT        19391 a        14445 c        12091 g        20158 t
ORIGIN      
        1 gttaccaagt tactcaaagt acagatttga agctgcagat aagactgacg aaatcacttt
       61 aatactaaat gaaaatcaaa aagcaaacta agagcctcag gctgtacttg agtacggtaa
      121 ggcgacgctg acgtgatttg aagagatttt cgaagagtat aaaacactgt tttgaggtgg
      181 tggataaatc ggctgggcaa aaactcaatc tcaggagaaa atgaagtaaa tctccccaca
      241 actatagaaa aagatttgat tctgctcctc acacaataaa atctttttct tttattaaaa
      301 acgaatcgtt tcacgtgttt tctcatatga ggtaacaaag cgattggtta caccaggctc
      361 tgccacttcc aatgcttgag aaatcttgtc aaatgaagcg tgataatcaa attctttttc
      421 aaaaatatct aaagcttcgt taaatgcttc ttgaatgcgt tcatcaaatg agcgatagcg
      481 gttagaatat tgcaagagtt gctctgtcaa agttgcatat tgtacaatat tataagtttc
      541 cgtttctaaa gcttccatat cattcgttgc aatttcaaga actcgggtaa cagattcaat
      601 gttaatcatt ttttgttcta actcaaccat taaatcctcg gtattattgc ttgccgtaaa
      661 gaataacttc aagaaagttt gtggaatacc tggcagattg cgtttttcca tgtatcgctt
      721 gatagtatgg agacgattga cataaacatt ggccttttga cgtgcattaa tatcatcttt
      781 ctcaatttgt gtcaggcgct cactaactga aatttgctca tcttcaatat cttttagttg
      841 agtttgtaaa tcctcaagat tttcttcaag aactgaataa gcttgggttg gttcttcttg
      901 atttgaagtt acctcaacaa tagctgcctc aaaactctct aattctgtct gaatacgacg
      961 aacatggctt gcagctgtct caggaagtaa ataggtcttg ttcaaacgtg caatatcttc
     1021 tcccaataaa gtattattct ctttcatatg ttgaaggtaa gttggaagag ttgcaagtag
     1081 attttccact actttctgag cagcaatttc tcgagtaaaa atatcataca aggcattgat
     1141 ttcctcttgt gcctgtccat tctcatattc ggcattatcc aattccaact gacgaatatt
     1201 ctcttggttt ttcttgaatg cttcataaag caagtggaaa cgcgcttcaa tatccgtttc
     1261 aacaaaatga taattagcat caattagttt acgataaccg gcttccaaat cctgtaattg
     1321 atctggcaat tctgtagaaa gcgtcgtaac caaggctgga acacgatcca caatatgact
     1381 taaggccaaa atgtgatttt ctgtattatc caaaatcact gcggcttcca cagggtcacc
     1441 cgatgaattc aaggttacaa attgtgaaaa ttcagattgg atattttcta attgtttttc
     1501 aatttcatcc aaggcttgac catactgttc tgaattttca gcaactctat gctgaagttc
     1561 ctcaaataaa tccaaagcat gaagaacacg accactattt ttagattctt gcttctctaa
     1621 gtctgccaaa gcattgcgaa ttgccgcaat atcttcttca atcaaagtaa tttgactctc
     1681 aatttggtca atttgatgac tggccttgag aaaacgaaat gaatggttat aaccttctgc
     1741 ttcaaagaga ttattttcaa tatcggcaaa agagttgaga gataaatcga cccatttttg
     1801 attccattca cggaaagcca cttgactttg tccaatcaag tgcatatttt ttacagcttc
     1861 tacttcatca tttactggaa gattgtatag ttcttctttt ctttcttcta gtgcctctaa
     1921 tctcccctcg tttcgcttac gtagaaagat tgccactaca taagccagaa ctaaaatgac
     1981 tgcaattgca accattaaat aaattagttg tccattagac atatcaaact ccttttttac
     2041 ttgaaacaat cgtaacgatt atatcatatt ttttagacca agggaactac ttctccctat
     2101 tttttagaca tcaagtgtac tatagacagc attttcttcg ataaactcac gacgaggctc
     2161 tactcgatcc cccatcaaca tatcaaagat tttatctgct tctgcagcat catctacaga
     2221 aactctagcc atcaagcgat gttcgggatc catggttgtt tcccacagct gatgatcgtc
     2281 catttcacct agccccttat aacgctgaat agtcggtttg gtacgacctt cactataacg
     2341 ggctaaagct tcttggagtt tgatttcttg atctgcaccc ggctggatat attctttaat
     2401 ctcgcttcca accttgacac catagattgg tggttgggca atataaacat aaccagcttc
     2461 taggattggt ttcatataac gataaatcaa ggttaaaaga agggtacgaa tatgggctcc
     2521 atcgacatcg gcatcggtca tcaaaacgag tttttggtaa cgggctttcg aaacatcaaa
     2581 ttctgcgcca aatcctgttc ccatggctgt gaaaagacta cgaatttctt cgttagctag
     2641 aatcttatcc atacttgctt tttcaacgtt caaaatctta ccgcgaattg gaaggatagc
     2701 ctgaaactca cggttacgac cagatttggc tgatccacca gctgagtctc cttcgacgat
     2761 gaagagttct gtttcagcag ggttattaga agaacagtct gctagtttcc ctggaaggtt
     2821 ggaaatttcc aaaccagatt ttttacgtgt gacttcacgc gcacgcttgg cagccacacg
     2881 agccttggca gctaaaatcc ctttttccac gatacgtttg gcaatctgtg gattttccat
     2941 gaggaaatcg gagaaggctt cactgaagag gcgattggta atcttgacca cttcgctatt
     3001 tcccaatttg gtcttggttt gtccttcaaa ctgtggattt gggtgtttaa ctgagataac
     3061 tgcagttaat ccttcgcgaa catcttcccc tgttaaatta tcttcattgt ctttcagtaa
     3121 cttattctta cgagcataat cattaataac acgagtcagt gccgtacgga aaccttgttc
     3181 atgcgttcca ccttcatggg tatgaatatt attggcgaaa ctcatgacat tttcatggta
     3241 acctgttgtg tactgcatgg ctacctcaac tgtaatatca tccatctcac cgtctgtata
     3301 gattggtgta tcaaagatta catccttgtt ctcgttgata tattcaacgt aactagcaat
     3361 cccaccttca taatgataat gcttggtttg ttccaaacct tggcgcttat ctgtaattga
     3421 aatttgaaga ccgcgattta gaaaggccaa ctcttgaatc cgtttattta atttatcaaa
     3481 atcaaagatt gttgtttcag tgaagatttt tgggtccggt gtgaagtgaa cagttgttcc
     3541 tgttttatcc gtatctccaa ctatttcaag atctgcgaca acatgaccac gacggtattc
     3601 ttggtaatga atcttaccat ttttgtgaac atgaacgtct aattgagtgg aaagggcatt
     3661 aactactgac gaccccaccc cgtgaagacc acctgaaacc ttgtatccac caccgccgaa
     3721 ctttcctcca gcgtgaagga ctgtaaagac ggtctcaaca gcaggacggc ctgttttttc
     3781 ctgaatatcg actgggatac cacgcccatc atccacaaca gtaatcgaat catctggctc
     3841 aataaaaact tgaatatggc tggcaaatcc tgccaaggcc tcgtcaattg agttatcaac
     3901 aatttcccag actagatggt gaagaccttc ttttgaggtt gatccaatgt acatccctgg
     3961 acgcatacga acagcctcta agccctctaa aacttgaatt tgactggcat cataatcctg
     4021 tgcctgcaga tttttgattt cttctgtcat cttattcctt tttcttatat gtctaatact
     4081 tgtcttaaat tcacgatact ggtaaacaag tgggtatcaa gtcctgcagc ttgacctgct
     4141 tcgatatcaa tcggccgatc accaatgaca agaccagagc taatctgata cttttctctt
     4201 aaataaagca tggattcggg atttggcttt ctcttaaagc ctgagctaga agtcaccact
     4261 tctgtaaaat aagctgctat agaggttttt tctaaaattt ccaaaacctg atcatttcga
     4321 tgagagacca aaaaatgacg gccaccttga tttgaaatgt cttccaatag gtcagaaact
     4381 ccttcaaata aaatcgggtg ttcaagctct ctggcttcat tttccttgta cttttctaaa
     4441 aaattctcta aattgggagc gaatgtctca atcgcaaaag gagtagaaac ctttaaagct
     4501 tgatagacac tgtcatggtc ttgtgtgata ccatacagtg ccaatgtttc aacaaatgca
     4561 gctgttgaag tttcataatt atccagtaaa gttccaccta aatcccagat gtaatcgtga
     4621 tatttcatac ccttcattat agcatttttt tatgaaaaaa gcgatgattt ccctgagttt
     4681 tccacataaa aaacgagaga ataactcccg ttttactgat ttttaccaaa gacatctcga
     4741 tagagcattc ctgttcgtaa actctctgcg tcccctccta gttgctccac caactcgtag
     4801 gcaaaggcaa gggctgttga aggaccccga ctggttgtca actgaccatc taccactact
     4861 gtttccttga cgtagtgacc atcaaggatt tgctcttgaa cgccgtcata acaagtgtat
     4921 cgcttatttt tcaatatctc tgcttgattg agggcaattg gtgccgcaca aatggctgct
     4981 agtttcttcc cttcttgctc gaagctttgc aattcttgaa tcaaggtctg attatcacgt
     5041 aaatgtgcag aaccaggcat acctccagga agaacaatca tatcatagtc tgataaatct
     5101 ccatcaaaga catgatctgc tcttacttgg attgcatgcg aacccgttac ttgctcttca
     5161 aaaccaacca tatcacatgt gatattggct cgacgcaaga catctacaac tgtcaaggct
     5221 tcaatttctt caaagccctg agctaatata actgctactt ttaccatgat tttctcctta
     5281 tttaatttgt tttaatacaa cctttttccg tttgaatggg acttttccgc tcttttcttc
     5341 agctagatta gtctggtaac tcatcgataa aagcaaacca acaccaatca gattactgat
     5401 aatagctgat cccccttgcg aaatgaaagg caagggaatc cccgtcaaag gaagtagtcc
     5461 agtcacagca ccgatattct caaagatgtg gaagagcaac atcataatca aacctgtgga
     5521 aatataagtg tagaactggt tatttgattt aagagtaatc ttcaacatac ggtaaatcaa
     5581 catgagatag agggcaataa ccaggacaga gccaataaag ccaaaatctt ctgcaataac
     5641 cgtaaaaatc atatctgact ctcgaactgg gataagcaga ttcgaagcat taaatccctg
     5701 accaaataag ccaccactcc caatggcaat ctgcccttga gcctgctggt aagtcgttgt
     5761 ttgggcaaac tcaaagggat tgagccaagc caaaatccga ttaatttggt aggtcggcat
     5821 tccaatctgg tgaagaaaag ctcgtccgtc cttgctaata aagatagcta agaaaccagc
     5881 aactcctgtt acagcagtca caaatactgg gataataatt ttccaagaaa cccctgataa
     5941 taaaacgatt cctgagaaaa tggctacaaa aaccaaagcc gtccccaagt cactttgaag
     6001 tgctaaaaga actaggactg gaatggtaaa gagaatcatc cagaaaatta acaaaaagtc
     6061 cagcggaacc gtgcgtctcc attccttatg tttctttgta aattggacaa tgacacgagc
     6121 caacatgagg atataggata tcttcataaa ttctgacggt tggaataggg taattccatt
     6181 tattgatacc cagtttttgg cacccgttga tgcaactaag cttggattat aaaatacaat
     6241 cggcaagatc ataagtccca agcctaaaat atatagaaag ggggtcacct tccaaagaaa
     6301 ttctgtatta aagagcatga ccacaaaacc aatcacaagc cccaaggcaa tccaggcgac
     6361 ctgctgccct aaaatgggca gaatattatt gggataatca tgactaacgg ctatatagat
     6421 agccaccaca ccgatgacca gtagaaaaaa tactggcaag agcaaactgt aatcgactct
     6481 tgagtcgaga gaacgtttca tatatactaa ccttatactt tcatacaata ctatttatca
     6541 aagctcatta aaaaatctat caatagcctc gtcaacttcg gatcgagaga tggttttaac
     6601 agtcgcttct tctgctagag atgctactat ttgtttgccg tatcgttttc cgacgattct
     6661 cctatccaaa ataagagtta aggaacgttg gtattcacgt ctcatacttc ttcccaaagc
     6721 ctgttttaaa cgaataatgg ccattggcaa ttgataatca taaaaggcat ttttcccttc
     6781 ttgattcagt tcttgattaa tctttttcgt caagggttct tgaggatttt ggaaaggaag
     6841 cctcggtaca acttgaatca cagaaggatg gcttgaaaaa tcaactccct cccagaaact
     6901 tgctgcacca agcaagattt gttgttcacc tttttcaaag cgtttcttta gctgatgaac
     6961 atccccattt ttatactggg ccaagtggct aactgtaagt aaatccgata ctgctagaag
     7021 catgtcttta gcggtaaaga gaaccaaaat cggttgctgg aaagcttgaa tttccactag
     7081 taaagcagct acctctctgg catagacttc taaggaggtt tctgttacca gagggaaatc
     7141 tttgaccatg accacttctt gttcctgttt tccccgagat tcaatcttga caaatttagc
     7201 ttcaggatag cctaaaaggt ctgccaaaga aaccctctga ctaatctcaa gagtagccga
     7261 tactcccaag acttggcaac tctcaggaag taaagaggac agaagagtac ggcctgattt
     7321 tgtagaagaa atctgaattt tcttttgact cgtttcagtt acttcaagcc agtaatcacc
     7381 ttcagctgta aaatagcgaa ccagctcatc aaagtcttct acttccaatt ctgaaaaata
     7441 ctgatggaga ttgtccagag aatctaagat atttttccta gatttgccag actgaaattg
     7501 ttctatcaag tagagacact caaagcgaat actttctagt atccgttgtt gaaccctgtt
     7561 ttcttctcct actaaagcct tatcaattaa atcgataata gattgtatat cgtaggtctc
     7621 ttgaagcaga ttttctagag ctaacaaaat cttttggact tcatcaataa tcagtaaacg
     7681 gtcactgaca aattcaggat tatcttcaag tctggttacg agataggcat gattagtcac
     7741 taaaagcttg caagtctctg ccctttcttg actacgtttc caaaaatctt ccgtcacaaa
     7801 taagctctgg gatgataaat tcccatcatg acgaaggtct gctagaaaat gttggtaacg
     7861 gtagagttgc ccgatttcat ccaaatctcc tgtctctgtc tcagtaagcc agaccaagac
     7921 ttgcatttta aagcgtctaa ataagcgatt ttcatcattt tcctgcaagg aatgataaaa
     7981 ggcatccaac ttcagataat tttgtggtcc ctttaagcta tgaatatctg tatggaacac
     8041 ttccttgagg cgtttacctt cttcttccat gatttgattt tgaagaatct ttgtcggaac
     8101 actaagaaca atttgtcgct ctttggattg agataaagcg ggtaagagat agccataggt
     8161 tttcccaatc cctgtcggcg cttgaatcag agagacaggt tcatctttca atagcaagcc
     8221 aacctcttta gcaaaacttt cttgttcctc cctcacttca aggttcaaca gagaaatatt
     8281 tttagaaaag tcttgagata gttttcgtgg ctccagagaa gcttccgttt tcttaaaata
     8341 tagaccttga acttggacca agtctggaga actcaggata gattggttgc gataagtttc
     8401 ctcaataacc aggtaggact catataggag agcgtcagcc atttccagca agcgttccaa
     8461 gagaccttta ggaagctggg tcatcttttt ccgtaaaaaa agaagtaatt ctgctgtagc
     8521 ttgggcatct gaaagggctg tgtgtgcgtg tttaagagga attcctaatt ctcgacacaa
     8581 aatcggcaag ctatattttt ccagttcagg gaaaaagacc tgggccaatt cgaccgtatc
     8641 aacacgaggg tttcttagct catagccttc aaaaaataaa ttttccgcca agagattagc
     8701 atcaaactga acattatggg ctacaaaaat cccatcctcc accaagtcaa atatttttct
     8761 ggcaacttgc gaaaaatcag gtgcttgcgc cagacgttgg tctgtcaatc ctgtcagttc
     8821 tttgatatga gcatccaagg gttcatgtgg attgacatcc gtcgtatagt gatcgacgat
     8881 ttctccgtcc tcaatcacga caattcccac ttggataatt ttagccttac tacctgtgct
     8941 agttgcctct aaatccacca caacatagcg attaccaatc gttttcatta taaaaaatta
     9001 taccaaaaaa agggccattt ggcacctgca aacataggat aattttcaaa ctcaagaaca
     9061 ctacttttct taaaaatccc atcgaataga gcattctagc ctgctgattt ttgtaaaata
     9121 gtgaagaaat acgaaaaatt gagagaagaa atgtttgcat cttccttctc tcaacgaatc
     9181 atgtatttta tctaaccatg tcaggatcgt agtcataaaa tcctgtatca aggaaacggt
     9241 ttttagggtg aagtttacga acttcttcat ccagcaagaa ggcttgagca tgactaaagt
     9301 tgccctcttg aatcaagcta cgcgcttgga taaagagttt tgcaatctca acaaagttct
     9361 gaccaggagt gtagagccct tctagtttcc agcgagtaaa gccatgctcc accaattctg
     9421 ttaatttgat catcaaatca aggtcattgt tggcaaagat atgggtccca tgattatctt
     9481 caaaaatgga atagtggctc tctggatcac ttggctcagc caagaagagg tcacgtttat
     9541 gcgtcttttc atcatcgata tgtgtaaagt tatagtagtt ttgcaagagt ggacgtttag
     9601 aatgatggat gacgctagca ccgtaaacca aaacttcagc aggaatttcc aaaatctctg
     9661 gcattttgaa aagttcagct gatggaattt cacgcgccaa aacagcctca gatgcgccag
     9721 ccttttgtcc ccagaagtta atctgacgac tgctagttac catggttgaa gcatcgtaga
     9781 tggtcttaaa tgaataacca tcgcggttaa ctacgtaaaa gacgcctgca tccccaatcg
     9841 taatatagtc tgtcttgatt tcttccaaga agtttaagaa aggcttgata cggtccatca
     9901 tatcttggtg catgagagca ttgaccgcaa cgatcaattc cttaccagca tcatgaacca
     9961 acttagcgat ttcacgtaat tggtcataac taaaggtcgt tggcagacga agaccaaaat
    10021 ctttctcacc gacatagata cggtctacgc cagcttcgag tagttgttca acttgttcaa
    10081 tactttcagc agttgctgta atgacaatct tttccataag aaaaattata ccacatttct
    10141 ccaaaatcag ccatgctttg aaaatgaaaa gggactttat tctttttgta acctttctgt
    10201 aataattctc tgccgaaaaa atgataaaat aggtatgtac tgttaaggag agaatcatgc
    10261 ccgtaagaaa attacaatcc tatgaggtag actatcaaga agaattaaac cagcagcttc
    10321 ctcattacca agcttataca cctgaagcgc aatctgatgc caatctcaag gaaatcctat
    10381 tttttattaa tatcgctgtt ttttgtatct gtattgctat ctttagtttt atctttttag
    10441 cattaaaatt atcaactgct cttgcctttg ccgcagcaat cggattcagc ttacttgttt
    10501 taaaagttca acggtctatt atcaaacgaa aacgtagaag ataaatccaa aatatcgccc
    10561 caattggagc gatattttta ttttttcttt ttagatggtg tctgaatggc aatcttcact
    10621 tcaaagattg ttcccttggg tttattatct ttgacagtaa cagttccttt tagagcatct
    10681 acaatttgct tggctaggga taatcctaaa ccaaaaccac ctttttgccg ggttctagcc
    10741 ttgtctactc gataaaaacg gtcaaaaatt ttctttttat cttctgtcga aataccgatt
    10801 ccattatcag aaacaagtaa ataaagattg cgatcggtcg ccgagataag aaaatcaatt
    10861 tcaccatcct cctcagtata cttgacggca ttatcgaaaa gaatggtcat cagttgtttc
    10921 agaagaagct gatctgtgac aattgttcga tggatacgat tttcaaaacg gaagacacga
    10981 ttattttccg aagcaatcat ctcgtagttt gtgaaagttg tattaaaaaa gctagttgga
    11041 acttctgcaa gctccggctt aatcccatca tctctccgag ctaagttcag caagcttgtc
    11101 gttaaaaaac gcatatttcg gacttcttcc aaactcgatg caatgctttc gctcacatcc
    11161 ataatggtag cttctggctt acgaaaaagg gtctctaagc gattttgcaa aactgcgagt
    11221 ggagttcgta actcatgact ggcattttcc acaaaagact gttgcttctg catactctca
    11281 agcaggggcc taacactgac cctagctaga tagagactgg caagtaaaga caaaatccag
    11341 aaactagcca tcacgaccac aatcaattgc tcatgctttt gactagcctg ttccaactga
    11401 ctggtattaa tcaagacagc agcatacttg atattggttg aaaccgaact aatattggtc
    11461 tccatcaaaa tcacacgata gatttcttcc tgcccatagc tattaaaaac ctgaatctgg
    11521 tagatatgtc ctagttcttt cttttctaac ttaatcttat ccaagcccaa aaatcgattt
    11581 ccagaaagaa gttgagtaaa gtctttatca aagagaatga cttccgtatt ggaactgaca
    11641 ttaggtttta tttctacttt actagcgtcc gccctagcat tttctaaatc tttaatctct
    11701 tctgttgccc tatttatagc cagctgaata actgcttgag gattttcact cagtccatga
    11761 agcttatcgt ccaccgaagt ataaagactc gaatgcatga cttgtaaaat aatcagagtc
    11821 attgtagaaa aaatcagggt gaagacaccg aagttgcgga taaaataact aaagtcatcc
    11881 gcataccatg tttttttaag tttactgaac atcttttaaa agatacccaa cactacgcaa
    11941 agtttgcaaa ttctctgcaa aagtggttcc ctttaatttc ttacggactt ttgaaacata
    12001 gacttcgaca accgaaatcg ttgtatcact atcaaatccc catagacggt caaaaatctg
    12061 cgtcttaggc aaaatcacat tttgattttg aaggaaataa actagtaaat cgaactcttt
    12121 ccccagcaat tcgacaggag tatcttcaac tttaacggta ttggttgata aattaaccac
    12181 gatattccca taagtcaagg tgttttcatt aaacttccct gaacgtttga gaagggcctg
    12241 aatccgcatt ttaagttctt ctaggtagaa aggtttggtc agataatcat ccgctcccag
    12301 ttcaaatcca tgtcccttgt catccaaact ttccttggca gtcataatca gaactggtgt
    12361 cgtaattccc ttttcacgca attcttttaa gacttggaaa ccattttttt ctggcaacat
    12421 caaatccagc aaaatcaagt catagacacc actctcagct tcgtagagac cttcttctcc
    12481 atcaaatacc tgcataacat ccgcaaaatc gtctaaaaag tcaaatactg aatttgacag
    12541 acctaggtca tcctcaacca ataagatttt tatcatgaga aactcctcct tattaaaact
    12601 attataccaa atttgcctta aaaaaaactc aactctctgc attttacatg agatggctga
    12661 gttttctttt tattttaggc ttatttatgc atttccgtat tgaagaacaa ctgcttcgac
    12721 tgcagctttt tcacggctaa tcaagtcaac acgcgctgca atttccttga ttcccatacc
    12781 gatgttacgg ctaagagcaa ggtcagaaag ttgcggttca aagaactcct tgtattccgc
    12841 caagcgttgc tgagtcttaa atacatgagc aggaaggata acaaagctat caaagctcat
    12901 atctcctcca agggctgcct taatccaagc ccagttttca cgcgcccaag accaagctgt
    12961 tttctgagtt gcttgatgag ctaggaattg gtaataccaa gcagacaagt cctgtggttt
    13021 gaccacaaat ttgtccttcc aagaagtaat caggttttgg atattatccg catctgtact
    13081 gtatgcaaga gctgctgcca actggcgttt aaagacagca tctgttgcgt gagtataagt
    13141 atcaagataa agtgctaaca agtctttagt ctcatgatgt ttcatctcat taatcagaac
    13201 ttgtgagcga atagctgctg ggagtcctgc aagattctcc ttgtgtgttg cgaagatttg
    13261 gctagcgact tgactagctt ctgcatcatt tgagcgaatc atcatcgaaa cagccagctg
    13321 acgaaccaat tcatcctcat ctgattctcc gtctttagct tcaaaaccaa gacggtcata
    13381 gttatgacga gccaatttag caaccagtcc tttgaaggct gtttcagcat ccgttccttc
    13441 atcaataaag cgctcaaggg ctgaaatcac ttgagaaaca gctgaaacca ccagataaga
    13501 ctcttcctta gcaagtttat caagaactgg aagcaagtct gcataagaaa tgtgccctgc
    13561 ctcagccaac aaacgacgtt cttgaacaat ttgcagtttg cttgtgttat caagtgtctc
    13621 tagctcagca agaacagctg ctaacaagtc tccttgatag tcggtaatat agtgggcagt
    13681 attttcagtg ttgagacgaa gagctccttc attttcagca agaagagctg cgtagccagg
    13741 gatttcgata ctttcagttt cgagtgtatc aggcaagcct ttccagttgc tattgagggg
    13801 caccacccag agacggttct tgtcttcgtt ctcaccgatg aagaattgtt tttgtgaaat
    13861 cttcaagaca tcattttcaa ctttaacagt aagaactggg taaccaggct gttccaacca
    13921 agaatccatg aaggctgcga catcacgtcc tgacgcttga ccaagggcat cccaaaggtc
    13981 actaccaatg gtgttgctgt attggtgttt ttcaaagtag gcgtgcaaac ctttagcaaa
    14041 atcagcatct cctagccaac ggcgaagcat gtgcatgaga cggcttcctt tggcatagac
    14101 gatagcgccg tcaaagagtg tattgatttc atctggatgt ttaacttcga cgtggacaga
    14161 ctgaacgcca tcagtagcgt cacgttcaag agcaagaggt actccacctg tttggaaatc
    14221 ttcaaagata ttccagcttg gttcgatggt atccacacag acgtattcca tcatattagc
    14281 gaaactttca ttgagccaaa ggtcatccca ccatttcata gtcacgaggt tcccaaacca
    14341 ttggtgagcc aattcatggg ccacaacaag ggcaacttgt tgacggctag caaatgtaga
    14401 gttctcatcg acaaccaagt aaacttcacg gtaggtcaca agaccccagt tttccatagc
    14461 accagctgag aagtcaggaa gggcgatgtg gagagattga ggaattgggt acttaactcc
    14521 atagtaatct tcgtaaaact cgatagagcg aacagcgata tccagtgaga aatcaagatt
    14581 tgaaagtgga tgtgctttgg ttgagtagac acctaccagg gtaccatttt tagttttagc
    14641 ggtcacccct tgcaaatcac cagcaacaaa ggccaacaag taagaagaca tgcgaggtgt
    14701 tgtctcaaac ttccagatac ctgtttcctt acggttttca acatcgattt ctggcatgtt
    14761 tgacaaggcc aattcacctt ctgcttggtc aaagcgaaga gagaggtcaa aagttgcttt
    14821 ggcttcaggc tcatccacac atgggaaagc ttcgcgcgca aaatggctct cgaactgagt
    14881 agacaagacc tccttcttga ctccatcaac tgtataataa gaagggtaaa tccctgtcat
    14941 gttgtctgta attttaccag aaaaggcaag aaccaattca acttgaccag cctcagccaa
    15001 ttcgatatga agggcttcat tgtcatggtc aactgtaaat ggacgagctt gacctgcaac
    15061 ttctacagag gtgatttcca aatctttttg gtggagggag atgcggtcac tctgtgcttg
    15121 accagtgatg gtcactttcc cagaaaaagt cttggtctca cgactcaaat ctaaaaataa
    15181 atcataatgt tcaggaacaa attgcttaat aaaatgttca actgcttgca tagttttctc
    15241 ctattctaag tttaagagct gctgctcttc ttcaaacaaa cttattatat catattttgc
    15301 ttcagaaaaa aggattggac agcgatttcc aaaactttct tccttctaat agtctacagt
    15361 gggtagacct tgcgaaattc ttctaaaaca accttgcttt caggtgtaaa agtcaatcct
    15421 gcttcaccca agcagtctct gaaaaaatcc tcatgaacct ggcgccaata gacaagtgtc
    15481 ttgtttccct caccttcctt ataggcatgg tcagctgaca ctcgattgaa gggtttaaca
    15541 gaaacctttg taatttcgac aatgcagaca gcctgatttt gactatctaa aatgacatcg
    15601 aaggtcccta cttggggaag tggttcgtct tctagcacat agaggtcata ggctgatgct
    15661 gttgctgtct tttctccttt aaacaccaaa tccgctaaaa ggtctggttc aactccaaaa
    15721 gcccaggcat cgatttcatc tccgatcaaa ggattgattt gcttgtattt attccacatt
    15781 tcttgcggta tcatgggtgc tcctttgtaa ttttttactt tcttctttta tgtgtttaag
    15841 atgatctgga tggtcaatct ctaaatcaaa aatctctgga atagaactgt agtggataat
    15901 gcacttgata cccaactgat tcattttttg tatgaaagaa gtattcagat agcctgctac
    15961 agcaaaatca atcttgttct ttcttgcttt atcctgcata tctcttagca tatctaacat
    16021 tattggactt tccatatcat gccattgact gtttctcata gtcgcaaaaa caaaggaagt
    16081 caaatcattc attccaacta caatctttga aatgcccgtt tccagtatac tagataagtc
    16141 aaaatacgct gacggtaatt caatcatcgt tccgactttc ccagtaaaac cctgctgacg
    16201 caatactgta atagcttgtt ttaattggtc ggcatcattg acaaaaggaa agataacaga
    16261 tagattgggg ttggtttgat aaacttctgt aacgacatgt gcttcagcct gaaattcatc
    16321 caaacacgcc agtaaacgcc tagttcctct atagccaaac aagggatgcc cttcgtcaaa
    16381 aaactcttta gtccccacta aacaattggc ttctgtattc gttaattcag taaaacgata
    16441 ccaaacttcc ttacctaagt aaaaggagca aatagtatca agataatctt tcacaaattc
    16501 ctgacaactt tgtaatagta tattttgatt gagctctctc aataagtatt ccccacgaat
    16561 catgccgacg tggtgaaata gttgaggata aattttttca agaatttttt cgccactaag
    16621 ggcaagttga tttctcatca ttcaccttcc aattcatgta agaagtcttg tccagttctg
    16681 gaaatcctaa taattcagac ttaaccttca agactaatgg cgatgcattt tcttctgtaa
    16741 tctcttgaat atccatccaa atatatccaa gtgaatcatt cgcaccatca gacacagctt
    16801 ccgaaatcgt aacttgaggt gcactctcat tcatttcaac atcatacaag gctatgacat
    16861 ggtgaaccat aaaatttttt aactcttccc tgacgaaaac atcgtagatt cgaggattag
    16921 agtagcttct aacagtaaat cccgtctctt ccataacttc tctagtcagc gtttccgtca
    16981 gtccttcacc aagttgctga ctgcctccag gtagatcata ccgatgttga taagggcctc
    17041 tcgttttttc aatgcaaagt aactttccat tttcaaagca aacacagtag accccaaagt
    17101 gatttttgat ttccatccaa ctcctcctac ttcaaagacc agccaccatc tattgtcaag
    17161 atttgtcctt gcatggcgct cgcttttcca cttgctaaaa aaagactaag ctctgctatt
    17221 tcctctggct caatccagcg cttgattggg gtttcactag ccacccagtc agccaaacca
    17281 cctggttcaa aatccgcagc ggtcatagct gtcttgactg ctcctggagc gataccaaag
    17341 acctgaatcc cagcttcagc atagtctaga gccaactgct tggtgaagcc agccaaggca
    17401 tgcttggatg aagtataggc gtgaccacct ccacctgcta ggctagaagc aatggaacac
    17461 atattgatga tgattccctt tttattttcc agcatttgtg tcaaataata ccgagtcaac
    17521 tctactggaa taatgtagtt gatttcaaaa atctcttgaa tgtcctgcgc cgtttgttcc
    17581 aacagtggtt tgtaatcatc caaaactcca gcagtattac acaaaacatc cacctgaggg
    17641 caccagtcaa aaataggttc caagtccaag gtcaaatctc tctgtaaaaa gcgaaaatca
    17701 ccctctaaga gtggcttttc accttggtca actccataaa cttgatagcc cttctctaaa
    17761 aagaggcgag cttgagccaa tccgatccct gaactcactc ctgtaatgag tacacgttta
    17821 gtcatgcact tctacccaat ccgttgccaa aacatcacaa actgtcgggc tccacatgga
    17881 aaaaccttct ccttcgccag aaacgttgat taggaaataa ggtgtcattt caagtgcaag
    17941 cccattttgc tcgatggtat caaagagttg gacatagttt tccgcacctc cccaaccagt
    18001 tcgtacatat tttctcttag cctttaaccc aggcaggatc tcttcaaatg tcatgttttt
    18061 ctcctttaat tctacattct tcatttaatt atagcaaaaa accgctttat acggcttttt
    18121 gaatgtgagt tattcaaacc tgctactact tacggcaaat tattccctgc agcaagataa
    18181 atttcatacc attcttttct tgttaagcta aagtttgccg ctcggctaac ttctctcaag
    18241 tgcttaggat ttgttgtacc tacgactgcc tgcatttttg ctggataacg caatatccaa
    18301 gaaatggcaa tagttgaaga ggttactcca tatttaatag ctaaacgatc aagtacttga
    18361 tttaaagctt gaaatttctc atttccaaca aaattccctt taaaataccc gaattgtaag
    18421 acagaccatg cttgaatgac cacatcgtgt aattggcaat attcaaaaat gctgccatct
    18481 cgcatagctg cttgactatc ttccatatta acatgaaaag ctgattcaaa tcctggagta
    18541 aaagccgcac tcaattgtag ctgattaaca gctaacggct gcttgacatc ttttttaagc
    18601 aactccatca tcataggatt ttgattagaa actccaaaat ctcgaacttt accttgttta
    18661 taaaggagat taaaggcttc tgctacttgg tcagattcca tcaaagcatc tggtcgatga
    18721 aggagcaagc tatctagatg atcaatcttc aatctttgca aaataccgtc tactgatttt
    18781 ataatatagt ccttagaaaa atcaaaatag gtaaattctt caatgcgaat gccacatttg
    18841 gactgaatcc acatcttttc tcttaaatct ggacgatttt ttaggacaag acctaacagt
    18901 tcttcacaac gaccacgacc ataaatatca gccaagtcga aggcattgat tccaacagaa
    18961 agtgctgttt ctacaagctc ttcaacttct tttacagatt tatcttttat tctcatcatt
    19021 ccgagaacaa tttctgataa ttctttgtca tcttgaccaa gagttatgta tctcatcaaa
    19081 tttttctcct ttaatttcta acattcttcc cttcattata acaaaaaacc gctttgcaac
    19141 gactttttga ctatacttca ctccatttta tcttcttaaa cccacggaac aagacaaaga
    19201 ttccaataaa gaggacagct aaaggaataa cttttgtaag gaaaacattt gaaattccca
    19261 tccactcata ataacggagc agagaaccca ccacaagatg ggcaataatc atactgacaa
    19321 atggacgaaa gaccgcttct ttccaattcc aaataccgat aactagcgaa atcgtaaaga
    19381 cagacaaact atcccaggga gccggaatat aaaaggctcc ttcttgtatg aagcttgcca
    19441 ttcctacata tcctaaaaca actagaagaa ctatagtccc aacaacaatg taagtgccaa
    19501 ttttcatttt aggagaatct tggactaaac ttcttcgtaa aattgtggcc acaagtccaa
    19561 atccaatcag aaaaataaga agttgcccta aaaatgtgag caaattgact gttaagagag
    19621 gacctttaga aaaatcactt agtagttgat aataacgtaa taccgccagg acaagaattg
    19681 gcgtcaaaag ggactctttg atagaactgc gaggtgctcc cttgagaatc tctttcatta
    19741 tttttttagg attcttacct agataatcct ctgcactcat gccatctcgt tctgcttctg
    19801 agaaatctag catcatcaaa tagatctgct ctctgagata gtcttcatca tagagaaatc
    19861 cagcaagatt aaaactttcc cacaactcct caaaatactt ttgattctcc tcagaaaact
    19921 catgtagcaa agcgcttgtt tcttcgtaat acttcatttt cttcatggtt taacccccat
    19981 tcttaatccc ttctactttt tgactcaaat cgtcccattg ttgccaaaag actgagacac
    20041 gctcttctcc ttctttcatt aatgaaaaat acttccgatc tggaccatct ggcgacgggc
    20101 gcatgtcgcc tcttatccat tgattttttt ctaacttttg caacaaagga taaatagttc
    20161 ctggaacgat agtatcaaat ccagcctctc gcaaagtctg aaccaactca taaccatacc
    20221 gctctttttg accaatcata tccaagacac aaccttcaag aacacctttt aatagctgag
    20281 tttctttcat cacttctccc ttctaatcta ttttgtaata cctactagtg acttcaccta
    20341 tagtatatca cttctacact agtttgtaaa gcataatagt taatactctt cgaaaatctc
    20401 ttcaaaccac gtcagcgtcg ccctaccgta tgtatggtta ctgacttcgt cagtttcatc
    20461 tacaacctca aaaacatgtt ttgagctgac ttcgtcagtt tcatctacaa cctcaaaaca
    20521 gtgttttgag ctgacttcgt cagtttcatc tacaacctca aaacagtgtt ttgagctgac
    20581 ttcgtcagtt tcatctacaa cctcaaaaac atgttttgag ctgacttcgt cagtttcgtc
    20641 tacaacctca aaacagtgtt ttgagcaacc tgcggctagc ttcctagttt gctctttgat
    20701 tttcattgag tataaataaa aaaacagaac tagcctgaac tagtcctgtc tacttttacc
    20761 caatcacact tccatttggt acagctggat caactgtgag aagggttaat ttgccatcat
    20821 gttcagctga gagaatcata ccctggctga catatttttt catcatttta cgtggtttga
    20881 ggttagcaat gatttgaact ttcttgccga ccaattcttg ttcatttgga tagtattttg
    20941 caattcctga aagaatctga cgatcttctc catcaccagc atccaagcgg aattgaagca
    21001 acttatctga accttctact ttagacactt ctttgacttc tgcgacacgg atttcaacct
    21061 tgtcaaagtc ttcaaacttg atttcatcct tgtttagttt gagctcaact tcgtccggat
    21121 tccattcttt ttcgactgct ggtttattgc cttccatttg ttccttgata taggcgattt
    21181 cttcttccat atttagacgt ggaaagatag gtgttccttt ggcaactaca gtcacatctg
    21241 ctgggaagtc agccaaactc aagttttcaa gactagaaac ttcttccaaa ccaagttgag
    21301 tcaaaactgc acgactagtt tccatcataa atggttcaat caagtgagca actacacgaa
    21361 tgctggctgc caagtggctc atgacacttg ccaattggtc acgaagagct tcatccttgg
    21421 ccaagaccca tggtgcagtc tcatcgatgt atttattggt acgagagatc agagtccaga
    21481 ctgcttcaag cgcacgtgga tagtcaactg cttccatgtg tgtatggaag tctgcgattg
    21541 attgttctgc aacctcagca agaacatgat caaattcagt cacaccttct acataggcag
    21601 ggatttgtcc atcaaagtac ttattaatca tggaaaccgt acggttaagg aggttcccaa
    21661 ggtcattagc caattcatag ttgatacggc cgacatagtc ttcaggagta aaggttccgt
    21721 ctgaaccaac tggaaggtta cgcatgaggt agtaacgaag tggatctagt ccataacgct
    21781 ctaccaacat ttcagggtaa acgacattcc cttttgactt agacattttt ccgtctttca
    21841 tgacaaacca accatgggca atcaaacgat caggtaattt aacatccaac atcataagaa
    21901 ggattggcca gtagatagag tggaagcgaa ggatatcttt tcctaccata tggaagactg
    21961 ttccattcca gaacttgtca aagttaccat gttcgtcttg agcgtagcca agagctgtcg
    22021 catagttaag aagggcatca atccaaacgt agacaacgtg ttttggattt gatgggacag
    22081 gcactcccca tgtaaaggtt gtacgagata ccgccaaatc ttccaaacct ggctcgatga
    22141 agttgcgtag catttcatta aggcgaccat ctggcgtgat aaattcagga tgagctttga
    22201 aaaattcgac caaacggtct tggtatttgc taaggcgaag gaagtatgat tcttcagaaa
    22261 cccattcaac ctcatgacct gatggagcaa taccaccagt cacatttcca gcttcatcac
    22321 ggaaaacttc tgccagctgg ctttctgtaa agaattcttc gtctgatact gaataccaac
    22381 cagagtattc acccaagtag atatcatctt gagcaagtaa gcgttcaaag acttgtgcga
    22441 caactttttc atggtagtca tcagttgtac ggataaattt atcgtatgag atatctagta
    22501 attgccagag ttctttaact ccaaccgcca ttccatcaac ataggcttga ggtgtaatac
    22561 cagcttcttc cgctttctgc tggattttct gaccatgttc atcaagacct gtcagataaa
    22621 atacatcgta gcccatcagg cgtttgtaac gtgctaggac atcacatgcg atagttgtgt
    22681 aggcagaacc gatatgaagt ttcccagatg gatagtaaat cggcgttgta atataaaaat
    22741 ttttttcaga cataattttt cctttccagg caaatgaaac ctgtttttct aacacttcat
    22801 tatatcacat ttttaatgaa tttcgatagg gaaatccata caaaaacaag atagacgagt
    22861 gtccatcttg ttgatctcat tcataacgaa gggcttcaat tggatcaagt ttcgatgcct
    22921 tgttggctgg caagactcca aaaatcatac caacactagc cgaaactgca agactaaata
    22981 gggcaactgg gattgatact ccaacttcta taccttctat taaaccttgc agtaacaaac
    23041 ctgctaaggc agttaaacca cttgcaattg tcaagccaat taagccacct aacaaggtca
    23101 aaatcatgga ttcaatcaaa aactgaatta aaatattggc acgtgttgcg cccaaagcct
    23161 tacgaagacc aatctcacga gtgcgctctg tcaccgaaac cagcatgatg ttcatgacac
    23221 cagttcctcc aacaaagaga gaaatccctg cgatggaact aataatcgtc gtcataaaac
    23281 taaacgattg ttgaatttct gcaaatacaa cggactcatc tgccacctgg tattctccct
    23341 gttgtaagcc tgcaagctct gtcatttttc gtgccagttc tggacccaga gttggggtta
    23401 aactggtatc attcactcga aagacaatac tagctatttc atctatatta aaattcgcag
    23461 caagggagat attggtagta ataggcaagc caccaaaccc atatattttt gaccttttag
    23521 cctccggact agtataaacc ccaatgaccc ggtaactaaa tccattgact tctacaacct
    23581 tgttaatagc ctcttgagga gattcaaata aactaatgga caattcctca tctagcaaaa
    23641 taacacttgc aaactctttg aaatcttgct ctctcagact acgacctgca ataatttcat
    23701 tcttaacagc gtccatgtaa gttctgtttc cacctgtcaa attagcattc tcaacctttt
    23761 tatcttgata ggtcaagatg gcattcgttg aattggttac atagtaacta tccactccct
    23821 tcagtttagc tgcctcttgg acccaggatt cttgcggttt tggcggttca acaggaattt
    23881 cctcttcctt tccagaaacc gtaaaagatg attgtttctg agtaaaagaa ccatctttac
    23941 tttttttagg agagaaaaag acgctaatat ttttctgaga tttagtcata tctttattga
    24001 cttgacgaga tagggaatca cccaaagcca taatcacaac aactgatgaa acaccgataa
    24061 taatcccaat catagtaagc aaagaacgca tcttgtgagc catgatagat gaaaaggcaa
    24121 atttcagatt ctgcatctta gttttcctcc tttcctaact gagcactgtc agacgaaatg
    24181 accccatccc gaatgacaat ctgacgttta gcataggcag caatctcagg ctcatgcgtt
    24241 accatgataa tggtttttcc ttctttattc aaatcaacca ataattgcat aatttggtta
    24301 cctgttttgg tatccaaggc tcctgtcggt tcatccgcta ggataataga aggattgttt
    24361 accaaggcac gcgcaatggc tacacgttgc ttttgaccac cagataattc tgaaggtaaa
    24421 tggtgactac gttctgtcaa ttcaaccttg tctaaatatt cctcagccaa cttgcgacgt
    24481 tttgaagacg aaactcctgc gtaaatcaag ggcaattcta cattttgcag agcattgagc
    24541 ttcgatagaa gaaagaactg ctgaaagaca aaaccgattt gttggttacg gaccttagcc
    24601 agttgttttt caccaagccc agccacttct tgaccttcaa gataatattc tccactggtt
    24661 ggtgtatcca acatgccaat cgtattcatc agagtggact taccagaccc agatggtccc
    24721 atgatggcta caaattcacc ctcattcact tctagattga tatttttgag aacctgcagt
    24781 tcttggtcac cattacggta gcttctgaag atatttttta gactaattag ttgcttcatc
    24841 agccttcacc tcttttcctt cttccaagga agatgttgga ttactgatga ccttagcacc
    24901 gttcgttaaa ccagaagtga tttcttgatt ttctgcgtca gcatttccca atgaaacctc
    24961 aactttttta gccttttgtt gttcatccac aatccagaca taatttttac tatcatccat
    25021 tactagactg ctaacaggaa caagaatagc cttagttttg cttttaacct caatgttgac
    25081 agaaaaacct tgtttcaaat caccaacctc gcctgtcaca tcaatagtat aagggtattt
    25141 agaacctgta ttattcccgg ctgctggact agctgcttca ccattgtttt taggatagtc
    25201 agaaatatag cttaatttcc cagtccattt tttatcagga tacactttag aagtaaagct
    25261 tacttcttga cctacagaaa ggttggctag attgtactca gacaattctc ccttgacttg
    25321 taaattttca ttgctgacaa tatgaaccat aacttgactc gcccctgttg gagatttaga
    25381 aacattgcta ttgacttcga ccacagttcc ctctagggta ctgagaacag ttgttgcatc
    25441 caattgactt tgagccttgc ttaattgcgc cgcagcatct gcacgcgcat cacgggcatc
    25501 acccaattga gcgtcaatag aagcaacaga atttccagcc actggagttg ggctttgcac
    25561 cgttgcatct tctcctccta ctggcgctgg taactgtgga gccggagctg aagcggcttc
    25621 atttcgtgct tgattgagtt cattgatatg acgatctgcc ctagctactg ctcgactagc
    25681 tgaatcatag gccgcctgcg cttctgaact actgtacttg actaaagcct gcccttcgct
    25741 gaccttatcg cccacagaaa caaggatttc atctaaatca cccttactag catcaaaata
    25801 aacatattgt tcattttttg ctgttactgt ccctgacaat aaaacagagg aggccacgct
    25861 tccttccttg gcaacaacaa gatgagtagg ctcatctttt agagcagtct gagaaggttg
    25921 tctaaagagt aaaatccccc cagcacccaa tacaactaca ctcgcagcac cgattgctgc
    25981 atacagttgc cactttttag ctttaccatt ctttttcttc ataatgaaac tccttttctt
    26041 ttttacaata ctttgctatt ataccaaatt tccctccagc aaacaataca gttcaggatt
    26101 aaacaatcgt tcggaatttt gcttttctat tgtactagat atataataca ctatcttgac
    26161 ttatttggca agcttttttg caattttttg tgcgtagcag atttttgcaa tcaaatcaaa
    26221 aaaagaataa aatataacta tcaaaaaaga cgctctacta tttaaaagat tacaaaggag
    26281 ttttcaatga gagaatatga tatcattgct atcggtggag gtagcggagg aatcgctacc
    26341 atgaaccgtg ctggtgaaca tggagccaaa gcagccgtta ttgaggaaaa gaaattaggt
    26401 ggaacctgtg tcaacgtcgg ttgtgttcct aaaaaaatca tgtggtacgg ggcgcaaatc
    26461 gctgagactt tccatcaatt tggagaagac tacggcttta agactactga tcttaacttt
    26521 gactttgcaa ccctacgtcg caatcgtgaa gcctacattg atcgcgctcg ttcttcttat
    26581 gatggtagtt ttaaacgcaa cggtgtagac ttgattgaag gtcatgctga atttgtagat
    26641 tctcatactg taagcgtaaa tggtgaactg attcgtgcta aacatatcgt gattgctaca
    26701 ggtgcccatc caagtattcc taatattcct ggtgctgagc taggtggctc ttctgatgat
    26761 gtatttgcct gggaagaact tccagagtca attgccattc taggcgctgg ttatatcgcc
    26821 gttgaattag ctggcgtact ccacactttt ggtgtcaaga cagatctctt tgttcgccgc
    26881 gatcgtcctt tacgtggttt tgattcctac atcgttgaag gtttggtcaa ggaaatggaa
    26941 agaacaaact taccacttca cactcacaaa gtccctgtca agttagaaaa aactactgac
    27001 ggcattacca ttcatttcga agatggtact agtcacacag ctagccaagt tatctgggct
    27061 acaggtcgcc gtccaaacgt taagggcttg caacttgaaa aagctggagt gactctgaac
    27121 gaacgtggct ttatccaagt ggatgaatac caaaatactg ttgttgaggg aatctatgct
    27181 ctaggtgatg taacgggcga gaaagaactg actccagttg caatcaaggc cggacgtacc
    27241 ctatctgaac gtctctttaa cggaaaaact actgcaaaaa tggattactc aactattcca
    27301 actgttgtct tttcacaccc tgctatcgga actgttggtt tgacagaaga gcaagctatt
    27361 aaagaatacg gtcaagacca aatcaaggtt tataaatcaa gctttgcatc tatgtactct
    27421 gcttgcactc gcaaccgtca agaatcccgt ttcaaactca taacagctgg ttcagaagaa
    27481 aaagttgtcg gacttcatgg aattggctac ggcgttgatg aaatgattca gggatttgcc
    27541 gttgctatca aaatgggagc aaccaaggct gactttgatg caactgtggc aattcaccca
    27601 actgcatctg aagaatttgt aaccatgcgt taatcgaaac aaaagaagct gatataaata
    27661 tgtctgcttc ttttttaatg attttcgaat caacaaaaaa attatccaac ttgactagtc
    27721 acgaaaatcg gctgttacag tttttgttac cttgttttta aaaaatggtt gacatcgact
    27781 cctatatgag tataatagtt actatacatt agaaaagagg ttaactattt tgaaaaaagc
    27841 tcacgtttat gctatccctg ctattggggc tgctctcatt gctgctttgg cacaaatcag
    27901 tcttccaatt ggacctgttc ccttcactct gcaaaacttt gcaatcggct tgattgctac
    27961 tgtctttaga ccgagagagg ctgtactttc tgctggactc tatcttcttc taggtgctat
    28021 cggtcttcct gtctttgcag gaggtggagc tggttttcag gctttagttg gccctactgc
    28081 aggctatctt tggttttatc tcgtttactc tggacttact tcctctctaa ccaacagcaa
    28141 gagtggtgtt gttaagattt ttcttgcaaa cctcttgggt gatgcccttg tctttgtcgg
    28201 cgggattctc agcttgcatt tcctagctgg aatggcattt gaaaaagctc ttgctgtggg
    28261 ggttcttccc tttatcattc cagaccttgg caaacttcta gctattagtt ttattagccg
    28321 tcccctactt caacgcctta aaaatcaggc ttactttact aactaaaaaa ggatatcgag
    28381 ttatcatgac tcaatatcct tttcttttat tttgaaaact tatactcaat gaaaatcaaa
    28441 gagcaaacta ggaagctagc cgcaggctgc tcaaaacact gttttgaggt tgtggatgaa
    28501 actgacgaag tcagtaacca tacatacggc aaggcaaagc tgacgcggtt tgaagagatt
    28561 ttcgaagagt attagttgcc tttaaaggca tccaccatcg tttgaaattc ttcatttgag
    28621 agagtaatcc ctttgcccat tttagtatgg tctggactcc aagcacgaat atcaaacttt
    28681 gcaggggcac cattaaagct cacacggtta atttccttgg tccaaccttt ttcgttttca
    28741 gaaagagtca acaagtgctc ttcgatttca aatgtaaatt ctgccatttt cttctccttt
    28801 tttagtttca ttagtttatt cgtaaaatct tgtagatttt aggaaaattt tatataatat
    28861 tgatataaaa gaagggaggc caatatgaga cataaattcc agcaagttct aaataaaata
    28921 catgattttt taaatggata tgaccaacct gaccagactg aaaccaactc ccttacagcc
    28981 actattgaag aggctatcca gaaacaaacc gctgttcacc ttatcttgtc tgagacaagc
    29041 tttacaggtg acatcatcaa atatgatcag caaggccagc aaattatcgt gaaaaatttt
    29101 tccaaaaatg tgagccggat tatccgtata agcgatattc aacgcctgcg atttgtcccc
    29161 tcaactgtcc aaacagccca aaaaaataga tttaagaaag agtgagatgt agttgcttca
    29221 tcccactctt ttttcttagc gaatttgttc aaaatgtaaa tgaactgcga tatgatctcc
    29281 ataaccactt ctttccaagt cacgttgtaa acgataggaa atgtagtgtt ctgcaatggt
    29341 aatgtaacct gcgcccaata aacgatgttc aaccatagat tgaatcatac tgatagtcgc
    29401 acgttccacc ttggcttctt gtaaatccaa aactaccttc ttagtgactt gagcaagatt
    29461 ttgacgcaaa tcatctgtca aaacataaac agtttgggct gccttcaaga tggcttggta
    29521 aatcttatct ggattaaatt cagcaatttc gccattacgt ttgattactt gcataggttt
    29581 ctcctttatt ctttgttttc tttgatttct gccagcattt tttcttcttc tactgtcagt
    29641 tgataatgtt caagtaaatc cggtctgcgc tcgtaggttt tctttaaact ctcgtacaat
    29701 cgccactgac gaatcttttc atggtggcca ctcatcaata catctggcac gaccatgcct
    29761 cgataatcat agggacgtgt gtactgagga tattctaaaa gacctgaaga aaaactatca
    29821 tcttggtggc tagactcctt gccaatcact tctggaatca ggcgaactgt agcatcaatc
    29881 atggtcatag ctgccaattc tccaccagtg aggacatagt cacctaggga aatctcatct
    29941 gttaccaagg tcttaatgcg ctcatcataa ccctcatagt gcccacagat aaagattagc
    30001 tcttcctctt gagccaaatc ttcagcataa gcctgatcaa actgctttcc agcaggatca
    30061 aggagaataa cgcgcggatt tttcttttca atagcatcaa aggaatcgaa aataggttgt
    30121 gctctgagca acatgccctg accgcctccg tagggctcat catctacatg acgggccttt
    30181 tcagcatttt ctcgaaaatt atgatactgg atatccaaga gccctttttc tcgagccttt
    30241 ccaacgattg agtgctccag tggagaaaac atctctggaa agagggttaa aatatcaatc
    30301 ttcatcgtct aacccttcta agatttccac atcgacccgt ttacttggaa tatcaacatt
    30361 gagaaccact ggtgggatat aaggtaaaag caaatcacgt ttgccttttc gtttgaccac
    30421 ccagacatca ttagcacctg gttgcaggat ttccttgatg gttccaacca agctatcacc
    30481 ctcatagact tccaaaccga taatctcgtg atagtaaaat tcaccatcgt ctaggtcatt
    30541 caaatcttcc tcagcgacct tgagactgta tcccttgtac ttttcgatag tattgatatg
    30601 gtacatatct ttgaatttaa taatgtcaaa gttcttctgt ttacggtggc tagcgatggt
    30661 cactgtttgg acaaactgat ctttttcatc aaacaaaacc agctcagctc cttttttaaa
    30721 ccgttcttct gcaaaatccg tcacagacaa gactcgcatc tccccctgta atccctgcgt
    30781 attaacgatt ttcccaacat taaagtagtt catcttgtct cctgtaatct ccttttttcc
    30841 atcttattct aacaattctc gaataatagc cgcaattttt tccgattctg accattgtaa
    30901 ataatggtga ttccctccta aaatgagttt agtattggaa gtccaatatt ctgattctct
    30961 gtactctttt tctctataag gctgacaaaa aacaaataca ggaatatgag cttctataga
    31021 tacatcctca aaatcttcct cagtaatctc tccagatatc tgaaattctg gatcttgatt
    31081 ttccaactct aagccttttt cttgcattaa ttcccagatt tttttattcg tttcaggact
    31141 aaatgttgct tgagttaagt tcttaaaata aagttcagga ccacactcgt caatcagcct
    31201 catctgctct tccatttctg gataaggatt ttctgaaaaa tcagcaaaca tgactttttt
    31261 agttgtcggt tcaattgcta ctaaagtctg acgcttaatt ggtttctcga gtaatttgca
    31321 agctaaaatt ccactccaac tatgtgcaca aagtatatat tcagaaattc ctaattcttc
    31381 aagtacttca taaaccgcat ctgcaagatt atctagattt tttccagctt ggtcatgaat
    31441 cggactccta cctgtgttcg gaaaatcaat tgtcaaataa ccaattgtag gaggaggttt
    31501 ttcaagtata agtgaaaaat tttcataact tggtagcaaa cctgctccgt ttaaacaaac
    31561 tagcactttc ttttgctttt gataagtaac agagaggcta ccaatttctg tagatacttc
    31621 aaacctcttc ataaagaaat ccactgattc tatataatga attattaaaa atccttatcc
    31681 tttattttat cacgttccaa ggattttctc aagttggagg aaggggacaa tatctctact
    31741 ttcccttcaa taatccttcc aaattatgtt tatgttggta attaatggct gcggttttgt
    31801 ctttctcaaa gacagtcttg gtaaggtcaa tatgattaat agctacgatt gcgacggtgt
    31861 agtaaatgat atcagccagt tctctggcaa gttcctcgtt cgaatcctat cccttctttt
    31921 cgaccagagc gcctattcaa aacctcgact acttctccga cttcctccac taacttcata
    31981 aagagacctt catcagtccg agactgctgt taatgttcga ttaagtagtc ttggaattgc
    32041 ctaaacgttc aatcttttat agtatattga aactagaata gtacaccttt acttctaaaa
    32101 cattgttaga aatcgatttg actgtcctga tcgatttgtc ctgttcttgt ttcattttac
    32161 tatatcttct attccacaca aaaaagcgag acatccgtcc cgcccttctt atttttcgtc
    32221 aataacgatt cttacttttt tgtattcagt tgggacagag tagacaatcg ttcttatcgc
    32281 agaaatagtg cgacccttac gaccgattac acgacccaca tcgctttgat caagattcaa
    32341 atgatattcc aaaaattctg gtgtatcctc aatcttgata gttaaggcat ctggttgtga
    32401 aattaagggt ttcacaatcg caataatgag attttcaatc gtatccatct gtcaacctac
    32461 tttaaactta ttttgaaaat ttagaatcgt ggaatttttt caatacgcct tcttttgaaa
    32521 ggatgttacg tactgtgtct gaaggttgag ctccattagc caaccatgca agaacgcggt
    32581 cttctttcaa agttacttgg ttttcagcaa caagtgggtt gtaagttcca actgtttcga
    32641 tgaaacgtcc gtcacgtggt gaacgtgaat ctgctacgtt gatacggtag aaaggttttt
    32701 tcttagaacc catacgagtc aaacggattt taactgccat ttttaaagtc tcatttcttt
    32761 aattttttat ttcggtgaaa tagctgagct atttagcaca tgttctatta tagcagattt
    32821 ctggcatgtg tcaagaaaaa aacttgacag actataaatt tcttaaagta tttagaaatt
    32881 tgttagaata ggaaataaat gtttgaaaga tgctatccgt gaatactatt ttagaaaaat
    32941 tttataaaga acaccaagtc aaacccttca tatcgcctga acgagagttg gatacttggc
    33001 ttctaagtcc caagcccgtt cccaagcgaa atatggactt attagtagat gattcactag
    33061 caggagatat cattttacta tggagaatcc agtttggtac ttttaccact gaaacatgat
    33121 tttaaaaact cccattcgaa tatgattcaa aattcgctta gaatcaatgt tttcccactt
    33181 tttcaaaaaa taaattttca tctaatttaa ataactttca attaagtgtg aattttgctt
    33241 aaaatcatta aaaaacaccc catggtgtga accatgaagt gttgtaaatg ctgtgttgta
    33301 gctagttctt agcgtttaga gaactggcta gctttacgag ctttcttaag atctggtttc
    33361 caaaatactt tgagtacact tatggtattg atgcccctaa acatctaaaa actctggtag
    33421 aaaaagggta tgctcttgtc gaaacttctt ttgactcact tgaccatcta aataatacca
    33481 tgaaaaagaa tatcctgaag aagaagggag ttactggact ttctaaaatg aaatcttgcg
    33541 acctggatca agcccttcat gagcattttt cagaagaaga attagctggt cactttcatg
    33601 tccttctatg gacttttttt acaatggcat tgctatcaca cccaatacct atctaagcgc
    33661 ctggttcgta aactttattg cagctcttcc tctaaatttc ctaattgttg aaccaattgc
    33721 ccgttttata ctaagttctt ttcagaaacc atttactggg gaagaagttg aagattttca
    33781 agatgatgat gaaatcccaa ctattatcta agccagttct gtaaactact aatatttgaa
    33841 atccacttcc ttttagggta caatggttat aaatgaattt ttgagaggat cagaatgaaa
    33901 aaactagcaa cccttctttt actgtctact gtagccctag ctgggtgtag cagcgtccaa
    33961 cgcagtctgc gtggtgatga ttatgttgat tccagtcttg ctgctgaaga aagttccaaa
    34021 gtagctgccc aatctgccaa ggagttaaac gatgctttaa caaacgaaaa cgccaatttc
    34081 ccacaactat ctaaggaagt tgctgaagat gaagccgaag tgattttcca cacaagccaa
    34141 ggtgatattc gcattaaact cttccctaaa ctcgctcctc tagcggttga aaatttcctc
    34201 actcacgcca aagaaggcta ctataacggt attaccttcc accgtgtcat cgatggcttt
    34261 atggtccaaa ctggagatcc aaaaggggac ggtacaggtg gtcagtccat ctggcatgac
    34321 aaggataaga ctaaagacaa aggaactggt ttcaagaacg agattactcc ttatttgtat
    34381 aacatccgtg gtgctcttgc tatggctaat actggtcaac caaacaccaa tggcagccag
    34441 ttcttcatca accaaaactc tacagatacc tcttctaaac tccctacaag caagtatcca
    34501 cagaaaatta ttgaagccta caaagaaggt ggaaacccta gtctagatgg caaacaccca
    34561 gtctttggtc aagtgattga cggtatggat gttgtggata agattgctaa ggccgaaaaa
    34621 gatgaaaaag acaagccaac tactgctatc acaatcgaca gcatcgaagt ggtgaaagac
    34681 tacgatttta aatcttaaaa accaaaaaaa tacagtatcc acattcggta ctgtatttct
    34741 tttactctca ttcttaagtt aaattattaa aatcccatat ttggtctatc cagccttcat
    34801 aaaagtctgg ctcgtggcag accataagga tagatcccct atattctttg agagcgcgtt
    34861 tgagctcatc ctttgcatcc acatccaaat ggttggtcgg ctcgtccagc actaaaacgt
    34921 tgttttcacg attcatcaag agacagaaac gaaccttggc ttgctctccc cctgataata
    34981 cttgaatctg gctttcaata tgtttggttg tcaaaccaca acgggcaagg gctgcacgga
    35041 cttctgcttg attaagggca ggaaaggcat tccagacagc ttcaagagga gtttggcgat
    35101 taccgccttc tacttcctgc tcaaaataac caagttctaa ataatctcca cgctccactt
    35161 ccccagcgat tggcgagata atgcccaaga gactcttcaa gagagttgtt tttccaatac
    35221 cattagcacc aataatcgca accttttgat tgcgttcgaa ggtaagattt aaaggcttag
    35281 taagaggacg gtcgtaacca atttgcaagt tcttggcttg gaagataaag cgccctggtg
    35341 tacgagctgg tttgaaatca aaggatggtt ttggtttctc actttggagt tcgataatat
    35401 ccatcttatc caatttcttt tgacgagaca tagccatatt acgagttgca acacgggctt
    35461 tattacgagc cacaaagtcc ttgaggtctg caatctcttt ctgctggcgt tcgtaggctg
    35521 cctctagctg agatttcttc atagcataaa cttcttggaa ctggtagtag tcaccagagt
    35581 aacgcgtcag ctgttgattt tccacatgat agacaatatt aataacgtca ttgaggaatg
    35641 gaatatcgtg cgaaatgaga acaaaggcat tctcatagtt ttggagatag cgcttgagcc
    35701 aatcaatatg ctcagcatcc aagtagttgg tcggctcgtc caacagcaag atatcaggct
    35761 tttcaaggag aagttttgcc aaaagcacct tggttctttg cccacctgac aaagaagtta
    35821 catccgtatc catgccaaag tccataacac caagagcacg cgctacttcg tcaatcttag
    35881 catccaaggt atagaaatca cgactctcca gacggtcttg aagttctcct acttcttcca
    35941 tgagagcatc aacatccgcg ccgtcttcag ccattttcat atagaggtca ttgatacgag
    36001 cttcagcttt gaaaagctca tcaaaagccg tacggagaac atcacgcacc gactgtcttt
    36061 cagcaaggac agagtgctga tccaagtaac cagccgtcac atatttggac cactcaacct
    36121 ttccttcatc tggcagcatt ttaccagtca cgatactcat aaaggttgat tttccttcac
    36181 cattggcacc gaccaggccg atatgttctc ccttgaggag acggaaggac acatcttcaa
    36241 aaattgcacg gtcaccaaaa ccgtgactca gatttttaac ttctaaaata ctcattttaa
    36301 ttccttacct tgtttttatg taatcgttta taaaggagcc aagccagata gccacccaaa
    36361 gtgttggtcc acaaatcatc aatctcaaag acgcgattga aatcaaagaa aaagtccaag
    36421 attaattgcg tacactcgat tccaagactc acaagaaaac taaaaagaag gacctttttt
    36481 gttttccgca aatttggaaa tagataaagg agttggaaaa tcagaggaaa aaacaagaag
    36541 acattgagga tattttgtaa aaaaatccaa cataattgtc caatgtcact cacttcgccc
    36601 agtttccaga gagaattgaa aggagtcaaa agaaaaacca ggcgtccaag atgctgaata
    36661 cctggagttc ccactcccac ggtagattgt tcttgaggag taaagcaaaa acagacaatg
    36721 caaatgctat agaaaatgac tccccagacc aaaatatgat tataagtctt cttcatcatt
    36781 aaggatttac cgctgcgact gccttctggc ggtcacgttt cattgtgtta gagcgcaatt
    36841 gtccacaagc tgcgtcaata tctgtaccat gctcttgacg aaccacacag ttgacccctt
    36901 ttttcttaag cgtatcatag aaagccaaca cgcactcttt gggactacgg ctatattggt
    36961 catgctcact aactgggtta taaggaatca agtttacata agacaatttc ttgatgttct
    37021 tgagcaattc agtcaattcc aaggcttgtt ctacaccgtc gttgacttca ttaagcatga
    37081 tatattcaaa ggttacacga cggtttgttg tctcaatgta gtattcaata gcagcaaaga
    37141 gtttttcaat cggaaaggca cggttaatct tcatgatact tgaacgaagt tcattgttag
    37201 gtgcgtgaag agacacggca agattgacct gaaccccttc atcagcaaag tcacgaattt
    37261 tatgagccaa acctgaggtt gaaaccgtga tgtgacgagc accgatagcc attcctttat
    37321 catcattgat agtacgaaag aaattcaaga cattgttgta attatcaaag ggctcaccga
    37381 ttcccatgac aacgatatgg ctgatgcgtt catcctgacc acgctcatca aagtatttct
    37441 gaaccagcat gatttgcgct acgatttcac cgttattgag gtcacgttgc ttcttaatca
    37501 aaccagaggc acagaaggta caaccgatat tacagccgac ctgagtggtc acacagacag
    37561 ataaaccata gtgttgacgc atgagtacag tctcaattaa cataccgtcg ggcaattcaa
    37621 agagatattt gactgtacca tcagcagact cttgcacaat acgttgtttc aagggattga
    37681 ccacaaactg gtcattgagc ttagcaatca aatccttgga aaggttggtc atttcttcaa
    37741 atgactgcac acgtttacgg tagagccatt cccagatttg atctgcacgg aatttctttt
    37801 ctccctgctc caatacccat tcctgcatgg tttgatgtac caaactatga attgagggtt
    37861 tcatttcttc tccttattct ctactcactt ctgacgaatg acaaaatgac gttgtccctt
    37921 gtcgtctttc tgacgacgtc tatttttctt atctgcattc gactttcgtt tagtttgagt
    37981 cggtttcttt ccttttctag aaggtgtttc ttcttccgtc ttacgcattt tcttgtcaaa
    38041 tgatgctcgc ttaggggctt cattttctaa gacaaaatag gcacaaccat aactacaata
    38101 ctctaaaagg tagtcttgta aacgactgat tttttcaagt ttttcttctg ttcggtcatc
    38161 cttgtaaaaa cctcgtaggc gaagctgttc gttgctccag tcccccacga tataatcaaa
    38221 cttggttaat acttctgaaa aacgctgatt aaaagtcgtc acatcaaagg catccttgat
    38281 attttcaacc aaggaaaaag ctatcccttc cgtttcgacc ttgtccccgt gtaaatggaa
    38341 ctccggacca ggaaacttgt tatagttgta taattcaggt gcaatttctt ttcgcataga
    38401 tatccttttt tcacgattac ttaatacttt attctaccat aatttctagc agttagcacg
    38461 tttctcataa aaatgaaaaa agtctgacga ttttgtcaga ccagaatctt ataacctaaa
    38521 aagagaagaa caattcttcc ctccaactat cattatttag cagctgcgta caattcatct
    38581 actttattcc agttgattac tgaaaagaaa gctttgatgt agtcaggacg cacgttgcgg
    38641 tatttcacgt agtaagcatg ttcccaaacg tccaagccca agattggttt tttaccttct
    38701 gagattggtg tgtcttggtt tgctgttgaa gtcacttcaa gtttcccttc tttgttgaca
    38761 accaaccatg cccaacctga accaaaacga gttgttgctg ctgcagtgaa ggctgcttgg
    38821 aattcttcaa atgaaccaaa tgttgcatcg attgctgctg ccagttctgc tgaaggagct
    38881 gttttctcgg gagtcatcaa ttcccagaaa agagcgtggt tcaagtgtcc gccaccattg
    38941 ttgataagtg cttgacggat atcagctggg atagattcta catcagcaag caaggcttca
    39001 aggtcttcac cgatttcagg gtgtttttct aaagctgcat tggcattgtt gacataagtt
    39061 tgatggtgtt tgtcatggtg caaatgcatt gtttccgcat cgatgtatgg ttccaaagcg
    39121 tcgtatgcat atggaagttc tggtaagata atagccatct gtaatacctc tttttctttc
    39181 tatatgaaaa tgataacgca aacattcact tttttcaagt tttttgctta tagataagga
    39241 aatcactgac atactttcta ataaaataag caaatcagca agaaacccac gacaagatag
    39301 tcctgacgcg ggttgtagtt tcaacaaatg tcaattgacc tgactagcaa tctgtaagag
    39361 tgccttttca aaaaggaaac ctttttcata aagacctgtc ttaatctgat agtctgtctc
    39421 aatcaaatag gaaatagctt gcttcaaaaa gctcaaagaa agtcctctcg aatctcttaa
    39481 tgcaaacttg atttgataag gatttgggtt acgtcccaga taactaccta aactacttgc
    39541 aatctgcgat tctgtttggc cagactccgc caaaatcttc acctgagtaa aagtccgaaa
    39601 ttgtcccagc atgactgcaa tcagtttgat ttcatcttcc ccttgcaagg tcaagtctct
    39661 caccaaatcg cgcgcctgat ccatcttttt agtcagaata aactgagtta aatcaaaaat
    39721 attgtcctgc aaggtcttgg gaattgcgtt aacaatatcc tcttcctcaa taacagaatt
    39781 cgccttatag gactgtaaaa agagaagatt tttctggatt tcgctaaatt gaaaccccga
    39841 cttgatgagg agattttcaa aagaatgatt ggtaaactgc agaccttgtt tctgactcca
    39901 cttttggaag tactggcgca attcttgttc ttttacttct actgcatcga aggccttggc
    39961 atcacgctta agtaatttga ctaaccgtct tttgctatcc agctttcctt ctgcaaagat
    40021 tatcaacttg gttgttggag aaggattgtc aaggtattcc tcaaatgact taagctcatc
    40081 atctgtcaaa aagcgtttct tagcagtcgt gatatccata aaataatcta atatcacgat
    40141 tttctcatcc gcaaagaaag gaaggctgac caactccagt tccacatcct tgtaaactac
    40201 ttctttcata tcaaagtagg caaagttgag gtcagcagaa tcatacccaa tctgtttcaa
    40261 catttgactc ttcatcactt caaactgacc ctgatctgtc cctgtaaata ggctcaggct
    40321 cggtaaattt gataaagtca acttctgact ttcttcaatg gctagcatcg tctctccttt
    40381 cttcagattt ttcgatttaa tttagtcaat atagcgcaat ttcccacgga aatcttctaa
    40441 gctctcgtag cctttttcca ccatgattgc tttcagttca ttggtaatgc ggtcaaaagc
    40501 actgacgcct tctttgtgaa gggtcgttcc cacctgcacc atacttgctc cacagaggat
    40561 gtgttcaaag gcatctcgac cagtcagaac gccacctgtt ccgataattt ggatttgagg
    40621 atttaaacgt tgataaaagg cgtgaacatt ggctaaagca gtcggtttga tgtattctcc
    40681 accaattcca ccaaaaccat tcttaggccg aataacgaca gattcgtctt ctatatagag
    40741 gccgtttccg atagagttaa cgcagttgac aaacttgagc ggatatttgt tgaaaatagc
    40801 tgccgcttgg tcaaagtgaa caatatcaaa ataaggtggc aatttaattc caagaggttt
    40861 ggtgaagtaa gcaaacactt ctgccaaaat ccggtctgtt gtctcaaaat cataggcaat
    40921 ctgaggttta cctggaacat ttggacagga aagatttagc tcagtcagac cacgaaaatc
    40981 actctcttgg acttttttca aaatagtatg ggtttcctct ggagacatgc cgaccagaga
    41041 taagaagaaa gttcggttcg actctttttc ctgcaaatct aaaagataat ccaaataata
    41101 gtctaagcca ttatttggca agcccataga gttgatggaa ccaagtggaa catcttggta
    41161 gcgtggctca ggattcccct gacggaagtc caaggtcgct gtcttagtaa caaaggttcc
    41221 tgccgctgag tttttgacct cttctaactc ctctatcgtc atacaagcca cacctgctgc
    41281 attcatcaag caattgtcaa actcaaaacc agcaatttgt gttttcgttg ataccatgat
    41341 tctaatcctc cagatttctt ttattgctaa tattatacca taattttaat tctaggttta
    41401 ctagattctg tggttctatc tatttattcg gaaaagaaag tctagtacag aagtcatgct
    41461 atggaaacag ctagtgtatt agaagtttga acaaggcttg ggtaacagac ttttgaatga
    41521 agtccaagca acaaggagaa atctcaacca tgagtccatt ccatgactct gttgctctag
    41581 aaaaccttgc aatagtcaag tgataacgaa taaaactttg gattgaaata tccccaagat
    41641 tatctattac gaggagaaat cttaaccttt atcattttta aagaaatata gtaaaatgaa
    41701 acaagaacag gagaaatcga tcaggacagt caaattgatt gctaacaatg ttttagaagt
    41761 tgaggtgtac tattctagtt tcaatctact atacatgtaa atctctactt tcgcaaaatg
    41821 gattttcaga acagtatccc agtatagtta aaaactataa aaaagagagg acgttcccct
    41881 cttaaaatct tattttactg cttctttcaa agcatctacc ttatccaaac gttcccatgg
    41941 aagatcaata tctgtacgtc ccatgtgacc gtaagccgat gtttgacggt aaattggacg
    42001 cttgaggtcc agcatttgga taatccctgc agggcgaagg tcaaagattt gacgagccgc
    42061 tttttcaagt tgactttcag ctactgttcc tgtaccgaaa gtatcgatac gaacagaaac
    42121 aggttgcgca acaccgatag cataggccaa ctgcacttct gccttcttag caaggtctgc
    42181 tgcaacgata ttcttggcaa tatagcgagc cgcataagag gctgaacgat ccaccttagt
    42241 cgcatcttta ccagagaagg caccaccacc atgacgagag tagccaccat aagtatctac
    42301 aataatctta cgaccagtca aacctgagtc cccttgagga ccaccgatta caaaacgacc
    42361 tgtcggattg ataaagaatt ttgtcttatc atcaagataa gaagatggaa taacttcttt
    42421 gatgaccttg tcaatcacat cttgatggat ttgttcatta gtggcctctg gatcatgctg
    42481 agtagaaata acgactgtat ctacacgtac cggacggtca ttttcatcgt actcaactgt
    42541 aacttgtgat tttgcatctg gacggagata gctaatttct ccagacttac gaagttctgc
    42601 cagacgacga accaatttat gactgagtgc aattggcaat ggcataagct cttctgtttc
    42661 atctactgca aatccaaaca tgagcccttg gtcacctgct ccaatcaagt ccagtggatc
    42721 ttgatcagca tttccacgaa cctccaaggc ttcgttaaca ccttgagcga tgtcaggaga
    42781 ttgttccacc aaagatgggt gtactcccac cgtctcagca gaaaatccat attctgtatt
    42841 ggtataacca atctctgcaa tggtatcacg aaccacacgg ttaatatcca cataggcatt
    42901 tgtagaaatt tcaccaaaaa cgtggacaga accagtatat acagctgttt cagcagcaac
    42961 gtgcgcctct ggatcctttg ctaaaatagc atccaaaatc gcatctgaaa tttggtctgc
    43021 aatcttatcc ggatgcccct cagatacaga ttcagacgtg aataatttac gctctgacat
    43081 aaaaatgtcc ccccttaaaa aatattgtta tagagttaca aagtactgat aggaaaactc
    43141 ttcgtaataa atacctacca tcttatcggg actatataac tttatcatta taccattttt
    43201 aatcgcaatt tgcagtcttt aatgtagtgt ttcggtttag atagttcgtt ctcataaata
    43261 aaaaagttgg agctcaatac tccaacaatt tctattaaaa tgtttggcgt ttagctttac
    43321 gctctgcacg accgcgagcg cgattttcag cacgcttggt tttacggcgc ttttcatcaa
    43381 ccgcccattg aattttcttc ttataacccg gtttgacttt tttctttttc tttttaacca
    43441 aaccaatcat ttcgatatca agtttatctt gttttttctc acggttggca cgacgatcac
    43501 ggtcataggt atcttgaaat tccccgtctt tgaccatctt aggactaaac ttgattccca
    43561 atttctccaa ctcacggata tccgagtcat cacttggctg ataaagggta atagctgtac
    43621 ctggtaggcc atttcgtcca gtacgaccaa cacgatgaac aaaaaaagat aagtcttgcg
    43681 gaatggcatc attgatgaca tggctgacac cttcaatgtc aatcccacgc gctgccaaat
    43741 ctgttgcgac aatatactca aaatccagat tttgcacctg attcatgatt cgcttgcgtt
    43801 cacgaggggc aatatcgcca tggatttttg caaccttcaa gccttgagca gtcagatatg
    43861 aatgcaattc atcagcacgc gttttagtgt taacaaaaat cattgccaaa tacggctgca
    43921 tcaactgagt caactggtaa atttgagcat tcttatcatg tcccttggtc gaaatcaacc
    43981 aattatcaat ggtgtcagaa ataaccgttt tggtcttaat tttctccata acaggatttg
    44041 ataagtattt tttcaagaat ggttgcagtt tttgtgggat agtcgctgag aagaccatga
    44101 attgcaagtc ttttggaaga ctgccagcaa tcttatcaac agtttccaag aatcccatat
    44161 ccaaggtcat atctgcttca tcaacaacaa atgtcttggc tttatgaata gctaaatcac
    44221 cagatttaac caagtcgtag atacggcctg gtgttccaat aacaatatga ggctgattgc
    44281 ttgccaattt ctcaatctgg cgagccttat ccgtaccacc cacataatta accacacgaa
    44341 cttcgacatc tgagtgagct gaaatctgac gcgctacttg gtaaatttga gtagccaact
    44401 cacgactcgg tgcagtaatc actgcttgta cactatcgct agcttcatct aattgctgga
    44461 aaatcggtaa caagaaagta tgagtcttac ctgaacctgt ttttgattct cctactaggt
    44521 cacgacctgc caaaacaata ggaatcaact tgtcttgcac ctctgttgga gttgtaaatt
    44581 ttaactcctt caaggcttct ctaatatagt ttttaaattg aaatttcgta aatgacataa
    44641 catcctcgat tctatctacc ttatcaatta taccatattt tattccatta cagtagtctc
    44701 acttatttag gctatttcca gtagcttctc tagtaagaaa aggctggaat tttatagttc
    44761 caacctcttt tcagttatta tttccagttt aatatagcat tcaagccata gtgatcactc
    44821 acttgtggac tcttgttacc atcaaatacg acatgtaaat tttccaccgc taactctttg
    44881 gtagtaaaga cataatcgat tcgaaggggt tcagtgttcc ctttccagcc atcaatttca
    44941 ggcggaacag tatagctacc acttttctct tgagcaactt caaatgcgtc ttgtaagcct
    45001 aatggactag ctaaaatagc ttggtaacct tcctgtccag ccggattgtt gaaatctcca
    45061 gctagtaaaa gtggcttgtt caattttttc aagacagcct caaatcgtgc ccattcttct
    45121 tggaaacctt tatcccacca agagagatga acactggcaa ctgctagctc cttgccatcg
    45181 actacagttt cagctagggc aacacggcga gtatgatagt ctgttggatc atccacatct
    45241 gaaaccaaaa tttctctggc ttcaataggt gttttagaca agatagccac accttcgtgg
    45301 tagcggttat agccgatatg gttataggcc caggtccagt agtaattttt cccttgctca
    45361 gacaactttt caaccaagag tctaacataa tggtcttggt gaataggctc agctgctggc
    45421 aaagcttgat aaaggtcatt aacctccacc tctgacgagg tcatctcctg attgatttct
    45481 tgaaaacaaa tcaaatcata gtccttttca agaatatctt caagcaaaat ctggaatttt
    45541 tcctctgctt ctttctccat ccaactgtga gtattgagtg ttaaaaattt cattcttttc
    45601 tcccaaaata agaggttgga atacagcttc caacctcaag tatattacaa ttctacttta
    45661 gcaactgctg ttttagctgc aagagaacct gttttttcta acttaactga tttaattgca
    45721 tcaccatttg tgaagacaac tactgttgaa gtttcacgtc ctgctgcacg gatagcatcc
    45781 aagtcagctg tgacaaggag atctcctgct gcaacttttt gtccttcagc aacatgaact
    45841 gtaaatggtt taccttcaag acttactgtg tccaaaccaa tgtgaaccaa tacttcaaga
    45901 cctgcttccg tcacaatacc aaaagcatgt tttgttggga agatgcttga cacagtacct
    45961 gaaactggag atacaatgtt tccatttgca ggttctactg caaatccatc acccatcatt
    46021 ttttgagcaa atactggatc ctttacttgt tccaaagcaa caacttgacc gtctgctact
    46081 gagtaaactt cctcagtaag atctttgaag tgaacagtgt tttgttgtgc ttcagtcatt
    46141 tggcttggaa gagtttcagg aatgatttca cctgaatcaa ggatatcttg gatatcagat
    46201 ttcaaaatgt cagcttttgg accgtagata gcttgaaccc cttgtccttt catgacaaga
    46261 cccatagctc cttctgcttt ccattgctct gcatttccta ctttatctgc atctttaaca
    46321 gttacacgaa gacgagtcat acatgcatca acatcaacga tgtttacacg tccaccaaga
    46381 aggttgataa tgtttacagc ttgagagcct gctgcaactt tcacttcgct gctggtttct
    46441 tctgaacctt cagcagtttc gtagtttccg ttgcgccctg gagttgcgta gttgaatttt
    46501 tgaatcatga agtttgcgat aaagtacatg attacagcaa agagaacagt tacccaaacg
    46561 aagttaacga tatccatacc aataccagca ctgattgcaa taggtgtacg agtcaagaac
    46621 tcgattgaac cgaatgagtg catacgtagg tttacgacgt cagccatagc gaaggcagca
    46681 ccttgaacaa gtgagtaaac aagatacata ggtgttgcga tgaacatgaa catgtattcg
    46741 attggttcag taacccctgt caagaatgtt gcaagagctg ttgcaatcat catacctttg
    46801 tatttatgtt tcttgtcagc atcaacatta cggtagatag caacaatcac acccatcaag
    46861 ataccgaatg aaccgatcat ttgtccaact ttgaaacgag ctggatgtac tgtatctaac
    46921 aagtgttgat attgactagc atcagtacct ttaaggttta caaggtctgt tacccatgca
    46981 agccatagtg ggtcttgacc gaatacttga gtacctttag ctgcaccagt taaaatgtca
    47041 taagtaccac caagagctgt gtagttcatt gggatagtca acatgtggtg aagaccaaat
    47101 ggcaagagca aacgttccaa agtaccatac aagaatggtg caagaattgg agcagtttct
    47161 tgtgagttgg caatccagat accgaagtta ttgatacctg tttgaactac tggccagaaa
    47221 gcagcaagta gaattgcagc gattgctgaa cgaagaataa ctacaaatgg tacgaaacgt
    47281 ttcccgttga agaatgaaag tgcatcagga agtttacgga agttgtagta tttgttgtaa
    47341 gcagttgccc ctacaaaacc tgagataatc cctacgaata cccccatgtt caaggctgga
    47401 gcttcaagaa cactgataaa gtaatcagca actttgattg aaccaccaaa gaaagtagtt
    47461 accatagcat ctggattttt caacatatcg cctgatacac caaagattgt accagtgata
    47521 cggttaatca agatgaaggc aagaccagcg gcgaaagcac caccagcacg ttctttagcc
    47581 cagcttcctc caatggctag ggcaaacaaa atgtgaaggt taccgataac cccccaaccg
    47641 atttgctcaa gaattccacc tgtgatgaca agtggtgcaa aggttgggtt aatcatcacg
    47701 atagacttac cgattgaaat catcaaacca gcagccggca taaccgcgat aactaccatc
    47761 aaagccttac cgaatttttg ccaaaattcg aaagacaaga catttttgaa tgtatctttc
    47821 atcattcaaa tctcctttat ttttatttag gcaaacgttt tacgaaaacg tttgcacttt
    47881 ctatgattta attataccac tttaattttt tttgtaaagg cttttataaa aaaattacac
    47941 ttttttctaa aaaatttcta gtgcgccaaa tttcaagttc aaattagcca tcaagaagtc
    48001 ttctctgggg gacttctttg gaagctcttt tcttctgcgc atctcttttc aaaaactctt
    48061 gtagcaaaaa aggagatttt acaatctcct cataaaagca gctattttac ctaaatcttc
    48121 aagaatcggc gcatggatat tcctgatccc aatgaaccaa tgaaaacccc aatcacaaat
    48181 agtagggcaa tcatcaacgg actaaataaa tctggactaa tcatggatag attttgccct
    48241 accaacgatt tgttgacaga ttggtaaaca atttgataaa caataaagac caaaacagat
    48301 ggtgcgatag ctcccaataa accgataaag gctccttcta acaagaacgg tccacggata
    48361 taactgtttt tagctccgac caagcgcatg atttgaattt cgcgactgcg ggaaataatg
    48421 gtaatacgaa tggtatttga aatcaagaaa actgcgataa aaattaacaa agcagcaatc
    48481 cctagtcccc aaacacggat aaatgaagct aacttgaaga gtctttctgt attggcaccg
    48541 ccatcttgaa cctcagagac accttcaatt tttttagcat cttcggctat agtttttaca
    48601 tcatttggag tgtttgcctc tacaatatag gcatcataga gaggattggc atctccttca
    48661 aagattttcc agttatctcc cattatctcg gttaattttt catattgttc ttctttactt
    48721 gaaaaggtaa cacttttaac cgtagacatg ttcttcaaag aatcatatac cttgtggtag
    48781 tcattatttg taacagtttg accttctttt tcaattgtct gactattatc ttccacatcc
    48841 tttcggatat aaactactac acggacatta ttttcaatat ctgtagctag tttcgctgta
    48901 ttgaaaataa cagatgcaaa tattgccacc aaggtcaaag taatcatgac tgaactgaca
    48961 gcagctactg tcatccaacc atttcgtttc aaacttttta aggcttcaaa taaatggcga
    49021 aaaaatctac taatcatcgt atccatactc tccttttgat tcgtcacgaa cgacacggcc
    49081 attttcaatg gcaatgacac ggtggcgcaa ggtatttaca atctggctat tatgagtcgc
    49141 catcaaaata gttgttcctt gtaggttaat ccgttccaag agattcataa tttcccatga
    49201 attatccgga tccagatttc ctgttggctc atcagctatc aatactttgg gattatttac
    49261 aattgcacgc gcaatcgcaa tccgctgttg ctccccacct gagagttcat ttgggaaaga
    49321 acgaacctta tgcttcaatc caaccaagtc caaaacttcc atcactcgtc ttttgatatt
    49381 acggcgattt tccccgatta cttccatagc gtaagcaata ttttcataga cagttttctt
    49441 tggtaacaat ttataatcct ggaagacaac cccaacacta cgacgtagaa gcgggacatc
    49501 tttctttttg atcttaacca gattaaaacc agcaactgat aggcttcctt tatcgatttt
    49561 tacttcacga tacagagaac gaataaaagt tgacttccct gctcctgaag gtcctacgat
    49621 gtaagcaaat tcccccggtt gaacgctaac cgaaacaccg cgtagagcag ttgttccgtt
    49681 gtcgtatttt ttaacgacat ctctcatttc aataattgac atgtgagttc ctttctatct
    49741 tagctaattc gccacttgag ataagcatcg ataaaaccat ctaggtcccc atccataacc
    49801 ttatctacct gagcaacctc aaagctagtt cggtgatctt ttaccatagt ataaggcgtg
    49861 aagacataag aacggatttg gcttccccaa gtgatctcct ttttctcacc tttgagagaa
    49921 tctacctccg cagccttctt atcttgctcc atttgataga gcttagcctg caacatcttc
    49981 atggcacgat ctctatttcc atactgggta cgatctactg ttgattggac aacaattcca
    50041 gttggaatgt gggttaaacg tacacctgtt gaaaccttat tgacgttttg tccaccggca
    50101 ccacctgaac ggaaggtatc catcttgata tcatcttcac ggatttccac ttcaatagta
    50161 tcatccaatt ctggcatcac ttctacagat gtgaaagagg tatggcgacg tttggcagag
    50221 tcaaatggtg agattcgcac taagcggtga acacccattt ctgacttgag gagaccatag
    50281 gcattaggcc cttcaaatga taaagttacc gacttaatac cagcctcatc acctgcttgg
    50341 taatccaaca cttccacttt aaagccttta gcattaccat aacgagtata catacgaagc
    50401 aacatatcac cccagtcctg cgcctcagta ccaccagaac ctggatggat ttccaagatg
    50461 gcattgttgt ggtcataagg ttctgacaag agtagagtca tctcgtagct ggtcattatc
    50521 ttatcaagtt cggctaactg cgctaccagt tcatcatgca ctgactcgtc ttcagccaaa
    50581 aaatccaata aaatttcgac ttcatcctgc aactcttcca tcttatggaa ggtattgtaa
    50641 gtgtttttta attcatttaa ttcttgcgac gttttttggg ccgcaatatt atcgttccaa
    50701 aaatcaggtt ctgtcatctt gttttccaag atggcaatct cttcctctag cccttcgagg
    50761 tcaaagagac cccctgaaag aagctaattt ttcacgattt gcgtcaattt tctgacgaat
    50821 tactgaaatg tccataaata ctcctttttt acattatttc attatagcat attttatcat
    50881 ttctttccat cttttgctct aatccccttt tcatgaaaaa agaagccttt gggcttccct
    50941 tcttacaaga cttttgctga agtgcgagcg atttcttcca cttgaatacc atttgcttca
    51001 aatttttctt tcaaggctgg taaatcaatt gatccatcga tttgcacttc gataatcacc
    51061 ttaccatcct tacgcggaat attgactgta tgggagatat tcaaattttc ttctacaatc
    51121 aaagaaacaa tttttccaag aacaccaact tcatcttctg taacaaagcg tacacgaatc
    51181 ccttcttcgc cataacctgc aatttcaaga aaggcttgga aaacgtcacg gtcagtaata
    51241 actccgtata cttgatggtt atctacgaca gggagaatac taatcttatt tttcaacatc
    51301 agataagttg catcttctag actagcatag cctgagacag tgacaacatc gcgaatcatg
    51361 acatctttta cttttgtctt attcagaaga taattcatct catagataga aagacttgtt
    51421 gctttagatg gacttgcttg tgcaatggtt ccctcagtca ccaaaccaac taattgatca
    51481 ttttcgataa caggcagacg gtgcaaacct tgctctctca tcaaatctgc tgcatgagat
    51541 actgttgtat ctggactaat ataaactacc ttgcgggtca taaaatcttt aactgccatg
    51601 agacttctcc ttttctattc ttatccctat tatacaatct ttttgtaaat ctatcaaacg
    51661 cttacatttc tttttcgaaa ttcagaaaag tatgaataag ctagcaaaaa gagctctgaa
    51721 atcgagctct gtgaatgaag gtaagaaatt tttcattcga ttttttcaat tattagaaat
    51781 taagctttca atgtagatgc agatttgttt gcaatctcaa tctaccgact aaaaaggtcc
    51841 cccggactat attgatgatt acttcgtaat ctccatctgc cgactaaaac aatccactgg
    51901 attgttttag ccacctagat atgcttttct gacttcttct gatgaagcga gttcttttcc
    51961 tgttcctgat aggacgattc tccctgtttc cagtacatat cctcggtcag agattgcaag
    52021 tgctttattg gcattttgtt caatcaagag gaccgttgtt ccttgcttct gaatatcttg
    52081 aatgatatca aaaatttctt ggataaagat tggggcaagt cccattgatg gttcatctaa
    52141 aagaagaagt tttggtgttg acatgagggc gcgtcccatg gcaagcattt gttgttcccc
    52201 ccctgaaaga gtggctgcat cttggttctt ccgttcttca agacgaggaa agcgtgagaa
    52261 aaccttcttc aagttagctt gattttcttc acgatttttc tttaagaaag ctcccatttc
    52321 aagattttcc ataacagtca agccaggaaa gacgtggcgt ccttctggaa cttgtgaaag
    52381 accacttgcc acgattttct gagctggcat tttttggatt tcttgaccta aaaattcaat
    52441 ctttcctgaa cttggtcgaa ccaaacctga caaggtgcga agaatagttg tcttacctgc
    52501 accgttggca ccgataaggg aaacaacttc tccttcatta acttcaaagc ttacatcacg
    52561 aactgcttgg atcataccgt aatgcacaga aagattttca acttttaaca tagacattag
    52621 gcttcacctc ctagataagc ttcgataacg cgtttattgg tcttaatttc gtctggagtt
    52681 ccttgagcga ttaaacggcc atattcaagt acgtagatac gttctgttac ttccatgacc
    52741 agattcatat cgtgttcaat caacatgatt gtaatcttaa actcatcttt gatacgacga
    52801 attaactcag tcaattcggc tgtttcctgt gggttcatac ctgctgctgg ttcatctaag
    52861 aagagaattt taggttccgt agcaagggca cgaacaattt ccaaacgacg ttgttgtccg
    52921 taggaaagat ttttagcaag agtctctgca tcaccatcta aatcaaagat tttcaacaat
    52981 tccaaagctt tagcctttaa ttctttttca ctcttgtaaa aagctggtaa gcgtaagaaa
    53041 ctagtaaaaa catgctgttt gtgatggttt ccaaaagcaa tcaaaacatt atctaaaact
    53101 gttaaatctt taaagagacg gatattttgg aaagtacgtc caagtcccaa agaggcaatc
    53161 ttataaggtg atttcccatt caaaaggtga ccatctaggg ttactgttcc ctcgcttggt
    53221 tcataaacac cggtcaaaag gttgaaaagg gtggttttcc cagctccgtt tggaccgatt
    53281 aatccaacca gttccccttc gttcaattca agagtcacat ctccaacagc tgttagacca
    53341 ccaaaatgtt tggttaactg ttttacttca agtaatgcca ttagttttgt tcctccttct
    53401 tagatttttt aaagaaacgt gataggctca gttcccatgt tccaaggagt ccacctggtc
    53461 tgaaaatcat taccaatacc aaggccaaag cgtaaataat catacgcaca ctagcaacat
    53521 cttggagaag catattcaaa attcccagaa caatagccga aacaatcgca cctgtaatgg
    53581 aaccgagtcc accaaataca acaataatca aaacgttgat tgagttgatg aaggtgtaat
    53641 ctttcggtac aacagacccg ataaatcctg cctgaagtga cccagcaata cttgcagtaa
    53701 tggcaccaaa gacaaaagcg atgattttaa ttttagtcgt attaacccca actgactcag
    53761 cagcgatttc atcttcacga acagagaggg ttgaacgacc aattgggcta cgcaagaagt
    53821 tcaaggttgc aatggttgta atcacgacaa agaagtaaac catttgccaa gttgtaaagt
    53881 taggaatccc taagatacct gccgcaccat ttgtaaggct tccaccattg atgataaaga
    53941 tacggataat ttcagaaaca cccagagttg ctaccgcaag atagtccccc ttcaagcgca
    54001 aggttggaat gccgacaagt aaggcaactg ctcctgaaag caaagcccct acaagcatag
    54061 ctccaaagaa ggcaccgtag gttggtgatt tagaaccaat aatagctgct gcataggcac
    54121 caatcgccat gaaaccagca tgaccaagtg aaaattgtcc tgaaaaacca acgattaagt
    54181 tgagaccaac agccaaaata atattaattc caatttgttg taaaatctgt acatagaata
    54241 gattaagtac tccgactgaa accagtacac taatcaagct atagccagct aacaaaagga
    54301 gtaaccatag aatattaact tttaaatttt ccttcatcgt ttacaccttc tctttcacat
    54361 tcttaccaag gataccagct gggcggacaa tcaagatcaa caacaagatt ccataaacaa
    54421 tggcatcacg gaaatctgac atcccaaagg cagtcgcaaa ggtttccaat agaccaatca
    54481 caaagccacc aagagccgca ccaggaataa ttccgatacc accaagtact gcggcaacga
    54541 aagatttaag acctggagta acccccatca aaggctcaag agagttataa taaagagcaa
    54601 tcagaacacc agccgcaccc gcaagagcag aacccaaagc gaaggtaaag ctaatcgtac
    54661 ggtttacatt gatccccatc aattgcgccg cgtcgctatc tactgatact gcacgcatgg
    54721 ctttccccat cttagtcttt tggacaatga cttgtaacaa aatcatcaaa atcaaggaaa
    54781 tggccaaaat cattaactgc acatttgtta agctaattgg tcccaaatca tatcgaactg
    54841 tttgaatcgc ttgagggaag gcacgggtat tggcaccaac cagatagacc attccatact
    54901 ccaataggaa agaaacccca atagccgtaa tcaaaacagc aatacgagta gagtggcgca
    54961 aaggtcggta agcaagaaac tcaatcacga caccaagaat agctgtcgct agcatagcta
    55021 caataagcgc tacaaagaaa ttcatttgga aagaattgat caagaaataa ccgataaagg
    55081 ctcccatcat ataaatatca ccatgggcga agttgatgag cttgataatt ccgtaaacca
    55141 tggtatatcc tagggctaac agcgcgtaaa cactacctag aatcaaacca tttacgagtt
    55201 gttggagcat aagattcact ctttctattt ataattccga gggttttccc tcactttttg
    55261 ataggttctt atactcaatg aaaatcaaag agcaaactag gaaactagcc gcaggttgct
    55321 caaagcactg ctttgaggtt gtagataaga ctgacgaagt cagtcacata tataatccaa
    55381 ggcgacgttg acgcagtttg aagagatttt cgaagagtat taaatatcga aacagggagt
    55441 gagtcaaagg ctcattccct atttcaacat ttttctatta tggttttaca acttctgctg
    55501 cttcaacttt accattgttc atggtcatca tgtaagcagt tttgactgtg ttgtggtctg
    55561 catcgaagct tgtttgacca gttacacctt caaaatcttt tgttttagca aggttattct
    55621 tgatttcacc tgaattttta gcaccttttg ctgcgtttgc tacaaggtga actgaatcat
    55681 aagccaaggc tgcaaatgtt gaaggctctt cattgtactt agcacggtaa gcgtcaagga
    55741 aggctttagc tttagctgaa acttctacag tagttgagaa gcctgagata aagtagatgt
    55801 ttgatgcttt ttcagcagtt gcttgttgta caaactcctc accgttgaat ccatcaccac
    55861 caacgattgg tttgtcaatt cccatgccac gcgcttggtt tacaatttta ccagcctcat
    55921 tatagtaacc aggaacaacg atagcatcaa agtctttccc tttcattttt gtaagggctg
    55981 cttggaagtc tgtgtcacct gctacgaaag tttcatctgc aacgatttca cccttgtatg
    56041 actcgcggaa agattttgca atccctttag catagtcact ggcattgtca gtgtaaagaa
    56101 caactttctt agcatttaat ttttcagaaa catagtttga gataattttt ccttggaagc
    56161 tatcttggaa agttccaata aagaggtaat cttgaccttt agtcaatcca tcttgagtcg
    56221 cacttggtga gatcaatgga acacctgctt ttgtagcgtt cgctaccgca gctgcagtcg
    56281 caccagatgt cgcaggtcct acgactgctg atactttaga ttgggttaca aggttagttg
    56341 taactgaagc agcctcagct gtttcagact tattatcttt atcgactact tcgatttgtt
    56401 ttccatcgat accacctgcg gcattgattt catcaacagc caattgggca cctttttgtt
    56461 cagctgttcc gtatgcagct aaagaacctg attcttcaaa gttaaacccg attttaattg
    56521 tcttttcctc tactgagtta ccagcagtgt tgactgctcc agacttcact tctccacagg
    56581 ctgcaagaag tgctacactt gcaagcgcca caaacgatag ggcaaatttt ttcttcatct
    56641 ttttgtctcc ttatttctta aatagtctgt taagaatata ccgaattatc agaaaattgt
    56701 caatgttttc cttgaacttt ttcgatgata acgttttcct atcgataaag attgcccaca
    56761 aagggtgttt ccaactcttg gatatgacaa accctaactt ttttaataaa tttttccttg
    56821 ctcaagcgtc cgaccaattg ctccacttct tgagttggaa catagagttg taagtaacga
    56881 tgtttcttgg aatgataggt aatatctcca taatcctgca gttttttggc atcacgatta
    56941 tagtaaagat agataatcaa gccagagcga ttgacttttt caaacatgat aaatcctctt
    57001 tctaagaaat ctctccctat tataccaaaa aaagcaaacc cagtcgagtt tgccttctat
    57061 catcattagt tcaatgaatt gttggccata atttcatcaa taaagccata ttcaagtgtt
    57121 tcctgggcgc tcatccagtt atcacgttct gcatctgcat ggactttttc cattgactga
    57181 cctgaatttt cagccaagat tttttccaag gtattacgag ttttgagcaa gtgttctgca
    57241 gcgatagcca tatcagtttg ttgggtacca ccacctgtac cacccattgg ttggtgaatc
    57301 atgtattcag catttggaag catgaaacgt ttgccttttg ctccacttga tgcgatgaca
    57361 gtccccatag atgcagccat tcccataaca atggtttgga catctgcctt gataaagttc
    57421 atggtatcta cgattgccaa accagctgaa acagaaccac ctggtgtatt gacataaagg
    57481 taaatatctt ttgtactatc ttgggcatcc aagaaaagca attgggcaat aacagagtta
    57541 gccatattgt cttcaaccgg acctgtcagc ataatgatgc ggtctttgag aagacgtgag
    57601 taaatatcgt aagaacgttc tccacggctt gtttgttcaa taactacagg aatcattcat
    57661 ttctcctttt gagttttaat tttgttggtc aaatgactga agataagact attataatat
    57721 cttggtcaaa aaaggtcaaa tttttgctct gctttcatta gacagaaaca aaaacccaac
    57781 ctcctttcgt gactggaaat acttttccaa gtcattcttc ttttcgatct tattttgtac
    57841 cgaacaagcg gtctccagca tctccaagac ctggaacgat ataaccgtgt tcgttcaaac
    57901 gttcatccaa ggctgctgta aagatttcta catctggatg agcttcttga agggctttta
    57961 caccctctgg agcagataca aggcagacaa atttgatatt tgatgcgcca cgttttttaa
    58021 gagaatcaac agccaagatt gctgagccac ctgttgccaa cattgggtct actacaaaaa
    58081 tttgacgttg gtcaatgtcc tcaggcaatt tcaccaagta ttcaactggt tgaagtgttt
    58141 cttcatcacg gtacataccg atgtggccaa ctttagcagc tggaaccaag ttcaagagac
    58201 catcaaccat cccgatacct gcacgcaaga ttgggacgat ggccaatttc ttacctgcca
    58261 attgtttttg aactgttttt gtaattggtg tttcgatttc cacatcttct agtggaagat
    58321 cacgaagtac ttcatacccc atcaacattg caatctcatc tactagctca cgaaaagctt
    58381 ttgtagaagt atctgtacga cgcaagattg acaatttgtg ttgaatcagt gggtgattaa
    58441 taacttcaat ttttcccatt tttggaattc cttctttcaa tttattcttc ttattatacc
    58501 aaaaaacggt ttaaaaatct ttctaaacca tttatttttg ataattttta cattagatca
    58561 gcctctttaa gagctgtctg tactgtctca agtggtaaat gggtcaattc tgtccctttt
    58621 tcttgataaa ggtattgggc gtagtcgtcc attcggtact ggttgatata aaccacgcgc
    58681 ttgcagccga cctgaagcaa ttgttttgta cagttgagac aaggaaaatg ggttacatag
    58741 gctgtaaagc ctttgggaac accacgctca gcaccttgaa ggatagcatt gacctcagcg
    58801 tgaagggtgc gaacgcagtg gccttcaatt accaaacatt cgtgatcaat acaatgctca
    58861 gtccctgaca ccgaaccatt gtaaccagtg gaaataacct tattatcttt taccagaatc
    58921 gcgcccactt tagcacgttt acaagtggaa cgattcgcaa ttagtagagc ttgggctgca
    58981 aaatactcat cccaggccag tcttttttca gtcatctctt ttctcctttt tctctatttt
    59041 ttaaaaaatg gtaaacctaa atctgcaatc ttttcagctg gtaccttcat gccatccttg
    59101 atccatttta gaaggacaga gacgatggct gagctccaga aggaatgaag ataagagctg
    59161 acaccttttg atttcccatg gtatttttct agaaattcct gcatggcttg gacaaagatt
    59221 ttttccagat ggtaatccaa ggccaattga attactctag cttcctttct ggcctcccgg
    59281 aaaaggtgaa cccaaaccaa ataaaggtct gtctttaaat cgtaatgatg cagctgttcc
    59341 ataatattgt ggacagttcg tttaaagacg ctctctaaaa tttcctcttt ggagtcataa
    59401 ttgcgataaa aggccgcacg cgaaacacct gcacgtttga ccaattcaga aatactaatc
    59461 ttggtcagtt cctttttttc caagagttgc aagagggctg tttcaatggc ttctctggtt
    59521 aataaattgg attcttggtt tgattttctg agattttcaa gagacttttc agagattcta
    59581 cgttcagaca taacattttc tttctacttg tcacaacaga cggatgatgc ttttgtttca
    59641 agggttttca tgatataatt gtaaaaaaag acagaacctt tgtcaatgta taactgacaa
    59701 agatggagaa tttctatgac ttggaagatt attgctgact ctggttgtga ttatcgtcaa
    59761 ctgccaacac cagctattaa cacaaccttt gtaagtgtcc ccttaaccat tcaagtagct
    59821 gatcaggtct ttgttgatga cgccagtctc gatattgacc aaatgatgga aaccatgtat
    59881 gcaactgcag aagcttcaaa atcggcttgt ccaagcccag atgactattt gcgagcattt
    59941 gaaggtgcca aaaacatttt cctagtaacc atcacaggta ccctttctgg cagtcacaat
    60001 agtgctcaac tagcaaagaa tatttatctg gaagaccatc ctgacactaa gattcatgtg
    60061 attgatagtt tatctgctgg tggtgaagtt gacctactcg tagaaaaatt gaatgacttg
    60121 attgaccagg gcttgtcttt tgaagaagtg gttgaagcta tcaccgccta tcaagaaaaa
    60181 actaagttgc tctttgtcct agccaaagtc gataacttgg tgaagaacgg ccgtttgagc
    60241 aagcttatcg gtacggtcgt tggccttctc aacattcgta tggtcggaaa agctagtgaa
    60301 actgggactc tcgaattgct acaaaaagca aggggatcaa agaaatcagt tcaagctgcc
    60361 tatgatgagt tagtaaaagc tggatatgct ggtggccgta ttgtcatggc ccaacgcaat
    60421 aacgaaaaat gttgtcaaca gctctcagag cgaatccgtg aaaccttccc acaagcggat
    60481 attaaaattc taccaacctc tggtctctgc agtttctatg cagaagaggg cggtttgctg
    60541 atgggctatg aaattgatta attgcattct tgacaagagg tgaccatcat ctcttgtttt
    60601 tttgtggtac aatagagcta tgaaacattt tgatactatt gtcatcggtg ggggacctgc
    60661 tggtatgatg gctacgattt ccagtagctt ttatggacag aaaaccctcc tcatcgaaaa
    60721 aaatcggaaa cttggaaaaa aattagctgg gactggtggg ggacgttgca atgtgaccaa
    60781 caatggtagc ttagacaacc tgctagctgg aattcctgga aacggacgct ttctttacag
    60841 tgttttctcc cagttcgata atcatgacat catcaacttt tttacagaaa atggtgttaa
    60901 acttaaggtc gaagaccacg gacgcgtctt tccagccagt gacaagtctc ggactattat
    60961 cgaagctttg gaaaagaaaa tcaccgaact aggtggtcaa gttgctactc aaatagaaat
    61021 cgtttctgtt aaaaaagtag atgaccagtt tgtccttaag tcagcggatc aaaccttcac
    61081 ttgtgagaaa ctcattgtca caacaggtgg taagtcttat ccttcgactg gttcgactgg
    61141 ttttggtcac gagattgctc gccattttaa gcataccatc accgatcttg aggctgctga
    61201 aagtccttta ttaacagatt ttccacataa agccttacaa ggtatttctc tggacgatgt
    61261 gaccctaagt tatggtaagc atgtcatcac tcatgattta ctctttaccc actttggttt
    61321 gtcaggtcct gctgccctac gcatgtctag ctttgtcaaa ggtggggagg ttctctcact
    61381 cgatgttttg cctcaacttt ctgagaagga cttggttaca tttctagaag aaaatcggga
    61441 aaaatccttg aaaaacgctt taaaaacctt gttaccagaa cgcttggccg aattttttgt
    61501 acaaggatat cctgaaaaag tcaaacaact gactgaaaag gaacgagaac aacttgtcca
    61561 gtccattaaa gaacttaaaa ttcctgtaac tggaaaaatg tcccttgcaa agtcctttgt
    61621 taccaagggt ggagtcagtc tcaaggaaat caatcctaaa acccttgaaa gtaagctggt
    61681 acctggcctc cactttgcag gcgaagttat ggatatcaat gcccacacgg gtggctttaa
    61741 catcacttct gccctctgta ccggctgggt ggcgggaagt ctgcattatg attaaagatt
    61801 aaaggttgtg aagaatgaac agaagagagg cagtcgaatt tgttaacatg tgtatgatta
    61861 aaaacggaga taaggtcctg gtccaagacc gagttaatcc cgactggtct ggcattactt
    61921 ttcctggtgg tcatgttgaa cgtggcgaat cctttgtcga tgctgtcatt cgtgaagtga
    61981 aagaagaaac tggtctgatc atttccaaac cccaactctg tggtatcaaa aactggtatg
    62041 acgacaagga ttatcgttat gtcgtccttt tttacaagac agaacacttt actggtgaac
    62101 tccagtcttc agacgaaggg aaagtttggt gggaggactt tgaaaatctt tctcatctaa
    62161 aacttgcaac cgatgatatg tctgatatgc ttcgtgtgtt tctagaagaa gatctcagtg
    62221 aattctttta ctacaaaaac ggtgacgact ggctttatga tttgaagtaa aaaataggct
    62281 aggaaattcc tagcctattt attctgcatt tgtctgaacg taaaccgcaa tggcatctga
    62341 cgtgtactgg gctgtttcag aaccaaactt gtcaatgttt tccttaaact ctgggttgta
    62401 gacgtaacct ttaccgatat gaccgaatac ctcaatagag cagtcaaatc cataagtgcg
    62461 aatggcttgc aagagcttgg ctgcttgctc ctggttttcg gttgctgttg caggtaaacc
    62521 aacttgaaga ttttgtgcca aagtttgaaa gacttggtta aaggcggccg tagcctcgtc
    62581 ttcgtgacct ttttggcgtt cgagcgcttg tcccatgact tcttgaccat atttctctac
    62641 cgcttcttgg tggtattttt gattgtcttg atagctaaat cccgtgaatt tttcctcaat
    62701 ggtcattttt ctttctcctt tttgttcttg aatagttttt tgcaaggtgg aaatcaaggt
    62761 atccagatgt tgcctttcgc gagttagata gtccaactgc ctagtcaaat ggggcaataa
    62821 atctgtcctt tcttccttta acagctctgc tattttctct aaagaaaagc ctagatattt
    62881 gtagtaaaga atgacctgaa ggcgttccaa atcctcttga ctgtaggttc gatagccgtt
    62941 ttccgacttt aaggggacca agagtcctat cttgtcatag tgatgcaggg tcttgacaga
    63001 gacacctgaa agctgcgcag cttcttttat atggtacatg atttcctcct tacaatgata
    63061 gtataacccc tcgccttagg tcagagtcaa gcgtttttga gaaaattttt taactttttt
    63121 gagaaaggtg tgaaaacttg aaaatgattt tcttgacaga ccttagaaaa catgatagga
    63181 tagtcaagtc tatcaatcaa acagaaggct atttgacaac tcaagtagct ttttcttatt
    63241 ttgaaaaagg agatcagagt ttaactatgt cagaaaaatc acaatggggg tcgaaacttg
    63301 gttttattct agcatctgct ggctggccat cgggcttggt tccgtttgga agtttcccta
    63361 catgactgct gctaatggcg gtggaggctt tttactaatc tttctcattt ccactatttt
    63421 aatcggtttc cctctcctgc tggctgagtt tgcccttggc cgtagtgctg gcgtttccgc
    63481 tatcaaaacc tttggaaaac tgggcaagaa taacaagtac aactttatcg gttggattgg
    63541 cgcctttgcc ctctttatcc tcttatcttt ttacagtgtt atcggaggat ggattctagt
    63601 ctatctaggt attgagtttg ggaaattgtt ccaacttggt ggaacgggtg attatgctca
    63661 gttatttact tcaatcattt caaatccagc cattgcccta ggagctcaag cggcctttat
    63721 cctattgaat atcttcattg tatcacgtgg ggttcaaaaa gggattgaaa gagcttcgaa
    63781 agtcatgatg cccctgctct ttatcgtctt tgtttttatc atcggtcgct ctctcagttt
    63841 gccaaatgcc atggaagggg ttctttactt cctcaaacca gacttttcaa aactgactag
    63901 cactggtctc ctctatgctc tgggacaatc tttctttgcc ctctcactag gggttacagt
    63961 catgttgacc tatgcttctt acttagacaa gaaaaccaat ctagtccagt caggaatctc
    64021 catcgtagcc atgaatatct cgatatccat catggcaggt ctagccattt tccaagctcg
    64081 atcccccttc aatatccagt ctgaaggggg acccagcctg ctctttatcg tcttgcctca
    64141 actctttgac aagatgcctt ttggaaccat tttctacgtc ctcttcctct tgctcttcct
    64201 ttttgcgaca gtcacttttt ctgtcgtgat gctggaaatc aatgtagaca atatcaccaa
    64261 ccaggataac agcaaacgtg ccaaatggag tgttatttta ggaattttga cctttgtctt
    64321 tggcattcct tcagccctat cttacggtgt catggcggat gttcacattt ttggtaagac
    64381 cttctttgac gctatggact tcttggtttc caatctcctc atgccatttg gagctctcta
    64441 cctttcactt tttacaggct atatctttaa aaaggctctt gcaatggagg aactccatct
    64501 cgatgaaaga gcatggaaac aaggactgtt ccaagtctgg ctcttccttc ttcgtttctt
    64561 cgtttcgtca ttccaatcat catcattgtg gtcttcattg cccaatttat gtaatcaaaa
    64621 aggacttgag tagtgaactc aggccctttc tttttatgga tggctaacaa tcaattccaa
    64681 accttgccct tccagagtcc aagcttcaac atcacttggt aggataaagt ggctgccttt
    64741 ttgaattgga taatttttcc cgtcaacagt tagctgacct tgaccagcca agacactcaa
    64801 taagctgtag tcagctgtct tttcaaagtc aacttttcca gtaatttccc acttgtaaac
    64861 tgcgaagaaa tcattagata caaggagagt ggaacgcaaa tcatctgctt taacagttac
    64921 aggacggcta tttgctggct caccaatgtt caagacatcg atggattttt caagatgaag
    64981 ttcacgcaag ttgcctttgt catccttgcg gtcaaagtca tagacgcgat aggtggtatc
    65041 gctagactgc tgggtttcaa ggattaagat acccgccccg atagcgtgca tagtcccgct
    65101 tggtacatag aagaaatctc cagccttaac agggactttg gtcaacaagt catcccagtt
    65161 cttgtcctcg atttgctggc ggagttcttc ttttgacttg gcattgtgac cgtagataat
    65221 ctctgaacct tcatccgctg cgataatgta ccagcattct gtttttccga gttcgccttc
    65281 atgctcgagt ccataagcat cgtctgggtg aacttggaca ctgagccagt cgttggcatc
    65341 gaggatcttg gtcaaaagtg gaaatacagg ttctggacga ttgccaaata attcacggtg
    65401 ttccgcatac aaagtagcaa gatctgttcc ctcgtaacga ccattggcaa ctttagagac
    65461 tccatttgga tgggctgaga tggcccaata ttctccgatt ttttcacttg ggatgtcgta
    65521 gccaaactca tcacgtagct tggctccacc ccagattttt tcttgcataa ctgattgtaa
    65581 aaataatggt tctgacatgt cgatctcctg tctgattttt ctcccctcat tatagcaaaa
    65641 aaagagttcg aattgaactc ttttttacat cttataaagc agggagaaga ttttataaaa
    65701 atagtaaaca aatgtgctct acccgatgct tgcaccattg ctataaatga catccttgta
    65761 ccaatagaag gacttcttct tgctacgttt gagagctccg tttcctacat tatctcgatc
    65821 tacatagata aagccatagc gcttattcat ttcccctgtg ccagctgaaa ccggatcgat
    65881 acagccccaa gtcgtataac caagcaagtc aaccccgtct tggtaaatgg catctcgcat
    65941 ggccttgatg tgggcctcta agtaagtaat ccgatagtca tctgctacat aaccattctc
    66001 atccggtgta tccatagcac cgagtccatt ttctacgata atactaaact aaaatcaaaa
    66061 agcattatat aatagtgata tgaaa
//
LOCUS       DAVHEX010000004        63873 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVHEX010000004 DAVHEX010000000
VERSION     DAVHEX010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 63873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63873
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.004"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            2..74
                     /locus_tag="VVL45_000234"
     tRNA            2..74
                     /locus_tag="VVL45_000234"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            99..171
                     /locus_tag="VVL45_000235"
     tRNA            99..171
                     /locus_tag="VVL45_000235"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:132..134,aa:Lys,seq:ttt)
     gene            177..258
                     /locus_tag="VVL45_000236"
     tRNA            177..258
                     /locus_tag="VVL45_000236"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:211..213,aa:Leu,seq:tag)
     gene            277..349
                     /locus_tag="VVL45_000237"
     tRNA            277..349
                     /locus_tag="VVL45_000237"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:310..312,aa:Thr,seq:tgt)
     gene            372..443
                     /locus_tag="VVL45_000238"
     tRNA            372..443
                     /locus_tag="VVL45_000238"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:404..406,aa:Gly,seq:gcc)
     gene            451..536
                     /locus_tag="VVL45_000239"
     tRNA            451..536
                     /locus_tag="VVL45_000239"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:485..487,aa:Leu,seq:taa)
     gene            546..619
                     /locus_tag="VVL45_000240"
     tRNA            546..619
                     /locus_tag="VVL45_000240"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:580..582,aa:Arg,seq:acg)
     gene            635..708
                     /locus_tag="VVL45_000241"
     tRNA            635..708
                     /locus_tag="VVL45_000241"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:669..671,aa:Pro,seq:tgg)
     gene            714..787
                     /locus_tag="VVL45_000242"
     tRNA            714..787
                     /locus_tag="VVL45_000242"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:748..750,aa:Met,seq:cat)
     gene            801..874
                     /locus_tag="VVL45_000243"
     tRNA            801..874
                     /locus_tag="VVL45_000243"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:835..837,aa:Ile,seq:cat)
     gene            885..974
                     /locus_tag="VVL45_000244"
     tRNA            885..974
                     /locus_tag="VVL45_000244"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:921..923,aa:Ser,seq:tga)
     gene            988..1061
                     /locus_tag="VVL45_000245"
     tRNA            988..1061
                     /locus_tag="VVL45_000245"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1022..1024,aa:Met,seq:cat)
     gene            1065..1137
                     /locus_tag="VVL45_000246"
     tRNA            1065..1137
                     /locus_tag="VVL45_000246"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1098..1100,aa:Phe,seq:gaa)
     gene            1157..1227
                     /locus_tag="VVL45_000247"
     tRNA            1157..1227
                     /locus_tag="VVL45_000247"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1189..1191,aa:Gly,seq:tcc)
     gene            1263..1336
                     /locus_tag="VVL45_000248"
     tRNA            1263..1336
                     /locus_tag="VVL45_000248"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1297..1299,aa:Ile,seq:gat)
     gene            1343..1430
                     /locus_tag="VVL45_000249"
     tRNA            1343..1430
                     /locus_tag="VVL45_000249"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1377..1379,aa:Ser,seq:gct)
     gene            complement(1556..2491)
                     /locus_tag="VVL45_000250"
     CDS             complement(1556..2491)
                     /locus_tag="VVL45_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEU4186492.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTAIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
     gene            complement(2488..3348)
                     /locus_tag="VVL45_000251"
     CDS             complement(2488..3348)
                     /locus_tag="VVL45_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU4186493.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFHYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKAPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            3456..5618
                     /locus_tag="VVL45_000252"
     CDS             3456..5618
                     /locus_tag="VVL45_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEU4186494.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHNVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATIASYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIVTLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIMLSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPGYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHEIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMMRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRMTPLETRGHVAMMGNLGYELDLTNLSDKEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTASNEAAVQFNYKNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     ELQENGEVYSGAELMYAGLTVILSQGDFLSKQYIFRRL"
     gene            5653..6903
                     /locus_tag="VVL45_000253"
     CDS             5653..6903
                     /locus_tag="VVL45_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019313951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU4186495.1"
                     /translation="MKWYKKAGFLLVAGASLLGLAACGQNNQSTDGKITIEFFNQKTE
                     MADTLQKIVDNFEKDHPTIDVKLTTVPAAGIVLKTRILSGDVPDIVNIYPQNMDFQEW
                     AKAGYFADMTGKSYLENIKNDYAEKYAINNKIYSVPLTANLYGIYYNKTKFKELGLEE
                     PKTFKEFQEIVKKIKDRGNSPFAVAGNEGWTLNGYHQLSLITITGSGDAANNYLRFSK
                     PNAISVDDTILKADAERLDLLADNAQDGWRGASYNDAVVAFSNEKALMMPQGSWALAA
                     INQQDPKFDVGMFAFPGEEVGKDVTVGAGDMALSTSATTKHPKETEEFISYMTSPKAM
                     QAYYDVDGSPVAVKGVQEKEDSALAEISKLAFTDKHYVWLGQHWNSEEDFFNLSAGYL
                     MDKNLKNMANNLNAFFNPMKADLD"
     gene            6922..7794
                     /locus_tag="VVL45_000254"
     CDS             6922..7794
                     /locus_tag="VVL45_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU4186496.1"
                     /translation="MAIRKFLNKYWGWTFLLIPLALQAIFFYFPMVQGAFYSLTNWTG
                     LTYNYKFVGLNNYKLLMIDGKFFTAIAFTLILTLALIIGEITIGMVVARALNSKMKGQ
                     TFFRAWFFFPAVLSGLTVSLIFKQFFNYGLPTIGKILGISFLQESLLGTPIGAVVATI
                     FVLLWQGVAMPIILFLAGLQSIPSDILEAASIDGATSKQTFWKIELPYLLPTISMVFI
                     LALKSGLTAFDQIFALTSGGPNNATTSLGLLVYNYAFKSNQYGYANAIALILFLIIGI
                     VSFIQIKLSKKFEI"
     gene            7811..8644
                     /locus_tag="VVL45_000255"
     CDS             7811..8644
                     /locus_tag="VVL45_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU4186497.1"
                     /translation="MKKEEKLNQFWKYVLLIVGGILILVPLLVTVFSSFKTTKDIMNH
                     FFGLPNPFTLSNYERLVSDGIGGYFWNLAVITVLSLIVVAFFIPAAAYSIARNMSKKK
                     AFAIMYSLLILGIFVPFQVIMIPITVMMSKLGLTNMWGLVLLYLTYAIPQTLFLYVGY
                     IKISVPDSLDEAAEIDGADRFTTYRRIIFPMLKPMHATTLIINALWFWNDFMLPLLIL
                     NKDSKMWTLPLFQYNYQGQYFNDYGPSFASYIVGIVTITVVYLIFQKHIISGMSNGAV
                     K"
     gene            8893..10335
                     /gene="gtfA"
                     /locus_tag="VVL45_000256"
     CDS             8893..10335
                     /gene="gtfA"
                     /locus_tag="VVL45_000256"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEU4186498.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGERYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKDLFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRSVVKALLNLFSFRNRSEAFDLEGTIEIETPTVHSIVIKRQ
                     NKDKSVTAVAEIDLQNQTYRVIENGVEVTF"
     gene            10473..11666
                     /locus_tag="VVL45_000257"
     CDS             10473..11666
                     /locus_tag="VVL45_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU4186499.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            12353..14332
                     /locus_tag="VVL45_000258"
     CDS             12353..14332
                     /locus_tag="VVL45_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU4186500.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAARETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KVVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            14399..15895
                     /locus_tag="VVL45_000259"
     CDS             14399..15895
                     /locus_tag="VVL45_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4186501.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGALTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPGTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPAAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            15895..16821
                     /locus_tag="VVL45_000260"
     CDS             15895..16821
                     /locus_tag="VVL45_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4186502.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            16830..17897
                     /locus_tag="VVL45_000261"
     CDS             16830..17897
                     /locus_tag="VVL45_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4186503.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            17908..18834
                     /locus_tag="VVL45_000262"
     CDS             17908..18834
                     /locus_tag="VVL45_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4186504.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
     gene            complement(18909..20235)
                     /locus_tag="VVL45_000263"
                     /pseudo
     CDS             complement(18909..20235)
                     /locus_tag="VVL45_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(20390..21100)
                     /gene="treR"
                     /locus_tag="VVL45_000264"
     CDS             complement(20390..21100)
                     /gene="treR"
                     /locus_tag="VVL45_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEU4186505.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            21285..23252
                     /gene="treP"
                     /locus_tag="VVL45_000265"
     CDS             21285..23252
                     /gene="treP"
                     /locus_tag="VVL45_000265"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEU4186506.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QRVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKGLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATDPIFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
     gene            23430..25055
                     /gene="treC"
                     /locus_tag="VVL45_000266"
     CDS             23430..25055
                     /gene="treC"
                     /locus_tag="VVL45_000266"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEU4186507.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCVGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNSNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKDINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            25311..25559
                     /locus_tag="VVL45_000267"
     CDS             25311..25559
                     /locus_tag="VVL45_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEU4186508.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            25766..26560
                     /gene="racE"
                     /locus_tag="VVL45_000268"
     CDS             25766..26560
                     /gene="racE"
                     /locus_tag="VVL45_000268"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEU4186509.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            26557..27528
                     /locus_tag="VVL45_000269"
     CDS             26557..27528
                     /locus_tag="VVL45_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEU4186510.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            27504..28025
                     /locus_tag="VVL45_000270"
     CDS             27504..28025
                     /locus_tag="VVL45_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU4186511.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWLEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            28022..28483
                     /locus_tag="VVL45_000271"
     CDS             28022..28483
                     /locus_tag="VVL45_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU4186512.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            28474..29214
                     /gene="xerD"
                     /locus_tag="VVL45_000272"
     CDS             28474..29214
                     /gene="xerD"
                     /locus_tag="VVL45_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEU4186513.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVT
                     IPTALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            29214..29942
                     /locus_tag="VVL45_000273"
     CDS             29214..29942
                     /locus_tag="VVL45_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEU4186514.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLTFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            29961..30530
                     /locus_tag="VVL45_000274"
     CDS             29961..30530
                     /locus_tag="VVL45_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEU4186515.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            30517..31239
                     /locus_tag="VVL45_000275"
     CDS             30517..31239
                     /locus_tag="VVL45_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEU4186516.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            31239..31481
                     /gene="yidD"
                     /locus_tag="VVL45_000276"
     CDS             31239..31481
                     /gene="yidD"
                     /locus_tag="VVL45_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEU4186517.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDRFSLKRNQEGE"
     gene            complement(31706..32671)
                     /locus_tag="VVL45_000277"
     CDS             complement(31706..32671)
                     /locus_tag="VVL45_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU4186518.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELTKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(32767..33519)
                     /locus_tag="VVL45_000278"
     CDS             complement(32767..33519)
                     /locus_tag="VVL45_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4186519.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            complement(33516..34472)
                     /locus_tag="VVL45_000279"
     CDS             complement(33516..34472)
                     /locus_tag="VVL45_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEU4186520.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLALQGYLKTLMKQDLVFVLLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFILANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRSRQ"
     gene            complement(34462..35421)
                     /locus_tag="VVL45_000280"
     CDS             complement(34462..35421)
                     /locus_tag="VVL45_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4186521.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTHKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            complement(35844..36545)
                     /gene="galE"
                     /locus_tag="VVL45_000281"
     CDS             complement(35844..36545)
                     /gene="galE"
                     /locus_tag="VVL45_000281"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU4186522.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYQVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            36946..38010
                     /gene="pepA"
                     /locus_tag="VVL45_000282"
     CDS             36946..38010
                     /gene="pepA"
                     /locus_tag="VVL45_000282"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEU4186523.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            38032..38328
                     /locus_tag="VVL45_000283"
     CDS             38032..38328
                     /locus_tag="VVL45_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEU4186524.1"
                     /translation="MVEPNLESLIKDLYNHARHDLSEDLVAALLETTKKLPTTNEQLQ
                     AVRLSGLVNRELLLNPKHPAPELLNLARFVKREEAKYRGTATSALMYEELFKML"
     gene            39010..39453
                     /locus_tag="VVL45_000284"
     CDS             39010..39453
                     /locus_tag="VVL45_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU4186525.1"
                     /translation="MKDGHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKSQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKGNEV"
     gene            39457..40221
                     /locus_tag="VVL45_000285"
     CDS             39457..40221
                     /locus_tag="VVL45_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU4186526.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFREDENG
                     CIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHDIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            40236..40964
                     /locus_tag="VVL45_000286"
     CDS             40236..40964
                     /locus_tag="VVL45_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4186527.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKVPATDFVADLFGGSVHD"
     gene            40957..42477
                     /locus_tag="VVL45_000287"
     CDS             40957..42477
                     /locus_tag="VVL45_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEU4186528.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPNLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLAALIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLVQKEKENLVIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGKTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVSKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMEPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            42822..43628
                     /locus_tag="VVL45_000288"
     CDS             42822..43628
                     /locus_tag="VVL45_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEU4186529.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(43795..44331)
                     /locus_tag="VVL45_000289"
     CDS             complement(43795..44331)
                     /locus_tag="VVL45_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU4186530.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKDYETTTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFQKNQDHIASLLFS
                     KNDYFLRQLQRELEHHVYPMLAADLKQTHPSLPTSYLQHFVVTNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            44464..45354
                     /locus_tag="VVL45_000290"
     CDS             44464..45354
                     /locus_tag="VVL45_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEU4186531.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDV
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGAVVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSDLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            45598..45951
                     /locus_tag="VVL45_000291"
     CDS             45598..45951
                     /locus_tag="VVL45_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU4186532.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            45952..46989
                     /locus_tag="VVL45_000292"
     CDS             45952..46989
                     /locus_tag="VVL45_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU4186533.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(47035..48033)
                     /gene="galR"
                     /locus_tag="VVL45_000293"
     CDS             complement(47035..48033)
                     /gene="galR"
                     /locus_tag="VVL45_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HEU4186534.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIQDKRLENFKNYSQARGIYHDELVFQGR
                     FTAQSGYDLMKEAIQSLGDQLPPAFFAASDSLAIGALQEAGISLPDRVSLISFNDTSL
                     TKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQE"
     gene            48198..49376
                     /locus_tag="VVL45_000294"
     CDS             48198..49376
                     /locus_tag="VVL45_000294"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEU4186535.1"
                     /translation="MAQHLTTEALRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVSLDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQVALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            49395..50876
                     /locus_tag="VVL45_000295"
     CDS             49395..50876
                     /locus_tag="VVL45_000295"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEU4186536.1"
                     /translation="MTLVNKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLETIEDSQTAREILGAELMDLVTPCPSQVNRDFWATYAHSPE
                     QAIEDFYQLSQKNDYIKLKAIARNIAYRVPSDYGELEITINLSKPEKDPKEIVAAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVDQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKAFRFAGFEQVKAGIVKWPMSVLRLTSDSKEDLINLADKILQEWRQYSDPAVQ
                     ILAETDRTPHHTITPIARKRDGQFELDLVLRDNQTSAEYPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFARVLEDAGVYKQTEQGQTAFMRFVEQVGILLD"
     gene            50946..51350
                     /locus_tag="VVL45_000296"
     CDS             50946..51350
                     /locus_tag="VVL45_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU4186537.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERRVRI"
     gene            51531..52295
                     /locus_tag="VVL45_000297"
     CDS             51531..52295
                     /locus_tag="VVL45_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-2 restriction enzyme DpnI"
                     /protein_id="HEU4186538.1"
                     /translation="MELHFNLELVETYKSNSQKARILTEDWVYRQSYCPNCGNNPLNH
                     FENNRPVADFYCNHCSEEFELKSKKGNFSSTINDGAYATMMKRVQADNNPNFFFLTYT
                     KNFEVNNFLVLPKQFVTPKSIIQRKPLAPTARRAGWIGCNIDLSQVPSKGRIFLVQDG
                     QVRDPEKVTKEFKQGLFLRKSSLSSRGWTIEILNCIDKIEGSEFTLEDMYRFESDLKN
                     IFVKNNHIKEKIRQQLQILRDKEIIEFKGRGKYRKL"
     gene            52292..52753
                     /locus_tag="VVL45_000298"
     CDS             52292..52753
                     /locus_tag="VVL45_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpnD"
                     /protein_id="HEU4186539.1"
                     /translation="MKTKQLVASEEVYDFLKVIWPDYETESRYDNLSLIVCTLSDPDC
                     VRWLSENMKFGDEKQLALMKEKYGWEVGDKLPEWLHSSYHRLLLIGELLESNLKLKKY
                     TVEITETLSRLVSIEAENPDEAERLVREKYKSCEIVLDADDFQDYDTSIYE"
     gene            complement(52803..54065)
                     /locus_tag="VVL45_000299"
     CDS             complement(52803..54065)
                     /locus_tag="VVL45_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEU4186540.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LAAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(54065..54529)
                     /locus_tag="VVL45_000300"
     CDS             complement(54065..54529)
                     /locus_tag="VVL45_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEU4186541.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFATTGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAKGLIQIIE
                     QAGATVQAIGIVIEKSFQDGRDLLEKAGYPVLSLARLDCFENGQVVFKEADL"
     regulatory      complement(54920..55015)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(55398..55526)
                     /locus_tag="VVL45_000301"
     CDS             complement(55398..55526)
                     /locus_tag="VVL45_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEU4186542.1"
                     /translation="MKANYPIYRPLTKRKFRVSDPYIYPHKFAVLDPDGYFLRFSE"
     gene            complement(55637..55780)
                     /locus_tag="VVL45_000302"
     CDS             complement(55637..55780)
                     /locus_tag="VVL45_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEU4186543.1"
                     /translation="MDYNAVIPELLVSNIEQSRSFYCGLLGFRIEYQRPEENFLFLLK
                     SVN"
     gene            complement(55792..56619)
                     /gene="xth"
                     /locus_tag="VVL45_000303"
     CDS             complement(55792..56619)
                     /gene="xth"
                     /locus_tag="VVL45_000303"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEU4186544.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHLEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDAKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            56752..57208
                     /locus_tag="VVL45_000304"
                     /pseudo
     CDS             56752..57208
                     /locus_tag="VVL45_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            57205..57342
                     /locus_tag="VVL45_000305"
     CDS             57205..57342
                     /locus_tag="VVL45_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4186545.1"
                     /translation="MTLNYIEILIKLVLTLKWLNNNVHFVKRLIDGKPTLLIKNGNID
                     P"
     gene            57361..57735
                     /locus_tag="VVL45_000306"
     CDS             57361..57735
                     /locus_tag="VVL45_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEU4186546.1"
                     /translation="MSASDVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENPKY
                     PVVTDGVIQVDVLESIGRSEEWLLDNLSKQGHDNVANIFIAEYDKGAVTVVTYEKEKP
                     GVLYSSKISSNRVNVALPYVGY"
     gene            57863..59608
                     /locus_tag="VVL45_000307"
     CDS             57863..59608
                     /locus_tag="VVL45_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4186547.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLVVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADFILVLQNGQIIERGTHEDLLAMDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            59598..61349
                     /locus_tag="VVL45_000308"
     CDS             59598..61349
                     /locus_tag="VVL45_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4186548.1"
                     /translation="MQNKQEQWAVLKRLMSYLKPYGLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLVYANRSVALDALFLRPAMSLLKLLGYAVLMAYFGYRGFSIG
                     ITAGTMYAFIQYINRLFDPLIEVTQNFSTLQTSMVSAGRVFALIDERTYEPLQENGQA
                     KVKEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLTLGG
                     TYHKMYSLQAGAMAYTL"
     gene            61522..62214
                     /locus_tag="VVL45_000309"
     CDS             61522..62214
                     /locus_tag="VVL45_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU4186549.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWFLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFEVLK"
     gene            62257..63483
                     /locus_tag="VVL45_000310"
     CDS             62257..63483
                     /locus_tag="VVL45_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEU4186550.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFKNEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
BASE COUNT        18364 a        11899 c        14407 g        19203 t
ORIGIN      
        1 aggtcccgta gtgtagcggt tatcacgtcg ccctgtcacg gcgaagatcg cgggttcgat
       61 tcccgtcggg accgtttaag gtaacgcaag ttattttaga ctcgttagct cagttggtag
      121 agcaattgac ttttaatcaa tgggtcactg gttcgagccc agtacgggtc atatatgcgg
      181 gtttggcgga attggcagac gcaccagatt taggatctgg cgcttaacgg cgtgggggtt
      241 caagtccctt aacccgcatt aagatataac aaatgagccg gcttagctca gttggtagag
      301 catctgattt gtaatcagag ggtcgcgtgt tcaagtcatg tagccggcat ttttagatag
      361 aagaaaacag tgcgaacgta gttcagtggt agaacaccac cttgccaagg tgggggtcgc
      421 gggttcgaat cccgtcgttc gcttagagag gccggggtgg cggaactggc agacgcacag
      481 gacttaaaat cctgcgattg gtaacgatcg taccggttcg attccggtcc tcggcataat
      541 atggagcacc cttagctcaa ctggatagag tacctgacta cgaatcaggc ggttagaggt
      601 tcgactcctc tagggtgcat ttttctattt aactcgggaa gtagctcagc ttggtagagt
      661 acttggtttg ggaccaaggt gtcgcaggtt cgaatcctgt cttcccgatt catggcggtg
      721 tagctcagct ggctagagcg tccggttcat acccgggagg tcgggggttc gatccccttc
      781 gccgctataa tgatcttgtc ggacctttag ctcagctggt tagagctctc ggctcataac
      841 cgagtggtcg taggttcaag tcctacaagg tccatttgaa tattggagga ttacccaagt
      901 ccggctgaag ggaacggtct tgaaaaccgt caggcgtgta aaagcgtgcg tgggttcgaa
      961 tcccacatcc tccttttata ttattaacgc gggatggagc agctcggtag ctcgtcgggc
     1021 tcataacccg aaggtcgtag gttcaaatcc tgctcccgca ataaggctcg gtagctcagt
     1081 tggtagagca atggattgaa gctccatgtg tcggcggttc gattccgtct cgcgccattt
     1141 tattgatagt atttatgcgg gtgtagttta gtggtaaaac tacagccttc caagctgttg
     1201 tcgcgagttc gattctcgtc acccgctttg aactttgttc aaattaccaa gttttttaac
     1261 ttgggcgcgt agctcaggtg gttagagcgc acgcctgata agcgtgaggt cggtggttcg
     1321 agtccactcg tgcccattaa ttggagaatt actcaagagg ctgaagagga cggtttgcta
     1381 aatcgttagg tcgggtaact ggcgcaaggg ttcgaatccc ttattctccg ttttaatgag
     1441 agtttgtaac tctctttttt atttttagag acaataaagc ttcagatccg taaaacttaa
     1501 ttgaattact aatctgaagt tatttttata tcgtttatat tataattgtg ttattttact
     1561 tccaactatt gagagaaatt tcgccactat ttagccagat ttcttttccg ttatcacatt
     1621 gaactaaaag ttttccattt tctgagatgt ctttagcaag tcccttgtag tctttttgct
     1681 ctagtgtgaa agtgacttct tttcctagaa tgaatgactg ttttttgtat aggtataata
     1741 gctcttctgc tggtgtttcg aagaaagcac gccagatttc tatgatcaat tcattccttg
     1801 ttataggagc tgtagcttta aataagctgg cagctttttc ttttaattcc tgagggaagt
     1861 ctttaatagt gaaattgata cctactccaa taatgatatc tgtgactaag ccagtttcta
     1921 cagaggtcat tgcttcagta aggattcctc caattttatg attgtttaga tagatatcat
     1981 tgacccattt tatgtcgaca tctattaaag ttaggttctt aatggctttg tagacagctc
     2041 cagctacaag tagtgtgtag gatggtaatt tgtcataggg gagatttggt ttaagatgga
     2101 gtgtcatata aataccacct tgtggtgagt agaaggaacg ttgaaaacgg cctcggcctg
     2161 ctgtttgata ggaagctaga tagagggtat ttgcttcatg gcctaaatca attgcttctt
     2221 ttgcatctag ttgtgttgat tttgtttcgg gtttaaagct gactttaatt ggaagatttt
     2281 cttctagaat ctctggaaga ataaggtcac cattcatcag tttatatcct ctatttttga
     2341 tactatcaat ttcaatgcct tcttgttcta gtcgcttgat ggctttccaa attgctgttc
     2401 ggcttaggga tagtttttct gcgatttttt ctccgctgat atagtcggtt tctttagata
     2461 ggatttggta gacagcttgg taggatttca taatgttgcc tttcttacta gttggtattg
     2521 agagtattct tttcttgtat gacttggagt ctgattaaag tattgtttat aagctttcga
     2581 aaaatggagt ggatctgaaa aacctaccga gtatgcaatt accttgatgg actcttgggt
     2641 attttcgaga agttgtctag ctcggtgcat tcgaacgtag agtaggtatt ctttgggtga
     2701 taaggtatta aattctttga atacgcttga taagtagctt ctgtgaacgg atagttcttt
     2761 tgctaaatct tgaattgtaa gtgattgagg atagtggcta tcaattaatc gtttgcattc
     2821 aagatagagt tggtgggttg atgaaatatt cttttttttc tgattgggag caatagttcc
     2881 cagatgaaac atcagttcat gaagttgtcc catgatatgg agttgagcta attcacttga
     2941 ttttgtaatc tgagcgaagc ggacaatgtc tgagatgagt tttgcagtag tctgggtatg
     3001 acaagtttca gattggatga gataggattg atcagaaatt tgggaaagag caaaataatc
     3061 aggggctttc cctccagtga ttcctaacca gtagtaggcc caaggttctt tactatctgc
     3121 ttgataaaag gttagttcct ctggttttaa tagaaagaaa tctccttctt ttaaatcaac
     3181 aattttaccc ttgtaatgaa attttccttg tcctttagta atgtaatgta ggacgtatgt
     3241 atcacgaatg gctggaccaa aagagtaatt aggtgtgcat tcctcatatc cataaaagct
     3301 tagggcaagg tcaattgttc cagtctggta ttctgaaaaa actagcatgt gctacctcct
     3361 acctaacatt ttaccatatt cttgagacat ttttctattt tggaaagcgg tttcaggtga
     3421 taaaatataa tcaataaagt gatgaggtga agtaaatggg agttaggata gagaataatc
     3481 tattttatgt tgagagtaaa aatctaagtt tgattattga aaatcgaaat ggctacttac
     3541 ttttgaaaca tttaggaaag actattaaga actataaagg ttccaatagt gtttatgaac
     3601 gagaccatgc cttttcagga aatccaacgg ctactaatcg aacctttagt ttagatactc
     3661 agcgacagat ttttggacaa catggcttag gagattttag gaaaccaacc atacaggttc
     3721 agcataatgt aactgaagta acagactttc gatttgtaga agcaaagatt ttaaaaggtc
     3781 agaatggtcc acagggctta ccttctccac atagcatgga cgatacagag actcttgtct
     3841 taatgttaga agattctaag gctcaactta gtctgacttt gtattatact acttttaata
     3901 atgatgcgac tattgctagc tacagtaaat tagataataa tagtaatcag gaagttgtca
     3961 tccataaaga tttttctttt atggctgatt ttccagctgc agattacgaa atagtaactc
     4021 tgcagggtgc ttatgctcgt gaaaagactg ttagacgtca acaggtagaa caaggaatct
     4081 tttcgattag ctcaaaccga ggtgcttctg gtcatgctca aacaccagct cttctactat
     4141 gcgaacaagg agtcacagag gatgctggga atgtgtttgc tattcaacta atgtatagtg
     4201 gcaactttga agcttttgtt caaaaaaatc aattgaatga agttcgggtg gctattggca
     4261 ttaatccaga aaacttttct tggaagttag ctcctgagga atactttgaa acaccggtag
     4321 ctttagtgac ccattcagat cagggattaa ctggtattag tcatgaaagt cagaattttg
     4381 tactgaagca cattatgcta agtgaatttt ctaaaaaaga acgtccaatt ctaatcaata
     4441 actgggaagc tacttacttt gactttcaga gagaaaaact gttagagtta gcagatgaag
     4501 ctaagaaagt tggcattgaa ctttttgtat tagatgatgg ttggtttggc aatcgttttg
     4561 atgataatcg tgctttaggt gattgggttg ttaatgagga aaaactgggt ggaagtctag
     4621 aaagtctgat ttcagctatc catgaaagag gtttgcagtt tggactttgg ttagaacccg
     4681 aaatgatttc tgtagatagt gatttgtatc gtcaacaccc tgactgggct attcaggttc
     4741 ctggctatga gcatacttat tctcggaatc aattagtact taatcttgcc aatcctcagg
     4801 tagtagaata cttgaaaagt gtcttagatc aactcctatc ttatcatgag attgattaca
     4861 ttaaatggga tatgaaccgc aatatcacta agctagggaa tggattaact tatctagaga
     4921 cacagatgca atctcatcag tacatgctgg ggctttacga actcgtttct tatctgacag
     4981 agaagcacag ccatattctc tttgagtcct gctctggtgg tggtggacga aatgatcttg
     5041 gtatgatgcg ctatttccca caagtctggg ctagtgataa tactgatgcc attgcacgtt
     5101 taccaattca atacggttca tcctatctct atccaaccat ttctatgggg gctcatgtgt
     5161 cagcagtacc gaatcatcag atgggacgaa tgacaccatt agaaacacgt ggccatgtag
     5221 caatgatggg aaatttgggc tatgagcttg atttgacaaa tttatcagat aaagagaaag
     5281 ctacgattgc taatcaggtg aacttgtata aagaattacg accagtagtt cagttaggac
     5341 agcagtatag actaattaat cctgatactg cctccaatga agcagctgta caatttaatt
     5401 acaaaaatca aacgattgta acctacgttc gcgttttatc agttgtagag accatggaaa
     5461 caactttaaa gttaaaagat ttggatgaag agggactgta tgaattacag gaaaatggcg
     5521 aagtttactc aggtgcagaa ctcatgtatg caggtttaac tgttatctta tcccagggag
     5581 attttttgag taaacagtat atttttagaa gactataata aaggtgtata aatttataaa
     5641 ggaggctttc caatgaaatg gtataagaaa gcaggttttc ttttagtagc tggggctagt
     5701 ttactaggcc tagcagcttg tggtcagaat aatcaatcga ctgatggaaa gataacgatt
     5761 gaatttttta accagaagac cgaaatggcg gatactttgc aaaaaattgt agataatttt
     5821 gaaaaggatc atcctactat tgatgtaaaa ctgacgactg ttccggcagc tggaattgtt
     5881 ctgaagactc ggattttatc aggagatgtt ccagatattg ttaatatcta tcctcaaaat
     5941 atggattttc aggagtgggc aaaagcaggt tactttgcag atatgacagg gaagtcctat
     6001 cttgagaata ttaagaatga ctatgccgaa aagtatgcaa tcaataacaa gatttatagt
     6061 gtacctttaa ctgctaacct ttatggaatc tattacaata aaacaaagtt taaagaatta
     6121 ggacttgagg aaccgaagac ttttaaagag tttcaagaga ttgttaaaaa gataaaagat
     6181 agagggaatt ctccgtttgc agttgcaggc aatgaaggat ggacattaaa cggttatcac
     6241 caactttctc tcattactat tacaggcagt ggagacgcag ctaataacta tcttcgcttt
     6301 tcaaaaccaa atgctatttc tgtagatgat actattttaa aagcagatgc agaacgacta
     6361 gatttgttgg cagacaatgc tcaagatgga tggcgtggtg cctcttataa tgatgctgtg
     6421 gtagcattct cgaatgaaaa agccttgatg atgccacaag gatcatgggc attagcggca
     6481 attaatcaac aggatccaaa atttgatgtg gggatgtttg ccttcccagg agaagaagta
     6541 ggaaaagatg ttactgttgg tgcaggggac atggcattat caacttcagc tactaccaaa
     6601 catcctaaag aaaccgagga atttatcagc tatatgacta gtccaaaagc tatgcaagca
     6661 tattatgatg tggatggatc accagttgct gtaaaaggtg tacaggaaaa agaagattca
     6721 gcgcttgcag agatttctaa actagcattt acggataaac attatgtttg gttgggccaa
     6781 cactggaatt ctgaagaaga ttttttcaat ctaagtgcag gttacttgat ggataaaaat
     6841 ctgaaaaata tggcaaacaa tctcaatgct ttctttaatc caatgaaggc agatttggac
     6901 tagaatagga gttaagatga tatggctatt cgaaaatttt taaataaata ctggggttgg
     6961 acttttttgc tcatcccgct tgcattacaa gctatcttct tttactttcc gatggtacaa
     7021 ggtgctttct atagtttgac taactggact ggattgacct ataattataa atttgttggt
     7081 ctgaataact acaaattgct tatgattgat gggaaattct ttacagccat tgcttttact
     7141 ttgattttaa cactggcatt gattattgga gagattacaa ttgggatggt tgtggctcgg
     7201 gccttgaatt ctaagatgaa agggcagacc ttctttagag catggttctt tttcccagcg
     7261 gttttgtctg gtttgacagt ttccttgatt tttaaacaat tctttaacta tggtcttcca
     7321 acgattggaa aaattttagg gattagtttt ttacaagaaa gtctattagg aacaccgatc
     7381 ggtgcggtag tagcaaccat ttttgttctt ctatggcaag gagtagcaat gccaattatt
     7441 ctctttcttg ctggtcttca gagtattcca agtgatattt tggaggcggc atcaattgat
     7501 ggtgcaacaa gcaaacaaac tttttggaag attgaattac catatttgct accaacgatt
     7561 tctatggttt ttatcctagc tcttaagtct ggtttgactg cctttgacca aatttttgcc
     7621 ttgacgagtg gtggcccgaa taatgctaca acgtctcttg gtcttttagt ctataactat
     7681 gccttcaaga gtaatcaata tggatatgcg aatgcaattg ccttgatttt attcttaatt
     7741 attggaattg tttcttttat ccaaatcaaa ctatcaaaga aatttgaaat ctaatagggg
     7801 agtcttacac atgaaaaaag aagaaaaatt gaatcagttt tggaagtatg tcctcttgat
     7861 agtaggtggt attctcatac ttgttccttt gttggtaacg gtttttagtt ccttcaagac
     7921 aaccaaggat atcatgaatc atttctttgg tttgcccaat ccttttacat taagcaatta
     7981 tgaacgattg gtttcggatg ggattggagg ctatttctgg aatttagctg ttattacggt
     8041 gttatcattg attgtagtag ccttctttat accagctgca gcctattcca ttgctcgaaa
     8101 tatgtccaag aaaaaagcct ttgcaattat gtattcgctc ttgatcttag ggatatttgt
     8161 tccatttcag gtgatcatga ttccgataac agttatgatg agcaaactcg gcctgacaaa
     8221 tatgtgggga ttggtactac tttatctaac ttatgcaatt ccacagaccc tcttcttgta
     8281 tgttggttat attaaaatca gtgtaccaga tagtttagat gaagctgcag aaattgatgg
     8341 ggctgatcga tttacaactt accgtcgaat tatctttcca atgctgaagc ccatgcatgc
     8401 tacaaccttg attatcaatg cattatggtt ctggaatgac tttatgttgc cattgttgat
     8461 tttgaataag gattccaaga tgtggacttt gcctctcttc cagtacaact accaaggtca
     8521 gtactttaat gactatgggc caagttttgc atcctatatt gtaggaattg taacaattac
     8581 tgtcgtctac ctcatcttcc aaaaacatat tatttcagga atgagcaacg gagcagtgaa
     8641 ataagataca aagggacgat tcagcttgct gaaaatttct gtagaaaaat aggaaattga
     8701 cgtagtgtgc ttgcacacaa gaagatttat cttttttcct aagaaattag tcccgtgttc
     8761 aattccgtat acaaagggac gattcagctt gctgatttca atcgtaccct atgaagcaat
     8821 tcttaaaata cataaactca taaaagccaa gtcatatgag acttggcttc aattaaaaaa
     8881 aggagagtaa tgatgccaat tcagaataaa accatgttga ttacctattc tgatagcctt
     8941 ggaaataatc ttaaagactt atatgataat ttggaagagc attttggaga tgctattgga
     9001 ggagttcacc ttttaccatt tttcccatca acaggtgatc gtggatttgc gccagttgac
     9061 tacgacgaag tggactcagc ttttggtgat tgggaggatg ttaagcgttt aggtgagaga
     9121 tattatctta tgtttgattt tatgattaat catatttctc gtcaatccaa gtattataag
     9181 gactatcaag aaaaacatga agccagtgaa tttaaagatc tctttttaaa ctgggataag
     9241 ttttggccag aaaaccgtcc gacacagtct gatgtagatt taatttacaa gcgtaaggat
     9301 cgtgcaccaa agcaagagat tgtgtttgaa gatggttcag tggaacattt gtggaatacc
     9361 tttggtgagg agcagattga tcttgatgtg accaaagaag taactatgga atttatccgt
     9421 aagaccattc agcacttggc aagtaatggg tgtgatttga ttcgtctaga tgcctttgct
     9481 tatgcagtga agaaattgga tactaatgat ttctttgtgg aaccagatat ttgggattta
     9541 ttggacaaag ttcgagatat cgctgctgag tatgggacag agcttttacc tgagattcat
     9601 gaacactatt cgattcagtt taaaatagca gaccatgatt actatgttta tgattttgct
     9661 cttccaatgg tgacacttta tactctttac agttccagaa cagagcgttt ggctaagtgg
     9721 ttaaagatga gcccgatgaa gcaatttacg acgctagata cccatgatgg gattggagta
     9781 gtagatgtca aggatatcct gaccgatgag gagattgact atgcttcaaa tgaactctat
     9841 aaggttggag ccaatgtcaa acgtaagtac tctagtgccg agtataacaa cttagatatc
     9901 taccaaatca attcaaccta ctattcagcg cttggagatg atgatgtcaa gtattttctc
     9961 gctcgtctaa ttcaagcttt tgccccaggt attcctcagg tttactatgt gggtctatta
    10021 gcaggcaaga atgacttgaa attattagaa gaaactaaag aaggtcgaaa tattaatcgt
    10081 cattactata gcaacgagga aatagcaaaa gaagtgcaac gatctgttgt gaaggccctt
    10141 ctcaatctat tttctttccg taaccgttca gaagcctttg atctagaagg gactattgag
    10201 atagagacac caacagttca cagcattgta atcaaacgtc aaaataaaga taagtccgta
    10261 acagcagtag cagaaattga tttgcaaaat cagacttatc gggtaattga gaatggagtt
    10321 gaagtaacat tttgaagcct tgatatggtg gataaaatag ggtttttatt ttggaaaacg
    10381 tttcctttgt tttcaaattg ctaaaaaagt ggtacaatat aggatagctt actattatct
    10441 gaatcagctg atttggagag aaaggattca ttttgaaatc aataggcttt attgaaaagc
    10501 tgaaggggtt gtctagtaaa gagctgattt tattgggaat tatcctaagt atctttttac
    10561 ccttttatct ttttgtagtt gtactctgtt tatatattat cagtttgatt tttacaggag
    10621 acatgaaaag tattcttcag aaaatggggg agcatccgat gctgcttctt tttcttagct
    10681 atagtactgt tatatccatt cttgcacaaa attggatggg tcttgtggct tcagtaggaa
    10741 tgtttctatt tactattttc tttttgcact atcagtcgat tttatcccat aaattctttc
    10801 gattgatttt gcagttcgtc ttgtttggta gtgtcttgtc agctgctttt gccagtttag
    10861 aacatttcca aattgtgaag aaatttaact atgcttttct ttcacccaat atgcaggtgt
    10921 ggcatcagaa ccgggcagaa gtgactttct ttaatcctaa ttattatgga attatttgtt
    10981 gtttctgtat tatgattgct ttctatctgt ttacaacgac caagttgaat tggttgaaag
    11041 tattctgtgt gattgcaggc tttgttaatc tctttggttt gaactttact caaaatcgaa
    11101 ctgcctttcc tgctattatc gctggagcaa ttatctatct ctttacgact attaaaaact
    11161 ggaaggcctt ttggcttagt attggggtct tcgcgattgg tttgagtttc ctcttttcta
    11221 gtgatttggg agttcgaatg ggtactttag actcttctat ggaagaacgc atttctatct
    11281 gggatgctgg gatggccttg tttaagcaaa atcctttttg gggtgaaggg ccattgacct
    11341 atatgcactc ttatcctcgg atacatgctc cttatcatga acatgcccac agtctttata
    11401 ttgatacgat tctgagttac ggaattgtgg gtaccatttt attagttttg tcttctgttg
    11461 ctcctgttcg cttgatgatg gatatgagtc aggagtcggg gaaacgtccg attatcggcc
    11521 tttatctatc tttccttaca gtggttgctg tgcacggaat ttttgacttg gctctcttct
    11581 ggattcagtc aggctttatt ttcttgctag ttatgtgcag tattccattg gagcatcgaa
    11641 tgttggtatc ggacatgacg gattaagttt tataagtttg aaatcttcta ccttgggtag
    11701 gagatttttt tatacgatgg gcatgtcgtg catctgaggt gtaagtcctc tgtagggatc
    11761 cactaccggt gaatacaata gcgctcccca agcctgacta tcgtgaggta gcggattaaa
    11821 atggtctggg gatagatcgt tttaagtctg acgcaggaaa taagaattgt cagagaaagc
    11881 aatagtgaaa tcgtaggcta tgaatagaaa cgtgatagcg aggagcatat agtggataag
    11941 gtagcgagaa attttaaagt ataaaagata gtcttaatct atatatgtaa atcacaaaag
    12001 taattgaatt cggaataagg ttggtgtgaa aagacatagt aaactgaatc ttgaatacta
    12061 gaatgatact tctacaatat tgttataatt taatttgaat tccaaaatca cattgttccg
    12121 tccgtatttc attttactgt atgtcttcct aaactccaaa gtttctgcgt caaatttcct
    12181 attttcactg tagtctttga cacttcgaac gacaatttga atcatatatt caaaaaaaaa
    12241 tgaaaaattt gtgccaacaa gcctaaatat tctgaaaatt tgtttgattt ttaatggata
    12301 atgtgatata atggttgtca atctataatt cacataaaca gaggagtaga aaatgaaaaa
    12361 aaatcgtgta tttgctacag caggtcttgt tttattagca gcaggtgtac ttgcagcatg
    12421 cagttcttca aaatcatctg attcatcagc ccctaaagct tatggctatg tttatacagc
    12481 agacccagaa accttggact acctgatttc aagtaaaaat agtacaacag tagtgacttc
    12541 aaatgggatt gatggtttat tcactaacga taattacggt aatcttgctc ctgcagttgc
    12601 agaggattgg gaagtctcta aggatggttt gacctacact tataagattc gtaaaggggt
    12661 taaatggttt acctctgatg gagaagaata tgcagaggtg acggctaaag atttcgtgaa
    12721 cggtttaaaa cacgcagcag ataaaaaatc agaagctatg tatttagctg aaaattcggt
    12781 taaaggcttg gcagattatc tatcaggaac ttcaacagat ttttcaacag ttggtgtcaa
    12841 ggcggttgat gattatacgt tacaatacac tttgaaccag cctgaaccgt tctggaactc
    12901 taagttgacc tattctattt tctggcctct gaatgaagaa ttcgaaacat caaaaggaag
    12961 cgattttgct aaaccaacag atccgacatc cttgctttat aatggtccat tcttgttgaa
    13021 agggttgact gcaaaatctt ctgtagagtt tgtaaaaaat gagcaatatt gggataaaga
    13081 aaatgtccac ctagatacta tcaatctagc ttactatgat ggatcagatc aggagtcgct
    13141 agagcgtaac ttcactagtg gagcttatag ttatgcccgt ctttacccta ccagctccaa
    13201 ctattctaag gttgcagaag aatacaagga caatatctat tacacacaat caggctctgg
    13261 gattgctggt ctgggtgtga atattgatcg ccaaagttac aactatactt ctaaaactac
    13321 agattcagag aaagtagcta ctaagaaggc attgcttaac aaagatttcc gtcaagcctt
    13381 gaattttgct cttgatcgct cagcttactc agctcaaatc aatggtaaag atggagcagc
    13441 tttagcagtt cgtaatttat ttgtaaaacc agactttgtt tcagctggtg agaagacctt
    13501 tggtgattta gtcgctgctc aacttcctgc ttatggtgat gagtggaaag gtgtgaattt
    13561 agctgatggg caggatggtt tattcaatgc tgacaaggcc aaggcagagt ttgcgaaagc
    13621 taagaaagct ttagaagcag acggcgttca gtttcctatt catctggacg ttccagtaga
    13681 ccaagcatca aaaaactaca tatctcgtat tcagtccttt aaacaatctg tagaaacagt
    13741 tcttggtgtt gaaaatgtcg ttgttgatat tcaacaaatg acaagtgatg aattccttaa
    13801 tattacttac tatgctgcca atgcttcatc tgaggattgg gatgtatcag gaggagtttc
    13861 atgggggcca gactatcaag acccatctac ttacctggat attttaaaaa caactagcag
    13921 tgaaactaca aaaacatatt taggatttga taatccaaat agcccttcag tagttcaagt
    13981 tggtttgaaa gaatacgata aattagttga tgaagctgcc agagagacaa gcgacttgaa
    14041 tgtccgttat gaaaaatatg cagcggctca agcatggttg acagatagtt cactctttat
    14101 tcctgctatg gcttcttctg gtgcagcacc agtgctttca cgaattgttc catttactgg
    14161 agcttctgcg caaacaggct ctaaggggtc agatgtttac ttcaaatatt tgaaattaca
    14221 agataaagtg gtgactaagg aagagtatga aaaagctcgt gaaaaatggt tgaaagaaaa
    14281 agctgaatca aatgagaaag ctcaaaaaga attggcaagt catgtgaagt aaataatttc
    14341 taatagaact ttctctccat gaggagaagg ttcttttggg atttttaaag gaaataatat
    14401 gaaaaaatat atttttatgc gtgttttgcg gtcattggtt tcgattttct tagtaacgac
    14461 tttgacctac acgattatct ataccttggt tcctcgaaaa ttgattttca agcaggatcc
    14521 taactataat aaaattgcga caacggctga taaacgtgat aactatgaaa atactgtgtt
    14581 tgagcgtatg ggctacattg agtattacga tactaaagag ttgcaagaaa aggcaagtag
    14641 catggattct tctgtaacag tagaagcaaa tgcgaccaat aaagctattt atgaaaagta
    14701 catcaatcaa ttaggtcatg gttggacttt gggagaattt actgaaagtg gtcaattcta
    14761 tgctactcgt gaaattccaa tttttgaacg tgtttttcac ttctatgcta acttgattga
    14821 cattgaccat acaaataaaa tccaagaccc tgaaaatcca gacttgaaac gctaccttcg
    14881 ttttgaaaat gatccagcta tcggatggtc attggtcggt tcaggaacta aacataaata
    14941 tctcttgtac tttaacagtc agttcccatt tgttcatcaa aactttgtga acttgaattt
    15001 aggtgactct tacccaacct atgctaatac accagttctt caggttatta ctcaaggtca
    15061 aggacaaacc aaaactgccc aagttcagtt cccaacaggt aagaaaacgt cttctgtaaa
    15121 tatttactca agaacctaca agtcacctag tcaggctgac tctcgtgaag tagctagcta
    15181 tgggaaagat gatccttata cagcgactga aagtaattac caatatccat ctatgattgt
    15241 cagctctgct attactggtt tgattggttt ggttcttgcc tatgctcttg ccgtgccact
    15301 tggttcagcc atggctcgtt tcaagaacac ttggattgat agcctctcaa caggggcttt
    15361 gaccttcttg cttgctcttc caacgattgc cttggtttac atcgttcgat tgattggatc
    15421 atctattgcc cttccagatt cattccctat cttgggagct ggagattggc gttcttacgt
    15481 tttaccagca gtcatccttg gtttgttggg tgctcctggt acagccattt ggattcgtcg
    15541 ttacatgatt gacttgcaat ctcaagactt tgttcgtttc gctcgtgcaa aaggtttgtc
    15601 tgaaaaagaa atttcaaaca aacacatctt taaaaatgcc atggttccgc tggtttcagg
    15661 aattcctgct gccattattg gggttatcgg tggtgcaacc cttactgaaa cagtcttcgc
    15721 cttcccaggt atgggtaaaa tgttgattga ctctgtaaaa gcatctaata actctatggt
    15781 cgttggtctt gtcttcatct ttacatgtat ttctatcttc tcacgtcttt tgggagatat
    15841 ttggatgact attattgacc cacgtattaa attgactgag aaaggaggca aataatgtct
    15901 acaatcgata aagaaaaatt tcagtttgta aaacgtgacg attttgcctc tgaaactatt
    15961 gatgcgccag catattctta ctggaaatca gtgtttaaac aatttatgaa gaaaaaatca
    16021 actgtagtca tgttgggaat cttggtagcc atcattttga taagtttcat ctacccaatg
    16081 ttttctaagt ttgatttcaa tgatgtcagc aaggtaaacg actttagtgt tcgttatatc
    16141 aagccaaatg cggagcattg gttcggtact gacagtaacg gtaaatcgct ctttgacggt
    16201 gtctggttcg gagctcgtaa ctccatcctc atttctgtga ttgcgacagt gattaacttg
    16261 gttatcggtg tttttgtcgg tggtatttgg ggtatttcaa aatcagttga ccgtgtcatg
    16321 atggaagttt acaacgtcat ctcaaacatc ccacctcttt tgattgttat tgtcttgact
    16381 tactcaatcg gagctggatt ctggaatctg atttttgcca tgagcgtaac aacatggatt
    16441 ggtattgcct tcatgatccg tgtgcaaatc ttgcgctatc gtgacttgga atacaacttg
    16501 gcgtcacgta ctttgggaac accaaccttg aagattgttg ccaaaaatat catgcctcaa
    16561 ttggtatctg ttattgtgac aaccatgact caaatgcttc caagctttat ctcatacgaa
    16621 gccttcttgt ctttcttcgg tcttggatta ccgattacag tgccaagttt gggtcgtttg
    16681 atttcggatt attcacaaaa cgtaacaacc aatgcttact tgttctggat tccattgaca
    16741 acccttgtct tggtatcctt gtcccttttc gtagttggtc aaaacttagc ggatgctagt
    16801 gatccacgta cacatagata ggagtagaaa tgacaaaaga aaaaaatgta attttgactg
    16861 ctcgcgatat tgtcgtggaa tttgacgttc gtgacaaagt attgacagcc attcgcggcg
    16921 tttcccttga actagtcgaa ggagaagtat tagccttggt aggtgagtca ggatcaggta
    16981 aatctgtttt gacaaagacc ttcacaggta tgctcgaaga aaatggtcgt attgcccaag
    17041 gtagtattga ctaccgtggt caggacttga cagctttatc ttctcacaag gattgggaac
    17101 aaattcgtgg tgctaagatt gcgactatct tccaggaccc aatgactagt ttggacccca
    17161 ttaaaacaat tggtagtcag attacagaag ttattgtaaa acaccaagga aaaacagcta
    17221 aagaagcgaa agaattggcc attgactaca tgaataaggt tggcattcca gacgcagata
    17281 gacgttttaa tgaataccca ttccaatatt ctggaggaat gcgtcaacgt atcgttattg
    17341 ctattgccct tgcctgccga cctgatgtct tgatctgtga tgagccaaca actgccttgg
    17401 atgtaactat tcaagctcag attattgatt tgctaaaatc tttacaaaac gagtatcatt
    17461 tcacaacaat ctttattacc cacgaccttg gtgtggtggc aagtattgcg gataaggtag
    17521 cggttatgta tgcaggagaa atcgttgagt atggaacggt tgaggaagtc ttctatgacc
    17581 ctcgccatcc atatacatgg agtctcttgt ctagcttgcc tcagcttgct gatgataaag
    17641 gggatcttta ctcaatccca ggaacacctc cgtcacttta tactgacctg aaaggggatg
    17701 cttttgcctt gcgttctgac tacgcaatgc agattgactt cgaacaaaaa gctcctcaat
    17761 tctcagtatc agagacacat tgggctaaaa cttggcttct tcatgaggat gctccgaaag
    17821 tagaaaaacc agctgtgatt gcaaatctcc atgataagat ccgtgaaaaa atgggatttg
    17881 cccatctggc tgactaggag gaaggaaatg tctgaaaaat tagtagaaat caaagattta
    17941 gaaatttcct tcggtgaagg aagtaagaag tttgtcgcgg ttaaaaatgc taacttcttt
    18001 atcaacaagg gagaaacttt ctcgcttgta ggtgagtccg gtagtgggaa aacaactatt
    18061 ggtcgtgcta tcatcggtct aaatgataca agtaatggag atatcatttt tgatggtcaa
    18121 aagattaatg gtaagaaatc gcgtgaacaa gctgcggaat tgattcgtcg aatccagatg
    18181 attttccaag accctgccgc aagtttgaat gaacgtgcga ctgttgatta tattatttct
    18241 gaaggtcttt acaatcaccg tttatttaag gatgaagaag aacgtaaaga gaaagttcaa
    18301 aatattatcc gtgaagtagg tcttcttgct gagcacttga ctcgttaccc tcatgaattc
    18361 tcaggcggtc aacgtcaacg tatcggtatt gcccgtgcct tggtcatgca accagacttt
    18421 gttattgcag atgagccaat ttcagccttg gacgtttctg tacgtgccca agtcttgaac
    18481 ttgctcaaaa aattccaaaa agagctcggc ctgacctatc tcttcatcgc ccatgacttg
    18541 tcggttgttc gctttatttc agatcgtatc gcagttattt acaagggtgt tattgtagag
    18601 gttgcagaaa cagaagaatt gtttaacaat ccaattcacc catatactca agccttgctt
    18661 tcagcggtac caatcccaga tccaatcttg gaacgtaaga aggtcttgaa ggtttacgat
    18721 ccaagtcaac acgactatga gactgataag ccatctatgg tagaaatccg tccaggtcac
    18781 tatgtttggg cgaaccaagc cgaattggca cgttatcaaa aaggactaaa ctagtaatgg
    18841 ttttataatt tccatgtcaa tttttatgga aattaggctc tttgtcaact gtagtgggtt
    18901 gaagtcagct aagctcgaga aaggacaaat ttcgtccttt cttttttgat gttcaaagcg
    18961 ataaaaatcc gttttttgaa gttttcaaag tttcgaaaac caaaggcatt gcgcttgata
    19021 agtttgatga gattattggt cgcttccagt ttggcattag aatagtgtag ttgaagggcg
    19081 ttgataatct tttctttatc tttgagaaag gttttaaaga cagtctgaaa aagaggatga
    19141 acctgcttca gattgtcctc aatgagtccg aaaaatttct cagggtcttt gttctgaaag
    19201 tgaaaaagta agagttgata gatctgatag tggtgtttca agtcttctga atagcttaaa
    19261 atcttgtcaa gaatttcttt atttgttaag tgcatgcgaa aagtagggcg ataaaatcgc
    19321 ttatcactca gtttacggct atcctgttga atgagtttcc agtagcgctt gatagccttg
    19381 tattcatggg attttcgatg aaactgattc atgatttgga catgcacacg actcatagca
    19441 cggctaagat gttgtacaat gtgaaagcga tcaagaacga ttttagcatt cgggagtgaa
    19501 acagcctggg agactgtttc agcctgagct tagaaatttg aaagcgaagc tgtttagcca
    19561 agtcatagta agggctaaac atagccatag taatgatttt gacgcgacat cggacggctc
    19621 tatcatattt aagaaagtga tctcggatga cagcttgtgt tctaccttca agaacagtga
    19681 tgatattgaa attgttaaaa tcttgcgcaa taaagctcat ctccatctcc cgattgaaac
    19741 agtcactccc cggactgttt caacgtccca ggacataatc tcaggaagac gcgaaaaatc
    19801 atgctcaaag tgagaatcat tgagctttcg aatgacagtt gaagttgaaa tggccagctg
    19861 atgagcaata tcggtcatag aaatcttctc aatcaacttt tgcgcaattt tttggttgat
    19921 aatacgagga atttgatgat tcttcttgac gatagaagtt tcagcgacca tcattttaga
    19981 acaatgatag cacttgaaac gacgctttct aaggagaatt ctagtaggca taccagttgt
    20041 ttcgaggtaa gggatcttag acggtttttg aaagtcatat ttcttcatta gacttccaca
    20101 atcagggcaa gatggagcct cataatccag cttagcgata atttctttgt gggtatccat
    20161 attgatgatg tctagaatct tgatgtttgg gtctttaata tcgagcagtt ttgtgataaa
    20221 atgtaattgt tccatatgaa tctttctaat gagttgtttt gtcgcttttc attataggtc
    20281 atatgggact ttttttctac acaaaatagg ctccataata tccatagggg ggttacccac
    20341 tacaaatatt atagagccga aaaaggctga gatctggtgt ctcagtcttt tatttcttgc
    20401 gttttgcaaa atctacaaat ctaaatttct ctaacttatg gcgactttcg gtaaactgaa
    20461 attggcgtcc gtcttggaga tagacttttg atttaatcga aacgacataa gggtctttgc
    20521 caatgtccat gagaatcttg tctcggtcgc ttgagtggtc aatggtgatt tccttctgag
    20581 cataatcaat aaggagtttg aggccatttt ctatataaga atagatagac tgctcagcaa
    20641 tttggcgagt gagatttggg atgagttcca tatccagata gtccgtatcc agaacggata
    20701 ccagatcatc caccacacgc tggcggacca ccttccaaac catccgaaac tctgggaaac
    20761 cggttatcag tgaggatttt ttatcaataa taatcttgtc cagactgacc acgttggttt
    20821 tagagcgcag tccaagttct ttaactagtt cttggtagct ggttaggttg gatacaggga
    20881 aattgacggt ttcttctttg acgacttgag aaccttgccc tcttatcttt ttgatcaatc
    20941 cttcctcttg gaggagagac agggcctttc ggacggtatc acgactcact tgatattgct
    21001 ccataaggtc gtgctcgcta ggaaggaaat ctccgacagc gtaagtctcg ttttgaatgg
    21061 tttcttggat ttgcttaaat aattgttggt atttcttcat tttgcctgct taaataggtt
    21121 tttagtctag tttaatgaac acctgtatct attttaccat acaagtgcta gaaaatttac
    21181 aaaaaaacac cctttttata ccaaacaagt tgtagacaag tttggtatcg tttacaatat
    21241 acttatcaaa tcaaacttgc tttgacaagt ataaggagaa tcaaatggga aaatttgaac
    21301 aagaagccaa agatctgctt caggcaattg gaggcaaaga aaatgtgact gccgtaactc
    21361 actgtgcgac acggatgcgc tttgttttag gagatgataa gaaggctaat gttaaagcta
    21421 tcgagtcaat tccagctgtt aaaggaactt ttacaaatgc aggtcaattt caggtaatca
    21481 ttggaaatga cgtgcccatc ttttataatg attttacagc cgtttcaggt attgagggtg
    21541 tttccaaaga agcagccaag tctgcagcta agagtaatca aaacgtggtc caacgtgtta
    21601 tgaccactct ggcggagatt tttactccga ttattccagc cttgatagtc ggaggattga
    21661 tcctcggttt ccgtaatgtc ttggaaggtg tccattggtc gatgttggat ggcaagacca
    21721 tcacagaatc ctctcagttt tgggcaggtg tcaatcactt cctctggttg cctggtgaag
    21781 ctatcttcca gttcttacca gtagggatta cttggtctgt ttctcgtaag atgggaacca
    21841 gccaaatttt gggaattgtt ctcggaatct gtttggtatc gcctcagttg ctcaatgcct
    21901 atgcggttgc ttcaacgcca gcagctgata tcgcggcaaa ctgggtttgg aattttggct
    21961 attttactgt taatcgtatc ggttaccaag cccaagttat cccagccttg cttgcaggtt
    22021 tgagtctgtc ttatcttgaa atcttctggc gcaagcatat cccagaagtc atttctatga
    22081 tttttgtacc tttcttgtca ttgattccag ccttgatttt ggctcatact gttttgggac
    22141 caatcggttg gacaattgga caaggacttt catcagttgt cttggcaggt ttaactggtc
    22201 cagttaaagg gctcttcggt gcaatttttg gtgccctcta cgctccattt gtcatcacag
    22261 gtctgcacca tatgaccaat gccattgata cacaattgat tgcggatgct ggtggcactg
    22321 ccctatggcc aatgattgct ctttctaata ttgctcaagg ctcagccgtg tttgcctatt
    22381 atttcatgca tcgccatgat gagcgtgagg ctcaggtttc acttcctgca accatttcag
    22441 cctatctcgg tgttacagaa ccagctcttt ttggggttaa cgtaaaatat atttatccat
    22501 ttgttgctgg gatgactggt tcagcccttg caggcatgtt atccgttact tttaatgtaa
    22561 ctgcggcttc tattggtatc ggtggtttgc caggtattct ctctattcaa cctcaataca
    22621 tgctgccatt tgcaggaact atgctagttg cgattgttgt tccaatgctc ttgactttct
    22681 tcttccgcaa ggctggtctc tttacaaaaa cagagggcga tacgaacttg caggcagaat
    22741 tcgttgctca agaagaagca gaatttgtga accatgaacc agtagaactt acttcggtag
    22801 aaattatcag cccactaact ggccaagtga aagaattgag tcaagcgacg gatcctattt
    22861 ttgcatcagg tgtcatgggg caaggtctgg tcattgaacc aagccaaggt gagttgacct
    22921 ctccagttaa tgggacagtg acggttcttt tccctaccaa gcatgccatc ggcattgtct
    22981 ctgacgaggg agttgaattg ctcatccaca tcggtatgga tacagtaggt cttgatggca
    23041 aaggttttga aagtcttgta gtccaaggag atcacgttac agttggtcag caactgattc
    23101 gttttgatat ggatgtcatt aaggctgcag gtctggtgac agaaactcct gttatcatca
    23161 ccaaccaaga tgcttataca gcgactattc ccggaactta tccgacaacg attcaagctg
    23221 gagcatctct catggtcgct acacgaatct aatactcttc gaaaatctct tcaaaccacg
    23281 tcagctctat ctgcaacctc aaaacagtgt tttgagcaac ctgtggctag cttcctagtt
    23341 tgctctttga ttttcattga gtataattga aatagcacaa atagggacgg aagactgctt
    23401 cctccctttt atcagaaaag gagaaacaga tgacacttga taaaggaaaa gtagtctatc
    23461 aaatctatcc aaaatcttac aaagacacca ctgaaaacgg tattggggat ttccgtggga
    23521 ttatcgaaaa aattccctat ttagccaaac taggcgtgga tatggtctgg ctcaatccat
    23581 tctatccaag ccctcaacgg gataatggtt acgatatttc agattatatg gcagtggatc
    23641 ctctttttgg tgatatggct gattttgagg aaatggtgtg tgtcggtaaa gagcacaaga
    23701 ttgactttat gctggacatg gtacttaatc attgttcgac agagcatgaa tggtttcaga
    23761 aagccctagc tggtgataag tattatcaag actttttctt catccaagac caaccaacag
    23821 attggcagtc taagtttggt ggttctgcat gggcgccttt cggggatact gggaaatact
    23881 atcttcacct atttgatgag acccaggctg accttaactg gcgcaattcc aatgtccgta
    23941 aggagctttt caaggttgtt aatttctggc gcgacaaggg tgtcaaaggt ttccgatttg
    24001 atgtgatcaa tttgatcggt aaggacgagg tttcagtgga ctgtcctgaa aatgaaggga
    24061 agccagctta tacagacaaa cccatcgttc ataactattt gcgtatgatg aaccaagcca
    24121 cttttggatc tgacgatagc tttatgacag ttggggaaat gtcttctacc accatggaaa
    24181 actgtgtcct ctattcgtcg cctgatcgtc aggaattatc catgaccttt aattttcatc
    24241 acctcaaggt ggactataag gatggacaaa agtggacctt ggctcccttt gattttgaag
    24301 agttgaagag tctttaccat agttggggta aggaaatgag tgataaagac ggttggagtg
    24361 ccctcttttg gaacaatcac gatcaaccac gtgccttgaa ccgttttgtc gatattcaaa
    24421 acttccgcaa ggaaggagcc accatgctag cggccagcat tcacctgtca cgtgggacac
    24481 cttatatcta catgggtgag gaaatcggga tgattgaccc agactatgat tctatggctg
    24541 attatgtgga tgtcgaatcg cttaatgcct atcagatgct cttagaagaa ggaaagagtc
    24601 agcaagaagc cttccagatt attcaagcta agtcgcgtga taattcacgc attcccatgc
    24661 agtgggatgc ttcggaaaat gcaggatttt caacaggcac tccttggttg aaagctggta
    24721 aatcctataa agacatcaat gtagaaaatg aaatccaggg tccgattttt accttctatc
    24781 aagacttgat tcgactgcgt aaggaaatgc caatcatttc agaaggaagt tacaaaccag
    24841 cctttgaaga tagcaaacaa gtctacgctt ttgaacgcca gtttgaggat caaaagttac
    24901 tagtgctcaa taacttttat gctaaagaag tggaaatcga cttgccagca gtctaccaaa
    24961 atggacagat tttgatttca aactatgaag atgcagaagt gtctgagaaa attctactca
    25021 aaccctatca aacactggct atttatgtaa attaatactc ttcgaaaatc tcttcaaacc
    25081 gtgtcagttt tatctgcaac ctcaaagcgg tgctttgagc aacctgtggc tagcttccta
    25141 gtttgctctt tgattttcat tgagtataaa cttagcaagg agacctcttt ttcagaaaag
    25201 cagggttctt tttgctgtct attccatcct tgaaagtgta gaacaaactg gtcagggtgg
    25261 ctagtttgtg gtataatgaa agggactcaa aaagaaacag gagaaaaatg atggatttac
    25321 ttttagcaat tgtattgatt gtgctagctt ttctaggagg agctcttgga ggaatgtact
    25381 tggttcgtaa gcaaatcgaa aaagaatttg ctgacaaccc acgtttgaat gctgaagcag
    25441 ttcgtactct tttgagtgca aatggtcaaa aaccaagcga agctaaggta caacaagttt
    25501 accaccaaat catccgccaa caaaaggcag cccttgctaa caataaaaag aaaaaataaa
    25561 taaggaaaag tctgggatga aagttccaga cttctcacta tgttggttat ggtttaggga
    25621 tgagactttt tccttgcatt ctattgatat ttgattatga ttaagagaga ggtagttgac
    25681 attctatcaa gctgtcggtc agttcgttca gtacaaggaa tcataatgaa cgatcaatca
    25741 gaaaaaagac tagaaagaag actgtatgga taatcgacca attggttttt tggattcggg
    25801 tgtcgggggc ttgaccgttg tgcgcgagct catgcgccag cttccccatg aagaaatcgt
    25861 ctatattgga gattcggcgc gggcgcccta tggcccccgt cctgctgagc aaattcgtga
    25921 atatacttgg cagctggtca actttctctt gaccaaggat gtcaaaatga ttgtcattgc
    25981 ttgtaacact gcgactgcgg tcgtctggga agaaatcaag gctcaactag atattcctgt
    26041 cttgggtgta attttgccag gagcttcggc agccatcaag tccagtcaag gtgggaaaat
    26101 cggagtgatt ggaacgccca tgacggtaca atcagacata taccgtcaga aaatccatga
    26161 tctggatccc gacttacagg tggagagctt ggcctgtccc aagtttgctc ccttggttga
    26221 gtcaggtgcc ctgtcaacca gtgttaccaa gaaggtggtc tatgaaaccc tgcgtccctt
    26281 ggttggaaag gtggatagcc tgattttggg ctgtactcat tatccactcc ttcgccctat
    26341 tatccaaaat gtgatggggc caaaggttca gctcatcgat agtggggcag agtgcgtacg
    26401 ggatatctca gtcttactca attattttga aatcaatcgt ggtcgcgatg ctggaccact
    26461 ccatcaccgt ttttacacaa cagccagtag ccaaagtttt gcacaaattg gtgaagaatg
    26521 gctggaaaaa gagattcatg tggagcatgt agaattatga caaataaaat ttatgaatat
    26581 aaggatgacc aggactggta tgttgggtct tatagtattt ttggtggcgt taacagtttg
    26641 agcgactata agacagattt tcctctgttt gaattctcca aaatatttgg agatgaagag
    26701 tatggtttcc cgctttcagt tactgtttta cgctatggtt ctacctaccg tttgttctcc
    26761 tttgtggtag atatgcttaa tcaagaaatg ggacgaaact tggaagttat tcaacgtcat
    26821 ggggccctgc tcttggttga aaatgggcaa ctcttgtatg tagaattgcc taaagaaggg
    26881 gtcaatgttc atgatttctt tgagacaagc aaggtcagag aaaccttgtt gattgcgact
    26941 cgtaacgaag gcaaaaccaa ggaattccga gctatctttg ataagttagg ctacgatgtg
    27001 gaaaatctta atgactaccc tgacctgcct gaagtagcag aaacaggtat gacctttgaa
    27061 gaaaatgccc gccttaaggc agaaaccatt tctcaattaa cgggtaagat ggttttggca
    27121 gatgattctg gtctcaaagt cgatgtcctt ggtggcttac caggcgtctg gtcagctcgt
    27181 ttcgcaggtg tgggagcaac tgaccgtgaa aataatgcca aactcttgca cgaattggcc
    27241 atggtctttg aactcaagga ccgctcggct cagttccaca caaccctagt cgtagccagc
    27301 ccaaataagg aaagtttagt tgttgaagca gactggtcag gttatattaa ctttgaacct
    27361 aagggtgaaa atggctttgg ctatgatccc ctcttccttg taggagagac aggtgagtca
    27421 tcagctgaat taaccctaga agaaaaaaat agtcaatctc accgtgcctt agccgttaag
    27481 aaacttttgg aggtatttcc atcatggcaa agcaaaccat cattgtaatg agcgattccc
    27541 atggcgatag cttgattgtg gaagaagtcc gtgatcgcta tgtgggcaaa gtcgatgccg
    27601 tttttcataa cggcgattct gaactacgtc cggattctcc actttgggag ggcatccgcg
    27661 ttgttaaagg gaacatggac ttctacgccg gctacccaga acgtctggtg actgagcttg
    27721 gttcgaccaa gattatccaa actcatggtc acttgtttga catcaatttc aactttcaaa
    27781 agttggacta ctgggctcag gaggaagagg ccgctatctg cctctatggt cacttgcatg
    27841 tgccaagtgc ttggttggaa ggcaagatcc tctttctaaa tccaggttct atcagtcaac
    27901 cacgaggtac catcagagaa tgtctctatg ctcgtgtgga gattgatgat agttacttca
    27961 aagtggactt tttgacacga gatcacgagg tgtatccagg tttgtccaag gagtttagcc
    28021 gatgattgcc aaggagtttg agactttctt gttggggcag gaggaaactt ttttgacccc
    28081 tgctaaaaat ctagctgtgt tgattgatac ccacaatgcg gatcatgcga ccctcttgct
    28141 cagtcagatg acctataccc gtgttcccgt tgtgacagat gaaaaacagt ttgttgggac
    28201 gattggactc agagatatta tggcttatca gatggagcat gacttgagcc aagaaatcat
    28261 ggcggatacg gatatcgttc atatgacaaa aacggacgta gcggttgttt cgcctgattt
    28321 caccattacg gaggtcttgc acaagctagt agatgagtcc ttcttaccgg tcgtggatgc
    28381 agagggtatt ttccaaggga ttattacgcg caagtccatc ctcaaggccg ttaatgccct
    28441 cttgcatgac tttagtaagg aatatgagat tcgatgccaa tgagagacag gatttcagcc
    28501 tttttagagg aaaagcaggg cttgtctgtc aattccaagc agtcctataa gtatgatttg
    28561 gagcaatttt tagacatggt aggtgagcgg atttctgaga ccagtctcaa gatttaccaa
    28621 gcccagctag ccaatctaaa aatcagcgcc cagaagcgaa agatttcggc ctgtaaccaa
    28681 tttctatact ttctctatca aaaaggagag gtggacagct tttaccgctt ggaattagcc
    28741 aaacaagctg aaaagaagac ggaaaagcca gagattctat acctagactc tttttggcag
    28801 gaaagcgacc atccagaggg ccgcttgcta gcgctcttaa tcctagaaat ggggctcttg
    28861 cccagtgaga ttttagccat caaggttgcg gacatcaatc tggattttca ggtgctgcga
    28921 atcagcaagg cttcccaaca gaggattgtc accattccca cggccttgct ttcagaattg
    28981 gaacccttga tggggcagac ctatcttttt gaaagaggag agaaacccta ttctcgtcag
    29041 tgggcctttc gtcagttaga atcttttgtc aaggagaaag gttttccatc cttatcagct
    29101 caagtcttac gtgaacagtt tattctaaga caaatagaaa acaaggtcga tttgtacgaa
    29161 attgcaaaaa aattaggatt aaaaacagtc ctgaccttag aaaaatatag ataatggata
    29221 ttaaattaaa agattttgaa ggacccctgg acttgctctt gcatctggtt tctaagtacc
    29281 agatggatat ctacgatgtg cccattacgg aagtcatcga acagtatcta gcctatgtct
    29341 caaccctgca ggccatgcgt ctggaagtga cgggtgagta catggtcatg gctagtcagc
    29401 tcatgctgat taagagtcgt aaactccttc cgaaggtagc agaagtgaca gacttggggg
    29461 atgacctgga gcaggacctc ctctctcaaa tcgaagaata tcgcaagttc aagctcttgg
    29521 gtgagcactt ggaagccaag caccaagaac gggcccagta ttattccaaa gcgccgacag
    29581 agttgattta cgaagatgcg gagcttgtgc atgacaagac gaccattgac ctctttttga
    29641 ctttttcaaa tatcctagcc aagaaaaaag aggagtttgc acaaaatcac acgacgatct
    29701 tgcgggatga gtataagatt gaggacatga tgattatcgt gaaagagtcc ttgattggac
    29761 gagatcaatt gcgcttgcag gatttgttca aggaagccca gaatgtccaa gaggtcatca
    29821 ccctcttttt ggcaacccta gagttaatca aaacccagga gttgatcctc gtgcaagagg
    29881 agagttttgg agatatctat ctcatggaaa agaaggaaga aagtcaagtg cctcaaagct
    29941 agacttgata gagaggaaag atgagtactt tagcaaaaat agaagcgctc ttgtttgtag
    30001 cgggtgaaga tgggattcgg gtccgccagt tagctgaact cctctctctg ccaccgacag
    30061 gcatccagca aagtttagga aaattagccc agaagtatga aaaggaccca gattccagtt
    30121 tggctttgat tgagacaagt ggtgcttata gattggtgac caagcctcaa tttgcagaga
    30181 ttttgaagga atactctaag gcgcctatca accagagctt gtctcgggct gcccttgaga
    30241 ccttgtccat tattgcctac aaacagccga ttacgcggat agaaattgat gccatccgtg
    30301 gagttaactc gagtggagcc ttggcaaagt tgcaggcttt tgacctgata aaggaagacg
    30361 ggaaaaagga agtattgggg cgccccaacc tctatgtgac tacggattat ttcctagatt
    30421 acatggggat aaaccattta gaagaattac cagtgattga tgagcttgag attcaagccc
    30481 aagaaagcca attatttggt gaaaggatag aagaagatga gaatcaataa gtatattgcc
    30541 cacgcaggtg tggccagtag gagaaaagca gaagagctga ttaagcaagg cttggtgacg
    30601 gttaacggcc aagtggtgcg tgaactagca accactatca agtcaggcga caaggtcgaa
    30661 gttgaaggtc aacctatcta caacgaagaa aaggtctact atctgcttaa caaaccacgc
    30721 ggtgtgattt ccagtgtgac agatgataag ggtcgcaaga cggttgtcga cctcttgccc
    30781 aatgtcaaag agcgtattta ccctgtgggt cgtttggact gggatacatc aggtgtcttg
    30841 attttgacca atgatgggga ctttacagac gagatgattc accctcgtaa tgagattgac
    30901 aaggtttatg tcgcgcgtgt taaaggtgtg gccaataagg acaatctccg ccccttgacc
    30961 cgtggtcttg agattgatgg taagaaaacc aagccagctg tttatgaaat tctcaaagtg
    31021 gacccagtca aaaatcgctc tgtggtgcag ttgaccatcc atgaagggcg taaccatcag
    31081 gttaaaaaga tgtttgaagc tgttggtctc caagtagata agttgtctcg gactcgtttc
    31141 ggacacctag acttgacagg actccgtcca ggagaatccc gtcgtcttaa taaaaaagaa
    31201 atcagccaac tacacaccat ggctgtaact aagaaataat gaaacgaatt ttaatagcgc
    31261 ctgtgcgctt ttaccaacgt tttatctcac cagtctttcc accctcttgt cgctttgagc
    31321 tgacttgttc caactacatg attcaggcta ttgaaaaaca tgggtttaag ggggtattga
    31381 tgggcttggc tcggatttta cgttgtcatc cctggtcgaa aacaggtaag gaccccgttc
    31441 cagaccgctt ttcccttaaa cgaaatcaag aaggggaatg aggtggggta aatagatttc
    31501 aaaatgataa aaacgcatcc tatcaggttt gagtgaactt gataggatgc gttttagaat
    31561 gtcaaaattt tatactcttc gaaaatctct tcaaaccgcg tcagctttca tctgcaacct
    31621 caaaacagtg ttttgagcaa cctgcggcta gtttcctagt ttgctctttg attttcattg
    31681 agtattaaat tgagtttgaa gtggcttatt tcaaagcttt ttgtatgtct tcaatcatga
    31741 gttttgttga ttcaagtccg cctccgctta gataccagag gtctggtgtt agttggataa
    31801 tcttaccatt tttagcagca ggtgtttcag cgataagggc attttctagg acaccgtcgt
    31861 tgctagagtt gtccccaccg atggcaaggg tacggttgat gacaaagagg atgtcagggt
    31921 tgatttcttt gacactttca aagctgactt cttgtccgtg gcgtgagtct tcaaattttg
    31981 tatcagttgg tttgaatttc aaggtttggt acaagaaaga gaaacgagat ttggcaccaa
    32041 aggctgccat ttttccttca ttaaggagga tcgcaagggc ttttttgtca gagctttcat
    32101 ttttagtagc gacttcttgg atgctcttgt ctagcttggt caattcttcc ttggctttct
    32161 gtgtaccagt ttcgccgaag gcacttgcta aggattcgat attagccttg gtagaagtcc
    32221 agtagtcgtc cttgcttgct tggaagagaa cggttggggc gatttctttg aatttgtcta
    32281 cgaatttttg tgtacgtggc gaagcgataa tcaaatcagg ctcaagggcg gcgatagctt
    32341 ctaaatcagg ttctttcata gaaccaacat ttttgacagt tcccactagg tcttttagat
    32401 aagtcggaac agtttttgta ggcattccga cgatattttt ttcaaatcct aaagcgcgaa
    32461 tagtatccgc agcgccgagg tcaaaggtca caatcttttc aggaactttg gaaagtttga
    32521 cctcgtccag tgaactttta atggttacct ctgttggagc agagctactg gtctctgtct
    32581 gactagtgct tgagtttgta ctacatgcac caagtaggag caagaagctg gccactaggg
    32641 cagtgaaata aagtttaagg gatgttttca taatttctcc tttttaaaat gtgataacga
    32701 tttagggagt ctcttaatct tattgactaa gagactgaag gttctctaac ttgagctttt
    32761 atgttactag ctatagatac agatcttttt gtcattgata tcagctagcg tgatgggaat
    32821 ctcataaagt tgactgagca ggtcagcctg catgatttga tcggttcttc ccttgctaaa
    32881 gacctggccg tccttgaagg cgacaatttc atctgcatac tgactggcca tgttgatatc
    32941 gtggaggacg atgataatgg tcttgccgag ttcctccacc agtcgtcgaa gaatctgcat
    33001 catgctgacg ctttgcttga tatcgagatt gttgagtggt tcgtccagca agataaagtc
    33061 cgtatcctgg gccagtacca tagcgataaa gacgcgctgg agttgccccc ctgacaggct
    33121 attgatgtag cggtctttta agttggtcag ttctaaatag ttcagagttt ctcggatttt
    33181 ttcccagtct tctgatctaa gtcgacctcg gctgtaggga aaacgtccaa aactgaccag
    33241 ttcttcaaca gtcaatttgg cttggtaatt gattttctgt tttaggatgg ttagttcttg
    33301 ggccagttct tgcgaattcc agctctcgat ttcacgtcct ttgatactga gaactccctg
    33361 atctttcttg gttagcctgc tcatgatgga gaggagagtc gattttccag caccatttgg
    33421 accaataaag gctgtcagtt tttgaggact gacttcaagc gaaatgcctt gcaaaatatc
    33481 ctgtttttga atggatttgt caatgttttc cagtttcact gacgagacct cctatatagt
    33541 aagataaaga ataagaagcc acccacactc tcaatgatca tactgatacg aatttccagt
    33601 gcaaagactc gttcaatcaa ggcttgcccc aaggttaagc taataaatcc aaccagaatg
    33661 gccactataa agagtaactt gtgctgatag tctttgacaa tcaggtaggt gaggttggcc
    33721 agtataaagc cgaagaaggc cataggtcct accaaggcag tggccgttga ggtcaaaagc
    33781 acgattcccc agaggagctc tttctgttct ttttcaacat cgagtcccaa tatctgagcc
    33841 gtttctcttt gcaggtgcaa gacatctaga acgactgctt ttcgaaagaa aaagattgtc
    33901 aaagcgagga tgatcagaga accgatggct aggatggaag tgttgagatg ttgaaaggag
    33961 gcaaaaagac tattttgcag tttatcgtat tcgtttggat ccattaggac ttgaaggaag
    34021 gtgctgatat ttcgaaagag acttctgagc gctagacaga tcagcaggac gaagaccagg
    34081 tcttgcttca tcagtgtctt caagtaacct tgtaaggcga gaaagaagag ggactggaca
    34141 agaagtaaga ctaggaattc taagataggg gatttgccaa gttgaagaaa cttgctttca
    34201 aaaaccagta gtagggtttg tagtaggacg tagaaggatt caattcccaa aatactaggc
    34261 gtcaggaagc gattttccgt cagggtttga aaactaatgg tcgaaatccc agtcgcgatg
    34321 gctaccaaga gataaacgat gatcttttgg gaacgcaact tccaagcaaa ggctgacaag
    34381 tgagtgatgg gccaaaagta gagaagacaa gctccgatgg caagaataat gagaatccag
    34441 aagagcttgg tatgtttgct tttagtctgc atcttttcgt ccccctctcc agagaagtag
    34501 gataaagacg agactaccga tgattcctag caagagactg acagacaact catagggcct
    34561 aatcagaact cgggatagga tatcgcaagc cagaactaga ttggcaccaa ccagtgcgac
    34621 catgagtttg gtttgactta gattatctcc atagcgcttg cgaacaagat tgggaacgat
    34681 aactccgaga aatggtaggc cacccacggt aatcatggtg acgcttgtcg ttagcgccac
    34741 cagaaagagg gccagttttt caagtaggga gtaggaaatc cccaaactct cgctggtttc
    34801 tttccctaga ttcatgatgg tgaaggtttg ggataatttc caaacggtta tcaggatgat
    34861 gaggcctaag aagagccact catactgatg ggtctgaatc atggagaagg agccctgggt
    34921 ccaggcagtc atactctgaa ccagattgaa acgataggcg ataacttctg tgactgagcc
    34981 gataatcccg ctatagatga tcccaatcag aggcaacatc cacctttcct ttacagtaaa
    35041 aatggtcata aaggctagga agaagagggt gaatacgatg gatgaaacaa aagcgaagag
    35101 catcttgtgg gtcagactag ccgatggaaa gacaaaaagg ctcagcacca ttcccagttt
    35161 ggcggcttca gtcgttccaa ctgtactcgg tgcagcaaac tgattttggg taatagtctg
    35221 catgagaagg cctgccatac tcatactaga ggcagtcagg agaatactga tagttcttgg
    35281 gagacgggac tcttgaaaga ggagccaggt ctgctggtcg aaatcaaata gctttcccca
    35341 tgaaaaatca ctggtcccaa tgctaataga gagaaagact aggagtagaa gtaagccaat
    35401 taaataatga gaaagtttca taccccgtcc tttcatgtag atttggtatc gaaagatatc
    35461 tgcggatata aatgtaacat tatttttcta atctgtcaat aaaatttctg acaatttaat
    35521 aaatacaaca aggagagagc aacaagactt tctcctttgt tatcctattc taaaatgttt
    35581 ttaccttaat ctgataaaat aatatcttcg agggagtagc tagccgtcca atcaagatat
    35641 tgtttagctt ttgaagcatc tgctaggaca ctggctgggt cactagcacg tcgagcaaca
    35701 atctcgtgtg ggatttttta atttagtaat tcttcagcag ttttaaagat ttctttgata
    35761 gtatagcctt ttttagttcc taagttaaag atttgagaag aactgtcttc ttgaaatagg
    35821 tagttcattc ctttaacatg agcctatgca aggtccaaga cataaatgta atctcgaata
    35881 catgaaccgt cacgtgtatc gtagtcatct ccaaatattt ttaagctatc attttgtccc
    35941 aatgcggtct tgttgatatt tggaatgatg tgagttggat ttttcacacg cagaccgttt
    36001 gaagcatcca tttcagcccc agcaacatta aagtaacgga aaataacata tttccagtcg
    36061 tagcgattgg ccatccagta aatcattcgt tcgcccatca gttttgtctc tgcataaggg
    36121 ttgacagggt cgagcagggt atcttcagtc accggcttgt caatacagtt atttccatag
    36181 agagaagcag tcgaagagaa catgattttt tgaatgccaa cttcagataa gactttgaga
    36241 acttggttca taccagcaac gttggcagtg aagtatttac ttggattttc aatactttcg
    36301 ctcacaacaa tttcacctgc acaatggagg acagcatcaa tctgattttc ttctaagtaa
    36361 gcctttaagg cgctagcatc ataaacatcc agttctttaa agctagcacg actatccaca
    36421 gccgctcgat ttcctgtgga gagattatcg aggacatgca cctgatagcc agcatttaaa
    36481 agagctttaa cggtatggga gccaatgtag ctagtcccac ctgtaacaag aattgttttg
    36541 gtcatgattt tttccttttc tagttttgta ctatagtaaa ttgaaataag atatgaacaa
    36601 ctctattagg aaagtcaaat taatttctag aaatgtttta gcagctacag cgtactattc
    36661 caaactcaac caactatata tttaaagaaa gaagaaaggg aagtcaacta ttttctgtaa
    36721 atttcatgaa aaatccaaac aactttgatg gtgtattgtt tttttgtttg gaaataaata
    36781 ttcgagttaa ttttggaatg tttactagat tttgcactac atgcaagaga atactatcct
    36841 tttctaagat agagcaggac taagatacta acttagaaat ccaccttccc actatccttc
    36901 tcctataaaa agtggtaaaa tggatgaaag aaagaaggaa ctgaaatgac aacattattt
    36961 tcaaaaatta aagaagtaac agaacttgct gcagtctcag gtcatgaagc gcctgtccgt
    37021 gcttatcttc gtgaaaagtt gacaccgcat gtggatgaag tggtgacaga tggcttgggt
    37081 ggtatttttg gtatcaaaca ttcagaagct gtggatgcac cgcgcgtctt ggtcgcttct
    37141 catatggacg aagttggttt tatggtcagc gaaatcaagc cagatggtac cttccgtgtc
    37201 gtagaaatcg gtggctggaa ccccatggtg gttagcagcc aacgtttcaa actcttgact
    37261 cgtgatggtc atgaaattcc tgtgatttca ggttctgttc ctccgcattt gactcgtgga
    37321 aaggggggac caaccatgcc agccattgcc gatatcgttt ttgatggtgg ttttgcggac
    37381 aaggctgagg cagaaagttt tggcatccgt cctggtgata ccattgtacc agatagttct
    37441 gcaattttga cagccaatga aaaaaatatc atctcaaaag cttgggataa ccgctacggt
    37501 gtcctcatgg taagcgagct agctgaagct ttatcgggtc aaaaactcgg caatgaactc
    37561 tatctgggtt ctaacgtcca agaagaagtt ggtctgcgtg gcgctcatac ctctacaacc
    37621 aagtttgacc cagaagtctt cctcgcagtt gattgctcac cagcaggtga tgtctacggt
    37681 ggtcaaggca agattggaga tggaaccttg attcgtttct atgatccagg tcacttgctt
    37741 ctcccaggga tgaaggattt ccttttgaca acggctgaag aagctggtat caagtaccaa
    37801 tactactgtg gtaaaggcgg aacagatgca ggtgcagctc atctgaaaaa tggtggtgtc
    37861 ccatcaacaa ctatcggtgt ctgcgctcgt tatatccatt ctcaccaaac cctctatgca
    37921 atggatgact tcctagaagc gcaagctttc ttacaagcct tggtgaagaa attggatcgt
    37981 tcaacggttg atttgattaa acattattaa accataaggg aggtggtagg gatggtagaa
    38041 ccaaacctag aaagccttat aaaagatctt tacaatcatg ctcgacatga tttgagtgaa
    38101 gatttagttg ctgctctcct agagactact aaaaaactgc ctactacaaa tgagcaattg
    38161 caggcagttc gtctctcagg cctggtcaat cgtgaattgc tcctaaatcc caaacatcca
    38221 gcacctgagt tgctcaactt ggctcgcttt gtcaaaagag aagaagccaa gtacagagga
    38281 actgcgactt ctgcgcttat gtatgaggaa ctctttaaaa tgctttgatt ccattgtgga
    38341 tcaaagcatt ttttgtaaga tctatctcgt gagcgttcaa atagtagtaa caaggaatca
    38401 tgagaagtca gccgagccca tagtagtgaa gaaacttctg taatggaagt ggagcgaagt
    38461 gaggaatcgt caaacggtag gggataggct gtttgaaggg tgctacgaaa aagatagggt
    38521 aaggtgaaat tatagcggaa tgaaataaga tatgaacaaa ttgattgagg aaattaaatg
    38581 aatttataga aatcttttag cagtcatggt gtcctattcc agtttcgaaa tgctataaat
    38641 acgaacaaat tgattgggaa attcaaatcg ctttctatca atgttttaga agatacagtg
    38701 ctctagtcta acttcaatat actataaaag aacagatgga agcaattatg atttacttct
    38761 atctttaaaa taaatggtct gataggaaaa atgtgtcccc tcataaaata taggatagta
    38821 cgaatggaag gcttttgtag actggaggac gaaggagttt agaaactctc gttaaaagtt
    38881 agttgagccg gagtatggtg gtgtggataa ttatcaaaaa ttatttctaa atcgaaatag
    38941 ttgacagtta gaacgatgag tgttacaatt gttttattca tttcgatatc gaaataaatt
    39001 ggaggtgtta tgaaagatgg tcatttgcta gcccatcata ttcgtttgtt gaatgggcgg
    39061 atttttcaaa agttactgag tcaagatcct gaggctcttt ataggggtga acagggcaag
    39121 attttagcgg ttttatggaa tagtgaaact ggctgcgcaa ctgcgacaga tatcgcgctt
    39181 gcgactggac ttgcgaataa tacgctgacg actatgataa aaaagctaga ggaacaaaag
    39241 cttgtaattg ttagtccgtg tggaaaagac aagcgtaaga agtatttagt tttaacggag
    39301 ttaggcaagt cccagaaaga agtggggcat cgtgtcagtc agaaattgga tactatcttt
    39361 tacaaaggat tttcagagga agaaattcac caatttgaag gttttcaaga aagaattttg
    39421 gcgaatctga aagagaaggg aaatgaggtt tagaatatgt cacaacaagt gaaaaatgct
    39481 cacaacctat acatccatgc cattcaagac ggacgagttg ctgaggctca agcccagtct
    39541 gtaggggata cctatatcca acattcgaca ggtgttcctg acgggaaaga aggatttgca
    39601 gccttctttg cagatttctt tgagcgtcat ccagagcgtc agattaagat tgtccgcacc
    39661 attgaggatg gcaatctggt ctttgttcat gttcatcaat atctgaatgg tggagaagct
    39721 caatgggtga cgacggatac tttccgtgag gatgagaatg gttgtattgt tgagcattgg
    39781 gatgtcattg actactatcg aacacctgaa aatgaccaac tagatcaaat ttttggagat
    39841 tttgaaatca aggatttgga taagaaagca gaaaataaaa agctggttcg ccgtttcttg
    39901 acagaaattt tccaaaatgg ggagctagag cagtggagtg attatgtggc agacgatttg
    39961 attcagcata atcatgatat tggacaagga agtgctgctt ataaaaacta tgtggctgaa
    40021 tatagtgtca cttttgactt tgttttccaa ctcttaggac aaggaaacta tgtggttagc
    40081 tatggtcaga ctcagattga tggcgttgct tatgcccagt acgatatctt ccgtttagag
    40141 aacgggaaaa ttgtggagca ttgggataat aaggaagtta tgcctaaggt agaagacttg
    40201 accaatcgag ggaagtttta aattgaggac aaagaatgat tgaatacaaa aatgtagcac
    40261 tgcgctacac agaaaaggat gtcttgagag atgtcaactt acagattgag gatggggaat
    40321 ttatggtttt agtagggcct tctgggtcag gtaagacgac catgctcaag atgattaacc
    40381 gtcttttgga accaactgat ggaaatattt atatggatgg gaagcgcatc aaagactatg
    40441 atgagcgtga acttcgtctt tctactggtt atgttttaca ggctattgct ctttttccaa
    40501 atctaacagt tgcggaaaat attgctctca ttcctgaaat gaaggggtgg agcaaggaag
    40561 aaattacgaa gaaaacagaa gagcttttgg ctaaggttgg tttaccagta gccgagtatg
    40621 ggcatcgctt acctagtgaa ttatctggtg gagaacagca acgggtcggt attgtccgag
    40681 ctatgattgg tcagcccaag attttcctca tggatgaacc cttttcggcc ttggatgcta
    40741 tttcgagaaa acagttgcag gttctgacaa aagaattgca taaagagttt gggatgacaa
    40801 cgatttttgt aacccatgat acggatgaag ccttgaagtt ggcggaccgt attgctgtct
    40861 tgcaggatgg agaaattcgc caggtagcga atcccgagac aattttaaaa gtgcctgcaa
    40921 cagactttgt agcagacttg tttggaggta gtgttcatga ctaatttaat tgcaactttt
    40981 caggatcgtt ttagtgattg gttgacagct ctatctcaac atttgcagtt gtcacttttg
    41041 accttgttac tagctatttt gcttgcgatt cccttggctg tttttcttcg ctatcatgag
    41101 aagctggccg actgggtctt gcagattgcc ggtattttcc agaccatccc gtctctggcc
    41161 ttgttggggc tctttatccc tttgatggga attgggacct tgccggcttt gacagctcta
    41221 gtgatttatg cgattttccc tattttgcaa aatactatca ctgggctgaa gggaattgat
    41281 ccgaacctgc aagaggctgg gattgccttt gggatgacca gatgggaacg tctcaagaaa
    41341 tttgaaattc cactcgccat gcctgttatc atgtctggga ttcggacggc agctgttttg
    41401 attatcggta cggcaacctt ggcggccttg attggagcag ggggactggg ttcctttatc
    41461 ctgttgggaa ttgaccgtaa taatgccagt ttgattttga ttggggcact ttcttctgca
    41521 gtgcttgcta tcgcctttaa cttcctacta aaagtaatgg aaaaagcaaa attacggacg
    41581 attttctcag gttttgcctt ggtggcttta ttactgggtc tgtcttatag tccagctctt
    41641 ttggttcaaa aagagaagga aaacttggtt attgctggga aaataggtcc agaaccagaa
    41701 attttggcca atatgtataa gttgctgatt gaagaaaata ccagcatgac tgcgactgtt
    41761 aaaccgaatt ttgggaagac aagcttcctt tatgaagctc tgaaaaaagg cgatattgac
    41821 atctatcctg aatttactgg tacggtgact gaaagtttgc ttcaaccatc acccaaggtg
    41881 agtcatgaac cagaacaggt ttatcaggtg gcgcgtgatg gcattgctaa gcaggatcat
    41941 ctagcctatc tcaaacccat gtcttatcaa aacacctatg ctgtagctgt ttcgaaaaag
    42001 attgctcaag aatatggctt gaagaccatt tcagacttga aaaaagtgga agggcagttg
    42061 aaggcaggtt ttacactcga gtttaacgac cgtgaagatg gaaataaggg cttgcaatca
    42121 atgtatggtc tcaatcttaa tgtagcgacc atggagccag cccttcgcta tcaggctatt
    42181 cagtcaggtg atattcaaat tacggacgcc tattcgactg atgcggaatt ggagcgttat
    42241 gatttacagg tcttggaaga tgacaagcaa ctcttcccac cttatcaagg ggctccactc
    42301 atgaaagaag ctcttctcaa gaaacaccca gagttggaaa gagttcttaa tacattggct
    42361 ggtaagatta cagaaagcca gatgagccag ctcaactacc aagtcggtgt tgaaggcaag
    42421 tcagcaaagc aagtagccaa ggagtttctc caagaacaag gtttgttgaa gaaatgatgg
    42481 gacagagact ttaaacatac tgaactcaac ttccttaaac gactagataa aagaatgctc
    42541 agacaatttc gtctgggctt ttttgatgtg agaatgactg aatgtcactt aaaatggaaa
    42601 gaaagagaaa attttcatga ttttctaaaa ttttactgta aagacagttg actattcata
    42661 aaataggtgt ataatatttt gtgaactact taacaaataa ggggtttcag ctcatgtcga
    42721 ctaaggttag aaatcttgtg tataggcagt tcattatcta atagagcgtt gcgtccgaaa
    42781 gtctatccag acacggctct ttaaaaacaa aaggagaaat gatgcatact tatttgcaaa
    42841 agaaaattga aaatatcaaa acaaccctag gtgaaatgtc aggtggttac cgtcgtatgg
    42901 ttgcggctat ggctgattta ggattttcag gaactatgaa ggctatctgg gatgacctct
    42961 ttgcccatcg tagttttgcc cagtggattt atttgctggt tttaggaagt tttcctctct
    43021 ggctggagtt ggtttacgaa catcgtattg ttgactggat tgggatgatt tgtagcttga
    43081 cagggattat ctgtgtaatc tttgtatcgg aaggtcgagc aagtaattat ctttttggct
    43141 tgattaactc tgttatttac cttattttgg ccctacagaa aggcttttat ggtgaggtgc
    43201 tgacgacact ttacttcaca gtcatgcagc caattggact tctagtttgg atttatcagg
    43261 cacagtttaa gaaggaaaag caggagtttg tcgcgcgtaa actggacggc aagggctgga
    43321 caaagtatct ttccattagt gtgctttggt ggttggcctt tggcttcatt tatcagtcta
    43381 ttggtgccaa tcgtccctat cgtgattcaa tcacagatgc aaccaatggg gtagggcaaa
    43441 tcctcatgac agctgtttac cgtgaacagt ggatattctg ggcggctacc aatgtctttt
    43501 caatctatct ctggtgggga gaaagcctgc aaattcaagg gaaatatcta atttatctca
    43561 ttaacagtct agttggttgg tatcaatgga gcaaggcagc taagcagaat actgatttac
    43621 ttaactagga aaagatgttt gaaagtgctg ttttgagatt tcgattaaaa cagatatagt
    43681 tgataatcaa ggatttatag tatgaaaaag aggatcggcg ggtcctcttt tgttgttgaa
    43741 aagataaaaa actcagtaac ctagaaataa gacaactgaa gctttactct atattcaatt
    43801 tttaggaatg agaaggtcta gataaaattg gacaacttcc tggtctgtga aatcttgacc
    43861 ttttttgagc caccaggtca atgtttcgat aaagttagtt acgacaaaat gttggaggta
    43921 agaagtaggt agacttgggt gagtttgctt caaatcagca gccagcatgg gatagacgtg
    43981 gtgctcaagc tctctctgta gttgacggag gaaatagtca tttttggaaa atagcagact
    44041 ggcgatatgg tcttggtttt tctgaaaatg gagaaagagg tgggcgaggt agtcctcggt
    44101 tgaaatggct tgctctcttt caaaaagatg atggaagagg tagcggcaga gctgatccag
    44161 aagaagctct ttactctcat agtgacagta aaaggtggat cgtcccacat ctgcgagatc
    44221 aatgatatcc tgaacagtag tggtctcgta gtccttagca ttcaaaagtt gtataaaagc
    44281 ttgatagatg gcttttttgg ttttgctgat acggcggtca atgttagtca tatggacact
    44341 tgaggcaaat tgttcagaac tgaataaagc tgacgttttg cttctatcct ttctttgagt
    44401 tttagtggat aatgataatg aacaaggtgt tcataaatct attataacaa aggaatgaga
    44461 aatatgaagg caaaatatgc tgtttgggtg gcttttttct taaatttgac ttatgccatt
    44521 gttgagttta ttgcaggtgg agtatttggt tctagcgctg ttcttgctga ctctgtgcat
    44581 gacttgggag atgtgattgc aattggaata tcagcttttc tagaaacaat ctccaatcgt
    44641 gaagaagaca atcagtacac cttgggctat aagcggttta gcctgctagg agccgtggta
    44701 acagctgtga ttctcgtaac aggctctgtt ctagtcattt tggaaaatgt cacgaagatt
    44761 ttgcatccgc aaccagtcaa tgatgagggg attctctggt taggaattat tgcgattact
    44821 atcaatctgt tagcgagtct ggtggttggt aagggaaaga caaagaatga gtctattctg
    44881 agtctgcatt ttctggaaga tacgctaggg tgggtagctg ttatcctgat ggcgattgtt
    44941 cttcgattta cggactggta tatcctagat cctcttttgt cccttgtcat ttctttcttt
    45001 attctttcaa aagcccttcc acgtttttgg tctacactca agattttctt ggatgctgtg
    45061 ccagaaggtc ttgatatcaa gcaagtaaag agtgacctgg agcgattgga caatgtggcc
    45121 agccttaatc agcttaatct ctggactatg gatgctttgg aaaaaaatgc cattgtccat
    45181 gtttgtctaa aagaaatgga acatatggaa acttgtaaag agtctattcg aattttccta
    45241 aaagattgtg gttttcaaaa tattaccatt gaaattgatg ctgacctaga aactcaccaa
    45301 acccataagc gaaaggtgtg tgacttggaa cggagttatg agcatcaaca ttagaaaaaa
    45361 gtgaaaaata cttgggtact atcttatttg gaatagagta atttctttat tatttaaata
    45421 tttcaaaaat tggtaagaga agagcattgt ataaactcca gatatatgat tgttaatgat
    45481 aaaaattttt cgattagata caaaatgctt gacttggagt caactcaaag ttatataata
    45541 agataagtga gttagaatag cgtgaattca gtgaatgaaa tgagaggagg ttagcgtgtg
    45601 aatattaaat ctgccagtga tttgttggga atttcagcgg atacgattcg gtattatgaa
    45661 cgggttggtc ttgtgccacc gattactcgt actgctactg ggattcgtga ttttcaagat
    45721 caggatatcg aagcgctgga atttattaag tgttttcgtt cggcgggtgt ctctgtagat
    45781 agtttagttg actatatgtc gctctaccaa aagggagatg aaacgagaga ggagaggctt
    45841 ggtattttag aagaggaaaa gcaaaaatta gaggagcgct tgtctcagct acagacagct
    45901 ttaaatcgtt taaatctcaa aattaaactt tataaggaag gaaaatttta aatgaaatca
    45961 gcagtatata caaaggcagg tcaggttgga cttgctagca ttgaacgtcc gcaaataata
    46021 gaagcggatg atgtgattat tcgtgtggtt cgtgcgtgcg tttgtggttc agatttatgg
    46081 aggtaccgta atccagaaac gaaagctgga cacaaaaata gtggacacga agcgattggg
    46141 attgttgaag aagctgggga agccattacg acggtgaaac caggtgattt tgtgattgtc
    46201 ccttttacac atggatgtgg tgagtgtgat gcctgtcttg ctggatttga cggttcttgc
    46261 gacaatcata ttggcaataa tttggggggt gattttcagg cagaatatat tcgcttccac
    46321 tatgcaaact gggcgctggt taaaatccct ggtcaacctt ctgactatac agaagggatg
    46381 ctcaagtccc ttttgactct tgcagatgtc atgccgacag gctatcatgc ggcgcgtgtt
    46441 gcaaatgttc aaaaagggga caaggttgtt gttatcggtg atggggctgt tggtcaatgt
    46501 gctgtcatcg cggctaagat gcgtggagca tcacaaatta tccttatgag ccgtcatgaa
    46561 gaccgtcaaa agatggctat ggagtcaggt gcgacagctg ttgttgcaga acgtggtcaa
    46621 gaaggaatta ccaaggtgcg tgaaatcctc ggtggaggag cagatgcagc acttgaatgt
    46681 gttggtacgg aggctgctat agaacaggcg ctaggtgttc ttcataatgg agggcgtatg
    46741 ggctttgtag gagtcccaca ctataataat cgtgctcttg gttcgacatt tatgcaaaat
    46801 atctctgtag caggtggggc agcttctgct acaacatacg ataagcaatt tttactaaaa
    46861 gccgtccttg atggtgatat caatccaggt cgcgtcttta cttcaagtta taaactggaa
    46921 gatatcgacc aagcctataa agatatggat gaacgtaaga caattaagtc tatgattgta
    46981 atcgaataaa aaacgaatag gagttttaga actctattcg ttttttatgt tatcctattc
    47041 ttgatttagg gtactttctc ttaatgtcag tctggttccc agcatggtca ggctagggat
    47101 tttccgaccg tggaggactt ccttgttaag aatatccata cctgctcggc ccatttcttc
    47161 agtataaact gtaatactag agaggggagg atagacctgt ttggtcagac tagtgtcgtt
    47221 aaaggaaatg aggctgacgc gatctggcag gctgattcca gcttcttgga gggcaccgat
    47281 agctaaacta tcgctggctg cgaaaaatgc tggcggaagt tggtctccca agctctgaat
    47341 ggcctccttc attaagtcat agccagactg ggcagtaaat cttccttgaa agaccagttc
    47401 atcatgatag attcccctcg cttgactata gtttttgaag ttttctagac gcttgtcctg
    47461 aatgatttct tcttggtctg ttgtttcttc aaggcctgtt agaatcccga tacggtccat
    47521 tccttgactg aggaaataat cgacaacctg tttcatagca gtgtaaaaat ccgtgataat
    47581 acaggtatgt cccagggaaa gtgtatcgct gtctagaaat acaagaggct tttggtattc
    47641 ttcaaaggca gaaatctgag ctcgactaaa ctttccgatg cagagaatcc caatcacttc
    47701 ctcgcttagg gtaaaagggt ggtcattaaa atagcgcaag atatcatagt ccaactcttg
    47761 ggctcttttt tctattccta ggcgaatctg gtagtagtag aggtcgtcca gctccccttg
    47821 ttcgctgacc cattggataa tggcaatctt ttgcttgggt ttgtgggact cgcctgtctt
    47881 gaggtgcttg gtgtagccca gctcttcagc aacggttaaa atacggtgtc tggtttcttc
    47941 tgtaacagat aggctctggt cgcggttgag gacgcgggat acggtcgcga tagagacaga
    48001 ggctagctgt gcaatgtctt ttaaggtagc cataaatcct ccttgattag gttagtatat
    48061 catgtttttc ttctttttac tgatatttta ctaaaatttt agtaaaaagg attgaccttg
    48121 gaaaattcct tggatataat agaaagaaaa cgattacacg ttaagatggc ttaacggaca
    48181 gtcaaaggag aattcatatg gcacaacatc ttactactga agcccttcgc aaagactttc
    48241 ttgctgtttt tggtcaagaa gcagatcaaa ccttcttttc accaggccgc attaatttga
    48301 ttggtgaaca cacagactac aacggtgggc acgtttttcc tgctgctatt tccttgggaa
    48361 cttacggtgc agctcgtaag cgtgacgacc aagtcttgcg tttctactca gctaactttg
    48421 aggacaaggg cattatcgaa gtgcctctcg ctgacctcaa gtttgaaaaa gagcacaact
    48481 ggaccaatta tccaaaaggt gtccttcatt tcttgcaaga agctgggcac gtgattgaca
    48541 aaggttttga tttttatgtt tatggaaata ttccaaatgg tgctggcttg tcttcttctg
    48601 catccttgga actcttgaca ggagtcgtgg ctgagcatct ctttgattta aaattagagc
    48661 gtctcgattt ggttaaaatc ggcaaacaaa cagaaaacaa ctttatcgga gtaaactctg
    48721 gcattatgga ccagtttgct attggtatgg gggcagacca acgtgctatt tacctagata
    48781 ctaatacttt agaatacgac ttggtgccac ttgatttgaa ggacaatgtc gttgttatca
    48841 tgaacaccaa caaacgccgt gaattggcgg actctaaata caatgaacgt cgtgctgagt
    48901 gtgaaaaagc agtggaagaa ttgcaagttt ccttggatat tcagactctg ggtgaattgg
    48961 acgagtgggc cgttgaccaa tatagctatc tgattaaaga tgaaaatcgt ttgaaacgtg
    49021 ctcgccatgc tgtgcttgaa aaccaacgta ccctcaaagc tcaagtagca ctccaagcag
    49081 gagatttgga aacatttgga cgcttgatga atgcgtcaca cgtttctctg gagcatgatt
    49141 atgaagtaac tggtttggaa ttggataccc ttgttcacac agcttgggca caagaaggag
    49201 ttctcggtgc tcgtatgaca ggggctggtt ttggtggctg tgccattgcc ttggttcaaa
    49261 aagatactgt tgaggccttt aaggaagctg taggcaaaca ctacgaggaa gtagttggat
    49321 acgctccaag cttctatatc gctgaagttg caggtggcac tcgcgtcctt gactagtcaa
    49381 aaggaggctc tatagtgacc ttagtaaata aatttgtaac acatgtcatt tctgaaagct
    49441 catttgagga aatggatcga atctatctga ccaatcgtgt tttggcacga gtgggagaag
    49501 gtgttttgga agttgagacc aatctggata aattgattga cctcaaggac cagctggttg
    49561 aagaagccgt tcgattagag acgattgagg atagtcagac tgcgcgtgaa atccttggtg
    49621 ctgaactgat ggatttggtg actccttgtc caagtcaggt caatcgtgat ttttgggcaa
    49681 cctacgccca ctctccagaa caagcgatag aggattttta ccaactcagt cagaaaaatg
    49741 actacatcaa actcaaggcc attgctagaa atatcgctta tcgtgttcca tctgactacg
    49801 gagaacttga aattaccatc aatctctcta agcctgaaaa agatcccaaa gagattgtgg
    49861 cagccaagtt ggtgcaagct agtaattatc ctcagtgtca gctttgtcta gagaatgagg
    49921 gctaccatgg tcgagttaac cacccagctc gtagcaatca ccgtattatc cgttttgaaa
    49981 tggttggtca ggaatggggt ttccagtatt cgccctatgc ttactttaat gagcattgta
    50041 tctttttaga tggccagcat cgtcccatgg ccattagtcg tcagagtttt gaacgtctgt
    50101 tggctatcgt agaccagttt ccaggatatt ttgctggatc taatgccgac ctgccgattg
    50161 tggggggctc tattctaact catgatcatt atcagggagg ccgtcacgta tttcctatgg
    50221 aattggctcc cttgcaaaag gccttccgat ttgctggttt tgagcaggtc aaggctggaa
    50281 ttgtcaagtg gcccatgtct gtcctacgtt tgacttcgga ttccaaagag gatttgatca
    50341 atttggctga taagattttg caggaatggc gccagtattc agatcctgca gtgcagattt
    50401 tggcagagac agacaggaca ccgcatcaca ctatcacacc cattgcccgc aaacgcgatg
    50461 gacagtttga gttggacttg gtcttgcgag acaatcagac ttcagcagag tatcctgatg
    50521 gtatctatca tccccacaag gatgtccaac atatcaagaa ggaaaatatc ggcttgattg
    50581 aggtcatggg cttggcaatc ttgccaccac gtctgaaaga agaagtggag caagtcgcta
    50641 gctatcttgt aggagaagct gttacagttg ccgattatca tcaggagtgg gcagaccaac
    50701 tcaaatccca acatccagac ctaacggata aagaaaaagc ccttgcaatc gtcaaggact
    50761 ctgtgggtgc tatctttgcg cgtgtacttg aggatgcagg agtctacaag cagacagaac
    50821 aagggcagac agcctttatg cgctttgtgg aacaggtcgg aattttacta gactaggagc
    50881 tttctccttg cacagtccta taaaaagtag tatcatagtg tcgtttgaaa tatcaggagg
    50941 aaacgatgaa agactttcat tttgacgcta tatctgcctt tgaaaattac gaaattgaaa
    51001 aaatgagaga tggtcacgtt gtggtgacga ccaaagtagt gaactcgtcg ctcaactact
    51061 atggcaatgc ccatggtggc tatctattca cgctatgtga ccaaatcagt ggtttggtgg
    51121 ttatctcgct gggacttgat ggggtgacac tccaatcctc tatcaactac cttaaggcag
    51181 gaaaactcga cgatgtattg accattaaag gagaatgtgt ccatcaaggt cgtacaactt
    51241 gtgtaatgga tgtggatatc accaatcaag agggcagaaa tgtctgcaaa gcaaccttta
    51301 ccatgtttgt cacaggccaa aggtctgagg aaagacgggt aaggatataa agaaaggtga
    51361 gtgggataaa tttatctcac tcatttttaa tttatgagat atgttagaag gaaatataaa
    51421 agagagtgat gaaatacaaa tgtaataggt acatatatgt ggtatactag ttatagtaga
    51481 ttttaattgc ttatatcgag tgagttctca tattagtgag gaatttttaa atggaattac
    51541 attttaattt agaattagta gaaacctata aaagtaatag ccaaaaggca cgtatcttaa
    51601 cggaggattg ggtttatcgt cagtcttatt gccctaattg tgggaacaat cccttaaatc
    51661 attttgaaaa taatcggcct gtagcagatt tttactgtaa tcattgtagt gaggagtttg
    51721 aactaaagag caaaaaagga aatttttcat caacaatcaa tgatggtgct tatgcaacga
    51781 tgatgaagcg tgtgcaggca gataataatc ctaatttctt ttttttaact tacacaaaaa
    51841 attttgaggt aaataacttt cttgtccttc cgaagcaatt tgttacaccg aaatcgatta
    51901 ttcaaagaaa accacttgca ccaactgcta gacgagcagg ttggattggt tgtaacattg
    51961 atttatcaca agtaccttct aaaggaagga tatttcttgt gcaagatgga caagttagag
    52021 atccagaaaa agttacaaaa gaatttaagc aaggtttatt tttaaggaag agctctctgt
    52081 catcaagagg ttggacaata gaaattctaa attgtataga taagatagag ggttcagaat
    52141 ttacccttga agatatgtat cgttttgaaa gtgacctaaa aaatatcttt gttaagaaca
    52201 atcatatcaa agaaaagatt aggcaacagc ttcaaatatt aagagacaaa gaaataatag
    52261 aatttaaagg tagaggaaag tatcggaaat tatgaaaacg aaacaacttg ttgcatcaga
    52321 agaggtgtat gatttcttaa aagtcatctg gcctgattat gaaactgaaa gccgttacga
    52381 taacctaagt ttaatcgtct gtaccttatc agatcccgat tgtgtgagat ggttatctga
    52441 aaatatgaaa tttggtgacg aaaaacaact agctttgatg aaggaaaaat atgggtggga
    52501 agtaggagat aaattgccag agtggctaca tagctcctat catagattat tgttaatagg
    52561 tgaattattg gaaagcaatc taaaactgaa aaagtataca gtagaaatta cagaaacttt
    52621 atcacgttta gtaagtatag aggctgaaaa tccagatgaa gccgaacgac ttgtaagaga
    52681 aaagtataag agttgtgaaa ttgttcttga tgcagatgat tttcaggact atgacactag
    52741 catatatgaa taggtagatg tttttatttt gtcaacaaaa aagaggctcg cacctctttt
    52801 tcttatttct ttttatgatt taatacggca ttgaggacaa tagcgagtag gctggctacg
    52861 acgattccgt ttgagaagaa catttggaag gctgtcggca tgctgacaaa gagattactg
    52921 ttgttgagac cgacacctgc agcgattgaa acagctgcga taaggaagtt gtgttcattg
    52981 ttagcaaagt caacacgggc gaggatttgc atcccttgaa ttgatacaaa accaaacatt
    53041 accagcatgg caccaccgag gacggagctt ggaatgattt gggcaagggc gccaaactta
    53101 ggaagcagtc caaggagaac caggaaacca gctgcgtagt agattggcag gcgttttttg
    53161 atgcctgaca atttaaccaa accaacgttt tgtgaaaatc cggtgtaagg gaaggtgtta
    53221 aagattcctc cgagaagtac ggccaaacct tctgcgcggt atccgttgcg aaggcgcgtg
    53281 ctgtcgattg gatcctttgt gatatcagac aaggccagat aaacaccagt tgactcaacc
    53341 atagacaccg ttgcgatgat acacatcatg acaatagatg agatttcaaa ggttggcatc
    53401 ccaaagtaga gtggagttgg gacatggaca agtggagcta ccgcaacagg agagaagtcc
    53461 accaagccca tagtagcagc aatggcagtt ccaacaacca gaccaatcaa aatagagata
    53521 gacttgataa atcctttggt aaagatgttg atcaagagga taatcagaac agtaatagct
    53581 gcaagcaaga gactttgacc agttggctct ggaacgttat ttcccatatt tccaatagcg
    53641 acagggatca aggttaaacc aatcgtggta ataacagatc ctgttacgat agatgggaag
    53701 agattggcta cttttgagaa gatgcctgaa acaagaacca cgtaaatccc agatgcgata
    53761 agggcaccaa acatagcgcc actaccatgg ctttgcccaa tcataatcaa gggagcgacc
    53821 gactggaatg caactccaag aacgactggg agtccaatcc caaagtattt gttgagttgg
    53881 agttggagga aggttgccac cccacacatg aagatatctg tagaaatcag gtaggtcaac
    53941 tgctcagctg aatagccaag ggctgtcgca atcatgatgg gaaccaggat agatcctgag
    54001 tacatggcta gtaagtgctg caagccaaga acggctgctt gcgagtgttt ttcttgagtt
    54061 tgcattagag atctgcctcc ttaaatacga cttgaccatt ttcaaaacaa tccaaacgag
    54121 caagtgatag gacagggtag cctgcttttt caagcaaatc acgaccatct tggaaggatt
    54181 tctcaatcac gataccgata gcttggactg tggcaccggc ctgttcgatg atttgaatca
    54241 agcctttagc agttaagatg ccttcgttca tggtgatgtt cttagctttt ttggcgaaaa
    54301 tcatgggaac gtttaaggct tcagctgtaa aaacggctgg ggcaataccc gacgcttcaa
    54361 tggttacgac cttggtaatg ccagtagtag caaatttttc cgcaaaaacc ttaccaatct
    54421 ctcgcatcaa gctaaagtca acttggtggg ttaaaaagga atctaccttg aggatgttat
    54481 cacccaagat atgcccatcc ttgaggatgc gctcttctaa taatttcata agacctccta
    54541 aagtctaaaa gttaatttac ttgttgttta aatatttcta tagtgatccc ttttgctaat
    54601 actatatatt tgataaaact attacgagcg aagcgagtct tatcaaatat ttcccgttgt
    54661 agtggtatca tagacaataa tcttgttatt gtctatgacg ggatttttga gagtaaaata
    54721 gttcggggaa ctattttagc ctaagcctag aaatgaaaga gctaggggct caaaaattag
    54781 ggatgaaatt ccctggattc ctgaaattat tcacaggata atttcacctc ccgtccgcac
    54841 taattaaggg aaatattaaa aaaagaccta cttaatctct aagtaagtcc cctaaataga
    54901 catggcaaaa acggccatat ctcactgctg acttacttat tgttaggtgt tccggcacct
    54961 tgtagaaacg tcgtgccaat tcacgacata aacaagtaaa acgatattca attttaaata
    55021 ggcttgagcc aatgttttta ttttacacta aataacttta gaaatcaact attttgttag
    55081 tgttttggtt taaaaaacga acaaaaagaa gagagggtga acaaaaactc cattgtaagc
    55141 taacagttat actaaatgaa aatcaaagag caaactagga agctatccac aacctcaaaa
    55201 cactgttttg aggttgtgga tagaattgac agagccagta tcatatacct acggtaaggc
    55261 gacgttgacg tggcttgaag agattttcga agagtattag aagatttttc catcataaaa
    55321 ggcatactat caagctttta gacacctgac aatatgcctt tttctaactt taaagacttt
    55381 tcccaatttt tattattcta ctcgctaaat cttaaaaaat agccatctgg atccaaaact
    55441 gcaaatttat gaggatagat atagggatca ctgacacgaa actttctttt ggtcaaggga
    55501 cgataaatag gatagtttgc cttcatcact ctttaataga gttttgaaac atcctttatg
    55561 ccaaaggaga gattgactcc acgaccaaag ggataggtca gttcagctag ttgatccttt
    55621 gttccctcct ctaacattag ttgacactct tcaagagaaa gagaaagttt tcttctggac
    55681 gttggtattc aatcctaaaa cccagtaaac cacagtagaa ggaccgggac tgttcgatat
    55741 tcgatacaag caactcggga atgaccgcat tgtagtccat atagaaaatc cttacaagtc
    55801 aatttccaag acaatcggtg tatggtcttg gcgagcacct gagtcaatca tatcagattt
    55861 agtgaccttg tcagcgatac ggttacttgt gagccagtag tcgattctcc agcctgtatt
    55921 gttgatttta gaagttttgc tgcgttgtgc ccaccaagtg tagcgttcag gaacatcgcc
    55981 atgaacatgg cggaaggtgt ctgtaaatcc agttgccaaa aggttggtaa atccagcacg
    56041 ttcctcgtca gtaaatccag gtgaacggcg gttgctagca ggatttgcaa ggtcgatttc
    56101 attgtgggct acgttgtagt caccggtcgc aaggactggt ttttctttgt ctagttcagc
    56161 caaatactca gcatatttgg catcccagac ttggcgttct tccaagcgtt tgagaccgtc
    56221 accagcgttt ggagtgtaaa cttgggttac gaaaaatgca tcaaattcta gagtgatgat
    56281 acgaccttcc aagtccatgg tagaaggggc accgatttct gggaagctga tagtaggtgt
    56341 aagttctttc ttataaagga acatggttcc agcatagcct ttacgggcag gctcttggga
    56401 agagcgccac gtgttttcgt agcctgggaa gagttcttct aaaatttcta agtgtttctt
    56461 tgtaggccct ttggcagaaa gcttggtttc ttggatagca atgatatcag cattttcagc
    56521 gaccaaggtt tgtaggactt cttgggacaa tttggcacga gctgagtcac tagttagggc
    56581 agcgtttagg gaatcaatat tccatgagat aagtttcata aagttacctt ttcattcaga
    56641 ttatagattt tattatacca aaaaaagatc tatttcccca acgtatagtt tgaaaaatta
    56701 ctctctttcg tttataatta agaatgattt tatgaaaggg agtgaaaata catgaaattc
    56761 tactcttatg actatgtact cagccaaatc ggtcagcaaa atggtatcat ggttggcttt
    56821 gggattgttc tattagctgt gacagttttt tttgctttta aggcatacca tgataaaaag
    56881 ggaaccgaat ttcgtgagtt ggtcatgatt tcagctctgg ccttatttag ctctgctttt
    56941 ggtcagcatc acgacttatc aaaacaatca agtttctaac aataaatttc aaacttcact
    57001 tcatttcatc gaggttgttt ccaaagattt gggagtagac aagtcagaag tctatgttaa
    57061 tacttccaca aacacagatg gcgcacttat caaggtggga gatcgctatt atcgtgccct
    57121 aaacggaagt gagccagaca agtacctgtt agagaaagtc gaattgtata agacagacgc
    57181 aattgaactg gtggatgtga acaaatgaca cttaattata tcgaaatttt aatcaaactg
    57241 gtcttgactc tcaaatggct caacaacaat gttcactttg tgaaacgttt gattgatggt
    57301 aagccaactc tccttatcaa aaatgggaat attgacccat aaacctgtcg ttcagttggt
    57361 ttgtctgcat cggatgtagc cctcaaactt cgtagccaag ggattttcca gatgaagcaa
    57421 gtcaaacgag ctgtgcaaga gcaaaatggg caactcatcg ttgtgcaaat gggagatgaa
    57481 aatcctaagt atccagttgt gactgacggt gtgattcaag tagatgtctt ggaatcgatt
    57541 ggtcgtagcg aagagtggtt gcttgataac ctcagtaaac aagggcatga caatgtagcc
    57601 aatatcttta ttgctgaata tgacaagggt gctgttacag tcgtaactta tgaaaaagaa
    57661 aaacctgggg tcttgtactc ttcgaaaatc tcttcaaacc gcgtcaacgt cgccttgccg
    57721 tatgtaggtt actgacttcg tcagttctat ctacaacctc aaagcagtgc tttgagcagc
    57781 ctgcggctag tttcctagtt tgctctttga ttttcattga gtattggcct caggtttcca
    57841 tttgcaatca gaaagggatt ttatgtccat tattcaaaaa ctttggtggt ttttcaagtt
    57901 agaaaaacgc cgttatctag tcggaattgt ggccctggtc ttggtttccg tcctcaatct
    57961 cattcctcct atggttatgg ggcgggttat tgatgccatc acatcggggc aattaaccca
    58021 gcaggacctc cttcttagcc tattttactt gctacttgca gcctttggta tgtactattt
    58081 gcgctatgtg tggcgtatgt atatccttgg gacctcttat tgcttgggac agatcatgcg
    58141 gtctcgcttg tttaagcatt tcacaaaaat gtcgtcagcc ttttatcaaa cctatcggac
    58201 gggtgatctg atggcacacg caaccaatga tataaatgcc ttgactcgtt tagcaggtgg
    58261 cggtgtcatg tctgcggtgg atgcctctat cacggctctg gtgactttgt tgaccatgct
    58321 ctttagcatc tcatggcaga tgactcttgt tgccattctc cccctacctt tcatggccta
    58381 tgcgactagt cgcctaggga gaaagactca taaggccttt ggcgaatccc aagctgcttt
    58441 ttctgaactc aataacaagg tacaggagtc cgtatcaggt atcaaagtga ccaagtcttt
    58501 cggttatcag gcagacgagt tgaagtcttt tcaggcagtc aatgaattaa ccttccaaaa
    58561 gaacctgcaa accatgaaat atgatagtct ctttgaccct atggttctct tgtttgttgg
    58621 ttcgtcctat gttttaacgc ttttggttgg ctccttggtg gttcaggaag ggcagattac
    58681 agttgggaat ctagtcacct ttatcagcta tttggatatg ctggtctggc ctcttatggc
    58741 catcggcttc ctctttaata ctactcagcg agggaaggtt tcttaccagc ggattgaaaa
    58801 tcttttgtct caggaatctc ctgtacaaga cccggagttt cctctggatg gtattgaaaa
    58861 tgggcgtttg gagtatgcca ttgacagctt tgcttttgaa aatgaggaaa cactgacgga
    58921 tattcacttt agtttggcaa aagggcaaac actgggcttg gttgggcaga caggctctgg
    58981 gaaaacgtcc ttaatcaagc tcctcttgcg tgaatacgat gtggataagg gtgccattta
    59041 tctaaacggt cacgatattc gggactatcg tctgacagac cttcgcagtc tcatgggcta
    59101 tgttcctcag gaccagtttc tttttgcgac ttcaatccta gacaatatcc gctttggcaa
    59161 tcctaacttg cccctttcag cggtcgagga agctactaag ctagcccggg tttaccaaga
    59221 tattgtagac atgcctcaag gatttgatac gctgattggt gaaaaaggag tcagtctttc
    59281 tggtggtcaa aagcaacggt tggctatgag tcgggctatg attttagacc ctgatatctt
    59341 gattttggat gattccttat ccgccgtaga tgccaagaca gagtatgcga ttatcgacaa
    59401 cctcaaggag acgcgaaagg acaagacaac cattatcact gcccatcgcc tcagtgctgt
    59461 tgtccatgca gattttattt tagttctaca aaatggtcaa attatcgaac gaggcacgca
    59521 cgaagacttg ctagcgatgg atggctggta tgcccaaacc taccagtctc agcagttgga
    59581 aatgaaagga gaagaagatg cagaataaac aagaacaatg ggctgtattg aagcgcttga
    59641 tgtcttatct caagccttat ggactcctga cctttttggc actcagtttt ctcctagcga
    59701 cgacggtcat taaaagtgtc atacccctcg tggcttccca ctttatcgac cagtatctca
    59761 gcaatcttaa ccaactagcc gttaccgttt tgctggtcta ctatggtctc tacatcctac
    59821 aaactgtagt tcagtatgtg ggcaatcttc tctttgcgcg cgtgtcttac agtattgtta
    59881 gggatattcg tcgggatgcc tttgccaata tggagaaact gggcatgtct tactttgaca
    59941 agacgccagc aggttctatc gtttctcgtt tgaccaacga taccgagacg attagtgata
    60001 tgttttctgg gattttatcc agctttatct cagcagtttt tatctttctg acaacccttt
    60061 ataccatgtt ggtgctggat tttcgtttga cggctttagt cttgctcttt cttcctttga
    60121 ttttcctttt ggtcaatctc tatcgaaaaa agtcagtgaa aatcatcgag aaaaccagaa
    60181 gtctcttgtc agatatcaat agtaagctgg cagagaatat cgagggaatc aggattattc
    60241 aggcctttaa tcaagagaag cgcctgcagg cagaatttga tgaaatcaac caagaacact
    60301 tggtctacgc caaccgttct gtagccttgg atgccctctt tttgagacct gccatgagtt
    60361 tgctgaaact tctaggctat gcagtcttga tggcctactt tggctaccgt ggtttttcta
    60421 tcgggataac ggccgggacc atgtatgcct ttatccagta catcaaccgc ctttttgacc
    60481 ccttgattga ggtgacgcaa aacttttcaa ctctgcaaac gtctatggtt tctgcaggcc
    60541 gtgtctttgc cttaatcgat gagagaacct atgagcctct tcaagaaaat gggcaagcca
    60601 aagtcaaaga aggcaatatc cgttttgaac atgtgtgttt ctcgtatgac ggtaaacatc
    60661 cgattctgga tgacatttct ttctctgtta ataagggtga aaccattgcc tttgtaggtc
    60721 atacaggttc agggaaatcg tctattatca atgtcctcat gcgcttttat gaattccagt
    60781 cagggagagt tctcttggat gatgtggata tcagggattt cagtcaagaa gagctgagaa
    60841 aaaacatcgg tttggtttta caggaaccct tcctctatca tggaactatt aagtccaata
    60901 tcgccatgta ccaagaaatc agtgatgagc aggttcaggc tgcggcagcc tttgtggatg
    60961 cagattcctt tattcaagaa cttcctcagg ggtacgactc ccctgtttcc gagcgtggtt
    61021 cgagcttctc tactgggcag cgccagcttc ttgcctttgc tagaacagtc gccagccagc
    61081 ctaaaatcct gattttggat gaagcgacag ccaatattga ctctgaaaca gaaagcttgg
    61141 ttcaagcttc tctggcgaag atgagacagg gccgaacaac tattgctatc gctcaccgcc
    61201 tttctactat tcaagacgcc aactgcatct atgtcttgga taagggacgc attatcgaga
    61261 gtggaaccca tgaggaactc ttgactctgg gaggaaccta tcacaagatg tatagtttgc
    61321 aggcaggggc catggcctat actctttgaa aatctcttta aaccatgtca gctttatctg
    61381 caatctcaaa gctgtacttt gattttcatt gagtactaga aggaaatcct tcaaattaca
    61441 gatttctttc accgcctttt ccattttgtg gtataatgaa aaatgttgac aaatagtata
    61501 ataaaaacaa aggagaacag tatgctgaaa tgggaagact tgcctgtgga aatgaaatca
    61561 agcgaggttg agtcttacta ccagcttgtc tctaaaagga agggttcgct gattttcaag
    61621 cgttgcttgg actggttttt ggccttggtc ttactggttc taacctctcc catctttctc
    61681 atcttgagca tttggatcaa gttggatagc aaggggccag tgatttacaa gcaagagcgt
    61741 gtgacccagt acaaccgtcg gttcaagatt tggaagttcc gtaccatggt gacggatgcg
    61801 gataaaaaag gaagtctggt gacttctgct aacgatagcc gtattaccaa ggttggaaat
    61861 ttcatccgac gtgtccgttt ggacgaactg cctcagttgg tcaatgtcct taaaggtgag
    61921 atgtcctttg tcggtacacg acctgaagtg ccacgttata cagagcagta tagccctgaa
    61981 atgatggcaa ccttgctctt gcaagcagga attacctctc cagccagcat caactacaag
    62041 gatgaggaca ccatcatcag tcaaatgacg gagaaaggtc tgtcagttga tcaggcctat
    62101 gtggagcatg ttcttcctga aaagatgcgc tataacctcg cctatctccg agagtttagt
    62161 ttctttgggg acatcaaaat catgtttcaa accgtgtttg aggtactaaa ataaagtagt
    62221 cataagaaaa tgagtacaga taaaaggagc aaatcaatgc caaattacaa tattccattt
    62281 tcaccgcctg atatcacaga agcagaaatt gctgaagtag cggataccct gcgttctggt
    62341 tggatcacaa caggtcctaa aacaaaagaa ctggagcgcc gcttgtctct ttacacccag
    62401 acacctaaga ctgtttgtct caactctgcg acagctgctc tggagttgat tttacgcgtt
    62461 ttggaagtgg gacctggtga tgaagtcatc gttccagcca tgacctatac ggcttcatgt
    62521 agtgtcatta cgcacgtggg agcaacccct gtcatggtgg atatccaagc agatacgttt
    62581 gagatggact atgacctgct tgagcaagct atcactgaga aaactaaggt gattatccca
    62641 gtagagctcg cagggattgt ttgcgattat gaccgtttgt tccaagtcgt ggagaaaaaa
    62701 cgtgacttct ttaccgcttc aagcaagtgg caaaaggcct ttaaccgtat tgtcattgtc
    62761 tctgatagtg cccacgcttt gggatctact tataaaggac aaccttctgg ttctatcgct
    62821 gactttactt ccttctcatt ccatgccgtt aagaacttta caacggcaga aggtggaagt
    62881 gcgacttgga aagccaatcc agtgattgat gacgaagaga tgtacaagga attccaaatc
    62941 ctttcccttc acgggcaaac taaggatgct cttgccaaga tgcaactggg gtcatgggaa
    63001 tacgatatcg ttacaccagc ctataagtgc aacatgaccg atatcatggc ttcacttggt
    63061 ttggtacaat tggaccgcta tccaagtttg ttgcaacgcc gtaaggacat tgtggaccgc
    63121 tatgatagtg gttttgcagg ttctcgcatc catcctttgg cacacaagac tgaaactgtc
    63181 gaatcttcac gccacctcta catcacccgt gtagaaggag caagcctaga agaacgcaac
    63241 ctcatcatcc aagaattggc taaagcagga attgcaagta atgttcacta caaaccgctt
    63301 cctctcttga cagcctataa gaatcttgga tttgatatga cgaactatcc taaggcctat
    63361 gccttcttta agaatgaaat taccctccct cttcatacta aattaagcga tgaagaagta
    63421 gactatatca ttgagacttt caaaacagtt tctgaaaaag tgctaacttt atcaaaaaaa
    63481 tgacaaacta cagtcaagcg aaagtgatcc tgtccctaaa aagtctaatt gagtgtaaaa
    63541 actgttgttt tcaattgata atagtttaca cctgtagttg aggcctcttt ctcctcagag
    63601 agagaatttt tataggattt tcctttcttg tgggagtccc gtggtttgaa ataagatgtg
    63661 agcaatttgg tgtagcattt agaatcctta ctagacatca tttagaaaat ctagtgtctt
    63721 gttctagttt tcaattcacc ctattttttg aaagacgtga gtttccatga gtgagattgt
    63781 ggaaactcgc gtcttttttt gttttcagaa tattgttcaa agttttgtgc ctgtctttca
    63841 tgttctagtc attcttttgc atgatagaat tta
//
LOCUS       DAVHEX010000005        56443 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVHEX010000005 DAVHEX010000000
VERSION     DAVHEX010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 56443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56443
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.005"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            174..512
                     /locus_tag="VVL45_000311"
     CDS             174..512
                     /locus_tag="VVL45_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEU4186551.1"
                     /translation="MTLTIHVYYKGDGDSAIKFAREMLDSGLVKEIRNQKGNLKYEYF
                     LPIEKEGTILLIDQWINQETLDKHYQSKTMQKILDLRKKYHLQMQVERYIEDDSGMPE
                     SDKSFIDTGN"
     gene            702..2138
                     /locus_tag="VVL45_000312"
     CDS             702..2138
                     /locus_tag="VVL45_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEU4186552.1"
                     /translation="MTTFKDGFLWGGAVAAHQLEGGWQEGGKGISVADVMTAGRHGVA
                     REITLGVLEGKYYPNHEAIDFYHRYKEDIALFAEMGFKCFRTSIAWTRIFPKGDELEP
                     NEEGLQFYDNLFDECLKNGIEPVITLSHFEMPYHLVTEYGGWKNRKLIDFFARFAEVV
                     FKRYKDKVKYWMTFNEINNQANYQEDFAPFTNSGIVYEEGDNREAIMYQAAHYELVAS
                     ARAVKIGHEINPDFQIGCMIAMCPIYPVTCNPKDILMAMKAMQKRYYFADVHVLGKYP
                     EHIFKYWERKGISVDFTAQDKEDLLGGTVDYIGFSYYMSFAIDSHRENNPYFDYLETE
                     DLVKNNYVKASEWEWQIDPEGLRYALNWFTDHYHLPLFIVENGFGAIDQVAADGMVHD
                     DYRIEYLGAHIREMKKAVVEDGVDLMGYTPWGCIDLVSAGTGEMRKRYGFIYVDKDDN
                     GKGSYNRSPKKSFGWYKEVISSNGESVE"
     gene            2190..2429
                     /locus_tag="VVL45_000313"
     CDS             2190..2429
                     /locus_tag="VVL45_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family sugar transporter"
                     /protein_id="HEU4186553.1"
                     /translation="MIGSIAAILTTFAFLPQVFRVVKTKDTGSIALGMYVMQVIGIAL
                     WLAHGIRIGDLPLILANSVSFLLSGIILFYKLKYK"
     gene            2559..2873
                     /locus_tag="VVL45_000314"
     CDS             2559..2873
                     /locus_tag="VVL45_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEU4186554.1"
                     /translation="MAKALIICAAGMSSSLMAQKTTEFLKGKGKDIVVDAISATEGGK
                     AIAAAAFDLYLVSPQTKMYFKQFEEAGSKAGKPVVQIPPQAYIPIPMGIEKMANLILE
                     NI"
     gene            2991..4970
                     /locus_tag="VVL45_000315"
     CDS             2991..4970
                     /locus_tag="VVL45_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEU4186555.1"
                     /translation="MNVLNIKEKDILQFLIKNKERFVTSKELAEYLSCSDRTVRNVLK
                     LIEKTMIIQGVRLISKQGQGYQIFFENQGAYQEFRQTYELEEDYTKTAVSKGDDRLVF
                     ILNKLLFEQVPVLFDDLADELYVSRSTLSHDFRKIRVMLSEYNLSIESRANKGVYVSG
                     EERDKRRFIINYFLENQFFKTIHCYVKFNFFDQTVPLEEFARIVLDECQEANLKLSDF
                     VLQNLVVHIALSVIRLKSGFEIKNIDCQMTDDAIERKVAQRILSKVREVTNQEFPVQE
                     IDYITLHLLAKSQQCQKNQKNISEEVLKKSLFKTFQNLGLDDMYNFSSDFQLIEGLIT
                     HLMTLQVRLESRITLNNPLVDEIKQNYSDIFFMTREILANMDKFLEWTISDDEVAYVS
                     LHFLAAMERSKESTKFNILAICATGFGAAQMLRNRLETEFGKRVEVVDVIGYYELNQE
                     KLKGIDFIVSAVDLSNLYFQIPVFKVSVFLKSDEMEMIRKAMDQMQVSSYVQSSKINK
                     FENNGFRQYFSKENFLICTESDKVNLLEKMVEGLSVGESNEFEQSLLYGIKQREELSS
                     VVFSEKIAVPHPIQPFGTEGKVSVAICKDSLLWDNQSSHVQLVFLLSPSIYGNEGLAT
                     VTKKIVSLTENDELQNQLISCNNFEDFINIFEKIK"
     gene            4980..5294
                     /locus_tag="VVL45_000316"
     CDS             4980..5294
                     /locus_tag="VVL45_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEU4186556.1"
                     /translation="MNQEELQVAAFEIILHSGNARSEIHEAFAKMREGSFDDAESKLN
                     QSNEIILEAHHAQTKLLQEYASGVEIKIEIIMVHAQDHLMTTMTLLEVAKEMLALYKK
                     VN"
     gene            5325..5831
                     /locus_tag="VVL45_000317"
     CDS             5325..5831
                     /locus_tag="VVL45_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4186557.1"
                     /translation="MDKKERQKIEQQRREMALTNTFFNRYLLLRYSIALFFFGNIYWL
                     LNQFINPSPIIIFPIMLIVFSILATIEQFKLYGNRKEKLGITLMFVRIQMLISIGLLV
                     LTWTSWFKNLFPIFENNQVARLFVFVVLLLGLVLSLLDIRRIKKIYKRTDKAYQQFVQ
                     LEKNSLSL"
     gene            5911..7257
                     /gene="celB"
                     /locus_tag="VVL45_000318"
     CDS             5911..7257
                     /gene="celB"
                     /locus_tag="VVL45_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEU4186558.1"
                     /translation="MDQQNGLFGFLENHVMGPMGKLAQFKVVRAITAAGMAAVPFTIV
                     GSMFLVFSILPQAFSFWPIVADIFSASFDKFTSLYMVANYATMGSLSLYFVLSLAYEL
                     TKIYAEEEELNMNPLNGALLALMAFVMTVPQIIFDGGMMKTVTSLKEGAVIADGWAMG
                     NVVARFGTTGIFTAIIMAIVTVLIYRMCVKHNWVIKMPEAVPEGVSRGFTALVPGFVV
                     AFVVIFINGLLVAMGTDIFKVIAIPFGFVSNLTNSWIGLMIIYLLTQLLWIVGIHGAN
                     IVFAFVSPIALANMAENAAGGHFAVAGEFSNMFVIAGGSGATLGLCLYIAFASKSEQL
                     KAIGRASVVPALFNINEPLIFGLPIIYNPALAIPFILAPMVTATIYYVANSLNFIKPI
                     IAQVPWPTPVGIGAFLGTADLRAVLVALVCAFAAFLVYLPFIRVYDQKLVKEEQGI"
     gene            7636..7812
                     /locus_tag="VVL45_000319"
     CDS             7636..7812
                     /locus_tag="VVL45_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4186559.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            8213..10426
                     /locus_tag="VVL45_000320"
     CDS             8213..10426
                     /locus_tag="VVL45_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEU4186560.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSQTGSFEDRTTQLIYNRDFGQ
                     ISLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVQTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(10648..11124)
                     /locus_tag="VVL45_000321"
     CDS             complement(10648..11124)
                     /locus_tag="VVL45_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEU4186561.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLQDQKSGPLGKRVEWNFAKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            11347..14547
                     /locus_tag="VVL45_000322"
     CDS             11347..14547
                     /locus_tag="VVL45_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HEU4186562.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTIGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSAWVDQKNSADASTRVIEAKDGA
                     ITISSHEKLRAALHRMVPIEAKKKYKLRFKIKTDNKIGIAKVRIIEESGKDKRLWNSA
                     TTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVADQLSEDSQTD
                     KQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKEGTTNVIVSKDGKEV
                     KKIPLKILASVKDAYTDRLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSIS
                     SQADRTYLWEKFSNYKTSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSMEW
                     MHKHVYNSEKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDPE
                     HFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGFY
                     QDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKNPIDKDKMQTMYHWIDKSFAPL
                     LVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQS
                     DSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRPSYLSAFNKMDKTAMYNAEKGFGFG
                     LSLFSSRTLNYEHMNKENKRGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTET
                     DAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFLG
                     SNIQNTSTDTAATTIDQRKLESGNPYKVYVNDKEASLTEQEKDYPETQSVFLESFDSK
                     KNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNRDSYGYML
                     IPNVDRATFNQMIKELESSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVLK
                     RGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKNH
                     SDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            14931..15316
                     /gene="tnpA"
                     /locus_tag="VVL45_000323"
                     /pseudo
     CDS             14931..15316
                     /gene="tnpA"
                     /locus_tag="VVL45_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(15695..16324)
                     /locus_tag="VVL45_000324"
     CDS             complement(15695..16324)
                     /locus_tag="VVL45_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEU4186563.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYITLR"
     gene            complement(16334..17335)
                     /locus_tag="VVL45_000325"
     CDS             complement(16334..17335)
                     /locus_tag="VVL45_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEU4186564.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITVAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEELFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSSTSIENLLANAQDIIR"
     gene            complement(17366..18007)
                     /locus_tag="VVL45_000326"
     CDS             complement(17366..18007)
                     /locus_tag="VVL45_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEU4186565.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKGYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKIIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            complement(18026..18841)
                     /locus_tag="VVL45_000327"
     CDS             complement(18026..18841)
                     /locus_tag="VVL45_000327"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEU4186566.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIQAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNTEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
     gene            19112..19546
                     /locus_tag="VVL45_000328"
     CDS             19112..19546
                     /locus_tag="VVL45_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU4186567.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
     gene            19558..20748
                     /locus_tag="VVL45_000329"
     CDS             19558..20748
                     /locus_tag="VVL45_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEU4186568.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDYVSYWDLIFNDGSDQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            20759..21250
                     /locus_tag="VVL45_000330"
     CDS             20759..21250
                     /locus_tag="VVL45_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEU4186569.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVTIKE
                     INIGNIHNAPGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            21265..22044
                     /locus_tag="VVL45_000331"
     CDS             21265..22044
                     /locus_tag="VVL45_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU4186570.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            22031..22849
                     /locus_tag="VVL45_000332"
     CDS             22031..22849
                     /locus_tag="VVL45_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU4186571.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPAKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            22849..23142
                     /gene="yajC"
                     /locus_tag="VVL45_000333"
     CDS             22849..23142
                     /gene="yajC"
                     /locus_tag="VVL45_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU4186572.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIQDDLVSLQIAKGVVIHVEKESVMGKTKELLFK"
     gene            23164..25065
                     /locus_tag="VVL45_000334"
     CDS             23164..25065
                     /locus_tag="VVL45_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEU4186573.1"
                     /translation="MKEERRQFFERIDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWAYMLNRQEYLLQFMIGYLVEGDKDYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSYYRAKYTLSNWGILQTIPMLAIYHFFSDKMNLEEAYHFASKELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYQELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEMLSLSAVVLNQEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     RRAWQPKSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGIHY
                     IEGAYWSAEPDLPYLHKRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDIGVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            25125..26126
                     /locus_tag="VVL45_000335"
     CDS             25125..26126
                     /locus_tag="VVL45_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU4186574.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(26236..26571)
                     /locus_tag="VVL45_000336"
     CDS             complement(26236..26571)
                     /locus_tag="VVL45_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4186575.1"
                     /translation="MKSLARLLIIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(26582..26824)
                     /locus_tag="VVL45_000337"
     CDS             complement(26582..26824)
                     /locus_tag="VVL45_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4186576.1"
                     /translation="MKKEKKQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            complement(26835..27377)
                     /locus_tag="VVL45_000338"
     CDS             complement(26835..27377)
                     /locus_tag="VVL45_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HEU4186577.1"
                     /translation="MKKETFTEKLIKRTYGISGPLDEYKRREADRIGNQVFIILFYLM
                     IFGNLIPLLLAYKYPQEVALIYPPLILVIALIASGYVTYQMKKTGITVIEPDMLNEKE
                     SKQLHYPGLKAGLFFGLWMFFITPLLSILIDEGQDYFHSLLTIRNGVSSILGSIFFGA
                     SIQFLISRRIAKTKKNQDED"
     gene            complement(27393..27587)
                     /locus_tag="VVL45_000339"
     CDS             complement(27393..27587)
                     /locus_tag="VVL45_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU4186578.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            27753..28703
                     /gene="rsmH"
                     /locus_tag="VVL45_000340"
     CDS             27753..28703
                     /gene="rsmH"
                     /locus_tag="VVL45_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEU4186579.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNHYDYHDLARIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK"
     gene            28715..29032
                     /gene="ftsL"
                     /locus_tag="VVL45_000341"
     CDS             28715..29032
                     /gene="ftsL"
                     /locus_tag="VVL45_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEU4186580.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            29036..31288
                     /gene="pbpX"
                     /locus_tag="VVL45_000342"
     CDS             29036..31288
                     /gene="pbpX"
                     /locus_tag="VVL45_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HEU4186581.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAVFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELETAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQISQQTVDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFNADTKEGITKDFVWRDILYQSNYE
                     PGSAMKVMTLAASIDNNTFPGGEYFNSSELKIADVTIRDWDVNEGLTGGGMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGLT
                     NYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KSLEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKAEEVPDMYGWTKATAETFAKWLNIELEFEGSGTTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            31290..32270
                     /locus_tag="VVL45_000343"
     CDS             31290..32270
                     /locus_tag="VVL45_000343"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEU4186582.1"
                     /translation="MFISISAGIVTFLLTVVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDILSVFGYPVHLGFFYIFFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLSSLLTLAILYLM"
     gene            32482..32622
                     /locus_tag="VVL45_000344"
     CDS             32482..32622
                     /locus_tag="VVL45_000344"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4186583.1"
                     /translation="MADRTDEVSSKHGFEVVDKEKLMWFEEVFEECKKYWSVKVKTLD
                     LF"
     gene            32878..34983
                     /locus_tag="VVL45_000345"
     CDS             32878..34983
                     /locus_tag="VVL45_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU4186584.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRATGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELEKKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYIIEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGVLVIREKV"
     gene            complement(35279..35761)
                     /locus_tag="VVL45_000346"
     CDS             complement(35279..35761)
                     /locus_tag="VVL45_000346"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEU4186585.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
     gene            complement(35856..37340)
                     /locus_tag="VVL45_000347"
     CDS             complement(35856..37340)
                     /locus_tag="VVL45_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEU4186586.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFRNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIITGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            37493..39100
                     /locus_tag="VVL45_000348"
     CDS             37493..39100
                     /locus_tag="VVL45_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEU4186587.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGVGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIAAIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENTDSPERDYYIWRDEPNDLNSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPAIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTDQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLIRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVELL"
     gene            39265..39432
                     /locus_tag="VVL45_000349"
     CDS             39265..39432
                     /locus_tag="VVL45_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HEU4186588.1"
                     /translation="MKKRILSAGLTFASALLLAACDQSGSDTKTYSSTFSGNPTTFNY
                     LLDYYADNIVN"
     gene            complement(39425..39946)
                     /locus_tag="VVL45_000350"
                     /pseudo
     CDS             complement(39425..39946)
                     /locus_tag="VVL45_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            40169..41614
                     /locus_tag="VVL45_000351"
     CDS             40169..41614
                     /locus_tag="VVL45_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEU4186589.1"
                     /translation="MSRRFKKSRSQKVKRSVNIVLLTIYLLLVYFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLVFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSEIENVTQLMSVTAPTGTDNENIQKLLADIKSSQNTDLT
                     VNQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEHPDYASKIKKIYTKGFTKKVEAP
                     KTSKDQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKLIDLLGGVDVYNDQ
                     DFTSLHGKFHFPVGNVHLDSEQALGFVRERYSLADGDHDRGRNQQKVIAAILQKLTSS
                     EALKNYSRIIDSLQDSIQTNMPLETMINLVNAQLESGGTYKVNSQDLKGMGRTDLPSY
                     AMPDSNLYMMEINDSSLASVKTAIQDMLEGR"
     gene            41616..42347
                     /gene="cps4B"
                     /locus_tag="VVL45_000352"
     CDS             41616..42347
                     /gene="cps4B"
                     /locus_tag="VVL45_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4B"
                     /protein_id="HEU4186590.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLAESYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVASDLVIAYGAEIYYTSDVLDKLEKNRIPTLNNS
                     RYALIEFSMNTPYRDIHSALSKILMLGITPVIAHIERYDALENNEKRVRELINMGCYT
                     QVNSSHVLKSKLFGERYKFMKKRAQYFLERDLVHIIASDMHNVDSRPPHMAEAYDLVS
                     QKYGEAKAQELFIDNPRKIVMDQLI"
     gene            42356..43048
                     /gene="cpsC"
                     /locus_tag="VVL45_000353"
     CDS             42356..43048
                     /gene="cpsC"
                     /locus_tag="VVL45_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HEU4186591.1"
                     /translation="MKEQNTIEIDVFQLLKTLWKHKLIILLVALVTGAGAFAYSTFIV
                     KPEYTSTTRIYVVNRNQGDKPGLTNQDLQAGTYLVKDYREIILSQDVLEKVATNLKLD
                     IPAKTLTSKVQVTVPADTRIVSISVKDKQPEEASRIANSLREVAAEKIIAVTRVSDVT
                     TLEEARPAMTPSSPNVRRNTLVGFLGGAAVTVITVLLIELLDTRVKRPEDVEDVLQMP
                     LLGVVPDFDKMK"
     gene            43058..43747
                     /locus_tag="VVL45_000354"
     CDS             43058..43747
                     /locus_tag="VVL45_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HEU4186592.1"
                     /translation="MPTLEISQAKLELVKKAEEYYNALCTNLQLSGDDLKVFSITSVK
                     AGEGKTTTSTNIAWAFARAGYKTLLIDADMRNSVMSGVFKSRERITGLTEFLSGTTDL
                     SQGLCDTNIENLFVIQAGSVSPNPTALLQSKNFSTMLETLRKYFDYIVVDTPPVGVVI
                     DAAIIMQKCDASILVTKAGETKRRDLQKAKEQLEQTGKSCLGVVLNKLDTSVEKYGSY
                     GSYGNYRKQKK"
     gene            43762..45123
                     /locus_tag="VVL45_000355"
     CDS             43762..45123
                     /locus_tag="VVL45_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU4186593.1"
                     /translation="MSEKLAKPSVAIVQSFLVILLTYLLSAVRETEIVSTTAIVLYIL
                     HYFVFYISDYGRNFFKRGYLIELVQTLKYILFFALAISISNFFLEDRFSISRRGMIYF
                     LLLHVFLVYVLNPFIKWYWKRAYPNFKGSKKILLLTATSRVEKVLDRLIESNDVVGEL
                     VAVSVLDKPDFQHDDLKVVAEGEIVNFATHEVVDEVFINLPSEKYNIGELVSQFETMG
                     IDVTVNLNAFDWARNKQICEMAGLNVVTFSTTFYKTSHVIAKRIIDIVGSLVGLILCG
                     LVSIVLVPLIRKDGGSAFFAQTRIGKNGRHFTFYKFRSMCVDAEEKKRELMEQNTMQG
                     GMFKVDDDPRITKIGHFIRKTSLDELPQFYNVLKGDMSLVGTRPPTVDEYEHYTPEQK
                     RRLSFKPGITGLWQVSGRSEIKNFDEVVKLDVAYIDGWTIWKDIEILLKTVKVVLMKD
                     GAK"
     gene            45129..45872
                     /locus_tag="VVL45_000356"
     CDS             45129..45872
                     /locus_tag="VVL45_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEU4186594.1"
                     /translation="MNERIQILGITIDPLTMKETVDAVEQYVLKKHPLHLMGVNADKI
                     NQCYEDEKIKKIVNESGIINADGASVVLASKFLGTPVPERVAGIDLMKHLLELSNEKG
                     YSVYFFGAKEDVLQDMLKIFEERYPALNVVGYRNGYFSPEDEKHIQEDIKEKKPDFVF
                     VGITSPKKEYIIQSFMDNGINAVFMGVGGSFDVLSGHIKRAPLWMQNAHLEWLFRVAN
                     EPKRLFKRYFVGNVSFIRKVLRAKRGYKL"
     gene            45869..46693
                     /locus_tag="VVL45_000357"
     CDS             45869..46693
                     /locus_tag="VVL45_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HEU4186595.1"
                     /translation="MNQTEIVKLIQKVELDAIKEFQKICKENNIDFFLRGGSVLGAVK
                     YDGFIPWDDDMDIAVPREGYDKLPGIFKDRIIAGKYQVLAYQYCDTLHCYFPRLFLLE
                     DERKRLGLPRNTNLGLHLIDIIPLDGAPNHSFLRKLYFGKVYWYRFLASLGTTYVGDH
                     VDMHSAKQKLIIGFFKKLGFAKLFPQNYVYRRLDNLYKKYDWKKQKYAGTINASLFAK
                     EVMPVEIWGEGVEKPFEDAFFKVPTEYDRYLKRLYGENYLHEEPSDDEKKSHLGGQ"
     gene            46695..47573
                     /locus_tag="VVL45_000358"
     CDS             46695..47573
                     /locus_tag="VVL45_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU4186596.1"
                     /translation="MFCYIILHYKVLEETISCVKSIKEGNSNTKQIVIIDNFSNNGTG
                     EKLQELYESDSEIDVLINHENAGFARGNNVAYQFAKEKYKPDFMVIMNNDIEIETEEF
                     EKIVTDIYRKEKFHLLGPDIFSTTYQLHQNPKRLTHYTYEEVKALNEKFKKGSQVSLA
                     LKIKCWLKSSKVLRTAIYQNRRKKKSVDYRKQVENPILHGSFIVYSRDFIEKEEYAFN
                     PNTFFYYETEILDYEAELKGYKRIYTPKIKVLHHQNVATNQVYTNLVEKTLFSNKCNF
                     ESTSYFLKLMKENEGV"
     gene            47574..48908
                     /locus_tag="VVL45_000359"
     CDS             47574..48908
                     /locus_tag="VVL45_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HEU4186597.1"
                     /translation="MTYLFLLCLTLFLLTFFYFFAFNQDLIAPPVVMSVMFLISSVFA
                     LINVQNWNIEYSGLAYLLIISGIIVFSMPLLALNSPSLNTKIKVTDRLIDIQFWKIAL
                     TIIVDLVILYLYRREIHNLALSHGYTGSNFQWFFRNATSYEGELTVRTSIRVLIRIID
                     VSAYIFGYTFINNFFIYSHKRSKDLLLLVPFLIFISKTLLSGGRLDIIKILIAYVVMA
                     YIQQKRKVGWDKVISHKYMRLGFVGLIAGIPTFYYSLFLSGRSTTRTVFESISTYLGG
                     SIQHFNQYIQNPIGVAEVFGDESFVAIMNILGNLGFVNYNSTVHLEFRQLGITMGNVY
                     TFFRRPWHDFGLVGMYIFSFVVGVFFAIFYLKLRKSRAGFKLDIHTIIYSYFFYWIFL
                     SSIEQYSFTTISLFTLVFIVLVYLMAFFYWNLDFHRGKLVIKLSDTSIKSEN"
     gene            48936..50360
                     /locus_tag="VVL45_000360"
     CDS             48936..50360
                     /locus_tag="VVL45_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4186598.1"
                     /translation="MNSKIKNILTNFSYVISSNLLTVLTSSLVVLIFPKLMGVTEYSY
                     WQLYIFYLTYIGFFHLGWIDGIYLKYGGLEYNNLDRKQFYSQMILFSSFLMIISLLLF
                     GLNVLAVTDSNARYIYNMTIISMIITNLRTLYVYVLQMTNRLKDSSIILLSDRVLYVL
                     LLFLFIVFGWHEYKVMILADILGRSFSLILSFWICKDIVFQPLSKFIFNIKESFDNIR
                     VGINLMLSNIASSLIIGIVRLGIQWNWNIETFGKVSLTLSVSNLLMTFINAIGLVVFP
                     LLRRTKAENLPKIYSNLRNVLMLIMFAILLIYYPLKIVLDLWLPAYQDALIFMTLIFP
                     MSVYEGKMALVINTYLKALRMERDILRVNALVMLISMGVTLVTTYLLNSLELTVVSIV
                     VLLALRSIIAELILSKKLDVSVKKDIVLEFLLTLVFISSSWYLPIGLAVIVYTIAYGL
                     YLYLKHEDIKTYLAYFKASKKTSN"
     gene            50425..51513
                     /gene="wecB"
                     /locus_tag="VVL45_000361"
     CDS             50425..51513
                     /gene="wecB"
                     /locus_tag="VVL45_000361"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEU4186599.1"
                     /translation="MKKIMLVFGTRPEAIKMCPLVNELKKHEDMETIVCVTGQHKEMV
                     SPVLDLFGVVPDYDLEIMKANQTLFSITTSILEKIKPVLEKEQPDIVLVHGDTTTTYA
                     AALAAFYLGIKVGHVEAGLRTYNLQSPFPEEFNRQSTSIIATYHFAPTELAKENLLKE
                     GRENVYVTGNTVIDALTTTVQEDYTHTHLDLNANNRLILLTAHRRENLGEPMRHMFRA
                     VKRVLNEYEDVKVIYPIHKNPLVRETAAEIFGDTERIQIIEPLDVLDFHNFMNQSYMI
                     LTDSGGVQEEAPSLGKPVLVMRDTTERPEGVAAGTLKLVGTDEETIYQNFKLLLDDSG
                     EYKKMSQASNPYGNGDASQQIVQILRGI"
     gene            51676..52545
                     /gene="rfbA"
                     /locus_tag="VVL45_000362"
     CDS             51676..52545
                     /gene="rfbA"
                     /locus_tag="VVL45_000362"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEU4186600.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLA
                     GIKDILIISTPQDLPRFKDLLLDGSEFGIKLSYAEQPSPDGLAQAFLIGEEFIGDDSV
                     ALILGDNIYHGPGLSTMLQKAAKKEKGATVFGYQVKDPERFGVVEFDTDMNAISIEEK
                     PEYPRSNYAVTGLYFYDNDVVEIAKQIKPSARGELEITDVNKAYLNRGDLSVELMGRG
                     FAWLDTGTHESLLEASQYIETVQRMQNVQVANLEEISYRMGYISREDVLELAQPLKKN
                     EYGQYLLRLIGEA"
     gene            52546..53139
                     /locus_tag="VVL45_000363"
     CDS             52546..53139
                     /locus_tag="VVL45_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEU4186601.1"
                     /translation="MSDNFFGKTLVVRKIDAIPGLLEFDIPVHGDNRGWFKENFQKEK
                     MLPLGFPESFFAAGKLQNNVSFSRKNVLRGLHAEPWDKYISVADDGKVLGSWVDLREG
                     ETFGNVYQTEIDASKGIFVPRGVANGFQVLSDTVSYSYLVNDYWALELKPKYAFVNYA
                     DPALGIEWENLAEAEVSEADKHHPLLRDVKPLTKDEL"
     gene            53151..54200
                     /gene="rfbB"
                     /locus_tag="VVL45_000364"
     CDS             53151..54200
                     /gene="rfbB"
                     /locus_tag="VVL45_000364"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEU4186602.1"
                     /translation="MTEYKKIIVTGGAGFIGSNFVHYVYNNFTDVHVTVLDKLTYAGN
                     RANIEEILGDRVELVVGDIADAALVDKLAAEADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGTYTLLEAARKYDLRFHHVSTDEVYGDLPLREDLPGHGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLY
                     GEGKNVRDWIHTNDHSSGVWTILTKGQVGETYLIGADGEKNNKEVLELILKEMGQPAD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWKPEFTNFEAGLKATIKWYTDNQDWWKAEKE
                     AVEANYAKTQEIITV"
     gene            54266..55117
                     /gene="rfbD"
                     /locus_tag="VVL45_000365"
     CDS             54266..55117
                     /gene="rfbD"
                     /locus_tag="VVL45_000365"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEU4186603.1"
                     /translation="MILITGANGQLGTELRYLLDERNEEYVAVDVAEMDITNEEMVEK
                     VFEEVKPTLVYHCAAYTAVDAAEDEGKELDFAINVTGTENVAKASEKHGATLVYISTD
                     YVFDGKKPVGQEWEVDDRPDPQTEYGRTKRMGEELVEKHVSNFYIIRTAWVFGNYGKN
                     FVFTMQNLAKTHKTLTVVNDQYGRPTWTRTLAEFMTYLAENRKEFGYYHLSNDATEDT
                     TWYDFAVEILKDTDVEVKPVDSSQFPAKAKRPLNSTMSLAKAKATGFVIPTWQDALQE
                     FYKQEVR"
     gene            55180..55514
                     /locus_tag="VVL45_000366"
                     /pseudo
     CDS             55180..55514
                     /locus_tag="VVL45_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272501.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
     gene            55781..56366
                     /locus_tag="VVL45_000367"
                     /pseudo
     CDS             55781..56366
                     /locus_tag="VVL45_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
BASE COUNT        17654 a         9468 c        12152 g        17169 t
ORIGIN      
        1 tgctcttgtt tcaattgact ataaatcgct ataagaaaaa atcattttat tggtataaag
       61 aagtgattga aagtaatggc gaaacgttag ataattagtt cataaaaaac aagtttagat
      121 cctcatctaa acttgttttt ttaaaattga aggatgattt tggaggatat aagatgacat
      181 tgactattca tgtatattat aaaggtgatg gagatagtgc aataaagttt gcaagagaaa
      241 tgcttgatag tggccttgtt aaagaaatta gaaatcaaaa aggtaacttg aaatacgaat
      301 attttttacc aattgaaaaa gagggaacta ttctccttat tgatcagtgg ataaatcaag
      361 aaactttaga taaacattat caatcaaaga caatgcagaa aatcttagat ttaagaaaaa
      421 aatatcactt gcagatgcaa gttgaacgtt atatagaaga tgattctggg atgccagaaa
      481 gcgataagtc gtttatagac acagggaatt agaattattg tgagagtttt tgtagtttga
      541 aagtattgaa aatcgtaaag atatcattac gagaatacaa gaagatattg ttctatttcc
      601 attaaaaatt acttctttcc ttaacaataa ggaaaaagaa tgcgttttca tcttttggtt
      661 taaggtttat aataagagta tcaaagtcag gaggaaaaaa gatgactact tttaaagatg
      721 gatttttatg gggtggtgct gttgctgctc atcaacttga aggtggatgg caagaaggtg
      781 gcaagggaat tagtgttgct gatgttatga ctgctggtcg tcatggagta gctcgtgaaa
      841 ttactttggg agttttagag ggtaaatatt atccaaatca tgaggcgata gatttttatc
      901 accgttataa agaagatata gcactttttg ctgaaatggg attcaagtgc ttccgtacct
      961 ctattgcatg gacacgtatc tttccaaaag gtgatgagtt agagccgaat gaagaaggat
     1021 tacagtttta tgataatctt tttgatgaat gcttaaagaa tggtattgaa cctgtcatca
     1081 ctctatctca ttttgaaatg ccttatcact tagtgaccga atatggtggt tggaaaaata
     1141 ggaaattgat tgatttcttt gctcgttttg cagaagtcgt atttaaacgt tacaaagata
     1201 aggttaaata ttggatgact ttcaatgaaa tcaataatca agcgaattat caggaagatt
     1261 ttgcaccatt tactaactca ggtattgtat atgaggaagg tgataataga gaagcaatta
     1321 tgtatcaagc agcacattac gaattagttg cttctgcacg agctgtaaaa attggtcatg
     1381 agattaatcc agattttcaa ataggttgta tgattgcgat gtgtccaatt tatccagtta
     1441 cttgcaatcc taaggatatc ttaatggcaa tgaaagctat gcagaagcgt tattattttg
     1501 ctgatgtgca tgttttaggt aaatatcctg agcatatttt caagtattgg gaacgaaaag
     1561 gtatttcagt tgattttact gcccaggata aagaagattt acttggtggg actgtagatt
     1621 acattggttt cagttactat atgtcctttg ctatcgactc tcatcgtgaa aataatcctt
     1681 attttgatta tcttgaaaca gaagatttag tgaaaaataa ttatgttaag gcttctgaat
     1741 gggagtggca aattgatcca gaaggtttgc gttatgcgtt aaattggttt acagaccact
     1801 atcacttacc actctttatt gttgaaaatg gttttggagc tatagatcaa gttgcagcag
     1861 atggtatggt acatgatgat tatagaattg aatatctagg tgcccatatt cgtgaaatga
     1921 aaaaggctgt agttgaagat ggtgttgatt taatgggtta tactccatgg ggatgtattg
     1981 atttggtttc agctggtacc ggtgaaatgc ggaaacgtta tggctttatt tatgtagata
     2041 aagatgataa tgggaaggga agttataatc gttccccgaa aaaatctttt ggctggtata
     2101 aggaagttat ttcatctaac ggtgaatcag tagaatagta taaacaaact gagattattc
     2161 tcagtttgtt tagcatattg aggtgacata tgattggaag tattgcagca attttaacaa
     2221 catttgcatt tttaccacaa gtttttcgtg tagttaagac aaaggacaca ggatctattg
     2281 ctctaggtat gtatgttatg caagttattg gtattgcatt gtggcttgct catggtattc
     2341 gtataggaga cttgcctttg attttagcaa atagtgtttc atttctactc tcaggaataa
     2401 ttttatttta taaattgaag tataaataat ttttttccat atcatttagg aaaatgaggg
     2461 aattgtcatt ggaaacgctt tattttataa taaaaataga taacaaataa gccaacttag
     2521 cacagctttg attggattta agaaaaggag aaaatattat ggcaaaagct ttaattattt
     2581 gtgcagcagg aatgtcttca tcacttatgg cacagaaaac aacagagttt ctaaaaggaa
     2641 aagggaaaga tattgtagtt gatgctatta gtgctactga aggagggaaa gctatcgcag
     2701 cagcagcttt tgatttgtat cttgtcagtc ctcaaacaaa aatgtacttt aagcaatttg
     2761 aagaggctgg ttctaaagca ggaaaaccag ttgtacaaat acctcctcaa gcctatattc
     2821 ctattccaat gggaattgaa aaaatggcta atttgattct tgagaatatt tgatgaacac
     2881 atgaaatgtc aataaatatt taggttaaga taaattgttt tgagtttgat ataaactgat
     2941 ttaagtgtat aatatgcttc aatatatgaa aacatacgtt ttaggaggga atgaatgtgt
     3001 taaacattaa agagaaagat atccttcaat ttttaataaa aaataaggaa cgttttgtta
     3061 caagcaaaga actggcagaa tatttatcct gttcagatag aactgtccgt aatgtactga
     3121 aattaattga gaagacaatg attattcaag gagtccggtt gatttcaaaa caaggtcaag
     3181 ggtatcaaat attttttgag aatcaaggcg cttaccagga gtttagacaa acttatgaac
     3241 tggaagaaga ctatacgaaa actgcggttt caaaaggtga tgatcgttta gtatttatct
     3301 taaataaatt attatttgag caagttccag ttctttttga tgatttagct gatgaacttt
     3361 acgttagccg ttcaacatta tctcatgatt tcagaaagat acgagtgatg ttatcagagt
     3421 ataatttgtc cattgagagt agggcgaata aaggagttta tgtctcaggc gaggaacgtg
     3481 ataaacgacg ttttatcata aattatttcc tagagaatca attttttaaa acaatacatt
     3541 gctatgtaaa atttaacttt tttgatcaaa ctgttccatt agaagaattt gctagaattg
     3601 ttttggatga atgccaagag gccaacctaa aactatctga ttttgtacta cagaatttag
     3661 tagttcacat agcattgtct gtgattcgtt taaaatccgg atttgaaata aaaaatatag
     3721 actgtcagat gactgatgat gcaatagaga gaaaggttgc ccaaagaatt ctttctaaag
     3781 taagagaagt aacaaatcag gaatttccag ttcaggaaat agactacatt actttgcatt
     3841 tgttagctaa gagtcaacaa tgtcaaaaaa atcagaagaa tatttctgaa gaagttttaa
     3901 aaaaatcttt atttaaaaca tttcagaatt taggattaga tgatatgtat aacttttctt
     3961 cagatttcca actaattgaa ggattgataa ctcatctaat gacactacaa gtacgtttag
     4021 aaagccggat taccttaaat aaccctttgg tagatgaaat taaacagaat tatagtgata
     4081 tattctttat gactagagaa attcttgcaa atatggacaa gttcttggag tggacaatat
     4141 cagatgatga agtagcatat gtaagtcttc actttttagc tgctatggaa cgtagcaaag
     4201 agagtactaa gtttaatatt cttgctattt gtgcaacagg atttggtgca gcccaaatgt
     4261 taagaaatcg tttggaaacg gagtttggga agcgagtaga agttgttgat gtaataggct
     4321 actatgaatt gaatcaagaa aaacttaaag gaatagattt tatagtgtca gctgttgatt
     4381 tatcaaatct atattttcaa atcccagttt ttaaagttag tgtgtttctt aaaagtgatg
     4441 agatggaaat gattagaaaa gctatggatc agatgcaagt ttctagttat gtacaatcat
     4501 caaagattaa caaatttgaa aacaacggct ttagacagta ttttagtaaa gaaaattttt
     4561 taatatgtac tgaatcagac aaggtaaatt tacttgaaaa gatggttgag ggtttatctg
     4621 ttggtgaatc aaatgagttt gaacaatctt tactttatgg aattaaacag cgcgaagaat
     4681 taagttcagt tgttttttct gaaaaaattg cagttccgca tcctattcag ccctttggta
     4741 cagagggaaa ggtttcggtt gcaatttgta aagattcgtt actctgggat aatcaaagtt
     4801 cacatgtcca gctagtattt cttctatctc catcaatata tgggaacgaa ggtttagcaa
     4861 ctgtgactaa aaagatagtc tctctgacag agaatgatga attgcaaaat cagctaatat
     4921 cttgcaataa ctttgaagat tttattaaca tatttgagaa gataaaatag gaggttcata
     4981 tgaatcaaga ggaactgcag gttgcagcat ttgaaattat cctgcattcg ggaaatgcaa
     5041 ggtcagaaat acatgaagca tttgcaaaaa tgagagaagg tagttttgat gatgcagaaa
     5101 gtaaattaaa tcaatcaaat gagataattc ttgaagcgca tcatgcacaa accaaactct
     5161 tgcaagaata cgcaagtgga gtggaaatta aaattgaaat tatcatggta catgctcaag
     5221 atcatttaat gaccactatg acattattag aagtagcaaa ggaaatgtta gctctttata
     5281 agaaagttaa ctaaatatgt gttgttatat aaggaggaaa tgatatggat aaaaaagaac
     5341 gacaaaaaat tgaacaacaa cgtcgagaga tggctctaac taatactttt tttaatcgat
     5401 atcttcttct acgttattca atagcactct tcttttttgg gaatatttat tggcttttga
     5461 atcagtttat taatccctcg cctattatta ttttccccat tatgctcata gtattttcta
     5521 ttttagcaac tattgaacaa tttaaacttt atggaaatag gaaagaaaag ttgggaataa
     5581 cactaatgtt tgttagaata caaatgctta tttcaatagg attacttgtt ttaacttgga
     5641 caagttggtt taagaatctt tttccgattt ttgaaaataa ccaagtagca cgtttatttg
     5701 tttttgtagt tcttctatta ggtttagttc tgagtttgtt agacattaga agaattaaaa
     5761 aaatttataa acgaacagat aaggcttatc aacaatttgt ccaattggaa aagaactcac
     5821 ttagtttata atcctgatct agtataattt gtatttgaaa ctagtcttta gaaagtaaaa
     5881 attattatgt tttattaagg aggtctattt atggatcaac aaaacgggtt gtttggtttt
     5941 cttgaaaacc atgttatggg accaatgggc aaacttgctc agtttaaagt agtacgtgct
     6001 atcacggctg caggtatggc tgctgtacca tttactattg taggatcaat gtttttggta
     6061 ttcagtattt tgccacaagc tttctcattt tggccaattg tggcagatat tttctctgct
     6121 tcatttgata aattcacatc actttacatg gttgcaaact atgcgactat gggttctcta
     6181 tctctttatt tcgttctatc acttgcatat gaattgacaa aaatttatgc agaggaagaa
     6241 gaactcaata tgaatcctct taatggtgcc ttgcttgcct tgatggcttt tgtcatgaca
     6301 gtaccgcaaa tcatttttga tggtggaatg atgaagactg tgacaagtct aaaagaaggt
     6361 gcagtaattg cagatggatg ggcaatggga aatgtagtcg cacgttttgg gacaacaggg
     6421 atttttaccg caatcattat ggcaattgtg actgttctta tttatcgtat gtgtgttaaa
     6481 cataattggg ttattaaaat gcctgaagct gttccagaag gagtttctcg tggatttacc
     6541 gctttggttc cgggatttgt tgttgcattt gttgttatct ttatcaacgg tcttcttgta
     6601 gcaatgggaa cagatatttt taaagtcatt gcaattccat ttggttttgt atccaatctg
     6661 actaattcgt ggattggttt aatgattatt tatctattga ctcaactact ttggattgta
     6721 ggtatccacg gtgcgaacat tgtttttgca tttgttagtc caattgctct tgctaacatg
     6781 gctgaaaatg ctgctggcgg gcacttcgct gttgcaggtg aattttctaa tatgtttgta
     6841 attgcaggtg gttctggtgc aactttagga ctatgtttat atattgcttt tgcctctaaa
     6901 tctgaacagc ttaaagcaat aggacgagca tctgtagttc cagccttatt taatattaat
     6961 gaaccattaa tttttggatt acctattatc tataatccag ccttggctat accatttatt
     7021 ttagcaccaa tggttactgc tactatttat tacgtagcga attctctaaa ctttattaag
     7081 ccaattatcg cacaggttcc atggccaact ccagtaggga ttggagcttt cttagggaca
     7141 gcagatcttc gagctgtatt agttgctcta gtatgtgcat ttgcagcatt cctagtctat
     7201 cttccattca tccgtgtata tgatcaaaaa ttggtgaaag aagagcaagg tatctaataa
     7261 gtaaaaaagt atactcgatg atagaatcca tatagtttaa ataatgagtg ttaatattag
     7321 cagtgaagag gtctttttaa gatattcttc tctgcttttt catatttttt cgctatgtag
     7381 ggtatgaagc aaaatagaaa gtagttcgaa tagttgataa acagggaact gctgttattt
     7441 aaaaaaacaa taaagtaaag tatgttatcg tagaatacga tacaatagta aaaaagcaaa
     7501 aagaatgcgt tttatatttg ggtttgaggc ttataaaata ggataagcgt gctgaaaact
     7561 tagatatgac aaacaaaata aaatttttaa aagctacaga gaaacatggt ataatgtatc
     7621 taaaggaggt agcatatgtc cttagatata gataaagaaa agatgacaat catgggaata
     7681 gcttttgaaa accgatccgt ttttaagagt gtttggtatg ctttaagtac aaatatgatt
     7741 gaaggatggc gacctacggt tagtgatgtt gaaaaattac gagatgaagc tcttgctctg
     7801 gggatgactt aatgaagcgc aactgttatg aatcttatga ctatgttgat ccggataatt
     7861 tatataccga tccgggttct tcagttctaa gaaataagca ggaaggaaaa aagcacgtga
     7921 gcttgaatat cgtatgattg ctagtaagag tttgaaatta tttatcaatc ctatcttagt
     7981 taatagttta aaagtagttt gtttcacaac tgcataagct tagaaaagtt gaaaagtgat
     8041 aactctttaa taaactcttg gtttgttaaa agatattttg tccactgcat tttttcaatt
     8101 gactattatt tgtcatagat ataacagata gaacattgca ttcagaataa tgaaaaacgc
     8161 atcaaaaatg gtgcgttctg tttttatgct accataaaag gaggaaaaca aattgaaaat
     8221 ttttaaggga gagttttatc gaatctctgt attaacagac aagctagtaa ggttagaata
     8281 ctctcaaact ggaagttttg aggatagaac gacacaactt atctataata gagattttgg
     8341 ccaaatttcg ttagattata tcgagacatc aaacgtacta gatattatga cggactattt
     8401 tcatctgcac tttaataaag gagaatttaa cgccgaaaat ttatttatag aattaaaagg
     8461 aaattttgcc gtatatggta gtcgctggta ttttggtgaa tctattgaaa cgttaaaagg
     8521 aacagctcgg actctggata aggcagatgg agcaatctcg ttagaagatg gaattattag
     8581 ccgaaatggt atagccttat tggatgattc tcaaggattt atttgggatg aacaatctgg
     8641 ttatattgag agagaaaatc aaattgacct gtatttcttt gcctatgggc atgattatag
     8701 aggagcaatc agagattttt accatttgac tggttcaaca cccttgttgc caagatatgc
     8761 tttaggcaat tggtggagta gatattggcc ttatacgtcg gatgaatact tggatttaat
     8821 agacagattt gaaacagaga aaattccatt atctatcggt gtgttagata tggattggca
     8881 tataactgac attccagctc gctttggaag tggctggaca ggatatagtt ggaatagaaa
     8941 cttaatacca aatccagaac agttattgca acaacttcat gatagaaagc taaaactctc
     9001 cttaaatgtc catcctgctg atgggatacg ggcttatgaa gaagcttatc ctcaaatcgc
     9061 aaaacggttg gggttaaatg tagaactaga agaacctgct atttttgatt tttttaatcc
     9121 ctcttttagg gaagcctact ttaaagatgt tcattatgaa ctagaaaagc agggagtaga
     9181 tttttggtgg attgactggc aacaagggac acaaggtatg ctagatccac tttggctttt
     9241 aaaccattat cactatcagg atagttgtaa aaatgcagaa ggtggtttga ttttatcaag
     9301 atatgcaggt cctggtagtc accgctaccc tgttggtttt tcaggggata ctattattag
     9361 ttggaattcc ttaagatttc aaccctattt tacagcgaca gcatctaata tcggttatag
     9421 ttggtggagt catgatatcg gtggacatat gttgggggat tatgacgaag agctacaaac
     9481 tagatggcta cagtttggtg tttttagtcc gattactcga ttacatagtt ctagaagtcc
     9541 ttttaatagt aaagaacctt ggtttttttc agaaacaaca tctaagatta tgaagaaata
     9601 ccttcgtttg agacatcaga tgattcccta tctatatacc atgaatgtac agacacatga
     9661 ggaaggtgcc ccattaatca gtccaatcta ttatttctat ccagagaatg atgagagcta
     9721 taatgttcca aaccaatact tttttggaac agaactgatg gtggctccca ttgtagaaaa
     9781 gatggatttg acattccaat ctgcaaaagt agatgtatgg ttccctgaag gtgaatggta
     9841 tgacttcttt tcagagaaaa aatacacagg tggtgtgaag ttaagtgttt atagggacat
     9901 ctcgactacg cctgtgtttg caaaaagtgg tgcaatcatt cccttggttg gttctgagat
     9961 aggtatgggt gttgatttac ctgaagttgt agattggtat gtattcccag gaaaacaaca
    10021 ttcttttgaa atgcttgaag atcaaaatgg tcaaagatat aaaacaagat tatcaatcga
    10081 ttgggaaatg ggaatggtag agttagcatt acaaggagat tctagtatcg ttccaagcaa
    10141 tagaaaacat agaattcatt ttaaaggaac gaatgtgtct ataattgaat tgccaaataa
    10201 gaatgataca gctaaatttg aatggaaaga taataaaagg acatctttaa atgacgaagt
    10261 ttttagacta ctaaagacag cttctcttcc atatgaatta aaagatagat tgttaaatca
    10321 attcatcaat gcgaaaaact ctcatgactt aatgaatatc ttgcatcatc aggataagga
    10381 attgagaggg cgtttgttgg aaatgatatt tactagccaa aactaattga aataattaga
    10441 aagtcatttg tttagtgtat atgaagattc tgtatggtat tcgttatcac tggtcacatt
    10501 gaaattgtga aataagcacc tacaacatag actttacaac acttcatgtt tcatatcatg
    10561 gggtgttttt ctatttaagt caaagcccat caaaaaatct cactattcat caggttaaag
    10621 gaaacctaat catagtgaga ttgtgaatta tagtagagtt tgtatcgcct cttcaatttg
    10681 ttttgggtct gtttttgaag aaaagcgttc aaagacttgc ccatctcgac cgatgagaaa
    10741 cttagcgaaa ttccattcga ctcgttttcc tagtgggccg gatttctggt cttgtaacca
    10801 gacatagaga gggtctgctt ccttaccgtt gaccttaatc ttggcaaaac gtgggaaggt
    10861 ggtttgaaaa tgtaggctac agaaggcgtt gatttcctct gcgctgccgg gtgcttgtcc
    10921 cataaactga ttgcaaggga aatccaatat ttcgaagccc tgatcttgat agcgttcata
    10981 gagttcttga agtccctggt actggggcgt taaaccacat ccagtagcag tgttgacaat
    11041 caagagaact ttgccacgat agctatccaa gggagttgct tggttgtttt ggttcaaaac
    11101 ggaaaaatca taaagtgaag tcatgagggc ctcttttctt tttctacatt gtaacagttt
    11161 ttactttttt cgtcgagtcg gatggaagat attgttgaag aataagcctt caatatgata
    11221 gaaaccgttt acaaataact ttaaaaatgg tatgatttaa ttaaaccggt ttacttagtt
    11281 gctataaatc tctaatttga gatttagttc ctattgattt tacaatatgt ttattggagt
    11341 gtatacatgc aaacaaaaac aaagaagctc attgtgagtt tgtcttcact tgttttatca
    11401 ggatttttat taaaccatta tatgacaatt ggagcggaag aaacgactac gaataccatt
    11461 cagcaaagcc agaaggaagt tcagtatcag caaagggata caaaaaattt agttgaaaat
    11521 ggtgattttg gtcagacgga ggacggaagc agtccgtgga caggaagcaa agctcagggg
    11581 tggtcagctt gggtagacca gaagaatagt gcagatgcct caactcgagt cattgaggct
    11641 aaggatgggg ctatcactat ctcaagccat gagaaattaa gggcagcgct tcaccgtatg
    11701 gttcctattg aagctaagaa aaagtataaa ctgcgtttca agattaaaac agataataaa
    11761 atcgggattg ccaaagttcg tatcattgag gaaagtggta aggacaagcg attgtggaat
    11821 tctgcaacga cgtcaggaac aaaggactgg cagaccattg aagcagacta tagcccgact
    11881 ttagatgttg ataaaatcaa gctggagtta ttctatgaaa caggaactgg gactgtttcc
    11941 tttaaggata ttgagctggt agaggtagca gaccagcttt ctgaggattc tcaaacagat
    12001 aaacagcttg aggaaaagat tgatttacca attggaaaaa aacatgtttt ttctcttgcg
    12061 gactatactt ataaggtaga aaatcctgac gttgcttcag tcaaaaatgg aattttagaa
    12121 cctcttaagg aagggacaac caatgtcatt gtcagtaaag atggcaagga agtgaaaaag
    12181 attcctttga agattctggc ctctgttaag gatgcataca cagaccgttt ggatgactgg
    12241 aatggcatca tcgctgggaa tcaatactat gattctaaaa atgaacagat ggccaaatta
    12301 aaccaggaat tggaaggaaa ggtagctgat agcctatcca gtatttcaag tcaggcggac
    12361 cgcacctatt tgtgggaaaa attttcaaat tataaaacgt ctgcaaatct gactgccact
    12421 tatcggaaat tggaggagat ggccaagcaa gtgaccaatc cttcttctcg ttattatcaa
    12481 gatgaaactg tcgttcgaac agtcagggat tccatggaat ggatgcataa acatgtctac
    12541 aatagtgaaa agagcattgt tgggaactgg tgggattatg aaatcggtac acctcgtgcc
    12601 atcaacaata ccttgtctct gatgaaagaa tacttctctg atgaggaaat taaaaaatat
    12661 acagatgtga ttgaaaaatt tgtaccagat cccgaacatt tccgaaagac gactgataac
    12721 ccattcaagg ctctaggtgg aaacttagtt gatatgggaa gggtaaaagt aatagctggt
    12781 ttactgcgta aggatgatca agaaatttct tctaccattc gctcgattga gcaagtgttc
    12841 aagttggtag accaaggtga aggtttttat caagatggat cctatatcga ccacaccaat
    12901 gttgcctata cgggtgctta tgggaatgtt ttgattgatg gcctgtctca actgttgcca
    12961 gtcattcaaa agaccaagaa tccaatcgat aaagataaaa tgcaaaccat gtaccactgg
    13021 attgataaat cgtttgctcc tttgctggtg aatggagagt tgatggatat gagtcgtgga
    13081 cgctcgatca gtcgtgcaaa tagcgagggg cacgtggccg cagtagaagt actaagaggg
    13141 attcaccgaa tagcggatat gtctgaagga gaaaccaaac aacgtttgca gagtcttgtg
    13201 aagaccattg ttcaatcgga tagttattat gatgtcttta agaatttgaa gacttataag
    13261 gatatcagtt tgatgcaatc cttgttaagt gatgcaggag tcgcaagtgt tccaagacca
    13321 agttacctat ctgcctttaa caagatggat aaaacagcca tgtacaatgc agagaaaggg
    13381 tttggatttg gcttgtcact cttttccagt cgtaccttga attacgaaca catgaacaag
    13441 gaaaataaac gtggttggta tacgagtgat gggatgttct atctttacaa tggcgatttg
    13501 agtcactata gcgatggcta ctggccaaca gttaatccat ataagatgcc tggtacaaca
    13561 gagacggatg ctaagagagc ggatagcgat acaggtaaag ttttaccgtc tgctttcgtt
    13621 ggaacgagca aactagatga tgccaatgcg acagcaacca tggatttcac caactggaat
    13681 caaacattga ctgctcataa gagctggttt atgctaaagg ataagatcgc ctttttagga
    13741 agcaatatcc aaaacacttc aacagatact gctgcaacta caattgacca gagaaaactg
    13801 gaatcaggta atccatataa agtctatgtc aatgataaag aagcctccct tacagaacaa
    13861 gaaaaggatt atcctgaaac ccaaagtgtc tttttagaat cgttcgattc gaaaaagaat
    13921 attggttact ttttctttaa gaagagttca atcagtatga gtaaggcttt gcaaaaggga
    13981 gcctggaagg atatcaatga aggacagtca gacaaggaag ttgaaaatga atttcttacg
    14041 attagtcagg ctcataagca aaatagagat tcttatggct atatgctcat tcctaacgtg
    14101 gatcgtgcca ccttcaatca aatgataaaa gagttagaaa gtagcctcat cgaaaataac
    14161 gaaacccttc agtctgttta tgatgctaaa caaggagttt ggggcattgt gaaatatgat
    14221 gattctgtct ctactatttc caaccaattc caagttttga aacgtggagt ctataccatt
    14281 cgaaaagaag gggatgaata taagattgcc tactataatc ctgaaaccca ggaatcagct
    14341 ccagatcagg aagtctttaa aaagctagag caagcagctc agccacaagt acagaattca
    14401 aaagaaaagg aaaaatctga agaggaaaag aaccattcgg atcaaaagaa tctccctcag
    14461 acaggagaag gtcagtcaat cttggcaagt ctagggttct tgctacttgg ggcattttat
    14521 ctattccgta gaggaaagaa caactaattt gttcatcatc tagatgaatt acatgaaact
    14581 gtggaaaata gaaaaaatcc gaagaaggcc aaatatcttc ggatttttct tttaaaaaag
    14641 gataagttga tacttatcct tttttacttc attattatgt gactatagcg ttttgaaact
    14701 agaatagaac gttataactg ctaaaagatt tctataaatt catttgattt tcctaatcaa
    14761 tttgttcgta tcctatttca ctccactata ttagtccgtc tcgctccgtg aggtgcgaga
    14821 caaaacaacc acccgctata cgggtgcgcg tcgaaggtta taccaaaaaa actccaaacg
    14881 cgatacaata aaggtgttca atccaattgt aaagcgaaag gagaaaaaat atggcacaaa
    14941 agactcatag tttatcatac acaaagtgga ggtgttctat catattgtgt tcacccctaa
    15001 gtatagacga aaagtcatct ataatcaata tagaagtagt ttagacgaaa tatttcatcg
    15061 cttgtgtagt tataaaggtg ttgaaatgat cgagggtcac ttaatgccag accatgtaca
    15121 catgttagtc agtattccgt cgaggctaag tgtttcaagt tttatggggt atttaaaagg
    15181 caaaagtgca ctcatgatgt ttgacaaaca cgccaatctc aagtacaagt ttgggaatcg
    15241 gcatttctgg gaggaaggtt attatgtaag tccagtaggg cttaatgaag ccacaattaa
    15301 gaaatatagt caagattaaa gaaattatcg agtggatgat ttcttcatga gtatgaaaat
    15361 ttgagaacga gtaaagtatg atatatagta aaatgaaata agaacaggac aaaatcgatc
    15421 aggacagtca aatcgatttt taacaatgtt ttagaagcag aggtgtacta ttccagtttc
    15481 aatctactat agcactagat aaattgagtg taaaagaata tgaggatccc tttagggata
    15541 gtggtaagta ataccaaagt ctcttaaaga ggcaagtgac gagtcaagag caataaggct
    15601 tgaacaacgt gaaagccagc gtctttaggc gctggctgat gatttgggct tatagctctg
    15661 agataaacca cccgttagac gggtagttat gattttatct gagtgtaata tactgttggg
    15721 caatctcgct gatgcggtca aagttgcctt gggaagcgag tttattgagt tcgccaccaa
    15781 ttccaacggc atctgcacca gcagcgaacc attgagggat gttgtttagg ccgactcctc
    15841 cggttaccat tacggaaact tgtgggatcg gtgccttgac tgcagagata tatgctggac
    15901 tgagagtact acctgggaag agtttgatga tttcactacc ggcttcaagt gcagtcgtga
    15961 tctctgtgag ggtaatacag cctggaatgt acggtgtgct gtagagattg cacattttcg
    16021 cagtttcagc atggaaagat ggagaaacaa cgtaatttgc tccagctaga atggcatctc
    16081 tagcagttac ggcatcaagc acagtacctg caccgataca aacactctga tcgtcctgat
    16141 acaagtctac aagttccttg atgatttgtc ctgcatactg attggtatag gcgatttcaa
    16201 tagctttgat accgcccttg atacaagcaa tcgaggcttg tagtccttct tcctttgtat
    16261 ttcctcgaat aacagcgaca attttttgtt tttttagttc aataatcgta tctgatttgg
    16321 tcatgtaatt ctcctaacga atgatatctt gtgcatttgc cagtaaattt tcaatactag
    16381 ttgaggaagt ggagagatgg tctcctggaa gagtgcattt gagagttgcg ctcgcaactg
    16441 caaagtcaat ggtagttttt agggaggaat gatggagtag ttggtaaagg gcaccagata
    16501 taaaggcatc cccgctacca attcgttgat agactgcagt ttttagttgg acagactctt
    16561 caaatagttc ttctagagca taggcttgat agacattttt gtcttgctca tcactagagc
    16621 ggagggtatg gaaaatggcc ttgaaaccat aggcttcttt taaaagtcgc atgcgatttt
    16681 ccacctcttc taggctagca ctgtctcttg gaaacatctc tagattttgg tcatcaatca
    16741 tgagaggatc aataccgaag caatagtcag taaaacgtgc aaacttagaa aattcatact
    16801 tggcttctag gactgaaatc atctttgttc tcagattgag atccattgaa acgacaattc
    16861 ctcggcgctt ggcttcttcc aagagtagga gaaggatcgc acggacctct tgaccgatag
    16921 ctacggtgat tccactaaaa tgaaaatggc taatcccctg aaagagagaa tccatatcta
    16981 gcatgtttgg ccgaatctgg ctgatactcg tatgcttacg atcgtagaaa acttcacttt
    17041 gacgacaacc aaagccgttc tccaaatagt agaggccgat tcgatcgcca agccgacaga
    17101 ttgaactggt atcgatttgg tgctgtttca agaatgtgag aaaacgatct ccaatctcgt
    17161 tggcagggag tgcggtaaaa actttcgttg agatacccag ggcttgcaaa ttacaagcga
    17221 tgttaatttc tgatccgcca aaataagtcg aactggcaac atgatcgccg atactggtgg
    17281 catctaatgg tgaaattcga attaatggtt cgccaaacag taatattttc gtcatccatt
    17341 agttcctttc tggtttagaa tagctttact tggctaatac ttctttcaaa taagcagcaa
    17401 cttcatcatc ttggcagttt tcaaagaagt attcttggaa gtatttgcca gagatggtgt
    17461 ctttgaggaa gtcttggtcg attattttaa ggatggtcat caaatcattg tgggtgattt
    17521 gtttcacctc gtttaagata cgagcgttgc gttgttcagg gattacacgt tctcttgggt
    17581 agccaccgcc catttcttca gcaaataagc gttcaaacat caatttgagg gtcagttctg
    17641 ccccccagcc aaatcctttg gcataaggga tagacaaggc gttaccacca ttgatttgag
    17701 aataaaggta agcgtcagtt gggtccactg ctagaccaca gacaacacca gggaagctgt
    17761 ttaaagcaag catagcccct acacccgtac cacagccggt aacaacaaag tcaactgcct
    17821 ttgtatttaa aaggatggca gccattagtc cgttctgcac ataagttaat tgactttctc
    17881 cttcttctcc acgcatacca tagttaaata attggtagcc ttttttatct gtcgcttctt
    17941 ttagactatc gtaaataatg tgattcttgc tagcttgact attttcattg attaatgcga
    18001 ttttcataag atcttctttc tatttttact caggttgttt tccgatgtag gctaagatac
    18061 cgccatctac atataggatg tggccattga caaaattgct ggcatcacta gcgagaaaga
    18121 cagcagggcc catcaaatct tcagtatttc cccaacgtgc agcaggtgtt tttgcaatga
    18181 tgaactggtc aaatgggtgg cgagaaccat cttcttgcaa ttcacgaaga ggtgctgttt
    18241 gaggagtggc aatataaccc ggtccaattc cgttacattg gatattggct ccaccgtatt
    18301 cagacgcaat gttgcgggtc aacattttca agcccccttt agcagcagca taagcgctaa
    18361 ctgtttcacg tcccagttcg ctcatcatcg aacaaatatt gataatcttt ccatgccctt
    18421 tctttatcat agaaggaata actgcctttg aaacgataaa tggtgcgttt aagtcaatat
    18481 cgatgacctt acggaaatca gcggcgctca tttcgcacat tggaactcgg cggataatac
    18541 cagcgttatt aacgaggatg tcaatgacac caacctcttg ttcgatttgc ttgaccatgg
    18601 cttggatacc gtcctcgtct gtcacgtcac agacatatcc atgggcttgg atgccaactt
    18661 cacgataagc ttcaatccct ttattgacca gatcttgatt gatatcgtta aagacaatag
    18721 tagcgccggc ttcagcgtag gatttggcaa tagcaaatcc aattccataa gaagcgccgg
    18781 tgatgagagc aatttttcct tttaaagaaa actgctcaat tgagaatgat gtatttgtca
    18841 tttgatactt cctttcaaat ttgttaagac ttctctataa ccaaattata ttctagtaaa
    18901 ccggttttgt caataaataa atatttttat ttataaaaag gtttgttttc ctaaaaaaac
    18961 ggaagatttt gaaatttctg tctattaaac tggttaaaaa gataatgaaa gcgctaatat
    19021 ttttttaaaa aaactattga caagataaaa ataaaggatt acaatgggga atataaagta
    19081 aaccggtaaa cctaaaaaga aaggagaaaa gatgaaaatt gtacttgtag ggcatggaca
    19141 ttttgctaca gggatttata gttctttaca attgattgca ggtaatcaag aaaatgtgga
    19201 ggcgattgac tttgtggaag gaatgtcagc agatgaactc aagcaaaaaa tcttacttgc
    19261 aatttcaaat gaagaagaag ttttaatcct aagtgatctc ttgggaggat cgccattcaa
    19321 ggtttcttct accataatgg gagaaaatcc agccaagaca atgaatgttc tctcgggttt
    19381 gaacttagcc atgttaatgg aagcagtctt tgctagaatg gctcatagct ttgatgaggt
    19441 tgttaataaa tcagtagtgg cggcccaggg cggagtcgta aatggtaaag aattgttttc
    19501 aacggatgca gaggaagagg aagaagattt cgaatcgggt atttaaaggg taaaagaatg
    19561 ataaaaaagg ttacgattga aaaaataaaa tcgcctgagc gcttcttaga agtaccactt
    19621 ctgacgaaag aagaagtcgg ccaggcaatc gataaggtta ttcggcagtt agaactcaac
    19681 cttgactatt tcaaggaaga tttcccgacg ccagctacct ttgataatgt ctatccaatc
    19741 atggataaca cggaatggac caatggtttc tggacaggag aactgtggtt ggcttatgaa
    19801 tacagtcaac aggatgcatt taaaaacatc gctcataaaa atgttctttc tttcctggat
    19861 cgtgtcaata agagagtaga attggatcac catgatctcg gcttcttgta cacaccgtct
    19921 tgtatggctg aatataagat aaatggagat ggagaggcta gagaagcaac cttgaaagct
    19981 gcagataagt tgattgaacg ctatcaagaa aaaggtggtt ttattcaagc ttggggagac
    20041 ttgggcaaga aagagcatta ccgtttgatt atcgactgct tgctcaatat ccaactctta
    20101 ttctttgctt atcaagaaac aggcgatcaa aaatactacg atattgcaga aagccatttc
    20161 tatgcttcag ctaataatgt aatccgtgat gacgcttcgt ccttccacac cttctatttt
    20221 gatcctgaga caggtcaacc ctttaaaggt gtaacgagac aagggtatag tgatgattca
    20281 tgctgggcac gtggtcaatc atggggagtc tatggtattc ctttgactta tcgtcactta
    20341 aaagacgagt cctgctttga cttgtttaag ggtgtgacca attatttctt gaatcgtctg
    20401 ccaaaagatt atgtgtccta ttgggatttg atttttaatg atggtagtga tcaatcacga
    20461 gattcttcag caacagctat cgccgtctgt gggattcatg aaatgctaaa acatctccca
    20521 gaggtggatg ctgacaaaga tatttataaa catgctatgc atgccatgct tcgttccttg
    20581 atcgaacatt atgcaaatga tcaatttacc cctggtggga caagtctcct ccacggtgtg
    20641 tactcatggc attcaggtaa aggagtggat gaaggcaata tctggggtga ctactattac
    20701 ctagaagccc ttatccgttt ctacaaagac tggaacctat attggtagga ggagaaatat
    20761 gacaatgcca aatattatta tgacccgtat cgatgaacgg ttgattcatg gacaaggaca
    20821 actttgggta aaatacctag gttgtaatac ggtcattgtt gccaatgacg aagtaagcac
    20881 ggacaagatg caacaaactc tgatgaaaac agttgtgcca gactcagttg ccatgcgttt
    20941 cttccctttg caaaaggtga ttgatatcat tcacaaggct aatcctgctc aaacgatctt
    21001 tatcgttgta aaggatgtga aggacgcttt aaccttggta gaaggtggtg tcactatcaa
    21061 agaaatcaat attgggaaca ttcacaatgc ccctggtaaa gagcaagtga cacgctccat
    21121 cttcctgggt gaagaggaca aggcggccct caaggaattg agccaaactc atcaagtaac
    21181 atttaatacg aaaacaactc caacaggaaa tgatggagct gttcaagtca acattatgga
    21241 ctatatttaa aagaggagat cgttatgtcg attaatgtat ttcaagcgat tttaattgga
    21301 ttatggacag ctttctgttt tagtggaatg ctgttaggaa tttacaccaa tagatgtatt
    21361 gttctgtcat ttggtgtcgg aattattcta ggtgatctgc ctactgctct tgcaatggga
    21421 gctattggtg aattggctta tatgggattc ggtgttggtg ctggaggtac tgttccacca
    21481 aacccaatcg gacctggtat ctttggtacc ttgatggcta tcactagtgc tggtaaagtc
    21541 agtccagaag cggctcttgc cctctctact ccgattgctg tggcgattca attcttacaa
    21601 actttcgcct acactgtacg tgctggtgcg cctgaaacag ctatgaagca cttgaaaaac
    21661 cataatttga agaaatttaa gttcactcta aatgcaacaa tttggttgtt tgcctttatt
    21721 ggatttacct tgggttgctt gggtgccctt tcaatggata ccttgttgaa actcgtagac
    21781 tacattccac cggtattact tacaggtttg acagttgctg gtaaaatgct cccagctatc
    21841 ggttttgcga tgatcttgtc agtgatggct aagaaagagt tgattccctt tgtcttgttg
    21901 ggatatgttt gtgcagctta tctaaacatc ccaacaattg gtattgcaat tgtaggtact
    21961 atctttgctt tgattgaatt ttataacaag ccaaaaacag cggatcatgt ggtagaggag
    22021 gaagcacacg atgactggat ctaacaaatt aacaaaacgt gattatctta aaacgtcttt
    22081 gcgggcattc ttttgtcaaa atggatttaa ctatagtaac tatcaagggt tgggatatgc
    22141 caatgtgatg tatcctgctt tgaaaaaaca ctatggagag gatcaggaag gtttctacca
    22201 agccttggaa gaaaactgtg aattctataa taccaaccca cacttcctgc cttttattac
    22261 cagcttgcat cttgtaatgt tggaaaatgg ccgtccggca aaagaaacac gtagcatcaa
    22321 gatggccttg atgggaccat tggcaggtat tggggattct ctttctcaat tctgtttagc
    22381 tccattgttc tcaaccatcg cagcttcgtt tgctcaagaa ggcttggttg tcggtccaat
    22441 cttgttcttc cttgcgatga atacgatttt aacagcgatt aaattgtcaa ctggtctgta
    22501 tggatacaaa ctaggaacaa ctgtgattga taaactaagc gaacagatgg caacgatttc
    22561 tcgtattgcc aatattatcg gtgtaaccgt aattgctggt ttggcagcga catctgttaa
    22621 gattatggtg ccgattacct ttgctgcagg ggaagttaaa gcagacgcta aacaaagtat
    22681 cgtaagtatt cagggaatgc ttgataaggt tgctccagct cttctaccag ccctatttac
    22741 acttttagtt tactacttga tcaaagaaaa gaaatggaca acatataaac tcgttatttt
    22801 aacagttatc atcggaatta tcggaagctg gcttaagatt atagcttaat ggatacaaca
    22861 ttgttttatg gaatagtgat tgtcttggca gtgagtcccc ttttactgtc aagctttcat
    22921 tctattcgtc agcaaaagtt gcttcgcaaa cagatggagc aacgacaaga gtatttagct
    22981 tctttaacat ctggtgatga agtgttgtta ttgtctggaa ttcatggaaa aatcatttct
    23041 atccaagatg acttggtctc cttgcagatt gcaaaaggtg tggtcatcca tgtagaaaag
    23101 gaaagtgtaa tgggaaagac aaaagaactg ctttttaagt agttcttttt ctggtaatag
    23161 ataatgaaag aagaaagaag acaatttttt gaaagaatcg atggaaacca atgtcgtgat
    23221 tatatcttgt cgcactgttc aaaagactat gagaaagtca agagttccct tgaacgcttg
    23281 atggacaatc gttttatgtt tgatagtcct tgggatatgg agccttgttc aaaaatccat
    23341 caaatccaac cgatggtatg ggatcaagta tttgaagatg atccagaatg ggcttatatg
    23401 ctcaatcgac aagaatatct cttgcagttt atgatagggt atctggtaga aggagataag
    23461 gactatattc aaaagtgcaa gttctttcta tttgattgga ttgagcaggt gagagaattt
    23521 tctcctcaat ccttgatgac tagaaccttg gatacgggta ttcgttcctt tacttggttg
    23581 aaactactct tgctcctctt gaaatttgac ttgctagagg agaaagaact agagaaaatt
    23641 ttggtcagtc tagaaaagca gattgacttt atgaaaagct actatcgcgc caagtacacc
    23701 cttagtaact gggggatttt acaaacaatt ccgatgcttg ctatctatca tttcttttca
    23761 gataagatga acctagaaga agcttaccat tttgcttcaa aggagttgaa acagcaaatt
    23821 gagacacaga ttttaggaga tggaagccag tttgaacagt cgattctcta tcatgtagag
    23881 gtttataaag ccttgctgga tttgtgtctc ttgcttccag acttgcaaga tagttaccaa
    23941 gagttgctgg aaaagatggc gacctatatt caaatgatga caggcttaga tggacggact
    24001 ttggcttttg gtgatagcga ttctacagaa acgacagaaa tgttgagcct gtctgctgtg
    24061 gttttgaacc aggaagacct tcttaacggt ctggatgtta aagttgattt gcttagcctc
    24121 ttgttcctgg ggcgagaaaa ggtcaagcga ctgcaggaat ttgaaaggag agcttggcag
    24181 cctaagtcca tgatctttga agactctgga catgtctgca ttaaggatga acatcgttat
    24241 ctatttttca aaaatggtcc gctaggaagt gcccatagcc atagtgacga gaatagtttt
    24301 tgcttacagt atcaaggaca acctattttc atagatgctg ggcgttattc ttatcgggag
    24361 atatatgagc gttatctctt aaagagtgct tggagtcatt cgacctgcat tgtagatggg
    24421 aaagctccgg aaagaatcac gggatcctgg gaatatgaat actatcctca ctccctgttt
    24481 tgtcaccata aagaaaggga gggaatacat tatattgagg gggcttattg gtcagcagaa
    24541 cctgatttgc cttatcttca caagagaaaa atcctcatgt tggtagagga tgtctggctc
    24601 ttggtagatg acatcaggtg tcaaggtcag catgaggcgt tgactcagtt tatccttgac
    24661 aaggatgtga cctatcaaga tgggaaaatc aatcagttga gactatggag tgaagttgat
    24721 tttgatttgg aagataccat catttctcct aaatacaatg agcttgaaag aagtagcaaa
    24781 ctcaccaagc gccaattctt tgagaatcag atgctggatt ataccatcat tgcgcatgag
    24841 agttttgaaa tcatccgtca ttctgtctac cagacagatg atcgtgaagt ggaaaatgct
    24901 ctggcttttg aagtgaaaaa tgacgaaaca gacaagctga ttctgttatt aagcgaggat
    24961 attggtgtag gtgaaaaatt gtgcctcgtt gacggaacaa aaatgcgtgg aaaatgtcta
    25021 gtatatgata aaataaatga gagaatgatt cgcttgcagt gctagaaata ggcattttga
    25081 atagtgaata tgttataata agtattagta ggaggtgttt tagattggag aagaaactga
    25141 ccataaaaga cattgcggaa atggctcaga cctcgaaaac aaccgtgtca ttttacctaa
    25201 acgggaaata tgaaaaaatg tcccaagaga cacgtgaaaa gattgaaaaa gttattcatg
    25261 aaacaaatta caaaccgagc attgttgcgc gtagcttaaa ctccaaacga acaaaattaa
    25321 tcggtgtttt gatcggtgat attaccaaca gtttctcaaa ccaaattgtt aagggaattg
    25381 aggatatcgc cagccagaat ggctaccagg taatgatagg aaatagtaat tacagccaag
    25441 agagtgagga ccggtatatt gaaagcatgc ttctcttggg agtagacggc tttattattc
    25501 agccgacctc taatttccga aaatattctc gtatcatcga tgagaaaaag aagaaaatgg
    25561 tcttttttga tagtcagctc tatgaacacc ggactagctg ggttaaaacc aataactatg
    25621 atgccgttta tgacatgacc cagtcctgta tcgaaaaagg ttatgagcat tttctcttga
    25681 ttacagcgga tacgagtcgt ttgagtactc ggattgagcg ggcaagtggt tttgtggatg
    25741 ctttaacaga tgctaatatg cgtcacgcca gtctaaccat tgaagataag catacaaatt
    25801 tggaacaaat taaagaattt ttacaaaaag aaatcgatcc cgatgaaaaa actctggtat
    25861 ttatccctaa ctgttgggcc ctacctctag tctttaccgt tatcaaagag ttgaattata
    25921 acttgccgca agttgggttg attggttttg acaatacgga gtggacttgc ttttcttctc
    25981 caagtgtttc gacgctggtt cagccctcct ttgaggaagg gcaacaggct acaaagattt
    26041 tgattgacca gattgaaggc cgcaatcaag aagaaaggca acaagtcttg gattgtagtg
    26101 tgaattggaa agagtcgact ttctaaaatg aaggaaaatg acttgcaatc tctgttaaga
    26161 aataaaataa tcccacctag aacaagctag gtgggattat ttgcctatga aatgagaaat
    26221 tatgggagca agctcctaaa tcaactgttt ttgatctact tctttaacta cttgataaaa
    26281 gttatagaag taggccaaac ttgaaatgat ggttacgact aggaatattg aaaatttcca
    26341 ttggacaggg ttggttaaaa gttgtggaaa ggatatgagg agaaagaaga gggctgcgtt
    26401 gaggacaggt atccgttttg attgtatttt ctcaagtcct ttattgagcg caggaagaaa
    26461 gaggagtagg agtagtaaaa ctgtatgaga aacagctcct gaaataaggg cgaagaaaag
    26521 gaaaatactg ataaaaacat gaatgatcag tagtctagct agtgatttca taaggcacct
    26581 cctaatcctg gtctttttta gctcttgcaa tacgaagtga gtcgacaata tgtatcatca
    26641 ctccgaaaaa gaaagctccc agtatagttt taaaaatatg ttttgtattt agaagagaac
    26701 tgataaaatt tggattttca cttgttaggg tatcaatgag tggaattata aaaaatatca
    26761 ctgttccata aatcgaacct gctttcagac caggataacg taactgtttc ttttcttttt
    26821 tcatgagttt cctcctaatc ctcatcttga tttttcttag tttttgcaat gcgacgggag
    26881 atgaggaact gtatgctcgc tccgaagaaa atagaaccga gaatacttga tacaccattt
    26941 cttatagtga gaagagaatg aaaatagtcc tgaccttcat ctatgagtat actgagaaga
    27001 ggagttataa aaaacatcca tagaccaaag aacaaacctg ctttcagacc tgggtagtgt
    27061 agttgcttgc tttctttctc attcagcata tctggttcaa tgactgtgat gcctgttttt
    27121 ttcatttggt aggtgacata gccagaagcg atgagggcaa tcactaaaat tagaggagga
    27181 tagattagag ccacttcttg agggtattta taggccagaa ggagtggaat aagatttccg
    27241 aaaatcatca gataaaagag gatgataaag acttggttcc caatacgatc ggcctcacgc
    27301 cgtttgtatt cgtcaagggg accagaaata ccgtatgtgc gtttgatcag tttttcagtg
    27361 aaggtttctt ttttcatgag tttgctcctt ttttaaaaat cttcctccca aaagagactg
    27421 ttgaggtcag tttggaggct gcgggcgaga ttgagacaga gttccaaggt tggattgtac
    27481 ttgtcgtttt caatcatatt gatagtctgt ctcgagacac cgatatcctt ggcgagttcg
    27541 agctgggaaa tacccaattc cttgcgaaat tctttcacac gattcatctg ttctcctttt
    27601 tgatttatgt cgtatatatt tgactttatt atagtctttt aaacataaag tgtcaagtat
    27661 ttttgacata ttttttgaag aaatagtagt ctccttgtcc tatttgtctg acaagtgcaa
    27721 gctggtcgga tttgtggtaa aatagataag atatgacaaa agaatttcat catgtaacgg
    27781 tcttactcca cgaaacgatt gatatgcttg acgtaaaacc tgacggtatc tacgttgatg
    27841 cgactttggg cggagcagga catagcgagt atttattaag taaattaagt gaaaaaggcc
    27901 atctctatgc ctttgaccag gatcagaatg ccattgacaa tgcgcaaaaa cgcttggcac
    27961 cttacattga gaagggaatg gtgaccttta tcaaggataa cttccgtcat ttacaggcac
    28021 gtttgcgcga agctggtgtt caggaaattg atggaatttg ttatgacttg ggagtgtcta
    28081 gtcctcaatt ggaccagcgt gagcgtggtt tttcttataa aaaagatgcg ccactggaca
    28141 tgcggatgaa tcaggatgct agtctgacag cctatgaagt ggttaatcat tatgactatc
    28201 atgatttggc tcgtattttc ttcaaatacg gtgaggataa attctctaaa cagattgcgc
    28261 gtaagattga gcaagcgcgt gaagtgaagc cgattgagac aacgactgag ttagcagaga
    28321 ttatcaagtt ggtcaaacct gccaaggaac tcaagaagaa ggggcatcct gctaagcaga
    28381 ttttccaggc tattcgaatt gaagtcaatg atgaactggg ggcggcagat gagtccatcc
    28441 agcaggctat ggatatgttg gctctggatg gtagaatttc agtgattacc tttcattcct
    28501 tagaagaccg cttgaccaag caattgttca aggaagcttc aacagttgaa gttccaaaag
    28561 gcttgccttt catcccagat gatctcaagc ccaagatgga attggtgtcc cgtaagccaa
    28621 tcttgccaag tgcggaagag ttagaagcca ataaccgctc gcactcagcc aagttgcgcg
    28681 tggccagaaa aattcacaag taagagggaa aaagatggca gaaaaaatgg aaaaaacagg
    28741 tcaaatacta cagatgcaac ttaaacggtt ttcgcgtgtg gaaaaagctt tttacttttc
    28801 cattgctgta accactctta ttgtagccat tagtattatt tttatgcaga ccaagctctt
    28861 gcaagtgcag aatgatttga caaaaatcaa tgcgcagata gaggaaaaga agaccgaatt
    28921 ggacgatgcc aagcaagagg tcaatgaact attacgtgca gaacgtttga aagaaattgc
    28981 caattcacac gatttgcaat taaacaatga aaatattcga atagcggagt aagatatgaa
    29041 gtggacaaaa agagtaatcc gttatgcgac gaaaaatcgg aaatcgccgg ctgaaaacag
    29101 acgcagagtt ggaaaaagtc tgagtttatt atctgtcttt gtttttgccg tttttttagt
    29161 caattttgcg gtcattattg ggacgggcac tcgctttgga acagatttag cgaaggaagc
    29221 taagaaggtt catcaaacca cccgtacagt tcctgccaaa cgtgggacta tttatgaccg
    29281 aaatggagtc ccgattgctg aagatgcgac atcttataat gtctatgcgg tcattgatga
    29341 gaactataag tcagcaacgg gtaagattct ttacgtagaa aaaacacaat ttaacaaggt
    29401 tgcagaggtc tttcataagt atctggacat ggaagaatcc tatgtaagag agcaactctc
    29461 gcaacctaat ctcaagcaag tttcctttgg agcaaagggc aatgggatta cctatgccaa
    29521 tatgatgtct atcaaaaaag agttggaaac tgcagaggtc aaggggattg attttacaac
    29581 cagtcccaat cgtagttacc caaacggaca atttgcttct agttttatcg gactagctca
    29641 gctccatgaa aatgaagatg gcagcaagag cttgctggga acctctggaa tggagagttc
    29701 cttgaacagt attcttgcag ggacagacgg cattattacc tatgaaaagg atcgtctggg
    29761 aaatattgtc cccggaacag aacaaatttc ccaacaaacg gtggatggta aggatgttta
    29821 cacaaccatt tccagccccc tccagtcctt tatggaaacc cagatggatg cttttcaaga
    29881 gaaggtaaaa ggtaagtaca tgaccgcgac cttggtcagt gcaaagactg gtgaaatcct
    29941 tgctaccacc caacgaccga cctttaatgc agatactaaa gaaggaatca ctaaggactt
    30001 tgtttggcgt gatatccttt atcaaagtaa ctatgaacca gggtcagcca tgaaggttat
    30061 gacgttagct gcttctattg ataacaatac cttcccaggt ggagaatatt tcaatagcag
    30121 tgaattaaaa atagcggatg tgacgatccg agattgggac gttaatgaag gtttgaccgg
    30181 cggtggtatg atgacctttt ctcaaggatt tgctcactca agtaacgttg ggatgaccct
    30241 ccttgagcaa aagatgggag atgctacctg gcttgattat cttaatcgct ttaaatttgg
    30301 tgttccgacc cgtttcggtt tgacggatga gtatgctggt cagcttcctg cggataatat
    30361 tgtcaacatt gcgcaaagct catttggaca agggatttca gtgacccaga cgcaaatgat
    30421 tcgtgccttt accgctattg ctaatgacgg tgtcatgctg gagcctaaat ttattagtgc
    30481 catttatgat ccaaatgatc aaactgctcg gaaatctcaa aaagaaattg tgggaaatcc
    30541 tgtttctaaa gatgcagcta gtctaactcg gactaacatg gttttggtag ggacggatcc
    30601 ggtttatgga accatgtata accacagcac aggcaagcca actgtaactg ttcctgggca
    30661 aaatgtagcc ctcaagtctg gtacggctca gattgctgac gagaaaaatg gtggttatct
    30721 agtcgggtta actaactata ttttttcggc tgtatcgatg agtccggctg aaaatcctga
    30781 ttttatcttg tatgtgacgg tccaacaacc tgaacattat tcaggtattc agttgggaga
    30841 atttgccaat cctatcttgg agcgggcttc agctatgaaa gactctctca atcttcaaac
    30901 aacagctaaa tctttggagc aagtaagtca acaaagtcct tatcctatgc ctagtgtcaa
    30961 ggatatttca cctggtgatt tagcagaaga attgcgtcgc aatcttgtac aacctattgt
    31021 tgtgggaaca ggaacgaaga ttaaaaacag ttctgctgaa gaagggaaga atcttgcccc
    31081 gaatcagcaa gtcctgatct tatctgataa agcagaggaa gttccagaca tgtatggttg
    31141 gacaaaggcg actgctgaga cctttgctaa gtggctcaat atagaacttg aatttgaagg
    31201 ttcgggcact actgtgcaga agcaagatgt tcgtgctaat acagctatca aggacattaa
    31261 aaaaattaca ttaactttag gagactaata tgtttatttc catcagtgct ggaattgtga
    31321 catttttact aactgtagta ggaattccgg cctttatcca attttataga aaggcgcaaa
    31381 ttacaggcca gcagatgcat gaggatgtca aacagcatca ggcaaaagct gggactccta
    31441 caatgggagg tttggttttc ttaattactt ctgttttggt tgctttcttt tttgccctat
    31501 ttagtagcca attcagcaat aatgtgggaa tgattttgtt catcttggtc ttgtatggct
    31561 tggtcggatt tttagatgac tttctcaagg tctttcgtaa aatcaatgag gggcttaatc
    31621 ctaagcaaaa attagctctt cagcttctag gtggagttat cttctatctt ttctatgagc
    31681 gcggtggcga tatcctgtct gtctttggtt atccagttca tttgggattt ttctatattt
    31741 tcttcgctct tttctggcta gtcggttttt caaacgcagt aaacttgaca gacggtgttg
    31801 acggtttagc tagtatttcc gttgtgatta gtttgtctgc ctatggagtt attgcctatg
    31861 tgcaaggtca gatggatatt cttctagtga ttcttgccat gattggtggt ttgctcggtt
    31921 tcttcatctt taaccataag cctgccaagg tctttatggg tgatgtggga agtttggccc
    31981 taggtgggat gctggcagct atctctatgg ctctccacca agaatggact ctcttgatta
    32041 tcggaattgt atatgttttt gaaacaactt ctgttatgat gcaagtcagt tatttcaaac
    32101 tgacaggtgg taaacgtatt ttccgtatga cgcctgtaca tcaccatttt gagcttgggg
    32161 gattgtctgg taaaggaaat ccttggagcg agtggaaggt tgacttcttc ttttggggag
    32221 tgggacttct atcaagtctc ctgaccctag caattttgta tttaatgtaa gaatggcacc
    32281 ctgatgtttc agggtgtttt tgtatttaaa tacacaatga aaatcaaaga acaaactaga
    32341 aagctaactt taggctgctc aaaatatagt agattgaaac tagaatagta cacatctgct
    32401 tctaaaatat tgttagaaat cgatttgact gtcctgatcg atttgtcctg ttcttatttc
    32461 attttaatat acagttttga gatggctgat agaactgacg aagtcagctc aaaacacggt
    32521 tttgaggttg tagataagga gaagctgatg tggtttgaag aggttttcga agagtgtaaa
    32581 aaatattggt cagttaaagt caaaactctt gaccttttct gaccaatgta gtatattatg
    32641 aacgtaaggt aaatgaaagc cttactagaa cacttattta aagtaaaata gaaagaggtg
    32701 ggtctatgtt tcatctagaa atcttcagaa gtaaagatag tctactcctg cttgaaaaag
    32761 aaaaaccaga aatagtacgt agggtagcga tctagctagt ctaaattttt taaaacaatg
    32821 gtcaaagata gtcaatatca gtaatcataa ctaagtaaac aaaaagaggt aaagaatatg
    32881 aataacaact ttaataattt taataacatg gatgatttat ttaaccaatt gatgggtggt
    32941 atgcgaggat acagttctga aaatcgccgt tacttaatta atggacgcga agtcacacct
    33001 gaggaatttg ctcactatcg tgcgactggt caattaccag gaaatgcaga aactgatgtg
    33061 caaatgccac aacaggcatc aggtatgaaa caagacggtg tccttgcaaa actaggtcga
    33121 aacttaacag cagaagcgcg tgagggcaag ttggatcctg ttatcggacg aaacaaggaa
    33181 attcaagaaa catctgaaat cctctcacgc cgcaccaaga acaatcctgt tttggtcgga
    33241 gatgcaggtg ttggtaagac agcagttgtc gaaggtctag cgcaagccat tgtgaacgga
    33301 gatgttcctg ctgctatcaa gaacaaggaa attatttcta ttgatatctc aggtcttgag
    33361 gctggtactc aataccgtgg tagctttgaa gaaaatgtcc aaaacttagt caatgaagtg
    33421 aaagaagcag ggaatattat cctcttcttt gatgaaattc accaaattct tggtgctggt
    33481 agcactggtg gagacagtgg ttctaaagga cttgcggata ttctcaagcc agctctctct
    33541 cgtggagaat tgacagtgat tggggcaaca actcaagacg aataccgtaa caccatcttg
    33601 aagaatgctg ctcttgctcg tcgtttcaac gaagtgaagg tcaatgctcc ttcagcagag
    33661 aatactttta aaattcttca aggcattcgt gacctctatc aacaacacca caatgtcatc
    33721 ttgccagacg aagtcttgaa agcagcggtg gattattctg ttcaatacat tcctcaacgt
    33781 agcttgccag ataaggctat tgaccttgtc gatgtaacgg ctgctcactt ggcggctcaa
    33841 catccagtaa cagatgtgca tgctgttgaa cgagaaatcg aaacggaaaa agacaagcaa
    33901 gaaaaagcag ttgaagcaga agattttgaa gcagctctaa actataaaac acgcattgca
    33961 gaattggaaa agaaaatcga aaaccacaca gaagatatga aagtgactgc aagtgtcaac
    34021 gatgtggctg aatctgtgga acgaatgaca ggtatcccag tatcgcaaat gggagcttca
    34081 gatatcgaac gtttgaaaga tatggctcat cgcttgcaag acaaggtgat tggtcaagat
    34141 aaggccgtag aagctgtagc tcgtgctatc cgtcgtaacc gtgctggttt tgatgaagga
    34201 aatcgcccaa tcggcagctt cctctttgta gggtctactg gggttggtaa gacggagctt
    34261 gctaagcaat tggcactcga tatgtttgga acccaggatg cgattatccg tttggatatg
    34321 tctgaataca gtgaccgcac agctgtttct aagctaattg gtacaacagc aggctatgtg
    34381 ggttatgatg acaatagcaa taccttaaca gaacgtgttc gtcgcaatcc atactctatc
    34441 attctcttgg atgaaattga aaaggctgac cctcaagtta ttacccttct cctccaagtt
    34501 ctagatgatg gtcgtttgac agatggtcaa ggaaatacag taaacttcaa gaacactgtc
    34561 attattgcga cctcaaatgc tggatttggc tatgaagcca acttgacaga agatgcggat
    34621 aaaccagaat tgatggaccg tttgaaaccc ttcttccgtc cagaattcct caaccgcttt
    34681 aatgcagtca tcgagttctc acacttgact aaggaagatc tttctaagat tgtagatttg
    34741 atgttggctg aagttaacca aaccttggct aagaaagaca ttgacttggt agttagtcaa
    34801 gcggctaaag attatatcat agaagaaggt tacgacgaag tcatgggggt tcgtcctctc
    34861 cgtcgcgtgg ttgaacaaga aattcgtgat aaggtgacag acttccactt ggatcattta
    34921 gatgctaaac atctggaagc agatatggaa gatggcgttt tggttattcg tgagaaagtc
    34981 taagacagaa ttttgaggat aaaaaagaag gagccagctg aaaaaaactg gttccttttg
    35041 aggtatgacg ggcatgtcgt atagtagaag tgtattattc tagtttcaat ttactatagt
    35101 agctcagaag tcggtactta aacgtgctat atcaaaacca gtccttgaaa aacgtggact
    35161 ggtttcgtgt ttggattatt accttgaacg acatgcgtta aaagttagtt gaacagccgt
    35221 atgccgaacg gcacgtacgg tggtgtgaga ggggctagag attatcccct actcgatttt
    35281 aaatcacatg acgttcaaag gcatcatctg aaatcccttg ttccaagatg agttttgccc
    35341 attctttagc agagaagagg ctgtggtcct tgtagtttcc gcaagattcg atggttgtcc
    35401 ctgggacatc ttcccaagta gtagtttcag cgatttcctt gagcgaatcc ttgataacag
    35461 ctgcgatttt agcactggtg tgacgtcccc acataatcat gtggaagcct gtgcggcaac
    35521 caaatggtga acagtcaatc atgccgtcaa tgcgggtacg gatgagtttg gctaagaggt
    35581 gctcgatagt gtgaaggccg gcagtaggga tagagtcttc gtttggttgc accaagcgaa
    35641 tatcataatt ggagatgatg tctccttttg gtcctgtttc ttccccaatc aagcgaacat
    35701 agggtgcttt gacaatggtg tggtcaagtt caaaactttc gacaataact tcttttgaca
    35761 tggtaaatcc tttcagtttt cttctctcat tatatcataa aggttgctcc tgagacagag
    35821 agaaaacctc tccgaggctg gagaggttga aatctttact tacgatataa gcggtcgtat
    35881 tggtagtatg ggtcaaaggt tacgttgata cccagtttac gaaggacatt cttgtcttca
    35941 tcagtcaaga tgatggttga gtgggcttcg cttcctttga ggttgccgag ttcttccata
    36001 gcgcgggcag catcaggatt ttctgtagct gtgatagcaa gtgcaatcag gatttcattt
    36061 gaatgaaggc gtggattgcg gctaccgaga tgatcgattt taagaccttg gattggctta
    36121 acaacttcag gctcgattag ttttacttct ttagcgatgt cagctgattt tttgatggca
    36181 ttgatcaagg cagcggctgt agggccaaag agttctgagt tcttaccagt gatgatttcc
    36241 ccatttggca attcaaaggc tagggctggt ccaccagttt cttctgcttt ttggcgcgca
    36301 acgacagcaa ccttacggtc tgcaggtgtg ataccgaggt cgttcatgag caactcaatt
    36361 ttcttgacgg cagcttcgcc aactttttca gctttgaagt caagaactgt ttgatagtaa
    36421 cggcggatga tttcttgttt agaagcttcg acagcagcct cgtcatctgt aatagcgaaa
    36481 ccaaccatgt tgacacccat atctgtcggt gaagcgtatg gtgattttcc gagaatacgt
    36541 tccaacatgc gcttgagcac tgggaagatt tcgatatcac ggttgtagtt gacagtggtt
    36601 tctccatagg tttgaagatg gaaggggtca atcatgttga catcatcaag gtcagctgtg
    36661 gcagcttcat aagccaagtt aactggatga tgaaggggaa gattccaaac agggaaggtt
    36721 tcaaatttag cgtagccaga tttgatgcca ttgatttggt cgtggtacat attggacata
    36781 cacgttgcca attttccaga accgggtcca ggagcggtta cgacaatcaa gttgcgactg
    36841 gttttgatgt agtcgttttt gcccatgcct tctggggaaa tgatgtgatc catatccgtc
    36901 ggatatcctt tgattggata atgaagataa gaatcaattc cgtttttctc aagttggttg
    36961 cggaaggcat ctgcagcggg ttggccagcg tattgtgtaa tgacaacgga accaacaaaa
    37021 atccctaatt cattgaattt gtcaatcaaa cgaagaactt cttggtcata agaaatgccc
    37081 aagtcgccac gtgctttgga atgttcaatg ttgctagcat taatggcaat cacaacctca
    37141 acctgctctt tcaattcttg caagagcttg attttgttgt caggttcata accaggaagg
    37201 acacgagcag cgtggaaatc ttctaacatt ttgccaccaa actctaggta aagcttgccg
    37261 tcaaattggt taatgcgctc taagatgtgg tcgcgttgta aattcaaata ctgttcagaa
    37321 ctaaaagctt gttttttcat ttttttacct ctggactcta ttataataaa aaattggaag
    37381 ttaggaaact acggagctaa aaaataaatt aaaaagatta agcaaacgct tgcacaaaat
    37441 tttaaaaagt gctatcatat actatagatt attaaaataa tgaggtaaac agatgcaaga
    37501 aaaatggtgg cacaatgccg tagtctatca agtctatcca aagagtttta tggatagtaa
    37561 tggagatgga gttggtgatt tgccaggtat taccagtaag ttggactatc tagccaagct
    37621 aggaatcaca gcaatttggc tttctcccgt ttatgacagc cctatggatg ataatggcta
    37681 tgatattgct gattatcaag cgattgcggc tatttttgga accatggagg acatggatca
    37741 gctgattgca gaagctaaga agcgtgacat tcgtatcatc atggacttgg tggttaatca
    37801 tacctcagat gaacatgctt ggtttgtcga agcctgtgaa aatactgaca gccctgagcg
    37861 agactactat atctggcgcg atgaacccaa tgacctaaat tctatcttta gtgggtctgc
    37921 ttgggaatac gatgaaaagt caggtcaata ctatctccac tttttcagca agaaacagcc
    37981 ggatctcaac tgggaaaatg aaaaacttcg ccagaaaatt tatgagatga tgaacttctg
    38041 gattgataaa ggtattggtg gtttccgtat ggatgttatt gacatgattg gcaaaattcc
    38101 tgacgagaag gtagtcaata atggtcctat gctccatccc tatctcaagg aaatgaatca
    38161 ggcgaccttt ggagataagg atctcttgac agtaggggag acttggggag caacgccagc
    38221 gattgccaag ctttactctg atccaaaggg gcaagaattg tctatggtct tccagtttga
    38281 acatatcggt cttcagtatc aggaaggtca gcctaaatgg cactatcaaa aagagctgaa
    38341 tatcgctaag ttaaaagaaa tcttcaacaa atggcagaca gagttaggag ttgaggacgg
    38401 ttggaattcc ctcttctgga acaatcatga cctccctcgt atcgtctcaa tctggggaaa
    38461 tgaccaagaa taccgcgaaa aatctgccaa agcctttgca atcttgcttc atcttatgag
    38521 aggaactcct tatatctacc aaggtgagga gattgggatg accaactatc cgtttgaaac
    38581 actggatcaa gtagaagata ttgaatctct caactatgcg cgtgaggctc ttgaaaaagg
    38641 tgttccgatt gaagaaatca tggacagtat ccgtgttatt ggacgtgaca atgcccgtac
    38701 ccctatgcaa tgggacgaga gcaaaaacgc tggtttctca acagatcaac cttggttggc
    38761 agttaatcca aattacgaga tgatcaacgt ccaagaagcg ctggcaaatc cagattctat
    38821 tttctatacc tatcagaaac tggtccaaat tcgcaaggag aatagttggc taattcgagc
    38881 tgactttgaa ttgcttgata cggctgataa ggtctttgct tatatacgta aggatggcga
    38941 ccgtcgcttc ctagttgtgg ctaacttgtc caatgaagag caagacttga cagtagaagg
    39001 aaaagtcaaa tctgtcttga ttgaaaacac cctagctcaa gaagtctttg aaaaacaaat
    39061 cttagttcca tgggatgctt tctgtgtgga attactataa atattttttg cagaaaaatt
    39121 taaaattgaa atcatataaa aacaagggag gactgtataa aaggcggaaa tcctttgttt
    39181 tttataacca aggtttataa actttcattc tcgaaattca attaacttta caaattccca
    39241 ctattaagga gaaagaagac gaacataaag aagcgtatcc ttagtgcagg cctgactttt
    39301 gcatctgctt tgcttttagc tgcttgcgac caatcaggtt cagatacaaa aacttactca
    39361 tcaaccttta gtggaaatcc aactacattt aactatctat tagactatta cgctgataat
    39421 atagtcaatt gaaacaagag aaagacaaaa gagcctcgta aaaggtattg caacttggta
    39481 ataccttttt gaggtgcttt ttgatatgag cccatgtttt ctcaatagga ttgtactcag
    39541 gtgagtaggg aggaagaggt aaaagtttat gcccaaactc ttcgcataaa agttctagct
    39601 tccccattct atggaatctt gcattatcca taataataac cgatggtgtg gttaatgttg
    39661 gtaagaaaaa cttctgaaac caagcttcaa aaaagtcgct cgtcatcgtc tcttcgtaag
    39721 tcattggaac gattaactca ccatttgtta gacctgcaac caaagaaatc ctctgatatc
    39781 ttcttccaga tactttgcct cttcttaact gaccttttaa tgagcgacca tattctcgat
    39841 aaaaataagt atcgaatcct gtttcatcaa tctaaacagg tgctaggtgc tttaaactat
    39901 taaaattctt aagaaataag gctacttttt ctgggtcttg ttcataatta gattgaaact
    39961 agaatagtac acctctgctt ctaaaacatt gttagaaatc gatttgactg tcctgatcga
    40021 tttgtcatgt tcttatttca ttttactata tttttgtttc gcgggaagtc tactaagata
    40081 cttaaagatg cagatagtaa aaaaaaatgt agacattacc gtaaaaaagt gatataatcg
    40141 tatgatgttc aaggtatagg tgttaatcat gagtagacgt tttaaaaaat cacgttcaca
    40201 gaaagtgaag cgaagtgtta atatcgtttt gctgactatt tatttattgt tagtttattt
    40261 tttattgttc ttaatcttta agtacaatat ccttgctttt agatatctta acctagtggt
    40321 aactgcgtta gtcctactag ttgccttggt agggctactc ttgattatct ataaaaaagc
    40381 tgaaaagttt actatttttc tgttggtgtt ctctatcctt gtcagctctg tgtcgctctt
    40441 tgcagtacag cagtttgtgg gactgaccaa tcgtttaaat gcgacttcta attactcaga
    40501 atattcgatc agtgttgctg ttttagcaga tagtgagatt gagaatgtta cgcaactgat
    40561 gagtgtgaca gcaccgactg ggactgataa tgaaaacatt caaaaactac tagctgatat
    40621 caaatcaagt cagaataccg atttgacggt caatcagagt tcgtcttact tggcagctta
    40681 taagagtttg attgcaggag agactaaggc cattgtctta aatagtgtct ttgaaaatat
    40741 catcgaatcg gagcatccag attacgcatc gaagataaaa aagatttata ccaagggatt
    40801 cactaaaaaa gtagaagctc ctaagacgtc taaggatcag tctttcaata tctatgttag
    40861 tggaattgat acttatggtc cgattagttc ggtgtcgcgt tcagatgtca atatcctgat
    40921 gactgtcaat cgagatacca agaaaattct cttgaccaca acgccacgtg atgcttatgt
    40981 accaatcgca gatggtggga ataatcaaaa agataaattg acccatgcgg gcatctatgg
    41041 agttgattcg tccatccaca ctttagaaaa tctctatgga gtggatatca attactatgt
    41101 gcgattgaac ttcacttctt tcttgaaatt gatcgatttg ttgggtgggg tagatgttta
    41161 taatgaccaa gattttacat ctctacacgg gaagttccat ttcccagttg gaaatgtcca
    41221 cttagattct gagcaggctc tgggatttgt tcgtgagcgc tattccctag cggatggtga
    41281 tcatgaccgc gggcgcaatc aacaaaaggt gattgcggcc atccttcaaa aattaacttc
    41341 gagcgaagcg ctgaaaaatt atagtaggat cattgatagc ttgcaagatt ctatccaaac
    41401 aaacatgcca cttgagacca tgataaactt ggtcaatgct cagttagaaa gtggtggaac
    41461 gtacaaagtg aattcgcaag acttgaaggg tatgggacgg acggatcttc cttcctatgc
    41521 gatgccagat agtaacctct atatgatgga aattaacgac agtagccttg catctgtcaa
    41581 aactgctatt caggatatgt tggagggcag atgaaatgat tgatattcat tcgcacatcg
    41641 tctttgatgt agatgatggt cccaagtcaa gagaggaaag caaggctctc ttggcagaat
    41701 cctacaggca gggggtgcga accattgtct ctacctctca ccgtcgcaag ggcatgtttg
    41761 aaactccgga agagaagata gcagaaaact ttcttcaggt tcgggaaata gctaaggaag
    41821 tggcgagtga cttggtcatt gcttacgggg ctgaaattta ctacacatca gatgttttgg
    41881 ataagctgga aaaaaatcgg attccgaccc tcaataatag tcgttatgcc ttgatagagt
    41941 ttagtatgaa caccccttat cgcgatattc atagtgcctt gagtaaaata ttgatgttgg
    42001 gaattactcc agtcattgcc cacattgagc gctatgatgc acttgaaaat aatgaaaaac
    42061 gcgttcgaga gctgatcaat atgggctgtt acacgcaagt aaatagttca catgtcctca
    42121 aatccaaact ttttggagaa cgttataaat tcatgaaaaa aagagctcag tattttttgg
    42181 agcgtgactt ggttcatatc attgcaagtg atatgcataa tgtggacagc agacctcccc
    42241 atatggcaga agcatatgac cttgtttccc aaaaatacgg agaagcgaag gctcaggaac
    42301 tttttataga taatcctcga aaaattgtaa tggatcaact aatttaggag aaatgatgaa
    42361 agaacaaaac acgatagaaa tcgatgtatt tcaattactt aaaaccttgt ggaaacacaa
    42421 gctaataatt ttattagtgg cacttgtgac aggggcggga gcttttgcat atagcacttt
    42481 tattgttaag ccagaatata caagcaccac gcgtatttac gtagtcaacc gtaatcaagg
    42541 agataagccg ggactgacga atcaggactt gcaggcagga acttatctgg taaaagacta
    42601 ccgcgaaatt atcctttcgc aagatgtatt ggaaaaggta gcgacaaatt tgaaattgga
    42661 tattccagca aaaacgttaa ctagcaaagt gcaagtgact gtaccagccg acactcgtat
    42721 cgtctcaatc tctgtcaagg ataaacaacc agaggaagcc agtcgcattg ctaattctct
    42781 acgagaagtt gctgcagaaa agatcatcgc tgtaacgcga gtatctgatg taacgacgct
    42841 tgaagaagcg cgaccagcta tgactccctc ttctccaaat gttcgacgca acaccttagt
    42901 tggttttctt ggaggagccg ccgtaacagt aattactgtt cttttgattg agttgctcga
    42961 tacccgtgta aaacgtcctg aagatgttga agatgtactg caaatgccac ttctaggagt
    43021 cgttccagat tttgataaaa tgaaatagga ggaagttatg ccaacgttag aaatctcaca
    43081 ggcgaaatta gaacttgtga aaaaggcaga ggaatattat aatgctttgt gcacgaacct
    43141 acagttaagt ggagatgatt tgaaagtatt ttctatcact tctgtgaaag caggagaagg
    43201 aaaaacaacg acttccacca atatcgcttg ggcttttgcg cgtgcaggtt acaaaacgct
    43261 tctgattgat gcagacatgc gtaactccgt tatgtcaggt gtctttaaat caagggaaag
    43321 gattactgga ctaacagaat ttctatcagg gacaacagac ctgtcacagg ggctttgcga
    43381 taccaatatt gagaatctct ttgtgattca ggctggctct gtgtcaccaa atccgacagc
    43441 ccttcttcaa agtaagaatt tcagtacaat gcttgaaacc ttgcgtaaat attttgacta
    43501 tatcgtcgta gacactcccc ctgtcggtgt cgtgattgat gcggctatca ttatgcagaa
    43561 atgtgatgct tctattttag tgacgaaggc aggtgaaaca aaacgacggg atcttcaaaa
    43621 agcgaaagaa cagttggaac aaactgggaa gtcgtgttta ggagttgtgt tgaataaact
    43681 cgatacttca gtagagaaat acggttctta tgggagttat gggaattaca ggaaacagaa
    43741 aaaataggtt gagggataga aatgagtgaa aaactagcaa agccttcagt agccatagtc
    43801 cagagttttc ttgttatttt attgacttat ctacttagcg ctgtgagaga aacagagatt
    43861 gtttcaacaa cagctattgt actttatatc ctccactatt ttgtctttta tatcagtgat
    43921 tatggacgga atttctttaa aaggggatat ttgattgaac ttgtccagac attgaaatat
    43981 atcctattct ttgcgctagc gattagtatt tctaattttt tcttagaaga tcgatttagt
    44041 atttccagaa gaggcatgat ttacttcctc ttattacatg ttttcttagt ctatgtgcta
    44101 aacccattta tcaagtggta ttggaagcgg gcttatccca actttaaagg aagtaagaag
    44161 attctcctac ttacagcaac ttctcgtgtt gaaaaggtac tggatagatt aatagaatca
    44221 aatgatgttg ttggggagtt ggtagccgtc agtgttttag ataaaccaga ttttcagcat
    44281 gatgatttaa aggttgtagc agagggggag atagtaaatt ttgcgactca tgaggtggtc
    44341 gatgaagtct ttatcaatct tccaagtgaa aaatacaata ttggagagct tgtctctcag
    44401 tttgaaacga tgggaattga tgtaacagtc aatctaaatg ctttcgattg ggcacgtaac
    44461 aagcaaattt gtgagatggc aggactaaat gttgtgactt tttctacaac attttataag
    44521 actagccatg tgattgctaa gcggattatt gatattgtcg gttccctggt aggtttgata
    44581 ttatgtggtc tagtcagtat tgtgctggtt cctttgattc gaaaggatgg gggttctgct
    44641 ttttttgctc agacgcgtat aggaaaaaat ggccgccatt ttacttttta taagtttcgc
    44701 tctatgtgtg tggatgctga ggagaaaaaa agagaactca tggaacaaaa taccatgcag
    44761 ggtggaatgt ttaaggtgga cgatgatcct cgtatcacga aaattggtca ttttatacgg
    44821 aagacgagct tggacgagct accacagttt tataatgttc taaagggaga tatgagtttg
    44881 gtaggtacac gaccaccaac agtggacgag tatgagcact ataccccaga acaaaaacgt
    44941 cgcctaagtt ttaaacctgg cataacaggt ttatggcagg tcagcggacg aagtgaaatc
    45001 aaaaatttcg atgaagttgt caaattagat gtggcctata ttgatggttg gacaatctgg
    45061 aaagatattg aaattttatt gaagacagtt aaggttgtat tgatgaagga tggagcgaaa
    45121 tagttcatat gaatgagaga attcaaatat taggaattac tatagatcca ttaacgatga
    45181 aagaaacagt ggatgctgtt gaacagtatg ttttaaagaa gcaccctttg cacttgatgg
    45241 gagttaacgc ggataaaatt aatcagtgct atgaagatga gaaaatcaaa aaaatcgtta
    45301 atgaatcagg aataattaat gccgacggtg cttcagtagt tttagcgagt aagtttttag
    45361 gaacaccagt tcctgagcga gttgctggaa tagacttgat gaaacattta ctagagttgt
    45421 ctaatgaaaa aggatactcg gtctactttt ttggagcaaa agaagacgtt ttacaagata
    45481 tgttaaaaat ttttgaagaa agatatccag ctctaaatgt tgttggctat agaaatggat
    45541 atttttcgcc tgaagatgaa aaacatattc aggaagatat taaagagaaa aaaccagatt
    45601 ttgtgtttgt aggaattact tctcctaaga aagaatatat tatccaatca ttcatggata
    45661 atggaattaa tgctgtgttt atgggggttg gtggaagttt tgatgtacta tcagggcata
    45721 tcaaacgagc tccattatgg atgcaaaatg ctcacctaga gtggttgttt cgtgtggcaa
    45781 atgagcctaa acgtctcttt aagcgttatt ttgtagggaa tgtttcattt ataagaaaag
    45841 ttttaagagc gaaaagggga tataaacttt gaaccaaaca gaaatagtta aattgattca
    45901 gaaagttgaa ttggatgcta taaaagagtt tcaaaaaatt tgtaaagaga acaatataga
    45961 tttcttcctt cgtgggggga gtgtacttgg ggcagtcaaa tatgatggct ttattccatg
    46021 ggatgatgac atggatattg ctgtgcctcg tgaaggctac gataaacttc caggtatctt
    46081 taaggataga attatcgcag gcaaatatca ggttcttgct tatcaatatt gcgatacttt
    46141 acattgctac tttcctcgct tattcctttt agaagatgaa agaaaacgtt tgggattgcc
    46201 acgaaatacc aatttaggtt tgcatttgat tgatatcatt cctttggatg gtgccccaaa
    46261 tcattcattt ttaagaaagc tttacttcgg taaagtatac tggtatcgtt ttttagcaag
    46321 tttaggaact acctatgttg gagaccatgt ggatatgcat tccgctaagc aaaaactaat
    46381 cattggtttc tttaaaaagc taggatttgc aaaactattc cctcaaaatt atgtgtacag
    46441 acgcttggat aatctctata aaaagtatga ttggaaaaag cagaagtatg ctgggacaat
    46501 taatgcatct ctgtttgcca aagaagtcat gccagtagag atttggggag aaggagtaga
    46561 gaagcctttt gaggatgcct tctttaaagt tcccacggag tatgaccgct atctgaaaag
    46621 actttacgga gaaaactatc ttcacgaaga gcctagtgat gatgaaaaga aatcgcattt
    46681 aggaggacaa taatttgttt tgttatatta ttttgcatta caaagtctta gaggaaacta
    46741 tttcttgtgt taaatcaata aaagaaggca attccaatac aaagcagatc gttattattg
    46801 ataatttttc taataacggt acgggtgaaa aactacaaga gctgtatgag tcagattcag
    46861 agattgatgt cttgattaac catgaaaatg ctggtttcgc tcgaggtaat aatgtagcat
    46921 atcagtttgc taaggaaaag tacaaacctg attttatggt tatcatgaat aatgatattg
    46981 agatagaaac agaagagttt gaaaaaatcg tgacagatat ctatcggaag gaaaaattcc
    47041 atttgttagg accagatatc ttttcgacga cgtatcagct tcaccaaaac ccaaaacggt
    47101 tgacgcatta tacttatgaa gaggttaagg ctctcaatga aaaatttaag aaagggagcc
    47161 aagttagtct agcattaaaa attaaatgtt ggttgaagtc tagtaaagtt cttcggacag
    47221 caatctatca aaataggcgt aaaaagaaat cagtagacta tagaaaacag gtagaaaacc
    47281 caattcttca tggttcgttt attgtatatt ctagagattt tattgagaaa gaggagtatg
    47341 cttttaatcc caataccttc ttctattatg aaacagagat attagattat gaagctgagt
    47401 taaaaggata taagagaatt tatacaccga agattaaggt cttgcaccat caaaatgttg
    47461 caaccaatca agtctataca aacttagtag aaaaaacctt gttttcaaac aaatgcaact
    47521 ttgaatccac tagttatttt ttgaagttga tgaaagaaaa cgagggtgtg taaatgactt
    47581 atttattttt actctgcctg accttattct tattaacttt cttctatttt tttgctttca
    47641 atcaagattt gatagctccg ccagttgtca tgtctgttat gtttctcatt agttctgtat
    47701 ttgcccttat taatgtgcaa aactggaata ttgagtatag tggtttagct tatcttttga
    47761 ttattagtgg cattattgtt ttttcaatgc ctctcttggc tcttaattcg cctagtctaa
    47821 ataccaagat taaagtgacg gatcggctca ttgatatcca attctggaaa atagctctta
    47881 ctattatagt tgacctcgtt attctatatc tttataggag agagattcat aatcttgcac
    47941 ttagccatgg ttatacgggt tcaaattttc agtggttctt tagaaatgct accagttatg
    48001 aaggtgagct aacagtgcga acttcgattc gggtcctcat tcgtatcatt gacgtatctg
    48061 cttatatttt tggatatact tttattaata atttcttcat ttatagtcat aaacgctcta
    48121 aagatttact gctcttagtt ccattcttga tttttatttc taaaacctta ttatctgggg
    48181 gtagattgga tattataaaa attttaattg cgtatgttgt aatggcctat attcagcaaa
    48241 aacgaaaagt tggctgggat aaggtcatct cccataaata tatgagactt ggttttgtag
    48301 gcttgatagc tgggattcct accttttact attctttatt cttatctgga cgttctacga
    48361 ctagaactgt atttgaaagt atttcaacct atttaggagg ttcgattcag cattttaatc
    48421 agtatattca aaatcctatt ggagtagctg aggtttttgg cgatgagtca tttgtagcta
    48481 ttatgaatat tttgggtaat cttggctttg tcaattacaa tagtaccgtt cacttagaat
    48541 ttcggcagtt agggattact atgggtaacg tttatacttt ctttagaaga ccctggcatg
    48601 actttgggtt agtcggtatg tatatctttt cctttgttgt cggtgtgttt tttgctattt
    48661 tttatttgaa attaagaaag agccgtgctg gttttaaatt ggatattcat acaattattt
    48721 actcttattt cttttattgg atttttttat cgtccattga gcaatactcg tttacaacaa
    48781 tcagtttgtt tacacttgta tttatagtct tggtttacct aatggctttc ttctattgga
    48841 atttggattt ccacagagga aaactagtaa ttaaactctc tgacacaagt attaaatcgg
    48901 aaaactgaaa acaataaaaa tagagaggat agttgatgaa tagcaaaatt aaaaatatac
    48961 taactaattt ctcttatgtc atttcttcaa atcttttgac agtcttgacc tcttccttgg
    49021 ttgtcttaat tttccccaaa ttaatggggg taacagagta cagttactgg caactttata
    49081 ttttttatct gacctatatc ggttttttcc acttgggttg gattgatggg atttatctta
    49141 aatatggtgg cttagaatat aataatttag atagaaaaca gttttattct cagatgattc
    49201 tattttctag cttcttaatg attatctcct tgctattgtt tggtttaaat gtattagctg
    49261 taacagattc gaatgcacga tatatttata atatgactat tataagtatg ataatcacaa
    49321 atttaagaac gctctacgtg tatgttttgc agatgacaaa ccgcttgaag gatagttcca
    49381 tcattctact cagtgatcgc gttttatatg tgctcctttt attcctgttt attgtatttg
    49441 gttggcatga atacaaggtt atgattttgg cagatattct aggtcgatca ttttctctca
    49501 tactttcctt ctggatttgt aaagatattg tgtttcagcc tttgtcaaaa ttcatcttta
    49561 atataaaaga gtcttttgat aatatccgag ttggtatcaa tttaatgtta tccaacatcg
    49621 caagtagttt gattatcggt attgttcgtc taggaatcca atggaactgg aatatcgaaa
    49681 cattcgggaa agtatctctg accttgagtg tttccaatct attgatgact ttcattaatg
    49741 ccattggact agtcgtcttt ccgctattaa gacgaacaaa agcagaaaac ttacctaaga
    49801 tttattctaa tttaagaaac gttttgatgc ttatcatgtt cgcgattttg ctcatttact
    49861 atcctttaaa aattgtatta gacctctggt tgccagccta tcaagatgcc ttgattttca
    49921 tgacccttat tttccctatg tcagtctatg aagggaaaat ggcattggtc attaatactt
    49981 acttaaaggc attaagaatg gaaagagata tccttagagt caatgcctta gtaatgttga
    50041 tcagtatggg agtgaccctg gttacaacat acctgttaaa tagtttggag ctgactgttg
    50101 tatcgatagt tgttttgcta gctttgagaa gtatcatagc cgaattaatt ctgtctaaaa
    50161 aactggatgt ttcggttaag aaggatattg tattagaatt tcttttgacg cttgtcttta
    50221 tttcttcaag ttggtatttg ccgattgggc tggcagtaat tgtctacaca atagcctacg
    50281 gtttatatct ctatttgaaa cacgaagata tcaaaaccta tttagcttac tttaaagcta
    50341 gtaaaaaaac atcaaattaa aaaattatat aatgattaag tggtagattc tatttctacc
    50401 gttttagata ttcgggaggt aatgatgaaa aaaattatgt tagtattcgg tacacgtccg
    50461 gaagccatca aaatgtgtcc attagtcaat gagttgaaaa aacacgaaga tatggaaaca
    50521 attgtgtgtg ttactggaca acacaaagag atggttagtc ctgttttaga tttatttggt
    50581 gttgtaccag attatgattt agaaattatg aaggctaacc aaaccttgtt ctctatcaca
    50641 actagtatct tggaaaagat aaaaccagtt ttagagaagg aacaaccaga tattgtccta
    50701 gttcacggtg acactacgac aacttatgca gcagccttgg cagcattcta tttgggaatt
    50761 aaagtaggac atgttgaagc tggtttgcga acgtacaatt tacaaagtcc atttcctgaa
    50821 gaatttaaca ggcaatcgac atcaatcatt gcaacttacc attttgctcc aactgagttg
    50881 gctaaagaaa atctcttaaa agaaggtaga gagaatgttt atgtgactgg aaatactgtc
    50941 attgatgctc ttacaactac tgttcaagag gattatacac acactcattt agatttaaac
    51001 gctaacaatc gtctcatctt attgactgct catagacgcg aaaatctcgg cgaaccgatg
    51061 agacatatgt ttagagcagt taaacgagta ttgaatgaat acgaagatgt taaagtcatt
    51121 tatccaattc ataagaatcc tttggtacgt gaaacagctg cagagatctt tggagataca
    51181 gaacggattc aaattattga acctttggat gttcttgatt tccacaactt catgaaccaa
    51241 agttatatga ttttaacaga ttctggcgga gttcaggaag aagcaccttc tttaggaaaa
    51301 cctgtattgg ttatgcgcga tactactgaa agacctgaag gagtagcggc aggaaccttg
    51361 aaattagtag gaactgacga agaaacaatt tatcaaaact tcaaactact tctagatgat
    51421 tctggagaat acaaaaaaat gagtcaggct agcaatccat acggtaatgg tgatgctagt
    51481 caacagattg ttcagatttt gcgtgggatt taagaagatt ctctaaaaga gtctaagagg
    51541 aatccactcc acaaacataa aactcttatg ctatcattta tgattgagat agactcataa
    51601 cttaatattt ttatcctaag aaaattcgtt catattcaat atgaatgaaa aacaacgaag
    51661 aaaggttatt tcattatgaa aggtattatt cttgcaggcg gctcaggtac ccgcctgtac
    51721 ccacttactc gggctgcgtc aaaacagctg atgccggttt atgataaacc tatgatttat
    51781 tatccgttgt cgacattaat gttggctgga attaaagata ttttgattat ctcaactcct
    51841 caagatttgc cccgttttaa ggacttgctc ttggatggtt ccgaatttgg gatcaagctt
    51901 tcctatgcgg aacaacctag tcccgatgga cttgctcagg cttttcttat cggtgaagaa
    51961 tttatcggtg acgatagcgt tgccttgatt ttaggcgaca atatctacca tggtcctggt
    52021 ctgagcacaa tgcttcaaaa agcagccaag aaagagaaag gtgcgactgt ttttggctac
    52081 caagtgaagg atccagagcg ttttggtgtg gttgagtttg atacagacat gaatgctatt
    52141 tctatagaag aaaaaccgga gtatcctcgc tccaactatg cagtgacagg actgtatttc
    52201 tatgataatg atgttgtgga gattgctaaa cagatcaaac ctagtgctcg tggcgagtta
    52261 gaaattacag acgttaacaa ggcttaccta aatcgtggtg acctttctgt tgagctgatg
    52321 gggcgtggtt ttgcctggtt ggatacggga acccatgaaa gcttgctaga agcttctcag
    52381 tatattgaaa cggttcagcg tatgcagaac gttcaagtgg caaatctaga agaaatttcc
    52441 tatcgtatgg gctacattag tcgtgaagac gtgctggaat tggctcagcc tcttaagaaa
    52501 aatgaatacg gacaatattt gctccgtttg attggagaag catagatgtc agataatttt
    52561 tttggaaaga cacttgtggt acgcaagatt gatgctatac caggactgct agagtttgat
    52621 attcccgttc atggagacaa tcgtggttgg tttaaggaaa atttccagaa ggaaaagatg
    52681 ctaccgcttg gttttcctga aagcttcttt gctgcaggga aactgcaaaa taacgtcagc
    52741 ttttctcgca aaaatgttct tcgaggactc catgctgaac cttgggacaa gtatatctct
    52801 gttgcagacg atgggaaggt tttaggatct tgggtagatt tgcgtgaagg cgagaccttt
    52861 ggaaatgttt accagacaga gattgatgct agcaagggaa tttttgtccc tcgtggcgta
    52921 gccaacggtt ttcaagtcct ctcagatacg gtttcctata gctatctggt caatgactac
    52981 tgggctcttg aactcaaacc caaatatgcc tttgttaact atgctgaccc agcattggga
    53041 attgagtggg aaaacctagc ggaagctgag gtttcagagg cagacaaaca ccatcctcta
    53101 ttaagggatg tcaaaccact tacgaaagac gagttgtaaa aggaaacatt atgactgaat
    53161 acaaaaaaat tatcgtgaca ggtggagctg gttttatcgg ttctaacttt gttcactatg
    53221 tttacaataa ctttacagat gtccatgtga cagtgctgga caagctgact tatgcaggta
    53281 atcgtgccaa tattgaagaa attttaggcg accgtgttga gttagtcgtt ggagatattg
    53341 ctgatgcagc cttggtagat aagttagcag ctgaagcgga tgccattgta cactatgcgg
    53401 cagaaagcca caatgacaat tcgcttaatg acccctcgcc atttatccat accaacttca
    53461 tcggaactta tacactcttg gaagcggctc gtaaatacga ccttcgtttc caccatgtgt
    53521 cgactgacga agtctatggg gatctgccgc tgcgtgaaga tttaccaggt catggggaag
    53581 gaccaggtga gaaatttacg gctgaaacca agtacaatcc aagctcgcct tactcatcga
    53641 ctaaggcggc ttcagacttg attgtcaaag cttgggtgcg ctcatttggt gttaaagcaa
    53701 cgatttctaa ctgttcaaac aactatggtc cttaccagca catcgagaag ttcattccgc
    53761 gccagattac taatatcttg agcggtatca agccaaaact ctacggagaa ggtaagaatg
    53821 tgcgtgactg gattcatact aatgaccatt catcaggtgt ttggacgatt ctgaccaagg
    53881 gtcaagtcgg tgaaacctac ctgattggtg cggatggtga gaagaacaat aaggaagtgt
    53941 tggaactcat cctcaaagaa atgggacagc cagctgatgc ttatgaccac gtaacagatc
    54001 gagctggtca cgacctccgc tatgccatcg atgcaagcaa gctccgtgat gaactaggat
    54061 ggaagccaga gtttaccaac tttgaagcag gtctcaaagc gactatcaag tggtacacag
    54121 ataaccaaga ctggtggaaa gcagaaaaag aagctgttga agccaattat gctaagactc
    54181 aggagattat tacagtataa aaagcaggaa atcgctgctt tttattgcta tattgggaag
    54241 agttacatat tagaaaggtc tagagatgat tttaattaca ggggcaaatg gccaattagg
    54301 aacggaactt cgctatttat tggatgaacg taatgaagaa tacgtggcag tagatgtggc
    54361 tgagatggac attaccaatg aagaaatggt tgagaaagtt tttgaagagg tgaaaccaac
    54421 tttagtctac cactgtgcag cctatactgc tgttgatgca gcagaggatg aaggaaaaga
    54481 gttggacttc gccatcaatg tgacggggac agaaaatgtc gccaaagcat ctgaaaagca
    54541 tggtgcaact ctagtttata tttctacgga ctatgtcttt gacggtaaga aaccagttgg
    54601 acaagagtgg gaagttgatg accgaccaga tccacagaca gaatatggac gcactaagcg
    54661 tatgggggaa gagttagttg agaagcatgt ttctaatttc tatattatcc gtactgcctg
    54721 ggtatttgga aattatggca aaaacttcgt ttttaccatg caaaatcttg cgaaaactca
    54781 taagacttta acagttgtaa atgaccagta cggtcgtccg acttggactc gtaccttggc
    54841 tgagttcatg acctacctag ctgaaaatcg taaggaattt ggttattatc atttgtcaaa
    54901 tgatgcgaca gaagacacaa catggtatga ttttgcagtt gaaattttga aagatacaga
    54961 tgtcgaagtc aagccagtag attccagtca atttccagcc aaagctaaac gtccgctaaa
    55021 ctcaacgatg agcctggcca aagccaaagc tactggattt gttattccaa cttggcaaga
    55081 tgcattgcaa gaattttaca aacaagaagt gagataagta gtagaatgat tttctagtct
    55141 aataaaagag gcagataatg aactcaaaag gagcttaagg tgtacgatta tcttgttgtt
    55201 ggtgctggtc tctttggtgc agtctttgcc catgaagtag ccttaaaagg aaaaaagtaa
    55261 aagtcattga aaaacggaat catatcgtgg gtaatatcta tactcgtgaa gaggaaggaa
    55321 ttcaagttca tcaatatggt gctcatatct tccatacttc tgataaggag agctgggatt
    55381 atgtaaatca gtttgcaggg tttaaccgtt acaccaattc tcctgttgca aactataagg
    55441 gtgagattta taaccttcct tttaatatga atactttcaa taaactctgg ggagttgtaa
    55501 cgccagcaga agcacaagct aagattgagg aacaacgtgc tattttaaat ggtaaaactc
    55561 ctgaaaattt ggaagaacag gcgatttctc ttgtaggtac agacatctac gaaaaattaa
    55621 tcaaagacta tacagagaaa cagtggggca aaccaactac tgaacttcca tcctttatta
    55681 ttcgccgttt accagtacac ctgacctatg ataacaacta ttttaacgat acctatcaag
    55741 ggattccaat tggtggatac actcaaatag ttgaaaaatg ttggattatg aaaatattga
    55801 tgtagaaaca aatgttgatt tctttgtgaa caaagagcaa tatctgaaag attttcctaa
    55861 gattgtcttt actggtatga ttgatgaatt ctttgactat aagttgggcg aactagagta
    55921 ccgtagtctt cgttttgaaa atgagacctt ggatatggaa aattaccaag gaaatgcagt
    55981 tgtgaactat acggatgcag aaaccccata tactcgcatt attgaacaca aacattttga
    56041 gtttgggagt caagcaaaga ctatcattac taatagaaca ttctaaaaca tgggaaaaag
    56101 gtgatgagcc ttattatcca gttaataatg atcgtaataa tcatttgtat aaatcatata
    56161 aaaaacttgc tgatgagcaa gggaatgtta tctttggtgg ccgcttagga cactatcgtt
    56221 attacgatat gcaccaagta attggagcag ctttgcagtg cgtgagaaat gagttagatt
    56281 catactcaat gaaaatcaaa gagcaaacta ggaagctagc cacaggttgc tcaaaatact
    56341 gttttgaggt tgcagatgga agctgacgcg gtttgaagag attttcgaag agtataaaca
    56401 agtaaaactg actaccagtt attatttaga aatagtatta aaa
//
LOCUS       DAVHEX010000006        56177 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVHEX010000006 DAVHEX010000000
VERSION     DAVHEX010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 56177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56177
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.006"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(190..423)
                     /locus_tag="VVL45_000368"
     CDS             complement(190..423)
                     /locus_tag="VVL45_000368"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4186604.1"
                     /translation="MFSQLHMREEQVLLAQDYALETARAEGLEQGLERGKVEGRAERK
                     LFAFLDIVRQGLLTSEVASQQLGMSVSEFEALL"
     gene            complement(416..532)
                     /locus_tag="VVL45_000369"
     CDS             complement(416..532)
                     /locus_tag="VVL45_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEU4186605.1"
                     /translation="MVGVFRQQALYPATATSHYPSKSTAGLQELVRGGQENV"
     gene            complement(747..1097)
                     /locus_tag="VVL45_000370"
     CDS             complement(747..1097)
                     /locus_tag="VVL45_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEU4186606.1"
                     /translation="MLRHPGISPTNDLIAKKIFSNPEITCQFIRDMLDLPAKNVTILE
                     GSDIHVLLSMPYSVQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWAYLCSQVN
                     QNLEKIRQREGDTH"
     gene            1353..3986
                     /gene="polA"
                     /locus_tag="VVL45_000371"
     CDS             1353..3986
                     /gene="polA"
                     /locus_tag="VVL45_000371"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEU4186607.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNVAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYDLAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVSESLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFVWSCGDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIMVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYVQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDDVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            4071..4508
                     /locus_tag="VVL45_000372"
     CDS             4071..4508
                     /locus_tag="VVL45_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEU4186608.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
     gene            4549..4626
                     /locus_tag="VVL45_000373"
                     /pseudo
     CDS             4549..4626
                     /locus_tag="VVL45_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(4827..5837)
                     /locus_tag="VVL45_000374"
     CDS             complement(4827..5837)
                     /locus_tag="VVL45_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEU4186609.1"
                     /translation="MSFLSKNGAGILACLLISIVSWYLGGFFPVIGAPVFAIFIGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVVLLGFGLNISQVFAVGQSSLPVILSTISIALI
                     IAYLFQRFFALDTKLATLVGVGSSICGGSAIAATAPVIDAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNEGFALFAGTAVNDTSSVTAAASAWDSLYQSNTLESATIVKLT
                     RTLAIIPITLFLSYWQSRQQENKQSLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKELSKFLIVMAMSAIGLKTNLVAMVKSSGKSILLGAICWIAIILTTLGMQTLIG
                     IF"
     gene            5986..7155
                     /locus_tag="VVL45_000375"
     CDS             5986..7155
                     /locus_tag="VVL45_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU4186610.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQAISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPAAFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            7152..7922
                     /gene="recO"
                     /locus_tag="VVL45_000376"
     CDS             7152..7922
                     /gene="recO"
                     /locus_tag="VVL45_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEU4186611.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKCMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDKRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            7919..8911
                     /gene="plsX"
                     /locus_tag="VVL45_000377"
     CDS             7919..8911
                     /gene="plsX"
                     /locus_tag="VVL45_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEU4186612.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMDLLKTAITGGGLRAKLGALLLKDSLRGLKKQIN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETEVVAQTASEFSGE"
     gene            8917..9150
                     /locus_tag="VVL45_000378"
     CDS             8917..9150
                     /locus_tag="VVL45_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU4186613.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQSVGDVVKIIQGK"
     gene            9196..9486
                     /locus_tag="VVL45_000379"
                     /pseudo
     CDS             9196..9486
                     /locus_tag="VVL45_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            9689..9919
                     /gene="blpU"
                     /locus_tag="VVL45_000380"
     CDS             9689..9919
                     /gene="blpU"
                     /locus_tag="VVL45_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HEU4186614.1"
                     /translation="MNTKKMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGVARGLQ
                     LGIKTRTWQGAATGAAGGAILGGVAYAATCWW"
     gene            10635..12788
                     /locus_tag="VVL45_000381"
     CDS             10635..12788
                     /locus_tag="VVL45_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEU4186615.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEMGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPYKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTVINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLIVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLIALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            12801..14150
                     /locus_tag="VVL45_000382"
     CDS             12801..14150
                     /locus_tag="VVL45_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEU4186616.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAIETGASLAGQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            14320..15027
                     /locus_tag="VVL45_000383"
     CDS             14320..15027
                     /locus_tag="VVL45_000383"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEU4186617.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIAGDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQGLK"
     gene            15084..18809
                     /locus_tag="VVL45_000384"
     CDS             15084..18809
                     /locus_tag="VVL45_000384"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEU4186618.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNVGLSSLKSIRIVQVYDV
                     FDLAEDLFAPAEKHIFSEQGTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITTGIAKQEFS
                     ESDKTIPKLTFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDAFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTVFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPLVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANSTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            18902..20344
                     /locus_tag="VVL45_000385"
     CDS             18902..20344
                     /locus_tag="VVL45_000385"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEU4186619.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSMTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            20381..21403
                     /locus_tag="VVL45_000386"
     CDS             20381..21403
                     /locus_tag="VVL45_000386"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEU4186620.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVACTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLVSSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            21400..21945
                     /gene="purN"
                     /locus_tag="VVL45_000387"
     CDS             21400..21945
                     /gene="purN"
                     /locus_tag="VVL45_000387"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEU4186621.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKTDYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            22029..22538
                     /locus_tag="VVL45_000388"
     CDS             22029..22538
                     /locus_tag="VVL45_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU4186622.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLISFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            22563..24110
                     /gene="purH"
                     /locus_tag="VVL45_000389"
     CDS             22563..24110
                     /gene="purH"
                     /locus_tag="VVL45_000389"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEU4186623.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     ETIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAANENQIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYTVVLDELSAN
                     GETTYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQAMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDRPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIAVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLDGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFT
                     GVRHFRH"
     gene            24232..25494
                     /gene="purD"
                     /locus_tag="VVL45_000390"
     CDS             24232..25494
                     /gene="purD"
                     /locus_tag="VVL45_000390"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEU4186624.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVPTATYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGVIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELSQQKIEGLFYRTDIGSKAIK"
     gene            25897..26385
                     /gene="purE"
                     /locus_tag="VVL45_000391"
     CDS             25897..26385
                     /gene="purE"
                     /locus_tag="VVL45_000391"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEU4186625.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            26372..27463
                     /gene="purK"
                     /locus_tag="VVL45_000392"
     CDS             26372..27463
                     /gene="purK"
                     /locus_tag="VVL45_000392"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEU4186626.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLADKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGEPLPVIKLHAPAVMLNVLGQHVEAAEQYVTENSSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVDELGEGIDF"
     gene            27473..27700
                     /locus_tag="VVL45_000393"
     CDS             27473..27700
                     /locus_tag="VVL45_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HEU4186627.1"
                     /translation="MIQIIVNTFIEKDKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIEKEEFE"
     gene            27763..29061
                     /gene="purB"
                     /locus_tag="VVL45_000394"
     CDS             27763..29061
                     /gene="purB"
                     /locus_tag="VVL45_000394"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEU4186628.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCNKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(29116..33054)
                     /gene="strH"
                     /locus_tag="VVL45_000395"
     CDS             complement(29116..33054)
                     /gene="strH"
                     /locus_tag="VVL45_000395"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEU4186629.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAVLQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKAEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEADKPETEAGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKKSARTVDLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGGMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRY
                     TAESVTAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEALKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLNQLKRIVDKASELGYSDVHLLLGNDGLRFLLDDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYILGQKPEDGGG
                     FLKKAIENTGKTPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQKEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEIPSQPAPSTAPA
                     EESKALPQDPAPVVTEKKLPETGTHDSAELVVAGLMSTLAAYGLTKRKED"
     gene            complement(33369..34085)
                     /locus_tag="VVL45_000396"
     CDS             complement(33369..34085)
                     /locus_tag="VVL45_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU4186630.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCNRDH"
     gene            34433..36220
                     /locus_tag="VVL45_000397"
     CDS             34433..36220
                     /locus_tag="VVL45_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEU4186631.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFEGDLDLDKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEERGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            36217..36693
                     /locus_tag="VVL45_000398"
     CDS             36217..36693
                     /locus_tag="VVL45_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEU4186632.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            36721..37626
                     /locus_tag="VVL45_000399"
     CDS             36721..37626
                     /locus_tag="VVL45_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU4186633.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            37613..38428
                     /locus_tag="VVL45_000400"
     CDS             37613..38428
                     /locus_tag="VVL45_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU4186634.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            38435..38839
                     /locus_tag="VVL45_000401"
     CDS             38435..38839
                     /locus_tag="VVL45_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU4186635.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            39137..40303
                     /locus_tag="VVL45_000402"
     CDS             39137..40303
                     /locus_tag="VVL45_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU4186636.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVANPVT
                     YLKKDVATVLVSFARSGNSPESVATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTALLVFDPTEFAVKSERFEVVSSLARKVLDKA
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            40419..41456
                     /locus_tag="VVL45_000403"
     CDS             40419..41456
                     /locus_tag="VVL45_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEU4186637.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHVYNGTLLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            41573..42786
                     /locus_tag="VVL45_000404"
                     /pseudo
     CDS             41573..42786
                     /locus_tag="VVL45_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein C"
     gene            43238..48808
                     /gene="zmpC"
                     /locus_tag="VVL45_000405"
     CDS             43238..48808
                     /gene="zmpC"
                     /locus_tag="VVL45_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease ZmpC"
                     /protein_id="HEU4186638.1"
                     /translation="MSRKSIGEKRHSFSMRKLSVGLVSVTVSSFFLMSQGIQSVSADN
                     MESPIHYKYMTEGKLTDEEKSLLVEALPQLAEESDDTYYLVYRSQQFLPNTGFNPTVG
                     TFLFTAGLSLLVLLVSKRENGKKRLVHFLLLTSMGVQLLPASAFGLTSQILSAYNSQL
                     SIGVGEHLPEPLKIEGYQYIGYIKTKKQDNTELSRTVDGKYSAQRDSQPNSTKTSDVV
                     HSADLEWNQGQGKVSLQGEASGDDGLSEKSSIAADNLSSNDSFASQVEQNPDHKGESV
                     VRPTVPEQGNPVSATTVQSAEEEVLATTNDRPEYKLPLETKGTQEPGHEGEAAVREDL
                     PVYTKPLETKGTQGPGHEGEAAVREEEPAYTEPLATKGTQEPGHEGKATVREETLEYT
                     EPVATKGTQEPEHEGEAAVEEELPALEVTTRNRTEIQNIPYTTEEIQDPTLLKNRRKI
                     ERQGQAGTRTIQYEDYIVNGNVVETKEVSRTEVAPVNEVVKVGTLVKVKPTVEITNLT
                     KVENKKSITVSYNLIDTTSAYVSAKTQVFHGDKLVKEVDIENPAKEQVISGLDYYTPY
                     TVKTHLTYNLGENNEENTETSTQDFQLEYKKIEIKDIDSVELYGKENDRYRRYLSLSE
                     APTDTAKYFVKVKSDRFKEMYLPVKSITENTDGTYKVTVAVDQLVEEGTDGYKDDYTF
                     TVAKSKAEQPGVYTSFKQLVTAMQSNLSGVYTLASDMTADEVSLGDKQTSYLTGAFTG
                     SLIGSDGTKSYAIYDLKKPLFDTLNGATVRDLDIKTVSADSKENVAALAKAANSANIN
                     NVAVEGKISGAKSVAGLVASATNTVIENSSFTGKLIANHQDSNKNDTGGIVGNITGNS
                     SRVNKVRVDALISTNARNNNQTAGGIVGRLENGALISNSVATGEIRNGQGYSRVGGIV
                     GSTWQNGRVNNVVSNVDVGDGYVITGDQYAAADVKNASTSVDNRKADRFATKLSKDQI
                     DAKVADYGITVTLDDTGQDLKRNLREVDYTRLNKAEAERKVAYSNIEKLMPFYNKDLV
                     VHYGNKVATTDKLYTTELLDVVPMKDDEVVTDINNKKNSINKVMLHFKDNTVEYLDVT
                     FKENFINSQVIEYNVTGKEYIFTPEAFVSDYTAITNNVLSDLQNVTLNSEATKKVLGA
                     ANDAALDNLYLDRQFEEVKANIAEHLRKVLAMDKSINTTGDGVVEYVSEKIKNNKEAF
                     MLGLTYMNRWYDINYGKMNTKDLSTYKFDFNGNNETSTLDTIVALGNSGLDNLRASNT
                     VGLYANKLASVKGEDSVFDFVEAYRKLFLPNKTNNEWFKENTKAYIVEMKSDIAEVRE
                     KQESPTADRKYSLGVYDRISAPSWGHKSMLLPLLTLPEESVYISSNMSTLAFGSYERY
                     RDSVDGVILSGDALRTYVRNRVDIAAKRHRDHYDIWYNLLDSASKEKLFRSVIVYDGF
                     NVKDETGRTYWARLTDKNIGSIKEFFGPVGKWYEYNSSAGAYANGSLTHFVLDRLLDA
                     YGTSVYTHEMVHNSDSAIYFEGNGRREGLGAELYALGLLQSVDSVNSHILALNTLYKA
                     EKDDLNRLHTYNPVERFDSDEALQSYMHGSYDVMYTLDAMEAKAILAQNNDVKKKWFR
                     KIENYYVRDTRHNKDTHAGNKVRPLTDEEVANLTSLNSLIDNDIINRRSYDDNREYKR
                     NGYYTISMFSPVYAALSNSKGAPGDIMFRKIAYELLAEKGYHKGFLPYVSNQYGAEAF
                     ASGSKTFSSWHGRDVALVTDDLVFKKVFNGEYSSWADFKKAMFKQRIDKQDNLKPITI
                     QYELGNPNSTKEVTITTAAQMQQLINEAAAKDITNIDRATSHTPASWVHLLKQKIYNA
                     YLRTTDDFRNSIYK"
     gene            49164..50018
                     /locus_tag="VVL45_000406"
     CDS             49164..50018
                     /locus_tag="VVL45_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4186639.1"
                     /translation="MTYEYQSHIYLAEAVLNVKDLVSQTVFYQQIIGLEILSQTDTEV
                     VLGLGGKALVHLIQAQEGGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEALYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEVLAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSRQFYQTVLGLEDKFSVPSASWIAAGDYHHHLAVNEW
                     GGKGLDPRKQVLPGLAYYVIEVAHKEELLTIAQRAQEVDAPIKWMTSIQLEITDSDGI
                     VTRIRLAR"
     gene            50124..50681
                     /locus_tag="VVL45_000407"
     CDS             50124..50681
                     /locus_tag="VVL45_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEU4186640.1"
                     /translation="MTSEYQKMIAGEFYRPSDPELRALAQASRQKQAAFNKEENPLKG
                     AEIIKTWFASTGKNLYINTRLMVDYGVNIHLGENFYSNWNLTMLDICPIRIGDNAMIG
                     PNCQFLTPLHPLDPQERNSGIEYGKPITIGDNFWTGGGVIVLPGVTLGNNVVAGAGAV
                     ITKSFGDNVVLAGNPARVIKEIPVK"
     gene            50898..51662
                     /locus_tag="VVL45_000408"
     CDS             50898..51662
                     /locus_tag="VVL45_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEU4186641.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGANCVGEFVSATKTYPVYVVNYKDEEICLVQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPSRYMEMQPEAIAAIERVLD
                     DRGIPYEEVMTWSTDGFYRETAEKVAYRKEEDCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            52229..53608
                     /locus_tag="VVL45_000409"
     CDS             52229..53608
                     /locus_tag="VVL45_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEU4186642.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLI
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            53622..54287
                     /locus_tag="VVL45_000410"
     CDS             53622..54287
                     /locus_tag="VVL45_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEU4186643.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            54422..54580
                     /locus_tag="VVL45_000411"
     CDS             54422..54580
                     /locus_tag="VVL45_000411"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4186644.1"
                     /translation="MKIKDQTRKLATGCLKHCFEVVDRTDEVSSKHCFEVADRTDEVS
                     NIYTARRC"
     gene            54642..54815
                     /locus_tag="VVL45_000412"
     CDS             54642..54815
                     /locus_tag="VVL45_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEU4186645.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGWDQVGNFSKGQYIMMHS
                     LIYRTALLRASQF"
     gene            54882..55292
                     /locus_tag="VVL45_000413"
     CDS             54882..55292
                     /locus_tag="VVL45_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEU4186646.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKCIDQQLKVNRLLVDQLD
                     LSQVSHPKMREYLLNHIEITTVISSTLLNRSETAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            55408..>56177
                     /gene="pavB"
                     /locus_tag="VVL45_000414"
     CDS             55408..>56177
                     /gene="pavB"
                     /locus_tag="VVL45_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HEU4186647.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASLAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRA"
BASE COUNT        16186 a        10550 c        13143 g        16298 t
ORIGIN      
        1 taaaatctgg acattagggt ctttaatgtc tagtaatttt gtgataaaat gtaattgttc
       61 catatgattc tttctaatga gttgttttgt cgcttttcat tataggtcat atgggacttt
      121 ttttctacac aaaataggct ccataatatc cataggggat ttacccacta tggatattat
      181 ggagccattt tacaacagtg cctcaaattc agatactgac atacctaatt gctggctggc
      241 aacctcagaa gtcagaagac cttggcgtac tatgtctagg aaggcaaaaa gtttcctttc
      301 tgcccttcct tcaactttcc cacgctctag tccttgttca aggccttcag ccctagcagt
      361 ttccaaggca tagtcctgtg ctaacaagac ttgttcttct cgcatatgta gttgactaaa
      421 cattttcctg tcctcctcgg accagctctt gtagtccagc agttgatttg cttgggtaat
      481 ggctcgttgc ggttgctggg taaagggctt gttgccgaaa aactccaacc atggcttgcg
      541 aaccttgtct ttgctggttt ctctgtattt tttaattcca agaatgccat cttaaccaga
      601 tggttttcct gtccattgtt ggtaatcgcc aatacctcac ctgttgtgtc ttcgcgcata
      661 ctaaagctat gaaaagccag gtcatctgag aaataattac tatccacgat agcaatggcg
      721 taaacaggag cgatgtgttt gtagctctag tgagtatcac cttctcgctg acgaattttt
      781 tcaagatttt gattaacctg actgcacagg taagcccaca agtgattgat gaaaaaattc
      841 tgatgatgga cttgaatctc aataattact tgagtaccgt tatccaactc cgccaagacg
      901 tctatactgg tataaaaatc ctgcaccgag taaggcatgg agagtaatac gtgaatatcg
      961 cttccctcca aaatggtcac attttttgct ggcaagtcca gcatatcgcg aataaattga
     1021 caagtgattt ctggattgct gaatattttc ttagcaatca aatcattagt tgggctgatg
     1081 cccggatgtc tgagaatcct cctattcctc cttctatagt gagttgaaat aagatgtgaa
     1141 taactctatc aggaaagtca aattaattta tagaaatatt ttagcagtca aggtgtacta
     1201 ttatagattc aatatactat attatagcac atttttaaat ctaggtttac taaattctct
     1261 ggctctatct atttattcgg aaaaagtttg aaaaatactt gctctagctc ttcccaatgg
     1321 tattttttga ttctttcctt tataatgggt gtatggataa gaaaaaatta ttattgattg
     1381 atgggtcttc tgtagctttt cgggcgtttt ttgcgctgta tcagcagttg gaccgtttta
     1441 agaatgtggc tggtttgcat accaatgcga tttatggttt tcagttgatg ttgagtcatt
     1501 tattggagcg ggttgagccg agtcatattt tggtggcttt tgatgcggga aagacgacct
     1561 tccggacaga gatgtatgcg gactataagg gtggtcgggc caagactcct gatgagtttc
     1621 gtgagcaatt tcctttcatt cgtgagttgc tggatcatat ggggattcgt cactatgatc
     1681 tggctcagta tgaggcggat gacatcattg ggacgctgga taagctagca gagcaggatg
     1741 gttttgatat tactattgtc agtggggaca aggatttgat tcagctgacg gatgagcata
     1801 cggtggttga aatttccaag aaaggtgtgg ctgagtttga ggcctttacg ccagattacc
     1861 tcatggaaga aatgggcctc acaccagctc agtttatcga tctcaaggcg ctcatgggtg
     1921 ataagtcgga taatatccct ggggtgacca aagtcggtga aaagacgggt attaagctct
     1981 tgctggagca tggttcgctt gaggggattt atgaaaatat tgatggaatg aagacttcta
     2041 agatgaagga aaatctcatc aatgacaagg aacaggcctt tttgtctaaa acactagcga
     2101 ccattgatac caaggcaccg attgcgattg gtttagagga cttggtctat agtggtccag
     2161 atgttgaaaa tcttgggaaa ttctacgatg agatgggctt caaacagcta aagcaggctt
     2221 taaatgtgtc gtcagctgat gtgtctgaga gtttggattt tactattgtt gaccaaatca
     2281 gtcaagatat gctgagtgaa gagtctatct tccactttga gctttttggt gagaattacc
     2341 atacggataa tttggttgga tttgtctggt cttgtgggga taagctctat gccacagaca
     2401 agcttgagct gttgcaagac ccgattttca aggatttctt agaaaaaaca tctctgagag
     2461 tttatgactt taagaaggtt aaagttcttt tgcaacgttt tggtgtggat ttgcaggcgc
     2521 ctgcttttga catccgtttg gctaaatacc tcctttcgac tgtggaggac aatgaaattg
     2581 cgaccatcgc tagtctttat ggtcagactt acttggttga tgatgaaact ttctacggta
     2641 agggtgttaa aaaggccatt cctgaacgtg agaaattctt ggaacactta gcttgtaaac
     2701 ttgctgtttt ggtagaaaca gagcctattt tacttgaaaa actcagcgaa aatgggcaat
     2761 tagagcttct ttatgatatg gagcaacctc tggcttttgt ccttgccaag atggaaattg
     2821 ctgggattat ggtcaagaaa gagaccttgc ttgagatgca ggctgaaaat gagcttgtca
     2881 ttgaaaaact gactcaagag atttacgagc tggctggtga ggagtttaat gtcaactcgc
     2941 ctaagcagtt gggcgtgctt ctctttgaga aattgggact tcctctagaa tacactaaga
     3001 aaaccaagac aggttattcg acagcagtgg atgttttaga gcgtctcgct cctattgctc
     3061 cgattgttaa gaaaatcctg gattaccgtc aaattgctaa gattcaatct acttatgtaa
     3121 ttggcttgca ggactggatt ttggctgatg gaaagattca tactcgctat gtgcaggatt
     3181 tgacccagac cgggcgtttg tctagtgtgg atccaaactt gcaaaatatt cctgcccgat
     3241 tggaacaggg gcgcttgatt cggaaggctt ttgtgccaga gtgggaggat agtgtgctac
     3301 tcagctctga ctattcacag attgaattgc gcgttttggc gcatatttct aaggatgagc
     3361 acttgattaa ggccttccaa gagggggcag atatccatac ttcgacagcc atgcgggtct
     3421 ttggcattga gcgtcctgat gatgtgactg caaacgaccg tcgcaatgcc aaggcagtta
     3481 actttggagt ggtttatggg atttcagact ttggcttgtc taataatttg ggaattagtc
     3541 gtaaggaagc caaagcctac attgatacct actttgaacg ttttccaggt attaaaaact
     3601 acatggatga agtggtgcgg gaggcgcgtg ataagggcta tgtagagacc ctctttaagc
     3661 gtcgccgtga gttgccagat atcaattcgc gcaacttcaa tattcgtggt tttgcggagc
     3721 gaactgctat caactcacct atccagggtt cggcagcaga tattctcaag attgccatga
     3781 ttcagctgga taaagccttg gttgcaggtg gttatcagac taagatgctg ttacaagtgc
     3841 acgatgaaat cgtccttgaa gtgcctaaat ctgaattggt agagatgaaa aaattggtga
     3901 aacaaaccat ggaagaagcc attcaactca gtgttcctct tatcgcagat gagaatgaag
     3961 gggcaacctg gtacgaggct aaataaaaag ggggctagtc ctcctttttt gtagtataat
     4021 tctgcaaacc ttttcaaaat gtgctatact gatgaaaaag gaggatttct atgagtcaag
     4081 aatttatcaa tccaagtgat ggcgtgattc gtcagtatct cgcaacgagt aaaacccttg
     4141 ctgtggtggg tttgtctgac cgtgaagaaa caaccagcaa tcgagtaaca aaggaaatgc
     4201 aggctcgggg ctataaaatc atcccagtca atcccaaggc ggcaggtggc gaaatcttgg
     4261 gtgaaaaggc ttatgctagc ctcgctgaaa ttccttttcc tgtagatatt gtcaatgttt
     4321 atcgtcgcag tgaatttctg cccgatgtgg cgcgtgattt tctcaaagct gatgccaaga
     4381 ttttttgggc acagctagga cttgaaagtt tagaagcgaa agagattttg cgtgatggtg
     4441 gatgtgatga tatcgtgatg aatcgctgta tcaagagaga acatactcgc ttgattgagg
     4501 aagcataaga aaaaggtagc tgatgggcta ccttttgtgt tatactcaat gaaaatcaaa
     4561 gagcaaacta ggaaactagc cacgggctgc tcaaaacact gttttgaggt tgcagataga
     4621 actgacgaag tcagctcaaa acactgtttt gaggttgtag atacgactga cgaagtcagc
     4681 tcaaaacacc gttttgaggt tgcagataga actgacgaag tcagctcaaa caccgttttg
     4741 aggttgcaga tagaactgac gaagtcagta acatatacct acggcaaggc gacgttgacg
     4801 tggtttgaag agattttcga agagtattag aaaatgccga taagggtctg cataccaaga
     4861 gtggtgagga tgatggcaat ccagcagatg gctccaagaa gaatggattt tccactggat
     4921 ttgaccatag cgaccagatt ggttttgaga ccgatagcac tcatggccat gacaataagg
     4981 aatttagaga gttctttgag aggagtaaag aaactactgg acacacctag agaggtgagt
     5041 agtgtagtga ggagagaggc aaggataaag taaaggataa aaagtgggaa gacttttttc
     5101 agttgcaggc tttgcttgtt ttcttgttgg cgactttgcc agtaggatag aaagagcgtg
     5161 atagggataa tggccaaagt acgtgtgagt ttaacaatgg ttgcagactc gagggtattg
     5221 ctttggtaaa gactgtccca agcgctggcg gcagccgtta cagaggaagt gtcgttgacc
     5281 gcagtccctg caaagagggc gaagccttca ttggatagat gaagccaggt gccgagggtt
     5341 ggaaagatga gcgcagccaa gacattgaag aaaaagataa cggaaatggc ttgggctact
     5401 tccttttcct tagcatcaat aacgggcgct gtcgctgcaa tggcagaacc cccacagata
     5461 gaagaaccta ctccaaccaa ggtagccagt tttgtatcca gggcaaagaa acgctggaag
     5521 aggtaggcaa taatcagagc tattgagata gtggacagga tgacagggag tgaagattgg
     5581 ccaactgcga agacctgcga gatattgaga ccaaaaccaa gcaagacaac ggcatattgg
     5641 agcaacttct tggaactaaa ggtcaaacca gcatccagtt gtttatagga cgagagaaag
     5701 ggatgtagga gcatgcctat gaaaatggca aaaacgggcg cgccaatcac agggaagaat
     5761 cctcctaagt accaagatac gatagaaatg agaaggcagg ccaagatacc tgctccattt
     5821 tttgatagaa atgacataaa aacctccaaa taaaatctat taccattata gacctataaa
     5881 caggaaaagt aaaatagaaa gtggaaagct attctctaat gtatttttgc ggtcgggggg
     5941 cttttgtagt ataatagaga tacgttttga aagtaggagg tatctatgga cttaactaag
     6001 cgctttaata aacagttaga taaaattcaa gtttcgttga ttcgtcagtt tgaccaggct
     6061 atttcggaga ttcctggggt cttgcgtttg accttggggg aacctgattt tacaacgcca
     6121 gaccatgtca aggaggcggc caagcgagcg attgatcaga accaatccta ctatacaggg
     6181 atgagtggtc tgctgactct acgtcaggca gctagtgact ttgttaagga aaagtaccaa
     6241 ctggactatg ctcctgaaaa tgaaatcttg gttacaattg gggcgacaga ggctttatct
     6301 gcgactttga cggctatttt ggaagaggga gacaaggtac ttttgccagc tcctgcttat
     6361 ccaggctatg aaccgattgt taacttagtt ggggcagaaa ttgttgagat tgatacgact
     6421 gaaaatggtt ttgtcttgac tcctgagatg ttggagaagg ccattttgga gcagggtgat
     6481 aagctcaagg cggttattct caactatcca gccaatccga caggaattac ctacagtcga
     6541 gagcagttag aggccttggc agctgtttta cgcaagtacg aaatttttgt tgtctgtgat
     6601 gaggtttact cagaattgac ctacacaggc gaagcccatg tgtctctagg aacgatgttg
     6661 agagaccagg ctattattat caatggtttg tctaaatcgc atgccatgac aggttggcgt
     6721 ttggggctga ttttcgctcc tgcggccttc acagcccagt taatcaagag tcaccaatac
     6781 ttggtcactg ccgcaaatac catggcgcaa catgctgcgg tagaagcctt gacggctggt
     6841 aaaaatgatg cggagcccat gaagaaggaa tatatccagc gtcgggacta tatcatcgaa
     6901 aaaatgactg ctcttggttt tgagattatc aaaccagacg gtgccttcta tatttttgct
     6961 aaaattccag cgggctacaa tcaagactcc tttgcttttc tgaaggattt tgctcagaag
     7021 aaggccgttg cctttatccc tggtgcagcc tttggacgtt acggggaagg ctacgtccgc
     7081 ctatcttatg cagccagcat ggagactatc aaagaagcca tgaaacgact tgaggagtat
     7141 atgagagaag catgattcag tctatcacga gtcaaggctt ggtgctttac aatcgcaatt
     7201 ttcgtgagga tgacaagctt gtcaaaattt ttacagagca ggttggcaaa tgcatgtttt
     7261 ttgtcaaaca cgctggtcag tctaagctgg cgcctgttat tcagcccttg gtgctggcac
     7321 gatttctctt gcgaatcaat gatgacggac tcagttacat cgaagactat catgaggtca
     7381 tgacttttcc caagattaat agtgacctct ttgtcatggc ctatgcgacc tatgtggcag
     7441 ctcttgcaga tgctagtttg caggacaatc agcaggatgc tcccttgttt gcttttttgc
     7501 aaaagacttt ggagttgatg gaagcaggct tggattatca ggttttgacc aatatttttg
     7561 aaattcaaat tttgactcga tttggaatca gcctcaattt taatgagtgt gtcttctgcc
     7621 atcgggttgg tcaggctttt gacttttctt tcaaatatgg agcctgcctc tgtccagagc
     7681 attatcatga ggataagaga cgttgtcatc tcaatcccaa tatcccctat ctgctcaatc
     7741 aatttcaagc tattgatttt gagactttgg agaccatttc gctcaagcct ggaatcaagc
     7801 aagagctacg ccaatttatg gatcaattat atgaagagta cgttgggatt cacctaaaat
     7861 caaagaaatt tattgattcc ctagcagact ggggacaatt actaaaagag gaaaagaaat
     7921 gaaaaaaatc gcagtagatg ccatgggggg cgattacgca cctcaggcca ttgttgaggg
     7981 tgtcaatcaa gccctatctg acttttcaga tatcgaggtt caactttacg gagatgaagc
     8041 taaaatcaag caatatctga cagcgacaga gcgcgtcagc attatccata cggatgagaa
     8101 gattgattcg gatgatgaac ctacgagagc tattcggaat aagaaaaatg ccagtatggt
     8161 attggcagcc aaggctgtca aagatggtga agcagacgct gtcctttcgg ctgggaatac
     8221 aggtgccttg ttggcagcag gattcttcat cgtgggtcgt atcaagaata tcgaccgtcc
     8281 tggactcatg tctaccttgc ctaccgttga tggaaaaggt tttgacatgc tagaccttgg
     8341 tgccaatgca gaaaatacag cccagcacct ccatcaatat gcggttctag gttccttcta
     8401 tgctaaaaat gtccgtggca ttgcgcaacc acgcgttggt ttgctcaaca acggaacaga
     8461 gagtagcaag ggcgacccgc ttcgtaagga aacttatgaa ttactggcgg ctgatgaaag
     8521 tttgaacttt atcggaaacg tggaagcacg tgatttgatg aatggcgttg cggatgttgt
     8581 tgtggcagat ggtttcacgg gaaacgctgt gctcaaatct attgaaggga cagccatggg
     8641 aattatggac ttgctcaaga ctgctattac aggtggtggt cttcgagcga aactaggtgc
     8701 actccttctc aaggacagcc tcagaggttt gaaaaaacag atcaactatt cagatgttgg
     8761 tggagcagtc ttgtttggtg ttaaggcacc tgttgtcaag actcatggct caagcgatgc
     8821 caaggctgtt tatagtacga ttcgccagat tcgtaccatg ctagaaacag aagtagttgc
     8881 ccagactgcg agtgaatttt caggagaata aaagagatga cagaaaaaga aatttttgac
     8941 cgtattgtga ccattatcca agagagacag ggagaggatt ttgtcgtgac agaatccttg
     9001 agtctgaaag acgacttgga tgctgactca gttgatttga tggagtttat cttgacgctg
     9061 gaggatgaat ttagtatcga aatcagcgat gaggaaattg accaactcca aagtgtagga
     9121 gatgtggtta aaatcattca aggaaaatag caatcggagt tccaagtcaa cggaagtaga
     9181 tggtttttag aaatgagaaa tatcggacaa gctggtaaaa tcttggctga cagtggttat
     9241 caagggctca tgaagatata tcctcaagca caaactccac gtaaatccag caaactcaag
     9301 ccgctaacag ttgaagataa agcctgtaac catgcgctat ctaaggagag aaacaaggtt
     9361 gagaatatct ttgccaaagt aaaaacgttt aaaatgtttt caacaaccta tcgaaatcat
     9421 cgtaaacgct tcggattacg aatgaatttg attgctggta ttatcaatca tgaactagga
     9481 ttctagtttt gcaggaagtc taatagtaaa aaagtgatta gaaaacatct tttttaaaaa
     9541 tagagatgat tttgaaacaa aaaagctaat tcaagacgtt tcgatgccaa ttcaagattt
     9601 ggatgaaaaa aattaataga tactgttata ctaaacttgt caagtttgta acaagacaaa
     9661 tattaaaaat aaaaaagagg tattcgttat gaatacaaaa aagatgtcac aatttgaaat
     9721 tatggatact gagatgcttg cttgcgttga aggtggcgga tgcaattggg gagattttgc
     9781 caaagcaggt gttggaggag gagtagcacg aggtcttcag ctaggaatta aaacaagaac
     9841 atggcaaggt gcagcaactg gtgctgcggg aggagctata cttggaggtg tggcctatgc
     9901 agcgacatgt tggtggtaat tatggatttt aaaagtttta ttattggttt agtagttggt
     9961 atatttggtc cttatatgga tgatttaatt agaaaaaaat ttttaaagtc ttcggagaag
    10021 aaaacagaaa aatctgttaa aaaataatca aaactataaa tgatgaatct gaatcaaaat
    10081 tattttgcgc atgtaaagag gagtcttata gtaacgagtc aaaaaaggag taactatgaa
    10141 tcgtaattta gaacggtgtt acctattctg actatggata gatcatacca gaggcggctt
    10201 agaaatagca gggacattag aaattgaagt aacttttaat aggatgtcgt aagtgttact
    10261 atcaatgatt tatttgtttc aagcttgcct agggtgacag taaaaaatca atttcctttc
    10321 aatagcatat ttttagtggg cagtactctt gttctgccta tttttttatc caaaaagtgc
    10381 agttgggagg gagataggct catttgggaa ggaagtccag tttttgttta gtgattgggg
    10441 taagatagtt gttatcagat gagtttatac tcttcgaaaa tcaaattcaa accacgtcaa
    10501 cgtcgccttg ccgtatatat gtgactgact tcgtcagtct tatatacaac ctcaaaacag
    10561 tgttttgagc agcctgcggc tagtttccta gtttgctctt tgattttcat tgagtttagg
    10621 aaaaggagat gaatatgaaa tttgggaaac gtcactatcg tccgcaagtg gatcagatgg
    10681 actgcggtgt agcttcatta gccatggttt ttggctacta tggtagttat tattttttgg
    10741 ctcacttgcg agaattggct aagacgacca tggatgggac gacggctttg ggcttggtca
    10801 aggtggcaga ggagatgggt tttgagacgc gagccattaa ggcggatatg acgctttttg
    10861 acttgccgga tttgactttt ccttttgttg cccatgtgct taaggaaggg aaattgctcc
    10921 actactatgt ggtgactggg caggataagg atagcattca tattgccgat ccagatcccg
    10981 gggtgaagtt gactaaactg ccacgtgagc gttttgagga agaatggaca ggagtgactc
    11041 tttttatggc acctagtcca gactataagc cttataagga acaaaaaaat ggtctgctct
    11101 cttttatccc tatattagtg aagcagcgtg gcttgattgc caatatcgtt ttggcaacac
    11161 tcttggtaac cgtgattaac attgtgggtt cttattatct gcagtctatc attgatacct
    11221 atgtgccaga tcagatgcgt tcgacactag ggattatttc tattgggcta gtcatcgtct
    11281 acatcctcca gcaaatcttg tcttacgctc aggagtatct cttgcttgtt ttggggcaac
    11341 gcttgtcgat tgacgtgatt ttgtcctata tcaagcatgt ttttcacctc cctatgtcct
    11401 tctttgcgac acgcaggaca ggggagatcg tgtctcgttt tacagatgct aacagtatca
    11461 tcgatgcgct ggcttcgacc atcctttcga ttttcctaga tgtgtcaacg gttgtcatta
    11521 tttcccttgt tctattttca caaaatacca atctcttttt catgacttta ttggcgcttc
    11581 ctatctacac agtgattatc tttgccttta tgaagccgtt tgaaaagatg aatcgggata
    11641 ccatggaagc caatgcggtt ctgtcttctt ctatcattga ggacatcaac ggtattgaga
    11701 ctatcaagtc cttgaccagt gaaagtcagc gttaccaaaa aattgacaag gaatttgtgg
    11761 attatctgaa gaaatccttt acctatagtc gagcagagag tcagcaaaag gctctgaaaa
    11821 aggttgccca tctcttgctt aatgtcggca ttctctggat gggggctgtt ctggtcatgg
    11881 atggcaagat gagtttgggg cagttgatta cctataatac cttgctggtt tactttacca
    11941 atcctttgga aaatatcatc aatctgcaaa ccaagcttca gacagcgcag gttgccaata
    12001 accgtctaaa tgaagtgtat ctagtagctt ctgagtttga ggagaagaaa acagttgagg
    12061 atttgagctt gatgaaggga gatatgacct tcaagcaggt tcattacaag tatggctatg
    12121 gtcgagacgt cttgtcggat atcaatttaa tcgttcccca agggtctaag gtggcttttg
    12181 tggggatttc agggtcaggt aagacgactt tggccaagat gatggttaat ttttacgacc
    12241 caagtcaagg ggagattagt ctgggtggtg tcaatctcaa tcagattgat aaaaaagccc
    12301 tgcgccagta catcaactat ctgcctcaac agccctatgt ctttaacgga acgattttgg
    12361 agaatcttct tttgggagcc aaggagggga cgacacagga agatatctta cgggcggtcg
    12421 aattggcaga gattcgagag gatatcgagc gcatgccact gaattatcag acagaattga
    12481 cttcggatgg ggcagggatt tcaggtggtc aacgtcagag aatcgctttg gcgcgtgctc
    12541 tcttgacaga tgcgccggtc ttgattttgg atgaggcgac tagcagtttg gatattttga
    12601 cagagaagcg gattgtcgat aatctcatag ctttggacaa gaccttgatt ttcattgctc
    12661 accgcttgac tattgctgag cggacagaga aggtagttgt cttggatcag ggcaagattg
    12721 tcgaagaagg aaagcatgct gatttgcttg cacagggtgg cttttacgcc catttggtca
    12781 atagctagaa agaggagagg atgaaaccag aatttttaga aagtgcggag ttttataatc
    12841 gtcgttacca taatttttcc agtagtgtga ttgtacccat ggcccttctg ctcgtgtttt
    12901 tacttggctt tgcaactgtt gcagagaagg agatgagttt gtccactaga gctactgtcg
    12961 aacctagtcg tatccttgca aatatccagt caactagcaa caatcgtatt cttgtcaatc
    13021 atttggaaga aaataagctg gttaagaagg gggatctttt ggttcaatac caagaagggg
    13081 cagagggtgt ccaagcggag tcctatgcca gtcagttgga catgctaaag gatcaaaaaa
    13141 agcaattgga gtatctgcaa aagagcctgc aagaagggga gaaccacttt ccagaggagg
    13201 ataagtttgg ctaccaagcc acctttcgcg actacatcag tcaagcaggc agtcttaggg
    13261 ctagtacatc gcaacaaaat gagaccatcg cgtcccagaa tgcagcagct agccaaaccc
    13321 aagccgaaat cggcaacctc atcagtcaaa cagaggctaa aattcgcgat taccagacag
    13381 ctaagtcagc tattgaaaca ggtgcttcct tggccggtca gaatctagcc tactctcttt
    13441 accagtccta caagtctcag ggcgaggaaa atccccaaac taaggttcag gcagttgcac
    13501 aggttgaagc acagatttct cagttagaat ctagtcttgc tacttaccgt gtccagtatg
    13561 caggttcagg tacccagcaa gcctatgcgt cagggttaag cagtcaattg gaatccctta
    13621 aatcccaaca cttggcaaag gttggtcagg aattgaccct tctagcccag aaaatcttgg
    13681 aggcagagtc aggtaagaag gtacagggaa atcttttaga caaggggaaa attacggcaa
    13741 gtgaggatgg ggtgcttcat cttaatcctg agaccagtga ttctagcatg gttgcagaag
    13801 gtgccctact agcccaactt tatccatctt tggaaagaga agggaaagcc aaactcacag
    13861 cctatctaag ttcaaaagat gtagcaagaa tcaaggtcgg tgattctgtt cgctatacta
    13921 cgactcatga tgccgggaat caactttttc tagattctac tattacaagt attgatgcga
    13981 cagctactaa gactgagaaa gggaatttct ttaaaatcga ggcggagact aatctaactt
    14041 cggagcaggc tgaaaaactt aggtacgggg tggaaggccg tctacagatg attacgggca
    14101 agaaaagtta cctacgttat tatttggatc aatttttgaa caaagagtaa tgttcgtgtt
    14161 tttagagtta aataattttt aaactgtgag aaagattctt cttgcagttt tttctttaca
    14221 atttttgaaa aacatctgct atttattcgg ttgaattctt gtgttttttg gttttttgtg
    14281 gtaaaatgtg ctcaagtaat acgaaaggcg aactttaaaa tgtcaaaaca attgatctat
    14341 tcgggaaaag ctaaagatat ctatacaact gaggatgaaa atcttattat ttcaacttac
    14401 aaggaccagg cgactgcttt caacggtgtc aagaaggagc agattgcagg taagggagtc
    14461 ttgaataatc agatttcatc ttttattttt gagaaattaa atgcggctgg tgtggcgact
    14521 cactttgtgg agaaactttc agacacggaa caactcaata aaaaggttaa gattattcct
    14581 ttggaagtcg tgctccgcaa ctatactgct ggttcctttt caaaacgttt tggtgtggat
    14641 gagggaatcg ccttggagac tccgattgtc gaattttact acaaaaatga tgatttggat
    14701 gatccattta tcaatgatga gcatgtgaaa ttcctacaga ttgcgggtga ccagcagatt
    14761 gcctacttga aggaagaaac gcgtcgtatc aatgaactat tgaaagtctg gtttgctgag
    14821 attgggctta aattgattga ctttaagcta gagttcggtt ttgacaagga tggcaagatt
    14881 atcttggcag acgaattttc accagataac tgccgcttgt gggacgctga tggcaaccac
    14941 atggataagg atgttttccg tagaggattg ggagaactaa ccgacgttta tgagattgtt
    15001 tgggaaaagt tgcagggttt aaaataacaa cctcaaggct gtttgggaat attgcaagag
    15061 ctgaaataaa ggaataagaa ttgatggata aacgtatttt tgttgaaaaa aaggctgatt
    15121 ttcaggtcaa gtcagagagt ttggttagag agctccagca caacgtggga ctgtcaagct
    15181 tgaaaagtat tcgtattgtg caagtatatg atgtatttga cttggctgag gacttgtttg
    15241 cacctgcaga gaagcacatt ttctctgagc agggaactga ccatgtttta gatgaagtat
    15301 ctgtgcaggc ggatcttgct aactatgctt tctttgccat tgaaagtctg ccagggcagt
    15361 ttgaccagcg tgcagcttcg tcacaggaag ccttgctttt gttgggaagt tcgagtgacg
    15421 tgacagtcaa cacagcccaa ctttacttgg tgaataaaga tattgatgcg actgagttgg
    15481 aagctgtcaa aaactacctg ctcaatccag ttgattctcg tttcaaggat atcacgacag
    15541 ggattgccaa gcaggagttt tcagagtcag acaagaccat tccaaaattg actttctttg
    15601 aaagctatac agcagaagac tttgctcgct acaaggccga gcaagggatg gccatggaag
    15661 tggatgattt gctctttatc caagactact ttaagtcaat cgggcgcgtg ccaactgaga
    15721 ctgaactcaa ggttttggac acttactggt ctgaccactg ccgtcatacg acttttgaga
    15781 cagagttgaa acacatcgac ttttcagctt ctaaatttca aaagcaattg cagtcaacct
    15841 atgacaagta tattgccatg cgcgaggagt taggtcggtc tgaaaaacca caaaccttga
    15901 tggatatggc gactattttc ggtcgttatg agcgtgctaa tggacgattg gatgatatgg
    15961 aagtctctga cgaaatcaat gcctgctcag ttgaaattga agtggatgtt gatggtgtca
    16021 aggaaccttg gctcctcatg tttaaaaatg aaacccacaa ccatccaaca gaaattgagc
    16081 catttggtgg agcggctacc tgtatcggtg gagctattcg tgatccgttg tcaggccgtt
    16141 cctatgttta ccaagccatg cgtatttcag gtgctggtga tattacagca ccgatttcgg
    16201 aaactcgtgc tgggaaattg ccacaacaag tcatttctaa aacagcagct catggttatt
    16261 cttcatatgg taaccagatt gggcttgcca caacctacgt tcgtgaatac ttccacccag
    16321 gctttgtagc taaacgtatg gaacttggtg ccgttgttgg tgcggctccc aagggcaatg
    16381 ttgtccgtga aaaacctgaa gcaggtgatg tgatcatcct tctcggaggc aaaacaggtc
    16441 gtgatggtgt cggtggtgcg acgggctctt ctaaggttca aacagttgag tctgtagaga
    16501 ctgctggtgc tgaggttcaa aaaggaaatg ccatcgaaga acgcaagatt cagcgcctct
    16561 tccgtaatgg caatgtcact cgtctgatca agaagtccaa tgactttggg gcaggcggcg
    16621 tctgtgtggc tatcggtgaa ttggcagacg gtcttgaaat cgacctcaac aaggtgcctc
    16681 ttaaatacca gggcttgaat ggtacagaaa ttgccatctc tgaatcacaa gaacggatgg
    16741 cggtcgtggt tcgtcctgaa gatgtggatg ccttcgttgc cgaatgtaac aaagaaaata
    16801 ttgatgctgt tgtggtggcg acagtaactg aaaaaccaaa tcttgtcatg cactggaatg
    16861 gtgagacaat cgttgacttg gagcgtcgtt tccttgacac caatggtgtg cgcgtggttg
    16921 tcgatgccaa agttgtggac aaggatgtca aactcccaga agagcgtcaa acatctgctg
    16981 aaacactgga atcagatacc cttacggttc tatctgacct caaccatgca agtcaaaaag
    17041 gattacagac tgtctttgac tgctctgttg gacgctcaac ggttaatcac ccacttggtg
    17101 gtcgttacca actcacacca actgaggcat ctgtgcagaa attgccagtt caacacggtg
    17161 tgactcatac tgcgtcggtc attgctcaag gtttcaaccc atatgtagct gaatggtctc
    17221 cataccacgg tgctgcttac gcggttatcg aagcaactgc tcgtttggtg gctgctggtg
    17281 ccaactggtc taaggctcgt ttctcttacc aagagtattt cgagcgtatg gataaacaag
    17341 cagagcgttt tggtcagcca gtagccgccc ttctaggctc tattgaagct cagattcaac
    17401 ttggcttgcc atctatcggt ggtaaggact ccatgtctgg tacctttgaa gaattgaccg
    17461 ttccgccaac cttggttgcc tttggggtga cgacggcaga tagccgtaag gtgctctctc
    17521 cagaatttaa agctgttggg gaaaatatct actacatccc aggtcaagcc ctctctgcag
    17581 agattgattt tgacttgatt aagaaaaatt ttgctcagtt tgaagcaagc caagctgacc
    17641 ataaagtgac atctgcatca gctgtcaaat acggtggtgt agttgaaagt ttggctcttg
    17701 ctacctttgg aaactatatt ggtgcagagg tgaccttgcc tgaacttaaa acagctttga
    17761 cagctcaatt aggcggcttt gtcttcacat ctcctgaaga aattgctgga gtagagaaga
    17821 ttggacaaac gaaagcagac tttacactga ctgtcaacgg tgtgaagcta gatggacaca
    17881 agcttgacag tgcatttcaa gggacattgg aagaagttta cccaacagaa tttacccaag
    17941 cgaaagaact agaagaagta ccagctgtgg catcagatgt tgtgattaaa gccaaagaaa
    18001 aggttgaaaa acctctggtt tacatcccag tctttccagg aaccaactca gaatatgatt
    18061 cagctaaggc cttcgaaaaa gaaggtgcag aggtcaattt ggtgccattc gtgaccttga
    18121 atgaagaagc tattgtcaag tcagttgaaa ctatggttga caatatcgac aagactaata
    18181 ttctcttctt tgccggtgga ttctcggctg cggatgaacc agatggttca gctaagttta
    18241 tcgtcaatat cctgcttaat gaaaaagtgc gtgtggctat tgatagcttt atcgcccgtg
    18301 gtggcttgat tatcggtatt tgtaatggat tccaagcctt agtcaaatcg ggtctcctac
    18361 catacggaaa ctttgaagat gctaacagta ctagtccaac cctcttctac aatgatgcca
    18421 accaacacgt ggccaagatg gtggaaactc gcattgccaa taccaactca ccatggttgg
    18481 ctggtgtgca agtgggcgat atccacgcta ttcctgtttc gcacggtgaa gggaagtttg
    18541 tcgtgacggc tgaggaattt gcagagctcc gtgacaatgg acaaattttc agccaatacg
    18601 ttgactttaa cggtaaacca agtatggatt ctaagtacaa tccgaatggt tctgtccatg
    18661 ccatcgaagg aattaccagc aagaatggtc aaatcatcgg taagatgggc cactcagaac
    18721 gttatgagga tggtcttttc caaaatatcc caggcaataa agaccaacac ctgttcgcat
    18781 cagcggttaa acatttcact ggaaaataag acttacagat tttctaatag atagtatcag
    18841 taatgtaaaa gtcatgtaaa tctagctctt gatgattaca aatgaaaatt aggtataaaa
    18901 aatgacatac gaagtaaaat ctcttaatga agaatgtggt gttttcggta tttggggaca
    18961 tccagatgct gctaagttga cctattttgg actccacagt cttcaacacc gtggtcagga
    19021 gggggcagga attctctcca atgatcaagg acaactgaag cgccatcgtg acatggggct
    19081 tttatcagaa gttttcagaa atccagccaa tttggataaa ttgacaggag ctggtgcgat
    19141 tgggcatgtg cgttatgcga ctgctggcga agcttctgtt gataacatcc aacccttcct
    19201 cttccgtttt cacgatatgc agtttggctt ggctcataat ggaaatctga ccaatgcagc
    19261 ctctctcaag aaagaactgg aacaaagagg agcaattttc agcgcgactt cggactctga
    19321 aatcttggct cacctcattc gtcgcagtca taatcctagc ctgatgggca aaatcaagga
    19381 agcgctcagc cttgtcaaag gtggttttgc ctatatcttg ctgtttgagg acaagttgat
    19441 tgcggctctt gaccctaatg gctttcgtcc gctttctatc gggaaaatgg ccaacggagc
    19501 ggtggttgtt tcctctgaaa cctgtgcttt tgaggtcatt ggtgccgagt ggattcgtga
    19561 tttgaagcca ggtgagattg tgatcattga tgacgagggc attcagtatg acagctatac
    19621 agatgatacc cagttggcga tttgttctat ggagtatatc tactttgccc gccctgattc
    19681 taatatccac ggtgtcaatg tccatacggc acgtaagaga atgggagcgc aattggcgcg
    19741 agaatttaag catgaggcag atattgtagt tggtgtgccc aattcttccc taagcgcggc
    19801 tatgggattt gcggaagaat caggcttacc aaatgaaatg ggtctgatca aaaaccaata
    19861 cacccagcga acttttatcc aaccgactca agaattgcgg gagcaaggag tgcggatgaa
    19921 actgtctgct gtttcgggtg ttgtcaaagg caaacgtgtg gtcatggtgg atgattccat
    19981 tgtacgtgga acaacctctc gtcgtatcgt tcagctcttg aaagaagcgg gtgcgactga
    20041 ggttcacgtt gccattggaa gtcctgcact agcgtatcca tgtttctacg ggattgatat
    20101 ccagacccgt caggagctga ttgcagccaa tcatacggtc gaagaaactc gccaaatcat
    20161 tggtgcggac agtatgactt atctttcaat tgatggcttg attgagtcga ttggtatcga
    20221 aacagatgcg ccgaacggtg gtctctgtgt cgcttacttt gacggtgact acccaacgcc
    20281 tctctatgac tacgaagaag actatcgtag aagtttggaa gaaaagacca gtttttacaa
    20341 gtaggcgaca gattctccat taaagaaaag gaaaaaacaa atgacaaata aaaatgcata
    20401 tgcccagtcg ggtgtggatg ttgaagcggg ttatgaagtt gttgaacgga ttaaaaagca
    20461 cgtggcctgt acggagcgtg caggtgtcat gggagctctt ggtggctttg gtggtatgtt
    20521 tgacctttcc aagactgggg ttaaagaacc cgtcttgatt tcagggactg acggtgtcgg
    20581 aaccaagctc atgttggcta tcaagtacga caagcacgat accatcgggc aggactgtgt
    20641 ggccatgtgt gtcaacgaca tcattgctgc aggtgcggaa cccctctatt ttctcgacta
    20701 cgtagcgaca gggaagaatg aaccagctaa gctagaacaa gtggttgctg gtgtggcaga
    20761 aggttgtgtg caggctggtg ctgccctcat cggtggggaa acggctgaaa tgccgggcat
    20821 gtacggcgaa gacgactatg acttggctgg ttttgcggtc ggtgtggctg aaaaatctca
    20881 aatcattgac ggttcaaagg tggtagaggg agatgttctt ctcggacttg tttcaagtgg
    20941 gattcactca aatggttact ctttggttcg tcgtgtcttt gcggattaca caggtgagga
    21001 agtcctacca gaattggaag gcaagaaact taaggaagtt ctacttgagc cgactcgtat
    21061 ctatgtcaag gctgtcttgc cgctcatcaa agaagagttg gtcaacggca ttgcccacat
    21121 cacaggtggt ggctttatcg aaaatgtccc tcgtatgttt gcagatgacc tagctgctga
    21181 aattgatgaa agcaaagttc cagtgcttcc aattttcaaa gcccttgaaa aatatggtca
    21241 gattaaacac gaagaaatgt ttgaaatctt caatatgggt gtgggactta tgttggcggt
    21301 cagccctgaa aatgtagagc gtgtaaaaga attgttggat gaagcagtct atgaaattgg
    21361 tcgcatcgtc aagaaagaaa acgaaagtgt cattatcaaa tgaaaaaaat agcggttttt
    21421 gcctctggta atggctcaaa ttttcaggtg attgccgaag aatttccagt ggagtttgtc
    21481 ttttcagacc atcgtgatgc ctatgtgctt gagcgtgcaa agcagctcgg cgttctgtcc
    21541 tatgcttttg aactcaagga gtttgagagc aagacagact acgaagcagc ccttgtcgaa
    21601 ctcttggaag aacaccagat tgacttggtt tgcctagcag gctacatgaa aatcgttgga
    21661 ccaaccttat tgtcggctta tgaaggtcgg attgtcaaca ttcatccagc ctacttgcca
    21721 gaatttccag gagctcatgg gattgaggat gcttggaatg ctggcgtggg tcagtctggt
    21781 gtgaccattc actgggtgga ttcgggtgtg gatacaggcc aggtcatcaa acaggttcgt
    21841 gtgccacgac tagctgatga taccattgac agatttgaag ctcgcatcca tgaagcagag
    21901 tacaggttgt atccggaagt agtgaaggct ctatttacag attgactttt tgatgattca
    21961 tatgatatct ttgattttaa attggagtca gtgtttgttg aagacggctt caaacggagg
    22021 tatttgtaat gttagaatct aaaaaaacaa ctcgatatgt attttatgtc tatctgatgt
    22081 tattaacttg gggaatctta tttaagtttg aaacaaatcc tgaatttata gcatttttct
    22141 tagctccaag gtatatcaat tggattccat tttcagaacc actaatagtc gatggaaaaa
    22201 ttgtttttgc tgaaatgtta tttaatctga tttcctttat tccattaggt gtttgtttcc
    22261 ctttgataaa aactaattta tctagtttaa gaatagtcgg gacaggtttc ttgattagtt
    22321 tattgtttga gtgcttacag tatattttag caataggtat aacagatata acggatttga
    22381 ctttaaatac gctaggtgtc tgtgtaggct tactgattta tcaaattttt ataagagtgt
    22441 tcaaatcaca gactagaaaa tggatcaata tcttaggtat gcttagcctt ggttttgctt
    22501 atcttgtttt actgttactg catttaattg gtgtttaact aatgattaaa aaggagaata
    22561 taatgactaa acgcgtctta atcagcgtct cagacaaagc gggcattgtt gaatttgccc
    22621 aagaactaaa aaaacttggt tgggagatta tctcgacagg tggaactaag gttgcccttg
    22681 ataatgctgg ggtggagacc attgcgatcg atgatgtgac gggcttccca gagatgatgg
    22741 atggccgtgt taagactctt catccaaata tccacggggg tcttctcgct cgtcgtgatt
    22801 tggatagcca cttggaagca gccaatgaga accaaattga gcttattgac cttgtagtgg
    22861 tcaaccttta tcctttcaag gaaacgattc tcaaaccaga cgtgacgtac gctgatgctg
    22921 tggagaatat tgatattggt gggccatcta tgcttcgttc agcagcgaag aatcatgcca
    22981 gcgtgacggt tgtggtagac cctgctgact atacagtggt tttggacgag ttgtcagcca
    23041 atggtgaaac gacttacgaa acgcgtcaac gtttggcagc caaggttttc cgtcacacag
    23101 cggcttatga cgctttgatt gcagaatact tcacagctca agtgggcgaa agcaaacctg
    23161 aaaagttgac cttgacctat gacctaaaac aagctatgcg ttatggggaa aatccccagc
    23221 aggatgcgga cttctaccag aaagctttgc cgactgatta ctccattgca tcagccaaac
    23281 agcttaacgg taaggaattg tcattcaaca atatccgcga tgcagatgct gccattcgta
    23341 ttatccgtga cttcaaagac cgtccaaccg ttgtggctct caagcatatg aatccatgtg
    23401 gtatcggcca ggcggatgac atcgagacgg cttgggatta tgcttatgag tctgacccag
    23461 tgtctatctt tggtggtatc gctgttctta accgtgaggt ggatgctgcg acagctgaga
    23521 agatgcacgg cgttttcctc gaaatcatca ttgcaccaag ctatacggat gaagcgctag
    23581 ccattttgat caataaaaag aaaaacttgc gtatccttgc cttgccattt aatgctcaag
    23641 aggctagcga agtggaagca gaatacacag gtgtagtcgg tggacttctc gtgcaaaatc
    23701 aagacgtggt caaggaaagc ccagctgact ggcaagtggt gaccaaacgt cagccaaccg
    23761 agacagaagc gactgctctt gagttcgctt ggaaggctat caagtacgtc aaatcaaatg
    23821 gtattatcgt gaccaacgac cacatgacac ttggtgttgg tccaggtcaa accaaccgtg
    23881 tggcttctgt tcgccttgcc attgaccaag ccaaagatcg tctggacggg gcggtccttg
    23941 cttcagatgc cttcttccca tttgcggata acgtggaaga aatcgccaaa gcaggaatta
    24001 aggccatcat ccagcccggt ggctctgtcc gtgaccaaga atccatcgaa gcagcggata
    24061 aatacggctt gactatggtc tttacaggtg tgagacattt tagacattaa gaagataaaa
    24121 gggaagaaaa cagtttcttt ccttttttgg cttaaaatgc taactgaaac aagattaaaa
    24181 cgaacttttt tgatataatg ttggtaaata attcgcaaaa gaggttgagg aatgaaactg
    24241 cttgttgtcg gttctggtgg tcgtgagcat gcgattgcta aaaagttact tgaatcaaaa
    24301 gacgtggaaa aagtctttgt agctcctggg aatgatggga tgactctgga tggtttggaa
    24361 ttggtaaata tctctatttc cgaacattct aaattgattg acttcgcaaa gaccaatgat
    24421 gttgcttgga cctttatcgg tccagatgat gcccttgctg ctggtatcgt ggatgatttt
    24481 aaccaagctg gacttaaggc ctttggtccg actagggctg cagcggagct ggagtggtcc
    24541 aaggatttcg ccaaggaaat catggtcaaa tacggcgttc cgacagcaac atatggcaca
    24601 ttttcagatt tcgaggaagc caaggcctat atcgaaaagc atggtgcgcc tatcgtagtc
    24661 aaggcggatg gcttggcact tgggaagggt gtcgtcgttg ctgagacggt tgagcaagcg
    24721 gtcgaagccg ctcatgagat gcttttggac aataaatttg gtgactcagg tgcgcgcgtg
    24781 gttattgagg aattccttga aggagaggaa ttttcactct ttgcctttgt caatggtgat
    24841 aagttctaca tcatgccaac ggctcaggac cacaaacgtg cctatgatgg cgacaaaggg
    24901 cctaacacgg gtggtatggg tgcctatgcg ccagtcccac acttaccaca gagtgtagtt
    24961 gatacagcgg ttgacaccat tgtcaagcca gttctagaag gggtgattaa agaaggtcgc
    25021 ccttatctgg gagttcttta cgcagggctt atcctgacag ctgatggacc gaaagtcatt
    25081 gagttcaacg ctcggttcgg agatccagaa actcagatta tcttgcctcg cttgacctct
    25141 gactttgctc aaaatatcac agatatcctg gatagcaagg agccaaatat catgtggacg
    25201 gacaagggtg tgactctggg tgtggttgtc gcatccaagg gctacccgct agactatgaa
    25261 aggggcgttg agttgccagc aaagacagaa ggcgatgtca tcacctacta tgcaggggct
    25321 aagtttgcgg aaaatagcag agcactgctc tcaaacggcg gacgagttta tatgctcgtt
    25381 accacagcag ataccgtcaa agaagcccaa gccagcatct accaagaact atcccaacaa
    25441 aaaatagaag gactcttcta ccgaacagat atcggaagca aggcaattaa gtaaagatat
    25501 aagaataacg cgccgtagtc gccaaacacg ataatggtcg tcgtggtgaa aagaccagaa
    25561 cagtgaatgt tctggtcagg gggaaacttg gagaccttag gctcaaagtt taggaatgaa
    25621 accgaaggtt tgcttccgcc tccatcacct aagaccatta tcaaaaagaa aaataaaaat
    25681 tcacaaaata cgttaatgat cgtatggttt gcgagcgtta gcgagctaat atagaacaat
    25741 caccgccgtt gtgaaagaac gattggatga taatccaatc gttcagggaa attggaagac
    25801 cttgggtttc caatttaggc atgagacacc tttggtggct gctgccgtcc ctcacaagct
    25861 aaggtgattg ttgaaaaaga ggaaaaagga gaagaaatga aaccagtaat ttccatcatc
    25921 atgggctcaa aatccgactg ggcaaccatg caaaaaacag cagaagtcct agaccgcttc
    25981 ggtgtagcct acgaaaagaa agttgtttcc gcacaccgta caccagacct catgttcaaa
    26041 catgcagaag aagcccgtag tcgtggcatc aagatcatca tcgcaggtgc tggtggcgca
    26101 gcgcatttgc caggcatggt agctgccaaa acaacccttc cagtcattgg tgtgccagtc
    26161 aagtctcgtg ctcttagtgg agtggattca ctctattcta tcgttcagat gccgggtggg
    26221 gtgcctgttg cgaccatggc tatcggtgaa gctggagcga ctaacgcagc tctctttgcc
    26281 ctccgtctcc tctctgtaga agataagtcc attgcggatg cacttgccaa ctttgctgaa
    26341 gaacaaggaa aaatcgcaga ggagtcgtca aatgagctca tctaaaacaa tcggaattat
    26401 cggtggcggt caactgggtc agatgatggc catttctgct atctacatgg gccacaaggt
    26461 tatcgcgctg gatcctgcgg cggattgccc ggcctctcgc gtggcggaaa tcattgtggc
    26521 accttataac gatgtggacg ccctccgtca gttggcagac cgttgcgatg tcctcactta
    26581 cgagtttgaa aatgtcgacg ctgacggttt ggatgccgtt atcaaggatg gacaactccc
    26641 tcaaggaaca gatctgctcc gcatttcgca aaatcgtatt tttgaaaagg actttttgtc
    26701 aaacaaggct caagtcactg tggcacccta caaggtcgtg acttctagcc tagacttggc
    26761 agatatcgac ttgtcgaaaa actatgtcct caaaactgcg actggtggct acgatggtca
    26821 tggacaaaag gttattcgtt cagaagcaga cttggaagca gcctatgcgc tagcagactc
    26881 agcagactgc gtcttggaag aatttgtcaa ctttgacctt gagatttctg tcatcgtgtc
    26941 aggaaatggc aaggaggtga cgtttttccc agttcaggaa aatatccacc gcaacaatat
    27001 cctgtctaag accatcgtac cagcccgcat ttctgaaagt ctagcagaca aggctaaagc
    27061 tatggcagtg cgaatcgcag aacaactcaa cttgtctgga actctctgtg tggaaatgtt
    27121 tgcgactgat gatgacgtta ttgtcaatga aatcgcccca cgaccacata actctgggca
    27181 ctattctatt gaagcctgtg atttctctca gtttgacacc catattctcg gtgttctcgg
    27241 agaaccatta ccagtcatca aactccatgc gccagccgtt atgcttaatg tcctcggtca
    27301 gcatgtcgag gctgctgagc aatatgtgac agaaaattca agcgcccacc tccacatgta
    27361 tggtaaaata gaagcgaagc acaaccgcaa gatgggacat gtgaccttgt ttagtgatgt
    27421 accggatagt gtggatgagc ttggggaagg gattgatttt taggacaagt ctatgataca
    27481 aattatcgtt aatacattta ttgaaaagga taagactgga gcagttgttg aagtgttgta
    27541 tgccagtgct gaccaagata aggtacaagc taaatatgaa gaactagctg cacaataccc
    27601 cgaaaattat ttagctatct atgatgtacc gctggatacg gatttgaata cactagatca
    27661 ttacccgtct gtgtttattg aaaaagagga gtttgagtag aaatcttggt ttacctagat
    27721 agcttattcc caacagctta agaagaaagg aaaaattaac acatgatcaa ccgttactct
    27781 cgccctgaga tggcgaatat ttggagtgaa gaaaataaat accgtgcttg gcttgaggtg
    27841 gaaatcctct ctgacgaggc atgggctgag ttgggggaaa tccctaagga agatgtggct
    27901 ttgattcgca agaaggcgga ctttgacatc gaccgtattt tggaaattga gcaggagacg
    27961 cgccacgatg tggtggcttt cacgcgtgcg gtttctgaga ctcttggtga agagcgcaag
    28021 tgggttcact atgggttaac ttctaccgac gtggtggata ctgcttatgg ttacctctac
    28081 aagcaggcca acgacatcat ccgtcgtgac cttgaaaact tcactaatat catcgctgac
    28141 aaggccaagg agcacaagtt caccatcatg atggggcgta ctcatggtgt gcacgctgag
    28201 ccgacaactt ttggtcttaa attggcaact tggtatagcg aaatgaaacg caatatcgag
    28261 cgtttcgagc atgcggctgc tggtgtagaa gctggtaaga tttctggtgc ggttgggaac
    28321 tttgccaata tcccaccatt tgtagaggag tatgtctgca acaagcttgg tatccgtgct
    28381 caagaaatct ctacacaagt ccttcctcgt gaccttcacg ctgagtactt tgcagttctt
    28441 gccagcattg cgacttcaat cgaacgtatg gcgactgaga ttcgtggtct acaaaaatct
    28501 gagcaacgcg aagtagaaga gttctttgct aaagggcaaa aagggtcttc agcaatgcct
    28561 cacaaacgca acccaatcgg ttctgaaaat atgactggtc tggcgcgtgt cattcgtggt
    28621 cacatgatta cggcttatga aaacgtcgct ctctggcacg aacgcgatat ttcccactca
    28681 tcagctgagc gtatcatcac accagatacg accattttga ttgactacat gctcaaccgt
    28741 tttggaaata tcgtcaagaa cttgacagtc ttcccagaaa atatgatccg aaacatgaac
    28801 tcgacttttg gtcttatctt tagccaacgg gctatgttga cattgattga aaaaggcatg
    28861 acccgtgagc aagcctatga cttggtgcaa ccaaaaacag cctactcttg ggacaaccaa
    28921 gtagacttta aaccacttct tgaggcagat tcagaagtaa catcacgtct cactcaagaa
    28981 gaaatcgatg aaatcttcaa cccagtttat tacaccaaac gagtggatga tatctttgaa
    29041 cgtcttggac taggtgatta attaaaaaat aaacagcgag cttcaatctc gctgtttatt
    29101 ttttatcgaa aagacttagt cttcttttct tttagtgagt ccataggctg ctagtgtgga
    29161 catgagtcct gcgactacta gttctgcaga atcgtgagtt cctgtttcag gaagtttttt
    29221 ctctgttacc acaggagctg gatcttgagg aagagctttg ctttcctcag caggagcagt
    29281 tgatggagct ggttggcttg ggatttctag ttttggtttt tcttcagcaa tagcggcttg
    29341 tccgttttca tcgcctacat gtgttaccat agttccgact tcgactattt gagtaacggc
    29401 ttcctgtgct acgacactat ttacaagtgt tttcacttcc ttaccatcgg cagaagtgct
    29461 cacagagtag aagttgctac gatgtccatt gacgccctta gtaatgactt gtgtttttcc
    29521 tttgagtaag agtggatttt cacgagtcac tgtggtaaat ggaatttctt ctttttggat
    29581 atccagtcta ggttttacct cagtagttgg tgcaagacca ctttcatcac ccttgtgagt
    29641 tacaggagcg ccaacttcaa ccacttggtt tataacttct ttggttacct ggctatcaag
    29701 gactgtttct gttgtttttc cattttcagt gagtacagag atgtaatgag ttcgttcacc
    29761 tttgactcct gctgtgataa tattttcctg accagctggg aggttaggat tttctttctt
    29821 gataacttca aatggaattt cttcagttct tgtgatgagt tctggtctgg tttcaacatt
    29881 ggcagccact tcattttcat ccaggcttcc tgaatgagtt gcagctggtt tgaggccttg
    29941 aagagcggct tttaggttgg ctacaagtgt gtcaagctca gcttgtttat tacggttgag
    30001 gttgtaattt agagctgttt tagctgcgtc aagggcctca agactttctt tactatatcc
    30061 ttctaagttt gtaggaattt tagctaattc ttcgcggaga gcattataat tagcacggaa
    30121 gtagtctttg ttgtggtctg caaaggcagt catgagttca aagatttcct cttccttgta
    30181 ctcagcgctt ggtctatctg cccagattga aagcatactt ccgactgttg gaagatctac
    30241 ttcaggatat ttggtagaag ctagttgatt gaatggtgtt tttccagtat tctcaatagc
    30301 tttcttgagg aaaccaccac catcttctgg tttttgacca agaatgtagt accagtcacc
    30361 gttggtattc aagaatttat agcctttgct tgctaggtat tgaggtgatg cgaggttata
    30421 tccccaccag cctttagacc agtaagaaat caagacatct ttgtcaaact gaacatcgtc
    30481 cttgtcttca tagtagaagc catcgttgaa ggccattggt tgaagccctc tttctttggc
    30541 catagctgcg agggtgttgg catattcggc aaatttgcca tagagttgat accacttgag
    30601 gtagtaccag ccttgggcac tagtcgcatc gttggcgtat tcgtcagtac caaagttgaa
    30661 aatctttgtt ttacctgcaa agaagtccat gtatttaccg atgagggctt ttacaaagtt
    30721 catcgcttct tcgtttttca agtccatagt tgtttttgaa actttatcaa agtgggcttg
    30781 aggattttta atacctaatt tttccatggc aaccagcata gcatccatgt gacctggact
    30841 gttaatagct gggatgagac cgatgtcctt agatttagcg tattcaatta gctctgttac
    30901 ttctgcctgt gttagtgcag taccgtttgg atcgtcgtag taagctttag ttccttcgat
    30961 aatagctttt ttaacgtcat cactagcata ggtttttccg ttggcagtaa tggtcatatc
    31021 atcgagtaga aagcgaagtc cgtcatttcc tagaaggaga tggacatcag aatatccgag
    31081 ctcactggcc ttgtctacga tgcgtttgag ctggttcaga gtaaagtatt tgcgtccagc
    31141 atcgattgag attaccttgt ttttggcaag tttttcaacc tcacgtttag cttcttcttc
    31201 ttttagagct tcaggcgtga gggtcaagtt gttgacagtt tcttgaagtt tagcaatggc
    31261 ttgatcaatc gtatcttgtt gggcacggct aaggttgcta tcgagagagc gaatagcttt
    31321 ttcagcttct tttacggccg tgacgctttc tgcagtataa cggttcaggt cttttggtac
    31381 ctcgttaagt gcttgctctg cagattcata atcagctgcg aagtattcag cgttggcatt
    31441 tgcaaaatga cgcatgagtt tgaagaggcg tgatggtgaa taacgtgcag atggagtgtc
    31501 agcccaagca gctaccatac caccgatgat tgggatatca gctccttctg tttttggtac
    31561 agaagtgatt ggtgtgtttt taataccatt gagcccctga tcgagattgt accagccttg
    31621 gccatcagcg tttcgtccaa gaacgtagta ccaagcatca ttggtattaa ggatttggtg
    31681 acctttttca gctagtagtt tagaagaagc gacatcgtag cctccccaac caccagtcca
    31741 catagaaacg atgatgtctt tgtcaaaact accaaagctt gtgtcgctat tgtagtagat
    31801 accgtcgtta aaagccattg gtttgagacc gtgcgatttt acaatacgag cgaggtcatt
    31861 ggcgtaggca ataaattttt catagccttt tacagggtag ccttcgtttg gatagtattt
    31921 atcagcttga agcacactcc aacctttagc atctgtcgca tcattggcat attcatcaag
    31981 tccgatgttg aagatttcag tctttttcgc gaaataagca gcatacttgt cgataagggc
    32041 ttttgtaaaa gcgacagctt gttcgttgtc aagatcgaca gtacgggctg atttcttccc
    32101 aaaatagcta aagttagggt tttggattcc caattctttc atggcattga gaatcgcatc
    32161 catgtgtcca ggactattta ctgtcggaat gagaccgata cctttatctt tggcatagtt
    32221 aatcagatct gtcatttgac tttctgttaa gtgattgccg tttggatcgt tgtaataatc
    32281 atttgtacct ttttcaatgg cgcgtttgac atcgtcactg gcataggtct tgccgttagc
    32341 tgtgatgctc atatcgtcca acatgaaacg gagtccatca tttccgacta ataggtgtaa
    32401 atcagtgtag ccataatgtt tcgctttatc gatgatttcc ttgagttgtt ctggtgagaa
    32461 atatttacgt ccagcatcaa tagaaacaat tttctttttc gctagttttt catttacagt
    32521 tgcagcacgt tcctttcctg cctctgtttc cggtttgtca gcctctgctt tcgcttcatc
    32581 ttttttagct ggtttatcct tgtcagtctt gtctgtattt gactctttag aatcaacctc
    32641 tttcgcttct tcctttttag ggctagcttc ttctgccttt ttattagcag tttctttttc
    32701 agcagaagtt ggagttacca cttctgcttt atcactagga gttgaactaa cttcctcttg
    32761 tggtttttct tctgtttttg gaagactagc taccttatca gtagctggag tttctgtttc
    32821 tacagttttt ggagcttctg gttgaagcac tgctttaggt gtttcctcag tccgattttc
    32881 ggatgattga ggggaatcag aaaccgtatg gatggtcggt tggttttctg tagtagtagg
    32941 agtaactcca tcggctgcaa cagtctgtgc ttggaaggca aatccaatta gaacagaagc
    33001 tgctcctaca gcgtatttac gaatagaaaa acgctgttgt ttttcatgtt tcattgccaa
    33061 acctcctgat tgcattgtta tattgatagc gattatataa atcaacgcct ttattttatt
    33121 tcttatatta acgagagtca agaggagatg acaaaaaact ataataagta taaaaaaata
    33181 taaaatttaa acttaagatt tcagattggt cggaaaaaat acgtatatat atctagtata
    33241 atttttggtt ctatttctat aaaatattcc acaaattata gaatcttcca aaaataggta
    33301 agcgctacct ttttggtgtg gtataataag catagaaaaa gcccaagcga ttagctcagg
    33361 ttttcttctt agtgatcacg gttacatgag ataaatttaa tcttgtagta atcagatcgt
    33421 ttgtaagttt cactgtattc taaaacttgg ccagttgatt cgagtttggt gattttagtt
    33481 tgtaggacag tagggaattg ttcatcgact ccgaggactg aagccgcatg ttctggagtt
    33541 ggaaagacta tttcgttgat ttcttcaaag tgttcatcat tcatgtgaat gtggtagtct
    33601 aacttgaaac gattatagat agaactatag tattcaaggt ttggataatt tgcgttgata
    33661 tattgttctg ggatgtagga tgtatggtag atataaacga caccgtttga ttcgcggata
    33721 cgttcaatct tgtagtagaa ttgatcgcca cgtagaccca atttttccaa gtaaacaagc
    33781 ttgtttccgc gttcaattga aagaacagtt accttatcat ctttagcatt gaagagttca
    33841 atatctgaaa actctacaag cttgtgtttg cgtgcacgtg aaacgaaggt tccttttcct
    33901 tgttggcgga caatatagcc atctttggca aggtcgttta aggcgcgaac aactgtgata
    33961 gagctgacat cgtacattga aatgagttct gcttcagtgt aaaatttatc tccactgcta
    34021 aactgcccag agatgatttt attttttaat tcgtctttta tgtattgata tttgggaatt
    34081 gccatatttt tacctctatt ttccttctcc atagaaaatt ttaccataaa agcgaaaaaa
    34141 atagtagtct tttgaataaa aaattagaat aatagtttga aaaattaagc gatataggga
    34201 ttgctcatat tttataaatg cattctatac aaaattattt taggtactat attccaaatt
    34261 taaagagcgt ttgaaatggg tctaaaaatg tttgaaacta attgaatgtg tgtataatag
    34321 taaaaatgca gtaaatatcc cgacaatttc tattgacaat tcaaacagat tggtttataa
    34381 ttaatataac aacaaatgaa agcgcaaact ttcgcggtcg gaaggtagtt ttatgacacg
    34441 atttgagata cgagatgatt tctatctcga tggaaaatca tttaagattt tatctggtgc
    34501 cattcattat tttagggttc ctccagagga ttggtatcat tcgctctata acttgaaggc
    34561 tcttggtttt aatacggtag agacttatgt tgcttggaat ttacacgagc ctcgtgaagg
    34621 tgaatttcat tttgaaggtg atctggattt agataaattt ctccaaatag cgcaggattt
    34681 gggtctctac gcaattgtgc gtccgtctcc atttatctgt gcggaatggg aattcggtgg
    34741 cttaccagct tggctcttga ccaagaacat gcgaattcgc tcatccgacc cagcatatat
    34801 cgaggcagtt ggtcgctact atgatcagtt attgccaaga ctggtgcctc gtttgttgga
    34861 caatggtggc aatattctca tgatgcaggt tgaaaatgag tatggttctt acggagaaga
    34921 taaggcttac ctgagagcga ttcgacagct aatggaagag cgtggcgtaa cctgtcctct
    34981 ctttacatca gatggtccat ggcgagctac tctgaaagct ggaaccttaa ttgaagagga
    35041 cctctttgta acaggaaact ttggttctaa ggcaccttac aacttttcgc agatgcagga
    35101 attctttgat gaacatggta agaaatggcc actcatgtgt atggagttct gggatggttg
    35161 gttcaatcgc tggaaagaac cgattatcac acgggatcct aaggaattgg cagatgcagt
    35221 tcgagaggtt ttggaacaag gctctatcaa tctttacatg ttccacggtg gtacaaactt
    35281 tggtttcatg aatggttgct cagctcgagg aactttggac ctgccacaag ttacgtctta
    35341 tgattacgat gcccttctgg atgaagaagg aaatccaact gctaaatatc ttgcagtcaa
    35401 gaagatgatg gcaacacatt tttcagagta tccgcagttg gaaccactct acaaagagag
    35461 tatggagttg gatgctattc cactagttga aaaagtttct ttgtttgaaa ccttagatag
    35521 cttgtcaagt cctgtagaaa gtctctatcc tcaaaagatg gaggagctgg gacaaagtta
    35581 tggctaccta ctttatcgaa cagaaacaaa ctgggatgca gaagaagaaa gacttcgtat
    35641 cattgatggt cgagataggg cccagctgta tgtcgatggt cagtgggtta aaactcaata
    35701 tcagacagag attggggaag atatttttta tcaaggtaaa aagaaagggc tatctaggtt
    35761 agatatcttg atagaaaata tggggcgtgt caactatggg cataagttct tagcggatac
    35821 gcaacgtaag ggaattcgga caggggtctg taaggatctg catttcttac taaactggaa
    35881 acactatcca ctcccactag acaatcctga gaaaattgat ttttcaaaag gatggactca
    35941 aggacaacca gccttttacg cttatgactt tacagtcgaa gagccaaaag atacttacct
    36001 agacttgtct gagtttggta agggagttgc ctttgtcaat gggcagaatc taggacgttt
    36061 ttggaacgtt ggcccaactc tctcacttta tatccctcat agctatctca aggaaggtgc
    36121 caaccgtatc attatctttg aaacagaagg tcaatataaa gaagagattc atttaactcg
    36181 taaacctaca ctaaaacata taaaggggga aaacttatga caattgtagg atgccgtatt
    36241 gatggacgtt tgatccacgg acaagtagcc aatctttggg ctggaaaact aaatgtttca
    36301 cgcattatgg ttgtagacga cgaagttgtc aacaacgata ttgaaaagag tggtttgaaa
    36361 cttgcgacac caccaggtgt gaaattgagt attttgccag ttgagaaagc tgcagccaat
    36421 attcttgctg gtaaatacga tagccaacgt ctctttatcg tggctcgtaa accagaccgc
    36481 ttccttggtt tggtagaagc aggtgtacca cttgaaaccc ttaatgttgg gaatatgtct
    36541 caaacaccag aaactcgttc tattacacgt tctatcaacg tagtagacaa ggatgtggaa
    36601 gacttccaca aactggcaga aaaaggtgtt aaacttactg ctcagatggt tccaaatgat
    36661 ccaatttcag actttttgag cttattaaaa taggaaaaaa atttttagga ggtcattgtt
    36721 atgatacaat ggtggcaaat tttacttctc actttgtact cagcttatca aatctgtgat
    36781 gagttgacga tcgtttcatc tgcaggttcc cctgtatttg ctggtttcat tactggttta
    36841 atcatgggag atgtgactac tggtttactt atcggtggta acttgcaact gttcgttctt
    36901 ggggttggta ccttcggtgg tgcttctcgt atcgacgcaa cttctggtgc ggttcttgcg
    36961 acagccttct ctgtttcaca aggaattgat gcaccgcttg ccattactac aatcgctgta
    37021 ccagtagcag ctctcttgac ttacttcgac gttcttggtc gtatgactac taccttcttc
    37081 gctcaccgtg tggatgctgc aatcgaacgc tttgactata aaggtattga acgcaactac
    37141 ttgcttggtg cgattccgtg ggctctatct cgtgcccttc cagtcttctt tgcccttgct
    37201 tttggtggtg cctttgtaca atcagtagta gacttcgttg aagcctacaa atgggttgca
    37261 gatggcttga cactcgcagg acgtatgctt ccaggtcttg gatttgcaat cttgcttcgt
    37321 taccttccag ttaaacgtaa ccttcactac cttgctatgg gatttggttt gacagctatg
    37381 ttgactgttc tttactcata tgtaacaggt cttggtggcg ctgttgctgg tatcgtaggt
    37441 actcttcctg ctgaagttgc tgaaaaaatt ggtttcgtga acaacttcaa aggtttgtct
    37501 atgattggta tttctatcgt aggtattttc cttgcagtgc ttcacttcaa aaatagccaa
    37561 aaagtagctg tagcagcacc ttctacacca tcagaaagtg gggaaatcga agatgacgaa
    37621 ttctaattac aaacttacaa aagaagattt taatcaaatc aacaaacgta gcttgtttac
    37681 tttccaatta ggttggaact acgaacgtat gcaagcttct ggttaccttt acatgatctt
    37741 acctcagttg cgtaaaatgt atggtgatgg aactcctgaa ttgaaagaaa tgatgaaagt
    37801 tcatactcaa ttcttcaata cttcgccatt cttccatacc attatcgctg gttttgacct
    37861 tgccatggaa gaaaaagatg gtgtaggttc aaaagacgcc gttaacggta tcaagacagg
    37921 tttgatggga ccattcgctc ctcttgggga tacaatcttt ggttcacttg tacctgctat
    37981 catggggtca gtcgcagcaa ctatggctat cgctggccaa ccttggggga tcttcctttg
    38041 gattgcagtt gcagtagcgt atgacatctt ccgttggaaa cagttggaat ttgcttacaa
    38101 agaaggggtt aaccttatca acaacatgca aagtaccttg acagctttga ttgacgctgc
    38161 atctgtactt ggtgtcttca tgatgggtgc tcttgtagca acagtgatta actttgaaat
    38221 ttcttacaag ttgccaatcg gtgaaaagat gattgatttc caagacatct tgaaccaaat
    38281 cttcccacgt ttgcttccag caatctttac tgcctttatc ttctggttgc ttggtaagaa
    38341 aggtatgaac tctactaaag ctatcggtat tattatcgta cttgctttgg ctctttctgc
    38401 ccttggtcac tttgcacttg gaatgtaatt ccttatgact aaatcattaa ttttggtgag
    38461 ccatggtcgc ttctgtgagg agcttagggg tagcacagaa atgattatgg gtccacaaga
    38521 caacatttac acagtagctc ttcttccaga agatggccca gaagaattta ctgctaaatt
    38581 tgaagctgtt attgaagggt tggatgattt cctagtcttt gcggatcttc tcggtgggac
    38641 accttgtaat gtggtgagtc gcttgatcat ggaaggtcgt gatattgacc tttacgcagg
    38701 gatgaatctt ccaatggtga ttgaatttat caatgcgagc cttacaggcg cagatgcgga
    38761 ctacaagagc cgtgctgcag aaagtattgt gaaagttaat gacctgttag cgggcttcga
    38821 tgatgacgaa gatgaataat actcttcgaa aatctcttca aactacgtca atgtcgcctt
    38881 gccgtaggta tatgttactg acttcgtcag tcttatccgg caacctcaaa acggtgtttt
    38941 gagctgactt cgtcagttct atctgcaacc tcaaagcagt gctttgagca gcctgcggct
    39001 agtttcctac agattttagt tggaactcga ttcaattcat gtgacaacgt gaaaatcgtt
    39061 agagcatttt atatagaata tacatgggaa tggagcttac tcccattccc atatttaata
    39121 gaaaaagagg aactcaatgc tacattatac aaaagaagac ttgctcgaat tgggtgcaga
    39181 aatcactacg cgtgaaatct accaacagcc tgatgtatgg agagaagctt ttgaatttta
    39241 tcaagcaaaa cgtgaagaaa ttgcagcctt tctacaagaa atcgctgata aacatgacta
    39301 tattaaggtt atcttgacag gtgctgggac ttctgcttat gtgggagaca ccttgctacc
    39361 ttactttaag gaagtctatg acgaacgcaa atggaatttc aatgctattg cgacaacaga
    39421 tatcgttgcc aatccagtaa cctatttgaa aaaagatgtg gcaactgtcc ttgtgtcttt
    39481 tgctcgtagt gggaactcgc ctgaaagtgt ggcgactgtt gatttggcca aatccttggt
    39541 ggatgagctt tatcaagtga cgattacttg tgcagcagat ggtaaattgg ctcttcaagc
    39601 tcacggtgat gaccgtaatc tcttgctctt gcaaccagct gtctctaatg atgctggatt
    39661 tgccatgact tctagcttta cgtctatgat gttgacagct ctcttggtct ttgatcctac
    39721 agaatttgct gttaagtctg aacgttttga agttgtatct agtcttgccc gtaaagtttt
    39781 agacaaggca gaagatgtca aagagctcgt tgatttagac tttaaccgtg tcatctatct
    39841 aggcgctggt cctttctttg gacttgctca tgaagctcag ctcaagattt tggaattaac
    39901 tgctggtcaa gttgcgacca tgtatgaaag cccagttggc ttccgtcacg gtccaaaatc
    39961 tcttatcaac gacaatacag ttgttttggt ctttggtaca acgacagact acactcgtaa
    40021 gtacgacttg gacttggttc gtgaagttgc tggtgaccag attgctcgtc gtgttgtgct
    40081 tttgagtgat caagcttttg gtcttgaaaa tgtcaaagaa gtggcccttg gttgtggcgg
    40141 tgtcttgaat gatatttacc gtgtcttccc ttacatcgtt tatgcccaac tctttgctct
    40201 attgacttca ctcaaggtag aaaataaacc agatacaccg tctcctacag gtacagtaaa
    40261 ccgtgtagta caaggtgtca taattcacga atatcaaaag taagacagcg tttatgaatt
    40321 cttgacaaga ggatttgtaa attatcagat aaaccataga ttgtcagtac gctttctatg
    40381 gtttgtttgc ttgagagaaa tagtaaaagg agaacagaat gaaagcatac acagagcgtg
    40441 tatttggaaa tgttgagggc gaggatgtct tggcctatcg atttgagaca gacggtggct
    40501 accaacttga ggttatgact tatggcgcga ccatcttgcg ctatgtcgca cctgacaagg
    40561 ctggaaattt tgccaatgtt atcttgggat ttgatgactt tgatagttat gtaggcaata
    40621 gtcccaagca tggagcaagt gtaggtcctg tagcgggtcg tattgcaggt gcgacctttg
    40681 agctcaatgg taagacctat gaccttgagg ttaataatgc tagcaactgt aatcacagtg
    40741 gttcaactgg ttgggattcc agcttgtttg aagttgaaga agtaagcgat catggcttga
    40801 ctctctacac agagcgtaca gatgggacag gagggttccc tggaaatctc aagatttgga
    40861 tcagttatca cttggaagaa actggtgcct atgaaatcag ctacaaggta acgaccgatc
    40921 aggatacgct ggtcaatcca accaaccaca gctatttcaa cttgtctggt gatttcacgc
    40981 agacgattga ccgtcatgtc ttccaactaa acacagaggg catttactca atcgctcctg
    41041 acggtgttcc tgccaaaact ccagaagcca accgtgatgt ggtcaaacac gtctacaatg
    41101 gtaccttgtt gaaggatatc tttgcagaag aagatgagca aatccagctg gcatcaggtt
    41161 tggatcatcc atttgccctt cctgcaggcc atgacaatgc tggattcctt tatgaccaaa
    41221 attcaggtcg cttcctgctt ttcaagacag aagctccttg ctttgtggtc tacacagcaa
    41281 actttgtgga tgaaagtgtc atcataggag gtcagccaat gctacagcac aatgggattg
    41341 ctcttgaagc gcaagcttta ccagatgcca ttcacagtga ccttaaaggc caagtcattc
    41401 ttaaagctgg tcaaaccttc accagtaaga cacgttatga acttgttgtg aagtaaaaga
    41461 gtcattgcgc ctacttttgg gagctaggaa taggtacgca gagacaaata gtaggaaaat
    41521 atgatataac taagcgttga aagctatctg ttaatataat attcaaacta caataaggag
    41581 taagaaagaa acgaagaaaa ttgtatttgc tagtgccttg gctttgacct tggctggagc
    41641 agttttgaca aatgatgttt ttgcgaacga cagacttgtg gcaacacaaa ctactgatgg
    41701 taaaaatgaa aatgtattga cctcagaggt gctaaaacct tctagtggca atgttttggt
    41761 tggaatcaaa ggagaatttg tggctcctca tcaacaatct attttggatg ccatcaatgc
    41821 tatctgtaaa gaagcggctg acgaaggttt ggtagataag tatgtcccta tcaaatggtc
    41881 aactgaccta gaaaaggcag cttttgccag agctacagaa gcatctataa ccatggatca
    41941 tacccgtctt tctagcaaag atctttggag tgcctttcca acttctaata gtataatggg
    42001 agaaaatttg gcatggaatc atgacggttt tctaaaagct attgaacaat ggcgtgctga
    42061 aaaagcagat tatgtggaga aaaaaatagt ggttcagaca acgggaaatc tggtcactat
    42121 gagtcgctaa ttaaccctaa atttacacac atggggatgg cagcttttaa aaatcctaac
    42181 aatcaataca aagctattac aattgctcaa actctaggtg atgatgcttc ttcagaggaa
    42241 ttggctggta gatatggttc tgctgttcag tgtacagaag tgactgcctc aaacctttca
    42301 acagttaaaa ctaaagctac ggttgtagaa aaaccactga aagattttag agcgtctacg
    42361 tctgatcagt ctggttgggt ggaatctaat ggtaaatggt atttctatga gtctggtgat
    42421 gtgaagacag gttgggtgaa aacagatggt aaatggtact atttgaatga cttaggtgtc
    42481 atgcagactg gatttgtaaa attttctggt agctggtatt acttgagcaa ttcaggtgct
    42541 atgtttacag gctggggaac agatggtagc agatggttct actttgacgg ctcaggagct
    42601 atgaagacag gctggtacaa ggaaaatggc acttggtatt accttgacga agcaggtatc
    42661 atgaagacag gttggtttaa agtcggacca cactggtact atgcctacgg ttcaggagct
    42721 ttggctgtga gcacaacaac accagatggt taccgtgtaa atggtaatgg tgaatgggta
    42781 aactaggctc aggccatagg taaagcattc atcttactta gcaaaaagaa tgaacgataa
    42841 gaaagaggtt gatggcgaac attggcctct tttgatttat aaagattgga ttcttgtcgc
    42901 ctcaatttca gacttttcta ttgtaagcta atattttata gcccattaaa agcataagcg
    42961 gtaatctaat ttaaaaaatg ctgtaattag tctgaagtcc acacttactt gttgagatgt
    43021 tatctctgtt ttttatcgtt ataatttact gtatttttta tagtatgcag aatattttta
    43081 agtatatttc aatagaaatt tctatcgatt tattgtataa tgataagtaa ttgttgaaaa
    43141 gtactcagaa aattccatac tatattattt ttatgtttat acttttatgc tataaaatat
    43201 agattgatat aaagaatata gaaaaagcga ggttaatatg agccgaaaaa gcattggtga
    43261 gaaacgccat agtttctcga tgagaaagtt gtcagtggga ttggtatcag ttactgtatc
    43321 tagtttcttt ttgatgagtc aagggattca atcggtatcg gccgataata tggaaagtcc
    43381 aattcattat aagtatatga ccgagggtaa attgacagac gaggaaaaat ccttgctggt
    43441 agaggccctt ccacaactgg ctgaagaatc agatgatact tattacttgg tttatagatc
    43501 tcaacagttt ttaccgaata caggttttaa cccaactgtt ggtactttcc tttttactgc
    43561 aggattgagc ttgttagttt tattggtttc taaaagggaa aatggaaaga aacgacttgt
    43621 tcattttctg ctgttgacta gcatgggagt tcaattgttg ccggccagtg cttttgggtt
    43681 gaccagccag attttatctg cctataatag tcagctttct atcggagtcg gggaacattt
    43741 accagagcct ctgaaaatcg aaggttatca atatattggt tatatcaaaa ctaagaaaca
    43801 ggataataca gagctttcaa ggacagttga tgggaaatac tctgctcaaa gagatagtca
    43861 accaaactct acaaaaacat cagatgtagt tcattcagct gatttagaat ggaaccaagg
    43921 acaggggaag gttagtttac aaggtgaagc atcaggggat gatggacttt cagaaaaatc
    43981 ttctatagca gcagacaatc tatcttctaa tgattcattc gcaagtcaag ttgagcagaa
    44041 tccggatcac aaaggagaat ctgtagttcg accaacagtg ccagaacaag gaaatcctgt
    44101 gtctgctaca acggtgcaga gtgcggaaga ggaagtattg gcgacgacaa atgatcgacc
    44161 agagtataaa cttccattgg aaaccaaagg cacgcaagaa cccggtcatg agggtgaagc
    44221 cgcagtccgt gaagacttac cagtctacac taagccacta gaaaccaaag gtacacaagg
    44281 acccggacat gaaggtgaag ctgcagttcg cgaggaagaa ccagcttaca cagaaccgtt
    44341 agcaacgaaa ggcacgcaag agccaggtca tgagggcaaa gctacagtcc gcgaagagac
    44401 tctagagtac acggaaccgg tagcgacaaa aggcacacaa gaacccgaac atgagggcga
    44461 agcggcagta gaagaagaac ttccggcttt agaggtcact acacgaaata gaacggaaat
    44521 ccagaatatt ccttatacaa cagaagaaat tcaggatcca acacttctga aaaatcgtcg
    44581 taagattgaa cgacaagggc aagcagggac acgtacaatt caatatgaag actacatcgt
    44641 aaatggtaat gtcgtagaaa ctaaagaagt gtcacgaact gaagtagctc cggtcaacga
    44701 agtcgttaaa gtaggaacac ttgtgaaagt taaacctaca gtagaaatta caaacttaac
    44761 aaaagttgag aacaaaaaat ctataactgt aagttataac ttaatagaca ctacctcagc
    44821 atatgtttct gcaaaaacgc aagttttcca tggagacaag ctagttaaag aggtggatat
    44881 agaaaatcct gccaaagagc aagtaatatc aggtttagat tactacacac cgtatacagt
    44941 taaaacacac ctaacttata atttgggtga aaataatgag gaaaatactg aaacatcaac
    45001 tcaagatttc caattagagt ataagaaaat agagattaaa gatattgatt cagtagaatt
    45061 atacggtaaa gaaaatgatc gttatcgtag atatttaagt ctaagtgaag cgccgactga
    45121 tacggctaaa tactttgtaa aagtgaaatc agatcgcttc aaagaaatgt acctacctgt
    45181 aaaatctatt acagaaaata cggatggaac gtataaagtg acggtagccg ttgatcaact
    45241 tgtcgaagaa ggtacagacg gttacaaaga tgattacaca tttactgtag ctaaatctaa
    45301 agcagagcaa ccaggagttt acacatcctt taaacagctg gtaacagcca tgcaaagcaa
    45361 tctgtctggt gtctatacat tggcttcaga tatgaccgca gatgaggtga gcttaggcga
    45421 taagcagaca agttatctca caggtgcatt tacagggagc ttgatcggtt ctgatggaac
    45481 aaaatcgtat gccatttatg atttgaaaaa accattattt gatacattaa atggtgctac
    45541 agttagagat ttggatatta aaactgtttc tgctgatagt aaagaaaatg tcgcagcgct
    45601 ggcgaaggca gcgaatagcg cgaatattaa taatgttgca gtagaaggaa aaatctcagg
    45661 tgcgaaatct gttgcgggat tagtagcgag cgcaacaaat acagtgatag aaaacagctc
    45721 gtttacaggg aaacttatcg caaatcacca ggacagtaat aaaaatgata ctggaggaat
    45781 agtaggtaat ataacaggaa atagttcgag agttaataaa gttagggtag atgccttaat
    45841 ctctactaat gcacgcaata ataaccaaac agctggaggg atagtaggta gattagaaaa
    45901 tggtgcattg atatctaatt cggttgctac tggagaaata cgaaatggtc aaggatattc
    45961 tagagtcgga ggaatagtag gatctacgtg gcaaaacggt cgagtaaata atgttgtgag
    46021 taacgtagat gttggagatg gttatgttat caccggtgat caatacgcag cagcagatgt
    46081 gaaaaatgca agtacatcag ttgataatag aaaagcagac agattcgcta caaaattatc
    46141 aaaagaccaa atagacgcga aagttgctga ttatggaatc acagtaactc ttgatgatac
    46201 tgggcaagat ttaaaacgta atctaagaga agttgattat acaagactaa ataaagcaga
    46261 agctgaaaga aaagtagctt atagcaacat agaaaaactg atgccattct acaataaaga
    46321 cctagtagtt cactatggta acaaagtagc gacaacagat aaactttaca ctacagaatt
    46381 gttagatgtt gtgccgatga aagatgatga agtagtaacg gatattaata ataagaaaaa
    46441 ttcaataaat aaagttatgt tacatttcaa agataataca gtagaatacc tagatgtaac
    46501 attcaaagaa aacttcataa acagtcaagt aatcgaatac aatgttacag gaaaagaata
    46561 tatattcaca ccagaagcat ttgtttcaga ctatacagcg ataacgaata acgtactaag
    46621 cgacttgcaa aatgtaacac ttaactcaga agctactaaa aaagtactag gagcagcgaa
    46681 tgatgcagcc ttagataacc tatacttaga tagacaattt gaagaagtta aagctaatat
    46741 agcagaacac ctaagaaaag tattagcgat ggataaatca atcaatacta caggagacgg
    46801 tgtagttgaa tacgtaagtg agaaaatcaa aaataacaaa gaagcattta tgctaggtct
    46861 tacttatatg aaccgttggt acgatattaa ttatggtaaa atgaatacaa aagatttatc
    46921 tacgtacaag tttgacttta acggaaataa tgagacttca acgttggata ctattgtcgc
    46981 attaggaaat agtggactag ataacctgag agcttcaaat actgtaggtt tatatgcgaa
    47041 taaacttgca tcggtaaaag gagaagattc agtctttgac ttcgtagaag cgtatagaaa
    47101 actgttctta ccaaacaaaa caaataacga gtggtttaaa gaaaatacaa aggcatatat
    47161 agtcgaaatg aagtctgata ttgcagaagt acgagaaaaa caagaatcac caacagccga
    47221 tagaaaatat tcattaggag tttacgatag aatatcagca ccaagttggg ggcataagag
    47281 tatgttatta ccactactaa ctttacctga agaatctgtg tatatttcat cgaatatgtc
    47341 tacacttgca ttcggttcgt atgaaagata tcgtgatagt gtggatggag ttattctttc
    47401 aggagatgct ttacgaactt atgtaagaaa tagagttgat atagcagcga aaaggcatag
    47461 agaccattat gatatttggt acaatcttct tgacagtgct tcaaaagaaa aacttttccg
    47521 ttctgtgata gtttatgatg gattcaatgt aaaagatgag acaggaagaa cttattgggc
    47581 aaggttaacg gataaaaaca tcggctctat taaagaattc ttcggacctg ttgggaaatg
    47641 gtatgagtat aatagtagtg caggagcgta tgcgaatgga agtttaacgc actttgtgtt
    47701 agatagatta ttagatgctt atggaacgtc ggtttatact catgaaatgg ttcataattc
    47761 tgattctgca atctactttg aaggaaatgg tagacgtgaa ggattgggag cggagttata
    47821 cgcacttggt ttactgcaat ctgtagatag tgtaaattct catattttag ctttaaatac
    47881 gttatataaa gcagaaaaag atgatttgaa tagattgcat acatataatc cggtggaacg
    47941 tttcgattcg gatgaggcgc ttcaaagtta tatgcatgga tcatatgatg taatgtatac
    48001 acttgatgcg atggaagcaa aagcgatatt agctcaaaat aatgatgtta agaaaaaatg
    48061 gtttagaaaa atagaaaatt attacgttcg tgatactaga cataataaag atacacatgc
    48121 aggaaataaa gtccgtccat taacagatga agaagtagct aacttaacat cgttaaactc
    48181 attaatcgac aacgacatca taaatagacg tagctatgat gataatagag aatataaacg
    48241 aaatggctac tatactataa gtatgttctc tcctgtatac gcagcgctaa gcaattcgaa
    48301 aggtgctcct ggagatatta tgtttagaaa aatagcttat gaattacttg cggaaaaagg
    48361 ttatcacaaa ggattcctac cttatgtttc taatcagtac ggagcagaag catttgccag
    48421 cggaagcaaa acattctcat catggcatgg aagagatgtt gctttagtga cagatgattt
    48481 agtatttaag aaagtattca atggtgagta ctcatcatgg gctgatttca aaaaagcaat
    48541 gtttaaacaa cgtatagata aacaagataa tctgaaacca ataacaattc aatacgaatt
    48601 aggtaatcct aatagtacaa aagaagtaac tataacaacg gctgcacaaa tgcaacaatt
    48661 aattaatgaa gcggctgcga aagatattac taatatagat cgtgcaacga gtcatacccc
    48721 agcaagttgg gtgcatttat taaaacaaaa aatctataat gcatatcttc gcactacaga
    48781 tgactttaga aattctatat ataaataaga ttgtagagtt tcattgttga gtagtgtttc
    48841 ttgtaaggat gaggagtcag atgacaaatc gactcctttt tcttatggat cgatgtagag
    48901 atttgattga atgcagattg caggaatcat cttcaactca tcaacgacca atggtgacaa
    48961 ggtggatttc aatcccacag aaaatgttga tttgagaaat aactttgcta gtctagtaaa
    49021 ataaatacaa aacaatccta gaagattttt tctgggattg ttttttgctg agtgggatgc
    49081 ttcaagttgt ctggcttgac tttcttgagg gaagttatat aatggttgta ataattaaag
    49141 ttacaataaa agaggaaata tttatgacat atgaatacca gagccacatt tatttagcag
    49201 aggcagtttt aaatgtgaag gatttggtca gtcaaacagt tttttatcag cagattattg
    49261 gtttagaaat cctatctcaa acggatacag aggtcgttct gggacttgga ggaaaagcct
    49321 tggtacactt gattcaagca caagagggtg gagaagtaag ggaacattat ggtctttacc
    49381 atctggctat tcttttgccg acacgaaagg ctttggctga tgtcttgaag cacctgacgg
    49441 atttacagat tcctcttgtt ggcggtgcag atcacggtta cagtgaggcc ctttacttag
    49501 aggacttgga gggaaatggc attgaactct atcgagataa gccagtttcc acatgggata
    49561 ttcgagaaga tggacgtatt atcggggtga ctgaagtcct tgcggctcag gatatctatg
    49621 agttggggga aagagtagag ccttttatcc tagcagaggg tacgagaatg gggcatattc
    49681 atctttctgt caaggatagt cgaaagtcca gacagtttta tcaaacggtg ttagggctcg
    49741 aggataaatt cagtgtgcct agtgctagtt ggatcgcagc tggggactac catcatcatt
    49801 tagcagtcaa cgaatgggga ggaaaaggtc tggatccgcg taaacaagtc ctaccaggtt
    49861 tagcctacta tgtcatcgaa gtcgcacata aagaagaact gttaacgatt gcccaacgag
    49921 cacaagaagt tgacgcacca atcaaatgga tgacatcgat ccaattggaa atcacagact
    49981 cagatggcat cgtgacccgt attcgtttag ctagatagat ggtatgtgat gaaggtagag
    50041 catcaattgt agaaagtgct ccttattcta gtcagatacg tggaaaattt gatatgatag
    50101 tagggaataa ggagaagaat aatatgacta gcgaatacca gaaaatgata gcaggagagt
    50161 tttaccgtcc atcagaccca gaactgagag ccttagctca ggcttctcgc caaaaacagg
    50221 ccgcctttaa caaggaagag aaccccttga agggagccga aatcatcaag acttggtttg
    50281 cctcaaccgg gaaaaatctt tacatcaaca ctcgcttgat ggtggactac ggtgtcaaca
    50341 tccatctagg ggaaaatttt tattctaatt ggaacttgac catgctggat atctgtccca
    50401 ttcgtatcgg ggacaatgct atgattggtc ctaattgtca gtttttgaca cccctccatc
    50461 cactagatcc acaggaacgc aattcaggta tcgagtacgg aaagcctatc acaatcggag
    50521 ataatttctg gactggtggt ggcgtcattg tccttcctgg agtgacactg ggaaataatg
    50581 tcgttgcagg agcaggggca gtaattacca aatcttttgg cgacaacgtt gtcctagctg
    50641 gcaatcctgc gcgcgtgatt aaggaaatac ctgttaaata gaagtaaaaa ggaacagctg
    50701 gggttgtttc ttttttgtag gtttcatcat tttttaccca gttcacattt acctactcta
    50761 tctcttagca agtctgtttc attaagcaag ttcaaagcat ctcgtaagtg ggatgttttt
    50821 ctcctcagtt catcagcttc ctccttgaca ctcggtcaga ttttgataca atagtacaaa
    50881 attagaggag gcaggctatg attcagaaac atgcgattcc tattttagag tttgatgaca
    50941 atcctcaggc ggttatcatg cccaatcacg aggggctgga cttgcagttg ccaaagaagt
    51001 gtgtttatgc atttttaggt gaggagattg accgctatgc gagggaagta ggggcgaact
    51061 gtgttggcga atttgtttct gccaccaaga cctatccagt ttatgtcgtg aactacaagg
    51121 acgaggagat ttgtctggtt caggctcctg tcggttcagc tccagcagcc cagtttatgg
    51181 attggttgat tggctatggt gtggagcaga ttatctctac tgggacctgt ggtgtcctag
    51241 ctgatataga ggaaaatgcc tttctagtcc ctgttcgcgc tctgcgagat gaaggagcca
    51301 gttaccacta tgtggcacct tctcgttata tggaaatgca gccagaggct attgctgcca
    51361 ttgagcgagt tttggatgat agagggattc cttatgaaga agtcatgacc tggtccacag
    51421 atggttttta ccgagaaacg gctgaaaagg tggcttatcg caaggaagaa gactgtgctg
    51481 ttgtggagat ggagtgttcc gctcttgcgg cagtagctca attgcgtggg gttctctggg
    51541 gtgaattgtt gttcacagca gattctctag cggacttgga ccagtacgac agtcgtgact
    51601 ggggctcgga agcttttaat aaggcgctag aactgagttt agcaagtgtt caccaccttt
    51661 agttgtactg gcaaaggatt tgttttatca taaaatgtct agctcatact tttcaaaaat
    51721 atgtttaaac gaggtcacct tcctcttgtc ctaggcatgt tgaggttggg aaaaatcttt
    51781 aaaatcagaa aaacgtatca tatcaggtga tgaaaacttt gacactatgc gttttatgtc
    51841 gataagattt agagtgagat gaaatgatac tcttcgaaaa tctcttcaaa ccaggtcagc
    51901 ttcaccttgc cgtaggtata tgttactgac ttcgtcagtt ctatttgcaa cctcaaaaca
    51961 gtgttttgag caacctgtga ctagctttct aatcgatgcc ttggttttca ttgcctataa
    52021 tcaaaaagag aaattttctc ctgaaaagca tatagagtag ctggcgttaa aagctcctgt
    52081 cttgcttttt tgacctatag tcacatctat caagtattgt tcttgcctaa gctatcaata
    52141 aaaaggtggc attttttagg cttggtgtta gtagattttg ccttatccta tctaagtcat
    52201 ttcgaacttt ttatggtaca atggaaacat gttattcaaa ttatctaagg aaaaaataga
    52261 gctaggctta tctcgtttat cgccagcccg tcgtattttt ttgagttttg ccttggtcat
    52321 tttactaggc tctcttcttt tgagcttgcc ctttgtccaa gttgaaagct cacgagcgac
    52381 ttattttgat catcttttca ctgctgtatc tgcagtctgt gtgacgggtc tctcaaccct
    52441 tccagtagct cacacctata atatatgggg tcaaataatc tgtttgctct tgattcagat
    52501 cggtggtcta gggctcatga cctttattgg ggttttctat atccagagca agcaaaagct
    52561 tagtcttcgt agccgtgcaa ctattcagga tagttttagt tatggagaaa ctcgatcttt
    52621 gagaaagttt gtctattcta tttttctcac gacctttttg gttgagagct tgggagctat
    52681 tttgcttagt tttcgcctta ttcctcaact tggctgggga cgtggtcttt ttagttccat
    52741 ttttctagcg atctcagcct tctgtaatgc cggttttgat aatttaggga gcaccagttt
    52801 atttgctttt cagaccgatt tactggtcaa tctggtgatt gcaggcttaa ttattacagg
    52861 cggccttggt tttatggtct ggtttgattt ggctggtcat gtaggaagaa agaaaaaagg
    52921 acgtctgcac tttcatacga agcttgtact attgttgact ataggtttgt tgttatttgg
    52981 aacggcaact actctctttc ttgagtggaa caatgctgga acgattggca atctccctgt
    53041 tgccgataag gttttagtta gcttttttca aacagtgacg atgcgaacag ctggcttttc
    53101 tacgatagat tatactcagg ctcatcctgt gactcttttg atttatatct tacagatgtt
    53161 tctaggtggg gcacctggag gaacagctgg gggactcaag attacgacat tttttgtcct
    53221 cttggtcttt gcacgaagtg agcttctagg cttgcctcat gccaatgttg cgagacgaac
    53281 gattgcgccg cgaacggttc aaaaatcctt tagtgtcttt attatctttt tgatgagctt
    53341 cttaatagga ttgattctgc tagggataac agccaaaggc aatcctccct ttatccacct
    53401 catatttgaa accatttcag ctcttagtac agttggtgta acggcaaatc tgactcctga
    53461 ccttgggaaa ttggctctca gtgttatcat gccacttatg tttatgggac gaattggtcc
    53521 cttgaccttg tttgttagct tggcagatta ccatccagaa aagaaagata tgattcacta
    53581 tatgaaagca gatattagta ttggttaaga aaggaaagag catgtcagat cgtacgattg
    53641 gaattttggg cttgggaatt tttgggagca gtgtcctagc tgccctagcc aagcaggata
    53701 tgaatattat cgctattgat gaccacgcag agcgcatcaa tcagtttgag ccagttttgg
    53761 cgcgtggagt gattggtgac atcacagatg aagaattatt gagatcagca gggattgata
    53821 cctgcgatac cgttgtagtc gcgacaggtg aaaatctgga gtcgagtgtg cttgcggtta
    53881 tgcactgtaa gagtttgggg gtaccgactg ttattgctaa ggtcaaaagt cagaccgcta
    53941 agaaagtgct agaaaagatt ggagctgact cggttatctc gccagagtat gaaatggggc
    54001 agtctctagc acagaccatt cttttccata atagtgttga tgtctttcag ttggataaaa
    54061 atgtgtctat cgtggagatg aaaattcctc agtcttgggc aggtcaaagt ctgagtaaat
    54121 tagacctccg tggcaaatac aatctgaata ttttgggttt ccgagagcag gaaaattccc
    54181 cattggatgt tgaatttgga ccagatgacc tcttgaaagc agatacctat attttggcag
    54241 tcatcaacaa ccagtatttg gataccctag tagcattgaa ttcgtaaaga gggatgaccc
    54301 ctcttttttg atgcccaaga tggcaaatag agacagaagc cccttgtctt ctagtaaaag
    54361 ttcttcaaag gctggacttt atggtaaaat agaaggaagt gacaagagag agtaatactc
    54421 aatgaaaatc aaagatcaaa ctaggaaact agctacgggc tgcttaaaac actgttttga
    54481 ggttgtggat agaactgacg aagtcagctc aaaacactgt tttgaggttg cagatagaac
    54541 tgacgaagtc agtaacatct atacggcaag gcgatgttga cgcggtttga agagattttc
    54601 gaagagtata agaaaaaatc agtcccctaa aggagcagat tatgaagtta ttgtctatcg
    54661 ccatttctag ctataatgca gcagcctatc ttcattactg tgtggagtcg ctagtgattg
    54721 gctgggacca ggtcggaaat ttctccaaag gccagtatat catgatgcac tcgctgattt
    54781 atcggacagc tttgttgcgt gctagccagt tctaactgcc tgaacatact ttttatgtcg
    54841 ataatctctt tgtctttacg ccccttcagc aggtcaagac catgtactat ctgcctgtcg
    54901 atttctatcg ttatttgatt gggcgtgagg accagtctgt caatgagcaa gtgatgatta
    54961 agtgcattga ccagcaactc aaggtcaatc gactcttggt agaccagctt gatttgtccc
    55021 aagtgagtca tcccaaaatg cgagaatatc tgctgaatca tattgaaatc acgacggtga
    55081 tttccagtac cctgctcaac cgatctgaaa cagcggagca tctggcaaaa aaacgccaat
    55141 tgtggaccta tattcagcag aaaaatccag aagtctttca ggctattcgt aagaccatgt
    55201 tgagccgttt gaccaaacat tctgtcttgc cagatcgcaa actgtccaat gtcgtctatc
    55261 aaatcaccaa atctgtttat ggatttaatt aatataagtg ttttataaga gggatttaag
    55321 aaaaatttta actttttctt agtccttttt aatttcagga gattatacta gagtcatcaa
    55381 ataaagaaag actctaagga gaatcctatg aaattcaatc caaatcaaag atatactcgt
    55441 tggtctattc gccgtctcag tgtcggtgtt gcctcagttg ttgtggctag tggcttcttt
    55501 gtcctagttg gtcagccaag ttctgtacgt gccgatgtgg tcaatccgac cccaggtcaa
    55561 gtcttacctg aagagacatc gggaacgaaa gagggtgact tatcagaaaa accaggagac
    55621 accgttctca ctcaagcgaa acctgagggc gttactggaa atacgaattc acttccgaca
    55681 cctacagaaa gaactgaagt gagcgaggaa acaaactctt ctagtctgga tacacttttt
    55741 gaaaaagatg aagaagctca aaaaaatcca gagctaacag atgtcttaaa agaaactgta
    55801 gatacagctg atgtggatgg gacacaagca agtctagcag aaactactcc tgaacaagta
    55861 aaaggtggag tgaaagaaaa tacaaaagac agcatcgatg ttcctgctgc ttatcttgaa
    55921 aaagctgaag ggaaaggtcc tttcactgcc ggtgtaaacc aagtaattcc ttatgaacta
    55981 ttcgctggtg atggtatgtt aactcgtcta ttactaaaag cttcggataa tgctccttgg
    56041 tctgacaatg gtactgctaa aaatcctgct ttacctcctc ttgaaggatt aacaaaaggg
    56101 aaatacttct atgaagtaga cttaaatggc aatactgttg gtaaacaagg tcaagcttta
    56161 attgatcaac ttcgcgc
//
LOCUS       DAVHEX010000007        44410 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVHEX010000007 DAVHEX010000000
VERSION     DAVHEX010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 44410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44410
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.007"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..649
                     /locus_tag="VVL45_000415"
     CDS             <1..649
                     /locus_tag="VVL45_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4186648.1"
                     /translation="GINVTIKKGETVAIVGESGSGKSTLAKILLGLLEPNIGSIEVDG
                     VEKEEIGQTLYRKIFGAVLQNSTLSYGTLRENLTFGHFVSDEELMTNLNSIGLSNVVK
                     SLPLGLETIIAEEGNNFSGGQQQMILLARCLLSKPSVVVLDEATSSLDNLSQQITTSY
                     LSEIGTTKILIAHRLDTIKSADKILVMHNGEIVEIGTHRELLELGGIYKQLYSNN"
     gene            673..2412
                     /locus_tag="VVL45_000416"
     CDS             673..2412
                     /locus_tag="VVL45_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU4186649.1"
                     /translation="MKIMKKKYWTLAILFFCLFNNSVTAQEIPKNLDGNITHTQTSES
                     FSESDEKQVDYSNKNQEEVDQNKFRIQIDKTELFVTTDKHLEKNCCKLELEPQINNDI
                     VNSESNNLLGEDNLDNKIKENVSHLDNRGGNIEHDKDNLESSIVRKYEWDIDKVTGGG
                     ESYKLYSKSNSKVSIAILDSGVDLQNTGLLKNLSNHSKNYVPNKGYLGKEEGEEGIIS
                     DIQDRLGHGTAVVAQIVGDDNINGVNPHVNINVYRIFGKSSASPDWIVKAIFDAVDDG
                     NDIINLSTGQYLMIDGEYEDGTNDFETFLKYKKAIDYANQKGVIIVAALGNDSLNVSN
                     QSDLLKLISSRKKVRKPGLVVDVPSYFSSTISVGGIDRLGNLSDFSNKGDSDAIYAPA
                     SSTLSLSELGLNNFINAEKYKEDWIFSATLGGYTYLYGNSFAAPKVSGAIAMIIDKYK
                     LKDQPYNYMFVKKILEETLPVKNGIKVLNIPNVLRYDLNMLQLEYKNEQSWDSFIDNV
                     NLIELEERIQTTIGIKQINTHNIITIAREGYSQNYLPNTSENTYNSLQVSLVGVLLLF
                     ISMVNILWAKKSK"
     gene            2463..3230
                     /locus_tag="VVL45_000417"
     CDS             2463..3230
                     /locus_tag="VVL45_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU4186650.1"
                     /translation="MSQKMNHLDVGEFVLLLPEHLRSEEEHYKSVFEDDLTSRISSQD
                     ERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQSILFWIDAVQ
                     NYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVMLAGAVLGIAT
                     SILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLGFVASVFLQVE
                     IGVAFLVLLLFTGLSLLQLHVQMQKENKMSMLVLKGG"
     gene            3232..3864
                     /locus_tag="VVL45_000418"
     CDS             3232..3864
                     /locus_tag="VVL45_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4186651.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLVYLDLDKRIFELSGGESQRVALAKIILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDHLK"
     gene            complement(4810..5223)
                     /gene="ndk"
                     /locus_tag="VVL45_000419"
     CDS             complement(4810..5223)
                     /gene="ndk"
                     /locus_tag="VVL45_000419"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEU4186652.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEIIQNVVHGSDSEESAKREIALWF"
     gene            complement(5334..9011)
                     /gene="rpoC"
                     /locus_tag="VVL45_000420"
     CDS             complement(5334..9011)
                     /gene="rpoC"
                     /locus_tag="VVL45_000420"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEU4186653.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            complement(9044..12655)
                     /gene="rpoB"
                     /locus_tag="VVL45_000421"
     CDS             complement(9044..12655)
                     /gene="rpoB"
                     /locus_tag="VVL45_000421"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU4186654.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TSEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVIAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            13071..13141
                     /locus_tag="VVL45_000422"
     tRNA            13071..13141
                     /locus_tag="VVL45_000422"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13103..13105,aa:Cys,seq:gca)
     gene            complement(13339..14670)
                     /locus_tag="VVL45_000423"
     CDS             complement(13339..14670)
                     /locus_tag="VVL45_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEU4186655.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPIIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFDVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(14899..15723)
                     /gene="endA"
                     /locus_tag="VVL45_000424"
     CDS             complement(14899..15723)
                     /gene="endA"
                     /locus_tag="VVL45_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HEU4186656.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMPSAPNSPKTNLSQKKQ
                     ASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            complement(15762..15950)
                     /locus_tag="VVL45_000425"
     CDS             complement(15762..15950)
                     /locus_tag="VVL45_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU4186657.1"
                     /translation="MNKKSSYVVKRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            complement(15943..17217)
                     /gene="murA"
                     /locus_tag="VVL45_000426"
     CDS             complement(15943..17217)
                     /gene="murA"
                     /locus_tag="VVL45_000426"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU4186658.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDDITEEAPYKYVSKMRASIVVLGPI
                     LARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHIYMD
                     FPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETITITG
                     VEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVEVIE
                     EDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFENRFQ
                     HLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETVVGK
                     LVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            complement(17241..17471)
                     /locus_tag="VVL45_000427"
     CDS             complement(17241..17471)
                     /locus_tag="VVL45_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEU4186659.1"
                     /translation="MVQLLFTLSSHMFFIYVSFYLLKDLVRWEKVLKVTDDNTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            complement(17529..18566)
                     /locus_tag="VVL45_000428"
     CDS             complement(17529..18566)
                     /locus_tag="VVL45_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU4186660.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            complement(18550..19038)
                     /gene="coaD"
                     /locus_tag="VVL45_000429"
     CDS             complement(18550..19038)
                     /gene="coaD"
                     /locus_tag="VVL45_000429"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEU4186661.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLYVGIFFNPHKQ
                     GFLPLENRKRGLEKAVKHLGNVKVVSSHDKLVVDVAKRLGATCLVRGLRNASDLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACYVPESILEEIRNEK
                     KD"
     gene            complement(19028..19567)
                     /gene="rsmD"
                     /locus_tag="VVL45_000430"
     CDS             complement(19028..19567)
                     /gene="rsmD"
                     /locus_tag="VVL45_000430"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEU4186662.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEVGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAERELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            complement(19632..20624)
                     /locus_tag="VVL45_000431"
     CDS             complement(19632..20624)
                     /locus_tag="VVL45_000431"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEU4186663.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            complement(20736..21419)
                     /locus_tag="VVL45_000432"
     CDS             complement(20736..21419)
                     /locus_tag="VVL45_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEU4186664.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYLLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            complement(21475..22215)
                     /locus_tag="VVL45_000433"
     CDS             complement(21475..22215)
                     /locus_tag="VVL45_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEU4186665.1"
                     /translation="MTIITSKANSVVKNTKKLHQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFTLENYRDQLATFSQTVWVSEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            22249..22527
                     /locus_tag="VVL45_000434"
     CDS             22249..22527
                     /locus_tag="VVL45_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEU4186666.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYPDFKIAN"
     gene            22605..23531
                     /gene="yidC"
                     /locus_tag="VVL45_000435"
     CDS             22605..23531
                     /gene="yidC"
                     /locus_tag="VVL45_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU4186667.1"
                     /translation="MKSIKRFALSAMGVAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(23606..24400)
                     /gene="pflA"
                     /locus_tag="VVL45_000436"
     CDS             complement(23606..24400)
                     /gene="pflA"
                     /locus_tag="VVL45_000436"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEU4186668.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKQLMDTESYQDYMKRVHG"
     gene            complement(24526..25776)
                     /locus_tag="VVL45_000437"
     CDS             complement(24526..25776)
                     /locus_tag="VVL45_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEU4186669.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGIHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     ISLDFINLSGGIGVNYHPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEVVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            complement(25843..26670)
                     /gene="purR"
                     /locus_tag="VVL45_000438"
     CDS             complement(25843..26670)
                     /gene="purR"
                     /locus_tag="VVL45_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEU4186670.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            complement(26937..27878)
                     /locus_tag="VVL45_000439"
     CDS             complement(26937..27878)
                     /locus_tag="VVL45_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEU4186671.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMTNKIFAMDNRSFYKPDLD"
     gene            complement(27868..29124)
                     /locus_tag="VVL45_000440"
     CDS             complement(27868..29124)
                     /locus_tag="VVL45_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEU4186672.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVVVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFARDIRNKYI
                     APPRTTNFGVLFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            complement(29126..29788)
                     /locus_tag="VVL45_000441"
     CDS             complement(29126..29788)
                     /locus_tag="VVL45_000441"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEU4186673.1"
                     /translation="MSEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLE
                     EDLPLALAVGDFDSVTEEERQVIQKGAQYFVQARPEKDDTDLELALLTIFEQNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMHQIEIEDGQNLITYCPEGISQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            complement(29751..30407)
                     /locus_tag="VVL45_000442"
     CDS             complement(29751..30407)
                     /locus_tag="VVL45_000442"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEU4186674.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRPHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTSVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGEVNERVQTLRKQLD
                     "
     gene            complement(30418..31296)
                     /gene="rsgA"
                     /locus_tag="VVL45_000443"
     CDS             complement(30418..31296)
                     /gene="rsgA"
                     /locus_tag="VVL45_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEU4186675.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            complement(31298..32170)
                     /gene="rsmA"
                     /locus_tag="VVL45_000444"
     CDS             complement(31298..32170)
                     /gene="rsmA"
                     /locus_tag="VVL45_000444"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEU4186676.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLVPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPDLPIKVVANLPYYITTPILMHLIESGIPFCEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            complement(32301..32609)
                     /locus_tag="VVL45_000445"
     CDS             complement(32301..32609)
                     /locus_tag="VVL45_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4186677.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYL
                     LDFASIEGQRQKHYRLKNLPQTVELTVDDVEEDVDLTLPENRSYQEADFFERMFRENC
                     "
     gene            complement(32602..33243)
                     /locus_tag="VVL45_000446"
     CDS             complement(32602..33243)
                     /locus_tag="VVL45_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4186678.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            complement(33245..35275)
                     /locus_tag="VVL45_000447"
     CDS             complement(33245..35275)
                     /locus_tag="VVL45_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU4186679.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETRFTYATYGEGKLPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLTATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSVYYQGLFTDYL
                     QNFSSESVEVTSQKHYLPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEKGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            complement(35544..36443)
                     /locus_tag="VVL45_000448"
     CDS             complement(35544..36443)
                     /locus_tag="VVL45_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU4186680.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLDYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMPQIIEKEAYSINDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTQIENYFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALREERDFSLAESFYQSSKTFAQ
                     LIGDEFLVKKLTEEWQEDVKKYL"
     gene            complement(36515..37075)
                     /gene="rnmV"
                     /locus_tag="VVL45_000449"
     CDS             complement(36515..37075)
                     /gene="rnmV"
                     /locus_tag="VVL45_000449"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEU4186681.1"
                     /translation="MKEKISQVIVVEGRDDTVNLKRYFDVETYETRGSAINAQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMMVIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(37075..37848)
                     /locus_tag="VVL45_000450"
     CDS             complement(37075..37848)
                     /locus_tag="VVL45_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEU4186682.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVTQMNIVGFDKPTIE
                     HALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHSKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            complement(38153..39046)
                     /locus_tag="VVL45_000451"
     CDS             complement(38153..39046)
                     /locus_tag="VVL45_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEU4186683.1"
                     /translation="MVAQDPKAQDSTKLTAEKSTVKAPAQRVDVKDITHLTDEEKVKV
                     AILQANGSALDGATINVAGDGTATITFPDGSVVTILGKDTVQQSAKGESVTQEATPEY
                     KLENTPGGDKGGNTGSSDANANEGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKN
                     AIEKAAKDKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGV
                     QAIAMVTVPKRPVAPNAAPKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALAS
                     LGLVVATSGFALLGRKTRRRK"
     gene            complement(39330..39464)
                     /gene="rpmH"
                     /locus_tag="VVL45_000452"
     CDS             complement(39330..39464)
                     /gene="rpmH"
                     /locus_tag="VVL45_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEU4186684.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            complement(39609..40823)
                     /locus_tag="VVL45_000453"
     CDS             complement(39609..40823)
                     /locus_tag="VVL45_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU4186685.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(40979..41273)
                     /locus_tag="VVL45_000454"
                     /pseudo
     CDS             complement(40979..41273)
                     /locus_tag="VVL45_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            41435..41887
                     /locus_tag="VVL45_000455"
     CDS             41435..41887
                     /locus_tag="VVL45_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU4186686.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(41922..43310)
                     /locus_tag="VVL45_000456"
     CDS             complement(41922..43310)
                     /locus_tag="VVL45_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU4186687.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     AASPSEEDFGQAVSQLHQALDKAADKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            43376..44338
                     /locus_tag="VVL45_000457"
     CDS             43376..44338
                     /locus_tag="VVL45_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEU4186688.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
BASE COUNT        12824 a         9970 c         8566 g        13050 t
ORIGIN      
        1 aggaataaat gttactatta aaaaaggaga aactgttgct attgttggag aatcaggttc
       61 aggtaagagt acattagcta aaattttatt aggtttatta gaacctaata ttggttcaat
      121 agaagttgat ggagtagaaa aagaagaaat tggtcaaaca ttgtatagaa agatttttgg
      181 agcagtgtta caaaattcaa ccctaagtta tggtacctta agagagaatt tgacatttgg
      241 acactttgtt tcagatgaag aattaatgac aaatctaaat tcaattggtc ttagcaatgt
      301 agttaaatct ttacctcttg gattagagac aatcatcgct gaagaaggta ataacttttc
      361 tggagggcag cagcaaatga tacttttagc tcgttgtctt ttgtcgaaac cttcggtagt
      421 tgttttggac gaagcaacaa gtagtttaga taatttatct caacaaatta caacttctta
      481 cttaagtgaa atcggtacca ctaagatttt aattgcccat cgactagata ctatcaagtc
      541 tgcagataag atcttagtaa tgcataatgg tgaaattgta gagattggga cccatagaga
      601 acttcttgaa ctaggaggca tttataagca attgtattca aataattagt ttttgattaa
      661 aagggtaaat ttatgaagat tatgaaaaaa aaatattgga ctttagcgat attattcttt
      721 tgtttgttca ataattctgt tactgctcaa gaaataccta aaaatcttga tggcaatata
      781 actcacactc agactagcga aagtttttct gaatctgatg aaaaacaggt tgactattct
      841 aataaaaatc aagaagaagt agaccaaaat aaatttcgta ttcaaatcga taagacagaa
      901 ttatttgtaa caacagataa acatttagaa aaaaactgtt gtaaattgga acttgaacca
      961 caaataaata acgatattgt taactctgaa agtaataatt tactaggcga agataattta
     1021 gataataaaa ttaaggaaaa tgtttctcat ctagataata gaggaggaaa tatagagcat
     1081 gacaaagata acttagaatc gtcgattgta agaaaatatg aatgggatat agataaagtt
     1141 actggtggag gcgaaagtta taaattatat tctaaaagta attctaaagt ttcaattgct
     1201 attttagatt caggagtcga tttacaaaat actggattac tgaaaaatct ttcaaatcac
     1261 tcaaaaaact atgtccccaa taaaggatat ttaggaaaag aggagggaga ggaaggaata
     1321 atatcagata ttcaagatag attaggtcat ggtacggctg ttgtagctca aattgtaggg
     1381 gatgacaata ttaatggagt aaatcctcac gttaatatta acgtctatag aatatttggt
     1441 aagtcgtcag ctagtccaga ttggattgta aaagcaattt ttgatgctgt agatgatggc
     1501 aatgatatta tcaatcttag tactggacaa tatttaatga ttgatggaga atatgaggac
     1561 ggaacaaatg attttgaaac atttttgaag tataaaaagg ctattgatta cgcgaatcaa
     1621 aaaggagtaa ttatagtagc tgcattaggg aatgactccc taaatgtatc aaatcagtca
     1681 gatttattga aacttattag ttcacgcaaa aaagtaagaa aaccaggatt agtagttgat
     1741 gttccaagtt atttctcatc tacaatttcg gtcggaggca tagatcgctt aggtaattta
     1801 tcagatttta gcaataaagg ggattctgat gcaatatatg cgcctgcaag ctcaacatta
     1861 tctctttcag aattaggact taataacttt attaatgcag aaaaatataa agaagattgg
     1921 attttttcgg caacactagg aggatatacg tatctttatg gaaactcatt tgctgctcct
     1981 aaagtttctg gtgcgattgc aatgattatt gataaataca aattaaaaga tcagccctat
     2041 aattatatgt ttgtaaaaaa aattctggaa gaaacattac cagtaaaaaa tggtataaaa
     2101 gtgttaaata taccaaacgt attgagatat gatttgaata tgttacaatt agaatataaa
     2161 aatgaacaaa gttgggatag tttcatagat aatgttaatt taattgagtt ggaagagaga
     2221 attcaaacta ctattggaat taaacaaata aacacacaca atattattac tattgcccga
     2281 gaagggtact ctcaaaatta tttacctaac acttcagaaa atacatataa ttcattacaa
     2341 gtcagtttag ttggagtatt actacttttt ataagtatgg taaatatttt atgggctaaa
     2401 aaaagtaaat gaaaataaaa tttggagccc tctgaaaaag taagtcctac agttcaacta
     2461 aaatgagtca aaagatgaat caccttgatg taggggagtt tgtcttattg ctgcctgaac
     2521 acctccgttc agaggaagaa cattataaat ctgtttttga agacgactta accagtcgca
     2581 tatctagtca agatgaacga cagcaaatga ctgctacggt aggttattta gaatcaggtc
     2641 aggatcgttt tgtgtataat acgaccccta tttcttacca gcagtttttg aaagatccaa
     2701 tcatcattgt tataacaccc caatcaactg gtccacagtc cattttgttt tggatagacg
     2761 cagtacagaa ctacgttctc tttaatcaat tgtctgatgc ccaggagctt atccagagac
     2821 aaggcattga aaattgggtc tcagaaatgc aaacaggtta ccacaactac atcacattat
     2881 tggataatat ccagagggaa cgttgggtaa tgctagcagg agctgtgctt gggattgcaa
     2941 cttcaatctt gttgtttaac actatgaata ggctctactt tgaagaattt agacgtgcca
     3001 tttttatcaa acgcattgca ggtctcaggt tcttagaaat ccatcgcact tatctctttg
     3061 ctcaactggg tgtgttttta ctgggatttg ttgcgagtgt atttcttcag gtagagatag
     3121 gagttgcttt cttagtcttg ttactcttta ctggtctatc tcttttacag ttacatgtcc
     3181 aaatgcagaa agaaaacaag atgtccatgc ttgttttgaa gggaggttaa tatgattgaa
     3241 cttaaacagg tgagtaaatc ttttggagaa cgagagttat tttcgaatct ttcaatgaca
     3301 tttgaggctg gaaaagtcta tgccttaatt ggttcaagtg gtagcggaaa aacaaccttg
     3361 atgaacatga ttgggaaatt agaaccttat gatgggacga ttttttaccg aggtaaagac
     3421 ttggccaatt ataaatcaag tgattttttc cgtcacgaat tgggctacct cttccagaac
     3481 tttggcttaa ttgaaaacca aagtattgaa gaaaacctta agctaggtct cattggtcaa
     3541 aagttgagtc ggtcggaaca gcggttgagg cagaagcagg ctttagaaca ggtcggcctg
     3601 gtttatcttg acctagataa gcgcatcttt gagttatcgg gcggagaatc gcaacgggtt
     3661 gccttggcaa aaattatctt aaagaatcca ccctttattc tggcagatga gccaacagct
     3721 tcaatagacc cagcaacctc tcagttgatt atggagattt tgctatctct tcgagatgat
     3781 aataggctaa tcattatcgc aacacataat ccggcaattt gggagatggc tgatgaagtg
     3841 ttcacgatgg atcatctgaa ataaaaatcc ttgtttttaa ttgcacgatg agttactgaa
     3901 atattatcat gaatcaagaa ttggagttaa tttagaattg tacttaattt agaattgtac
     3961 tttattaata ttgaggtaac tttttcttga taaaggaaga aataatggag aggaagttag
     4021 aatgaaaaaa ttcgacaatt atattattga gaagccttgc gattctaatt cagataaact
     4081 gcaaaaaatc ttaataattg aaagtttggt agatgatatt ttgcaatttt ctctcagaat
     4141 caataatagt gtaggagaga ttttcctcct acaaccgttt taaaagaaaa ctatctttat
     4201 tccatgttat tttgaggaag atattgtgaa agtcaaagat gatgataaag ttgagtggaa
     4261 tttgttagaa tttcaaaaat ttagagcatt tttggcttag taatctgtgt tgaaggctca
     4321 aaacctatgg taaaaaagta gctttgaaaa cgtattgcct ccaaagattt agttaaataa
     4381 tgatttaaca caaaaagaaa ttattgaagt tctggaaaga tgttgtttca gtattgagaa
     4441 aaggtgggaa aaacttgcga ttttcacaga gaaaggaaga aaaagtatag aaatatagtc
     4501 aattgaaaca agaacaggat aaaagaacct tttgtgccat atttttctcc tttcgcttta
     4561 caattggatt gaacaccttt attgtatcgc gtttggagtt tttttggtat aaccttcgac
     4621 gcacacccgc atagcgggtg ttttttttgt ctcgcaccta acggagcgag acaaactaat
     4681 agtcacttaa tcaaaaaacg caccatatca aaaactaaaa agtttgatat catgcgtcat
     4741 gtcttaaact aattgactat actttctatt caaatgagct tttaaccaat tgattgagcc
     4801 aatccactct taaaaccaaa gagcaatttc tcgcttagct gactcttctg aatctgaacc
     4861 atgtacaaca ttttggataa tctcattttc tccagcagct tttgcaaaat cacctcgaat
     4921 agtgcctggt aaagcttctt ctggacgagt tgcacccatc atggtccgcc aagtttcgat
     4981 tactttggga ccagaaatga cacccacaag aactggacct gaagtcatga attcacgaat
     5041 cggtgggtaa aaactctgac caaccaagtc ctgatagtgc tggtcaatca actcttctga
     5101 aacctgtgaa cgaaactcca atttttcgat tgtaaatcca cgttgttcga tgcgctttaa
     5161 cacttcaccc actagccctc tttttacacc atctggtttg atgataaaga atgtttgttc
     5221 catacccgtc tcctttgtca gcttctttct tttattttac cacatttcgt ggaaaaatgg
     5281 agaaagtttt cagaagagag aatgagagaa ccctcgggtt ctctcattct ctcttattct
     5341 actgtttctt ccacagtttc aacggcagta tccacaacta cttctgttgt ttcttcattt
     5401 ccttcttcct ctactggagg attaaggtat tcttcttcgt tgacagcatg tggttcaagg
     5461 ttacggtaac gggccatacc agtaccagct gggatgatct taccgatgat aacattttct
     5521 ttaagtccaa ggagatggtc tttcttacca cggatagctg cgtcagtaag gacacgagtt
     5581 gtttcctgga aggaagccgc tgacaagaaa ctgtttgttt caagtgaggc tttggtaatt
     5641 cccataagga ctgggcgacc tgtcgctgga actccacctg cgataaggac atctttgttg
     5701 gcatctgtaa agtcattgat atccatgagg gtacccatga gaagatctgt atcacctgga
     5761 tccatgacac ggactttacg gatcatttga cgaaccatta cctcgatgtg tttgtcaccg
     5821 atttctaccc cttggctacg gtaaactttt tgtacttcac cgagaaggta cgtttcaact
     5881 gacaagacat cacgaactgc aaggagacgt tttggttgga tagaaccttc tgtcagagca
     5941 gcaccacgcg ctacttggcc cccaacttcg acacgcatac gagctgtaaa tggaacgaca
     6001 tattcacctt cgccagtttc acccttaaca aagactttct tggtacgagt tgatgcatct
     6061 tcttcgatag cagtaacttg tcctttaacc tctgtaataa ccgcttcccc tttaggattg
     6121 cgggcttcaa agatttcttg gacacgagga agaccctgag tgatatcggt atttgaggca
     6181 accccacctg tgtggaaggt acgcattgta agctgtgtac caggttcccc gatagattgg
     6241 gcagcgattg taccaactgc ttcaccaact tcaaccgcat caccagtcgc caagttgata
     6301 ccgtaacagt gacggcagac accgtgacga gtgttacatg taaatacaga acggatagtc
     6361 acttcttcca caccagcatt gacaatttca cgcgccttgt cttctgtaat caattcattt
     6421 ggaccaataa tcactgcacc agtttctgga tgtttaacag ttttcttagt gtaacgaccg
     6481 ttgagacgct cttcgagaga ctcgatcatc tcttttcctt ctgcgataga acggatcaag
     6541 agaccacggt cagttccaca gtcgtcctca cggatgataa cgtcttgggc aacgtcgacc
     6601 aaacgacgag tcaagtaacc tgagtcggct gtcttaaggg ccgtatcggt cataccttta
     6661 cgagcaccgt gagttgagaa gaacatttcc aataccgaca aaccttcgcg gaagtttgaa
     6721 aggattggca attccatgat acgtccattc ggagcagcca tcagaccacg cataccggca
     6781 agctgtgaga agtttgagat gttaccacgg gctccagagt ccatcatcat aacgattggg
     6841 ttcttaggat cttggttagc aatcaagcgt ttctcaagtt tttcacgggc agcacgccat
     6901 tcagctgtaa cagcattgta acgctcgtcg tctgtgatca taccacgacg gaattgtttg
     6961 gtgatttgtt cgacacgttt gtgtgattct tcaatgattt cagccttgtc atcaacgact
     7021 gggatatcgg caatacccac tgtcaatcct gcaagagttg agtggtggta accgaggttc
     7081 ttcatgcggt caagtagggc agaagtttct gtcgtacgga aacgtttgaa gatttcagcg
     7141 atgatatttc caaggttttt cttcttgaat ggagggttga gctcaagatt gctgatagct
     7201 tccttgatat ctccaccaag tggcaagaag tatttagctg gaacaccttc tgtcaagttg
     7261 gcattgtttg gttcttgcaa gtatggtagc ccctctggca tgatatcgtt gaagagaatt
     7321 ttaccaactg ttgtaagcaa gaccttatgt ctttgctctt ctgtccaagg cttgttgagg
     7381 ctgtctgttg cgataccaac acgtgagtgg aggtgaacat aaccattgcg gtaagccata
     7441 accgcttcgt cacggtcttt gaagaccatt ccttcacctt cgcgaccagc ttcttccatg
     7501 gtcaagtagt agttacccaa aaccatgtcc tgagatggag taactaccgg tttcccatct
     7561 ttcgggttca agatgtgctc agcagctagc atgaggatac gagcttctgc ttgtgcttct
     7621 tctgaaagtg gtacgtggat ggccatttgg tccccgtcaa agtcagcatt gtaggcttca
     7681 cagacaagtg ggtgcaagcg aagagcctta ccatcaatca agactggctc gaaggcttgg
     7741 atacccaaac ggtgaagggt cggtgcgcgg ttcaaaagca ctgggtgttc tttaatcact
     7801 tcttcaagga tatcccagat acgctcatct ccgcgttcca ccaagcgttt agctgctttg
     7861 acgttttgca cgatatcacg ggcaacgatt tcacgcatga caaatggttt aaagagttca
     7921 atcgccattt cacgcggcac accacattgg tacatcttaa gagttggacc aacggcgata
     7981 actgaacgtc ctgagaagtc aacacgttta ccgagcaagt tttgacggaa gcgtccttgt
     8041 ttacctttaa gcatgtggct caatgatttc aatggacggc tacctggtcc tgtgattgga
     8101 cgaccacgac gaccattgtc aatcaaagcg tcaactgctt cttgaagcat acgcttctca
     8161 ttttgaacga tgatacctgg tgcatttaac tcaagcaaac gagccaaacg gttgttacgg
     8221 ttgataacac ggcggtaaag gtcattcaag tcagatgagg caaaacggcc accatccaac
     8281 tgcaacattg gacgaagatc tggtgggata accggaagga tgttaagaat catccattca
     8341 ggtttgtttc cagacttgta aaaggcatcc aaaacatcca aacgacggat ggctttgaca
     8401 cgcttttgtc cagtagctgt tttcaattct tctttgagtt cagcaatttc tttttcaaga
     8461 tctacttgct tcaaaaggtc ttggatggct tccgcaccca tcttggcaac aaatgaacca
     8521 taaccatatt cacgcaagcg ctctcggtat tcgcgctctg tcatgataga cttgtgctca
     8581 agtggtgtat ccttaggatc aatcaccaca taagccgcaa agtagataac ttcctcgagg
     8641 gcacgagggc tcatatcaag ggtcaagccc atacggcttg gaatcccctt gaagtaccag
     8701 atgtgagata caggagcttt caattcgata tgtcccatac gctcacgacg aactttcgta
     8761 cgcgttactt caaccccaca gcggtcacaa acaattcctc tgtaacgaat gcgtttgtac
     8821 ttaccacaag cacattccca gtcttttgta ggaccaaaga tcacttcatc aaagagtcct
     8881 tcacgttctg gtttcaaggt acgataattg attgtttcag gttttttgac ttctccataa
     8941 gaccatgaac ggactttact tggagaagct agggtgattt gcatactttt aaaacgattt
     9001 acatcaacca ctatttcttc cctttctatt ctaagtgaac tgcttattct tgttcagcag
     9061 cttcttctgt tgcttccgct tttgttgctt tctcagcttc ttcagcttca aaggctgctt
     9121 tagcctcttg ggctgctttt tcgcgggctt tttcaaggtc atctacgtgg atgacatctt
     9181 cgtccattcc ttcatccaag tcgcgaagtt ctacttcttg gtcatcttcg tctaggacac
     9241 gcatgtcaag accaagagat tgcaattctt tgacaagaac tcggaaggat tctggaacac
     9301 ctggttttgg aattggtttg ccttttgtaa tagcttcata ggctttcaaa cgtccgttga
     9361 tatcgtccga cttgtaagtc aagatttctt gaaggacatt tgacgcaccg taggcttcaa
     9421 gagcccaaac ctccatctca ccgaaacgtt gtccaccaaa ctgagcctta cctccgagtg
     9481 gttgttgggt aacagttgag tatggtccga ctgaacgcgc gtgcaattta tcatcaacca
     9541 tgtggtggag tttgatcatg tacatgactc cgacagaaac acggttatca aacggttcac
     9601 cagtacgtcc atcgtaaagg atcgttttgg catcgctatc catacctgct tctttaacag
     9661 ttgaccaaag atcttcagaa cttgctccat caaagactgg tgtagcgatg tgaataccaa
     9721 gagtacgagc tgccatacca aggtgaagct ccataacctg accgatattc atacgtgatg
     9781 gtaccccaag tgggttcaac atgatgtcga ctggagttcc gtctggaagg taaggcatgt
     9841 cttctacagg aacgatacga gagacaaccc ctttgtttcc gtgacgtccg gccattttat
     9901 ctccgacctt aatcttacgt ttttgagcga tgtaaacacg aaccaacatg ttaacacctg
     9961 attgcaactc atctccattt acacgtgtaa agatcttaac atcacgaacg acaccatcgg
    10021 caccgtgtgg tacacgaaga gaagtatcac gcacttcacg agacttgtct ccaaagatag
    10081 cgtgcaagag acgttcttca gctgaaagat ctttctcacc cttaggtgtt actttaccta
    10141 caagaatatc accttcttta acctcagcac caatacggat aatccccatt tcgtcaaggt
    10201 ctttgagggc atcttcacca acgtttggaa tttcgcgagt gatttcttca ggcccaagct
    10261 ttgtatcgcg cgtttctgat tcgtattctt caaggtgaac agatgtgtag acatcgtcct
    10321 tcaccaagcg ttcgctcatg ataacggcat cctcgaagtt gtaaccttcc caagtcatgt
    10381 aggcaacgat tgggttttgt ccaagcgcca tttctccatt ttccatagaa ggtccgtcag
    10441 cgatgaaatc gcctttttca acgacatcac caacttttac gagagtgcgt tggttgtaag
    10501 cagtacctga gtttgaacga cggaattttt ggatgtggta aacatccaat gaaccatctt
    10561 cacgacgaac ttctaccttg tcagcatctg cgtaagtaac tttaccatca tactgagcaa
    10621 tcacagccgc accagaatcg tgggctgctt ggtattccat accagtacca acgtaaggtg
    10681 cctgaggatt aatcaatggc acagcctgac gttgcatatt ggctcccatg agggcacggt
    10741 tggagtcatc gttttccaag aaaggaatac atgctgtcgc aacggcaact acctgttttg
    10801 gtgaaacgtc catgtagtca acaatattag ctggatactc ttggttgacc ccttggtgac
    10861 gtcccatgac aatcttctca gcaaaggttc catcttcatt cagacgagag ttagcctgag
    10921 ctacagtata ttcatcttct tcatcagctg tcaaccaaac aatttcgttc gtgacaacac
    10981 ctgtttcacg gtcaacctta cggtatggtg tttgaacaaa accatatttg ttcaagtgtc
    11041 cataagatga caagttattg atcaaaccga tgttaggtcc ttcaggtgtc tcgattggac
    11101 acatacgacc atagtgagtg tagtgcacgt cacgtacttc atatccagca cggtcacgag
    11161 tcaaaccacc aggtcctaag gctgacaaac ggcgtttgtg agacaactca gaaagcgggt
    11221 tgtgttggtc catgaactgt gacaactgtg atgaaccaaa gaattcttta actgcagctg
    11281 ttacaggacg gatattgata atttgttgtg gtgtcaagac ttcattgtcc tgaacagaca
    11341 tacgttcacg gacattacgt tccatacgag aaagtcccaa acgtacttgg ttggcaagca
    11401 attcaccaac cgcacggata cgacgatttc caaggtggtc gatatcatct acacggccaa
    11461 gtccttcagc caagttgagg aagtagctca tctcagcaag gatatctgca ggagtcaccg
    11521 tacgaacctt gtcatctggg ttagcattac caatgatcgt tacgacgcga tctggatcag
    11581 ttggagcaat aaccttgaat ttttgaagaa caacaggctc agtcacaacg gctgcatcgt
    11641 ttgggatgta gacaatcttg ttcaagtcgc catccaaatg gctttcaatg ctttcaatca
    11701 cgctacgagt cataatcgta ccagcttcta ccaagatttc tccagtttca gggtctacca
    11761 atggctctgc aatggtttgg ttgagcaaac gtgttttaac attgagtttt ttattgattt
    11821 tgtaacgacc aactgctgcc aagtcataac gacgtgggtc aaagaagcga gctacaagca
    11881 agctacgtga gctttcagcc gtcttaggct cacctggacg aaggcgttcg taaatttctt
    11941 tcaaggcttc gtctgtacga gagtccattg gattcttgtg gatatctttt tcaacagtgt
    12001 tgcgaaccaa ttcgctgtca ccaaagatat caaagatttc atcatcacct gagaaaccaa
    12061 gagcacgaac caaggttgta aatggaatct tacgagtacg gtcgatacga gtgtaggtga
    12121 tatcttttga gtcgctttca agttccaacc aagctccacg gttagggata acagttgaac
    12181 catagcccac cttaccattt ttgtctactt tgtcgttaaa gtaaacacct ggtgagcgga
    12241 ccaactgaga aacgataata cgttcaccac cattgatgat gaaagtaccc atttctgtca
    12301 tgattgggaa atcaccaaag aaaacttctt gggtcttgat ttcgcttgtt tctttattga
    12361 tcaaacggaa ggttacaaaa attggtgctg agtagctagc atcgtggata cgagcttctt
    12421 ctagcgtata ttttggttcc ttgatttcat atccaacaaa ttccaactcc attgtgtctg
    12481 tgaagtttga aattggcaat acatcttcaa acacttcctt aagaccgtgg tctaggaaag
    12541 ctttgaatga gtcagtttga atttcaatca aatttggtaa gtcaagaact tctttgattc
    12601 ttgaaaaact acgacgggta cgatgtttcc cgtattgaac gtcatgtcct gccaagatga
    12661 ttctcctttg taaataagtt ccaagccttg tcaatcaggc ttttctaatc gtcatatggt
    12721 tgtaaacccc ttatcaccgt gtcctcttga cgaattttca gaatctttaa gcctctgtta
    12781 caaatgctca aaatcttgaa aaaaagcaca aaaagagcag ctaaatctga ctttttcaga
    12841 agatttaact gctgtgagcc ttgtctggac aatatttcag acaaaaccta cgacaaatga
    12901 ttacccatat tataccctat ttagctagat ttttcaaggg gtttcagtag gtttttggta
    12961 aattttttcc catagaaaac ttggcatcac attcgaatca cgctatggta caaaaaactg
    13021 aaaaaactat tgactgaaaa tcattttcaa ggtataataa taaacgttaa ggcggtatag
    13081 ccaagtggta aggcacggct ctgcaaaagc ttgatcgtcg gttcaaatcc gtctaccgcc
    13141 ttctataact tgatttatca ggtttcaaat gaacagaaag cccaatttga agggcttttt
    13201 ttattttccc tcgaataaat acgtataact ttaaaaactt ttggagcgag tttgtggcag
    13261 agttctttcc atggcataat tcccttttga aatcaattga aaaaagcttc gtcacgcctg
    13321 gtgacaaaag cctctagttt attctgtttc ttcttctatt tcgacttctg tgatttggac
    13381 ttttaccttg gtaacacgtc cattcttcac tttatcattg gttaagataa actgtttgtt
    13441 ttggctgacc aattcatagc tgagtttctc agtcgttgga atagttccca ctcctgtcaa
    13501 ataataacca gcgatagtat caacgtcatc actttccagt tcaacatcaa agtaattatt
    13561 gaaatcattg agggtcatag ttccttgaac aatgtaggta tcctcgccaa tctgatgaac
    13621 atctattgct gctttgtccg tttcatcatc aatttcacca acaatttcct ctaagaggtc
    13681 ttccaaagtc accaaaccag ccataccacc gtattcatcc agcaagattg ccatttgtct
    13741 ttgggtattt cgcagttctt ttagcaagtc atccacaaaa atagtttcag gtacaaaaag
    13801 tggatcttgt aaaattctct tccaaacaat attgtcaaaa ccgtccacaa agcctgcctt
    13861 aaggagactc ttggtgtgaa tgattccaat tacattgtcc ttatccccat cataaaccgg
    13921 gatacgagaa taattttgtt ttaaaatact ttggataatg gcttgactat catcctgaat
    13981 atccaccata aaggcatccg ttcgaggaac cataacctct cgtgccatca gttcatcgag
    14041 cgaaaagaca ccttgtagca tctcaatctc atcagcatcc aatgtttctt cactatttgt
    14101 cagcatatag gcaatttcat cacgggtcat cttttcatcc gcatcatcga atgacatagg
    14161 agtcaaatgg ctcaagaaat tggtcgaagc agctaaaagc caaacaaaag gactgactag
    14221 ttttccgatc ccaatgataa tcggcgctgt acgaattgcc aaggcatcct ttagattaag
    14281 agcgattctc ttaggatata attccccaaa aacgatggaa atataggtca aaaatgccaa
    14341 ggatagaaaa gttgccacgg cttgtgctgt ttcgccattc ccaagccaag aggcaatcac
    14401 acgtcctaga gtatcagtta aactcgcccc tgataagatt gtaatcaggg tgattcctac
    14461 ctggatggtt gataaaaagt ggttaggatt ttctagtacc ttcagcaggc ggatgtagcg
    14521 tctgtctcct tcttccgcct tttgttcaac tcgggcacga ttaagagaaa ccatggccat
    14581 ttctgtggct gagaaaaagg catttaatac cgtcaagata aacaataaaa caaattgtag
    14641 caacaaattc tgactacttg ggtcttccat agttcttctc ctaaaatagt atcttatcct
    14701 ttattatatc acaaaattta atccaataca tattattttt tcttaccgta acttctcaaa
    14761 gtaacttcca tctctctccc aaaactggaa gttagtttta gaagttgcta ttttttctta
    14821 gtacctgttc tagtcctgct taaactagta aaaagaactc ctacatccat aagtgacata
    14881 ggagtttagg cttatctttt actgagttac agttacttct ccagttcggt aatccagttg
    14941 aagtcccttt tgaacattgg gaactagaac attgaattcc aattctccat ccgaagactt
    15001 ggcttcaatc tgtgaagctg agggaactaa atcctcgttt gaagcgtagt aaagggttac
    15061 acggtaacgg acacgcttgt tttggtccaa ggctttacgc accttgcttt catagtagtt
    15121 ttgaccagtc gaatactcgg cttgtgcctg atttgcccag gctgtctgaa cagcaatgtt
    15181 tttaggattg cttgttgagg catcaaaacc atccaaacca ccgattaagg catagcctaa
    15241 caaatgacct ctatcgactg catgggtata agagcccttt agattcttga cctgatgcca
    15301 acctggagga gtccaagaag ttgaaccatt cccagtttct ttacgattct tgtactgacg
    15361 agtggcctta gacaagaggg cattagctac ggttggaaca gtttccttgc ccactgtctt
    15421 tgttttattg tcagcgtagg gcttacttga aaccttggca tctagatttg ttttattacc
    15481 attgacgata aaagcacctg agccattcca ctccagactc ccctttattt gactcttgac
    15541 tgcgtctgtt aagacactct ctgccaatgc ttgactagga gcttcagacg cttgtttttt
    15601 ctgactaaga ttggttttgg gactattagg tgccgacggc atctgcttga tataatagct
    15661 ccctgtagac aaaagcaata acactagcag tccgattagt gtctgtcttg tttttttgtt
    15721 catatttctc cttatcttta gaaaaaggct ggttctccag cctaatttcc tgtaaattta
    15781 tgaatcaatt cctgccattt tgctggagac aggatagccc atggatcttg acccttgccc
    15841 aagattccat aacctaccat taaaccgatt cctagagcca gagtacctaa aatcagtact
    15901 atgatgacta aaagtaaacg cttgactacg tagcttgatt tcttattcat cttcatcact
    15961 tgcctcaatc cgctgaatct tagcacctag ctgcgccaac ttctcatgga aaccgtagta
    16021 acctctatcc aagtgaacca atttaccgac cacagtttct ccctgtgcta ccaaacctgt
    16081 caaaatcaag gccgcactgg cacgaaggtc agttgaaaga acttctgctc cctgcaaagg
    16141 ctgtccacca acaatacgag ctgtatcacg gataatctca gaatgcaagc ccatgcggcg
    16201 catctcttct aggtgttgga aacgattttc gaaaactgtc tccaccatgg ttgattcgcc
    16261 ttttgcaact gtcatcaagg ctgtaaattg agcctgcata tctgttggaa atcctgggtg
    16321 gggcaaggtt ttcacatgaa cagcttttag attttctagt tgagaacgaa cacgaattcc
    16381 ttcgtcttct tcaattactt caacacccat ttcaagtaac ttggcaatca agggacggtt
    16441 gtgctcccag acagcgtctc gaatcaagac atcaccacca gtcatggcag cagctaccat
    16501 aaaggttcct gcttcgatac ggtcttggac tacattgtga gtcgtaccat gaagtttctc
    16561 aacaccagta atggttatag tctctgtacc agcacctttg accttggctc ccatttcatt
    16621 aaggagaatg gctaagtcaa caatctcagg ctcacgcgca gcattctcaa tcactgtcac
    16681 cccatcagcc agagtcgctg ccatcatcaa gttctgcgtt gcaccaacac ttggaaagtc
    16741 catatagata tgagcaccat gcaagcgttc tgccttggct tcgatgtaac cagctgtctg
    16801 actaatctta acccccatag cttccagacc tttcaaatga agatcaatag gacggctacc
    16861 aatcgtacaa ccacctggca tggatacctt ggcatgaccc acacgggcaa ggattggccc
    16921 taatacaacg atggaggcgc gcatcttgct gacatacttg taaggggctt cctcagtgat
    16981 gtcatccacc ttgacaagat gagcttcctc atcaaagtca accttggcat tcaaaccacc
    17041 aactacctga ttcataataa agacatccga caaaatcgga acattctgca agacggtctt
    17101 tccttcactt gctagaatag tcgctgccaa caagggtaag actgcatttt ttgctccctc
    17161 gatcgtcacg cttcctacca gacgattatc gccaccttga accacaattt tatccatact
    17221 cgtttccttt actcttgatt tcataataat cctctaagcg cttcttgcca caaatgatac
    17281 aggctaataa agaaagaact caggatgtag cctatgacaa tgctaaaaaa ggctactaat
    17341 aaacgaactt ttcttgtatt atcatctgtc acttttaaaa ccttctccca tctaacaaga
    17401 tcctttaaaa ggtaaaaact cacataaata aagaacatat gactacttag agtgaataat
    17461 aattgaacca tttgactatt ataccatctt ctctgtttgt gcgctagttt ggaaacttca
    17521 ctcaaaaatt agggattgtt tttcaagtaa tcaatcgcat cttgtagggt tttaacgggc
    17581 acgattttca tatccgtctt gattgtttta gccgcttcta gggctgtttg gtagttgttt
    17641 ttcgcgtccg gatgcgcctt ttgttcttct tcgctaacag ggttatcagg agcaaagaaa
    17701 atagcagcac ctgccctagc cgaagctaca accttcttat caatacctcc aatgtctccc
    17761 acattaccat cgcggtcaat ggtacctgta ccggcaacaa tacgaccatt acgaagatct
    17821 gggtgagcta tttgagtata gatagctaga ctaaacatga gaccagcact tggaccgcca
    17881 ataccagctg ttgaaaagct aattgggaca ttgctgatta cctctgtacg gtcaatcaag
    17941 ccgattccaa ttccattttt gccattttcc aaggtgatga tttttccttc tgcagacttg
    18001 gtttgcccat cctcttcata ggtgaccttg acggaatccc ctaatttttg agaactgacg
    18061 taatcaatca agtctttgga actatcaaag gtctgatcat tgactgctgt gactgtatca
    18121 gagatattga gaatcccttt aaaggttgaa ttatccgtca cattcaaaac ataaactcca
    18181 aagtacttga gttcgatatc cttaccagct gtttttagtc cttgatactt ggccatattt
    18241 tgcgatgttt gcatgtagaa ttgattgatt cgcataaatt caacatcgga agaaccacct
    18301 gtagtctcct gagcactacg aatatctgta aaaggtgtca accaagcata aatcatatga
    18361 gctaaagtgg catgttgaac accaaccgta acgaattgat aggcaccagc ttccttatct
    18421 tctgtgtcat ttactttaag gacttggcga atatcttccg aaccacctgg aacctctata
    18481 taataaggca agggcactac aaatgccaag aaagtcacaa tcaaggccgc aatgacgtat
    18541 aagggccatc taatcttttt tttcatttct tatttcctcc aaaatactct cgggaacata
    18601 gcaggcaata tcctgaccaa acttcaaaag ctctctaacg cctgatgaac tgatatagag
    18661 atgttcaggt cgactatgta aataaatagt ctctatatca gaagacagct gatgattgta
    18721 gtaatcaaaa ctggcttcat attgcaaatc cgacgcattt ctcaaacctc gcactaggca
    18781 agtagccccc agtctttttg cgacatcgac caccaattta tcatgagaag acacgacttt
    18841 aacatttccc aaatgtttca cagccttttc taacccccgt ttacgatttt caagagggag
    18901 aaatccttgt ttgtggggat taaaaaaaat acccacataa agcttatcaa aaagtctgct
    18961 cgcccgttca atgatatcca gatgcccatt tgtcatcgga tcaaatgagc ctgtgaataa
    19021 gccaatctta tctgacatag actgtcacct tactaattcc ataaatcttt tccttccaga
    19081 tacccagaca ggcaatttct tctggaagtt caacggcttt atccgtctcg cacacaacca
    19141 taacatcttc agaaaaaagc tctctctcag ccattttttc aatatctgct acgatttgtt
    19201 ccttggcata gggagggtct aagaaaacga ggtcaaattc cccagatacc tgttccaatg
    19261 ccctttctgc atccatcttg aggagttgaa attttccaac ttccttggtc atctggatat
    19321 tttcagccac gatggtctga gccttacggt ctcgctccac caaaacagca ctggacatgc
    19381 cacgcgatac tgcttcgata gataaaccac cactacctgc ataaaggtcc aagactcgtc
    19441 ccacttcaaa gtagggacca atcatgttaa aaatggctcc cctaacctta tccgaagtag
    19501 gtcttgtcgt cttgccttct agtgtcttga ggggacgtcc cccatagatt cctgatacga
    19561 ttttcatact gtttattata ccaaattata gacaaaaaga gaaagaaaac cgaaccttgc
    19621 ggttcgattc tctacaaaat attttcgtaa gtatcgcgga cttcttgagg ccaaacactt
    19681 gtttgcactt ctccgatgtg tctcttgcga agtaggaaca tggccatacg agattgtcca
    19741 attcctccac cgattgtcaa tgggaataga ccattcaaca aagacttgtg ccattccaat
    19801 tccaagcggt cttcatcacc tgtaatttcc acctgacgtc taagagtttc ttcatctaca
    19861 cgaattccca tagaagacaa ctcaaaggct ccacctaaag actcattcca gacaagaata
    19921 tcaccattta gacccttgta gccattctca gactcgcttg tccagtcatc atagtctggt
    19981 gcacgtccat cgtgcggttt accatctggc aactcgccac cgataccaat caaaaagacg
    20041 gctccaaatt ctttacaaat cgcattttca cgttctttcg gtgtcaagtc tgggtagcgt
    20101 tctaccaatt cttctgtatg gataaaggta atttgttttg gcaagataga ctcgatgtca
    20161 tagcgggctt caacagctag ctcagtcagg cgaatagcct tataaatctt ctcaactgtt
    20221 tcttttagat aaacgatgtt acgcttacca tttgggataa ccttctccca gtcccactgg
    20281 tcaacataaa cagagtgggt tgcatccaag gaatcctcat ctggacgaag ggctttcatg
    20341 tggacaaaga gaccctctcc ttcaccaaag ccaaaacgag ccaaggtgtg gcgtttccat
    20401 ttagcaagtg agtgcaccac ttcataagta gcatcaggga tttggagaac cttgaccgat
    20461 actggatttt ccacaccaga caggttgtcc tgcattccgt caccgacctt actcaagata
    20521 ggaccttgaa cttcgacaac ttctagctta tctttcaaat actgggtaaa agtgtttttg
    20581 acaaaggaaa tttcttcttg ttgatggata aaacttttct tcatagacaa ctcctcaaaa
    20641 atttaataaa cgtattatac acctatttcc aagaaaagaa aagagaaaat ttaaaaaaaa
    20701 tcagtgtcac tcaaaacact gatttcatag accttttagt cattgcgacc aaagatacgt
    20761 agaatactta ggaaaagatt gataaaatca agatagatgc tgagagccat tgacaccacc
    20821 cagcctgtcg ctacacgacc ttgtgattgt tcataggcga ggcgaatctt ttggttatcc
    20881 caagcaatca accctgaaaa aaccaaaacc atggctacgc taatcatata atcaaagaag
    20941 ccactagcca agaaaatatt gactaccata gcaatcagta aaccaatgag agctgccatc
    21001 attgctcgac caatcccact caagtctttc ttggtaaaga taccaactgc cgccatgaca
    21061 aagaagagaa gggcgctcga tacaaaggca gataaaactg tacccggagt atagaaagcc
    21121 accacaaaac taagggtaaa gccgtttaaa acggagtaaa gtaaaaatac tggaagagca
    21181 gctggactat ttcttgaagc catgctactg gcaacgaaaa ccaaagccaa ctccgcaaaa
    21241 gtagcaatgg tcaaccagag acgcccctgc atcaacaagt aaaccaactg agactgaaag
    21301 accgtcaaca taaggcctga taccaaagca gatagtccga ttcccagacc aacaaaggca
    21361 taaaccttag cgtaaaattg attgagacct gcacggtcat gtataatagt gtgattcatc
    21421 catttctcct tttctgtgaa catctttcct tgattataac aaaaaaagtt aaaattagct
    21481 taaatggaag attaaaatac cggctgcaac cgcaacattc aaactctcgg cctgcccctt
    21541 catgctaata tggaccaact ggtctgcact ttcagccata aggggactaa ttccttgacc
    21601 ctcatttccc ataactagta caaaattttc tatagaaggc agttctctgt aatcaacaga
    21661 atctttagat agggttgttg ctaacactgg gatagctacc tttttagttt cgtcaagaag
    21721 cgcttgacta gtcatccggt aaatgggcag atggaaatga ctaccttgca tggaacgtag
    21781 ggttttgaga ctgtagatgt ctgccgactt atctgaaaca atcactcctg taaaacctgc
    21841 tgcatccgca gttcgaatga tagttcctac attaccagga tcttgcacat cttccaaaaa
    21901 caagaacttg ccctgactca aatcagcttg tcctacttct tctttttgaa ccacggccac
    21961 aattccctgt ggagtctgag aatccgccaa atctagcaaa atatcctctg acacccagac
    22021 agtttgcgaa aaagttgcta actgatctcg ataattttct agggtaaaga ttttctcaat
    22081 cgtcactcca gcttgaacag cttcttcaaa caagtgccag ccttcaatca aataggctga
    22141 cttgcggtat tttttttggt gtaatttctt ggtatttttt accacagaat tggcttttga
    22201 ggttataata gtcatagaaa tattataaca caatcaaagg ggtttggtat gcaaaaggtt
    22261 agaatgattg cccaaggtag ggtgcaggga gtcggctttc gttggggtgt ttatagcctg
    22321 gcccttgaaa tcggtggcat cacaggtcgg gtatggaata acgacgatgg cacagtggaa
    22381 atcttagccc aagcagactc atcagctatt atggcaaaat ttatccaaga aatccgaaaa
    22441 ggaccgacac ctttttcaaa agtcagttac ttagatgtca aactgagcaa ctttcctccc
    22501 taccctgact ttaaaatcgc aaattaggtc tccagaacta ttgtatattt tgtaaaaaaa
    22561 cagtagaata gaaaggtata atttttaaag aaggaataaa aacagtgaaa tctattaaac
    22621 gttttgcact ctcggctatg ggagtggcta tgttgcttgt cttgactggc tgtgtcaatg
    22681 tcgataaaac cacaggtcag ccaacaggat ttatttggaa tacgatcgga gcgcctatgg
    22741 ctgaagccat caagtacttc gctactgata aaggtctagg ctttggtgtc gctatcatta
    22801 tcgtaaccat tatcgtacgc ttgattatct taccacttgg tatctaccaa tcatggaagg
    22861 caacgcttca ctctgaaaag atgaacgccc tcaagcacgt ccttgagcca caccaaacgc
    22921 gtctcaaaga agcgactact caagaagaaa aactcgaagc ccaacaagct ctctttgctg
    22981 ctcaaaaaga gcacggtatc agcatgtttg gcggtgtagg atgtttccct atcctccttc
    23041 aaatgccttt cttctctgct atctactttg ctgcccaaca tactgaaggg gttgctcaag
    23101 caagctacct aggcattcct ctaggttctc caagtatgat tttggttgcc tgtgctggtg
    23161 tcctttacta tcttcaatcg ctcctttcac ttcacggagt agaagatgaa atgcaaagag
    23221 aacaaatcaa gaaaatgatt tacatgagcc cactcatgat cgtcgtcttc tccctcttct
    23281 caccagctag tgtcacactt tactgggttg tcggtggttt catgatgatt ctccaacagt
    23341 ttatcgtcaa ctatatcgtt cgtccaaaac ttcgcaaaaa agtccgtgaa gaactagcca
    23401 agaacccacc aaaagcaagt gctttctcta aaccaagtgg acgaaaagac gttacccctg
    23461 aacaaccaac tgctatcaca agcaagaaaa aacacaaaaa tcgcaacgct ggaaaacaac
    23521 gttcgagata agaagaaaaa ggcttagctg atttgctaag tctttttgca agtcaaaagc
    23581 ccgatgtttc catcaggctt cttttctatc catgtacacg tttcatataa tcttgataac
    23641 tttcggtatc catgagttgt ttagcgttct tgacgcgatc tgctgttggt ggtttgactc
    23701 cttcgaggga atatggaatt ccaagttcac gccacttgaa ctcacccatg gtgtgataag
    23761 gtagaatttc aaacttatca acatttttga gggtcttgac gaacttacca agttcaatca
    23821 agtcatcatc tctgtctgtc aatcctggaa ctagcacgtg gcgaatccag acaggttttc
    23881 caatatctga tagatactgg gcacaagcca agatattttt attggtttgg ctagtgacaa
    23941 tcttgtgctg ttcttcgttg atttccttga tatccaaaag aaccaagtca gtgacagcca
    24001 tgagtttgtc aaacttctca aggtaacgtg gtttattacg gaaaggaaga gcacaggtgt
    24061 ccaaggtaca gtggattcct tgttccttag ccttggtgaa gagagcaatc aggaaatcaa
    24121 tctgcaagag agcttctcct ccactgactg taatcccacc cttatttccc cagaaaccac
    24181 ggtagcgcaa ggcctctgtc aagacatcat ctaccgtccg ttcacgtgac ttattggact
    24241 ccatagccca agtgtctggg ttgtggcaat actggcaacg catgtgacag ccctgcaaaa
    24301 agacaataaa gcgaatacca ggcccatcta ctgacccaaa gctttctgtc gaatgcacca
    24361 ttcctgtcac ttgtccataa tcaattgttt cttcagacat atcgttacct tccttgaaaa
    24421 cgttttatag ttttattata tcacgacttg aaggaaaaac aagatttttg cctatttttt
    24481 agaaagcaat ctgtttttca atttcatttt caatctatta tcagattatt cttcaaaatc
    24541 gaagccatat aaggttgcaa aatagtcctc agggcgctct gcacggcgga tttgacgggc
    24601 tttaccttct tcggtataga ggatttccgc agaacgtaat ttggcattat actggtagcc
    24661 cattgaaaat ccgtgggcac ctgtatcatg aatgaccagc aaatcaccga tttctgtatg
    24721 aggcagttcg cgattaactg caaatttatc attgttttca cagagcgaac cgaccacatc
    24781 taccacttca gctggtccat ctggatgggt cacgttgcta atatgatggt aagctccgta
    24841 catagctgga cgcatgaggt tgactgctga ggcatccaca cctagatagg tacggtaggt
    24901 ttccttctta tgagtgactc ttgtgactag agcaccgtga ggtgccagca taaaacgacc
    24961 caattcggtg aaaatcttga cctgaccaag acctgctgac gtaagaacct cttcatacac
    25021 cttacgaact ccctcaccaa tcaaggcgat atcgttcggc tcctggtctg gatgataatt
    25081 aacaccaata ccgccagaaa gattgataaa gtctagcgaa atgcccaact tttccttgat
    25141 ttcaacagcc agttcaaaga gctgacgagc caactctgga taatagagat gggtcacggt
    25201 attggacgct aggaaggagt gaatcccaaa agtcttggct cctttttcct tcaagatagc
    25261 aaaggcttca aagagctggt ccttggtcat gccaaactta gcctccccag gattgtccat
    25321 aatgtctgtc cccagttcaa aaacgcctcc aggattataa cgacaagaga tgatttctgg
    25381 aatgcctgct actctctcca gatgttcaat atcttcaaag gcatccaagt taatggtcgc
    25441 acccaattca cgtgcatagg cgtattcctt gtctggcgtg ttgttggaag agaacataat
    25501 ctcagaaccc agaaagtcca gtttatggct catcaaaagc tctacataac tagagcagtc
    25561 cacaccacaa ccttcttctt ggagaatttt caaaatagct ggagttggag tagccttaac
    25621 tgcaaaatat tccttaaagc ccttgttcca cgaaaaagct tggttgacgg ctcttgcctt
    25681 ctcacgaatc cccttctcat catacaagtg aaagggagtc gggaactcgg caacaatcgc
    25741 ttctaactct tctctattga taaatggtgt tttcataagc ttccttctat tctgtttgca
    25801 aagaaaggct gggacaatcg ttccaacctt tagttctatc tcttatttat cttcgtcaaa
    25861 gatattgcca acctcaacat cgatggcttg gttcttgaca tcaatattgg ttaccttcaa
    25921 gagtgacttg tagtcaaact gcttttcacg ttcttcttgg gcattgtccg caaagaccgc
    25981 tacacctgcc agttctgagt cgaactcgcg caagagacta atcataccat tgaccgttcc
    26041 gccacctttc aagaagtcat ccacaatcaa gacacggctg cctgccttaa gactacgttt
    26101 tgaaaggaac attttctcga tacggtcacc acttgaacct gaaacatagt tgacgctaac
    26161 agttgaacct tcggtaattt tcaggtcacg gcgcacaatg acaaaagaga cattgaggac
    26221 attggcaact gcatttgcaa gtggcacacc cttagttgct acggtcataa ccgcgtcaat
    26281 tttttggtcc ataaagcttt tggcaataat acgaccaata tttttcaaga tggctggtgt
    26341 gctaagcaaa tcagacagat agatataacc acctggcaag atacggtcac tttctgacaa
    26401 cttggtacgc aagtcttcaa ccatttcctt agcatcctga ctcgaaatag acggtgtgaa
    26461 gatgacacct ccgccagccc cagtcactgt ctggatatga ccgatttcaa tttcctcaaa
    26521 ggcgcgtttg ataatgacga tatcttctga gatggatgat ttagcagact catacttttc
    26581 agcaaaagta ttgagactag ttagtttata aggattatta atcaaatagt tggaaatgac
    26641 aaccatccga tcacttcttc ttaatttcat ttctatttcc tattccattt ttattcaaaa
    26701 aatcaaaaag caaactagaa agctggtcgc tggtggttca aaacactgtt ttgagattgt
    26761 caatagaact gacaaaccct gtaatatacc tgcatatata catacgacaa ggcgatacta
    26821 ccctagtttg aagagatttt cgaagagtat tcatttttgt cttttactta ttataccata
    26881 ttcacataaa aaaacgaaca ttcttatcct aaaaaatgct catttttctt aaattattaa
    26941 tctaaatctg gtttatagaa ggaacgatta tccatagcga agattttatt ggtcatctct
    27001 cctttatcca ccaaagcaag agctgttgac atcatcatca tgcttgcatc cagattgtca
    27061 atcatatgga taatctctgc ttccataatg cgtggacgga ctgggcttcc atactcaagc
    27121 aagccgtggt gactgaggat gacatgacga agcaaaacga cttcttcctt ggtatcatcg
    27181 atgccgagtt ccataactgt cttggtaatt tcgctatcaa tgagagcgat atgtccaaga
    27241 agattacctc gcactgtgta ctctgtctgg tctggccccg tcaactcgat gaccttagct
    27301 aagtcatgca acataatccc cgcatagagc aggctcttat tgagctgagg ataaacttcg
    27361 ctaatagcgt ctgccaaacg caccatggtc gccgtatgat aggccaagcc cgtttcaaag
    27421 gcatggtggt tggtcttggc agctggatag gagtagaatt ccttatcata cttggtgtag
    27481 agatttcgga caatccgttg ccagacagga ttttcaattt tgaaaatcat ttgcgacatg
    27541 tagtcacgaa tttccttgac atcaactggt gacttgacct tgaaatcagc tgggtcattg
    27601 ggttcaccag cttgaggcag gcggagagta atttgattga cttgaggggt attgttataa
    27661 acttctcggc gtcctttcat gtggacaacc ttacctgcgg taaaggcctc aatgttatga
    27721 ggttgggcat cccagagctt cccatcaatc tcgccactat catcttggaa ggtaaaggct
    27781 aggtagtttt tcccagctcg agtttgcctc aggtcagctg atttgattag gtaaaagcct
    27841 tcaaataact catctttttt catgtgacta atcttcatat tcttcctcat tttcttgaaa
    27901 atggagtaga tcaagcgcag gctcaccttc tgacaactca atgtgacgga gcgtccgctc
    27961 gatagctatg gtacgacggt ttaataattc atcaatattg ccagaggcat gttggagatg
    28021 tttttgtgcc ttgaccagaa taccaccaaa cttgccaaac tcggtcttga cactggcaag
    28081 agtcttgctg atatggtcgg cactcttttg gatattaagg gtcttgaaac caactgatag
    28141 ggagttaaga agggctgata gggtacttgg tcctgcaaca ataatctgtt cttcccgtct
    28201 caaatcatca aagaagaccg gattgcggac gatttctgag tagagacctt ctgtcggaac
    28261 aaacaaaact ccaaaattgg tcgtccgagg tggtgctatg tacttgttcc taatatccct
    28321 agcaaagcgc ttgacgcttg ctaggagtga cttacgacag cgttcaatct catccttgtc
    28381 acctgtctca taggcttctt ccaagcggta ataatctgcc agtggaaact tagagtcaat
    28441 tggcagatag acgtattctt ggtcgccttg tccgggtaac ttgatggcat actccactcg
    28501 ttcactagag ttttcaaccg ttgcgtattc tcgttcgtac tgggcaggtg tcatgatgtc
    28561 ttcaataatt tgccccagtt gcaattctcc cagaatccct cgcgtcttgg ttccagagag
    28621 aaccttgtta agagctccga catcacgggc aactgtctgc atttctccaa ggccacgatt
    28681 gacagactcc agttgtttag aaactgtctc aaaggaagcc tgtaagcgtg tctgcaaggt
    28741 cttttctagt ttttcctcga ccgtctggcg catttgttcc aaacgttgct catttgattc
    28801 ctgcaaggct tggagacgtt ggtctgtctt gtctctagtt tggaggagat tatctgtcat
    28861 ttcttgacgg acttgggtca gaccttggtg caattcaatc cgcacttctt gcaaacggtc
    28921 gctgacaacc acttccaaat ctttttggtc taactggctg gcttgtctgg cttggtcaaa
    28981 gcggtaatcc aactggtctg acaaatgatc tgcctgatcc tccaagctct tacttaagtg
    29041 tttctcctgc ctatcctgcc tttgccaaat cagaaagaga ccagctagat tggcaattaa
    29101 taatagaata agtaaacttt ccatcctacc tcctgtcctt gctatgcagt acgaccacat
    29161 aaccatctgg gcaagttacc gacacttccc tatctatata ttcgttagaa gcgtacactt
    29221 ttttaaagaa aaaattttcc tctgtcaact catacttggc tccaagaata gtcagctggc
    29281 tatcccgaac tggcataaag gctagatagt cgtagtctga acgaggttct agctgactga
    29341 ttccttctgg acaataagta atcaagtttt gcccatcctc aatttctatt tgatgcatat
    29401 agggtgccaa cttaggattg ctaggcagaa agacattggc caacatatgg tcaatacggc
    29461 cacccaaggc accgaaaata gtgacctgag cctgaggatt ttgttcaaag atggttaaga
    29521 gagccaattc cagatctgta tcatcctttt ctggtcgtgc ttggacaaaa tactgggcac
    29581 ctttttgaat cacctgtcgc tcttcttccg tcacagaatc aaaatctccg actgctagag
    29641 caagaggtaa gtcttcttcc aagacccaga gcgagcctcg atccacccca acaaaagcat
    29701 caaaatctgt ccgataatga ccgcggtttc cgcctgcaaa aactgcaacc ctagtccagt
    29761 tgttttctga gagtttgtac tcgctcattg acttctccct taaagacata ggaacctgct
    29821 acaaaaacag tcgcaccggc ttctttggct tgagcaatag tttggtcatc aatcccacca
    29881 tccacttcga tttcaaagtt caaacctttt tcctcacgaa gagcaaccaa ctcacggacc
    29941 ttatccatgg tttctggcag aaaggcttgc ccaccaaaac ctggattaac cgtcatgact
    30001 aaaacttggt caactagatg aaggacgtgc ttgatggctt caactgatgt gccaggattg
    30061 ataacgactg aaggcttaac tccgagtgaa cgaatttttt ggagggcgcc atgaatatga
    30121 ggcgttgctt ctacatggat actgatgatg tctgcacctg cacgcgcaaa atcttccaga
    30181 tgatgctcag ggtttgacac catcaagtgg caatcgaaaa ccatcttact atgaggacga
    30241 aggctctcga ccacacctgc accaaaactg atttgcggta caaaatgact gtccatgata
    30301 tcgatatggg catattctgc cccagttgct tctagacgtt tgatttcacg ttcaaagttg
    30361 gcataatctg ctgccagaat tgacggagca atcttgtatt gagacatagg tttctcctta
    30421 ttttggaatt tttttgctga cttttttata ggtttctcta cgattttcaa tttcactaag
    30481 gaattgcagg taattgtcaa aacggaaggt tgcaataaca ccctcttcaa cagctggttt
    30541 gacggcacaa gacggctcat gggtatgggt acaagtacgg aacttacaat ctcggctaac
    30601 agtagcaatc tctgggaaag cctgattgag gtcttcagcc cttgatactt catagtccaa
    30661 ggatgaaaat cctggtgtat ctgcgatttt acccccgttg agattgtaaa aactaacagc
    30721 tcgagtggta tggcgaccgc gacctagact gtctgaaatt tctcccgttt caagattgag
    30781 gtctggtgcg attttattga gaagagttga cttcccaaca cctgtctgcc ccataaagac
    30841 cgtaaccttg cctgttaaca aagacaggag ttcctcttta ctggtcacaa agtcatagcc
    30901 gatgtcacca taggtctgct ggtaaaaatc cagttctccc ctatcttcca acaaatccat
    30961 tttggaaata tagacaatgg gatggatgcc cttgtgctcc aaaagaacca agaaacgatc
    31021 cagcaaattg ctgttaaaat caggttcctt gacggacatg attactacag cttgatcgat
    31081 attgacaata ggcggacgaa ccagactgtt tttccgttcg tgaattttga ggatatagcc
    31141 ttctgaattt tcctcggcag agaaatctac ccagtcccca acataagggg tatggccttt
    31201 tttacggaaa ttcccacgcg cgcgtgtttg ataaacctgg ccatcactct ccacatagta
    31261 gaaacctgcc aaggctttaa tgatttgtcc ctgcatctta gagtccttgc cctttaagtg
    31321 cgtctgctag accggcaaat tctgccaagc tgagagcttc cccacgcaca cttggtgaca
    31381 agcctgcctg gtccaaagcc ttggtcagct tgtccttgac ctcttcagtc ttaccaaagt
    31441 aacctgtcaa gttattccac aaggtcttgc ggcgatgggt aaaactagcc ttggaaacct
    31501 taaagaaaaa gttctcatct tctactgcta cggctggctc tggacgacgc accattttca
    31561 agatggctga atccacattt ggcgctggca caaagaccgt acgaggcacg ataaaggcaa
    31621 ccttggctgt catgtaatac tgcacggcga tagacaagct accgtaagcc ttggtgttag
    31681 gctgggctga aatgcggtcc gctacttctt tctgcatcat gaccacaaac tcacaaaaag
    31741 gaatgccact ctcaatcaag tgcatgagaa taggcgtcgt gatgtagtaa ggcaaattag
    31801 ccactacctt gattggcagg tcaggatttt taaaattctg gatatgttgc gccaaatcaa
    31861 ccttgagaat atcttcgtta actacggtca cattatcaaa atcacgcagg gtatctgcca
    31921 aaattggcac caaacggtgg tcaatctcaa aagccatgac ttgggctgca cgctcagcca
    31981 aaaattctgt caaggcacca atacctggcc cgatttcgat gacattgacc tgatcatcaa
    32041 tttcagccgt atccacaatt ttttgaagga tattggtatc cgtcaaaaaa ttttgcccaa
    32101 aggacttttt aaaggtaaaa ccgtgacgct ccagcactgc cttggtcacg ctataatctg
    32161 caattctcat ctattctctt ttctattatc tgttactact attgtaacac attccccttg
    32221 tattttggga atgaaaatca taccaaaaaa ctatctttcc tcatgaagaa agatagtctt
    32281 ggaaatcttt aaaagtggcc ttagcagttc tctcgaaaca tgcgttcaaa aaaatcagct
    32341 tcttgataac ttcgattttc aggtagggtc aaatccacat cctcctccac atcatccact
    32401 gtcagttcaa ccgtctgagg taaatttttc aaacgataat gcttttgcct ttgcccttca
    32461 atactagcaa aatcaagcaa atagccatgc actcggattt gaaccaaaag gagatgattg
    32521 gtcatatttt ccatgatgag gatatcttct aaaccacctc tggcactagg ataatcagat
    32581 accctgcgaa tacggatttt ttcacacatg agcaagtttc ctcatatcaa tgatttcatc
    32641 agccttattc cagactaggg gattatgggt cgcaatgatg ataattcgat tttcatcttt
    32701 caaatccacc aagagattca taacctcctc tgaattttca ggatcaagag ctgctgttgg
    32761 ttcatctgcc aaaatcaagg gtggattttt caaaatagtc ttagcaaggg caactcgttg
    32821 ggcctctccc ccagataaag tatagatttt ttgttctaaa tccaaatacc ctagattaac
    32881 tttttctaaa gcccccactt gcctttccaa acgttctact tttgagattt tctgaccaac
    32941 aaaacccaaa tccaaatttt ctttgattga ttggttttct aagaggccga aattttgaaa
    33001 gagatagccc atctggtctc gaaaatactc acgagtggga atggttttta aatctttccc
    33061 ctgatagaga acccttccac catctatctt ttctagcttt cccaagatat tcagcagcgt
    33121 cgtctttccg cttccactct ttccgattaa ggcataaacc ttgcccttct ccagcttgag
    33181 attcaaacca gagaaaatcg cgcggtcatt aaatttcttt tccaatcctt gaatatcaat
    33241 catcttattt tcctttcaat actgccatgc taccagcttc ttcttttttg tcctgcctta
    33301 ctaaaatcaa gagggcgtta agcgtaaaca aagctacaac tagagcgcta atcaaaccat
    33361 ctcttgtcaa aatactagat aggactaggc caagcaagag aactcctcct tgcgccagta
    33421 aatacttgcc atgaagctca tagattgtca taccagcaag acgtttaatc ataagttccc
    33481 gtctgaactg ctcaaaatag agaagattca tggaatcaaa taacaagata gccgtagaca
    33541 gggtcaaaat cgtcccaata aggagagagt aaaactccac ccgtttgtca tttagtacct
    33601 tggcaaaaaa tagctggctt tttaccaagt aagaaacctt gtgatacaga cctttctctt
    33661 ttagagctgt gatgctgtct ccatatcgat ctagtttcaa ggcactatta gccgtagttc
    33721 cccacaacat ccctgcaaca ggtcttgttc cagtcgcttg cggcgttaga actacaataa
    33781 tcggatcttt taggtactgg cgtgttgtct tgtacccatc attatagagg aaacgttcct
    33841 gtcctgtttc tgtaaaagct agccttacct gtgggaggta gtgtttctga ctcgtcactt
    33901 ctacactttc agatgaaaag ttttgcaggt aatctgtaaa caatccttgg tagtagacag
    33961 actgctctcg caagctctca ggcaagatca gcccaaactc tccctcagac aagttttgca
    34021 tcttgtccat aaactctgaa gaaacggtaa tcttttcttc tatcagatag cgcggtgaga
    34081 catagataac attccctgac ggtgtgtagt cactgagacg attgccatct aggtccactt
    34141 ctgctccatc tgagaaattg tcaacattgc tcataatata aaaagagtct gtattggcta
    34201 accgttcttc agtaaatgtc tgccactcac gattatcctt acgcgttcct tcttcatcgg
    34261 caaatgcact agaccaacca aaggatagac ggtaacggtc tgaggactgg ctccatttat
    34321 tgctagctct ctccatttcc tgcatttcac ggtagtgggg taggagagct gtcgcactcg
    34381 atccgaccac caatacagct aagagttgcc ccaccatcat caatgtcatc atacgtttga
    34441 gagggagttt ccctttcaat agatccacca gactattttc ctgtaaacca agtagataga
    34501 cgacacttag taaggtagaa atccctgcca aggtcaatcc ataaagtaga agagcaatga
    34561 tgaccagttg caccgttgcc ataaacaagg caccttgata ccagagaatc cccaatccca
    34621 aaagactgga taccagcact gagcagataa gctgtctcac atcttctaac actggtctga
    34681 gagcaactcc aaacaagctc tcaccagcta ttaagcgaat ccctgcctga cgaagggatt
    34741 tgatccgata aatcagggtc agactcataa aggtcagcag aaaaatagct aaactcagta
    34801 gcaccatagg ggtggccgtc aatagtaaga ctatcgaaaa tggatcttct ccattcgaaa
    34861 caaagccttg ataaccaagc tctttcaagg tggtctgtaa gctcactcca tccaaacttc
    34921 ctgatacaat caagtaagac cctaataaat cactcgtttc tgcactctcc ttggaggaaa
    34981 tggtcagacc ttctggaagc tttccctcac cataagtggc ataggtaaag cgtgtttctc
    35041 cagcttcatt tggctcaacg attcgacgag caatgagact attgtgctct accgccaagt
    35101 gactcaaagc ctctgtcacc tcttcgcgac tagctactgg ttgatttatc atcattcctg
    35161 tcacatcaac agaaggatag ttaccaaata aaatctcttc tcgatggtca acggaagtta
    35221 tcaccatata aagcgatact aataccattg aaatcaaaat aaataaacgt ttcatcattt
    35281 cctccaaaaa gttacttggt cacttcgtga gaaggtgacc acagtcactg cgatgttaag
    35341 aagcttatta agttctttct gctagcaaca tcttcaaata cattatatcc gttttttcaa
    35401 gaactatcaa ggacatcaat gtccttaaaa tctaaccatt tcgtgtcatt tttagcttat
    35461 atctaaaaaa acatccctaa agacgaactt tagaactgtt ttgatacgag gacaaggaca
    35521 tttttgtcac tgattcacta tgtttataaa tattttttga catcctcttg ccattcctct
    35581 gtcaatttct ttactagaaa ttcatctcca attagctgcg caaatgtttt agaagactga
    35641 taaaatgatt cagctaaact aaaatctctt tcttctctta atgcttgctt ccacaaaaac
    35701 ataaatacaa gaggtttctt ttgataatct tgagttttat ccataatttc ttgtaaacac
    35761 gatagataat cattaaaaca atcagttact cctacctttt caagacaaca cattcctgaa
    35821 ataagagtat ctctaattat aaagtaattc tctatttgag ttaccttctt ctgttccaaa
    35881 agaaagagca ttagcttttc gatttgattt ataaatttgg caagatcttt tctaatgagc
    35941 atttgataaa aaaacaaacg aatcaacaac atatcattta ttgaataagc ttctttttct
    36001 ataatctgag gcatatgttc ctcaagtatt aactctgcat attcaggcct tctagttcta
    36061 gaagtatcca aacacgcctg caatacttca catgctaatt gttcttcttt aggaagatta
    36121 tcaaagtatg ttttataaat ctcttccaaa cacgcttcct tcttatcgta ctcctcttct
    36181 ttaccataga ttggttcacg taaaatctgg tatttcaatt ctaaataagc agaaggaaga
    36241 gctgaaaaat ccggcataag gctataaact ggaactccta gccggcgagc aatatagtct
    36301 aacttgggca aactcggttg cgaagctcca ctttcaattc gcgacagttg acgaactgtc
    36361 agttcttgct catctccaca aaaatctgga cgactaattc ctttttctat ccgaatctct
    36421 ttaatttttt gaccaattaa catcagacgc ctcctcttct acctgcttcg aaaaatactg
    36481 tgaaacttaa aaaattgtaa cggctacatc tttcctactc ataagatttc atagcttctt
    36541 ccacttctgc caaagtaacc ccaaacaact ctaggcgttt gaggagttgc ttgccgttgg
    36601 aatagccgat tcggagagtc tctccgagat attctctacg cttacggctg tctgcccctg
    36661 ctagaaaacc aaggcgaatc aaatcgctac gactaatgtc aaactgactc tcatgttcaa
    36721 attgttctgt cacctgagct agagccgttt tcaggtcttc atagctggca tgctcaattc
    36781 ccagagaacg ccccttggtc ttggacttgg gaacagcttc atctcgcttg agaaaggcat
    36841 gctgaactgt tggaatgacc atcatgatca tgcgccgaat ccgttcccca ttaaaatctg
    36901 ggtctgtaaa gacaatgact ccatgacgtt ggtgcaggcg ctgaatccgc tctatatcct
    36961 gagcattgat ggcagaacct cgagtctcat aggtctccac atcgaaataa cgtttgagat
    37021 tgaccgtatc atcacgccct tcaaccacga taacttgaga aattttctct ttcattactt
    37081 gctgtccagt ccaaaaattc gttctgcatt tgcagtcgtt gctaccgcca gctcttctgt
    37141 cgtcatacca cgcaagtcag cgataaagtc gaccacatag cgagtatagg ctgttttatt
    37201 ttcacgacca cgcttgggta caggtgctaa gtaaggcgca tctgtttcca ccaacatctt
    37261 gtccaaaggt aactctttag ctgcttcttg gaggtcagtt gccttcttaa aagtcaccac
    37321 tcctgagaag gaaatggtca taccaagatc cacaaacttc tctgcccact caagcgtccc
    37381 tgaaaatgaa tgcatgatac caccacgagg accaacgccc tcactcttga taatctcata
    37441 ggtatcttcc agcgcatcac gggtatggac aacaaaaggc aaatccaagt ccttagatag
    37501 ctgaatctga cggcgaaaaa cctgctcctg cacctctttg ggcgctgtca tccaatggta
    37561 atctaagcca atttcaccta aagccacaac cttggaatgt tttaacttat ccaacaagta
    37621 agcctcaact tcctctgtat aagtaccagc ttctgtagga tgccaaccaa tagtcgcata
    37681 gagctgctca tactcatcta ccaactccaa ggcatgctca atcgtcggtt tatcaaaacc
    37741 aacaatattc atctgtgtca cacccatctc agcagccaag gcaatttctt ctgcctcacg
    37801 acctgcaaat tcttctacat tcaagtgtgt atgtgtatca aaaatcatct cttctaacct
    37861 cgttttctat cttctattat accaaaaaag aggaggggca cctaattttt cggtttcccc
    37921 tcctctcttc aatagagagc tattctgcta tcttttctat ccgatattgc ccatctccta
    37981 ttccacagtt agagacagaa gagattggct acattgatag aatattccta ttcagctaaa
    38041 gtatgtgatt atcttgggtt ctcacttttc tcacccatca ctaggattga tgacttctca
    38101 aaatccccct tataatctaa ctttaagtag agaatagaat tttctagctg ttctattttc
    38161 tacgtctagt ctttcttcct agcaaagcaa aaccacttgt tgccaccact agaccaagac
    38221 tagcaagtgc tgcacttgat gctgaacctg tgttaggtaa ttgtttgcca gcaggtgact
    38281 ggtaggtaac atcttgcatt gttcctgcag ttgcttgcgg tgcacttgtt gtcttaggag
    38341 cagcattagg agccactggt ctcttaggaa ctgtaaccat ggcaatggct tgcactccaa
    38401 tcgtttctgc ttccttcaca tcttccatag ttttcgcatt ttcaatctct ttgagagctg
    38461 cttgtttttc tgcttccact cttgctaaaa gttctgcttt ttctttatca gaaagcggtg
    38521 cgcctttgat ttcatcctgc ttgtccttgg ctgctttttc aatggcattt ttagctgatt
    38581 ctttttcagt agctaattgc ttagaagctt gtgattgagc tgagttttgt gaacctgtgt
    38641 gagctgatcc acccgcctgg ctaccaccgc cttcattcgc attagcatct gagcttccag
    38701 tattgcctcc cttatctcca cctggtgtat tttctagctt atactctggt gtagcttctt
    38761 gagttacaga ttcacctttc gcagattgtt gaactgtatc ttttcctaga atcgtcacta
    38821 ctgaaccatc tgggaatgtg attgttgctg taccatctcc agctacattg attgtcgctc
    38881 cgtctaatgc tgaaccattt gcttgtaaaa tagcaacctt aactttttct tcatctgtta
    38941 aatgagttat atcttttaca tctactcttt gagcaggtgc tttaacagtt gatttttcag
    39001 cagtcagttt agtgctatct tgtgcttttg gatcttgtgc taccaattga tttcctggaa
    39061 ttgtaatagc tgaaccatct ttaaagacga ttgttgctgt accatctcca cctacttcaa
    39121 ctttatagta aaataaaata ataacaggac aaatcgatca ggacagtcaa atcgatttct
    39181 aacaatgttt tagaaacaga ggtgtactat tctagtttca atctactgta acatcgcttc
    39241 cgaatccatt tgcatctact gaactttttt acaaaaataa aaatcagtag actcgagttc
    39301 ctactgattt ttgatagaat tcgtttggat tatgcagcca aaactttgcg tcctttacga
    39361 cgacgagctg ccaatacgcg acgaccattt ttagttgaca tacggttacg gaatccgtgt
    39421 ttgcgcgcac gacgaagttt acttggttga taagtacgtt tcacgatgaa tacctcctca
    39481 tagattttgt attcgtttag ccggctataa agtgatcagt tactaaacat actttactat
    39541 tctatctgat tctttcactt ttgtcaatag ctctaggcaa atgtttccag ctttttcgaa
    39601 tgcaagccct atctacgata ctgtttcaag aaacgagtca tcttttcttg gatttgggct
    39661 aactcatcaa cacgaggaag gtaaacgata cggaagtggt ctggttcctg ccagttaaag
    39721 cctcgaccat gaaccaagag aaccttttcc tgcttcaaga aatcaaggac aaactgctca
    39781 tcatcatcga tacggtacat attgcggtcg atttttggga agatatagag ccccgccttg
    39841 ggtttaacgg cagacaaacc tggaatatct tgaatggcat tatagatgaa atttctttgc
    39901 tcgtagattc gtccaccagg aagaagcaat tcatcgactg attggtgacc ccccaaggaa
    39961 gtttgtacga cttgttgggc caaaacgtta gagcaaaggc gcatattgga cagcatattg
    40021 agcccttcga tatagccctt aacatgagtc ttagggccag acaagaccat ccaacccaca
    40081 cggaaacctg ctatgcggtg ggattttgac agaccattca tgctgacaca gaagacatct
    40141 ggtgccaagc tcgccacagg cgtatgcaca tgtccgtcca ttaccatgcg gtcatagatt
    40201 tcatccgcaa agataatcaa atcgttttga cgggcaatct caataatctc cagcaagagt
    40261 tccttaggat aaagggctcc agttgggtta tttggattga taaggacaat tgccttggta
    40321 ttggaagtga tttttgactt gatatcgtca atatctgggt accattccac agcttcatca
    40381 cagatatagt gaacggcatt tcctccagct aggctgacag ccgccgtcca tagaggatag
    40441 tctggcattg gcactaagac ctcatcccca ttgtccaaaa gcccctgcat ggacatgaca
    40501 atcaactcac tgacaccatt tccaaggtaa atatcatcaa tatctacgtt tgggaatttc
    40561 ttcagctggc aatactgcat gatggcctta cgggctgaga aaatcccttt ggagtcagaa
    40621 tagccttcac tatcacgcgc attcataatc aagtcatgaa tgacctcgtc tggcgctgta
    40681 aagccaaatt ctgctggatt tcctgtattc agacgtaaaa tcttttctcc attcgctcgc
    40741 atccgcatgg cttcttccaa tacaggacca cggatatcat aagcaacatg ctctaactta
    40801 ctagacttgt tatattcttt catcttgttt cctcaattca tcaggatagt aacattatac
    40861 cactagaaag agaatgcgac aagtttactg aaatgtgtta ctagtttcgt atagaagaaa
    40921 aaccttgcta aaggcgaggt taagaacgta gagatagcag gtattttgca aagacacatc
    40981 acataccaaa agataaacct agaaaaaagc aagcctagaa aggagtcata aagaagatta
    41041 aacatgagga aaagccccca tatcatcaag taatcccaac gcccacttcg ttttgcaact
    41101 agaaataaga ttagatggta gagtacatga aaaactaaaa atccaataaa acttggataa
    41161 agaagcggta ccagactctc taattgacag atcatgatca cttctaccaa gactgaaact
    41221 aggataattc cataataaag gaaatttgat ttttgaaaag agtttttcat cattctccct
    41281 tccttcactt ctttcctgct atcctttctt ttattttatc atatatttat atagagttta
    41341 agccctaata ttcttttgaa aaatactttt tttgctttac ttctatagtg aaaaggttta
    41401 caattatagt aagaaaattt caggaggttt cattatggca caacgttacc aaaatatcat
    41461 ggtcgcaatc gatggttcta aggaagcgga cttggctttt gtcaagggag ttcattctgc
    41521 tctacgaaac gacgctaaac tcaccatcgc acatgtcatt gacacacgcg ctctccaaag
    41581 cgtatccacc tttgatgctg aagtttacga agaactccaa gtcgacgctg aaagtctgat
    41641 gaaagagtac gaaaaacgtg ctaaagatgc tggggtagca gatgttcata tcgtcattga
    41701 aatgggaaat ccaaagaccc tgctagcacg tactattcca gatgccgagg aagtggacct
    41761 catcctcgtt ggcgcaactg gtctcaacgc ctttgaacgc ctcttggtcg gctcttcatc
    41821 tgaatacata ctccgccatg ctaaggtcga tttgctggtt gtgagagaac aagaaaaaac
    41881 cttataatca caaagaaaag gagcccctag ctcctttttg tttacgattt atttctctct
    41941 ttatggcgtt cgtaagcctt gagctggcgc tgcagttcct ttttaatagc aggttctgga
    42001 gcatattttt cttcccaatt atctggtttt aagattttat gggtcactgg atcaaaatga
    42061 gccttgccat ctggaaaaat tttccccata ttggcctgat ggacaatatc aaaaatacgt
    42121 tctgggtcca cccccatcaa gacaaaactg ccgtaggtga agtaaagcgt gtcaatcaag
    42181 gcatccactt gccctatcaa atcttgctga gcaggtgtct tcttggctac tttatctgct
    42241 gccttatcaa gggcctgatg aagttgcgat acagcttgac caaaatcttc ttcagaagga
    42301 ctggctgctc gaacaaactc caccaattct tctattttaa aaccagccct atgggttgca
    42361 ccctctaaat cccaagctcg aggttcttct tgggttcgtt catccatcat gtggtggaag
    42421 gtcttgacct tattgaaatg atagtcacgg ctgacaaaga ctttttctga agacaaaaca
    42481 ccaaaatgtt ccaaggcctt gtggattcca tcttgttgat tgctagtggt aatgtgcttg
    42541 gcagcttcct tgacactgct actaccattt cccatagcga ccgacatacc aacgccagcc
    42601 agcatttcaa gatcattatc tgagtcacca aaagccatga cttggttgag gtcaaagcca
    42661 tattctttcc caactcggcg aatgccttct aatttagaat ttccctgatt gatgacatcc
    42721 gatgcaaaag gattgctacg tgtcaatttc aagtcttcaa aatcagctgc cgccttctca
    42781 gattcttctg gtgtcatcag catcaaaact tggtagatag gctgattcat caggtgaagc
    42841 aggtcctctt ccttttgggg aacaaccttg ctgaccatgc gattaaaaga ctgactcacc
    42901 gtccgagtta aaacagaggg aacgaagcga ctaattcgtt gggaaaaaga acccagacca
    42961 aaggacatga ttttagaacc caacatggca tccttggtcc ctagagcaat ctccgtgccc
    43021 tcttttttag catagctaat tagatggcgc aaatgtaact tggaaatagg gctcgtgaac
    43081 aagactctgt ctttactaaa gatatactgg ccattatagg ttaccgcaaa atccagatcc
    43141 aaatcgtcca tcaattcctt aacaaaaaaa ggtcctcgcc ctgtcgctac gccaactagt
    43201 accccttgtt ctttgacaat cttaatcgca tccttagtgg atttcaaaac actcttgcga
    43261 ttgttgacca aggttccatc gatatcaaaa aaaacagctt tgacttccat cctatcccaa
    43321 tctccccttt tgtgatacaa tgattatacc acatttcaga aagagtgagt aaatcatgcc
    43381 taagaaaatc cttgttttac atacgggtgg aactatttcc atgcaggccg atgcttctgg
    43441 cgctgttgtg acgagttcag ataatcccat gaaccatgtg tccaacccac ttgaaggaat
    43501 ccaagtccac gccttggact tttttaacct tccaagtccc catatcaaac ccaaacatat
    43561 gctggtcctc taccagaaaa ttaaagagga agcagataac tacgatggag tggtgatcac
    43621 acacggaacc gatactttag aggaaacagc ctatttcctt gataccatgg aagttcccca
    43681 tatgcctatc gttctaacag gagccatgcg tagctccaat gagctcggta gtgatggtgt
    43741 ttataattac ctaagtgctt tacgagtggc cagcgatgac agggctgctg acaaaggagt
    43801 tttggtcgtt atgaacgatg aaatccacgc tgccaagtat gtcaccaaaa cacatacgac
    43861 taatgtcagc accttccaga ctccaacaca tggccccctt ggtctcatca tgaaacagga
    43921 aatcctctac ttcaaaacag ctgaacctcg tgttcgcttt gaccttgatc acatacaagg
    43981 tttagtccct atcatctcgg cttatgctgg tatgacagat gagctgattg atatgctgga
    44041 tttagaacac ttggacggtt tgattatcca agccttcgga gctggtaata ttcccaaaga
    44101 aacggctcaa aaattagaaa gccttctgca aaaaggaatt ccagtcgctc tggtatcacg
    44161 atgctttaac ggtattgccg agcctgttta tgcataccag ggtgggggcg tacagttgca
    44221 aaaagcaggc gttttctttg ttaaagaact caacgcccaa aaagctcgct tgaaactcct
    44281 catcgccctc aatgccggac taacaggaca ggctttgaaa gactatatgg aaggctaata
    44341 ctcttcgaaa atctctgcaa accacgtcac gtcgccttac cgtatgtatg gttactgact
    44401 tcgtcagttt
//
LOCUS       DAVHEX010000008        41231 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVHEX010000008 DAVHEX010000000
VERSION     DAVHEX010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 41231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41231
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.008"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            82..1842
                     /locus_tag="VVL45_000458"
     CDS             82..1842
                     /locus_tag="VVL45_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HEU4186689.1"
                     /translation="MSIDEYVIGKGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIY
                     WNKKTNKYKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIG
                     RSAMVTKLLCIYSEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVSKTYP
                     ELEPSKLGTMLFEYSKLFMENKEDNSTMDSSNNFRHQLTQSLLKSPNLILRGAPGTGK
                     TYLAKEIAKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKD
                     FCQKAKETQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSINSRQNLSVNYDSG
                     VSGWSLPSKYVYELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKKF
                     VFIIDEINRGEISKIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVYIIG
                     TMNDIDRSVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIEN
                     VQELNSHYHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAF
                     ELTNNDQTGQQDTGDNDADY"
     gene            1829..3145
                     /locus_tag="VVL45_000459"
     CDS             1829..3145
                     /locus_tag="VVL45_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HEU4186690.1"
                     /translation="MRITDNQYKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLHINLTSLDVALSREERLYQLLMYLFPKYLQAAIRKGLYKEYQRFSHNDSHVKGV
                     IDVRNHLKKNLPFTGNIAYTTREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKIIRGNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLVFPSKDKVIDNEMGNL
                     AGYGALLKKWSIQIPQKSSSYSAFCKMMENSEENFKAIIDEEVGRK"
     gene            3320..4306
                     /locus_tag="VVL45_000460"
     CDS             3320..4306
                     /locus_tag="VVL45_000460"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEU4186691.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHDQGLIASISVGVKDYEY
                     DFVRQLKTDAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            4370..4441
                     /locus_tag="VVL45_000461"
     tRNA            4370..4441
                     /locus_tag="VVL45_000461"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4403..4405,aa:Arg,seq:ccg)
     gene            4624..5322
                     /locus_tag="VVL45_000462"
     CDS             4624..5322
                     /locus_tag="VVL45_000462"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEU4186692.1"
                     /translation="MKELQTVLKNHFAIEFTDKNLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTYLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            5313..8852
                     /gene="smc"
                     /locus_tag="VVL45_000463"
     CDS             5313..8852
                     /gene="smc"
                     /locus_tag="VVL45_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEU4186693.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKSEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELELTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAALEDKRNS
                     LSKEKYDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLAIAKEKASQQKEELETAKEQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDNLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDVYYQTALEIALGASSQHIIVEDEESATKAIDFLKR
                     NRAGRATFLPLTTIKARTISSQNQDAIAVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHARAAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAEEEASLGSEEATLKTLQDEMARLTESLEAIKSQGEQARIQEQGLFLAYQQTSQQV
                     EELETLWKLQEEEIDRLSEGDWQADKENCQERLAAISSDKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVADIERLGKELDNLDFEQEEIQRLLQEKIDN
                     LEKVDTELLSQQAEESKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQSQLTDQYQISYTEAIEKAHELENLNLAEQEVKDLEK
                     AIRSLGPVNLEAIDQYEEVHNRLDFLDSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            8849..9643
                     /locus_tag="VVL45_000464"
     CDS             8849..9643
                     /locus_tag="VVL45_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU4186694.1"
                     /translation="MTIKLVATDMDGTFLDENGRFDMDRLKSLLVSYKEKGIYFAVAS
                     GRGFLSLEKLFAGVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYI
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGEAWVNDNVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKDQSVIAYMEGL"
     gene            9643..10461
                     /locus_tag="VVL45_000465"
     CDS             9643..10461
                     /locus_tag="VVL45_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU4186695.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKATLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGQEDEYTITFNGGLVQKNTGEILDKTVFSYADVARLYEKTE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGINQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAAANVVMPMTNDEEAVAWAIEEYVLKEN"
     gene            10465..11754
                     /gene="ftsY"
                     /locus_tag="VVL45_000466"
     CDS             10465..11754
                     /gene="ftsY"
                     /locus_tag="VVL45_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEU4186696.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVP
                     QEEAEVEIVEQAVFQEEEIQDTVEESQDSEPAVGVSQEEIEELPNSEELPEEEKLEHE
                     GTVEENNSEVLEPERPQTEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEE
                     LEELLIMSDVGVQVASNLTEELRYEAKLENAKKPDALRRVIIEKLVELYEKDGSYDES
                     IHFQDNLTVMLFVGVNGVGKTTSIGKLAHRYKRTGKKVMLVAADTFRAGAVAQLAEWG
                     RRVDVPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGR
                     IIKRVVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAI
                     REELNIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(11798..13285)
                     /locus_tag="VVL45_000467"
     CDS             complement(11798..13285)
                     /locus_tag="VVL45_000467"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEU4186697.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYKSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPID
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRGVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVGASWKLIDRIEELWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFDAKWTWQPDIAYRQDGRFE"
     gene            complement(13434..14174)
                     /locus_tag="VVL45_000468"
     CDS             complement(13434..14174)
                     /locus_tag="VVL45_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU4186698.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            complement(14174..16339)
                     /locus_tag="VVL45_000469"
     CDS             complement(14174..16339)
                     /locus_tag="VVL45_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEU4186699.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALSEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            16509..17462
                     /locus_tag="VVL45_000470"
     CDS             16509..17462
                     /locus_tag="VVL45_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU4186700.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAFTLYGIG
                     LLALIGLARNFGEAGQNLASYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            17539..19527
                     /gene="uvrB"
                     /locus_tag="VVL45_000471"
     CDS             17539..19527
                     /gene="uvrB"
                     /locus_tag="VVL45_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEU4186701.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAIAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTIGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            19537..20100
                     /locus_tag="VVL45_000472"
     CDS             19537..20100
                     /locus_tag="VVL45_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4186702.1"
                     /translation="MIYLRKLKKEDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKETRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNIGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            20066..20554
                     /locus_tag="VVL45_000473"
     CDS             20066..20554
                     /locus_tag="VVL45_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4186703.1"
                     /translation="MTVIIKSMETPEEIEGKSFVHWQTWREAYDDLLPAEFQETMTLE
                     RCRLFSQKYPENTLIAMDGVKIVGFISYGNCRDETIQAGEIIALYVLKDYYGKGIAQK
                     LVKAALTDLNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIKEKRMVFY
                     SK"
     gene            20623..21090
                     /locus_tag="VVL45_000474"
     CDS             20623..21090
                     /locus_tag="VVL45_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU4186704.1"
                     /translation="MSRSQLTILTNICLIEDLETQRVVMQYRSPETNRWSGYAFPGGH
                     VENGESFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVVCYKATEFSGTLQS
                     SEEGEVSWVQKDQIPNLDLAYDMLPLMEMMDAPDKSEFFYRHRTEDDWEKKIF"
     gene            complement(21087..21602)
                     /locus_tag="VVL45_000475"
     CDS             complement(21087..21602)
                     /locus_tag="VVL45_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEU4186705.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQRLAEHFHVTRQVIVQDIAILR
                     ADGSPILSTNRGYIYKDANANTYHHKLFKVKHEVEEIGRELLAIVDNGGRVQNILIDH
                     PVYGEIETLLKLTCRRDVQHFLEQVERSDFKPISELTDGIHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(21614..22141)
                     /locus_tag="VVL45_000476"
     CDS             complement(21614..22141)
                     /locus_tag="VVL45_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU4186706.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWIFNFVLAVVHALAEVLACVVFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            complement(22423..22986)
                     /locus_tag="VVL45_000477"
     CDS             complement(22423..22986)
                     /locus_tag="VVL45_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU4186707.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            complement(22970..23521)
                     /gene="coaC"
                     /locus_tag="VVL45_000478"
     CDS             complement(22970..23521)
                     /gene="coaC"
                     /locus_tag="VVL45_000478"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEU4186708.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            complement(23533..24222)
                     /gene="coaB"
                     /locus_tag="VVL45_000479"
     CDS             complement(23533..24222)
                     /gene="coaB"
                     /locus_tag="VVL45_000479"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEU4186709.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGYEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISADQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            24452..26122
                     /locus_tag="VVL45_000480"
     CDS             24452..26122
                     /locus_tag="VVL45_000480"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEU4186710.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELGIDQRR
                     ILWKRVVDLNDRTLRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAAL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNDLSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            26450..27625
                     /gene="mutY"
                     /locus_tag="VVL45_000481"
     CDS             26450..27625
                     /gene="mutY"
                     /locus_tag="VVL45_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEU4186711.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQVYAQANLDSSQD"
     gene            27681..28385
                     /locus_tag="VVL45_000482"
     CDS             27681..28385
                     /locus_tag="VVL45_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4186712.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            28378..29727
                     /gene="vicK"
                     /locus_tag="VVL45_000483"
     CDS             28378..29727
                     /gene="vicK"
                     /locus_tag="VVL45_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEU4186713.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQV
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            29729..30538
                     /locus_tag="VVL45_000484"
     CDS             29729..30538
                     /locus_tag="VVL45_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU4186714.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            30629..30871
                     /locus_tag="VVL45_000485"
     CDS             30629..30871
                     /locus_tag="VVL45_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEU4186715.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            30873..31127
                     /locus_tag="VVL45_000486"
     CDS             30873..31127
                     /locus_tag="VVL45_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU4186716.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            31202..33073
                     /locus_tag="VVL45_000487"
     CDS             31202..33073
                     /locus_tag="VVL45_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HEU4186717.1"
                     /translation="MTIDISEESLAKESADLLKILLKDRTTKKSIVWATHSYELLGKG
                     FAPSDRINPSKVTGNFANLIQPRSEKSKYEQKDRTKIRAEVFTPTWLVAKQNGYVESK
                     LGSLSLEEYVDLRWLEVTCGEAPYMVTRYDTVTGEEIPLSERVGFVDRKLQRISREVS
                     DEVTFYKLVKDAYHASYGYEYQGDSLLLARENLLATFEDYYLAKTGNQPTLEQKKEIA
                     TIISYNVFQMDGLKKSSPYSAKQKQSQQLSLFADELEVQEVEESKTHIKDWKKNRMIG
                     FERLSSEESEMKFDVVIGNPPYQETGEARDEPIYHYFIDEAYKIADKSILITPARFLF
                     KAGQTPKNWMEKMLEDKHLKVQFYELKSGKVFTGTDIKGGVAITYRDVDKDLGPIGVF
                     TIFETLNGIVKKIEKHPEFETFSDLVYPQGIYRFSNKLFSDYPNAEKLQGKGTKNKIV
                     SKSLTQLDFVFKNNEEKDHVALLGRIGSERVYRYINKKYLDLPETFENYNVFVPEANG
                     SGALGEVLSTPLIGEPLIGHTDTFLSIGNFKTKFEADACIKFIKTKFARVLLGVLKVT
                     QHNSRKTWYYVPLQDFTVNSDIDWTQSVTDIDRQLDQKYDLSPEEIAFIENHVREMD"
     gene            33238..36492
                     /locus_tag="VVL45_000488"
     CDS             33238..36492
                     /locus_tag="VVL45_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU4186718.1"
                     /translation="MPVEIKTTKEIHPKIYAYTTPTVTSNEGWIKIGYTERDVTQRIK
                     EQTHTAHIATDVLWTGDAAYTEEPDKGKTFKDHDFHHFLSFHDVERRPKTEWFYFNGT
                     PEKSKNLFDKFVQHDLSGYQPGKGQDYTLRQEQEEAVAKTLAYFQEHAGGKFLWNAKP
                     RFGKTLSTYDLARRMEAVNVLIVTNRPAIANSWYDDFETFIAGQTTYKFVSESDSLKS
                     RPILSRQEFLGILADDVRQLAFISLQDLKGSVYLGGEHDKLKWVTDLHWDLLVIDEAH
                     EGVDTFKTDQAFNKIRRNFTLHLSGTSFKALAKGDFTEEQIYNWSYADEQAAKYSWSL
                     EQEEENPYESLPQLNLFTYQMSQMIGEKLEKGAQIDGENIDYVFDLSEFFATDDKGKF
                     IHEHDVRNWLDTLSSNEKYPFSTKELRNELKHTFWLLERVASAKALKALLEEHPIYEN
                     YEIVLAAGDGRMSEEDDKVKLKSLDLVRKAIAENDKTITLSVGQLTTGVTIPEWTGVL
                     MLSNLKSPALYMQAAFRAQNPYSWSDNKGNHFRKERAYVFDFAPERTLILFDEFANNL
                     SLVTAAGRGTSATREENIRELLNFFPIIAEDRAGKMVEIDAKAVLTTPRQIKAREVLK
                     RGFMSNLLFDNISGIFQASQTVLDILNELPVEKEGKVQDSSDLLDFSDVTVDDEGNAV
                     VDHEIVVNQQMRLFGEKVYGLGESVAELVTKDEERTQKQLVNDLSKTVSSVIVEELKA
                     DYSLKTRETEQIKKQITATLENEIRKNDIERKISEAHIKQELQQQLKEANDKAQKDKI
                     QEDLEKRLEENKLIHKEKLEQTLKKEVEKMPEKFIEQVEIKRVEQLKQSAQDEIRDHL
                     RGFARTIPSFIMAYGDQTLTLDNFDAFVPEHVFYEVTGITIDQFRYLRDGGQDFAGHL
                     FDKATFDEAIQEFLRKKKELADYFKDQKEDIFDYIPPQKTNQIFTPKRVVKRMVDDLE
                     KENPGIFDDPSKTFIDLYMKSGLYIAELVKRLYNSNGLKEAFPNPEERLKHILEKQVY
                     GFAPSEIIYNISTNFIFGNLSKDISRKNFVLADTIPAAKEGSIQKLVDSYFENN"
     gene            complement(36802..37788)
                     /locus_tag="VVL45_000489"
     CDS             complement(36802..37788)
                     /locus_tag="VVL45_000489"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEU4186719.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            37982..40450
                     /gene="gyrA"
                     /locus_tag="VVL45_000490"
     CDS             37982..40450
                     /gene="gyrA"
                     /locus_tag="VVL45_000490"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEU4186720.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMIGQVLSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            40450..41193
                     /gene="srtA"
                     /locus_tag="VVL45_000491"
     CDS             40450..41193
                     /gene="srtA"
                     /locus_tag="VVL45_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HEU4186721.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSNEILTAFNQPYKQFY"
BASE COUNT        12865 a         7240 c         9087 g        12039 t
ORIGIN      
        1 aaggcaactg ttggtgaaaa gtttaatgat aaattaaaag aagagtttct ccaggaatgg
       61 cctttggata ggattttaac aatgtcaatc gatgaatatg taataggaaa gggacagcaa
      121 aataagtctt tatgctacgc tcttgagaag ggaaaataca aaaatctatt tcttggaatt
      181 tctggtggct cagcttcaaa atttggtatt tattggaata aaaaaacaaa caaatataaa
      241 gatcaagcta ataatgagat ttcagagttg gatcagcgat tttcaaaatt aaaatcagat
      301 ttgtatgaaa ttatcaaaga aggtattcgt tttaactttg aaaatcctat ttttgatatg
      361 aaaagatcaa caaatgaatt tattggtcgt tctgctatgg tgacaaaatt actttgtatc
      421 tattctgagg gagatccttt ctttggtgta aatattaata gtcagaaaga attttggaac
      481 cactttgttt ctcagacaaa tcaaggtgga ccttatctgc aaaatcataa aataattgaa
      541 ctggtgtcca aaacttatcc tgagttggag ccatcgaaat taggaactat gctttttgag
      601 tattctaagc tttttatgga aaataaggaa gacaatagta caatggattc atcaaacaat
      661 tttcgtcatc aattaactca atctctatta aagtctccaa accttatcct tcgtggtgct
      721 cctggcacag gaaagactta tcttgctaaa gaaattgcca aagaattaac ggatggcaac
      781 gaagaccaaa tcggatttgt acaatttcac ccatcatatg attatacgga ttttgtagaa
      841 ggtttaagac cagtatcaaa tggggatgga gctattgagt ttaggctaca ggacggtatt
      901 tttaaagatt tttgtcagaa agcaaaagaa acccaattga ttggaggaca agataatttt
      961 gatgaggctt gggattctta cttagaatat ataaatgttg ctgaagaaaa agaatatata
     1021 acaaaaacat cttacttatc tattaatagt agacaaaatt tgtcagtaaa ttatgatagt
     1081 ggtgtttcag gatggtcact acctagcaaa tatgtttacg agttgtataa agataaaaat
     1141 tataataagc aagaatacta caaaagtggt ggaaaaactg tcctagaaac attgagaaag
     1201 agatttggtt tgaaagacta tgtttcccca acagaaattg atactgataa gaaattcgtc
     1261 ttcatcatcg atgaaatcaa tcgtggggag atttctaaga tttttggcga actctttttc
     1321 tctatcgacc ctggctatcg tggtgaaaaa ggaagtgttt ctacccaata tgcaaatcta
     1381 cacgaaactg atgaaaagtt ctatatcccc gaaaatgttt acatcatcgg aactatgaat
     1441 gatattgatc gttcagtgga tacctttgat tttgctatgc gtcgtcgttt tcgttttgtt
     1501 gaagttactg ccgagggtca agttggcatg ttggataaag agctgaatat ccatgcagaa
     1561 gaagcaaaaa ttcgtctaag aaacttgaac gctgctatcg aaaatgttca ggaattaaac
     1621 agtcattatc atattggacc aagttatttt cttaagttga aggatgtaga ttttgactat
     1681 gaattactct ggtctgatta tattaagcct ctcctagaag actacttgcg aggttcttat
     1741 gatgaggttg aaactttgga aactttgaaa aaagcatttg aactgacaaa taatgatcaa
     1801 actggtcaac aagatactgg tgataatgat gcggattact gataatcaat acaagattat
     1861 taaagaaaaa tttgttgaag aatatcctaa actaagcaat cttcttttag acagaacctt
     1921 ggaaagtcta tcccaagatg aacgtatttt catttttcca aatgatttga ctcatactcc
     1981 tgatttggat aaggaccaaa agatttttga aacagtcaat cagaaaatca agacagggaa
     2041 cgtgattggt tttcttggat atggtcagga aagattaacg atttcctcac gattttctga
     2101 tgagagtaat gaccattttt tgcattatct cttaaacaag gttcttcata tcaatctcac
     2161 tagtttagat gttgctttgt ctcgtgaaga gaggctttat caacttttga tgtatctctt
     2221 ccccaagtat ctacaagctg ctattcgaaa aggtctttat aaggaatacc agagattttc
     2281 tcataacgac agtcatgtta agggagtgat tgatgtaaga aaccatctca agaaaaatct
     2341 tcctttcacg ggaaatattg cctacacaac gagagagttc acctatgata atcccctcat
     2401 gcagttggtc cgtcacacta ttgaatacat taagaatcag aaaagcattg gtcaaggggt
     2461 actagataat ctctcaacta gtcgtgaaaa cgtatctgaa atcgtgcgtg taacgccctc
     2521 ttataaacta gctgatcgtg ctaagattat tcggggaaat caatctaaac ctatacgtca
     2581 tgcatacttt cacgagtaca gaaacttaca agaactttgt ctgatgatcc taaaccaaga
     2641 aaagcacggt ttagggtatc aagatcaaaa aatctatggt attctctttg atgttgcctg
     2701 gctttgggaa gagtatgttt acaccttgtt gccaaaaggt tttgtacatc ccagaaataa
     2761 ggataagacg gatggaattt cagtattttc tgttgggaaa cgaaaagtat atccagattt
     2821 ttatgacaga gaacgaaaga ttgttctaga tgcaaaatat aaaaaactgg aattgactga
     2881 aaaaggaatc aaccgtgagg acttattcca gctgatttcc tattcttata ttttaaaagc
     2941 tgagaaggct ggacttgttt ttccgagcaa ggacaaggtt attgataatg agatgggaaa
     3001 cctagcaggt tatggagctt tgttgaagaa atggtctatc caaattcctc agaagtcttc
     3061 atcttatagt gcattttgta aaatgatgga aaattcagaa gaaaatttta aagcgattat
     3121 tgatgaagaa gtggggagaa aataatttcc tctctttctt ttatttttaa cacaagtaaa
     3181 acccgaacaa tatatttcaa ttcataaaat aatttgacaa aaactgtact ttggtttata
     3241 ataaattaag gaaacgtcgg aaaagacgta gtgatgcgag agcataggta ggtcattaca
     3301 aaagaaacga gacatcgata tgttaaatga atttccaatt tttgattacg aagatattca
     3361 attgattcca aataaatgtg tcattaaaag ccgtgcagaa gcggatacaa gtgtcacttt
     3421 aggaaatcac acctttaaac tacctgttgt gccagcgaat atgcagacga ttttggatga
     3481 aaatgtagca gagcaactgg ctaaaggtgg ttacttctac attatgcacc gttttgatga
     3541 ggcaggacgc attcctttta ttaaacgaat gcacgatcaa gggctcattg cttctatctc
     3601 tgtcggtgtt aaggactatg agtatgattt cgttagacag ctcaagactg atgctcctga
     3661 gtatatcact attgatattg ctcatggtca tgcggatagc gtgatttcta tgattcaaca
     3721 catcaagaaa gaattgccag atacctttgt cattgctgga aatgtgggaa caccagaagc
     3781 tgtgcgtgaa ttggaaaatg ctggtgcgga tgctactaag gtcggaatcg gtcctggtaa
     3841 ggtttgtatc accaaggtta agactggttt tggtacaggt ggttggcagt tggctgctct
     3901 acgctggtgt gccaaggctg cgcgtaaacc gattatcgct gatggaggaa ttcgtactca
     3961 cggtgatatt gccaagtcta tccgcttcgg tgctagcatg atcatgattg gttccctctt
     4021 tgcaggacac attgaaagtc cagggaaaac gattgaagtc gatggtgaac agttcaaaga
     4081 atattatggt tcagcctcac aatatcagaa aggtgcttac aaaaatgtgg aaggcaaacg
     4141 tatcttgctt cctgctaaag gtcatctgca agacacttta actgagatgg aacaagacct
     4201 tcaaagtgct atttcttatg caggtggacg tcaggttgct gaccttaaac acgttgatta
     4261 tgtgatcgtg aaaaactcaa tctggaatgg ggatgcttcc cactaagacg gggtctttga
     4321 ctaaaaaact tgttattaga gcaaatttct gttataataa aacaagtttc cacccttagt
     4381 gtaatggata tcacgtaaga ttccggttct tgagatgggg gttcgattcc ctcagggtgg
     4441 atgtaaacat cctaaaaagc ctttaatagg gcttttttct ttacactgct ctaaatttgc
     4501 cgctaaaact gagaatctct gtctaaagaa ttcccaaatg ctttccagtc ccgttttaga
     4561 gtgactcagg gggctttttt tgatataata aaaaggactg ttatcagtta gaaagaggtt
     4621 ggtatgaaag aattacaaac tgtactaaag aaccattttg caatcgaatt tacagacaaa
     4681 aatttactgg aaactgcctt tactcatacg agttatgcca atgagcaccg cctcttaaaa
     4741 atttcacaca atgaacgctt ggaattttta ggagacgctg ttctacagtt attgatttca
     4801 gaatatctgt ataaaaaata tcctaaaaag cctgaaggtg acctatcaaa actccgtgct
     4861 atgattgtcc gtgaggagag tttagctggt tttgcgcgtg attgccagtt tgaccagttt
     4921 atcaagttgg gtaaagggga agaaaaatca ggtggtcgca atcgtgacac cattcttggt
     4981 gatgcctttg aagcctttct tggtgccctt cttttggata aggatgtggc caaggtcaag
     5041 gaatttatct atcaagtcat gattcctaag gttgaagcag gcgagtttga gatgattaca
     5101 gactataaaa cctatctcca agagttgctt caggtcaatg gtgatgtggc tattcgttat
     5161 caggtgattt ctgaaacagg gcctgctcac gataaggtct ttgatgtaga agttcttgtt
     5221 gaaggtaaga gcatcggtca aggccaaggt cgttctaaga aattagcaga gcaggaagct
     5281 gccaaaaatg ccgttgagaa agggctggat tcatgtattt aaaggaaatc gaaattcagg
     5341 ggttcaagtc ttttgctgat aagaccaagg tcgtttttga ccaaggtgtg acggcagttg
     5401 ttggacccaa tggatctgga aagtccaata ttacagaaag tctgcgttgg gctttggggg
     5461 agtctagtgt caagagtctc cgtgggggca agatgccgga tgtcatcttt gctggaaccg
     5521 aaagtcgcaa accgctcaat tatgcttctg tagttgtgac tctggataat catgacggat
     5581 ttatcaagga tgcaggtcaa gaaatcaggg tggaacgcca tatctatcgt agtggagata
     5641 gcgaatacaa gattgacggc aagaaagtcc gtctgcgtga tattcatgac ctcttcttgg
     5701 atactggatt gggacgagat tccttctcta ttatttccca agggaaggtt gaggagattt
     5761 ttaattccaa gtctgaggaa cgacgagcta tttttgaaga agctgctgga gttttaaaat
     5821 acaagactcg cagaaaagaa accgagagta aactgcagca aactcaggat aatctggacc
     5881 gcttagagga cattatctac gagttggata atcaaatcaa gcctcttgag aagcaagctg
     5941 agaatgcccg taagttttta gacttggaag gacaacgtaa ggctatttat ttagacgttc
     6001 tggttgctca aatcaaggaa aataaggcag aactagagtt gacagaagaa gagctggctc
     6061 aggttcaaga actcttgatg agttattacc aaaagcgtga aaaattagaa gaagaaaatc
     6121 aaactcttaa aaagcaacgc caagatttac aggctgaaat ggccaaagac caaggcagtt
     6181 tgatggactt gactagtctg attagtgatt tagaaagaaa attagcccta tcgaaactgg
     6241 agtccgagca agtggccttg aatcaacagg aggcacaagc tcgtttggct gctttggagg
     6301 ataagagaaa ttcactcagc aaagaaaagt atgataaaga aagctcttta gctctgttag
     6361 agggaaatct agtccaaaat aatcaaaaac tcaatcgttt agaagctgaa ttgctggctt
     6421 tctcagacga tcctgatcag atgattgagc tcttacgtga acgctttgta gctcttttac
     6481 aagaagaagc ggatgtctca aaccagttga cccgtattga gaatgagttg gaaaatagtc
     6541 gtcagctttc tcaaaaacaa gcagatcaac tagaaaagct gaaagagcaa ttggctatag
     6601 ctaaagagaa ggctagtcag caaaaggaag agcttgaaac tgccaaggag caggttcaga
     6661 aattattggc tgactaccaa gctattgcca aggagcaaga ggagcagaaa acttcctatc
     6721 aagctcaaca aagccaactc tttgaccgtc tggataatct caaaaacaag caggccagag
     6781 ctcaaagttt ggaaaatatc ctgagaaatc atagtaactt ttatgcaggt gttaagagtg
     6841 ttctccaaga aaaagatcgc ctaggtggga ttattggtgc agtcagtgag catctgacct
     6901 ttgatgttta ttatcaaact gccctagaga ttgccttagg ggcaagtagc cagcatatca
     6961 tcgtagaaga tgaagagtcg gcaaccaagg ctattgactt ccttaaacgg aacagagccg
     7021 gtcgtgcaac ctttcttcct ttgaccacta tcaaggcgcg tacgatttct agtcagaacc
     7081 aagatgctat cgctgtaagc ccaggtttcc ttgggatggc agatgagttg gtgacttttg
     7141 atactagact ggaagccatt ttcaagaact tgctagctac gacggctatc tttgataccg
     7201 tagaacatgc gcgtgcagca gctcgccaag ttcgttatca ggttcgtatg gtgacattgg
     7261 atgggacaga gttgcgcaca ggtggttctt atgcaggtgg tgccaatcgc cagaataaca
     7321 gcatttttat caagccagaa ctggagcaat tacaaaaaga aattgctgaa gaagaagcaa
     7381 gcttgggttc agaagaggcg actttgaaga ccttgcaaga tgagatggcc agattgaccg
     7441 agtcattaga agctattaaa tctcaaggag agcaggcacg tattcaggag caaggcttgt
     7501 tcctcgctta tcagcaaact agtcagcaag ttgaagaact ggaaactctt tggaaactcc
     7561 aagaagagga aatagatcgt ctttccgagg gagattggca agcggataag gaaaactgcc
     7621 aagagcgtct tgctgcaatc tccagtgaca agcaaaatct ggaagctgag attgaagaga
     7681 ttaagtctaa caaaaatgcc atccaagaac gctatcaaaa cttgcaggaa gagctagcgc
     7741 aagctcgttt gcttaagaca gaactgcaag ggcaaaaacg ttatgaagtt gctgatattg
     7801 aacgcttagg caaggaattg gacaatcttg attttgaaca agaggaaatc cagcgccttc
     7861 ttcaagaaaa gattgataat cttgagaagg ttgatacaga attgctcagt caacaggcgg
     7921 aagaatccaa aactcagaaa acgaacctcc aacaaggttt gattcgcaaa cagtttgagt
     7981 tggatgatat agaaggtcag ctggatgata ttgctagtca tttggatcag gctcgccagc
     8041 agaatgagga gtggattcgc aagcaaacac gtgctgaagc taagaaagaa aaggtcagcg
     8101 agcgcttgcg ccatctacaa agtcaattaa cagaccagta ccagattagc tatactgaag
     8161 ctatagaaaa agcgcatgaa ttggaaaacc tcaatctggc agagcaagaa gttaaggatt
     8221 tagagaaggc tattcgctca ctgggtcctg tcaacttgga agctattgac cagtacgaag
     8281 aagttcacaa tcgtctggat ttcttggata gccaacgtga tgacattttg tcagcgaaaa
     8341 acttgctcct tgagaccatt acagagatga atgacgaggt caaggaacgc tttaaatcaa
     8401 cctttgaagc tattcgtgag tcctttaaag tgaccttcaa gcagatgttt ggcggaggtc
     8461 aggcagactt gatattgact gagggcgacc ttttaacagc tggtgtggag atttctgttc
     8521 aacctccagg taagaaaatc cagtcgctta acctcatgag tggtggtgaa aaagctctat
     8581 cggctcttgc cttgctcttc tccattattc gtgtcaagac cattccattt gtgattttgg
     8641 atgaggtaga ggctgcgcta gacgaagcca atgtcaaacg ctttggagat tatctcaacc
     8701 gctttgacaa ggacagccag tttatcgtcg taacccaccg taagggaacc atggcagcgg
     8761 ccgattccat ctatggagtg accatgcaag aatcgggtgt ttcaaagatt gtttcagtta
     8821 agttaaaaga tttagaaagt attgaaggat gacaattaaa ctagtagcaa cggatatgga
     8881 cggaaccttc ctagatgaga atgggcgctt tgatatggac cgcctcaagt ctctcttggt
     8941 ttcctacaag gaaaaaggga tttactttgc ggtggcttcg ggtcggggat ttctgtctct
     9001 ggaaaaatta tttgctggtg ttcgtgatga cattattttc atcgcggaaa atggcagttt
     9061 ggtagagtat caaggtcagg acttgtatga agcgactatg tctcgtgact tttatctggc
     9121 aacttttgaa aagctgaaaa cgtcacctta tatagatatc aataaactgc tcttgacggg
     9181 taagaagggt tcatatgttc tagatacggt tgatgagacc tatttgaaag tgagtcagca
     9241 ttataatgaa aatatccaaa aagtagcgag tttggaagat atcacagatg acattttcaa
     9301 atttacaacc aacttcacag aagaaacgct agaagctggt gaagcttggg tcaatgataa
     9361 tgtccctggt gtcaaggcta tgacaactgg ctttgaatct attgatattg ttctggacta
     9421 tgtcgataag ggtgtagcta ttgttgaatt agctaaaaaa cttggcatca caatggatca
     9481 ggtcatggct tttggagaca atctgaatga cttacatatg atgcaggttg tgggacatcc
     9541 tgtagctcct gaaaatgcac gaccagagat tttagaatta gcaaagactg tgattggtca
     9601 ccataaggac cagtcggtta tagcttatat ggagggctta taatggcaga tataaaatta
     9661 atcgcattgg acttggacgg taccttgctg actactgata aaaggctgac ggatcgcacc
     9721 aaggcaactt tgaaagctgc gcgtgatcgt ggtatcaagg tcgtattgac aactggtcgt
     9781 cccttaaaag ccatggactt ctttctccat gagttaggga ctgacggtca ggaagatgag
     9841 tataccatta cttttaatgg tggtttggtt cagaaaaata caggtgaaat ccttgataaa
     9901 acagtctttt catatgctga tgtggcacgc ttgtatgaga aaacagagaa attatcactg
     9961 ccacttgatg ccatctcaga aggaacagtt tatcaaatcc aatcggacca agaaagtctt
    10021 tatgccaaat tcaatccagc tttgactttt gttccagttg actttgaaga cctgtctagt
    10081 caaatgacct acaacaaatg cgtgactgcc tttgctcaag aacccttgga tgcagccatt
    10141 cagaagattt ctccagaatt gtttgaccaa tatgaaatct ttaaatcacg tgaaatgttg
    10201 ctagagtggt caccaaagaa tgttcataaa gcaacaggtt tggcaaaact aatcagccat
    10261 cttggaatca accaaagcca agttatggcc tgtggtgacg aggccaatga cctttctatg
    10321 attgaatggg caggtcttgg tgttgctatg caaaacgctg ttcctgaagt aaaggcagcc
    10381 gcaaatgtag taatgccgat gaccaacgat gaggaagctg tcgcctgggc tatcgaagaa
    10441 tatgtgctaa aggagaacta agatatggga ttgtttgacc gtctattcgg aaaaaaagaa
    10501 gaacctaaaa tcgaagaagt tgtaaaagaa gctctggaaa atcttgattt gtctgaagat
    10561 gttgatccta ccttcacaga agttgaggaa gttcctcagg aagaagcaga ggttgaaatt
    10621 gttgaacaag ctgtgttcca agaggaggaa atccaagaca cagttgaaga aagtcaggat
    10681 tcagagccag ctgtaggggt ttctcaagaa gaaatagaag aattaccaaa ctcagaagaa
    10741 ttaccagagg aagagaagct tgagcacgaa ggaactgtag aagaaaataa ttctgaagtt
    10801 cttgaaccag aaaggcctca aacagaagaa actgttcagg aaaaatatga ccgcagtctc
    10861 aagaagactc gtacgggctt cggtgctcgc ttgaacgcct tctttgctaa cttccgctct
    10921 gttgacgaag aatttttcga ggaactggaa gaactgctga ttatgagtga tgttggtgtc
    10981 caagtcgctt ctaacttaac ggaggaacta cgttacgaag ccaagcttga aaatgccaag
    11041 aaacctgatg cacttcgtcg tgtcatcatt gagaaattgg ttgagcttta tgaaaaggat
    11101 ggtagctacg atgaaagcat ccacttccaa gataacttga cagttatgct ctttgttggt
    11161 gtgaatggtg ttgggaaaac aacttctatt ggaaaactag cccaccgcta caaacgaact
    11221 ggtaagaagg tcatgctggt tgcagcagat accttccgtg cgggtgcagt agctcagcta
    11281 gctgaatggg gccgacgagt agatgttcca gtagtaactg gacctgaaaa agctgatcca
    11341 gccagcgtgg tctttgatgg tatggaacgt gccgtggctg aaggtatcga tattctcatg
    11401 attgatactg ctggtcgtct gcaaaataag gataacctta tggctgagtt ggaaaagatt
    11461 ggtcgtatta tcaaacgtgt tgtgccagaa gcaccacatg aaaccttctt ggcacttgat
    11521 gcatcaacag gtcaaaatgc cctagtacag gccaaagaat tttcgaaaat cacaccttta
    11581 acgggaattg ttttgactaa gattgatgga actgctcgag gaggtgtggt tctagccatt
    11641 cgtgaagaac tcaatattcc tgtaaaattg attggttttg gtgaaaaaat cgatgatatt
    11701 ggagagttta actcagaaaa ctttatgaaa ggtctcttgg aaggcttaat ctaatcagaa
    11761 gcaaaaatcc tgcaaggtac aaacttgcag gattttttta ttcgaaacga ccatcttgac
    11821 gataggcgat atctggttgc caagtccatt tggcatcgaa tttttcaagt aggtcaaagc
    11881 tggcttgagg tcccatgctt ccagctttat agtcatgaag tggggcacca ttttcggccc
    11941 agagctcttc aatacggtca atcaacttcc atgacgcacc aacctcatcc cagtggctaa
    12001 agttagttga gttgttattt aggacatcat aaatcaattt ctcgtatggt tctggagaag
    12061 caccagttgc agtcgcgtct gtacgataat caagtgagtt aggagccaag ttaaattctt
    12121 ctcctacttg cttcccattt aggctaagag agaagccttc tgttggttga atatagatgg
    12181 tcaaaatatt tggagcaagt ggttctccaa agatagaatc catttgttta aagacgatgt
    12241 tgacatgagt tcctttttca gtcagtcgtt tacctgtacg gaaaaagaaa ggaacaccac
    12301 ggaatcgatc gctgtctaca aagaaggcac cagatgtaaa ggtttcagtt gttgattctg
    12361 gattcacatt tggttcgcta cgataagaga tgtatttcat gccatcaatc ttaccagagc
    12421 ggtattgtcc acggataaag tgttctttga gttcttcatc aattggatga tagaggtttt
    12481 taaagacctt aatcttttca gcacgaatct cgtcttttgt gaagcttgct ggtttgtcca
    12541 tggcgaggag cgaaagaagt tgtagagtgt ggttttggac catgtcacgg agggcaccgg
    12601 attggtcata gtagccacca cgttcttcta cacccaagcg ctccgcaaag gtaatttgaa
    12661 cattgtcgat aaaatccttg ttccaaacgt tttcaaaaat caagtttgca aagcgaactg
    12721 caaagatgct ttggatcatt tccttgccaa gataatggtc gatacggaaa atttgttctt
    12781 cgtcaaatgt tgctaggagt tcgtcattca acttgcttgc agttgcgtaa tctgtaccaa
    12841 atggtttttc aacgatcaag cgctcaaaac ctttgccatc gacaatgttt tcagatttga
    12901 ggtgtttggc aatggttcca aagaactgag gtgccataga caagaagaag agcttattgt
    12961 gttcagcttg gtatttttca ttaagctcag cttgtaattg acgcaaagca atataatgtt
    13021 ccgaatcatt gacatcatgg ctttgatagt agaagtggct agcaaattct tgggcttgct
    13081 cggtactatc tgccaaatca aggatggact cgacaactac agattcaaaa tattccttac
    13141 tccaaggtct acgggcagtt ccaataacgg caaagtgctt ggaaagattg ccggatttat
    13201 atagtctaaa aagggaaggg tagagcttgc gtttagccag gtctccactc gcaccgaaaa
    13261 ttgtaacaat aaccttagat gacatctagc tacctaattt ctatttttat tcctagtctt
    13321 gctaggtatg aggaaacctc atttcataaa cttaattcta acataatttt ccgaaaaatc
    13381 aaaaattcca atacgaggta gaaaaactgc aaggaaatcc ttacagtttg agtttagacg
    13441 tttaagacct tatctaagaa ctctttcaga cgagggtgtt gtgggttatc aaagatttgg
    13501 tcaggtgttc cgtcttcaag gaactcgcca tctgcagtaa agataacgcg gttggcaacc
    13561 tgacgagcaa atcccatctc atgggttacg ataatcatgg tcatgccttg ctcagccaat
    13621 tccttcataa cgttaagtac gtctccaacc atctcagggt caagggcaga agttggttca
    13681 tcgaagagca tgatgtctgg attcattgct aggccacgag cgatggccac acgttgtttt
    13741 tgaccacctg ataggctatc tggattggca ttagctttat ctgctagtcc aaccttttca
    13801 agcaactcca ttcccaattc ctcagcttct tccttagtca tcaacttgtg ctcaatagga
    13861 gcaaaggtga tgttgtccaa tacagacata tgagggaaga ggttgaagtg ttggaatacc
    13921 atgccgatat tttcacggac gtggtcaaca ttggttgttt tttcagttaa atcatagccg
    13981 ttcacagtga tgtgaccgct agtgacttct tctaaaagat tgaggctacg gaggaaagtt
    14041 gacttaccag aacctgaagg accgatgata caaacaacat ctccttcata gaacttagtc
    14101 gtaattcctt ttaggacttc attttttcca tagtgcttgt gtaaatcatt tacatcaatt
    14161 tttaattttg ccattaacga atcctctttt ctaagcgttt cgctagtcta gtcaaaagtg
    14221 tgataattac aagatagaag atagcaagga ttgcatacat cttgaaactt tggtagttac
    14281 gagcaatgat aatcttacca gtttggaaga gttcaaccaa accgatagca gatacgatag
    14341 ttgtatcttt aagagcgata acgaattggt tgacaaagtt tggcaacatc aatttagttg
    14401 cttgtggcaa gataatctta cgcatggttt ttccataaga gatacccaag cttcggctgg
    14461 cttccatttg gccaactgga acggcctgaa taccaccacg aacgatttca gcgatataag
    14521 cagccgcatt gagtgagagg gcaattgttc cagctacaaa gtcgttaatt gggctttgtt
    14581 ggcctgtgat agactcgatg aagtttggaa ttccccagaa gatgaaggct gcaagaatca
    14641 tcaatggaat accacgaata acgtcaacga aaatctcaga gataacgcga agagatttgt
    14701 atgggctaac gctaaacata ccgaagataa ttccgatgac aatggcaata gcaaatgaga
    14761 taagagctag agcaagagtg ataccaagac cgctaaggag ttgtttgtag ttgttttgaa
    14821 gcaagcccca gagcgttgtt tcgtcaacag tacttgttga agcagttgaa gattcgctag
    14881 ctaggtattt gtcaagaatc ttttggaatt caccgtttgc tttaaggttt gcaagtccgt
    14941 tgttgaacat ttcaatcagt tctggatttg ctcctttttt aacggcaaag gctgtttcac
    15001 cgattggagt tccagagatt ggagttttca atttttgacc ttggctgata gaatatttga
    15061 gaacaggttc atcatccata acggcatcaa tggcaccagt gtttaaactg tcatacattg
    15121 aagaaccatc agcaaaggtt ttgattttgt agccgtattt gctttgattt tctgttagga
    15181 aggtttgaga agcagttccg tttttaacac cgactgtctt tccttttaga tcttcataag
    15241 aagcaatatt gcttgattct ttgacaccaa gaatggtatt agcagtgtag tatgattctg
    15301 agaagtcaaa agttgcctta cgagcatctg tgacagacat accagcgatg ataccatcgg
    15361 cttgaccagc ttggacagca ctgatagcag catcaaaacc agggttggtg atttcaattt
    15421 caaaaccttg gtctttagcg attgccttaa tcaattccat atcaatacca gtgtattggt
    15481 tgcttgaatt ttggaaaaca aaaggtgcaa aagaagaatc gctggcaatg atatatttag
    15541 ccttaacagg aatagctttt aatcctgcga gagtagttgt agttggcact gctgaagatg
    15601 atgaagcagt ccatttcttg ataattttat caagactacc atcttttttc atttcagaca
    15661 aggcttggtt aaattcagta accaggtgct cgtatttact tccttttttc acaccgaaag
    15721 caaaacttcc tacagcttca ccatccattt caatatggag gtcttgacct tggttaatgg
    15781 catattcgat aacaggtttg tcatccatca tggcatcgat ggcaccagca ctcaagctgt
    15841 tgttcattaa atcaccagtg tcaaatgttt taatagtaaa gccgtattta tctttgattg
    15901 tttcaaggaa acgttgagcg gcagttccgt ttttaacacc aacggttttg ccagttaatt
    15961 ggtcgtactt gctaattttg tgtgactttg tagtagcaat gacaactttt gtatcatagt
    16021 aagtatcaga catggtgaag actttttcac gttctttagt ctttgtcatc cctgccatga
    16081 tagcgtcggc ttgcccagct tgaaccgcat tgactgctgc gtcaaatcca ggataggaca
    16141 tctgaatgtt ccagccttta atctcagcga ctttgttaat aatgtcaaca tcaattcctt
    16201 tataagtttg atctgaatct ttaaactcaa aaggtgcata ggcggtatca gaaacaatct
    16261 taatcgtttc tgctttggca atacctaatg agaaaattgg gaataaaatt agcaaaaatg
    16321 ctagaaattt tttcttcatt tttagtctcc ttttccgaat attctctcat tatatcagaa
    16381 aaagtaaaaa aaggcaaatt tttatatttt ttgtgtcaga aaatataaca tcgatttttt
    16441 cctttccttg aattgctatt aaaaagtgct ataataatag tatatataga agaaaagagg
    16501 acggggatat gaagaacaaa agaatattta aagacttcca agcttcaaaa atgagtttaa
    16561 acatttacac aagccccttg ttagcctttg tttttgtctt cataggagag tttgtggctt
    16621 ttactttgta tggtattggc ttgttagctc tcatcggact tgctagaaat tttggagagg
    16681 ctggtcaaaa tcttgcaagc tacttgcaga ccttgcatca gagcttgacg gataaaacaa
    16741 gtgactttcg tttaatttta ggattactgg cctttggttt tattcttaac actgtgttca
    16801 gatggacaag aaaagttgag aaaagaccta ttcgaacctt gggattttat agagagaatt
    16861 tcctcagcaa tcttctgaaa ggatttagtc taggcctggc actttttctt ctgaccttgt
    16921 taggtttagt ggtcttaggt caatatcgtt tggaatccat tcacttgaat ccttattctc
    16981 ttgcctttgt cgtctttact atcccatttt ggattttaca ggggacagca gaagaagtgg
    17041 tggcccgtgc ttggctactt cctcaattgg cctcaagaac caatctaaaa ctagctattc
    17101 ttatatctag cctgttcttt accctgcttc atatgggcaa ttctggtctc acccctctat
    17161 ctctagtaaa tctcttttta ttcggagttg ccatggctct ttaccttctc aaaactgata
    17221 cagtttgggg tgttgcaggt attcatggtg cttggaattt tgctcagggt aatctctttg
    17281 ggattttagt tagtggtcaa ccgtcaggaa cgtctctgat gaccttttta ccacaaggca
    17341 atcaagattg gctatcaggt ggttcttttg gcatagaagg ttccattatg acaagtctgg
    17401 tattactact gctgattgtc tatcttgcta ataaattaaa gaaagaaaat gaaaggatgt
    17461 gacttcggtc cgtccttttc ttcgtgaaaa tactataagt atgctaaaat aggaatagca
    17521 catggagaga ggattcttat gatcaatcac attacagata atcaatttaa actagtatca
    17581 aaatatcaac catcaggaga tcaaccccaa gctatcgagc agttggtgga taacattgag
    17641 gggggagaaa aagctcagat tctgatgggg gcgactggaa cagggaagac ctatactatg
    17701 agtcaggtca tttctaaagt caataaacca actctggtta ttgcccacaa taaaactctg
    17761 gctggtcagc tctatgggga gtttaaggaa tttttccctg aaaatgcagt tgagtatttc
    17821 gtatcctact atgattatta ccagccagag gcctatgtcc cttctagcga tacctatatt
    17881 gagaaggata gttctgtcaa tgacgagatt gacaagcttc gccactcagc tacctcagcc
    17941 cttttggagc gtaatgatgt tattgtcgtg gcctcagtct cttgtatcta tggtttgggt
    18001 tcgcccaagg aatacgctga tagtgtcgtt agtctccgtc ctggtctaga gatttctcgt
    18061 gataaactct tgaatgactt ggtcgatatt cagtttgaac gtaatgatat tgatttccaa
    18121 cgcggaagat ttcgcgttcg tggggatgtg gtagagattt tcccagcttc ccgagatgaa
    18181 catgcctttc gagtagaatt ttttggagac gaaattgacc gtattcgtga agttgaggct
    18241 ctgacaggtc aggtgttggg agaagtggat catttagcga ttttcccagc gacacacttt
    18301 gtgaccaatg acgaccacat ggaagttgcc attgcaaaga ttcaggccga gttggaagaa
    18361 caattagctg tctttgaaaa ggaaggtaaa ctgcttgaag cccagcgttt gaaacagcgg
    18421 acagagtatg atatcgaaat gttgcgtgag atgggctata ccaatggggt tgaaaattat
    18481 tctcgccaca tggatggacg gagcgaagga gagcctcctt atacgcttct cgacttcttc
    18541 ccagatgatt tcttgattat gattgacgag agtcatatga ccatagggca aatcaagggc
    18601 atgtacaatg gagaccgttc gcgtaaagaa atgctggtta attatggttt ccgtttgccg
    18661 tctgctttgg acaatcgtcc tctccgtcgg gaggagtttg agagtcacgt tcatcagatt
    18721 gtttacgttt cagcgacacc tggtgactat gaaaatgaac agaccgagac agtgattgag
    18781 caaatcattc gtccaacggg actcttggat ccagaggtgg aagtccgtcc gactatggga
    18841 cagattgatg acctcttggg tgaaatcaat gcccgcgttg aaaaaaatga gcgtaccttt
    18901 atcacaactt tgaccaagaa aatggcagag gatttgaccg actacttcaa ggaaatgggt
    18961 atcaaggtca agtacatgca ctcggatatc aagaccttgg aacggacgga gattatccgt
    19021 gacctgcgct tgggtgtctt tgatgtcttg gtcggaatta acctgctccg tgaaggaatt
    19081 gacgttcctg aagtgagcct cgtagctatt ctcgatgctg acaaggaagg tttccttcgc
    19141 aacgaacgtg gactcatcca gaccattgga cgtgctgcac gtaatagcga aggtcatgtt
    19201 atcatgtatg cggacacggt tacccagtct atgcaacgtg ctatcgatga aactgcccgc
    19261 cgtcgcaaaa tccagatggc ctataatgaa gaacatggta tcgttccaca aaccatcaag
    19321 aaagaaatcc gtgacttgat tgctgtgacc aaggcagttg ctaaggaaga agacaaggaa
    19381 gtcgatatca atagcctcaa caaacaagag cgcaaagaac tagtcaaaaa gcttgagaaa
    19441 caaatgcaag aagcagttga agtgcttgac tttgaactag cagctcagat tcgtgatatg
    19501 atgctggaag tcaaggcctt ggattagggg aatagtatga tttatttaag aaagttaaag
    19561 aaagaagatt tgatgtcttt atgggaaatg gcttattcac agcttaatcc agtttggaaa
    19621 cagtatgatg ctccctatta tgatgattat cagtattttt caaattttaa agaattcgaa
    19681 ctacaaaaat cagaatccat tttaagcaac tcaaatcgcc ttggtatttt tgttgatgat
    19741 aaactagttg ggactgtttc gcgttattgg gtatgtaaag aaacaagatg gatggaattg
    19801 ggaattggta tttatgataa aaaattctgg aacactggta ttgggaaagt tgctatgttg
    19861 cagtggatag ataggacgtt tcaggattac ttggagttgg agcatctggg tttgacaact
    19921 tggtcaggaa atattggtat gatgaaactt gctgaaaaat taagaatgaa aaaagaagct
    19981 catattccaa aagttcgtta ttatcaaggt aaatattttg acagtattaa atatggtatt
    20041 ttgagagaag actgggagaa aataaatgac ggttattatc aaatcaatgg aaactcctga
    20101 agagatagaa ggtaaatcct tcgttcactg gcaaacgtgg agagaggctt atgatgacct
    20161 tttgcctgcg gaatttcagg agacaatgac attagaaaga tgtcgactct ttagtcaaaa
    20221 gtatccagaa aatacattga ttgcgatgga tggtgtgaag atagttggtt ttataagtta
    20281 tggcaactgt cgtgatgaga ctattcaagc tggtgaaatt attgctttat atgttttaaa
    20341 agactattat ggaaaaggaa tcgcacaaaa gttagtgaaa gcagctttga ctgatcttaa
    20401 tcatttttct gaaattttct tatgggtatt gaaagataac aagcgcgcca ttgctttcta
    20461 tcaaaaaatg ggttttactt ttgatggaca agaaaaaata cttgaacttg gaaagcctat
    20521 aaaggaaaaa cggatggtat tctattctaa ataattctca aaagtaaaag ctaatatggt
    20581 accaagtctg aaaatttaat aaattagaaa gcgagtaaat ttatgtcccg ttcccaatta
    20641 acgattttaa caaacatttg tctgattgaa gacctcgaaa ctcagcgcgt ggtgatgcag
    20701 tatcgctctc ctgaaaccaa tcgctggtct ggttatgcct ttccaggagg ccatgtagaa
    20761 aatggcgagt ctttcgcgga gtctgtcatt cgtgaaatct acgaagaaac aggattgact
    20821 atccaaaatc ctcaactggt cggtatcaag aactggccac tagatacagg cgggcgctac
    20881 attgtcgttt gttataaggc aactgagttc tctggtaccc ttcaatcttc agaagaggga
    20941 gaagtttctt gggtgcaaaa agaccagatt ccaaacttgg atctggccta tgatatgtta
    21001 cctttgatgg agatgatgga cgctcctgac aagtcagagt ttttctaccg ccaccgtaca
    21061 gaagacgatt gggaaaagaa aattttctaa tcctttacta aataaccaag ctgatccaag
    21121 gcctcctcga tatagtggag gtcttgttgt gtctcggctt caactaggtg ataatgaata
    21181 ccatctgtta actcagaaat tggcttaaag tcagaacgtt caacttgttc tagaaaatgt
    21241 tgcacgtcgc ggcgacaggt cagttttagt aaggtttcaa tctctccata aacaggatga
    21301 tcaatcaaga tattttgaac gcgaccacca ttatctacga tagcaagtaa ttctcgtcca
    21361 atttcttcaa cttcatgctt gaccttaaat aatttgtgat gataagtatt tgcattagca
    21421 tctttataga tataaccacg attggtagat agaattggag atccatcagc tcttaaaatt
    21481 gcaatatctt gaacaataac ttgtcgagtg acatgaaagt gctcagctaa tctttggcca
    21541 ttgagggctt taggagcttc tttcaagagt tggagcaggg cttgtttgcg atcttttgtc
    21601 atagttcttc cttttaacgg cgttttcgaa gcactttata gacagctagt gctaatgtat
    21661 agtctaccat actatggata attgtaccaa atccaactag tacaaataga acataaaaca
    21721 tattttctac attggtacca gaagttgcgt aaaaaacgac acaggccaat acttcagcaa
    21781 gggcatgaac aacagccaaa acaaagttga aaatccagga agattttggt ttatctaggg
    21841 tatcggggaa tttttgtagg taaagagctc ctaaagcacc aaaagatata tgggaaaaag
    21901 cccgaaaaac gataaccatg ggatagccag ccatcaaaaa tccaaaacta gaggctagga
    21961 tgacaaaaac tgccatcaag ggcgacaaga acatggctat aaaaatagcg atgtggctcc
    22021 ccaaagtata ggaagcaggt ggaatgacaa tcttgaaagg cataacaatt ggaatcaaaa
    22081 tcgcaatagc cgttaaaagg gctgtcattg tcataaattg tgtctttttc cgtgtattca
    22141 caagaatctc ctttttaact gcatatacac tagtatggta caataaacca gacaataaag
    22201 caagaattta cttgggttta tagatcattt tttagttaaa agttatagta gattgaaact
    22261 agaatagtcc acctctactt ctaaaacatt gttagaaatc gatttggctg tcctgatcga
    22321 tttgtcctgt tcttatttcg ttttactata gtaaagattt cattaaaaag aaactgtata
    22381 gagcaaaatc tccaccttca ggtttgaaag cggagattgt ttttattttt tcaaggtttg
    22441 tagtcgtgga acaatggcta gggttagaat tgcagaaatg accaattcag caattgaatt
    22501 tgttgagata acggttgcca gaagaagttg gatatttcca ttataaacat ttccaaatag
    22561 gaagaagatt cctccaagga caaagatagt atttgtcaag gaaccaaggg ctccagctaa
    22621 aatcagacca gtcttgtttt tcatcagttt atagactaag taaggagtta aaccaatcaa
    22681 aatacgtggg acgatggcaa tgatagctga gtagatgttt ccgtttggta cgaagggaga
    22741 gaagaggtag cttgtcggta gaatcgtaat cgtgttaacc gtcaagctaa gtaatcccat
    22801 caaaaatcca agtgtaaccc caactcgtgg accataaata atgctggcaa taatgacagg
    22861 aatatgaaca atggtcggtt tgattggaaa tggaaaaagg ttaaagataa gtgagctcag
    22921 aaagtgtatc acgagcatgg tagcaaaaaa gatagcaatg ggtgcaatat tagagcgttt
    22981 tttcatcgat agtttccttt attctttcta aaataattgt gaggtcagct aaagctcctc
    23041 gtccgtggtc tccacaagct agtagggatt ccttaggagc aatcagctga tagccgtagg
    23101 tttctaatgt tttcagatta ttctgagtta ctggatggtc atacattttt gtattcatag
    23161 caggagctat tagtttggga atatgacttg gtagggctag agctgtactg gttaccatgt
    23221 tgtccgcaaa tccgtgagct agttttgcaa tagtgttagc agttgcaggt accacgataa
    23281 ataaatctgc tttttttcca agttcgatat gattgacctg atcaggatag ggttccttca
    23341 tgacatccaa gtggacagga ttctgtgaga gtacctgtag tgtcaaaggt tggataaact
    23401 ctgtagcagc ctgagtcatt aagacagtga cttgatggcc ttgttttttt agagaactga
    23461 ctaaatctgc cgacttataa gaggcgattg aacccgttac agccaagaga atgtttgcca
    23521 tagttttcct ttctaagaat gataggcttg aattttttca aggaggagtt ctgcaatttc
    23581 ttctttagtc tggactgttt gaagctgatt tttctcaaca aatatagctc ggtgctgatc
    23641 tgctgaaatt tgagtcaggt cattcgcgat gattaaatct gcttgattct tgataagact
    23701 ttttcgtgca atgtcaacca gatgatcttc ggtaacatca accagcagtt tgaaaccaat
    23761 cagatgaata gtaggattcc attccttgac tagggatatg attttgggtg tctttttaag
    23821 gaacaaaacc tgaacctcat cagttgaaga aatcttggcc tgatgatttt gcttgcttaa
    23881 aaattctttt agattggagc tagcctgaac ttcctcaagc cctgtcatat aaacaggagt
    23941 gtagtcagaa acagccattg agtggatcaa gacctgataa tcctgaacac gttcttgcat
    24001 ttctattaga aggtccttgg tattggtaat ttctcgaata cttaggttag gatgaggctc
    24061 tggcttcaga gctcgttttg tcgtaattaa acaaacttca taccctgcag aaagcaaagt
    24121 ctctgtgata attttcccca agtgacctgt agaatggtta gtgatagagc ggacgctatc
    24181 gatagcttca ctggtaccgc ccgatgtaac taaaattttc atagcactat tgtacacaaa
    24241 aataagcggt aagtaaaatg aaagtgataa atttttggta aaaacgaaga tttattatag
    24301 tttcaaacta taaagccata taaaatttaa gaaaggactc taaaagtctt aaaaacagag
    24361 atatttacga acgtttagag agttttagga tgttaaaaat cttgttttgt gatataatga
    24421 attatcatat aagaggttag aggaattttg aatgaaaaca gatattgaaa tcgcacagag
    24481 tattgagttg aagccaattg ttgatgttgt agagaaactt ggtatttctt atgacgattt
    24541 ggagttgtac ggaaagtaca aggctaaact cagctttgat aaaattcgtg cagttgagag
    24601 caatccagtc ggtaaattga tcttggttac tgccatcaac ccaacacctg caggtgaagg
    24661 aaagtcgacg cttaccattg gtcttgcgga tgccttgaac aagattggca agaaaaccat
    24721 gattgctatc cgcgaaccgt ctcttggtcc agtcatgggg atcaagggtg gtgctgctgg
    24781 tggtgggtat gcacaagttc tgccaatgga agacatcaac ctccacttta ctggagatat
    24841 gcatgctatt acaactgcca acaatgccct ttctgccttg attgacaacc acttgcacca
    24901 agggaatgag ctgggaattg atcaacgtcg tatcctctgg aaacgcgttg tggacttgaa
    24961 cgaccgtacg ctccgccatg tgactgttgg tcttggtggt cctctaaacg gtattccacg
    25021 tgaggatggt tttgatatta cagttgcttc agaaatcatg gcaattcttt gcttggcaac
    25081 ggacatcgag gacttgaaac gtcgtttggc gaatatcgtt attggttatc gctatgaccg
    25141 tacgcctgtt tctgtaggtg atttgcaggt tgagggtgcc ttggctttga ttttgaagga
    25201 tgctattaag ccaaacttgg ttcagacaat ttacggtaca cctgcctttg tacacggtgg
    25261 tccatttgcc aatatcgctc atggctgtaa ctctgttttg gcgacgacaa cagcccttca
    25321 cttggctgat tacactgtta ctgaagctgg ttttggtgcg gaccttggtg ctgagaaatt
    25381 ccttgatatc aagacaccaa acttgccaac atctccagat gcagttgtta ttgtcgcaac
    25441 ccttcgtgcc cttaagatga atggtggtgt ggctaaagac gctctgactg aagaaaatgt
    25501 agaggcagtt cgtgcaggtt ttgctaactt gaaacgccac gttgaaaata tccgtaagtt
    25561 cggtattcca gcagttgtag ctattaacga atttgtatct gatacagaag ctgaaattgc
    25621 agccttaaaa gaactctgtg cctcaatcga tgtaccagtt gaattggcta gtgtctgggc
    25681 tgatggagca gaaggtggag tagcacttgc cgaaacagtt gttaagacaa ttgctgaaaa
    25741 tccagctaac tataaacgtt tgtatgataa tgacctttct gtccaagaaa agattgaaaa
    25801 aattgtcact gaaatctatc gtggtagcaa agtgaacttt gagaagaaag ctcaaacaca
    25861 aattgctcaa atcgttcaaa acggttggga caaattgcca atctgtatgg ctaaaactca
    25921 atacagtttc tcagacaatc caaatgcgct tggagcacca gaaaactttg aaattaccat
    25981 tcgtgaattg gtaccaaaat taggtgcagg cttcatcgtt gccttaactg gtgatgttat
    26041 gaccatgcca ggtcttccaa aacgtccagc agctctcaac atggatgttg aaagcgatgg
    26101 aactgtacta ggcttgttct agtggattga aattgacttt aagtgagttt ggaaataata
    26161 tccaagctca ttttttctca gactgcaagc agttcatcaa taagatttat actcttcgaa
    26221 aatctcttca aactacgtca gcttcacctt gccgtaggta tatgatactg actctgtcag
    26281 ttctatccac aacctcaaaa cagtgttttg agcaacctgc ggctagcttc ctagtttgct
    26341 ctttgatttt cattgagtat aagtagatag aaaagttata tactatctat tgaaaatatt
    26401 ataaatcaga tacaaagaca ggtgaaacct tctgtgttat actagagata tgttagattt
    26461 gaaagaatac ggtatcgtca tgtggccgga ggagaaagtc atttctttcc gtgagaaact
    26521 tctcgcttgg tatgatgaaa acaaaagaga tttgccttgg aggagaagta aaaatcctta
    26581 tcacatctgg gtatctgaaa tcatgcttca gcagaccagg gtggatacag ttatccctta
    26641 ctacgaaaga tttttggact ggtttccaac tgtcgaaagt ctggcaactg cgcctgagga
    26701 gagtttactg aaagcttggg agggcttggg ctattattct cgagttcgca atatgcaggc
    26761 tgcagcccag cagattatga ctgactttgg tggccaattt ccaaatacct atgaaggaat
    26821 ttccagcttg aaagggattg gaccttacac agcaggagcc atttccagta ttgcttttaa
    26881 cttgcctgag ccagctgtag atggtaatgt catgcgggtc ttggcgcgtc tgtttgaagt
    26941 caaccacgat attgggattc caagtaatcg aaaaattttt caggcaatga tggagatctt
    27001 gattaatccg gatcgtccgg gtgattttaa tcaagccttg atggacttag ggtctgatat
    27061 tgagtctcct gtaaatccca gacctgaaga aagcccagtc aaggacttta gtgcggcata
    27121 tcagaatgga accatggacc gttatccaat taaatctcct aagaaaaagc cggttcctat
    27181 ttatcttaaa gccttagttg tgaaaaattc tcaaggacaa tttttacttg aaaaaaatga
    27241 aagcgaaaag ctcttggcag gtttttggca tttccccttt atagaagttg ataacttttc
    27301 gcaagaagag cagtttgacc tctttcatca ggttgcggaa gaaagtgtga actttggtcc
    27361 cagtccagaa gagagtttcc agcaggacta tgacctagat gttgattggc ttgatgtttg
    27421 ttttgataca gtccagcatg tttttagtca tcgaaaatgg catgtacaga ttgtagcagg
    27481 tcaggtgagt gacttccatg atttttcaga tagggaagtt cgctggcttt caccagaaga
    27541 attcaagaat tacccacttg ccaaacccca acaaaaaatc tggcaggttt atgcacaagc
    27601 caacttagac agtagccaag actagctatt gtgtcttctg actatttttt tggtataata
    27661 gtagagaaaa aggtgaacat atgaaaaaaa tactaattgt agatgatgag aaaccaatct
    27721 cggatattat caagtttaat atgaccaagg aaggttacga agttgtaact gcttttaatg
    27781 gtcgtgaagc gctagagcaa tttgaagcag agcaaccaga tattattatt ctggatttga
    27841 tgcttccaga aattgatggt ttagaagttg ctaagaccat tcgtaagaca agcagtgtgc
    27901 ccattcttat gctttcagcc aaagatagtg aatttgataa ggttatcggt ttggaacttg
    27961 gggcagatga ctatgtaaca aaacccttct ccaatcgtga gttgcaggcg cgtgttaaag
    28021 ctcttctgcg tcgttctcaa cctatgccag tagatggtca ggaagcagat agtaaacctc
    28081 aacctatcca aattggggat ttagaaattg ttccagacgc ctacgtggct aaaaaatatg
    28141 gcgaagaact agacttaacc catcgtgaat ttgagctttt gtatcattta gcatcgcata
    28201 caggtcaagt catcacgcgc gaacacttgc ttgagactgt ctggggttat gactattttg
    28261 gtgatgtccg tacagttgat gtgactgtac gacgtctgcg tgagaagatt gaagatacgc
    28321 ccagccgacc agagtatatc ttgacgcgcc gtggtgtagg gtattacatg agaaataatg
    28381 cttgatttac tgaaacaaac catttttacc agagatttta tctttatcct gattttgtta
    28441 ggtttcatcc ttgttgtgac cctcttatta ctggaaaata gacgtgataa tattcagttg
    28501 aagcaagtca atcaaaaggt taaagatttg attgcaggag attattccaa ggttcttgat
    28561 atgcaaggtg ggtctgaaat caccaatatt accaataatt tgaatgactt gtcggaggtt
    28621 attcgtctca ctcaggaaaa tctagaacaa gagagtaaga ggctaaatag tattctgttt
    28681 tatatgacag atggggttct tgcgactaac cgtcggggtc agattatcat gattaacgat
    28741 acagccaaga agcaactggg gttggttaag gaagatgttc tgaatagaag cattttggaa
    28801 ttgctcaaga tagaagaaaa ctatgaattg cgtgatttga ttacccaaag tccagaattg
    28861 ttgctagatt cccaagatat caatggcgaa tatttgaacc ttcgagttcg ctttgccttg
    28921 atacgtcgag agtctggctt tatttcaggt ttggtggctg ttttgcatga tacgacggag
    28981 caggagaagg aagaacgcga acgaagactc tttgtttcca atgttagcca tgagttacgg
    29041 actcctctga ctagcgtaaa atcctatctt gaagccttgg atgagggggc tttgtgtgaa
    29101 actgtagcac cagactttat caaggtttct cttgatgaga ccaaccgtat gatgcgcatg
    29161 gtgacggatc tcctccatct ttcacgtatt gataatgcta ccagtcacct agatgtggaa
    29221 ctgattaact tcactgcttt tattaccttt atcctcaatc gttttgacaa gatgaaagga
    29281 caggaaaagg agaaaaaata tgagttggtg agagattatc ccatcaattc tatctggatg
    29341 gaaattgata cagataagat gacgcaggtt gtcgacaata ttttaaataa tgctattaag
    29401 tattcgccag atgggggtaa aatcactgtc agaatgaaga caactgaaga ccagatgatt
    29461 ttatccattt ctgaccacgg tttggggatt cctaagcagg atttaccacg tatctttgac
    29521 cgtttctatc gtgtggatcg tgctagaagt cgtgcacaag gtggtacagg tctaggactg
    29581 tctatcgcta aagaaattat caaacaacat aagggcttta tttgggccaa gagtgaatac
    29641 ggcaagggtt caacctttac cattgtactc ccttatgata aggatgcagt gaaagaagaa
    29701 gtatgggagg atgaagtaga agactagaat gagtgaaata ggctttaaat acagtatttt
    29761 agcgtcgggt tccagtggaa attcttttta tctggaaacc tcaaaaaaga agcttttagt
    29821 agatgcaggc ttgtctggca agaaaattac cagtctgcta gctgaaatta accgtaagcc
    29881 agaagacctg gatgccatct tgattaccca tgagcattca gatcatatcc atggagtagg
    29941 cgttttggct cgcaagtatg gtatggatct ttatgccaat gaaaagacct ggcaagctat
    30001 ggaaaatagt aaatatcttg gcaaggtgga ttcttcgcaa aagcatattt ttgaaatggg
    30061 taaaaccaaa acctttggag atatcgacat cgagagtttt ggtgtaagcc atgatgcagt
    30121 cgcaccgcag ttctatcgct ttatgaagga tgataagagt tttgtcctct tgacagatac
    30181 aggttatgtc agtgaccgta tggcgggcat tgtcgaaaat gcggatggtt atcttatcga
    30241 ggccaaccat gatgtagaga ttttgcgatc aggttcttac gcttggcgac tcaaacaacg
    30301 aatcctatct gaccttggtc acctttctaa cgaggacggt gctgaagcta tgattcggac
    30361 gctaggaaat cgtactaaga agatttacct tgggcattta tctaaggaaa acaatatcaa
    30421 ggaactggct catatgacca tggtcaatca gctggctcaa gctgatctgg gagtcggagt
    30481 agactttaag gtttatgata cctcaccaga taccgcaaca ccattgacag agatatagaa
    30541 agaacgctga gaaggtgttc tttttatatt gactgaacac ctaaaaagta atacaatggt
    30601 gttaccatta aaaaagggag cacaaaagat gactactatt acattaaaag tttctgaagc
    30661 tgataaaaca tttatgaaag caatggctaa gtttgaagga gtttcccttt cggaacttat
    30721 tcgcaccaaa actcttgaag ccctagaaga tgaatacgat gctcgtgtgg cagatttagc
    30781 ctatcaagag tatttagaag acttggaaaa aggagttgaa cccattactt gggaagaaat
    30841 gatgcatgat ttaggcttga aggatgaata atttgtataa attagttcca acaagacgtt
    30901 ttatcaagca attgaaaaaa ttggaccgtt atacgcagaa gctaattaca aactatttac
    30961 aaaccaatgt tttggaagac ccaagacgac acggaaaggc tttggttggt aatcgcgttg
    31021 gtcaatggcg ctatagaatt ggtaattatc gagttatcgt acaaattgta gatgatgaat
    31081 tagtcgttgc tactctagaa gttggtcatc ggagagatat ttattgaatt acttttttca
    31141 agtaaatttt cattctttaa aatatgatat aatagggctt acaatataag agaggttgct
    31201 gatgacaata gatattagtg aagaaagttt ggctaaagaa tcggctgact tattaaaaat
    31261 tcttttaaaa gatcgaacga caaagaaatc aattgtttgg gctacgcatt cttatgaatt
    31321 gttaggaaag ggatttgctc caagtgatcg catcaatcca agtaaggtta ctggaaactt
    31381 tgccaacttg attcagccca gatctgaaaa atccaaatat gaacaaaaag atcggacaaa
    31441 aataagggca gaagtgttta caccaacgtg gttggtcgca aagcaaaatg gatatgtaga
    31501 atcgaaatta ggctcgttaa gtcttgagga gtatgtggac ttacgatggt tagaggtaac
    31561 ttgtggtgaa gcaccttata tggtaacgag atatgatacc gtaactggtg aggaaattcc
    31621 attatctgag cgagttggtt ttgttgatag aaaactgcaa cggattagtc gtgaggtttc
    31681 agatgaagtc acattttata aacttgtaaa agatgcttat catgcttcgt atggttatga
    31741 gtatcaagga gattctctcc ttttggcgcg tgagaatctt ttggcaactt ttgaggacta
    31801 ttatctagct aagactggaa atcaaccaac attagagcaa aagaaagaaa ttgcgactat
    31861 tatctcttac aatgtctttc agatggatgg attgaaaaaa agtagtcctt attcagctaa
    31921 acaaaagcaa tctcaacaat tgagtttgtt tgcggatgag ctagaagtcc aagaagtaga
    31981 agaatctaaa acccatatca aagactggaa aaagaataga atgataggtt ttgagcgcct
    32041 atctagcgag gaaagtgaaa tgaaatttga tgtcgtgata ggaaatcctc cgtatcagga
    32101 aacaggagaa gccagagatg agccgatata tcactatttt attgatgaag cttataaaat
    32161 cgcagataaa tctatactaa tcaccccagc aaggttttta tttaaagctg gtcaaactcc
    32221 taaaaattgg atggaaaaga tgcttgaaga caagcattta aaagttcaat tctatgaact
    32281 taaaagcggt aaagtattta caggaacgga tattaagggt ggagtagcaa taacttatcg
    32341 tgatgttgat aaagatttgg gaccaatcgg agtatttact atttttgaaa ctttgaatgg
    32401 tattgtaaaa aaaatcgaaa aacatccaga atttgaaact ttttctgatt tagtttatcc
    32461 tcaaggtatt tatagatttt caaataaact tttttctgat tatccaaatg ctgaaaagct
    32521 gcaaggaaaa gggacaaaaa ataaaattgt ttcgaaatca ctaactcagt tagattttgt
    32581 tttcaaaaac aacgaagaaa aagaccatgt tgctctactt ggaagaattg gctccgaacg
    32641 tgtttatcga tatattaata aaaaatattt agatttaccg gaaacattcg aaaattataa
    32701 tgtttttgta ccagaagcta atggaagtgg tgccttaggt gaagtcttat caacacccct
    32761 aatcggggaa cccctaatcg ggcatacaga tactttttta tctattggta attttaaaac
    32821 aaaatttgaa gccgatgctt gtattaaatt tattaaaact aaattcgcta gagtattatt
    32881 aggtgttttg aaagttactc agcataattc acgcaaaact tggtattacg tccccctcca
    32941 agactttacg gtcaattcgg acattgattg gacacaatca gtgactgata ttgaccgcca
    33001 gcttgatcaa aaatatgacc tttcccctga agaaattgcc tttattgaga atcatgtaag
    33061 ggagatggat tagaaaagta tttttatttg acaaatagtg ctcaatgatc taaaatgact
    33121 atataggatt aggtcaggaa gcatacgatg ccctgaccct ttttgtactt atgagatgag
    33181 aaagtcattt gttagataaa ttgacttcgt tagcaaacgt tcaaaaaagg aaaacttatg
    33241 ccagtagaaa ttaaaaccac taaagaaatt catcctaaaa tctatgccta caccacaccg
    33301 acagtaacca gtaatgaagg ctggattaag attgggtata cagaacgtga tgtcacacaa
    33361 cgtatcaagg agcaaacgca tacagctcat atagctacag atgtcttatg gactggtgat
    33421 gcagcttata cagaagagcc tgataagggg aaaactttca aggaccatga tttccaccat
    33481 ttcctttctt tccatgatgt agaacgtcgt cccaagacgg aatggttcta ttttaatgga
    33541 actcctgaaa aatcaaaaaa tctttttgat aagtttgttc agcatgattt gtctggttat
    33601 cagcctggaa aaggacagga ctatactctg cgacaagagc aagaagaagc agttgctaag
    33661 acattagctt atttccaaga acatgctgga ggcaagtttc tctggaatgc caagccacgc
    33721 tttggtaaaa ccttgtctac ctatgaccta gctcgacgga tggaagctgt caatgtccta
    33781 attgtaacaa accgccctgc cattgctaac tcatggtatg atgattttga aacattcata
    33841 gcaggtcaaa cgacttacaa gtttgtttct gaatcagata gccttaagag tcgtccaatc
    33901 ttgtcacgac aagaatttct tggtatttta gctgacgatg taagacaact tgcttttatc
    33961 agtctccaag acttgaaagg atctgtttat ttaggtggag agcacgataa actcaaatgg
    34021 gtaactgatc tgcattggga cttgttggtt attgacgagg ctcatgaagg agttgatacc
    34081 ttcaagactg accaagcctt taataagatt cgacgaaatt ttactctgca tttgtcaggt
    34141 acatcattta aagcattggc taaaggagat tttacagagg aacaaatcta caactggtct
    34201 tatgctgatg agcaggctgc taagtattcg tggtctcttg agcaagaaga ggaaaatcct
    34261 tatgaaagct tgcctcagtt gaatctcttt acctatcaaa tgtctcagat gattggcgaa
    34321 aagttagaaa aaggcgctca gatcgatggt gaaaatattg actatgtttt tgacttaagt
    34381 gaatttttcg ctacagatga taaagggaaa tttattcatg agcatgatgt cagaaattgg
    34441 ttagatactc tatcaagcaa tgaaaaatat ccattttcaa ccaaagaact ccgtaatgaa
    34501 ctcaagcata ctttttggct tttagaacgt gtcgcttcgg ccaaagcatt aaaagcccta
    34561 ctagaagaac acccaatcta tgaaaactat gagatcgttc tagctgctgg tgacggacgt
    34621 atgtccgaag aagacgataa agtcaaactc aaatccttgg acttggttag aaaagcgata
    34681 gcagagaatg acaaaaccat taccctatcc gttggtcagc tgacgacagg tgtcactatc
    34741 cctgaatgga caggtgtatt gatgttatca aatttgaaat caccagctct ttatatgcag
    34801 gccgccttcc gtgctcaaaa tccttactca tggagcgata acaaaggaaa tcactttcgc
    34861 aaagaaagag cctatgtatt tgactttgcg ccggaaagaa ccttgattct ctttgatgag
    34921 tttgccaaca acttatcgct tgtaactgca gctggtagag gaacttcagc tacacgcgaa
    34981 gaaaatatta gagaattatt aaacttcttt ccaattattg ccgaagaccg tgctggtaag
    35041 atggttgaaa ttgatgcaaa ggcagttcta accactcctc gccagataaa agctagagaa
    35101 gttcttaaac gaggttttat gtccaatctc ttatttgata atattagtgg tattttccaa
    35161 gcaagtcaaa cagttttaga tattttaaat gagctgccag ttgaaaagga agggaaggta
    35221 caagatagtt ctgatttatt agatttttca gatgttacag tcgatgatga gggaaatgca
    35281 gtagtagacc atgaaattgt agttaatcag caaatgcgac tttttggtga aaaagtttat
    35341 ggacttggtg aatctgttgc tgagttagtc acaaaagatg aggaacgaac tcaaaaacag
    35401 ctggtcaatg acttgagtaa gaccgtttct tcagtgattg tagaggaatt gaaagcagat
    35461 tattctctaa aaacaaggga aactgagcaa attaagaaac aaattacagc aacacttgag
    35521 aatgaaattc gaaaaaatga tatcgaaaga aaaatttctg aagctcatat caagcaagag
    35581 ttgcaacagc agctcaaaga agcaaatgat aaagcgcaaa aagataagat tcaagaagat
    35641 ttggaaaaac gtttagaaga aaataaactc attcataaag aaaaactaga acaaacactc
    35701 aaaaaagaag tggaaaaaat gcctgagaaa tttatcgaac aggttgagat aaaacgtgtg
    35761 gaacagttga aacaatcagc tcaagatgaa attcgtgacc atttacgagg gtttgcaaga
    35821 acaattccaa gttttattat ggcttacggt gatcaaactc taacacttga taattttgat
    35881 gcctttgttc ctgaacatgt tttttatgaa gtaacaggga ttacgattga tcagtttaga
    35941 tatttgcgag atggtgggca ggattttgca gggcatctct ttgataaagc aacatttgac
    36001 gaagctattc aagaatttct tcgcaagaaa aaggagttgg cggattattt taaagatcaa
    36061 aaagaagaca tttttgacta tattccaccg cagaagacca accaaatttt cactcctaaa
    36121 cgagtggtga aaaggatggt agatgatttg gaaaaggaaa atccagggat ttttgatgat
    36181 ccatctaaga cttttattga tttatatatg aagtcaggcc tctatattgc agaacttgtg
    36241 aagcggttat ataatagcaa tggcttgaaa gaggcctttc caaatcctga agaacgctta
    36301 aaacatattt tggaaaagca agtttatgga tttgctccgt ctgagattat ctataacatt
    36361 tccactaatt ttatatttgg caatctttct aaagatatca gtaggaagaa ttttgtttta
    36421 gcagatacca ttccagcggc taaagaaggg agcattcaaa agttggttga ttcctatttt
    36481 gaaaataatt aaaaagaagg ccgagtcaaa attctttgaa atcagaaaaa acgcataata
    36541 ttgagtgctt ttgtactgcc ccccaaaagt tagacagaaa aaatctaact tttggggggc
    36601 agttcagaca atccttggta ttatgcgttt tattgtggga agatgtataa tggattgaaa
    36661 taagatatga acaaatcaat taggaattta aagcatttta taacaacgtt ttagagtaat
    36721 ggggggctat ttcaacttca acctactata atacagaaaa aaacaactcc ctgataattc
    36781 aaggagttgt ctatagttaa attagttttt agaagcttct tggaattctg ggtttttcca
    36841 tgcttcgtca atgatagctt gtaattcttt agcagatgct tgcatttttt gagtttctgc
    36901 gtcgttcaat gggatattta ctggacgaac gataccatgt gcaccaacaa cagctggttg
    36961 accgataaag acattctcaa ctccgtattg accttcttgg aatactgaaa gtggaagtac
    37021 tgcgttttca tcgtcaagga ttgctttagt gatacgagca agggctactg cgataccgta
    37081 gtatgttgca ccttttttgt tgatgattgt gtaggctgca tcacgaacac cttcgaacaa
    37141 ttcaatcaat tcagcttctt gaacattttg agtgtcttta aggaattctt caaggtttac
    37201 accagcgatg ttagcgtgtg accaaacagc gaactcagag tcaccgtgtt cacccatgat
    37261 gtaggcgtgc actgaacgag catccacatc caatttttca gcaagtgctt gacggaaacg
    37321 agctgagtca agtgaagtac ctgaaccgat aacgcgttct ttagggaaac cagagaattt
    37381 ccaagttgag taagtcaaaa cgtcaactgg gttagcagca acaaggaaga tacctttgaa
    37441 accagattca acaacttgag ttacgattga tttgttgata gcaaggtttt tacctacaag
    37501 gtcaagacga gtttcacctg gtttttgagg tgcacctgca gtgatcacaa caaggtcagc
    37561 gtctgcacag tcagagtatt gagctgcata gattttttta ggtgaagtga aggcaagggc
    37621 gtgactaagg tcaagcgcat caccaacagc tttttcatgc aattgtggaa tttcgataat
    37681 tccaagctct tgtgcaattc cttggttaac aagtgcaaaa gcgtaagatg aacctacagc
    37741 accatcaccg acaaggataa cttttttgtg ttgtttagtt gaagtcattg ttttaaacat
    37801 ctccttaatt ttattagggg attttcccta gacaacttca ttctatcact tttaaaaaac
    37861 tttgtcacga atatgcctta tagttctcga tgtaaacgtt ttagtggttt agaggctgaa
    37921 atagatggga atttatggta taatgttgtt acttactaat tgtgaaatga ggcatttatt
    37981 aatgcaggat aaaaatttag tgaatgtcaa tctgacaaag gagatgaagg caagttttat
    38041 cgactacgcc atgagtgtta tcgtagcgcg agctcttcct gatgttcgag atggcttaaa
    38101 acctgttcac cgtcgcattc tctacggaat gaatgaattg ggtgtgaccc cagacaaacc
    38161 ccataaaaaa tctgctcgta ttacagggga tgtcatgggt aaatatcacc cacacgggga
    38221 ttcctctatt tatgaagcca tggtccgtat ggctcaatgg tggagctacc gttacatgct
    38281 tgtagatggt catgggaatt ttggttccat ggatggagat agtgctgccg ctcaacgtta
    38341 taccgaggca cgtatgagca agattgctct ggaaatgctt cgtgatatca acaaaaatac
    38401 agttgatttc gttgataact atgatgccaa tgaacgggaa cccttggtct tgccagcgcg
    38461 ttttccaaac cttttggtta atggagcaac tggtatcgcg gttgggatgg caaccaatat
    38521 tccacctcat aatctgggtg aaaccattga tgcagtgaag ttggtcatgg ataatcctga
    38581 agtgactacc aaggacttga tggaagtctt gcctggacca gattttccaa ctggtgctct
    38641 tgtcatgggg aaatcaggta tccataaggc ttatgaaaca ggtaaaggtt cgattgtcct
    38701 acgttctcgt acagagattg aaacgactaa gactggtcgt gagcgtatcg ttgtaacaga
    38761 atttccttac atggtcaata aaaccaaggt gcatgagcat attgttcgct tggttcagga
    38821 aaaacgcatt gagggtatca cagcagtacg tgatgagtca aaccgtgaag gtgttcgatt
    38881 tgttattgaa gtcaagcgcg acgcctcagc caatgttatt ctcaataacc tcttcaaaat
    38941 gacccaaatg caaaccaatt ttggtttcaa tatgctcgct atccaaaatg gtataccgaa
    39001 aattttgtct cttcgtcaga ttttggatgc ttatatcgag caccaaaaag aagtggttgt
    39061 tcgtcgtaca cgttttgata aggaaaaagc ggaagcgcgc gctcatatct tagaaggtct
    39121 cttgattgcg ctagaccata tcgacgaagt gattcgtatc atccgtgcta gtgaaacgga
    39181 tgcggaagct caagctgagt tgatgagcaa gtttaagctt tctgaacgtc aaagtcaagc
    39241 tatccttgat atgcgtcttc gtcgtttgac aggtttggaa cgcgataaga ttcaatctga
    39301 gtatgatgac ctcttggctc tgattgcgga tttagcagat attcttgcta agcctgaacg
    39361 tgtttctcaa attatcaaag acgaattgga tgaagttaaa cgtaaatttt ctgataagcg
    39421 ccgtacagag ttgatgattg gacaggtctt gagtctcgag gatgaggact tgattgaaga
    39481 atcggatgtc ttgattaccc tttctaacag aggctacatt aagcgtttgg atcaggacga
    39541 gttcactgct caaaaacgtg ggggtcgtgg tgtccaagga acgggagtga aagatgatga
    39601 ctttgttcgt gagttagtgt caactagcac ccatgatcat ctgctcttct tcacaaacaa
    39661 gggacgtgtc tatcgtctta aaggttatga aattcctgag tatggtcgga ctgccaaagg
    39721 gctaccagta gtcaatctct tgaaattgga tgaagacgaa agtattcaga cggttatcaa
    39781 tgttgagtct gatcgcagtg atgatgctta tctcttcttt acaacccgtc acggtattgt
    39841 gaagagaacc agtgttaagg agtttgccaa tattcgtcaa aatggtctca aagcgctgaa
    39901 tttaaaggat gaagatgagt taatcaatgt cttgttggca gaaggagata tggatattat
    39961 cattggtacc aagtttggtt atgcagttcg ctttaatcaa tcagccgttc gtggtatgag
    40021 tcgtatcgcc actggtgtga aaggtgttaa ccttcgtgaa ggagacacag ttgttggtgc
    40081 cagcttgatt actgatcaag atgaggttct tattatcaca gaaaaaggat atggtaagcg
    40141 tacagtcgct actgaatacc caacaaaagg tcgtggtggt aagggaatgc agacagctaa
    40201 aattaccgaa aaaaatggct tgctggccgg tcttatgact gttcaagggg atgaggattt
    40261 gatgattatc actgatacag gtgtcatgat tcgaaccaat cttgccaata tttcacaaac
    40321 aggacgtgca actatgggag ttaaagtaat gcgcctggat caagatgctc agatagtgac
    40381 tttcacaacg gttgcggtgg cagaaaaaga agaagttggg acagaaaacg aaacagaagg
    40441 tgaagcataa tgtctcaaaa aaataataaa aagaaaaaca agcgaaaaaa tctgctgaca
    40501 aatatcctag caggatttct gatattactg tcactggctt tgatttttaa tactcaaatt
    40561 cgaaatattt tcatagtctg gaataccaat aagtatcaag ttagccaggt atcaaaagaa
    40621 aaattagaag aaaatcagga tacagaaggc aattttgact ttgattctgt caaagctatc
    40681 tcttcggaag ctgttctaac ttctcaatgg gatgctcaaa aattaccagt tattggggga
    40741 attgcaattc ctgaattgga aatgaatttg ccgattttta aaggacttga taatgttaat
    40801 ctcttctacg gagctggtac aatgaaacgc gagcaagtaa tgggagaagg aaattatagt
    40861 ctagctagtc accatatctt tggtgttgat aatgctaata aaatgttatt ttctccttta
    40921 gataatgcta aaaatggcat gaagatttat ctaaccgata aaaataaagt ttatacttat
    40981 gaaatacgtg aagtcaaacg tgtgacaccg gatcgtgttg atgaagttga tgatagagat
    41041 ggggtcaatg aaatcacatt agtaacctgt gaagaccttg ctgctacaga acgtattatt
    41101 gtcaaaggtg atttgaaaga aacaaaagat tattcacaaa catctaatga aatcctaaca
    41161 gctttcaatc aaccatataa acaattttat taatacaaat cagtgaaatc catgatttca
    41221 ctgatttttt a
//
LOCUS       DAVHEX010000009        40234 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVHEX010000009 DAVHEX010000000
VERSION     DAVHEX010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 40234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40234
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.009"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            5..75
                     /locus_tag="VVL45_000492"
     tRNA            5..75
                     /locus_tag="VVL45_000492"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37..39,aa:Gly,seq:tcc)
     gene            110..183
                     /locus_tag="VVL45_000493"
     tRNA            110..183
                     /locus_tag="VVL45_000493"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:144..146,aa:Ile,seq:gat)
     gene            266..355
                     /locus_tag="VVL45_000494"
     tRNA            266..355
                     /locus_tag="VVL45_000494"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:302..304,aa:Ser,seq:tga)
     gene            366..439
                     /locus_tag="VVL45_000495"
     tRNA            366..439
                     /locus_tag="VVL45_000495"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:400..402,aa:Met,seq:cat)
     gene            443..515
                     /locus_tag="VVL45_000496"
     tRNA            443..515
                     /locus_tag="VVL45_000496"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:476..478,aa:Phe,seq:gaa)
     gene            528..608
                     /locus_tag="VVL45_000497"
     tRNA            528..608
                     /locus_tag="VVL45_000497"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:562..564,aa:Tyr,seq:gta)
     gene            613..683
                     /locus_tag="VVL45_000498"
     tRNA            613..683
                     /locus_tag="VVL45_000498"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:645..647,aa:Trp,seq:cca)
     gene            698..770
                     /locus_tag="VVL45_000499"
     tRNA            698..770
                     /locus_tag="VVL45_000499"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:731..733,aa:His,seq:gtg)
     gene            781..852
                     /locus_tag="VVL45_000500"
     tRNA            781..852
                     /locus_tag="VVL45_000500"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:813..815,aa:Gln,seq:ttg)
     gene            862..945
                     /locus_tag="VVL45_000501"
     tRNA            862..945
                     /locus_tag="VVL45_000501"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:896..898,aa:Leu,seq:caa)
     gene            1052..2329
                     /locus_tag="VVL45_000502"
     CDS             1052..2329
                     /locus_tag="VVL45_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEU4186722.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     MKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDETDFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATSEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            2405..3889
                     /locus_tag="VVL45_000503"
     CDS             2405..3889
                     /locus_tag="VVL45_000503"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEU4186723.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPDTYPKVDLNF
                     DKLKDASYQEVAKLVLSAFLDDFTVEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFANVPGT
                     EIIVFYPKDGVSKIQELQMTTQTGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGEKVNFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTTELMNALNTQGQYKLTDFDAEILDLFAAEYATEEETAAEIKRVCELDS
                     YIEDPHTAVASAVYKKYQSATGDVTKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLEIAPIRHKTTVAAADMQAAVEAYLGL"
     gene            3950..5230
                     /locus_tag="VVL45_000504"
     CDS             3950..5230
                     /locus_tag="VVL45_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU4186724.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTIVSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            5234..5854
                     /locus_tag="VVL45_000505"
     CDS             5234..5854
                     /locus_tag="VVL45_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU4186725.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQDLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            6020..7132
                     /locus_tag="VVL45_000506"
     CDS             6020..7132
                     /locus_tag="VVL45_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU4186726.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEVTKTVIASEEVAPST
                     GTSVPEEQTTETTRPVEEATPQETTPAEKQETQASPQAASAVEVTTTSSEAKEVASSN
                     GATAAVSTYQPEETKIISTTYEAPAAPDYAGLAVAKSENAGLQPQTAAFKEEIANLFG
                     ITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDKIAEYAIQNMASRGISYIIWKQRFY
                     APFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            7455..7880
                     /locus_tag="VVL45_000507"
     CDS             7455..7880
                     /locus_tag="VVL45_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEU4186727.1"
                     /translation="MLEIQDLLYQLRLSEQASTQLFEKRLGISLTRYQILLFLLEHSP
                     CNQMAVQERLKIDQAALTRHFKILETEGLVERHRNPENQREVLVEAAKYAKEQLVVNP
                     PLQHIRVKEEIESILTEFERTELSRLLNKLVLGIENIEI"
     gene            7891..8250
                     /locus_tag="VVL45_000508"
     CDS             7891..8250
                     /locus_tag="VVL45_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEU4186728.1"
                     /translation="MSIILTTIVALEHFYIFYLESIATQSDATSRVFNMEKEELAHPS
                     VSSLFKNQGIYKALLGVFLLYVIYFSQNLEIVTIFVLFVIGAATYGSLTADKKIILKQ
                     GGSAILALISILLFKYT"
     gene            8357..9001
                     /gene="pcp"
                     /locus_tag="VVL45_000509"
     CDS             8357..9001
                     /gene="pcp"
                     /locus_tag="VVL45_000509"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEU4186729.1"
                     /translation="MKVLVTGFEPFGGEKGNPALEAIKGLPAEIHGAEVRWLEVPTVF
                     HKSAQVLEEEMNRYQPDFVLCIGQAGGRTSLTPERVTINQDDACISDNEDNQPIDRPI
                     RPDGASAYFSSLPIKAMVQAIKKEGLPASVSNTAGTFVCSHLMYQALYLVEKKSPYVK
                     AGFMHIPYMMEQVVNRPTTPAMSLVDIRRGIEAAIGAIIEHGDQELKLVGGETH"
     gene            complement(9029..9886)
                     /locus_tag="VVL45_000510"
     CDS             complement(9029..9886)
                     /locus_tag="VVL45_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEU4186730.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSGFLNP
                     LSRIHGTVLEQPFFSILGQILQTYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            10016..11158
                     /gene="tgt"
                     /locus_tag="VVL45_000511"
     CDS             10016..11158
                     /gene="tgt"
                     /locus_tag="VVL45_000511"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEU4186731.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF"
     gene            11256..13073
                     /locus_tag="VVL45_000512"
     CDS             11256..13073
                     /locus_tag="VVL45_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEU4186732.1"
                     /translation="MRIKWFSLIRIIGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNNEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAVAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQESKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPIVQTLRPLPYIHAILAAGTGILTIIVCTVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            13226..14377
                     /gene="nagA"
                     /locus_tag="VVL45_000513"
     CDS             13226..14377
                     /gene="nagA"
                     /locus_tag="VVL45_000513"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEU4186733.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            14540..15598
                     /locus_tag="VVL45_000514"
     CDS             14540..15598
                     /locus_tag="VVL45_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEU4186734.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPEKAIKEIYDLTDGRGVDVAIEAVGIPATFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            15749..16114
                     /locus_tag="VVL45_000515"
     CDS             15749..16114
                     /locus_tag="VVL45_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEU4186735.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            16190..17131
                     /locus_tag="VVL45_000516"
     CDS             16190..17131
                     /locus_tag="VVL45_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEU4186736.1"
                     /translation="MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDHKIASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAS
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            17079..18095
                     /locus_tag="VVL45_000517"
     CDS             17079..18095
                     /locus_tag="VVL45_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU4186737.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSALLDKQCVTQMRVGLSQGKSFSEMMESLGCSSAIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEVGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            18097..18423
                     /gene="comGC"
                     /locus_tag="VVL45_000518"
     CDS             18097..18423
                     /gene="comGC"
                     /locus_tag="VVL45_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="comG operon protein ComGC"
                     /protein_id="HEU4186738.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLEKNEDASLRKLQADGRITEEQAKAYKEYNDKNGGA
                     NRKVND"
     gene            18416..18820
                     /locus_tag="VVL45_000519"
     CDS             18416..18820
                     /locus_tag="VVL45_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEU4186739.1"
                     /translation="MIKAFTMLESLLVLGLVSILALGLSGSVQSTFSAVEEQIFFMEF
                     EELYRETQKRSVASQQKTSLNLDGQTLSNGSQKLPVPKGIQAPSGQSITFDRAGGNSS
                     LAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            18825..19085
                     /locus_tag="VVL45_000520"
     CDS             18825..19085
                     /locus_tag="VVL45_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4186740.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            19048..19509
                     /locus_tag="VVL45_000521"
     CDS             19048..19509
                     /locus_tag="VVL45_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEU4186741.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEVELDRSQFEKVEGNRLYMKQDGKDIAIGKSKSDDFR
                     KTNARGRGYQPMVYGLKSVRITEDNQLVRFHFQFQKGLEREFIYRVEKEKS"
     gene            19487..19900
                     /locus_tag="VVL45_000522"
     CDS             19487..19900
                     /locus_tag="VVL45_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEU4186742.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLNRQVAHYQDYALNK
                     EKLVAFAMAKRTKDKVEQESGEQFFNLGQVSYQNKKTGLVTRVRTDKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKVEKKKSEEKPEKKENS"
     gene            20037..20631
                     /locus_tag="VVL45_000523"
                     /pseudo
     CDS             20037..20631
                     /locus_tag="VVL45_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            20692..21645
                     /locus_tag="VVL45_000524"
     CDS             20692..21645
                     /locus_tag="VVL45_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4186743.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKENNQTLKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGAIFLTSLTKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGL
                     KYLKSDGYAIFLAPSDLLTSPQSDLLKEWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKNEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            21696..22886
                     /locus_tag="VVL45_000525"
     CDS             21696..22886
                     /locus_tag="VVL45_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEU4186744.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHIQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGADAIVFTAGVGE
                     NAESFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            23035..23406
                     /gene="rnpA"
                     /locus_tag="VVL45_000526"
     CDS             23035..23406
                     /gene="rnpA"
                     /locus_tag="VVL45_000526"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEU4186745.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNRFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            23381..24205
                     /locus_tag="VVL45_000527"
     CDS             23381..24205
                     /locus_tag="VVL45_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU4186746.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAEIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            24224..25210
                     /locus_tag="VVL45_000528"
     CDS             24224..25210
                     /locus_tag="VVL45_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEU4186747.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QAESQELEDLGLKVETNFDIEQVATEVMAYVQTIIDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNRYSRTFYVTINVNDYVEHRAEVLQTYAQKL
                     ATRVLEEGRSHKTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            25261..25884
                     /locus_tag="VVL45_000529"
     CDS             25261..25884
                     /locus_tag="VVL45_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEU4186748.1"
                     /translation="MLNKIRDYLDFAGLQYRNPDKAGAEREKMLAFRHKGQEARKVFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQRLRPHFWVYLQRDGQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQAKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            26015..27472
                     /locus_tag="VVL45_000530"
     CDS             26015..27472
                     /locus_tag="VVL45_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU4186749.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIVAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            27495..27776
                     /locus_tag="VVL45_000531"
     CDS             27495..27776
                     /locus_tag="VVL45_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU4186750.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            27852..28337
                     /locus_tag="VVL45_000532"
     CDS             27852..28337
                     /locus_tag="VVL45_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU4186751.1"
                     /translation="MNLKQALIDNDSIRLGLEANNWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            28354..29019
                     /locus_tag="VVL45_000533"
     CDS             28354..29019
                     /locus_tag="VVL45_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEU4186752.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGIDVFTFIAGRGITEAVDPAGAARAFKDEIKR
                     IWG"
     gene            29023..29886
                     /locus_tag="VVL45_000534"
     CDS             29023..29886
                     /locus_tag="VVL45_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEU4186753.1"
                     /translation="MVRPIGIYEKATPTHCTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKAIYETGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIS
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SKGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            29888..30592
                     /locus_tag="VVL45_000535"
     CDS             29888..30592
                     /locus_tag="VVL45_000535"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEU4186754.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            30769..32442
                     /locus_tag="VVL45_000536"
     CDS             30769..32442
                     /locus_tag="VVL45_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU4186755.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALVDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            32554..33645
                     /gene="ulaG"
                     /locus_tag="VVL45_000537"
     CDS             32554..33645
                     /gene="ulaG"
                     /locus_tag="VVL45_000537"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEU4186756.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNSKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            33759..35735
                     /gene="tkt"
                     /locus_tag="VVL45_000538"
     CDS             33759..35735
                     /gene="tkt"
                     /locus_tag="VVL45_000538"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU4186757.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDTNRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVISMPSTDVFDKQNAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKVVRNLK"
     gene            35852..36151
                     /gene="yajC"
                     /locus_tag="VVL45_000539"
     CDS             35852..36151
                     /gene="yajC"
                     /locus_tag="VVL45_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU4186758.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            36197..36625
                     /locus_tag="VVL45_000540"
     CDS             36197..36625
                     /locus_tag="VVL45_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEU4186759.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            36627..37037
                     /locus_tag="VVL45_000541"
     CDS             36627..37037
                     /locus_tag="VVL45_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4186760.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            37337..39988
                     /gene="adhE"
                     /locus_tag="VVL45_000542"
                     /gene_synonym="adhC"
     CDS             37337..39988
                     /gene="adhE"
                     /locus_tag="VVL45_000542"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEU4186761.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELSFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT        12197 a         7212 c         9076 g        11749 t
ORIGIN      
        1 tttagcgggt gtagtttagt ggtaaaacta cagccttcca agctgttgtc gcgagttcga
       61 ttctcgtcac ccgctttgaa ctttgttctt tgtaccaagt ttttgacttg ggcgcgtagc
      121 tcaggtggtt agagcgcacg cctgataagc gtgaggtcgg tggttcgagt ccactcgtgc
      181 ccatagtgtt tagtccatta ctaggggatt ggaatattat ctgttcacta agaggacacg
      241 ggcttgttcc cgtataaact attttggagg attacccaag tccggctgaa gggaacggtc
      301 ttgaaaaccg tcaggcgtgt aaaagcgtgc gtgggttcga atcccacatc ctccttttat
      361 attaacgcgg gatggagcag ctcggtagct cgtcgggctc ataacccgaa ggtcgtaggt
      421 tcaaatcctg ctcccgcaat aaggctcggt agctcagttg gtagagcaat ggattgaagc
      481 tccatgtgtc ggcggttcga ttccgtctcg cgccatttat atattttgga agggtagcga
      541 agaggctaaa cgcggcggac tgtaaatccg ctccttcggg ttcgggggtt cgaatccctc
      601 cccttccatt ttacgggcat agtttaaagg tagaactaag gtctccaaaa ccttcagtgt
      661 gggttcaatt cctactgccc gtgttaatag aattatggcg ggtgtggtga agtggttaac
      721 acaccagatt gtggctctgg catgcgtggg ttcgatcccc atcactcgcc tattttatat
      781 tggggtatag ccaagcggta aggcaaggga ctttgactcc ctcatgcgtt ggttcgaatc
      841 cagctacccc agttactatt tgccggcgtg gcggaattgg cagacgcgct ggactcaaaa
      901 tccagtgtcc gcaaggacgt gccggttcga ccccggccgc cggtatagta tagtgttagg
      961 aacgttgtta ttcttcgttc cttttttata ttatttttgg tataattata gttattcaaa
     1021 ttttatttag attaagaaag tgtaggggag tatgtcttgt tctatcgatt tattaaaaca
     1081 tcggtatttg aaaaatatta aagaaaatcc tgaattgttt gtcggaattg agttggagta
     1141 tcctgttgca agtttagaag gggatgctac agatgttgaa gttatgaagg atctatttca
     1201 ttatttagtt tctactttgg atctcaccgt agcaaaggta gatgattttg gcaatctgat
     1261 ccagttagta gatccgataa gtcaggatgc tattttattt gaagtttcct atacaacgat
     1321 tgagtttgca tttggtaagg ctgaaacgat tcaagaggtc gaaaatcgtt tcaataatta
     1381 tatgaatgta attcagagaa agttagctga atcaaatcat gctattgttg gctgtggtat
     1441 ccatcccaac tgggataaaa atgagaattg tccagtggct tatccacgct atcagatgtt
     1501 gatggattat ttgaatttga gtagaaatat tattaaatca gatttacatc atttccctga
     1561 atatggtact tttatctgtg ggagccaggt tcagctggat atttcaaaaa ccaactactt
     1621 acgggtgatt aatgctttta ctcaaattga agcggctaag gcttatttat ttgcaaactc
     1681 tgaattttcg ggtgcggatt gggatacgaa aatttcaagg gatattttct gggaagaatc
     1741 tatgcatggt atctatccag agaatgttgg ggtcaatgct agactcctta atgatgaaac
     1801 tgattttttt gactatctaa atcattctgc gatttttact gcggaacgtg atgggcagac
     1861 ctattatttt tatcctattc aggctgggga ctatttggct acgtccgaaa tccaagcatt
     1921 tgctctgaat ggggatgagg ttattattta cccccaagag aaggattttg aaactcatcg
     1981 tagttaccag taccaagatt taacgactcg aggaacagtt gagtttcgta gtgtgtgtac
     2041 acagccactt gataggactt ttgcttctgc agcttttcac ttgggattat tggttaattt
     2101 agacaagtta gaagcttact tagaaacagc acctttcttt aaagtatttg gttatgatta
     2161 caagtcttta aggagacaat tttctaagaa aaatcttaca gatgaggaag aaactacgat
     2221 tattgaattt tccaaagact tactcctact agctgaggag ggactagtgg tgagaaataa
     2281 ggaagaaatg acctatttac agcctttgag agaagaattg agcctataat ttctcttata
     2341 aagggagaat tttctgaaaa atcatgatat aatggacgag actatagata aaggatagag
     2401 agtaatgaca ttagtttatc aatcaacgcg tgatgccaac aatacagtaa ctgccagcca
     2461 agcaattttg caaggtttgg cgacggacgg cggtttgttt acaccggata cttatccaaa
     2521 ggtagatttg aactttgaca aattgaaaga tgcttcttac caggaagttg ctaagctagt
     2581 tttgtcagca tttttagatg actttacagt tgaggagttg gactactgta tcaacaatgc
     2641 ctacgatagc aaatttgata ctccagctat tgcaccatta gtgaaattag atgggcaata
     2701 caatttggaa cttttccatg gttcaacgat tgcctttaag gatatggcct tgtctatttt
     2761 gccatacttt atgacgactg ctgctaagaa acatggtttg gagaacaaga ttgttatctt
     2821 gacagcgaca tctggtgaca cggggaaagc tgctatggcg gggtttgcga atgtgcctgg
     2881 tactgagatt atcgtctttt atccaaagga tggtgtcagc aagattcaag agttacaaat
     2941 gaccactcag actggcgaca atactcatgt tattgctatt gatggtaact ttgacgatgc
     3001 gcaaacaaat gtgaagcaca tgtttaacga cgtggctctt cgtgaaaaat tgactaccaa
     3061 caagttgcaa ttttcatcag ctaactctat gaacattggt cgtctggtgc cacaaattgt
     3121 ttattatgtt tatgcttacg ctcaattggt taagactggt gaaattgtag ctggtgaaaa
     3181 ggttaacttc acagtaccaa caggaaactt tggaaatatc ttggctgcct tttatgccaa
     3241 acaaattggt ttgccagttg gtaaattaat ctgtgcttca aatgacaaca atgttttgac
     3301 agacttcttt aaaacacgtg tctatgacaa aaaacgtgag tttaaggtaa caaccagccc
     3361 atctatggat atcttggtat cttcaaactt ggagcgcttg attttccatc ttttgggaaa
     3421 taatgctgaa aagacaactg aacttatgaa tgccttgaac acgcaaggac aatataagtt
     3481 gacagacttt gatgcagaga ttttggacct ctttgcagct gaatatgcga ctgaggaaga
     3541 aacggcagca gagatcaagc gtgtttgtga gttagattct tatatcgagg accctcatac
     3601 agctgttgct tcagcagttt ataaaaaata ccaatcggcc actggagatg taactaagac
     3661 agtgattgct tcaacagcta gtccatacaa gttcccagta gttgcagtag aagctgtaac
     3721 tggaaaagca ggtttaacag actttgaagc cttggctcaa ttacatgaaa tctcaggcgt
     3781 tgcagtgcca ccagcagttg atgggcttga aatagctcca attcgtcaca agacaacagt
     3841 ggcagctgct gacatgcaag cagcggttga ggcttattta ggactttaag acagagggag
     3901 caaactcggt tgggaaacca actgagtttc ttttcatcag gaggagagat tgtttaagaa
     3961 aaataaagac attcttaata ttgcattgcc agctatgggt gaaaactttt tgcagatgct
     4021 aatgggaatg gtggacagtt atttggttgc tcatttagga ttgatagcta tttcaggggt
     4081 ttcagtagct ggtaatatta tcaccattta tcaggcgatt ttcatcgctc tgggagctgc
     4141 tatttccagt gttatttcaa aaagcatagg gcagaaagac cagtcgaagt tggcctatca
     4201 tgtgactgag gcgttgaaga ttaccttact attaagtttc cttttaggat ttttgtccat
     4261 cttcgctggg aaagagatga taggactttt ggggacggag agggatgtag ctgagagtgg
     4321 tggactgtat ctatctttgg taggcggatc gattgttctc ttaggtttaa tgactagtct
     4381 aggagccttg attcgtgcaa cgcataatcc acgtctgcct ctctatgtta gttttttatc
     4441 caatgccttg aatattcttt tttcaagtct agctattttt gttctggata tggggatagc
     4501 tggtgttgct tgggggacaa ttgtgtctcg tttggttggt cttgtgattt tgtggtcaca
     4561 attaaaactg ccttatggga agccaacttt tggtttagat aaggaactgt tgaccttggc
     4621 tttaccagca gctggagagc gacttatgat gagggctgga gatgtagtga tcattgcctt
     4681 ggtcgtttct tttgggacgg aggcagttgc tgggaatgca atcggagaag tcttgaccca
     4741 gtttaactat atgcctgcct ttggcgtcgc tacggcaacg gtcatgctgt tggcccgagc
     4801 agttggagag gatgattgga aaagagttgc tagtttgagt aaacaaacct tttggctttc
     4861 tctgttcctc atgttgcccc tgtcctttag tatatatgtc ttgggtgtac cattaactca
     4921 tctctatacg actgattctc tagcggtgga ggctagtgtt ctagtgacac tgttttcact
     4981 acttgggacc cctatgacga caggaacagt catctatacg gcagtctggc agggattagg
     5041 aaatgcacgc ctcccttttt atgcgacaag tataggaatg tggtgtatcc gcattgggac
     5101 aggatatctg atggggattg tgcttggttg gggcttgcct ggtatttggg cagggtctct
     5161 cttggataat ggttttcgct ggttatttct acgctatcgt taccagcgct atatgagctt
     5221 gaaaggatag gaaatgcaaa aaacagcttt tatttgggat ttagacggga ctttattgga
     5281 ctcttacgaa gcgattttat cagggattga ggagactttt gctcagtttt ctattcctta
     5341 tgataaggag aaggtgagag agtttatctt caagtattcg gtgcaagatt tgcttgtgcg
     5401 ggtggcagaa gatagaaatc tggatgttga ggtgctaaat caggtgcgtg cccagagtct
     5461 ggctgagaag aatgctcagg tagttttgat gccaggtgcg cgtgaggtgc tagcttgggc
     5521 agacgaatca ggaattcagc agtttatata tactcataag gggaacaacg cttttaccat
     5581 tctcaaggac ttgggggtgg aatcctattt tacagagatt ttaaccagtc agagtggctt
     5641 tgtgcggaag ccaagtccag aagcggctac ctatctgcta gataagtatc agttgaattc
     5701 tgataatact tattatatag gggatcggac tctggatgtg gaatttgccc agaatagtgg
     5761 gattcaaagc atcaactttt tagagtctac ttatgaaggg aatcacagga ttcaagcgtt
     5821 agcagatatt tcccgtattt ttgagactaa gtgataaaaa gattgtgtca gttttgtgac
     5881 agagacctaa caaactattt caagtaacct agtttgttac aaggaataga cagttctgtt
     5941 aaataggccc gagagggctt tttttctaca ttttttgtgt tatgatagac aggtactcat
     6001 ttgaaaggaa tttgaaagaa tgaagaaaag aatgttatta gcgtcaacag tagccttgtc
     6061 atttgcccca gtattggcaa ctcaagcaga agaagttctt tggactgcac gtagtgttga
     6121 gcaaatccaa aacgatttga ctaaaacgga caacaaaaca agttataccg tacagtatgg
     6181 tgatactttg agcaccattg cagaagcctt gggtgtagat gtcacagtgc ttgcgaatct
     6241 gaacaaaatc actaatatgg acttgatttt cccagaaact gttttgacaa cgactgtcaa
     6301 tgaagcagaa gaagtaacag aagttgaaat ccaaacacct caagcagact ctagtgaaga
     6361 agtgacaact gcgacagcag atttgaccac taatcaagtg accgttgatg atcaaactgt
     6421 tcaggttgca gacctttctc aaccaattgc agaagttaca aagacagtga ttgcttctga
     6481 agaagtggca ccatctacgg gcacttctgt cccagaggag caaacgaccg aaacaactcg
     6541 cccagttgaa gaagcaactc ctcaggaaac gactccagct gagaagcagg aaacacaagc
     6601 aagccctcaa gctgcatcag cagtggaagt aactacaaca agttcagaag caaaagaagt
     6661 agcatcatca aatggagcta cagcagcagt ttctacttat caaccagaag agacgaaaat
     6721 aatttcaaca acttacgagg ctccagctgc gcccgattat gctggacttg cagtagcaaa
     6781 atctgaaaat gcaggtcttc aaccacaaac agctgccttt aaagaagaaa ttgctaactt
     6841 gtttggcatt acatccttta gtggttatcg tccaggagac agtggagatc acggaaaagg
     6901 tttggctatc gactttatgg taccagaacg ttcagaatta ggggataaga ttgcggaata
     6961 tgctattcaa aatatggcca gccgtggcat tagttacatc atctggaaac aacgtttcta
     7021 tgctccattc gatagcaaat atgggccagc taacacttgg aacccaatgc cagaccgtgg
     7081 tagtgtgaca gaaaatcact atgatcacgt tcacgtttca atgaatggat aaacccgact
     7141 tgataacatc attttgacga atgagatcta gctttcgtga tggaaagcga ttctcgttcg
     7201 ttttttcttt gtcatactct tcgaaaatct cttcaaacca cgtcagtttt atctgaaact
     7261 tcaaagctgt gctttgagca acctgcgact agcttcctag tttgcttttt gattttcatt
     7321 gagtatcaat ttgaatggaa aatggaaagt tatcatcttg taatgagtta agcaacattc
     7381 ttgcaatcta ttttacttta tatcacaatt aattagtcaa atattgataa atcaataaaa
     7441 agagagggga agaaatgcta gagattcaag atttactgta tcaactccgc ttgtctgagc
     7501 aagcgagtac gcaattgttt gaaaaaaggc ttgggattag tttgacacgg tatcagattt
     7561 tactgttttt gctggagcat tctccttgta accaaatggc ggttcaggag cgtttgaaaa
     7621 ttgatcaggc tgctttgaca cggcatttca aaattttgga aacggaaggt ttggtggagc
     7681 gtcatcgtaa tcctgaaaat cagcgggaag tgttggtaga ggctgcgaag tatgccaagg
     7741 agcagttagt ggtgaatccc cctctgcaac atatcagggt taaggaagag atagaaagta
     7801 tcttaacaga gtttgagaga acagaactca gccgtttatt aaataaattg gttttgggta
     7861 ttgaaaatat agaaatttaa ggagaaatag atgtcaatta ttttaacaac gatcgttgct
     7921 ttggagcatt tttacatttt ttatttggaa agtattgcca cgcaatcaga tgcgactagt
     7981 cgtgtattta atatggaaaa ggaagaattg gctcatccgt cagtaagttc attgttcaaa
     8041 aatcaaggaa tttataaggc tctgctagga gtctttctct tgtatgtcat ttatttctca
     8101 cagaatttag aaattgtgac tatttttgtc ttatttgtga ttggtgctgc gacttacggc
     8161 tctttaacag cggataaaaa aattattttg aaacaaggtg gatcagctat tttggccttg
     8221 attagtattt tactctttaa atacacttga aggtcgattc taatctcgct aatccttttt
     8281 aatccagaat aagggaaata tgttatactt gtttttaaga aaaaagtctc attgaattgg
     8341 ttttgaggag ttagaaatga aagtattagt gacaggtttt gagccctttg gaggggaaaa
     8401 gggcaatcca gctttggagg ccattaaagg tttaccagct gaaatccatg gtgctgaggt
     8461 ccgttggcta gaggtgccga cagtttttca caaatctgct caagtattgg aagaagagat
     8521 gaatcgttat caacctgact ttgtcctttg tattgggcaa gctggtggaa gaactagttt
     8581 gacacctgaa cgagtgacca ttaatcaaga cgatgcatgc atttctgata acgaagataa
     8641 tcaaccgatt gaccgtccca ttcgcccaga tggtgcttcg gcctacttta gtagtttgcc
     8701 gattaaagcg atggttcaag ctataaaaaa agagggctta ccggcctctg tttccaatac
     8761 ggcagggact tttgtctgca gccatttgat gtatcaggct ctctatttgg tagaaaagaa
     8821 atctccatat gttaaggcag gttttatgca tattccttat atgatggaac aggtggtgaa
     8881 cagaccgact actccagcta tgagtttagt ggatattcgg cgagggatag aagcagcaat
     8941 cggcgctata atagaacatg gagatcagga actcaagttg gtaggcggag aaactcattg
     9001 atagaaaaaa gcttgagggg aaaaaccttc aagcttttgg acgttttcgg gccaatactg
     9061 ctcggtaaaa cataatttta gtgcattgga tataaggtag gagtgaaaaa ctagcaatgc
     9121 caaaggtaat ccaattgagg aagtaccaag gaagaagctg taaatctagg acaaagtgct
     9181 ggaacttgta gcccttcata aaggaacggc tagtttttag gattcgtctt ggtgggacct
     9241 gtcctaggtc tagactataa cagagaagaa attccacctg tgaataggca taatactgtg
     9301 gaatatagag gatatttcct acaatgatca agatgagact tgcaagaaag tagagtccaa
     9361 agaccatgag gaaacgctcg gtttcaactg atgagagatc tagatttgga aactcaggat
     9421 gtagggtgac gaattttttg gctaaaaagc tactataaaa gaggaggtaa atcccaagta
     9481 aattagggat actccataaa aagagataga aacgtttgag aagtagggtc aaaaaggttt
     9541 gagaaaagcg ctcctcatca aagagagcta ggctgttttt tacagatggc tccgttttag
     9601 aatctttcat gagtgtcagt gttgcataga cggaactggt caaaagaata gtcccgataa
     9661 aggagactag tagaggaaag aggtaggttt gaagtatttg gccaagtatg ctgaaaaatg
     9721 gctgttctaa aacagtcccg tggatccgag ataagggatt aagaaaacca gataagatga
     9781 ccagcatact gggaaggata tagaggagaa agagacgggg ggtgtcagcc tgaaaatgtt
     9841 ttgactcctg acgaattgtt tttaaatcaa tttttggata gttcattctc ttattatacc
     9901 atagttctta tacatagttc gtgacagttc ctactttttt tgataaaatc atacagtgtg
     9961 tccttgggca cactgtatga actgggactg tctttcccag cttcggaggt aaaaaatgtc
    10021 agattcacca atcaaatatc gtttgattaa gaaagaaaaa cacacaggag ctcgtctggg
    10081 agaaatcatc actccccacg gtacctttcc gacacctatg tttatgccag ttgggacaca
    10141 agccactgtc aaaactcagt cacctgaaga attgaaggag atgggttcgg gaattatcct
    10201 atcaaacacc tatcatctct ggcttcgccc tggagatgaa ctcattgcac gcgctggtgg
    10261 tctccacaag ttcatgaatt gggaccagcc tatcttgaca gatagtggtg gttttcaggt
    10321 ttattcttta gcagatagcc gtaatatcac agaagaagga gtaaccttta aaaatcatct
    10381 aaatggttct aagatgttcc tatccccaga aaaagccatc tctattcaga ataatctggg
    10441 ttcagacatc atgatgtcct ttgatgaatg tcctcagttt tatcaacctt atgactacgt
    10501 taagaaatcg atcgagcgta ccagccgttg ggctgagcgt ggtttgaagg ctcaccgtcg
    10561 tccacatgac caaggtttgt ttggaattgt gcaaggtgca ggatttgaag accttcgccg
    10621 ccaatcagct catgatcttg tcagcatgga tttctcaggc tactctatcg gtggtttggc
    10681 agtgggagaa acccatgaag agatgaatgc ggtcttggac tttacaactc aactgctgcc
    10741 tgaaaataaa cctcgttatc tgatgggtgt gggagcgcca gatagcttga tcgatggggt
    10801 cattcgtggg gtggatatgt ttgactgtgt cttaccgact cgaattgctc gtaacgggac
    10861 ttgtatgacc agtcaaggac gtttggttgt gaaaaatgcc cagtttgctg aggactttac
    10921 gccactggat cctgagtgtg attgctacac atgtaataac tatacacgcg cttaccttcg
    10981 tcacctgctc aaggctgatg aaacctttgg tatccgcttg actagctacc acaatcttta
    11041 cttcttgctt aacctgatga agcaagtgcg acaagccatc atggatgaca atctcttgga
    11101 attccgtgag tattttgtgg aaaaatatgg ctataataag tcaggacgta atttctaaaa
    11161 tggaattgat ataaaaaaat cctaagtttt ctcttaggat ttttcttctt tttttgatag
    11221 aataaagtgt acaatgaaag gaagaataaa ctcgtatgcg cattaaatgg ttttccttga
    11281 ttaggattat aggtttactt ttggtactct tgtatcactt ctttcagacg atctttcctg
    11341 gaggattttt cggggtagat gtctttttca cattttcagg tttcctgatt acagctctac
    11401 tcattgaaga attttctaag aacaatgaga ttgatttgat aggatttttt agaagacgct
    11461 tttatcggat tgtgccacct gtggttttga tggtcttggt gaccatgcct tttactttct
    11521 tggttcgcca agactatgtt gctggaattg gtggccagat tgcgggcgtc ttaggcttta
    11581 tgaccaactt ctatgaactc ctaacaggtg ggagttatga atctcagttc attcctcatt
    11641 tgtttgttca taattggagt ttggcagttg aggttcacta ctatattctt tggggattgg
    11701 cagtttggtt cttatccaaa caagctaaat caaatggtca gttgaagggg atggtctttc
    11761 tcttatctgc tgttgccttc ttgatcagtt tcttctccat gtttattggt agttttctag
    11821 tgacctctta ttcctctgtt tatttctcca gtttaactca tgtctatcca ttctttttag
    11881 gaagtatgtt agcaactatt gtaggcgttc gtcagacgac ttccctcgtc aagcagttgg
    11941 ataaaatctg ggatttacga aagactttgg tagtttttgg aggaggcttt ggtttcctag
    12001 ttcttttgac tttctttgtc aaattcactt atctttttgc ctatcttatc ggcttcttac
    12061 ttgccagtct tgcagctctt gccatgattc tggcggcgcg tgtcttacat gaaaagacac
    12121 atcatataca ggagtcgaag attatcagct ttttagcgga tactagctat gcggtttatc
    12181 ttttccattg gcctttctat atcattttct cacagttgac atcaaatctt cttgctgtat
    12241 tactgactct gatttgttct tatggctttg ccagtctgtc attttatgta ttggaacctt
    12301 ggattgcagg caagaacaca cctattgtcc aaacccttcg tcccctgcct tatattcacg
    12361 caattcttgc agcaggtaca ggaatcttga ccatcattgt ctgcacggtg accttgttgg
    12421 caccacaagt gggagcgttt gagacagact tgactgtcaa tggcttgaag caagctgcaa
    12481 caaatattgg ccagaccaag gtgatggcag aacgggcaga tgcaaacagt ttgggaattg
    12541 ctgatggcac tatgttaatt ggtgactcag tggctttaag ggcaaataca gcactacaga
    12601 cagctcttcc tggagcacag attaacgcgc aggtcagcgt aacaaccaag accgcaaatg
    12661 aaatcatgct aaataatagc cagaataaat ttttacctaa gacggtggtc attgcgactg
    12721 gggtaaataa tcctgagaat tacaaggatg actgggacag tatcgtgaaa aatcttccta
    12781 agggacacca tatgattttg gtgactcctt atgagggaga taagacaaaa gagacctatg
    12841 ccatcgttga gaaggctgct gcctatatga gagaattggc agagaagaca ccttacatta
    12901 cgatagcaga ttggaatcaa gttgcgaaag agcatccaga aatttgggct ggaacagacc
    12961 aggttcattt cgggagtgag agtagcacta tcgaagcagg agcaaaattg tatgcagata
    13021 cgattgccac agctttgcag acagctcaag acaagccggt taaatcaaaa taacttgtat
    13081 taaaaaagag aagagttgga gaaatccagc tcttttaaaa tatctccaaa aaataggtct
    13141 ataccattta caaataaaaa agaaaggttt ataatgtaat tgacataata aattgtagaa
    13201 tcaatctttt aaggaggtta acattatgcc taactatatt aaagcggatc agtttttcta
    13261 cccacacgga gttcgtcgag gtggttactt ggaacttgtg gacggcaagt ttgggaaaca
    13321 tgtagagcag attcctgaag gggctgaggt gattgactat acaggttata gcattgcccc
    13381 aggtcttgtg gatactcata ttcatggata tgcaggtgta gatgtgatgg acaacaacat
    13441 tgaaggtaca ttgcatacta tgagtgaagg acttcttagt accggtgtta ccagtttctt
    13501 acccacaact ttaacagcca cttatgagca attgcttgca gtcactgaaa atcttggaaa
    13561 ccattataaa gaagcaacag gtgctaagat tcgtgggatt tattatgaag gtccatattt
    13621 cacagaaact tttaaggggg cacaaaatcc aacttatatg agagacccgg gtgttgagga
    13681 gtttcattct tggcaaaaag cggcaaatgg cttgcttaat aaaattgccc ttgcaccaga
    13741 acgtgatggg gtggaagact ttgttcgtac agttacgggc gaaggtgtga cggttgctct
    13801 tggacattca aacgcgactt ttgatgaagc caaaaaagca gtcgatgctg gagcgagtgt
    13861 ttgggtgcat gcctacaatg gaatgcgtgg gttgactcac cgtgaattgg ggatggttgg
    13921 agccatgtac caattgccac atacctatgc agagttgatc tgtgatggtc accacgtaga
    13981 tccaaaggct tgcgaaattc ttatcaaaca aaaaggaaca gaaaacatcg ctcttatcac
    14041 agactgtatg acagctgggg gattggaaga cggagattat atgttgggag aattcccagt
    14101 tgtcgttgca aatggaactg cacgcctcaa atcgacaggt aacttggcag gttctatcct
    14161 caaactcaaa gatggtttga agaatgtggt cgaatgggga attgcgaatc cgcatgaagc
    14221 agtcatgatg gccagcttca acccagctaa atccgttcac atcgatgacg tctgtggcca
    14281 aatccgtgaa ggctacgacg ctgacttcat cgtattagat aaagatttgg aattggtagc
    14341 aacctaccta gatggcgtaa aacgttatca agcataagag agaaagcaga agttagagac
    14401 taacttctgc ttttttatgc agggtacttt actggtaaat aaaaagttaa aaaaatcaca
    14461 aaaaaagctt gaagaaacaa agaaaaaggc ttacaattga actataaata ttacaaataa
    14521 aaaaacggag gagtgcttta tgaaagccta tacttatgtt aaaccaggac ttgcttcttt
    14581 tgttgatgta gacaaaccag ttattcgcaa gccaacagac gctattgtgc gtattgtaaa
    14641 aaccactatt tgtggaacag acctccatat tatcaaaggg gatgttccta cttgccaaag
    14701 tggtaccatt cttggccacg aagggattgg gattgttgaa gaagttgggg aaggagtttc
    14761 caacttcaaa aaaggtgaca aggtcttgat ttcttgcgtc tgtgcctgtg gtaaatgcta
    14821 ctactgtaaa aaaggaattt atgctcactg tgaagacgaa gggggctgga ttttcggtca
    14881 cttgattgat ggtatgcagg ctgaatatct acgtgtccct catgcagata atactcttta
    14941 ccatactcca gaagacttgt cagatgaagc tttggttatg ctgtcagaca ttctgcctac
    15001 tggatatgaa attggtgtct taaaagggaa agtagaacct ggttgcagcg tagccattat
    15061 tggttcaggt ccagttggat tggctgctct tttaacagcc caattctatt caccagctaa
    15121 attgattatg gtagacctag acgataaccg cttggaaact gccctatcat tcggtgcgac
    15181 tcataaggtt aattcttcag accctgaaaa agccattaaa gaaatttatg atttgacaga
    15241 tggtcgtggt gtggatgtcg ctatcgaagc tgttggtatt cctgcaacat ttgatttctg
    15301 tcaaaagatt atcggtgtag acggaacggt tgccaactgt ggtgtgcatg gtaaaccagt
    15361 tgaattcgat ttagataaac tttggattcg caacatcaat gtaacaactg gtttggtatc
    15421 tacaaatacg actccacaat tgttgaaagc acttgaaagt cataagattg aaccggaaaa
    15481 attggtaact cactatttca aactcagtga aattgaaaaa gcctacgaag tcttcagtaa
    15541 ggcagcagac caccatgcca ttaaggtcat tatcgaaaac gatatctcag aagcctaagt
    15601 agtaaaaata tttttgtaca taagtaaata gaaattcagt catccatcag atggctggat
    15661 tttttatcaa aaaattaaga aatgagcata tttctttcct tgtctggcgg aattggttat
    15721 aatatacggt acaaaggaat gaatgaatat gtatcgtgtt atagaaatgt acggagattt
    15781 tgaaccgtgg tggttcttag aaggttggga agaagatatt gtagcaagta gaaaatttga
    15841 ccagtattat gatgctctca aatactacaa aacttgctgg tttagattgg aacaagaatc
    15901 gcctctttat aaaagtagaa gcgacttgat gaccattttt tgggacccgg aagaccaacg
    15961 ctggtgtgat gaatgtgatg agtatttaca acaataccat tctttggctc ttttgcagga
    16021 tgagcaggtt atcccagacg aaaaactacg ctcaggctat gaaaaacaaa ccagtcagga
    16081 aaggaatcgt tcttgccgta tgaaattaaa atagagaaaa gtaacttttt tggagttgct
    16141 ttttttattt ttctaactct ttgcgaatag tataggtgag gaggtaagta tggttcaaga
    16201 aattgcacaa gaaatcattc gttcagctcg gaaaaaaggg acgcaggata tctattttgt
    16261 ccctaagtta gacgcctatg agcttcatat gagggtagga gacgagcgct gtaaaattgg
    16321 tagctatgat tttgaaaagt ttgcagccgt tatcagtcac tttaagtttg tggcgggtat
    16381 gaatgtggga gaaaaaagac gtagtcaact gggttcctgt gattatgcct atgaccataa
    16441 gatagcgtct ctacgtttat ctactgtagg cgattatcgg gggcatgaga gtttggttat
    16501 ccgtttgttg cacgatgagg agcaggacct gcatttttgg tttcaggata ttgaagaatt
    16561 aggcaagcag tacaggcaac ggggactcta tctttttgct ggtccggttg ggagtggtaa
    16621 gacgaccttg atgcatgaat tgtccaagtc actctttaaa ggacagcaag ttatgtccat
    16681 cgaagatcct gtcgaaatca agcaggacga catgcttcag ttgcagttga acgaagcaat
    16741 cggcctaacc tatgaaaatc taatcaaact ttccttgcgt catcgaccag atctcttgat
    16801 tatcggagaa attcgtgaca gcgagacggc gcgtgcagtg gtcagagcta gtttgacagg
    16861 tgcgacagtc ttttcaacca ttcacgccaa gagtatccga ggtgtttatg agcgtctgct
    16921 ggagttgggt gtgagtgaag aagaattggc agttgttctg caaggagtct gctaccagag
    16981 attaatcggg ggaggaggaa tcgttgactt tgcaagcaga gattatcaag aacaccaagc
    17041 agccaagtgg aatgagcaaa ttgaccagct tcttaaagat ggacatatca caagtcttca
    17101 ggctgagacg gaaaaaatta gctacagcta agcaaaaaaa tatcatcacc ctatttaaca
    17161 atctcttttc tagcggtttt catctggtgg agactatctc ctttttagat aggagtgctt
    17221 tgttggacaa gcagtgtgtg acccagatgc gtgtgggctt gtctcagggg aaatcattct
    17281 cagaaatgat ggaaagtttg ggatgttcaa gtgctattgt cactcagtta tccctagctg
    17341 aagttcatgg caatctccac ctgagtttgg gaaagataga agaatatctg gacaatctgg
    17401 ctaaggtcaa gaaaaaattg attgaagtag cgacctatcc cttgattttg ctgggttttc
    17461 ttctcttaat tatgctgggg ctacggaatt acctgctccc acaactggat agtagcaata
    17521 ttgccaccca aattatcggt aatctgcccc aaatttttct aggcatggta gggcttgttt
    17581 ccgtgcttgc ccttttagca ctcacttttt ataaaagaag ttctaagatg agtgtctttt
    17641 ctatcttagc acgccttccc tttattggaa tctttgtgca gacctacttg acagcctatt
    17701 atgcacgtga atgggggaat atgatttcac agggaatgga gttgacgcag atttttcaaa
    17761 tgatgcagga acaaggttcc cagctcttta aagaagtcgg tcaagatctg gctcaaaccc
    17821 tgaaaaatgg ccgtgaattt tctcagacga taggaaccta tcctttcttt aggaaggaat
    17881 tgagtctcat catagagtat ggggaagtta agtccaagct gggtagtgag ttggaaatct
    17941 atgctgaaaa aacttgggaa gcctttttta cccgagtcaa ccgcaccatg aatttggtgc
    18001 agccactggt ttttatcttt gtggcactga ttatcgtttt actttatgcg gcaatgctca
    18061 tgcccatgta tcaaaatatg gaggtaaatt tttaaaatga aaaaaatgat gacattcttg
    18121 aaaaaagcta aggttaaagc ttttacattg gtggagatgt tggtggtctt gctgattatc
    18181 agcgtgcttt tcttgctctt tgtacctaat ctgaccaagc aaaaagaagc agtcaatgac
    18241 aaaggaaaag cagctgttgt taaggtggtg gaaagccagg cagaacttta tagcttagaa
    18301 aagaatgaag atgctagcct aagaaagtta caagcagatg gacgcatcac ggaagaacag
    18361 gctaaagctt ataaagaata caatgataaa aatggaggag caaatcgtaa agtcaatgat
    18421 taaggccttt accatgctgg aaagtctctt ggttttggga cttgtgagta tccttgcctt
    18481 gggcttgtcc ggctctgtcc agtccacttt ttcagcggta gaggaacaga ttttctttat
    18541 ggagtttgaa gaactctatc gggaaaccca aaaacgcagt gtagccagtc agcaaaagac
    18601 tagtctgaac ttagatgggc agacgcttag caatggcagt caaaagttgc cagtccctaa
    18661 aggaattcag gccccatcag gccaaagtat tacatttgac cgagctgggg gcaattcgtc
    18721 cctggctaag gttgaatttc agaccagtaa aggagcgatt cgctatcaat tatatctagg
    18781 aaatggaaaa attaaacgca ttaaggaaac aaaaaattag ggcagtgatt ttactggaag
    18841 cagtagtcgc tctagctatc tttgccagca ttgcgaccct ccttttggga caaattcaaa
    18901 aaaataggca agaggaagca aaaatcttgc aaaaggaaga agtcttgagg gtagctaaga
    18961 tggccctgca gacggggcaa aatcaggtaa gcatcaacgg agttgagatt caggtatttt
    19021 ctagtgaaaa aggattggag gtctaccatg gttcagaaca gttgttggca atcaaagagc
    19081 cataaggtca aggcttttac cttgttagaa tccctgcttg ccctcattgt catcagtggg
    19141 ggattactcc tttttcaagc tatgagtcag ctcctcattt cagaagttcg ctaccagcaa
    19201 caaagcgagc aaaaggagtg gctcttgttt gtggaccaac ttgaggtaga attagaccgt
    19261 tcgcagttcg aaaaagtaga aggcaatcgc ctatacatga agcaagatgg caaggacatc
    19321 gccatcggta agtcaaagtc agatgatttc cgtaaaacga atgctcgtgg tcgaggttat
    19381 cagcctatgg tttatggact caaatctgta cggattacag aggacaatca actggttcgc
    19441 tttcatttcc agttccaaaa aggcttagaa agggagttca tctatcgtgt ggaaaaagaa
    19501 aaaagttaag gcaggtgttc tcctctacgc agtcaccata gcagccatct ttagtctttt
    19561 gttgcaattt tatttgaacc gacaagtcgc ccactatcaa gactatgctt tgaataaaga
    19621 aaaattggtt gcttttgcta tggctaaacg aaccaaagat aaggttgagc aagaaagtgg
    19681 ggaacagttt tttaatctag gtcaggtaag ctatcaaaac aagaaaactg gcttagtgac
    19741 gagggttcgt acggataaga gccaatatga gtttctgttt ccttcagtca aaatcaaaga
    19801 agagaaaaga gataaaaagg aagaggtagc gaccgattca agcgaaaaag tggagaagaa
    19861 aaaatcagaa gagaagcctg aaaagaaaga gaattcatag tcaattcaac tataatgcgt
    19921 tgaatccaga atagtccact gtagtttcta gaaaattgct ggaaatggat gttaagctcc
    19981 aattcatttg tttatatctt atttcagttt actatacttt gtgctaaatt aaagatatga
    20041 aacatgattt taaccacaaa gcagaaactt tcgattcccc taaaaatatc ttcctcgcaa
    20101 acttggtatg tcaagcagcc gagaaacaga ttgatcttct atcagacaaa gaaattttag
    20161 atttcggtgg tggcacgggt ctattagcct tgcccctaac ccctagccaa gcaggctaag
    20221 tcagtcactc ttgtagacat ttctgagaaa atgttggagc aagctcgttt gaaagtggag
    20281 cagcaagcaa tcaagaatat ccagtttttg gagcaagatt taccgaaaaa tcccttggag
    20341 aaagagtttg attgccttgc tgttagtcgg gttcttcatc atatgcctga tttggatgcg
    20401 gctctctcac tgtttcatca acatttgaag gaagatggga aactcatcat tgctgatttt
    20461 accaagacag aagctaatca tcatggattt gatttagctg aactggaaaa caagctaatt
    20521 gagcatggtt tttcatctgt gcatagtcag attctctata gtgctgaaga cctgtttcaa
    20581 ggaaatcact cagaattctt tttaatagta gcccaaaaat cactcgccta gtcagggagt
    20641 gatttttcta taaggatgga aaaaagaagg gaaatttggt aagataggaa tatggatttt
    20701 gaaaaaattg aacaagctta tacctattta ctagagaatg tccaagtcat ccaaagtgat
    20761 ttggcgacca acttttatga cgccttggtg gagcaaaata gcatctatct ggatggtgaa
    20821 actgagctaa accaggtcaa ggagaacaat caaaccctta agcgtttagc actacgcaaa
    20881 gaagaatggc tcaagaccta ccagtttctc ttgatgaagg ctgggcaaac agaacccttg
    20941 caggccaatc accagtttac accggatgct attgctttgc ttttggtgtt tattgtggaa
    21001 gagttgttta aagaggagga aattactatc ctcgaaatgg gttctgggat gggaattcta
    21061 ggcgctattt tcttgacctc gcttactaaa aaggtggatt acttgggaat ggaagtggat
    21121 gatttgctga ttgatctggc agctagcatg gcagatgtaa ttggtttgca ggctggcttt
    21181 gtccaaggag atgccgttcg cccacaaatg ctcaaagaaa gcgatgtggt catcagtgac
    21241 ttgcctgtcg gctattatcc tgatgatgcc gttgcgtcgc gccatcaagt tgcttctagc
    21301 caagaacata cttacgccca tcacttgctc atggaacaag ggcttaagta cctcaagtca
    21361 gacggatacg ctatttttct agctccgagt gatttgttga ccagtcctca aagtgatttg
    21421 ttaaaagaat ggctgaaaga agaggcgagt ctggttgcta tgattagtct gcctgaaaat
    21481 ctctttgcta atgccaaaca atctaagact atttttatct tacagaagaa aaatgaaata
    21541 gcagtagagc cttttgttta tccacttgct agcttgcaag atgcaagtgt tttaatgaaa
    21601 tttaaagaaa attttcaaaa atggactcaa ggtactgaaa tataaaatag attttgttat
    21661 aatagttgaa aacgcttaaa aaggggtatc atgttatgac aaaaacaatt gcaatcaatg
    21721 caggaagttc aagtttgaaa tggcaattat acttaatgcc agaagaaaaa gtattggcga
    21781 aaggtttgat tgaacgtatc ggtttgaaag attcaatttc aactgtaaaa tttgacggcc
    21841 gttctgaaca acaaattttg gatattgaaa atcatataca agccgttaaa attttattgg
    21901 atgacttgat tcgtttcgat attatcaagg cttatgacga gattacaggt gttggacatc
    21961 gtgttgttgc tggtggagaa tatttcaaag aatcaacagt tgttgaggga gatgttttag
    22021 aaaaagttga agagttgagt ttgttggctc ctctacacaa cccggccaat gcagcaggtg
    22081 ttcgtgcctt caaggaattg ttgccagaca ttaccagtgt agttgttttt gatacttcct
    22141 tccacacaag tatgccagag aaagcttatc gctaccctct accaacaaaa tattacacag
    22201 aaaacaaggt tcgtaaatac ggtgctcatg gtacaagtca ccagtttgta gcaggagaag
    22261 ctgcaaaact cttgggacgt ccattagaag acttgaagtt aattacctgt catattggta
    22321 acggaggctc aattacagct gtgaaagccg gcaaatctgt agacacttct atggggttca
    22381 ctcctcttgg tggtattatg atgggaacgc gtacagggga tattgatcca gctatcattc
    22441 cttatttaat gcaatataca gaggatttta acacaccaga agatatcagt cgtgttctta
    22501 accgtgaatc aggtcttttg ggagtttctg ctaattctag cgatatgcgc gatatagaag
    22561 cagctgtagc agaagggaat cacgaggcta gcttggctta tgaaatgtat gttgaccgta
    22621 tccaaaaaca tatcggtcag taccttgcag tgctaaatgg agcagatgcc attgttttca
    22681 cagcaggtgt cggtgaaaat gcagagagtt tccgtcgtga tgtaatctca gggatttcgt
    22741 ggtttggttg tgatgttgat gatgaaaaga atgtctttgg cgttacagga gacatctcaa
    22801 cagaggcagc taaaatccgt gtcttggtta ttccaacaga tgaagaatta gtcattgccc
    22861 gtgacgttga acgcttgaaa aaataagtga aactaaaaaa atattcaata caaggagttg
    22921 ggaaagttat ttttccagct tctttttctg atgaaattgt ccaaaacctt gctatgattg
    22981 gcttttttga aaaatatggt ataatagtag taatttaata gatggagttg agttttgaag
    23041 aaaaactttc gtgtaaaaag agagaaagat tttaaggcga ttttcaagga ggggacaagt
    23101 tttgctaatc gcaaatttgt ggtctaccaa ttagaaaacc agaaaaaccg ttttcgagta
    23161 ggtctatcag ttagcaaaaa actggggaat gccgtcacta gaaatcaaat taagcgacgg
    23221 attcggcata ttatccagaa tgcaaaaggg agtctggtag aagatgtcga ctttgttgtc
    23281 attgctcgaa aaggagtcga aaccttggga tacgcagaga tggagaaaaa tctactccat
    23341 gtattaaaat tatcaaagat ttaccgggaa ggaaatggga gtgaaaaaga aactaaagtt
    23401 gactagtttg ctaggactgt ctctgttaat catgacagcc tgtgcgacta atggggtaac
    23461 tagcgatatt acagccgaat cggctgattt ttggagtaaa ttggtttact tctttgcgga
    23521 aatcattcgc tttttatcgt ttgatattag tatcggagtg gggattattc tctttacggt
    23581 cttgattcgt acagtcctct tgccagtctt tcaggtgcaa atggtggctt ctaggaaaat
    23641 gcaggaagct cagccacgca ttaaggcgct tcgagaacaa tatccaggtc gagatatgga
    23701 aagcagaacc aaactagagc aggaaatgcg taaagtattt aaagaaatgg gtgtcagaca
    23761 gtcagactct ctttggccga ttttgattca gatgccggtt attttggccc tgttccaagc
    23821 cctatcaaga gttgactttt taaagacagg tcatttctta tggattaacc ttggtagtgt
    23881 ggatacaacc cttgttcttc cgattttagc agcagtattc acctttttaa gtacttggtt
    23941 gtccaacaaa gctttgtctg agcgaaatgg cgctacgact gcgatgatgt atgggattcc
    24001 agtcttgatt tttatctttg cagtttatgc gccaggtgga gtcgccctat actggacagt
    24061 gtctaatgct tatcaagtct tgcaaaccta tttcttgaat aatccattca agattatcgc
    24121 agagcgcgag gccgtagtac aggcacaaaa agatttggaa aatagaaaaa gaaaagccaa
    24181 gaaaaaggct cagaaaacga aataaataag gaggaatctg gtagtggtag tatttacagg
    24241 ttcaactgtt gaagaagcaa tccagaaagg attgaaagaa ttagatattc caagaatgaa
    24301 ggctcatatc aaagtcattt ctagggagaa aaaaggcttt cttggtctat ttggtaaaaa
    24361 accagcccaa gtggatattg aagcgattag tgaaacgacg gttgtcaaag caaatcaaca
    24421 ggtagtaaaa ggcgttccga aaaaaatcaa tgatttgaac gagcctgtga agacggttag
    24481 tgaagaaacc gttgaccttg gtcatgtggt tgatgctatt aaaaaaatag aggaagaagg
    24541 tcaaggtatt tctgatgaag tcaaggctga aatcttaaaa catgaaagac atgccagcac
    24601 tatcttagaa gaaactggtc acattgagat tttaaatgaa cttcaaatcg aggaagcgat
    24661 gagggaagaa gcaggcgctg atgaccttga aactgagcaa gaccaagctg aaagtcaaga
    24721 actagaagac ttgggcttga aagttgaaac gaactttgat attgaacaag tagctacgga
    24781 agtaatggct tatgttcaaa cgattattga tgacatggat gttgaggcta cactttcaaa
    24841 tgattataac cgtcgtagca tcaatctaca aattgacacc aacgaaccag gtcgtattat
    24901 cggctaccat ggtaaagtct tgaaggcctt gcaactgttg gctcaaaatt atctttacaa
    24961 ccgctattcc agaaccttct acgttacaat caatgtcaat gattatgtcg aacaccgtgc
    25021 agaagtcttg cagacctatg cgcaaaaatt ggcgactcgt gttttggaag aagggcgcag
    25081 tcataaaaca gatccaatgt caaatagcga acgcaagatt atccatcgta ttatttcacg
    25141 tatggatggc gtgactagtt actctgaagg tgatgagcca aatcgctatg ttgttgtaga
    25201 tacagaataa gtaaaatcag gtttatcctg attttttgct agttagagga ggttaaactg
    25261 atgttgaata agataagaga ctatttagac tttgctggtt tgcagtaccg taatcctgat
    25321 aaagcgggag cagagcgaga gaagatgctg gcattccgcc acaaaggaca agaggcccga
    25381 aaggttttta cagaactggc caaagccttt caagcaagcc atccagaatg gcaactccaa
    25441 cagactagcc agtggatgaa tcaggcccag cgtttgagac cacatttttg ggtttatcta
    25501 cagagagacg gacaagtgac agaacctatg atggccttac gtttgtatgg gacatctact
    25561 gactttggaa tttctttgga agtcagtttc atcgaacgta agaaggatga gcaaacactg
    25621 ggcaagcagg ccaaagtttt agacattcca accgttaaag ggatttatta tctaacctac
    25681 tctaatggtc aaagtcaacg gtgggaggcg aatgaagaaa agcgtcgtac tttacgcgag
    25741 aaggtgagaa gtcaagaagt tcgaaaagtt ttagtgaagg tagatgttcc tatgacagaa
    25801 aattcgtctg aagaagaaat cgtagaaggc ttattgaagt cttattctaa aattcttccc
    25861 tattatctag ctacgagaaa ataagataat ttgtaaaaca tcataaatca tacagtccaa
    25921 gagtgaacag tccgctgtgt aattcttggt ctttttgttt gcgctttcgc attatataat
    25981 aaacttacaa aaacaattca aaaggagaac aattatggaa gtcgtttcaa gtgttctaaa
    26041 ttggttttct agcaatattt tgcagaatcc cgcatttttc gtaggtttat tggtgttgat
    26101 aggatatgca cttttgaaaa aacctgccca tgacgttttt tcagggtttg ttaaagcaac
    26161 agtagggtat atgttgctta acgtgggtgc tggtggtttg gttacaacct ttcgtccaat
    26221 cttagcagct cttaactaca aattccaaat tggtgcagcg gttatcgacc cttactttgg
    26281 acttgctgca gcaaacaaca aaattgtagc agagtttcca gattttgttg gaactgcaac
    26341 tacagctcta ttgattggtt ttggaataaa tatcttgctc gtagctcttc gaaagattac
    26401 gaaggtaaga accctcttta ttactggtca catcatggta caacaagctg caacagtatc
    26461 tcttatggtt ctattcttag taccacaatt gcgcaatgct tacggtacag cagcgattgg
    26521 tatcatctgt ggactttact gggcagttag ttcaaatatg actgttgagg caactcaacg
    26581 cttgactggt ggtggcggat ttgcgattgg tcaccaacag caatttgcaa tctggtttgt
    26641 agataaagta gcaggacgct ttggtaagaa agaagaaagt ttagacaatc ttaaattacc
    26701 taagttcctc tcaatcttcc acgatacagt tgttgcatct gctaccttga tgctcgtatt
    26761 cttcggagcc attcttttaa tcttgggtcc agacattatg tctaataaag aagtcatcac
    26821 ttcaggaact ctattcaatc ctgctaaaca agatttcttt atgtacatta tccaaacagc
    26881 ctttaccttc tcagtttact tgttcgtttt gatgcaaggt gtccgaatgt tcgtatctga
    26941 gttgacaaac gccttccaag gtatttcaaa caaattgttg ccaggttcat tcccagcggt
    27001 tgacgttgca gcttcttatg gatttggttc tccaaatgct gtcttgtcag gatttacctt
    27061 tggtttgatt ggtcaattga ttacaattgt tttgctcatc gtctttaaaa atccgattct
    27121 tattattaca ggatttgtac cagtgttctt tgacaatgca gccattgcgg tctacgctga
    27181 taaacgcggc ggatggaaag cggctgttat cctttccttt atatcaggtg tccttcaagt
    27241 tgctctagga gctctttgtg tggcccttct cgatttggca tcttatggtg gctaccatgg
    27301 aaatatcgac tttgaattcc catggcttgg atttggatat atcttcaaat accttggtat
    27361 tgttggttat gtacttgtgt gtctcttctt gcttgttatt cctcaacttc aatttgccaa
    27421 agcaaaagat aaagagaaat attacaacgg tgaagttcaa gaagaagctt agtatctaga
    27481 aaaggagaaa taaaatggtt aaagtattag cagcgtgcgg aaatggaatg ggttcatcaa
    27541 tggttatcaa gatgaaggtt gaaaatgctc tccgtaagct taatcaaaca gattttacag
    27601 tcaattcatg cagtgtcggt gaagctaaag gtttagcagt aggatatgac atcgtaatcg
    27661 cttctcttca tttgattcaa gaattggaag ggcgaactaa tgggaagtta attgggcttg
    27721 ataacttgat ggatgataaa gaaatcaccg aaaaactcag tcaagcacta cagtaaaagg
    27781 ttggaggggc tggacagaaa ctgagagtta tcgtttctgt ccttctccct ctttaaataa
    27841 aggaggcaga tatgaattta aaacaagctt taattgacaa tgactcgatc cgactaggtt
    27901 tagaggctaa caattggaaa gaagcagtca aggtagcagt agatccctta attgaaagtg
    27961 gggcaatttt gccagagtat tacgatgcta tcattgaatc gactgaagag tatgggcctt
    28021 actatatctt gatgccaggt atggctatgc cccacgctag acctgaagct ggtgtgcaaa
    28081 gtgatgcctt ttcattgatt accttacaaa atcctgttgt attttcagat gggaaagagg
    28141 tatctgtttt gttggcacta gcagcaacaa gttcaaaaat tcacacaagt gtagccattc
    28201 cacaaattat tgccctgttt gaattagaag attctattgc acgtttacag gcttgccaga
    28261 ctaaagaaga tgtcttggct atgattgaag aatctaagga tagcccttat ctcgaaggat
    28321 tggatttgga aagttagaaa gaggaataaa gaaatgacaa aaagaatacc taatttacaa
    28381 gttgcattag accattcaga cttgcaagga gcgattaaag cagctgtttc tgttggtcag
    28441 gaagtagata ttatcgaagc tggaactgtt tgcttgcttc aagttggaag tgaactggct
    28501 gaagtcttgc gtagcctttt cccagataag attattgtgg cagacacaaa atgtgctgat
    28561 gctggtggaa cagttgctaa aaataatgcg gttcgtggag cagactggat gacttgtatc
    28621 tgttgtgcaa ccatccctac tatggaagca gctctaaagg ctatcaagac tgaacgagga
    28681 gaacgaggcg aaatccagat cgagctttat ggcgattgga cttttgaaca agctcagctt
    28741 tggctagatg caggtatctc acaagctatt tatcaccaat ctcgtgatgc tcttcttgct
    28801 ggtgaaactt ggggtgaaaa agaccttaat aaggttaaaa aactcattga catgggcttc
    28861 cgtgtatctg taacaggtgg tctagatgta gatactctca aactctttga aggtattgat
    28921 gtctttacct ttatcgcagg tcgtggaatt acagaggctg tggatccagc aggagcagcg
    28981 cgtgccttca aggatgaaat caaacgaatt tgggggtaaa tcatggtacg tccaattgga
    29041 atttatgaaa aggcaacccc aacacactgt acttggctag aacgtttaaa ttttgccaag
    29101 gagttaggct ttgattttgt cgagatgtct attgacgaac gtgacgagcg tttagcaaga
    29161 cttgactgga gtaaggaaga acgcttggaa gttgtcaaag caatctatga aactggtgtt
    29221 cgtattcctt ctatctgttt ttcaggccat cgtcgctacc cattgggttc aaaagatcca
    29281 gttctagagg aaaaatctct agaactcatg aaaaaatgta tcgaattagc tcaagacttg
    29341 ggagttcgta cgattcaatt agctggttac gatgtttact atgaggaaaa gtcaccccag
    29401 acacgccaac gttttatcaa aaatttgaga aaagcctgtg actgggctga agaagctcag
    29461 gtggtacttg ctattgaaat tatggatgat cctttcatca gtagcatcga aaaatatttg
    29521 gctatagaaa aagagattga ctctcccttc ctctttgtat atccagatat tggtaatgtg
    29581 tctgcatggc ataatgatat ctatagtgag ttttatcttg gtcatcatgc catcgcagct
    29641 ctccatctca aggatactta tgcagtgaca gaaagttcaa agggccagtt ccgagatgta
    29701 cctttcgggc aaggttgtgt caaatgggaa gaagctttcg atattttaaa ggaaaccaat
    29761 tataatggac ctttcctaat cgaaatgtgg tctgaaaatt gtgaaacagt agaagaaaca
    29821 cgcgcagcca ttcaagaggc gcaagctttt ctctatccac tcattaagaa agcaggtttg
    29881 atgtaagatg aatcaagtaa tcaatgctat gcgtaaacga gtttgtgatg ccaatcaatc
    29941 attgccaaaa catggacttg tcaaatttac ctgggggaat gtatctgaag tcaatcgcga
    30001 actcggtgtc attgttatca aaccatcagg cgtggattat gacgaattga cacctgaaaa
    30061 catggtagtg actgatctag atggtaagat cctagaaggg gatttaagac catcttccga
    30121 cctcccaact catgtgcaat tatataaggc ttggtcagaa attggtagtg tggttcacac
    30181 ccattcgaca gaagctgttg gttgggctca ggcaggtcgt gatattcctt tctacggaac
    30241 aacccatgca gattatttct acggttcaat cccttgcgcc cgtagtttga ccaaggacga
    30301 agtagaagtg gcctatgaaa aagatactgg cctggttatc gtagaagaat ttgaacatcg
    30361 cggacttaac ccggttgaag taccaggaat tgttgtacgc aatcacggtc cattcacctg
    30421 gggcaaaaat ccagagaatg ctgtttatca ctctgtcgta ctagaggaag tatcaaagat
    30481 gaatcgcttt acagaacaaa tcaatccaag agttggacct gctccccagt acatactaga
    30541 aaaacactac caacgtaaac atggaccaaa tgcttattat ggtcaaaagt aagaacgatg
    30601 aaggaggaga aaaagataaa tttagctcct ctttttacat ttgattttta ttgagagtaa
    30661 agttggagtt gaagtaattt tgaaagattt tttagaaata gcgcttgata tatatggtaa
    30721 aataaaaaga attgctgtga tatcaataga tttgggggat tttttaatat ggtactggat
    30781 aaggcaagtt gtgatttgct tcaatatttg atggatcaag aaacgtccaa aacgattatg
    30841 gcgatttcga aagatttgaa agagtcaaga aggaaaattt attatcacat tgacaaaatc
    30901 aatgctgctc tggttgacga ggcgcttcac atcattagta ttccacgaat tggtattcac
    30961 ttaacggaag agcagagaga tgcttgttgt aaactattat cggaagtaga ttcgtacgat
    31021 tatatcatga gtgcgcatga acgtatgatg ataatgttac tatggatagg tatttctaaa
    31081 gaacgtatta cgattgaaaa attgatagag ttaacagagg tatctaggaa tactgttctc
    31141 aatgatttga atagtattcg ttatcaacta actttggaac aatatcaggt gatcttgcaa
    31201 gtgagcaagt cacagggata ccaccttcat gcccaccctc ttaataaaat tcagtatctt
    31261 caatcgcttc tatatcatat ttttatggaa gaaaatgcca cttttgtatc tattttagaa
    31321 gataagatga aagagaggtt agatgatgag tgtttgcttt ctgttgaaat gaaccaattt
    31381 tttaaggaac aggttccttt agttgaacaa gatttaggga agaaaataaa ccatcatgaa
    31441 ataactttta tgttgcaggt tctaccttat ttgctgttaa gctgtcataa tgttgaacag
    31501 tatcaagaaa gacatcagga tatagagaaa gaattttctt tgataagaaa aagaatagag
    31561 tatcaggtgt ctaagaaatt aggagaacgg ttgtttcaaa agtttgaaat ttctttgtca
    31621 ggacttgaag tttctcttgt agctgttctc ctcctctcct atcgtaagga tttggatatt
    31681 catgcagaaa gtgatgattt tcggcaatta aaacttgctt tagaagaatt tatctggtat
    31741 tttgaatcac aaatccgaat ggagattgag aacaaggatg atttgttacg aaatttgatg
    31801 atccactgta aagccttgtt atttagaaag acttacggta ttttttctaa aaatcctcta
    31861 acaaaacaaa ttcgatccaa gtatggagaa ttatttttag tcactagaaa atctgcggaa
    31921 attttagaag gagcatggtt tattcggcta acagacgatg atattgccta tttgacgatt
    31981 catattggag gatttttaaa gtatacacca tcgtctcaaa aaaatatgaa aaaagtttat
    32041 ctcgtttgtg atgaaggtgt tgcggtttcg agacttttgc tgaaacaatg caaactttat
    32101 tttccaaatg agcaaattga cactgtattt acaacagaac aatttaagag tgtggaagat
    32161 attgcacaag ttgatgtagt gattactact aatgatgatt tggatagcag atttccgatt
    32221 ttaagggtta atcctatcct tgaagcagaa gatattttga aaatgctaga ctatcttaaa
    32281 cacaatatat ttcgtaataa gagcaaaagt ttcagtgaaa atctttctag tcttatttcg
    32341 tcttatattg tagacagcaa gttggctagt aagttccaag aagaggttca aacacttata
    32401 aatcaagaaa tagtagttca agcttttttg gaagatattt gaaggacagt ccaatgatga
    32461 acacaaacct gtgtttttct tggtcttttt tagtgttttg aagggtggta tactaatctc
    32521 aaagataaca attatatcca aaggaggcaa catatgccaa acgtcaaaga aattacaaga
    32581 gagtcatgga ttttagccac tttcccagag tggggaacat ggttgaacga agaaatcgaa
    32641 gaagaagtcg tacctgaagg caattttgcc atgtggtggc taggcaactg tggtacttgg
    32701 attaagacac cagctggtgc taacgttgtc atggaccttt ggtcaaaccg tggaaaatca
    32761 accaaaaaag tgaaagatat ggttcgtggg caccaaatgg caaatatggc aggtgttcgt
    32821 aagttgcaac caaacttgcg tgttcagcca atggttatcg atccatttgc tatcaacgaa
    32881 ctagactatt acttagtttc acacttccac agtgatcata tcgacccata cacagctgca
    32941 gcaattctca acaattctaa gttagagcat gttaagttta tcggtcctta ccactgtgga
    33001 cgaatctggg aaggatgggg tgttccaaaa gaacgtatca tcgttgttaa accaggtgac
    33061 actatcgaat taaaagatat gaagattcat gcagtagaat catttgaccg tacttgcttg
    33121 gtaactctcc cagtgaacgg tgctgatgag acaggcggtg aacttgctgg cttggctgtt
    33181 acagatgaag aaatggctca aaaggctgtt aactatatct ttgaaacacc aggtggaacc
    33241 atctatcatg gtgcagattc tcacttctca aactattttg caaaacatgg taaagacttt
    33301 aaaattgatg ttgctttgaa taactatggt gaaaatccgg taggtatcca agacaaaatg
    33361 acatctatcg atcttcttcg tatggcagaa aatctgcgta ccaaagtcat tatcccagtt
    33421 cactatgata tctggtctaa cttcatggct tctactaatg agattctaga actttggaaa
    33481 atgcgaaaag atcgcttgca atacgatttc catccattta tctgggaagt tggcggtaag
    33541 tacacttatc ctcaagatca acacttagta gaataccatc atccacgtgg ttttgatgat
    33601 tgttttgaac aagactctaa cattcaattt aaagctttgc tataatataa aaatattctt
    33661 tggaggttat ccgtatgttg catgatacgg ataattttca tataatagta aaatagaatg
    33721 tgtgattcaa taatcacctc aaatagaaag gaaattctat gtcaaatcta tctgttaatg
    33781 caattcgttt tctaggtatt gacgccatta ataaagccaa ctcaggtcat ccaggtgtgg
    33841 ttatgggagc ggctccgatg gcttacagcc tctttacaaa acaacttcat atcaatccag
    33901 ctcaaccaaa ttggattaac cgcgaccgct ttattctttc agcaggtcat ggttcaatgc
    33961 tcctttatgc tcttcttcac ctttctggtt ttgaagatgt cagcatggat gagattaaga
    34021 gtttccgtca atggggttca aaaacaccag gtcacccaga atttggtcat acggcaggga
    34081 ttgatgctac gacaggtcct ctagggcaag ggatttcaac tgctactggt tttgcccaag
    34141 cagaacgttt cttggcagcc aaatataacc gtgaaggcta caatatcttt gaccactata
    34201 cttacgttat ctgtggagac ggagacttga tggaaggtgt ctcaagcgag gcagcttcat
    34261 acgcaggttt gcaaaaactt gataagttgg ttgttcttta tgattcaaat gatatcaact
    34321 tggatggtga gacaaaggat tcctttacag aaagtgttcg tgaccgttac aatgcctacg
    34381 gttggcatac tgccttggtt gaaaatggaa cagacttgga agccatccat gctgctatcg
    34441 aaacagcaaa agcttcaggc aagccatctt taattgaagt gaagacggtt attggatatg
    34501 gttctccaaa caaacaagga actaatgctg tacacggcgc ccctcttgga gcagatgaaa
    34561 ctgcatcaac tcgccaagcc ctcggttggg actacgaacc atttgaaatt ccagaacaag
    34621 tatatgctga tttcaaagaa catgttgcag accgtggcgc atcagcttat caagcttgga
    34681 ctaaattagt tgcagattat aaagaagctc atccagaact ggctgcagaa gtagaagcca
    34741 tcatcgacgg acgtgatcca gtcgaagtga ctccagcaga cttcccagct ttagaaaatg
    34801 gtttttctca agcaactcgt aattcaagtc aagatgcctt gaatgttgtg gcagctaaat
    34861 taccaacctt cctaggtgga tcagctgacc ttgctcactc aaacatgact tatatcaaga
    34921 cggatggact tcaagacgat accaatcgct tgaatcgtaa cattcagttt ggtgttcgtg
    34981 aatttgcaat gggaacaatc ttgaacggga tggcccttca tggtggactt cgtgtatacg
    35041 gtggaacttt cttcgtcttc tctgactatg tgaaggcagc tgtccgcttg tcagccttac
    35101 aaggacttcc tgtgacttat gtctttaccc atgattcaat cgcagttggg gaagatggtc
    35161 cgactcacga accagttgag catttagcag gtcttcgtgc tatgccaaat ctaaatgttt
    35221 tccgtccagc agatgcgcgt gaaacgcaag cagcttggta ccttgcagtg acaagtgaga
    35281 aaacaccaac tgcccttgtc ttgacacgtc aaaacttgac tgttgaagat ggaacagact
    35341 tcgacaaggt tgctaaaggt gcttatgttg tatatgaaaa tgcagccgac tttgatacca
    35401 tcttgattgc gacaggttca gaggttaatc ttgctgtctc agctgctaaa gaattggcta
    35461 gtcaaggcga aaaaatccgc gtaatcagca tgccatctac agatgtcttt gataaacaaa
    35521 atgcagctta caaggaagag attcttccaa atgcagtccg ccgtcgtgtt gcagtcgaaa
    35581 tgggtgcaag tcaaaactgg tacaaatatg ttggtctcga tggtgccgtt ctaggtattg
    35641 ataccttcgg agcctctgcc ccagcaccaa aagtattggc agaatatggc tttactgtcg
    35701 aaaatcttgt aaaagttgtt cgaaatttga aataatccta aaaatcaggg cgtaagctct
    35761 ggtttttctt accagaaaag taaggtacaa tcttgtaaaa gtagctgaaa tttgatatag
    35821 tagtcctatg taaaagacaa aggagaaaac aatgaatcca aatattactt ttttaatcat
    35881 gcttgtaggt atgatggcct tgatgttctt tatgcaacgt tctcaaaaga aacaagctca
    35941 aaagcgtatg gaaagcttaa ataaactaca aaaaggctat gaagtgatta caatcggtgg
    36001 actttatgga acagtcgatg aagtagatac ggagaaggga acaatagttc ttgacgtaga
    36061 tggagtttac ttgacttttg aactagctgc tatcaagaca gtattaccgc tgaaagaaac
    36121 agcttcacta gaaggcgcaa ttgaaaaata agacgggatc acgaactccc gttttctata
    36181 aaagaaagga aatgggatga aaaaattagt ctttgtctgt ctgggaaata tttgccgtag
    36241 ccctatggcc gagtttgtta tgaaatcaat gacagataac tacgaaatcc aaagtcgagc
    36301 aacttcctct tgggaacatg gcaatccgat tcataagggg actcagggaa tttttcaaga
    36361 gtatgagatt ccttatgaca agaacaagac atcgcttcag attagtaagg aagattttga
    36421 agcctttgat tatattatcg gaatggacgc ttcaaatatt tctgacttac gtcagatgtg
    36481 tccagtagac tgtcaagata agatttactc attttcatct gaaagtgttc cagacccttg
    36541 gtatacagga gattttgaag aaacctatcg acgtgttcaa gagggctgtc aagcttggtt
    36601 agaacgttta gaaaaggaga gttaaggtgg aaaatcttac gaatttttac gaaaagtatc
    36661 gtgtctatct gactcgtcca cgtttagagc ttttggcagt agttaccatt gttttctgtg
    36721 ctgtactcgt cttttttcta aatattccag gaaaaggtgt cttaaaactc gataatggaa
    36781 cgattgttta tgatggcagt cttgtccgtg gtaaaatgaa tggccaaggt accattacct
    36841 tccaaaatgg agaccaatat acaggtggct tcaacaatgg agccttcaac ggaaaaggta
    36901 cctttcaatc taaagaaggc tggacctacg aaggtgattt tgtaaatggt caggctgaag
    36961 gaaaagggaa actaacaaca gaacaagaag tcgtttatga aggaactttt aaacaaggcg
    37021 tttttcaaca aaaataaagc ctccttatca aaggaggtat tattagaatc acaaggtaag
    37081 cgcttacctg taaatccctt tctttccaaa tccctcttcc aagcaagttt gtgaaataaa
    37141 aaatatttga aataaatttc acaaacttca aagataaaac ttgataagaa aagaaaatga
    37201 gaaaagtttc acaagagttt aaaaattctt tgtgaaatgt ttatgaaact gtttacaaag
    37261 ttgaaataat gcgttataat aaacttgtga aaaaattaac aaaggatatc gttccttgaa
    37321 agctatggag gaaaatatgg ctgataaaaa aactgtgaca ccagaggaaa agaaactcgt
    37381 tgctgaaaaa cacgtagatg agttggttca aaaagctcta gttgcccttg aagaaatgcg
    37441 taaattggat caagaacaag ttgactacat cgttgccaaa gcatcagtag cagctttgga
    37501 tgcccacgga gaattggctt tacatgcctt tgaagaaaca ggacgtggtg tatttgaaga
    37561 caaagcaact aagaacttgt ttgcttgtga acacgtagta aacaatatgc gccacactaa
    37621 gacagttggc gttatcgaag aagacgatgt aacaggattg actcttattg ctgaaccagt
    37681 tggtgttgtt tgtggtatta ctccaacaac aaacccaaca tcaacagcaa tcttcaaatc
    37741 attgatttca ttgaagacac gtaacccaat cgtctttgcc ttccatccat cagcacaaga
    37801 atcatctgct catgcagctc gtatcgtccg cgatgcagct atcgcagctg gtgctcctga
    37861 aaactgtgtg caatggatta ctcaaccatc tatggaagca acaagtgccc ttatgaacca
    37921 cgaaggtgtt gcgacaatcc ttgcaacagg tggtaatgcc atggttaagg cggcttattc
    37981 atgtggtaaa ccagctcttg gggtaggtgc cggaaacgtt ccagcttatg ttgaaaaatc
    38041 agcaaacatt cgtcaagcag cacacgatat cgtcatgtct aaatcatttg ataacggtat
    38101 ggtctgtgca tctgaacaag cagttatcat tgataaagaa atttacgatg aatttgtagc
    38161 agagttcaaa tcttaccaca cttactttgt aaacaaaaaa gaaaaagctc ttcttgaaga
    38221 gttctgcttc ggcgtcaaag caaacagcaa aaactgtgct ggtgcaaaat tgaacgctga
    38281 catcgttggt aaaccagcaa cttggattgc agaacaagca ggatttacag ttccagaagg
    38341 aacaaacatt cttgctgcag aatgtaaaga agttggcgaa aatgagccat tgactcgtga
    38401 aaaattgtca ccagttattg cagttttgaa atctgaaagc cgtgaagatg gtattactaa
    38461 ggctcgtcaa atggttgaat ttaacggtct tggacactca gcagctatcc acacagctga
    38521 cgaagaattg actaaagaat ttggtaaagc tgttaaagct attcgtgtta tctgtaactc
    38581 accttctact tttggtggta tcggggacgt ttacaatgcc ttcttgccat cattgacact
    38641 tggatgtggt tcttacggac gcaactcagt tggggataac gttagtgcca ttaacctctt
    38701 gaatatcaaa aaagtcggaa gacggagaaa taacatgcaa tggatgaaac ttccttcaaa
    38761 aacatacttt gaacgtgatt caattcaata ccttcaaaaa tgtcgtgacg ttgaacgtgt
    38821 catgatcgtt actgaccatg ccatggtaga gcttggtttc cttgatcgta tcatcgaaca
    38881 actggacctt cgtcgcaata aggttgttta ccaaatcttt gcggatgtag aaccggatcc
    38941 agatatcaca actgtaaacc gtggtactga gattatgcgt gccttcaaac cagataccat
    39001 catcgcactc ggtggtgggt ctccaatgga tgctgccaaa gtaatgtggc tcttctacga
    39061 gcaaccagaa gtggacttcc gtgaccttgt ccaaaaattc atggatatcc gtaaacgtgc
    39121 cttcaagttc ccattgcttg gtaagaagac taaattcatc gcgattccaa ctacatctgg
    39181 tacaggatct gaagtaacac catttgccgt tatctctgat aaagcaaaca accgtaaata
    39241 cccaatcgct gactactcat tgacaccaac tgtggcaatc gtagaccctg ctttggtatt
    39301 gacagttcca ggatttgttg ctgctgatac tggtatggac gtattgactc acgcgacaga
    39361 agcatacgta tcacaaatgg ctagtgacta cactgatggt ttagcacttc aagccattaa
    39421 attggtcttt gaaaatctcg aaagctcagt taagaatgca gacttccact cacgtgagaa
    39481 aatgcataac gcttcaacaa tcgctggtat ggcctttgcc aatgccttcc taggtatttc
    39541 tcactcaatg gcccataaga ttggtgcgca attccacaca atccacggtc gtacaaatgc
    39601 tatcttgctt ccatacgtta tccgttacaa cggtacacgt ccagctaaga cagcaacatg
    39661 gcctaagtac aactactacc gtgcagatga aaaataccaa gatatcgcac gcatgcttgg
    39721 acttccagct tctactccag aagaaggggt tgaatcttac gcaaaagctg tctacgaact
    39781 cggtgaacgt attgggatcc aaatgaattt tagagaccaa ggaattgacg aaaaagaatg
    39841 gaaagaacat tctcgtgaat tatccttcct ggcttatgaa gaccaatgtt caccagctaa
    39901 cccacgtctt ccaatggtag accatatgca agaaatcatc gaagatgcat actatggcta
    39961 caaagaaaga ccaggacgcc gtaaataatt gtttatcagt ctagaagcaa gacaaaaact
    40021 caatttgagg gaaagatcca gtaatttttc tatgataaaa ggcatcctat caaggttttt
    40081 gaatacctga taggatgcct ttttatgatg ttgaggcctt tttgcccttt ttgaaaaact
    40141 agaatagaaa caaaatatat agcagattga aactagaata gtacatatct gcttctaaaa
    40201 cattgttaga attcgatttg actgtcctga tcga
//
LOCUS       DAVHEX010000010        39382 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVHEX010000010 DAVHEX010000000
VERSION     DAVHEX010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 39382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39382
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.010"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(566..1702)
                     /locus_tag="VVL45_000543"
     CDS             complement(566..1702)
                     /locus_tag="VVL45_000543"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEU4186762.1"
                     /translation="MKHSVHFGAGNIGRGFIGEILFKNGFHIDFVDVNNQIIHALNEK
                     GKYEIEIAQKGQSRIEVTNVAGINSKEHPEQVIEAIQRTDIITTAIGPNILPFIAELL
                     AKGIEARRVAGNTQALDVMACENMIGGSQFLYQEVKKYLSPEGLTFADNYIGFPNAAV
                     DRIVPAQSHEDSLFVVVEPFNEWVVETKRLKNPDLRLEDVHYEEDLEPFIERKLFSVN
                     SGHATSAYIGAHYGAKTILEALQNPNIKSRIESVLAEIRSLLIAKWNFDKKELENYHK
                     VIIERFENPFIVDEVSRVARTPIRKLGYNERFIRPIRELKELSLSYKNLLKTVGYAFD
                     YRDVNDEESIRLGELLAKQSVKDVVIQVTGLDDQELIEQIVEYI"
     gene            complement(1764..2201)
                     /locus_tag="VVL45_000544"
     CDS             complement(1764..2201)
                     /locus_tag="VVL45_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU4186763.1"
                     /translation="MKLEKHLIKLNKQFSNKEEAICYCGQVLYEGGYVNEDYIEAMIE
                     RDKELSVYMGNFIAIPHGTDAAKKDVLKSGITVVQVPRGVDFGNVSNPQVATVLFGIA
                     GIGNEHLEIIQKISIFCADVDNVLKLADAQSKEEVLRLFDAVE"
     gene            complement(2203..4158)
                     /locus_tag="VVL45_000545"
     CDS             complement(2203..4158)
                     /locus_tag="VVL45_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU4186764.1"
                     /translation="MLLTKREEQLLKAFLHVGKLSMQDMTEILQVSSRTIYRTLSDLT
                     DSMEQYGIEITKHGKYYILTGELDDLPTELEVLVEYSPQERQELITYRLLTESGFVTN
                     EALQECTKVSNVTIIQDISDIDKRLLDFDLKIERQKGYRISGDSVGKRRFLAILLTNC
                     ISVADFSTGNFGSFDILEADRTRLASQIVNKQLSGFPDMDARMKMFFAILLSLIGQEQ
                     NIENSPNTSKQALEISQKIFQTYSKQTAQFYSIQEIIYFASILDELIIKRQDNPLFTE
                     KFDGEFFYNISNLIDTVSMYTKIDFFKDKVLFNFLFHHIRLSLGVPILFQGENLPESV
                     QILVERNKFLYTVISLLVNDIFPKYLHTEYEYGMIALHFISSLGRSPEIYPVRVLLLT
                     DERRVTRDLLVSKIKSVAPFVELIDIQSLVDYHSIDLSQYDYILSTKPLTNQEIDVIS
                     SFPTVKELLELQERLQYVQAHRTIVARDAIAPEKSYDLQDYLISSSQLLSQFELVQLE
                     NNQSFEHTVEQIIQYQKNVSDRDYLTRKLLSHFQNSPMAIPNTGLVLLHSQSSKVTTN
                     SFTMFELKLPISALSMKREEEEVKRCLLMLMSKEASEEARDLMTAISQSIIENHLYTE
                     IYKTGNQSIIYQMLNTIFNEKIKKLEN"
     gene            complement(4182..5951)
                     /locus_tag="VVL45_000546"
     CDS             complement(4182..5951)
                     /locus_tag="VVL45_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEU4186765.1"
                     /translation="MEEKVSLKVRVQKLGTSLSNMVMPNIGAFIAWGVLTALFIADGY
                     LPNEQLATVVGPMLTYLLPILIGYTGGYMIHGQRGAVVGAIATVGAITGSSVPMFIGA
                     MVMGPLGGWTIKKFDEKFQEKIRPGFEMLVNNFSAGLVGFALLLLAFYAIGPVVSTLT
                     GAVGNGVEAIVNARLLPMANIIIEPAKVLFLNNALNHGIFTPLGVEQVAQAGKSILFL
                     LEANPGPGLGILLAYAVFGKGSAKSSSWGAMVIHFFGGIHEIYFPYVMMKPTLFLAAM
                     AGGISGTFTFQLLDAGLKSPASPGSIIAIMATAPKGVWPHLNVLLGVLVAAVVSFLVA
                     ALILHADKSTEDSLEAAQAATQAAKAQSKGQLVSTSVDAVVSTDSVEKIIFACDAGMG
                     SSAMGASILRDKVKKAGLEIPVSNQAISNLLDTPKTLIVTQEELTPRAKDKSPSAIHV
                     SVDNFLASPRYDEIVASLTGAFPIAEIEGDIPTSAPVDSQEIDLNHIDAVVVAYGKAQ
                     GTATMGCETIRAIFRNKNIRIPVSTAKISELGEFNSKNIMIVTTISLQAEVQQAAPNS
                     QFLIVDSLVTTPEYDKMAARMYK"
     gene            6160..7500
                     /locus_tag="VVL45_000547"
                     /pseudo
     CDS             6160..7500
                     /locus_tag="VVL45_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(7628..8650)
                     /gene="cbpF"
                     /locus_tag="VVL45_000548"
     CDS             complement(7628..8650)
                     /gene="cbpF"
                     /locus_tag="VVL45_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpF"
                     /protein_id="HEU4186766.1"
                     /translation="MKLLKKMMQIALATFFFGLLATNTVFADDSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDPLSGEMVVGWQYIPAPHKGVTIGPSPRIEIALRPDWF
                     YFGQDGVLQEFVGKQVLEAKTATNTNKHHGEEYDSQAEKRVYYFEDQRSYHTLKTGWI
                     YEEGHWYYLQKDGGFDSRINRLTVGELARGWVKDYPLTYDEEKLKAAPWYYLNPATGI
                     MQTGWQYLGNRWYYLHSSGAMATGWYKEGSTWYYLDAENGDMRTGWQNLGNKWYYLRS
                     SGAMATGWYQESSTWYYLNASNGDMKTGWFQVNGNWYYAYDSGALAVNTTVGGYYLNY
                     NGEWVK"
     gene            complement(8669..9490)
                     /gene="cbpG"
                     /locus_tag="VVL45_000549"
     CDS             complement(8669..9490)
                     /gene="cbpG"
                     /locus_tag="VVL45_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpG"
                     /protein_id="HEU4186767.1"
                     /translation="MNVEGRGSANFIKDNVLITAAHNYYRHDYGKEADDIYVLPAVSP
                     SQEPFGKIKVKEVRYLKEFRNLNSKDAREYDLALLILEEPIGAKLGTLGLPTSQKNLT
                     GITVTITGYPSYNFKIHQMYTDKKQVLSDDGMFLDYQVDTLEGSSGSTVYDASHRVVG
                     VHTLGDGANQINSAVKLNERNLPFIYSVLKGYSLEGWKKINGSWYHYRQHDKQTGWQE
                     INDTWYYLDSSGKMLTDWQKVNGKWYYLNSNGAMVTGSQTIDGKVYNFASSGEWI"
     gene            complement(9571..9810)
                     /locus_tag="VVL45_000550"
     CDS             complement(9571..9810)
                     /locus_tag="VVL45_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4186768.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPESNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(9960..10226)
                     /locus_tag="VVL45_000551"
                     /pseudo
     CDS             complement(9960..10226)
                     /locus_tag="VVL45_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(10251..10505)
                     /locus_tag="VVL45_000552"
     CDS             complement(10251..10505)
                     /locus_tag="VVL45_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEU4186769.1"
                     /translation="MAANYLKAMQSYLKETDLPKLEQLVVTKSLWDTVDILDRVVGSL
                     VYEHPELEEIILILFENLFKPRQLYLQPQNSVLSNLRLAS"
     gene            complement(10487..10728)
                     /locus_tag="VVL45_000553"
                     /pseudo
     CDS             complement(10487..10728)
                     /locus_tag="VVL45_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(10729..11361)
                     /locus_tag="VVL45_000554"
     CDS             complement(10729..11361)
                     /locus_tag="VVL45_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4186770.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGQEEF"
     gene            complement(11375..12370)
                     /locus_tag="VVL45_000555"
     CDS             complement(11375..12370)
                     /locus_tag="VVL45_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU4186771.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSMTCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVADPDLDASFKS
                     LSGKLNLLTEALQKAENQSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQTQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(12367..13065)
                     /locus_tag="VVL45_000556"
     CDS             complement(12367..13065)
                     /locus_tag="VVL45_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEU4186772.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLIFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(13142..14152)
                     /locus_tag="VVL45_000557"
     CDS             complement(13142..14152)
                     /locus_tag="VVL45_000557"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEU4186773.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKAVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEAKDQMK
                     KA"
     gene            complement(14136..15143)
                     /locus_tag="VVL45_000558"
     CDS             complement(14136..15143)
                     /locus_tag="VVL45_000558"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEU4186774.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLEICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYDVSVDQELLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKVAAWLEEENLATVLERDWGFSISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLTSSKETVTSLVEALEQGKSEKIIDQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQTVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(15130..16083)
                     /gene="mvaD"
                     /locus_tag="VVL45_000559"
     CDS             complement(15130..16083)
                     /gene="mvaD"
                     /locus_tag="VVL45_000559"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEU4186775.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMAFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGHRYRLIVSKTKDLSQDDCC"
     gene            complement(16065..16943)
                     /gene="mvk"
                     /locus_tag="VVL45_000560"
     CDS             complement(16065..16943)
                     /gene="mvk"
                     /locus_tag="VVL45_000560"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEU4186776.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLNITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(17118..17396)
                     /locus_tag="VVL45_000561"
     CDS             complement(17118..17396)
                     /locus_tag="VVL45_000561"
                     /inference="COORDINATES: protein motif:HMM:NF024736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU4186777.1"
                     /translation="MIRKAYDTDLNDQEWAKIEPYFSKHRTYKWPKRVLVNETLYVTK
                     TGCQWRMLPHDFPLYLMVWSFFRRSMTTGWFQVNGRWYYAYSAQVPWQ"
     gene            17921..18883
                     /locus_tag="VVL45_000562"
     CDS             17921..18883
                     /locus_tag="VVL45_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HEU4186778.1"
                     /translation="MTSSTEFGNFVSNLSFQHGIAGLLFPLNKLYPPELDSKILSIIK
                     KAVTIRTTHTYEYQYSLLFGDAGYLWLLLHLFSISKNQYYLQLANVTAKKLIENYDTL
                     EEIDFALGKSGVLLSLIKYYQFTNDNTLKIFIHNSIGEIYHYFLQRDTAKESILDYSF
                     AHGYCGIAYALFAYSKVLEPSMFYNDLHTFHTELKKLLEKVTSNTENLGNLQLSWCKG
                     ISGIILYLCMYDCDGNKDIISKYQEFVFNHHLKMMTGYCHGITSLLQTTVYNQNKLLM
                     KKIQQVILACSERDDHGLLMFQGDSGKADLFDFGIGSMGYIGVY"
     gene            18926..20158
                     /locus_tag="VVL45_000563"
     CDS             18926..20158
                     /locus_tag="VVL45_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4186779.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALWIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVRIVANLAGA
                     FLINVISIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLLALLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLTLITSILCQAYIVIFMTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(20276..21274)
                     /gene="cbpJ"
                     /locus_tag="VVL45_000564"
     CDS             complement(20276..21274)
                     /gene="cbpJ"
                     /locus_tag="VVL45_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpJ"
                     /protein_id="HEU4186780.1"
                     /translation="MKILKKTMQVGLTVFFFGLLGTSTVFADDSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDSLSGEMVVGWQYIPFPSKGSTIGPYPNGIRLEGFPKS
                     EWYYFDKNGVLQEFVGWKTLEIKTKDSVGRKYGEKREDSEDKEEKRYYTNYYFNQNHS
                     LETGWLYDQSNWYYLAKTEINGENYLGGERRAGWINDDSTWYYLDPTTGIMQTGWQYL
                     GNKWYYLRSSGAMATGWYQEGTTWYYLDHPNGDMKTGWQNLGNKWYYLRSSGAMATGW
                     YQDGSTWYYLNAGNGDMKTGWFQVNGNWYYAYSSGALAVNTTVDGYSVNYNGEWVR"
     gene            complement(21292..22314)
                     /gene="cbpC"
                     /locus_tag="VVL45_000565"
     CDS             complement(21292..22314)
                     /gene="cbpC"
                     /locus_tag="VVL45_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HEU4186781.1"
                     /translation="MKLLKKMMQVALAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIPGTGYRDNLFDNQPVNEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     EGHWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLDASGKML
                     TDWQKVNGKWYYFGSSGSMATGWKYVRGKWYYLDNKNGDMKTGWQYLGNKWYYLRSSG
                     AMVTGWYQDGSTWYYLDPSNGDMKIGWTKVNGKWYYLNSNGAMVTGSQTIDGKVYNFA
                     SSGEWI"
     gene            complement(22413..23102)
                     /locus_tag="VVL45_000566"
     CDS             complement(22413..23102)
                     /locus_tag="VVL45_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4186782.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLMLLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVLIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            complement(23114..24538)
                     /gene="gndA"
                     /locus_tag="VVL45_000567"
     CDS             complement(23114..24538)
                     /gene="gndA"
                     /locus_tag="VVL45_000567"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEU4186783.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVADVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEGQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            complement(24614..26008)
                     /gene="mapZ"
                     /locus_tag="VVL45_000568"
     CDS             complement(24614..26008)
                     /gene="mapZ"
                     /locus_tag="VVL45_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HEU4186784.1"
                     /translation="MSKKRRNRHKKEGQEPQFDFDEAKELTVGQAIRKNEEVESGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTDSN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLATQIKAIQDVNAQFEKPAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQTSSSSSS
                     QASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            complement(26021..27178)
                     /locus_tag="VVL45_000569"
     CDS             complement(26021..27178)
                     /locus_tag="VVL45_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEU4186785.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVDRELELDIMGCDIDARMVEIAKANA
                     QVAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            complement(27263..27643)
                     /gene="rnpB"
                     /locus_tag="VVL45_000570"
     ncRNA           complement(27263..27643)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VVL45_000570"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            complement(27664..27993)
                     /gene="gpsB"
                     /locus_tag="VVL45_000571"
     CDS             complement(27664..27993)
                     /gene="gpsB"
                     /locus_tag="VVL45_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEU4186786.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            complement(28063..28590)
                     /locus_tag="VVL45_000572"
     CDS             complement(28063..28590)
                     /locus_tag="VVL45_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEU4186787.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDELNELAENFSEN"
     gene            28656..29252
                     /gene="recU"
                     /locus_tag="VVL45_000573"
     CDS             28656..29252
                     /gene="recU"
                     /locus_tag="VVL45_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEU4186788.1"
                     /translation="MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            29249..31408
                     /locus_tag="VVL45_000574"
     CDS             29249..31408
                     /locus_tag="VVL45_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEU4186789.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGVYDSTAT
                     IVHDEPYNYPGTDTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSLHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLVYGIGRGAYLPWLPQAGKTGTSNYTDE
                     EIEKYIKNTGYVAPDEMFVGYTRKYAMAVWTGYSNRLTPLVGDGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWNSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(31575..36878)
                     /locus_tag="VVL45_000575"
     CDS             complement(31575..36878)
                     /locus_tag="VVL45_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEU4186790.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATAKENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVEKKEQQGTVTIREEKGVRYNQLSSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKDDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKSPTASTWYRGSRVAAPETGSTNRLSITLKSDGQLNASNNDVNLFDTVTLPAAVN
                     DHLKNEKKILLKAGSYDDERTVVSVKTDNQEGVKTEDTPAEKETGPEVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYSLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKLRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTDKEKMYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRNNATVDDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDEAT
                     YFDEIRTFENNSSMYGDKHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HDPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMEALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVADD
                     FASLTAPAQAQEGLANAFDGNLSSLWHTSWGGGDVGKPATMVLKEPTEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKPKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQSSMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLSSLASDQGKMPDYKQEIA
                     RPETPKQILPATGESQSDTALFLAGVSLALSALFVVKTKKD"
     gene            complement(37179..39161)
                     /locus_tag="VVL45_000576"
     CDS             complement(37179..39161)
                     /locus_tag="VVL45_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU4186791.1"
                     /translation="MKSSKLLALAGVTLLAATTLAACSGSGSSAKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDEQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHIDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNLFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPVLYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
BASE COUNT        11609 a         8722 c         6971 g        12080 t
ORIGIN      
        1 tgttcttgtt tcattttact atatagatca cattcgaaag aattcgttcc aaaattgctt
       61 ggttcgttct tctcttggac gactaaagaa ttgatgggca ttattttttt caacaatttt
      121 cccactgtcc ataaaaataa catgatgggc tacatctcta gcaaaaccta tctcataaag
      181 ctgatcaaaa actcaatctg aggagaaaat ccaataaggc tttccataat aaaggacatc
      241 ctatcaagat ttttgaacac ccgataggct gcctttttcc gattttgaag actttttgtc
      301 cagcttcttt taccttataa ataacaacaa aaatcaaaga caagctagaa agattaccgc
      361 agataactca aacagtgttt tgaggttgca gataagactg acggagtcag ctcaaagcac
      421 tgctttgagg ttgcagataa gactgacgga gtcagctcaa agcactgctt tgaggttgta
      481 gatagaactg acgaagtcag ctcaaaatac tgttttgaga ttgcagatgg atgctaacct
      541 gatttgaaga gattttcgaa aaagattaaa tatactctac aatttgctca atcaattctt
      601 ggtcgtctaa acctgtaact tgtataacaa catctttgac tgattgttta gccaacaatt
      661 cacctaatcg aatactttct tcatcattta catcgcgata gtcaaaggca tagccaactg
      721 ttttaagtag gtttttatat gacaaactga gttctttcaa ttcacgtatc ggccggatga
      781 atcgttcatt atagcctaat tttcggattg gagtacgagc tacgcgacta acctcgtcca
      841 ctatgaaagg gttttcgaat cgttctataa tgactttgtg ataattctcc aattcttttt
      901 tatcaaagtt ccatttggca atcaagagac tccgaatttc agctaataca gattcaatcc
      961 gagatttgat attaggattt tgaagagctt ccaaaattgt cttggcacca taatgcgcac
     1021 caatgtaagc tgaagttgca tgtccagaat tgactgaaaa aagttttcgc tcaataaagg
     1081 gttctaaatc ttcttcataa tgcacatctt ctagacgtaa atctggattt ttaagacgct
     1141 tggtttccac gacccattca ttaaagggct cgaccacaac aaaaagggaa tcttcgtgac
     1201 tttgtgctgg aacaatcctg tctactgcag catttggaaa acctatgtag ttatcagcaa
     1261 atgtcaaacc ttccggactt aaatatttct tgacttcttg ataaagaaat tgagacccgc
     1321 caatcatatt ttcacaggcc ataacatcca atgcctgtgt atttcctgca actcggcgag
     1381 cttcgattcc tttggctaga agttcggcga taaaagggag tatattaggt ccgattgcag
     1441 tagtaataat atccgtcctt tgaatcgctt caatgacttg ctcaggatgt tctttgctat
     1501 taattccagc cacattagtt acttctatac gagactgtcc tttctgtgca atttcaattt
     1561 catacttgcc cttttcattt agagcatgaa ttatctgatt attgacatcc acaaaatcaa
     1621 tatggaaacc atttttaaat agaatttcac ctataaaacc acgaccgata ttaccggcac
     1681 caaaatgaac agaatgtttc attcaatttc tccttttcct atttgaggga catatatact
     1741 agatgacaaa tgacgaaatt caattattca acagcatcaa ataagcgcaa tacttcctct
     1801 tttgactgag catctgctag tttaagaaca ttatctacat ctgcacagaa gatagaaatt
     1861 ttctgaataa tttctaagtg ttcattacca ataccagcaa taccaaaaag aaccgttgcc
     1921 acttgagggt tagatacatt cccaaaatca acccctctag ggacttgaac gactgtaata
     1981 ccagacttga ggacatcctt ttttgctgca tctgttccat gcggtatggc gataaagtta
     2041 cccatgtaaa cagataactc tttatctcgc tcaatcatgg cttcaatata gtcttcatta
     2101 acatatccac cctcataaag aacttgccca caataacaaa tagcttcctc cttgttagaa
     2161 aattgtttat taagcttaat caaatgtttt tcaagtttca tattagttct ccaatttctt
     2221 aattttttcg ttaaaaatag tatttagcat ctgataaata atggattgat ttcccgtctt
     2281 gtaaatctct gtataaagat gattttcaat aatcgactga ctgatagctg tcattaaatc
     2341 tctagcttcc tcgctagctt ctttagacat tagcattagc agacaccttt tgacctcttc
     2401 ttcctctcgt ttcattgaca atgcggagat aggtagtttg agttcaaaca tagtaaaact
     2461 atttgttgtt actttgctag actgactatg taaaagcacc agaccagtat taggaatagc
     2521 cataggacta ttctggaagt gagataacaa ttttcttgtt aggtaatctc tgtcactcac
     2581 attcttctga tattggatga tttgttctac cgtgtgctca aatgattgat tattctccaa
     2641 ttgaaccaac tcgaattgac tcaaaagctg actactagat attaaataat cttgcaagtc
     2701 ataacttttc tctggagcga tagcatcacg cgcgacaatt gtacgatgtg cctgtacata
     2761 ctgaagtcgt tcctgtaatt caagcaattc tttgacggtt ggaaaactag aaattacatc
     2821 gatttcctga ttagtcagcg gcttggtaga taaaatataa tcatactgac tgagatcaat
     2881 actgtggtaa tctactagag actgaatatc tatcaactct acaaaaggag caacactctt
     2941 aattttactg actaataaat ctctagtgac ccgacgttca tccgttaaaa gcaaaacacg
     3001 gactggataa atctctggac tacggcctaa gctagagata aaatgtaggg caatcatgcc
     3061 atactcatac tctgtatgaa gatatttcgg aaaaatatca ttcactaaaa gactgatgac
     3121 tgtataaaga aatttattcc tttcaactaa aatctggaca gattctggca aattttcacc
     3181 ctgaaaaagg atagggacgc ctaaactgag ccgaatatga tggaaaagaa aattgaataa
     3241 aaccttgtcc ttaaaaaagt caatcttggt atacatggaa accgtatcaa tcagatttga
     3301 aatattgtag aaaaattcac catcaaattt ctccgtaaag agcggattgt cctgacgttt
     3361 aatgattaat tcatccaaga tgctcgcaaa atagataatt tcctgaatac tataaaattg
     3421 tgcagtctgc ttagagtaag tttgaaaaat tttttgagaa atttccaaag cctgcttact
     3481 agtattaggt gaattttcaa tgttttgctc ctgacctata agagataaca agatcgcaaa
     3541 aaacatcttc atcctagcat ccatatctgg aaaacctgac agttgcttat taacaatctg
     3601 actggccagc ctagttctat ctgcttctaa aatatcaaag ctcccaaaat taccggttga
     3661 aaaatctgct actgagatac agtttgtcag taaaatagcc aaaaatcttc tcttaccaac
     3721 tgaatcacca gaaatccgat aacctttttg tcgttcaatt ttcagatcaa agtctaaaag
     3781 acgcttatca atatctgaaa tatcctgaat aatagttaca ttactgactt tcgtgcactc
     3841 ttgcaatgct tcattggtga caaaaccgct ctcagtcaga aggcgatagg taatcaactc
     3901 ttgtctttct tggggactat actcaactaa cacttcaagt tctgtcggca aatcatccaa
     3961 ctctccagtc aaaatatagt atttcccatg cttcgttatt tcgattccat attgctccat
     4021 gctatctgtc aaatctgata aagttcgata aattgttcta gatgaaacct gtaagatttc
     4081 agtcatatct tgcattgaaa gcttccctac atgtaggaaa gccttcaata attgttcttc
     4141 tcgtttggtt aatagcattc gtaatttaga aacctctagt tctatttgta cattctagca
     4201 gccattttgt catattctgg tgttgttact aaactatcca caataagaaa ttgagaattc
     4261 ggtgctgctt gctgcacttc tgcctgtaaa gaaatagttg ttacaatcat tatgttttta
     4321 gaattaaatt cacctaattc tgaaattttg gcagtagaaa ctggaatacg aatattcttg
     4381 tttctaaaga tagcccgaat cgtttcacag cccatagttg cagttccctg tgctttacca
     4441 taagcaacta ctacagcatc aatatggtta aggtcaattt cctgactatc tactggtgct
     4501 gaagttggta tatctccttc aatttctgct attggaaaag ctcctgttaa tgaagctaca
     4561 atttcatcat aacgagggga cgctaagaaa ttatcaacag aaacatgaat agcacttgga
     4621 ctcttgtctt tagctcttgg tgtcagttct tcctgagtaa caattaatgt ttttggtgta
     4681 tcaagcaaat ttgagattgc ctgattagat actggaatct ctagacctgc ttttttaacc
     4741 ttatctcgaa gaatactagc tcccatagca gagcttccca taccagcatc gcaggcgaaa
     4801 atgatttttt ccactgagtc tgtcgaaaca actgcatcaa cagaagttga tactaactga
     4861 cctttagact gagccttagc tgcttgggta gccgcctgag cagcttcgag cgaatcctca
     4921 gttgacttgt ctgcatgaag aataagggct gctacaagga aagaaacaac tgctgctact
     4981 aaaacaccta gaagaacatt tagatggggc caaacacctt ttggcgccgt agccataatc
     5041 gcaataatag aacctggtga agctggagat ttaagaccag cgtctaagag ttgaaaagta
     5101 aaagttccag agatacctcc tgccatagca gctaaaaata gagtaggctt catcataaca
     5161 taaggaaagt aaatttcatg aatccctccg aagaaatgaa taaccattgc cccccaagaa
     5221 gaagatttag cagaaccttt accgaataca gcataagcta atagaattcc caggcctggt
     5281 ccaggattag cttccaatag gaagagaatt gacttaccag cttgagctac ctgttctact
     5341 cccagaggag taaaaatgcc atgattgagg gcattattga ggaaaaggac tttagccggt
     5401 tcgatgataa tattagccat aggaaggagg cgagcattga caatagcctc aacaccattc
     5461 ccaacagctc cagtaagagt cgatacgact ggaccgattg cgtagaaagc caaaagcaat
     5521 aatgcaaaac caacgagacc agctgagaag ttattaacta acatttcaaa tccgggacga
     5581 attttttctt ggaacttctc atcaaatttc ttgatagtcc atcctcccag tgggcccatt
     5641 accatagctc cgataaacat aggaacacta gaacctgtga ttgcaccaac agtagcaata
     5701 gctcctacaa cggcaccacg ttggccatgg atcatatatc cacctgtgta accaatcagg
     5761 attggcaata aatacgttaa cataggacca acaacagtag ctaactgttc atttggcaga
     5821 tagccatcag cgataaagag ggcagtcaat actccccaag caataaatgc tccaatattg
     5881 ggcataacca tatttgaaag cgatgtccct agtttttgaa ccctgacttt caatgatact
     5941 ttttcttcca ttagatcatc ctccttttct taaaactagt atatacaaag ttaaaaacct
     6001 ttaaaactaa agttttgtca cagattttgt gtcaaaacat tacttttgac gaggctgggc
     6061 aaaaaggctt aaaaattata aaacgcatca tatcaggtgt acatgaacta caccccaaaa
     6121 gttagacaga aaaaatctaa cttttggggt gtttttatta tgaaactaac ttatgatgat
     6181 aaagttcaga tctatgaact tagaaaacaa ggatatagct tagagaagct ttcaaataaa
     6241 tttgggataa gcaattctaa tattaggtac atgattaaat tgattgatcg ttacggaata
     6301 gagttcgtca aaaaaggaaa aaatcgttac tattctcctg atttaaaaca agaaatgagt
     6361 aataaagtct gacatgaagg ctggactaaa gatagagttt ctcttgaata cggtctccca
     6421 agtcgtacga tacttcttaa ctggctagca caatacagga aaaacgggta tactattgtt
     6481 gagaaaacaa gagggagagt acctaaaatg ggacgtaagc caaaaacgag acctgaagag
     6541 aggacagaat tagaacgtct tcaagcagaa aatgagtacc tgagagcgga gaatgccatc
     6601 ctaaaaaagt taagagaact ccgattgaag gaggagaaag aagaaagaca gaaattgttc
     6661 aagaattaat gactgaattt tcgttagctc ttcttctaaa agtcattaaa ctagctcgtt
     6721 cgacctacta ctatcacttg aaacagctag ataaaccaga taaggactaa gagcttaaag
     6781 ctgaaattca atccattttt atcgaacaca agggaaatta tacttatcgc cgggttcatt
     6841 tagaactaag aaatcgtggt tatctggtaa atcataaaag aattcaaggc ttgatgaaag
     6901 tactcaattt acaagctaaa acgcgacaga aacgaaaata ttcttctcat aaaagacgtt
     6961 ggcaagaagg cagagaatct cattcaaggc caatttgaag gctctaaaac aatggaaaag
     7021 tgctacacag atgtgacaga atttgccatt ccagcaagta ctcaaaagct ttacttatca
     7081 ccagttttag atggctttaa tagcgaaatt atcgcctata atctttcaac ttcacccaac
     7141 ttagaacaag tacaaacaat gttggaacag gcattcacag agaagcacta cgagaatacg
     7201 attctccata gtgaccaagg ctggcaatac caacacgatt cttatcatcg gttcctagag
     7261 agtaagggaa ttcaagcatc catgtcacgt aagggaaaca gcccagacaa tagtatgatg
     7321 gagtccttct ttgggattct gaaatcggaa atgttttacg gttatgagaa gtcgtttcag
     7381 tcgcttaagc aattggaaca agccattata gactatattg attactacaa caataaacga
     7441 attaaggtaa aactaaaagg acttagccct gtgcaataca gaactaaatc cttcggataa
     7501 attaattgtc taactgtttg agggcagtac agaaaaacct tgatactatg cgttttttga
     7561 ttgtgaagac ttttatccac ctattataca aagttaagta tccatcacag tttacaatta
     7621 gccttcatta cttaacccat tcaccattat agtttaagta gtaaccacct actgtggtat
     7681 taacagctaa agcacctgaa tcataggcat agtaccagtt accattgact tggaaccagc
     7741 ctgttttcat atctccatta cttgcattta gatagtacca agtcgaactt tcctgatacc
     7801 aaccagttgc catagctcct gatgaacgga gatagtacca tttgttccca aggttttgcc
     7861 agccagttct catatcacca ttttcagcat ctagatagta ccaagttgag ccttccttat
     7921 accagccagt tgccatagct cctgacgaat ggaggtagta ccatctatta cctagatatt
     7981 gccaacctgt ttgcataatg ccagttgctg gatttagata gtaccatgga gctgctttta
     8041 gcttctcttc atcatacgta agagggtaat ccttaaccca accacgtgct agctctccaa
     8101 ccgtcaatct gttgatgcgc gaatcaaagc caccatcctt ctgtaaataa taccaatgac
     8161 cctcttcata aatccaacca gtttttaaag tatgataact acgctgatct tcaaaataat
     8221 agactcgttt ctctgcttgg ctatcatatt cttccccatg atgtttgttg gtattcgtag
     8281 cagtttttgc ttctaaaact tgcttgccaa caaattcttg taatacacca tcttgaccaa
     8341 aataaaacca atctggtcta agagcaatct ctattcttgg agaaggacca atcgtaaccc
     8401 ccttgtgtgg agcaggtata tattgccagc cgacaaccat ctctccggat aaaggatcaa
     8461 aataatagta cttcccatct atcactctcc agtaggtttc ttttagatcc ccctttttgt
     8521 agtaggttct accattttct tggacaaact gccatccttc agaatcatct gcaaatactg
     8581 tatttgtcgc taacaaaccg aagaaaaatg tggctagtgc gatttgcatc atttttttca
     8641 aaagcttcat tttatatatc ctccaacatt aaatccactc accagatgaa gcgaagttat
     8701 aaactttacc atcgatagtt tggctacctg taaccattgc tccatttgaa ttgagataat
     8761 accattttcc atttactttt tgccaatctg taagcatctt accggaactg tctaaataat
     8821 accaagtatc atttatctcc tgccaacccg tttgtttatc atgttgtcta taatggtacc
     8881 aactaccatt tattttcttc catccttcaa gagagtaacc tttaagaacc gaataaataa
     8941 atggcaaatt tcgttcattt aatttaactg cactgttaat ttgattagct ccatctccta
     9001 aagtatgcac tcctactaca cggtgactag catcataaac tgtagatcca ctagacccct
     9061 ctaaagtatc aacttggtaa tccaagaaca tgccatcatc acttaaaact tgttttttat
     9121 ctgtatacat ttgatgaatt ttaaaattat atgatggata gcctgtgata gtcacagtta
     9181 ttcctgtcaa atttttttga ctagtaggaa gacccaaagt ccctaatttt gcaccaatgg
     9241 gctcttctag aattaataaa gccaagtcat attcccttgc atccttagaa tttaaatttc
     9301 taaattcctt caaataacga acttccttta ctttgatctt tccaaatggt tcttgacttg
     9361 gactaacagc cggaagaaca taaatatcat ccgcttcttt cccatagtca tgtctgtagt
     9421 agttgtgagc cgctgtaatt aacacattat ctttaataaa attcgcactt cccctccctt
     9481 ctacattcat tccatcggct actccataat acttagataa aaccatagct gaagtcgaat
     9541 acggatactg taaagtatta tcaattttaa tcaaatcatc attaccgata atacttctga
     9601 ttgcttttgg tagtatgaac catacgttgg tgaaatctca gataatgaag aatcattaga
     9661 ctctggacct ttttctagtg tctcacttac ctcatattct tcacctgtac tagaaataac
     9721 actcaaagca gatactgtcg ataactggct agccaataaa gtactcgcaa taattgaaat
     9781 acccaatttt ttataaacag ttttcttcat tattgtatcc tcctaatgta attatagcgt
     9841 actattctaa atttcttaat ctactataga atcaagaaat ctaccacctt ctttaaatac
     9901 cctccattat cacataaaca ggtaaacttt tcaattaatg actgcgcttt tcaatcacgc
     9961 tagaggtact tgcttgcttc tttgatacta agttcagcca ttctttcctt gtttttctca
    10021 ataaagcatg ttacccaagt gggattcgtt ttggagtagt ctcgcagagt ccagccaatg
    10081 gctttattga taaaaaattc tgtttggttc aagttatgaa ggagaatctt ttccattaat
    10141 tgagtattgg tcttctcttt tcttaacaac tggtggtcaa tagcgacacg tctcagccag
    10201 atattatctg ataggctcca ttttatactc aatgaaaatc aaagagcaaa ctaggaagct
    10261 agccgcaggt tgctcaaaac actgttttga ggttgcagat agagctgacg tggtttgaag
    10321 agattttcga agagtattaa gattatttct tctagttcag ggtgttcata caccaaactc
    10381 cctactactc gatctaggat atctaccgtg tcccacaagg attttgtcac gactaactgc
    10441 tctagcttag gcaaatcggt ttcctttaga taagactgca ttgctttcaa atagttagca
    10501 gccacatatt ggtattttct aggatccttt tcccagcaag tgtctgcaaa atcccaatcg
    10561 ataatctttg tttttttcgc ttctggaaaa tattttatag agtttatttc tttcaggcac
    10621 cgcaatacct agaaaagaaa attgatggcg catataggct tccatggacc ttgctttttt
    10681 agagtctttt gctgcttcta gctcctcaag taaatctgct aaactcatct aaaactcctc
    10741 ttgccccacc aaatggtgct gaaaggcata gacagccgcc tgggtacgat cgctgacttc
    10801 aagtttggca agaatattgg acacgtgggt cttgaccgtc ttgagagaga taaagaggtc
    10861 atctgcgatg cgctgatttt cgtagccctt ggcgatgagt tggagaacat ctcgctcacg
    10921 cgcagtcaat tcttcatgaa gttccatatg attgcggtgg tattcaacct tcttgctaac
    10981 ctcttgctca atggccagct cgccagcagc taccttactg acggcatgaa gcaattcatc
    11041 tgcactagaa gtcttgagca tatagccttt ggcaccagca tctaagactg gcatgatttt
    11101 ttcattgtcc aaataagagg tcacaatcaa aatcttggct tcaggccatt ctttaaggat
    11161 tgctaaggtc gcgtcaatcc cattcatctc aggcatgaca atatccatga caatgacatc
    11221 tggacgcagt tccaaggcca agtcaatccc ttgagacccg ttggacgcct cacccacaac
    11281 ttctacatcg tcttggaggt caaagtagct tttcaagccc aatcggacca tttcatggtc
    11341 atctactagt aaaattttca tctttactcc tttatcattc cttatctaac aggggaatac
    11401 ggatatcaac cgccagccct tgcttgggag ctgtcaagag ttgaactgtt ccagccatat
    11461 cttcaacccg ctccttgata tttcgcagtc cataactcaa gtcgtctaag ctccctaact
    11521 ggaaaccaat cccattgtcc accaccttca gttgcaattc aacatctgtc tgatagaggt
    11581 agacatctag gcaagatgcc tgggcatggc ggagggtatt gctaatcaac tcttgcagga
    11641 tacggaagat atgctcctcg attttcttag gcaatttcgt catattctgc ttgagactaa
    11701 ccctaagatc actcttgtcc tcaagctctt ttaaaagaat ttgaatccct tctatcaagc
    11761 tcttctgctc cagttcaact ggtcgcaaat gcaagagcaa aacccgcaaa tccttctggg
    11821 ctgtttctaa aatagctgtg acactctgca actgggtctg catcttttct ctatccaatt
    11881 tcaaagcctg ctgactgata cccgataaaa tcatgtgggc cgcaaacaac tcctgactga
    11941 ctgtatcgtg caaatcccga gcaattcgct tccgttcctt ctcgatgatt tcctcttcct
    12001 gagcaaggct ctgattttca gctttttgaa gagcctctgt caaaaggtta agtttacctg
    12061 ataaggactt gaaactggca tccaaatctg gatctgcaac ctgaaccact tcttgccctg
    12121 ctaataaacg cttgagatta gcctgcattt ttcttagaga aagctcttcg atccctcgcc
    12181 aaaacagggc taagagacag gtcatggaca tgctgaaaac caacaataaa aagacaaatt
    12241 tttctgtttt ttcgacatcg tgcaaaaaga tagaccagtc aaaatcaagt atttccagca
    12301 agctgtggga gaaaaaaaag acaaatagga aggaggtgag agcaataatg acataggctt
    12361 gttttttcat cctctaacca cctccacatc accaatcata gtggtcaaga aaatcttgac
    12421 actcttgtta ctcttgagat agtcttttgt ttcttgatga tagtgttcat tgcggagggc
    12481 tcgcttgggc tggttgaaaa aaatcaaatc cccatagaga cagttaacgc tgagactgac
    12541 ttccacatct acaggtacga tgattttggt cgttcctacc atctttctga ggataatgac
    12601 attgtcatga ttggttaaga tgaccctctc cagatgaata gtgtccttgc ccatgaagcg
    12661 aaagagattg atatcatcga attggcaagt ctggtagctt gaaaaatgat gaagatttcc
    12721 aaaccaacga tttttctcct tcttaaccgt cacgacctct tcaaaaacca aattggtctg
    12781 ctctttttcc tggttcatca tcgggtaaag aagaaagagg ctatagataa ccgcaacaaa
    12841 aatagctaga atcacaaaag gattgagcat aacgatgaaa aagaagagaa tggttgccgc
    12901 tactaaaaga agattatttc cctctttacc agtgtagtag cgaatcaaaa gcaaaaagag
    12961 gaatagtatc agcagaaaac gcgaaaaatg ctctgatacc atcaaaatca gagctcctgt
    13021 cagaagacag gcttcgataa ataaaaagat tttaaatttt ctcataggtt catcctctcc
    13081 cttctatttt atcacaattc aaaaaagtca cctcagtctg aggatggaaa aaaggcgctg
    13141 gttacgcctt tttcatctga tcctttgctt cttttaattt tccataaaga agatagtcta
    13201 ctttttgtag atctgctatg gtggcacagt taagggaaca cataatcaag cgtagatctg
    13261 ctttccagcc ttggacaatg ccaatcactt cttcaactgt gtaggtttca accaattcca
    13321 gaacggttcg tgacaatccc acagccttag caccaaaaac caagcactta atcatatcca
    13381 gcggattccg aacccctcca ctaaccaaga gttcgacctt atctttccat tcttgggcat
    13441 tgagaagggc ctgcatggta gactgacccc attgattgag gtaatcacgc tggccactac
    13501 gacggttttc gatataggca aagctggtgc caccacgacc cgataggtcc actgtacgaa
    13561 caccgaattc ataggctctt tcgattgtct tggcatccat tccaaagccc acttccttga
    13621 ggacaatagg aacgggaatt tgcttgctat aatctgctag atgcgattgc cagcttctaa
    13681 acttcctttc tccctcgggc atgagtaatt cctgcatgac attgacatgc acttgcaata
    13741 gaacaggatt catctcttct acagtctgaa gtcctaactc gacaggcttg tccaatccaa
    13801 tattggttcc aaggaggaga ttgggatgac tagacttgac agaaaaagaa tcatccgttg
    13861 gatttttgag ggctgcgcta taagaacccg ttacaaataa aataccacag gattccgcca
    13921 cctgagccag cttttgattg atttctcttc ccttattact tccaccagtc atggcattga
    13981 tataaaaagg aaagtcccac tttcgaccag caaactctgt cgaaagatcg atttcatcca
    14041 gattgtaaag aggcaaggaa gaatgaatca gctccacctc atcaaagcta ttataggaac
    14101 ttttctgctc aagggcatag aggatatgct cgtccttacg atttgtcgtc atgtcctatc
    14161 ctttcttgat ataagagctc aatccccaga tcggcccaac gattttttaa ggttttggtt
    14221 gattgcgcat caaaactcag ggcgatgcca cagtcaccac caccagcacc actactcttg
    14281 gcaacggtct gcaaatcttg actggcttct ttcaactgtc taagcaaagg cgtgtaaata
    14341 tctgtactca agccttctaa aagcttgctg gctacttcta cttgatcgat aatcttttct
    14401 gatttcccct gttccaaggc ttctaccaga gaagtcaccg tttcttttga ggaagttaaa
    14461 aaattttgat tgatattttg cttgatttgc tggaccatgt gactcgatac agccacttcc
    14521 ttggtccatc ccactaagaa atcacattct aaagttggtt tcacttgtga aattgaaaag
    14581 ccccaatcac gctccagaac tgtcgccaag ttttcttctt ctaaccaagc agccaccttc
    14641 tggcgatcaa atgactggta gagaaccaaa tcctctgcca caatacaggc aaggtcgccc
    14701 atggaaccat tgtctcctcg cttaagcaag acagcgctag tcagcttgaa caagagctcc
    14761 tgatcaacag aaacatcata cagagccagt aaagccttga caaccaagac aacgacgctg
    14821 ccactagaac ctagaccaaa ctttttccct tctcgttcca ttttgccaca gatttctaga
    14881 gaaaaaggtc ttaaattctg accacgaaca gcgaggaagt ctcccatcaa agcaatcgtt
    14941 tcttgaatca agctatagtc aggattaggc cttaagtcca ctgcgaaatc aaacatatct
    15001 gaatagatac ggtagctgtc agaaaaagca atctcagccc tcatatagat gggaatatcc
    15061 tttatcaaag ctaactgccc tggctctaaa atagcatatt cacctgccca atagagtttt
    15121 ccgcaagttt taacagcaat catcttgact caaatccttt gtttttgaca caatcaagcg
    15181 ataacgatga ccgaaaattt ctgataaatg ctccaagtct ttctcctgac agaagacctt
    15241 aacattggga ccagcatcca tggtaaagta gcaggcctct cctttctcac gaagctggcg
    15301 aacaaaggcc atagcctcat aagaggcatc cgtcagataa gaaaaggctg gactagcagt
    15361 ctttgtcgta gcatgcatag ccagggcatt tttctccgtt aattctccaa tcttggcaaa
    15421 atcattttcc ttgagataaa tcagcatatc ctgatagtcc ttctcagact gacgaaccca
    15481 gtcgtcgaaa gtcgtcgagg tttccacaca aagtttcatc ccgtcacggc tagagattgg
    15541 ttttttcttg tcctctagca ccaacataat catagctagt ttcaagtctg tctctacagg
    15601 gtaaatttct ccactatcct tatcccaggc tcctagtggt ccataaaaac tccgagaaga
    15661 agaacctgag gcaaatttgg cttcctgtgc caactgactt ctatccaatc caagcttgaa
    15721 ataagcatta caagccttga ccagggcgga caaaccacta gaacttgagg acagacccgc
    15781 tgccgtaggc atattgtttt gagtatcgat acggacaaag ccctcaccag ctggacgata
    15841 acggtcaata atcttactca tcttggcatg ctcgacctca ttttgtagct gaccattgat
    15901 gtaaaattcg tcagctgtta cattggctgg taaaggcgac aaggtcgtct ctgtatacat
    15961 attttccaaa gttagagaaa tactgctagt agcaggcacc atctcttttt cttttttctt
    16021 tccccaatat ttgataatag caatatttgc gtaggaacgt actgttacag gctctctatc
    16081 catgtctgaa cagctccttt ctcttctaat ctttctgcta gttcttgtgc gtgtgtcaaa
    16141 ttggttacca aggctatgat acaacctcct agcccaccac cgctcatctt ggcacccaga
    16201 gcaccatggc taagagtcgt ttcaaccaaa aagtctgcct cagggctact gactccaatt
    16261 tcttttaaat gtaaatgcgc ttgactgagg atttgtccca gtccttcagc atctttttgt
    16321 gaaatcgcaa cttctgcttg ctgggttaat tctcccaagg catgcaaaaa cggtagggca
    16381 tccttgccct tattttgaac cacttggatg gcttcacgag tatgaccata aacacccgta
    16441 tcggcaatca ccaaataggc ggataaatcc atctcaagtt ctgtaaatcc tacgttcttg
    16501 ataaagcgaa taggttggtc actaagacag gtcttagcat ccaaaccact aggattcata
    16561 tgggcaatca tttcagctcg attgaccaag atttctagta catcatgagg cagatcagcc
    16621 tgatagtagt caaatactgc acgaatggcc gctatgctga tagccgctga cgaacccatc
    16681 ccccgtttct cagggatagc cgagtcaatc tcacaacgaa tgcaggcttc tgtgatattc
    16741 aaatactcca gtgaggcata aaccgccatg gacaaggtat cctcctcata aaggcgccaa
    16801 ggactctctg caggaactac cttacaggtc acctccacct ccaaaagagg cagggaaatg
    16861 gcaggataac cgtaaacgac cgcatgttcc cctattaaaa ttatcttact atgtgcctga
    16921 ccgacaccaa ctttttttgt cattttttcc ttttactaga cgaaaaaacg tcttattttt
    16981 catacaagta ttaattcttt cctatctatt ttattatatt ttcacaaaaa aagcgattgt
    17041 ttccattcac aatcgcttct ttcattattg aacccattcg ccattatagt tgacagaata
    17101 gccatctacg gtcgtattca ctgccaaggc acctgagcgc tataagcgta gtaccatctg
    17161 ccattgacct ggaaccaacc tgtcgtcata gaacgacgaa agaaactcca taccattaag
    17221 taaagaggaa agtcgtgagg gagcatgcgc cattgacaac ctgttttagt gacgtacaaa
    17281 gtctcattaa caagtactcg tttcggccat ttataggtgc ggtgtttgga gaaatagggt
    17341 tcaatcttcg cccattcttg atcgtttaaa tcagtatcat atgctttgcg tatcataact
    17401 ctagcttaac atttttttgt gaatacaggt tctaaataat cgaccacgaa aatttcttaa
    17461 gtggaaaacg ccttatgaag tatgctacgg gaaagttatg cacttaattt gacaattcaa
    17521 gatgtaaaaa tatatactat agtagattga aactagaata gtacacctct acttctaaaa
    17581 tattgttaga aatcgatttg actgtcctga tcgatttatc ctgttattat ctcattttac
    17641 tataatattt gataagttat cctaaaagta ttattatgtt gttgtgttat agattgattg
    17701 aatctaacta aaggatccta ttcaattact agaactatca catactcaag gtcagctcac
    17761 agatgagcaa ctattttggt tacaatgtct actaaattta agtcaaacaa ataatttagt
    17821 caaaattaaa aaaatagagg aacataaata tgattacaaa acagaatgta atagtgttct
    17881 acaattttta ctagataaaa ctgtaaattc tgaaggaagg atcacttctt caacagaatt
    17941 tggaaatttc gtaagtaatt tatcattcca acacggaata gctggactac tgtttcctct
    18001 aaataaattg taccccccag aactggattc taaaatactc tctatcatca agaaggcagt
    18061 gacaattaga acgacacaca catatgaata tcaatactca ctgctatttg gtgatgcagg
    18121 ctatctatgg ttactcctac atttattttc tatcagtaaa aatcaatact atctacaatt
    18181 agcaaacgtc accgctaaaa aattaataga gaattatgat actctagagg aaatagactt
    18241 tgcattggga aaatctggtg tcctattatc attaataaaa tactatcaat ttaccaatga
    18301 caatactctt aaaattttca tccacaatag tataggggaa atttatcatt atttcctaca
    18361 aagagataca gccaaagaaa gcattttaga ctatagcttt gctcatggat attgtggaat
    18421 tgcatatgct ttatttgcct attctaaagt cttagaacct tctatgtttt ataatgatct
    18481 ccatacattc catactgaat taaaaaaatt attagaaaaa gttacttcta atactgaaaa
    18541 tttaggaaat ttacaacttt cttggtgcaa aggaatttcc ggaataatct tatatctttg
    18601 tatgtacgat tgtgacggaa acaaagatat tattagtaaa tatcaagaat ttgtttttaa
    18661 ccatcatcta aaaatgatga caggatattg ccacggaata actagcttac tacaaaccac
    18721 tgtctacaat caaaacaaat tactgatgaa aaaaatccaa caggtaattt tagcatgttc
    18781 tgaacgagat gatcacggtt tactgatgtt tcaaggagat agtggtaaag cagatttgtt
    18841 tgacttcgga ataggaagca tggggtatat tggtgtctat taaataataa attcccattc
    18901 gatgtgcaga cataaggaga aaagtatgaa attattttgg acaaacaaca tatatagaca
    18961 gttgctgcta aacagctgtt tttcatcatt cggcgacagt attttctacc tcgccattat
    19021 caattatgtg gctcagtaca atttcgctcc gctagcgatt ttactgattt ccatttcaga
    19081 gatggttccc ctactatcgc aactctttct cgggattcta ggagattttc aagaaaatag
    19141 agtcaaacac gcactctgga ttgccaaaat caaaatcctg ctctacgcta ttttgacagt
    19201 atttctcgtc ttgtcgccct tttcattagt ttcagtcatt atgattgtca tcatcaacct
    19261 catctctgac accttgagct acctgtctgc ctacatgatg aacgccctct acatcagtgt
    19321 aattaaggac gacctgcatg atgccatggg gttcaggcag tctctgatga gggttgtccg
    19381 tattgtcgcc aatctggctg gcgcattcct tatcaatgtt ataagtattc aaactatttc
    19441 ccttatcaac actctgactt ttgtcattgc ctttttgggc ctgtatgtta ttcgacatac
    19501 cttgtatgag gttgaaaaaa gaattgaaat gtcacataca gcactgagtt ttaagaaata
    19561 ttttcaacat cttaaacagt cgctggctgt gctcctgagg ttaaaagata ccgtcatact
    19621 actgtttctg acgaccagta tgattgccat cttggatgtg tcccctcggc tgattgccct
    19681 ccgcttcatc caacagacac tagcacaact gagcattggg caactcctcg ccctgctctc
    19741 catcatcatg tcttgtggag ctatccttgg caatatgacc agcagtaatc tatttaaaaa
    19801 tatccgtttc acgcacctct tggttttctg tgagatttcc ctattgactc taataactag
    19861 tatcctttgt caagcctata tcgtaatttt catgaccagt ttcatcagtt ctacgattat
    19921 cggcattctc agccctcgcc tacaagcagc tgtctttgcc catatcccca gtgacaagat
    19981 ggggacggtg ggctctgctc tgagcacagt ggacattctc gccccgtccc tgctctccct
    20041 attagcccta tccatagcat cgggcgtttc ggtgcagtta gcattgatat ttttgtatct
    20101 tattttaatt gctcttatct tttgtcaatg gttagtcaag ttcaacactc ataactaacg
    20161 aaaaagcatg tgtagatttc acatgctttt aatctcccca atcgtcaggt caagtacaac
    20221 aaagtcactt ctttgattaa gcgagtgttc taatataatt ataagcgccc tgtcattacc
    20281 gaacccattc gccattatag ttgacagaat agccatctac ggtcgtattc actgccaaag
    20341 cacctgagct ataagcatag taccagttgc cattgacctg gaaccaacct gtcttcatgt
    20401 ctccattacc tgcatttagg tagtaccaag ttgaaccatc ttgataccaa ccagttgcca
    20461 tagctcctga tgaacggaga tagtaccatt tgttcccaag gttttgccaa cctgttttca
    20521 tatcgccatt tgggtggtct aaataatacc aagtggtacc ttcctgatac cagccagtgg
    20581 ccattgctcc tgatgaacgg aggtagtacc acttattacc tagatattgc caacctgttt
    20641 gcataatacc agttgttgga tctaggtagt accaagtcga atcatcgttt atccaccccg
    20701 cacgtctttc accaccaagg tagttttctc cattaatttc cgtcttagct agataatacc
    20761 agttagactg atcataaagc caacctgtct ctaaagaatg attttgatta aagtaatagt
    20821 tcgtataata acgcttctct tctttatctt ctgaatcttc acgtttttcc ccgtactttc
    20881 ttccaacact gtctttagtt ttaatctcta atgttttcca accaacaaac tcttgtagca
    20941 ctccattttt atcgaagtag taccactctg actttggaaa accttctaat ctgataccat
    21001 ttgggtaagg accaattgta ctacctttag atggaaacgg gatatattgc cagccgacaa
    21061 ccatctctcc agatagagaa tcaaaataat agtacttacc atcaatcact cgccagtagg
    21121 tttctttgag gtcccccttt ttgtagtagg ttcttccgtt ttcttggaca aactgccatc
    21181 cttcagaatc atctgcaaat actgtactgg tccctagcaa accaaagaaa aatactgtca
    21241 gtccaacttg catagttttt ttcaaaattt tcatctatat accctccaat attaaatcca
    21301 ctcaccagat gaggcgaaat tataaacttt accatcgata gtttggctac ctgtaaccat
    21361 tgctccattt gaattgagat aataccattt tccatttact tttgtccaac ctattttcat
    21421 atctccatta gaaggatcta aatagtacca agttgaacca tcttgatacc agccagttac
    21481 catagctcct gatgaacgga ggtagtacca cttgttacca aggtattgcc atcctgtttt
    21541 catatcacca tttttattat ctaagtaata ccatttgcct cgtacatatt tccaacctgt
    21601 tgccatagaa ccagaggagc caaaataata ccattttccg tttacttttt gccaatctgt
    21661 aagcatctta cctgaagcat ctaggtagta ccatggagca ggtttgcttc tatcaatgtc
    21721 aatagtaaca tgaaaatctt gagtccaacc cttagcaact tcaccaattg gtagtggatt
    21781 gtaagaatca tcgccaaaat ttcctagctt atttaaataa taccaatgac cctcttcata
    21841 aatccagcct gtctttacag cataattatt atcaaaataa taagttctct tttcagcaga
    21901 tagaggatat tgttcaccgt atacttttcc tgtattttca gacgtttttg cctctagtac
    21961 ttgtttatct gtttgttcta gcaaagcacc atcttgtcca aaatagtacc acttctcctg
    22021 aaggccaatt tcattaactg gttggttatc gaataaatta tcacgataac ctgttccagg
    22081 aatttctaag tattgccaac cgacaaccat ctctccaata tcaccaaaat agtaagtttt
    22141 accgtctatt ttatgccaat agattgcttt atgatcatct tttacataat attttctatt
    22201 atccttatca acaaatcggc cacctgtggt attcgcaaat accgtatttg tagctagcaa
    22261 accaaagaaa aagactgcta atgcgacttg catcattttt ttcaaaagct tcattttata
    22321 tatcctccta aaacgattgt agtaaaaact agattgtatg tcaatatata aaaactcctc
    22381 aaaaaagcag ttacacaact gcaactgctt ttctattctt gcatggtata tccaacacca
    22441 cgcacagttt taatgtagct tttttggcct ttaacatcaa gcttgctccg tagatagcgg
    22501 atatagacat ctacgatatt tgtctcggtc gcactttcat acttccaaac actttccaac
    22561 aattgctcac gagtcaatac tttcttgctt cccataagtg tcgccaaaag gtcatactca
    22621 cggcgtgtca gagcaatcat ctcttcacca cgataaaccg tgtgatgttc aacatctatc
    22681 ctaagattgc ggtaggtcct tggaactttc atcagactgc agtgttgatc aatgaagtcc
    22741 cgacctcgga agatcgccga aatacgcgct accagatttt cgataaggac tggcttatag
    22801 atgtatgaaa ctgcaaaacg ctgaacaact tccagctctt cttgcaagtc ttcccaatga
    22861 tctaaaatca tgatgactga ggcaggttta gttcggctca atttttctgc aaaatcctga
    22921 gccatcatat ctcccagatt aacgttcaat aacatcaaat catagtctgt ctgaagagcc
    22981 atggagaggg ctttttgccc ctcctctacc agatcaaccc gatactgctc tttctggagt
    23041 tccaaactta aaaaatgagc tagatttcgt tctttctcaa gtaataaaat ccgtttcccc
    23101 atggctgacc tacttatttt tcgtcatacc aagagtagtg gaaggttcct tctttgtctt
    23161 tacgttggta agtgtgagca ccaaagtagt cacgttgtgc ttggatcaag ttagctggaa
    23221 ggtcagctga acggtagcta tcaaagtaag taatagctgc tgagaaagtt ggcactggca
    23281 cacctgcttg aaccgcaaga gctacgatat cacgtactgc ttgttggtac ttagcagtaa
    23341 catccaagaa gtactcgtcc aaaagaaggt tggcaagatc tgcatcgcgg ttgtaagcat
    23401 ctgtaatctt ttgcaagaaa cgagaacgga tgatacagcc atcacgccag atagatgcga
    23461 tatctgcaaa tggcaagttc cagttgtttt ctttagaggc tacacgcaat tgagcaaatc
    23521 cttgtgcgta tgaaatgatt tttgagaagt aaagggcttg acggatcttt tcaatcaatt
    23581 cagccttgtc tccttcaaag ttgaaggcag ctggttttgg aagcaccttg ctagcatgta
    23641 cacgttcttc tttgtaagtt gaaatgtagc gtgcaaacac tgactcagta atcagtgaca
    23701 atggtacacc aaggtcaaga gatgattggc tagtccattt accagttccc ttgttacctg
    23761 cagcatcaag gatgtagtct acgattggtc catcttggcc ttcatcgtct ttacggctca
    23821 agatatcagc tgtgatttca atcaagtagc tgtctaattc acccttgttc cactcagtaa
    23881 agatttcagc catatcttct gcagaaaggc ctagcaagtg ttgcatcaag tcatagcttt
    23941 ctgcgatcaa ttgcatatca ccgtactcaa taccattgtg aaccattttc acatagtgac
    24001 cagctccatc aggaccgatg taagtcacac atggtttgcc atcttctggt gctttagctg
    24061 agatttcttc aagaacatcc gcaaccaatt cgtaggcttc tttttgtcca ccaggcatga
    24121 tagaaggacc ttcaagggca cctttttcac caccagaaac cccagtaccg ataaagttga
    24181 taccagagtt tgccaattct tcattacgac ggatggtatc tttgtagaaa gtatttcctc
    24241 cgtcaatcaa gatatcaccc ttgtcaaggt gtggaagaag ggcttggata gtagcatctg
    24301 taccaggtcc agcttgaacc atcagcatga tacgacgagg tttttcgatt gagtttacaa
    24361 aactttcaac gtcatagctt ggtacaaagt tcttttcagg atggcaagca atcacatctt
    24421 ccgttttttc tttactacgg ttgtagatag caactgtgta accacgagat tcaatattaa
    24481 gggcaaggtt acgacccatt acggccatac ctacgacacc aaagttagct tttgtcattt
    24541 gacactcctc ttgtttcatt tgttttattt tatcattttt acttttgaaa agaaagaatt
    24601 ttgtaacgac tgcttagtaa tctaagtcat ccgcatgacc agcgccattt ccgacaaagt
    24661 agcctgtctt acagttaagg gtaaagagat aggttccatc tggcttgtag aggttgtaat
    24721 aaccattgcc gttaacgata ttgacacgtt caaggatata ttggtctcca gtgatatagc
    24781 cacgtgaacg tgaagtcgct agaatttgtt ccaagacacc atccgcaaaa tcccaggcag
    24841 agttattact atcatcaata gcagactgat tataggcaac acgactggca cttctttgaa
    24901 cagcaacccc tgcactcgag agacccatat tgacttcact gcgactactt ctagtctcat
    24961 ttgaactact tggttttggc tcactagtcg tatttgaact tgcttgactt gaacttgagc
    25021 tgctagtttg acttgaactt gagctagaag tacttgtttg ctggctctta ccaagactga
    25081 tagccttatc tagcacttta tcaagctccg tatttccagt tttaatatcc gtaaatttag
    25141 catccgattt ggctttggca ttggtatcca acacaccatc cacaatagct ggtttctcaa
    25201 attgagcatt gacatcttga atagccttga tttgcgttgc tagactatca tatttagatt
    25261 tggcaagcgt atgttcacga ctaccttcta gcttatcaag taaagtcttg agttgactca
    25321 gtttatcaaa ctggctattt ttcaaagccg ttttattgct atctgtgtaa aaggcgtcat
    25381 aaagtgtatt aaaatcatcc acatccgatt gattggctgt agttgattga gaagtttcaa
    25441 tttccttagt cgaacgagcc acttgacggt agacataata agcactgaca caaataatta
    25501 ctgatacaag cgccaaaacg gtcaaaataa agcctttttt acttttcttt tctgagtcct
    25561 cttgatccag acgagaaagg ttttgctcct cttccacttc ttcagtcatt tctgttggat
    25621 tttcatcatc tagcaataga ggatccaagc cttgctcctc atcgtccaga ggcaagggta
    25681 gtaagatgtc ttcagaagat ggaacttcct ccgaagaagc ttctgacttc tctacagcct
    25741 cacgcatctc ttgaattaaa tcatccagac tctgagtttc aacgaactcc tcttttttgt
    25801 attgacgagt cgcaaactta tccgcctcaa tttcatctct gtgttgctta acatacttgt
    25861 ccaaaatgga atcctcaggc aagactcctg attccacttc ttcattttta cgaatagctt
    25921 gaccaactgt tagctctttt gcttcatcaa aatcaaattg cggttcttga ccttcttttt
    25981 tatgacgatt tcgtcttttt ttactcatga gtatcccttt ctattttacc tcttgccgtt
    26041 tgacacgctg accaaaatat tgatatagat ccactttcaa ggttccgttg tataacttac
    26101 gcttcttatc tgcttggcta ccatacttgc tttcaaaagc ttcatcacta gtcaggataa
    26161 atttgctcca agttttcagc ggtgcaaata cttgccccat ctcagcatag agcttggtca
    26221 cccctgcatc atctgacaaa cgttcaccat aaggcggatt ggaaatgatt actccattga
    26281 ttttatcgga acgtaaatcc tgcacgcgca tctgcttaaa agtaatgtct cctgcaacac
    26341 cagctacctg agcattggcc ttagcaattt ccaccatgcg agcatcaata tcacagccca
    26401 tgatatccag ctcaagctca cggtctactt ttttagccgc ttctgtgcgc acttcttgaa
    26461 tcaagcgatc gctgatccag ttccattcct caaatgcaaa agagcgacga agacctggcg
    26521 ccatctttct agcaatcata actgcctcaa tacagaaagt ccccgaacca caggtcggat
    26581 caatcaaagg cttgtctgga taccagttag aaagttgtaa aatggctgct gccatatttt
    26641 ccttgatagg agcgccacct ttttcggtac gataaccacg tttaaagagg ctagacccgg
    26701 tcgtatcaat catgacagtt gccacatctt tgagaataga gacctcaatc ttaaactctg
    26761 ggccattctc catcagagga accccttctg ggcgagcata gtgtttctgc aatttcttga
    26821 caacagcttt cttagaaata gcctgaacac tgggctcatt gtgaagtttg gacttaacac
    26881 atttagcttt tgaaatcggg aaccgagctc caagtggtaa ataattttcc caatccaaag
    26941 cgaaaactcc ctgaaatagc tcttcaaaag tcttagctgg gaacgttcct acgataattt
    27001 tgatacgatc tgctgcccga agccaaaggt tggtttcgat aatagctctc acgtctcctt
    27061 gaaaacgaac acgtccattt tcaacctgac aatcgtagcc caactctcgc acttcacgac
    27121 caacgacagc ctcaagccct gctgccacag ttgcaattaa attaaattct tttttcatgt
    27181 tacagtcttg caagaccttt ctatatgtcc actttaaaaa atgaggttga gaaaatttct
    27241 cagccccaac ctatatgcaa ttttctataa gccatgtttt gttccagaag tgactaggac
    27301 cacttccttc gataatcatc tgtctaccac taacagctct agctgatagc agtcagaata
    27361 ctacgttcgt ttccgcgtac tccatgcccc gaccaaaatt tgggttgcta gcttgagggg
    27421 tttaccgcgt tccactctct ctgtttccag aaagactccg tcactgtggc actttcaagc
    27481 ctactctggc ctatccaagg acttagccat ttcaactgcc gtaacgattt ctcgtcccta
    27541 ggcttatggt ttcgcctagc acaaacacta caggcatcac agcctgtgct agcatggact
    27601 ttcctcatga gaaacaattc tcctcacgcg attatccaaa aattgcacat ctcaaataac
    27661 tacttaaaaa tctgagttat ctaaaatttg tttaccaaaa acttcttttt ccaatctatt
    27721 caggcgtttc aaaatatcaa aattcgtcat agaacttgta attgccgctt caaggggttc
    27781 tgcttgaact ggtgaaggtt tcggtttacg agttaattct tccttcaaat ccgcaatttc
    27841 ctgacgaagt gacttgacca aggcagcata ggtttcatag tccttgatga catcgtctaa
    27901 aaactcgtca acttctactt tattatagcc acggacctca cgtccaaact cttgttcaaa
    27961 aatatctttc gctgaaaaaa taatacttgc catgtctctc tccattctcg cttgctagta
    28021 ttattatata aaaaaaggca aacaaaaatc aaggtcaaag cttcaatttt cggaaaaatt
    28081 ttcagcaagt tcatttaatt catcaaatgt taatctcttt ataaaatagt cctcttgatt
    28141 tttcatcttt tggtaaaaat aagctagttt tgtttcattt tcttcatcat aaaaaagata
    28201 ggaactagtc gtgttttcca gcaaaaactg ctggtaatct cttaactgcc ccttgtgttc
    28261 atagcgagga taggcatatt tgacaaagtc tacctgctta aagcgactca gtttcatctg
    28321 attgccttca ttccaatttt ccccatgggt ttcaaaagca aaaatggtgg ccaactggaa
    28381 accgtattca gttttcatct cctgagcaac ctctaaaacc caatattcaa aacccaaact
    28441 ccctgtaaac acaagccaag acaccccatc tgctgccata gcctctaaat ctttacgtat
    28501 cgctttttta atcaatttaa gacgaggatc cttatcagaa aataaaccca aatcaaaagc
    28561 agaatagcct aaaaccaaag ctgtagccat ttttaaccct ttctgtgcaa attatggtat
    28621 aataaagtga tgttattgta ccaataagga gaattatggt caactatcca cataaagttt
    28681 catcacaaaa aagacaaaca tctctttctc aacccaaaaa tttcgcaaat cgaggaatgt
    28741 cttttgaaaa gatgatcaat gctaccaacg actactattt gtctcagggc ttggctgtta
    28801 tacataagaa accaactcct attcaaatcg tacaagtgga ctatccacaa cgaagtcgtg
    28861 ccaagattgt tgaagcctat tttcgacaag cttcaacgac ggactattct ggcgtttata
    28921 atggatatta catcgacttt gaagtcaagg aaacaaaaca aaaacgtgcg attccgatga
    28981 aaaattttca tccacatcag attcagcata tggaacaagt ccttgcccaa caaggaatct
    29041 gctttgtcct tcttcacttt tcttctcagc aagaaaccta cttattgccg gcattcgatt
    29101 tgattcgctt ctatcatcaa gataagggac aaaaatcaat gccacttgaa tatattcgag
    29161 aatatggata tgaaatcaag gctggtgcct tccctcaaat tccttatctc aatgttatca
    29221 aagaacattt attaggtggt aaaacaagat gaacaaacca acgattctgc gcctaatcaa
    29281 gtatctgagc attagcttct taagcttggt tatcgcagcc attgtcttag gcggaggagt
    29341 ttttttctac tacgttagca aggctcctag cctatccgag agtaaactag ttgcaacaac
    29401 ttctagtaaa atctacgaca ataaaaatca actcattgct gacttgggtt ctgaacgccg
    29461 cgtcaatgcc caagctaatg atattcccac agatttggtt aaggcaatcg tttctatcga
    29521 agaccatcgc ttcttcgacc acagggggat tgataccatc cgtatcctgg gagctttctt
    29581 gcgcaatctg caaagcaatt ccctccaagg tggatcaact ctcacccaac agttgattaa
    29641 gttgacttac ttttcaacct cgacttccga ccagactatt tctcgtaagg ctcaggaagc
    29701 ttggttagcg attcagttag aacaaaaagc aaccaagcaa gaaatcttga cctactatat
    29761 aaataaggtc tacatgtcta atggcaacta tggaatgcag acagcagctc aaaactacta
    29821 tggtaaagac ctcaataatt taagtttacc tcagttagcc ttgctggctg gaatgcctca
    29881 ggcaccaaac caatatgacc cctattcaca tccagaagca gcccaagacc gccgaaactt
    29941 ggtcttatct gaaatgaaaa atcaaggtta catctctgct gaacagtatg agaaagcagt
    30001 caatacacca attactgatg gactacaaag tctcaaatca gcaagtaatt accctgctta
    30061 catggataat tacctcaagg aagtcatcaa tcaagttgaa gaagaaacag gatataacct
    30121 gctcacaact gggatggatg tctacacaaa tgtagaccaa gaagctcaaa aacatctgtg
    30181 ggatatttac aatacagacg aatacgttgc ctatccagac gatgaattgc aagtcgcttc
    30241 taccattgtt gatgtttcta acggtaaagt cattgcccag ctaggagcac gccatcagtc
    30301 aagtaatgtt tccttcggaa ttaaccaagc agtggaaaca aaccgcgact ggggatcaac
    30361 tatgaaaccg atcacagact atgctcctgc cttggaatac ggtgtctacg attcaactgc
    30421 tactatcgtt cacgatgagc cctataacta tcctgggaca gatacccctg tttataactg
    30481 ggataggggc tactttggca atatcacctt gcaatacgcc ctgcaacaat cgcgaaacgt
    30541 cccagccgtg gaaacgctaa acaaggtcgg actcaaccgt gccaagactt tcctaaatgg
    30601 tctcggaatc gactacccaa gtcttcacta ctcaaatgcc atttcaagta acacaaccga
    30661 gtcagacaaa aaatatggag caagtagtga aaagatggct gctgcttacg ctgcctttgc
    30721 aaatggtgga acttactata aaccaatgta tatccataaa gtcgtcttta gtgatgggag
    30781 tgaaaaagag ttctctaatg tcggaactcg tgccatgaag gaaacgacag cctatatgat
    30841 gaccgacatg atgaaaactg tcttagtata cggaatcgga cgtggagcct acctaccttg
    30901 gcttccacaa gcaggtaaaa caggtacttc taactatact gacgaagaaa ttgaaaagta
    30961 tatcaagaac actggttacg tagctccaga tgaaatgttt gtagggtata cccgcaaata
    31021 tgcaatggct gtatggacag gctattctaa ccgtctgaca ccacttgtag gcgatggcct
    31081 tacggtcgct gctaaagttt accgctctat gatgacctac ctgtctgaag gaagcaatcc
    31141 agaagattgg aatataccag aggggctcta cagaaatgga gaattcgtat ttaaaaatgg
    31201 tgctcgttct acgtggaact cacctgctcc acaacaaccc ccatcaactg aaagttcaag
    31261 ctcatcatca gatagttcaa cttcacagtc tagctcaacc actccaagca caaataatag
    31321 tacgactacc aatcctaaca ataatacgca acaatcaaat acaacccctg atcaacaaaa
    31381 tcagaatcct caaccagcac aaccataaca tttatcaccc agatttttct gggtgatttt
    31441 ttcatagcac tattgtattt atgtgcttaa caaaagcctg agaagacatc ttctcaggct
    31501 ttttctgtac tataaacctc attggaaaag atagagactg cttccgtaat cttgttaaga
    31561 ggttttacta aatactagtc tttcttcgtt tttactacaa agagagcaga tagggctagg
    31621 ctaacacctg ctaggaagag ggctgtgtca gattgactct cacctgttgc tggcaagatt
    31681 tgtttaggtg tttctggtct agctatttct tgcttataat ctggcatctt accttgatcg
    31741 gaagctaaag agctaagttt aaactcaggt ttctctacag ttggagcatc tggtttcgta
    31801 gcagaatctt ttaattggtt gagataatct gcaaagtatt ccaccattct ttccgtcaag
    31861 agatggttat ccgtcgcata tttcatgctt gactgaacgc tagctacttc ctcttgattg
    31921 tctttgtctg ttaatttctg agccttagcc aaagctgctt catagcctga caagtcaaga
    31981 ggtgtttctg ctacctgtgg acgagtaaag ataagttccg ctgcagattg gtatttatcg
    32041 ccaccatctc cgtaagtctt agtacctgta aggacaattt tcttagcctt aattgtctta
    32101 ccaaagtcaa tgtcttttgg cttattgtta tctggccaat cagttgcagt aaaggtatgc
    32161 tccttgccag actcatctgt cacaacaagt ttcacatcac gcaagttacc atttgaacct
    32221 gatccacgtg gaacataacg aagtccagtt atttcagttg gttctttcaa gaccatggtt
    32281 gcaggcttgc ctacatctcc tccgccccat gatgtatgcc ataaactaga taagtttcca
    32341 tcaaaggcat ttgcaagacc ttcttgagcc tgagcaggag ctgtaagact tgcaaagtca
    32401 tctgctacca aagccgtttt cttctgaacc aaagcattct tcaaggcttc aatcttggca
    32461 atctctgcac gcgcttcttc cacactgata tcatcatcgg cctgactgag gttaaagacc
    32521 gcctctttca aagcctccat agactctttg gtgtagttag tcatggcaac cgttggcaag
    32581 tagttcttca gagcattttc tgtcaacatc ttacctgtta gggtaatttc ttcgatttga
    32641 agattatcca tcatgaagtc gttataacca cggaagttgg catttccacc agaatcacca
    32701 cgagtattac ttgcatttcc agttgagtag atacctaccc aagtatcccc tgtttctgca
    32761 cctgtcacga ggaaggttgc cttcttggct ttcttagaat ctgtccaagt atttggcaat
    32821 tcatgcattt ccaagttgct tgcttgagta ccacgacgac ctgactggaa ttctccctta
    32881 ccgactacaa aagcataggt attgtctgaa cctgcttcgt attcaaaggt tacacggtag
    32941 gtcttacctg cttcaaaacg gaagttttgt gggatggttt ggtaaaccaa gttacgacgg
    33001 ctcactagtc catttgtctt gagtgaccaa tttccttcga taacatcatc gactttctta
    33061 ccattccaac cacgttgtgt atatggatcg tgtttttcag acaagtgagt gcggttgtct
    33121 tcgacacctt cgacaccacc cactacaaat gggaagatac cctgagcaac attttcaaag
    33181 tcttgcttga aggtgccttt acctgtatca tgcttgtctc cgtacatgct tgaattgttt
    33241 tcaaaggtac gaatttcatc aaagtaagtt gcttcatcac cagcttcacg actcaatgtc
    33301 agagtaacat ttgatacgtc cgatccagtt gtaaagaagg cgtacatgtt ttggaagtaa
    33361 cttgtatcgt caactgtagc attgttacga cgtgtattgt gggcataggc ttttacatag
    33421 ttgagggcga gagacttatt ggtataagta gtcacttctt tttcaccagt atttacagtg
    33481 atgctcgcct tggcattact acggttatcg acaccgacat aaacggcata cttggtattt
    33541 ggtttcaagc cagttaattt ctgagtgaga ctaacttttt ctttgtttcc ttgaatacga
    33601 agcatatcgt ttgccccttg agacttgaca atttctgcct tagaagcatc gcctgaaatg
    33661 gtccaatgtt tcaaggtacc actgttaaat ccttggtcat agatgtgcat gccttcactc
    33721 catgacattt caggattggt ttgtttcgaa cgatagagaa cgtatggttg atttgctaga
    33781 agatctaggg taattttacc atcttttaca gttagttctt gctcttctgt cttaccttgg
    33841 tcagttagct tgtaaaggta aaccttgctc tttgcccaat cgcttggaag ggtccaagtt
    33901 gttgcaccgg cctgcgtatt gaagtagtac atcttttcct tatcagtaga aagtttctta
    33961 ccatttgcat cccagttcca aggagtcaag taagctgaac catcttggat gacacgtccg
    34021 ttgagagtta ctgtacgttc gcgatattgt ggactattga catcatttga cttacgagtt
    34081 acaactactt tattattgtc agcatctacc aattccactc gcatttctgg agtccattta
    34141 taggtgctac cgttatcggt catagtcacc ggtgtaccat tttcccattt acttacagtg
    34201 aagtgttgga agtacttggt catgacgtca tgggcaaata agttagttac atagccattg
    34261 tagtcacttc ttccttgcca gccttcaaag tctttcatgc tgtagccacc tagcagtgga
    34321 tagttggctg caccaccata acttctgtag tcccctaccc aagcatcttt ttggtggtta
    34381 cgtataaagc gggtgatggc actgttgata cctttattgg tgtagccacc gtaggtcaag
    34441 tcagctgccc agtgatggaa ggtagagtcg tactcaccac catggcccca ctcgatcgca
    34501 aagcgccagc cttgtttgtt aatttcttta gcaagaacgt gggtagccca ggcaccgtta
    34561 tcacctgatt gaccattacc ccaaacgtcc acatagataa agtcgagacc gtcaccaagt
    34621 tttttcttca aatcttccca acgtgccaaa cgaccatgag ctaggtcata ggcagcatca
    34681 atgttgatac cttgatctag ccagttccaa ccatagctat agcttccatc tggattctta
    34741 cggagaattt tttcattgaa gtatttagac tcaggataag tttctgaagc gttaacgtgg
    34801 atacctagat gagctccata tttcttagcc ttctcaatta gggtcttgaa gtcttcgaca
    34861 ccaccgatac gcttaccaat atcagcatag ttcaagtgac cagagtcatg gccttcgcta
    34921 ccatatcctt taaggagaac accttgccca agaccatctg tgtggagatt gattttcttg
    34981 ataccatcca aggtcataag gaatgggttt tgtgcttgag aaccaaagtt catcgcgata
    35041 cggtaagctg tgatatcctt aactttttcc caaccttgag gattgttcat aatgctacga
    35101 taagcaatgg caccatcctg ccaatcgact ttcttgtctg cattggcatc ttcagtgata
    35161 acaaccttag cacttggaag ttccttcgtg tattctggga aaacaatgcc cttataagct
    35221 ttttcccatt gccattcaga gctgtggatt cctacatagt tggcatttcc gactgtttct
    35281 ttataggctg tcaaacgagt ccagtcattc gaaccaccac catagctgtt ttgagagtta
    35341 ctccaaacac cagcagcaag cttatctgta gaaacaaatc catacatgta acccttggct
    35401 aggtctttca ttggattggt tacatcgata tgatcatctc cgctgacatg cgtattgttt
    35461 gacatggttg ccccatcaaa cttagcacca gtttgatcac tagaaacaga gactaaagca
    35521 ttgccgagga aactaataga agaaagtagt tttctttcgt catcaatctt ttgacctgga
    35581 gtgacttgat tgtggttgac aatcttggtc acatcaaagt gcaattgatt gtccacaact
    35641 tgcaagcgta ctgtcatttc cgcattgatt aagtgagcat catcgcgaag cttcatcaag
    35701 tactctgctg ttgtctcatt gattttctta taagtgactt caggggtgat tcggtggtta
    35761 ttgataaaga cttggttgaa ctgttgaacc tgtcctggca aagtatgtcc attcaagctg
    35821 tattccttga cacgagggaa ggcttggtca atcactgctt tgagaacctt agactgaatc
    35881 gtgtcataag tcaccttgct atcatcaact tcaggacctg tttctttttc agcaggggta
    35941 tcctctgttt ttaccccctc ttggttatcc gttttaacgc taacaactgt tcgctcatcg
    36001 tcataagagc ccgccttgag aagaatcttc ttctcatttt taagatggtc attgaccgca
    36061 gctggtagag tcactgtgtc aaagagattg acatcgttat tgctggcatt tagctgaccg
    36121 tctgacttga gagtgataga gagacggttt gttgatcctg tttcaggagc agcaacacga
    36181 ctacctctat accaagtgct agctgttgga gatttatact cccagaacca gccatccttg
    36241 tcataaccga caaaaacatt attattggta tctttaaatt tcaagaagac accaaagcgt
    36301 gatttgccct tttcagaatc atctttgaag gttaaatcaa cagttgcatt tccattggca
    36361 tcaacggtca agcccttctt ttcaaacagg gctggtttac ctgcgttatc attttgagca
    36421 gttgaggata attggttgta gcggacacct ttttcttctc ggatagtgac tgttccctgt
    36481 tgttcttttt tctctaccgt ttgccattca ggggttaccg tcttaggtgt ttcaggttta
    36541 gcagctgcag gtttatcctc ttttggtttt tcttcagccg gtttttcttt ttcaagtgct
    36601 aatagttctt cttctgtctt aggtccatct gtaacttctg gagaaccttg gtcttctccc
    36661 accttaggcg cttcaaaatc acgcccatca ttctcttttg ctgttgcaag agcagtagct
    36721 aaatcagctg gtaagttcgc cgtggaacca gactgcacgc tatctgcaag aaccggactt
    36781 gtcccaaaga atgcagctcc aatcataaca gaagcaacac ctaatgaaaa tttccgaata
    36841 ctatatttac aacgtttttc aaataatcct ttattcataa acttctaaat cctttcattt
    36901 ttgagcaagc gtttacattt cctttttaaa aaatactcct tcctctgaaa gtgcaatgct
    36961 taaccctgat aaataaaaaa taattatact tttaatataa caaataatag ctgagaaagc
    37021 aataggaaac aagtaaaatt aacaaaaatt ttctctattc tgacaaaaag aggctccaac
    37081 ttttgggggt caatacattt agaatctttc tttacaaatg taaaaaagga ttcagcattc
    37141 aagagaaata ctaaatcctt tcttatattt tgcaacagtt atttcacatg ttttgcgaga
    37201 tcttcttgag cctttttatt agactcttct ttttctttca tccatttttc ctgagctttt
    37261 tggtattcat ctacagtgac tgccttgtct tgaagttcca agtatttata taagactggt
    37321 tcacttgtac ctttatttcc tgacaatgca aatggtattg taaatggtac catcttagac
    37381 aagattggac gccctgtacg agatgtagtt ggaataatca aagcactatc tgtcaaccaa
    37441 gcttgggctg cagcgtattt atcatagcgt ttagcaacat ctgtagcctc atcaccagcc
    37501 tcagtaacca atttttcgta gtcatataga cctacttttt tagcagctac attatcttcc
    37561 cctgagtcaa accctaaata tgttttagta ctttctccta cagaaggttt gataatatca
    37621 aggtaggttg atggatcggc aaagtctgga ccccaaccga cattatctga taaatcccag
    37681 tcttcgccag cagcattttc agcaaaatat gtaatattgt ttacttcgtc tttttgtagt
    37741 tgttgaatat caataatgac attatcagct cctaaagttg cttccaagga ttgtttcata
    37801 gattggacgc gctgaacttt tgtagttgct gtttggtcaa ctggcatatc caaatgaatt
    37861 gggaatgtca caccttctgc ttgtaaggct gatttagctt tagcaaattc agccttggct
    37921 ttttctggat tgtaaagacc atcctgagaa tctgcaagat taacatcctt ccattcatcc
    37981 ccataagtga ccaatttctc tttgaccata tcgccaaagt ttttaccatc tgcttgaaca
    38041 aatgttggtg gcacaaagag attacgcaag attttacttg ctccagtttg tccattcaac
    38101 tgagaggcat aggctgtacg gtcaaatcca aatgcaatag cctgacggaa atccttgttt
    38161 aagagagcct ttttagtcga tgccttttgt tcgtcgctgg tcttagatgt gtatttatag
    38221 gactgacggt caatatttgt accaactaga tacgtaatag agtcttgttg agtatagaca
    38281 atattgtcct tcatactctt ctcaagctct gcgaaacttg cacttgttgg atagagacga
    38341 gctgctgtaa ggctaccatc tttaaagttt tctgcaggtt tgctggtatc ttgaccatcc
    38401 cagaatgaca atttaacttt gtcaatatgc acattgtcct tatcccagta gttcggattt
    38461 ttcgcaaatt caacagagga tttggtcaca atggatttca acaaataagg accgttatac
    38521 aagagactac ttggatccgt agctttggca aaatcatctc cttttgaatt caaaaactct
    38581 tcattaactg gcgcaagcac acccatggtt gtcttagaat tccagaagct ttctggtttg
    38641 ttcaaagtgt actgaactgt ctgttcatcc agagccttaa ttcctacttg tgagaaatct
    38701 ttgatttccc cttttacata ggcatccaac cctttgattg attcttgaac aaggtaaaga
    38761 gcatctgatt ttttatcagc agcatatttt agtcctgtta caaagtcttg agctttgact
    38821 gccgcgtatt cttcaccttc agaagtatac cattttgcat ccttacggat agtataagtg
    38881 taagtcaatc catccttgga tacagaccaa tcctcagcca tagacggcac aaagttcccg
    38941 tagcgatcat tttctagcaa accatcaacc acgttactgg taatatttgc tgtcgcagcc
    39001 ttagcagttg tcaaatagtt gaggttatca gggtctgtct cataaatgta tgagaatgtc
    39061 ttctcacctt tagcgcttga acctgatcca gagcatgcag ctaaagtagt cgccgccaat
    39121 aatgtcacgc ccgcaagggc aagtagtttt gaacttttca tcattttctc ctttaaaact
    39181 ttctctccat tatagactct tttcagaaag ttgtcaacag aattttcaga atttttgaaa
    39241 attatttttc aaacaacatc tttgcaaaaa atatgaatat catttaaaaa tccctcaact
    39301 atatcacata gtcaaggaat ttttaggttc tataatattt gtagtgggta aatccactat
    39361 agatattatg gagcctattt tt
//
LOCUS       DAVHEX010000011        37975 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVHEX010000011 DAVHEX010000000
VERSION     DAVHEX010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37975
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.011"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            101..283
                     /locus_tag="VVL45_000577"
     CDS             101..283
                     /locus_tag="VVL45_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4186792.1"
                     /translation="MDLKFEGVDLEYKKAKNNLPESFWETYSAFANTNGGKIILGIDE
                     KNIDTYQRVNRLPAKL"
     gene            228..1041
                     /locus_tag="VVL45_000578"
     CDS             join(228..605,607..1041)
                     /locus_tag="VVL45_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEU4186793.1"
                     /translation="MKKTLTPTKESIDSLRNYEASKQLTDTRFKRLVGVQRTTFKEML
                     AVLKTAYQLKHAKGGRKPKLSLEYRTYEEIAADFGIHESNLIRRSQWVEVTLVQSGVT
                     ISRTPLSSEDTVMIDATEVKINRPKKELANDSGKKKFHAMKAQAIVTSQGRIVSLDIT
                     VNYCHDMKLFKMSCRNIGQAGKILADSGYQGLMKICPQAQTSRKSSKLKPLTAEDKAY
                     NHALSKERSKVENIFAKVKTFKMFSTTYRNHRKRFGLRMNLIAGIINHELGF"
     gene            complement(1093..1455)
                     /locus_tag="VVL45_000579"
     CDS             complement(1093..1455)
                     /locus_tag="VVL45_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4186794.1"
                     /translation="MPKTKTLAELADVILWSFDFANDHAHAFFMDNVEWSHADSYFRS
                     FVSDDVEERYTENVYLDSLSVKQKFKFIFDFGDEWRFECQVLREIETEDEEAYLVRSV
                     GTSPEQYPDYDGFDYEEW"
     gene            complement(1456..1665)
                     /locus_tag="VVL45_000580"
     CDS             complement(1456..1665)
                     /locus_tag="VVL45_000580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4186795.1"
                     /translation="MKQKNYLVSNATVRQTYDKIAESECFLRAIGGIMLHLKLVKQEI
                     EAEKPASVEAWIISVKFKKGCYRHI"
     gene            complement(1817..3697)
                     /locus_tag="VVL45_000581"
     CDS             complement(1817..3697)
                     /locus_tag="VVL45_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HEU4186796.1"
                     /translation="MVRFTGLSLKQTQAIEVLKGHISLPDVEVAVTQSDQASISIEGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEILALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDVTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDGLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDIL
                     CPILDQHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(3691..4560)
                     /locus_tag="VVL45_000582"
     CDS             complement(3691..4560)
                     /locus_tag="VVL45_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU4186797.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNILCQEAIERMNRNLAQGLLN
                     IQYLIDPGVISLGGSISQNPDFIQGVKKAAEDFVDAYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(4643..7288)
                     /locus_tag="VVL45_000583"
     CDS             complement(4643..7288)
                     /locus_tag="VVL45_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEU4186798.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGKQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKDHEVLENTDRYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELTVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFALECHQAQERFSAYRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVLRYYNMCSENVRVPESQHLFLDLLE
                     RPYPVHSGLLAPQEIRTEFIKKEEI"
     gene            complement(7379..8659)
                     /locus_tag="VVL45_000584"
     CDS             complement(7379..8659)
                     /locus_tag="VVL45_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEU4186799.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEKNWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETGQFDETF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFVELDLADSQSVIADAKRLQDEIQEGIENYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSVDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            8830..10914
                     /locus_tag="VVL45_000585"
     CDS             8830..10914
                     /locus_tag="VVL45_000585"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEU4186800.1"
                     /translation="MKPLLETIDTRFGTTNKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTGQLGGDSLFHRQSSYDRDK
                     ASFQPHYLKIFSLRYQIETQLTPTCYGASIRLKQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEGGDWRIDLVSSQTEMQLATSFIS
                     PSQALINLPQEDFDSCKASAQADWENLLHRFDVIETGEADRTFFDHCLYRLFLFPQTF
                     YEVDESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKENKWLDIHTKQNHNHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPNH"
     gene            complement(10954..12633)
                     /locus_tag="VVL45_000586"
     CDS             complement(10954..12633)
                     /locus_tag="VVL45_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEU4186801.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGDCLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNLDKLGTEAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFDCRHLTDGLETSSWASDADLPIQLELDLGSPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGRGFTVGHKRLLRGPLVEAQKVRVMITEAQSIPVLTKISLYKT
                     PGLSKKEVVQELAFAEKSLAVAKGETLHFRIERSESSSSLEAKISIQPGTGVHGVAYQ
                     DEIYVLAFQAGETEKRLTLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
     gene            13389..14618
                     /gene="arcA"
                     /locus_tag="VVL45_000587"
     CDS             13389..14618
                     /gene="arcA"
                     /locus_tag="VVL45_000587"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEU4186802.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESEYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVISKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            14676..15692
                     /gene="argF"
                     /locus_tag="VVL45_000588"
     CDS             14676..15692
                     /gene="argF"
                     /locus_tag="VVL45_000588"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEU4186803.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            15857..16804
                     /gene="arcC"
                     /locus_tag="VVL45_000589"
     CDS             15857..16804
                     /gene="arcC"
                     /locus_tag="VVL45_000589"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEU4186804.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            17013..18524
                     /locus_tag="VVL45_000590"
     CDS             17013..18524
                     /locus_tag="VVL45_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEU4186805.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYEA
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            18546..19877
                     /locus_tag="VVL45_000591"
     CDS             18546..19877
                     /locus_tag="VVL45_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEU4186806.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     LLYEQVCNGIKEAGYDYQTTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVKLLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPEAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            19971..20098
                     /locus_tag="VVL45_000592"
                     /pseudo
     CDS             19971..20098
                     /locus_tag="VVL45_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836165.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
     gene            complement(20281..20571)
                     /locus_tag="VVL45_000593"
     CDS             complement(20281..20571)
                     /locus_tag="VVL45_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4186807.1"
                     /translation="MGALGYYEGFVPYVSNQYKNQTEEEGKPLSDKYIFEKILGKTYA
                     AFKKDQINERVEKLGKLKPITINYNGKSEVIDSKEKLQKLMNKAVKDEVAQI"
     gene            complement(20559..20810)
                     /locus_tag="VVL45_000594"
     CDS             complement(20559..20810)
                     /locus_tag="VVL45_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEU4186808.1"
                     /translation="MKEFQFERKQRFSLRKYAIGACSVLLGTSLFFAGMGAQPVQATE
                     TSSALISSHYLDEQDLSEKLKSELQWFELENKLLNLWEH"
     gene            21014..21172
                     /locus_tag="VVL45_000595"
     CDS             21014..21172
                     /locus_tag="VVL45_000595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4186809.1"
                     /translation="MKIKEQTRKLATGCSKHRFEVVDKTDEVSSKHCFEVADRTDEVS
                     NIYTARRR"
     gene            complement(21209..22093)
                     /locus_tag="VVL45_000596"
     CDS             complement(21209..22093)
                     /locus_tag="VVL45_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU4186810.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            complement(22238..23389)
                     /locus_tag="VVL45_000597"
     CDS             complement(22238..23389)
                     /locus_tag="VVL45_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEU4186811.1"
                     /translation="MATFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKTLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGDMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            complement(23535..25301)
                     /locus_tag="VVL45_000598"
     CDS             complement(23535..25301)
                     /locus_tag="VVL45_000598"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEU4186812.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEVSDTDIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRS
                     IYDPEEFERALKWVKENVKEGFDHNREDLVLSREEKDRQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTGHTLEGRAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYCACQLLGPLHK"
     gene            complement(25384..28500)
                     /locus_tag="VVL45_000599"
     CDS             complement(25384..28500)
                     /locus_tag="VVL45_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEU4186813.1"
                     /translation="MNKEKIKRKLITILFVCIGMLCFGLLAGVKADNRVQMRTTINNE
                     SPLLLSPLYGNDNGNGLWWGNTLKGAWEAIPEDVKPYAAIELHPAKVCKPTSCIPRDT
                     KELREWYVKMLEEAQSLNIPVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENY
                     WIYNNQLAPHSAKYLEVCAKYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLA
                     TKNTPIRDDAGTDSIVSGFWLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYA
                     SEPESMIAMEMMNVYTGGGTVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAP
                     SKEEVVNRTKAVFWNGEGRISSLNGFYQGLYSNDETMPLYNNGRYHILPVIHEKIDKE
                     KISSIFPNAKILTKNSEELSSKVNYLNSLYPKLYEGDGYAQRVGNSWYIYNSNANINK
                     NQQVMLPMYTNNTKSLSLDLTPHTYAVVKENPNNLHILLNNYRTDKTAMWALSGNFDA
                     SKSWKKEELELANWISKNYSINPVDNDFRTTTLTLKGHTGHKPQINISGDKNHYTYTE
                     NWDENTHVYTITVNHNGMVEMSINTEGTGPVSFPTPDKFNDGNLNIAYAKPTTQSSVD
                     YNGDPNRAVDGNRNGNFNSGSVTHTRADNPSWWEVDLKKMDKVGLVKIYNRTDAETQR
                     LSNFDVILYDNNRNEVAKKHVNNLSGESVSLDFKEKGARYIKVKLLTSGVPLSLAEVE
                     VFRESDGKQSEEDIDKITEDKVVSTNKVATQSSTNYEGVAALAVDGNKDGDYGHHSVT
                     HTKADSNAWWQVDLGEEFTVSKVDIYNRTDAEPQRLSNFDVIFLSSSGEEVFRRHFDK
                     VVDGLLSLKVPSVGAKLVKIELKSAAIPLSLAEVEVYGSKRTPKKLSNIALTKETRQS
                     STDYNGFSRLAVDGNKNGDYGHHSVTHTKEDSPSWWEIDLAQTEELEKLIIYNRTDAE
                     IQRLSNFDIIIYDSNDYEVFTQHIDSLESNNLSIDLKGLKGKKVRISLRSAGIPLSLA
                     EVEVYTYK"
     gene            complement(28510..30804)
                     /locus_tag="VVL45_000600"
     CDS             complement(28510..30804)
                     /locus_tag="VVL45_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEU4186814.1"
                     /translation="MKLWYKKAAANWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTVFATPRDQSHYELLGELYIE
                     HIDIQSCALSLYERELDLDTAISNVVFEPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTDYWGNIDISSLQGEFSSIDYFTEKDEHVKKYQEQFNRVD
                     FKLDYSKGCLSIPTNLLLENTKKYSNYLTNLLFHYGRYLLISSSQPNGLPANLQGIWC
                     DELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLERMREPGRLTAKKMYGAR
                     GFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHYLYFQDERILTEHFEMIK
                     EAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACLSSTIDNQILRYFCDSCI
                     GIAKQLGDNSDFISRVKELKKKLPKTKIGSNGQIQEWLEDYEEVEPGHRHISPLFGLY
                     PYNEIDIHKTPELAEAAKITINRRLSNANFLSSQEREQAINNWLVSGLHASTQTGWSA
                     AWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLGLVSGICELLVQS
                     HHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDITFLKLEGGNKDQKVRVRI
                     YGKNTDVQNIELVFNSEKIIELNF"
     gene            complement(30863..31663)
                     /locus_tag="VVL45_000601"
     CDS             complement(30863..31663)
                     /locus_tag="VVL45_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU4186815.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            complement(31660..32433)
                     /locus_tag="VVL45_000602"
     CDS             complement(31660..32433)
                     /locus_tag="VVL45_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU4186816.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            complement(32458..32928)
                     /locus_tag="VVL45_000603"
     CDS             complement(32458..32928)
                     /locus_tag="VVL45_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU4186817.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            complement(32919..33350)
                     /locus_tag="VVL45_000604"
     CDS             complement(32919..33350)
                     /locus_tag="VVL45_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU4186818.1"
                     /translation="MFNRGILVASHGNFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVDELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISSYDSKINLEEIVHNAQNSLFNVKQQLNVEEEEDLCL"
     gene            complement(33340..33783)
                     /locus_tag="VVL45_000605"
     CDS             complement(33340..33783)
                     /locus_tag="VVL45_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HEU4186819.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENVQ"
     gene            complement(33795..34433)
                     /locus_tag="VVL45_000606"
     CDS             complement(33795..34433)
                     /locus_tag="VVL45_000606"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HEU4186820.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMELMAEKFKTYGQRK"
     gene            complement(34548..35951)
                     /locus_tag="VVL45_000607"
     CDS             complement(34548..35951)
                     /locus_tag="VVL45_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEU4186821.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLLQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKEE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPILTTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGLTEATAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            36127..36900
                     /locus_tag="VVL45_000608"
     CDS             36127..36900
                     /locus_tag="VVL45_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU4186822.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCIYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTVPDQKLLDAVELFDGEWVSDIQ"
BASE COUNT        11256 a         8276 c         6804 g        11639 t
ORIGIN      
        1 agaaattaac tgagtaaaaa ctctcgaaat tcaattcgct atattatcac tcaacttgaa
       61 caaataccag aaccacaaaa aacagatgta aaggaaaaat atggacttaa aatttgaagg
      121 ggttgattta gaatacaaaa aagctaaaaa caatcttcca gagtctttct gggaaaccta
      181 ctcagcattt gcaaatacaa atggcgggaa aataattctc gggatagatg aaaaaaacat
      241 tgacacctac caaagagtca atagactccc tgcgaaatta tgaagcaagt aaacaattaa
      301 ctgatacacg atttaagcgt cttgttggtg ttcagcgcac gacttttaaa gagatgttag
      361 ctgtattaaa aacagcttat caacttaaac acgcaaaagg tggacgaaaa cctaaattaa
      421 gcctagaata ccgcacttat gaagaaattg cggctgattt tggtattcac gaaagcaact
      481 taatccgtcg gagccaatgg gttgaagtaa ctcttgttca aagtggtgtt acgatttcaa
      541 gaactcctct tagttctgag gacacggtaa tgattgatgc gacggaagta aaaatcaatc
      601 gccctaaaaa aagaattagc gaatgattct ggtaaaaaga aatttcacgc tatgaaggct
      661 caagcgattg tcacaagtca agggagaatt gtttctttgg atatcactgt gaactattgt
      721 catgatatga agttgttcaa aatgagttgc agaaatatcg gacaagctgg taaaatcttg
      781 gctgacagtg gttatcaagg gctcatgaag atatgtcctc aagcacaaac ttcacgtaaa
      841 tccagcaaac tcaagccact aacagctgaa gataaagcct ataaccatgc gctatctaag
      901 gagagaagca aggttgagaa catctttgcc aaagtaaaaa cgtttaaaat gttttcaaca
      961 acctatcgaa atcatcgtaa acgcttcgga ttacgaatga atttgattgc tggtattatc
     1021 aatcatgaac taggattcta gttttgcagg aagtctattg aaatactaag cctacacaga
     1081 ctgatttcaa ttttaccatt cttcatagtc aaaaccatca taatctggat attgttctgg
     1141 cgacgttcca accgaacgta cgagataagc ttcttcgtcc tctgtctcga tttctctcag
     1201 cacttggcat tcaaaacgcc attcatcacc gaagtcgaaa ataaacttaa atttttgttt
     1261 gacacttagg ctatccagat agacattttc tgtgtaacgt tcttcaacat cgtcactaac
     1321 aaagctacga aagtaagaat ctgcatgact ccactcaaca ttatccatga aaaatgcgtg
     1381 agcatgatca tttgcaaaat caaaactcca taaaatcaca tctgctagtt ccgctaacgt
     1441 ttttgttttt ggaatctata tatgtcggta gcaacctttt ttaaatttga cggaaatgat
     1501 ccaagcttct acagatgctg gcttttcagc ttctatttct tgttttacta attttaagtg
     1561 tagcataata cctccaattg ctcttaggaa acattcactt tctgctattt tatcataagt
     1621 ttgtctaact gtagcatttg aaaccagata attcttctgt ttcacgcgaa acaccctgcg
     1681 ccaagcaagg tgttcttgtt tttatactca atgaaaatca aagagcaaac taggaagcta
     1741 gctgcaggtt gctcaaaaca ctgttttgag gttgcagatg gaagctgacg tggtttgaag
     1801 agattttcga agaatattaa gtcgtataaa tcgtcgacgc tgtcgcaatg gtatgccact
     1861 ggttggctgt agttgctgcg aaatccttgt ctgcgtagaa gtcagtaaat ggtaggattt
     1921 caacttccag ctcgtcgatg cggtcaagct gaccagccag ataaacctcg atacggcttt
     1981 ctgctcgttt gatgcgttgc aagagtccgc ccatacggat gtcaactgta tccaaaccaa
     2041 agaccttgtt ttctttcagc cattggtggc taaagagggc atggaagtct tcaatttggc
     2101 ttctaagttc tggtaattct tgtctggcga tttgttgtaa actttcttta tcatccgctt
     2161 ggtaggcctg acgaatgcgt cgtcccacat ctactttgct acttaaaata gcattcaact
     2221 gggcctgagt ttcaaagaga taggcatagt ttccagcttt ttctttaatg ttagcaagcg
     2281 tctcagcagc ctgagcgaag tgcggtttgt cctgttcagg tgtcatgtgt tgatcaagaa
     2341 tcggacaaag aatatcctga taaaaaacat agcggttggg attgataccg ctgagattgc
     2401 ctggtaggtc tggtaagagg ttggcaaggt caatctgcat aaaatcctca accgttagac
     2461 cagtattggt cttgaagtgc gcagacaaac catctaggtc attgcgatag ctgagttctg
     2521 cccagatttg caagcttggt aggatagaga actgggcagt ttcaccacca ttgtctcccc
     2581 aacccgttac gatgacttct ttaatctgat tggcacggca ggctttatta gcctcgatag
     2641 ccactagacg gctaaaatgg ttgtgaggtg taaagccaat ccacttccaa gctccccctg
     2701 caaatgcaag gtcatggcta atcttgtgat gattgcggaa attacggttg tatttttcct
     2761 cgctatcctg ataataatcc cagtaaacca gagtcacacg gtctttgaga cggtctaggt
     2821 agacacgagt ttcctctgga atttccacat cacggtcgta ctggccatcc gctgacatga
     2881 gtttgaagaa catatcactc cacatctggc agtggaaacc atatttgtca gcaatatcca
     2941 gcacgcgctc caagtgttgg cacatgagga gactacgatc cacaacaccg ttcagaatca
     3001 ggtagcgtcc caaaccaacc aagtgggctt cgtccatccc gatattgacc ttgcgagtct
     3061 tcagtttaga caacgtggca aacatgccat caatcaagtc ataaaccttt tcttcgccaa
     3121 tgagaagaat gtcctctaca tcacggagct cctgcacttc cttgacaccc catttgacaa
     3181 aggccgacaa gtgggccaag gtctggatgc atggtacaaa ggtcacgtca aactgttggg
     3241 cataggcttc gatttcctgc aactcctctg ctgaataagc tccacggaaa tagccaaagt
     3301 aaggctgccc ttcaatctgg taagtgtctt ccatgtaaag ctcaaaggtt gagtagccca
     3361 tgagagccaa tatctcaatc atctgcttgg cagaagccac attcagcacc gcatttcgag
     3421 aacagtcaac catgtaagcc aaatcttcgt aagctgcttg ttcctcaatc tctactttat
     3481 cagcttctgc tagaactgtt accaacaagg acaaggcacg ataaagttgg tgaggtttgc
     3541 ggtaggtcaa ttgatagtga ccttcctcac cctcgataga gatagatgct tggtcagact
     3601 gagtgacagc cacttccaca tctggtagag aaatgtgacc ttttaaaacc tcaatagctt
     3661 gcgtttgttt gagactaagt cctgtaaatc ttaccattgc ttttcctcct gtagccagtt
     3721 gacaagagca ccgtagagat tggcatctgc gtgataggtg caggcctgga taactggtgc
     3781 gaccgtgtat tcttcgtagg catcgacaaa gtcttcagca gccttcttga caccttggat
     3841 aaaatctgga ttttgactga tagagccacc cagactgatg acacctggat cgatcagata
     3901 ctggatattg agcaagcctt gcgccagatt gcggttcatg cgctcaatgg cttcttgaca
     3961 aaggatatta ccagctgcgg cctcttggta aatcttgcga ccgtcccaat cagtatgacc
     4021 agatttttca atcacgtatc gtaccatatt cccagttgat gctagttgcg accagttatt
     4081 aagtttttca gcaggggcaa gggttgtcat gtagccaaat tctccaccca gaccgtggcg
     4141 acctcgatga agtctaccat tgataatcat ggctccgcca atccctgtcc caatcacgac
     4201 acaggctgca ttttcaagct ctggatgagc tagtagttca ctgagtccaa cgcagttggc
     4261 atcattttct aaatggacag gtagctgata agagctaagc gcctcatacc aagaaaagcc
     4321 atggatgtag ggcaccgcac tgaagccatc aatcacacct gtctcttgat tgactgcacc
     4381 tggaacgctc atagcaatcc cactgtaatc ctgttctgac aagcgttgat ctagccacgc
     4441 tagtaaatcc tccaagtttt caggcgttga aatacttgtc ttatccagta ttttcccatc
     4501 aggagtcaga ctggcaaact taatcccagt ccctccgata tcaatcgttg caatggtcat
     4561 tgtttttacc ataaaagggg cgaatcagca gttagttctt actttttcgc ccctcctttc
     4621 ttttgatgtt tactttttga aattaaattt cttctttttt gatgaattct gtacgaatct
     4681 cttgtggagc caatagtcct gaatgaactg ggtatggtcg ttcaagtagg tcaaggaaga
     4741 gatgttgact ttctggcaca cgtacatttt cactacacat attgtagtaa cgaagcacat
     4801 agccttcttc attttcagct accttaaagg ctgttggaca aacttgcggt atgctgagaa
     4861 cagaatggct caagaggcta ccagtcgcaa ccacgcttcc ttcctgtcta gcaagctgaa
     4921 ggctggtaaa cggtgtctgc aaggctttgg cacgacgata ggctgagaag cgttcttggg
     4981 cttggtggca ttcaagtgca aactcgactt caaactcccg caagcattgt gcttctggcg
     5041 ttgggaagta gccccagtca cctagctcac ctgatgcacg caaaatggtc acggcaatgg
     5101 tgttatcccc aaggatttcg tattcattca atcccttgtt ggatacagtc accccttttt
     5161 catcgtcata cagactgaca aaagcttgtt ggtgttgagg attttcaggg ttttcccatg
     5221 aagcagctgg tttgtttggt cgtgtcacca cctcatagat actttcagaa tcattgcttg
     5281 gacgcgtgtt atgagtcttg accaagagac ggatacggtg atccttggca gtgttagtaa
     5341 agcgagtctt gaagcggatt tgtggattgt caacgaagac agtcaactca gtttccagag
     5401 gaatgtttgt caattcttct gaccgtccag cctcacgctt cataaactcg atgatacctt
     5461 gttgctcttc ttctagcttt tcatccgcac tgacaggcac ggtcaattca tgtttgagca
     5521 agattttagc atagcgatct gtgttttcca agacctcgtg gtccttaagc tctgcaaaga
     5581 ttggctctgt tccttttggt tggaaataga tatactcgtt tccgatgtca ccccggtctt
     5641 caaagcggat aaagtcttca taggcttcgt gagttgtctt gtcatagact gtgatgttgt
     5701 catccacact caccgttacg aatggtgtat caatcactcc gttttggtaa ataccctcac
     5761 ggtgttcttg ttttccttcc agcaattgga aggttgtcca agaaagcggc gctaggtgaa
     5821 ctggaatggt cacgcgcact tgtcgagcaa tacgagcttg gcggaactta tcttttggta
     5881 aatcgtactc aaaattagct ccgaggtctt cgattgtagc ctctacagga cgaccatcca
     5941 agtcctccac acggtaactt ggcaagataa gagcagccat ctttttgtag ccttctgttg
     6001 ggtgcaattc cttgaaatca caagtcgcca catcaatcac tgtgctgaca gtatcgacct
     6061 tatcatgcaa gcctgtgtta atgacagtaa agagatagtc actttgagcc ttatccgtag
     6121 caattttacc cttccactcg ttgagcaagt tacttttaac aaagtttcct acttggttga
     6181 ccttggcaaa acgcgtttcc atctcgcggt gaacttcgtc cacgctacag ccacagatac
     6241 tatcatgtgg cgcgttctgc aaaagtgttt tccaagcata ggtcaactgg tccttgtggt
     6301 tgtgtccacc agtgataata gtcaagggtt ctaccacttg ctctaggagg ttgctatttt
     6361 cttggaaggc ttgttttagg taaatgcggg atgaagaagt gttggcaagt gtgtaccagc
     6421 catctgtttc ctgactggtc aactcgcctg taacagttga taagtgttca ggaagcgcac
     6481 cttctacagc ttgaacatat tcatcaaaag aactatgaac aaagattaca tccgggaaga
     6541 gttcatttgc cacacgaatg gcttcgctca gatttttctg tacaggctgg tggtcacagc
     6601 cgttcatcat caaccattgg ttggtcgaag cgtaggcacg cacatctgac aatttttgtt
     6661 tccagaaggt caaggcctca tctttgtcaa ctgggatttc attcccatta ctgtaccagt
     6721 tggcaaagag aatacctaaa acacgactac catccacacc ctgccagtac atttctgaaa
     6781 actgggatgt aaactgctca tcttcaagga cttggttgtc aaatccaatc ggcttcacac
     6841 cacgaccaaa ggccgccaca tgaatgcctg atttttgaag aatttgaggt gcttgtccca
     6901 tatttccaaa ggtatctgga aagtagccaa tctgggttga tttaccccat ttggcagctt
     6961 cttgttgacc aatcaaggta ttgcggacat tggcttcact tgaaatcaaa taatcatcct
     7021 gcaagatgta aaagggacca attttaagct tgccctcgtc aatgtagcgt tggaccttgt
     7081 cgcgattttc agggcgaatt tgtaagtagt cgtcaaggac aatagtttgt ccatccaagt
     7141 ggaaactctt gaactcaggg tcattttcaa agagatcaaa gagattgtca aacaattcca
     7201 ccaactgcat acgatgactt tcaaaaggca agtaccactc acggtcccag tgactatgtg
     7261 agataatatg tacaacaaca ttttccatga agtaaaacct cattctaaat ttaaatttta
     7321 acgttttaaa tgtaaacttg tgattctgat aagaatcggt tgagctaaag cgagcccctt
     7381 agcgaatatc caagtaatcc aagaccaact cacagaacat catgttagcc caggagaacc
     7441 attcacgaga gtagagagtt gggtcatcca cgtggaagct ttcgtgcatg acacctgtac
     7501 caccatcgca ggcaaccagc tgatccagca agaatttttt ctctgcctta tctcttgttg
     7561 tcaatccttg gatagaaagg gcgattggcc agatatagcg atagaaggta tgagaacttc
     7621 cgagaccgct agcgtattct ccttggtaga aatatggatt ttcagagctc aaaatggtac
     7681 gacgagtagc ttgatacact tcatcatcga ccgaacagta gcccagatag ggcgcagcta
     7741 gtaaacttgg tacatttgga tcatccatga tgctggcatt tcctaggcca tctacttcaa
     7801 aagcgtaaat cttttcgccc ttgctgttgg tggtgtaagc gtagttttcg attccttctt
     7861 ggatttcatc ctgaagacgc ttggcatcag caataacact ctggctatca gctaggtcta
     7921 attcgacaaa gatttcttgc acataaccca agactactac ggcaaacata ttagacggaa
     7981 tcaagtaact atactgacag caatcgtcac tcggacgaaa agctgaccaa gtcatacctg
     8041 tcactgcaaa gtcaggtcca aagccatcat ttaccaaggt gtcttcctta cggtccgtat
     8101 ctcggacaaa acgataagga gagttcttgt ggtcttgttc aactgtccat agatggagaa
     8161 tttccttagt cgctgcgaca aaagtctcat caaactgacc agtctcgcca gtctctttcc
     8221 agaggagata agccaactgc aaaggatagc aaagtgaatc cacctcatac ttgcgctccc
     8281 agatccagcc gttaaggtct gtgtggtcag tctcgtggtg ccctttccag ttcttctcaa
     8341 tgttgaagga gttagcatag ggatccttga gtaccaaggt catctgacgt ttgaccaaac
     8401 ctgcaatggt ctgacgcagg agggcatctc ttttagctac atgaaggtag ggtctgagtt
     8461 gggctgtcga atctcgaagc cacatggcag gaatatcccc agtcaagaca aaagttgaac
     8521 catcttctaa gatttcaacc gtattgtcca aggtatcggt gtagcaacgc tcgaaaacat
     8581 ccacccactc tggatggtcc ttagcccgct ctgctacttc atctagccat tctctaacaa
     8641 tttctttcga ataaatcatc gtgcaattcc ctctttcaaa caagtttcat gttcagtata
     8701 tagcttttga gacagaaaat acatacacaa atgatagtca tttttctaca aaatagttat
     8761 ttttgaaact ccttttctaa aggccctctt tttctgatat aatgtagaaa aacagctaaa
     8821 ggaaagacta tgaaaccact acttgaaacc atcgataccc gctttggaac taccaacaag
     8881 catgcctttt ctcggggaaa taccctgcca tacacaggcg tgccttttgg gatgaattac
     8941 tttgtgcccc agaccagtga ccaggatgga tcttggtttt ttgatccgca tctgcctatc
     9001 tttcagggga ttcgattaac tcaccagccc agtccttgga ttggggacta ctcttggctc
     9061 cttctgacac ctgtcacagg tcagcttggg ggagacagtc tctttcaccg ccaatcttcc
     9121 tatgataggg ataaggcctc tttccaacct cattatctaa agattttctc cctgcgctat
     9181 cagattgaaa cccagctcac accgacttgc tacggtgctt ctattcgttt gaagcaaaag
     9241 caaggcaaag ccctctccct ctatcttcac gcagcagatg aactgacagt agagcaagta
     9301 gataagcgaa ctcttgccct gcgacaagaa ggtaaaactg aaaccaacaa aaattcgcta
     9361 acgatgttca ctgccctaca aatgaacacg gatattcttg ctatcagcca agaaggggga
     9421 gactggcgaa ttgacttagt aagtagtcaa accgagatgc aactagccac ttctttcatc
     9481 tctccttctc aagctctaat caatctacct caagaagatt ttgatagctg taaagcaagt
     9541 gcccaagcgg attgggaaaa tctcctccat cgttttgatg ttatagagac aggagaggct
     9601 gaccgaacct tctttgacca ctgcctctac agactcttcc tcttcccaca gactttttat
     9661 gaggttgatg agtcagggca agccatccac atggatctgg ctactggtac tgtcaagcct
     9721 ggtgtcctct ttagcaacaa tggtttctgg gataccttcc gcaccacctt ccccctcttt
     9781 gcccttatca taccggagca ctatcaacgc tttttagaag gtttcctcaa tagctaccgc
     9841 gatactggtt tccttccaaa atggctggct ccagatgaac ggggtatgat gccaggtaca
     9901 cttttagacg gtattatcgc agatagcgcc tgcaaggaca tggcccctga cctagaagga
     9961 gaactcttcc aagccatgct cgaaacagcc agcaaagccg accctctcgg catcaatggc
    10021 cgccacggac tagcccaata ccaagaacta ggttacctct ctaccgacca ccacgaaagt
    10081 gttagccata ctctagacta tgcctatagt gacttttgta tcgccagctg tgccaaaaaa
    10141 ctagagaaca tagaaatcgc tgaaacctac aaggctgctt cacaaaatta ccgccagcta
    10201 tttgacgctg agacaggtta catgcgagca cgagacaatc aaggaaactt tcaccctgac
    10261 ttctctcctt atagttgggg gcgagactac gctgaatgct ctgccattca agctacttta
    10321 ggcgttctcc acgacatccc tggcttaatc caactgatgg gtggaaaaga aacctttagc
    10381 aactatcttt tgaaagcctg tcaagatgct cccctctttg aaacaacagg ctatggttac
    10441 gaaattcacg aaatgagcga gatggctact gctccttttg gacaaatagc catttccaac
    10501 cagcctagtt tccacattcc ttatctcttc cgctacagcg attaccctga ctacactgcc
    10561 cttcttatca agacgctccg tcagaaagct tttcacccaa gttgggaagc ctatcctgga
    10621 gatgaagaca atggtagtct ctctgcttgg tacatctggt cagctctcgg attttatcca
    10681 acctgtccag gaaaaccaag ctatgatctc ggaattcctc tctttgacca tctacgagtc
    10741 tacctagcta aagaaaataa atggctagat attcatacta aacaaaacca caaccatttt
    10801 aactttgtca aagaatgccg actggacaaa acactcgtat caactattca acaccaagac
    10861 ctcctaaaag ctgaacaact aactttcacc ctcagctggc taccaaatca ctaataacca
    10921 aaagaacctt tgcatcgcgc aaaggttctt cttttatgaa acttggactt gaagttgatc
    10981 cacaagctga ccatccaccg ttaggttcag atagaaatcc aaggttttat ctcctgcgaa
    11041 atacaaggtt ggtagcgtca gccttttttc agtctcacca gcttgaaacg caaggacata
    11101 aatctcgtcc tgataggcga caccatggac acctgtcccc ggttgaatcg aaatcttagc
    11161 ctctaaagag gagctacttt cactacgttc aatcctaaaa tgaagagtct ctccctttgc
    11221 cacagctagg cttttttctg caaatgctag ttcctgaaca acttcttttt ttgataaccc
    11281 aggagtttta taaagggaaa tcttggtcaa tacagggata gattgtgcct cggtaatcat
    11341 cacgcgcacc ttttgtgcct ctactagcgg acctcgcaag aggcgcttat gaccaactgt
    11401 gaagcccctg ccaaactcct gccagacacc atccacctct acttgcacat gaaaagcagc
    11461 gattcgttgc cctagcttca aatcttctct taactcaatt acatcaaaag ttttaggaga
    11521 acctaaatcg agttctaact ggatgggcaa gtctgcatcg cttgcccaag agctggtctc
    11581 aaggccgtct gtcaaatggc gacaatcaaa gtctgcggaa agagctggac cagatacctc
    11641 agctcccaga gccaaatctt ctttatagag ctcattgcga taggtcgcaa attcataaag
    11701 tcgttcaata tcctttgcat caaagagccc agcttgattc ggcggaatat taagcaagag
    11761 tggagttcct cgccctactg agtgaaagta gatttcgacc aactcctcga gagacttagg
    11821 atcctgatcc tcatggtaaa accagcctgg acggatggaa acatctgcct ctccgattga
    11881 aaaaatcgtg cccgagggat ccccgtgctg aagatagttc agctctgctt ctgttcctag
    11941 tttatcaaga ttcacctttt gccacagtgg atcacctgca taccctcgtt cattgccaat
    12001 ccagcggata ctggtgcctt ctgttgaaaa aatcaagcaa tcgccctgca ggtcacgaat
    12061 ggtttcaaac catttttcaa attcataatt aaccttttgc gcgccctctc ctctggcacc
    12121 atccatccaa acctcagcga acttaccagc attcccatag ttaggatttg ataagatttc
    12181 cttcaactga gccagataat aggcattgta gtccgcttct cggtccacat gatagagggg
    12241 actatgggca tcccacggtg acaggtagac ccccatatcc atatcaaact ctgtggcagc
    12301 ttgggatact tcaaggagca agtcgccctt tcctctcctc caaggactga ccttaaccga
    12361 ataatctgtg tgagctgtcg gataaaggac aaagccatcg tggtgcttga ccaccaaaat
    12421 caactttttg aagcccgttt ccttgagcac acgaacccac tcacgcgcat ccaactgact
    12481 cgggttaaag cgctcaggat cctcctgtcc agtcccccat tcttggtcat aaaaggtatt
    12541 aggaccgaag tggataaaag ctgctagttc atctcccaga taagctagct gagtctgact
    12601 tggtaacggt ccatgcggtt tgattttctt catcgttata tatctccttc ttttgcaact
    12661 tctcttgttc acaagattat ccacaggttg tggataataa accatgatct aacagccagc
    12721 tctttccaac tccaactggt aggctcagga tttttctcct caatgttccc tcttcaaaca
    12781 gctctgtcat atggttctgc taacacctct cagaatgcta ctctccataa ttattctcag
    12841 gcttctttca ggtcattgtg tgaagaccat tcactatcca gacttttcca cagattgtga
    12901 ataagtgatt attctaaact atcatctcgt cgactataac aaaaaataaa aagagggtca
    12961 ataagataat tatctcttcc taaatactca gaaaagattc tataatttgt acaattgaac
    13021 aagaaatgct tctccaaaat ctatcttcaa aagaatgttg catcttttta tcctttcaca
    13081 caaacatttc tctttataca gatccaagtg ataaccagat gataattgga cactttttgg
    13141 tcatttgtat attttttgaa aaatttttta aaaaaatttt atatcctctt taatagaagt
    13201 ttatgcattt gtatatatgt attccatcct atgttatgta taataacata aaattgtgct
    13261 aaatatcata atttatatta ttttccgaga aattatagcc attgacatta cctaggaatt
    13321 gcgttataac atacatgtaa gcggtaccca aaatggtgcc aagtcaaaat ttttaaggag
    13381 gaaaatacat gtcttcacat ccaattcagg tcttctcaga aattgggaaa ctgaaaaaag
    13441 ttatgttgca ccgtccaggc aaggagttag aaaacttgtt gccggactat cttgaaaggc
    13501 ttctttttga tgatattcct ttcttggaag atgctcaaaa agaacatgat gcatttgccc
    13561 aagctcttcg cgatgaagga attgaggttc tctatctaga acaactcgct gctgaatcat
    13621 tgacctctcc agaaatccgc gatcaattta tcgaggaata cttagacgaa gccaacatcc
    13681 gtgatcgtca aaccaaggtt gctattcgtg aattgcttca cggcatcaag gacaaccaag
    13741 aattggttga aaaaacaatg gctgggattc aaaaagttga attgccagaa attcctgacg
    13801 aagctaaaga tctaactgac ttagttgaat cagagtatcc atttgcaatt gacccgatgc
    13861 caaacctcta tttcactcgc gacccatttg caacaattgg aaacgccgta tcgcttaacc
    13921 acatgtttgc agacactcgt aaccgtgaaa cactctacgg taagtatatc ttcaaatacc
    13981 acccaatcta tggcggaaaa gtggatttgg tctacaaccg tgaagaagat acgcgtatcg
    14041 aaggtggaga cgagctagtt atttctaaag acgtccttgc agtaggtatc tctcaacgta
    14101 cagacgcagc ttctatcgaa aaacttttgg tcaacatctt caagaaaaat gttggcttca
    14161 agaaagtttt ggcctttgaa tttgctaaca accgtaaatt catgcacttg gatactgtct
    14221 tcactatggt agactatgac aagttcacta ttcacccaga aatcgaaggc gaccttcacg
    14281 tttactcagt tacttacgaa aacgaaaaac ttaaaatcgt tgaagagaaa ggtgacttag
    14341 ctgaacttct tgctcaaaac cttggtgtag aaaaagttca tttgattcgt tgcggtggtg
    14401 gcaatatcgt agcagctgcg cgtgaacaat ggaacgacgg ttctaacact ttgaccatcg
    14461 cacctggtgt ggtagttgtt tatgaccgca ataccgtgac caataagatt ttggaagaat
    14521 acgggcttcg cttgattaag attcgcggaa gtgaattggt tcggggccgt ggtggacctc
    14581 gttgtatgtc tatgccattt gaacgtgaag aagtgtaatc gctgttcggt attcgtcaat
    14641 agaaaatgta aaaaatagaa agaggaaata ataaaatgac aaattcagta ttccaaggac
    14701 gtagcttctt agcagaaaaa gactttaccc gtgcagagtt agaatacctt attggtcttt
    14761 cagctcactt gaaagatttg aaaaaacgca atattcaaca ccactacctt gctggcaaga
    14821 atatcgctct cctatttgaa aaaacatcta ctcgtactcg tgcagccttt acaactgcgg
    14881 ctatcgacct tggtgctcac ccagaatacc tcggagcaaa tgatattcag ttgggcaaaa
    14941 aagaatctac tgaagatact gctaaagtat tgggacgtat gtttgacggg attgaattcc
    15001 gcggattcag ccaacgtatg gttgaagaat tggcagaatt ctcaggcgtt ccagtatgga
    15061 acggtctaac tgacgaatgg cacccaactc aaatgctcgc tgactacttg actgttcaag
    15121 aaaacttcgg tcgcttggaa ggcttgacat tggtatactg tggtgatgga cgtaacaacg
    15181 ttgccaacag cttgctcgta acaggtgcta tccttggtgt caatgttcac atcttctcac
    15241 caaaagaact cttcccagaa aaagaaatcg ttgaattggc agaaggattt gctaaagaaa
    15301 gtggcgcaca tgttctcatc actgaagatg ctgatgaagc agttaaagat gcagacgttc
    15361 tttacacaga cgtttgggta tcaatgggtg aagaagacaa attcgcagaa cgtgtagctc
    15421 ttcttaaacc ttaccaagtc aatatggact tagttaaaaa agcaggcaat gaaaacttga
    15481 tcttcctaca ctgcttgcca gcattccacg atactcacac tgtttatggt aaagacgttg
    15541 ctgaaaaatt tggtgtagaa gaaatggaag taacagacga agtcttccgc agcaagtacg
    15601 ctcgccactt cgatcaagca gaaaaccgta tgcacactat caaagctgtt atggctgcta
    15661 cacttggtaa cctttatatt cctaaagtat aattttagat aataaaccgt ctaccaacag
    15721 ctatgagggc tgcgactaat agctttagtc cggtcctctt ttatataatg gtaatctatt
    15781 atttcttata aaatatgtga aaaatcatta aattgaaatc taaacgcatt ctattgagtg
    15841 tgataaagga gaatttatgg caaatcgtaa aattgtagta gctttgggag gaaatgcgat
    15901 tctttcttct gacccatcag caaaggctca acaagaagct ttagttgaaa cagctaagca
    15961 tcttgtaaaa ttgattaaaa atggagatga tttgattatc actcacggta atggacctca
    16021 agttgggaat ctcttgctcc aacatttggc atcagactct gaaaagaacc ctgccttccc
    16081 actcgactca cttgtcgcta tgacagaagg tagcatcggt ttctggttga aaaatgcttt
    16141 gcaaaatgct ctcttggatg aaggcatcga aaaaaatgtt gcctctgttg taacgcaagt
    16201 tgtcgtagat aaaaatgatc cagcttttgt taacttgagt aaaccaatcg gtcctttcta
    16261 ttcagaagaa gaagcaaaag cagaagccga aaagagcgga gcgactttca aggaagatgc
    16321 tggccgtggc tggcgtaagg tcgttgcctc accaaaacct gttgacatca aagaaattga
    16381 aaccatccgt actcttttaa ataatggtca agtcgtcgta gctgcaggtg gtggcggtat
    16441 tcccgtcgtc aaagaaaaca atggacattt gactggtgtc gaagcggtta ttgataaaga
    16501 cttcgcttcc caacgtttgg cagaattggt tgatgcagac ctcttcatcg ttttgacagg
    16561 tgtagattat gtatttgtta actacaacaa gccaaaccag gaaaaattgg aacatgtgaa
    16621 tgttgcccag ctggaagaat atatcaaaca agatcagttt gcaccaggta gcatgcttcc
    16681 aaaagtagaa gcagctatcg cttttgtcaa tggtcgtcca gaaggaaaag cagttattac
    16741 ttcccttgaa aatctaggcg ccttgattga atctgaaagc ggaacaatta ttgaaaaagg
    16801 ataagttgtt ttactaataa gatgtattct atttctagta tctttatatc aaattagaaa
    16861 ttattcttga aaacatgtac aatatttcaa aagatactag ttttagactt taatatggta
    16921 aaacaaatat aaatagaaag cgttttcttg aatgtttatt taagaaagta gttggttttt
    16981 tacactttgt tagacatcag gaggaaaaac aaatgagtga aaaagctaaa aaagggttta
    17041 agatgccttc atcttacacc gtattattga taatcattgc tattatggca gtgctaactt
    17101 ggtttatccc tgcgggggcc tttatagaag gtatttacga ggctcagcct caaaatccac
    17161 aagggatttg ggatgtcctc atggcaccga ttcgggctat gctaggtact catccagagg
    17221 aaggttcgct cattaaagaa acgagcgcag cgattgatgt agccttcttc atccttatgg
    17281 ttggtggttt ccttggcatt gtcaacaaaa ctggtgctct tgacgtaggg attgcctcta
    17341 tcgtgaagaa gtataagggc cgcgaaaaaa tgttaatttt ggtactgatg cctttgtttg
    17401 ccctcggtgg tacaacttat ggtatgggtg aagaaacaat ggccttctat ccactccttg
    17461 tgccagttat gatggccgtt ggttttgata gcctgactgg tgttgcaatt attttgctcg
    17521 gttctcaaat cggctgtttg gcatctactc tgaatccatt tgcgacaggt attgcttcag
    17581 cgactgcggg agttggtaca ggggacggta tcgtacttcg tctgatcttc tgggttacct
    17641 tgactgctct tagtacttgg tttgtttacc gttatgcgga taagattcaa aaagatccga
    17701 ctaagtcact ggtttatagt actcgcaaag aagatttgaa acactttaac gtagaagaat
    17761 cttcatctgt agaatctaca cttagcagca aacaaaaatc agttctcttc ttatttgtgt
    17821 tgacattcat cttgatggta ttgagcttca ttccatggac agaccttggc gttaccattt
    17881 ttgatgactt taatgcttgg ttgactggtc ttccagttat tggtaatatt gtcggttcat
    17941 ctacttctgc actaggtact tggtacttcc cagaaggcgc catgctcttt gcctttatgg
    18001 gtatcctgat tggtgttatt tatggtctta aagaagataa gattatctct tccttcatga
    18061 atggtgctgc tgacttgctc agtgttgcct tgatcgtagc gattgctcgt ggtattcaag
    18121 ttatcatgaa cgacggtatg attaccgata caatcctcaa ctggggtaaa gaaggcttga
    18181 gcggtctatc ttcacaagtc tttatcgttg taacttatat cttctatcta cctatgtcat
    18241 tcttgatccc atcttcatct ggtcttgcca gcgcaactat gggtatcatg gctccacttg
    18301 gagaatttgt aaatgtccgt cctagcttga ttatcactgc ttaccaatct gcttcaggtg
    18361 tcttgaactt gattgcacca acatctggta ttgtgatggg agctcttgca cttggacgta
    18421 tcaacattgg tacttggtgg aaattcatgg gcaaactcgt agtcgctatt attgtagtga
    18481 ccatcgccct tcttctcctt ggaaccttcc ttccattcct ataaaatagt gagtgaggtg
    18541 attccatgaa aatagatata acaaatcaag ttaaagatga atttcttata tcattaaaaa
    18601 ccttgatttc ctatccttca gtactcaatg aaggagaaaa tggaacacct tttggacaag
    18661 caatccaaga tgtcctagaa aaaactttag agatttgtcg agacataggt ttcactacct
    18721 atcttgaccc taaaggttat tacggatatg cagaaatcgg tcagggagca gagcttctgg
    18781 ccattctctg tcatttggat gttgttccat caggtgatga agcagattgg cagacaccgc
    18841 catttgaagc aactatcaaa gacggctggg tattcggacg tggtgtccaa gatgataaag
    18901 gcccttcgct cgcagctctc tatgcagtaa aaagcttgct ggaccaaggt attcagttca
    18961 aaaagcgcgt acgctttatc tttggtaccg atgaggaaac cctctggcgc tgcatggcac
    19021 gctacaatac catcgaagaa caggccagta tgggctttgc acctgactca tcttttcctc
    19081 tgacctatgc tgaaaaaggg cttctacagg tcaaacttca tggccctgga tcggatcaac
    19141 tagagcttga agtaggaggc gcctttaacg ttgtaccaga caaggccaac taccaaggtc
    19201 tcctctatga acaggtttgt aacggtatca aagaagctgg ttatgattac caaaccactg
    19261 aacaaaccgt aacggttctc ggagtgccaa agcatgctaa ggatgctagt caaggtatca
    19321 atgctgtcat ccgactagct accattcttg ctcctctcca agaacaccct gctctcagtt
    19381 ttcttgcaac acaagcaggt caagacggca caggaagaca aatctttggt gatatagcag
    19441 atgaaccttc tggtcaccta tcctttaatg tcgcaggtct catgatcaat catgaacgtt
    19501 ctgaaatccg tattgacatt cggactcctg tcttagctga caaggaagaa ctagtaaagt
    19561 tgcttacaag atgtgcacaa aactaccaac tccgctacga agagtttgac tatctagcgc
    19621 ctctatacgt cgcaaaagac agtaaactcg ttagcacact gatgcaaatc taccaagaaa
    19681 agactggcga taacagtcct gctatttcat ccggtggtgc cacttttgct cgcaccatgc
    19741 caaattgtgt agccttcggc gccttattcc cagaagcgaa gcagacagaa catcaggcaa
    19801 atgaatgtgc cgttctagaa gatttgtacc gtgctatgga tatttatgcc gaagccgtct
    19861 atcgacttgc aacttaatca ggcaactgtt tctaccaaaa aaatcgaccg attaatgaac
    19921 tgcaccccaa aagttagaca gaataaatct aacttttggg gtgttttatt atgaaattga
    19981 gttatgaaga taaagttcag atctatgaac taagaaagca aggacaaagc ttcaaacagc
    20041 tttcaaaaag atttatgtgg atgtttctgg tctaaagtca tctgaatctt tgagatgagc
    20101 tttataaatc gcttttttca gtttttgcac tggtgtttcg ataaactcaa actttttagc
    20161 cgtggtattg cctgatttta tagtatattg aaactagaat agtacacctc tccttctaaa
    20221 acatttttag aaatcgattt gactgtcctg atcgatttgt cctgttctta tttcatttta
    20281 ctatatttga gccacttcgt ctttaacggc tttattcata agcttttgta atttttcttt
    20341 actatcaatt acttctgatt ttccgttgta atttattgta ataggtttta acttacctaa
    20401 tttctcgaca cgctcattaa tttgatcttt tttgaaggct gcatatgttt ttcctaagat
    20461 tttttcgaaa atatatttat cagatagcgg tttgccttct tcttcagttt ggtttttgta
    20521 ttgatttgaa acataaggaa caaatccttc atagtaacct aatgctccca taagttcaaa
    20581 agcttgtttt ctaattcaaa ccattgcaac tcagatttca gcttttcaga taaatcctgc
    20641 tcatccaaat aatgacttga aattagtgct gaactcgttt ctgtagcctg tacaggctga
    20701 gcacccatac cagcaaaaaa taaactcgtt cctagcaaga ccgaacaagc tcctattgca
    20761 tatttcctca aagaaaaacg ctgctttctc tcaaattgaa attctttcat cccatctccc
    20821 atcattcatt attactgtat attttgtata tcagaaatag tttgtattca caaatctttc
    20881 tagttattcc cttatcattc ctaattaagg gagatgacat acaataattt ttagttaaat
    20941 gtatatcgat gttttttgtt tttcttaata aacgcaatac aaaaagagcc tgttgccaag
    21001 ctctttgcac tcaatgaaaa tcaaagagca aactaggaaa ctagccacag gttgctcaaa
    21061 acaccgtttt gaggtagtag ataagactga cgaagtcagc tcaaaacact gttttgaggt
    21121 tgcagataga actgacgaag tcagtaacat ctatacggca aggcgacgct gacgtggttt
    21181 gaagagattt tcgaagagta ttagtctatt atttcttctc agcgcgaagg gctgacaaga
    21241 tttgtgttcg gatatcatcc acaccatttg gagtatttgg taaaaagatg gtttgatttc
    21301 ctttagaggc aaaggtattc aaggtatcca aatactggtt ggtcaagagg atagacatga
    21361 tttgttcttc tgtcatgcca acattggctt ccttgagttc ggtgatagac tctgccaatc
    21421 catccacaat cgccttacgt tgttgggcaa tccccacacc atgaaggcgg tctttttctg
    21481 cttctgcttc agctgcagtg acaattttaa tcttgtcagc ttccgccaat tcttgtgctg
    21541 cgacccgctt acgttgcgcc gcattgattt cattcatgga ttgcttaact tctgcatctg
    21601 gttcgacctt ggtaatcaag gttttcacga taatgtagcc gtaagtggtc atttcttctg
    21661 ctacttggtg ttgaacctca agggcaatct catctttttt ctcaaacaat tcatccaagg
    21721 ttaattttgg aacagaagag cgaagagcat cttcgatata agatttaatc tgagattctg
    21781 gacgtatgag tttatagtaa gcatctgtca cgctctgctc gttgacacgg tactgagtcg
    21841 ctacattcat cataacgaac acattgtcct tggtcttagt ctcaaccaca atatcacttt
    21901 gcaacaagcg caactgaatc cgtgctgcaa tcgagtcaat cccaaaaggc aagcgaatat
    21961 gaataccgct attagcaacc ttttggtatt tcccaaagcg ttcaataatc gccaccgact
    22021 gctgacgaac cacataaact gtactcagtg tgactatcac caataggagc acacaaacaa
    22081 tcagaaaaat catgaaaaat attgccataa tggaacctcc acaagtattt ttctagtatt
    22141 atagcacatt taaagaaggc tgtgccgttt ttactgcgat ttttcctgaa atgtcaataa
    22201 ttagaggtga attgtcctat tgtcgtccaa tctcttgcta aaataactct ttataaaagg
    22261 caatcgtttc ttctaaggtt ggcataaatg gatttcctgg tgcgcaggca tcaatcaagg
    22321 cattcttaga aaggtattca aagtcgaaat ctttttcttc aataccaagt tcagtcagtt
    22381 tcttaggaat acctactgtc tcagaaagct tctcaatctc agcaatcgca taatcggcac
    22441 attcttgatc tgatttacct tctacatgaa gtcccaaggc tttggcaaca ttgcggaaag
    22501 cttctggtac acgtttagca ttttcacgtt ctataactgg tagcaacatg gcacagcaca
    22561 cgccatgtgg caaattatat actgcaccga gttggtgagc cattgagtga acataaccaa
    22621 gaccagcatt attaaagctc atatcaccaa ggaagatggc attgaccata ccttcacgcg
    22681 cttcaatatc atgtccattt tctacagcac gaggaagata ttccttgata agctcaatag
    22741 caccaataga cagtttcttg gttacatcat aagcaccagg tgttaccaaa gcttcaacag
    22801 cgtgagtcag agcatccata ccagtagcag cagtcaggcc tttaggtttg gaaagcatga
    22861 gttccgggtc attaacagag ataagagcaa ggctattctt gtcaaccatt accatcttaa
    22921 ccttgcgttc ttcatcagta atcacatagt taatagtgat ttctgcagaa gttcctgccg
    22981 ttgtattgat agccacaact ggcaagcctt ttttagcaga cttatgaagc ccttcataat
    23041 cctgtggttt tccaccattt gtagccatga tagaaataca actagctgca tcctgtggag
    23101 aacccccgcc aagactgatg ataaagtcac agccatgttc ttgcaaagca gctaccccat
    23161 ctgtgacatt tttacaagta gggtttggct ctacatcgct aaagatgaca tattcgattc
    23221 cttctgtatc cagtggtttt aaagtcttag gtaaaatatc actgccttcg atgtacttat
    23281 ctgtaaccaa aagtgccttt ttatagccaa gttccttgat ataaggaccc acttcattta
    23341 caacaccttt tccaataagg ttgactgccg gaacataaaa tgtagccata taattttcct
    23401 cctgcgcctc cgcgctatct atttaacatc attatattac tttttgtatt cgtttttaat
    23461 tatttgtgaa ttgtttaaca aatcttttta aaaggatagg agggacgtaa gttcacctcc
    23521 tatataaaca agttttattt atgtagtggt cccaacaact gacatgcaca atagtctgct
    23581 gattctagat cttctgttcc aaataaggac caatttttag gtctaaagat atcttcctca
    23641 ggtacattat gcatattgac aggaattctc aacatagaag ccaaggtaat caagtctgct
    23701 ccaatgtgtc catatgttat ggctccgtga ttagctcccc aattattcat gacgtcatag
    23761 acagacttga aagcaccttt tcctgtcaaa cgtggagcaa accaagtagt tggccatcct
    23821 ggatctgtac gattatctaa agtatggtga acatcttcag gaagttcaag tgtataacct
    23881 tctgcaattt gtagcactgg accaacccct tttagaagat tgagacgtac cattgttact
    23941 ggcatatccc ccttcgtcaa gaaacgagtt gagaatcctc ctccacggaa gtattcgcgg
    24001 tttgctggtg ggaagtctgt attttcaagc atagcctgca cttcactttc ttccaactcc
    24061 cagaatggtt tcataacagg tttgccatct cgagtagctt gacctgtacc atccaatgta
    24121 caagaaccag agttgattag atgtaagaag ccagctgcag cacgaccctc taaagtatgt
    24181 cccgttacac gtttaacagc ctctgggctc caataagtac gcacatcagc aaagatttgt
    24241 ggagtatttg ttaatagata attaaagagc atagacacac catttagtga atcattctct
    24301 gtcgcaaata caaatggttt tcgaatacca ttccagtcaa actgagtatt gaggaaagtt
    24361 tccataaaat ctccatttgg aaaatggtct gtccactgac gttgaccttg gaaaccagct
    24421 actaaagcat ggtgaccaac tgcttcttcc tcaaaaccaa gttcagcaag tcttgggtta
    24481 ccaaccatta agtcacgtcc aatcatgaac atcttaataa caaattccca ttgtctatct
    24541 ttttcttcac ggcttaaaac aaggtcttca cggttatggt cgaatccttc ttttacgttt
    24601 tctttcaccc atttgagcgc acgttcgaac tcttcagggt cgtaaatact acgatccata
    24661 cggcgcgtga actccgtcat atctaccgat tcatttcgca ttcctaggta ttcttggaag
    24721 aaatccggat ttacaataga accaccaatc cccatcgaaa cactacccat tgataggtaa
    24781 gcagtgtctc tcatcaagcc agttgcaaga gctgcacgcg catagcgtaa aagtttttct
    24841 ttgacatctt ctggaatatc tgtgtcacta acttcctgaa catctcttcc ataaatccca
    24901 aaggctggaa tccctttttg agcatgtgaa gctagtacag ctgcaagata gacagctcct
    24961 gggcgttctg tcccattaaa tccccaaata gcatgaggaa tatctggaga catatccata
    25021 gtttcactac cataacacca gcatggtgta actgtaattg ttgcgcaaac atttgatttt
    25081 ttaaacaact catgggaagc tgcagcctct ggtacacggc caatagtaga tggagaaatc
    25141 acgcattcca caggttcccc atctggatat ttcaatgtgc ttgaaatcaa atctgccaca
    25201 cttttagcca tgttcattgt ttgcacttca agtgattcgc gtacaccttg acgacgacca
    25261 tcaatagtcg gacgaatccc aatacgtgga tgttgaatca tactattttc ctcttatttt
    25321 tctgtttctt tcatactacc atctccatta tatttacggt agcctgggtg ataattttta
    25381 aaattactta taagtataaa cctctacctc tgctaaactt aaaggaattc ctgcgcttct
    25441 caaagaaatt ctaacctttt ttcccttcag tccttttaag tctatggata gattattgct
    25501 ttctaaactg tcaatatgtt gtgtaaaaac ttcataatca tttgaatcat atataataat
    25561 atcaaaattt gataatctct gaatttcagc atctgttcta ttataaataa ttaacttttc
    25621 taattcttcg gtttgtgcta aatctatctc ccaccatgaa ggagaatctt ctttggtatg
    25681 agtcactgaa tgatgaccat aatctccgtt tttatttcca tcaactgcta gacgagaaaa
    25741 accattgtaa tccgttgaac tctgtcgagt ttcttttgtt aatgcaatat tagaaagttt
    25801 cttcggagtt ctctttgaac catagacttc aacttccgct aaacttaacg gaatagctgc
    25861 tgattttaat tctattttga ctagcttagc ccctacagaa ggtactttta aagataacaa
    25921 accatcaact actttatcaa aatgtcttct aaaaacttct tctcctgatg aagatagaaa
    25981 aataacatca aaattagata aacgctgagg ttcggcatct gttctattat aaatatcaac
    26041 tttagaaacc gtaaactctt ctcccagatc gacctgccac caagcgttag aatctgcctt
    26101 agtatgagtc accgaatgat gtccgtaatc tccatcttta ttaccatcaa ctgctaaagc
    26161 agctacaccc tcataattgg ttgaactttg agtagctacc ttatttgtag agactacttt
    26221 atcttctgtt attttatcta tatcctcttc agattgctta ccatctgatt ctctaaaaac
    26281 ctctacttct gctaaactca aaggcactcc actcgttagt aatttaactt taatatacct
    26341 tgctcctttt tctttgaaat ctagactaac agattcaccc gacaaattat taacatgttt
    26401 cttagcaact tcgtttctat tattgtcata tagaatcaca tcaaaattag atagacgttg
    26461 agtctcagca tctgtgcgat tataaatttt aacaagccca actttatcca tttttttcaa
    26521 atcgacttcc caccaagagg gattatctgc cctagtgtgt gttaccgaac cagagttaaa
    26581 attaccattt ctgttaccat ccacagctct attagggtct ccattgtaat ctacagaact
    26641 ttgtgttgtt ggttttgcat atgctatatt caaattacca tcattaaatt tatctggtgt
    26701 tgggaaagag actggacctg tcccctcagt atttatagac atctctacca ttccattatg
    26761 attaaccgta atggtataaa catgggtatt ctcatcccaa ttttctgtat aagtataatg
    26821 atttttatcg ccacttatat ttatctgagg tttatgacca gtatgccctt ttaatgtaag
    26881 tgttgttgtc ctaaagtcat tatctacagg attgatggaa taatttttgc ttatccagtt
    26941 cgctaactct aattcttctt tcttccaact ttttgatgca tcaaaatttc ctgataatgc
    27001 ccacatagct gtcttatctg tcctgtaatt attcaataaa atatgtaaat tatttggatt
    27061 ttctttaaca acagcgtaag tatgtggcgt caaatctaac gataacgact ttgtattatt
    27121 agtatacata ggcaacatta cttgctgatt tttattgata ttagcattac tattataaat
    27181 ataccaggaa ttacctacac gctgagcata cccatctcct tcataaagtt ttggataaag
    27241 cgagtttaaa tagttgactt tactagacaa ttcctcacta tttttagtca aaatttttgc
    27301 attagggaat atagatgaaa tcttttcctt atcaattttc tcatgtatta caggaagaat
    27361 atgatatctc ccattattat ataaaggcat tgtttcatca ttcgaataaa gtccttgata
    27421 aaatccgttt aatgaactaa tcctaccttc tccattccaa aatacagctt ttgttctatt
    27481 tacaacttct tccttacttg gagctggatt ttgtatagca tgtctaaaga aaggaataat
    27541 acctttagta aatgctggag ttggtacatc atttgtcata aatgtatacg cggcacattc
    27601 gaaattataa actgtgcctc ccccagtata tacattcatc atttccatag caatcattga
    27661 ttctggttcc gatgcatagg atctcatatc cctagctctt ccagtttcaa atgtgtttgt
    27721 ataatgtttt tcccaccatt tccatgtatc tgttgatgag ccccagttat cacataagcc
    27781 actcaaccaa aatccactaa cgatagaatc tgtacccgca tcatctctta ttggcgtatt
    27841 tttagttgcc aacaccaaat ttttatgata tttttgactc gcttcaaaga atgtcggatc
    27901 attcataata gtttcccaga accatttttc atgatcatgc cagataaaat gcgctccata
    27961 tttggcacaa acttccaaat atttagcact atgcggagct aactggttat tgtaaatcca
    28021 ataattctca atatttaaaa caccttttaa cacactatac ttttggaatt gttcatctaa
    28081 ccactctgga ggaactgtat tacgctctcc agccgacata ataaccaaga aaactggaat
    28141 gtttagactt tgagcttcct ccaacatctt gacataccat tctctcaatt ctttcgtatc
    28201 tcgtggaata caacttgttg gtttacagac ttttgcagga tgaagttcaa tcgctgcata
    28261 tggctttaca tcttcaggaa tagcttccca tgctcccttc aatgtgttcc cccaccataa
    28321 tccgttacca ttatcattgc catacaacgg agaaagcaac aatggcgatt cattattaat
    28381 cgtcgttctc atttgaacac gattatcagc cttaactcct gctaacaatc caaaacataa
    28441 catcccaata catacaaaca atattgtgat taattttctt tttatttttt ctttattcat
    28501 gacttatacc taaaaattta actcaataat tttttctgaa ttaaatacca attcaatatt
    28561 ttgtacatca gtatttttgc catatattct tactcttact ttttgatctt tgtttcctcc
    28621 ttccaatttt aggaatgtta tatccccatt tttccaagca aacgatacct tatatcctcc
    28681 tcttactctg aaacctttca cttctccttc tgaccaagca gaaggtaaag ctggaattag
    28741 tgataaccaa ttatgatggc tctgtactaa taattcacaa attccactca ccaaacctaa
    28801 attaccatca atttgaaatg gtggatggtc aagaaataaa ttgccaagag tcgcattatt
    28861 taacaaacca ttaatctggt tataagcagg ttcaccttga tatagtctcg caaaaaaatg
    28921 aatcagccat gcagcactcc aacctgtttg tgtactagca tgcaaaccac ttactaacca
    28981 attattaatc gcttgctccc tctcctgtga agataaaaaa ttagcgtttg ataatctcct
    29041 attgatagtg attttagctg cttctgctaa ttccggagtt ttatgaatat caatctcatt
    29101 ataaggataa agcccaaata gaggtgaaat gtgtctatgc ccaggctcta cttcttcata
    29161 atcttctaac cattcttgga tttgcccatt actacctatt tttgttttag gtagtttctt
    29221 ttttaactcc ttcacacgac taataaaatc cgaattgtct cctaattgtt ttgcaatgcc
    29281 aatgcatgaa tcacaaaaat atcttagaat ttgattatca attgtagatg atagacaagc
    29341 atttccttca ataccatttt ttaagcgata tttattttcc ggtgagacac ttggacctgt
    29401 catcaagtag ccatccacct caaataaata atcttcaaag aaaagaaatg cttcttttat
    29461 catttcaaaa tgttccgtaa gaatacgctc atcttggaaa tataaatagt gttcccaaat
    29521 atgagtacat aaccatggaa tagttaatac ccaaattgca gcccccatgg catgagattg
    29581 gggagccgta tcgccaaaac catccgtatt atgatgtgct gtaaaacctc tagctccata
    29641 cattttctta gcggttagtc ttcccggttc tctcattctt tcgagcatat caaataatgg
    29701 atattctact tctggtaaat cacatggacc taccatccaa taattcattt gagtattaat
    29761 attaatcgta tatttagaac cccaaattgg atttaattca tcacaccata ttccttgaag
    29821 attggcaggt aaaccattcg gttgactaga cgatattaac agatatcttc cataatgaaa
    29881 taacaagtta gtcaagtagt tactatactt tttagtgttt tcaagaagta gattcgttgg
    29941 aatgctaaga caacctttac tatagtctag tttaaaatca actctattaa attgctcctg
    30001 atattttttt acatgttcat ctttttctgt aaagtaatca atactactaa attctccctg
    30061 aagagaagaa atatctatat ttccccaata atccgtcatt gatttgagat aaagaaatac
    30121 ctctgtagca ttccgaataa ctattgtctc tcccaataca cttacttcac catccgtaac
    30181 cttagaatga catactactt taaactgaac accttttcta cctccagcag aggccgacat
    30241 taaaattgta cttgaatcca gtttagatac ttcgtcatta aaccgtttat ttctacccaa
    30301 attaatgttt aaatttaatg tgttttgaac tgatgacact atacggcaac ataaaatatt
    30361 cttattaaaa ctcgtaaaat attctctttt tatttgtaaa ttacaactat taggctcaaa
    30421 cacaacatta gaaatagctg tatctaaatc tagctctctt tcatacaatg aaagagcaca
    30481 agactgaata tctatatgct caatgtaaag ttccccaagt aattcatagt ggctttgatc
    30541 tcttggggta gcaaacactg ttaactttat caattcttcg gctttctgaa tttctccatc
    30601 taaaagatat tcccgaattt ttttaagatg caatagtgag tctggattat ttctatctga
    30661 ctttcctcta taccaaatag tctcatcgtt tagttgaata cattcttttg tagctgaacc
    30721 ataaatcata ccacctaaat gaccgttccc aatcggcaag gcttcattcc aatttgcggc
    30781 agctttctta taccataatt tcactcttta cctctcttcc aaaatggagg tgatatccca
    30841 aataccacct ccacactttc tactacttca aaattcctaa aacaactaga ataactccaa
    30901 tagccataac actaaagatt agtacaaccg gattctttcc ttttctaatt aatttacaca
    30961 ttaataaagt caaagccata gggataaatc ctggaattaa ttttgtaatc atttcttgaa
    31021 cagaaataac aagttccccc tgcttaaatt ctaatccaaa attaatacca accattgatg
    31081 gaatcaaacc acccactagt actagcccta atgctgtcgc catactcgta acgcgattca
    31141 atgttccttt tgtattattt tcttggataa gactagaacc cttagtatac ccatatttca
    31201 aaccgaaata tttaacagga atattaataa tattaaacaa tattaaggcg ataaagatac
    31261 ctaaagcacc gccgtcttta gaataagacg caccaatact aaaacagata ggaactagtg
    31321 tcagccagaa caaactatct cctagaccag ccagtggccc catcaagcca actttaatac
    31381 cagtaattga agctgcacct tcatttcctt cttgttcttc catagcggaa gtaactccaa
    31441 gaataaatgg tgccgctgtt tgatgagtat tgaaaaactc aaggtgacgt ttcattgcag
    31501 gagaagctga atctttgtct gggtatagtt ttttcaatac tggaagaatt gtatatagaa
    31561 aacctaggtt ctgcattctc tcatagttcc atgaagaacc atacataaaa cttcgtttga
    31621 aaactttttt aagttcaact ttagttactt tattattcat catagatcaa gatcctcctc
    31681 aacatcacta cttgaagttg tttcttgtgg tttgcttcca atcatatcgt agaagtatgc
    31741 taccgcaata ccaactagtg agatcccaat ggttgacatt cctccataca cagaacaaat
    31801 gaatccaatc aacaagaata cccacatatt tttcttgagc atcatattta aaagcatacc
    31861 aaaaccaatt gaaggtagta aagcacctgc agagttaagt ccctgcataa cgattggtgg
    31921 aaccttagca aacaagtctg ccacatattc ccctccaagc ataatagcta ggaaaattgg
    31981 tacaatttta aataacgtga ttaaaaccaa acttgaataa tgataaaaac cgatctttga
    32041 ataatctccg tttttagcat cattatcagc ttttttcaca aaataaacat ctaaagtttt
    32101 tgcaagaaca tccaactgtt gggtagcaac tgcaattgga actgctatag cgataccagc
    32161 agtttctcct tgaccagata aaataccaaa tgcagtacca ataatcgcac ctgaaatagt
    32221 atctggtgga gtataacctc caatacctgt tacaccgagc caagttaatt caagagctga
    32281 accaataaga acaccttggg tgaaatctcc taatactaag ccaaccattg cacttgtaac
    32341 taacggacgg tgaatcataa ataacggtcc attatagtca atagtggcaa taatcccaac
    32401 taatgtcacc agtaatgctt gtgtgaatag catatactgt acctccttaa attattttta
    32461 taaaaatttt tccaacattg taactttatc attaggaacc atttgaattt caactctcgt
    32521 tcccttatca actattttct taaaataata tttgtcttct tcacctagag ctacacctcc
    32581 cgttaccttt tcattttcct ccgttttact catctgtcct acattgaggt actccaattt
    32641 taaatttcct tcaatagaat catacacatc ttttggattt gtatatatca gcattgttct
    32701 cttttttatt ggcacagagt ttaaaacttc cacaaaccgt tttacactaa agaaaacaat
    32761 ttttaaacct accggtgcag aaatctttaa aatagattgt cgtgttttat cttctgagat
    32821 gcgatcatta acaatgataa cttgctcaat atcatacttt tttagccacg tagtgacaac
    32881 ttgaccatgt accagacggt catcaatacg aacaaactct atagacataa atcttcttcc
    32941 tcctctacgt taagttgttg tttaacatta aacaaactat tttgagcatt gtgaacaatt
    33001 tcttctaaat tgatttttga atcataactt gatattaatt ccactaggag agggatatta
    33061 aaccctgtta caatatcaac tgaatccaaa tttaaaaacc gtgacaaagc cacattatta
    33121 ggacttcctc caatcaagtc agtcaaaacg ataacctctt gatttgagtc taacagttca
    33181 tccacttgat ttttaaaata atgctcaaac tctacaatat tctctccagg catcaaacct
    33241 aatgtcctaa ctctatcatt tgttgtctca ccaacaaaca tttctgcggt catgagagct
    33301 ccgctagcaa aattaccatg actggcaact aaaattcctc tattgaacat tttctctttc
    33361 aacaactact cctttcttaa ggataatatt agcataaagt gaagtttctc ctgtagcaac
    33421 aatagcataa gctttcttac tacgttcata aaagtcttct cttctaagat aagtaatcgt
    33481 tttaagatct gtaccatgac cttcaatcat ctgtctatag gtaccccata tcttaggaat
    33541 atcatcaccc gaaacaacgt tcataaactg aattgaacta tcgacgtaac tatctaatgg
    33601 cattaaatac agaatggaat ctaataattc tggaatattt acaccatcac aacgaattag
    33661 cttatttgca catgaggcag aaggataatt cgcgtcagct aatactattt catctccatg
    33721 tcccatttcc attaaagtct tcaataaatc tggagaaata tttttcggta tatgttttaa
    33781 cattaatatc ctccctattt tctctgaccg tatgttttaa atttttctgc catcaattcc
    33841 atctcctcat caggaagaac tactggctct ccaaaattct tagctaaaca ataaattttt
    33901 gcacaatatt caacttcttc aacaatatta aatgcattca ataaattttg tgcacctgct
    33961 aaaattccat gattcgctag taaaactgct ctacgacctt ccattgcttt agctgcattc
    34021 acagccaatt ctttcgtgcc atatgttgca tactcagcta cccgaacatc tttccctgcc
    34081 actgcaatca tataatgact cgctggaagt ggttctctga gacaagctaa tactgttgca
    34141 taagttgtat gagcatggat aattgcatcg atatcatcac gagtttgata ttgaatcaaa
    34201 tgcatatacc attcgctaga tggcaagcgt tctccctcta caacatttcc attaatatcc
    34261 atcactacaa tatcggattc tttgatttca aagaaatcaa tacccgacgg ggtaattgcc
    34321 atcaattgtt tttcacgatc gaaaacgctg agattcccac ctgttccttt cgtcaaatct
    34381 gtttctacta gcttcttacc atatttaatt aattcttgtt ttacatctga catccttgtc
    34441 ctcctcatat caaaacacta aatatttttc aattcaccaa ttatcccccc ttcctgcaca
    34501 aaagctaaga agggggaggg aggcaaattt acaaactctc aatcttttta attcttttgg
    34561 gagtaataac tctctaattg tagaaactcc tcaataacat ggtgagcctc ttccatccct
    34621 tttaattgtc ccatagctat gagctgcaca acaatattcc ccacagctgt agcctcagtc
    34681 aaacctgtaa gaacctcttt accagttcta tcagcaatca tttggttaaa gtaactggct
    34741 ctagcacctc ctccaatcac atatatcctc ttataaacct tatgagttaa ttcttctagt
    34801 aactctatcg ctttcctata cgtttcagct aggctttcat aaacaatctt aaatagttgt
    34861 ccatctgtcc actctctagt ttcatgatga taagctagat attctgtcaa agtcttgtgc
    34921 atatcagatt ctgttgcaaa ttcagttgat tcagtatcaa tcagaggaag attttctttt
    34981 tctttctcca ccattgtcct aatatcatca aaagaatagg ctttccctct tcgttcaaat
    35041 gaacgtctta gttcctctat gatccacaac cctgtacaat tcttcagaaa tgtaatcact
    35101 ccatctttac cgacttcatt tgtaaatcca taactgaagg attcggtagt aagaatcggt
    35161 gaagtaagtt ccactccaac caaagaccaa gtacctgatg aaataaataa actaccttct
    35221 gtcttaggta ctgagacaat cgcgcttgct gtatcatgac tacaaacatt cacaacagga
    35281 atatcgccta aaccatactc ctcttttatc cttccaagaa catttccctc tgaaacaatt
    35341 tcaggaagta aagatgaatc caattcaaat agttttaaga tattctgatt ccaattttga
    35401 ctcctaggat caaataattg agttgttgaa gcaatgcttt tttctgtagc aaacttacct
    35461 gtcaagagat aattaaacaa atctggcatt aaaagaatct tattggtctt atagaaagag
    35521 tcaggagatt cttgacgtgc cttaaagagt tgaaacaagg tatttatctc cataatctga
    35581 tttcctgtct ctgaatacag tttttctaat tcagtcattt cagatatttc ctttaacact
    35641 ccctttgttc tttcatcacg ataatgaaca ggttgtaata acagcttacc ttcattatca
    35701 atcagtccaa aatcaactcc ccatgtgtca ataccgatag ataaaatctt gtaactagta
    35761 ttagccaagc ggatactttc aagaatttta gctagtagaa agtcaatatc ccaagataaa
    35821 tgccctttta ctctaatagg tagattagaa aagcgattta tttcttccat tactagttta
    35881 ttttcagaaa ggtaaccaac gattgctctt ccagaagtcg cacctaaatc tattgctaaa
    35941 accgcttcca tttttcttct ctcttcgtcc ttgattaact ttattataat ccccacgttt
    36001 tattttttca atatgtgaac gcttacttga ataatgttca aatgttaacc caatattcat
    36061 aaaaataatt caaattcctc caatttgtgc tataatatag ttgaaatgat aaattaggag
    36121 gagcttatga taaaagatga acgtgtactt gaattgattg aaattatcaa aaagaaaaaa
    36181 agaattgccg taaaagagct ggcagaaatc actttctcca gcacaagtac cttacgtcgt
    36241 gatttaattt tcttagaaaa tcaaggtctt atcaaaagaa agcacggata cgtgaccctg
    36301 tcctctatga acacaattga actttctcat caaatacgtg aaggagaaag tactaggcaa
    36361 aaaagactaa tcgctagtct cgctaaagac tttattcggt ctggtatgtg tatctatcta
    36421 gattctagta ctactgtcta cgaactctgt ccctatcttt ctgaacttga taatttgatt
    36481 atttttacaa atggtttaca tactgcacaa accctatctg aaactgttaa agatagctcc
    36541 aaaatcttta tcacatctgg cgaggtcaaa catcaatcct gttccgtggt caactatgat
    36601 aaggaaaatt ctttattaga tcattttaat atcgatttag cattttgttc agcaagaggt
    36661 attgatgacc aatatgttta tgaagcttct ctcagccaag ctatttcaaa aaagaatatt
    36721 attgacaaag cccatgaaac catcttactg attgatagtt ctaaatttta caagactgga
    36781 ttttttaaaa ttaatcccct ctccaaatac acaaccttta tttcagacac cgtgccagat
    36841 caaaaattat tagacgcagt cgaattattt gatggagaat gggtttctga tattcaatga
    36901 aaatcaaata ggaagctagt ctcagcttgc tcaaagtaca gatttgaaaa ttgatcaagt
    36961 cagctcgaaa cactgttttg aggttgtaga tagaactgat gaagtcagtc acatatatac
    37021 ggaaagacga agctgacatg atgtgaagag attttcgaag agtatcgttc tgtctgctga
    37081 ctgatttaca aatattcacc acatgacatt attttcttag caaccctaaa aacagaggct
    37141 ggacaaaaac tcaatttcag gagaaaatgg agtaaacctt cccacaataa aacgcatagt
    37201 atcaagtttt tgcacacctg atattatgcg tttttctgat tttaaagatt tttctcttag
    37261 atctgatatt tcaagaatta agtgaccttg atgatctaaa tgtattgtgg tatactattt
    37321 ttgtcatcgg ttaccgatta cgttcctatg ggttcgtgag aggaggtgaa actaatgagc
    37381 cttatcatag agctcgctct tactattata gcggatgtta tagctggaat tatcttgtat
    37441 ttcgtctgca gatggctaga tagtaaggag tagaccgact gactagccta taaacacccg
    37501 ttaaatcgct aagatacgtc aaaaaagccc ttaactacgg ccatagttag gggctttggt
    37561 gttctaatga accttatcaa ttaactacat tctagcatat aatctacaat agttcaagca
    37621 ttaagtaagg ttgatgacct gaatgtagtg tgatatacta tttttgtcat cggttaccga
    37681 ttacgttcct tacggttcgt gagaggaggt gaaactaatg agccttatac cagagcttgc
    37741 tcttactatt atagcagatg ttatagctgg aattatcttg tatttcgtct gcaaatggct
    37801 agatggtaag aagtagaccg aatgactagc ctataaacac ccgttaaatc gctaagatac
    37861 gtcaaaaaag cccttaacta tggcactagt taggggcttt ggtgttctaa tgaaccttat
    37921 acactaacta cattctagca tataagccca gatatttcaa gagttttatt tattg
//
LOCUS       DAVHEX010000012        37236 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVHEX010000012 DAVHEX010000000
VERSION     DAVHEX010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37236
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.012"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(<1..326)
                     /locus_tag="VVL45_000609"
     CDS             complement(<1..326)
                     /locus_tag="VVL45_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU4186823.1"
                     /translation="MEQLHFITKLLDIKDPNIKILDIINMDTHKEIIAKLDYEAPSCP
                     DCGSLMKKYDFQKPSKIPYLETTGMPSRILLRKRRFKCYHCSKMMVAETPLVKKNHQI
                     PRIINQ"
     gene            complement(466..990)
                     /locus_tag="VVL45_000610"
     CDS             complement(466..990)
                     /locus_tag="VVL45_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEU4186824.1"
                     /translation="MKIAIIGYSGSGKSTIAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFSPLTCLFRAFKRYL
                     KYRGKVRESMAVDCPERFEWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(1043..1279)
                     /locus_tag="VVL45_000611"
     CDS             complement(1043..1279)
                     /locus_tag="VVL45_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEU4186825.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLVK"
     gene            complement(1347..2267)
                     /locus_tag="VVL45_000612"
     CDS             complement(1347..2267)
                     /locus_tag="VVL45_000612"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEU4186826.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEEKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWISINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            2457..2860
                     /locus_tag="VVL45_000613"
                     /pseudo
     CDS             2457..2860
                     /locus_tag="VVL45_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3076..3657
                     /locus_tag="VVL45_000614"
     CDS             3076..3657
                     /locus_tag="VVL45_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4186827.1"
                     /translation="MYYAENPDAAHDIHELRVDLLGEKMTFLTDAGVFSKKMVDFGSQ
                     LLLKCLDVNQGETVLDVGCGYGPLGLSLVKVYGVQATMVDINTRALDLAQRNAEKNNA
                     KATIFQSNIYARVQGRFDHVISNPPIRAGKQVVHEIIEKSKDFLEIGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            3654..4931
                     /locus_tag="VVL45_000615"
     CDS             3654..4931
                     /locus_tag="VVL45_000615"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEU4186828.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLIEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIKGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVHQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            4949..5611
                     /gene="deoC"
                     /locus_tag="VVL45_000616"
     CDS             4949..5611
                     /gene="deoC"
                     /locus_tag="VVL45_000616"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEU4186829.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYDFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGNL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKVVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVTLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            5598..5987
                     /locus_tag="VVL45_000617"
     CDS             5598..5987
                     /locus_tag="VVL45_000617"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU4186830.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMAEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            6077..7129
                     /locus_tag="VVL45_000618"
     CDS             6077..7129
                     /locus_tag="VVL45_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEU4186831.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALNNAVKDAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAERGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            7273..8808
                     /locus_tag="VVL45_000619"
     CDS             7273..8808
                     /locus_tag="VVL45_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4186832.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            8801..9859
                     /locus_tag="VVL45_000620"
     CDS             8801..9859
                     /locus_tag="VVL45_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4186833.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            9862..10818
                     /locus_tag="VVL45_000621"
     CDS             9862..10818
                     /locus_tag="VVL45_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4186834.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGVIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
     gene            10903..12174
                     /locus_tag="VVL45_000622"
                     /pseudo
     CDS             10903..12174
                     /locus_tag="VVL45_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(12217..12858)
                     /gene="plsY"
                     /locus_tag="VVL45_000623"
     CDS             complement(12217..12858)
                     /gene="plsY"
                     /locus_tag="VVL45_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEU4186835.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            12994..14937
                     /gene="parE"
                     /locus_tag="VVL45_000624"
     CDS             12994..14937
                     /gene="parE"
                     /locus_tag="VVL45_000624"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEU4186836.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSNWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGFKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAVVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVVNTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            15357..17828
                     /gene="parC"
                     /locus_tag="VVL45_000625"
     CDS             15357..17828
                     /gene="parC"
                     /locus_tag="VVL45_000625"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEU4186837.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKKTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAATRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            17958..18980
                     /locus_tag="VVL45_000626"
     CDS             17958..18980
                     /locus_tag="VVL45_000626"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEU4186838.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGTGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            19036..19740
                     /locus_tag="VVL45_000627"
     CDS             19036..19740
                     /locus_tag="VVL45_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU4186839.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     SRTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASHLNIIDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGSPVVSNVVPFSKPYSQVGIKGDPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKHIK"
     gene            19805..20494
                     /locus_tag="VVL45_000628"
     CDS             19805..20494
                     /locus_tag="VVL45_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEU4186840.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            20491..21414
                     /locus_tag="VVL45_000629"
     CDS             20491..21414
                     /locus_tag="VVL45_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEU4186841.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            21429..22073
                     /gene="pcp"
                     /locus_tag="VVL45_000630"
     CDS             21429..22073
                     /gene="pcp"
                     /locus_tag="VVL45_000630"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEU4186842.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            22241..22471
                     /locus_tag="VVL45_000631"
     CDS             22241..22471
                     /locus_tag="VVL45_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEU4186843.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            22521..22601
                     /locus_tag="VVL45_000632"
     tRNA            22521..22601
                     /locus_tag="VVL45_000632"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22555..22557,aa:Tyr,seq:gta)
     gene            22611..22682
                     /locus_tag="VVL45_000633"
     tRNA            22611..22682
                     /locus_tag="VVL45_000633"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22643..22645,aa:Gln,seq:ttg)
     gene            22844..24046
                     /gene="rpsA"
                     /locus_tag="VVL45_000634"
     CDS             22844..24046
                     /gene="rpsA"
                     /locus_tag="VVL45_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEU4186844.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFVDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(24132..25344)
                     /locus_tag="VVL45_000635"
                     /pseudo
     CDS             complement(24132..25344)
                     /locus_tag="VVL45_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            25538..26035
                     /locus_tag="VVL45_000636"
     CDS             25538..26035
                     /locus_tag="VVL45_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU4186845.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTAWDFTM
                     FEEKS"
     gene            26035..27690
                     /gene="dnaX"
                     /locus_tag="VVL45_000637"
     CDS             26035..27690
                     /gene="dnaX"
                     /locus_tag="VVL45_000637"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEU4186846.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNVGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            27718..27912
                     /locus_tag="VVL45_000638"
     CDS             27718..27912
                     /locus_tag="VVL45_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEU4186847.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            28062..28832
                     /gene="sufC"
                     /locus_tag="VVL45_000639"
     CDS             28062..28832
                     /gene="sufC"
                     /locus_tag="VVL45_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEU4186848.1"
                     /translation="MSVLEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            28860..30122
                     /gene="sufD"
                     /locus_tag="VVL45_000640"
     CDS             28860..30122
                     /gene="sufD"
                     /locus_tag="VVL45_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEU4186849.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIECVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIVGKNSKISYLERLESRGEGSDKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            30133..31359
                     /locus_tag="VVL45_000641"
     CDS             30133..31359
                     /locus_tag="VVL45_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU4186850.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQFASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            31346..31786
                     /locus_tag="VVL45_000642"
     CDS             31346..31786
                     /locus_tag="VVL45_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEU4186851.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQDQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            31840..33252
                     /gene="sufB"
                     /locus_tag="VVL45_000643"
     CDS             31840..33252
                     /gene="sufB"
                     /locus_tag="VVL45_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEU4186852.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFNQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(33451..34692)
                     /locus_tag="VVL45_000644"
     CDS             complement(33451..34692)
                     /locus_tag="VVL45_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEU4186853.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            34795..35904
                     /locus_tag="VVL45_000645"
     CDS             34795..35904
                     /locus_tag="VVL45_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU4186854.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
     gene            36550..36897
                     /gene="ssrA"
                     /locus_tag="VVL45_000646"
     tmRNA           36550..36897
                     /gene="ssrA"
                     /locus_tag="VVL45_000646"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(37080..37167)
                     /locus_tag="VVL45_000647"
     tRNA            complement(37080..37167)
                     /locus_tag="VVL45_000647"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37131..37133),aa:Ser,seq:gga)
BASE COUNT        11163 a         6608 c         8238 g        11227 t
ORIGIN      
        1 ttttggttga tgatacgagg gatttggtga tttttcttta ccaggggagt ctcagcaacc
       61 atcatttttg aacagtgata gcacttgaaa cggcgttttc taaggagaat tctagaaggc
      121 ataccagttg tttcgaggta agggatctta gacggttttt gaaagtcata tttcttcatt
      181 agacttccac aatcagggca agatggagcc tcataatcca gcttagcgat aatttctttg
      241 tgggtatcca tattgatgat atctagaatc ttgatgtttg ggtctttaat atcgagcagt
      301 tttgtgataa aatgtaattg ttccatatga ttctttctaa tgagttgttt tgtcgctttt
      361 cattataggt catatgggac tttttttcta cacaaaaata ggctccataa tatctatagt
      421 ggatttaccc actacaaata ttatagagcc caaaaaggaa gccctttatg aattgtagga
      481 cttccttttc ttatccagaa attgatctag ctctctctga tttcgaagaa tagtgacttt
      541 atgtgaatat tcttggcaaa gtttttggta attttctttt tgagttttgc tacgcccatc
      601 ccaaagaatc catctgataa actcccactc aaagcgttca gggcaatcta ccgccatact
      661 ttctctgact tttccacggt atttaagata acgcttaaag gctctaaaga gacaggtcaa
      721 tggcgaaaaa ttgagaaaga tgatttggtc agcttcttgc attcgttctt ggtagtagca
      781 ccaagaataa ttaccatcga tgacccaagc tttatgcttg gtgagaaagt tttttatctc
      841 ggttaacatc cattcgcagt cactgtcttg ccaaccaggt tgaaattgga gtgtgtccat
      901 gtgcagtttt ggaatggagt agtagttaga taacttttct gctatagttg acttaccaga
      961 accagaatat ccgataattg cgattttcat tttctacctt ttcctatttg gagacaaaaa
     1021 aacagcctct atggactgtt tcttatttaa caagtttagc tgaaagacga gctttatcgc
     1081 ggcttgcttt gtttttgtga atcaaacctt tagtttctgc tttatcgata gctgagctag
     1141 cagcacggaa aagttcttca gatgggtttg cttcgaaagc ttttatagca gtacgcatag
     1201 ctgatttttg agctgagttc ttttcgtttt gtttaacgtt caattcagcg cgtttgatag
     1261 ctgatttaat gtttgccaat gttcttacct ccatatttac taactatacc attatatatg
     1321 aaaacttacg ttttgacaag ggaaaattac ttttttaagt aaatttcatc gatttcatgg
     1381 ttcttgcttt tatgaagaat cacttctgca cgatttctgg ttggttcaat ataattttgc
     1441 agatttgtga gattgatact gatccagacc tgatgggcaa aggactccac ttccccaatc
     1501 ggcatctgag taaaacgata atagtagcta tcagggtcgt tttgggctag actcagcatt
     1561 ttcaagaaac ggtccagata ccaactttca atatcatcca ctccagcatc tacatagatg
     1621 gaaaagtcaa agaagtcagt gatatagaga cgatcgtttt gtggattttg aaagacattg
     1681 attccctcaa caattacaaa atcagcagct ttgacacttt gtttctcttc gggtacgatg
     1741 tcgtaaactt catgagaata gacaggaata tctacatctt gtccattttt gatgcggtcc
     1801 aagaagttga gaagagcttc catatcatag ctttcaggaa atcctttacg atttaaaatc
     1861 ccctgctcaa tcaaggtttg attgggatag agaaaaccat cagttgtaac caactcaacc
     1921 gtagcatctg taaacgtacg ggacagtagg atttgaagta ggcgactggt tgtggatttt
     1981 ccaacggcaa cactcccaga aaccccaata ataaaaggtt gagatttact ttcacgttgg
     2041 aggaaaattc cttttgaaaa ggctaaatct tccttagttc gcttgtaaat ctgaatcaaa
     2101 tgagccaagg ggagatagat atctgtaacg tcttggagac tgatttggtc attaaaactc
     2161 ttgatagatt ccaattcttc ttctgtcaaa ggaggtgttg tctttcgatg taaagattgc
     2221 caagtctggc ggctgatttt ttcaaaatgt aaaaattcgt tggtcatttg ttttccccta
     2281 gatattttgt ctatcatacc ataaaaagct aaaaaaataa agcggtttct tgaatgtggt
     2341 aagtcaatgg tatatagtat aagtgatcaa tttattgatg aaataggaga tagattctac
     2401 tgatttataa gcttataaag ggggcttttt agttcacaac ttataggagg tgtactatga
     2461 aaatcttaaa acgttacata ttggaactct gttttatttt aagttttgct ttacctttta
     2521 taaaaggaac caatgcagat aatggtagat gctttgtgga aacctattac ggttttactt
     2581 ttttgatgga acatgctatt gtaacagctg tctttatttg ttcgttctta attgctttct
     2641 tactaaaaaa cgatggacga aatggattgc tgcgggtagt tattgctttt tagtcctacg
     2701 gattgcgaca gagggttatt tcttccacat gtcactagaa gatttgatac gactttggac
     2761 aagtttggaa atcctgacac aaacgtatca gttgggcttt tatctaaaca ttttgttagg
     2821 aattttgttg ataataaagt attttaaggt taagcaatag aaataataat ctgcctgatt
     2881 atagatgata aactttttgt ttttatctgg actgtgatat ttatagttct aaatggttta
     2941 ttatttataa gtgttgaaag agattgaaga aaatggcttc gtgacttgct ttcttcggtc
     3001 tttttcgttt tattatcaat ttgtaaacga tttacaattt gagtcaaatt atggtaaaat
     3061 agtgtcatga gtaaaatgta ctatgcagaa aatcctgacg ctgctcacga cattcatgag
     3121 ttgagagtgg acttgttggg agaaaaaatg acttttttga cagatgcggg tgtttttagc
     3181 aagaaaatgg ttgactttgg aagtcaactc ttgctcaagt gtctggatgt caaccaagga
     3241 gagacagtcc tagatgtagg ctgtggttat ggtccattag gtttgtcctt ggtcaaggtt
     3301 tacggagttc aggcgaccat ggttgatatt aacactcgtg ccctggattt agctcagaga
     3361 aatgctgaaa aaaataatgc aaaagcgacg atatttcaat ctaatattta tgcaagagtt
     3421 caagggcgat ttgaccatgt catttccaat cctcctatcc gtgcgggcaa gcaagtcgtt
     3481 catgaaatca tcgagaaaag taaagatttc ttggaaattg gtggagattt gaccatcgtc
     3541 atccagaaaa aacaaggagc tccaagtgcc aagtctaaga tggaagatgt gtttggcaat
     3601 tgtgaaatcc ttaaaaaaga caagggatac tatatcctta gaagtgtgaa agaatgagag
     3661 cagttgattt aatccaaaaa aaacgagacg gtcaagaact gacttcaagt gaaattgaat
     3721 ggctgataga aggctatgta tcaggaactg ttcctgatta tcagatgtct gcctttgcta
     3781 tggctgttta ttttaaagga atgacgactc gagaaatctc tgatttaacg atgaatatgg
     3841 ttaagactgg gcaagagttt gacctgtcag ccattgatgg tgtgaaagtt gacaagcatt
     3901 ctactggtgg tgtcggcgat aaggtaacct tgattttagc tcctcttgtt gctagcttcg
     3961 gtgtgcctgt agcaaaaatg agtggtcgtg gtcttggcca tacaggcgga acaattgata
     4021 aattggagtc cattaagggc tatcaagtag aacgtagtca agaggatttt attcgtcagg
     4081 ttcaggatat tggagtatct gtcattgggc aatcagacca gctggtcaaa gcagataagc
     4141 ttctctatgc ccttcgtgat gtgaccgcaa ctgtcgacac gattcctttg attgcgagtt
     4201 cggtgatgag caagaaaatt gcggcagggg cggatgctat tttgctagac gtgactgtcg
     4261 gtgagggtgc cttcatgaag acggttgatg aggcgcgtga gttggctcaa actatggtag
     4321 atcttggtaa ggttgttgga cggaagacag tagcagtgat tacggatatg agccagcctt
     4381 tgggacgagc gattggaaat cgtctggaaa tccttgaagc attggagatt ttgcaaggtc
     4441 aaggacgtca ggatattacc cactttattt gtgaattggc tcaaattatg cttggtttgg
     4501 caaatgttaa caaaactgtt gaagaagttc accaacatct tgagaatggt caagcactgg
     4561 ctaagtttga ggaaatggtg caagcccaag gtggagactt ggaagacctc tatcgtcctg
     4621 taaatgtagc tcatgtggtg gaaatccctg ctcaggaaac gggtgtcatt tcagctcttc
     4681 cagctatgga ctttggtctt tatgccatga ggctgggtgc tggtcgtgca gttaagtctg
     4741 atgccttgga ctatgaaaca ggaattgttt ttgaaaagaa gattggagac tccgttcaaa
     4801 agggtgaaat tgttgcaaaa gtatacacaa atgaaaaaat tccacctcaa ctagttacag
     4861 atttccaaaa atgtgttaaa ataagtgatg aagtgaaaaa aatccgtgaa attgtagaaa
     4921 tcatctccta aatggaggga aaaccgaaat gaaattaaat aaatatatag atcatacgct
     4981 tttaaaacaa gatgcaaaga aaaaacaaat tgatagtttg ttgtctgagg ctagagagta
     5041 tgactttgcc agtgtttgcg ttaatccgac ctgggttgaa catgctaaaa aaggacttga
     5101 aggcacagat gttaaggttt gcacagtagt aggtttccct ttgggagcaa caacttcagc
     5161 cgtgaaagca tttgagacaa aagaagctat ccaaaatggt gcagatgaga ttgatatggt
     5221 gatcaatgtt ggagctctca aatcaggtaa tttagccttg gttgagtcag atattcgcgc
     5281 agtagtggaa gcaagtggtg ataagttagt gaaagtcatt attgaagctt gccttctgac
     5341 agaccaagaa aaagttgttg tttgccaatt ggcccaaaaa gctggggctg actttgtcaa
     5401 aacatctact ggcttttcaa ctggtggtgc tacgatagca gatgttacat taatgcgtga
     5461 aacagttgga tctgatatgg gtgtcaaggc cgccggtgga gctcgttctt atgcagatgc
     5521 tcttgccttt gtcgaagcag gtgcgacccg tatcggaacg tcagctgggg tagctatttt
     5581 aaaaggagaa ttggcagatg gcgactactg agttgattga actggcaatt gaaaccagca
     5641 aacatgccta tgtcccctat tctcactttc ctatcggagc agttttagta gctaaagacg
     5701 gaagtgttta cacgggagtg aatattgaaa atgctagcta tcctttgacc aattgcggtg
     5761 agagaacagc tatttttaag gctatatctg aaggccaaag agaattttca gaattgattg
     5821 tctatggtca aactgaaaaa ccgatctcgc catgtggtgc ttgtcgccaa gtgatggctg
     5881 aattttttga acaagatcta aaagtgacct tagtcgcaaa agataaatcg acggtcgaga
     5941 tgacggtcgg ggaattactt ccatactctt ttacagactt aaactagtct gagtcgctct
     6001 ctgagtggca cggtccttgt ggccaaccaa tccatacttg caacatcgtt gcacatctta
     6061 tttaggaggt tcagtaatga acaagaaaca atggctaggt cttggcctag ttgcagtggc
     6121 agcagttgga cttgctgcat gtggtaaccg ctcttctcgt aacgcagctt catcttctga
     6181 tgtgaagaca aaagcagcaa tcgtcactga tactggtggt gttgatgaca aatcattcaa
     6241 ccaatcagct tgggaaggtt tgcaggcttg gggtaaagaa cacaatcttt caaaagataa
     6301 cggtttcact tacttccaat caacaagtga agctgactac gctaacaact tgcaacaagc
     6361 ggctggaagt tacaacctaa tcttcggtgt tggttttgcc cttaataatg cagttaaaga
     6421 tgcagcaaaa gaacacactg acttgaacta tgtcttgatt gatgatgtga ttaaagacca
     6481 aaagaatgtt gcgagcgtaa ctttcgctga taatgagtca ggttaccttg caggtgtggc
     6541 tgcagcaaaa acaactaaga caaaacaagt tggttttgta ggtggtatcg aatctgaagt
     6601 tatctctcgt tttgaagcag gattcaaggc tggtgttgcg tcagtagacc catctatcaa
     6661 agtccaagtt gactacgctg gttcatttgg tgatgcggct aaaggtaaaa caattgcagc
     6721 cgcacaatac gcagccggtg cagatattgt ttaccaagta gctggtggta caggtgcagg
     6781 tgtctttgca gaggcaaaat ctctcaacga aagccgtcct gaaaatgaaa aagtttgggt
     6841 tatcggtgtt gatcgtgacc aagaagcaga aggtaaatac acttctaaag atggcaaaga
     6901 atcaaacttt gttcttgtat ctactttgaa acaagttggt acaactgtaa aagatatttc
     6961 taacaaggca gaaagaggag aattccctgg cggtcaagtg atcgtttact cattgaagga
     7021 taaaggggtt gacttggcag taacaaacct ttcagaagaa ggtaaaaaag ctgtcgaaga
     7081 tgcaaaagct aaaatccttg atggaagcgt aaaagttcct gaaaaataat ggatggaagt
     7141 catttcttag gaagcggcca agggccgctt ttttaagagt tgagacaaag tcattttgtc
     7201 taagtaaagc ttgctactag acaaactagc aagttttatt gaaataaaat aagactctga
     7261 aaggaagagc acatggcaca cgaaaatgtc attgagatgc gtgatattac caaggtgttt
     7321 ggtggatttg ttgccaacga caaaatcaac ttgcacctac gaaaaggtga aattcatgca
     7381 cttttaggag aaaatggggc tggtaagtcc acgctaatga acatgttagc aggccttctt
     7441 gaaccaacta gtggtgaaat cgcggtcaac ggtcaagttg tcaatctcga ctccccatct
     7501 aaagcagcta gcttgggaat cgggatggtt caccagcact ttatgttggt tgaagccttc
     7561 acagtggctg aaaacatcat tttaggtagt gaattgacta aaaatggtgt gctagatatc
     7621 gctggagcta gcaaagaaat caaggctctt tctgaacgtt atggcttagc tgttgaccct
     7681 tctgccaagg tagcagatat ctcagttgga gcccaacaac gtgtagaaat tttaaaaaca
     7741 ctttatcggg gggctgatat ccttatcttt gacgaaccaa cggctgtttt gactccatca
     7801 gaaattgatg agttgatggc tattatgaaa aatcttgtca aagaaggaaa atcaattatc
     7861 ttgattaccc acaaattgga tgaaattcga gcagtttctg accgtgttac agttatccgt
     7921 cgtgggaaat caattgaaac cgttgaaatt gcaggggcta ccaatgctga tttggcggaa
     7981 atgatggtag gacgttctgt ttcctttaaa acagagaagc aagcctctaa accaaaagaa
     8041 gtggttttgt ctatcaaaga tttggtggtc aatgaaaacc gtggtgttcc agctgttaaa
     8101 aatctgtcct tggatgttcg tgctggagag attgttggta ttgcggggat tgatggaaat
     8161 ggtcagtctg aactgattca agccattaca ggtcttcgta aggttgaatc tggtagcatt
     8221 gagctaaaag gagattcaat tgtaggcttg cacccacgtc agattacaga actaagtgtt
     8281 gggcacgttc cagaagaccg tcaccgtgat ggcttgattt tggaaatgat gatatctgaa
     8341 aatattgccc ttcaaaccta ctataaagaa ccacatagta aaaatggaat tttgaattat
     8401 tcaaatatta cttcttatgc taaaaagctg atggaagagt ttgatgttcg cgctgccagt
     8461 gaattagttc ctgcagctgc actctcagga ggaaatcaac aaaaagcaat tattgctcgt
     8521 gaaattgatc gagatcctga tctccttatc gttagccagc caactcgtgg gttggatgtc
     8581 ggtgccattg agtatatcca caaacgcttg attgaagagc gtgataatgg caaggctgtc
     8641 cttgttgtca gctttgaatt ggatgagatt ttaaacgtct cagaccgtat tgccgttatc
     8701 cacgatggta agattcaagg tattgtatca ccagaaacaa ccaataaaca agaacttggt
     8761 gtcttgatgg ctggtggaaa cttgggaaag gagaagagtg atgtctaaaa aattacaaca
     8821 aatttcggtt cccttgattt ctgtattcct aggaatttta ctcggagcca ttgtcatgtg
     8881 gatcttcggt tatgatgcta tttggggcta cgaagaattg ttctatacag cctttggcag
     8941 tctgcgtggg attggagaaa tcttccgtgc tatgggtcct ctggtcttga ttggtcttgg
     9001 ttttgccgtt gccagtcgag ctggtttctt taacgtcgga cttcctggtc aggctttggc
     9061 aggttggatt ctcagtggtt ggtttgccct gtcgcatcca gatatgcccc gtcccttgat
     9121 gattctagca accatcgtga ttgccttgat tgctggtggg attgtcggag cgattccagg
     9181 tattcttagg gcctatctag ggacgtcaga ggttattgta accatcatga tgaactacat
     9241 tgtcttgtat gtagggaatg cctttatcca tgctttccct aaagacttca tgcaaagtac
     9301 agattcgacc attcgtgttg gggctaatgc aacctatcag acaccttggt tggctgagtt
     9361 gactggtaac tcacggatga atattggtat tttctttgcc atcattgccg ttgcagttat
     9421 ttggttcatg ctcaagaaaa caactcttgg ttttgaaatc cgtgcagttg gtcttaatcc
     9481 acatgcttca gaatatgctg gtatttctgc caagcggact attatcctat ctatgattat
     9541 ttcaggtgcc ttggcaggtc ttggtggagc tgttgaaggt ttgggaacct tccagaacgt
     9601 ctatgttcaa ggttcgtcat tagctatcgg atttaacgga atggcggtta gtttgcttgc
     9661 ggccaactca ccaattggta tactctttgc agccttccta tttggcgttc tccaagttgg
     9721 ggctcctggt atgaatgcgg cgcaggtacc atctgagctt gtcagcattg taacagcgtc
     9781 tattatcttc tttgtcagtg ttcattacct tatcgaacgc tttgtcaaac cgaaaaaaca
     9841 agttaaagga ggtaagtaaa gatgtctatt ataaccttgc tccccctctt ggtgtcttct
     9901 atgctgattt actcagcacc tctcatcttt acaagtatcg gtggtgtttt ctctgaacgt
     9961 ggtggtgtgg taaacgttgg ccttgaagga attatggtta tgggtgcctt ttctggagtt
    10021 gtctttaacc ttgaatttgc agaacaattt ggagcagcaa ctccatggct atccttgctt
    10081 gtagcaggat tggttggtag tgttttctct atcatccacg cagcagcgac ggttcatttc
    10141 cgtgcagacc atgttgtcag cggtacggta ttgaacttga tggcgcctgc cttggctgtt
    10201 ttcttagtta aagttcttta taacaaagga caaaccgaca acctaagtca aacttttgga
    10261 cgctttgatt tcccagtctt ggcaaatatc ccagtgattg gtgatatctt cttcaagtca
    10321 actagtctac ttggttatct ggcgattgcc ttctcattcc ttgcttggtt tattctcttt
    10381 aagactcaat ttggtcttcg tctccgctct gtcggtgaac atcctcaagc agcggatacc
    10441 ttgggaatca acgtctacaa gatgagatat ttaggggtta ttatttcagg ttttctaggt
    10501 ggaattggcg gagcgattta tgcgcaatca atctcagtta acttctcagt gacaactatt
    10561 gttggacctg gatttatcgc ccttgctgcg atgatctttg ggaaatggaa tccaatcggt
    10621 gctatgcttt ctagtctctt ctttggactt tcacaaagtt tggctgttat cggttctcaa
    10681 ttgccgttcc tacaaggagt gccagcggtt tatcttcaaa ttgcacctta tgttttgaca
    10741 attcttgtct tagcagcctt ctttggaaaa gcagtcgcac caaaagcaga tggtatcaac
    10801 tacatcaaat caaaataagc atacaaaaaa acgtcagtcc aatgactgaa ctacacccca
    10861 aaagttagac agaaaaaatc taacttttgg ggtgttttta ttatgaaatt aagttatgat
    10921 gataaagttc agatctatga acttagaaaa caaggatata gcttagagaa gctttcaaat
    10981 aaatttggga taaacaattc taatcttagg tatatgatta aattgattga tcgttacgga
    11041 atagagttcg tcaaaaaagg aaaaaatcgt tactattctc ctgatttaaa acaagaaatg
    11101 attaataaag tctgacatga aggctggact aaagatagag tttctcttga atacggtctc
    11161 ccaagtcgta cgatacttct taactggcta gcacaataca ggaaaaacgg gtatactatt
    11221 gttgagaaac caagagggag agtacctgag agcggagaat gccatcctaa aaaagttaag
    11281 agaactccga ttgaaggagg aaaaagagaa agaagaaaga cagaaattgt ttaagaatta
    11341 atgactgagt tttcgttaga tcttctttta aaagtcatta aactagctcg ttcgacctac
    11401 tactatcact tgaaacagct agataaacca gataaggacc aagagcttaa agctgaaatt
    11461 caatccattt ttatcgaaca caaaggaaat tatgcttatc gtcggattta tttagaacta
    11521 agaaatcgtg gttatctggt aaatcataaa agagttcaag gcttgatgaa agtactcaat
    11581 ttacaagcta aaacgcgaca gaaacgaaaa tattcttctc ataaaggaga cgttggcaag
    11641 aaggcagaga atctcattca aggccaattt gaaggctcta aaacaatgga acagtgctac
    11701 acagatgtga ctgaatttgc cattccagta agtacttaaa agctttactt atcaccagtt
    11761 ttagatggct ttaatagcga aattatcgcc tataatcttt caacttcacc caacttagaa
    11821 caagtacaaa caatgttgga acaggcattc acagagaagc actacgagaa tacgattctc
    11881 catagtgacc aaggctggca ataccaacac gattcttatc atcggttcct agagagtaag
    11941 ggaattcaag catccatgtc acgtaagggc aacagccaag acaacggtag gatggaatct
    12001 ttctttggca ttttaaaatc cgaaatgttt tatggctatg agaaaacatt taaatcactt
    12061 aatcaattgg aacaagccat tatagactat attgattact acaacaacaa acgaattaag
    12121 gtaaaactaa aaggacttag tcctgtgcag tacagaacta aatcctttgg ataaattatt
    12181 tgtctaactg tttgggggca gtacagaact ggcattttat tttttaggat cttgatgggt
    12241 taggttcaat ccccaaggga ccaaattttc agttttattt ttgatacgag ctatattgtc
    12301 cttatgacga atgataatca aactagcaag tgctaagata atagcgatga agagagagtc
    12361 atagttactc aggataaaac caaaaagtgg aaagagcaga accccgataa cagccgcaat
    12421 cgatgctgtg acactagaca gtgaaatcat actgccaaga tagagagctc caaagaagat
    12481 aatcgcaagg tagagacaga agataggcgc aaatccgaaa atcactccag cactggttgc
    12541 gacagcctta ccacctttaa atcctgcaaa gatagggaag gtatggccga taacagccaa
    12601 aagtccaaag atgagaggag aaacgccttg tagatgaaaa ataatcggaa gcagcgttgc
    12661 tagggttcct ttgaaaaagt caatcacaaa ggttgccata ccagctttct tacctaaaat
    12721 gcggaaggta ttggtcgttc cagtgttacc agaaccatgc tcgcgtagat tgatttgaaa
    12781 gaatacttgt ccaatccaga gaccagatgg aatcgaaccc agcagatagg ctaggattaa
    12841 taaaactatt gtaatcatac tcctattata tcatgaattg ggaaagaaaa gcagagaatc
    12901 tctgctgaaa ttgtcacatc ggagaaagaa aaattttgca aaatccttgg aaaacctgta
    12961 gaatagtaaa gatgaacgaa taggaggttc cttgtgtcaa aaaaggaaat caatattaac
    13021 aattataatg atgatgctat tcaggtgcta gaagggttgg atgcggtccg aaaacgtcca
    13081 gggatgtata ttggatcgac cgatggcgct ggtcttcatc acctagtttg ggaaatcgtt
    13141 gataatgcag tcgatgaagc cttgtctggg tttggtgatc gtattgatgt aactatcaat
    13201 aaagacggta gtctaacggt tcaagaccat ggacgtggga tgccgacagg tatgcacgct
    13261 atgggaattc caactgttga ggttatcttt accattcttc atgccggagg gaaattcggt
    13321 caaggtggct ataagacatc aggtggactt cacggagtgg gttcttccgt tgttaacgcc
    13381 ctttctaact ggttagaagt tgaaattacc cgtgatggtg cagtttacaa gcaacgtttc
    13441 gaaaatggtg gaaaacctgt cacgactttg aagaaaatcg gtacagcacc caagtctaaa
    13501 acaggcacca aagttacttt tatgcctgac gcgactatct tttctacgac agatttcaag
    13561 tacaatacca tttcagagcg ccttaatgaa tcagcctttc tcttgaaaaa tgtgaccttg
    13621 tctttaacgg acaagcgaac agatgaagcg attgagttcc actatgagaa tggagtacaa
    13681 gattttgttt cttatctcaa cgaagataag gaaatcttga cgccagttct ttactttgaa
    13741 ggggaagaca atggttttca agtggaagta gccctccagt acaatgacgg attctcagat
    13801 aacattctat cctttgtcaa taacgttcgc accaaggacg gtggaacgca cgagacagga
    13861 ttcaagtctg ccattaccaa ggtcatgaat gactatgcac gtaaaacagg tcttctcaag
    13921 gaaaaagata aaaaccttga aggttcagac tatcgtgagg gactagcggt cgttctttct
    13981 atcttagttc ctgaagaaca cttgcagttt gaaggacaga ccaaggataa actaggaagc
    14041 cccctagctc gcccagttgt ggatggaata gtggctgata agttgacctt tttccttatg
    14101 gaaaatgggg aattagcttc taacctcatc cgcaaggcta tcaaggcccg tgatgctcgt
    14161 gaagcagcac gtaaggcgcg tgatgagagc cgaaatggga agaaaaacaa gaaagataag
    14221 ggcttgttgt ctgggaaatt gaccccagcc caatctaaga atcctgctaa gaatgaactc
    14281 tatctagttg agggggactc tgccggtggt tctgccaaac aaggtcgtga ccgcaagttc
    14341 caggctattc tacctcttcg tggtaaggtt gtcaatacag ccaaggccaa gatggcggat
    14401 atcctcaaaa atgaagagat taataccatg atttatacca ttggtgcggg tgttggagca
    14461 gacttctcta ttgaagatgc caactatgat aagatcatta tcatgaccga tgcggatacc
    14521 gacggtgccc atatccagac cttgctcttg acatttttct accgttacat gcgtccgcta
    14581 gtcgaggcag gtcatgtcta tattgccctc ccacctcttt ataagatgtc caaaggtaaa
    14641 ggcaagaaag aagaagtggc ctacgcttgg acggacggag aactagaaga actccgtaaa
    14701 cagttcggta aaggcgctac cctccaacga tacaaaggac ttggtgagat gaatgcggac
    14761 cagctctggg aaacaaccat gaacccagaa acacgtaccc tcatccgtgt cacaattgaa
    14821 gatttagcgc gcgccgaacg ccgcgtcaat gttctcatgg gagataaggt agaaccacgc
    14881 cgtaaatgga ttgaagataa tgtcaagttt acgctagaag aagcgacagt gttttaatga
    14941 aagaaaataa tatgttagta aactggaaat tagagtcgga tgtgaatgac tatgtgaaaa
    15001 agcagtttga aaatcttggt ttgaaaaaat tgcaggatta ttaatgaaga atctgctatg
    15061 agtgcttatc tcaaagaggc gcttaaggga gcggttaaga cacaaactaa gactaatttt
    15121 ggaaaaccag attttcatat tgaaaaatac aaacaggata gtttggttac ttatcgtaac
    15181 tgaacacggg aggaattccc gtaaaatatc ttttactgca taaaaaccta ctctacattc
    15241 tttgaaagga gttgaacacg ccctagatac tgtgtgaaaa agataaattc tcttgtgagt
    15301 ttgcttactt caagaatttt ctattttcac ttggtatttt atgggctttg tatcttatgt
    15361 ctaacattca aaacatgtcc ctggaggaca tcatgggaga gcgctttggt cgctactcca
    15421 agtacattat tcaagaccgg gctttgccag atattcgtga tgggttgaag ccggttcagc
    15481 gccgtattct ttattctatg aataaggata gcaatacttt tgacaagagc taccgtaagt
    15541 cggccaagtc agtcgggaac atcatgggga atttccaccc acacggggat tcttctatct
    15601 atgatgccat ggttcgtatg tcacagaact ggaaaaatcg tgagattcta gttgaaatgc
    15661 acggtaataa cggttctatg gatggagatc ctcctgcggc tatgcgttat actgaggcac
    15721 gtttgtctga aattgcaggc taccttcttc aggatatcga gaaaaagaca gttccttttg
    15781 catggaactt tgacgatacg gagaaagaac caacggtctt gccagcagcc tttccaaacc
    15841 tcttggtcaa tggttcgact gggatttcgg ctggttatgc cacagacatt cctccccata
    15901 atttagctga ggtcatagat gctgcagttt acatgattga ccacccaact gcaaagattg
    15961 ataaactcat ggaattcttg cctggaccag acttccctac aggggctatt attcagggtc
    16021 gtgatgaaat caagaaagct tatgagactg ggaaagggcg cgtggttgtt cgttccaaga
    16081 ctgaaattga aaagctaaaa ggtggtaagg aacaaatcgt tattactgag attccttatg
    16141 aaatcaataa ggccaatcta gtcaagaaaa tcgatgatgt tcgtgttaat aacaaggtag
    16201 ctgggattgc tgaggttcgt gatgagtctg accgtgatgg tcttcgtatc gctatcgaac
    16261 ttaagaaaga cgctaatact gagcttgttc tcaactactt atttaagtac accgacctac
    16321 aaatcaacta caactttaat atggtggcga ttgacaattt cacacctcgt caggttggga
    16381 ttgttccaat cctgtctagc tacatcgctc accgtcgaga agtgattttg gcgcgttcac
    16441 gctttgacaa agaaaaggct gagaaacgtc tccatatcgt cgaaggtttg attcgtgtga
    16501 tttcgatttt ggatgaagtc attgctctta tccgtgcttc tgagaataag gcggacgcca
    16561 aggaaaacct caaagttagc tatgatttta cggaagaaca ggctgaggct atcgtaactt
    16621 tgcaactgta ccgtttgacc aataccgatg tggttgtctt gcaggaagaa gaagcagagc
    16681 ttcgtgagaa gattgctatg ctggcggcta ttattggtga tgaaaggact atgtacaatc
    16741 tcatgaagaa agaacttcgt gaggtcaaga agaaatttgc aactcctcgt ttgagttctt
    16801 tagaagacac tgcgaaagca attgagattg atacagctag tcttatcgct gaggaagata
    16861 cctacgtcag cgtgaccaag gcaggttaca tcaagcgtac cagtccacgt tcctttgcgg
    16921 cttccacctt ggaagaaatt ggcaagcgtg atgatgaccg tttgattttt gttcaatctg
    16981 ccaagacaac ccagcacctc ttgatgttca caagtcttgg aaatgtcatc tacagaccaa
    17041 tccatgagtt ggcagatatt cgttggaagg acatcggaga gcatctgagc caaaccatca
    17101 caaactttga aacgaatgaa gaaatccttt atgtggaagt actggatcag tttgacgatg
    17161 cgacaaccta ctttgcagcg actcgccttg gtcaaatcaa acgggtagag cgaaaagaat
    17221 tcactccatg gcggacctat agatctaagt ctgtcaagta tgctaagctc aaagacgata
    17281 cagatcagat tgtagcagtg gctccgatta aactagatga tgttgtcttg gttagtcaaa
    17341 atggttatgc cctgcgtttc aatatcgaag aggttccggt tgtcggtgct aaggcagcag
    17401 gtgtcaaggc tatgaatttg aaagaagatg atgtcctcca atctggcttt atctgtaata
    17461 cttcgtcctt ctacctcttg acccagcgtg gaagcttgaa acgtgtttcc attgaggaaa
    17521 ttctagcaac cagccgtgcc aaacgaggat tacaagtctt gcgtgagttg aaaaacaaac
    17581 cgcatcgtgt cttcttggca ggagcagttg cagagcaagg atttgttggc gatttcttca
    17641 gtacggaagt ggatgtgaac gaccaaactc tgcttgtcca atccaataaa ggaacaatct
    17701 atgaaagccg attgcaagac ttgaacttgt cagaacgcac tagcaatgga agcttcattt
    17761 ctgacacgat ttcagatgaa gaagtttttg acgcttatct tcaggaagta gttactgaag
    17821 ataaataagt gaaatgaaga atcagttcta agatggtttt tattggagga attttctgaa
    17881 aattacaaaa tatacttgct attttagaaa aatagtgtag aatataagaa ataggttttt
    17941 agaataagga gtggaatatg acagtaacga ttgattggga aaacctcggt ttttcctata
    18001 tgaaattacc ttatcgctat cttgctcatt tcaaaaatgg acaatgggat caaggagagc
    18061 ttacagagga tgcaactttg catatttcag agtcttctcc aagtcttcac tatggacaac
    18121 aagcatttga aggtttgaaa gcttatcgta ctaaggatgg cagtgttcaa ctgttccgtc
    18181 ctgatgaaaa tgctaaacgt ctgcaacgta catgtgaccg tctcttgatg ccacaagttc
    18241 cgacagacat gtttgtagaa gcttgtaaag cagttgtccg tgcgaatgaa gaatacgtac
    18301 caccatacgg aacaggtgga accttatatc ttcgccctct tttgattggt gtcggagata
    18361 ttatcggggt aaaaccggca gaagagtaca ttttcaccat ctttgctatg ccagttggaa
    18421 attactttaa aggtggtttg gtcccaacca acttcttgat tcaggatgag tacgaccgtg
    18481 cagcaccaaa tggtacaggt gcggctaagg ttggtggaaa ctatgctgca agtctcttac
    18541 caggaaaaat ggccaagtca cgtcatttct cagatgttat ctatctggac ccatcaactc
    18601 atacaaagat tgaagaagtc ggatcagcta acttctttgg aattacagct gataatgaat
    18661 ttgtaacacc attgagtcca tctatcttgc catctattac caagtattcc ttgctttatt
    18721 tggcagaaca tcgcttggga ttaactccta ttgagggtga tgttccaatt gataatcttg
    18781 accgttttgt agaggcaggt gcctgtggta cagcagcggt tatttctcca attggaggta
    18841 ttcaacatgg tgatgatttc catgtattct atagtgaaac agaagtaggt cctgtgacgc
    18901 gtaaattata taatgaattg acgggtattc agtttggcga tattgaagcg ccagaaggtt
    18961 ggattgtaaa agtagattaa aataaaccaa aggagattct ttatgaaata gaaaaagtgg
    19021 ctcttaacag caggagtggt cctgagcacg tcagctattt tagtggcttg tggaaaaact
    19081 gataaagaag cagatgcacc gacaacattt tcttatgtct atgcagtaga tccagcatca
    19141 ttgggctaca gtatagcgac tcgaacatcg aggacagacg ttattggaaa tgttattgat
    19201 ggtttgatgg aaaatgataa atacggcaat gttgctcctt ctcaaaaaga ctatgatttg
    19261 aacagtacag gatgggctcc aagctatcaa gatccagcgt ctcacttgaa tattatagat
    19321 ccaaaatctg gttctgccat gaaacacctt ggtattacga aaggaaaaga taaggatgtt
    19381 gtagctaaac ctggtttgga taaatataag aaattgttag aagatgctgt ttctgagacc
    19441 actgacctag agaaaagata tgaaaaatat gccaaagctc aagcttggtc gacagatagt
    19501 tcattattga tgccaacagc ttcatctggt ggttctccag ttgtaagtaa cgtagtacca
    19561 ttctcaaaac catactcaca agttggtatt aagggggatc catatatctt taaaggaatg
    19621 aaattgcaaa aagatattgt tacaacaaaa gaatataacg aggtttttaa aaaatggcaa
    19681 aaagaaaaat tggaatccaa tagcaaatac caaaaagaac tagaaaaaca cattaaataa
    19741 ggaatggtat tgatcttgat aaaattttaa aaatactgtc attttgaata taaaggagtt
    19801 tgatatggag tggattagat taataggaat agcaatcatt gttgtgggtt ttattttaaa
    19861 atttgataca attgcaacag tagtcttagc tggtttggtt acagctttag tttcaggtgt
    19921 ttctctcgtt gaatttttgg agattttggg aaaagaattt agcaatcagc gagtgctcac
    19981 gatttttatg gttaccttgc ctcttgtggg gctgtcagaa acctttggac tcaagcaacg
    20041 atcaatcgat ttgattcgaa agattaaagg tctgacagtt ggaaacttct atacagttta
    20101 tttctttatt cgagagttag ctggtttctt ttcaattcgt ctaggaggac accctcagtt
    20161 tgtcagacct ttggttcaac ctatgggaga agcagctgca gagtctcaat taggtagaaa
    20221 gttaacagag gttgaagatg agacaataaa agcgcgtgcg gctgcgaatg aaaattttgg
    20281 aaatttcttt gctcaaaata cgtttgtagg tgctggggga gtcctcttga tagggggaac
    20341 attagatcag ttaggctatg aaagcaatta tgcagggatt gcttctacat ctattattgt
    20401 tgctgttata acacttattg tagtggggat ttataattat ttatttgata aaaaattgat
    20461 atcaaaaaag actaggggag gagaacaaaa atgacagagt tggcaaagca actattagag
    20521 ttgacctata ttgtgattgg ttgtcaattt ctccatacag cctattgtag ttataaagat
    20581 aaaacaaacc cagttcgact tgggacatct gcattttgga ctctattgtc tattacgttt
    20641 ataggtggtt cctatatgcc aaatatgagt attggtatta ttgtaatcct attatcgctg
    20701 ttaacattgt ttaagcaagt ccgtatcgga accttgccat ccttagatga aatgaaagcc
    20761 aatattgaat ctaacaggtt gaaaaataaa atttttattc cagttatgct gatggcaata
    20821 cttgcgttgg tcttagcgca aatgattcca gaatttagca agatttcgat tagccttgcc
    20881 gccttgtttg ctacaatttc tgttcttgtg attaccaata gtcaccctaa gagtctgtta
    20941 tcagaaaata atcgaatgac tcagcaagtt tcaacaagtg ggattgttcc tcaattatta
    21001 ggggctttgg gggctatttt tactgtagca ggtgttggtg atgttatctc tcatctgatt
    21061 agcggtattg ttccttcaga tagtcgcttt ataggagttt tggcctatgt tcttggaatg
    21121 gttctattca caatgattat gggaaatgct tttgcagcat tcaccgttat tacagcaggt
    21181 gttggagttc cctttgtatt tgctctggga gctaatccaa ttgtggctgg tgctcttgcc
    21241 atgacagcag gttattgtgg gaccttattg accccaatgg ctgctaattt taacgctcta
    21301 ccagcagcat tgatggatat gaaagatcag aatggcgtta taaaggctca agcaggtgtt
    21361 gctctagtaa tgattgttat tcacatattc ttaatgtact ttctcgcatt ttagtaaagg
    21421 aaaataaaat gaaaatattg gttacaggtt ttaatccttt tggaggtgaa aagattaatc
    21481 cagctttgga ggctgtaaaa ttattaccat ctgagattaa tggggctgaa gttcgctggg
    21541 tagaaattcc aacggttttt tataagtcgt cagaagtttt agaggcagaa atattacgat
    21601 atcaaccaga tgctgtactt tgtattggac aagcaggcgg caggaccggc ttaacacctg
    21661 aacgagtggc tattaatcaa gatgatgctc gcatacctga taacgaaggc aatcaaccaa
    21721 ttgatacacc gattcgtatt gatggagcat cggcctattt tagtagttta cctatcaaag
    21781 cgatggtaca agctatcaaa aaacagggac ttccggcagt tgtatccaat agtgcaggaa
    21841 cctttgtttg caatcatttg atgtaccaag ctctttattt agtggataaa aaattcccta
    21901 atatgagagc aggtttcatg catattccat atatgatgga acaagtagta aataagccga
    21961 atactgcagg aatgagtcta tgtgatattg taagaggcat agaggtagct attgaagcta
    22021 ttgtagacta caaagataag gatttgcagt tagtaggcgg tgagactcac tgataaccta
    22081 aaaaggatag tcaattatgc ttgtttacta actattaact atgctaaatc aattgaggtt
    22141 gtttacataa aactctatat cagagaagcc tgatatagag ttttttcttg ctagttttag
    22201 gatttttttg taaaatagaa aaagtgaaga gaggtatgaa atgagcaaga aagataaaaa
    22261 aatcgaaatt caagtagcgg atgccaaagt taatgttggt aaagacagtt ttgaaggtta
    22321 tacattgact atcggtaaaa aagttatcgg agaaattgcc gaattagacg gacaatttgc
    22381 cattataaag aatgggaatg tcgatagttt ttataaaaaa ttggaaaaag ctgtggaaat
    22441 tttgattgaa aattataatt tagcaaaata agtcttgttt ttttgaaatt ttcatgatat
    22501 aatagtccat gttgattgta ggagagatag cgaagaggct aaacgcggcg gactgtaaat
    22561 ccgctccttc gggttcgggg gttcgaatcc ctctctctcc atttcattaa tggggtatag
    22621 ccaagcggta aggcaaggga ctttgactcc ctcatgcgtt ggttcgaatc cagctacccc
    22681 agttcttagg taataatcaa gatagaaagc aaaatatctt agggtatttt atttttataa
    22741 ttgaaagacg tgaatgatat gaacatgtcc ttgcgggtgc ttaggaaaaa aattataagt
    22801 atgtcaagtt taagaaaaac ttgattgttg gaggattttt tagatgaacg aatttgaaga
    22861 tttgctaaat agcgttagtc aagttgagac tggtgatgtt gttagtgctg aagtattgac
    22921 agttgatgcg actcaagcta acgttgcaat ctctggaact ggtgttgaag gtgtcttgac
    22981 tcttcgcgaa ttgacaaacg atcgtgatgc agatatcaat gactttgtta aagtaggaga
    23041 agtattggat gttcttgtac ttcgtcaagt agttggtaaa gatactgata cagttacata
    23101 ccttgtatct aaaaaacgcc ttgaagctcg caaagcatgg gacaaacttg ttggtcgcga
    23161 agaagaagtt gttactgtta aaggaacgcg tgccgttaaa ggtggacttt cagtagaatt
    23221 tgaaggtgtt cgtggattta tcccagcttc aatgttggat actcgtttcg tacgtaacgc
    23281 tgagcgtttt gtaggtcaag aatttgatac taaaatcaaa gaagttaacg ctaaagaaaa
    23341 ccgcttcatc ctttcacgtc gtgaagttgt tgaagcagct actgcagcag ctcgcgctga
    23401 agtattcggt aaattggctg ttggtgatgt tgtaactggt aaagttgctc gtatcacaag
    23461 cttcggcgct ttcgtcgacc ttggtggtgt tgacggattg gttcacttga ctgaattgtc
    23521 acatgaacgt aatgtatcac caaaatcagt tgtaactgtt ggtgaagaaa ttgaagtgaa
    23581 aatccttgat cttaacgaag aagaaggacg tgtatcactt tcacttaaag caacagtacc
    23641 aggaccatgg gatggcgttg agcaaaaatt ggctaaaggt gatgtagtag aaggaacagt
    23701 taaacgtttg actgacttcg gtgcatttgt tgaagtattg ccaggtatcg atggacttgt
    23761 tcacgtatca caaatttcac acaaacggat tgaaaatcca aaagaagctc ttaaagttgg
    23821 tcaagaagtt caagttaaag ttcttgaagt taacgcagat gcagaacgcg tgtcactttc
    23881 tattaaagct cttgaagaac gtccagccca agaagaagga caaaaagaag aaaaacgtgc
    23941 tgctcgtcca cgtcgtccaa gacgtcaaga aaagcgtgat ttcgaacttc cagaaacaca
    24001 aacaggattt tcaatggctg atttgtttgg tgatatcgaa ctttaatcaa attgaaaatt
    24061 cacaaaatcc tttgtttact aaacaaggga tttttctggc tctttgtcaa ctgtagtggg
    24121 ttgaagaaaa gctaagctcg agaaaggaca aattttgtcc tttctttttt gatattcaga
    24181 gcgataaaaa tccgtttttt gaagttttca aagttccgaa aaccaaaggc attgcgcttg
    24241 ataagtttga tgagattatt ggtcgcttcc agtttggcgt tagaatagtg tagttgaagg
    24301 gtgttgacaa gcttttcttt atctttgagg aaggttttaa agacagtctg aaaaatagga
    24361 tgaacctgct taagattgtc ctcaataagt ccgaaaaatt tctccggttc cttattctga
    24421 aagtgaaaca gcaagagttg atagagctga tagtggtgtt tcaagtcttc cgaataactc
    24481 aaaagcttgt ttaaaatctc tttattggtt aagtgcatac gaaaagtagg acgataaaat
    24541 cgcttatcac tcagtttacg actatcctgt tgtatgagct tccagtagcg cttgatagcc
    24601 ttgtattcat gagactttcg atgaaactga ttcatgatct agaaatttga aagcgaagtt
    24661 gtctagctaa gtcatagtaa gggctaaaca tatccatagt aatgattttg acgcgacatc
    24721 ggacggctct atcatattta agaaagtgat ctcggatgat agcttgtgtt ctaccttcaa
    24781 gaacagtgat gatatcgagc ttgtcaaaat cttgcgcaat aaagctcatc ttcatctccc
    24841 gattgaaaca gtcactcccc ggactgtttc aacgtcccag gacataatct caggaagacg
    24901 cgaaaaatca tgctcaaagt gagaatcatt gagctttcga atgacagttg aagttgaaat
    24961 ggccagctga tgagcaatat cggtcataga aatcttctca atcaactttt gcgcaatttt
    25021 ttggttgata atacgaggaa tttggtgatt tttcttgacg atagaagttt cagcgaccat
    25081 catttttgaa cagtgatagc acttgaaacg acgctttcta agtagaattc tagtaggcat
    25141 accagttgtc tcaaggtaag gaatcttaga cggtttttga aagtcatatt tcttcaattg
    25201 gtttccgcac tcagggcaag atggggcgtc gtagtccagt ttggcgatga tttccttgtg
    25261 tgtatcttta ttgatgatgt ctaaaatctg gatattaggg tctttaatgt ctagtaattt
    25321 tgtgataaaa tgtaattgtt ccatatgaat ctttctaatg agttgtttgg tcgcttttca
    25381 ttataggtca tatgggactt tttttctaca ataaaatagg ctccataata tctataaggg
    25441 atttacccac tacaaatatt atagagccaa aaatcctttg tttactaaac aagggatttt
    25501 tcttttgtct ctgctccttt tttgatataa tagttctatg ttaaaatcag aaaaacaatc
    25561 acgttatcaa atgttaaatg aagaattgtc cttcctattg gaaggcgaaa ccaatgtttt
    25621 ggctaatctt tccaacgcca gtgctctcat aaaatcacgt tttcctaata ccgtatttgc
    25681 aggcttttat ttgttcgatg gaaaggaatt ggttttaggc cccttccaag gaggtgtttc
    25741 ctgcatccgt attgcactag gcaagggtgt ttgtggtgag gcagctcact ttcaggaaac
    25801 tgttattgtt ggagatgtga cgacctatct caactatatt tcttgtgata gtctagctaa
    25861 aagtgaaatt gtggtgccga tgatgaagaa tggtcagtta cttggagttc tggatctgga
    25921 ttcttcagag attgaggatt acgatgctat ggatcgagat tatttggaac aatttgtcgc
    25981 tattttgctt gaaaagacag catgggactt tacgatgttt gaggaaaaat cttaatgtat
    26041 caagcacttt atcgaaaata tagaagtcaa aacttctccc agttagttgg tcaagaagtt
    26101 gtggctaaga ctcttaaaca agcggtggag caagagaaaa taagtcacgc ttatcttttt
    26161 tctggtcctc gtggaacggg aaaaaccagt gttgctaaaa tctttgccaa ggctatgaac
    26221 tgtcccaatc aagtgggtgg cgaaccttgc aataactgct atatttgtca agcagtgacg
    26281 gacggtagtt tagaagatgt cattgaaatg gatgcagctt ctaataatgg ggtagatgaa
    26341 attcgcgaaa ttcgtgataa atctacctat gcgcctagcc ttgctcgtta taaggtttat
    26401 atcatagatg aggttcacat gctgtctaca ggggctttta atgccctcct aaagacgctg
    26461 gaagaaccaa cacagaatgt agtctttatt ttggccacta ctgaattgca caagattcct
    26521 gctactattc tatcccgtgt gcaacgtttt gagtttaaat caattaagac acaggatatt
    26581 aaggaacata ttcactatat cttagaaaaa gaaaatatca gttctgaacc agaggctgtg
    26641 gaaatcattg ccagacgggc ggaaggtgga atgcgggacg ccttgtctat tttggatcaa
    26701 gccctgagtt tgacacaggg aaatgagctg acgactgcta tctctgaaga aattactggc
    26761 accattagcc tatcagcctt ggatgattat gtggcggcct tgtctcaaca ggatgttccc
    26821 aaagctttgt cttgcttgaa tcttcttttt gacaatggta agagcatgac tcgttttgtg
    26881 accgatcttt tgcactattt aagagacttg ttaattgttc aaacaggggg agaaaatact
    26941 catcatagtt cagtctttgt agaaaatttg gcacttcctc aaaaaaatct gtttgaaatg
    27001 attcgcttag caacagtgag tttagcagat attaagtcta gtttgcagcc caagatttat
    27061 gctgaaatga tgaccgtccg tttggcggaa atcaagcccg aaccagctct atcaggagcg
    27121 gttgaaaatg aaattgctac gctgagacag gaagttgccc gtctcaaaca agagctttct
    27181 aatgtaggtg cggttcctaa acaagttgca ccagctccta gtcgaccagc tacgggcaaa
    27241 acagtctatc gtgtcgatcg caataaagtg caatctatct tacaagaggc cgtcgaaaat
    27301 cctgatttag cacgtcaaaa tctaattcgt ttgcagaatg cctggggaga ggtaattgaa
    27361 agtctaggtg ggccggacaa ggctctgcta gttggttctc aaccggttgc tgccaatgaa
    27421 caccatgcta ttcttgcttt tgagtctaac ttcaatgctg gtcaaactat gaaacgagac
    27481 aatctcaata ccatgtttgg taatatcctc agtcaggcgg caggtttttc acctgagatt
    27541 ttagctattt ccatggagga atggaaagaa gttcgcgcag ccttttcagc caaagccaaa
    27601 tcttctcaaa ctgaaaaaga agtagaagaa agcctgattc cagaaggatt tgaatttttg
    27661 gctgataaag tgaaggtaga ggaagactaa agaaagattt catgatacaa taagtttatg
    27721 aataaacaac aatttattat tatggcgctg tttacagctg ctgagaccta ttttttcaat
    27781 gaagcctgga tgactggccg ctatattatg gcagcctttt gggcaatttt actctttaga
    27841 aatttccgag tcagttatgt gatgggcaaa atcgttgatg tcatcgatca gcattttaat
    27901 aggaaagact agccctcagc ttccagacaa aatcaaagcc ttttaggctt ttttttgtta
    27961 tactagaaaa gtatatttat agaatttttg ctctatttct ggggaaatca gacgtttttc
    28021 tagtaagtac tgtaaaagtt ttgaaaaaga aaggaactat catgtcagta ttagagatca
    28081 aagatcttca cgttgagatt gaaggaaaag aaattttaaa aggggttaac ctgaccctga
    28141 aaacaggaga aattgccgct atcatgggac caaatggtac aggtaaatcg actctttctg
    28201 ccgctatcat gggaaatcca aactatgaag taactaaagg tgaagttttg tttgatggcg
    28261 taaacatcct tgagttggaa gtggatgagc gtgcgcgtat gggacttttc cttgctatgc
    28321 aatacccatc agaaatccct ggaattacca atgctgagtt tcttcgtgcc gctatgaatg
    28381 cgggtaaaga agatgatgag aagatttcag ttcgtgagtt tattactaag ctagatgaaa
    28441 aaatggaatt gctcaacatg aaagaagaaa tggcagagcg ttacctcaac gaaggcttct
    28501 ctggtggtga gaaaaaacgc aatgaaattc ttcaactttt gatgttggag ccaacatttg
    28561 ctcttttgga cgagattgac tcaggtcttg atattgacgc tcttaaagtt gtgtctaaag
    28621 gtgtcaatgc catgcgtggt gaaggttttg gtgctatgat catcactcac taccaacgtc
    28681 ttttgaacta tatcacacct gatgtggtac acgtgatgat ggaaggtcgt gttgtccttt
    28741 ctggtggtcc agaattggct gcgcgtttgg aacgtgaagg atacgcaaaa ttagctgaag
    28801 aacttggcta cgactacaag gaagaattgt aattccctcg tatcttttag gagaagtaaa
    28861 tgactagaga aaatattaaa cttttttcag aaatgcacgc tgaaccaagc tggttggctg
    28921 atctccgtca aaaagctttt gacaagattg agactttgga attaccagtt attgagtgtg
    28981 tcaaattcca ccgttggaat ctgggtgatg gaacgattac agaaaatgag ccatcagcaa
    29041 atgttccaga tttcacagct ttagatcatc acttgaagtt ggtgcaagta ggaactcaaa
    29101 ctgttttcga acaaactcca gttgagttag ctgaacaggg tgttgtcttc acagactttc
    29161 actcagcttt agaagaaatt ccagagctga tcgaagaatt cttcatgtca tctgttaagt
    29221 atgatgatga caagttggcg gcttaccaca cagcttactt taacagtggt gctgtactct
    29281 atattccaga taacgtagaa atcacagagc caattgaagg aattttctac caagatagcg
    29341 atagcaatgt gccgtttaac aagcatatta tgattatcgt tggtaaaaat tctaagatta
    29401 gttatctgga gcgtttagag tcacgcggtg aaggaagtga caaagcaact gccaatatca
    29461 cagtggaagt gattgcacgt tctggtgcgc aagtcaagtt tgctgctatc gaccgtctag
    29521 gtgaaaacgt cactgcctac attagccgtc gtggtaaatt aggcaacgat gcaagtattg
    29581 actgggctat cggtgtcatg aacgaaggaa atgtcgttgc tgattttgat agtgacttga
    29641 ttggtaatgg tagccatgct gacctcaagg ttgtagctct ttcaagtggt cgtcaggtac
    29701 aagggattga tactcgtgta actaactatg gctgcaactc aatcggaaac attctacaac
    29761 atggggttat ccttgaaaaa gcaactttga ctttcaatgg tatcggccac atcatcaagg
    29821 gtgctaaggg agcagatgcg caacaagaga gccgtgttct catgctttca gaccaagcgc
    29881 gttcagatgc taacccaatt cttttgattg atgaaaatga cgtaactgca ggccatgcag
    29941 cctctattgg tcaggtagat ccagaagata tgtactacct catgagtcgt ggcttggata
    30001 aggcaactgc agagcgtttg gttgttcgtg gtttccttgg atctgttatc gtggagattc
    30061 cagtcaagga agttcgtgat gaaatgattg caactatcga agagaaattg tcaaaacgct
    30121 aaggggcagc ctatgttaga tgtagaagcg attcgcaagg attttccaat tttagatcag
    30181 attgtcaatg atgaacctct ggtctatctg gacaatgctg cgacgacaca aaaaccacta
    30241 gtagttctga aagctattaa cagctactat gagcaggaca atgccaatgt tcaccgtggt
    30301 gtccatacct tagcggaacg agcgacagct tcttatgaag ctgctcgtga aaccattcgt
    30361 aagtttatta atgcaggctc tacaaaggaa gttctcttta ccagaggaac gacaaccagc
    30421 cttaactggg tggcacgctt tgctgaggaa attctcactg agggagacca ggtcttgatt
    30481 tcagtaatgg aacaccattc taatatcatt ccatggcagg aagcttgtcg aaagactgga
    30541 gcagagcttg tctatgtcta tcttaaagac ggtgccttgg atatggagga tttgcgagct
    30601 aaattgactg ataaggttaa atttgtttcc ctagctcatg cctccaatgt tcttggtgtg
    30661 gtcaatccga tcaaggaaat cactcaatta gcccaccaag ttggggcaat tatggtagtg
    30721 gatggtgctc aatctacacc tcatatgaag attgatgtcc aggacttgga tctggacttt
    30781 ttcgcctttt cgggtcacaa gatggctggt ccgactggta tcggtgtcct ttacggcaaa
    30841 gaaaagtatc ttgagcaaat gtctccagta gaatttggcg gcgagatgat tgattttgtc
    30901 tacgagcaat ttgctagttg gaaggaattg ccttggaaat ttgaggctgg aacgccaaat
    30961 atggcaggag ctattggact tgcgactgca gttgattatc tggaaaagat tggtatggat
    31021 gccgttgaag ctcatgaaca ggaattgatt gcgtacgtct atccaaaact gcaggcaatt
    31081 gagggattga ccatttacgg ttctcaggat ttggctcaac gttcgggtgt tattgccttt
    31141 aacctaggtg atctccatcc tcacgatctt gcgacagctc tggattatga aggagtggct
    31201 gttcgtgctg gtcaccattg tgcgcaaccc ttgcttcagt atttggaagt cccagcaaca
    31261 gctcgtgcaa gtttttatat ctataatacc aaggcagatt gcgacaaact agtcgatgcc
    31321 ctacaaaaga caaaggagtt tttcaatggc actttctaaa ctagatagcc tttatatggc
    31381 agtggtagca gaccattcga aaaatccaca tcaccaaggg aagttagaag acgctgagca
    31441 aatcagtctc aacaatccga cttgtggaga tgtcatcaat ctctctgtca agtttgatgc
    31501 agaggaccgt ttggaagata ttgcttttct aaattcagga tgcacgattt caactgcttc
    31561 tgctagtatg atgacagatg ccgttttagg aaaaaccaaa caagaaattt tagaactggc
    31621 gactattttt tctgaaatgg ttcaagggca aaaagatgag cgtcaagacc aacttggaga
    31681 cgcggcattc ttgtcaggtg ttgccaaatt ccctcaaaga atcaagtgtg caaccctagc
    31741 ttggaatgcc cttaagaaaa caattgaaaa tcaagaaaaa cagtaagaca agtttctttt
    31801 gtcttatgaa ttattagaaa tgaagaaaga aaggatatta tggctgaaga aagagtagaa
    31861 ccaaaaccaa ttgaccttgg tgaatataaa tttggtttcc atgacgatgt agagcctgtc
    31921 ttatcgacag gaaaaggact caacgaaggt gttattcgtg aattatctgc tgctaagggt
    31981 gagcctgagt ggatgttgga gttccgtttg aagtcttatg aaaccttcaa aaaaatgccc
    32041 atgcaaactt ggggagcaga cttgtcagag attgactttg atgacttaat ctactaccaa
    32101 aaaccatctg acaaaccagc ccgttcttgg gatgatgtac ctgaaaagat taaagaaacc
    32161 tttgaacgta tcgggattcc agaagctgaa cgtgcttatt tagcaggggc ttctgcccag
    32221 tacgagtcag aagtggttta ccacaacatg aaggaagagt tccaaaaatt aggtattatc
    32281 tttacagata cagattccgc actcaaggaa tacccagact tatttaatca atactttgcg
    32341 aagttggtac cgccgacaga taacaagttg gcagccctca actcagcagt atggtcgggt
    32401 ggaactttta tctacgtgcc aaaaggtgtc aaggtagata ttccacttca aacttatttc
    32461 cgtatcaata acgaaaatat aggtcagttc gaacgtacct tgattatcgt tgatgaggga
    32521 gcaagcgtcc actacgtaga aggatgtaca gcaccaacat attcaagcaa tagcttacac
    32581 gctgccattg tagaaatttt tgctttggac ggagcttata tgcgttatac aactatccaa
    32641 aactggtctg ataacgtcta taacttggta acaaagcgtg ctaaggctca aaaggatgcc
    32701 actgttgagt ggattgatgg aaacttgggt gccaaaacga ctatgaaata tccatctgtt
    32761 taccttgatg gagaaggagc gcgtggtacc atgctctcta tcgcctttgc taatgcaggg
    32821 caacaccaag acacgggtgc taagatgatt cacaatgctc cacataccag ctcgtctatt
    32881 gtgtctaaat ccatcgctaa aggtggagga aaggttgact accgtggaca agtcaccttt
    32941 aacaagaact ctaagaaatc tgtttcccac attgaatgtg ataccattat catggatgac
    33001 ttgtcagcat cagatactat tccatttaat gaaattcaca actcgcaagt ggctttggaa
    33061 cacgaagcca aagtatctaa gatttcagaa gagcaattgt attatctcat gagccgtgga
    33121 ttgtcagaat ctgaggcaac tgaaatgatt gtcatgggat ttgtagaacc ctttacaaaa
    33181 gaacttccaa tggaatacgc agttgagctg aaccgcttga ttagctatga aatggaggga
    33241 tcagttggat aaaatttgat tttatactct tcgaaaatct cttcaaacca cgtcagcatc
    33301 gccttaccgt atgtatggtt actgacttcg tcagtttcat ctacaacctc aaaacagtgt
    33361 tttgagcaac ctgcggctag cttcctagtt tgttctttga ttttgaagat gaacttgtat
    33421 caaaaaatcg cggtttaaaa tcgcgatttt ttataatttc tcgttaacaa aacggacaaa
    33481 ctgattccac caaactttta agaagaaggc tttttcaatt ttcttgtctg ctaccatttc
    33541 gaaactaggg cgctctgtgg tgatgtaacc ttgaccaatc aagtccttgt cttcataagt
    33601 caaatggcca accactgttc cagcttcaag tggtgctggg attgctttgg aatcaggtgt
    33661 gaattgaaca gattgggaag attgattccc aacacgttcg attagataga tatcctctgg
    33721 agccactgca gttactgtat cttcttttcc atcttgtaca ggggctttgc tatcttgata
    33781 ggcatcgcct tgttgaacga ttttgcgaag tgtaaatgta gaagaaatat aatccattag
    33841 ggaagatgta gctgtaaatc gagcgtaagg attattgtct tgatgatctg catttaaaac
    33901 aactgtgatg actctcatgc ctttttcgac agtagtacca acaaaagact ctccagcctt
    33961 atctgttgtt cctgttttta gcccatcaaa accaccacgg taagcaggca taccttctaa
    34021 catgtagttg gttgaagtga ttgtcatccc agcaaaagta gaagaaggtt ttttggtgat
    34081 ttctaagact tgtgggtatt ttttgatgag gttgcgagca acgatagcga catcataagc
    34141 actaagctta ttttcctcat cttttttaga acctgggtaa atgttatccc ctagagtttc
    34201 attgttaaga cctgtcgtat tgacaacagt ggcatcctga attccccatt ccaagagttt
    34261 tgcccgcatc atatcgacga aatctttttc tgagccagca attttctcag ctagggcaat
    34321 agcggcgctg ttggcactag ataccagagt tgcttcaagc aactcttcga cagtataatt
    34381 acgggcctcc ataggaatat tactggcttc agaatttgtc gtcaattgat aaggataatc
    34441 agaaatatct acaggagtgg agagggtaat acttccgttt tccaaagctt catagaccag
    34501 ataaacagta atcaattttg ttatggaagc aatttcgaca ggttgcgttg catccttctc
    34561 atagagaatt ttaccagtat ttgcctcaac agcaatcgca tgtttagcgg caatggtaaa
    34621 atcttgagca acagcagtag aagcaccccc taaaagagag acagttaaca aagttaaaaa
    34681 tatttttttc atagtagtct tattctatca taaagaaaaa aaatattctt gctttaataa
    34741 ttcatctgtt aagctttttg aaaatatggt aaaataaagt gagggaggta actcatgttt
    34801 cgtagaaata aattattttt ttggaccaca gaaattttac tcttaaccat catcttttac
    34861 ctatggagac agatggggtc tttgattaac ccttttgtta gcgtgcttaa tacaattatg
    34921 attccatttt tattaggggg ctttctttat tatttgacaa accctattgt tactttctta
    34981 aataaagtct gtaaactcaa tcgtttgctt ggtattttaa ttaccttgtg tactttggtc
    35041 tggggaatgg tcataggtgt tgtctatctc ttacctattt tgattaatca gttatctagt
    35101 ttgattatat ctagtcaaac tatttatagt cgagtacaag acttaatcat agacttatct
    35161 aattatcctg cgctccagaa tttggatgta gaagctacaa ttcagcagtt aaacttatcc
    35221 tatgtggata ttcttcaaaa tatcctaaat agcgtatcaa atagtgtggg gagcgtcttg
    35281 tcagctctta tcagtactgt tttgattttg attatgactc cagttttttt ggtttatttc
    35341 ttattagatg gacataaatt cttgcccatg cttgaaagaa cgattctaaa gagggatcgc
    35401 ttgcatattg caggcttatt aaagaattta aatgcgacga ttgctcgcta tattagtgga
    35461 gtttcgattg acgcaatcat tataggttgt ttagcttata ttggctatag tattattggt
    35521 ttaaaatatg ctttagtttt tgccattttt tctggtgtag ccaatttaat tccttatgtg
    35581 gggccaagta ttggtttgat tcctatgatc atcgcaaata tattcactga tccccataga
    35641 ctgctgattg cagtgattta tatgcttgtt gttcagcagg tagatggcaa tatcttatat
    35701 cctcgaattg taggaagtgt tatgaaggtt catccaatca cgattttagt tttacttttg
    35761 ttgtcaagca atatctatgg tgtagttgga atgattgtcg cagtgccaac ctattctatc
    35821 ttgaaagaaa tttctaagtt cttatcccgt ttgtatgaaa atcataaaat aatgaaagaa
    35881 cgagaaagag aattagctaa gtaaaagtca ggagaaccct gatttttctt tactggaagt
    35941 ggcctttaga ttagaagact gaaaataagt taaagtctta aactaatttt cacagctaag
    36001 aatagtagaa gttaatctga taaaaatcga aaaaaccagt ggaattccgt gtcagggtaa
    36061 gttccactgg ttttcatagt ctattaaagt tcgaatgaaa cctatttata gtatattgaa
    36121 actagaatag tacacctcta attctaaaac attgttagaa atcgatttga ctgtcctgat
    36181 ctattcgtcc tattcttatt tcattttact atattttggt gcaataagtg aaaagtagtc
    36241 cgaataatat aaggattgat tttatagttt ttaaactcaa atgaattaaa ataaagagag
    36301 tacgaaaatt ctcatctgaa agtattttag aataattctc ttcgtgaatt tcttcaaaac
    36361 agatagcttc atcttaggta tgtgttaatt ggatttcgta agctctattt acaatcgtaa
    36421 agttatgcac tgaaaaagtt gtagttagtc ttccagtttg ctaaatttct cgaatagtat
    36481 cttttttagg tatgtgattt ctttttgcat ttttgagtta gataaggtat aatggtttta
    36541 ttgtcttttg gggtcgttac ggattcgaca ggcattatga ggcatatttt gcgactcgtg
    36601 tggcgacgta aacgctcagt taaatataac tgcaaaaaat aacacttctt acgctctagc
    36661 tgcctaaaaa ccagcaggcg tgacccgatt tggattgctc gtgttcaatg acaggtctta
    36721 ttattagcga gatacgatta agccttgtct agcggcttga taagagattg atagactcgc
    36781 agtttctaga cttgagttat gtgtcgaggg gctgttaaaa taatacataa cctatggttg
    36841 tagacaaata tgttggcagg tgtttggacg tgggttcgac tcccaccggc tccattattc
    36901 ctttgcattc ttttgcattc cttggtaaaa cgttgttaaa tcaacgtttt ttatttttat
    36961 ctttggtatt cctttgcatt cttttgctaa aaagggagtc acaaacagac cctattttaa
    37021 aaaaaggata gaaaaaagga tacaacattt gtcgcatcct aaaaataatc ttttttcgac
    37081 ggaagacatg ggattcgaac ccacgcacgc tattacacgc ctaccgcgtt tccaacacgg
    37141 cctcttaagc ctcttgagta atcttccaat acttactcaa atagtctacc ataaaggctc
    37201 ttatcttgca ataaaaattc tagaaataag aaaaaa
//
LOCUS       DAVHEX010000013        34931 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVHEX010000013 DAVHEX010000000
VERSION     DAVHEX010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34931
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.013"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            16..255
                     /locus_tag="VVL45_000648"
     CDS             16..255
                     /locus_tag="VVL45_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU4186855.1"
                     /translation="MPYFVSQEVYWKVRNIEAEAIRRNCERGAIFSGKIKYHEDSQFK
                     GDHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
     gene            500..1834
                     /gene="trmFO"
                     /locus_tag="VVL45_000649"
     CDS             500..1834
                     /gene="trmFO"
                     /locus_tag="VVL45_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEU4186856.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVVNHPLIEVVRDEITELPTDVITVVATGPLTSDALAEKIHALNNGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            1918..2655
                     /locus_tag="VVL45_000650"
     CDS             1918..2655
                     /locus_tag="VVL45_000650"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEU4186857.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            2664..3221
                     /gene="frr"
                     /locus_tag="VVL45_000651"
     CDS             2664..3221
                     /gene="frr"
                     /locus_tag="VVL45_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEU4186858.1"
                     /translation="MANVIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            3281..4135
                     /gene="cvfB"
                     /locus_tag="VVL45_000652"
     CDS             3281..4135
                     /gene="cvfB"
                     /locus_tag="VVL45_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEU4186859.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            4144..4359
                     /locus_tag="VVL45_000653"
     CDS             4144..4359
                     /locus_tag="VVL45_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEU4186860.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            4445..5413
                     /locus_tag="VVL45_000654"
     CDS             4445..5413
                     /locus_tag="VVL45_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEU4186861.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            complement(5391..5570)
                     /locus_tag="VVL45_000655"
     CDS             complement(5391..5570)
                     /locus_tag="VVL45_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4186862.1"
                     /translation="MTQEDALIVISHIKVLSIVPNRCLRPLDKTFSLYNWIFLSQKYI
                     LLQANFLKISRVQLQ"
     gene            5606..6106
                     /locus_tag="VVL45_000656"
     CDS             5606..6106
                     /locus_tag="VVL45_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4186863.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVMDFVYAHPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIV
                     EQSLED"
     gene            6109..6369
                     /locus_tag="VVL45_000657"
                     /pseudo
     CDS             6109..6369
                     /locus_tag="VVL45_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(6736..7823)
                     /gene="ald"
                     /locus_tag="VVL45_000658"
                     /pseudo
     CDS             complement(6736..7823)
                     /gene="ald"
                     /locus_tag="VVL45_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253293.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
     gene            8000..8569
                     /locus_tag="VVL45_000659"
     CDS             8000..8569
                     /locus_tag="VVL45_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4186864.1"
                     /translation="MESIFVKFAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKDNGKFIGTIDLHKIDPVLKKAAIGYI
                     INKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKANPASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            8637..9287
                     /locus_tag="VVL45_000660"
     CDS             8637..9287
                     /locus_tag="VVL45_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEU4186865.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     AEVAAVSKDSSTGKEVNKEEKEEPLEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSTSKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGAKTIEKLKDYVTVD"
     gene            9271..11511
                     /locus_tag="VVL45_000661"
     CDS             9271..11511
                     /locus_tag="VVL45_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEU4186866.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSVSYFALLGFVFLLVCL
                     FFQFPWKSASKVLVICGIFGFWFVFQNWQQSQASQNLADSVEGVRILPDTIKVNGDSL
                     SFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
                     LKTQGIYQTLNIKTIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVASESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     TFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVARRPLVFGQPNAWLLILLLI
                     SLALVYDLRKNIKGLTVLSLLITGLFFLTKYPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIEKWQEKMTTSNAQRSLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     LEVTKAFHVGEILVSKGSLKQKQFVVELQATQTKVRSMTVGENLPIFGSQLEVLSLRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPTFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            11910..12497
                     /locus_tag="VVL45_000662"
     CDS             11910..12497
                     /locus_tag="VVL45_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4186867.1"
                     /translation="MLQLTHVTLKTRQVILQDADFTFKKGRFYGLLAINGSGKTTLFR
                     AISNLIPISSGNIATPPSLFYYESIEWLDGNLSGMDYLRLIKNIWKSDLNLRDEIAYW
                     EMADYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDICDRVVTIHQGQIEEV"
     gene            12501..13682
                     /locus_tag="VVL45_000663"
     CDS             12501..13682
                     /locus_tag="VVL45_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4186868.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIILLLFASVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEAKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWQDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEAIIPTLFVIGIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYSIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLSKQIDNVDLNWSMGMVLLPCLIILLLVGILFIESWGSSRKKEVFK
                     V"
     misc_feature    13842..13973
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            13989..14519
                     /gene="infC"
                     /locus_tag="VVL45_000664"
     CDS             13989..14519
                     /gene="infC"
                     /locus_tag="VVL45_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEU4186869.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            14552..14752
                     /gene="rpmI"
                     /locus_tag="VVL45_000665"
     CDS             14552..14752
                     /gene="rpmI"
                     /locus_tag="VVL45_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEU4186870.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            14804..15163
                     /gene="rplT"
                     /locus_tag="VVL45_000666"
     CDS             14804..15163
                     /gene="rplT"
                     /locus_tag="VVL45_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEU4186871.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            15221..15601
                     /locus_tag="VVL45_000667"
     CDS             15221..15601
                     /locus_tag="VVL45_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4186872.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            15851..16651
                     /locus_tag="VVL45_000668"
     CDS             15851..16651
                     /locus_tag="VVL45_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEU4186873.1"
                     /translation="MNPTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDNHP
                     RAYLSLESRMACGMGACYACVLKVPENETVSQRVCEDGPVFRTGTVVL"
     gene            16614..17600
                     /locus_tag="VVL45_000669"
     CDS             16614..17600
                     /locus_tag="VVL45_000669"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEU4186874.1"
                     /translation="MVLFSAQEQLYYKEKIMTTNRLQVSLPGLDLKNPIIPASGCFGF
                     GQEYAKYYDLDLLGSIMIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAE
                     KLPWLEREYPNLLIIANVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGL
                     LIGQDPDLAYDVVKAAVEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVG
                     MRFDLKTRKPILANGTGGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEM
                     YLAGASAIGVGTANFTNPYACPDIIENLPKVMDKYGISSLEELRQEVKEFLR"
     gene            17642..19675
                     /locus_tag="VVL45_000670"
     CDS             17642..19675
                     /locus_tag="VVL45_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEU4186875.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGAIGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGVKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKIAEKEWVYDS
                     HSQAWYYFKSGGYMAANEWIWDKKSWFYLKFDGKMAEKEWVYDSHSQAWYYFKSGGYM
                     TANEWIWDKESWFYLKSDGKMAEKEWVYDSHSQAWYYFKSGGYMAKNETVDGYQLGSD
                     GKWLGGKATNKNAAYYQVVPVTANVYDSDGEKLSYISQGSVVWLDKDRKSDDKRLAIT
                     ISGLSGYMKTEDLQALDASKDFIPYYESDGHRFYHYVAQNASIPVASHLSDMEVGKKY
                     YSADGLHFDGFKLENPFLFKDLTEATNYSAEELDKVFSLLNINNSLLENKGATFKEAE
                     EHYHINALYLLAHSALESNWGRSKIAKDKNNFFGITAYDTTPYLSAKTFDDVDKGILG
                     ATKWIKENYIDRGRTFLGNKASGMNVEYASDPYWGEKIASVMMKINEKLGGKD"
     gene            complement(19736..21391)
                     /gene="pavA"
                     /locus_tag="VVL45_000671"
     CDS             complement(19736..21391)
                     /gene="pavA"
                     /locus_tag="VVL45_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HEU4186876.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEM
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTESLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            21496..21993
                     /gene="ybeY"
                     /locus_tag="VVL45_000672"
     CDS             21496..21993
                     /gene="ybeY"
                     /locus_tag="VVL45_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEU4186877.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            21974..22369
                     /locus_tag="VVL45_000673"
     CDS             21974..22369
                     /locus_tag="VVL45_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEU4186878.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFGFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            22386..23285
                     /gene="era"
                     /locus_tag="VVL45_000674"
     CDS             22386..23285
                     /gene="era"
                     /locus_tag="VVL45_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEU4186879.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            23334..24158
                     /gene="mutM"
                     /locus_tag="VVL45_000675"
     CDS             23334..24158
                     /gene="mutM"
                     /locus_tag="VVL45_000675"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEU4186880.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMERRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARSSQTLTAEEATAIHDQTIAVLSQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            24158..24763
                     /locus_tag="VVL45_000676"
     CDS             24158..24763
                     /locus_tag="VVL45_000676"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEU4186881.1"
                     /translation="MGKIIGITGGIASGKSTVTNFLRQQGFQVVDADAVVHQLQKPGG
                     RLFEALVQHFGQEIILENGELNRPLLASLIFSNPDEREWSKQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            24750..25949
                     /locus_tag="VVL45_000677"
     CDS             24750..25949
                     /locus_tag="VVL45_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter PmrA"
                     /protein_id="HEU4186882.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSQQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            25949..26098
                     /gene="rpmG"
                     /locus_tag="VVL45_000678"
     CDS             25949..26098
                     /gene="rpmG"
                     /locus_tag="VVL45_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU4186883.1"
                     /translation="MRVKINLKCSSCDSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            26138..26371
                     /gene="secG"
                     /locus_tag="VVL45_000679"
     CDS             26138..26371
                     /gene="secG"
                     /locus_tag="VVL45_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEU4186884.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            26473..28827
                     /gene="rnr"
                     /locus_tag="VVL45_000680"
     CDS             26473..28827
                     /gene="rnr"
                     /locus_tag="VVL45_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEU4186885.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEAKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVSSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSRSGRGRGSNRRSDKKEDKRKL
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            28790..29257
                     /gene="smpB"
                     /locus_tag="VVL45_000681"
     CDS             28790..29257
                     /gene="smpB"
                     /locus_tag="VVL45_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEU4186886.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            29273..30133
                     /gene="tehB"
                     /locus_tag="VVL45_000682"
     CDS             29273..30133
                     /gene="tehB"
                     /locus_tag="VVL45_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEU4186887.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPIAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            30206..31159
                     /gene="coiA"
                     /locus_tag="VVL45_000683"
     CDS             30206..31159
                     /gene="coiA"
                     /locus_tag="VVL45_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEU4186888.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQIHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKRQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            31178..32980
                     /gene="pepF"
                     /locus_tag="VVL45_000684"
     CDS             31178..32980
                     /gene="pepF"
                     /locus_tag="VVL45_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEU4186889.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQIEKLYAYAYMKNDQDTREAKYQEYYAKAMTLYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            32982..33695
                     /locus_tag="VVL45_000685"
     CDS             32982..33695
                     /locus_tag="VVL45_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEU4186890.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            33762..34703
                     /gene="prsA"
                     /locus_tag="VVL45_000686"
     CDS             33762..34703
                     /gene="prsA"
                     /locus_tag="VVL45_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEU4186891.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKSNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(34821..>34931)
                     /locus_tag="VVL45_000687"
     CDS             complement(34821..>34931)
                     /locus_tag="VVL45_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEU4186892.1"
                     /translation="QLERAIIDYIDYYNNKRIKIKLKGLSSVQYRTKSFG"
BASE COUNT        10512 a         6472 c         7889 g        10058 t
ORIGIN      
        1 attcatagaa ctactatacc atattttgtt tcgcaggaag tctattggaa agtaagaaat
       61 attgaagctg aggctattag aagaaattgt gagcgtggtg ctattttttc aggtaaaata
      121 aaatatcacg aagattcaca gtttaaagga gatcactatg ttgaatgtta tgctgtttta
      181 gataatacgg ttatagcaag agatagaata acagtcccta tcgatccgtt atgtggaaaa
      241 gattttatag agtagcatat aattgattct taactggaat actcactatc tctttacatc
      301 aagaaaatga ctaaacaggg aagtttgcct tcttcccttt ttttgttata ctagtagaag
      361 aaaaaataga aagatttgtg ggagtgaaaa agtcctgtgg actttttcag cctgagccaa
      421 gaaactcgaa agcttgtaag tctgattggc tttttcaatg tgaatcttaa cttcacactc
      481 ccaaagaggt attagtgtcg tgtctcaatc ttatatcaat gttatcggtg ctggtttggc
      541 aggttctgaa gcagcttacc aaatcgcaga gcgtggtatt ccagttaaac tatatgaaat
      601 gcgtggtgtc aagtctacac cccagcataa aacagacaat tttgctgagt tggtttgttc
      661 caattctttg cgtggggatg ctttgacaaa tgcagttggt cttctcaagg aagaaatgcg
      721 tcgcttgggt tctgttatct tggaatctgc tgaggctaca cgtgttcctg ccggtggagc
      781 ccttgcggtg gatcgtgatg gtttctctca aatggtgacc gaaaaagtgg tcaaccaccc
      841 cttgattgaa gtggtgcgtg atgaaattac agaattgcca acagatgtta ttacagttgt
      901 ggcgactggt cccttgacta gcgatgccct ggctgaaaag attcatgccc ttaataatgg
      961 cgatggcttc tatttctacg atgcagcagc gcctattatc gatgtcaaca ctatcgatat
     1021 gagtaaggtc tatctcaaat ctcgttatga taagggagaa gcagcctatc tcaatgctcc
     1081 aatgaccaag caagagttta tggatttcca tgaagccttg gtcaatgcgg aagaagcacc
     1141 gctcaattct tttgaaaaag aaaagtactt tgaaggatgt atgccaatcg aagttatggc
     1201 caaacgtggc attaaaacta tgctttatgg ccctatgaag ccagtcggtc ttgagtatcc
     1261 ggacgactac acaggacctc gtgatggaga atttaaaaca ccttatgcgg ttgtgcaact
     1321 tcgtcaggat aatgcagctg gtagcctata caatattgtt ggtttccaga cccacctcaa
     1381 atggggagaa caaaagcgtg tcttccaaat gattccgggt cttgaaaatg cggagtttgt
     1441 ccgttatggt gtcatgcatc gcaattctta catggattca ccaaatcttc ttgagcagac
     1501 ttaccgttcc aagaaacaac caaatctctt ctttgctggt cagatgacgg gggtggaagg
     1561 ctatgttgag tcggcggctt cgggcttagt tgcgggaatt aacgcagctc gtctcttcaa
     1621 ggaagaaagc gaggttattt tccccgagac gacagcgatt ggaagcttag ctcattacat
     1681 tacccatgcc gacagcaaac atttccaacc aatgaatgtc aattttggga tcatcaagga
     1741 gttggaaggc gagcgtatcc gtgataagaa ggctcgttat gaaaaaattg cagagcgtgc
     1801 ccttgccgac ttagaggaat ttttgactgt ctaatttttt tgaaagaatt gttcatgata
     1861 ctataaaaat cttagaaatt gtgataaaat aggtaggatg aaagaaggag agtgaaaatg
     1921 gcgaatccca agtataaacg tattttaatc aagttatcag gtgaagccct tgccggtgaa
     1981 cgtggcgtag ggattgatat ccaaacagtt caaacaatcg caaaagagat tcaagaagtt
     2041 catagcttag gtatcgaaat tgcccttgtt attggtggag gaaatctctg gcgtggagaa
     2101 cctgcagcag aagcaggtat ggaccgtgtt caggcagatt acactggaat gcttgggact
     2161 gttatgaatg ctcttgtgat ggcagattca ttgcaacaag ttggggttga tacgcgtgta
     2221 caaacagcta ttgctatgca acaagtggca gagccttatg tccgtggacg tgcccttcgt
     2281 caccttgaaa aaggccgtat cgttatcttt ggtgctggaa ttggttcacc atacttctcg
     2341 acagatacaa cagcggctct tcgtgcagct gaaatcgaag cagatgccat cctcatggct
     2401 aaaaatggcg tcgatggtgt gtacaatgcc gatcctaaga aggacaagac agccgttaag
     2461 tttgaagaat tgacccaccg tgatgttatc aacaaaggtc ttcgtatcat ggactcaaca
     2521 gcctcaaccc tctcaatgga caacgacatt gacttggttg tcttcaacat gaaccaatca
     2581 ggcaacatca aacgtgtcgt atttggtgaa aatatcggaa caacagtttc aaataatatc
     2641 gaagaaaagg aataagaaag aatatggcta acgtaattat tgaaaaagct aaagagagaa
     2701 tgacccagtc tcaccaatca cttgctcgtg aatttggtgg tatccgtgct ggtcgtgcca
     2761 atgcaagctt gcttgaccgt gtacatgtag aatactatgg agtcgaaact cctcttaacc
     2821 aaatcgcttc aattacgatt ccagaagcgc gtgttttgtt ggtaacacca tttgacaagt
     2881 cttcattgaa agacatcgaa cgtgccttga acgcttctga tcttggtatc acaccggcta
     2941 atgacggttc tgtgattcgc ttggttatcc cagctcttac agaagaaact cgtcgtgacc
     3001 ttgctaaaga agtgaagaag gtcggcgaaa atgctaaagt ggctgtccgc aatatccgtc
     3061 gcgatgctat ggacgaagct aagaaacaag aaaaagcaaa agaaatcact gaagacgaat
     3121 tgaagactct tgaaaaagac attcaaaaag taacagacga tgctgttaaa cacattgacg
     3181 acatgactgc taacaaagag aaagaacttt tggaagtcta aaaataaaca gaaaaactca
     3241 gttggcattg ctggctgagt tttattcgaa agaaggaaat atgaatacaa atcttgcaag
     3301 ttttatcgtt ggactgatca tcgatgaaaa cgaccgtttt tactttgtgc aaaaggatgg
     3361 tcaaacctat gctcttgcta aggaagaagg ccaacataca gtaggggata cggtcaaagg
     3421 ttttgcatac acggatatga agcaaaaact ccgcctgaca accttagaag tgactgccac
     3481 tcaggaccaa tttggttggg gacgtgtcac agaggttcgt aaggacttgg gtgtctttgt
     3541 ggatacaggc cttcctgaca aggaaatcgt tgtgtcactc gatattctcc ctgagctcaa
     3601 ggaactctgg cctaagaagg gcgaccaact ctacatccgt cttgaagtgg ataagaaaga
     3661 ccgtatctgg ggcctcttgg cttatcaaga agacttccaa cgtcttgctc gtcctgccta
     3721 caacaacatg cagaaccaaa actggccagc cattgtttac cgtctcaagc tgtcaggaac
     3781 ttttgtttac ctaccagaaa ataatatgct tggttttatt catcctagcg agcgttacgc
     3841 agagccacgt ttggggcaag tattagatgc gcgcgttatt ggtttccgtg aagtggaccg
     3901 cactctgaac ctctccctca aaccacgctc ctttgaaatg ttggaaaacg atgctcagat
     3961 gattttgact tatttggaaa gtaatggcgg tttcatgacc ttaaatgata agtcatctcc
     4021 agacgacatc aaggcaacct ttggcatttc taaaggtcag ttcaagaaag ccttaggtgg
     4081 tcttatgaag gctggtaaaa tcaagcagga ccagtttggg acagagttga tttagggagg
     4141 cttatgagaa aatcatttta cacttggctc atgaccgagc gcaatcctaa aagtaacagt
     4201 cccaaagcaa ttttggcaga cctcgctttt gaagaggcag cttttccaaa acatacagat
     4261 gattttgatg aggtcagtcg ctttttggag gagcatgcca gtttctcttt taacctagga
     4321 gattttgaca gcatttggca ggaatatcta gaacactagc atttattcat tgggtttggg
     4381 ctagtaattt ctccatccct ctgctataat aaaaagaaat aaaaggatta gagaggttct
     4441 ttatttgaag gaacattcaa tagacattca actgagtcat ccagatgacc tgtttcatct
     4501 ttttggttcc aatgaacgcc atcttcgttt gatggaagaa gagcttgatg ttgtgattca
     4561 tgctcgtacg gagattgtcc aggttttggg agaagagtct gcctgtgagg aagcccgtca
     4621 agttattcag gctttgatgg tcttggtaaa tcgtgggatg accgttggta cgccagatgt
     4681 agtcactgcg attagcatgg tcaaaaacga tgaaattgat aagtttgtcg ccctttacga
     4741 agaagaaatt atcaaggata atactgggaa accgatccgc gtcaaaacct taggtcaaaa
     4801 actttatgtg gacagtgtca aacagcatga tgtgaccttt ggaattgggc ctgcagggac
     4861 ggggaagacc tttcttgcag ttactttggc agtgactgcc cttaaacgtg ggcaagtcaa
     4921 gcggattatc ttgacacgtc cagcggtgga agcaggagag agtctaggtt ttcttccggg
     4981 tgatcttaag gagaaggtag atccttacct tagacctgtt tatgatgcct tgtatcagat
     5041 tctggggaaa gaccaaacga ctcgtctcat ggagcgtgaa attatcgaaa ttgcgcccct
     5101 tgcctatatg cgtggtcgga ccttggatga tgcctttgtc attctcgatg aagcgcaaaa
     5161 cacgaccatc atgcagatga agatgttctt gacgcgttta ggctttcatt ctaagatgat
     5221 tgtcaatggc gatatcagtc agattgacct gccacgcaat gtcaagtcag gtttgattga
     5281 tgcccaagag aaactcaaga acatccatca gattgacttt gttcattttt cagctaagga
     5341 tgtggttcgc catccagttg tcgctcagat tatccgagct tatgaatatt ctactgaagt
     5401 tgcacacgac tgattttgag gaagttcgcc tgcaaaagaa tatacttttg agagaggaaa
     5461 atccagttgt ataggctaaa ggttttatcc aaaggtctga gacaacgatt aggcacgatg
     5521 gaaagaactt ttatgtggct gatgacgatc agtgcatctt cctgtgtcat aatcacaggg
     5581 cacaagaaag taggaatttg aaaagatgat tgaccaacta tctaagtatt acagttgtag
     5641 gatactaact gaaaaggata ttccaagtat tttatcttta tatgaaagta atcctctgta
     5701 ttttcagcat tgtccaccag agccaaattt tgcaactgta aaagaggaca tgctttgtct
     5761 acctgaaggt aaagctaagg ctgataagtt ttttgtagga ttttggaatg gatctgacct
     5821 tgtggctgtt atggattttg tctatgcaca tcctgatgag gagactgttt ttattggttt
     5881 gtttatggtt gatcaagcct atcagaggaa agggattggt agtcatattg tgacagaagc
     5941 actagcttat tttgctaaga actttcgaaa ggcacgtttg gcttatgtta agggaaatcc
     6001 gcaatctcag catttttggg aaaagcaggg ctttaaatca attggatgcg aggttaagca
     6061 agaactctat acggttgtta tcgttgaaca gagcctagaa gattagaaat ggcatcaagt
     6121 aagaactatt tggaatttgt tttggaacaa ttatcaggat tagatgatgt gacttaccgt
     6181 tctatgatgg gggagtatat tctttacttc cgcggcaaga ttattggcgg catttatgac
     6241 gatcgctttt tagttaaacc cgtgcaagca gtcttagata agattgacca atcttctttt
     6301 gagtttccat acaaaggtgc caaagaaatg atttgagtgg aagaacttga taataagatg
     6361 tttctataag acctgatttt agctatgtat aaccaactgc caacgcccaa acctaaaaag
     6421 aaaaagcaag ggtgaacgaa gtaaaaaaga agtctgctaa ggccctgtct ttgcacgggt
     6481 aaaattttat atataaaaag aagctgggac taaagagctc agcttccttt ggtttatata
     6541 attgtcatta caagacgaag tggttgggcg aaactctgtt gactttattc aatttagagt
     6601 ttcttatgca caattgagtc tggaacgaaa gtctccagtt gcaaagtata cagtacaata
     6661 aaccaacgat gtaatagctg atgacacaaa gcacagtggg taggacttgt gaagtcaccc
     6721 ttttcttttc aaaatttata ctaaatcatt gatatcagtg tagtcacgat taagtccttg
     6781 agcaactggt aggctagtca agtaaccttg ataagtggtc acaccttgac gcaagccttc
     6841 atcttcagag attgcttgtg cgaatccttt gccagccaaa gcttcgatat aaggaagagt
     6901 gacattggtt agggcgatgg ttgaagtgcg ggcaaccgca ccagggatat tggcaacggc
     6961 atagtggaga acaccgtgtt tttcatagac gggttcatcg tgcgttgtca cacggtcagc
     7021 tgtttcgata acgccacctt ggtcaacagc aacgtcaacg atacagagcc tggacgcatt
     7081 tgtttgacca tctcatctgt caccaattcc ggtgcttttg caccagggat gagaatggct
     7141 ccaatcacca catcagcatc tctcacactt gcttcaatgt tgaatgaatt agacataaga
     7201 gtttgaattt gacttccaaa gacttcttct agaactgaga gaagcttggc actaatatct
     7261 aaaatagtca cttgagcacc aagaccaagg gcgatgcggg cagcatgtgt accgacgaca
     7321 ccatcaccga tgatagttac ttttcctttt ggaacaccag agccaccagc ttgcttagta
     7381 aggaagtgag ctccgatttg aacagccata cgacctgcaa cctcactcat aggaacgagg
     7441 agcggtagtt gtccttgatt gtcacgaaca gtttcatagg caattcctgt tgtttttgct
     7501 gctaacatag catctgctaa ttctggagca gcggccatgt gcaagtaggt gaagagaaga
     7561 agatcgtcgc gcaagtaacc gtattcagaa cttaaaggtt cttttacttt cacaaccaac
     7621 tctgctgccc aagcttcacc agcagtagcg acaatctcag ctccttgctt ttgatagtca
     7681 gcatcagtaa agccagaacc gagaccagca tttgtttcga taaggacacg atgaccacga
     7741 ctaactaagc tatgaacacc tgcaggtgtg agggcgacac ggttttcgtt atttttaatt
     7801 tcttttggga ttccgattaa cattgagata acctaccttt caattgacgg tcttgttttg
     7861 gttgtcacat tcgagttcat aaatcaaaaa tgtgacggtt tcattgtata tgaaaccgct
     7921 tcaaaaatca agaaaaactt gtcatccaaa tttttttatg ctagactagt gaaaatcaag
     7981 ctctaatgga gggaaaagta tggaatcaat atttgtgaaa tttgcccagt atccgtctat
     8041 agaaacggag cgtttattgc ttagacctgt aactttggat gatgcggaag caatgtttga
     8101 ctatgcctcg gacaagggta atacacgtta cacttttcca accaatcaaa gcttggaaga
     8161 aaccaagaat aacattgctc agttctactt ggctaatccc ttgggacgtt gggggattga
     8221 attaaaagat aacggcaagt ttatcggaac tattgactta cacaagattg atcctgttct
     8281 taagaaggca gctattggct acattatcaa taaaaagtat tggaatcaag gattaacgac
     8341 agaagccaat cgtgctgtga ttgagctagc ttttgagaag atagggatga ataagttgac
     8401 tgcccttcac gataaggcta atcccgcgtc aggaaaggtc atggagaaat caggcatgcg
     8461 tttttcccat gcagaaccat atgcttgtat ggaccagcat gaaaaaggcc gaatcgtgac
     8521 aagagttcat tatgtcttga ccaaggaaga ctattttgca aataaataag cagttgaaaa
     8581 gaaatttttc gactgttttt tcttcctctt acgaataatc taagagagga gaaaatatgg
     8641 aagcaattat cgagaaaatc aaagagtata aaatcatcgt catctgtact ggtctgggct
     8701 tgcttgtagg aggatttttc ctgctaaagc cagctccaca aacacctgtc aaagagacga
     8761 atttgcaggc tgaagttgca gctgtttcca aggactcatc gaccggaaag gaagtgaaca
     8821 aggaagagaa ggaagaaccc cttgaacaag atctaatcac agtagatgtc aaaggtgctg
     8881 tcaaatcgcc agggatttat gacttgcctg taggtagtcg agtcaatgat gctgttcaga
     8941 aggctggtgg cttgacagag caagcagaca gcaagtcgct caatctagct cagaaagtta
     9001 gtgatgaggc tctggtttac gttcctacta agggagaaga agcagttagt caacagactg
     9061 gttcggggac agcttcttca acaagcaagg aaaagaaggt caatctcaac aaggccagtc
     9121 tggaagaact caagcaggtc aagggactgg gaggaaaacg agctcaggac attattgacc
     9181 atcgtgaggc aaatggcaag ttcaagtcag tagacgagct caagaaggtc tctggcattg
     9241 gtgccaagac catagaaaaa cttaaagact atgttacagt ggattaagaa tttctctatt
     9301 cccctaattt acctgagttt tctgttacta tggctttact acgccatttt ctcagtatcc
     9361 tattttgctt tgttgggctt tgtttttctg ctagtctgtc tctttttcca atttccttgg
     9421 aaatctgcta gcaaagttct agtgatttgt ggaatctttg gattttggtt tgtttttcaa
     9481 aattggcaac agagtcaagc gagtcaaaat ctggcggatt ctgttgaagg ggtacggatt
     9541 ttgcctgata ctattaaggt taatggtgat agtctatcct ttcgtggcaa gtctaacggt
     9601 cgtgctttcc aagtctatta taaactccag tccgaggagg agaaagaagc ctttcaagct
     9661 ttaaccgacc tgcatgagat aggactagaa gggaagcttt cggagccaga agggcagaga
     9721 aattttggtg gctttaatta ccaagcctat ctgaagactc agggaattta ccagactctc
     9781 aatatcaaaa caatccagtc acttcaaaag attggcagtt gggatatagg agaaaacttg
     9841 tccagtttac gtcgaaaggc tgtggtttgg attaagacgc actttccaga ccctatgcgc
     9901 aattacatga caggactctt gctgggacat ctggacaccg actttgagga gatgaatgag
     9961 ctttattcca gtctaggaat tatccacctc tttgccctat ctggcatgca ggtaggtttt
    10021 ttcatgaatg gatttaagaa acttctcttg cgattgggct tgacccaaga aaagttgaaa
    10081 tggctgactt atcccttttc ccttatctat gcgggactaa ctggattttc agcatcggtt
    10141 attcgcagtc tcttgcaaaa gctactggct caacatgggg ttaagggctt ggataatttt
    10201 gccttgacgg tgcttgtcct ctttattgtc atgccaaact ttttcttgac agcaggagga
    10261 gtcttgtcct gcgcttatgc ttttatcctg accatgacca gcaaagaagg ggaggggctc
    10321 aaggctgttg ctagtgaaag tctagtcatc tccttgggca tattgcccat tctatccttc
    10381 tattttgcgg aatttcaacc ttggtctatc cttttgacct ttgtcttttc ctttctattt
    10441 gacttgacct tcttaccgct cttgtctatt ttatttgtcc tttcctttct ctatccagtc
    10501 attcagctga actttatctt tgaatggtta gagggcatta ttcgcttggt ctcgcaggtg
    10561 gcaaggagac cgcttgtctt tggtcaaccc aacgcatggc ttttaatctt attgttaatt
    10621 tccttggctt tggtctatga tttgaggaaa aacattaaag gattaacagt attgagttta
    10681 ttgattacag gtctcttttt ccttaccaag tatccactgg aaaatgaaat caccatgctg
    10741 gatgtggggc aaggagaaag tattttccta cgggatgtaa ctgggaaaac cattctcata
    10801 gatgtaggtg gtaaggcaga atcttataag aaaatcgaaa aatggcaaga aaagatgacg
    10861 accagcaatg cacagagaag cttgataccc tatcttaaaa gtcgaggagt agctaagatt
    10921 gaccagctga ttttgacaaa tacggacaag gaacatgtcg gagatttgtt agaggtgacc
    10981 aaggctttcc atgtagggga gattttagta tcaaaaggca gtttgaagca gaagcaattt
    11041 gtggtagaac tacaggcgac tcaaacaaag gtgcgtagta tgacagtagg ggagaacttg
    11101 cccatttttg gaagtcagtt agaagttcta tctctaagga aaatgggaga tggaggacac
    11161 gatgataccc tagttttgta tgggaaattc ttggataagc aatttctctt cacgggaaat
    11221 ttggaggaga aaggagagaa ggacttgctg aagcactatc cagacttgaa agtaaatgtt
    11281 ttgaaagcta gccaacatgg caataaaaaa tcatcaagtc caacctttct agaaaaactc
    11341 aaaccagagc ttactcttat ctcagttgga aagagcaatc gaatgaaact cccccatcag
    11401 gaaacattga cacgactgga aggtatcaat agcaaagttt atcgaactga ccagcaagga
    11461 gctatacgtt ttaaggggtt ggatagttgg aaaatcgaaa gtgttcgata ggaaagataa
    11521 atgttgtaga ttagtgaaat aaactaaaaa tttgttgcat aataatgata aaaatggtat
    11581 aatgaaaacg tattcaatat tgaggatata aaatcattaa aaatcagcaa aagttatttt
    11641 attagttagt ttataatcta ttggtcttct tcagtccagt gtatctgcta agagtgttct
    11701 taaaagtatc ggtatgattg gaatatcttc tataagtcta gtatgaattt ccatggatat
    11761 agacatacta acgttccgtc atggtctcgt tattacagct attctgagta tccagttggt
    11821 tctggctgga actacggttg ttatgagatc ataaactatt acagcggagg ctattaattc
    11881 ttaaagagtg agaaaaagga gggctagata tgttgcagct tactcatgtg accttaaaaa
    11941 cgcgacaagt catcttgcaa gatgcggatt tcacctttaa aaagggtagg ttttatggtc
    12001 ttcttgctat caatggctct ggaaagacga ccctgttccg tgccattagc aatttaattc
    12061 ccataagtag tggaaatatc gcaactcctc cttctttatt ttattatgag agtattgaat
    12121 ggctggatgg aaacttaagt gggatggact accttcgtct tatcaaaaac atctggaagt
    12181 cagacctaaa cttgagagat gaaatcgcct actgggaaat ggctgactat atcagtcttc
    12241 ccatccgcaa gtattcctta gggatgaagc aacgcttggt gattgctatg tattttctca
    12301 gtcaggccaa atgctggctc atggatgaga ttacaaatgg cttagacgag tattatcgac
    12361 agaagttttt tgataggcta gcacaaatcg atagacaaga acagctggtt cttttaagtt
    12421 cccactataa ggaagagttg gttgatatct gcgatagagt agtaaccatt catcaggggc
    12481 agatagaaga ggtttagttt atgaaagatg ttagtctatt tttattgaaa aaagttttca
    12541 aaagtcgttt aaactggatt atcttacttt tatttgcatc tgtactcggt gttacctttt
    12601 atttaaatag tcagactgca aactcacaca gcttggagag caggttggaa agtcgcattg
    12661 cagccaacga gagagccatc aatgaaaatg aagcgaaact ttcccaaatg tctgacacca
    12721 gctcggagga ataccagttt gctaaaaata atttagacgt gcaaaaaaat cttttgacgc
    12781 gaaagacaga aattctaact ttactaaaag aagggcgctg gaaagaagcc tactatctgc
    12841 agtggcaaga tgaagagaag aattatgaat ttgtatcaaa tgacccgact gctagctctg
    12901 gcttaaaaat gggggttgac cgcgaacgga agatttacca agccctgtat cccttgaaca
    12961 taaaagcaca tactttggag tttccgaccc acgggattga tcagattgtc tggattttag
    13021 aggctatcat cccaacctta tttgtgattg gtattatttt tatgctaaca caactatttg
    13081 cagaaagata tcaaaatcat ctggacacag ctcacttata tcctgtttca aaagtgacat
    13141 ttgcaatatc ctctcttgga gttggagtgg gatatgtaac tgtgctgttt atcggaatct
    13201 gtggcttttc ttttctagtg ggaagtctga taagtggttt tggacagtta gattatccct
    13261 actcaattta tagcttagtg aatcaagaag taactattgg gaaaatacaa gatgtattat
    13321 ttcctggctt actcttagct ttcttagcct ttatcgtcat tgtggaagtt gtgtacttga
    13381 ttgcttactt tttcaagcaa aaaatgcctg tcctctttct ttcactcatt gggattgttg
    13441 gcttattgtt tggcatccaa acaattcagc ctcttcaaag gattgcacat ctgattccct
    13501 ttacttactt gcgttcagtg gagattttat ctggaagatt atctaagcag attgataatg
    13561 tcgatctaaa ttggagcatg gggatggtct tacttccttg cctgattatc cttttgttag
    13621 tggggattct atttatcgaa agctggggaa gttcacgtaa aaaggaagtt tttaaagttt
    13681 agttttccta tagggaagct aaagaaaaac taacataggg agagaatcaa cttggttctc
    13741 tctttttgat tcgaaaacca aaccaaaata caaacacaaa cttttcaaaa aataactttt
    13801 tatcttgaca agagctagaa aacttggtat catataaaag ttgagaaaag cagaagtgag
    13861 agcttctcgc cttgtgacat taagttgcct ggccctacgg atgaaaagtt tcgaagaaac
    13921 gctatcataa cgtgcgggct tgtatattta cgagtccgct attgtttttc tctaataaaa
    13981 caaaagaggt gaaaaccata gcaaagcaag acttattcat caatgatgag attcgtgtac
    14041 gtgaagttcg cttgattggt cttgaaggag aacagctagg tatcaagcca ctcagtgaag
    14101 cgcaagcttt ggctgataac gctaatgttg acctagtatt gattcaaccc caagccaaac
    14161 cgcctgttgc aaaaattatg gactacggta agttcaaatt tgagtaccag aagaagcaaa
    14221 aagaacaacg taaaaaacaa agcgttgtta ctgtgaaaga agttcgtcta agtccaacaa
    14281 ttgacaaggg tgactttgat acaaaacttc gtaatgcacg taagttcctt gaaaaaggaa
    14341 ataaagttaa ggtatctatt cgctttaagg gtcgtatgat tacccataaa gagattggtg
    14401 caaaagtttt agccgagttt gctgaagcaa ctcaagatat tgccatcatc gaacaacgtg
    14461 ctaaaatgga tggacgtcaa atgttcatgc aattggcgcc agcgactgac aaaaaataat
    14521 tgtcagaaag ttaaataaag gagaaaaaat catgccaaaa caaaaaacac accgcgcatc
    14581 agctaaacgt ttcaaacgta caggttctgg tggacttaaa cgtttccgtg cttacacttc
    14641 tcaccgtttc cacggaaaaa ctaagaaaca acgtcgtcat cttcgtaaag catctatggt
    14701 gcattcagga gattacaaac gtatcaaagc aatgcttact cgcttgaaat aatagcttga
    14761 ctgtaagtaa acaattctag gaaatatttg gaggaaataa aacatggcac gtgttaaagg
    14821 tggcgttgta tcacgcaaac gtcgtaaacg tattcttaaa ttagcaaaag gttactatgg
    14881 agctaaacac atcttgttcc gtactgcaaa agaacaagta atgaactctt actactatgc
    14941 ataccgtgac cgtcgtcaga aaaaacgtga cttccgcaaa ttgtggatta ctcgtatcaa
    15001 tgcggcagct cgtatgaacg gactttcata ctcacaattg atgcatggtt tgaaattggc
    15061 tgagattgaa gttaaccgta aaatgcttgc tgacttggct gttaacgatg cagtagcttt
    15121 cacagctctt gcagatgcag ctaaagcaaa acttggtaaa taacaactga tgagactgga
    15181 acaggattgt ccagtctttt taaaaataaa ggagaaagat atggcttcaa aaatgctaca
    15241 cacttgctta cgagtagaaa atcttgaaaa atcaatcgca ttttatcaag atgcttttgg
    15301 ttttaaagaa ttgcgtcgca gagattttcc agaccatgcc ttcacgattg tctatctagg
    15361 tcttgagggt gatgactatg agttggagtt gacttataac tacgatcacg gtccgtatgt
    15421 ggttggtgat gggtttgccc atatcgccct cagtacacct gatcttgagg cacttcatca
    15481 agaacacagt gcaaaaggct atgaagtgac tgagccaaat ggtctaccag gaactacacc
    15541 aaactattac tttgtcaaag accctgatgg atataaggtc gaagtcattc gtgaaaaata
    15601 atttcgtgca agttaagtag aatattcgtt ttctacttga ctttttttag ctgaaagagt
    15661 atactaataa aaattcaacc tttaagggga gtccagagag actcacaagg tgtcagataa
    15721 aagaatagtg caattttcta gaggagactt tttgagtgtg ctctcttgtg ttgtacgatt
    15781 ttaactgagg ccttgcacta gcaaggtctt ttctttatct ggtcccctta aaatttaagg
    15841 aggaaaagtt atgaatccca catgtaagaa gcgtttgggt gtcattcggt tggaaaccat
    15901 gaaggtggtt gcacaagagg aaatcgcgcc agcaatcttt gaattagtcc tagaaggaga
    15961 aatggttgaa gccatgcgag caggccaatt tcttcatctg cgtgtaccgg acgatgccca
    16021 tctcttacgt cgtcctattt caatttcgtc tattgacaag gcaaacaagc agtgtcacct
    16081 catttatcgg attgagggag ctgggactgc aattttttca accttaagtc agggagacac
    16141 tcttgatgtg atggggcctc agggaaatgg ttttgacttg tctgaccttg atgagcagaa
    16201 tcaggttctc cttgttggtg gtgggattgg tgttccaccc ttgcttgagg tggccaagga
    16261 attgcatgaa cgtggagtga aagtagtgac agtcctcggt tttgctaata aggatgctgt
    16321 tattttgaaa acggaattgg ctcagtatgg tcaggtcttt gtaacgacag atgatggttc
    16381 ttatggcatc aagggaaatg tttccgttgt tatcaatgat ttagatagtc agtttgatgc
    16441 tgtttactcg tgtggggctc caggaatgat gaagtatatc aatcaaacct ttgataatca
    16501 cccaagagcc tatttatctc tggaatctcg tatggcttgt gggatgggag cttgctatgc
    16561 ctgtgttcta aaagtaccag aaaacgagac ggtcagccaa cgcgtctgtg aagatggtcc
    16621 tgttttccgc acaggaacag ttgtattata aggagaaaat tatgactaca aatcgattac
    16681 aagtttctct acctggtttg gatttgaaaa atccgattat tccagcatca ggctgttttg
    16741 gctttggaca agagtatgcc aagtactatg atttagacct tttaggttct attatgatca
    16801 aggcgacaac ccttgaacca cgttttggga atccaactcc aagagtggca gagacgcctg
    16861 ctggtatgct caatgcaatt ggcttgcaaa atcctggttt agaggttgtt ttggctgaaa
    16921 agctaccttg gctggaaaga gaatatccaa atcttcttat tattgccaat gtagctggtt
    16981 tttcaaaaca agagtatgca gctgtttctc atgggatttc caaggcaact aatgtaaaag
    17041 ctatcgagct caatatttct tgtcccaatg ttgaccactg taatcatgga cttttgattg
    17101 gtcaagatcc agatttggct tatgatgtgg tgaaagcagc tgtggaagcc tcagaagtgc
    17161 cagtttatgt caaattaacc ccgagtgtga ccgatatcgt tactgtcgca aaagctgcag
    17221 aagatgcggg agcaagtggc ttgaccatga tcaatactct ggttggaatg cgctttgacc
    17281 tcaaaaccag aaaaccaatc ttggccaatg gaacaggtgg aatgtcaggt ccagcagtct
    17341 ttccagtagc cctcaaactc atccgccaag tagcccaaac aacagacctg cctatcattg
    17401 gaatgggggg agtggattcg gctgaagctg ccctagaaat gtatctggct ggggcatctg
    17461 ctatcggagt tggaacagct aactttacca atccttatgc ctgccctgac atcatcgaaa
    17521 atttaccaaa agtcatggat aaatacggta ttagcagtct ggaagaactc cgtcaggaag
    17581 taaaagagtt tctgaggtaa actgcaatca atctgttctt gattttttat tagtttgtaa
    17641 tatgaattta ggagaatttt ggtacaataa aataaataag aacagaggaa gaaggttaat
    17701 gaagaaagta agatttattt ttttagctct gctatttttc ttagctagtc cagagggtgc
    17761 aatggctagt gatggtactt ggcaaggaaa acagtatctg aaagaagatg gcagtcaagc
    17821 agcgaatgag tgggtttttg atactcatta tcaatcttgg ttttatataa aagcagatgc
    17881 taactatgct gaaaatgaat ggctaaagca aggtgacgac tatttttacc tcaaatctgg
    17941 tggctatatg gccaaatcag aatgggtaga agacaaggga gccttttatt atcttgacca
    18001 agatggaaag atgaaaagaa atgcttgggt aggaacttcc tatgttggtg caataggtgc
    18061 caaagtaata gaagactggg tctatgattc tcaatacgat gcttggtttt atataaaagc
    18121 agatggacag cacgcagaga aagaatggct ccaaattaaa gggaaggact attatttcaa
    18181 atccggtggt tatctactga caagtcagtg gattaatcaa gcttatgtga atgctagtgg
    18241 tgtcaaagta cagcaaggtt ggctttttga caaacaatac caatcttggt tttacatcaa
    18301 agaaaatgga aactatgctg ataaagaatg gattttcgag aatggtcact attattatct
    18361 aaaatccggt ggctacatgg cagccaatga atggatttgg gataaggaat cttggtttta
    18421 tctcaaattt gatgggaaaa tagctgaaaa agaatgggtc tacgattctc atagtcaagc
    18481 ttggtactac ttcaaatccg gtggctacat ggcagccaat gaatggattt gggataagaa
    18541 atcttggttt tacctcaagt ttgatggcaa aatggctgaa aaagaatggg tttacgattc
    18601 tcatagtcaa gcctggtact acttcaaatc cggtggttac atgacagcca atgaatggat
    18661 ttgggataag gaatcttggt tttacctcaa gtctgatggc aaaatggctg aaaaagaatg
    18721 ggtctacgat tctcatagtc aagcctggta ctacttcaaa tctggtggct acatggcgaa
    18781 aaatgagaca gtagatggtt atcagcttgg aagcgatggt aaatggcttg gaggaaaagc
    18841 tacaaataaa aatgctgctt actatcaagt agtgcctgtc acagccaatg tttatgattc
    18901 agatggtgaa aagctttcct atatatcgca aggtagtgtc gtatggctag ataaggatag
    18961 aaaaagtgat gacaagcgct tggctattac tatttctggt ttgtcaggct atatgaaaac
    19021 agaagattta caagcgctag atgctagtaa ggactttatc ccttattatg agagtgatgg
    19081 ccaccgtttt tatcactatg tggctcagaa tgctagtatc ccagtagctt ctcatctttc
    19141 tgatatggaa gtaggcaaga aatattattc ggcagatggc ctgcattttg atggttttaa
    19201 gcttgagaat cccttccttt tcaaagattt aacagaggct acaaactaca gtgctgaaga
    19261 attggataag gtatttagtt tgctaaacat taacaatagc cttttggaga acaagggcgc
    19321 tacttttaag gaagccgaag aacattacca tatcaatgct ctttatctcc ttgcccatag
    19381 tgccctagaa agtaactggg gaagaagtaa aattgccaaa gataagaata atttctttgg
    19441 cattacagcc tatgatacga ccccttacct ttctgctaag acatttgatg atgtggataa
    19501 gggaatttta ggtgcaacca agtggattaa ggaaaattat atcgataggg gaagaacttt
    19561 ccttggaaac aaggcttctg gtatgaatgt ggaatatgct tcagaccctt attggggcga
    19621 aaaaattgct agtgtgatga tgaaaatcaa tgagaagcta ggtggcaaag attagtacta
    19681 taagtgaata tgatttgagt gaatagtaag ttaaaaatcc tgatttcaag taaaatcagg
    19741 attttttcat ggatgcaatt tttttggagt ctggtgtgac gcggagggtc ttttgtcctg
    19801 tgtaagtgac aaagccgggt tttccaccag ttggtttatt gagttttttg acttcaatca
    19861 tatctacctg caccagattc gacaggcgcc cttgagagaa gtaggcagct aactctgctg
    19921 cgtctgtctt gactgcatca gatgggtcaa gatttcctga gatgacaaca tggcttccag
    19981 gaatgtcctt agcatggaac caaagttcct ccttgcgggc cattttaaag gtcaattcct
    20041 cattttgaag attgtttcgt ccgacataga tgatggtttt gccatcgctt gctagatatt
    20101 gttctagttt tttgcgtttc tggattttct cccgttgtct tctgcggata aaacctgttt
    20161 gaatcaattc ttcacggatt tcagcgattt cttccagtcc agcttggttg aggacggttt
    20221 ctacactttc cagatagaga atagtggctt tggtttcttc aatcaaatca gtcaagtatt
    20281 tgacagcttc tttgagtttc tgataccgtt taaaatagcg ttgggcattc tggttgggag
    20341 tcagagcctt atcaagcgca atcatgatag gttggttggt atagtagttg tctaggataa
    20401 cctggtcttg gtcgttaggc acttggtgga ggaaggttgt cagcaattct cctttttgac
    20461 gaaattcttc agcgttgtct gtcgccagta actctttttc ctgttttttg agtttgtgtc
    20521 ggtttttctg aagttcattt tcaacacgac gaatcagttc actggcctgc tgtttgacgc
    20581 ggtcgcgctc agccttatcc ttatagtagg tgtccaacaa atcagaaaga tttgcaaaag
    20641 gctctcccac ctgatttgca aaaggaactg gactgaagga agtctcagtc aagcatggct
    20701 tggtttcctg attgaaaaaa tttcggaaag cggaaagttt ttcactaacc agtatccttt
    20761 ccaattcatt tgccgtatcg cgtcccagac cttgaaagag gctttgaaga ttttttgctg
    20821 ttagttcttg ggtttgcagg atttcaaaga gcttttcatc cttgatagta aaaggattga
    20881 gagattctgt acttggcgga gcgatatagg tcgatcctgg aagtaaggtg cggtagctat
    20941 tttgtgaaaa gccgacgtgt ttgataactt cgaggatttt atgactgctt ttatcgacca
    21001 gtagaatatt actgtgtttc cccataattt cgataatcaa ggtagcctgg atatggtctc
    21061 caatctcgtt tttattggaa actgtcattt ccacaatacg gtcattttcc acttgctcaa
    21121 tcgactcaat cagggccccc tgcaaatact ttctcaaaac catgataaag gtagaaggtt
    21181 gggctggatt ttcaaaagtc gtttgggtca gctgaatgcg tccaaaaact ggatgggcag
    21241 aaaggagcag gcgatggctt tggcgattgc tgcggatttg caagaccaac tcttgttcaa
    21301 aaggctgatt gattttctgg atgcgaccat tcactaattc gcttcgcaat tcctcaacta
    21361 tgtggtgtaa aaaaaatccg tcaaatgaca tcgttctctc cttgtgattg tattccatag
    21421 tattatatca aaaaggtaga ataaaatcat ggaaatgtgg tataataaag ccaagtaaag
    21481 agaaacgaga agcacatgta tattgaaatg gtagatgaaa ctggtcaagt ttcaaaagaa
    21541 atgttgcaac aaacccaaga aattttggaa tttgcagccc aaaaattagg aaaagaagac
    21601 aaggagatgg cagtcacttt tgtgaccaat gagcgtagtc atgaacttaa tctggagtac
    21661 cgtgacaccg accgtccgac agatgtcatc agccttgagt ataaaccaga attggaaatt
    21721 gcctttgacg aagaggattt gcttgaaaat ccagaattgg cagagatgat gtctgagttt
    21781 gatgcctata ttggggaatt gttcatctct atcgataagg ctcatgagca ggccgaagaa
    21841 tatggtcaca gctttgagcg tgagatgggc ttcttggcag tacacggctt tttacatatt
    21901 aacggctatg atcactatac tccggaagaa gaagcggaga tgttcggttt acaagaagaa
    21961 attttgacag cctatggact cacaagacaa taaacgaaaa tggaaaaatc gtgacctgat
    22021 atccagttta gaatttgcca tcacagggat ttttactgct atcaaggaag aacgcaatat
    22081 gcgaaaacat gcagtgacgg ctctagtggt catccttgca ggttttggtt ttcaggtgtc
    22141 acgaatcgaa tggctctttc tcctattgag cattttcttg gtggtagcct ttgaaattat
    22201 caattctgct atcgaaaatg tggtggattt agccagtcac tatcactttt ccatgctggc
    22261 taaaaatgcc aaggatatgg cggctggcgc ggtattagtg gtttctcttt ttgcagcctt
    22321 aacaggcgca ttgatttttc tcccacgaat ctgggattta ttattttaaa cagtaagagg
    22381 aaattatgac ttttaaatca ggctttgtag ccattttagg acgtcccaat gttgggaagt
    22441 caaccttttt aaatcacgtt atggggcaaa agattgccat catgagtgac aaggcgcaga
    22501 caacgcgcaa taaaatcatg ggaatttaca cgactgataa ggagcaaatt gtctttatcg
    22561 acacaccagg gattcacaaa cctaaaacag ctctcggaga tttcatggtt gagtctgcct
    22621 acagtaccct tcgcgaagtg gacactgttc ttttcatggt gcctgctgat gaagcgcgtg
    22681 gtaaggggga cgatatgatt attgagcgtc tcaaggctgc caaggttcct gtgattttgg
    22741 tggtgaataa aatcgataag gttcatccag accagctctt gtctcagatt gatgacttcc
    22801 gtaatcaaat ggactttaag gaaattgttc caatctcagc ccttcaggga aataacgtgt
    22861 ctcgtctagt ggatattttg agtgaaaatc tggatgaagg tttccaatat ttcccgtctg
    22921 atcaaatcac agaccatcca gaacgtttct tggtttcaga aatggttcgc gagaaagtct
    22981 tgcatctaac tcgtgaagag attccgcatt ctgtagcagt agttgttgac tctatgaaac
    23041 gagacgaaga gacagacaag gttcacatcc gtgcaaccat catggtcgag cgcgatagcc
    23101 aaaaagggat tatcatcggt aaaggtggcg ctatgcttaa gaaaatcggt agcatggccc
    23161 gtcgtgatat cgaactcatg ctaggagaca aggtcttcct agaaacctgg gtcaaggtca
    23221 agaaaaactg gcgcgataaa aagctagatt tggctgactt tggctataat gaaagagaat
    23281 actaagtaga ggtaggctca tgcctgcttc ttgtttttac agaaggagga cttatgcctg
    23341 aattacctga ggttgaaacc gtttgtcgtg gcttagaaaa attgattata ggaaagaaga
    23401 tttcgagtat agaaattcgc taccccaaga tgattaagac ggatttggaa gagtttcaaa
    23461 gggaattgcc tagtcagatt atcgagtcaa tggaacgtcg tggaaaatat ttgctttttt
    23521 atctgacaga caaggtcttg atttcccatt tgcggatgga gggcaagtat ttttactatc
    23581 cagaccaagg acctgaacgc aagcatgccc atgttttctt tcattttgaa gatggtggca
    23641 cgcttgttta tgaggatgtt cgcaagtttg gaaccatgga actcttggtg cctgaccttt
    23701 tagacgccta ctttatttct aaaaaattag gtcctgaacc aagcgaacaa gactttgatt
    23761 tacaggtctt tcaatctgcc cttgccaagt ccaaaaagcc tatcaaatcc catctcctag
    23821 accagacctt ggtagctgga cttggcaata tctatgtgga tgaggttctc tggcgagctc
    23881 aggttcatcc agctagatct tcccagactt tgacagcaga agaagcgaca gctattcatg
    23941 accaaaccat tgctgttttg agccaggctg ttgaaaaagg cggttccacc attcggacct
    24001 ataccaatgc ctttggggaa gatggaagca tgcaggattt tcatcaggtc tatgataaga
    24061 ctggtcaaga atgtgtacgc tgtggtacca tcattgagaa aattcaacta ggtggacgtg
    24121 gaacccactt ttgtccaaac tgtcaaagga gggactgatg ggaaaaatca tcggaatcac
    24181 tgggggaatt gcctctggta agtcaactgt gacaaatttt ctaagacagc aaggctttca
    24241 agtagtggat gccgacgcag tcgtccacca actacagaaa cctggtggtc gtctgtttga
    24301 ggctctagta cagcactttg ggcaagaaat cattcttgaa aacggagaac tcaatcgccc
    24361 tctcctagct agtctcatct tttcaaatcc tgatgaacga gaatggtcta agcaaattca
    24421 aggggagatt atccgtgagg aactggctac tttgagagaa cagttggctc agacagaaga
    24481 gattttcttc atggatattc ccctactttt tgagcaggac tacagcgatt ggtttgctga
    24541 gacttggttg gtctatgtgg accgagatgc ccaagtggaa cgcttaatga aaagggacca
    24601 gttgtccaaa gatgaagctg agtctcgtct ggcagcccag tggcctttag aaaaaaagaa
    24661 agatttggcc agccaggttc ttgataataa tggcaatcag aaccagcttc ttaatcaagt
    24721 gcatatcctt cttgagggag gtaggcaaga tgacagagat taactggaag gataatctgc
    24781 gcattgcctg gtttggtaat tttctgacag gagccagtat ttctttggtt gtacctttta
    24841 tgcccatctt cgtggaaaat ctaggtgtag ggagtcagca agtcgctttt tatgcaggct
    24901 tagcaatttc tgtctctgct atttccgcgg cgctcttttc tcctatttgg ggtattcttg
    24961 ctgacaaata cggccgaaaa cccatgatga ttcgggcagg tcttgctatg actatcacta
    25021 tgggaggctt ggcctttgtc ccaaatatct attggttaat ctttcttcgt ttactaaacg
    25081 gtgtatttgc aggttttgtt cctaatgcaa cggcactgat agccagtcag gttccaaagg
    25141 agaaatcagg ctctgcctta ggtactttgt ctacaggcgt agttgcaggt actctaactg
    25201 gtccctttat tggtggcttt atcgcagaat tatttggcat tcgtacagtt ttcttactgg
    25261 ttggtagttt tctattttta gctgctattt tgactatttg ctttatcaag gaagattttc
    25321 aaccagtagc caaggaaaag gctattccaa caaaggaatt atttacctcg gttaaatatc
    25381 cctatctttt gctcaatctc tttttaacca gttttgtcat ccaattttca gctcaatcga
    25441 tcggccctat tttggctctt tatgtacgcg acttagggca gacagagaat cttctttttg
    25501 tctctggttt gattgtgtcc agtatgggct tttccagtat gatgagtgca ggagtcatgg
    25561 gcaagctagg tgacaaggtg ggcaatcatc gtctcttggt tgtcgcccag ttttattcag
    25621 tcatcatcta tctcctctgt gccaatgcct ctagccccct tcaactagga ctctatcgtt
    25681 tcctctttgg cttgggaacc ggtgccttga ttcccggggt taatgcccta ctcagcaaaa
    25741 tgactcccaa agccggcatt tcgagggtct ttgccttcaa tcaggtattc ttttatctgg
    25801 gaggtgttgt tggtcccatg gcaggttctg cagtagcagg tcaatttggc taccatgctg
    25861 tcttttatgc gacaagcctt tgtgttgcct ttagttgtct ctttaacctg attcaatttc
    25921 gaacattatt aaaagtaaag gaaatctagt gcgagtaaaa attaatctca aatgctcctc
    25981 ttgtgacagt atcaattacc taaccagtaa aaattcaaaa acccatccag acaagattga
    26041 ggttttaaag tattgtccca aggaaagaaa agtaacccta catcttgaat ctaagtagat
    26101 tttatggtaa aataataggg attttaagga gtttgatatg tataacctat tattaaccat
    26161 tttattagta ttatctgttg tgattgtgat tgcaattttc atgcaaccaa ccaaaaacca
    26221 atccagcaat gtatttgatg ccagttcagg tgatttgttt gaacgcagta aagctcgcgg
    26281 ttttgaagct gtaatgcagc gtttgacagg gattttagtc tttttctggc tagccattgc
    26341 cttagcattg acggtattat caagtagata agaaaataat gggcaggact aggtctttgc
    26401 ctctttttat ttttaaagga tgtttgagaa ggttttacag taaaagaaaa ttaaaaaatc
    26461 tagaaagaaa atatgaaaga tagaataaaa gaatatttac aagacaaggg aaaggtgact
    26521 gttaatgatt tggctcaggc tttgggaaaa gacagttcca aggattttcg tgagttgatt
    26581 aaaaccttgt ccttaatgga aagaaagcac cagattcgct ttgaagaaga tggcagtctg
    26641 accttggaag ccaagaaaaa acatgagatt accctcaagg ggatttttca tgcccataaa
    26701 aatggttttg gttttgtcag cctagaaggg gaagaggacg acctttttgt agggaaaaat
    26761 gatgtcaact atgctattga tggtgatacc gtcgaggtag tgattaagaa agtcgctgac
    26821 cgcaataagg gaacagcagc agaagccaaa attattgata tcctagaaca cagtttgaca
    26881 acagttgtcg ggcaaatcgt tctggatcag gaaaagccta agtatgctgg ctatattcgt
    26941 tcaaaaaatc agaaaatcag tcaaccgatt tatgttaaga aaccagccct aaagttagaa
    27001 ggaacagaag ttctcaaggt ctttatcgat aaatacccaa gcaagaaaca tgatttcttt
    27061 gtcgcgagtg ttctcgatgt agtgggacac tcaacggatg tcggaattga tgttcttgag
    27121 gtcttggaat caatggacat tgtatccgag tttccagaag ctgttgttaa ggaagcagaa
    27181 agtgtgcctg atgctccgtc tcaaaaggat atggaaggtc gtctggatct aagagatgaa
    27241 attaccttta ccattgacgg tgcggatgcc aaggacttgg acgatgcagt gcatatcaag
    27301 gctctgaaaa atggcaatct ggagcttggg gttcacatcg cagatgtttc ttattatgtg
    27361 accgaggggt ctgcccttga caaggaagcc cttaaccgtg cgacttctgt ttacgtgaca
    27421 gaccgagtgg tgccaatgct tccagaacga ctatcaaatg gcatctgctc tctcaatccc
    27481 caagttgacc gcctgaccca gtctgctatt atggagattg ataaacatgg tcgtgtggtc
    27541 aactatacca ttacacaaac agttatcaag accagttttc gtatgaccta tagcgatgtc
    27601 aatgatatcc tagctggcga tgaagaaaag agaaaagaat atcataaaat tgtatcaagt
    27661 atcgaactca tggccaagct tcatgaaact ttagaaaaca tgcgtgtgaa acgtggagct
    27721 ctcaattttg ataccaatga agcgaagatt ttagtggata aacaaggtaa gcctgttgat
    27781 atcgttcttc ggcagcgtgg tattgccgag cggatgattg agtcttttat gttgatggct
    27841 aatgaaacag ttgccgaaca tttcagcaag ttggatttgc cttttatcta tcgaattcac
    27901 gaggagccta aggctgaaaa ggttcagaag tttattgatt atgcttcgag ttttggcttg
    27961 cgcatttatg ggactgccag tgagattagt caggaggcac ttcaagacat catgcgtgct
    28021 gttgagggag aaccttatgc agatgtattg tccatgatgc ttcttcgctc tatgcagcag
    28081 gctcgttatt cggagcacaa tcacggccac tatggactag ctgctgacta ttatactcac
    28141 tttaccagtc caattcgtcg ttatccagac cttcttgttc accgtatgat tcgggattac
    28201 ggccgttcta aggaaatagc agagcatttt gaacaagtga ttccagagat tgcgacccag
    28261 tcttccaacc gtgaacgtcg tgccatagaa gctgagcgtg aagttgaagc catgaaaaag
    28321 gctgagtata tggaagaata cgtgggtgaa gagtatgatg cagttgtatc aagtattgtc
    28381 aaattcggtc tctttgtcga attgccaaac acagttgaag gcttgattca catcactaat
    28441 ctacctgaat tttatcattt caatgagcgt gatttgactc ttcgtggaga aaaatcaggt
    28501 atcactttcc gagtgggtca gcagatccgt atccgtgttg aaagagcgga taaaatgact
    28561 ggagagattg atttttcatt cgtacctagt gagtttgatg tgattgaaaa aggcttgaaa
    28621 cagtctagtc gtagtggcag agggcgtggt tcaaatcgtc gttcggataa gaaggaagac
    28681 aagagaaaat taggacgctc aaatgataag cgtaagcatt cacaaaaaga caagaagaaa
    28741 aaaggaaaga aaccttttta caaggaagta gctaagaaag gagccaagca tggcaaaggg
    28801 cgagggaaag gtcgtcgcac aaaataaaaa ggcacgccac gactatacaa tcgtagatac
    28861 gctagaggca gggatggtcc tgactggaac tgaaatcaag agtgtacgag ctgctcgaat
    28921 taatctcaag gatggctttg ctcaagtgaa aaatggagaa gtttggctga gtaatgttca
    28981 tatcgcgcct tacgaagagg gcaatatctg gaaccaggaa ccagaacgtc gtcgtaaact
    29041 cctgctccat aaaaagcaaa ttcaaaaatt ggaacaagag accaaaggga caggaatgac
    29101 cttagttccc cttaaggtct atataaaaga tggctacgct aagcttcttt taggacttgc
    29161 caaagggaag catgactatg acaaacggga gtctatcaaa cgtcgtgagc aaaatcgaga
    29221 tatcgcgcgt gtgatgaaag ctgttaatca gcgataaaaa gaggaattga aaatggaaaa
    29281 attagttgcc tataaacgca tgcctttgtg gaataaacaa acaatgcctg aagctgttca
    29341 gcaaaagcac aatacaaaag ttgggacttg ggggaaaatt actgtcttga agggagctct
    29401 caagtttatt gaattgacag aagaagggga agttctagct gaacacctct ttgaagcagg
    29461 ggcagacaat ccaatagccc aacctcaagc ctggcaccga gtggaagctg ccacagatga
    29521 tgtggaatgg tacttggaat tttattgtaa acctgaggat tattttgcta aaaaatacaa
    29581 taccaatcct gttcattcag aggtcctaga ggccatgcag acagtgaaac aagggaaagc
    29641 tttggatttg ggttgtggtc aggggcgtaa ttctcttttt ctagcccagc aagattttga
    29701 tgtgacggct gtagatcaaa atggactagc tcttgaaatc ttgcaaagca ttgtggagca
    29761 ggaagatttg gacatgcctg ttggccttta cgatatcaat tcagctagca ttgaacaaga
    29821 atatgatttt atcgtttcaa cagttgttct catgtttcta caagcggacc gcattccagc
    29881 tattattcaa aatatgcagg agaaaaccag tgttggtggt tacaacctta tcgtttgtgc
    29941 catggacacg gaggattatc cttgctcggt taacttccca ttcaccttta aagaaggaga
    30001 actggcagac tattacaagg attgggaatt ggttaagtac aatgaaaatc caggccattt
    30061 gcaccgtcgc gatgagaatg gcaatcgtat tcaactacgc tttgcgacct tactagctaa
    30121 gaaaatcaag taaacacaca tgaagattag gaattttcct gatctttttt cttttttacg
    30181 aatgatatag aaaaggaggg aattcatgtt tgttgcgaga gatgctaggg gagaattggt
    30241 aaatgtgtta gaggataaac ttgagaagca agcatacacc tgcccagctt gtggaggcca
    30301 gatccatttg cgtcaaggac caagtgtacg gacgcatttt gcccataaat ccttaaaaga
    30361 ctgtgatttt ttctttgaaa atgaaagtcc agaacacctg gccaataagg aatccctcta
    30421 tcactggttg aaaaaagaga caaaggttca attagagtac ccgctttcag aacttaaaca
    30481 gattgcggat gtatttgtaa atggcaatct agctctagaa gttcagtgta gtcccttgcc
    30541 tcagaaagtc cttaaagagc gaagtgaggg ctatcgtagt cagggttacc aagtactgtg
    30601 gttgctgggt caaaaactgt ggctcaagga gcgtttgact cgtctacagc aaggttttct
    30661 ttatttcagt caaaacatgg gcttttatgt ttgggaatta gacaaggaaa aacaagtttt
    30721 aagactcaaa tacctgattt accaggatct ccgcggtaaa ctccattatc aaatcaagga
    30781 attttcctat ggtcaaggta gtttattgga aatattgcgt cttccctata agagacaaaa
    30841 aatatctcat tttacagttt ctgaggacaa ggacatctgt cgctatatcc ggcaacaact
    30901 ttattatcaa aatctctttt ggatgaaaga acaagcagaa gcctatcaaa agggagaaaa
    30961 tatcctgact tatggactga aagaatggta tccacaaatt cgaccaatag tgggcaaatt
    31021 tttccagatt gaacaagact tgactagcta ttatcagcac ttttatacct attaccaaaa
    31081 aaatcctcaa aatgattggc aaaagcttta tccaccagcc ttttatcagc aatatttctt
    31141 gaaaaatatg gtagaataga aaggatggag gaatctaatg gtattacaaa gaaatgaaat
    31201 aaatgaaaaa gatacatggg atctatcaac gatctaccca actgaccagg cttgggaaga
    31261 agccttaaaa gatttaacag aacaattgga gacagtagcc cagtatgaag gccatctctt
    31321 ggatagtgcg gataacctac tagaaatcac tgaattttct cttgaaatgg aacgccagat
    31381 agagaagctt tacgcttatg cttatatgaa gaatgaccag gatacacgtg aagctaagta
    31441 tcaagagtac tatgccaagg ccatgacact ctacagccag ttagaccaag ccttttcatt
    31501 ctatgagcct gaatttatgg agattagcga aaagcagtat gctgactttt tagaagctca
    31561 accaaagctg caggtttatc aacactattt tgacaagctt ttgcaaggca aggatcacgt
    31621 tctttcacaa cgtgaagaag aattattggc tggagctgga gaaatctttg gttcagcaag
    31681 tgaaaccttc gctatcttgg acaatgcgga tattgtgttc ccttatgtcc tagacgatga
    31741 tggtaaagaa gttcagctat ctcatgggac ttacacacgt ttgatggagt ctaaaaaacg
    31801 tgaggttcgc cgtggtgcct atcaagctct ttatgcgact tacgaacaat tccaacacac
    31861 ctatgccaaa accttgcaaa ccaatgttaa ggtgcaaaat taccgtgcta aagttcgtaa
    31921 ctacaagagt gctcgtcatg cagccctcgc agcgaatttt gttccagaaa gtgtttatga
    31981 caatttggta gcagcagttc gcaagcattt gccactctta catcgctatc ttgagcttcg
    32041 ttcaaaaatc ttggggattt cagatctcaa gatgtacgat gtctacacac cgctttcatc
    32101 tgttgaatac agttttacct accaagaagc cttgaaaaaa gcagaagatg ctttggcagt
    32161 cttgggtgag gattacttga gccgtgttaa acgtgccttc agcgagcgtt ggattgatgt
    32221 ttacgaaaat caaggcaagc gttcaggtgc ctactctggt ggttcttatg ataccaatgc
    32281 ctttatgctt ctcaactggc aagacaatct ggacaatctc tttactcttg ttcatgaaac
    32341 aggtcacagt atgcattcaa gctatactcg tgaaactcag ccttatgttt acggggatta
    32401 ctctatcttt ttggctgaga ttgcctcaac taccaatgaa aatatcttga cggagaaatt
    32461 attggaagaa gtggaagacg acgcaacacg ctttgctatt ctcaataact tcctagatgg
    32521 tttccgtgga acagttttcc gccaaactca atttgctgag tttgaacacg ccattcacca
    32581 agcagatcaa aatggggagg tcttgacaag cgatttccta aataaactct acgcagactt
    32641 gaaccaagag tattatggtt tgagtaagga agacaatcct gaaatccaat acgagtgggc
    32701 tcgcattcca cacttctact ataactacta tgtatatcaa tattcaactg gctttgcggc
    32761 cgcctcagcc ttggctgaaa aaattgtcca tggtagtcaa gaagaccgtg accgctatat
    32821 cgactacctc aaggcaggta agtcggacta tccacttaat gtcatgagaa aagctggtgt
    32881 tgatatggag aaggaagact acctcaacga tgcctttgca gtctttgaac gccgtttaaa
    32941 tgagtttgaa gcccttgttg aaaaattagg attggcataa aatggttgaa tcgtatagta
    33001 agaatgctaa ccataacatg cgtcgtcctg tcgtcaaaga agaaattgta gacttgatgc
    33061 gtcagcgtca aaagcaggtc acaggtttct tgaaagaatt ggaagacttt gcccgcaagg
    33121 aaaatattcc tattattccc catgaaacgg ttgcttattt ccgttttctt atggaaacca
    33181 tgcagcctaa aaatattctg gaaattggga cggctatcgg tttttcagct ctcttgatgg
    33241 ctgaacatgc gccaaatgct aagattacaa ctattgatcg taatccagaa atgattggtt
    33301 ttgccaagga aaattttgcc cagtttgaca gtcgcaagca aatcactctc ctagagggag
    33361 atgcggtgga tgtcttatct acactgacag agtcttatga tttcgtcttt atggattctg
    33421 ccaagtctaa atacatcgtc tttctgccag aaatcctcaa acatttggaa gttggtggtg
    33481 tggttgtctt ggatgatatt tttcaaggtg gtgatgttgc caaggatatt atggaagtcc
    33541 gtcgtggtca gcgaaccatt tatcgaggcc ttcaaaaatt atttgatgca accttagaca
    33601 atccagaact caccgcaaca ttagtgcctt taggagatgg tattctcatg cttcgtaaaa
    33661 atgtagcaga tgttcaactg tctgaaagcg aatgattttc agaaaaattt aagaaaaaat
    33721 agtaaaatag atagagtaac acttatctca aaggagtaga catgaagaaa aaattattgg
    33781 caggtgccat cacactatta tcagtagcaa ctttagcagc ttgttcgaaa gggtcagaag
    33841 gtgcagacct tatcagcatg aaaggggatg tcattacaga acatcaattt tatgagcaag
    33901 tgaaaagcaa cccttcagcc caacaagtct tgttaaatat gaccatccaa aaagtttttg
    33961 aaaaacaata tggctcagag cttgatgata aagaggttga tgatactatt gccgaagaaa
    34021 aaaaacaata tggcgaaaac taccaacgtg tcttgtcaca agcaggtatg actcttgaaa
    34081 cacgtaaagc tcaaattcgt acaagtaaat tagttgagtt ggcagttaag aaggtagcag
    34141 aagctgaatt gacagatgaa gcctataaga aagcctttga tgagtacact ccagatgtaa
    34201 cggctcaaat catccgtctt aataatgaag ataaggccaa agaagttctc gaaaaagcca
    34261 aggcagaagg tgctgatttt gctcaattag ccaaagataa ttcaactgat gaaaaaacaa
    34321 aagaaaatgg tggagaaatt acctttgatt ctgcttcaac agaagtacct gagcaagtca
    34381 aaaaagccgc tttcgcttta gatgtggatg gtgtttctga tgtgattaca gcaactggca
    34441 cacaagccta cagtagccaa tattacattg taaaactcac taagaaaaca gaaaaatcat
    34501 ctaatattga tgactacaaa gaaaaattaa aaactgttat cttgactcaa aaacaaaatg
    34561 attcaacatt tgttcaaagc attatcggaa aagaattgca agcagccaat atcaaggtta
    34621 aggaccaagc cttccaaaat atctttaccc aatatatcgg tggtggagat tcaagctcaa
    34681 gcagtagtac atcaaacgaa tagtccaaat caatgagtca gggaaaaaac tcgacttcag
    34741 gaaaaaatga agcaaacatt cccacaataa aacgcatagt acaaggtttg tactgccccc
    34801 caaaaagtta gacaattaat ttatccgaag gatttagttc tgtattgcac agagctaagt
    34861 ccttttagtt ttatcttaat tctcttattg ttgtaataat caatatagtc tataatggct
    34921 cgttccaatt g
//
LOCUS       DAVHEX010000014        34315 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVHEX010000014 DAVHEX010000000
VERSION     DAVHEX010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34315
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.014"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            544..996
                     /locus_tag="VVL45_000688"
     CDS             544..996
                     /locus_tag="VVL45_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU4186893.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            1015..2352
                     /locus_tag="VVL45_000689"
     CDS             1015..2352
                     /locus_tag="VVL45_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEU4186894.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2431..3315
                     /locus_tag="VVL45_000690"
     CDS             2431..3315
                     /locus_tag="VVL45_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU4186895.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3315..4148
                     /locus_tag="VVL45_000691"
     CDS             3315..4148
                     /locus_tag="VVL45_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU4186896.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAVFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4166..6259
                     /gene="nanB"
                     /locus_tag="VVL45_000692"
     CDS             4166..6259
                     /gene="nanB"
                     /locus_tag="VVL45_000692"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HEU4186897.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISSLLLDKLSGESQTVVMKFKADKPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIDEISLFNKAISDQ
                     EVSTIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     NETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLRERHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSKVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            6271..7374
                     /locus_tag="VVL45_000693"
     CDS             6271..7374
                     /locus_tag="VVL45_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU4186898.1"
                     /translation="MVKYGVVGTGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            7548..8246
                     /locus_tag="VVL45_000694"
     CDS             7548..8246
                     /locus_tag="VVL45_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEU4186899.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            8265..9782
                     /locus_tag="VVL45_000695"
     CDS             8265..9782
                     /locus_tag="VVL45_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU4186900.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADLAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPFGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            9907..11235
                     /locus_tag="VVL45_000696"
     CDS             9907..11235
                     /locus_tag="VVL45_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU4186901.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            11487..12353
                     /locus_tag="VVL45_000697"
     CDS             11487..12353
                     /locus_tag="VVL45_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU4186902.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            12369..13208
                     /locus_tag="VVL45_000698"
     CDS             12369..13208
                     /locus_tag="VVL45_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU4186903.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            13480..13932
                     /locus_tag="VVL45_000699"
     CDS             13480..13932
                     /locus_tag="VVL45_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU4186904.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYDIDGD
                     KVFLVVQENVLNQAENDQFEYHKNYADLHLLVEGHEYSSYGSRIKDEAVAFDEASDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPNGYAGMEEKVRKYLFKILID"
     gene            14029..14373
                     /locus_tag="VVL45_000700"
     CDS             14029..14373
                     /locus_tag="VVL45_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4186905.1"
                     /translation="MVSSKYAARASFLDGQGITVDEMAWIIRGIVNALIGRYIKLGTY
                     AAKYGISMARSILSRVAATAAARVGLLTKISGWILRVAVNVADVYGNFANNIAAAWDA
                     YDKIPNNGRINF"
     gene            14650..15294
                     /locus_tag="VVL45_000701"
     CDS             14650..15294
                     /locus_tag="VVL45_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEU4186906.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLSWKEILQKGLMLASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            15463..16380
                     /locus_tag="VVL45_000702"
     CDS             15463..16380
                     /locus_tag="VVL45_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU4186907.1"
                     /translation="MSDLKKYEGVIPAFYACYDDQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGARAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTSAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKERFL"
     gene            16398..17282
                     /locus_tag="VVL45_000703"
     CDS             16398..17282
                     /locus_tag="VVL45_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU4186908.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(17298..18149)
                     /locus_tag="VVL45_000704"
     CDS             complement(17298..18149)
                     /locus_tag="VVL45_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEU4186909.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            18349..20406
                     /gene="pbp2b"
                     /locus_tag="VVL45_000705"
     CDS             18349..20406
                     /gene="pbp2b"
                     /locus_tag="VVL45_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HEU4186910.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKIVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            20417..21013
                     /gene="recR"
                     /locus_tag="VVL45_000706"
     CDS             20417..21013
                     /gene="recR"
                     /locus_tag="VVL45_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEU4186911.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            21189..22232
                     /locus_tag="VVL45_000707"
     CDS             21189..22232
                     /locus_tag="VVL45_000707"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEU4186912.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVDYDRFTVKTFFISQ
                     SGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNNITMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPKLIERLVDL
                     AKESFDKREAHLI"
     gene            22316..23689
                     /locus_tag="VVL45_000708"
     CDS             22316..23689
                     /locus_tag="VVL45_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEU4186913.1"
                     /translation="MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPTDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQALDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIRLAFQDLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSVQLHNQMILSLSPDVLDTVIFYGENIAE
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAHDQILLKGSNSMNLAKLVE
                     SLENEDK"
     gene            23676..24287
                     /locus_tag="VVL45_000709"
     CDS             23676..24287
                     /locus_tag="VVL45_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU4186914.1"
                     /translation="MKTSDFVKYLQRMIAITDTGLTFTKDPFDRERYEDLRSLLSEML
                     NQASDLDSEEVAEVLKPTSAYATPLMDVRAWIVEDEKICLVRGQGEDSWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYTKFVFGCKLLDGQFQEN
                     QEIADLQFFAIDQLPNLSEKRITKEQIELLWQVYQGHRGQYLD"
     gene            24378..25082
                     /locus_tag="VVL45_000710"
     CDS             24378..25082
                     /locus_tag="VVL45_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEU4186915.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            25301..26674
                     /gene="ftsA"
                     /locus_tag="VVL45_000711"
     CDS             25301..26674
                     /gene="ftsA"
                     /locus_tag="VVL45_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEU4186916.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            26691..27950
                     /gene="ftsZ"
                     /locus_tag="VVL45_000712"
     CDS             26691..27950
                     /gene="ftsZ"
                     /locus_tag="VVL45_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEU4186917.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            27955..28626
                     /locus_tag="VVL45_000713"
     CDS             27955..28626
                     /locus_tag="VVL45_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEU4186918.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKAAQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            28636..29175
                     /locus_tag="VVL45_000714"
     CDS             28636..29175
                     /locus_tag="VVL45_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEU4186919.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTSVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            29175..29438
                     /locus_tag="VVL45_000715"
     CDS             29175..29438
                     /locus_tag="VVL45_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEU4186920.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            29435..30220
                     /locus_tag="VVL45_000716"
     CDS             29435..30220
                     /locus_tag="VVL45_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEU4186921.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            30229..31017
                     /gene="divIVA"
                     /locus_tag="VVL45_000717"
     CDS             30229..31017
                     /gene="divIVA"
                     /locus_tag="VVL45_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HEU4186922.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLVQLAQCMKNQK"
     gene            31365..34157
                     /locus_tag="VVL45_000718"
     CDS             31365..34157
                     /locus_tag="VVL45_000718"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEU4186923.1"
                     /translation="MKLKDTLNLGKTEFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     QGKPHFTLHDGPPYANGNIHVGHAMNKISKDIIVRSKSMSGFYAPFIPGWDTHGLPIE
                     QVLSKQGVKRKEMDLVEYLKLCREYALSQVDKQREDFKRLGVSGDWENPYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPAGEARKFVVAAELLTSLSEK
                     FGWADVQVLETYRGQELNHIVTEHPWDTAVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANNLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKVKFHSEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMVAETIEHVAQLFEEHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVVVNRPELTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFALDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDTVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAQSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFETEDFVQLSELPEVQTFANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SELTIAEGPAPEAALSFEDVAFTVERAAGEVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFAEAVAEGFEEK"
BASE COUNT        10538 a         6063 c         7466 g        10248 t
ORIGIN      
        1 agaatgaaat atagagtatt tctctcttaa aagtttttgg tgaaacgaga tgtagaaagg
       61 agatttagcc aaagagtcta ttagtgctag aataatagat tagaattatt ttagaaaaac
      121 gaagtgagca gcttataaat tcaagtcccc aaatagattc atactagtat cttttgcaaa
      181 aaataaaggg cgacttcctt catgaatatc aatttcatct ataaggaagg tagctaattg
      241 aactaactta tttattctgt ttgtcgctag aaaaatcaga cctccttgtg aagattgagg
      301 agatacttaa tgaaaatcaa agaagaaact agcaagctag tagcagattg cccaaaacac
      361 cgctttgagg ttgtagataa gactgaccta tataatccaa ggtgaagcga ctgtggtttg
      421 aagagatttt caaagagtat aggctagaga gtagtgtttt tatgtccttc tagtagaaaa
      481 tgctagacag aagaatgggg aacttggata ggaaaaatag attgagaaag gaggttagaa
      541 gagatgatta ttacaaaaat tagccgttta ggaacttatg tgggagtaaa tccacatttt
      601 gcaacattaa tagattttct agaaaaaaca ggactagaaa atttaacaga aggttcgatt
      661 gctatcgatg gtaatcgatt gtttgggaat tgctttactt atctagcaga tggtcaagca
      721 ggggctttct ttgaaaccca ccaaaaatat ttggatattc atttagtttt ggaaaacgaa
      781 gaagccatgg ctgttacatc gccggaaaat gtaagcgtta cccaagaata tgatgaagag
      841 aaagatattg aattatacac agggaaagtg gaacagttgg ttcatttgag agctggcgaa
      901 tgcctcatca cttttccaga agatttacat caacccaagg ttcgtataaa tgatgaacct
      961 gtgaaaaaag ttgtctttaa agttgcgatt tcttaatgta gaaagagaag aacgatgaaa
     1021 aaaatgagaa agtttttatg tctagctgga attgcgctag cggctgttgc cttggtagct
     1081 tgttcaggaa aaaaagaagc tacaactagt actgaaccac caacagaatt atctggtgag
     1141 attacaatgt ggcactcctt tactcaagga ccccgtttag aaagtattca aaaatcagca
     1201 gatgctttca tgcaaaagca tccaaaaacg aaaatcaaga ttgaaacatt ttcttggaat
     1261 gacttctata ctaaatggac tacaggttta gcaaatggaa atgtgccaga tatcagtaca
     1321 gctcttccta accaagtaat ggaaatggtc aactcagatg ctttggttcc gctaaatgat
     1381 tctatcaagc gtattggaca agataaattt aacgaaactg ccttaaatga agcaaaaatc
     1441 ggagatgatt actactctgt tcctctttat tcacatgcac aagtcatgtg ggttagaaca
     1501 gatttgttaa aagaacataa tattgaggtt cctaaaactt gggatcaact ctatgaagct
     1561 tctaaaaaat tgaaagaagc tggagtttat ggcttgtctg ttccgtttgg aacaaatgac
     1621 ttaatggcaa cacgtttctt gaacttctac gtacgtagtg gtggaggaag cctcttaaca
     1681 aaagatctta aagcagactt gacaagccaa cttgctcaag atggtattaa atactgggtt
     1741 aaattgtata aagaaatctc acctcaagat tctttgaact ttaatgtcct tcaacaagct
     1801 accttgttct atcaaggaaa aacagcattt gactttaact ctggcttcca tatcggagga
     1861 attaatgcca acagtcctca attgattgat tcgattgatg cttatcctat tccaaaaatc
     1921 aaagagtctg ataaagacca aggaattgaa acctcaaaca ttccaatggt tgtttggaaa
     1981 aattcaaaac atccagaagt tgctaaagca ttcttagaag cactttataa tgaagaagac
     2041 tacgttaaat tccttgattc aactccagta ggtatgttgc caactattaa ggggattagc
     2101 gattctgcag cctataaaga aaatgaaact cgtaagaaat ttaaacatgc tgaagaagta
     2161 attactgaag ctgttaaaaa aggtactgct attggttatg aaaatgggcc aagtgtacaa
     2221 gctggtatgt tgactaacca acacattatt gaacaaatgt tccaagatat cattacaaat
     2281 ggaacagatc ctatgaaagc agcaaaagaa gcagaaaaac aattaaatga tttatttgag
     2341 gctgttcagt agatgtaaaa gactagaaaa taggtgggat agtgagctga aaagctctag
     2401 cccaatcttg taaaagaagg gagaaggaga atggttaaag aacgtaattt aactcgctgg
     2461 atatttgttt tgccagctat gattatcgta ggattactct ttgtttatcc gtttttctcg
     2521 agtatttttt atagctttac caataagcat ttgattatgc ctaattataa atttgttggt
     2581 ttggctaact ataaagctgt gctatcagat cccaacttct ttaatgcgtt ctttaattca
     2641 attaagtgga ccgttttctc attagttggt caagttttag tagggtttgt attggcttta
     2701 gctcttcaca gagtacgcca cttcaagaaa ttatatagga cattattgat tgttccttgg
     2761 gcatttccta ccatcgttat tgccttctct tggcagtgga ttctaaacgg ggtttatggc
     2821 tacttaccta atctaatcgt aaaattaggt ttaatggaac atacacctgc atttttgaca
     2881 gatagtacat gggcattcct atgtttggtg tttatcaaca tttggtttgg agcaccaatg
     2941 attatggtta atgtgctttc agctttgcaa acagtaccag aagaacaatt tgaggctgct
     3001 aagatagatg gtgcttcaag ttggcaggtg ttcaagttta tcgtctttcc acatattaaa
     3061 gtggttgtag gacttctagt tgttttgaga actgtatgga tctttaataa ctttgacatt
     3121 atctacctca ttactggtgg tggaccagcc aatgctacaa cgacgcttcc aatttttgct
     3181 tacaacctgg gctggggaac taaattgttg ggtcgtgctt cagcagttac agtactgctc
     3241 tttatcttct tggtggcgat ttgctttatc tactttgcta tcatcagtaa gtgggaaaag
     3301 gagggtagaa aataatgaag aagaaatcca gtatttattt agatattctc tcacatgtac
     3361 ttttagttgg tgcgaccatc gttgcagttt tcccattggt atggattatc atatcttctg
     3421 tcaaagggaa aggggaatta actcagtatc caacacgatt ttggcctgaa cagtttacat
     3481 tagattattt cactcatgtt atcaacgatt tgcacttcat tgataacatt cgaaacagtt
     3541 taatcattgc cttggctaca acccttattg cgattattat ttctgctatg gcagcctatg
     3601 gtattgttcg attctttcct aaattgggag caatcatgtc gagactactc gtcattacct
     3661 acattttccc accaattttg ttagcaattc cctattcaat tgccattgct aaagttgggt
     3721 taacaaatag tttatttggc ttgatgatgg tttatctatc ttttagtgtt ccatatgcag
     3781 tttggctctt agttggattt ttccaaacag ttccaattgg aattgaagaa gcggctagaa
     3841 ttgatggtgc aaataaattt gttacgtttt ataaagttgt gctaccgatt gtagcaccag
     3901 gtattgtagc aacagctatt tatacattta tcaatgcttg gaatgaattc ctgtatgcct
     3961 tgattttgat taacaataca ggaaagatga cagtagcagt agcccttcgt tcacttaatg
     4021 gttcagaaat actagactgg ggagatatga tggcagcgtc tgttattgta gttcttccat
     4081 caattatttt cttctctatc atccaaaata agattgcaag tggattatca gaaggatctg
     4141 tgaagtagac gaaagaagga aaaaaatgaa taaaagaggt ctttattcaa aactaggaat
     4201 ttccgttgta ggcattagtc ttttaatggg agtccccact ttgattcatg cgaatgaatt
     4261 aaactatggt caactgtcca tatctcctat ttttcaagga ggttcatatc aactgaacaa
     4321 taagagtata gatatcagct ctttgttatt agataaattg tctggagaga gtcagacagt
     4381 agtaatgaaa tttaaagcag ataaaccaaa ctctcttcaa gctttgtttg gcctatctaa
     4441 tagtaaagca ggctttaaaa ataattactt ttcaattttc atgagagatt ctggtgagat
     4501 aggtgtagaa ataagagacg cccaaaaggg aataaattat ttattttcca gaccagcttc
     4561 attatgggga aaacataaag gacaggcagt tgaaaataca ctagtatttg tatctgattc
     4621 taaagataaa acatacacaa tgtatgttaa tggaatagaa gtgttctctg aaacagttga
     4681 tacatttttg ccaatttcaa atataaatgg tatagataag gcaacactag gagctgttaa
     4741 tcgtgaaggt aaggaacatt acctcgcaaa aggaagtatt gatgaaatca gtctatttaa
     4801 caaagcaatt agtgatcagg aagtttcaac tattcccttg tcaaatccat ttcagttaat
     4861 tttccaatca ggagattcta ctcaagctaa ctattttaga ataccgacac tatatacatt
     4921 aagtagtgga agagttctat caagtattga tgcacgttat ggtgggactc atgattctaa
     4981 aagtaagatt aatattgcca cttcttatag tgatgataat gggaaaacgt ggagtgagcc
     5041 aatttttgct atgaagttta atgactatga ggagcagtta gtttactggc cacgagataa
     5101 taaattaaag aatagtcaaa ttagtggaag tgcttcattc atagattcat ccattgttga
     5161 agataaaaaa tctgggaaaa cgatattact agctgatgtt atgcctgcgg gtattggaaa
     5221 taataatgca aataaagccg actcaggttt taaagaaata aatggtcatt attatttaaa
     5281 actaaagaag aatggagata acgatttccg ttatacagtt agagaaaatg gtgtcgttta
     5341 taatgaaaca actaataaac ctacaaatta tactataaat gataagtatg aagttttgga
     5401 gggaggaaag tctttaacag tcgaacaata ttcggttgat tttgatagtg gctctttaag
     5461 agaaaggcat aatggaaaac aggttcctat gaatgttttc tacaaagatt cgttatttaa
     5521 agtgactcct actaattata tagcaatgac aactagtcag aatagaggag agagttggga
     5581 acaatttaag ttgttgcctc cgttcttagg agaaaaacat aatggaactt acttatgtcc
     5641 cggacaaggt ttagcattaa aatcaagtaa cagattgatt tttgcaacat atactagtgg
     5701 agaactaacc tatctcattt ctgatgatag tggtcaaaca tggaagaaat cctcagcttc
     5761 aattccgttt aaaaatgcaa cagcagaagc acaaatggtt gaactgagag atggtgtgat
     5821 tagaacattc tttagaacca ctacaggtaa gatagcttat atgactagta gagattctgg
     5881 agaaacatgg tcgaaagttt cgtatattga tggaatccaa caaacttcat atggcacaca
     5941 agtatctgca attaaatact ctcaattaat tgatggaaaa gaagcagtca ttttgagtac
     6001 accaaattct agaagtggcc gcaagggagg ccaattagtt gtcggtttag tcaataaaga
     6061 agatgatagt attgattgga aataccacta tgatattgat ttgccttcgt atggttatgc
     6121 ctattctgcg attacagaat tgccaaatca tcacataggt gtactgtttg aaaaatatga
     6181 ttcgtggtcg agaaatgaat tgcatttaag caatgtagtt cagtatatag atttggaaat
     6241 taatgattta acaaaataaa ggagaaaaac atggttaaat acggtgttgt tggaacaggg
     6301 tattttggag ctgaattggc tcgctacatg caaaagaatg atggagcaga gattactctt
     6361 ctctatgatc cagataatgc agaggcgatt gcagaagaat tgggagcaaa agtagcaagt
     6421 tccttagatg agttggtttc tagcgatgaa gtagattgtg ttatcgtcgc aactccaaat
     6481 aatcttcata aggaaccggt tattaaggct gcacagcatg gtaaaaatgt tttctgtgaa
     6541 aaaccaattg cgctttctta tcaagattgt cgcgagatgg tagatgcgtg taaagaaaac
     6601 aatgtaacct ttatggcagg acatattatg aatttcttta atggtgttca tcatgcaaaa
     6661 gaactcatta atcaaggagt tatcggagac gttctatatt gtcatacagc tcgtaatggt
     6721 tgggaagaac aacaaccgtc agtatcatgg aaaaaaattc gtgaaaaatc aggtggtcac
     6781 ttgtatcacc acatccatga attggattgc gttcaattcc ttatgggggg catgcctgaa
     6841 actgtaacca tgacaggtgg aaatgtggcc catgaaggtg aacatttcgg tgatgaagat
     6901 gatatgattt ttgtcaatat ggaattttct aataagcgtt ttgccttgtt agaatggggt
     6961 tcagcttatc gttggggtga acattatgtc ttaatccaag gaagcaaagg tgccatccgc
     7021 ttagacttat tcaactgtaa aggaactctt aagctagatg ggcaagaaag ctatttcttg
     7081 attcacgaat cgcaagaaga agatgatgat cggactcgta tctatcatag tacagagatg
     7141 gatggagcaa ttgcttatgg taaaccaggt aaacgtactc cattatggct atcatctgtc
     7201 attgataaag aaatgcgcta tctgcatgag attatggaag gagctccagt atcagaagaa
     7261 tttgcaaaac ttttgacagg tgaagctgcc ctagaagcaa ttgctactgc agatgcttgt
     7321 acccagtcta tgtttgaaga tcgcaaagta aaattgtcag aaattgtaaa ataaattttg
     7381 gtattctcct atttataggt cgacttgctc ctctgaaagt acttttagag gagctgtttg
     7441 actttgctag tttttgaaac tgaaatctat tatactacaa actattgaaa gcgttttaat
     7501 tttaaggtat aataatctca tagaaataaa gaaaaggagg aaagaggatg ccacagatta
     7561 gcaaagaagc cttgattgag caaatcaaag atggaatcat cgtttcttgt caggctcttc
     7621 ctcatgaacc gctttataca gaagcgggag gggtgattcc cttgctggtc aaagcggctg
     7681 agcaaggtgg agcagtcggt atccgagcaa acagtgttcg cgatatcaag gaaattaagg
     7741 aagtcactaa acttccaatc attgggatta tcaaacgtga ttatccacct caggaaccct
     7801 tcatcacggc tactatgaaa gaagttgatg aattggcaga actggacatc gaggtgattg
     7861 ctctggattg taccaagcgt gaacgctacg atggtttgga aattcaagag ttcattcgtc
     7921 aggttaagga gaaatatcct aatcagcttt tgatggctga tactagtatc ttcgaagaag
     7981 ggctagcagc tgtagaagca ggaattgact ttgtcggaac aaccttatca ggctacacat
     8041 cctacagtcc aaaagtagac ggtccagatt ttgaattgat taagaaactc tgtgatgctg
     8101 gtgtagatgt cattgcagaa ggaaaaattc atacaccaga acaagccaaa caaatccttg
     8161 aatatggagt gcgaggcatc gttgttggtg gcgccattac tagaccaaaa gagattacag
     8221 aacgcttcgt tgctagtctt aaataagatg tgagggggag ttttatgttt aaagttttac
     8281 aaaaagttgg aaaagctttt atgttaccta tagctatact tcctgcagca ggtctacttt
     8341 tggggattgg tggtgcactt tcaaacccaa ccacgatagc aacttatcca atactagaca
     8401 atagtatttt tcaatcaata ttccaagtaa tgagctctgc aggagaggtt gtattcagta
     8461 atttgtcact acttctctgt gtgggattat gtattggctt agcgaaacga gataaaggaa
     8521 ccgctgcgtt agcaggagta actggttact tagttatgac tgcaacgatc aaagctttgg
     8581 taaaactttt tatggcagaa ggatctgcaa ttgatactgg agttattgga gcattagttg
     8641 tcggaatagt tgccgtatat ttgcacaacc gatataacaa tattcaatta ccttccgctt
     8701 taggattctt tggaggttca cgcttcgttc ctattgttac atcgttctct tctatcttga
     8761 ttggctttgt cttctttgtt atttggccac ctttccaaca acttcttgtt tctacaggtg
     8821 gatatatttc tcaggcgggt ccaattggaa cttttctata tggattttta atgagacttt
     8881 ctggagcagt aggcttacat catataattt accctatgtt ttggtatact gaacttggtg
     8941 gtgttgaaac tgttgcagga caaacagtgg ttggagctca aaaaatattt tttgctcaat
     9001 tagccgattt ggcccattct ggattattta cagaaggaac aaggtttttt gcaggtcgtt
     9061 tctcaacaat gatgttcggt ttaccggctg cctgtttagc gatgtaccat agtgttccta
     9121 aaaatcgtcg taaaaaatac gcgggtttgt tttttggagt tgctttaaca tcttttatta
     9181 ccggtattac agaaccaatt gaatttatgt ttctattcgt cagtccggtt ctatatgttg
     9241 ttcacgcatt ccttgatggt gttagcttct ttattgcaga cgtcttaaat atttcaatag
     9301 gaaacacatt ttcaggaggt gtaatcgatt tcactttatt tggaattttg caggggaacg
     9361 ctaagacgaa ttgggttctt cagattccat ttggacttat ttggagtgtt ttgtattata
     9421 ttatttttag atggttcatt actcaattca acgttctaac gccagggcga ggagaagaag
     9481 tagattctaa agaaatttct gaatccgcag attcaacttc aaatactgca gattatttaa
     9541 aacaggatag cctacaaatt atcagagcct tgggtggatc aaataatata gaagatgtag
     9601 atgcttgtgt gacacgttta cgtgtagctg taaaagaagt taatcaagtt gataaagcac
     9661 ttttaaaaca aattggtgca gttgatgtct tagaagtgaa gggtggcatt caagcaatct
     9721 atggagcaaa agcaatctta tataaaaata gtattaatga aattttaggt gtagatgatt
     9781 aagtacttac tgacttaata aaaaacagag gagagtgatg gatgagtagg atgaaatgaa
     9841 atcgcataca agaaataaag aactcattat ccaagttgga tacgcttatt acataggaga
     9901 atacaaatga aatttagaaa attagcttgt acagtacttg cgggtgctgc ggttcttggt
     9961 cttgctgctt gtggcaattc tggcggaagt aaagatgctg ccaaatcagg tggtgacggt
    10021 gccaaaacag aaatcacttg gtgggcattc ccagtattta cccaagaaaa aactggtgac
    10081 ggtgttggaa cttatgaaaa atcaatcatc gaagcgtttg aaaaagcaaa cccagatata
    10141 aaagtgaaat tggaaaccat cgacttcaag tcaggtcctg aaaaaatcac aacagccatc
    10201 gaagcaggaa cagctccaga cgtactcttt gatgcaccag gacgtatcat ccaatacggt
    10261 aaaaacggta aattggctga gttgaatgac ctcttcacag atgaatttgt taaagatgtc
    10321 aacaatgaaa acatcgtaca agcaagtaaa gctggagaca aggcttatat gtatccgatt
    10381 agttctgccc cattctacat ggcaatgaac aagaaaatgt tagaagatgc tggagtagca
    10441 aaccttgtaa aagaaggttg gacaactgat gattttgaaa aagtattgaa agcacttaaa
    10501 gacaagggtt acacaccagg ttcattgttc agttctggtc aagggggaga ccaaggaaca
    10561 cgtgccttta tctctaacct ttatagcggt tctgtaacag atgaaaaagt tagcaaatat
    10621 acaactgatg atcctaaatt cgtcaaaggt cttgaaaaag caactagctg gattaaagac
    10681 aatttgatca ataatggttc acaatttgac ggtggggcag atatccaaaa ctttgccaac
    10741 ggtcaaacat cttacacaat cctttgggca ccagctcaaa atggtatcca agctaaactt
    10801 ttagaagcaa gtaaggtaga agtggtagaa gtaccattcc catcagacga aggtaagcca
    10861 gctcttgagt accttgtaaa cgggtttgca gtattcaaca ataaagacga caagaaagtc
    10921 gctgcatcta agaaattcat ccagtttatc gcagatgaca aggagtgggg acctaaagac
    10981 gtagttcgta caggtgcttt cccagtccgt acttcatttg gaaaacttta tgaagacaaa
    11041 cgcatggaaa caatcagcgg ctggactcaa tactactcac catactacaa cactattgat
    11101 ggatttgctg aaatgagaac actttggttc ccaatgttgc aatctgtatc aaatggtgac
    11161 gaaaaaccag cagatgcttt gaaagccttc actgaaaaag cgaacgaaac aatcaaaaaa
    11221 gctatgaaac aatagtcctt agttattcta taaaaagtag ttttttaaag aacctaagag
    11281 tgtatacccc cttttccctc tacacagata gtgtaagaaa agggggcttt tgtttaaaat
    11341 gtaagaaact gtcacgaaat taaaatgaag ttcttacata agcgaatcat aaaaaatttc
    11401 attttgattt taaaacagtt caagaaagtc aaaaaattat tctatttgaa agagaggtgc
    11461 cgactgtgaa agtcaataaa atccgtatgc gggaaacagt gatttcctac gctttcctag
    11521 caccagtatt attcttcttt gtcatctttg tgttggctcc gatggtgatg ggcttcatta
    11581 caagtttctt taactactca atgactaaat ttgagtttgt aggcttggat aactatatcc
    11641 gtatgtttaa agatcctgtc tttacaaaat ctctgattaa cacagttatt ttggttattg
    11701 gatctgtacc agttgttgtt ctattctcac tctttgtagc atctcagacc tatcatcaaa
    11761 atgtcattgc cagatccttc taccgtttcg tcttcttcct tcctgttgta acgggtagtg
    11821 ttgccgtgac agttgtttgg aaatggattt atgacccact atcagggatt ctaaactttg
    11881 tccttaagtc cagccacatc atcagccaaa acatttcttg gttgggagat aaaaactggg
    11941 cattgatggc gattatgatt attctcttga ccacttcagt tggtcagccc atcatccttt
    12001 atatcgctgc catggggaat attgacaatt cactggttga agcggcgcgt gttgatggtg
    12061 caactgagtt tcaagttttt tggaagatta aatggccaag ccttcttcca acaactcttt
    12121 atattgcaat catcacaaca attaactcat tccagtgttt cgccttgatt cagcttttga
    12181 catctggtgg tccaaactac tcaacaagta ccttgatgta ctacctttac gaaaaagcct
    12241 tccaattgac agaatacggc tatgccaaca caattggtgt cttcttggca gtcatgattg
    12301 ctatcgtaag ctttgttcaa tttaaagtac ttggaaacga cgtagaatac taaagaaagg
    12361 agacagctat gcaatctaca gaaaaaaaac cattaacagc ctttactgtt atttcaacaa
    12421 tcattttgct cttgttgact gtgctgttca tctttccatt ctactggatt ttgacagggg
    12481 cattcaaatc acaacctgat acaattgtta ttcctcctca gtggttccct aaaatgccaa
    12541 ccatggaaaa cttccaacaa ctcatggtgc agaaccctgc cttgcaatgg atgtggaact
    12601 cagtatttat ctcattggta accatgttct tagtttgtgc aacctcatct ctagcaggtt
    12661 atgtattggc taaaaaacgt ttctatggtc aacgcattct atttgctatc tttatcgctg
    12721 ctatggcgct tccaaaacaa gttgtccttg taccattggt acgtatcgtc aacttcatgg
    12781 gaatccatga tactctctgg gcagttatct tgcctttgat tggatggcca ttcggtgtct
    12841 tcctcatgaa acagttcagt gaaaatatcc ctacagagtt gcttgaatca gctaaaatcg
    12901 acggttgtgg tgagattcgt accttctgga gtgtagcctt cccgattgtg aaaccagggt
    12961 ttgcagccct tgcaatcttt accttcatca atacttggaa tgactacttc atgcaattgg
    13021 taatgttgac ttcacgtaac aatttgacca tctcacttgg ggttgcgacc atgcaggctg
    13081 aaatggcaac caactatggt ttgattatgg caggagctgc ccttgctgct gttccaatcg
    13141 tcacagtctt cctagtcttc caaaaatcct tcacacaggg tattactatg ggagcggtca
    13201 aaggataata ctctgcgaaa atctcttcaa actacgtcag cttcaccttg ccatacttaa
    13261 gtattgcctg cggttagctt cctagtttgt tcttcaattt tcattgagta taggaaaatc
    13321 aatctatcaa gatacagaag tatattttat agatttagag aatatagagg ttataagtgt
    13381 ctacaaaatg gagggtatgc agttacttta tgaagttttg tcagacactt ataaacttaa
    13441 gaatggtttt agttaactat cagaaacgaa ggaaagagta tgatttttga cgatttgaaa
    13501 aacatcacct tttacaaagg gattcatcct aatttagaca aggctatcga ctatctctac
    13561 caacatcgta aggattcttt cgaattagga aagtatgata ttgatggaga taaagtcttt
    13621 ctagttgttc aggaaaatgt cctcaatcaa gctgaaaatg atcaatttga gtatcataag
    13681 aactatgcag atttgcattt gctggtagaa ggacatgaat attcgagcta cggttcacgt
    13741 atcaaagacg aggcagtagc attcgacgaa gcgagtgaca ttggctttgt tcattgtcat
    13801 gaacactacc cactcttgtt gggttatcac aattttgcga ttttcttccc aggtgagcca
    13861 catcagccaa atggttatgc aggcatggaa gaaaaggttc gaaaatatct ctttaaaatt
    13921 ttgattgatt aaaaatagga tgaattgttt ttttgtaaag ctttgataat actctaccat
    13981 gaaattgatc tttgtgaggt agagaaatga gaataaaata tttaaaaatt ggtatcttct
    14041 aagtatgctg caagagctag tttcttagat ggacagggga ttacagttga tgagatggct
    14101 tggataatta ggggcattgt gaatgcattg attggtagat acataaaatt aggtacttat
    14161 gcggctaagt atggtattag tatggcacgc tcgatcttaa gtagggtagc tgcaactgca
    14221 gcagcaagag taggattact gaccaagatt tctggatgga ttttacgagt agctgtgaat
    14281 gtagctgatg tatatggtaa ttttgccaac aatattgctg cagcttggga tgcatatgat
    14341 aaaattccta acaatggtcg tataaacttt taaaatgcga gaatgaaagc actttgtatt
    14401 tttttattga atatgttagc ttggacagtg cttgcaatga taattcgtgg agggctagat
    14461 ggatttgata ggcatacttg gagtactatt ttaattgctt cgctgttcgg ggtatatgat
    14521 tataagccca tagataaaaa tagaaaaaag tccaaaagaa aaaatagatt tgttcatggt
    14581 agggacttat gaaagcttta ctgacaaaaa agaaaacagt ttacaaagaa aaatgatgga
    14641 ggagcaaaca tggcacaaaa aggagtaagc cttatcaagg cagcatttga tacagataac
    14701 tttctcatgc gttttagtga gaaggtcttg gacatcgtga cagccaatct tctttttgtc
    14761 gtctcttgtt tacccatcgt gacgattgga gtggctaaaa tcagcctcta cgagaccatg
    14821 ttcgaagtta agaagagcag acgggtgcct gtttttaaaa tctatctaag atctttcaag
    14881 caaaatctga aactaggtct tcagctgggt ttaatggagt taggaattgt gtttcttacc
    14941 ctttcagatc tctatctttt ctggggtcaa acagctctgc ccttccaatt gctgaaagcc
    15001 atttgtttag gtattctgat ttttcttact atcgtgatgc tggctagtta ccctatcgcg
    15061 gcacgttatg acctatcttg gaaagaaatt cttcaaaaag gattgatgtt ggctagtttt
    15121 aactttcctt ggttcttcct catgttagcc attcttgtcc tcattgtgat ggttctttat
    15181 ctgtccgcct tcagtctact cttaggtggc tcagtcttcc tactttttgg gtttggacta
    15241 ttggtcttta tccagactgg attgatggag aaaattttcg caaaatacca ataggagctt
    15301 tatttctgaa actactttca aaggctccaa acgctattct ataagcgaga aactaaaatc
    15361 taagtgtcta taagatattg aaagcctttt cggtaaagtg tatactaaag aagtaaagaa
    15421 aaatctgctt tagcagaaaa taaaaataat aggagaaaat ctatgtcaga tttgaaaaaa
    15481 tacgaaggtg tcattccagc cttctacgca tgttatgatg atcaaggaga agtaagccca
    15541 gaacgtacgc gtgccttggt tcaatacttc attgataaag gtgttcaagg tctttatgtc
    15601 aatggttctt ctggtgaatg tatctaccaa agcgttgaag atcgcaagtt gattttggaa
    15661 gaagtcatgg cggtagccaa aggtaaattg accattattg cccatgttgc ttgcaataat
    15721 actaaagata gtatggaact tgctcgccat gctgaaagct tgggagtaga tgctattgca
    15781 acaattccac caatttattt ccgcttgcca gaatactcag ttgccaaata ctggaacgat
    15841 atcagttctg cagctccaaa cacagactac gtgatttaca acattcctca attggcaggg
    15901 gttgctttga ctccaagcct ttacacagaa atgttgaaaa atcctcgtgt tatcggtgtg
    15961 aagaactctt ctatgccagt tcaagatatc caaacctttg tcagccttgg tggagaagac
    16021 catatcgtct ttaatggtcc tgatgagcag ttcctaggag gacgcctcat gggggctagg
    16081 gctggtatcg gtggtactta tggtgctatg ccagaactct tcttgaaact caatcagttg
    16141 attgcggata aggacctaga aacagcgcgt gaattgcagt atgctatcaa cgcaatcatt
    16201 ggtaaactca cttctgctca tggaaatatg tacggtgtca tcaaagaagt cttgaaaatc
    16261 aatgaaggct tgaatattgg atctgttcgt tcaccattga caccagtgac tgaagaagat
    16321 cgtccagttg tagaagcggc tgctgccttg attcgtgaaa ccaaggagcg cttcctctaa
    16381 tctaaaagga ggtatttatg acatattacg ttgcaattga tatcggtgga accaacatca
    16441 agtatggttt ggttgatcaa gaggggcaac ttcttgaatc gcatgaaatg ccaactgagg
    16501 cgcataaggg tggacctcat atcttacaaa agaccaaaga tatcgtagct agttatttag
    16561 aaaaaggccc agtagcaggt gttgccatat cttctgctgg gatggtggat ccggataagg
    16621 gtgagatttt ctatgctggg ccgcaaatcc ctaactacgc aggcacccag ttcaaaaagg
    16681 aaatcgaaga aagctttact attccttgtg agattgaaaa tgatgtcaac tgtgcaggtc
    16741 ttgctgaggc agtatctggt tcaggcaagg gagcaagtgt gacactttgc ttgaccattg
    16801 gaaccggtat cggtggttgc ttgattatgg ataggaaagt cttccatggt tttagcaatt
    16861 cagcctgtga agtcgggtat atgcatatgc aggatggagc ttttcaagac ttggcttcta
    16921 caacagcttt agtgaaatat gtagctgaag cccatggaga agatgttgat cagtggaatg
    16981 gccgtagaat tttcaaagaa gccactgaag gaaacaaaat ctgcatggaa ggtattgacc
    17041 gtatggttga ctatctagga aaaggtctgg caaatatttg ctacgttgcc aatccagaag
    17101 tggttattct tggtggtggt atcatggggc aagaggctat cctcaaacct aagatccgta
    17161 cagccttgaa agaggctttg gtaccaagtt tagcagaaaa aacacgatta gaatttgccc
    17221 atcaccaaaa tacagcaggg atgttgggtg catattatca ttttaagaca aaacaatcct
    17281 agtttggctc agccaaacta ggattttctt acacgttttt gtctacgata gccgttgagt
    17341 tttttatttt cccagtagct attaaagatt ttttccttgc tttcgcgatt gatttccaaa
    17401 aagtaggcat aaatcaaatc gataaagaag agcataggaa gttgagcgga tattcgttgg
    17461 atataggagg gttggctgtg ggtggctaca agaacagtct ctgtataggt ctggctatct
    17521 ttattgggaa cacttgtaaa gagtacagtc tttgccccca tctccttagc atctaataga
    17581 ctatctaaaa tagaaggagt tgagcctgaa agtgagaagc ccagtactag acaattttca
    17641 tccatgatgc tggttgtcca ggcaaagccg tcttggtctg tcaaagcttc gcagaccaca
    17701 cctagtcgca taaaacgtaa tttcatttca cgggcgacga ggccagaact ccctgttcca
    17761 aagaagtaga tacgctcagc atcttcgatt agctgggcaa ttcgttctag ttggatttcg
    17821 tcaatcaagt cttgtgtttg ttccctcata ttgctataac ttctgaggac tcgtttggtc
    17881 agtggactgt gcttggagac ttggttggct tgattttctg cctgatgttg gtattggaaa
    17941 ataaattctc ggtagccagt aaagccacac tttttagcaa agcgggtcaa agcagcttga
    18001 gaaatatgta atttttgggt gacttgttga gaagataaat catctgtaat cgtttcagct
    18061 tgcaaaaaat agcgagcgat ttcttgttct aggtctgtca tttcttcaaa atgtgaatca
    18121 atgatagttg cgatatctgg tttgtccata gggaaagctc ctttacatga gtcatactgg
    18181 aagactagat cagagaatag tcacacttca ttataacaca taatataagg atagataaat
    18241 aaaaacgcat ctctgtttta aaaacgaaaa aatcgaaaaa gcttctctct tttccataat
    18301 tttctactca aattgtggta caattaagag taagatttta agttagaaat gagactgatt
    18361 tgtatgagaa aatttaacag ccattcgatt ccgattcggc ttaatttatt gttttcaatc
    18421 gtcattttac tctttatgac cattattggt cgtttgttgt atatgcaggt tttgaacaag
    18481 gatttttacg aaaaaaagct agcttcagct agtcagacca agattacaag cagttcagcc
    18541 cgtggggaaa tttatgatgc tagtggaaaa cctttggtag aaaatacgtt aaagcaggtt
    18601 gtttccttta cgcgtagcaa taaaatgacg gctacagact taaaagaaac agctaaaaag
    18661 ttactgactt atgtgagcat cagttctcca aatttgacag aacgccagct ggcggattac
    18721 tatttggctg atcctgaaat ctataaaaaa atagtggaag ctctcccaag tgagaaacgc
    18781 ttggattcag atggcaatcg tctatccgaa tcagaactgt ataacaatgc ggtcgatagt
    18841 gtacaaacga gtcaactaaa ctatacagag gatgaaaaga aagaaatcta tctttttagt
    18901 cagttaaatg ctgttggaaa ctttgcgaca ggaaccattg cgacagatcc tctaaatgat
    18961 tctcaggtgg ctgttattgc ctctatttca aaggagatgc ctggcattag tatttctact
    19021 tcttgggata gaaaggtttt ggaaacttcc ctttcttcta tagttgggag tgtatccagt
    19081 gaaaaagctg gtctcccagc ggaagaagca gaagcctatc ttaaaaaagg ctattctcta
    19141 aatgaccgtg taggaacctc ctatttggaa aagcaatatg aagagacctt acaaggaaaa
    19201 cgctcggtaa aagaaatcca tctggataaa tatggcaata tggaaagcgt ggatacaatt
    19261 gaggaaggta gtaagggaaa caatatcaaa ctgaccattg atttggcttt ccaagatagc
    19321 gtggatgctt tactgaaaag ttatttcaat tctgagctag aaaatggtgg agccaagtat
    19381 tctgaaggtg tctatgcagt cgcccttaac ccaaaaacag gtgcggtttt gtctatgtca
    19441 gggattaaac atgacttgaa aacgggagag ttgacgcctg attccttggg aacggtaacc
    19501 aatgtctttg ttccaggttc ggttgtcaag gcggcgacca tcagctcagg ttgggaaaat
    19561 ggagtcttgt caggaaacca gaccttgaca gaccagtcca ttgtcttcca aggttcagct
    19621 cccatcaatt cttggtatac tcaggcttac ggttcattcc ctatcacagc ggtccaagct
    19681 ctggagtatt catcaaatac ctatatggtc caaacagcct taggtcttat ggggcaaacc
    19741 tatcaaccca atatgtttgt cggcaccagc aatctagagt ctgctatgga gaaactgcgt
    19801 tcaacctttg gcgaatatgg cttgggtact gcgacaggaa ttgacctacc agatgaatct
    19861 actggatttg ttcccaaaga gtatagcttt gctaattaca ttactaatgc ctttgggcag
    19921 tttgataact atacgccgat gcagttggct cagtatgtag caactattgc aaataatggt
    19981 gttcgtgtgg ctcctcgtat tgttgaaggc atttatggta ataatgataa gggaggactg
    20041 ggtgacttga ttcagcaact gcaaccgaca gagatgaata aggtcaatat atccgactcc
    20101 gatatgagca tcttgcacca aggtttttat caggttgccc atggtactag tggattgaca
    20161 actggacgtg ccttttcaaa tggtgccttg gtatccatta gcggaaaaac aggtacagcc
    20221 gaaagctatg tggcagatgg tcagcaagca accaatacca atgcggtggc ctatgcccca
    20281 tctgataatc cccaaatcgc tgtcgcagtg gtctttcctc ataataccaa tctaacaaat
    20341 ggtgtaggac cttccattgc gcgtgacatt atcaatctgt atcaaaaata ccatccaatg
    20401 aattagaaag gaaattatgc tttatccaac acctattgcc aagttgattg acagttattc
    20461 taagttacca ggtatcggga ttaagacggc tacgcgtctg gccttttata cgattgggat
    20521 gtctgctgat gatgtcaatg aatttgcaaa aaatctcctt tctgctaaga gagaattgac
    20581 atattgttct atttgtggac gtttgacaga cgacgatcct tgttctatct gtactgatcc
    20641 gactcgtgac cagacaacaa ttttagttct tgaggatagt agagatgtgg cagccatgga
    20701 aaatatccaa gaataccatg gactctatca tgtccttcat ggcctcattt ctcctatgaa
    20761 tggtatcagt ccggacgata tcaatctcaa gagccttatg actcgtctta tggatagtga
    20821 ggtttcagaa gtgattgtgg cgactaatgc tacagcggat ggtgaagcga cttccatgta
    20881 tctttcacgt ttgctcaagc cggctggtat caaggttacg cgtctagcac gaggtctcgc
    20941 tgtgggagcg gacattgagt atgcggacga agtgacactc ttacgagcca ttgaaaatcg
    21001 gacagagttg taagtgtagg caaatttacg aactccattc atttataaaa aatcaaagag
    21061 gctgaaaatc gttcctatcg gcctcttttt gtatagtgtg atgagtaggc tcaggttcaa
    21121 gttttaaaaa accaagcaaa tatgatatac taaagagcga gtattctagt agaattagga
    21181 caaataatat gaaacaaacg attattcttt tatatggtgg acggagtgcg gaacgcgaag
    21241 tctctgtcct ttcagctgag agtgtcatgc gtgcggtcga ttacgaccgt ttcacagtca
    21301 agactttctt tatcagtcag tcaggtgact ttatcaaaac acaggaattt agtcatgctc
    21361 cggggcaaga agaccgtctc atgaccaatg aaaccattga ttgggataag aaagttgcac
    21421 caagtgctat ctacgaagaa ggtgcagtgg tctttccagt ccttcacggg ccaatgggag
    21481 aagatggctc tgttcaagga ttcttggaag ttttgaaaat gccttacgtt ggttgcaaca
    21541 ttttgtcatc aagtcttgcc atggataaaa tcacgactaa gcgtgttctg gaatctgctg
    21601 gtattgccca agttccttat gtggctatcg ttgaaggcga tgatgtgact gctaaaatcg
    21661 ctgaagtgga agaaaaattg gcttatccag tcttcactaa gccgtcaaac atggggtcta
    21721 gtgtcggtat ttctaagtct gaaaaccaag aagaactccg tcaagcctta aaacttgcct
    21781 tccgatatga cagccgtgtc ttggttgagc aaggagtgaa tgcccgtgaa attgaggttg
    21841 gcctcttggg taactacgat gtcaagagca cgctaccagg agaagttgtc aaggacgttg
    21901 ccttttatga ctacgatgcc aagtatattg ataacaatat tactatggat attcctgcca
    21961 aaatcagtga tgatgtggtg gctgtcatgc gtcaaaatgc agaaacagcc ttccgtgcca
    22021 ttggtggcct tggtctatct cgttgcgatt tcttctatac agataaggga gagatttttc
    22081 tcaacgagct caatactatg ccaggtttca cccagtggtc tatgtaccca ctactttggg
    22141 acaatatggg gatcagctac ccaaaactaa tcgagcgttt ggttgacctt gccaaggaaa
    22201 gttttgacaa gcgcgaagcg catttgatat aaaatgaaag agagggtaga agccagaacc
    22261 atcactgcac ggtgactaga gttctcggac ttcagccctt tttaaaggag tagaaatgaa
    22321 attaacaatc catgaaattg cccaagttgt tggagccaaa aatgatatca gtatctttga
    22381 ggacacccag ttagaaaaag ctgagtttga tagtcgtttg attggaactg gagatttatt
    22441 tgtgccactt aaaggtgcgc gtgatggcca tgactttatt gaaacagcct ttgaaaatgg
    22501 tgcagcagta accttgtctg agaaagaggt ctcaaatcat ccttacattc tagtagatga
    22561 tgttttgaca gcctttcaat ccttagcatc ctactatctt gaaaaaacga ctgttgatgt
    22621 ctttgctgtt acaggttcaa atggcaagac aacgactaag gatatgttgg cgcatttact
    22681 gtcaacaaga tacaagacct acaaaacaca aggcaattac aataatgaga ttggccttcc
    22741 ttacacagtt cttcatatgc ctgaaggaac agaaaagttg gttttggaga tgggacagga
    22801 tcacttgggc gatattcatc tcttgtctga attggctcgt ccaaaaacag ccatcgtgac
    22861 cttggttgga gaagcccatt tggccttttt caaagaccgt tcagagattg ctaagggaaa
    22921 aatgcaaatt gcagacggaa tggcttcagg ttccttgctt ttagcgccgg ctgaccctat
    22981 cgtagaggac tatttgccaa ctgataaaaa ggtggttcgt tttgggcaag gggcagagct
    23041 ggaaattact gacttggttg agcgcaaaga tagtctgacc ttcaaggcca atttcttaga
    23101 gcaagccctt gatttgccag taactggcaa gtacaatgcg acaaatgcta tgattgcatc
    23161 ctatgttgcc ttgcaagaag gagtttcaga ggagcaaatt cgtttggcct tccaagatct
    23221 tgaattgacg cgtaaccgta ccgagtggaa gaaagcagcc aatggagcag atatcctgtc
    23281 agatgtttac aatgccaatc caactgctat gaaactgatt ttagagactt tctctgccat
    23341 tccagccaat gaaggtggca agaaaattgc agtgttggcg gatatgaagg agcttggtga
    23401 ccagtctgtt caacttcata atcagatgat tttgagcctt tctccagatg tgcttgatac
    23461 cgtgattttc tatggagaaa atattgctga attagcccaa ttggccagtc aaatgttccc
    23521 aatcggccac gtttactact tcaagaaaac agaagaccag gatcaatttg aagacctagt
    23581 caagcaggtc aaggaaagcc ttggagccca tgaccaaatc ctgctcaaag gctctaactc
    23641 tatgaatcta gccaagttgg tagaaagttt agaaaatgaa gacaagtgat tttgtcaagt
    23701 atttgcaaag aatgattgcc attacagata ctggcttaac ctttacaaaa gatccgtttg
    23761 accgtgagcg ctacgaagac ttgcgaagtc tgttatctga aatgttgaat caagcatcag
    23821 accttgattc cgaagaagtg gcagaagtct tgaagccaac ttctgcttat gcgactccgt
    23881 taatggacgt ccgtgcttgg attgttgagg atgagaagat ttgtctggtt aggggacaag
    23941 gagaggatag ttgggctttg ccaggtggct ttggtgaagt cggctattct ccaacagaaa
    24001 atattctcaa ggaaattgaa gaagaaaccg gttttaaagc caaagttgaa agactgctag
    24061 ctgtttttga taccaatcgt ttccaactac agagcaaaca gtatacaaag tttgtttttg
    24121 gatgtaagct tcttgatgga caattccaag aaaatcaaga aattgctgac cttcaatttt
    24181 ttgccattga ccaactgccg aacttatctg aaaaacgcat taccaaggag caaatagagc
    24241 ttctttggca ggtttatcaa ggtcataggg ggcaatatct tgactaagaa gatgattatc
    24301 gtatttctaa atccattttt aacaactagc atggtataat aatatgcagg aaaattttga
    24361 attatgagga agactagatg aatttatggg atattttctt tacgactcag gcaaccgagc
    24421 cgcccaaatt tgaccttttt tggtatgtta gcctatttac gctcttagcc ttaacctttt
    24481 atacagccca tcgctatcgt gaaaagaagg tttaccaacg atttttccaa atcttgcaga
    24541 ctgttcagtt aatccttctt tatggttggt actgggtcaa tcatatgcca ctgtcagaaa
    24601 gcctaccctt ttaccattgc cgtatggcta tgtttgtggt actcttgctt cctggtcaat
    24661 ccaaatataa acaatacttt gcattattgg gaacatttgg gacattagca gcctttgttt
    24721 atccagtgcc agatgcttac ccttttccac atatcaccat tctatccttt atctttggtc
    24781 atttagcact cttggggaac tctctagttt atctattgag acagtataat gcgcgattgc
    24841 tggatgtgaa gggaattttt ctcatgacct ttgccctaaa tgccttgatt tttgtggtca
    24901 atttggtgac aggtggcgat tacggatttt tgacaaaacc gccattggtt ggggatcacg
    24961 gtctagtagc taattattta cttgtttcaa ttgtgctggt agctactatc agtttgacta
    25021 agaaaatctt agaattcttt ttagctcaag aagcagaaaa aatgattgca aaggaagctt
    25081 aacacagagc tttctttttt gctcttagag agtttttaca agcagcttat aaaataagaa
    25141 tttctgaata gacaaactca aaaaatggct gggaaattta ggaaaaaagc aagcacgatt
    25201 aaattttttg tgttataata ttttgtgaat agctatgcct atgtttagct atggaataat
    25261 acgaagtgcg aaacttggaa gatagagagg aagcgatgta atggctagag aaggcttttt
    25321 tacaggtcta gatattggaa caagctctgt caaggtgctt gtggccgagc agagaaatgg
    25381 tgaattaaat gtaattggcg tgagtaatgc caaaagtaaa ggtgtaaagg atggaattat
    25441 tgttgatatt gatgcagcag caactgctat caagtcagcc atttcccaag cggaagaaaa
    25501 ggcaggcatt tcgattaaat cagtgaatgt cggcttgcct ggtaatcttt tgcaggtaga
    25561 accaactcag gggatgattc cagtaacatc tgatactaag gaaattacgg atcaagatgt
    25621 tgaaaatgtt gtcaaatcag ctttgacaaa gagtatgaca cctgaccgtg aagtcattac
    25681 ctttattcct gaagaattta ttgtggatgg tttccaaggg attcgtgacc cacgtggcat
    25741 gatgggggtt cgccttgaaa tgcgtggttt gctttataca ggacctcgta ctatcttgca
    25801 caatttgcgt aagacggttg agcgtgcagg tgttcaggtt gaaaatgtta tcatttcacc
    25861 actagcaatg gttcagtctg ttttgaacga aggggaacgt gaatttggtg ctacagtgat
    25921 tgatatgggg gcaggtcaaa cgactgtcgc tacaatccgt aatcaagaac tccagttcac
    25981 acatattctc caagaaggtg gagattatgt aactaaagat atctccaagg ttttgaaaac
    26041 ctctcgcaaa ttagcggaag gcttgaaact gaattacggg gaagcctatc cgcctcttgc
    26101 aagcaaagaa accttccaag tagaggttat tggagaagta gaagcagtcg aagtgacgga
    26161 agcctacttg tcagaaatta tttctgcacg aatcaagcac atccttgaac aaatcaagca
    26221 agaattagat agaaggcgtc tattggacct ccctggtggt attgtcttaa tcggtgggaa
    26281 tgccatttta ccaggtatgg ttgagcttgc tcaggaagtc tttggcgtcc gtgtcaagct
    26341 ttatgttcca aatcaagttg gtatccgtaa tccagccttt gcgcatgtga ttagtttatc
    26401 agaatttgcg ggtcaattaa cagaagttaa tcttttggct cagggagcga taaaaggtga
    26461 gaatgactta agtcatcagc caattagttt tggtgggatg ctgcaaaaaa cagctcagtt
    26521 tgtacaatca acgcctgttc aaccagctcc tgctccagaa gtagagccgg tggcgcctac
    26581 agaaccaatg gcggatttcc aacaagcttc acaaaataaa ccgaaattag cagatcgttt
    26641 ccgtggattg atcggaagca tgtttgacga ataaagagga aaaataaatt atgacatttt
    26701 catttgatac agctgctgct caaggggcag tgattaaagt aattggtgtc ggtggaggtg
    26761 gtggcaatgc catcaaccgt atggtcgacg aaggtgttac aggcgtagaa tttatcgcag
    26821 caaacacaga tgtacaagca ttgagtagta caaaagctga gactgttatt cagttgggac
    26881 ctaaattgac tcgtggtttg ggtgcaggag gtcaacctga ggttggtcgt aaagccgctg
    26941 aagaaagcga agaaacactg acggaagcta ttagtggtgc cgatatggtc ttcatcactg
    27001 ctggtatggg aggaggctct ggaactggag ctgctcctgt tattgctcgt atcgccaaag
    27061 atttaggtgc gcttacagtt ggtgttgtaa cacgtccctt tggttttgaa ggaagtaagc
    27121 gtggacaatt tgctgtagaa ggaatcaatc aacttcgtga gcatgtagac actctattga
    27181 ttatctcaaa caacaatttg cttgaaattg ttgataagaa aacaccgctt ttggaggctc
    27241 ttagcgaagc ggataacgtt cttcgtcaag gtgttcaagg gattaccgat ttgattacca
    27301 atccaggatt gattaacctt gactttgccg atgtgaaaac ggtaatggca aacaaaggga
    27361 atgctcttat gggtattggt atcggtagtg gagaagaacg tgtggtagaa gcggcacgta
    27421 aggcaatcta ttcaccactt cttgaaacaa ctattgacgg tgctgaggat gttatcgtca
    27481 acgttactgg tggtcttgac ttaaccttga ttgaggcaga agaggcttca caaattgtga
    27541 accaggcagc aggtcaagga gtgaacatct ggctcggtac ttcaattgat gaaagtatgc
    27601 gtgatgaaat tcgtgtaaca gttgttgcaa cgggtgttcg tcaagaccgc gtagaaaagg
    27661 ttgtggctcc acaagctaga tctgctacta actaccgtga gacagtgaaa ccagctcatt
    27721 cacatggctt tgatcgtcat tttgatatgg cagaaacagt tgaattgcca aaacaaaatc
    27781 cacgtcgttt ggaaccaact caggcatctg cttttggtga ttgggatctt cgccgtgaat
    27841 cgattgttcg tacaacagat tcagtcgttt ctccagtcga gcgctttgaa gccccaattt
    27901 cacaagatga agatgaattg gatacacctc catttttcaa aaatcgttaa gtaaatgaat
    27961 gtaaaagaaa atacagaact tgtttttcga gaagttgcag aggctagtct gagtgctcat
    28021 cgagagagtg gttcggtctc tgtcattgca gttaccaagt atgtagatgt accgacagcg
    28081 gaagccttgc ttccgctagg tgtccatcat atcggtgaaa atcgtgtaga taagtttctg
    28141 gaaaaatatg aagctttaaa agatcgagat gtgacttggc atttgattgg taccttgcaa
    28201 agacgtaagg tgaaagatgt cattcaatac gttgattatt tccatgcatt ggactcagta
    28261 aagctagcag gggaaattca aaaaagaagt gaccgagtca tcaagtgttt ccttcaagta
    28321 aatatttcta aagaagaaag caaacacggt ttttcgagag aggaactgct ggaaatcttg
    28381 ccagagttag ccagactaga taagattgaa tatgttggtt taatgacgat ggcacctttt
    28441 gaggctagca gtgagcagtt gaaagagatt ttcaaggcgg cccaagattt acaaagagaa
    28501 attcaagaga aacaaattcc aaatatgcct atgaccgagt taagtatggg aatgagtcgt
    28561 gattataaag aagcgattca attcggttcc acttttgttc gtataggtac atcatttttt
    28621 aagtaggaga gaaccatgtc tttaaaagat agattcgata gatttataga ttattttacg
    28681 gaggatgagg attcaagtct cccttatgaa aaaagagatg agcctgtgtt tacttcagta
    28741 aattcttcac aggaaccggc tctcccaatg aatcaacctt cacagtcggc tggcacaaaa
    28801 gagaacaata tcaccagact tcatgcaaga caacaggaat tggcaaatca gagtcagcgt
    28861 gcaacggata aggtcattat agatgttcgt tatcctagaa aatatgagga tgcaacagaa
    28921 attgttgatt tattggcagg aaacgaaagt atcttgattg attttcagta tatgacagag
    28981 gtgcaggctc gtcgttgttt ggactatttg gatggagctt gtcatgtttt agctggaaat
    29041 ttgaaaaagg tagcttctac catgtatttg ttgacaccag tgaacgttat tgtaaatgtt
    29101 gaagatatcc gtttaccaga tgaagatcaa cagggtgagt tcggttttga tatgaagcga
    29161 aatagagtac gataatgatt tttttaattc gtatgattta taatgcagtg gatatttact
    29221 ccctgatttt ggtagccttc gctgtcatgt cttggtttcc aggtgcctac gaatccagtt
    29281 taggtcgttg gattgtagcg ttggtgaaac cagtgcttgc tcccttgcaa cgcctgcctt
    29341 tacagatagc gggtcttgat ttatctgttt gggttgcgat tgttttggtt cgatttttag
    29401 gagaaaacct agtgcgtttt ctggcgatga taggatgaat aaagggattt atcagcattt
    29461 ctccatagaa gatcgtccat ttcttgacaa gggaatggaa tggataaaga aggtagaaga
    29521 tagctatgct ccttttttaa ctccttttat caatcctcat caggagaagc tattaaagat
    29581 tttggccaaa acctatggtc ttgcttgtag cagtagtggg gaattcgtct cgagtgagta
    29641 tgttcgagtt ttattatacc cagattattt ccaaccagag ttttcagatt ttgaaatatc
    29701 tctccaggaa attgtgtatt ccaataaatt tgaacattta acgcatgcta agattttagg
    29761 gacagtcatc aatcaattag ggattgaacg gaaacttttt ggagatatcc tagtagatga
    29821 agaacgggcg cagattatga ttaatcagca gtttcttctt ctctttcaag atggactaaa
    29881 gaaaattggt cgtatacctg tttcgctgga ggaacgtcct ttcaccgaga aaatagataa
    29941 gctagaacag tatcgagaac tggatttatc tgtgtctagt tttcgattag atgttctttt
    30001 atcaaatgtt ttgaaactat ctaggaatca agcaaaccag ttgattgaaa agaaacttgt
    30061 ccaagtaaat tatcatgtgg tagacaaatc agattacact gttcaagttg gagacttgat
    30121 tagtgtgaga aaatttggtc gcttgagatt acttcaagat aagggacaaa cgaaaaaaga
    30181 gaagaaaaaa ataaccgtcc agttattatt aagtaagtga ggaatagaat gccaattaca
    30241 tcattagaaa taaaggacaa gacttttgga actcgattca gaggttttga tccagaagaa
    30301 gtcgatgaat ttttagatat tgtggttcgt gattacgaag atcttgtgcg tgcgaatcat
    30361 gataaaaatt tgcgtattaa gagtttagaa gagcgtttgt cttactttga tgaaataaaa
    30421 gattcattga gccagtctgt attgattgct caggatacag ctgagagagt gaaacaggcg
    30481 gcgcatgaac gttcaaacaa tatcattcat caagcagagc aagatgcgca acgcttgttg
    30541 gaagaagcta aatataaggc aaacgagatt cttcgtcaag caactgataa tgctaagaaa
    30601 gtcgctgttg aaacagaaga attgaagaac aagagccgtg tcttccacca acgtctcaaa
    30661 tctacaattg agagtcagtt ggctattgtt gaatcttcag attgggaaga tattctccgt
    30721 ccaacagcta cttatcttca aaccagtgat gaagccttta aagaagtggt tagcgaagta
    30781 cttggagaac cgattccagc tccaattgaa gaagaaccaa ttgatatgac acgtcagttc
    30841 tctcaagcag aaatggaaga attacaagct cgtattgagg tagccgataa agaattgtct
    30901 gaatttgaag ctcagattaa acaggaagtg gaaactccaa ctcctgtagt gagtcctcaa
    30961 gttgaagaag agcctctgct cgtccagttg gcccaatgta tgaagaacca gaagtagctc
    31021 cagtgcatcc gacaggtcca acaccagcta cagaaactgt tgattcagca ccgggatttg
    31081 aagcaccgca agaatctgtt acaattttat aagaaatatt ctgagaacaa tatcttatcc
    31141 ttatattccc agcgagcagg agatggtgtg agtcctgcat tccctatcga taagattatc
    31201 ctctcaaaaa ctcaagtctg aagctagtaa gatttgacgt ttcccacgtt acgggataag
    31261 agggagaaag actaaaactt tttccgaata aaggtggtac cacgattttc gtcctttttg
    31321 gaagtcgtgg tttttaattt gttattattt ataaaggaga taccatgaaa ctcaaagaca
    31381 cccttaatct tgggaaaact gaattcccaa tgcgtgcagg ccttcctacc aaagagccag
    31441 tttggcaaaa ggaatgggaa gatgcaaaac tttatcaacg tcgtcaagaa ttgaaccaag
    31501 gaaaacctca tttcaccttg catgatggcc ctccatacgc taacggaaat atccacgttg
    31561 gacatgctat gaacaagatt tcaaaagata tcattgttcg ttctaagtct atgtcaggat
    31621 tttacgcacc atttattcct ggttgggata ctcatggtct gccaatcgag caagtattgt
    31681 caaaacaagg tgtcaaacgt aaagaaatgg acttggttga gtacttgaaa ctttgccgtg
    31741 agtacgctct ttctcaagta gataaacaac gtgaagattt taaacgtttg ggtgtttctg
    31801 gtgactggga aaatccatat gtgaccttga ctcctgacta tgaagcagct caaattcgtg
    31861 tatttggtga gatggctaat aagggttata tctaccgtgg tgccaagcca gtttactggt
    31921 catggtcatc tgagtcagcc cttgctgaag cagagattga ataccatgat ttggtttcaa
    31981 cttcccttta ctatgccaac aaggtaaaag atggcaaagg agttctagat acagatactt
    32041 atatcgttgt ctggacaacg actccattta ccatcacagc ttctcgtggt ttgacggttg
    32101 gtgcagatat tgattacgtt ttggttcaac ctgctggtga agctcgtaag tttgtcgttg
    32161 ctgctgaatt attgactagc ttgtctgaga aatttggctg ggctgatgtt caagttttgg
    32221 aaacttaccg tggccaagaa ctcaaccaca tcgtaacaga acacccatgg gatacagctg
    32281 tagaagagtt ggtaattctt ggtgaccacg ttacgactga ctctggtaca ggtattgttc
    32341 atacagcccc tggttttggt gaggacgatt acaatgttgg tattgctaat aatcttgaag
    32401 tcgcagtgac tgttgatgaa cgtggtatca tgatgaagaa tgctggtcct gaatttgaag
    32461 gtcaattcta tgaaaaggta gttccaactg ttattgaaaa acttggtaac ctccttcttg
    32521 cccaagaaga aatctctcac tcatatccat ttgactggcg tactaagaaa ccaatcatct
    32581 ggcgtgcagt tccacaatgg tttgcctcag tttctaaatt ccgtcaagaa atcttggacg
    32641 aaattgaaaa agtgaaattc cactcagaat ggggtaaagt ccgtctttac aatatgatcc
    32701 gtgaccgtgg tgactgggtt atctctcgtc aacgtgcttg gggtgttcca cttcctatct
    32761 tctacgctga agatggtaca gctatcatgg tagctgaaac tattgaacac gtagctcaac
    32821 tttttgaaga acatggttca agcatttggt gggaacgtga tgccaaagac ctcttgccag
    32881 aaggatttac tcatccaggt tcaccaaacg gcgagttcaa aaaagaaact gatatcatgg
    32941 acgtttggtt tgactcaggt tcatcatgga atggagtggt ggtaaaccgt cctgaattga
    33001 cttacccagc cgacctttac ctagaaggtt ctgaccaata ccgtggttgg tttaactcat
    33061 cacttatcac atctgttgcc aaccatggcg tagcacctta caaacaaatc ttgtcacaag
    33121 gttttgccct tgatggtaaa ggtgagaaga tgtctaaatc tcttggaaat actattgctc
    33181 caagcgatgt tgaaaaacaa ttcggtgctg aaatcttgcg tctctgggta acaagtgttg
    33241 actcaagcaa tgacgtgcgt atctctatgg atatcttgag ccaagtttct gaaacttacc
    33301 gtaagattcg taacactctt cgtttcttga ttgccaatac atctgacttt aacccagctc
    33361 aagatacagt cgcttacgat gagcttcgtt cagttgataa gtacatgacg attcgcttta
    33421 accagcttgt caagaccatt cgtgatgcct atgcagactt tgaattcttg acgatctaca
    33481 aggccttggt gaactttatc aacgttgact tgtcagcctt ctaccttgat tttgccaaag
    33541 atgttgttta cattgaaggt gcccaatcac tggaacgccg tcaaatgcag actgtcttct
    33601 atgacattct tgtcaaaatc accaaactct tgacaccaat ccttcctcac actgcggaag
    33661 aaatctggtc atatcttgag tttgaaacag aagacttcgt ccaattgtca gaattaccag
    33721 aagttcaaac ttttgctaac caagaagaaa tcttggatac atgggcagcc ttcatggact
    33781 ttcgtggaca agcacaaaaa gccttggaag aagctcgtaa tgcaaaagtt atcggtaaat
    33841 cacttgaagc acacttgaca gtttatccaa atgaagttgt gaaaactcta ctcgaagcag
    33901 taaacagcaa tgtagcacaa cttttgattg tgtctgagtt gaccatcgca gaaggaccag
    33961 ctccagaagc tgcccttagc ttcgaagatg tagccttcac agttgaacgt gctgctggtg
    34021 aagtatgtga ccgttgccgt cgtatcgacc caacaacagc agaacgcagc taccaggcag
    34081 ttatctgtga ccactgtgca agcatcgtag aagaaaactt tgcggaagca gtcgcagaag
    34141 gatttgaaga gaaataagat tgaaaagtct aggcaaaatt caatttgaga agaaaagaca
    34201 actaatttta tagtctatta aacgcattgt atcacgtttt tgaacacctg atatgatgcg
    34261 ttttttattt attttaaaaa tttgcgaggt atgacttttt atactcaaca agaat
//
LOCUS       DAVHEX010000015        34080 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVHEX010000015 DAVHEX010000000
VERSION     DAVHEX010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34080
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.015"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..372
                     /locus_tag="VVL45_000719"
     CDS             <1..372
                     /locus_tag="VVL45_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU4186924.1"
                     /translation="LDNIVATKKVTIKINVKETSDTANGSLSPSNSGSGVTPMNHNHA
                     TGTTDSMPADTMTSSTNTMAGENMAASANKMSDTMMSEDKAMLPNTGETQTSMASIGF
                     LGLALAGLLGGLGLKNKKEEN"
     gene            539..1237
                     /locus_tag="VVL45_000720"
     CDS             539..1237
                     /locus_tag="VVL45_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4186925.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            1234..2286
                     /locus_tag="VVL45_000721"
     CDS             1234..2286
                     /locus_tag="VVL45_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEU4186926.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKDQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            2481..3092
                     /gene="rpsD"
                     /locus_tag="VVL45_000722"
     CDS             2481..3092
                     /gene="rpsD"
                     /locus_tag="VVL45_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEU4186927.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            3278..3433
                     /locus_tag="VVL45_000723"
                     /pseudo
     CDS             3278..3433
                     /locus_tag="VVL45_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(3537..3674)
                     /locus_tag="VVL45_000724"
                     /pseudo
     CDS             complement(3537..3674)
                     /locus_tag="VVL45_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            3754..4104
                     /locus_tag="VVL45_000725"
     CDS             3754..4104
                     /locus_tag="VVL45_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4186928.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKALADDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETADKDVLENSQEELQKAVLFLADEKGSEHTAAELI
                     DNLKEVIAKLKANA"
     gene            4423..5352
                     /locus_tag="VVL45_000726"
     CDS             4423..5352
                     /locus_tag="VVL45_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU4186929.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            5366..6289
                     /locus_tag="VVL45_000727"
     CDS             5366..6289
                     /locus_tag="VVL45_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU4186930.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            6547..8022
                     /locus_tag="VVL45_000728"
     CDS             6547..8022
                     /locus_tag="VVL45_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HEU4186931.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            8294..9280
                     /locus_tag="VVL45_000729"
     CDS             8294..9280
                     /locus_tag="VVL45_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEU4186932.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEELWFKIDEENEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            9555..10406
                     /locus_tag="VVL45_000730"
     CDS             9555..10406
                     /locus_tag="VVL45_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HEU4186933.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDVPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDGSKPEDYESIARLDYAKFLEVLPPSFYHQLDANQIEIQPILV
                     QDFKTLA"
     gene            complement(10487..11551)
                     /locus_tag="VVL45_000731"
     CDS             complement(10487..11551)
                     /locus_tag="VVL45_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4186934.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIADLDTPIGK
                     NTQVEEEVSQAEVELESQQEEKIETPEGGEARTEIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIVSILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(11614..12525)
                     /locus_tag="VVL45_000732"
     CDS             complement(11614..12525)
                     /locus_tag="VVL45_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HEU4186935.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHTLTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILRLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAEDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            complement(12518..13111)
                     /locus_tag="VVL45_000733"
     CDS             complement(12518..13111)
                     /locus_tag="VVL45_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEU4186936.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEVAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTVLAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVARFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            complement(13098..13424)
                     /locus_tag="VVL45_000734"
     CDS             complement(13098..13424)
                     /locus_tag="VVL45_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU4186937.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWTLYTDTINGIIEGS
                     IRHDKN"
     gene            complement(13563..14729)
                     /locus_tag="VVL45_000735"
     CDS             complement(13563..14729)
                     /locus_tag="VVL45_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HEU4186938.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTQLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TAMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPAVAALLLIDILMTVALFTVDRADKIL"
     gene            14787..15944
                     /locus_tag="VVL45_000736"
     CDS             14787..15944
                     /locus_tag="VVL45_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU4186939.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTETFVMNNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREHGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGLGTIVGLIRGFKWKKEYKRTIIYLDKISQINKNML"
     gene            15986..17860
                     /locus_tag="VVL45_000737"
     CDS             15986..17860
                     /locus_tag="VVL45_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEU4186940.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDILSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSDLELVGILDKDAKKKGQKLGGIPVLGSYDDLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVAKAVDEAKVPKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLEAIDQKIEEFRTLSGDELKQAIIAFANQTTHVEAVSLLVKE
                     "
     gene            18375..18545
                     /locus_tag="VVL45_000738"
     CDS             18375..18545
                     /locus_tag="VVL45_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HEU4186941.1"
                     /translation="MKYFEVELENPDEFLKLQTEDFVKANRLLLRKIIQSVTVYEENF
                     VISFKSGIELEV"
     gene            18931..20148
                     /gene="mef(A)"
                     /locus_tag="VVL45_000739"
     CDS             18931..20148
                     /gene="mef(A)"
                     /locus_tag="VVL45_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(A)"
                     /protein_id="HEU4186942.1"
                     /translation="MEKYNNWKRKFYAIWAGQAVSLITSAILQMAIIFYLTEKTGSAM
                     VLSMASLVGFLPYAILGPAIGVLVDRHDRKKIMIGADLIIAAAGAVLAIVAFCMELPV
                     WMIMIVLFIRSIGTAFHTPALNAVTPLLVPEEQLTKCAGYSQSLQSISYIVSPAVAAL
                     LYSVWDLNAIIAIDVLGAVIASITVAIVRIPKLGNQVQSLEPNFIREMKEGVVVLRQN
                     KGLFALLLLGTLYTFVYMPINALFPLISMEHFNGTPVHISITEISFAFGMLAGGLLLG
                     RLGGFEKHVLLITSSFFIMGTSLAVSGILPPNGFVIFVVCCAIMGLSVPFYSGVQTAL
                     FQEKIKPEYLGRVFSLIGSIMSLAMPIGLILSGFFADKIGVNHWFLLSGILIIGIAIV
                     CQMITEVRKLDLK"
     gene            20268..21731
                     /gene="msr(D)"
                     /locus_tag="VVL45_000740"
     CDS             20268..21731
                     /gene="msr(D)"
                     /locus_tag="VVL45_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(D)"
                     /protein_id="HEU4186943.1"
                     /translation="MELILKAKDIRVEFKGRDVLDINELEVYDYDRIGLVGANGAGKS
                     TLLRVLLGELTPPGCKMNRLGELAYIPQLDEVTLQEEKDFALVGKLGVEQLNIQTMSG
                     GEETRLKIAQALSAQVHGILADEPTSHLDREGIDFLIGQLKYFTGALLVISHDRYFLD
                     EIVDKIWELKDGKITEYWGNYSDYLRQKEEERKSQAAEYEQFIAERARLERAAEEKRK
                     QARKIEQKAKGSSKKKSTEDGGRLAHQKSIGSKEKKMYNAAKTLEHRIAALGKVEAPE
                     GIRRIRFRQSKALELHNPYPIVGAEINKVFGDKALFENASFQIPLGAKVALTGGNGIG
                     KTTLIQMILNHEEGISISPKAKIGYFAQNGYKYNSNQNVMEFMQKDCDYNISEIRSVL
                     ASMGFKQNDIGKSLSVLSGGEIIKLLLAKMLMGRYNILIMDEPSNFLDIPSLEALEIL
                     MKEYTGTIVFITHDKRLLENVADVVYEIRDKKINLKH"
     gene            complement(21849..22148)
                     /locus_tag="VVL45_000741"
     CDS             complement(21849..22148)
                     /locus_tag="VVL45_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4186944.1"
                     /translation="MNQLEFQRNHLQMDYYSESYQDFERDFYRYSNMNIPLTFLTDDI
                     LKTMATSRKNYFVLNKEKSRDNRDHFFIFEVSTVDENPLIYHYTYKKTTIYLAEK"
     gene            complement(22135..22503)
                     /locus_tag="VVL45_000742"
     CDS             complement(22135..22503)
                     /locus_tag="VVL45_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4186945.1"
                     /translation="MIDRSYLPFQSAREYQDTKMQKWMGFFLSEHASALSDDTNKVTY
                     MSDLSLEKKLLLLSQVYAGQLRTRIQVIEKNKRVSYTGTIPSLTKDFILIKTTTGHIN
                     LKLKDIISIELVEEVLYESA"
     gene            complement(22516..22851)
                     /locus_tag="VVL45_000743"
     CDS             complement(22516..22851)
                     /locus_tag="VVL45_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imsC-like protein"
                     /protein_id="HEU4186946.1"
                     /translation="MKQEKNTVQFSEIRSKGCNDIEMLERFLHGIVETATSKLRQRKL
                     KTTEISIRLVHAKSENRLPLEFTFSIKPTSSSVIIYTEVINRFKECYTGGGIQGFTIQ
                     FDKNTLASA"
     gene            complement(22848..23093)
                     /locus_tag="VVL45_000744"
     CDS             complement(22848..23093)
                     /locus_tag="VVL45_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4186947.1"
                     /translation="MRQIGVSYSGFVDESYTLLSLFDDVEQIEKDNRLQTAIDVVREQ
                     FGFLAIQKGTVLTEGSRNIERSKLIGGHSAGGLEGLK"
     gene            complement(23454..24086)
                     /locus_tag="VVL45_000745"
     CDS             complement(23454..24086)
                     /locus_tag="VVL45_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEU4186948.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTHTFKELGVPSPDAKTIR
                     GFMGPPLENSFATCLPKEQISEAVQIYRSYYKAKGIHEAQLFPHIVDLLEKLSSKYPL
                     YITTTKNTPTAQDMTKNLGIFHFFDGIYGSSPEAPHKADVIRQALHTHQLAPEQAIII
                     GDTKFDMQGAQETGIQKLAVTWGFGEQADLLNYQPDYIAHKPIEVLEYFQ"
     gene            complement(24108..24980)
                     /gene="sdaAA"
                     /locus_tag="VVL45_000746"
     CDS             complement(24108..24980)
                     /gene="sdaAA"
                     /locus_tag="VVL45_000746"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEU4186949.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIKNGKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAA
                     TPTGRRLQKEIFGE"
     gene            complement(24989..25660)
                     /gene="sdaAB"
                     /locus_tag="VVL45_000747"
     CDS             complement(24989..25660)
                     /gene="sdaAB"
                     /locus_tag="VVL45_000747"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEU4186950.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            25902..26474
                     /locus_tag="VVL45_000748"
     CDS             25902..26474
                     /locus_tag="VVL45_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU4186951.1"
                     /translation="MKSTTKKIKTTLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNGDYSAE
                     NQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            complement(26527..26847)
                     /locus_tag="VVL45_000749"
     CDS             complement(26527..26847)
                     /locus_tag="VVL45_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU4186952.1"
                     /translation="MQRLEVYKNYQHLYDLRMTILLNLSTLYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLAICYVRIGICRDDAKLIQKGFSLLELTEETSMLSHLKKEVETHYQ
                     PKKL"
     gene            27278..27565
                     /locus_tag="VVL45_000750"
     CDS             27278..27565
                     /locus_tag="VVL45_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEU4186953.1"
                     /translation="MRKLVKIGVASLMVCGILATTNALAVWVEGGQWNYGVGWTGTFG
                     YSDYLHSTRYHTATVRHGGRTSKDYAKPEAWARASLTKIPPTGMEYFYGFE"
     gene            27617..29725
                     /locus_tag="VVL45_000751"
     CDS             27617..29725
                     /locus_tag="VVL45_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU4186954.1"
                     /translation="MKKISNFCMLLLLLCTTFFVFNVNYTREVVRIQEMGKTVDSLDL
                     YLKDINEPAASVLRFFEDVSKEYKVSIIKTDSGDEVVKSGVFDKDTFPYQEFGISSLD
                     FTTDGEGVYSNKEISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTITSTQEM
                     DKDRIVQKWSDFFKIDQATLLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQP
                     MMEMKRVGVQKLLGFQDRAVLADVVKGNLYLLLGGALVINLGVFFLLDYKPKDLFPML
                     WLSHFLLLQLYLFISWLTYLLIQKMTISSLLKGFSSFKFGLIFNYVMKIGTTILLTAL
                     LIGVGRSLEQENKELAYQQQWVSQGNYLTLETFKLNDNLWQEELAGSGKSTDYFYRFY
                     QDLVEKTQAGYVQSSSLPVKNFVQSEQIQQYQLTDTVDVYYANRNFLKSKGFKLPNTG
                     IKKVILMPASTKGEEDKNQLLGKLIAFHSMKYEEQQKRTIEEMDVEIAYYEGDWSFFP
                     YSDKRKENLSNPIISLVNDSDMMWDEKASLSTTGLNNPIKIENTVQHQKEITELVEKL
                     SDGNYLKFSSIQAIQQEKVDSYRDAVRNFNLLFALFGLLSMMISYFLLVTTFLLKRRD
                     IITKKFMGWKLVDRYRPLLVLLLLGYSFPLLVLIFFAHAFLPLLLFAGFTCLDILFVL
                     GLASRMEKRSLVELLKGGIL"
     gene            29722..30363
                     /locus_tag="VVL45_000752"
     CDS             29722..30363
                     /locus_tag="VVL45_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4186955.1"
                     /translation="MIELKNITKTIGGKVILDNLSLRIDQGDLVAIVGKSGSGKSTLL
                     NLLGLIDGDYSGRYEIFGQTNLAVNSAKSQTIIREHISYLFQNFALIDDETVEYNLML
                     ALKYVKLPKKDKLKKVEEILERVGLSATLHQRVSELSGGEQQRIAVARAILKPSQLIL
                     ADEPTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRIIELGEGK"
     gene            30570..31376
                     /locus_tag="VVL45_000753"
     CDS             30570..31376
                     /locus_tag="VVL45_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU4186956.1"
                     /translation="MNKMKKVLMTMFGLVMLPLLFACSNNQSAGIEAIKSKGKLVVAL
                     NPDFAPFEYQKVVDGKNQIVGSDIELAKAIATKLGVELELSPMSFDNVLASVQSGKAD
                     LAISGVSKTDERSKVFDFSIPYYTAKNKLIVKKSDLTTYQSVNDLAQKKVGAQKGSIQ
                     ETMAKDLLQNSSLVSLPKNGNLITDLKSGQVDAVIFEEPVSKGFVENNPDLAIADLNF
                     EKEQDDSYAVAMKKDSKKLKEAVDKTIQKLKESGELDKLIEDAFKASIEK"
     gene            31342..31812
                     /locus_tag="VVL45_000754"
     CDS             31342..31812
                     /locus_tag="VVL45_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEU4186957.1"
                     /translation="MRMPLKHPLKNRKKEKNMSKEKVILAYSGGLDTSVAITWLKKDY
                     DVVSVCMDVGEGKDLDFIHDKALKVWAVESYVIDVKDEFATDYVLVAHQSHAYYEQKY
                     LLVSALSRPLISKKLVEIAHQIGATTIAHGCTGKGNDQVEYQIAVAKKANEAKK"
     gene            complement(31999..32862)
                     /locus_tag="VVL45_000755"
     CDS             complement(31999..32862)
                     /locus_tag="VVL45_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU4186958.1"
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGGVLSRTSLSKIESGKTTPK
                     YENMEFLLRQINMSFEEFEYICQLYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQD
                     YLKTHHDLPIEEIRDMLEVVIYLRQHGAGELSDHAEQVVKKLWRKIEKQDTWYESDLK
                     ILNTILFSFPIEYLHLITGKILQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMC
                     KQICYTLLEDAKNKKSYDRLAICYVRIGICTDDSKLIQKGFSLLELTEETSMLSHLKK
                     EVETHYQPKKL"
     gene            33328..33666
                     /locus_tag="VVL45_000756"
     CDS             33328..33666
                     /locus_tag="VVL45_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HEU4186959.1"
                     /translation="MLKKYPCTMQHDQSDCAAAVVSTVLLSYKKELSIMKIREIIGTD
                     MYGTTVSGIVSGLNKLNFTVKAVRVALEDLTPKLTFPAILQVKNDLGQNHFVVLHSIK
                     EKINGTRITK"
     gene            33695..>34080
                     /locus_tag="VVL45_000757"
     CDS             33695..>34080
                     /locus_tag="VVL45_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4186960.1"
                     /translation="MYLDGGAYLSKSACQGICAALAMSPGTFIALTGAAVLTKKLINY
                     IKVGGLGGWLIGAAAGVLALIFLIKICVDTTIKVLLNRIFKASKVMKRRIFSLFVLDL
                     MVYIFLGYVLVIQKDVYLFSILIIFS"
BASE COUNT        10412 a         6017 c         7326 g        10325 t
ORIGIN      
        1 ttggacaaca tcgtagcaac taaaaaagtc actattaaga taaatgttaa agaaacatca
       61 gacacagcaa atggttcatt atcaccttct aactctggtt ctggcgtgac tccgatgaat
      121 cacaatcatg ctacaggtac tacagatagc atgcctgctg acaccatgac aagttctacc
      181 aacacgatgg caggtgaaaa catggctgct tctgctaaca agatgtctga tacgatgatg
      241 tcagaggata aagctatgct accaaatact ggtgagactc aaacatcaat ggcaagtatt
      301 ggtttccttg ggcttgcgct tgcaggttta ctcggtggtc taggtttgaa aaacaaaaaa
      361 gaagaaaact aatcagctaa ggaaataaat gatggatagt gggctgacta agactagttt
      421 aacaactcaa tcagcaatca ggactttctt tcaatagcag attaaaatca tcgtaaaaca
      481 ataaaaatag tgttatactt aaagcagtat agcactgttt ttatcaaagg agagacagat
      541 gggaaagaca attttactcg ttgacgacga ggtagaaatc acagatattc atcagagata
      601 cttaattcag gcaggttatc aggtcttggt agcccatgat ggactggaag cgctagagct
      661 gttcaagaaa aaaccgattg atttgattat cacagatgtc atgatgcctc ggatggatgg
      721 ttatgattta atcagtgagg ttcaatactt atcaccagag cagcctttcc tatttattac
      781 tgctaagacc agtgaacagg acaagattta cggcctgagc ttgggagcag atgattttat
      841 tgctaagcct tttagcccac gtgagctggt tttgcgtgtc cacaatattt tgcgccgcct
      901 tcatcgtggg ggcgaaacag agctgatttc ccttggcaat ctaaaaatga atcatagtag
      961 tcatgaagtt caaataggag aagaaatgct ggatttaact gttaaatcat ttgaattgct
     1021 gtggatttta gctagcaatc cagagcgagt tttctccaag acagacctct atgaaaagat
     1081 ctggaaagaa gactacgtgg atgacaccaa taccttgaat gtgcatatcc atgctcttcg
     1141 acaggagctg gcaaaatata gtagtgacca aacgcccact attaagacag tttgggggtt
     1201 gggatataag atagagaaac cgagaggaca aacatgaaac taaaaagtta tattttggtt
     1261 ggatatatta tttcaaccct cttaaccatt ttggttgttt tttgggctgt tcaaaaaatg
     1321 ctgattgcga aaggcgagat ttactttttg cttgggatga ccatcgttgc cagccttgtc
     1381 ggtgctggga ttagtctctt tctcctattg ccagtcttta cgtcgttggg caaactcaag
     1441 gagcatgcca agcgggtagc ggccaaggat tttccttcaa atttggaggt tcaaggtcct
     1501 gtagaatttc agcaattagg gcaaactttt aatgagatgt cccatgattt gcaggtaagc
     1561 tttgattcct tggaagaaag cgaacgagaa aagggcttga tgattgccca gttgtcgcat
     1621 gatattaaga ctcctatcac ttcgatccaa gcgacggtag aagggatttt ggatggtatt
     1681 atcaaggagt cggagcaagc tcattatcta gcaaccattg gacgccagac ggagaggctc
     1741 aataaactgg ttgaggagtt gaattttttg accctaaaca cagctagaaa tcaggtggaa
     1801 actaccagta aagacagtat ttttctggac aagctcttaa ttgagtgcat gagtgaattt
     1861 cagtttttga ttgagcagga gagaagagat gtccacttgc aggtaatccc agagtctgcc
     1921 cggattgagg gagattatgc taagctttct cgtatcttgg tgaatctggt cgataacgct
     1981 tttaaatatt ctgctccagg aaccaagctg gaagtggtgg ctaagctgga gaaggaccag
     2041 ctttcaatca gtgtgaccga tgaagggcag ggtattgccc cagaggattt ggaaaatatt
     2101 ttcaaacgcc tttatcgtgt cgaaacttcg cgtaacatga agacaggtgg tcatggatta
     2161 ggacttgcga ttgcgcgtga attggcccat caattgggtg gggaaatcac agtcagcagc
     2221 cagtacggtc taggaagtac ctttaccctc gttctcaacc tctctggtag tgaaaataaa
     2281 gcctaaaacc cctttacaaa tccagctatt catggtagaa tagattttgt gtgaaatatc
     2341 agcaggaaag catgaagctc gtcaacaggt gtcttatgac aagtaacctt ggctgtttag
     2401 gcgaagggca tctgcacgaa tcagggcttt ctaagtgact atttccaccg aaatattatt
     2461 tatatcagga ggacattcat atgtcacgtt atacaggacc atcttggaaa caagctcgtc
     2521 gccttggcct ttcacttaca ggtacaggta aagaattggc acgtcgtaac tacgtaccag
     2581 gacaacacgg accaaacaac cgttctaaat tgtcagaata cggtttgcaa ttggctgaaa
     2641 aacaaaaact tcgtttcact tacggtgtag gtgaaaaaca attccgtaac ttgttcgtac
     2701 aagctacaaa aatcaaaggc ggaatcctag gtttcaactt tatgcttctt ttggaacgtc
     2761 gtttggataa cgttgtttac cgtcttggtc tcgcgactac tcgtcgtcaa gctcgtcaat
     2821 tcgtaaacca cggtcacatc cttgttgacg ggaaacgcgt tgatatccca tcataccgcg
     2881 taactccagg tcaagtgatc tcagttcgtg aaaaatcatt gaaagttcca gcaatccttg
     2941 aagcagtaga agctactctt ggacgtccag cattcgtatc attcgacgct gaaaagttgg
     3001 aaggttcatt gactcgcttg ccagaacgcg acgaaatcaa cccagaaatc aacgaagcac
     3061 ttgtcgttga attctacaac aaaatgttgt aatattttat tgaataagat aggctttgag
     3121 gccttgatat taagcacttt gggacgttct cccttagtgc ttttttgatt tctcttagta
     3181 tccagctata atcgtttaga cataactaga ccgatatagt ccaaagtgat atagtaaaat
     3241 gaaccaaaaa tagtacacaa tgtggtataa tctttttatg gcatattcaa tagattttcg
     3301 taaaaaagtt ctctcttatt gtgagcgaac aggtagtata acagaagcat cacacgtttt
     3361 ccaaatctca cgtaatacca tttatggctg gttaaagcta aaagagaaaa caggagagct
     3421 aaactaccaa gtatagtgta ttgaatctat aacagtacac cttggctgct aaaatatttc
     3481 tataaattaa tttgactttc ctgatagagt tgttcacatc ttatttcaaa tcactataaa
     3541 agctctataa tctctttata agatttgccc atcagacgaa atagaacgat ttgaaggcgt
     3601 ttatgatatt tagctgtacg agaatctttt aaaagtgttt tgatggtttg gatttcttct
     3661 ttagttgatt tcatatcact gttatataat gctttttgat ttgagtctag tattagtaag
     3721 cagactcaaa ccttagaaaa tcttttgaga tcattgacag gtgtatcgaa tcctaccaca
     3781 gattcagcac gtctggtttt ggcagaggct aagaaagctc ttgcagatga tagtttgaca
     3841 gagcaaggtt tacgtgatat cttgcaaaca gtcaaagatg ccattgcttc cctagagtcc
     3901 attaaggaaa gccagtcagc aactaaggat ggaggacaaa cagcgggcaa agaaacagca
     3961 gataaggatg tccttgaaaa ctctcaagaa gaacttcaaa aagcagtttt gttcttggca
     4021 gatgaaaaag gctctgagca tactgcagca gagttgattg ataatcttaa agaggttatt
     4081 gctaagttga aagcaaacgc ctaataacat attaaaaatc tccagattag gaactatcgc
     4141 tgagttccct agtctggaga tttttcagta taccccgttg ttggatgctt ataacagagt
     4201 gacgccatca ctgggctaat agagataaaa taaacaattg atttaggaca tttgtttgat
     4261 agtggtgata aagttttaaa tctgctacat aatttgtgga gttttttcta taattaggag
     4321 tttaacctaa cctgcaaata ggagtatact aataatgtaa tcgttatcaa aagtctaaaa
     4381 aagaattttt agatggatat caaaataaaa agggaggaaa ttatgaaaaa gttttcaaaa
     4441 acattgagag acaactggat ctttctcttg atggttttgc caggggcact ctggttgatt
     4501 ctattctttt acattccagt atttgggaac gtggttgcct tcaaagacta ccacatgacc
     4561 agtaatggtt tcatagatag tatcataaat agtaaatggg tcggactcga taattttaga
     4621 ttcttattta gttcaagaga cgcctttatt atcacacgaa atactgtcct ctacaatctt
     4681 ggctttatct ttctaggttt agttgtatct gtagggattg ccattatcct cagcgagctc
     4741 cgttctaaga gaatggtgaa gatttttcaa acttctatgt tgttccctta cttcttgtct
     4801 tgggttatca tcagtttctt tacagatgcc ttcctaaata ttgataaagg ggtgttcaat
     4861 catctattgg aaagtcttgg tctcaaagaa gtcaatttct acgctgacct gggcatctgg
     4921 ccctatctcc tacttttcct aggtatttgg aaaggctttg gatatagcag tgtcatgtac
     4981 tatgcgacga tcatgggaat tgatccaacc tactacgaag cagcgacagt ggacggagct
     5041 agcaagtggc aacgtattcg caacgtaacc attcctcagt tgactccgct tgtaactgta
     5101 ttgaccatcc ttgcagtcgg aaatatcttc cgcgcagact tcggtctctt ctatcaaatc
     5161 ccacacaatg ctggtcagct ttacaatgta accaacgttt tggacgtata tgtctttaat
     5221 ggtttgactc agacagcaga tatcggtatg gctgcagcag ccggtcttta ccaatccgtt
     5281 gttggtttga ttctggttat cctatcaaac ttgcttgcaa gacgagtcga tccaaactca
     5341 gctttgttct agaaaggagg agaatatggc aaaaaagaaa attaaaaaag aaaaaattga
     5401 taatgtcggc attcactcct tcagtaagaa agcagatatc ttcttcagta tcatttcggg
     5461 tttgattgcc ctctcttgta tcctaccatt tgtattcgtt atcattattt cagtgacaga
     5521 cgagaaaagc ctcctccaat acggctatag cttcttccca tcccaatttg gcttagacgg
     5581 ttttgagttt ttggcacagt ttaaggataa aatcctacaa gccctcttca tctctgtctt
     5641 tgtaaccgta gttgggacat tgacaaatgt ctttatcaca acaacctatg cctacgctat
     5701 ttcacggaca acctttaagt atcgcagatt ctttaccatc ttcgtccttc tcagtatgtt
     5761 gttcaacgct ggtttggtac caggttatat catggtgacc cgtgtacttc agcttggtga
     5821 cactgtttgg gccttgattg ttccaatgct tctctcacca ttcaatatca tcttgatgcg
     5881 ttccttcttc aagaagacca ttccagaagc cattctcgaa tcggctcgta tcgatggtgc
     5941 cagtgaggcc cggattttct tccaaatctg tttgccattg tcactaccag gtatcgcaac
     6001 catcacgctt ttgacagctc ttggtttctg gaatgactgg ttcaacgccc ttctttacat
     6061 caagagtgac aacttgtatc cattgcaata tttgctcatg caaatccaac aaaatatgga
     6121 ctacattgcc aaagcggtcg gcttgactgg tcaactggga gttgctctac cgaaagaaac
     6181 aggtcgtatg gccatggttg tggttgcaac ccttccaatc gcgattttgt atccattttt
     6241 ccaacgctac tttgtaaaag gtttgactat cggtggtgtg aaagaatagt gcttattgag
     6301 aaataacatt tctcagttcc caacttccct tgtgtgaagt taatactctt cgaaaatcaa
     6361 attcaaaccg cgtcaacgtc gccttgccgt agatatatgt aactgacttc gtcagtctta
     6421 tctacaacct caaagcagtg ctttgagcag cctgcggcta gttttctagt ttgctctttg
     6481 attttcattg agtataagat cattacattg tttataagtt taaaaataaa aaaaggagtt
     6541 tttatcatga aaaactggaa aaaatatgct tttgcatctg ctagtgtagt cgctctggct
     6601 gcaggtcttg ctgcttgtgg aaatttgaca ggtaacagca aaaaagctgc tgattcaggt
     6661 gacaaacctg ttatcaaaat gtaccaaatc ggtgacaaac cagacaactt ggatgaattg
     6721 ttagcaaatg ccaacaaaat cattgaagaa aaagttggtg ccaaattgga tatccaatac
     6781 cttggctggg gtgactatgg taagaaaatg tcagttatca catcatctgg tgaaaactat
     6841 gatattgcct ttgcagataa ctatattgta aatgctcaaa aaggtgctta cgctgacttg
     6901 acagaattgt acaaaaaaga aggtaaagac ctttacaaag cacttgaccc agcttacatc
     6961 aagggtaata ctgtaaatgg taagatttac gctgttccag ttgcagccaa cgttgcatca
     7021 tctcaaaact ttgccttcaa cggaactctc cttgctaaat atggtatcga tatttcaggt
     7081 gttacttctt acgaaactct tgagccagtc ttgaaacaaa ttaaagaaaa agctccagac
     7141 gtagtaccat ttgctattgg taaagttttc atcccatctg ataactttga ctacccagta
     7201 gcaaacggtc ttccattcgt tatcgacctt gaaggcgata ctactaaagt tgtaaaccgt
     7261 tacgaagtgc ctcgtttcaa agaacacttg aagactcttc acaaattcta tgaagctggc
     7321 tacattccaa aagacgtcgc aacaagcgat acttcctttg accttcaaca agatacttgg
     7381 ttcgttcgtg aagaaacagt aggaccagct gactacggta acagcttgct ttcacgtgtt
     7441 gccaacaaag atatccaaat caaaccaatt actaacttca tcaagaaaaa ccaaacaaca
     7501 caagttgcta actttgtcat ctcaaacaac tctaagaaca aagaaaaatc aatggaaatc
     7561 ttgaacctct tgaatacgaa tccagaactc ttgaacggtc ttgtttacgg tccagaaggc
     7621 aagaactggg aaaaaattga aggtaaagaa aaccgtgttc gcgttcttga tggctacaaa
     7681 ggaaacactc acatgggtgg atggaacact ggtaacaact ggatccttta catcaacgaa
     7741 aacgttacag accaacaaat cgaaaattct aagaaagaat tggcagaagc taaagaatct
     7801 ccagcgcttg gatttatctt caatactgac aatgtgaaat ctgaaatctc agctattgct
     7861 aacacaatgc aacaatttga tacagctatc aacactggta ctgtagaccc agataaagct
     7921 attccagaat tgatggaaaa attgaaatct gaaggtgcct acgaaaaagt attgaacgaa
     7981 atgcaaaaac aatacgatga attcttgaaa aacaaaaaat aatcagctaa attgatttcg
     8041 tgtattcatt cctaactcct aaaataagaa tcactgcttt cctcaggttg gtctggggag
     8101 ggcagtggtt tttttggcac aaggatatag atatccatgt tttctcttgt ccttatttta
     8161 accattcagg aaatttcgag aaccctctac aatttccagt caaataaatt gcattcgttt
     8221 tctcaagcag gtatactagt atggataaat aaaaaattta gaaaatttaa gaatagaaaa
     8281 gagaacaaat cttatggcaa aagatattcg tgtcttactt tactaccttt atactccaat
     8341 tgaaaatgca gagcaatttg ctgcagacca cttggctttc tgtaaatcaa tcggccttaa
     8401 aggccgtatc ctagtcgctg acgagggaat taacggaaca gtttcaggtg actatgaaac
     8461 aactcaaaaa tacatggact acgttcacag ccttccagga atggaagaac tttggttcaa
     8521 gattgacgaa gaaaatgaac aagccttcaa gaagatgttt gttcgctaca agaaagaaat
     8581 tgtccacctt ggtttggaag acaacgactt tgacaatgac atcaacccac ttgaaacaac
     8641 aggtgcttac ttgtctccaa aagagttcaa agaagcgctt cttgataaag ataccgttgt
     8701 ccttgacaca cgtaacgatt atgagtacga cctaggacat ttccgtggag ctattcgccc
     8761 agatattcgc aacttccgtg agttaccaca atgggtccgt gataacaagg aaaaattcat
     8821 ggacaagcgt gtcgtggttt actgtacagg tggcgttcgc tgtgagaaat tctcaggctg
     8881 gatggtccgt gaaggctaca aagatgtcgg ccaattgcac ggaggaatcg caacttacgg
     8941 taaagaccca gaagttcaag gtgagctttg ggatggaaaa atgtacgtat ttgacgagcg
     9001 tatcgccgtt gatgtcaacc atgtcaaccc aaccatcgta gggaaagact ggtttgatgg
     9061 aacaccatgt gaacgttatg tcaactgtgg aaatcccttc tgtaaccgtc gtatcttgac
     9121 atcagaagaa aatgaagaca agtaccttcg tggatgctca cacgagtgcc gtgttcaccc
     9181 acgtaaccgc tatgtttcaa aaaatgaatt gacacaagct gaagtgattg agcgcctagc
     9241 cgctatcggt gaaagcttgg atcaggcagc tactgtataa gatcaaacag tccttagggg
     9301 ctgtttttct atgctttgta ctcaaaaatc taatactcaa tgaaaatcaa agagcaaact
     9361 aggaagctag ctgcaggctg ctcaaagcac tgctttgagg ttgtagataa gactgacgaa
     9421 gtcagttaca tatatctacg gcaaggcgaa gctgacgcgg tttgaagaga ttttcgaaga
     9481 atataaagtt tgtttttgta tctttcagaa aaataaggta cactgtatgt aaacgatttc
     9541 aaaggagtcc agttatggcg aaaacatttt ttattccaaa taaacagagc attttaggag
     9601 aacaagagat tttgaatgcc aagtcgatct tggccttgct agacggtttg gagtcacata
     9661 gctatgatgt agtctatctc cgtcagcctc ttaatcgtct cgagtatatt gagtgtgcga
     9721 tagtggggca atcacaattt ctctttaagg tcagttatgc tgatggtcaa aaggcttatc
     9781 gtgttgatgt tcctgaccta ctaacaaaga cagactggca gattatcaag tcatttttag
     9841 atgctttgct tgcttataca gggactgata ttgaaggact agatggtttt gattttgaag
     9901 cttatttcca agcaagtatt caagcctatc tagcagaccc tgtagctcgt tttacgattt
     9961 gccaaggaat ttttaatcct attttcttta gtcgtgagaa cttgaaaagc tttttagagg
    10021 cagatggctt ggctcagttt gaagcgcgtg tgcgtgcggt tcaagagaca gatgcctact
    10081 ttgcgagagt ttccttctat caggatggag aaggaaaagt gcatggcgtt taccatctag
    10141 ctcaaggagt caagacagtt ttaccgagag aaccgtttgt tcctgcagcc tatattgagc
    10201 aattggtgga taaggaagtc cagtgggaga ttgacttggt tcaaatcaca ggagacggct
    10261 ctaaaccaga agactatgaa tccatagctc gcttggacta tgcaaaattc ttagaggtgt
    10321 tacccccatc tttttaccac cagctagacg ccaatcaaat agaaatacaa cccatcctag
    10381 tacaagattt taaaacatta gcataagaaa agtaaagcag aagcaggtca atcgacttgc
    10441 ttttttgaca tagaaaaaat cctgccaaga atgacaggat tgaattttaa agaaaggcca
    10501 agatacgaag ataatctcca atcagtgcca cttcagctac aaagaagagg aggataataa
    10561 ctccgttcac aaggacagac aagaataatt gatagaagga gtcggtttca cttgcttgac
    10621 ttggtcttgt aatgatatgg agactggcaa gcagaatgat tccaatgcta atcacacaca
    10681 agagggctgt aaatcgtagg ctatcaaaga aagcaaagaa actagcaata gcagtgagga
    10741 agattggaat tgccaagagt tgactatatt gttggagaac cttgtctagc gtccagtcct
    10801 tttcctggtg gataaatcgt ctcacaacga aactacccaa gaggaatgaa aagaagaaga
    10861 gtgttgtcgc tactaggata gagacgatag aaaaaagagt taaaggagct agctgctcag
    10921 ggaagcgact gttaatgctt gctatatgtc catagtaagc atgtttgatg tgatagatac
    10981 taaagaaaaa ggaagatgca gaaaacagaa tgagcaagag aaaggctgtg taactgtgtg
    11041 tgatacttgt ttccaactta cttgtaggag atttgatcgc ttccactagc caagaccaaa
    11101 aatcaagcac ttgctctttc catttatccc tagattttgg agcttggtcg gggatataag
    11161 gactttctaa agatttactg ataagaagtg gctctttcgt ggttgctttt tgctgaggaa
    11221 gagcttcttg gctctcttca gctatagtga ctttttctgt ttctttagaa aggtctggct
    11281 cttcttcagt agaattagat gccttctttt cttctatttc tgttctcgct tcaccgcctt
    11341 caggagtttc aattttctct tcttgctggc tttccagttc gacttcagct tgagagactt
    11401 cctcctctac ttgagtattt tttccaattg gtgtatcgag atcggctatc gtttcttcag
    11461 ccttgtctgc aacctcttga gcttgctctt caggcttgtt cttgcttgtt gtttttacaa
    11521 aatcattact ttcaaaccat tcttgtttca tggtagaacc tcctttttag ttagataaat
    11581 atgcttccat agtagcagat gtaagcgttt ttgtcaacgt ctgcttggtg tggatattag
    11641 atcaatatta tcatcagatc tcgcaatgag ttgatccttg acatcggttt tttcagtttt
    11701 gtaagggttg cttaattccg tacctcttga ttcaggcttt tctcttgtga attggaagat
    11761 agaaccatag ttgcttgaga tgtcccagtt aattcgttgg ctttctttct ggtctaggat
    11821 gattctgaga taatcttcgg cagtcagttc aaccttacca tggacttgga tattttcagc
    11881 gtggaagtga ttctctgttg actctagctg actatctgta agaactgtat caaagatatt
    11941 aacgatattg ggcgttgtga gtttactgtt tttgatacga cttccttcaa ttcggaggat
    12001 atagctgttt gtattgaggg tcgcattttc aaggctagca tttatgatgg tggtttgtcc
    12061 gcgattggct gagatgttga tcccttttag agttctccct tttggtagtc ggagaataac
    12121 ttcttcaaaa cgactagagt agctacttgc gatatgaaga atcccaccaa ttccagaaga
    12181 gagaaacgga gtttcagaca gtttcttatc agtgagactc agagttctat cgttctgatt
    12241 ggtgataaga tcatggtgag cagaaagaga tggatggtaa gaaatgtgga tttgatcatc
    12301 gaaagagtct gtgatggtga gcgtgtgttg gtggagagta atttctaggt tttcgacttc
    12361 cttgccaaag gttagctttt ccgtacggct atcatagaca ggttctttgg acatggaaag
    12421 taggctctta atcccgtcag attggatacc tacaaaaagc agaataaagc cgataacggt
    12481 agtcaccaca ccaaagatga gaaatccttt tgtccattta cgcatgctga ttacctctct
    12541 ttcctttttt aagaacaaat tgtaccagac gaacaatgag tagaccgaag aagcgagcta
    12601 cataggaaat gccaagtaaa actagcgaag aagcaccgat agccagtaaa ccagaaccaa
    12661 aaatcaagat aaaggctgat ttggcttggg cgaggacagt gaaactttca actaaaaata
    12721 ggaatccgcc gatgataccc agtatggaaa ctgcaaagaa agccagaatg acagtcaaag
    12781 cggctacaag gattgcgaac agggtcacga ggatggcgat tcccagagga atgccgatag
    12841 gtgctgcaag gagggctaac aaggcgatat gtaaaatttg tcggttattt ttttgagcgg
    12901 gtgcttcatt gattttttta tcgagaagat tggatagaac ttcgtgggcc acttctttgg
    12961 gagttcccaa actagcgatg agttcttctt ctccttcgac tccagcatcg tcaaagagct
    13021 ctctgaaata gtccatggct tcgatacggt cagcttcagg tagtttcttg agatagagtt
    13081 ctagctgagt caggtattca gttcttgtca tggcggatac tcccttctat gatgccattg
    13141 atggtgtctg tatagagtgt ccattcatct tttagggtca agagctgctc tataccaccg
    13201 tttgtcaagg agtagtattt gcgcatgcga ccttggaact ctctagaata ggttgtcaga
    13261 aagctattgc cttccaattt tttgagaatg ggatagagtg tggattcttt gatattagcg
    13321 atcagcttaa tggtttggct aatctcataa ccataagaat caccctgctc cagtacagcc
    13381 aagatgagaa attcaatcaa ggcagaggat gttggaaagt acatgggaaa cctccttttc
    13441 taatgtgtaa gatttttata tataattttt ctacacatac attgtacatc taaaagaaag
    13501 ccctgtcaag agaaatgtgt aaaattttta tatataaaaa acttctagct aaaactagaa
    13561 gtttaaagga tcttatccgc tctgtccact gtaaagaggg ccacagtcat caggatatcg
    13621 atgagcaaga gggcagctac agctggtacc caagagtgga ataggtcaaa actgtaacca
    13681 aagagggttg gcccaaaggc tgctaggata tagcctcctg tttgagatag gccggacaat
    13741 tgggctgtct tttcaggggc gcttgtcttg agtgaaaagt tgaccatgag ataagggaag
    13801 agggcgctgg ttgcggttcc gatgaggaga tggatggcaa gccagtaaat gaaattattg
    13861 attgggaaaa agagcatgga aatgccgacc acaccagcta gtgaaaccag agtgagcatg
    13921 agctgacggt tgcgagtaga taaactggtt gtcaggcttg ggatggtcat tgaaaaagga
    13981 atgctaatca gagataagat agaagtcagc aagccagctt cgtgactgga tagacctgca
    14041 tggatagaca tggtaggtaa ccaggtcatg gcggtgtaaa agatcaagga ttgaaaacct
    14101 gaaaagataa taattgccca aacctgttta ttacgcatga cctttatttg acttttttgt
    14161 ttggtttgtg gagctagtct atgattatag cggtgatttg ggagccagac caaaaaagtt
    14221 gctagacaga gtaacgtgag gaggaggata agtcctttcc aagaactggc ttgtgtaatg
    14281 ggcacagcta gataggaagc cagagccgtt gcaatcccca tagacgttac atataaggtg
    14341 gtcagaaaac caattttctt tggttgattg gcttggataa gactaggaag caggacattg
    14401 atgactgcga cacttgcccc aaccatcaag gttcctagat agagcagggg cagattgatt
    14461 agtcgaatga gtgagccgat ggtcaagaag aagaggctgt aggtgaagag atgctccaat
    14521 ccgattttct gagccagttg ggtagaaaat ggtgaaaaga gggtaaacat aaggagaggc
    14581 aggctggtca agacaccaag cgaactaact tctacctcca gaccttgcga aatatttccc
    14641 aaaataatgg gtaaaacagt gaaaggagtt cgcaaggaaa caccaatcag gataatacct
    14701 ggaacaaaaa agagtgattg ctttttcata taatacctct tctagctgat tttaataaca
    14761 gcttatttta aggctttttc actataatgt caagtaaggt ttctggcttt caagataaaa
    14821 atgggaaagt cttgaaaatt atgatagaat ggtggaagga aaaattcagg agagtagtag
    14881 tgactcaaaa tgttgaaagt cttctcgtat ccattgtaat cagtgcatac aatgaagaaa
    14941 aatatctgcc tggtctaatt gaagacttaa aaaatcaaac ttatcctaaa gagaatattg
    15001 aaattctatt tataaatgct atgtccacag atgggaccac agctatcatt cagcaattta
    15061 taaaggaaga tacagagttt aactcaatta gattgtataa caatcctaag aaaaatcaag
    15121 ctagtggttt taacctggga gttaaacatt ctgtagggga ccttatttta aaaattgatg
    15181 ctcattcaaa agttactgag acttttgtaa tgaacaatgt ggctattatt caacaaggtg
    15241 aatttgtctg tggggggcct agaccgacga ttgtcgaagg aaaaggaaaa tgggcagaga
    15301 ccttgcatct tgttgaggaa aatatgtttg gcagtagcat tgccaattat cgaaatagtt
    15361 ccgaggatag atatgtttct tctatttttc atgggatgta taaacgagag gttttccaga
    15421 aggttggttt agtaaatgag caacttggcc gaactgaaga taatgatatt cattatagaa
    15481 ttcgagaaca tggttataaa atccgctata gcccaagtat tctatcttat cagtatattc
    15541 gaccaacatt caagaaaatg ctgcatcaaa agtattcaaa tggtttgtgg attggcttga
    15601 caagtcatgt tcagtttaag tgtttatcat tatttcacta tgttccttgt ttatttgttt
    15661 tgagtcttgt gtttagtcta gcattgttac cgatcacatt cgtattcata actttactat
    15721 taggtgccta ttttctactt ttgtcattac tcactttgct gactttatta aaacataaaa
    15781 atggatttct aattgtgatg ccctttcttt tattttccat tcactttgct tatggccttg
    15841 ggacgattgt aggtttaatt agaggattta aatggaagaa ggagtacaag agaacaataa
    15901 tttatttgga taaaataagc caaataaata aaaatatgct ataataacaa tatagtaaaa
    15961 ctcttttaag gaggagtaaa tttctatgaa taaaaaactc acagattatg tgattgatct
    16021 ggtggaaatt ttaaataaac aacaaaaaca ggttttctgg ggaatatttg atattttgag
    16081 tatggtggtt tccatcattg tatcttatat cttattctac ggcttgatta atccagcacc
    16141 tgttgactac atcatctata cgagtttggc cttcctgttc tatcaattga tgattggatt
    16201 ttggggattg aacgctagta tcagtcgtta cagcaagatt acggatttca tgaaaatctt
    16261 ttttggtgtg actgccagca gtgtcttgtc atatagtatc tgctatgcct tcttgccact
    16321 cttttcaatc cgtttcatca ttctctttat cttgttgagt accttcttga ttttgttgcc
    16381 acggattact tggcagttaa tctactctag acgcaaaaaa ggaagtggtg atggagagca
    16441 ccgtcggact ttcttgattg gtgctggtga tggtggggcc ctctttatgg atagctacca
    16501 acatccaacc agtgatttgg aacttgttgg cattttggat aaggatgcta agaaaaaggg
    16561 acaaaaactt ggtgggattc ctgttttggg ttcatatgat gatctgccgg aattagccaa
    16621 acgccaccaa atcgagcgtg ttatcgttgc gattccgtcg cttgacccgt cagaatatga
    16681 acgtatctta cagatgtgta ataagctggg tgtcaaatgt tacaagatgc ctaaggttga
    16741 aaccgttgtt cagggacttc accaagcagg tactgggttc caaaaaattg atattacgga
    16801 ccttttgggt cgtcaggaaa ttcgtcttga cgaatcgcgt ctgggcgcag aactgacagg
    16861 taagacaatc ttggttacag gtgctggagg ttcaatcggt tctgaaatct gtcgccaagt
    16921 tagtcgcttc aatcctgaac gcatcgtctt gcttggtcat ggggaaaact caatctacct
    16981 tgtttatcat gaattgattc gtaagttcca agggattgat tatgtacctg tgattgcgga
    17041 tattcaagat tatgaccgtc tcttgcaagt ctttgagcag tacaagccag ctattgttta
    17101 tcatgcggct gcccacaagc acgttcctat gatggagcgc aatccaaaag aagccttcaa
    17161 aaacaatatt cgtggaactt acaatgttgc taaggctgtt gatgaagcca aagtacctaa
    17221 gatggttatg atttcgacag ataaggcggt taatccacca aatgttatgg gagcaaccaa
    17281 gcgcgtggct gagttgattg tcactggctt taaccaacgt agccaatcga cctactgtgc
    17341 ggttcgtttt gggaatgttc ttggtagccg tggtagtgtg attccagtct ttgaacgtca
    17401 gattgctgaa ggtgggcctg taacggtgac agacttccgc atgacccgtt actttatgac
    17461 tattccagaa gctagccgtc tggttatcca tgctggtgct tatgccaagg atggagaagt
    17521 ctttatcctt gatatgggca aaccagtcaa aatttatgac ttggccaaga aaatggtcct
    17581 tctaagtgga cacaccgaaa gcgaaattcc aattgttgaa gttggaatcc gcccaggcga
    17641 aaaactttac gaagaactct tggtatcaac cgaacttgtt gacaatcagg ttatggataa
    17701 gattttcgtt ggtaaggtta atgtcatgcc tctagaagcc atcgatcaaa agattgaaga
    17761 gttccgcact ctcagtggag atgagttgaa gcaagccatt attgcctttg ccaatcaaac
    17821 aacccatgtt gaggcggtaa gtttgctagt caaggagtaa aacgacgaag attagcattt
    17881 acttccgccc atgcgatagc tgtccgtgat tgacaagtgc tagcacgcag acagaacgga
    17941 gatagcgaac cgctgagtgt gtcgctctgc tcgtaaaagc ttagaaacct ttgaacgaaa
    18001 gggataatga aagccttgat tgcaaggctt tttgctttat ggtgggtaag tatcagagtg
    18061 agaaaatttt tggaatgagt agaagtgata gctagaaatt atcagtttct atttccattt
    18121 accctgtggg tacgtgtttg tttccattga caaggagttt gtgggaatag aaatgtaccc
    18181 accttgtttg aatcaagtga agtgtagttg aaggaaatct gttgaaagca atacttcatt
    18241 ttaccgaata agtaataatt taggcaactt caaatcgatt aaaaaaaact attttaaagg
    18301 ttaagagtag acaaaaattg tccactcttt tttgcaaact caatttatca ataaatgaaa
    18361 tgagggaatg taaaatgaaa tattttgagg ttgagttaga aaatcctgat gaatttttaa
    18421 aactacaaac agaagatttt gtgaaagcta atcgcttgct actaaggaag ataatccaga
    18481 gcgttacagt ctatgaagaa aacttcgtca tatcctttaa atctggcatc gaattggaag
    18541 tatgagtctc attccataac ttttatattg aacatatcat ctttttgtgt tatactataa
    18601 attgatataa acaaagatgt aggaggaacc gaaactatga cagcctcaat gcgtttaaga
    18661 taagctggca ataaaaaaag cagaatctat acccgatgat aggctttttt gttgtgctta
    18721 tttatacgat attgagcatt cattagttac ggtgaggata ttggttattt aactatacct
    18781 ttatttaact atgtctttaa tatgaatgtt tccaaattgt atgtatgcag accaaaagcc
    18841 acattgtggg gtttggcctg cattttttat tgcctagaat gctattcaaa atagaaattc
    18901 aagcaaaata atatgcagga gataatataa atggaaaaat acaacaattg gaaacgaaaa
    18961 ttttatgcaa tatgggcagg gcaagcagta tcattaatca ctagtgccat cctgcaaatg
    19021 gcgattattt tttaccttac agaaaaaaca ggatctgcga tggtcttgtc tatggcttca
    19081 ttagtaggtt ttttacccta tgcgattttg ggacctgcca ttggtgtgct agtggatcgt
    19141 catgatagga agaagataat gattggtgcc gatttaatta tcgcagcagc tggtgcagtg
    19201 cttgctattg ttgcattctg tatggagcta cctgtctgga tgattatgat agtattgttt
    19261 atccgtagca ttggaacagc ttttcatacc ccagcactca atgcggttac accactttta
    19321 gtaccagaag aacagctaac gaaatgcgca ggctatagtc agtctttgca gtctataagc
    19381 tatattgtta gtccggcagt tgcagcactc ttatactccg tttgggattt aaatgctatt
    19441 attgccatcg acgtattggg tgctgtgatt gcatctatta cggtagcaat tgtacgtata
    19501 cctaagctgg gtaatcaagt gcaaagttta gaaccaaatt tcataaggga gatgaaagaa
    19561 ggagttgtgg ttctgagaca aaacaaagga ttgtttgcct tattactctt aggaacacta
    19621 tatacttttg tttatatgcc aatcaatgca ctatttcctt taataagcat ggaacacttt
    19681 aatggaacgc ctgtgcatat ttctattacg gaaatttcct ttgcatttgg gatgctagca
    19741 ggaggcttat tattaggaag attagggggc ttcgaaaagc atgtattact aataacaagt
    19801 tcatttttta taatggggac cagtttagcc gtttcgggaa tacttcctcc aaatggattt
    19861 gtaatattcg tagtttgctg tgcaataatg gggctttcgg tgccatttta tagcggtgtg
    19921 caaacagctc tttttcagga gaaaattaag cctgaatatt taggacgtgt attttctttg
    19981 atcggaagta tcatgtcact tgctatgcca attgggttaa ttctttctgg attctttgct
    20041 gataaaatcg gtgtaaatca ttggttttta ctatcaggta ttttaattat tggcattgct
    20101 atagtttgcc aaatgataac tgaggttaga aaattagatt taaaataaac aatattggag
    20161 gaatatttat gtatcttatt ttcatgtaac tcttcctgct aaaatcgcag ggttttccct
    20221 gcatacaagc aaatgaaagc atgcgattat agacaggagg aaatgttatg gaattaatat
    20281 taaaagcaaa agacattcgt gtggaattca aaggacgcga tgttttagat ataaatgaat
    20341 tagaagtata tgattatgac cgtattggtt tagtaggagc aaatggtgct ggaaaaagca
    20401 ctttactcag ggtactttta ggagaattaa ctcccccagg atgtaaaatg aatcgtctgg
    20461 gtgaacttgc ctatattccc cagttggacg aagtaactct gcaggaggaa aaagattttg
    20521 cacttgtagg caagctaggt gttgagcaat taaatataca gactatgagc ggtggtgaag
    20581 aaacaaggct taaaatagca caggccttat cggcacaggt tcatggtatt ttagcggatg
    20641 aacctacgag ccatttagac cgtgaaggaa ttgattttct aataggacag ctaaaatatt
    20701 ttacaggtgc actgttagtt attagccatg accgctattt tcttgatgaa atagtagata
    20761 aaatatggga actgaaagat ggcaaaatca ctgagtattg gggaaactat tctgattatc
    20821 ttcgtcagaa agaggaagaa cgtaagagcc aagctgcaga atacgaacaa tttattgcgg
    20881 aacgtgcccg attggaaagg gctgcggagg aaaagcgaaa acaggctcgt aaaatagaac
    20941 agaaggcaaa aggttcttca aagaaaaaaa gtactgaaga cggagggcgt ttagctcatc
    21001 aaaaatcaat aggaagtaag gaaaaaaaga tgtataatgc tgctaaaacc ctagagcaca
    21061 ggattgcggc cttaggaaaa gtagaagctc cggaaggcat tcgcagaatt cgtttcaggc
    21121 aaagtaaagc attggagctc cataatccat accctatagt cggtgcagaa attaataaag
    21181 tatttgggga taaggctctg tttgaaaatg catcttttca aattccgtta ggagcaaaag
    21241 tggcgttaac tggtggtaat ggaatcggaa aaacaacttt aatccaaatg atcttaaacc
    21301 atgaagaagg aatttctatt tcgcctaagg caaaaatagg ttactttgca cagaatggtt
    21361 acaagtacaa cagtaatcag aatgttatgg agtttatgca gaaggattgt gactacaata
    21421 tatcagaaat tcgttcagtg ctagcatcta tggggttcaa acagaacgat attggaaaaa
    21481 gtttatctgt tttaagcggt ggagaaatta taaaattgtt gcttgctaaa atgctcatgg
    21541 gtagatataa catcctaata atggatgaac ccagtaactt ccttgacata ccaagtttag
    21601 aggctttgga aatactaatg aaggagtaca ccggaactat cgtgtttatc acccacgata
    21661 aacgattact cgaaaatgta gcagatgtag tttatgaaat tagagataag aaaataaatc
    21721 tgaaacatta aatttaaggt agtcgctggt cagtatagtc tgttctggtt ggcgactcca
    21781 ttgttaaaga gtataaagac tttagatttt atgaatatta aaaataggaa cagtcaattg
    21841 aactgctcct atttttctgc taaatatatt gtagttttct tatatgtata atgatagatt
    21901 agcggattct catctacggt acttacttca aatatgaaga agtgatcgcg gttatctctg
    21961 gacttttcct tattgaggac aaagtaattc ttacgtgaag tcgccattgt ttttaggata
    22021 tcatcagtta ggaaggtcaa tggaatattc atgttagagt agcggtagaa gtcacgttca
    22081 aaatcttggt agctctcgct ataatagtcc atttgtaggt gattacgctg aaactcaagc
    22141 tgattcatag agcacctcct cgacaagttc aatactaata atgtctttta atttcaaatt
    22201 gatgtgacct gttgtagttt ttatcaaaat gaaatctttg gtcagacttg gtattgttcc
    22261 agtgtaggaa acacgcttgt ttttttcaat cacttgaatg cgtgtgcgta gctgcccggc
    22321 gtatacttga ctgaggagta ataatttctt ctctagtgat aagtcagaca tgtacgttac
    22381 tttgtttgta tcatcagaga gtgctgatgc atgttcagat aggaaaaagc ccatccattt
    22441 ttgcatcttt gtatcctggt actctcttgc tgattgaaat ggtaaatatg aacggtcaat
    22501 catatcaaat cctttctatg cagaggcaag ggtattttta tcaaattgaa tcgtaaaacc
    22561 ttgaattccc ccacctgtgt aacattcttt aaagcgattg attacctcag tatagattat
    22621 cacagatgag cttgttggct taatgctaaa tgtaaattcc aatggtaatc ggttttcaga
    22681 tttagcatgt actagtcgta tcgatatttc agttgttttg agttttctct gacgaagttt
    22741 tgaagttgct gtttcaacga ttccatgtaa aaatctttca agcatttcaa tatcattaca
    22801 tcctttgcta cggatttctg aaaattgtac tgtatttttt tcttgtttca ttttaatccc
    22861 tccaatccac ccgcggaatg accaccgata agtttactgc gttcaatatt tctggaacct
    22921 tcagttagga cggttccttt ttgtatggct aaaaaaccaa actgttctct gacaacatca
    22981 atagctgtct gaagtctatt atctttttca atttgttcta catcatcaaa gagtgatagt
    23041 agagtatagc tttcatctac gaagccacta taagatacac caatttgtct cactgcacca
    23101 gaggtatatt ttttcggaat aatacaagta catgactcac cattgttttg gggagatttg
    23161 cgggttcaat tttattctga gcatttatag attttttcat ctcagtccta gaatagccaa
    23221 tatgaataga aacgacagta gtcaatacta gggctactgt tccgttccac agtatcattt
    23281 aaaaatcatt ttcacaccct ttcgtctatt agtatagaag aaagctctca gcacacgttg
    23341 aataaaaaag aaaaacgcat attatcagtt ctgaagaacc ttggtaatat gcgttttgtt
    23401 atgtagaaac ttgctccatt ttcttctgaa atggggttgt cgcccagtct atgttattga
    23461 aaatactcca aaacttctat gggtttgtga gcgatataat caggctgata gtttagtaga
    23521 tctgcttgtt ctccaaatcc ccaagtgacg gccaatttct gaatgcctgt ttcttgagcc
    23581 ccctgcatat caaacttggt atctccgatg atgatggctt gttctggtgc tagttgatgt
    23641 gtatgcaagg cttggcgaat gacatcggcc ttatggggtg cttcagggct ggaaccataa
    23701 atgccatcaa agaaatgaaa gattcccaag ttcttagtca tgtcttgagc ggttggagta
    23761 ttctttgtag tggtgatata aagagggtac ttgctcgata acttctcaag caagtctaca
    23821 atatgaggga agagctgagc ttcatggatg ccttttgcct tgtagtaaga acggtatatc
    23881 tgcacagcct cagaaatttg ttctttaggc aggcaggtcg caaaactatt ttcaagaggt
    23941 ggccccataa aaccacgaat cgttttggca tcagggctag gcacccccag ctccttaaag
    24001 gtatgggtaa aggcattgtg aatcccgata gaactgtcaa cgagggttcc atccagatcg
    24061 aaaaaaatcg ctgtgataga ggtcatggtt tctcctattt gataaactta ttctccgaaa
    24121 atttcttttt ggaggcgacg accagtaggg gtggcagcga gtccaccttc agctgtttca
    24181 cgaaaggcag tcggcatgct tgctcctact tggtacatgg catcgataac ttcatccaca
    24241 ggaattttag attcgatacc tgccaaggcc atgtctgctg cgatgaaggc aaagctagct
    24301 cccatggcat tacgtttaac acagggaact tcaaccaagc cggcaacagg gtcacagatg
    24361 aggcctagca tatttttaat gacaaaggca atggcctgac tggcctgata cggtgttcca
    24421 cctgcagcca gagtcaaggc tgcagcactc atagcagagg ccgaaccaac ctcggcctga
    24481 cagccacctt cagcacctga gatagaggca ttgtttgcga tgactagtcc aaaggcaccg
    24541 gcagcaaaga ggaaatccag ctgttgctcg tggctgaggt ctaatttttc aatagcagct
    24601 gttagaactg agggtagaca gccagcactt cctgcggtcg gagtggcaca aaccaagccc
    24661 attttggcat tgtgttcatt gactgcaatg gcatttcggg cagcagagag aatcgtgtaa
    24721 tctgatagag tttttccgtt tttgatgtaa tgatccaatt tggcagcatc tccacctgtc
    24781 aggccactac gagatttatt ttcattgagg ccaagttgga cagaggcttt catgacttcc
    24841 agattgcgtt ccatgagaag gaagacttct tcacgttcgc gaccggtcaa ttcaaactct
    24901 gttgtaatca tgagttctgc gacatttcct tgaaagtcca gatctgcttg ctcgaccaat
    24961 tctttgatag aataaaacat gcttcctcct atttaaagaa attgacattg tggagatgag
    25021 ggatttttcg aatttcttcg atggcctcat cacagttgcg actgtcaact tcgataatca
    25081 taatagcttt ttcaccagct ttttcacgag taacattcat ctgggcgata ttgataccat
    25141 agcgggaaag ggcctctgtt acgagggcaa tcatacctgg aatatcttga tgaacaatga
    25201 tgatagtcgg tgtattcata ttgagagaga cggcaaagcc attgagttcg gttacctgaa
    25261 tattccctcc cccgatagaa atgccagtca cactgatagt tttatgggca tttttaacgg
    25321 taattttagt ggtattagga tgcggagcat tgctgtcttt ttgaatagtc cagacaatct
    25381 tgataccacg tttgtgggca atctccagac tgtttgggat ttcaggatca tctgtatcca
    25441 tgcccaaaat acctgcaaca agggctaggt ctgtcccgtg accacgatag gttttggcaa
    25501 atgagttaaa aagttggaat tcgacttctg tcggagtatc atcaaaaatg gaagagacaa
    25561 tcttcccaat acgaacagcg ccagcggtat ggctactaga tgggccaatc ataactggtc
    25621 cgatgatatc aaagacagat tgaaaacgaa gtgatttcat cagtttcccc ttataaaaat
    25681 tcttatctct attatatcaa agaatgaggg tcttgacttt aattgcggat gaaaaccttt
    25741 ctaatacctc aaatagcata aaaatagtat cttttatgac aaaaaacaca ttatttaggg
    25801 aaataaaaaa taattttgta atatttctac ataaaagtgt caagaaacgg taatatttaa
    25861 agggtatcat agaattatag aaagaaggag aattttcaaa tatgaaatca acaactaaaa
    25921 agattaaaac aactcttgca ggagtagctg ccttgtttgc agtatttgct ccatcatttg
    25981 tatctgctca agaatcatca acttacactg ttaaagaagg tgatacactt tcagaaatcg
    26041 ctgaaactca caacacaaca gttgaaaaat tggcagaaaa caaccacatt gataacattc
    26101 atttgattta tgttgatcaa gagttggtta tcgatggccc tgtagcgcct gttgcaacac
    26161 cagcgccagc tacttatgcg gcaccagccg ctcaagatga aactgtttca gctccagtag
    26221 taagtgaaac agttgtttca actgtaagcg gatctgaagc agaagccaaa gaatggatcg
    26281 ctcaaaaaga atcaggtggt agctatacag ctacaaatgg acgttatatc ggacgttacc
    26341 aattaacaga ttcatacctg aacggtgact actcagctga aaaccaagaa cgtgtagcag
    26401 atgcctacgt tgcaggacgt tacggttcat ggactgctgc taaaaacttc tggcttaaca
    26461 atggctggta ttaagaacta acgaacaaat aatatgtaaa gagtcgagga aatccctcga
    26521 ctttttttat aatttctttg gttgataatg ggtctctact tcttttttga gatgagacag
    26581 catagaagtt tcctcggtca gctctaggag tgagaaccct ttttggataa gtttcgcatc
    26641 atccctacaa atcccaatac ggacatagca gatagccagc ctatcgtagc ttttcttatt
    26701 cttggcgtcc tccagtaaag tgtagcagat ttgcttacac atgtttttat cttgattgta
    26761 tagatagagt gtggaaaggt ttaatagaat tgtcattcgc aagtcatata aatgttgata
    26821 gtttttatag acttctaagc gttgcaagat tttctcggat tattgacaaa taaacggaca
    26881 ttctgataga cgattgccct agcggcttag tgtaaacttt acaaatttgg cttgtaattt
    26941 ttttataaaa ttcacttcag ttacttgaca gttattatat tttcgcttat gcttattata
    27001 tagataaaag gacgggatag gagtttaaaa atttgaaagc gctatccata ttgtttattg
    27061 gactgattct tgagtaatag ttagagtttg cttaatacta ctctggttgt tcaaggacct
    27121 gatacagaag cgcaccattc tcccggttaa gagtaagttc gttgttaaaa aatgaaatga
    27181 gatagaagga aggtgagtcc aatggactac gatacttgtt gataaattac tatagctgtt
    27241 tttacggaac agctctcgat aataggagaa ataaatcatg agaaaattag ttaaaattgg
    27301 tgttgcatct ttaatggtat gtggtatact tgctactaca aatgctttag cagtatgggt
    27361 tgagggtggt caatggaact atggagtagg ttggacagga acttttggat attctgatta
    27421 cttacattct actcgatatc atacagcaac tgttagacat gggggtagaa cctctaagga
    27481 ttatgcaaaa cctgaggcat gggctagagc ttccctcacc aagattccgc caacaggaat
    27541 ggaatatttc tatggatttg agtaaaattc ttccctctcc tatttggaga gggaaaactg
    27601 tagtggaggc acttttatga aaaaaatcag taatttctgt atgttactcc tgcttctgtg
    27661 taccactttt tttgttttta atgtaaacta tacacgagaa gtggttcgga ttcaagaaat
    27721 gggaaagact gtagattctt tggatttgta tttgaaagat attaacgaac ctgcagcgtc
    27781 tgttcttcga ttttttgagg atgtatcaaa ggagtataaa gtctccatca tcaaaacaga
    27841 cagtggtgat gaggtggtca agtctggtgt ttttgataaa gataccttcc cctaccaaga
    27901 gtttgggatt tcttctcttg attttaccac agatggtgaa ggagtctata gtaataaaga
    27961 aatttccaat aaacttggta cgattccgac ctttctaaaa gccaaaccta ttcagcttat
    28021 gacttttcaa acctatatca aggatacatc tcgtagttta aatggtcgct atacgataac
    28081 ttctacacaa gagatggaca aggataggat tgtacagaaa tggagcgatt ttttcaagat
    28141 agaccaggct accttgctag agccgaccta caaaagtgca gtggaagtca taaatcgaga
    28201 tttgctttta tctgccattg tttttgtctt ggctattttg cttcttgtgt tagtgacagt
    28261 gtatcaaccg atgatggaga tgaaaagagt tggggtacaa aaattacttg gttttcaaga
    28321 tagggctgtt ttagctgatg ttgtaaaagg caacctttac ctcctcctag gtggggctct
    28381 tgtgatcaat ctaggcgtgt ttttcttgct tgattataag ccaaaagatt tgtttcctat
    28441 gctgtggttg tctcattttt tgctgttgca gctttatctc tttatcagtt ggttgactta
    28501 cctcttaatc caaaaaatga caatcagctc tctgctgaaa ggtttttcat ctttcaaatt
    28561 tggtcttatc ttcaattatg tgatgaaaat agggacaact attttactga cggccttact
    28621 gattggggtg ggcagaagtt tagaacaaga aaacaaagaa cttgcttatc agcaacagtg
    28681 ggtaagtcaa ggtaattacc tgaccttaga aaccttcaaa ctcaatgata atctgtggca
    28741 agaagagcta gcagggtcag ggaaatctac agattatttc tatcgatttt atcaggattt
    28801 ggtagaaaaa acgcaggcgg gctatgtgca gagtagcagt cttcctgtaa aaaattttgt
    28861 ccaatcagaa cagattcagc aatatcagtt aacagatacg gtggatgttt actatgccaa
    28921 tcgcaatttt ctaaagagca agggattcaa gctaccaaat accggtatta aaaaagttat
    28981 tttgatgcca gcaagtacga aaggtgaaga agataaaaat cagctcttgg ggaagttaat
    29041 tgcctttcat tcgatgaagt atgaagagca gcaaaaacga acgatagagg agatggatgt
    29101 cgagattgcc tattatgaag gagattggtc atttttccca tatagtgata agcgaaagga
    29161 aaatctctcc aatccaatta ttagcttggt caatgattct gatatgatgt gggatgagaa
    29221 agcctccctg tcaacaactg gcttaaataa tccgattaaa attgaaaata cggttcaaca
    29281 tcaaaaagag attacagagt tagttgagaa attgtcagat ggaaattatt taaaattttc
    29341 atctattcaa gccattcaac aagagaaagt ggattcttat cgagatgctg ttcggaattt
    29401 taacctactc tttgctttgt ttggtctcct tagcatgatg atttcctact tcttactagt
    29461 aacaactttc ttattgaagc gcagggatat cattaccaag aagtttatgg ggtggaaact
    29521 ggtcgatcgc taccgtcctc tcctcgttct gctcttgctg ggctatagtt tccctcttct
    29581 agtcttgatt ttctttgccc atgcgttctt accacttcta ctgtttgcag gttttacatg
    29641 tctggatata ctatttgtgc taggcttagc ttctaggatg gagaaaagaa gtctagtaga
    29701 gttattgaaa gggggcatct tatgattgag ttgaaaaata ttaccaaaac cattggggga
    29761 aaagtgattt tggataactt atctctcagg attgatcagg gggatttggt agctattgtt
    29821 ggtaagagtg gtagtgggaa gtcgaccttg ttaaatttat tgggtttgat agatggtgat
    29881 tatagcggac ggtatgagat ttttggtcag acaaatctag cggttaattc tgctaagtcg
    29941 caaacaataa tccgtgaaca tatctcttat ctgtttcaaa attttgccct gattgatgat
    30001 gaaacggtcg agtacaatct catgctggcg ctgaaatatg tgaaattgcc taagaaagac
    30061 aagctcaaaa aggtggaaga gattttagag agagtaggtt tatcagctac tttgcatcaa
    30121 agggtctccg agttgtctgg gggcgaacaa caacgaattg cagttgctag agccatctta
    30181 aaacccagcc agctgatttt agccgatgaa ccgacaggtt ctctggatcc tgaaaataga
    30241 gatttggtct tgaagtttct cttagagatg aatcgagagg ggaaaacagt cattatcgtg
    30301 acccacgatg cttatgtagc ccaacaatgt catcgaatca ttgaattagg cgagggaaaa
    30361 tgagttcgtt cagcttcatt tgaccgactg aatactcata tttttcagag agaaatagca
    30421 taaataggcc taggaatgat atttttatgt cattttcctg ggtttttctg ttttaaattg
    30481 aaaaaaatta caacttaggc ttgacaaagg atgcatatag atgtattgtt tatacaaata
    30541 aaaggaataa acataaagta ggctttatta tgaataagat gaagaaggtg ttgatgacga
    30601 tgtttggttt agtgatgctc cccctactat ttgcttgtag caacaatcaa tcggctggaa
    30661 ttgaagccat caagtccaaa ggaaaattgg ttgtagccct caatccggat tttgctccat
    30721 ttgaatacca aaaagtggtt gatgggaaaa atcagattgt gggttcagat atcgaattag
    30781 ccaaggctat cgcaacaaaa ctaggtgtcg aattggaact atctcccatg agttttgata
    30841 atgtactggc tagtgttcaa tcaggaaaag ccgaccttgc catatcaggt gtttctaaga
    30901 cagatgaacg gagcaaggtg tttgactttt ccattcccta ctatactgca aaaaataaac
    30961 tcattgtcaa aaaatctgac ttgactactt atcagtctgt aaacgacttg gcgcagaaaa
    31021 aggttggagc gcagaaaggt tcgattcaag agacgatggc gaaagatttg ctacaaaatt
    31081 cttccctcgt atctctgcct aaaaatggga atttaatcac agatttaaaa tcaggacaag
    31141 tggatgccgt tatctttgaa gaacctgttt ccaagggatt tgtggaaaat aatcctgatt
    31201 tagcaatcgc agacctcaat tttgaaaaag agcaagatga ttcctacgcg gtagccatga
    31261 aaaaagatag caagaaattg aaagaggcag tcgataaaac cattcaaaag ttgaaggagt
    31321 ctggggaatt agacaaactc attgaggatg cctttaaagc atccattgaa aaatagaaag
    31381 aaggaaaaga acatgagtaa agaaaaagta attttggcct attctggcgg tttggacaca
    31441 tcagttgcta ttacctggtt aaagaaagac tatgatgttg tttcagtttg tatggatgtg
    31501 ggtgaaggga aagacttaga tttcatccat gataaggctc ttaaggtttg ggcagtagaa
    31561 tcttatgtca ttgatgttaa ggacgaattt gctacagatt atgtgctagt ggctcatcag
    31621 tcacatgcct attatgaaca aaagtatctc ttggtatctg ccttgagccg ccctctgatt
    31681 tctaaaaaat tggttgaaat agcgcatcag ataggagcga ctacaattgc tcatggttgt
    31741 accggtaagg ggaatgacca agtcgaatac cagattgcag tcgctaagaa agcgaatgaa
    31801 gctaaaaagt gatggagtga tgggttcaga ataagaggtt ggtagattat cagcctcttt
    31861 cttgtcgttg aaaaagtgag atatattgac ttttgaaaaa aatgtcataa ttgagttggg
    31921 ataaacgtaa aagtcatact ttctcgttaa atcgttcggc gattctatga aaaggtcgag
    31981 gagctccctc gacttttttt ataatttctt tggttgataa tgggtctcta cttctttttt
    32041 gagatgagac agcatagaag tttcctcggt cagctccaga agggagaacc ctttttggat
    32101 aagtttagaa tcgtctgtac aaatcccaat gcggacatag cagatagcca gcctatcgta
    32161 gcttttctta ttcttggcgt cctccagtaa agtgtagcag atttgcttac acatgttttt
    32221 atcttgattg tatagataga gtgtggaaag gtttagcagg attgctattc gcaagtcata
    32281 taaatgttga tagtttttat agacttctag gcgttgcaat atttttccag tgatgagatg
    32341 gaggtattca atgggaaaac tgaaaagaat ggtattgagg atttttagat cactctcata
    32401 ccatgtatct tgtttttcaa tttttctcca aagttttttg acaacctgtt ctgcgtgatc
    32461 tgatagttcc cctgccccgt gttgacggag ataaatgaca acttcaagca tatctctgat
    32521 ttcttctata gggagatcgt ggtgggtctt gaggtagtct tggcattttt gaaataaatt
    32581 gacaagatcg ctggtcccga taatggaacg catattgaga taggtttgca taatctctgt
    32641 gcgttgactt ggttgataaa gttgacagat atattcaaac tcttcaaaac tcatattgat
    32701 ttgacggaga agaaattcca tattttcata ttttggggtt gtcttgccac tttcgatttt
    32761 tgataagctg gttcttgaga gaacaccccc acagacctct tcttgggtca gcccctttga
    32821 ctcacgtatt tctttataga cctttccgaa atcataacgc atgattttct cctttctgtg
    32881 aaaaaagtta acaaataata aaatcttgtt aaaaatattg tatcatagca gtgtaaaaag
    32941 taaaaaagaa agtaaaaata aatttgtgaa aaaagttaac gtaattttaa aatgagctta
    33001 ataagaggta tgatgtaggt agaaaataaa gataggagaa aaagtatgaa taaaaaaata
    33061 ttttgtattc tagtatgtat gatatcattg attagtctgg ccataatctt tccatggggc
    33121 tggtcgatat aatgtaaggg agcttgagtt gttaaaggag gcttagtcct tggacaaaaa
    33181 tctgctgtac tacaatgctt ggaaagatat caagaacaaa gaatgatacg attgaaaata
    33241 aactgattca agaactactg ggcagtgatt gaggtttggg tgacatcaaa gaaaagttag
    33301 attaattcta aagaattgag tatttctatg ttaaaaaaat atccttgtac gatgcaacat
    33361 gatcagtcag attgtgctgc ggcagttgtt tcaacagttc ttttatctta caaaaaggaa
    33421 ctatcaatca tgaagattcg ggaaattatt ggtacagaca tgtatggaac gactgtcagt
    33481 ggtattgttt caggtctgaa taagttgaat tttacagtaa aagctgttcg agtggcactg
    33541 gaagatttga ctccaaaatt aacatttcct gcgattcttc aagttaagaa tgatttaggt
    33601 caaaatcatt ttgtggtatt acatagtata aaggagaaaa taaatggaac tcgtattacc
    33661 aaataattat gttgctcttg agcaagagga gatgatgtat cttgatgggg gggcttattt
    33721 aagcaagagt gcttgtcaag gaatttgcgc agctttagct atgagcccag gaacttttat
    33781 agcattgact ggagctgcag ttttaacaaa aaaactaata aactatatta aggttggagg
    33841 ccttggaggt tggcttattg gtgcagcagc aggtgtattg gctcttatct ttttgataaa
    33901 aatttgtgta gatactacga taaaggttct attaaacaga atctttaagg caagtaaggt
    33961 aatgaaaagg aggatatttt ctctttttgt actagatttg atggtctata tatttttagg
    34021 ttatgtttta gtgatacaaa aagacgtata tctgttttca attctgataa ttttttctaa
//
LOCUS       DAVHEX010000016        33948 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVHEX010000016 DAVHEX010000000
VERSION     DAVHEX010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33948
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.016"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(229..591)
                     /locus_tag="VVL45_000758"
     CDS             complement(229..591)
                     /locus_tag="VVL45_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEU4186961.1"
                     /translation="MLEVGLNSLISLFTFTFDILYPIVKVGHTDDYSHGAVKLSVSLV
                     DKDAMKKISVTVIGYFEINIDENITDILYVNGTAILYPYLRSIVSIVSAIDSSEAMLL
                     PTINVLELLDKSQPFEEE"
     gene            599..679
                     /locus_tag="VVL45_000759"
                     /pseudo
     CDS             599..679
                     /locus_tag="VVL45_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(1388..2077)
                     /gene="rplA"
                     /locus_tag="VVL45_000760"
     CDS             complement(1388..2077)
                     /gene="rplA"
                     /locus_tag="VVL45_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEU4186962.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            complement(2285..2710)
                     /gene="rplK"
                     /locus_tag="VVL45_000761"
     CDS             complement(2285..2710)
                     /gene="rplK"
                     /locus_tag="VVL45_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEU4186963.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            2842..2979
                     /locus_tag="VVL45_000762"
     CDS             2842..2979
                     /locus_tag="VVL45_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4186964.1"
                     /translation="MKIKEQTRKLAAGCSKYSFEVADKTDEVSSKHCFEVVNRTDVVS
                     R"
     gene            complement(3019..3735)
                     /locus_tag="VVL45_000763"
     CDS             complement(3019..3735)
                     /locus_tag="VVL45_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEU4186965.1"
                     /translation="MKKRYLVLTALLALSLAACSQEKTKNEDGETKTEQTAKADGTVG
                     SKSQGAAQKKAEVVNKGDYYSIQGKYDEIIVANKHYPLSKDYNPGENPTAKAELVKLI
                     KAMQEAGFPISDHYSGFRSYETQTKLYQDYVNQDGKEAADRYSARPGYSEHQTGLAFD
                     VIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AESGLSLEEYYGFEGGDYVD"
     gene            complement(3738..4208)
                     /locus_tag="VVL45_000764"
     CDS             complement(3738..4208)
                     /locus_tag="VVL45_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU4186966.1"
                     /translation="MCLICQRIDLIKKEENPYFVKELETGYLVVGDHQYFEGYSLFLA
                     KEHVSELHHLKKETRLRFLEEMSLVQEAVAKAFAAEKMNIELLGNGDAHLHWHLFPRR
                     TGDMNGHGLKGRGPVWWVPFEEMTAETCQAKPDEIKRLVKRLSSEVDKLLEIKE"
     gene            complement(4210..5247)
                     /locus_tag="VVL45_000765"
     CDS             complement(4210..5247)
                     /locus_tag="VVL45_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEU4186967.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREIADYLVKTLEGFGYQPVRTSK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKMDRIGGFPWNMIEGENCTI
                     HVASTGEKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDAKVTSEKETRALGIEVG
                     DFISFDPRTVVTETGFIKSRHLDDKVSAAILLNLLRIYKEEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVIATERM
                     VDAYLKSTLVD"
     gene            complement(5452..6777)
                     /gene="brnQ"
                     /locus_tag="VVL45_000766"
     CDS             complement(5452..6777)
                     /gene="brnQ"
                     /locus_tag="VVL45_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEU4186968.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIANVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            complement(6948..7040)
                     /locus_tag="VVL45_000767"
     CDS             complement(6948..7040)
                     /locus_tag="VVL45_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU4186969.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            complement(7083..7235)
                     /locus_tag="VVL45_000768"
     CDS             complement(7083..7235)
                     /locus_tag="VVL45_000768"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU4186970.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLEGAHNLPLSQ
                     LADSYD"
     gene            complement(7395..7973)
                     /locus_tag="VVL45_000769"
     CDS             complement(7395..7973)
                     /locus_tag="VVL45_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEU4186971.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            complement(8027..9427)
                     /gene="pepV"
                     /locus_tag="VVL45_000770"
     CDS             complement(8027..9427)
                     /gene="pepV"
                     /locus_tag="VVL45_000770"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEU4186972.1"
                     /translation="MIAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGAARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVASVSLSEHGHTPHYVPMEDPLV
                     QTLLNVYEKQTGLQGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            complement(9440..10045)
                     /locus_tag="VVL45_000771"
     CDS             complement(9440..10045)
                     /locus_tag="VVL45_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEU4186973.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYNEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDDKLEPSYRLPVDEIIEKR"
     gene            10216..11016
                     /locus_tag="VVL45_000772"
     CDS             10216..11016
                     /locus_tag="VVL45_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU4186974.1"
                     /translation="MKKFSLLLAILPFLVACGNQVTPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            complement(11200..12033)
                     /locus_tag="VVL45_000773"
                     /pseudo
     CDS             complement(11200..12033)
                     /locus_tag="VVL45_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
     gene            complement(12023..13870)
                     /gene="uvrC"
                     /locus_tag="VVL45_000774"
     CDS             complement(12023..13870)
                     /gene="uvrC"
                     /locus_tag="VVL45_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEU4186975.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDEIYFKSMAQEVSDFLKGQDNKIIDELKGKMAAAAQTMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDSKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGKPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMRHFKSLTKIKEA
                     SVDEIVEVGVPRAVAEAVQTKLNPQETEILLQVAEERVDYQTEGNHNKP"
     gene            complement(13910..14611)
                     /locus_tag="VVL45_000775"
     CDS             complement(13910..14611)
                     /locus_tag="VVL45_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEU4186976.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            complement(14690..15922)
                     /gene="murN"
                     /locus_tag="VVL45_000776"
     CDS             complement(14690..15922)
                     /gene="murN"
                     /locus_tag="VVL45_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HEU4186977.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSDYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            complement(15927..17147)
                     /gene="murM"
                     /locus_tag="VVL45_000777"
     CDS             complement(15927..17147)
                     /gene="murM"
                     /locus_tag="VVL45_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HEU4186978.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRVV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            complement(17259..18038)
                     /locus_tag="VVL45_000778"
     CDS             complement(17259..18038)
                     /locus_tag="VVL45_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEU4186979.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALTTNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIRDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            complement(18107..19768)
                     /locus_tag="VVL45_000779"
     CDS             complement(18107..19768)
                     /locus_tag="VVL45_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU4186980.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPERIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEEYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            complement(19943..22165)
                     /gene="recJ"
                     /locus_tag="VVL45_000780"
     CDS             complement(19943..22165)
                     /gene="recJ"
                     /locus_tag="VVL45_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEU4186981.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPYRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKAYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            22442..23200
                     /locus_tag="VVL45_000781"
     CDS             22442..23200
                     /locus_tag="VVL45_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU4186982.1"
                     /translation="MALVEFKNVEKYYGDYHALRNINLRFEKGQVVVLLGPSGSGKST
                     LIRTINGLEAVDKGSLLVNGHQVAGASQKDLVPLRKEVGMVFQHFNLYPHKTVLENVT
                     LAPIKVLGIDKKEAEKTAQKYLEFVNMWDKKDSYPAILSGGQKQRIAIARGLAMHPEL
                     LLFDEPTSALDPETIGDVLAVMQKLAHDGMNMIIVTHEMGFAREVADRIIFMADGEVL
                     VDTTDVDNFFDNPSKPRAQQFLSKIINHESDKVK"
     gene            23239..24003
                     /locus_tag="VVL45_000782"
     CDS             23239..24003
                     /locus_tag="VVL45_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU4186983.1"
                     /translation="MISLFGLAAAKPVQADTSIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITELGKKKGLKFK
                     FVELGSYPELITSLHAHRIDTFSVDRSILSGYTSKRTALLDDSFKPSDYGIVTKKSNT
                     ELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            24016..24693
                     /locus_tag="VVL45_000783"
     CDS             24016..24693
                     /locus_tag="VVL45_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU4186984.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAVGSFILAMVLGIFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLPLISDHIIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLIILPQAFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            24703..25362
                     /locus_tag="VVL45_000784"
     CDS             24703..25362
                     /locus_tag="VVL45_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU4186985.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVT
                     RNGKNPVLRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNAVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLYILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            25620..26810
                     /locus_tag="VVL45_000785"
     CDS             25620..26810
                     /locus_tag="VVL45_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEU4186986.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHSLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKTSGDHTKNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(27131..28012)
                     /locus_tag="VVL45_000786"
     CDS             complement(27131..28012)
                     /locus_tag="VVL45_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEU4186987.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(28153..29481)
                     /gene="vncS"
                     /locus_tag="VVL45_000787"
     CDS             complement(28153..29481)
                     /gene="vncS"
                     /locus_tag="VVL45_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HEU4186988.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENSLTFFIDFPKVVQD"
     gene            complement(29478..30134)
                     /gene="vncR"
                     /locus_tag="VVL45_000788"
     CDS             complement(29478..30134)
                     /gene="vncR"
                     /locus_tag="VVL45_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HEU4186989.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(30183..30266)
                     /gene="pep27"
                     /locus_tag="VVL45_000789"
     CDS             complement(30183..30266)
                     /gene="pep27"
                     /locus_tag="VVL45_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198523890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HEU4186990.1"
                     /translation="MRKEFHNVLSSDQLLTDKTPARDYNRK"
     gene            complement(30446..31825)
                     /gene="vex3"
                     /locus_tag="VVL45_000790"
     CDS             complement(30446..31825)
                     /gene="vex3"
                     /locus_tag="VVL45_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HEU4186991.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(31877..32524)
                     /gene="vex2"
                     /locus_tag="VVL45_000791"
     CDS             complement(31877..32524)
                     /gene="vex2"
                     /locus_tag="VVL45_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HEU4186992.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQINYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKDTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(32537..33814)
                     /locus_tag="VVL45_000792"
     CDS             complement(32537..33814)
                     /locus_tag="VVL45_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4186993.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNAITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
BASE COUNT         9631 a         7718 c         6334 g        10265 t
ORIGIN      
        1 tatacataaa ctaacctcct cattattagc aaggcttcta ctgattataa cctaattctt
       61 agactacttc aagactaact gtccaataat aatctacaga taaatcatcc actcttaatc
      121 aaattttatt caatccatct actcattccc aaaaggttgc tgtataatgc aagacatttt
      181 tcttactgat tttacttttc cttaccctca atttagtgag ctaataaatt attcttcttc
      241 aaaaggttga gatttatcta gtaactctaa aacattaatg gtaggtagca acattgcttc
      301 actgctatca attgccgaaa ctattgaaac aatagaacgt aaataaggat aaagaatagc
      361 agtaccattt acgtatagaa tatcggtgat attttcatct atatttattt caaaatatcc
      421 tattacagtt acagatattt ttttcatggc atctttgtca actaaagaaa cactaagttt
      481 tacggctcca tgactataat catctgtatg accaacctta actatgggat ataatatatc
      541 gaaggtaaat gtgaacaaac tgattaggga atttaaacca acttctaaca atgtgttata
      601 ctcaatgaaa accaaagagc agactaggaa actagccaca ggttgctcaa aacactgttt
      661 tgaggttgta gatggaactg acgtagtcag ctcaaaacac tgttttgagg ttgtggatag
      721 aactgacgta gtcagtaacc atacgcacgg caaggcgacg ttgacgtggt ttgaagagat
      781 tttcaaagag tattagaaag ctcgatggac tattccagat tcattccact agatttcgaa
      841 atcgagtagg ggataatctc tagcccctct cacaccaccg tacgtgccgt tcggcatacg
      901 gcggttcaac taacttttaa cgcatgtcgt tcaaggtaat aatccaaaca cgaaacctgt
      961 tcacgttttt caaggactgg ttttgatata gcacgtttaa gtaccgactt ctgagctact
     1021 atagtagatt gaaactagaa tagtacatct cttcttctaa aacattgtta gaaatcgatt
     1081 tggctgccct gatcgattcg tcctactctt atttcatttt actataattg atagtggtcg
     1141 ccccagccag ataccttatc cgctatatag gccttattat cacgttcctg ttcgtagagc
     1201 cacgatttcg ctatcccttc ctctgacgat tcttatttcc agcgtcagac ttgggaatcg
     1261 ctattgggtt caccggtagt aggtgcccac cgaggactta cacctcagat atacgacatg
     1321 cccgtcgtac atcaaaaaaa gttgagacat agtgtcttca acttttttac attagtttaa
     1381 ctactgatta aagtgagttt acgtcaactt tgataccaac accttgagta gttgtgatag
     1441 tcaagtttgt tacgtaagtt cctttagctg tagctggttt tgctttttgg attgtttcgt
     1501 tgaaagcttt aaagttttca accaattttt cagcttcaaa tgatacttta ccgatgattg
     1561 cttgaacgtt acctgcacgg tcagcacggt aagtgatttt accaccttta gactcttcaa
     1621 ctgctttagc aacatccatt gttacagtac cagttttagg gtttggcatc aagttacgtg
     1681 gtccaaggac acgtccaaga cgtccaacaa gagccatcat atcaggtgta gcgataacta
     1741 cgtcgaagtc caaccaaccg tcgttgattt tagcaacaag gtcatcttca ccaacaaagt
     1801 ctgcaccagc agcttttgct tcttcagctt ttgcaccacg tgcgaaaaca agaacacgtg
     1861 aagttttacc agtaccgttt ggcaatacca ttgctccacg gatttgttgg tcagcttttt
     1921 taacgtcgat gttcaagttg taagcaactt ctacagttgc atcaaatttt gcaaagttag
     1981 tttcttttgc aagtgctaca gcttcttcta cactgtatgc ttttgtgctg tcgattttct
     2041 caagagcagc acgaagttgt ttgctttttt tagccatttt ctattctcct tgtaagtggt
     2101 tcaatcgatt ttcatctccc acgtcacttc tcgaaatgat gaagtcttgc gggttttcag
     2161 tctactattt ttctcttcct acggccttaa gctcccttgc tgtacccaag tacaagcctc
     2221 agtcacttgc ctagtatgaa aagcaactaa tagactgctg tgagattttc tactgtgtta
     2281 ttgattagtc aacaacagtg aatcccatag aacgagcagt accttcgatc atacgcattg
     2341 cagactctac gtttgctgcg ttcaaatctg gcatcttagt ttctgcaatt tcttgtactt
     2401 gtgcacgagt aactgtagca actttagttt tattaggtgt acctgatcct ttttcaacac
     2461 ctgcagcttt tttcaaaaga acagcagctg gtggtgtttt cgtaacgaaa gtaaatgatt
     2521 tatcttcgta aactgagata acaactggaa tgatcatacc agcttggtca gctgtacgag
     2581 cgttgaactc ttttgtgaat cccatgatgt tgataccagc ttgaccaaga gcaggtccaa
     2641 ccggtggagc tggtgtagct ttaccagcag ggatttgcaa ttttacaagt ttttcgactt
     2701 ttttagccat ttttaaatcc tcctttgtgg ttttggcggt aattagagat ttttacctcc
     2761 cacaagtatg tttttcacat acccatccat tatacactat ttatctggaa ttgcaagaga
     2821 aaagtttatt ttttgtactc aatgaaaatc aaagagcaaa ctaggaagct agccgcaggc
     2881 tgctcaaagt acagctttga ggttgcagat aaaactgacg aagtcagctc aaaacactgt
     2941 tttgaggttg tgaatagaac cgacgtagtc agtaggtaag gcgacgctga cgtggtttga
     3001 agagattttc gaagagtatt aatcgacgta gtctccgcct tcaaagccat agtattcttc
     3061 caaactgaga ccactctcag caatttcttt tgcttctttt ccgacataac gcaggtgcca
     3121 ttcctcagcc atatagcctg tttctttttc cttacctttg agataacgga caacaaagcc
     3181 ataatcagct gcatggtcca aaagccattg ggctgctttt tcttctgtta ccaaatcacc
     3241 atcagtccca atcacatcaa aggccaagcc tgtctggtgt tcgctataac caggtcgagc
     3301 agaataacgg tcggcagctt ctttcccatc ttgattaaca taatcttgat agagtttggt
     3361 ctgagtttca taacttctaa aaccactgta atgatcgcta atggggaaac ctgcctcttg
     3421 catggctttg atgagtttga ccaactctgc cttggctgtt ggattttccc ctggattata
     3481 gtctttagac aatggatagt gtttgttggc tacgatgatt tcatcgtatt tcccttgaat
     3541 gctgtagtaa tcacctttat tgaccacttc tgctttcttc tgggcagctc cttgagactt
     3601 actaccgact gttccatcag ctttggctgt ctgttctgtc ttagtttctc catcttcatt
     3661 ttttgttttt tcttgtgaac aagctgctag actcaaggct agcaaagctg tcaagactag
     3721 gtatcttttc ttcatttcta ctcctttatt tctaatagtt tatctacttc tgacgataaa
     3781 cgtttgacta atcttttaat ctcatccggt tttgcttggc aggtttctgc tgtcatttct
     3841 tcaaagggaa cccaccagac tggtccacga cccttgagac catgaccatt catatcacct
     3901 gtccgtcgtg gaaacagatg ccaatgaaga tgagcatcgc catttcctag cagttcgata
     3961 ttcattttct cagcagcaaa ggccttggca actgcctctt ggactaaact catttcttct
     4021 agaaaacgga gtcttgtctc ctttttcaaa tggtgcaatt cgctgacatg ctccttggct
     4081 agaaagagac tatagccttc aaaatactgg tggtctccaa ccacaagata gcctgtttcc
     4141 aactctttga caaagtaagg attttcttcc ttcttgatga ggtcaattct ctgacaaata
     4201 aggcacatat tagtccacca acgtgctctt aagataagca tcgaccattc gttctgttgc
     4261 gatcaccgag tcaatatggg tacgctcata ggaatggcta gactctatac cagcaccgag
     4321 aagggcgtgt ttgacttctg cccctgcaga catagccgct gaagcgtccg aaccataaaa
     4381 tggatagata tccagcttaa atggaatatc ttgctctttc gccaaagcca ccaaatgttg
     4441 acggaagtca tagtgataag gtccagaagc atccttgaca cagatagaca ctgtatattc
     4501 gtctgtttgc tggtcatctc ccatggctcc catatccaca gccagatatt ctactacctg
     4561 agcaggaatg ttagagtttg caccgtgtcc cacttcttca aagactgaaa aagcaaaatg
     4621 agttgttacg ggcaattcaa tcttctcttc cttataaatg cgaaggagat tgagcaaaat
     4681 cgccgcactg accttgtcat ccaaatggcg agacttgata aaacctgtct ctgtcacgac
     4741 agttcgtggg tcaaaactga taaaatcacc gacctcaatg ccaagagcac gagtttcttt
     4801 ttcactagtt actttggcgt ccaaacgcac ttccatattg tcttgcgtgc gttctgcagt
     4861 tcctgcatcc ttatagacat ggcaagaagt ttggtggatg aggatggttc ctgatacttt
     4921 ttcacctgtg ctagccacat gaatggtaca gttttctcct tcaatcatgt tccaaggaaa
     4981 gccaccgata cggtccattt tgagacggcc gtctggtttg acagcacgga caatagcacc
     5041 aagcgtatct acatgggcag tcacatagcg atgttgctca tcattttgac ctttaatagt
     5101 tacattgaca ccgcccttgg atgtgcgaac cggctggtaa ccaaaacctt ctagagtctt
     5161 gactaaatag tccgcaatct cacgagtaaa gcctgttggc gacgcaatgg ctgtcagttc
     5221 tttgatatat tctactgttt gattcatttc ttcacctctt ttgctaccta ttataacaca
     5281 tttatcatcg gataaaaaaa gaaaatcact cctgtagact tgactacaaa aagtgatttt
     5341 atactcaatg aaaatcaaag agcaaactag gaaactagcc gcaggctgta cttgagtacg
     5401 acaaggcgac gttgacgcgg tttgaatttg attttcgaag agtattagtg attattccat
     5461 ttcaaaaaca tcactttctt gcttgtttgg tagaaccaat gagagcaaga ttccaacaat
     5521 ggctggcacc aaccatggga gagatgcctt ggcaaaagga agaacgctaa caagatttgc
     5581 aagaaactca accttaaacg agcttcctag tacgtttgca atggcaataa ctgtaaccac
     5641 agcaattgtc aactgcatac ctggttttga aagggcaaca aatttgttga caatgacaat
     5701 cataacgata gcaatcgtga ttgggtacaa gataaccagt actggaattg agtacttgat
     5761 aatcgcatca agacccaaat tggcaatagc aaatccaatc aaggtaaagg ctgtcgcata
     5821 aaccttgtag ctgatttgtg ggaagcgctc attaaagaac tcagctgttg acacaatcaa
     5881 accaacagtc gttgtgaagc aggttacggt aaccatagct gcaaggaaga gttgagctgt
     5941 tgagccaaag atttcttgag tggcttgtga caagatgtaa acacctggtg ttccaccctt
     6001 catcgcttca gctggtactg ggaaatgatt tccaagaaaa cctaaaccga tgtaaagagc
     6061 gctgaaggca agggcaacaa cgataccaac aacccaaata gttgaaatgt attctttctt
     6121 acttgaaaat ccaagttgtt tcaaggtttg aactgcgatt acgctaaagg ccactgaggc
     6181 aagggcgtcc aaggtattat aaccttctag gaaacctgta ccaaaggcag aagcttgata
     6241 agcagctgaa gcagcttgag gacttgttcc accatatttg atagctccca gaacgaccaa
     6301 gataacaatc aaaattgcaa agactggcgt taaaatacgt ccaatgcggt ctaagatttt
     6361 tgatggatta agcgaaatca aataggctgc cgcaaaatac agaaccgtaa atacaatcaa
     6421 gccaagtcct ttatttgcat ccgacaaaag ggggctaatc cctacttcgt aagctgttgt
     6481 agcagtacgt ggggtagcaa agaatggacc gattgacaag taaagaactg agaggtaaag
     6541 agtcgcaaac caaggcgcta tcttcgttga aatctcgtag atatatcctt taggatttag
     6601 cgttccaata ataagggtca agacggcgat accaacgcct gaaaagacaa aacctgctat
     6661 ggcaggaaga aaatgttctc cagatagagc acctagagaa ggcggaaaaa tcaagttccc
     6721 cgcaccaaaa aatattccaa acaggagcaa acctgttagg gtaccttttt tagccataaa
     6781 aatctccttc atatttataa aatactgacc tagtatacca cgattaggag tctgaaaaaa
     6841 gcatcacgag acaaatatta aatgttttca attttcttaa aaataagaat tatctaaaaa
     6901 taaaactctc ctacccagtc cacaccaagt aggagaaatg caaaatttta aagttcttca
     6961 aaggctaaca tgccaccctg gacattgata acattataac cttgttctaa caggaattgg
     7021 caagcacgcg ccgatctcat tccagatttg caaataatat aatgcaagcg atctttgtcc
     7081 aattaatcat aactatcagc caattgacta agcggtaggt tgtgggcacc ttctaaatga
     7141 agagctgcaa actcatccac ttctctcaca tccactagag aaagctggtc gttttggtaa
     7201 agctggtaaa atgcgtcaaa ggctatttct ttcattcttt ttcttctaat actcttcgaa
     7261 aatctcttca aaccacgtca gcgtcgcctt accgtgcgta tgttaccgac ttcgtcagtt
     7321 ttatctgcaa cttcaaagct gtactttgag cagcctacga ctagcttcct agtttgctct
     7381 ttgattttca ttgactataa aatggtttta attctttttt tcaaatctgg cactacttct
     7441 gcctcaaacc aaggattttt ggccatccag atttgatttc gtggtgatgg gtgaactagc
     7501 ggaaaatagg ctggcagata gtctttatag tgtttcaccc tctccgttac cttcccactg
     7561 attttctcct gtaaatagta ggcttgggca tattgcccaa tcaagagggt taactgaata
     7621 tcaggcaatt cctgtaagag ctgcggatgc catttttctg caaaacctgt acgaggcgga
     7681 agatcacccg acttgccatg tcctggaaag tagaaatcca taggcaaaac agcaaaataa
     7741 cctgaattgt aaaaggtatc ttcatccaca cctagccagt cccgcaagcg gtcaccactt
     7801 ttatctttcc agtaaaggcc tgcttcttga gttttaagtc ccggagcctg accgatgata
     7861 ttgatgcgag cagtttttgg cgctgcaaag agaggctcaa tgccacgctc tgtatagctg
     7921 gcattctgcg aatccgccat gatagcctgt ttgattcttt cgatttgaga catctacttt
     7981 ccctccaaaa agaagtccaa gcataagatc tcagacttct atcgttttat ttgatcaatt
     8041 cgtaaatagc ttcggcataa attgctgctg ctcggaagag atcgtccaag gcaataaatt
     8101 cattggcttg gtgcatggta tcaattgagt ctgggaacat agcaccatag gcaactccgc
     8161 gctctaacaa gcgaccaaag gttccaccac cgatgacttg ttcatgacct tgtaggcctg
     8221 tttgtttttc atagacattc aacaaggttt gcacaagtgg atcttccatt ggcacatagt
     8281 gaggggtatg accatgttct gatagactga cagaagcaac tggcaagttt tctaggattg
     8341 atttgatttg ttctggactt gttccttttg gatagcggat gttaagggca atggtattat
     8401 cagcacttgt ttcatcgaag cggaagactc cggcattcat agaaagagca cccatctttt
     8461 catccacatg agcaatcttg agattttcac cctcatgatc gttcaagaga attttacctg
     8521 cgatgtcaag gtagtctttg gctggaccag caaagccaaa ctggctgagg aagagggcaa
     8581 ggtaagttgc gccattgaca cctgaagcag gcatagcacc gtgggctgat ttaccaatga
     8641 tcgtcacctt gtatttgcca gcttcttctt ggagttctcc tctaagtttg tgttctgcaa
     8701 caaaggcatc tagtttagct tgcaagtcag ccaaatcacc tgaaacgact gctgttgctg
     8761 attctggtac catattttca cgtaaaccac ctgtaaagct gtgaagacgg gcagcacctg
     8821 tattttctcc tgcaaagtgg aggtattccg tgatatttcc tttttcacca ttgatgattg
     8881 gaaattcagc atctggtgag aaaccgaaat ctggtttggc aagtcctacg tgctcaaagt
     8941 agtagtccat gtctgcccag cctgattctt cgtctgttcc aacgatgaag cgaactttct
     9001 tagaagttgg aagacccaat tctttgatga ttttcaaacc atagtaacaa gctgttgtag
     9061 gacccttatc gtccgaagcc ccgcgcgcat aaaggcgacc atctttgata gttggtgtgt
     9121 aaggatctgt atcccaaccg ctaccagctg gaaccacgtc catatgggca aagattccga
     9181 gaacttcttc tccatcacca aactcaaaat gtcctgcgta gttatcgaca ttcttagttg
     9241 gataaccatc acggtctgcg atttcaagga atttttccaa ggcttttact ggaccaggtc
     9301 caaatggatg ttgggcatca gccttgctat catcacgttc tgagttgatt tccaaaaggc
     9361 taaacaagtc agccaagagg tcttctttgc gtttttctac ttctgctgta aaatcaattg
     9421 ctatcatttt ttcttcttcc tatctttttt cgatgatttc atctactggc aagcggtagc
     9481 ttggttccaa tttgtcgtct gtgtaaccca ctgtgatcaa gagttctggg cggaaacggt
     9541 cttcaatttc caaaacctca ttgacttttg atttgtcaaa accaaggata atattggaac
     9601 caattccttg gtctgtaaga gcgagaacca agttcatagc aaccaaacct gcattgagtg
     9661 ctaggtagtc gctgacttgt tgttcattgt agcgtgcaaa ttcagctggc aaatttttca
     9721 tgaagtattg aagttgttct tctgagaaat tgttagcacc accaactcgg gcaatcttac
     9781 gagcgcgttt ggcaaggtct gtatctgtaa atagggcaat ggttacaggt gctgatgata
     9841 cctgctcaaa gtttgaacca taagccaact ttgccagttc agcatttttc tcacgaacaa
     9901 ctacaaattt ccaaggctgg ctgttgtggg cgcttggcgc caaggttgca atttcgatag
     9961 ccgtacgcac atcttttgga tcaacaggct tatcagtgaa atgcttggtc gcatgacgtt
    10021 ttttatttaa ttcaagaaat ttcataatcg atttcctttt ctaattctac tgcttccatt
    10081 ctaccataat ttgaaagagc ttgcagggat ttttcatgat taagtttttc tatcacagac
    10141 ataaaagata tatattggtt tatcaggaaa gtttctgata aactagcaag tactttacat
    10201 ttgaatggag acagtatgaa aaaatttagc ctattactag ctatcctacc atttttggtt
    10261 gcctgtggga atcaagttac acccaaagag actagcgctc aaaagacaat cgtccttgct
    10321 acagctggcg acgtgccacc atttgactac gaagataaag gcaatctgac aggcttcgat
    10381 atcgaagttc taaaggcagt agatgaaaaa ctcagcgact acgagattca atttcaaaga
    10441 accgcctggg aaagtatctt cccaggactt gattctggtc actatcaggc tgcggccaat
    10501 aatttgagtt acacaaaaga gcgtgctgaa aaataccttt actcgcttcc gatttccaac
    10561 aatcccctcg tccttgtcag caacaagaaa aatcctttga cttctcttga ccagatcgct
    10621 ggtaaaacaa cacaagagga taccggaact tctaacgctc aattcatcaa taactggaat
    10681 cagaaacaca ctgataatcc cgctacaatt aatttttctg gtgaggatat tggtaaacga
    10741 atcctagacc ttgctaacgg agagtttgat ttcctagttt ttgacaaggt atccgttcaa
    10801 aagattatca aggaccgtgg tttagacctc tcagtcgttg atttaccttc tgcagatagc
    10861 cccagcaatt atatcatttt ctcaagcgac caaaaagagt ttaaagagca atttgataaa
    10921 gcgctcaaag aactctatca agacggaacc cttgaaaaac tcagcaatac ctatctaggt
    10981 ggttcttacc tcccagatca atctcagtta caataacact cttcgaaaat ctcttcaaac
    11041 cacgtcagct ttatctgcaa cctcaaagct gtgctttgag caacctacgg ctagcttcct
    11101 agtttgctct ttgattttca tattgagtat aagatatatt aaaagcgatg ggatcagcca
    11161 atcgcttttt tagtttgcat tggttatttt aggaaactct tacaaaaaat caaaaaaatt
    11221 cttcacatcc tctacatacc tatgcttttc tattaagctt gctaatagtt ctagattgtg
    11281 cctctgatag ttatcttcac gacgtagttc ttcatacatt tcgataaagg gaaacccctt
    11341 ggacttggca aattctaact cagcctcgta atcataagcg actttacgaa ataggatatt
    11401 taccaattcc ccatcttcaa cctctatcac ggcatactgg gcacggtgat tttttaacgc
    11461 ctcccaatta aaatagggca tgccaatcga ccctggattg atgatttgtt gcccttgact
    11521 accataacga agcaactact tgtgaacatg accataaact gccacgtccg tttccgcatc
    11581 tagcagttgg tcaaatttct ctgtatcatt ctcaactagc aagtcaccac catagttttt
    11641 gtcaggtaaa ttatgagaga tagaaaagcg caatccgtca atttctttct tttccagcaa
    11701 aggcaagctt cgtagccaga caatcgttgc aggatccatt cgctccatca aatactgtgt
    11761 catacgcaag agctgaactt cctgtgggtc ttctaagcca tattgcccat ctaaagcctc
    11821 aaggacacaa tcatcccaat tgcctcgaac acttgccgtg ataggaagtt cctttaacag
    11881 agcgaccaag tcatttgcac ctggaccagg gaggaaaatg tctcccatga gccaatattc
    11941 actggctcct tgagttttag catctgcaat cactgcttct agcgccgtcg cattgccatg
    12001 aacatctgat aaaattgcga ttttatggtt tattgtggtt tccttccgtt tggtaatcta
    12061 ctctttcttc cgccacttga agcaagattt ctgtttcctg cgggttcaac ttggtttgca
    12121 cagcctctgc tactgctctt ggtactccaa cttcgacaat ctcatccaca ctggcttcct
    12181 tgattttggt cagagacttg aagtgcctca tgagattttg cttgcgttta ggtcccagac
    12241 catcaatccc atccaattga gatgagaaag aatttttgga gcgcagttgg cggtggaagg
    12301 taatggcaaa gcggtggacc tcatcttgaa tgcgttggag gaggaaaaat tcctgagaat
    12361 tgcgagacaa gtccaccact tccagcggat ctccaaagag caattcatgg gtttggtgct
    12421 tatcattctt ttgcagacct gcaattggaa tatccaagcc cagttcttct tggatgactt
    12481 gcttagcaat attgacttga ccttgccccc catcaatcac aatcaaatct ggaggagtca
    12541 aagcctcacg ctgtactcga ccatagcgtc tgcgaatgac ctctcgcata ctggcatagt
    12601 cgtctggccc gactacggtc ttgattttat acttacggta atccttctta ctaggcttgc
    12661 cgttgacaaa gaccaccatg gctgaaacag gactagttcc catgatgttg gagttatcga
    12721 aggactcgat gcgaactggg gttgggattt gaagcaagcg tcctagattt tcaatagctc
    12781 cttgggtctt ctcgacagat ttctctagca ggttgaactt ctgctctagg ctaacacggg
    12841 cattttttat agctaaattg accagctgct ttttctctcc acgttgaggc ttaagaatct
    12901 tggaatccac caaggccttg acagcctctt cgtcaatatc ttgcggaatc agtacctcat
    12961 tgggaactag atgggatttt tcttgataga attgtcctac ataggtcaaa aaatcctcat
    13021 ctggatcatt gaagtagggg aagagattga catcgcgctc gatgagctta ccctgacgga
    13081 caaagaaaac ctgcacacac atccagccct tatccacata gtagccaaag acatcgcgat
    13141 tttggagatc cttagccatg acccgttgct tggttcgaag cgttccaata gcctgaatca
    13201 ggtcacggta ttccgccgca cgttcaaact ccatagtctg ggctgccgct gccattttgc
    13261 cctttagctc atcaatgatt ttgttatcct gccctttcag aaaatcagaa acctcctggg
    13321 ccatagactt gaaataaatc tcatccttct tacagatggt gtgggccatg cattgaccga
    13381 tatggtaata aaaacagacc ttagagggcg ggttggtaca cttacgaaaa gggaatatcc
    13441 gatccagcaa ccgcttgatt tcattggctg cccccacatc gggatagggt ccaaaataaa
    13501 gacctccgtc ctttttgacc tgacgagtga taatcaagcg tggatagcgc tcattggtga
    13561 ttttgatgaa aggataggac ttgtcatcct tgagcatgat attgtacttg ggcttgtttt
    13621 ccttgatcag gttgatttct aggagaagtg cctcaatatt agactccgta acaataaatt
    13681 caaaatccac aatttcagac accagagcct ctgtcttggt atcatgactt ccacgaaaat
    13741 aggaccgtac tcgattacgc agatttttag cctttcctac atagataatg gtgccatttt
    13801 tatccttatg aatgtagcaa ccagggctgg tcggcaagag ctctagtttt gatttaatca
    13861 agttgttcat agttccatta tagcaaaaaa agtagggaaa gatagtaccc tactcactag
    13921 tctcatatat ttttcggagg tttgcaacat cctcttcctg gataccataa aaggaacctg
    13981 ctggttgcat gacagacttc tcatctgtat ggtccaagcc aatcgcatga cctaactcat
    14041 gttctgccgt atggacaagg cgttcatagg agtagccata gtatggattg gacaaataat
    14101 aatgattcaa ccgcaccgtt acggacaaga attgccctgt taagagatta gtttgacttt
    14161 ccgcctctcc tgccacagga gtgcctccgt cgttcatctc cgtagccgta atatccgcct
    14221 tactagactc agtcacgagt tcaaagttaa aagcaccagt ttgattccag ttctggattg
    14281 cttctaaata agcctcttga aagcgtgaat ccatctgcgg atccaggtaa atacgagcag
    14341 aggcctgtgc ccaccttcct tcagaatgct ggtgactatc tgtctggctt aacttaggca
    14401 gttgccctgt tttctcccat tggtcaatac tttgttgacc aatttttact gaccgttctg
    14461 cttgctttag cgctccttga aaatctccgt tcagatacca gagaagtccg aaagcaagca
    14521 cacataagag cacaactatc caaaccagac gccaaaacaa acgccacaca aaagaaaaga
    14581 aagcccctat caaacgaaaa agccagcgca tgtctctcca cctttctagc aaataagaat
    14641 tattttacta aaagaagcta aaagaaatct aaatactgac tttttcatct taacgtccta
    14701 ctattttttt gagtaactgg atagctttgt attttaaagg cgatggatgg taacggaaag
    14761 tacctgcttt gcgtacaata tagccattaa aattctgttt aaaacgcaaa acaccatcac
    14821 ttccatcaaa aatcccttga atgcctagga agttgtattt aggtattcca cgttttatgc
    14881 tttccaacat aacatatttt tgaagcagtg caggggcata gaacttatta aactcagtgt
    14941 aggaaccact aaagagataa gtcgtttcct gaggcatata aacaaataaa ctcccagcta
    15001 aaacaatatc ttcttctcca tatttttcaa tcaagtctcg cgcttctgct tttcgaactt
    15061 caaacgtttc aaattgacta gaatattctc tcagttgatt ttgttttttc tcagaatgag
    15121 gatttttact caaatcaagt cgcaacttgt ccaagttttc ttctagttta ctttgttcac
    15181 cttgcaattt gctcatatag tccgaaaaat ttaagcttgc tatgagaaac tccgcttgtt
    15241 ctccaaaagt atcataaaaa tgctcataat attctaaact tttatcacta tattctctac
    15301 gttcagaggt ttcttttgtt atattcttaa aaatcgatag ttcttcacgt tttaactttt
    15361 tcaaccgaat gccaaaggtt tcagcctttt tcaccaaggg tttacccttt ttgctaaaac
    15421 ttttaagcaa actcttttca gttaattcag ttaaatcttt atagtataac caatctggtt
    15481 ctccacctgg gtaacctgtt gttaagccat caaattgata acctaaatca gtcaaacctt
    15541 gaataatact ttttttctca gcatctattg gattaccttg gctatcaaaa gtttgataag
    15601 tttcataagg ttttacaagc aactctaata caccattttg cttggcatat tcttttaact
    15661 ctgcataaaa aactggaaga gcatcttgtt gggtataaat cggccccgaa ttgagttcca
    15721 tatgcagacc acccagcatg ggcaagctat aaaccagagc tgcaacttga atttctcctt
    15781 cttgtttcaa agcaagataa acaattcgag cccctctttt ttctagcaaa tcccccatct
    15841 ggacagattg cataaaggaa cgagaagaaa cctgatcaga ataagtctga aactcttctt
    15901 tcgtgagtgt tgttagtgcc atatacttac tttctatgtt tttttcttaa tgttttacgg
    15961 aaatcaagag caagtcttaa cagaggatag agaggatgag tgggcattgt aaattcaccc
    16021 aagtattctt caatcgttgg attaaatttt tccttaaaat gataaagtcc accattgaga
    16081 gagttttcaa caccacctaa attttgccag accatacctc gctcaaaggc atagcgagcc
    16141 gtttcatacc atgttaaaat tggtgcattg taacgtttaa aatcatcatc cataccagca
    16201 tatatattga cagaggtagt accaaattcc aaactcaaag tagccgctaa aggaactctc
    16261 gcttgaccta catctatata ttcctgcaag aaggtcaatt cctctaacaa acgttctttt
    16321 tccttcttct gcgcttctac ttttgaagtt cgagtcgact cagtaaaggt ctcttccaag
    16381 gctctatttt tcgctaactg ttcttctaat tcttgcgaac gtttagaaac atccaaggtg
    16441 gccaaggtga tataggcctt gtccttaaaa ttatctaaca attttttata ataggcttca
    16501 ttcctcaaat gaatctcttt tcgcttctca gtttttttca tcaactccga aaatgaatct
    16561 aatagttcca gtccaccata ttgaatctca agccctttgt ttcgtgctgt tcgaatagcc
    16621 tgttttgttg acttggaaag tttatcttct tcaaaatttt ccttgtatat tttcgcctga
    16681 atacgaggtt gaatggtgtc tcccatttcc tccgtttttc ctgaccacct tactcccatt
    16741 tgttgcaaac tatcaataat agccagattt tcaggaaatt ctgtcttttc ctgattgatt
    16801 aaactttgag ataggcaaat acttgggtca aaagtcacaa aaaccactct cttactgcga
    16861 gcataggact taatagactg aatggcaaaa ttcaagagtt ctttatcccc ataatccaat
    16921 ataggtcctc ttgggatgta aaacatttta tagcctagcg gaagagttct aatcaaaata
    16981 ctagctgtcg ccagtaattt ttcttccctg taaacaccaa acttctcatg ttgccaatta
    17041 gacttaactt cctcccaagc actactttgt aatacattgg ctaattcatg ttctttgaca
    17101 aactgatcat attctaatgt gggaatgcca atttgataac ggtacatttc ctactctctt
    17161 tcctccagta ttttattact attctactac tttcgtttga aaagtctagt gaaaacatat
    17221 tttattttag ttctactccc cctatgctga agttcaaact aagtcagtct ctcttctaat
    17281 ttgaaatcaa ttggtagggt tgttaaaaaa acttccaatt gtttttccca gtagtaccac
    17341 tcgtgagttc cagcgctatg gctataggtc acatcaaaac ctagtttttt gagatttttc
    17401 actgcgagat tattggcttc gtacaagaaa tcctgttcgc cacaccacgc ccaaagtttg
    17461 gtctttttat ccgatttttt agccagactt tcaagagaat agggactagt tgtccagtct
    17521 ctaatctctc caaaaacacc tctccagtag gctggacttc ccagattttg actttcagga
    17581 gaaaagtttt gaaagctgag ggcacctgaa aaactagctg catgagaaaa acgatttgtc
    17641 gtaagagcca gtttgaagca gccgtagcct cccatagaaa gaccagcgat aaaggtcttt
    17701 tcacgcttgc tcgtcatatt agggaagaag cgtttcagaa cctgtggcaa ttcctctgct
    17761 agagccgtgt agtagtcaaa accatactgg gtatcggtgt accaaccatt gctggtattg
    17821 ggcataacaa cgatgagatt agttcctcga agcaagcgtt ctacattggt ccgcttaagc
    17881 caactattat gatttccaga catcccgtgc aaaaggtaca agacgggaat atcttcacac
    17941 tctggttctt ccactcgatt ggcatcaggg tagaggacat tcacccccca ctccatatcc
    18001 aatacttgtg agtaatactc gattttcatt actgccatga ttttctcctt ctatttttct
    18061 ataagaaaaa gccgaaactc gactttctct ctgtttattt caacgattat tttgcttcca
    18121 tgactactgg taaaatggct gggcgacgct tggtttgatc aaagaggtac ttggtcagat
    18181 tgtcacgaac cttacctttg agatctgccc agtcaaagtc atctccttga agatactctt
    18241 ctaccgtttg gttaatcaat tctgaacttt cacggagaat atcgcgactc ttcttgagat
    18301 aaacaaatcc acgcgtgtga acacgagccc tagccacaat tttcttctca cgacggttga
    18361 ctgtaatagc cacgatgaaa attccatcct ctgacaagac cttacggtca cgaagaacaa
    18421 catttccaac atcaccaatg gcattcccat caatcaagat atctcctgct gaaaccgatc
    18481 cagctggaac aaagtctcca ttctcgtaag ccatggtcgt ccccttttta ggaatgaaga
    18541 tgcgttctgg caacatccca actgccatgg cagccttagc gtgagcatcc aactcacgat
    18601 actccccttg gacagggaag aggtacttag gttgcaaaag attgatcatc agctgcaaat
    18661 cacgcacatt tccgtgccct gatacatgta aactttgggt aatcaatttg acaaccccac
    18721 ctgcctgata aatcatattt tccacacgcg caacaaaggc ttctttagca atagacggag
    18781 ccgtagcaat atagaccagg tccccatcct tgatttctac ataacgatgg cgaccaatcg
    18841 acatcttacg aagtccattg ataggttcac ccatacgacc tgtctcaaga ataatcaact
    18901 catggtcttc aaagcgagac atatctttag gcttaatcaa gagaatttcg ttggctaaag
    18961 acaacttctt aagacgaatc gctgtgcgga cgatattttc aatatcaaat cctgtcaaga
    19021 cgatacgtcg acctgtttta tccgcagcgt caaaaatctg ctgaatacga gaaagattac
    19081 tggaaacagc tgcaacgatg atacgacctt cccagtcagc aatagtttgg gtaatttcat
    19141 ccctaacttc actttcacta gccacctgaa tattgctgtc tgcattggcc gaatcactga
    19201 ggagagccag gacgccgtca cgaccaatct ctgccaaacg agcaaaatca gttgcataag
    19261 attcactagc cgtttggtca aatttgaagt cacctgtata aacgatgctt ccttccgatg
    19321 tcttcaagac aattcccaga ctctctggaa cggagtaagt cgtagggaag aaggaaacca
    19381 ctgtcccacc aaaatcaatc tccgtattct catcaatgac atggaaatca ttaaatttct
    19441 taacggcatc atttcctttg acaaagagct ttgccaactc aatggtcaac tcagacccaa
    19501 atacaggaac tttagcctct gccaagagat aaggtagagc accaatggca tccgcatgcc
    19561 cgtgggtcaa gaaaacccca gcaatacggt cgctattttc aaaaaggtaa tccatgtttg
    19621 gaatcaccac atcgacccct aattgttcat tttcaggata ttttaaccct acattcaaaa
    19681 caaaaatgga ctctccaatt tcagcaatgt acatattttt tccattctca cgcacaccac
    19741 caagtgttgt taaactgata ttactcattt ttcctccaaa atcttagttt atcttgatta
    19801 tagggcaatt ctaccctctt attctaaaag ttctgatact ttcagccgaa tcttttccta
    19861 ccattatacc atttttttga tgattttcaa aatgaaggtc tgttttcaaa aaagagttga
    19921 tttcccaact ctttcctaac ttctactctt tttccatcaa aaaatcataa atttcttgca
    19981 ccgtacccag cgccatcatt tcttggtctt taacggtttg tttgagattt tggtaaattt
    20041 gactttctcc tatctcccgc tttggcgcct ctttattgac tgtcatcaca ccatctttta
    20101 tcgtcacaaa gcctagttct tcaaatactt gaatcatctt gaccagcaag atttgttgaa
    20161 tattaagata tgcagccaaa tctttcagct tgtagcgaat atcaaactct gggaactggt
    20221 aaatagtctt gtacaatttg gcaaactgat ctctagtccc ataacctgtc agataataag
    20281 ccttgtcaat atcatttttg aaatagacag cagagaaatg ctgttcctga aaaatggtct
    20341 tcagctgagt aatatcctct ggaatgtttt ttacgacaac agcttcacta gccgcaagat
    20401 ttggcagttc tccaggaaaa tccaagactg gaacaccttc tggcaagact gcattttttc
    20461 cacgaatgtt aaaaagctga acaccttcca ctcgcgcatc caccatcatc aactggaggg
    20521 cagtttggcc attccattgg ttgacagaca atttaaccgc taactctaga ttcttggttt
    20581 gagaaaactc tgtcgcccat ctgccttgac caaaggctac cacttcaaaa ctcgcctcac
    20641 ccttggaaat tttcagcttt agatgggcat tacctgcccc catagtacga gcactttcga
    20701 cctgaaaatt cttgatataa aaaataggtt tctgattatc cattccaaaa ggagctaaac
    20761 gttcaaaact tttgaccgtt tccaagctaa gtgcctccaa atccaactct tcatctaggt
    20821 ttaacttatt cttgccacca gcatctgcac ctttttcacg aacataatct tccaaaacct
    20881 gagataaatc tgagagttgc tcaacttcca gcgtcatacc cgctgcacct gcatgacctc
    20941 caaaggcgat gaagaggtct cgatagggat ccagagcttc aaaaatatcg accgcttcca
    21001 cactacgagc actgcccttg gcacgaccgt cttctatatt aagaacaatg actgtctgtc
    21061 ccaattcttc caataaacga ccagccacga ttcctagaac cccaggattc cagccttcct
    21121 tggccaagac ctgaaccttc ttctcaggat ccaccatggt cttggcttct tcatagatag
    21181 actgaacgat ttccttgcgc tcttcgtttt tctggtgaat cataagggca atctcatgcg
    21241 cttcctcatc atcaaatcca gtcaacaaat caatggcagg attgggatca tccaagcggc
    21301 ccaaggcatt caaacgagga gcaatctgga aaccaaccgt ttcttctgtt acttcgttgg
    21361 cagcaatccc agccatgtcc agcatttctt gcagaccaat gcgctgggta tgacccaaca
    21421 tttccagacc atattgaact aagatacgat tttcatccgt cagactcacc atatctgcaa
    21481 tagttccaat agcgaccaaa tcaagcaatt ccacttgcac ttcttctaac agggcacaag
    21541 ccaacttgaa agcaactcca caaccagcca aatatttaaa aggataatcc gcatctggat
    21601 gttcaggatg gacaatagca taagcatctg gcagggtttc aggcatggaa tgatggtctg
    21661 tcacaatgac atctactccc atagactgag ccaatgcaat agcctcatga ccagcaaccc
    21721 cattgtccac cgtcacaatc aaggaaattc cttcttgctc gataaagtat ttataaacac
    21781 tagcattagg gccatagcca tcggtaaaac gatttggcag gtaaactcgg cactcagcac
    21841 caagttgttc caaactttcc ttcacaatag aagccgaagt catgccatcc gcatcatagt
    21901 ctccataaac gagaatattt tccccttctt caatagcctg acgaatccgc tccactgcct
    21961 tgtccatatc atggagcaga taagcatcat gtaagtcctc caaggaaggt tctaaaaact
    22021 tcttcagact ttcttggtcc tgaatccctc tctcaaacaa taaccgagcc acctcaggac
    22081 ccagtccagc cttcttggct atctttgtaa aatccgcatc ttctacctgc agggcaaact
    22141 gccattcata agtaggtgtt atcaaaaaga catccactct ttctaagaat tctattgctt
    22201 cattataaca tgatttagac gttttctcta tccagattgc ttttttataa cattttatag
    22261 tatattgaaa ctagaatagt acacctctac ttctaaaata ttgttagaaa tcgatttgac
    22321 tgtcccgatc gatttgtcct gttcttattt cactatagtg aattttttca gatatggttt
    22381 gacaagaaca ttcttttatt gtagaatcgt gttacaaaat ctaacataaa tgagatctct
    22441 catggcttta gtagaattta aaaacgtcga aaaatattac ggagactacc acgcactccg
    22501 caacatcaat ctccgttttg aaaaaggaca agttgttgtc ctgcttggac cttctggctc
    22561 tgggaagtcc actcttatcc gtacgatcaa tggtttagag gctgttgaca aaggaagtct
    22621 cctagtcaat gggcaccaag ttgctggtgc cagccagaaa gatttggtac ctcttcgcaa
    22681 ggaagtcggc atggtttttc aacattttaa cctttatcca cacaaaacgg tgttagaaaa
    22741 cgtgacactt gcacccatta aagttctagg aattgataaa aaagaagctg aaaaaaccgc
    22801 ccaaaaatat ctggaatttg taaatatgtg ggacaagaaa gattcctatc ccgccatact
    22861 atctggtgga caaaaacagc ggatcgccat cgctcgtggt cttgctatgc atccggaact
    22921 cctcctcttt gatgaaccaa catctgctct tgatcctgag actatcggag atgttctagc
    22981 agttatgcag aaactggcgc atgatgggat gaacatgatc atcgttaccc acgaaatggg
    23041 ctttgctcga gaggttgcgg accgcattat ctttatggcc gacggagaag ttttagtaga
    23101 tacgacagat gtcgataact tttttgacaa tccaagcaaa cctcgtgccc aacaattcct
    23161 cagcaaaatt atcaaccacg aaagtgacaa agtcaaataa ggaggcgcct atgaaaaaaa
    23221 ttctttttat cagcattatt gattagcctt ttcggccttg ctgctgccaa accagtccag
    23281 gctgatacta gtatcgcaga cattcaaaaa agaggcgaac tggttgtcgg tgtcaaacaa
    23341 gacgttccca attttggtta caaagatccc aagaccggta cttattctgg tatcgaaacc
    23401 gacttggcca agatggtagc tgatgaactc aaggtcaaga ttcgctatgt gccggttaca
    23461 gcacaaaccc gcggccccct tctagacaat gaacaggtcg atatggatat cgcgaccttt
    23521 accatcacgg acgaacgcaa aaaactctac aactttacca gtccctacta cacagacgct
    23581 tctggatttt tggtcaataa atctgccaaa atcaaaaaga ttgaggacct aaacggcaaa
    23641 accatcggag tcgcccaagg ttctatcacc caacgcctga ttactgaact gggtaaaaag
    23701 aaaggtctga agtttaaatt cgtcgaactt ggttcctacc cagaattgat tacttccctg
    23761 cacgctcatc gtatcgatac cttttccgtt gaccgctcta ttctatctgg ctacactagt
    23821 aaacggacag cactactaga tgatagtttc aagccatctg actacggtat tgttaccaag
    23881 aaatcaaata cagagctcaa cgactatctt gataacttgg ttactaaatg gagcaaggat
    23941 ggtagtttgc agaaacttta tgaccgttac aagctcaaac catctagcca tactgcagat
    24001 taaggaggac accccatgac agatttatca tcttggacag cctattttca ggattttgga
    24061 caatttttca atggtttcct cttcaccctt gccctagcgg ttggttcctt tatcctcgct
    24121 atggttttgg gcatcttctt tggtgccatg tcaaccagta aacgtccaat tttacgcatt
    24181 ttagctcgca tctttgttga attttaccaa aatactcccc tcttggtgca gtttgttatt
    24241 gttttttatg gcctacctct tatcagtgac catatcatca tgattccaat ttattggaca
    24301 gccgttctct gcgtgggact ctatcacggc gcttatatcg ctgaggttat tcgttcaggg
    24361 attcagtcta ttcctagtgg tcagatggag gccgccttgt cgcaaggttt tacctatatc
    24421 agtgccatgc gcttgattat cttgcctcag gccttccgca tcattctccc tccattgacc
    24481 aaccaaattg ttaacctcat caagaacacc tctactgtag ctatcatctc tggagtagac
    24541 ttgatgtttg tgactaagtc ttggtcggct ctcaacggaa actatatacc agccttttta
    24601 ggtgctgctc ttctctactt tgctctatgc ttccctgttg cccagtttgg tcgcaagatg
    24661 gagcaagcca acaaaaaagc ctattcactt taggaggtta ctatggaatc tattttagaa
    24721 gttttaaccc cagataacct agtctttatc tttaaaggat ttggcttgac cctctatatt
    24781 tctctgattg ccatcatcct ctctactatc atcggtacgg tgctagctgt cacgagaaat
    24841 ggcaaaaatc ctgtcttacg cattatttcc agtatttata tcgagtttgt gcgcaacgtt
    24901 cccaaccttc tctggatttt tactatcttt ttggtgttca aaatgaaatc cacaccagca
    24961 ggtattacag cctttactct ctttacatca gcagccttgg ctgagattat tcgaggcggt
    25021 ctcaatgccg tagacaaggg acagtacgaa gcaggaatgt cacaaggctt cacctcagcc
    25081 caaatcctct actacatcat tctcccacaa gccatccgca aaatgctacc agccatcatt
    25141 tctcagtttg ttaccgtgat taaggatacc agtctcctct actctgttat cgccctacaa
    25201 gaactctttg gagccagcca aattctcatg ggccgttatt tcgaaccaga gcaggtcttc
    25261 agtctttaca tcctgattgc cctcatctac ttcagcttta acctagcaat ttctagcctg
    25321 tctcatatgc tagccaaacg ttggcaacaa gctgcagaat aacactcttc gaaaatctct
    25381 tcaaaccacg tcagctctat ctgcaacctc aaaacagtgt tttgagcaac ttgcggctag
    25441 cttcctagtt tgctctttga ttttcattga gtataaacag ccactctcca gttcaattgg
    25501 agagtttttt gatgagagta gatatttata gacctcaatt tgacaccata cattttctat
    25561 gataaaatgt aacaaattta tttacaacaa atcgaaatct aaatgaaatg gaattttcta
    25621 tgaaatcagt caaaggacta ctctttatca tagctagttt tatcttgact cttttgactt
    25681 ggatgaacac ttctccccaa ttcatgattc caggactagc tttaacaagc ctatctctga
    25741 cttttatcct agccactcgt ctcccactac tagaaagctg gtttcacagt ttggagaagg
    25801 tctacaccgt ccacaaattc acagcctttc tctcaatcat cctactaatc tttcataact
    25861 ttagtatggg cggtttgtgg ggctctcgct tagctgctca gtttggcaat cttgccatct
    25921 atatctttgc cagcatcatc cttgtcgcct atttaggcaa atacatccaa tacgaagctt
    25981 ggcgatggat tcaccgcctg gtttacctag cctatatttt aggactcttt cacatctaca
    26041 tgataatggg caatcgtctc cttacattta atcttctaag ttttcttgtt ggtagctatg
    26101 cccttttagg cttactagct ggtttttata tcatttttct atatcaaaag atttccttcc
    26161 cctatctagg gaaaattacc catctcaaac gcttaaatca cgatactaga gaaattcaaa
    26221 tccatcttag cagacctttc aactatcaat caggacaatt tgcctttcta aagattttcc
    26281 aagaaggctt tgaaagtgct ccgcatccct tttctatctc aggaggtcat ggtcaaactc
    26341 tttactttac tgttaaaact tcaggcgacc ataccaagaa tatctatgat aatcttcaag
    26401 ccggcagcaa agtaacccta gacagagctt acggacacat gatcatagaa gaaggacgag
    26461 aaaatcaggt ttggattgct ggaggtattg ggatcacccc cttcatctct tacatccgtg
    26521 aacatcctat tttagataaa caggttcact tctactatag cttccgtgga gatgaaaatg
    26581 cagtctacct agatttactc cgtaactatg ctcagaaaaa tcctaatttt gaactccatc
    26641 taatcgacag tacgaaagac ggctatctta attttgaaca aaaagaagtg cccgaacatg
    26701 caaccgtcta tatgtgtggt cctatttcta tgatgaaggc acttgccaaa cagattaaga
    26761 aacaaaatcc aaaaacagag cttatttacg aaggattcaa gttcaaataa gtaatcatac
    26821 tctcctggga tttcccagga gagtttttta tagccaaatg aaataagaca ggaacgatgg
    26881 aattttggca ttcaaatcaa ttgataagac tattttatag tggagtgaaa taggatacga
    26941 acaaattgat taggaaaatc aaatgaattt atagaaatct tttagcagtt atgttatcct
    27001 attctagttt caaaacgcta tagaagcagc attgtgctag tctagattca gtttactata
    27061 ctaaaacgag tagcttgaaa tcaaaaaacc caccctcaca ggcaggtttt atctgtatta
    27121 ttcagctaga ttatgcttta ccttctgaac cgaatacgtc gatacgttct tcaaccgatg
    27181 cttggatagc ttttacaccg tcagccaaga atttacgtgg gtcgaagagt tttttcttgt
    27241 cgtattctgc ttcgtttgct tcgtagtcac gagcaaattt acgagttgcg ttagcgaatg
    27301 cgatttggca ttctgtgtta acgttaactt tggcaacacc aagtttgata gctgcttgga
    27361 tttgctcatc aggaatacct gatccaccgt gcaatacgat tgggaatcct ggaagagctt
    27421 ctgtcaattt ttgcaagtgg tcaaggtcaa gaccttccca gtttactggg taaggaccgt
    27481 ggatgttacc gataccagct gccaagaagt cgataccagt ttcaaccatt gctttagcgt
    27541 cttcgattgg agccaattca cctttaccga tgattccatc ttcttcacca ccgatagtac
    27601 caacttcagc ttctactgag atacctttag cgtgtgcttt ttcaacaact tctttagcca
    27661 atttaaggtt ttcttcaact ggaaggtgtg aaccgtcaaa catgattgaa gtataaccaa
    27721 cttcgataca ctcaagtgca tcttcgtagt gaccgtggtc aaggtggata gctactggta
    27781 cagtgatacc cattgattca acaaggttag cgatcaagtt gcgagcaact ttgtaaccac
    27841 ccatgtattt agcagcaccc attgaagttt ggatcaaaac tggagctttt ttagcttctg
    27901 ctgcgcgcaa gatagcttga gtccactcaa ggttgtttgt gttaaatcca ccaactgcat
    27961 aaccgttgtc acgggctgct tggacaaatt tttctgctga aacgattgcc attttatcag
    28021 gcctcctgta tatttttatg ggtcatccca tttacattgt tcattttatc actttttgcc
    28081 aaaaaaatct agtttttccc gcagtttcga ttgattttct tctaactcca tctatgtaaa
    28141 ccctttctct ccctagtctt ggacgacttt tggaaaatct ataaagaagg ttaaactatt
    28201 ctcctccatc tcgaaacgat aagctaattt ttcatgttct aatagactct taaccacaaa
    28261 gagccccata ccagacccct tgaccttgcg actggcattg tcagaaaaag actgggctag
    28321 tttttcttgt tcctctgagc tacagctatt ttcgataaaa agttctcctt ctctttctcc
    28381 aattcgaact aagccacctg gaacagagtg cttaatggca ttgctgatga gattagaaag
    28441 aatcaacttc ataactgatg ggtttagata agcctgctga tgggtcaaac tattgtctat
    28501 ctggagctct ctttccttgg ctagcaaggc ataatctttg accagatttt gcgtcatctg
    28561 gaggaggtca attgtttccc tatcatctcg caattcctgc acagaagaga gggaaagtat
    28621 ctgcagaaca tggtgattga gttcatccac aatccccaag gcaactccca gatactggtc
    28681 tctatcctta taacgaccga tattctctct catattttcg attaggattt tcaaactagc
    28741 cagcggtgtt ttcaattcat gagaagctcc tcgtaggaat tcgaccttca tcttctccag
    28801 ctggagaatg gcttcattct tttcatgcaa gtccgcaata acagtcaaga gatgctggta
    28861 gaggctattg atttgttcct tgagattacc tatctcatcc ttagaatcca cgcgcaatcg
    28921 cacttgggaa tccaggtcca tcatccgacg ggtcacccgc ttgatttcca aaatcggtgc
    28981 aacaatagtc cgagcgtaga tgtaggccac caaaagggaa atcagaaagg aggccagcaa
    29041 ggtataggga agaaactgga gactgatttg ctccgcttcc ttttgtaaat ccatggaagc
    29101 tagaaactgg agaatcatag taccaccgtc ttgcgttttc acctcgcgct cctcaataaa
    29161 gagagaggtt gtctggcggt ctgtgtccag aggaagactg tccttgactt ctaacttgtc
    29221 ctcggtcatc tcacctttga cggtcccctt gatatcacta gtctgggaat acaagtctaa
    29281 cacttgctcg atactctgcc tatctttccc ttctagggac tgggcaatgg ctgttgcctt
    29341 ttgaccaatg gtttcctgac gatgactcag ataagtcgaa ggaaaaagaa aataaatagc
    29401 taaatgaagg cagataacca gaacactaaa tatcgagaag gtatagataa atatctttgc
    29461 aaataaacct gttcgtttca ttttcgctcc aatttataac caacattgcg cacagtgagg
    29521 atacaatcca agtctagctt tttccgcaat tccttgatat aaacatcaat aacacggtca
    29581 aagggaacct catctgtcgc tttccagacg gcatcgataa tctgagaccg agtcaaggcc
    29641 cggccttcat ttttcactag atagtccaga atttccaact ctttggcatt gataggcact
    29701 tcttgacctg cgaggcttgc actgtagctt tcaaagtcca ccttggtatc cttgtaagaa
    29761 aagattcgtc ctgtatcgta gtagcgcttg aaaatcgcgt ccactctcac ttttaagagg
    29821 gagagggaga aaggtttttc cagatagcca tctgccaaag aggcaaaggc actcatcttg
    29881 tattcctcat cctgaaaagc tgtcaacatc aagacaggaa cctgactggt tttacgaatc
    29941 tcagctagaa cttctaagcc gttaagcttg ggcatctgga tatccagtaa aaccagggcc
    30001 acctcatagc tagaaaattg ctccagagct tcctgaccgt ccgctgcctc aatagtttca
    30061 tagccacaat ccgtcaaata atcactgacc ccctcacgga tcatctcttc atcttctaca
    30121 attaaaattt tcatacttta actgctctct attttttatt tttcttagaa taaataccta
    30181 ctctattttc tattatagtc tcttgctggc gttttgtctg taagtaactg atcactagat
    30241 aaaacgttgt gaaattcctt tctcataaat tccatagctt tagtatatta tatttaagca
    30301 ataaagtaca aagaaaacaa ctgaaagcaa tgattttcac cactgctttc ggatttattt
    30361 tgaattgtta aatagccatt cctatccact attcttgaat agaaacacaa gatgcaatct
    30421 ttattccaga ctcatttttt caaatttatt caccatccag caagagctct tttggttgtt
    30481 ttctaaggag attgcttgaa gcaagcgcca taacgagaac cactagaacc aaggcaagga
    30541 caaaaatgat gataaagtct gatgtctgaa tagaaatgtc taggctcgac aaggtcttgc
    30601 taaagccatc tacttctgca ccaccaccaa ggttagaggc ttgagccgcc ttactagcct
    30661 gtttggcaac acctgaagtc acattggcaa ggacagtgtt tccaattgca cgggcagtgt
    30721 aattagctag gaagtaagca gaaactagag cagggatagc aatcaagata gattcggtga
    30781 tgaattgacc caagatactt gcctgcttga ggccgataga gaggagaatt cccacttcct
    30841 tgcgacgggc gttgatccaa aggctgagca agagggcaag gaggagaact gagaagctca
    30901 agctacccca gaagaggagg ttggccatct tgtacatacc agagatagat tgctcaagag
    30961 ctgggtagtt agaggagctc ttgacgagtg tgtagctctt ccagttgata ccactgatgc
    31021 cattcaactc tttcataaca tcatccaagt tcttgtctgc tgttacaaag aaggttgcgt
    31081 ccccataaat ggctgtgtct tctgtgtatc cataaagttt tgcagcagtg tgaatgtctg
    31141 taatagctgt gttttcgtaa agttcttgtg agtaggttac tgctgactta ttatgaccat
    31201 caaagagtcc cttgattgtc acttcaactg tttccttggc tcctttttca ttatctgcat
    31261 cgtagatatt agagtccagt ttaaccttat cccctacttt ccagccgtgt ttggctgcca
    31321 agtccttgtg caagaggatt ttatccttgt cgtcgttggt taagtgctct ccttcgacta
    31381 gtttataaga accagagaca aacttgtctt ctttagagga gtcattgaca cctgtaatca
    31441 tcaagctact tccaaaacgt ttggcacgat cagcagtgag attcttcttg gtttctggcg
    31501 tttcaatcag gtcatatcca gtcaaatctc cgatagcgtt gatacgtttg acataagact
    31561 caatggcctt gttttcggtg atttttttga tgtcttcacc cttgatattc ccagcaccac
    31621 gaggcgttcc ttggttgacg cgacgattga tttgcatgga gaagctattg gtgatatttt
    31681 taaaggtctc ctgagaagcc ttggcagtag ctcccttgat tgacaagccg accaaactca
    31741 agctcgccat gaggagaata atcaggaaga tgacaatcga tttgaaaaac ttccttgtaa
    31801 cataggcaaa tgcgttgtgt aacatagatt ccctttctag attttgtttt aatcattcta
    31861 ttaaagtaag ctcaaattat ttactagtat tgcgcgtttc agtcagtttc ttatccttta
    31921 attcaagtgt aatatctgac gcttgtgcca cttctttact gtgagttacg acaatcacac
    31981 atttacctgt tttctgggca agtgatttga gtagttcgac aatatctcca gcagttttag
    32041 gatccagatt tcctgttggc tcatcagcta gaataactgg agcttctgag accaaactgc
    32101 gagcaatggc aacacgttgc tgttgaccac ctgataactg gagaacattc cgcttgatct
    32161 ggctttcatc caaaccaagc tcaagaagtg tatccttgct tgcctttttg ttgaccaagc
    32221 ggatattttc cagcggagaa agataatcta tcaagttata attttgaaag accagggaaa
    32281 tatggtgcat gcgatggtaa gaatagccct tcttacgaat atcctctcct tgaaaaagga
    32341 tagaaccttc aacaggacta tctagaccag caagtaggga caagagtgtg gattttcctg
    32401 ctcctgactc cccaataata ctgtaaaatt ttccgggttc aaaattataa ttgatctgat
    32461 ataggactgc ttcagcagta ttcttataac ggtaggtaac atcttgtaat tgtaataaag
    32521 tcatgatttc tccttcttaa ctaatagatg ataaaatttc tttcggtgat tttctaaata
    32581 agaataggaa acaaagggct acagataagc aactaagcag aactagaaaa acataggatt
    32641 ctgcaaaaga taagatgcta gttgataaac tgcttgcttt ggctagtgta tcttgtaagc
    32701 ttgcctgatc tccacttgct agtagagttt ggagtaggta agttgtgatt gcgtttcctg
    32761 caacaaatgc tggaagcaaa gctccaagag ataccaaaac tacctctaaa cagaattgta
    32821 ggaagatcga gctcttgcct tttccaagtg caagtaaaat ccccacttca tagacccgtt
    32881 ctctcaacca gagagacaaa accagaatta aggctccagc tcctgctatc aacatcccat
    32941 aaaggaagat ggtcaggaag gtttggaaag ttgcaactga gtctttgatt tgttcaaaag
    33001 ccttgttttc cttttcgact tggtagcctt gattttccaa ggccaagttt tctacctgct
    33061 tcatgagtcc gtccatttcc ttaggatttt ctacatagaa gcgtgctgca ctgacttgag
    33121 cttcactatt gcccaaaagg gtttggctac tttcatagtc tgtaaagact tgattttcac
    33181 tgaagtcaga agacaagcct gtgaatttct cttgtttttt accagaaaag atgccgataa
    33241 tctcaaactc tactgtttgt ccttttccag attcagactg accagcatcc aagccaatct
    33301 tgtcatgaag cgaaagaccg ttcttcttag ccaattcttc gtggataagg attttcttgg
    33361 aatccccttt ttgaaggtgt cgcccttctt ttagattgaa agccgaactg gtaaaggtta
    33421 catccttgga tgaatcctca agagccgtta agctaaccaa gttattgtct gcagctgata
    33481 aatcatcacg ctccacgctc tgctcgccag tcactgcttc cttgtctttt agttttgcga
    33541 ccgtctcaag ttcaggagag acattttcca gccccttaat cttgcttaca gatgctaggt
    33601 ctgacaactt gaatgtctga ccattctcta tcttcttaat agaaaaagat gtattgagtg
    33661 atttataaag attgctttct actgttttgt tggacttcat cagagtcaaa caggctgaaa
    33721 ttccggccaa taagaccaat aaaatcagaa ataaaataaa acttctcagt cgctttctgc
    33781 tgacataagc ccaagatctt tggattggat tcatttgtca cctccatatt tgtaagacta
    33841 ttataaaacc caaatatgaa atatttatga aatacgaaaa aaaaatatcg agtaggggat
    33901 aatctctagc ccctctcaca ccaccatacg tgccgttcgg catacggc
//
LOCUS       DAVHEX010000017        33700 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVHEX010000017 DAVHEX010000000
VERSION     DAVHEX010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33700
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.017"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(175..1398)
                     /locus_tag="VVL45_000793"
     CDS             complement(175..1398)
                     /locus_tag="VVL45_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEU4186994.1"
                     /translation="MITKQSAFLQNRATILEFLYRNPATSRTDIVNETGLTPATTTNI
                     IKELSEQSLIYETGDEFSEFSGSGRRRKTISITDNIPYVVGGIEINVLGIFLSLCDLQ
                     GKTLFETEILNEDYPISEINSTITNMIKTAIEYVPLETKLLGFGLSIPGHYNKDSGSI
                     ITNNPIWESFNLLNVIKRFNFPFIVKNNIDCMAIGQYLFNPHNTPDNFIFLHAGLGIY
                     TSFFTKEKIGASKNPYIGEIGHTIVELNGQYCECGKKGCLQTYISDAWLIKHAQLLFK
                     NSQLTVLKSLVKTEKDIHLDTLLTAYNLGDSALRQQIDKGVNLLATSIANLLLINPAD
                     KIYINSQLLNYQPFTHEVRDKIQDQLHFVPFTRNIEIEILPYNKHRGSIGACALAIVA
                     FFIEHSNVLQDIISP"
     gene            1566..2888
                     /locus_tag="VVL45_000794"
     CDS             1566..2888
                     /locus_tag="VVL45_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU4186995.1"
                     /translation="MNNILAFLETKVAPFGEKVGNQRHLKAIREGFMMAMPLILVGSL
                     FLILISWPQEAFTNWLNSVGLLSILTTMNQSTVAIISLVACFGIAYRLSEGYGTDGPS
                     AGIIALSSFVLMAPRFSSMVYDKNGEQVKQLFGGAIPFSSLNASSLFMAITIGLVTAE
                     IYRMFIQRGITIKMPSGVPDVVSKSFSALLSGFTTFVLWALVLKGLEAAGVAGGLNGL
                     LGAIVGTPLKLIAGTLPGMILCVIVNSFFWFCGVNGGQVLNAFVDPVWLQFTTENQEA
                     VAAGQTLQHIITLPFKDLFVFIGGGGATIGLAICLFLFSKSRANKTLGKLAIIPSIFN
                     INTAILFTFPTVLNPIMLIPFIATPTINALITYVSMAVGLVPYTTGVILPWTMPPIIG
                     GFLATGASWRGALLQVVLILVSVAIYYPFFKIADKRNLEKEKATVGGK"
     gene            2890..4776
                     /locus_tag="VVL45_000795"
     CDS             2890..4776
                     /locus_tag="VVL45_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HEU4186996.1"
                     /translation="MVIRVFDQQKNTYSSFALEELSYYMNRVFKTNIELVEEKEADIF
                     VGLVNKEDRKDHVLISLDKGKGRIESNTIVGLLIGIYRMFHEFGVVYTRPGRRHDFVP
                     ELRFEDFLDKQLSIDETASYYHRGVCIEGADSFENILDFIDWLPKIGMNSFFIQFENP
                     YSFLKRWYEHEFNPYLNKEQFSNELVQELSDRLDKELQKRGLIHHRVGHGWTGEVLGY
                     SSKFGWESGLSISEEKKPYVAEINGKRELFNTAPILTSLDFSNPDVADKMVEIIKDYA
                     KKRPDVNYLHVWLSDARNNICECENCRQELVSDQYIRILNQLDRALTSEGLDTKICFL
                     LYHELLWAPQKEKLDNPERFTMMFAPITRTFEMSYADVDFDNSIPTPKPYMRNKIILP
                     NSLEENLSYLFEWQKAFKGDSFVYDYPLGRAHYGDLGYMKISQTIYRDVSYLSNLHLN
                     GYISCQELRAGFPHNFPNYVMGEMLWKKTRSYEELIEEYFSALYGENWQSVVEYLEKL
                     SIYSSCDYFNAIGSRQSDVLANHYYIAYNLADNFLPIIEENISKLLNSQKDEWKQLSY
                     HREYVVKMAKALYLQATGKTRQAQDEWRNVLNYIRGHELLFQSNLDVYRVIEVAKNYA
                     GFHL"
     gene            4944..5288
                     /locus_tag="VVL45_000796"
     CDS             4944..5288
                     /locus_tag="VVL45_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU4186997.1"
                     /translation="MKSKEEINMLGFTIVAYAGDARSDLMDALAFARDGYFEQARELV
                     ESANDSIVSAHREQTNLLAEEAYGDNFEVSFIMIHGQDTLMTTMLLYDQVKFFIDEYE
                     RIRKIEEHIGLQ"
     gene            5298..6710
                     /gene="lacG"
                     /locus_tag="VVL45_000797"
     CDS             5298..6710
                     /gene="lacG"
                     /locus_tag="VVL45_000797"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEU4186998.1"
                     /translation="MENLQVKALPKEFLLGTATAAYQVEGATRVDGKGINMWDVYLQE
                     NSPFLPDPASDFYYRYEEDIALAAEHGLQALRLSISWVRIFPDIDGDANVLAVHYYHR
                     VFQSCLKHNVIPFVSLHHFDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWF
                     TINELMSLAAGQYIGGQFPPNHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGKVGC
                     IHALKPGYPIDGQKENILAAKRYDVYNNKFLLDGTFLGYYSEDTLFHLNQILEANNSS
                     FIIEDGDLEIMKRAAPLNTMFGMNYYRSEFIREYKGENRQEFNSTGIKGQSSFKLNAL
                     GEFVKKPGIPTTDWDWNIYPQGLFDMLLRIKEEYPQHPVIYLTENGTALKEVKPEGEN
                     DIIDDSKRIRYIEQHLHKVLEARDRGVNIQGYFIWSLQDQFSWANGYNKRYGLFFVDY
                     ETQKRYIKKSALWVKGLKRN"
     gene            6779..8458
                     /locus_tag="VVL45_000798"
     CDS             6779..8458
                     /locus_tag="VVL45_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU4186999.1"
                     /translation="MDKLVAAIEKQQGKFEKISTNNYMMAIKDGFIATMPLIMFSSFL
                     MIIIMIPKNFGVELPSPAIVWMRKVYMLTMGVLGIIVSGTVGKSLVGNVNRKMPHGKV
                     INDISAMLAAICSYLVLTVTLVVDEKTGSTSLSTNYLGSQGLITSFVSAFITVNVYRF
                     CIKRDITIHLPKEVPGAISQAFRDIFPFSFVLLISGLLDIVSRFSLDVPFAQVFQQLL
                     TPIFKGAESYPAMMLIWFMCALLWFVGIHGPSIVLPAVTALQLSNMEENAQLLANGQF
                     PYHSLTPNFGNYIAAIGGTGATFVVPFILIFFMRSKQLKSVGKATITPVLFAVNEPLL
                     FGMPVILNPYLFVPFLMTPPVNVFLGKVFIDFFGMNGFYIQLPWTFPGPLGLLIGTNF
                     QLISFVFLSLILVVDILIYLPFCRAYDRQLLVKEDIASSNDIILEEDTSEIIPGEIDE
                     IKSKELKVLVLCAGSGTSAQLANAINEGAQLAEVRVIANSGAYGAHYDIMGVYDLIIL
                     APQVRSYYREMKVDAERLGIQIVATRGMEYIHLTKSPSKALQFVLEHYQAV"
     gene            complement(8584..10023)
                     /locus_tag="VVL45_000799"
     CDS             complement(8584..10023)
                     /locus_tag="VVL45_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEU4187000.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSVIDAF
                     FEISSGFTTTGASILNDISVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYLALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRIKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNQTVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(10027..11376)
                     /gene="trkA"
                     /locus_tag="VVL45_000800"
     CDS             complement(10027..11376)
                     /gene="trkA"
                     /locus_tag="VVL45_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEU4187001.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            11564..12412
                     /locus_tag="VVL45_000801"
     CDS             11564..12412
                     /locus_tag="VVL45_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEU4187002.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            12427..12975
                     /locus_tag="VVL45_000802"
     CDS             12427..12975
                     /locus_tag="VVL45_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEU4187003.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLAGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            12982..13239
                     /locus_tag="VVL45_000803"
     CDS             12982..13239
                     /locus_tag="VVL45_000803"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187004.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEIVDRTDEVSSKQCFEVVDETDEVS
                     NHTYGKAKLTWFEEIFEEYKMMGKAGQLVFFDVYRLVRQVS"
     gene            13341..15023
                     /locus_tag="VVL45_000804"
     CDS             13341..15023
                     /locus_tag="VVL45_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4187005.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQVAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLANLDNLSIS
                     KGQVQLQNELAKETPALQSLFRLEEVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            15008..15838
                     /locus_tag="VVL45_000805"
     CDS             15008..15838
                     /locus_tag="VVL45_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU4187006.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            16031..16534
                     /locus_tag="VVL45_000806"
     CDS             16031..16534
                     /locus_tag="VVL45_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEU4187007.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFMSQMKGKLYLISKFAEKIYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     regulatory      16588..16822
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            16987..17550
                     /locus_tag="VVL45_000807"
     CDS             16987..17550
                     /locus_tag="VVL45_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU4187008.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTVGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     gene            17564..18190
                     /locus_tag="VVL45_000808"
     CDS             17564..18190
                     /locus_tag="VVL45_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU4187009.1"
                     /translation="MKGSFALLLFVILGYMVKFYPETLVNFDQSIQTAIRGDLPDYLT
                     ILFRALTRLIDIPVIITWVVITAFVFYRKRWKIESFFMLGNLALAGLLIVTFKNIYQR
                     PRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKDPVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVLASLCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            18218..18607
                     /locus_tag="VVL45_000809"
     CDS             18218..18607
                     /locus_tag="VVL45_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEU4187010.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETIQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLEDRI
                     LAQDGYEAKWYNWCDGFNQAATLLLKR"
     gene            18612..19061
                     /locus_tag="VVL45_000810"
     CDS             18612..19061
                     /locus_tag="VVL45_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4187011.1"
                     /translation="MCFVPYYKVNYCEEAFAWYQDVNLVYLVDGVKLPYSQATLEAMY
                     SYLDRHGELFWIEVKEKGEWFPIGDITLSQDNLPIVIGNSAYQHRGLGKKILSALIEL
                     ARVKGWKELRVKEIYTYNHASRRCFKSLGFVENGATEKGRSFILELV"
     gene            19189..19776
                     /locus_tag="VVL45_000811"
     CDS             19189..19776
                     /locus_tag="VVL45_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEU4187012.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            20166..21773
                     /locus_tag="VVL45_000812"
     CDS             20166..21773
                     /locus_tag="VVL45_000812"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEU4187013.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDVEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
     gene            complement(22332..23963)
                     /locus_tag="VVL45_000813"
     CDS             complement(22332..23963)
                     /locus_tag="VVL45_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEU4187014.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGVGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
     gene            24256..29235
                     /locus_tag="VVL45_000814"
     CDS             24256..29235
                     /locus_tag="VVL45_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEU4187015.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHARENKLEKAEGVAIASETASPASNEAATTE
                     TAEAASAAKPEEKASEVVAETPSAEAKPKSDKETEAKPEATNQGDESKPAEEANKTEK
                     EVQPDVPKNTEKTLKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNE
                     KASKEAKVQALSNTNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDA
                     GHRNGVPVYGTLFFNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFI
                     NQETTGDLVKPLGEKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYN
                     YQFMQPEGDKVPADNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKT
                     KVKWNDILDENGKLRLSLGLFAPDTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGD
                     KDWYGIANLVADRTPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWR
                     WWQTSTGEKLRAEYDFTDAYNGGNSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVA
                     HKGGKGSKVYMAFSTTPDYKFDDADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFF
                     EHEGAVKDYQFNLGQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADF
                     YEVYEKDGDSWKLLTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTT
                     FDWGMTVKDTSLPKPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSA
                     QLSGSVDIRLTKSRTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEV
                     RGNKAHVTDITLDKPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMA
                     KAAARSLGNNKVQVGFADVPAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQ
                     SGLLYYRTQLPGKEISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLR
                     VQYEGGTEDELIRLTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEAS
                     PKTILGIEVSQEPKKDYLVGDSLDLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQK
                     TGRQTLTLRYQGHEVNFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASPEVKEA
                     FLKAIEAAEQVLKDHETSTQDQVNDRLNKLTEAHKALNGQEKFTEEKTELDRLTGEAQ
                     ELLAAKPNHPSGSALAPLLEKNKALVEKVDLSPEELATAKQSLKDLVALLKEDKSAVF
                     SDSKTGVEVHFSNKEKTVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVD
                     LSYASIVKIPIEKDKKVKKVFFLPEGKEAVELAFEQTDSHVIFTAPHFTHYAFVYESA
                     EKPQPAKPAPQNTVLPKPTYQPASDQQKAPKLEVQEEKVAFHRQEHENAEMLVGEQRV
                     IIQGRDGLLRHVFEVDENGQRRLRSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQ
                     NTAVKSEEASKQLPNTGTADANEALIAGLASLGLASLALTLRRKREDKD"
     gene            29325..30521
                     /locus_tag="VVL45_000815"
     CDS             29325..30521
                     /locus_tag="VVL45_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEU4187016.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            30657..31184
                     /locus_tag="VVL45_000816"
     CDS             30657..31184
                     /locus_tag="VVL45_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEU4187017.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETISYVFVRVSETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            31261..31617
                     /gene="glnR"
                     /locus_tag="VVL45_000817"
     CDS             31261..31617
                     /gene="glnR"
                     /locus_tag="VVL45_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HEU4187018.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            31654..33000
                     /gene="glnA"
                     /locus_tag="VVL45_000818"
     CDS             31654..33000
                     /gene="glnA"
                     /locus_tag="VVL45_000818"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEU4187019.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYVAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(33096..33215)
                     /locus_tag="VVL45_000819"
     CDS             complement(33096..33215)
                     /locus_tag="VVL45_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEU4187020.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
BASE COUNT        10297 a         5874 c         7378 g        10151 t
ORIGIN      
        1 tgtttttata ataataaaat gcagctagaa gaaaagttat tttaatataa atcactcaac
       61 atagcgagaa ggcggtttcc tatatggaag tcgtcttgtt ttcatattgt ctaaatacga
      121 gatatctact acaggttata gttgatttaa acaggtctat agatttctaa tatattaagg
      181 tgaaataata tcttgtaata cattgctatg ttctatgaaa aaagcgacga tagctaatgc
      241 acaagctcct atacttccac gatgtttgtt gtaaggtaaa atttcaattt ctatattacg
      301 agtaaaggga acgaagtgga gctggtcttg gattttatcc ctgacttcat gagtgaaagg
      361 ttgataatta agcaattgac tgttgatata gattttatca gcaggattga tgaggaggag
      421 atttgcaata gaagtggcta ataaattgac tcctttatca atttgttgac gtaaagcgga
      481 gtcgcctaaa ttataagccg ttaaaagggt gtctaaatga atgtcttttt cagtctttac
      541 aaggcttttt agtacagtta gttgggaatt tttaaataat aattgggcgt gtttgattaa
      601 ccaagcatcc gaaatatatg tttgtaaaca accttttttt ccgcattcac aatattgccc
      661 attcaattcg acaatggtgt gtccaatttc tccgatataa ggatttttag aggctcctat
      721 tttttctttt gtgaaaaagg aagtgtaaat acctaatcca gcgtgtagga aaataaagtt
      781 atcgggggta ttgtgtggat taaaaaggta ttgtcctata gccatacaat cgatattatt
      841 ttttacaata aaaggaaaat tgaatctttt aattacattt aataaattaa aagattccca
      901 tatggggttg tttgtaatga tacttccgga gtctttgtta taatgtccag gtattgataa
      961 gccaaatcca agtaattttg tttccaaagg gacgtactct atagctgttt ttatcatatt
     1021 ggtaatggtg gaattgattt ctgaaatagg ataatcttca ttcaaaattt ctgtctcgaa
     1081 aagagttttc ccttgtaagt cacatagact gagaaagata cctaggacgt ttatttcaat
     1141 acctccaaca acatacggaa tattatctgt gatagaaata gtttttctac gtcttccaga
     1201 tccggaaaac tcgctaaatt catcaccagt ttcgtatatg agggactgtt cgcttagctc
     1261 ttttatgatg ttagtcgttg ttgctggagt aagtcctgtt tcattaacaa tatctgtacg
     1321 ggatgttgct ggatttcgat atagaaactc cagtatagtg gctctatttt gtaaaaaggc
     1381 tgattgtttt gtaatcattt gtttcctcct ttggtttatt ttatattaaa cataagaaaa
     1441 aatcaattaa aattattatt ctatacaaag aaaaaaatat tgtaaaaaca acaaaaaata
     1501 tattgacaac gctttctgtt agtggtatat ttaatttata aaaaataaaa aaaggagttc
     1561 cggtgatgaa caatatttta gcgtttttag aaacaaaagt cgctccgttt ggtgaaaaag
     1621 ttggcaacca acggcatttg aaagctattc gtgaaggatt tatgatggca atgcctttga
     1681 ttttagtcgg ctctttattt cttattctaa tcagttggcc tcaagaggct tttacaaatt
     1741 ggctgaatag tgttggattg ctaagtatct tgacaactat gaatcagtca acagtagcga
     1801 ttatctcctt ggtcgcttgt ttcggtattg cctacaggtt gtcggaagga tatggtacag
     1861 atggtccgtc ggcagggatc atagccttat ccagttttgt attgatggca cctcgttttt
     1921 cgagtatggt ttatgataaa aatggggagc aggtcaagca gttatttggc ggcgcaatac
     1981 cattttctag cctgaatgca tcttctttgt ttatggcgat tactattgga ttggttacag
     2041 cagagattta tcgtatgttt atccagcgcg gaattacgat aaaaatgcca agtggtgtcc
     2101 cagatgtagt aagtaaatca ttttcagctc ttttatctgg ttttactact tttgttttgt
     2161 gggctttggt cttaaaaggt cttgaagcgg caggagttgc aggaggtctc aacggactcc
     2221 taggtgcaat tgttggaaca ccgcttaagt taattgcagg aacgcttcca ggtatgattc
     2281 tatgtgttat tgtaaactca ttcttttggt tctgtggagt taatggggga caagttttaa
     2341 atgcttttgt agacccagtt tggttacaat ttactacaga aaaccaagaa gctgtggctg
     2401 caggacaaac actccaacac attattacat taccgtttaa agatttattt gtatttattg
     2461 gtggcggtgg agcgactatt ggtcttgcga tttgtctctt cctatttagt aagagtcgtg
     2521 cgaataaaac attaggtaag ctagctatta taccgtctat ttttaatatc aatacagcta
     2581 ttctatttac gtttccaaca gttttaaatc cgattatgct gattccgttt attgctactc
     2641 ctacaatcaa tgccttgatt acctatgtat caatggctgt aggattagta ccctatacaa
     2701 caggtgtaat ccttccgtgg acaatgccac cgattatagg aggcttcctt gcaacagggg
     2761 ctagttggcg aggagctcta ttacaagttg ttttgatttt ggtttctgta gcaatttatt
     2821 atccattctt caaaattgca gataaacgca atcttgaaaa agaaaaagct actgttggag
     2881 ggaaataaga tggttatcag agtatttgat caacagaaaa atacttattc tagctttgcc
     2941 ttagaggaat taagttacta tatgaatcgg gtctttaaga ctaacataga gcttgtcgag
     3001 gagaaggaag cggatatttt tgtaggatta gtcaataaag aggacagaaa agaccatgtt
     3061 cttatctcat tagacaaggg taaggggaga attgagtcta atacaattgt aggtttactt
     3121 attggaattt accgaatgtt tcatgaattt ggggttgtgt atactagacc agggcgcaga
     3181 catgactttg ttccagagtt acgatttgaa gattttttag ataaacagct atctatagat
     3241 gaaacagcca gttactatca taggggagta tgtatagagg gagcggattc atttgaaaat
     3301 atactagatt tcattgattg gctacctaag attgggatga acagtttttt catccagttt
     3361 gaaaatcctt actctttttt gaaacgttgg tatgaacatg aatttaatcc atatctaaat
     3421 aaagaacaat tttcaaatga attagtacaa gaattgagtg ataggttgga taaagaattg
     3481 caaaaaagag gtcttattca tcatcgtgtt ggtcatggat ggacaggtga agttttaggt
     3541 tactcttcaa aatttggctg ggaatcaggt cttagtattt cagaggagaa gaaaccctat
     3601 gtcgctgaaa taaacgggaa acgagaattg tttaatacgg ctccgatttt aaccagcctg
     3661 gatttttcaa atccagatgt agctgataag atggtagaaa ttatcaagga ttatgccaag
     3721 aaaagacctg atgttaacta cttacatgta tggttgtcgg atgctcgtaa taatatttgt
     3781 gaatgcgaaa actgtagaca agaattggtt tcggatcagt atattcgtat tctcaatcaa
     3841 ttggataggg ctttaacgag tgagggatta gatacaaaga tttgttttct gctttatcat
     3901 gagttgttat gggcacctca gaaagaaaaa ttagataatc ctgaacgctt taccatgatg
     3961 tttgcaccga ttacaagaac atttgaaatg agttatgcag atgtagattt tgacaattcc
     4021 atacctacgc ctaaacctta tatgcgtaat aaaattatac ttccgaattc tcttgaggaa
     4081 aatttatctt atctttttga gtggcaaaaa gcatttaaag gagatagttt cgtatatgac
     4141 tatcctttag ggcgtgctca ttatggcgat ttaggctata tgaaaattag tcaaactatt
     4201 tacagagatg tatcttatct ttccaaccta catttgaacg ggtacatttc gtgtcaagaa
     4261 ttacgtgccg gattccctca taattttcct aattatgtca tgggggaaat gctctggaag
     4321 aagacaagaa gttatgaaga attgattgaa gaatactttt ctgctttgta tggggaaaat
     4381 tggcagtctg ttgttgaata tttagaaaaa ttatccattt attcctcttg tgattatttt
     4441 aatgcaattg gcagccgtca aagtgatgtt ttagcgaatc attattatat agcttacaat
     4501 ctagctgata attttttacc aattattgag gaaaatattt ctaagttatt aaatagtcaa
     4561 aaggatgaat ggaaacagct cagttatcat cgtgaatatg ttgttaagat ggcgaaggct
     4621 ttatatcttc aagcaactgg aaaaacaagg caagctcaag atgaatggag aaatgtgttg
     4681 aattatatcc gtgggcacga attgctattt caatctaatt tggatgttta tcgtgtaatt
     4741 gaagtagcaa aaaattacgc tggtttccac ttataaatca taagtataga aaatgaacta
     4801 aggtattcag agaagattga tcctaaatat tatgaaattt aaggattttt aagatattta
     4861 gggtcaactt tctatttata tcgtagcgaa gtcattttaa taatgatgtg taaaagatgg
     4921 atcaagattg aggaggaaga aagatgaaat caaaagaaga aataaatatg cttggtttta
     4981 caattgtcgc ttacgcagga gatgcaaggt cagatttgat ggatgctttg gcgtttgcga
     5041 gagatggata ttttgaacag gcaagagaat tggttgagtc tgcaaacgac tcaatagtgt
     5101 ctgcccatcg agaacagact aatttattag cggaggaggc atatggagat aattttgaag
     5161 tgagctttat tatgattcat ggtcaagata ctttgatgac aacgatgcta ttgtatgatc
     5221 aggtaaagtt ttttattgat gaatatgaac gaattcgaaa gattgaagaa catattggtt
     5281 tgcaatgagg attagtcatg gaaaatttac aggttaaagc cttaccgaag gagtttttat
     5341 taggaactgc taccgctgct tatcaagtag agggtgcaac tagggtagat ggcaaaggaa
     5401 taaatatgtg ggatgtttat ttgcaagaaa atagtccgtt cttaccagat ccagctagtg
     5461 atttttatta tcgttacgaa gaggatatag ctttggcggc agaacatggt ttgcaggctt
     5521 tgcgtttatc tatttcttgg gttcgtatat ttcctgatat agatggggat gctaatgtat
     5581 tagctgttca ttattaccat agagtttttc agtcttgctt aaaacataat gtgattccgt
     5641 ttgtttcttt acatcatttt gattcgcctc agaaaatgtt agaaacaggg gattggttga
     5701 acagagagaa tattgatcgt ttcatacgat atgctcgctt ttgtttccaa gaatttacag
     5761 aagtcaagca ttggtttaca atcaatgaac tgatgtctct tgctgcaggt caatatatag
     5821 gaggtcagtt tcctccaaat catcattttc aattatctga agcaattcaa gcgaatcata
     5881 atatgttgtt ggcgcatgct cttgcagtcc tcgaatttca tcaattaggg attgagggaa
     5941 aggtaggttg tattcatgct ttaaagccag gctatcctat tgatgggcaa aaagaaaata
     6001 ttttggcagc taaacggtat gatgtttata ataataaatt tctattagat ggaacttttt
     6061 tgggctacta cagtgaggac acgctttttc acttgaatca aatattggaa gctaataatt
     6121 ctagctttat tattgaagat ggtgatttag aaattatgaa gagagctgca cctcttaata
     6181 cgatgtttgg gatgaattat tatcgttcag aatttattcg tgaatacaaa ggtgaaaata
     6241 gacaagaatt taattcaaca ggaataaaag gacagtcttc ttttaaatta aatgctctag
     6301 gtgaatttgt aaaaaaacct ggtattccga caacagattg ggattggaat atttatcctc
     6361 aagggttatt tgatatgttg cttcgtatca aagaagaata tcctcaacat ccggtcattt
     6421 atttaactga aaatggtaca gcccttaaag aagttaagcc agagggcgag aatgatatta
     6481 ttgatgacag taagagaatc cgttatattg agcaacattt acacaaagtt ttagaggctc
     6541 gagatagagg agtcaatatt caaggctatt ttatatggtc tttgcaagat caattttctt
     6601 gggcgaatgg ctacaataag cgatatggtc ttttctttgt tgattatgaa acacagaaga
     6661 gatatattaa gaaaagtgct ctttgggtaa aagggctaaa acggaattaa ggttagcgat
     6721 ttgactgatg tttaatatgt tttaaatatg aggttgaatt ttttatagga ggagttttat
     6781 ggataagcta gtcgctgcca ttgaaaagca acaagggaaa tttgaaaaaa tttctactaa
     6841 taactatatg atggctatta aagatggatt cattgctact atgcctttaa ttatgttttc
     6901 aagctttttg atgattatta ttatgattcc taaaaatttc ggagtagagt taccgagtcc
     6961 agctattgtc tggatgagaa aagtgtatat gttaaccatg ggagttttgg gtattattgt
     7021 ttcagggact gttggaaagt cattagttgg aaatgttaac agaaaaatgc ctcacggaaa
     7081 ggtaataaat gatatttctg caatgttggc agccatatgt agttatctgg tattaactgt
     7141 aacgcttgta gttgatgaga agacgggatc tacaagtttg tcgacaaact atttaggatc
     7201 tcaaggattg ataacttcgt ttgtcagtgc ctttattact gtaaatgttt accgattctg
     7261 tattaagcga gacattacta ttcatttacc taaggaagtt cctggggcta tatcacaagc
     7321 ttttagagat attttccctt tttcttttgt tttacttatt agtggtttgt tagatattgt
     7381 atctcggttt agtttagatg ttccttttgc ccaagtattt caacaactat tgactcctat
     7441 ttttaagggg gcagaatcat atcctgctat gatgttgatt tggtttatgt gtgctttgct
     7501 ttggtttgtt ggaattcatg gaccatctat tgtcttacct gctgttacag ctttgcaact
     7561 gagcaatatg gaagagaatg ctcaacttct tgcaaatggg cagttccctt atcattcttt
     7621 aacacctaat ttcgggaatt atatcgctgc tattggagga acgggggcta cctttgttgt
     7681 accatttatt ttgattttct ttatgcggtc taaacaatta aaatcggtag gtaaagctac
     7741 aattactcct gttttatttg cggtaaatga acctcttcta tttggtatgc ctgttatttt
     7801 gaatccctat ctttttgtcc cttttttgat gactccacca gtgaatgtat ttctaggaaa
     7861 ggtctttatt gatttctttg gaatgaatgg attttatatc cagttacctt ggacctttcc
     7921 tggtcccttg ggattgttaa ttggaacgaa ttttcaactt atctcctttg tatttttatc
     7981 tttgatttta gttgtcgaca tattgattta tttgccattc tgtagagcgt atgatagaca
     8041 gttactggtg aaagaagata ttgcaagctc aaatgatatt attttagagg aggatacaag
     8101 tgaaataatt cctggtgaga tagatgaaat aaaaagtaag gagttgaaag tactggttct
     8161 ttgtgcaggg tctggaacaa gtgcgcaatt agccaatgca attaacgagg gcgctcaatt
     8221 agcagaagtt agagtgattg cgaattcagg agcgtacgga gctcattatg atattatggg
     8281 tgtttatgat ttaattattc tggccccaca agttcggagt tattatagag agatgaaggt
     8341 ggatgcagaa agattaggta ttcagatagt tgctaccaga ggaatggaat atattcattt
     8401 aacaaagagt ccaagtaaag ccttacaatt tgtattggag cattaccaag ctgtgtagta
     8461 agtttttcca tcttttattt gagtaaagat tttgcttaca gataggcttg gatttaaaaa
     8521 cgttccccct tttttaatat aagaatccct ctttcacaat tgtaaaaaga gggattttgt
     8581 attttatctc ttagaccaag ttctcttcat aaagagaagg aggattgggt aaatctccaa
     8641 gcgccctgca atcattgcaa aggataggag aatttttgag atgggactaa agattgagaa
     8701 actagaagtg gttcctagaa taggcccgat attattgaaa cagctaaaga cagcgctggt
     8761 cacgaccaga aaatcattgc tatctaggct gacaataaag ataagcgcta gcaaaatcat
     8821 agcatagatg acaaagtact tgagaatctt atgctgggta tctttgtcaa tcaccgtttg
     8881 attaacatgg agggtcaaaa cacggtgggg cgataggatt gacaaaattt ggtttttggc
     8941 aatttttgaa aggatgaggc ctcgaataat cttgagtcca cctgcagttg atccagcaga
     9001 gccaccgatt gccatgagga aaaggaggat aaactgggag aagaggggcc agttggtaat
     9061 atctccatat ccaaaaccag ttgttgtaat gatgttggaa acctggaaga aggtcatttc
     9121 aaagctcttt gaaaaccctg ggtagaggta gagggtgttg aggctaatca agcctgtaga
     9181 aaccagtaca atgaccaagt aagcccgaag ttcttcgtca ccaaagaagg ccttgatgcg
     9241 acggagcatg aggtagtagt agaggttgaa atttacccca aaaaccagaa ctccgatact
     9301 gaccagatag gtaatcagtg agctgccata gtgggcaatt ccgtcgttat agacggtaaa
     9361 gcctccagtt cccgctgtcc ccatagcaat gacaaaacta tcgtagagag gcataccagc
     9421 tagataatag atgatgacaa agagggagaa gagagctaga taaaggagat agagaatctg
     9481 ggcagtgttt tttagtttgg atacaacctt gccaaaaaca ggacctggaa cctcagcctt
     9541 catcacctct aggtggctat ttttggcatt gtccataata gcaagtgcaa aaacaagcac
     9601 tcccatccct ccaatcaagt gggtaaaact tcgccagaag aggagggaac ggctgagaac
     9661 cgaaatgtcg ttcaaaatac ttgctccagt agttgtaaac ccagaactaa tttcaaaaaa
     9721 ggcatcaata acgctaggga tttgcccaga aaagacaaag gggagaccac caaagaaaga
     9781 ccaaaggatc caacagaggg caacgatcaa gactccctcc ttggcataaa tccgttgatt
     9841 ttttggcttc tgtaaactcc ctgaaccgcc taacaatacg agaatcccta tggtcgaaaa
     9901 gagggctgta aagacttggc tcgattcacg gtaatagaca gcgatagcca caggaaccaa
     9961 gaggagaaca gcttcaatca aaagtaactt tgaaaggagg taacgaatca tacttttatt
    10021 catttcttac ctcgcaatca agtcataaat cttggtgatg tttggcaaca aggttgttac
    10081 taggagcttg tctccaactt ccaacatatc ctccccagtt gggaaaatag tcttgccctt
    10141 tcgaataatg gctgcaataa gaaccccttt tttcaatttc agttgagaaa gaggtttggc
    10201 agtcatttta ttggcttcct tgatatggaa ttgcagggtt tcaatttggc cattggctag
    10261 atggtgcatg gcttgaaggt ctgaatactg ggcattaact cgaccacgaa taaagtgcat
    10321 aatcgtatct acagcgatgc ttttaggtgt gatgatactt gaaaaatcag gcgcattgat
    10381 aatctcgagg agactggtac gattgacctt agtaatattt ttctgtacac ctaccctgtc
    10441 aaggaacata gatgtaatca gattttcctc atcgacacct gttagagtcg caacggcatc
    10501 atagtgttga gcactttctt ccagcaggat atcttttgcg gttccatctc cttgaacgat
    10561 gtagagattt gggaatttct cgctaaagaa gctggcgatt tcaggattga tttcaatgac
    10621 ttttgtatcg atacgactat ctttgagaat accaagtaga taataggcaa ttctacctgc
    10681 cccaacgatg agaaggctct tcacggcgcg tgatttaaaa taattatgga agagtatcat
    10741 atcgacacgg ttaccagtga caaagattct atctttatcc tgtacagtca tgtcaccgct
    10801 tggaatgata atttgatgat ccctctctat cgcacagaca atgacattac caaatttttt
    10861 acgaaaatca gaaatgggca tttggcaaag accgctggtg gacttgacga caaattccat
    10921 gaggctaacg cgtccaccag caaagcgttc gacagacagg gcgttgggga agtcaatgat
    10981 attcgcgata gcgcgggcag ccaagagctc aggattaacg ataagagaaa aaccgagaat
    11041 attcttttcc ttgaaataag agttagaata ttcagggttc cgcacccgaa cgatagtttc
    11101 tttagctccc attttcttgg ctagaactgc tgcaatcatg ttgacttcat cgtgctcagt
    11161 cagggcgata aagatatcac aatcttggac gctggcttgc tcaagaatgg caaaatcggc
    11221 cccgttacca aggataccaa tgatatcaaa gcgactgaca atatgattga gaacagcttc
    11281 gtcttgctca atcagcaaaa catcatgctt ttctgcaacc aaggagcgac agagggcaaa
    11341 accaactttt ccccctccga caaggataat tttcataata aaacctactt tttcatgatg
    11401 taactatcat accctttttc aagaaaaaat gcacctacta gctaataaca agagctttta
    11461 gtgaaaattc gctataaggt aaaactatac cctaaccaat tgaaatagct attagcgact
    11521 ttctctgaaa tatggtatga taaaggatat acaaggagat aaaatgaata ataatttact
    11581 ggtattacaa tcagactttg gtctggttga tggtgcggta tcggctatga ttggagtggc
    11641 tttagaagag tctccaacct taaaaatcca tcacttgacg cacgatatca cgccttataa
    11701 tatttttgag gggagctatc gtctctttca gacggtggat tactggcctg agggaacgac
    11761 gtttgtatcg gttgtcgatc caggtgtcgg ttcgaaacgt aagagtgtag ttgccaagac
    11821 tgcaaaaaat caatacattg tcacgccaga taatgggacg ctttccttta tcaagaaaca
    11881 cgttggcatt gtagccattc gtgagatttc tgaggtggcc aataggcgtc aaaacacaga
    11941 gcattcttat accttccacg gtcgtgatgt ctatgcctat actggtgcta aactggccag
    12001 tggtcacatt acttttgagg aagtagggcc agagctcagt gtggaacaga ttgtagagct
    12061 tccagtcgta gcgaccatca tagaagatca tctggtgaag ggagccattg atattctgga
    12121 tgtgcgtttc ggttcgcttt ggacctctat cacacgggaa gaattttaca agctggaacc
    12181 agaatttggt gatcgttttg aagtgaccat ctatcatgct gatatgctgg tctatcaaaa
    12241 tcaggttgtc tatggcaaat catttgcaga tgtgagaatt gggcaaccca tcctttacat
    12301 caactctctc tatcgtttag gtttggctat caaccaaggt tcctttgcca aggcctataa
    12361 tgtaggtgtc ggttcatctt ggaccattga aataaagaaa atagaaggat aaaataggag
    12421 aatggtatgg aaatcaaatt tacaattaaa caagttgttg ctgtcggaat tggcgctgcc
    12481 ctctttgtcg tcatcgggat gatcaacatt ccaacccctg ttccaaatac aagcatccag
    12541 cttcagtatg cggtacaagc gctactttct attatttttg gaccgattat cggtttgctt
    12601 gtcgggttga ttggtcatgc aatcaaggac tctcttgctg gttatggtct atggtggact
    12661 tggattatcg ctagcggact ctttggtcta gttgtgggac tttttagaaa gtacgttcga
    12721 gtgatcaatg gtgtttttga ctggaaagat attcttattt ttaacctcat tcaactactt
    12781 gcaaatgccc ttgtttgggg tgtcttggca ccacttggag atgttgtgat ttatcaagaa
    12841 gcggcagaaa aagtatttgc acaagggatt gttgcgggaa ttgccaatgg tgtatctgta
    12901 gctattgcag gaactcttct cttacttgcc tatgcaggaa cccaaactcg tgcaggaagt
    12961 ttgaaaaagg actaatactc aatgaaaatc aaagagcaaa ctaggaagct agccgcaggt
    13021 tgctcaaaac agtgttttga gattgtggat agaactgacg aagtcagctc aaaacagtgt
    13081 tttgaggttg tagatgaaac tgacgaagtc agtaaccata cctacggcaa ggcgaagctg
    13141 acgtggtttg aagagatttt cgaagagtat aaaatgatgg ggaaggctgg gcaactagtc
    13201 tttttcgatg tttatagatt agttaggcaa gtaagctagg ataggaatct ttttggcgct
    13261 aagattttaa gagaagagac tgcaagaagg gcttgattta tgataaaata gaagtctaag
    13321 aagcgaatga agagagtaag atgaaagaag ctataattga gtggaaggat ttctctttcc
    13381 ggtatgaaac acaacaagaa ccgaccttgc aagggataga cttgaccatt tacaagggag
    13441 aaaaagtctt aattgttgga ccatctgggt caggtaaatc taccttgggt cagtgtttga
    13501 atgggattat tcccaatatt tacaagggtc agacatatgg agaatttttg ataaagggtc
    13561 aagtagcctt tgatatgagc atctatgata agtctcatct ggttagcaca gttttgcagg
    13621 atacagatgg gcagtttatt ggcttgtctg tggcagaaga tttggcgttt gctctggaaa
    13681 atgatgtgac agccctagat gagatgaaag gtcgtgttta taaatgggct gaaaagctgg
    13741 accttcttcc tttactggat cagcgtcctc aggatttgtc aggtggacaa aagcagcgag
    13801 tcagtctggc tggtgtcttg attgatgaaa gtccgattct cttgtttgat gagccactcg
    13861 ccaatctaga tcccaagtca ggtcaggata ttatcgaatt gattgaccag attcataagg
    13921 aagaggggac gacgactctg attatcgagc accgtttgga ggatgttctg catcgccctg
    13981 tggatcggat tgtcttgata aacgatggtc gtatcctttt taatgggagc cctgaccagt
    14041 tgcttgcgac tgatttattg actcaaaatg gaattcgaga acccctttat ctaacgactc
    14101 tccgtcaatt aggtgtggac ttagtcaagg aagaacaatt agcgaatctg gataacttgt
    14161 ctatctcaaa aggtcaggtt cagttgcaga atgaactggc aaaagaaacc ccagcattgc
    14221 agtcactctt tagactagag gaagtatctt tttcttatga tgatagaccg attttaaaat
    14281 ccctacattt agatattaaa aagggtgaaa agattgctat tgtcggaaaa aatggagcag
    14341 ggaaatcaac tctagccaag gctataagta gctttattca gacggaagga cgctatcttt
    14401 gggaaaaaca ggatataaaa ggcgattctg ttgcagagcg ggcggaacga gtaggctatg
    14461 tgctacaaaa tcctaatcaa atgatttcaa ccaatatgat ttttgatgag gtggctctag
    14521 ggctccgttt gcgaggtgtg gatgagaagg aaattgaaac gagagtctat gaaaccttga
    14581 aaatctgtgg actttatgaa ttccgtaatt ggcctatttc tgccctgtca tttggtcaga
    14641 aaaaacgtgt caccattgct tcaattttgg tcttaggagc tgaaattatt ctcctagatg
    14701 aaccgactgc aggtcaagat cagaagaact atactgagat tatggaattt ctcgaagagt
    14761 tacatcaaaa agggcatacc attgtcatga ttacccatga tatgcaattg atgctggatt
    14821 attcagaccg ggtccttgtc atggtggatg gagaattgat tgccgatact gttccagcca
    14881 gtctgttgag cgatcctgag ctgttagtaa aagccaatct aaaagaaacc tccatcttta
    14941 acttggctaa gaaactagat gtggatccac tggatttaac ggcattttac aaagaaagga
    15001 gagagggatg caagctaaat taatcggtta ccagcataga gatactgtga ttcatcgctt
    15061 gtcaggagct gggaaacttc tctttttcat tctggtatca ttggcggcca tgattagcta
    15121 tgataccaga atgctcttgc tgattgccat cttttcagtc tttctcctct atttatcaga
    15181 aattcgcttt aaagatgttt cctttgtagc cgtttttgcg acggtatttg ccgttttaaa
    15241 cgttttgatg gtctatctct tttctcccga gtatggggtt ggactttatg gagagagaag
    15301 tgtgatttgg cagggaatcg gtgtctacac tctaaccagc caggagctct tttatctgct
    15361 aaatttggcc attaagtatc tttgcaccat tcctctggct attatctttt tgatgacaac
    15421 ccatcctagt cagtttgctt ccagtttaaa tcaaattggt gtgccctata agattgctta
    15481 ttctgtcagc ctgaccttgc gctatattcc agatttgcag gaagaattct ttactatcaa
    15541 gatgtctcag gaggcgcgtg ggatggaatt atccaagaaa gcttctctta tgcaacgaat
    15601 caaaggcaat ctgctcatta ttacgccctt gatttttagc tcgctagaac gcattgatac
    15661 cattgcgact gccatggagc ttcgccgctt tgggaaagag aaaaaacgca catggtatag
    15721 ttatcaggcc ttgaaaaaag gagactatat taccttgctc ttggcaacct tgtttttagt
    15781 agttagttta ctacttatct tgcagaatca gggacgattt tacaaccctt ggaaatagct
    15841 tgaaaaaatt gaaaaaatca agtcatttct attgacaatg attctgaaag tgttatacta
    15901 agaaagtagt ttcgctgatt tacttcaaac ctgttgtgag gtaagttaac gatgccttaa
    15961 ccacgctgtt tgctgagctt gactccgggc agtgtggcta tttttttgca atggtgaaag
    16021 gaagcaagtc atgacaaatc acattgtatt atttgaacct caaattccac aaaatacagg
    16081 caatatcgcg cgtacttgcg ctgcgaccaa ttctcccctc cacatcatca agcctatggg
    16141 ctttcctatt gatgaccgca agatgaagcg ggctggattg gattactggg ataagctaga
    16201 gatttatttt tacgagagct tagaagattt catgtctcag atgaagggaa aactctatct
    16261 gatttcgaaa ttcgcggaaa aaatctattc tgaggtggat ttatcgactg acgaggacca
    16321 ttattttctc tttggacgtg aagacaaggg cttacctgaa gattttatgc gagaacatcc
    16381 tgagaaggct cttcgtattc ccatgaatga tgaacatgtc cgcagtctca atgtgtctaa
    16441 taccgtctgc atgattgtct atgaggctct tcgccagcag aactttgcag gtcttgaact
    16501 cgtccatacc tatgaagtgg ataaattgaa ataaaaaatg aaaatgaaca aaatgcttgc
    16561 gcttgcaagc gttttttgtt atgataaaag agtcttcagg gcagggtgtg attcccgacc
    16621 ggcggtgact ttaactagga aatgatcttt tccttttata ctttgttgac aagctttgcc
    16681 taaccagaag ttatgcctac agcttgtcgc ctagtctaaa agaaaaatct ctatttcctt
    16741 ctctttaaag aagtccgcga gcgcaagctg atgtggtgag attccacaac cgacagtata
    16801 gtctggatgg gagaagacga aagaatagct ttgtctgttt tgatagattt atagccggat
    16861 tgtaaccact tgctttgttt ttctttaata gagtaagagg gaacttttgg gatataaaaa
    16921 gtgagaatag atagaggaat cctttccaac ttcttctgat tttatagaaa attggaggaa
    16981 cctgttatga caaacacacg tcgactttcg accattgcaa ttctatcagc catctcattt
    17041 gtgctgatgt actttgactt tccgctttta ccagcggcat ctttcctcaa gatcgaattt
    17101 agtatcttgc cagtccttgt gggtctggtg gtcatggatt tgcctgctgc tctaggagtg
    17161 ctcttgcttc gctcactatt gaaattgctt cttaacagcc aaggagtgaa tacttacatt
    17221 ggtttgccga tgaatatcgt agctttggga gtttttgtca tcgtatttgc tttgatttgg
    17281 aaaaaggaac ggacaaccct tcgtttccta ctaggctctc tagctggaac tgttggttta
    17341 accttggcca tgttggttct caactatgtt tatgctgttc ctttgtacgc taagtttgct
    17401 aactttgata ttggaaaaat tttgggactt tccaactacc taatgaccat ggtattacct
    17461 tttaacttga ttgaaggtgt tatcttttcc gtttcattct ggttgttgta tgttctcttg
    17521 aaaccaacct taaaacatta tgagagataa acaaacattt ttaatgaagg gcagttttgc
    17581 ccttttactt ttcgttattc ttggctacat ggtcaaattt taccctgaaa cgctggtcaa
    17641 ttttgaccaa tcgattcaga ctgccattcg aggagactta ccagattatt tgactattct
    17701 ttttcgagcc ctcacacgtc tgattgatat cccagtgatt atcacttggg ttgtcattac
    17761 agcttttgtc ttttatcgta agcgatggaa gatagaaagt ttcttcatgc tgggaaatct
    17821 ggctttggca ggtcttttaa tcgtgacctt taaaaatatc taccagcgcc cacgaccagc
    17881 tatcttacat ctggtggaag agaagggatt ttccttccca agtgggcatt ctctggctgt
    17941 aactttgatg gtagggactt tgattgttat tcttagtcag cggattaaag atccagtctg
    18001 gagaaaaatc gtacaaatcg tccttggcct ctacctagtc agtgtgctgg tatcaagagt
    18061 ctatctggga gttcattacc catcagacgt ccttgccagt ctctgtgtgg gcttgggagt
    18121 cctgtttatc gagtttccct tctatgacaa gctccgcttc caatggcgat ttaaaggcaa
    18181 acagaagtga gtatcgaatt cccttgagga gaaagaaatg aaagtcaata tagcagatct
    18241 tcatccgact caactatatt tatcagaaaa gaagttgcaa gatattcaga tgctttacca
    18301 gtcggcagaa acaatccaag tcgatccaat cagtattctt gcctttggag attgtttgct
    18361 gattacagat gggcatcaca gggcttatca ggctttattg gcaggtcggg atacgatttc
    18421 tgctgagtgg gatagagatg gtggtgatga actatatcat ctctatgcgc aagcttgcga
    18481 ggaaagaaag atttactctg tttttgattt agaagatcgt atcctagctc aagatgggta
    18541 tgaagcaaaa tggtataact ggtgtgatgg ttttaatcaa gcagcaactc ttttgttgaa
    18601 aaggtagtgc attgtgtttt gttccttatt ataaggtcaa ttattgtgag gaagcttttg
    18661 cttggtatca ggatgtgaac ttggtttacc tcgtagatgg ggtgaagctt ccttacagtc
    18721 aagcgacctt ggaagccatg tattcctatt tggatcggca tggtgagctt ttttggattg
    18781 aagtcaagga gaagggagaa tggtttccaa ttggggatat tacactatct caggataatc
    18841 tccccattgt gattgggaat tccgcttacc aacatcgagg acttggaaaa aagattctaa
    18901 gtgctttgat tgaattggct cgagtaaaag gatggaaaga attgagagtc aaggaaatct
    18961 acacctacaa tcatgcttct aggaggtgtt tcaagtcgct tggatttgtg gaaaatggag
    19021 caacagaaaa aggaaggagt tttatattgg aattagtcta atcctgccac tttctccccc
    19081 ttaacctttg actattccgc aaaattatcg taaaataaag agtaaatgat aaaatgaggt
    19141 cagagtctgt tcgctctggc gatagtagta taaatgagga gaaacgcttt ggaattagaa
    19201 gtatttgctg ggcaagaaaa aagtgaacta tctatgattg aggtagcgcg tgctatcttg
    19261 gaacttcgtg gtcgcgatca tgagatgcat tttagcgatc ttgtaaacga aattcaaaac
    19321 taccttggaa catcaaacag cgatatccgc gaagctttgc ctttgttcta cacagagttg
    19381 aactttgacg gtagcttcat ctcacttggg gacaacaaat ggggtcttcg ttcatggtat
    19441 ggtgtggacg aaatcgacga agaaatcatc gctcttgaag aaaatgacga cgatgaagta
    19501 gcaccaaaag ctaagaaaaa acgtgtcaat gcctttatgg atggtgattc agatgccatt
    19561 gactacaatg cagatgatcc agaagacgaa gatgcatacg aagcagatcc agctctttca
    19621 tacgatgatg aaaatccaga tgatgaaaaa aatgaagtgg aagcttatga tgcagaaatc
    19681 aacgaaatcg ctccagatga cttgggagaa gatgtggatc tcaacgaaga cgacgacgag
    19741 ttttcagatg acgacgctga aaccagcgag gaataagagt tagctattga caattatcct
    19801 atttttaggt atcatattgt ttgggcacct cttttagagg tcggggctcc ctagttctta
    19861 gggagctatt tttgtttttt caagaagtta tcttcttgta ttttatactc aatgaaaatc
    19921 aaagtgcaag ctaggaaact agccgtaggc tgctcaaaac actgttttga ggttgtagat
    19981 aagactgaca aagtcaggaa cacatatcta cggcaaggcg acgttgacgc ggtttgaaga
    20041 gattttcgaa gagtattagt tgtgaatctg gtgcagtcgt cccagattat tcttattagt
    20101 agggtcttgt tttctatatc ccctcgtagt taacaagacc ttgagcattt tagaaagagg
    20161 aatctatgtc tacgaaatat atttttgtaa ctggtggtgt ggtatcgtct attgggaaag
    20221 ggattgtggc agcgagtcta ggccgtctct tgaaaaatcg tggtctcaaa gtaaccattc
    20281 aaaagtttga cccttatatc aatattgatc cgggaaccat gagtccttac cagcacgggg
    20341 aagtttttgt gacagatgac ggagctgaga cagatttgga cttgggtcac tatgaacgtt
    20401 tcatcgatat caatctcaac aaatattcca acgtgacaac tgggaaaatt tacagtgaag
    20461 ttcttcgtaa agaacgccgt ggagaatacc ttggggcaac tgttcaagtc attcctcata
    20521 tcacagatgc tttgaaagaa aaaatcaagc gtgccgctct aacgaccgac tctgatgtca
    20581 ttatcacaga ggttggtgga acagtaggag atatcgagtc cttgccattc ctagaggctc
    20641 ttcgtcagat gaaggcagat gtgggtgcgg ataatgtcat gtatatccat acaaccttgc
    20701 ttccttacct caaggctgct ggtgaaatga aaaccaaacc aacccaacac tctgtcaaag
    20761 aattgcgtgg cttgggaatc caaccaaata tgttggttat tcgtacagaa gagccagctg
    20821 gtcaaggaat taaaaataaa ctggcccagt tctgtgatgt ggcaccagaa gccgttatcg
    20881 aatcgttgga tgttgaacac ctttaccaaa ttccactgaa cttgcaggca caagggatgg
    20941 accaaattgt ttgtgatcat ttgaaattag acgcaccagc agcggatatg acagaatggt
    21001 cagccatggt ggacaaggtc atgaacctca agaaacaagt taagatttcc cttgttggta
    21061 agtatgtgga gttgcaagat gcctatatct cagtggtcga agccttgaaa cactctggct
    21121 atgtcaatga tgtagaagtt aaaatcaatt gggtcaatgc caatgatgtg acagcagaga
    21181 atgtagcaga actcttgtct gatgcggacg ggatcatcgt accaggtggt tttggtcaac
    21241 gtggtacaga agggaaaatc caagccatcc gctatgcgcg tgaaaatgat gttccaatgt
    21301 tgggagtctg cttgggaatg cagttgacat gtatcgagtt tgctcgtcac gttttaggtc
    21361 ttgaaggtgc caattctgca gagcttgcac cagaaacaaa ataccctatc attgatatca
    21421 tgcgtgatca gattgatatt gaggatatgg gtggaaccct tcgtttggga ctttatccgt
    21481 ctaagttgaa acgtggctct aaggctgctg ctgcttatca caatcaagaa gtggtgcaac
    21541 gccgtcaccg tcaccgttat gagtttaata atgccttccg tgagcagttt gaggcagcag
    21601 gttttgtctt ttcaggagtt tctccagaca atcgtttggt agaaatcgtg gaaattcctg
    21661 aaaataaatt ctttgtagct tgtcagtatc accctgaact gtcaagccgt ccaaaccgac
    21721 cagaagaact ctacactgcc tttgttactg cagcggttga gaacagcaat tagcaaaatc
    21781 agaacctttg agaaaaatct cagaggtttt ttgcatacga tgatattgca gtatatctga
    21841 ggtaggagtc ctctgtatgt acctgctacc gttgaaatca atagcgactc cctcttgccc
    21901 tgtgctagtg aatggattta tcagtatatt gaaatgaaat aaaatttgaa caaattaatt
    21961 cggaaagcca aatcaatttc tagcaaagtt ttaggaactg gattgtatag tgaattgaaa
    22021 taagatgtga acatctctat caggaaagtc aaattaattt atagaaatat tttagcagtc
    22081 aagatggact gttatagatt caatatacta tactttttta atttaatcca ctataataaa
    22141 atgaaataat aacaggacaa atcgttcagg acagtcaaat cgatttctaa caatgtttta
    22201 gaaatagagg tgtactattc tagtttcaat atattatata tctgcagaat actctttcag
    22261 tttcattcag cctagtcttg cttaggaaaa agtaagaagc catccaatta tgatggatag
    22321 cttcttggtt attagatttg ttctgcaaaa accttttcag caaggttcat agcgtggtct
    22381 gatacacgag tgtagtgtga gatgatgtcg ataaagttga ctccagcttg tgttgaacat
    22441 tcgcctttgt tgaggcgttt gatgtgggtt tttctgagaa cacgttctat cttattgatt
    22501 gcttcatgac gttctacaag actgcgtgct ttttcaatat cattgttttc cacactatcc
    22561 agagcatctt tgataaagtc actagtttgg cggtaaactt cctctaattc tttcaaggcg
    22621 gcatcagaaa attcaacatt tttccgttga agatagtcag tcagattgag tagagcctcc
    22681 gtgtggtctc caatccgttc caaatcacgg gaggaatcaa ggatattggt aagcacttca
    22741 ctttcttttt ggctgagagc ttcgcttgaa agggcaatga gataacgtgt taattgctca
    22801 tcgatggtgt taattgcttc ttcggtttta tgcccttttt cagcaacttt ttcatccaag
    22861 tcaatgatgt acttatagga aaggtcaaag gctttagcag cgtagtttcc taagtgcaag
    22921 agctctttct tagcatttcc tagagcgata gatggggcct gtttgatgaa atgttcatca
    22981 agatataagg gttcgtattt gacaacctcg tcctctccag gaataatctt ggttacaaag
    23041 taagccagag ctccgataaa tggaaattgg acaatggtgt tggtaatatt aaaggttccg
    23101 tgagcaaagg cgatggtcat ttccggtgct agatttagcg tagcttcaaa ccaatgaatc
    23161 aggacagtaa aaggaactag aaaaataacg cagacgactg ttccgataac gttgaaggca
    23221 acatgagctc ctgctacccg tttagctgca atattagccc ctaaagaggc aatgatggct
    23281 gtaatggttg tcccgatatt gtcaccaaat agaactggca aagctccctg tagatcaatt
    23341 agattgccgg cgtagaggtt ttgtaaaatc ccaatggtag ccgaagaagc ttgaattagc
    23401 aaggtcaagc cagtaccgac aaagacaccc aaaacaggat ttttacttag ctcaatcata
    23461 tagtccttaa agacctgtaa atccttgagt ggagccattg cgccgctcat gagattgagg
    23521 gcaaaaaaga taccaccgac accaaagagg atgcgtccga tattattgac tgtccgattt
    23581 tttgtaaaaa agagacagac ggcaccgata aagagcatag gtagggcata gttacctaat
    23641 ttaaaaccga tgagaaagga tgtgacagtt gtcccaatat tagcacccat gacaatcccg
    23701 atagcctgac gtaaggttaa gagaccggca ctgaccaggc cgactgtgat aactgttaca
    23761 ccagaactag actgaattag agcagtcatc ccaataccaa ccagaactcc aaagaaagga
    23821 ttactagtat atttgtcaat gtaaaaacga aggcgatctc cagcagcttg ttgtaaaccg
    23881 tctcccatgg tcttgatgct atataagaat agccccagac cacctaaaaa gtgaaataaa
    23941 atttcctgcc aattaatgga catttctttt tcctccgaaa aataatcgcg gaatatctcc
    24001 tattctattt taaaggataa aagtaaatct aacaagtgtt aatgtcaagt ttgtaaaaga
    24061 aaattataat tgataagtgt attattttta agagttttta accttatttt agatttgtgc
    24121 aaacagttat tgttttttgt ctaaaaatgt tttaggataa aaatgtgatt atcatggttc
    24181 tagagtttga taaaaaacca cagaatataa agcgctttct tgaaaacaac aaaatcaatc
    24241 ttttaggagg agaaaatgaa gaatccattt tttgaaagac gttgtcgtta cagtattcgt
    24301 aagttatcag taggagcctg ctcgctgatg attggtgctg ttttatttgc tggtccagcc
    24361 ttggctgaag aaactgcagt tcctgaaaat agcggagcta atacagagct tgtttcagga
    24421 gagagtgagc attcgaccaa tgaagctgat aagcagaatg aaggggaaca tgctagagaa
    24481 aacaagctag aaaaggcaga aggagtagcg atagcatctg aaactgcttc gccagcaagc
    24541 aatgaagctg caactactga aactgcagaa gcagctagcg cagctaaacc agaggaaaaa
    24601 gcaagtgagg tggttgcaga aacaccatct gcagaagcaa aacctaagtc tgacaaggaa
    24661 acagaagcaa agcccgaagc aactaaccaa ggggatgagt ctaaaccagc agaagaagct
    24721 aataagactg aaaaagaagt ccagccagat gtccctaaaa atacagaaaa aacattaaaa
    24781 ccaaaggaaa tcaaatttaa ttcttgggaa gaattgttaa aatgggaacc aggtgctcgt
    24841 gaagatgatg ctattaaccg cggatctgtt gtcctcgctt cacgtcggac aggtcattta
    24901 gtcaatgaaa aagctagcaa ggaagcaaaa gttcaagcct tatcaaacac taattctaaa
    24961 gcaaaagacc atgcttctgt tggtggagaa gagttcaagg cctatgcttt tgactattgg
    25021 caatatctag attcaatggt cttctgggaa ggtctcgtac caactcctga cgttattgat
    25081 gcaggtcacc gtaacggggt tcctgtatac ggtacactct tcttcaactg gtctaatagt
    25141 attgcagatc aagaaagatt tgctgaagct ttgaagcaag acgcagatgg tagcttccca
    25201 attgcccgta aattggtaga catggccaag tattatggct atgatggcta tttcatcaac
    25261 caagaaacaa ctggagattt ggttaaacct cttggagaaa agatgcgcca gtttatgctc
    25321 tatagcaagg aatatgctgc taaggtaaac catccaatca agtattcttg gtacgatgcc
    25381 atgacctata actatggacg ttaccatcaa gatggtttgg gagaatacaa ctaccaattc
    25441 atgcaaccag aaggagataa ggttccggca gataacttct ttgctaactt taactgggat
    25501 aaggctaaaa atgattacac tattgcaact gccaactgga ttggtcgtaa tccttatgat
    25561 gtatttgcag gtttggaatt gcaacagggt ggttcctaca agacaaaggt taagtggaat
    25621 gacattttag acgaaaatgg gaaattgcgc ctttctcttg gtttatttgc cccagatacc
    25681 attacaagtt taggaaaaac tggtgaagat tatcataaaa atgaagatat cttctttaca
    25741 ggttatcaag gagaccctac tggccaaaaa ccaggtgaca aagattggta tggtattgct
    25801 aacctagttg cggaccgtac gccagcggta ggtaatactt ttactacttc ttttaataca
    25861 ggtcatggta aaaaatggtt cgtagatggt aaggtttcta aggattctga gtggaattat
    25921 cgttcagtat caggtgttct tccaacatgg cgctggtggc agacttcaac aggggaaaaa
    25981 cttcgtgcag aatatgattt tacagatgcc tataatggcg gaaattccct taaattctct
    26041 ggtgatgtag ccggtaagac agatcaggat gtgagacttt attctactaa gttagaagta
    26101 actgagaaga ccaaacttcg tgttgcccac aagggaggaa aaggttctaa agtttatatg
    26161 gcattctcta caactccaga ctacaaattc gatgatgcag atgcatggaa agagctaacc
    26221 ctttctgaca actggacaaa tgaagaattt gatctcagct cactagcagg taaaaccatc
    26281 tatgcagtca aactattttt cgagcatgaa ggtgctgtaa aagattatca gttcaacctc
    26341 ggacaattaa ctatctcgga caatcaccaa gagccacaat cgccgacaag cttttctgta
    26401 gtgaaacaat ctcttaaaaa tgcccaagaa gcggaagcag ttgtgcaatt taaaggcaac
    26461 aaggatgcag atttctatga agtttatgaa aaagatggag acagctggaa attactaact
    26521 ggctcatctt ctacaactat ttatctacca aaagttagcc gctcagcaag tgctcagggt
    26581 acaactcaag aactgaaggt tgtagcagtc ggtaaaaatg gagttcgttc agaagctgca
    26641 accacaacct ttgattgggg tatgactgta aaagatacca gcctaccaaa accactagct
    26701 gaaaatatcg ttccaggtgc aacagttatt gatagtactt tccctaagac tgaaggtgga
    26761 gaaggtattg aaggtatgtt gaacggtacc attactagct tgtcagataa atggtcttca
    26821 gctcagttga gtggtagtgt ggatattcgt ttgaccaagt cacgtaccgt tgttagatgg
    26881 gtcatggatc atgcaggagc tggtggtgag tctgttaacg atggcttgat gaacactaaa
    26941 gactttgacc tttattataa agatgcagat ggtgagtgga agctagctaa ggaagtccgt
    27001 ggtaacaaag cacacgtgac agatatcact cttgataaac caatcactgc tcaggactgg
    27061 cgcttgaatg ttgtcacttc tgacaatgga actccatgga aggctattcg tatctataac
    27121 tggaaaatgt atgaaaagct tgatactgag agtgtcaata ttccgatggc caaggctgca
    27181 gcccgttctc taggcaataa caaggtacaa gttggctttg cagatgtacc ggctggagca
    27241 actattaccg tttatgataa tccaaattct caaactccgc tcgcaacctt gaagagcgaa
    27301 gttggaggag acctagcaag tgcaccattg gatttgacaa atcaatctgg tcttctttat
    27361 tatcgtaccc agttgccagg caaggaaatt agtaatgtcc tagcagtttc cgttccaaaa
    27421 gatgacagaa gaatcaagtc agtcagccta gaaacaggac ctaagaaaac aagctacgcc
    27481 gaaggggagg atttggacct tagaggtggt gttcttcgag ttcagtatga aggaggaact
    27541 gaggacgaac tcattcgcct aactcacgca ggtgtatcag tatcaggttt tgatacgcat
    27601 cataagggag aacagaatct tactctccaa tatttgggac aaccagtaaa tgctaatttg
    27661 tcagtgactg tcactggcca agatgaagca agtccgaaaa ctattttggg aattgaagta
    27721 agtcaggaac cgaaaaaaga ttacctagtt ggtgatagct tagacttgtc tgaaggacgc
    27781 tttgcagtgg cttatagcaa tgacaccatg gaagaacatt cctttactga tgagggagtt
    27841 gaaatttctg gttacgatgc tcaaaagact ggtcgtcaaa ccttgacgct tcgttaccaa
    27901 ggtcatgaag tcaactttga tgttttggta tctccaaaag cagcattgaa cgatgagtac
    27961 ctcaaacaaa aattagcaga agttgaagct gctaagaaca aggtggtcta taactttgct
    28021 tcaccagaag taaaagaagc cttcttgaaa gcaattgaag cggccgaaca agtgttgaaa
    28081 gaccatgaaa ctagcaccca agatcaagtc aatgaccgac ttaataaatt gacagaagct
    28141 cataaagctc tgaatggtca agagaaattt acggaagaaa agacagagct tgatcgctta
    28201 acaggtgagg ctcaagaact cttggctgcc aaaccaaacc atccttcagg ttctgcccta
    28261 gctccgcttc ttgagaaaaa caaggccttg gttgaaaaag tagatttgag tccagaagag
    28321 cttgcaacag cgaaacagag tctaaaagat ctggttgctt tattgaaaga agacaagtca
    28381 gcagtctttt ctgatagtaa aacaggtgtt gaagtacact tctcaaataa agagaagact
    28441 gtcatcaagg gtttgaaagt agagcgtgtt caagcaagtg ctgaagagaa gaaatacttt
    28501 gctggagaag atgctcatgt ctttgaaata gaaggtttgg atgaaaaagg tcaagatgtt
    28561 gatctctctt atgcttctat tgtgaaaatc ccaattgaaa aagataagaa agttaagaaa
    28621 gtatttttct tacctgaagg caaagaggca gtagaattgg cttttgaaca aacggatagt
    28681 catgttatct ttacagcacc tcactttact cattatgcct ttgtttatga atctgctgaa
    28741 aaaccacaac ctgctaaacc agcaccacaa aacacagtcc ttccaaaacc tacttatcaa
    28801 ccggcttctg atcaacaaaa ggctcctaaa ttggaagttc aagaggaaaa ggttgccttt
    28861 catcgtcaag agcatgaaaa tgctgagatg ctagttgggg aacaacgagt catcatacag
    28921 ggacgagatg gactgttaag acatgtcttt gaagttgatg aaaacggtca gcgtcgtctt
    28981 cgttcaacag aagtcatcca agaagcgatt ccagaaattg ttgaaattgg aacaaaagta
    29041 aaaacagtac cagcagtagt agctacacag gaaaaaccag ctcaaaatac agcagttaaa
    29101 tcagaagaag caagcaaaca attgccaaat acaggaacag ctgatgctaa tgaagcccta
    29161 atagcaggct tagccagcct tggtcttgct agtttagcct tgaccttgag acggaaaaga
    29221 gaagataaag attaaatatc gaaaaatctt gtgaaatctt tccttatatt tccaaagtgt
    29281 gatataatag tttcgaataa aataaataaa ggagtttttg taacatggca aaacttactg
    29341 ttaaagacgt tgacttgaaa ggtaaaaaag tcctcgttcg tgttgacttc aacgtaccat
    29401 tgaaagatgg cgtaatcact aacgataacc gtatcacagc agctcttcca actattaagt
    29461 acatcatcga acaaggtgga cgtgcaattc ttttctctca ccttggacgt gtgaaagaag
    29521 aagctgataa agctggtaaa tcacttgctc ctgtagcagc tgacttggca gcaaaacttg
    29581 gtcaagatgt tgttttccca ggtgtcactc gtggtgctga attggaagcg gcaatcaacg
    29641 ctcttgaaga tggacaagtt ctcttggttg aaaacactcg ttacgaagat gttgacggca
    29701 agaaagaatc taaaaacgat cctgaacttg gtaaatactg ggcatcactt ggagatggta
    29761 tcttcgtaaa cgatgcattc ggtacagctc accgtgcaca cgcatctaac gttggtatct
    29821 cagcaaacgt tgaaaaagca gttgctggtt tccttcttga aaacgaaatt gcctacatcc
    29881 aagaagcagt tgaaactcca gaacgtccat tcgtggctat ccttggtggt tcaaaagttt
    29941 cagacaagat cggtgttatc gaaaacttgc ttgaaaaagc tgataaagtc cttatcggtg
    30001 gtgggatgac ttacacattc tacaaagcac aaggtatcga aatcggtaac tcacttgtag
    30061 aagaagacaa attggatgtt gcgaaagctc ttcttgaaaa agcaaatggt aaattgatct
    30121 tgccagttga ctcaaaagaa gctaacgcat ttgctggtta cactgaagtg cgtgacactg
    30181 aaggtgaagc agtttctgaa ggcttccttg gtcttgacat cggtccaaaa tctatcgcca
    30241 aatttgacga agctttgact ggtgccaaaa cagttgtatg gaacggacct atgggtgtat
    30301 ttgaaaaccc agatttccaa gctggtacaa tcggtgtgat ggacgctatc gtgaaacaac
    30361 caggagttaa atcaatcatc ggtggtggtg actcagctgc cgcagcgatt aaccttggcc
    30421 gtgcagacaa gttctcatgg attagtacgg gtggtggagc atcaatggaa cttcttgaag
    30481 gtaaggttct tccaggactt gcagccttga cagaaaaata agattttata aacaaatcaa
    30541 agaagagagg gatgagagtt cctcttttct tttgcttaaa ataaaaacgc ttcctctcaa
    30601 ctattactca taaaaatcac cgatttatga taaaatggaa atagaaagtt gagattatga
    30661 gttattttaa aaaatataaa ttcgataaat cccagttcaa acttggtatg cgaaccttta
    30721 aaacaggtat tgctgttttt ctagttctct tgatttttgg cttttttggc tggaaaggtc
    30781 ttcaaattgg tgctttgaca gccgttttta gcctgaggga gagttttgat gagagtgttc
    30841 attttgggac ttcgcgtatt ctaggaaata gtatcggtgg actctatgcc ttggtcttct
    30901 tcttattaaa tacctttttc catgaagcct tttgggtgac cttggtagtt gttccaatct
    30961 gcaccatgtt aaccattatg acaaatgtag ccatgaataa caaagcaggg gttattggtg
    31021 gggtagcagc tatgttaatc attaccctat caattccaag tggtgagaca atttcgtacg
    31081 tgtttgtgcg tgtatcagaa acgtttatgg gagtttttgt cgcaattatc gtaaattacg
    31141 atattgatcg tattcgtctc tttttagaga aaaaagaaaa ataatgttac attttataac
    31201 attatcaatt gacgtttgtc tttttttaga ctataataga cagaaagaag gaaattgtaa
    31261 atgaaggaaa aagaatttcg ccgaaatatg gctgtttttc ctatcggcag tgttatgaag
    31321 ttgaccgatc tatcggcgcg tcagattcgt tattatgaag atcaagagtt gatcaagccc
    31381 gatcgaaacg aaggaaatcg tcgcatgtat tccttgaatg acatggatcg tctgcttgaa
    31441 atcaaagatt atatctctga aggttataat atcgctgcca ttaagaaaaa atatgctgaa
    31501 cgtgaagcga aatccaagaa agcggttagt cagactgaag tacgtcgtgc acttcacaat
    31561 gaactcctcc aacaggggcg ttttgcttca gtacagtcac cttttggtcg cggttaggca
    31621 accgcaagta gtcatagatt aaggagaaaa cttatgccaa tcacagctgc agatattcgt
    31681 cgtgaagtca aggaaaaaaa tgttaccttt attcgtctta tgttctcaga tattttggga
    31741 accatgaaaa acgtcgaaat tcctgctaca gatgaacagt tagataaggt cttgtcgaac
    31801 aaggttatgt ttgatggatc ttctattgaa ggttttgtac gtatcaatga gtcggatatg
    31861 tacttgtacc cggacttgga tacatggaca gtcttccctt ggggagatga aaatggaagt
    31921 gttgcaggtc tgatctgtga tgtctataca acagaaggtg aaccatttgc gggtgaccct
    31981 cgtggtaatt tgaaacgagc tcttcgtcac atggaagaag ttggattcaa atccttcaac
    32041 cttggtccag agccagaatt cttcctattt aagttggatg aaaatgggga cccaacactt
    32101 gaagtgaatg acaagggtgg ctactttgat ttggcaccta ctgaccttgc ggacaacaca
    32161 cgtcgtgaga ttgtgaatgt cttgaccaaa atgggatttg aagtagaagc gagtcaccac
    32221 gaggttgcgg ttggacagca tgagattgac tttaagtacg atgaagttct ccgtgcttgt
    32281 gataagattc aaatctttaa gcttgttgtt aaaaccattg ctcgcaaaca cggactttac
    32341 gcaacattta tggcgaagcc aaaatttggt attgctggat caggtatgca ctgtaatatg
    32401 tccttgtttg atgcagaagg aaataacgcc ttctttgatc caaatgatcc aaaaggaatg
    32461 cagttgtcag aaacagctta ccatttccta ggcggtttga tcaagcatgc ttacaactat
    32521 actgccatca tgaacccaac agttaactca tacaaacgtt tggttccagg ttatgaagcg
    32581 cctgtttaca ttgcttgggc tggtcgtaac cgttcgccac ttgtgcgcgt acctgcttca
    32641 cgtggtatgg gaactcgtct tgagttgcgt tcagtggatc caatggcgaa cccttacgtt
    32701 gctatggctg ttcttttgga agttggtttg tatggtattg aaaataaaat cgaagcacca
    32761 gctcctatcg aagaaaatat ctacatcatg acagcagaag agcgcaagga agctggtatt
    32821 acagaccttc catcaactct tcacaacgct ttgaaagctt tgacagaaga tgaagtggtt
    32881 aaagctgctc tcggagatca catctatact agcttccttg aagccaaacg aatcgaatgg
    32941 gcaagttatg caaccttcgt ttcacaatgg gaaattgata attatttaga cctttactaa
    33001 tactaatata gaagaaagat tgcctgtggg taaacagtca cacagcagtc taagccatca
    33061 aatacctata acccttaaaa tagcgatttc ggtcgctatt tatttttcga aaaatcctct
    33121 tgactctttt gcgaattaga tttatcatca gacgtgtaga agaaaattaa ggagtcacta
    33181 tggaaaaatt caatttcaaa aacaatatcg ggcaagaaaa caagttactt caaatcgaaa
    33241 tctacaagtt tactaacttt tgcaagcttc aaaattacac atctgtaaat attttttcga
    33301 aagatatatt tgaagccata gtaaattgag actagaatag tacactacat ctgctaaaaa
    33361 tttctagaaa ctaatttaac tttcctaatc gatttgttta catcttattt caatctacta
    33421 tattatctca ataaatgatg atggcgactg ttttgtttgt gcttgtcgat ttttgaatcc
    33481 tgatcgagcg gaagaatatt tctgccgagg ttgtcaaggg gaaaatcctg aagatattga
    33541 attttatgat gaacaattac aggcagaaaa ggtggaggct ttgaatattc gattggaagt
    33601 aaaaaattga attatctaca atctcatcaa caataaacct atttcaaccc cattatctat
    33661 aggcgtattt ttacgccttt ttatatactt gaagcacata
//
LOCUS       DAVHEX010000018        33435 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVHEX010000018 DAVHEX010000000
VERSION     DAVHEX010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33435
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.018"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(120..1100)
                     /locus_tag="VVL45_000820"
     CDS             complement(120..1100)
                     /locus_tag="VVL45_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEU4187021.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDDFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAVEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
     gene            complement(1438..1587)
                     /locus_tag="VVL45_000821"
     CDS             complement(1438..1587)
                     /locus_tag="VVL45_000821"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187022.1"
                     /translation="MKIKEQTRKLTAGCSKHCFEVVDETDEVSSKHRFEVADRTDEVS
                     NIYIR"
     gene            complement(1615..3630)
                     /gene="recG"
                     /locus_tag="VVL45_000822"
     CDS             complement(1615..3630)
                     /gene="recG"
                     /locus_tag="VVL45_000822"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEU4187023.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKYLAEYKQALRRIKFEELFYFQMQ
                     LQMLKSENRVQGSGLVLNWSQEKVTAVKVSLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQVYVISPLIEESEALDLKNAIALSEELTTHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            complement(3649..4752)
                     /locus_tag="VVL45_000823"
     CDS             complement(3649..4752)
                     /locus_tag="VVL45_000823"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEU4187024.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVCVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKLYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            complement(4742..5110)
                     /locus_tag="VVL45_000824"
     CDS             complement(4742..5110)
                     /locus_tag="VVL45_000824"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEU4187025.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            complement(5149..6180)
                     /locus_tag="VVL45_000825"
     CDS             complement(5149..6180)
                     /locus_tag="VVL45_000825"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU4187026.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            complement(6182..7213)
                     /locus_tag="VVL45_000826"
     CDS             complement(6182..7213)
                     /locus_tag="VVL45_000826"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU4187027.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            complement(7294..9807)
                     /gene="secA"
                     /locus_tag="VVL45_000827"
     CDS             complement(7294..9807)
                     /gene="secA"
                     /locus_tag="VVL45_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEU4187028.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYAQRYDVITADRDL
                     APEIQSMIKRTIERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRSLKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            complement(9953..10240)
                     /locus_tag="VVL45_000828"
     CDS             complement(9953..10240)
                     /locus_tag="VVL45_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187029.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDKPLLGIRIDIFI
                     IQKILLAFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            complement(10268..10900)
                     /locus_tag="VVL45_000829"
     CDS             complement(10268..10900)
                     /locus_tag="VVL45_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4187030.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKILAGYIKLDKGKVLQDGKVYGVKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDIEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQM"
     gene            complement(10904..11545)
                     /locus_tag="VVL45_000830"
     CDS             complement(10904..11545)
                     /locus_tag="VVL45_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187031.1"
                     /translation="MFKNFNNILLNRKIVLLLRIVLMMILINHLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLVLLSLLVMLIVSRLEEKFKKG"
     gene            complement(11538..12329)
                     /locus_tag="VVL45_000831"
     CDS             complement(11538..12329)
                     /locus_tag="VVL45_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187032.1"
                     /translation="MKIPLLTFARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     VKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLIIAIITYFFGTFSPLGWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYIVPYMVLEKYEDNV"
     gene            complement(12514..12849)
                     /locus_tag="VVL45_000832"
     CDS             complement(12514..12849)
                     /locus_tag="VVL45_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187033.1"
                     /translation="MEHLFKMIILLPCFYFFSWIDKDNRESKFFPIFYYFYWIYITLY
                     ALFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLAYVFFKKMLDLRRE
                     LGKSKGGRH"
     gene            complement(12989..13324)
                     /locus_tag="VVL45_000833"
     CDS             complement(12989..13324)
                     /locus_tag="VVL45_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187034.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(13413..14723)
                     /gene="der"
                     /locus_tag="VVL45_000834"
     CDS             complement(13413..14723)
                     /gene="der"
                     /locus_tag="VVL45_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEU4187035.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(14737..15450)
                     /locus_tag="VVL45_000835"
     CDS             complement(14737..15450)
                     /locus_tag="VVL45_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HEU4187036.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            complement(15447..16343)
                     /gene="dnaI"
                     /locus_tag="VVL45_000836"
     CDS             complement(15447..16343)
                     /gene="dnaI"
                     /locus_tag="VVL45_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEU4187037.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPKEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTVWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(16344..17513)
                     /locus_tag="VVL45_000837"
     CDS             complement(16344..17513)
                     /locus_tag="VVL45_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEU4187038.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLGHPVYRRLLEKKIGDVAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFSLKEATIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEEAVLRIRDR
                     GQKAKTQKQNQTAPEKTNVPKWSNPEYKNETSEETRLELERKKQELLARLEKGGD"
     gene            complement(17514..17987)
                     /gene="nrdR"
                     /locus_tag="VVL45_000838"
     CDS             complement(17514..17987)
                     /gene="nrdR"
                     /locus_tag="VVL45_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEU4187039.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            18127..18492
                     /locus_tag="VVL45_000839"
     CDS             18127..18492
                     /locus_tag="VVL45_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU4187040.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            18497..19192
                     /locus_tag="VVL45_000840"
     CDS             18497..19192
                     /locus_tag="VVL45_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4187041.1"
                     /translation="MSLLVFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            19209..19976
                     /locus_tag="VVL45_000841"
     CDS             19209..19976
                     /locus_tag="VVL45_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187042.1"
                     /translation="MFWNLVRYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLGISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAVYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVEELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(20388..21587)
                     /locus_tag="VVL45_000842"
     CDS             complement(20388..21587)
                     /locus_tag="VVL45_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4187043.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHLQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINDYAGGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(21584..22477)
                     /locus_tag="VVL45_000843"
     CDS             complement(21584..22477)
                     /locus_tag="VVL45_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4187044.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFSILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            22594..22674
                     /locus_tag="VVL45_000844"
                     /pseudo
     CDS             22594..22674
                     /locus_tag="VVL45_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            23184..23378
                     /locus_tag="VVL45_000845"
                     /pseudo
     CDS             23184..23378
                     /locus_tag="VVL45_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155340.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            23793..24410
                     /locus_tag="VVL45_000846"
     CDS             23793..24410
                     /locus_tag="VVL45_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEU4187045.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(24730..25617)
                     /locus_tag="VVL45_000847"
     CDS             complement(24730..25617)
                     /locus_tag="VVL45_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU4187046.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEMVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(25766..27649)
                     /locus_tag="VVL45_000848"
     CDS             complement(25766..27649)
                     /locus_tag="VVL45_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU4187047.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKRAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IVVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFETKV
                     AQGNKVKAGDVLGTFDSNKIAAAGLDDTTMVIVTNTGDYASVAPVATGSVAKGDAVIE
                     VKI"
     gene            28025..29479
                     /locus_tag="VVL45_000849"
     CDS             28025..29479
                     /locus_tag="VVL45_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEU4187048.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLIHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDKNWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFQGQYYAIVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEQPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            29460..30425
                     /locus_tag="VVL45_000850"
     CDS             29460..30425
                     /locus_tag="VVL45_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU4187049.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(30460..31734)
                     /locus_tag="VVL45_000851"
     CDS             complement(30460..31734)
                     /locus_tag="VVL45_000851"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEU4187050.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRIAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VVLSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(31734..32906)
                     /locus_tag="VVL45_000852"
     CDS             complement(31734..32906)
                     /locus_tag="VVL45_000852"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEU4187051.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            complement(33037..33285)
                     /locus_tag="VVL45_000853"
     CDS             complement(33037..33285)
                     /locus_tag="VVL45_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187052.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QKFVDEMGRTYSGNLILQELITVNGAYKATYIGELSSN"
BASE COUNT         9781 a         7490 c         6319 g         9845 t
ORIGIN      
        1 cctgttctta tttcatttta ctatatttcc atgataaaac gcatagtata aagttttctt
       61 aatcccctga tactatgcgt gtttgtaatt tttaagattt tgtgcttaga gtcgactcct
      121 tattttagat atttaaaagg aatctcactt ccacagagcc agttgtagac ttggtcattg
      181 acaaatacat tcatggcttc gtgagcatac tcaggcatga tgcgataggt tttatcgcag
      241 gtcagacgat tataaatcgc aaactgggta atgggatagc aaacatcgtc gtccaagccc
      301 gtaatcatct taacctcacc ttggatacga tgggcaagat ttttgacatc gatataggca
      361 agggtcgcca tgatttcctc ctctgtttca tggaaggggt cgtgaaactt gaaataacgg
      421 aaaagttcgt cgtaagcctc gctagtatta ccaatctcaa tcacccgtct gaagtctgac
      481 aagaagggat aaatggcaac tgttttctga attcgaggat tgagcgctgc tgcaactaga
      541 gctagagccc ctccttgtga ggcaccatag ctagaaagac gcttctcatc aacctgagac
      601 agactagcaa caatttcgac caactggtaa atatccagat aaacatcctt ataaaagagg
      661 tggtcccgac cttccacagc accacggata atatgcccct tcacggtatt tcctaaagga
      721 gaacgcaagc cgtcttgtga gtaacctgac tggccccgca catccatgga aacaacaccg
      781 taaccagcta cggtgaagcc cagcatgtcg gcccagtccc agccacgtcc catataacca
      841 tgaaaatgga agattaatgg gaccttctcc tcactctttg gaagaacaat gcgtgcatag
      901 acctttcctt ccttgcttcc ttcaaatgtt aactcatagc acttgacttg aggaatgtgg
      961 aaatctcttt cctccaagtg gtaggatgga agcgtggaaa catttttcac ttccccatcc
     1021 cagaaatcat caaagtcttc cggaacctca tcccttcctc tataggtctt aatttcttct
     1081 agcaaagctg gatttttcat agcatgctcc ttttattttg taatcgttac cacaactgta
     1141 gcatatctat gtaatcaatg caagaaagaa gggtaaatag aaagtgaatt tagattcttt
     1201 acttttagga tgatgggctg aaagtagttt tagaaacgaa aaaagagcat ttcgctctct
     1261 cttctgttta tactagtttt ttgcttcctt cttttggaaa gtggtttggt aatttacttt
     1321 aatcgttact gtcatatgag agtcctcgtt tctttttgat gactctagta taagagccaa
     1381 acctaaaaac tagcttaata ctcttcgaaa atctcttcaa accgcgtcag cgtcgcctta
     1441 ccgtatatat atgttactga cttcgtcagt tctatctgcc acctcaaaac ggtgttttga
     1501 gctgacttcg tcagtttcat ccacaacctc aaaacagtgt tttgagcaac ctgcggttag
     1561 cttcctagtt tgctctttga ttttcattga gtataagttt tccttagaga aagcttaatc
     1621 cagatgttct ttcttttcca gatgaagggc aatcatgcgc cactctggat cttcttgcca
     1681 agcttctata gaactaatgt agctagcaac ctttcttgct tcttctaaaa tcggaaaatc
     1741 ttcgataata tcagccactt ggaactctgg aagtcctgac tgtctggttc caaaaatctc
     1801 accagaacca cgcattttca aatcttcctc cgcaaggaca aatccattgg tcgtttctgt
     1861 catgatacgc atgcggtctt tcccagaatc cgtcttggga ttagcaacga gaacagcgta
     1921 ggactgcttg tccccccgac cgacacgacc tctaagctgg tgaagttgac tgagaccgaa
     1981 gcgatcggca tccatgataa tcatgacagt cgcattggga acgttgaccc caacctcaat
     2041 aaccgtcgtc gaaaccagaa tatccgtctt tctctccttg aaatcctgca tgatctggtc
     2101 tttttcgtca ctcttcatcc taccatgtag aagagccacc tctgccttgc ctgcaaaatg
     2161 agtcgtcaac tcctctgata aggcaatggc atttttcaaa tctagagctt ctgattcttc
     2221 aatcaaagga gagatgacat agacttggga acctttttga atttccccct ctaaccaagt
     2281 caagacctga ggtagttgct catgtttgat ccagcgcgtc acaataggct tccgacctgc
     2341 tggcatctgg tcgataatgg aaacatccat atctccaaag gctgtgatgg caagcgtccg
     2401 tggaatggga gtcgccgtca tcatgaggac atctggattg tcaccttttt cccgtaaaat
     2461 acgcctttgc cctacaccaa aacggtgctg ctcatcgata ataatcaaac caagacgagc
     2521 atactccacc ccatcttgta tcagagcgtg agttcctata atcaaatcag cctcaccctt
     2581 ggcaatggtc tccaagactt ctctcttttc tgcagctttc aaggaacctg tcaagagagc
     2641 cagtttcaaa ttgggaaaaa ggttctgtaa actctcaaag tgttgctctg cgaggatttc
     2701 tgttggtacc attagggcag cctgataacc tgctgtcact gccgcaaaca tggccaagcc
     2761 agcgactacc gtttttccac tccccacatc cccttgtagg agacgattca tgtggtggtc
     2821 ggacttcata tcagttaaaa tttcctgcaa actcttttcc tgagcttggg tcagggcaaa
     2881 aggaagactt actttaactg ctgtcacttt ttcctgagac caattcagaa ccagaccact
     2941 tccctgaact ctattttcag acttgagcat ctgcagctgc atttggaaat aaaagagttc
     3001 ctcaaacttt atacggcgaa gagcctgctt gtattctgcc aaatactttg gaaaatgcat
     3061 agcacggact gcctgacaac gggacatgag tttgtatttg tctagtaaag actggggcag
     3121 attttcttct atcaagaggt ccagtccctg atcaaaagcc gtcttgatga ccttgaccag
     3181 actggcctga ctgattccct gagccagacg atagacaggc tggaggtcat cttctacctg
     3241 agccagaacc ttcatcccag tcagactagc cttagcgcgg tcccattttc caaagacagc
     3301 aagggttgct cccaactcta ttttatcagc cagatagggc tggttaaaga aattcaccgc
     3361 aaaaacgacc tctccctgct tgagactaaa acgcaggcga ttgcgcttga aaccataata
     3421 ctggacacta gcaggagtca ctacctgacc agaaagaact gccttctcac cgtcttccag
     3481 ctccagcacc tgcttggttt tgaagtcttc ataacggaaa ggaaagtaga gcaagagatc
     3541 ttgcaagttt tcaattccta gtttggcgta tttttctgct gactttggtc ccacaccagg
     3601 caagacatgc aagggttgat gtagattcat gctccactcc tttcttttct aataatattc
     3661 tctcggaata cggtcgctga ggaggcaaac cacctcatag ttaatggtta cgcggtaggt
     3721 cgctacctga gttgcagtga tttccttatc cccattggag ccaatcaagg ttaccttggt
     3781 tcctagcgga taaagcttag gcaatcgaat agtgatttgg tccatcgaaa ccctgccgac
     3841 aattgggcaa gcttggccat ctaccaagac agagaaattt tgcatgtctc ttgtccatcc
     3901 atctgcatac ccgattggca cggtcgcgat gacttgctcg ctatccgctt gataagttgc
     3961 tccatagccc atgcaagctc cagctggaac tgtcttgaca tgaaccagag cagactccaa
     4021 ggtcaaggcc ggtatcaaat cataaggcaa atccaagacc gctccacttg gattgaggcc
     4081 atacatggca tctcccatac gaaccgcatt gaaaatagtc tctacatgcc aaagagtcgt
     4141 tgcagaattg ctagcatgaa ccagctctgg aacttccttc atactagcta aaatagtttt
     4201 aaaccgttct aactgggcat taaaatagtc atctgattcc tcatcagcag tagcaaagtg
     4261 ggtaaagatt ccttcaacac aaacaccgtg ttgttggagc aaatcttgag cctgctcaac
     4321 ctcacttgcc tctctaaaac caatccgtcc catccctgaa tcaatcttga ggtggactgt
     4381 caatccagtt aggtccactt ccttatctaa gagtgcttga atccactcca gtccagccac
     4441 tgtcaaggtg aagtcatatt ctttagctag agcaacagct tcgatttcag aaactcctaa
     4501 aatgaggatt ggcttgctga gtccagcttg tctgagttca atggcttcat cgatattgga
     4561 aacgcaaaag ccatcaacat catcttgaat tgccttggca acggcaacag ctccatgacc
     4621 ataagcattg gccttaacca cagccaactt gagcgttcct tgagggatat gagcccccat
     4681 ttgctgaata ttttgtcgaa tagctcccag atgaatcaga gccttggttg gtctatgtgg
     4741 actagctttc atgattttcc tccaaaatga cactggctgt cacaaactga tcggtgtggc
     4801 tgatagacag ccaaatcttt cctgaaaatg gtgcctgact aaaataaggc gccccacgtt
     4861 cattgttcaa gacttccaaa tcctgaaaac cgagcttgct aatgcccgtt cccatagcct
     4921 tggaaaaggc ctccttagcc gaccagcgac cagctaaata ttctatttgc ctgcgtcctt
     4981 tgagactggt gaagcgctcc atttcctgag cggtcagtac acgcttagca aatccttcat
     5041 gtcgtgtaac tgcgctttct atcgaagcca attcttcgat gtcaattccg tgtccaacta
     5101 tcattctcat caaaaggagt tgagattccc caactccatc cttttcactt attttgtcaa
     5161 ggtaacataa atctcttcta ccaaggcctc tgtattttcc caacctaggc aagggtcagt
     5221 aatagagcaa ccaaagatct ctggttggtt ttgacgacca tctgctaggt aagattcaat
     5281 cataaatcct cgaaccgtct ttttaatttt ctcattccaa tcacgattct gcaaggtctg
     5341 gcgaacaatt cgaatctgct ccatatattg cttgcctgag ttatcatggt tggtgtcaat
     5401 gaggataaaa ggattttcaa gtcccatggt ttcatagcgt tcaatggctt ggagtagatt
     5461 ttcatagtag taattcggca tataattgcc atactcgttg actgctccac ggaggataac
     5521 atgggccaaa ggattacctg atgtctcaac ttcctgccca tgataaagga aggtttgctt
     5581 gttttgagca gcatagatgg cgttaaacat aacacccaaa tttcctgagg ttggattttt
     5641 catccctact ggtgcatcaa tcccagaagc cacaaagcgg tgctcttggt cttccacaga
     5701 acgagctcca acggcatggt agctgaccaa gtcatccacc aagatcagat ttgacggata
     5761 aagcatctca tctgccgttg tcaaaccagt ctctgtaatc acgcggtagt gcaactggcg
     5821 cacagcctgc aagccattaa tcaggcttgg agccttagaa gtatctggct ggtgaactaa
     5881 tcctttatag ccgtctccat tggtacgagg cttagcagta tacacgcgca tgaccatgaa
     5941 aatcttatcc gctaccttct tttgcaaggc agataaacgg cgagcatatt ccaagaccgc
     6001 ctcttcatta tcagaagagc aaggaccaat caccaagaga atacggtcat cttcccctga
     6061 aataatatct gccaattccc tgtcacggct ttccttgagt ctcaaggctt ccgcagacaa
     6121 ttgggtttca gccttgatga cttccatatc gatttcttga cctttttcaa taaatgccat
     6181 cttattctcc taacgttttg taaatttctc tgacaagagc ttctgtgtta tcccaaccca
     6241 ggcaagggtc tgtgatagac ttaccaaata cttctggctc attttgtcga ccatcttcca
     6301 gataagactc aatcataaaa ccacgaacga actgcttaat tttttcattc caagcacggt
     6361 taatcaaggt ctggcggaca attcggatct gttcaatata ctgcttacca gaattgtcat
     6421 gattggtatc aatgatgata aaaggatttt ccaagcccat tttctcatac tgggcaatgg
     6481 tatcaattaa attgtcataa tagtagttgg gaatattttt tccatactca ttaagagcac
     6541 cacgaagaat agcgtgtgaa agcgggttcc cagttgtttc tacttctttt cctaagaaaa
     6601 ggaaactttg tttgttttga gcagcataaa tcccattaaa catgacattg agatttccag
     6661 aggttggatt tttaaaacca gtagaaaatc ctgccccact tgccacaaag cggtgttgct
     6721 ggtcttcaac tgaacgggca ccaactgcca tgtaagaaat caaatcatct acaagcggaa
     6781 ggttttcagg ataaagcatt tcatcagcag ttgtcatccc tgtttctgtg atgacacgat
     6841 agtgaagatg gcgaacggct ttgattccat tgataagact aggcgcttct gtcgcgttag
     6901 gctggtgaat caagccctta tagccatctc cgttggtacg gggtttggca gtataaacac
     6961 gcataaccat aaagatacga tctgccactt cttcttgtag gactgccaaa cgcttagcgt
     7021 attcaaggac agcttcttcg ttgtcagatg agcatggccc gattaccaag agaattcgct
     7081 ggtcttctcc acgtataatg gcttctagct cttgatctcg ctgtgatttc ctctccaaag
     7141 cctgaccttc taattttgac aaggcacgaa cttcttctat atttatttta gagctttttg
     7201 ctgtaaatac cataactcat ctccttataa agcaaacgga tacccagtaa aaatttactt
     7261 ttcacaagat atccgcctct aaactatctc attttattgt cttttaccgt gacagttttt
     7321 aaatttctta ccagaaccac atgggcaaag ttcattgcgt ccaatctggc tcaaatccaa
     7381 atcttctggc atacttgctt ggtgagcagc gatattgcga gtcgctgttg tactgatatg
     7441 acgttctgcc tgtggtcttt cttgttcatg aatttgtgct ttcatcatca agcgtgtcac
     7501 atcaaactca atcgaaccaa tcatatcatt aaacatacgg aaaccttctg cctgatactc
     7561 aacaacaggg ttgttctgag catagccacg aagtccaacc gcgttacgca attgatcaag
     7621 ggcatcgata tgatctgtcc acttgttatc caccactcgt agaatcaaaa ctttttggaa
     7681 ttctttaact gcttcttcat cgcgtagttt tgaaacctga ctatcgtaaa ccttcaagga
     7741 acgttggaaa agctcttcct tgatggcctt atcagacaag cctgacaagt cttccatcgt
     7801 aatagaatct tcaggaagca agttgtactt agcaaagttc aaaattgcct ctagtttttc
     7861 atcttgtttg gcacgcgcat gaccatcaac gacacgttca atcgtgcgtt tgatcataga
     7921 ctgaatttca ggtgccaagt cacgatctgc agtgatgaca tcgtaacgtt gagcatagat
     7981 aatctcacgt tgttcacgca tgacatcatc gtattgaagg acttgtttac gggtatcgta
     8041 gttatttcct tcgacacgtt tctgagctgc ttcaacctga cgcgtcaaca tgcgagactc
     8101 aatggcctct tcagacatgt tcaagcgttc aaagattccc ttcaagcgtt cagaaccaaa
     8161 acgtttcatc aaatcatctt caagagatag gtagaattgt gactcacctg gatctccttg
     8221 acgacctgaa cgtccacgaa gctggttatc gatacgacga ctttcatgac gttctgtacc
     8281 aataacacaa agtcctccaa gttcacgaac accttcacca agcttgatgt cggtaccacg
     8341 acccgccatg ttggttgcga tggtaacggc accacgttga ccagcattca tgatgatttg
     8401 ggcttctcta tagtggtttt tggcattcaa gacttcgtga ggaacaccag ctgcaaccaa
     8461 tttcttagaa atgtagtcac tagtttcaac cgctactgta ccaaccaaga caggttgacc
     8521 cttttggtaa cgagccttaa cgtcttcgac aaccgcttta aacttagatt cgatacttgc
     8581 ataaagaagg tctgagtggt caatacgttg aacaggacgg tttgttggga ttggaataac
     8641 acgaatgttg tagatttcac ggaattcttc ttcctcagtc ttacctgtac ccgtcatacc
     8701 agacaatttc ttgtacatac ggaagaggtt ttggtacgtg attgaggcag atgtcttggt
     8761 ttcatcctgg attggcacac cttctttggc ttcaatagct tggtgcaatc catcagaata
     8821 acgacgacct tccatggtac gacctgtaaa ttggtcgaca atcaagattt cttgctcttc
     8881 gctcaccaca tagtcaatat cgagaagcat gatgtagttg gcacgaaggg cgttatcgat
     8941 aaagtgagtc aaagccacgt tttcgatgtc atagaggttt tcaagtttga agtagctttc
     9001 agccctgtca atccctgaat cagacaaacc aatagtctta gactgcacat cgatgatgta
     9061 gtcatctttg ttcaaagatt ttacatagtg gtctgccatg tgatacaact gactggtttc
     9121 aaccgcattg gcacctgata caatcaaagg tgtacgagcc tcgtcaatca agatagagtc
     9181 aacctcatcg accaaggcat agttaagcgg acgttgtacc atgttttcgg cgcgaacgac
     9241 catgttgtca cgaaggtagt caaatccgat ttctgagtta gttgagtaag taatatcaca
     9301 ctcataggct tcttttttct ccattggaga tttggtagcc aagttaatcc ctactgacaa
     9361 accaagccaa gagtacaatt cacccatctc agtcgcgtca cgttctgaca ggtattcatt
     9421 aaccgtaact acgtgaaccc ctttacctga aagggcattg aggtataccg gcatggtcgc
     9481 agtcaaggtt ttcccttccc ctgtacgcat ctctggcacg tcaccatggt gaagaacaat
     9541 cccccccatg acctgaacct tataagggaa gagacctagg acacgtttgg caccttcacg
     9601 gacaaccgca aatgcttcgt aaagcaatga atccagtgat tctccatttt gataacgttc
     9661 cttaaattca actgtttttg cttttagttg gtcgtcagtc aaagcagcca tttggtcttc
     9721 gtatttgaaa accttgtcag ccatcttttc cagacgacgg atttctcctt tatcattttc
     9781 gataattgtt tttaaaatat tagccatgtt tttccttact ttaaattccg aatattttag
     9841 aatgttcctt taattttagc acaattactg attattttca aggaagaatc cccattttga
     9901 aaaggaaaaa gaggttagtt ttcaagctaa cctctccgac ttttatgact gtttaattgc
     9961 caaaaatcgg caaaatggca aatgtgataa ataagttaat ccaaagagaa agacagcaaa
    10021 tcagtccaaa aacaaagaga ctgactttct tggacagcaa ggccatgaga atggacagaa
    10081 ttccaaaagc tagcaagatt ttctgtatga tgaagatgtc aatccttatc cccagaagtg
    10141 gtttatccca agggataaag aagaaagaca gccagatcag cagaactaaa ccaatataaa
    10201 ccttggaata tcgtgtaata aatgatttta gatgtgccat aagccatctc ctaggattgt
    10261 atatccttta catttgtata tgtccatttt cgacaactaa tacatctgta caaaggtctg
    10321 aaatatcttc cgatatgtgc gacgtcagga taaccagacc attttctttt ttcaggtaag
    10381 ataaaatgac ctgtttggtt aacctcacac tcttctcatc caaagcattc ataggttcat
    10441 cgagaaagag tatagaagga gaggaaataa aggcttgaat caaggccatt ttttgctttg
    10501 ttcctaagga taaatgacgg tattcaatgt cttcaaattc ctgtaaatca tagtattgaa
    10561 tccaataggc aattcttttt tccgtaactt tagatgaaaa ataccttaac agttccaaat
    10621 tttctctcag ggataaatga gataaaaact cgactttttc aattaaaatt cctgcatcct
    10681 gaatataatg attttttacc ccgtaaactt taccatcttg aagaactttt cctttgtcaa
    10741 gcttaatata accagcaagt atctttaaaa gaaccgtctt gccagatcca ttatcacctt
    10801 taagtccata aatttttcct gattgaaaca ccaaattcaa ctgatttaaa ataggtcttt
    10861 tcccataaat tttctgtcca tttataattt caagtctcat cgtttatcct ttcttaaatt
    10921 tctcttccaa gcgactaaca atcaacataa ctaataaact taagagtact aaatacactg
    10981 ttacaatatg atccatcatc cattcttgtc ccacaaatgc taaaaaatag agaaaaaagg
    11041 aaaaaccagc gcccgcacta atcagtgcta tcagataaga cagtagtaag tacaacatca
    11101 ttaactgagc aagtaaaaag aaaagtgcga ctgttttgaa atgaattcct agaggaagta
    11161 aaccaagaaa ccagacaaac aaatcttgag taaaaaattt agaaatgaca accaatcgtt
    11221 tccttgtata atcgaaaaag gagcttgatt gatagcgaat caagtgatgg taatggtctg
    11281 ccaaatcact ttcaagtaaa atgacagaga gccatcctac catgaaaagc gaaaggttta
    11341 acaatagttt ggggatttct aaattcgctt cagaatagtc attataggaa aaaattgaaa
    11401 tcaattctct cttgaaaaag ataacagcat cctgcttttg aaccgctgtt gacaatagat
    11461 ggtttatcaa aatcatcatc agaactatac gaagtagtaa aacaatcttt ctatttagca
    11521 aaatattgtt aaaattctta aacattatct tcatattttt caagcaccat gtaagggaca
    11581 atataaagga tataaggagt aaagagtttc atcaaactta catccccata gctagcttgc
    11641 atcaaactcg tcataggaac catatagtaa ggcaaggcac tataaagcag catagaacca
    11701 agccaaagaa acatcaaata ggtgattgcc gaacgagtca cattccccac ataatcaact
    11761 atttgtaaaa aatagattgc attgatgaaa ataccgatta gtaggacaca agtaaagaac
    11821 aaatagcttt ttatctctcc atctaggagt ctttgtaaac cacttccatc agaaaataga
    11881 gaattccatc caagaggaga aaaagtccca aagaaatagg ttataattgc aattatcagc
    11941 acagtcacta aatatatcaa acaagggata cttgcaactt gcaaagtcaa ctttcttttt
    12001 aacccttgat aactcacttc tcttccaata ctcaatcgta aaactttatt tttcagctca
    12061 atatattgaa aacctagcaa aacaatgatg attggaataa taaatgattg ataaaaacct
    12121 agattaaact ggagaatacg aaaatctaat gctgatttta ataagtcatt tttaattgct
    12181 tgtccatcga atttaactag atcgggcgca tgaatatcaa aaaagaaata agtcatcaaa
    12241 acatcacgat aaaccaaagc aagaaaaagc aaagtaagca agacataaac aaatttatgc
    12301 cttgcaaaag ttaagagagg aatcttcata agccacctcc tacaaataga aagtaatata
    12361 atactcaatg aaaatcaaag agcaaactag gaaactagcc gcaagttgct caaagcactg
    12421 ctttgaggtt gtagataaga ctgacgaagt cagttacata tataatccaa ggcgacgttg
    12481 acgtggtttg aagagatttt cgaagagtat aaatcaatgc cttccgcctt tagacttccc
    12541 tagttcccgt ctcaagtcaa gcattttttt gaaaaagaca taggctaacc agtcactagc
    12601 aaaagcgata agtagtaata atagccaaat accccataac ttgatatctg tcaaatttct
    12661 caagacgata ttgaaaaaca gaactgaaaa aactgtccaa gcaaggctaa aaagagcata
    12721 gagggtgatg taaatccagt aaaaatagta aaaaattggg aaaaatttac tttctctatt
    12781 atccttgtca atccagctga aaaagtaaaa acagggtaat aagattatca ttttaaacaa
    12841 gtgttccatc acaaacctcc tcttttaaat tattttcttt agtttcgttc tattaaatgt
    12901 tattaccaat ataaaccttg gaatagcgtg taataaatga ttttagaggt accataagcc
    12961 acctcctaca aatagaaacc gatataaatc aatgccttcc acccttagac ttccctagtt
    13021 cctgtctcaa gcgaaacatt tctttgaaac aggaataagt taaccaattc ataccaatag
    13081 ctagcagaat aaaaagaaac caaatgcccc ataacttgat atctgtcaca tttctcaaga
    13141 cggtattgaa aaacagaact gaaacaactg tccaagcaag gctaaaaaga gaatagaagg
    13201 ggatgtaaaa ccagtaaaaa taataaaaaa ttggaaaaaa cttactattt ctgttggcct
    13261 tttcaatcca gttatcaaaa taaaagtacg gtgctaaaag taagaattta aacaaatgtt
    13321 ccatcaccga catcccccct tcttttgata gcgttttcta ttattttatt atatcaaaaa
    13381 aatccggaac tgtcattcca gattctactt ttttatttgc gttttcttgc gatgagatga
    13441 atcggtgttc cctcaaaaac aaaggccttg cggatttgat tttccaagaa acgcaggtaa
    13501 gaaaagtgca tgagttcttc ttcattgaca aagatgacaa aggttggtgg tttggttgcc
    13561 acttgggtcg catagaaaat cttgagacgt tttcctttgt ctgtcggtgt tgggttgatg
    13621 gcaatggcat ccatgatgac atcgttcaag acagctgatg gaatacgtgt attttgactt
    13681 tcgctgattt gcttaatcat ctcaggaagt ttgtggagac gttgcttggt taaagctgat
    13741 acaaagataa tcggtgcgta aggcaggtat tggaactgct cacggatatc ttcttcccag
    13801 tttttcatag tgtggttatc tttttcaagc gtatcccact tgttgaccac gataatcatc
    13861 cctttaccag cttcatgggc aaatcctgcg atacgcttgt cgtactcacg aatgccttct
    13921 tccgcattga tgaccatcaa gaccacatct gaacggtcaa tagcacgcat ggcacgcata
    13981 acagagtatt tctcagtatt ttcataaacc ttaccagact tacgcatacc agccgtatca
    14041 atcatggtaa actcttgacc atctgtatct gtaaagtggg tatcaatggc atcacgagtt
    14101 gttccagcaa caggactagc aataacacgg tcttctccca agatagcatt gatcaagctt
    14161 gattttccaa cgttaggacg accaatcaag ctaaacttaa tgacatctgg attttcttcc
    14221 tcatattcat ttggaagatt ttctacgatc gcatctagca catcccctgt accgattcca
    14281 tggacagatg agataggcaa tggttcaccc aaaccgagag catagaaatc atatatatca
    14341 tttctcatct cagggttgtc caccttgttg actgcgagga taactggttt gtgggtctta
    14401 taaagcttac gagctacgta ttcgtctgca tcagtaattc cttccttacc agacacgaca
    14461 aaaacgataa catctgcttc ttccatggca atttctgcct ggtgcttgat ttgttccatg
    14521 aaaggagcat cgacatcatc aattcctcct gtatcaatca tgctaaaaga acgattgagc
    14581 cactcacccg ttgcataaat acggtcacgt gtcactcctt cgacatcttc tacaatggag
    14641 attcgctcac cagcgatccg attaaatagg gttgatttcc caacattggg acgtcctaca
    14701 atggcaatag ttggtagggc cataatttct cactttctac aataatttct tctgttcaag
    14761 attttttcta gttgagcttg gttcagcttg accaaactgt tctgctaggc gctgactcca
    14821 gcttgtggtc gcacgcgccc cagcatagtc agcctgaaca cggtcataag cttggattgc
    14881 ctcagttgac tgttcttggt attcttcctc aaagacaaca ttctctagtg gcagtctcgg
    14941 tttcatatca tgatgttgat ttggcacacc cagtgccatc ccaaagacag aataggtgta
    15001 gtcaggtagg ttaaagagct ctgccacttc ttcagacttg tatcgaacca aaccgataat
    15061 cacaccacca tagcccaagc tttcagctgc caacaaggcg ttttgtccag caagagctgc
    15121 atcgaccgaa ctaatcaaga gaccttccac accttggggt tggaaggtgt cggtatgaag
    15181 tcgggctccc ttttctgctc ggttcaaatc tccgacaaag agaaggaaaa cagcagactg
    15241 gcgaatggct tcttgaggta ccaattcata caaggcatct ttcttctctt gacttcgtac
    15301 cacaatcaca gagtaggatt ggaaattctt ccaagatgat gccatctggg ctgctgtcaa
    15361 aatctcattt aagtctactt ggggaatttc ttgctcttta aacctgcgca ctgaagtatg
    15421 agccttcatc aatttaatgg tttctgtcat cgacggttta ctccttctaa acgagtctcc
    15481 tcagccaaat aacggatgcg ttccatgacc cgtctggctt cccaggtttc gtcatttcca
    15541 tgtttcactt tcgcaaaatg cttctccaaa tcttcaaagt tgaagttgga tgtgaaaaag
    15601 gtcggtaaat tttcctgcat ccgatattgg agaatgacct gcaggatttc gtcacgcacc
    15661 caaacggttg attgctcggc gccaatatca tctaaaatca ggacctcaga cagcttaatc
    15721 tcatccacca aggtcttaac attgccatca ctgatagcat ttttgacatc aatgacaaag
    15781 ctaggatagt ggaggagagt tgatgaaaca ccacgttttt ctgataaatc atgagctaag
    15841 gccgccacca tgaaactttt acccacacca aagtctccat ataagtaaag accttttcga
    15901 atagctggat attgctccac gaaggctagt agcttttcaa aaactggtaa gcgccccaaa
    15961 tcatccaagt caacttgagc caaactagct ttcttgagac tggctggtag attgattaac
    16021 ttgagacggt tcttaatagc cgcttctttt tcagccgcga ttagctcagg agtttcttca
    16081 tatgaaacat ctgcataacc atgattctta accaaaatcg gcttgtagcc tttggcaata
    16141 taatccgtat ccccacggag aaacttgtca cgctcggtga tgtactgatt aaacttggag
    16201 atactgcgat ttaattcctt tggagttaag gattcttgct ggataaaggc cgcaacatca
    16261 gggtccttca tgattttctg gaccaaatct tgataataaa aacggctggg ttgacgtttg
    16321 agtacgtctc cgacactttc catctaatct cctccttttt ctaatcgagc taatagttct
    16381 tgcttcttac gttctagttc cagacgagtt tcctcgctgg tttcattctt atattcagga
    16441 ttactccatt taggaacatt ggttttttct ggggcagtct gattctgttt ttgtgttttt
    16501 gctttctgcc ctcgatcacg aattcgtaaa acggcctctt ctgccgaatg aatcttttga
    16561 taggcatagt cattggctac cttcatggca tatttctcat tgatatttgc cgaatccacc
    16621 ttattaaagg tcaataagag aataatattg atgacttcgt ccagtaagcc caagccagcc
    16681 atctgttgca agagttctct ttctgtttgg gtaatggttc ccttgcgtgt ttgcttgatt
    16741 tctgctaaga actgcagggc agttttactt ttagcttctt tgataatggt cgcttcctta
    16801 agactaaagt cagaggaaac tggtttttga gcaatttttt cacgcatgcg tttggttgaa
    16861 ataacctggg aaacagctgt tgacttggcc aattgatagg tttcaaacca agtccatttc
    16921 ttctcctcgg caatagcaaa gaggtttaag acatcggact gctcatccgc aaaacgaagt
    16981 ccatctcgag ccatcagctg gcgaaaatgt tccaagtcaa aatcattggc cactttcttc
    17041 ttgagaccaa ggtcttcttg actgcctagt tctgccaatt ctggaaagac ttgattgagt
    17101 gagacaggta tttcttcacc atcagcactt tcaactttca aatcctccac agctacatcg
    17161 ccaatctttt tctctaagag tctgcgataa acaggatgcc ccaagaagtc ttgactagat
    17221 agaggagcat ggagggctag ctgataaaca tcaccctttt gatagagggt caagagatta
    17281 aaagcagata agattttcaa tgattttatc agtctatcca tcccaaagtt gagatggttg
    17341 agaatgcttg aaaaaagata ttcctttcta ccattatccc aaaaactgat tgtataaaga
    17401 taaaggctca gtgcctcctg accgataatc gggaggtagc actgtaccag agatgaggta
    17461 tcttgcgaca cccgattatt ctttagataa gaaaaacggt caattggctt catttatctt
    17521 tcctttttct ttttagagga ctgggtgatt tgttggagca agctctctaa ctcactgaca
    17581 tccttaaaac tacgatagac actagcaaaa cgtacatagg taatctcgtc caattcagcc
    17641 aactcctcca tgacgagtga accaatgtcc tcactttgaa tttcattttc atttcgacca
    17701 cggagtttct gttcgatacg attgactacc atgttgattt catcacttga cacaggacgt
    17761 ttctgggctg agcggataat cccattaaag attttatctc tggagaattg ttcccgtgtg
    17821 ccatcttttt taacaaccac taaggttctt tcttctactc gttcgtaggt tgtaaaacgg
    17881 tgttggcatt cgtcgcactc acgtcttcta cgaatggtgt tcccttcttc tgcttggcga
    17941 ctatcgataa cacttgactt ggtagcccca cattttggac aacgcatcct ttccctcctt
    18001 atcgttttct tttcattata ccatttttta agcgattccc aaaacaattc ttctttttgc
    18061 ttgacaagtt ttttgttttg ttgtattatt taattaagac aacaaggtaa aagaaaggag
    18121 actaagatgt cctggacatt tgacaacaaa aaacccatct atttacagat tatggagaaa
    18181 atcaagcttc agattgtttc ccatacactg gagcccaatc aacaacttcc aaccgtgagg
    18241 gagctagcta gcgaggctgg tgtcaatccc aataccatcc aaagagcctt atcagacctt
    18301 gaacgagaag gatttgtcta cagcaagcga acaactggac gatttgtgac taaggataag
    18361 gagctaatcg cccagtcacg caaacaatta tcagaagaag aattggaaca cttcgtttcc
    18421 tccatgaccc attttggcta tgaaaaagaa gaactaccag gcgtagtcag tgattatatt
    18481 aaaggagttt aagcctatgt cattactagt atttgaaaat gtatccaaat catatggagc
    18541 aacaccagcc cttgaaaatg tttctcttga cattccagct ggaaaaattg tcggccttct
    18601 tgggccaaac ggctcaggaa aaacaaccct gattaaacta attaatggcc tcttacaacc
    18661 agatcaagga cgtgtcctca tcaacgacat ggacccaagc ccagcaacca aggccgttgt
    18721 agcttatttg cctgatacga cctatctcaa tgagcaaatg aaggtcaaag aagccctaac
    18781 ctacttcaag accttctata aagatttcaa tcttgaacgc gcccatcatc tacttgcaga
    18841 cctgggcatt gatgaaaata gtcgtctcaa gaaactatca aaaggaaaca aagaaaaggt
    18901 tcaactgatt ttggttatga gccgtgatgc tcgtctctat gttttggacg aacccattgg
    18961 tggggtggat ccagcagccc gtgcttatat cctcaatacc attatcaaca actactcacc
    19021 aacttctacc gttttgattt ctacccactt gatttctgat atcgagccaa tcttggatga
    19081 aattgtcttc ctaaaagacg gaaaagtcgt ccgtcaagga aatgtagatg atattcgcta
    19141 cgagtcaggt gaatccattg accaactctt ccgtcaggaa tttaaggcct aagcaaagga
    19201 gattatttat gttttggaat ttagttcgct acgaatttaa aaatgttaac aagtggtatt
    19261 tagccctcta cgcagccgtg ctagtccttt ctgccctcat cggaatacag acacaaggct
    19321 ttaaaaatct accttaccaa gaaagtcagg ctactatgct actttttcta gctacagtct
    19381 ttggtggctt gatgcttaca cttgggattt caaccatttt cttgattatt aaacgcttca
    19441 aaggtagtgt ctacgaccga caaggctatc tgactttgac cttgccagtt tctgaacacc
    19501 atatcatcac agccaaacta atcggtgcct ttatctggtc attgattagc accgctgtat
    19561 tggctctaag tgctgttatt attctggctt taacagctcc agaatggatt cctctttctt
    19621 atgtgattac atttgtagaa acacatctcc ctcagatctt tcttacaggt atatccttcc
    19681 tactaaatac tatttcagga atcctctgca tctacctggc tatttccatt ggacagcttt
    19741 tcaatgaata ccgtacagca ctcgctgttg cagtctacat tggtatccaa atcgtcattg
    19801 gatttattga acttttcttc aatcttagtt ctaatttcta tgtcaattca ctggtaggac
    19861 tcaatgacca tttctatatg ggagcaggta tagccattgt tgaagaactc atattcatag
    19921 ctatctttta tctcggaacc tactacatct tgagaaataa ggttaatttg ctttaaataa
    19981 tttttaccta gatatgtaac atactcatag aacaaaagag accaggcaaa aagtctttaa
    20041 aattagaaaa cgcatagtat caggtgttga atatgtactg ccccccaaaa gttagatttt
    20101 ttctgtctaa cttttggggg cagttcataa gaaccttggt aatatgcgtt ttttgtgagc
    20161 tgacttattt cctttcacta tatcgcaaaa tgaaataaga acggaacgat gggattttgg
    20221 aattcaaatc aatttataag aatgttttag aagtaatatt atcctattcc agattcagtt
    20281 cactatacaa ttgagttttc aagcaacctg tttacataat gtgtacataa ttaggttcgt
    20341 gattccaccc ttttcacctt taaaaacctc gctttcgcaa ggctcttcta tttataagat
    20401 aaggcacgtt taaaggtttt ccaaatccct aaatcatccg tttgaagaac gagactagca
    20461 tacatgcgtc cgataaatcc tgttgctacc accgcaaaaa tcactgtaat agcaagtgaa
    20521 atccatgctt ctgctccccc cgcatagtca ttaatcgttc gaaacggcat aaagaaggtc
    20581 gaaataaagg gaatataaga accaatcttc aagaggagat tgtcaccagc tgcacctaga
    20641 gctgtcactc caaaaaaacc acccataatc aaaatcatca aaggcgacaa ggctttccct
    20701 gagtcctcag gacgagaaac catagatcct aggaaggctg ccaagactac gtacatgaaa
    20761 agactgatca aaataaagag caaggtattc agtgagatag catctcccaa gtgatccaaa
    20821 ataccagact gagccaagaa tggcaaatct ttaaagagca aaacggcagc cagaccacct
    20881 acaacataga tcccaatatg cgttaaaatc actagaaaca gagccatcat ccgcgcatag
    20941 aaatagtgac ttgcccttat gctagaaaaa acgacttcca taattttggt gcctttttca
    21001 ctggcaactt cctgagctgt tacacccgca taggtaatca gaatcatata aagaaagaat
    21061 cctaaggcac ctgctgcaat tgtttgaata aactttttat tttccttggc ttcatcaatc
    21121 ttttctgtga attgaattgt ctgcgctaag cgtttttcct gctcttgaga caaggaagca
    21181 gttgaacgat taagctgatt ttgcagttca ttgagtgtac ctgtaacctc aaatttaatt
    21241 ccattttcaa gcgatgtttc gccatgataa actgccttta gaacactatc ttcttgatca
    21301 atggtcaaat aaccttttaa tttttcttct ttaattgctt ctttggcact tgcttcgtct
    21361 ttatagtcga agttaacacc atttacattc ttcagtcctt ctgctacaga tggcactgtt
    21421 gtcactactg ccactttatt atttttagcc atagaagaac cttggagatg cccaattcct
    21481 acagagattc ctaaaaagag gaacggcgaa atcaccataa agaagaaact ccatgactcg
    21541 acatgtcgaa gataggtttc cttgattaca acccacatat ttctcatact tccactcctg
    21601 attctagttt aaagatttca tcgatagttg gcgcttgttg gtcaaatgtt gcgatatatt
    21661 gaccttgagt caagattgag aagagttccc ttccagcgct ctcatcctcc aaaatcaatt
    21721 tccaactgcc ttgtttggtc aagctcacct gtttgacatg aggaagattt tccaattctt
    21781 ccttgcttcg ttcacttgaa acaaagagac gcgttttccc gtattgattg cggacatcct
    21841 gaactggtcc gtgcaagacc acacggccat ctcggatcat cagaatatcg tcacaaagtt
    21901 cctcaacatt ggtcatgaca tggtcagaaa agataatggt tgctccgcgc tctttttcct
    21961 gaaaaatgac ttgtttgagc aattctgtat taactgggtc caatccacta aaaggctcat
    22021 ccaagataat caggtctggt tcatgaatca gagtaataat gagctgaatc ttctgctgat
    22081 ttccttttga cagactcttg attttatctg tcagctttcc tttcacttcc aacctcttca
    22141 tccattgagg gagtttttct ttgacttctt tggcatccat gccttttaga gtcgccaagt
    22201 agcgaacttg ttcaagaact gtcaatttag gcatgagact gcgttcttca ggcagataac
    22261 caatccgagc ataggtctcc tgacgaatat cctgaccatc cagaccgatt tctccctgat
    22321 attctaggaa tttcaaaata ctatggaaaa tcgttgtttt tccagcacca ttttttccga
    22381 ctagtcccaa aatacgacct ggtcgcgctt gaaagtcaat accaaacaaa acttgcttgg
    22441 atccaaaact tttctctaga cttcttactt ctagcatctt tcacctccga aatttcttgc
    22501 actcattata ctcctttttg atagccttta caatgttttt tgtccatttt tagaagacta
    22561 ttgctgtgta aaatatggcc tggagcactt ttatactcaa tgaaaatcaa agagcaaact
    22621 aggaagctag ccgtagactg ctcaaagtac agctttgagg ttgcagataa aactgacgaa
    22681 gtcagctcaa aacactgttt tgaggttgtg gatagaactg acgaagtcag taactatatc
    22741 tacggcaagg cgaagctgac gtggtttgaa gagattttcg aagagtatta gtgataaatc
    22801 cattatacag cagcaaactt aatttatacc ttccgctcct caactgtcta tttttaatcc
    22861 tgaattgtta tttgagtaac tcctttttcc tcgtaaagtt ttcttcctct aaaacttctg
    22921 gaaaaaggct aatagtttca gacaacattt ttataagaaa caagttcatc tgtcatttca
    22981 agaaggagta atcctttatc tactaattga cggaacagaa ttcaaccgct tgtccgatat
    23041 gttttctaag gattatatag taaaatgaaa taagaacagg acaaattgat caggacagtc
    23101 aaattgattt ctaacaatgt tttagaagta gatgtatact attctagttt caatctgcta
    23161 tatctattat gcacacccct ataggatcta atgaaaatca caacaggctc attcatagat
    23221 ggttacctaa gcctaaggga actaagaaaa cgactaccaa ggaagtcgca ttcatcgaaa
    23281 agtagattaa caactatcct aaaaaatgct tgaactacaa gtcccccaga gaagacttct
    23341 ggatgactaa cttgaacttg aaatttagca ataattaatt cactatctaa ctatatttag
    23401 taattatttc agaactgatt aatattaaaa ttaactaaca attcaaagga ttcatactag
    23461 ccataaatta cgtccatcag agagagactc ttactacttt tagattttag tctttctagc
    23521 ttcagaatac atctaaactt tagggaaaat gactattcga aagcgcgaat gcctcaaaat
    23581 tatctcagat aagctattcg aaacttagaa tgcttttaaa tttatggaat tgcgattatt
    23641 cgaaacctag aatgcatata acctttagtt gacagaccta ttctaagtct cgaagggcta
    23701 tttactttct attccttatc aaaaaagact cattccccct ttctcctcca aaatatggta
    23761 tagtagaaat atactatcta tgaggagttt acatgtcaca ggataaacaa atgaaagctg
    23821 tttctcccct tctgcagcga gttatcaata tctcatcgat tgtcggtggg gttgggagtt
    23881 tgattttctg tatttgggct tatcaggctg ggattttaca atccaaggaa accctctctg
    23941 cctttatcca gcaggcaggc atctggggtc cacctctctt tatcttttta cagattttac
    24001 agactgtcgt ccctatcatt ccaggggcct tgacctcggt ggctggggtc tttatctacg
    24061 ggcacatcat cgggactatc tacaactata tcggcatcgt gattggctgt gccattatct
    24121 tttatctagt gcgcctatac ggagctgcct ttgtccagtc tgtcgtcagc aagcgcacct
    24181 acgacaagta catcggctgg ctagataagg gcaatcgttt tgaccgcttc tttattttta
    24241 tgatgatttg gcccattagc ccagctgact ttctctgtat gctggctgcc ctgaccaaga
    24301 tgagcttcaa gcgctacatg accatcatca ttctgaccaa accctttacc ctcgtggttt
    24361 atacctacgg tctgacctat attattgact ttttctggca aatgctttga cacgtaaaaa
    24421 atccgtttgg tttcccaagt ggatttttaa agcgtagatt aactatagct tgatactaaa
    24481 tatactttgg tatggaaatc atgcatattt ttcgatagtg aggcgaggac ttacctagcc
    24541 tttccgccgt gatagaaaca cctgaaatct aatggtttca ggtattcgga aactttgagc
    24601 ctagtgtctc aaagtttagg tatggaattt tgaagaaagt cgctaccgtc cgtaatcact
    24661 taaggaaagg ctcaaaaata ttgttttcaa ccacaaaatc cgtttggttt cccaagcgga
    24721 ttttgtgctt tattttgaaa cttcttttgc aagaacaaag ttcccaagtg tggcagaacc
    24781 atttcctgcg actgctggcg tcacgatata gtcacgcaca tctggtactg gtaggtaacc
    24841 attaagaaga gatgtaaatt tctcacggac acggtccagc atatgttgtt gagccatgac
    24901 ccctccacca aagacaatca cgtctgggcg gaaagtcact gtcgcattaa ccgcagcttg
    24961 agcgatatag taggcttgaa catcccaaac agggttgttg agttcaatag tttccccacg
    25021 tacacctgta cgagcttcca aacttggacc agctgcataa ccttctagac atcccttatg
    25081 gaaaggacaa acacccttaa actctttttc aatatccatt gggtgtctag caacataata
    25141 atgacccatt tcagggtgac ccacaccacc gataaactca ccacgttgga tgacgcctgc
    25201 accgatacct gtaccgattg tgtagtaaac caagttttcg atacgaccac cagcattgtt
    25261 acgggcaacc atttcaccgt aagcagagct gtttacgtct gttgtgaagt acattggcac
    25321 gtttagggcg cgacgaaggg caccaagcaa gtctacattt gcccagtttg gttttggagt
    25381 cgtcgtgata aagccataag tttttgagtt tttgtcaata tcaatcggcc caaatgaacc
    25441 aactgcaaga ccagcaaggt tatcgaattt tgagaagaac tcaatggttt tatcgattgt
    25501 ttcgattgga gttgttgttg gaaattgtgt tttttctaca acgttaaagt tttcatcacc
    25561 gacagcacag acaaactttg taccgcccgc ttccaagctt ccatataatt ttgtcatgat
    25621 aaacctcttg tttttatttt ctttattata gcatacttcg aaagtctaaa tgtctctatt
    25681 ttttagattt tcctctgtaa atcttactat ctaataaaaa cgaacaaaca tgtcatttgt
    25741 tcgttttcac attagagagg attgattaga ttttcacttc gatcacagca tcccccttag
    25801 caactgaacc tgttgcgact ggagctactg aagcgtagtc acctgtattt gtaacgataa
    25861 ccattgttgt atcatcaagt ccagctgcag cgattttgtt tgagtcaaat gttccaagaa
    25921 catcgccagc tttcacctta ttaccttgag caacttttgt ttcaaaaccg tcaccgttca
    25981 tagatacagt atcaatacca acatgaatca aaacttcagc accatttctt gttttcaaac
    26041 caaaagcgtg ccctgttgga aaggcaattg aaacttcagc atcagctggt gcatagacca
    26101 cgccttggct tggtttcaca acgatacctt gtcccatagc tccacttgag aagactgggt
    26161 cattgacatc agcaagagcg acaacatcac cgacgatagg agttacaagt gtttcatttt
    26221 gaagagctgc tggcgcaact tcttcttttt cttcagccac ttcagctcgt tttgcagctg
    26281 cagttgcgtc tacttcatct tcgtaaccaa acatgtaagt aagagcaaaa ccaagggcaa
    26341 atgatacagc taccataaga aggtattgtg gaagttgtcc gttaccaaca taaagcattg
    26401 taccagggat gatggtgata ccattaccag taccagcaag tccaaggata gaagccaatc
    26461 caccaccgat tgcaccagca atcaatgaaa ggaagaatgg tttacggaag cgcaagttca
    26521 ccccgaagat agcaggctct gtaataccta ggaaggcaga aagagcagcc gggaaagcaa
    26581 gtgttttcag ttttggattt tttgttttaa caccaaccgc aacagtagca gcaccttgag
    26641 ctgtcatagc agctgtgatg atagcgttga atgggttagc atggtcagca gcaagtaatt
    26701 gcacttcaag caagttgaag atgtggtgca cacctgacac gacgatcaat tggtgaaccc
    26761 caccaatcaa gaaaccacca agaccaaatg gcatgctaag aatcgctttt gtagcaataa
    26821 ggatgtagtt ttcaacaacg tggaaaactg gtccaatgac aaagagtcca aggatagaca
    26881 tgaccaaaag tgtcacgaat ggtgttacca agaggtcaat gacatctgga acaaccttgc
    26941 ggacagcttt ttcaaattta gctccgacaa ccccgatgat gaaggctgga agaacggaac
    27001 cttgcaaacc aacaacaggg atgaaaccaa agaagttcat cgctgttact tcaccacctt
    27061 gagcaactgc ccaagcgttt ggaagtgagc cagagacaag catcatacca agaacgatac
    27121 caacggcagg atttccacca aatacacgga aggttgacca cacaaccaaa cctggcaaga
    27181 tgatgaaggc tgtatctgtc aagatttgtg tgtaagttgc aaagtcacct ggaagtggca
    27241 tttcaagagc gttgaaaaga ccacgcacac ccatgaagag acctgtcgct acgataactg
    27301 ggatgattgg aacgaaaaca tcaccaaaag tacggatagc acgttggaac cagttccctt
    27361 gtttagcaac ttctgctttc atgtcatcct tagatgatgt tggtaatcca agtacaacaa
    27421 cttcatcgta cattttgtta actgtacctg taccaaagat aatttggtat tgccctgagt
    27481 taaagaaagc accttgaact ttttccaagt tctcaatcac ttctttattg attttctctt
    27541 catctttgac catgacacgt agacgagtcg cacagtgggc aacactattg acattttcac
    27601 gtccgcccaa ggcatcgatg actttttttg caatttcctg attgttcatt tgcaaaaatc
    27661 tccttatata acattttgtt cttgtttgaa agcgatttta ttcgccctta cgactattat
    27721 tttatcatgt tttaaaaata tgtcaagcgt tttgcagaaa aaaattctat ccacttagtg
    27781 ggcaagcttt agattgattg tttttgactg tttcatcagg aattccttga aaaataagtt
    27841 tgaaaccttg gttaaaaagt attttatttc attatctttc acattttcgt aaaaatttga
    27901 ttgatttcct gtatttattt tatgataatc ctttgacagt ttttcctctt ttttaacata
    27961 aaaggcttgc attttttatc gaaaacagtt actattaaaa gtgataagat ttttggagga
    28021 atgaatggaa tggacaactg agcgtcgtta cagactttat caagattgga cgcaagaaga
    28081 aattcaacat ataaaggaaa atatggcaca atctccatgg catactcatt accatgttga
    28141 gccaaaaaca ggacttctca acgacccaaa tggcttttct tactttgatg gcaagtggat
    28201 cctcttttac cagaattttc cttttggtgc agcccacggt ttaaaatctt gggcacagct
    28261 agaaagtgat gatttgattc actttaaaga aactggaatc aaagttttac cagatactcc
    28321 attagatagc cacggtgcct actctggttc tgccatgcaa tttggcgata acttattcct
    28381 attttataca ggaaatgttc gcgataaaaa ctggatccgt cacccatacc agatcggtgc
    28441 tttgatggac aaggagggta agattacaaa gattgacaag atcttgattg accagccagc
    28501 agactctact gaccacttcc gcgatccaca aatttttaac tttcagggtc aatattatgc
    28561 cattgtcggc ggacaagact tggagaaaaa aggtttcgtt cgtctctaca aggctgtcaa
    28621 taacgactac acaaactggc aagcagttgg cgaccttgac tttgctaacg accgtactgc
    28681 ctacatgatg gaatgtccta atttggtctt tgtagaggaa caacctgtcc ttctctactg
    28741 tccacaagga ttggataaga aagttctaga ctacgataat atctttccaa atatgtataa
    28801 gatcggggct tcctttgacc ctaaaaatgc caaaatggta gatgtgtctc aacttcaaaa
    28861 catggattac ggtttcgaag cctatgcaac tcaagccttc aacgctcctg atgggcgtgc
    28921 tctagcagtt agctggcttg gtttgccaga tgtttcttac ccatctgacc gttttgacca
    28981 ccaaggaacc ttctctttgg tcaaggaact cactatcaaa gacgacaagc tctaccagta
    29041 tccagtcgct gctattaagg accttcgtgc ttctgaagaa gccttctcaa accgttccca
    29101 aaccaagaac acttacgaac ttgaactcaa cttggaagct aatagccaga gcgagattgt
    29161 cttacttgct gataaagaag gtaagggact ttcaatcaac tttgaccttg taaacggtca
    29221 agtaacagtg gatcgtagcc aggctggaga acagtatgcc caagaatttg ggacaactcg
    29281 ttcttgccct atcgagaatc aggctactac tgctacaatc ttcatcgata actctgtctt
    29341 tgaaattttc atcaataaag gagaaaaagt attttctggt cgtgtcttcc cacatgcgga
    29401 ccaaaatggt atcctgatta aatctggaaa cccaactgga acttactatg aattagatta
    29461 tggtcgcaaa actaactgat gtcgccaaac ttgcaggcgt cagtcctact accgtttctc
    29521 gggttatcaa taaaaaaggg tatctatctg agaaaaccat ccaaaaagtc aatgaagcca
    29581 tgcgagaatt gggctataaa cccaacaacc tggctcgtag tctgcaagga aaatcagcta
    29641 agttaatcgg cttgattttc cccaatattt ccaatgtttt ctatgcagaa ttgattgata
    29701 aattggaaca ccaactcttc aaaaatggtt acaagaccat catctgcaac agtgaacatg
    29761 attctgagaa ggaacgcgaa tacatcgaaa tgttggaagc caatcaggtg gacggcatca
    29821 tttctggtag tcacaaccta ggaatcgaag actacaatcg tgtgacagcg ccgattattt
    29881 cctttgaccg aaacctatcg ccagacatcc ctgtcgtctc ctctgacaac tatgctggtg
    29941 gggttcttgc tgcccaaacc ttggtcaaga caggtgccca gtctatcatc atgattacag
    30001 ggaatgacaa ttctaattcg ccaaccggac tgcgccacgc tggttttgca tccgtactcc
    30061 caaaagctcc tattatcaat gtttccagtg acttttctcc cgtcagaaaa gaaatggaaa
    30121 tcaagaatat cttgacccgg gaaaaaccag atgccatttt tgcttcggat gatttgacag
    30181 ctattctggt cattaaaatc gctcaagaat tgggcatttc tgtcccaaaa gagctcaagg
    30241 tcatcggcta tgatgggacc tactttatcg aaaattacta ccctcaattg gctactatca
    30301 agcaaccttt ggaagagatt gcttgtctca ctattgatct tctcttgcaa aagattgaag
    30361 gcaaggaagt cgccacaact ggttacttct taccagttac gctattacca ggaaaaagta
    30421 tttaaacaca agaaaactca gaccgattcg tctgagtttt tatgatctta aattttcgag
    30481 atagcgctgg gctgtctcta ggttaaaggt tttatctgag atgaggcgct ctactagggg
    30541 agcaacttca gattcactag ccccagctag gagagctagg gatttggcct gtagtttcat
    30601 gtggccttgc tggatgcccg tacttaccaa ggctttgagg gctgcaaaat tttgagcaag
    30661 accgatggac acgataatct gggctaattc tctggcagaa ggatttccta gtagatcatg
    30721 actgagaact acacgtgggt tgaggccgat agagccaccc ttagtcgcta caggcatggg
    30781 cagggtcatc tcaccgacca attcttctct ttcaaggtcc agcgtccagc agctaagacc
    30841 ttgatagcgt ccatctcgac tggcaaaggc atgggcccca gcttcgatgg cacgccagtc
    30901 attaccagtg gcaatcaaaa tcgcatcaat accattaaaa attcctttat tatgagtagc
    30961 agctcggtaa ggatcagcct gcgcaaactg actagccaac gcaattttct ccgcaatctc
    31021 tcgtccttga tccttttggc ggctcaagta gcgaaaggcg atgcgacagc ttgcagtcac
    31081 cagagaatcg gtcgcgtagt tggacaggat tcccatgaga ctctgtccct gactgagttc
    31141 ttctaagact ggtttcaagg cttccagcat ggtgttgagc atattggcac ccatggcttc
    31201 ctgggtatcg acatgaatat aaacaacgag aaagtctggt tcgcctttta tctgctcgac
    31261 atgcagatca cgcgccccac ctccacgttt aacgatagaa ggataggctt gattggcaag
    31321 ctccaagagc tccgctttct tgctggcaat cttctcttgc gctagtttag gattagcaac
    31381 ttgataaagg gctacctgcc caatcatctg tcgctgatgg acttgtgcag taaaaccacc
    31441 tgcacgcttg atgattttgc tggcatagct ggccgccgca accacagagg gttcttctgt
    31501 cacataggga acggtgtatt cctgaccgtt gacaagtacc tccggaacca gtgaataagg
    31561 cagagaaaaa gttcccacta cattctcact cagctggtct gccacagtca cgctcatctg
    31621 ttcatccttc tccagactag cttgtctctc aggactaagg agcgcctgag cttttaacag
    31681 ctcgaggcgc tcttggtatg attttttaga aaatccattc caacttatct tcattatttt
    31741 tcaaccttgc tataacggcg ttggtggtcg agaatttcaa ccaaggcaaa atcttgattt
    31801 tcatagccag caaactgggc agagttagtt tcatccaagt ttacttcctc aaaaaagacc
    31861 ttttcatagt ctgcaacgga tagggcagtt cgttggttga gcttgttcaa acggtcttta
    31921 tccaaataag cttcatatcc ttcaaccaat tcaccactga agaactcagc cacagctcca
    31981 cttccgtaac tataaagggc gattttatcc ccagctttca agctatctgt attttccaag
    32041 agagacaaaa gtccaaggaa aagtgaacct gtgtagatat tccccacctt ttgactgtag
    32101 agaatagact ggtcaaaatg cttttgtaag aggtcttttt tctcttgagg caggctctta
    32161 tccatgattt ttttcaagcc ttttagcgct aatttaggat aaggcaagtg gaaacaaaca
    32221 gccgcaaaat catccaaagt aagctggtag cgtttttgat attcaagcca agtcgttttc
    32281 aaactatcca agtattgttg ggtagaatag acaccattta cataaggagt tgtcgagtaa
    32341 tttggtcgcc agaaatccat gatgtcacgg gtctgagcta cattgtcatt attaaaggcc
    32401 atcatgcgtg gattttgtgt aatcaacata gctacacttc cagcaccttg agttggttct
    32461 cctggagttt caataccgta tttggcaata tcactggcaa tgaccaagac cttggactcc
    32521 ggagaatttt ccacatgcaa tttggcataa tggagggcag cagtcgctcc gtagcaggct
    32581 tctttaatct cgaaactacg agcaaagggc tggatgccca gcaagccatg cacaaagacg
    32641 gccgcagcct tactctggtc aattcctgac tcggtcgcca caatgaccat gtcaacttct
    32701 tgtctttctt gctcagttaa aatagagtca ctagcactgg ccgccaaggt cacgatatcc
    32761 tcagttaggg gcgcaatact caattccttg agtaagagtc ctttacttaa tttttcaggg
    32821 tcaattcccc tcgcttctgc taagtcttgt aatttcaaga catattgact ggtcgcaaaa
    32881 ccaatcttat caataccgat tgtcatattt acctctgttt tatcattcat gtaaaaaatc
    32941 gttctatact attttatcac aaatggcagt aaaagagaga aaaaagactt gattcaccaa
    33001 atcaagcctc ttattggtca tcattttaaa gaatgattag ttgctagaga gttcaccgat
    33061 ataagtagct ttataagctc cattcacagt tatcagctcc tggaggatca aatttcctga
    33121 gtaagtcctt cccatctcat ctacaaattt ttgataaaac tgactggtcg gaatttctct
    33181 gacatcctta tcaaatgtct tatcaagtgt tttactaacc ttctcagcaa tcaattgatg
    33241 ctcttgccat ccactttgaa actctgagcc cgaactagaa accatgactg ggataaacaa
    33301 caaggtcagt agatttacag acaataagga aagtagtaga cttcctgcaa aactagaatc
    33361 ctagttcatg attgataata ccagcaatca aattcattcg taatccgaag cgtttacgat
    33421 gatttcgata ggttg
//
LOCUS       DAVHEX010000019        32588 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVHEX010000019 DAVHEX010000000
VERSION     DAVHEX010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32588
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.019"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(186..1748)
                     /gene="guaA"
                     /locus_tag="VVL45_000854"
     CDS             complement(186..1748)
                     /gene="guaA"
                     /locus_tag="VVL45_000854"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEU4187053.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVH
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            1890..2588
                     /locus_tag="VVL45_000855"
     CDS             1890..2588
                     /locus_tag="VVL45_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU4187054.1"
                     /translation="MLPAYIKIHDQIKKDIDEYRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(2631..3527)
                     /locus_tag="VVL45_000856"
     CDS             complement(2631..3527)
                     /locus_tag="VVL45_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU4187055.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAIFFAFLIMNEQFYPIDF
                     LGMAMILFAVTLISLKDLFLEK"
     gene            complement(3536..4471)
                     /locus_tag="VVL45_000857"
     CDS             complement(3536..4471)
                     /locus_tag="VVL45_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEU4187056.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(4468..5193)
                     /locus_tag="VVL45_000858"
     CDS             complement(4468..5193)
                     /locus_tag="VVL45_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEU4187057.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDIASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLGKDRNNVELWFEEV"
     gene            complement(5277..5912)
                     /locus_tag="VVL45_000859"
     CDS             complement(5277..5912)
                     /locus_tag="VVL45_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HEU4187058.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEREEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKSLKDYLY"
     gene            complement(5914..6741)
                     /locus_tag="VVL45_000860"
     CDS             complement(5914..6741)
                     /locus_tag="VVL45_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU4187059.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALTHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(6763..7689)
                     /locus_tag="VVL45_000861"
                     /pseudo
     CDS             complement(6763..7689)
                     /locus_tag="VVL45_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809127.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
     gene            8350..8961
                     /locus_tag="VVL45_000862"
     CDS             8350..8961
                     /locus_tag="VVL45_000862"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU4187060.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRAIAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            9006..9734
                     /gene="rlmB"
                     /locus_tag="VVL45_000863"
     CDS             9006..9734
                     /gene="rlmB"
                     /locus_tag="VVL45_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEU4187061.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEITEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRTVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(9773..10684)
                     /gene="trxB"
                     /locus_tag="VVL45_000864"
     CDS             complement(9773..10684)
                     /gene="trxB"
                     /locus_tag="VVL45_000864"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEU4187062.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVREIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDNHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(10750..10974)
                     /locus_tag="VVL45_000865"
     CDS             complement(10750..10974)
                     /locus_tag="VVL45_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEU4187063.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTARARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(11052..11795)
                     /locus_tag="VVL45_000866"
     CDS             complement(11052..11795)
                     /locus_tag="VVL45_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU4187064.1"
                     /translation="MIKISNLSKSFSGQTVLDHLDLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(11795..12595)
                     /locus_tag="VVL45_000867"
     CDS             complement(11795..12595)
                     /locus_tag="VVL45_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU4187065.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(12753..13109)
                     /locus_tag="VVL45_000868"
     CDS             complement(12753..13109)
                     /locus_tag="VVL45_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEU4187066.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(13103..13633)
                     /locus_tag="VVL45_000869"
     CDS             complement(13103..13633)
                     /locus_tag="VVL45_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEU4187067.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDRSNRRRSTC"
     gene            complement(13633..14127)
                     /locus_tag="VVL45_000870"
     CDS             complement(13633..14127)
                     /locus_tag="VVL45_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4187068.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKMPVDGERLAYQ
                     KLKK"
     gene            14262..14708
                     /locus_tag="VVL45_000871"
     CDS             14262..14708
                     /locus_tag="VVL45_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEU4187069.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            14705..15352
                     /locus_tag="VVL45_000872"
     CDS             14705..15352
                     /locus_tag="VVL45_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEU4187070.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
     gene            complement(15373..15954)
                     /gene="pdxT"
                     /locus_tag="VVL45_000873"
     CDS             complement(15373..15954)
                     /gene="pdxT"
                     /locus_tag="VVL45_000873"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEU4187071.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(15955..16830)
                     /gene="pdxS"
                     /locus_tag="VVL45_000874"
     CDS             complement(15955..16830)
                     /gene="pdxS"
                     /locus_tag="VVL45_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEU4187072.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(16982..18361)
                     /locus_tag="VVL45_000875"
     CDS             complement(16982..18361)
                     /locus_tag="VVL45_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU4187073.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALAGGKIELFRNGAF
                     LVDKKQETSIPDVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            18655..19578
                     /locus_tag="VVL45_000876"
     CDS             18655..19578
                     /locus_tag="VVL45_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEU4187074.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGIAPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            19638..20243
                     /locus_tag="VVL45_000877"
     CDS             19638..20243
                     /locus_tag="VVL45_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU4187075.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFTDKAEIELVEIKAIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            20262..21506
                     /locus_tag="VVL45_000878"
     CDS             20262..21506
                     /locus_tag="VVL45_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU4187076.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRYFKLKFELEVLEIDEVPMF
                     NQDEKWDESFQLRYLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AFTGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(21729..21986)
                     /locus_tag="VVL45_000879"
     CDS             complement(21729..21986)
                     /locus_tag="VVL45_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEU4187077.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(22028..24064)
                     /locus_tag="VVL45_000880"
     CDS             complement(22028..24064)
                     /locus_tag="VVL45_000880"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEU4187078.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKGKRLSFEAI
                     QTFSTPRRLAVRVTGLADKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEIGQAVEAIVPGIVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGQETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKASLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLILSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            complement(24276..25193)
                     /gene="glyQ"
                     /locus_tag="VVL45_000881"
     CDS             complement(24276..25193)
                     /gene="glyQ"
                     /locus_tag="VVL45_000881"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEU4187079.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQDCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRAKLLAEDAE"
     gene            complement(25564..26324)
                     /locus_tag="VVL45_000882"
                     /pseudo
     CDS             complement(25564..26324)
                     /locus_tag="VVL45_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
     gene            complement(26597..27439)
                     /locus_tag="VVL45_000883"
     CDS             complement(26597..27439)
                     /locus_tag="VVL45_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU4187080.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQDQVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     VAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERETLKTIAVQSGAPRVD
                     DVDF"
     gene            complement(27553..28944)
                     /locus_tag="VVL45_000884"
     CDS             complement(27553..28944)
                     /locus_tag="VVL45_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU4187081.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKIEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIEQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKHQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            complement(29179..29556)
                     /locus_tag="VVL45_000885"
                     /pseudo
     CDS             complement(29179..29556)
                     /locus_tag="VVL45_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
     gene            29771..30748
                     /locus_tag="VVL45_000886"
     CDS             29771..30748
                     /locus_tag="VVL45_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU4187082.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVSQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLAG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFGKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            30745..31827
                     /locus_tag="VVL45_000887"
     CDS             30745..31827
                     /locus_tag="VVL45_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU4187083.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILAVSLASDVNVKFRKIILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(31891..32444)
                     /locus_tag="VVL45_000888"
                     /pseudo
     CDS             complement(31891..32444)
                     /locus_tag="VVL45_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(32478..>32588)
                     /locus_tag="VVL45_000889"
     CDS             complement(32478..>32588)
                     /locus_tag="VVL45_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEU4187084.1"
                     /translation="QLEQAIIDYIDYYNNKKIKIKLKGLSPVQYRTKSFG"
BASE COUNT         9650 a         7305 c         5861 g         9772 t
ORIGIN      
        1 attctcatta tatatatcaa aagaccgcag cttgaaaact acggtctaat tttttgtcca
       61 aatgtaaacc acttggaact attattcaat ttattctttt tagcaaatgt tcccgtttta
      121 atcccagtta cagtttttaa tcctctgtaa ccgttgattt tacaaagctt ttaatttttt
      181 tgcgattatt cccactcaac tgttgcaggt ggtttacttg taatatcgta gacgatacgg
      241 ttaacatgat ccacttcatt tacgatacgt actgagattt tttgaagtac ttcccatgga
      301 attttggcaa aatcagcagt cataccatcg atagaagtga tagcacggat tgcaatcgtg
      361 tagtcatacg tacgaccgtc acccataaca ccgactgaat gaacgcctgt gttaacagtg
      421 aagtattgcc aaatatcgcg gtcaagtcca gctttagcga tttcttcacg aagaatagcg
      481 tctgattcac gaacggtttc aagtttctct tcagtgattt cacccatgac acggatagca
      541 agtcctggtc ctgggaatgg ttggcgccat acgatatggt ctggcatacc aagctctgta
      601 ccaagagcac gaacttcatc cttgtaaaga gtattgagtg gttcaatcaa ttcaaactgc
      661 atatcttctg gaagaccacc cacgttgtgg tgtgacttga tagtttgagc tgtatccgta
      721 ccagactcga taacatctgt atataaagta ccttgagcaa ggaatttcac atctttgagc
      781 ttgcttgctt cgtcatcgaa tacatagaca aactcgttac cgatgatttt acgtttttgt
      841 tcagggtcag aaacgccagc aagtttgtca aggaaacgtt tagcagcgtc tgctttgacg
      901 atattcaaac caaacttacc accgagcatg tccataactt gatcagcttc gcctttacga
      961 agaagaccgt ggtctacgaa gatacagatc aattgatcgc caatcgcttt ttggagaaga
     1021 accccaacga cagatgagtc aacaccacct gatagaccaa gaaggacacg tttatcaccg
     1081 acggtttcac gaattttttt gatctgcatg tcaatgaaat tatccattga ccagtcacct
     1141 ttagccttac aaatgttaag ggcaaagtta cgaaggatat catttccgta tacagaatga
     1201 cgaacttctg ggtggaattg gataccgtaa atgtgtttat ctgggttttc gatggctgcg
     1261 tatgggcagt cagctgatgt acctgtacga acaaagtcag caggaatctc agtaaccgca
     1321 tcaccatggc tcatcaaaac agtctgttca tcaggtgttg attcaaaaag cgctgatggt
     1381 gtgtgagtta gggttgattg accgtattca cgatttccag catcacctgc aggaacaact
     1441 tttcctccaa gtttatgggt caataactgc ataccataac agattcccaa aattggaatt
     1501 ccgagttcga agatttctgg gtcaatatca aatgaaccat cttcatatac agaatttgga
     1561 ccacctgata gaataattcc tacaggattg acttcacgaa cttcagcagc tgaaatttta
     1621 tggcttttta gttctgaaaa aacaccaatc tcacggatac ggcgtgaaat cagctggttg
     1681 tactggctac catagtccaa tacgatgatt ttttctacat cttgcaaatc agttgaaatg
     1741 ttgctcatct ttttcctttc tcaaaagaaa tatcttttct caatattcta tcacaaataa
     1801 gggcgaatta ccaactaaac aaggcgaagt ttgacttttt ctagtttatt ggggtacaat
     1861 agagaaaaga tagaaatgaa gtgaaaaata tgctacctgc ttatataaaa atccatgatc
     1921 agataaaaaa ggatattgac gagtaccgtt gggctattgg tgagaggctt cccagtgaaa
     1981 gagatttagc tgagcagttt gcggtcagtc gcatgaccct ccgccaagcc gtatctctat
     2041 tagtcgaaga aggcgtctta gagcgccgtg taggaagcgg cacctttgtt tccagtactc
     2101 gagtacaaga aaagatgcga gggacaacca gttttactga aattgtcaaa tcccaaggta
     2161 aagttccctc tagccagctc atttcctaca aaaaaaccat tcccaatgag caagaagttg
     2221 ccaagctagg aatttttcca acggacaata ttatccgaat ggagcgggtc cgctatgccg
     2281 accaagttcc cctagtttat gaagttgctt ctattcctga aaaattcatt aaggacttta
     2341 aaaaagaaga aatcaccagt catttcttcc aaaccttgca aaaacatggc tatcgtatcg
     2401 gcaaatctca acagaccatc tatgctcgcc ttgctaaaga aaagattgcc cactatttgg
     2461 aagttgaaaa aggacatgct attcttggat tgacccaggt ttcctaccta gaagatggta
     2521 ctgcttttga atacgtaaaa agtcaatatg taggcgaacg ctttgaattt tatcttgaaa
     2581 ataattagat taccacgcaa aaactctctg tcacggacaa agagtctttt ttatttttct
     2641 aagaataaat ctttcaaaga aatcaaagtt acagcaaaca atatcattgc cataccaaga
     2701 aaatcaatgg gataaaattg ttcattcatt attaagaagg caaagaaaat cgccgatatt
     2761 ggctcaattg aagccaacaa gcttgacttg accggtccta tcagactggc tcctttaagg
     2821 aaagctgtat aggcaaagac agtcccgata aggataatgc ctgcaaacgc aaggagaaaa
     2881 tcaagactag tcgggatatc ggcctgtaga acccctgtaa aaggaagggc gaccaaacct
     2941 gctatgacca ttcccacacc aatgaccaag ctgctccccc actttttaat caaggctatg
     3001 ggtaaaatga tatacagagc ataagtcaag gcagaaaaga gaccccagaa cagaccagca
     3061 ggtgtcatgg ataactggtc caactgccca tgtgttgcga tcaggaaggt tcctccgatg
     3121 gcgaatatga tggaaactat ctctcccagt gtcggtgcca ccctatcctt gatacagcta
     3181 taaattaaaa ttccgacagg acaaacatac tgaagcaccg tcgctgttcc cgcattggtc
     3241 tcctgaatag cagacagata ggcgaattgg ttgagaaaaa gaccaatcag agcaaaaata
     3301 agaagagaca gcaaactctt tctatccttt aaaaagacca gtattttatc ctttgcagta
     3361 gcataagcca agagcatgag aattccacca gcgattaaaa gacgcaagtt agtcaagacc
     3421 agagccgaaa ttccgtgtgc cattaggtat tggccactcg ttcctgacaa cccccaagca
     3481 ataccagcca caactgttag taaagtccct tttaaactat ttgacatgtc tctccttact
     3541 cttctttgcg aatcaagttc atcacttgat tgcggtctaa tatccattga gctctctcgt
     3601 ccttttcata ggtacgattc gataggaaaa tagtggcttc ttgcttctga cgattccaca
     3661 tgataaaggt acctgtatag cccgtatggt ctagccaatc tccttccaaa ttccatgcta
     3721 aagaacgttc cttgtcatcc aaaggagaaa aattttgatt taagtctctt gcaaaatcat
     3781 ctgctaaata gtgttctaaa aagatttgta aatcctttat agtcgaaaat aaaccagcac
     3841 tcccagcatg tctacccagg agacgagcct tgggatcatg cactatgcct gcctctacac
     3901 ctctaactgt tggaacagca agctcaactg gcccaaactt agtttccgtc attccccaag
     3961 gtttccagac ttgatccttt aaaatcacat ccaaatcttg attaaaaatt ctttccaaaa
     4021 taaagcccaa cagcaaaaaa tggacatccg aataaagaaa ggctggctga cttcgtctgt
     4081 tgagatgaaa catcgcttcc tttaattcag gggctgttaa aagatcacga ttaggaataa
     4141 aaggatcaag gtctgttgca tgagtcaaga gctggcgaat agtgatgtct ggataatcac
     4201 tctcaggtaa aaaatctatt accagtctat caatatctaa ttgacctatt tcccacaaga
     4261 aggtacaaac tgtgccaacc ccaacaacct tgctgacact agctaggtca taaactagtc
     4321 ctgcctcagt ctgcaagcca tgctctgggt cactctggcc taaatagaac tctgtccatt
     4381 gattgtcctt aaaatacgca aaagaggctc cgggataaat ccctgcctcg atttgttctt
     4441 ctattttttt aataatcttg gtccacttca tacttcttca aaccacaatt caacattatt
     4501 tctatcttta ccaaggaaga atttttcaga cttaggaata aaatattcag tagactcaaa
     4561 cttctggcga agacttgcta tgtctaattc attgaccaag aacttgagca tagataagtc
     4621 ccaggtaacc gtattgtcca cagtcaaatc ctgcccctga gctggaataa aatcaaggga
     4681 tgccccaatt tcagatgatt ccaagaaact ttcgatatca gttgggagat gtaattccat
     4741 agaaatctca aatttactta aagaaattga tgccaaatct gtttgaaatt caggcttttc
     4801 tcctacttct actagacttg ctatgtcatc ttccgcatga atcaaaatca aatcatcttc
     4861 tggtgagaaa atttcaaaag cgtagccatt ttgaccttta tataatcgat gaatcgaatc
     4921 tgttttagat aagattcctt caatttctaa gggattttcc accttgacaa tcaatctagc
     4981 tagttttttt cttccctcta ccttacgagt acgcatactg ggagcttctt ctaaaaccag
     5041 cttttcaaga cccgtttggt cacctagtga cagaaaggcc gattcttcta acaaggcctt
     5101 cattccaagg gtttcaatat aaaatgtttc atttaatttt ctattattag cttttaaagt
     5161 aggaataatc cgtacaatct gatttacatt cataaattcc tccgtcactt tttattttaa
     5221 aggattttag aattttttac aagatattat gctcaaattt aacattattt agctatttag
     5281 taaagatagt ctttcaagga ttttgacaaa gcctgataac cagcaatact gaggtgcagt
     5341 ccatcagttg tatattcttt tttgagttgg cctgcttggt ctgtcaaaca atcaaatact
     5401 ggcacaaatt ccacctgcat ataggcagat gcaagctctt gataggcttg attccagttc
     5461 tgaatttttt cattcgagcg gatatagact gcctgctggt actcctctct ctcattgaca
     5521 ggcaaaatgg aaagcaattt aatctctgtc aatggataat cgcgagcaac ggattgaatg
     5581 atagcttcga gattattgag agcctcattc acaggaacat cctttccgat atcatttgtc
     5641 ccaatcagaa gaaaaatttt atctactgct ccaccatata gatgagcatc aaggttctct
     5701 agtaacagtc ctgtctgata gccacgaatt cctcgattga caatcgtctt tgaagtccca
     5761 aatagctcct gtagaggata atactcgaca atggaatccc caataaaaag aatgtttggt
     5821 tctacaacag aaatgtgatt taggtgacga tacttagttt gaattttttc ttgttccttt
     5881 aggagccaat tttctaataa ctgtactgcc acttcattct cctttttcta accagttttc
     5941 caagacttga taaactcctc cttggctgtt ggctggtgct agagcagtcg ccacagcttt
     6001 ggctttctca tcagcatttt ccatcgcata ggcaattcca gccatttcaa gcatttcaac
     6061 atcattttca ctatcaccaa aagccatgat ttcttgggat ttcaagtccc agcgcttgag
     6121 taattcctcc aagccccatg ctttatgaat cccagcttgg aggatatcaa tgcaaccata
     6181 gccactggat acagctcgga cacggccatc aaagagagca ttgatttctt ccaaaaccga
     6241 actcaaacgt tcctcaccaa caaccatgct catcttgagc acaccaccaa agaggtcaga
     6301 tgttaattca tccacaaatt gcatccgttg gtagaatttt tcaatcattt ctggagtcat
     6361 aaaactttca agatctgtaa aaatcgtacc ttccttgaca aaatcaccct tcatccccgt
     6421 tacaacaaac tggtcctgac acgctcgacc cttgaaatga gtcaaagcct tgttgacaat
     6481 ggcgtcatcc catgtctgag cctgaatcaa ttcattgttt tcaaaaatac gagcgccatt
     6541 agcaacaacc agaaccactc gatccaccaa gggactcagt agttgtctca tgcggtgaat
     6601 ttcattgccc gtcgcaatga caaaacgaat gcccctttga tccaactgat ctaaaatctt
     6661 ttccaatcgt gggagatcaa gctgacctct agcatccagc aaggtcccat ccatatctgt
     6721 cgcaattacc ttaatcatca tttactttcc tctggattca agctaatacc acttcaatcc
     6781 cacatgttca ttcatttctt tataggctgt atcaattcgt tccttagtcg cttcatctaa
     6841 ctggtcacga tgactgccat cctctataaa atcttccaaa atataagttt ggtaatgata
     6901 caagggatat ccttctggtg aaaaggttcc ctttggtgtt cgattgaccc aagtaggatg
     6961 agctttagct gttccgatag ttgtttttcc atccttcttc ttgatggtga catccatgag
     7021 aacaccacgt tcagtccact tagcattctc ttcatctccc atagattcaa ttcgttgatt
     7081 ggaaatgaag ttccccattt aatagataat gagtttctta tctccatctt tttcaaccgt
     7141 ttcagatggt tcaacaacgt gaggatgccc tccaaagata atatccgctc ccaaatcgat
     7201 catcttgtga taaagagctt tttgttcttc agttggttcc aatcgatact caacacccat
     7261 ctgaagcatg ataatggtga tatctgcttc cttctctgcc cgttcaattt caaccttcat
     7321 cttatcttcg tttaaatctg aaagataacg attatagtct tcctgagaaa tatactgctc
     7381 aattccattg aaaccatagg aataggctaa caatgcaacc ttgataccat tcacttcctt
     7441 aatgaccagc ggagcctgat cacgtggttc gtgcgtataa actccgattg gagtgattcc
     7501 agctttctca ataatatcgg ccgttgaaat aactccctca atttgcgaat ccaaaatatg
     7561 attatgagct aaatccagca catgataacc tgcctcctta atagcatcca taacttcaac
     7621 aggagcatta aagagaagat aacccgctaa ataatgatcc ttattaatgg ttccttcaaa
     7681 atcaccaata gctaaatctg ctgcttagtc cccttgcttg agccaaggag tcacatactc
     7741 aaaattttca tgaaagtcat aggtaccgtc ttctttttta gctgaaaaga aaagtccatc
     7801 gtggtagagc aaatccccgt gggccataat tctggcagtt ttttcctctt cctactcctg
     7861 agacttttgc ttagtcccct cttgagaaat agtatctcgt ttttgactag tcaagggatt
     7921 ccttggaagc tttcaaacaa caagaccaag cccattgata aaccaactgc tagcaagagt
     7981 atcgccacaa atcccttatt gctccacttg cgataactcc taaaaagttt accaagccct
     8041 tcataaaacg aaaagctaaa ccaccctgat ttcgattttg tcttctttgt atcttcgttc
     8101 tccctacttt cttatgcaag ccttttcttt ttattatatc acagataagt atttctttca
     8161 caattgaatt gaacttccta tctattttct ataaatccta aatgccataa tgctttcaat
     8221 tcttgtcatt ttgtgatatc atgtagaggt aatgaaagga aatgaactaa tccacagtgg
     8281 cttattccaa gtataccact tgggctttgg cagtagctaa ctgcactaaa cataatataa
     8341 ggagagaaaa tgtctgcaat agaacgtatt acaaaagctg ctcacttaat tgatatgaac
     8401 gatattatcc gtgaagggaa tcctactcta cgcgcgattg ctgaggaagt cactttcccc
     8461 ctatctgacc aggaaatcat cctaggcgaa aagatgatgc aattccttaa acattcccaa
     8521 gatcctgtca tggctgaaaa aatgggactc cgcggtggtg ttggactggc tgctccccag
     8581 ttagatatct caaaacgcat tatcgctgtt ttggtaccta atattgttga agaaggcgaa
     8641 actccacagg aagcctacga tttggaagcc attatgtaca atccaaaaat cgtctctcac
     8701 tctgttcaag atgctgctct tggcgaagga gaaggttgcc tgtctgttga ccgtaacgtg
     8761 cctggctatg ttgttcgcca tgcccgcgtt actgttgact actttgacaa agatggagaa
     8821 aaacaccgta tcaaactcaa aggctacaac tccattgttg ttcagcatga aattgaccac
     8881 attaacggta tcatgtttta cgatcgcatc aatgaaaaag acccatttgc agttaaagat
     8941 ggtttactga ttcttgaata aagaaaatcc cgttgcaaga cggggttttg tgttataata
     9001 gaggcatgaa aacaaatgat attgtctatg gtgtccacgc cgttaccgaa gccctccttg
     9061 caaatacagg aaacaaactc tacctccaag aagatctccg aggtaagaat gttgagaaag
     9121 tcaaggaact agctacagaa aagaaggtgt ccatttcttg gacatcaaaa aaatctctct
     9181 ctgagattac tgaaggtgct gttcatcaag gttttgttct acgagtgtct gaatttgcct
     9241 atagcgagct agattacatc cttgcaaaaa cacgccaaga agaaaatcca cttctattga
     9301 ttctagatgg tctaaccgat ccccataatc tgggttctat cttgcgaaca gccgatgcga
     9361 ccaatgtttc aggtgtcatc attcccaagc accgtactgt cggagtaact cctgtcgttg
     9421 ccaaaacagc cacaggtgct attgaacacg ttccaattgc ccgagtgacc aacctcagtc
     9481 aaaccttaga taaacttaag gatgaaggtt tctggacctt tggaacggat atgaacggta
     9541 ctccttgcca caagtggaat acaaaaggga aaatcgccct catcattgga aatgaaggaa
     9601 aaggtatctc tagcaacatc aaaaaacagg tcgatgaaat gattaccatt ccgatgaatg
     9661 gacatgttca aagccttaat gccagtgttg ctgcggccat tctcatgtac gaagttttcc
     9721 gaaatagact ataaaaaagt ttccagtcat ctgattggaa acttttttat gattaactat
     9781 gttctgtaat gaatttatag gcttcttgac cagcgatagc tccatctcca accgctgttg
     9841 ttacttggcg aaggtctttc aagcgaacat ctccaactgc aaagataccg tcgactgcag
     9901 ttttcatgtg gttatctgtc acaatccatc ctgcctgatc ttggatattc aattctttaa
     9961 caaaatcgct aagagggtcc aaaccaacat agataaagac accaccgaag gcttgttctg
    10021 tcacttgacc tgttttcaca ttttcaaata cgactgattc tactcggttt tcacccttga
    10081 tttcccttac tacagaatcc cagataaagc tgattttttc attcgcaaag gcgcgatctt
    10141 gtaaaacctt ttgggcacga agttggtcac gacggtgaac aatggtaaca gtcttagcaa
    10201 aacgagtcaa gaagagggct tcttcaacag ctgaatctcc accaccaact accaataaat
    10261 cttggtcacg gaagaaagca ccatcacaca cagcacagta agaaacacca cgactgttca
    10321 gttcttcttc tccaggcact cccaaaggac ggtgtttaga accagttgct acgataactg
    10381 tacgtgtttc atatgtttgg tcatcagtca tcactttctt aaaatcacca tggtcttcga
    10441 cattttcaac ataaccataa atgtgctcaa caccaagatt ttcaagtggt tcaaacatct
    10501 tttcagccaa ttcaggtcca ctaatattag cgtatcctgg gtaattttcg atatcagatg
    10561 tattattcat ctgaccacct ggcagaccac cttcaatcaa agctactttt agattgcttc
    10621 gagcagcata caaggccgca gtcatccctg caggtccagc accgataata atagtatcgt
    10681 acatatagat tccttctttc ttgttgtaac tatctttatt ctaactcttt cttgtcaatc
    10741 acgcaaggat tatgccttga aaacaagaat taaactcata accgcaaagg ataatactgg
    10801 gacaaccaag actccaatca taatcatatc atagagatgg gcttggcgag ccctggctgt
    10861 cttatcaact cccgacatgg ctctcagacc aatccaaatc cctaaaaaaa tcagaacaag
    10921 gatggtggtc aagatcaaac tctcgaaata taaagaaaat agttgcagta gcatgatttc
    10981 tctcatttct atctttttta aagagtaaac tcagctagtc caactaactg agttttcctt
    11041 tatctattat atcaaatata agtccgtttg taactagcga agaattcttt tgtccgctct
    11101 tctttagggg tgtggataat ctcatccgga gttccagact cgatgatttt ccccttatct
    11161 aagaagagaa ttttatccgc aacttgggct acaaaggaca tgtcatgact gaccaaaatc
    11221 atggtctgac ctgacttagc agcatctgca atagactttt ctacttcacc gaccaattct
    11281 gggtcaaggg ctgaagttgg ttcgtctaag agcaaaacat ctggtttcat agcaagcgca
    11341 cgcgctaggg caacccgttg cttctgtcca cctgataaat ggcgaggata atggttttca
    11401 cggtccgaaa gcccaacctt agccaactct tccttggcaa tcttagtcgc ttcttggtca
    11461 gataatttct tgacaacaac caagccttct ttcacattat caagtgctgt tcggcgttca
    11521 aacaaattaa actgttggaa aaccatagac aacttacgac gtagggcaag gatttcttct
    11581 tgagtgattt tagaaaaatc aactgaaaaa ccatcaatct gaatagagcc actgtcaggt
    11641 gtttcaagat aattgagact gcgaagaaag gttgattttc cagctcctga agaaccaatc
    11701 aaggctacaa cttccccttt ttgaatatcc aagtccagat gatccaagac agtctgtcct
    11761 gaaaaggatt tgcttaaatt cgaaatctta atcattaacg aaggtctcct ttcacatctg
    11821 tttgcactgt atcaggtgca gaaatagcca tttttctctc gatgaaacga ccgaggcttt
    11881 caattccgat attgactacc caataaacaa gggcaacgga gatgaagcgt tcaaaatagc
    11941 gataatcagc tccacctaga atctgagctt gggcaaagac ttccacaaca cccgcactaa
    12001 aagctagaga tgttcccttg gtcaaaccga tgagggaatt aatcaaggtt ggagtagcta
    12061 ccaccgctgc attaggaata atcactcgtc gataaacttg cgctcgggtc atacccagac
    12121 tgcgtgccgc ctcaatctca ccaggattaa ctgagagaat ggctgcacga atggtttcac
    12181 tagcataagc tgcctcatta aaggcaaagg cgacaatcgc aaaagctgca gctggaatcg
    12241 cattgatatt gagaccagtt ccccattgct gattgagggc tttcaaagcc aaagggattc
    12301 cgtagtaggt caacatgagt tgcaccaaaa tcggtgtccc ttttaagaaa ctaacaaaga
    12361 aggcctgcaa gggatataaa atcttgacac gattgatctt cacaatggca aaaagaagcg
    12421 ccaaaaccaa gccaaaaagg gcaccaccaa ttgtcaacat aattgttgtt ggaagttgtt
    12481 ggacaattct agggattcca tcaaagaccg aacgtaggct aaacagcttg ccatccggaa
    12541 tcaattgcat caagttttgg taccaatctg atgctaaaat cgttgtaaca ttcataaaaa
    12601 catcctcttc tgataatgat tcattctacc atgcttctgc cgtcaatgaa attatttgct
    12661 acggtcagat acagactttg tctacctact agtttatcat actttgaaac agggaagtta
    12721 tatattttat ctatcaaaga gatagataaa aactatttca gtcccagttc ctcataagat
    12781 tttcgataac cgatttgctt aacagttcca ttttctacta aaatgggccg ttttaacaac
    12841 ataccgtcac ttgctagcaa ctcagccgct tcttggtttg acaaacttcc taccttatct
    12901 tttagcccta attcacggta tttgatacca ctggtgttga aaaattgctt caattcaaat
    12961 cctgaggttt ctagccaatt caaaatgact tcttggctag gtgtttcttc cacgatatgg
    13021 acggctttat agtccacacc taattgattt aattcttgtt ttgctttttt acaagttgaa
    13081 catttggggt attcgataaa ttctaacatg tgcttctcct tctattgctt ctatctttaa
    13141 aatctactcc ttcatgctcc aagagccaag ctttcttttc cactcctgca gcataacctg
    13201 tcagacgctt gcctgctccc aacacacgat gacaaggtac taggatagac caaggattgc
    13261 gtcccactgc tccaccaatt gcttgagcag aagccacttg caggtcttga gcaatttgtc
    13321 cataggtcac tgtctgacca taaggaatgc cctgtaaata gtcccaaact ctcttttcaa
    13381 aattcgttcc gattggcgcc aagagcaagt tggataaatc ctgaggcttg cctttaaagt
    13441 aatcatctaa gcaagcaata actgggtcta aaataggatg actaacaact tcttctatcg
    13501 tttcatctcc tagtcccctc tcaaaatgct tctgctcctg aacccaaatt ccatacaaat
    13561 aatggtcatc agctacaagt gatagaatac caattggtga ggagtagagg atttttacat
    13621 acttcttttg cattatttct ttaatttttg ataggccaag cgttcgccat ctactggcat
    13681 cttaccgact tgtttaaaac caagtttttc aaaaatatgt tgcataacct tgttttcagc
    13741 atgcgtatct gagcgaaaat caagataatc aaaaccttca atcaagccct ctaagaaggt
    13801 ttgagcaact ccttttccct gcacatctgc tgccacagca atacggtgaa agactagata
    13861 ctctgactct ccagcttgcc agtttccctc ataaatagct tcataggcct cctctggact
    13921 cttggtcaca gcagcatagg ctagcagttc tccctcttcc aaggctacgt aggcttgccc
    13981 tgagatgata tcatcgataa taacatctgc atttggatag ccattttgcc actggtcact
    14041 accagcatct gctaaacatt ttttggcatc ctccatcacc tgcatgatgg catctacttc
    14101 atttggaaaa gctaaacgaa tttccatctt ttttcctcat ttctgactag tttctcccat
    14161 catagcataa tttaagtatt tctacaagca gttaaaactt gactttgtgt ttttgttatt
    14221 ttataatcta gttatcaaaa ctatataaaa aggagttaaa aatgaaaact accttttcct
    14281 acccaaaatg ggcagaaatt ccaaacattg acctctatct ggaccaggtt ttgctctatg
    14341 tcaatcaggt ctgcgcccct atctctccta ataaagacaa gggcctaaca gcatctatgg
    14401 tcaacaatta tgtgaaaaat ggttacctga caaagcctga caaaaaaaaa taccaacgcc
    14461 aacagattgc ccgtttgatt gctatcacaa ccctcaagtc tgtattttct attcaagaaa
    14521 tagcccagac acttaatact ctacaaactc aagcaagttc agaccaactc tacgatgctt
    14581 ttgtggacta catgaaccaa ggaattgacc cagctaaccc tattatccaa accagctgcc
    14641 aaaccgttaa actctatcat caaactctag acttaatcga tcatactcaa gaggaggtaa
    14701 tccaatgaac actagtctta aactcagcaa acaactcagt tttggagagg agattgctaa
    14761 tagcgtgacc catgctgtgg gtgcagtcat catgcttatc ttgctgccta tttcatccat
    14821 ctatagttat gaagcacacg gatttttatc ctctatcggc gtttccattt tcgtcatcag
    14881 tctctttctc atgttcctat catccaccat ttatcactct atggcctatg gttcgaccca
    14941 caaatatgtt ttgcgaatca ttgaccattc tatgatttac gttgccattg ccggctcata
    15001 cacgcccgtt gtcttgacct tgatgaataa ctggtttggc tatctgatta ttgtcatcca
    15061 atggggaacg accatctttg gtattctcta taaaatcttt gctaaaaagg tcaatgagaa
    15121 atttagcctt gctctttacc tgattatggg ctggttggtt ctggctatca ttcctgccat
    15181 tatcagtcaa acgacacccg ttttctggag tctcatggta actggcggac tctgttatac
    15241 agttggagct ggattttatg ccaagaaaaa accttatttc cacatgattt ggcatctctt
    15301 tatcctagct gcgtccgcac ttcaatacat cgctattgtt tattacatgt aaaaaagttg
    15361 agaaattcaa tctcaacttt tttctttaca catattgata aagtactggt gcaagcgcac
    15421 atcatcagtc aattctggat gaaaagaact taccaacata tttttttctt gggctgcaac
    15481 aatttgattg ttcactgttg ctaaaatttc tacaccctca ccaacactac tgataatcgg
    15541 accacggata aaggtcattg gaatcttgcc aactccctta cattctgctt ccgtgtagaa
    15601 acttcctaat tggcgcccat aagcattacg ctcgaccacc atatccatag ttcctagatg
    15661 actctctttc tgagaagtga tttccttagc cagcaaaatt aagcccgcac aggtcccaaa
    15721 cactggtaag ccagatagaa tggcttctcg gatgggaagt agcatgttct ggtcacgtaa
    15781 gagcttgccc atggttgtag actcaccacc aggcaaaatc aaacccgaca agtcactctg
    15841 atcttgctga aaatcatcta gatttctgag ttctacactc tcgacaccta attgatctag
    15901 cacttttgca tgttctgcaa aggccccttg caaggccaat attccgattt tcatctattt
    15961 tcctcgttca gccatgagaa tttggatttc attttcatta ataccaacca tggcttctcc
    16021 taaatcttca gagatttgag ctaggatttg aggattacgg aagttagtca cagccttaac
    16081 aatggcactc gctcgtttaa caggatctcc tgacttgaaa atacctgaac cgacaaagac
    16141 cccctctgcc cctaattgca tcattaacgc agcatctgct ggcgttgcaa cacctccagc
    16201 agcgaaattt acaaccggca attttccatg ttcatgaaca tattggacca attctacagg
    16261 gacttgcaaa tccttggcag caacataaag ctcgtcctca cgtaagtttt gaatgcggcg
    16321 aatttcctga ttcatcatac gcatatgacg aacagcttgg acgatatccc ctgtccctgg
    16381 ttctcctttg gtacgaatca tggaagcacc ttcagcgata cgacgcaagg cttcacccaa
    16441 atccttagca ccacagacaa aaggaacttg gaattctttc ttgtccacat ggaaacggtc
    16501 atcagctgga gatagaactt cactctcgtc gatataatca atttcaatag cctctaaaat
    16561 ctgagcttca acaaaatgcc cgattctgac cttagccatt actggaatac taaccgcttc
    16621 ttggatttcc ttaatcatct ttgggtcgct catgcgggaa actcctccag ctgcacgaat
    16681 atcagccgga attcgttcca aggccatcac agctgccgca ccagcagctt ctgcgatacg
    16741 agcctgttca ggattctgca catccataat aacaccaccc ttgagcatct gtgccaagtt
    16801 tttatttagt tcataacgat tttcagtcat ttgttttatc ctcatcattt gtattggtag
    16861 ctaccttttc attttaagtc agaacagaat gaagcacaag ataaaaaaac tataattgta
    16921 tggggacaaa ttagctaaaa actatctgac cttaacttaa ggccagatag ctcattcatt
    16981 tttatttttc agccgtaagg gcagccattg tgatgtagtt gtatggtttg ttgaagtgtg
    17041 gcaagaagaa gaggtctgtc aatgccaatt tatcaattgt cacatgctct tggatagcaa
    17101 gtgagaacat gtggattccc atgctaattg caatatcatg tgaaaccatt tgggcaccaa
    17161 gaatttcacg gctatctttg tcaaagacaa tcttaattgc tacttcatgg ttgtcatgtt
    17221 tcatgaattc tggtttttga agatcgttaa agcctgtttc agttgcgttg taaccagcag
    17281 ctttcgcttt ttcaagagtc aaaccagttg aaaccatgtg aagaccgtag attgagatac
    17341 catttgaacc ttgaacaccg attccttcca attcatgtcc acaagcattg taggcaccaa
    17401 cgataccagt gcgaacagca tttgaagcaa gagcgatata gcttgtatct ttacgagcat
    17461 tgtcataaac agtcgcacag tcaccaacag cgtaaacgtc tgggatagat gtttcttgtt
    17521 tcttgtctac aaggaaggca ccgttgcgga agagttcgat cttaccacct gcaagggctg
    17581 tgtttggacg gaaaccaact gcaaggataa ccatatccac gtcaaagctt tctttgtcag
    17641 taatcaagcg ttcaacttta ccgtcacctt cgattgcttt aacagtttga cctagagcca
    17701 agcggatgtt gtgatcttcc aagttcttcg ccatcatttg tgtgaagtct ttgtcatagt
    17761 aaccgttcaa gacagtatca acgatatcaa caaggacaac ttcttttcca agacgttcaa
    17821 aggcttcagc aagttcaaca ccgatgtaac caccaccaac aacggcgata cggtcgaggt
    17881 gttggctctt gtcagaaagt ttattgataa cttcttcagc attttggtac aatttcacga
    17941 attgtacgtt ttcaagagtt gctttaaatt cgcggtttcc tttaacaatt tcaacacctt
    18001 cgattggtgg caagattggt gtagagcctg tagcgaaaat caatttttcg tatgattctt
    18061 tgtgctcttt tccttcaact tccgctgtaa ctactttgtt atcatagtcg attgaaagaa
    18121 caggtgagtt catgtaaact ttagcacctt tagcttccaa tttttcttta tcagaataga
    18181 acaagccttc agcaccgtca atttgttcac caatccaaag agccattcca catcctagga
    18241 aagagatgtt agagttttgg tcaaatacaa caatttcgtt ctcatttcca aaattatcca
    18301 acatggtatt gatacatgct gtaccagcgt ggttagcacc gactacaacg attttactca
    18361 taaaaatttc ctgccttcat tctaaattta ccttactagt ataccattta ccgttagcgt
    18421 ttacaagggg ggtactatta tttcagcatt ttttatcaaa aataattccc catttatcct
    18481 gttttcaagc ctgatgaatt ttatgagcat atttcttgac tttttccaaa ttttgtttgt
    18541 gaaaattcta actttatggt attataataa catgaaaatg aaatgtaagg gcttcaattt
    18601 agatgattat ttgatattac aacttatttt ctataagaga aaggagttgg tagcttgact
    18661 cttcgttcac attctgaacg gctaatgggg actactatca ctatttcatt agtggatgag
    18721 caagccgata tctttctcca aaaatccttt gacttgctca aagaacttga ataccgcttc
    18781 aacgccaata gtcaagaatc tgagttgatg gaaatcaatt atcaagcagg aatagccccc
    18841 gtcacggttc atccagacct gtttgagctg atttcacttg ggttagagca tagcctagcg
    18901 ctctctagcc acctcaatat cagcattggt cccttaattc aaacctggcg tatcggtttt
    18961 tcagatgcca aggtcgccca gcctcaagaa attgaatcgg tgctgcctct aatcaatcct
    19021 catggtatcg agttagattc ttctacttct actgtgtttt taaaacagaa aggaatgaag
    19081 atcgatctag gttgtttagc caaaggatac agtgcggata aggttgccca atttcttagg
    19141 aaagaggggg tgacttctgc cttgatcaat ctgggaggga atatcctgac cattggaaaa
    19201 aatcaggcaa gaggggataa cccatggcaa atcgggattc aagacccagc caatcctagg
    19261 ggaaatcatt taatgaccat ccctgttgtc aataaatctg tcgtgacttc aggcatttat
    19321 gaacgtcacc tgaccgtcga tggacaagat taccatcaca tttttgacag tcaaacagga
    19381 tatcctgttg aaacggaact agcgagtcta acaatcatct ctgataaatc agtcgatggc
    19441 gaaatctgga cgactcgact ctttggagaa agacctgctt ctatcctctg gcaagtcgaa
    19501 agtttggagg gcatcgaagt catcctcatc gataaagaag gccacctaag ctgttcttca
    19561 ggaattccta ctctatagag acaactgttt caatggaatt ttaaaattgt attatgtaaa
    19621 aagagaaagg aaatctcatg ttaaaactta ttgctattgt tggaacaaat tcaaaacgtt
    19681 ctacaaaccg tcaattgctt caatacatgc aaaaacactt tactgacaaa gctgaaattg
    19741 aacttgttga aatcaaggcc attcctgtct tcaacaaacc agctgacaag caagtacctg
    19801 ctgaaatatt ggaaattgct gctaaaatcg aagaggcaga tggcgttatt atcggtactc
    19861 ctgagtatga tcactctatt ccagctgttt tgatgagcgc tcttgcttgg ttgtcttatg
    19921 gtatttaccc acttttgaac aaaccaatca tgattacagg tgcttcttac ggtacgcttg
    19981 gttcatctcg tgcccaattg caacttcgtc aaatcttgaa tgctcctgaa atcaaggcaa
    20041 atgttcttcc agatgaattc ttgctctcac actctcttca agcatttaac ccaagtggcg
    20101 acttggttga ccttgatgtt atcaagaaat tggatgccat ctttgatgac ttccgtatct
    20161 ttgtaaaaat cacagaaaaa ttacgtaatg cacaagaatt acttcgcaaa gatgctgaag
    20221 actttgactg ggaaaatttg taagatagga gaccaaaaag aatgaaattt gttggacttg
    20281 ttggatcaaa ctacgatcaa tcatataacc gtaagctctt ggaatttatc cgtcgctact
    20341 tcaaactcaa atttgaatta gaagttcttg aaattgacga agttccaatg tttaaccaag
    20401 atgaaaaatg ggatgaaagt ttccaattac gttatttata taacaaaatt actcgtgcag
    20461 atggtgttat cattgctact cctgagcata accacactat ctcagcttca ctcaaatctg
    20521 tacttgaatg gctttcatac gaagttcatc catttgaaaa caagcctgtt atgattgtgg
    20581 gtgcatcata ctatgaccaa ggaacatcac gtgctcaagt tcaccttcgt aaaatccttg
    20641 atgctccagg tgttaatgcc tacacgcttc caggtaacga attccttctt ggtaaagcta
    20701 aggaagcttt tgataacaac ggaaatatca ccaacgaagg cactgttaaa ttccttgaaa
    20761 cttgcttaga taactttgtt aaatacgtag gagtcgtttc gaaattgaaa aaaccaaaac
    20821 caatcgaatc agaagacttg gattgtggaa aaccaatcgc tacaaccatc acagaagttg
    20881 accctgacga tccagaatgg gtagaaaaag ttgcagcatt cactggtgct gtttctggtg
    20941 atacctatgt caaattggac cacggtatcc taacagttaa ccaaattgat atgttcttga
    21001 aagctatgcc atttgaattg acatacgctg acgacaacaa ccaattcctc tactacaaca
    21061 acgctcacca agatccagac actatgttcg ctaaacgtgt accacctcaa tcaggtagcc
    21121 gtatgtcgac tgttcatggt tctcttccac cagcacgtat gaagaacgta gagtgggtta
    21181 tcggaacact tcgcaacgga aaccaagaat acgtccgtac gatcgttcca ggttctcctg
    21241 caggtgttat caacactcac aactaccaag caatgtacta tcctgatgga tcatacgctg
    21301 gtatcaatga aatcgtcttt aacttccaac catggcttga ctggtaccta aaagagactg
    21361 gtcaacgttt ggtgggtggt agcggaccat ttgctcctgc tgccggaggt catggagacg
    21421 ccgatgctac ttctggcgct tctgattcag gggatgctgg aggccacggt ggtgacgcag
    21481 acgctacagc cggtgcaagt tactaatgat agtttttagg aaccattatg gttccttttt
    21541 tagcgaaaaa agactagcaa acacaaactg ctagtctctt tgttatcgtt tcatctatct
    21601 ttgttaaaag aaagaataca tatactcaat gaaaatcaaa gagcaaacta ggaagctagc
    21661 cgcaggctgt acttaagtac ggcaaggtga agctgacgtg gtttgaattt gattttcgaa
    21721 gagtattatt atgaatttgg atcatcaaga ctacggccat gtaatccttt ttcacgttgt
    21781 acttggcgta gtttttctgg tgtaacatcg tttccttctt cgtccacaat tttgattcct
    21841 tcaatgtggt gacgaacagc gcggcgataa ccttcgatgt actcctcacg tagtttggct
    21901 tgttccactt tttcttctgg tgttaagcct tctgtttttt tctttttagc aagctcattg
    21961 atacgagcaa tttttttcgg atccatttct tctcctttgt aataagataa agtccgttta
    22021 tcaaatttta tttagtgtta atttggttaa aacaagcaaa cttagctgct ttcttggtta
    22081 gttgtgacaa gattgccaaa cgattttgac ggacagcctg atcttcagcc attaccatag
    22141 tattttcaaa gaaagcatca atgactgggc taagcgcaaa aagttgtttc aattgctgac
    22201 ttgcaggtcc tgataaaatg agtgtttcta ctgcttctgc caaagctttt tcttggtcat
    22261 tctcaaatag tgctgaatca accgtagcaa ccccttctgc cttctcggcc aggttaaagg
    22321 cacgagaaag tgattcaaca gatggtttaa aatcttcttc cttgcttact tctacgagag
    22381 cacttgctgc ttccaacata tctgccacaa caaagtttga acctgcaaga actgcttcct
    22441 tgatatcttt tggagtagag cccatcatct tatcaacacg agccttgata aagtccataa
    22501 cctctgcttt attttcataa gtcaaactgt caaatttcaa tgcataaagg ctatcaatca
    22561 gctcatccat agcaatgtgc caaccaaagg catccaagat acgaaccaca ccttgagttg
    22621 cacgacgaag ggcataaggg tcattagaac ctgatggaat caatcctact gagaagaaac
    22681 tcaaaatcgt atccaatttg tctgcaatgg ctagaactgc gccgaccttg ctctctggaa
    22741 gttctccttc agctgatgta ggcatgtagt gttcacgaat agcagctgcc accgctggag
    22801 tttcaccagc aagaagggtg tatttttcac ccataattcc ttggagttcg tcaaattcac
    22861 caaccatacc tgtcaacaag tcaaacttgt aaatggctgc tgcacgagca aggtcaactg
    22921 tttcatccac tgacaaacta gctttttctg ccaaaagtac agtgatttga cccgtacgaa
    22981 tcatgtgttc acgaagagaa ccaatcttct catggaaggt gacattgttt aatttttcaa
    23041 caagatctga aatcaccaat ttttggtctt cacgccagaa gaattctccg tcttccaagc
    23101 gggctaccaa gactttttca tttcctttga tgacattttt caaacgctct gcgtttccgt
    23161 tacgaacaga aatgaagttt ggcaagagtt ttccatcttg atcacgaaca acaaagtaac
    23221 gctggtgttc cttcatagaa gtcaccaaga cttcttctgg aacttcaagg tatttagcat
    23281 caaaacttcc catgaaggca gttgggtatt caaccaaatt caagacttca ttcagcaaat
    23341 ccgcatcaat ttcgatacgt acaccatgtt ttgcctcaat ttccttgatt tggtcaacaa
    23401 tcatttgctc acgttcacat ggatctgcga ttacaaactg cttacgaagg tcttcttcat
    23461 agctcaatgc tgactgaatc ttggtttctt gtcccaaaaa acgatggcca cgactcacac
    23521 gacttccctt gatatcaagg aaatccaagt caaactcttg ctcatccaag agaacagtta
    23581 aagtgtgaac agggcggatg tattcaaagc tatttcccgc ccagtgcatg ctgacaggga
    23641 aagtcagtga cttcaagaca tccacaatgc ctggaacaat ggcttcaact gcttgaccaa
    23701 tttcttcctt agtgacatag acatattctt cacccttgat ttcacggaat tcgatatctt
    23761 caacagtcaa acctttccca cggacaaatc cttgagctgc tttggtgaag tttccatcac
    23821 tatctaaggc aattttcttt gctggaccct tgaaatcttc tgttaaatca gactgtttgt
    23881 ctgcaagacc agttacacga acagccaaac gacgtggtgt tgagaaagtt tgaatggctt
    23941 caaaagacag gcgttttccc ttgaggaagg ctgccatttt ttcgcctagt tgtttttcac
    24001 ttggcgtaac aacataggct ggtaattctt caagaccgag ttctactaat aagttttttg
    24061 tcatgttttt tctccgaaaa gtatcttttt attttccagt ttgccttatg tgattggcac
    24121 gcaagcaacc aacttcgttg tttcattgct aaaattggag ccaaaagtct ccaattttgc
    24181 ctagtatcct tagtttcact aaggatacct tcatcactgc ggcaactggc tcagggctca
    24241 ctctgttcgc ccattttcgt aatttgtcac tctctttatt ctgcgtcttc tgctaggagt
    24301 ttagctcttg tttcttcatc caaaagtggg tagcctaggc gtttgcgttc tgcgacaaag
    24361 gttttggcta cgacacgggc caagttacgg atacgagcga tatagcctgc acgctctgtt
    24421 acagatacgg caccacgcgc gtcaagcaga ttaaaggtat gtgaacattt gagaacatag
    24481 tcataggcag ggtgtaccaa gccttcttct aatgcacgac cagcttcttt ttcaaactta
    24541 tcaaagtttt caagcaacat ttcttggtcc gaaatttcaa atgaatattt tgagtgctca
    24601 tactcaggct ggataaagat ttctccgtat tttacaccat cagcccactc gatatcatag
    24661 acagagtcta cttcttgaat gtaagaagcc aagcgctcca aaccataggt aacttccgca
    24721 gtcacagggc cagttgccaa tccaccgact tgttggaaat aagtgaactg agtgatttcc
    24781 attccgtcaa gccaaacttc ccaaccaaga ccagctgaac cagttgatgg gttttcccag
    24841 ttgtcctcaa caaaacgaat atcgtgctcc aaaggattga ttcccaattt ttccaaagac
    24901 tcaaggtaaa gttcttggat atttgatgga gaaggcttca tgaccacctg gaattggtgg
    24961 tgttggtaga gacggttagg gttttcccca taacgaccgt cagcaggacg acgtgatggc
    25021 tctacataag ctgcattcca tggctcaggt ccgatagcac gaaggaaagt gtaaggactc
    25081 attgtccccg cacctttttc attatcataa gcctgcataa gcatacaatc ttggtcattc
    25141 caaaattgtt gcaaagtcaa aataatttct tgaaatgtta atttcttaga cattgaatac
    25201 tccttaataa aaaataattt cttgcgacac gagtctgcct cgtcgattat acgaggcaag
    25261 acgagttagt actgcgtcta gctcaggcac tctagtgctg agcgtagggc agtccgactg
    25321 taaggaggtt tctgccactg acgcagtgaa atgttaattt cttagacatt gaatactcct
    25381 taataaaaaa taattacttg cgacacgagt ctgcctcgtc gatcatacga ggcaagacga
    25441 gttagtactg cgtctagctc aggcactcta gtgctgagcg tggagcagcc cgactgtaag
    25501 gaggtctctg ccactgatgc agtgaaatgt taatttctta gacattgttt actcctttag
    25561 tttttatatt tctttttcaa ttaatttctg ctgaacaaac caatctaaca agggaaaagt
    25621 tttttctgta tttgcccata aatgataaga atctaagacc gcttgtacac tacctaaatt
    25681 ttctaccaac ttatctactg tcaaaaagct gcgccagtat tcataaaaaa tacttgcata
    25741 gttcttatca taagtcgaag aaccaaaatc attcaatgaa tgccaactat acttcttctg
    25801 aaaaagttct acgagagatt gattacaaat tttttccgct tgaaattcct cttctgtcaa
    25861 gaaatacttg cgactaatat attcaaccat cccctcttcg aaccagatat aagagtcata
    25921 accatcaaaa tcatctaaaa aatgtccgac caatgagcta actcatgtcc tacaatctgt
    25981 aagaggaaat tttcagataa agaatgatag tgactttcta ttgcttgagt ttgctcagaa
    26041 cactcgtagt ccatcaactg atacaaatac aattctttcc aaacagctaa atcaggcgtc
    26101 ataaccactc gtctattatt tgtataggtt ggaacagcac tttccctaat aatctgtgta
    26161 gcagcattaa aatctgacca aataacagct tgcggtaaat cacagactgc aagttcattc
    26221 tttaaaaaag attgataatc tttcaacttt tcagtatttc ttactacaaa atcacgaaat
    26281 gtagctagtt gactatcgtc ttttacaaga taaaggttgt ccacggctct cctttctttc
    26341 acacaataga ctgaataagc aaaagaacca catctgacaa tcctaggttg atttaaccta
    26401 ggggcgtcag aaacgcggtt ccaccctaat ttattacttc attgtttttg aaaatactac
    26461 agaaagcgcc agccctttat tttgccctac ttggctccca ctatcccaag ctcgcttgaa
    26521 gaacgccata agactttctt tcctgctgac tattatatca aaaattagaa taatgtacaa
    26581 ttctttttat gattttctag aaatccacat catcaactcg tggagcaccc gattgaacag
    26641 caatcgtttt taaggtttct ctctcctcat gactcagttc aattccaaag caatcaagat
    26701 tagcttgaat acgagaggtt gtgacagatt ttggaagtgg taaaaatcct tctgccaagc
    26761 tccaggccaa ggctatctga gcaaccgatt ttccgtgatt tgctgctatt tcttggactt
    26821 gcttgctatc aaacagttct ccttgtccaa aaggccccca agcttccaat aaaattccct
    26881 tttcacgaca gtaagctacg acttgatctt gatacacacc tggcgccaag cgaacttgat
    26941 tgaccgcagg aacgatagtt gcagtttcaa gcaaggcatc caaatgatgg ggaagaaaat
    27001 tgctaacgcc gatagcacgg attttccctt cttgatagag gtcttccatc gctctccaaa
    27061 cttccgcatt gcgagttttc catgcgtcat tttctctgag cggttttggg ttcggccaat
    27121 gaatcaaata caaatccaaa taatccaagc ccagtttttc tatagatttt tccaaagctt
    27181 gacgagtttg ctcataggtt tgctgactat tccaaagctt ggtagttacg aacatttctt
    27241 cacgtggaac tccgctatct ttgattgctt gaccaacact ttcttcattc tgataaatcg
    27301 ccgccgtatc aatatgacga taaccagcct tcaaggcttc taacactgca cgataggctt
    27361 cttctccatc cttagcctta aaagttccaa atcccaatac tggaatttct actccattat
    27421 ttaattgata ggtattcata tgcttctcct tctattttat tcatcaatta tagcataaaa
    27481 cattttttaa gtagtactat attctgtaaa aaataagtct gtcaaatatc taacagacct
    27541 attttttctt gcttattcat cacgactata gtacagctca tgagctttta ggcgagtatt
    27601 gagcatctct ggtatggtca caaaggtata accttgattt ttcaaatact caatgaccct
    27661 tggcaaggca ttgactgtcg gactgtgaat atcatgcatc aaaacgatag agccattagc
    27721 tacttgatgc tgaatttctg tcaaaataga tgcttcattt ttactcttcc agtccagact
    27781 atccacatcc cacatgataa agctcaaatc caagctattg cgaatatcat ctgtaatagc
    27841 accataaggt ggacgcatga gtttagaact agaacccagc actttagtta gcacatcctc
    27901 agtatcagta atctgctttt tagcttcatc aagagagagt tgcgagagaa tcggatggct
    27961 ccagctatgg tttccaacaa catgaccttc agattttatc ctcttcacca agtcctcatt
    28021 cccagaaaca tttttcccaa gcacaaagaa agtcgcttta atatcatatt tagctagggt
    28081 ctctaatacc tgcggggtcg ttgctggatt tggaccatca tcaaaggtta gagcgacaac
    28141 tttttgatgt ttcttatcaa agtaagattg gtacaaggcc gcatcttttt cgagtaagta
    28201 cgaagattgg ataacatcaa agaaagcaga tactggcaag gctatctctt ctaaattttc
    28261 aaccacagga cttggataaa ggataatctg actatccttg taatcaaaat tccatgcaga
    28321 caagtcttgg tcagagaagt tttttacaat ctgctcactc tggtcttgct ctattttttt
    28381 atcctctatg aaggaggtca actcttttat cagctgttcc ttagccttac tagcatctga
    28441 aaacagttgg tcaagtgtaa aaggttgccc atcttctgtt aagtgaacct ttcctagact
    28501 ggtcttttca gtctcttcaa tcttaaaagc aactaaatca tagatttgtt tggtcacatt
    28561 acgattaacg actcccttta aacctgactc ttcttgctct gtatagtaga aaacaagatt
    28621 gtccttactt tctagcttgt ccttaacatc ttgatttatc agctccctaa cagaggaaat
    28681 cactttctcc ccttggagag gataataggc aatcacttcg gcttgcccct gacgaaaatg
    28741 ctccttctga tttccctcac tcaattgatc atctttctct tttttgagcg attcaatctt
    28801 ttgctcaaaa cttttttgct ggtagatctt aaaagcaata aatccgccta ataggcaaat
    28861 agaaattgct aaaataccaa tcaaagccaa taatacatgt ctcgttttcc cgtgtctgcc
    28921 acgtcctagt ctacttttat tcatatttat atcataacaa atttgagcaa taatttcaat
    28981 gatacaattt ggcaatccac aattcctcta gcaagccaaa gcatcttaca tcaactccaa
    29041 catccgtatc tgtagagatt atgtagtttt ctatctatgg aatttctagt agattgaagc
    29101 tagaatagta gacctatact tctaaaacat tgttagaaat cgatttgact gtcctgatcg
    29161 atttgtcctg ttctcatttc attttactat aaaactatga aaagctaggt catctgagaa
    29221 gtaattacta tcgacaattg cgatagcgta tactagtgcg atttgtttgt agctctggtg
    29281 ggtatcacct tcacgttggc gaattttttc taggttttga ttaacctgac tgcacagata
    29341 aggccatagg cgattgatga aaaaattctg atgatgaacc tgaatttcga tgataacttg
    29401 gattccatta tctaactccg ccaagacgtc tatactagta tagaaatctt acgctgagta
    29461 gggcatggaa ggcaagacgt gaatgttact tccctccaaa atcgtcacat tttttgctgg
    29521 caagtccagc atatcgcgga taaattgaca aatcattagt tgggctgata cctagatgtc
    29581 tgagaatcct ccttttcctc cttctattat agcacatttt taaatctagg tttgctagat
    29641 tctatgcttc tatctattta ttcggaaaag aaagctaata gcttatttga aattttctcc
    29701 tctctgttgg atagtcccgt ctatctatgt tataatgaaa gaaggattta aaatcggaaa
    29761 aggagtattt atgctaaaat taggtgtcat cggaactggc gctatcagcc atcatttcat
    29821 agaagcagcc cataccagtg gagaatacca actggtcgca atctattcta gaaaactaga
    29881 aactgcagca acctttgctt ctcgctatca gaatatccaa ctattcgatc aattagaagt
    29941 cttcttcaaa tcttcttttg atttagtcta tatcgccagt ccaaactcct tacattttgc
    30001 tcaggcaaaa gctgccttgt ctgctggtaa acatgttatt ctcgaaaagc cagctgtctc
    30061 tcaaccacaa gaatggtttg atttgattca aacagctgaa aaaaataact gttttatctt
    30121 tgaagcagct cgtaattacc acgagaaagc ctttactact atcaaaaact ttttagcaga
    30181 taagcaagtg ttaggagcag atttcaacta tgccaaatac tcttccaaga tgcctgactt
    30241 gttggctgga caaacaccaa atgtcttttc agaccgtttt gctggtggag ccctcatgga
    30301 tttggggatt tatcccctct atgctgctgt tcgtcttttt ggaaaagcca atgacgcgac
    30361 ttaccatgct caacagcttg acaatagcat tgacctaaat ggagatggta tcctcttcta
    30421 cccagactat caagttcaca tcaaggctgg aaaaaacatc acttccaatc ttccttgcga
    30481 gatttataca acagatggaa ccttgactct caacacgatt gagcatattc gctcagctat
    30541 ttttaccgac caccaaggaa atcaagtcca gcttcctatc caacaggctc ctcatacgat
    30601 gactgaggaa gtcgctgcat ttgcacacat gatccagcaa ccagatctga atctctacca
    30661 aacttggctg tatgatgcag gctctgttca tgagctacta tataccatgc gccagactgc
    30721 tggcattaga tttgaggcag aaaaatgaaa accaaactac cgactgaatg gcaagaactg
    30781 agtgaccaac tcggtttcca agaattcacc cccattcaaa ctcaactatt tgagcccctt
    30841 cttgctggag aaaacctcct aggagtgagc caaaccggaa ctggtaagac cctagcgtat
    30901 ctcctaccaa gtcttctcag actacaaaag aaaaaagccc aacaactctt gattctagca
    30961 ccaaatacgg aacttgctgg acagattttt gatgtatgta aaacgtgggc agaagctatc
    31021 ggattgactg ctcaactctt cctttcaggt tcaagtcaga aacgacaaat tgaacgtctt
    31081 aaaaaggggc cagaaattct gattggaact cctggccgta tctttgaact cattaaactt
    31141 aaaaaaatca agatgatgaa tgtggaaacc atcatcctgg atgaatttga ccaattacta
    31201 gatgattctc agattcactt tgtagaaaaa atcactcact acgcacctcg tgaccaccaa
    31261 ctggtctaca tgagtgccac gaccaagttt gaccaagaaa agattgtgcc gaatacacgc
    31321 actattgatc tctctgatca aaaattggac aatatccagc atttctacat gcaggtagac
    31381 caacgtcatc gagtggatat gctacgaaaa ctggctcatg tagaggattt ccgtggtcta
    31441 gtcttcttca atagcctgtc agaccttggg aatgccgagg aaaaactaca gtatcgtgat
    31501 atattggctg tttccctcgc tagtgatgtt aatgttaaat ttagaaaaat catcttagaa
    31561 aagtttaaag ataaccaact aaccctgctt cttgcaactg accttttggc tcgtggaatt
    31621 gatatcgata gcctagaatg tgtcgtaaac tttgatattc ctagagatag cgaaacctac
    31681 acacaccgtg ctggccgtac tggtcgtatg ggcaaggaag gttatgttat cactctcgtc
    31741 actcatccag aagaaatcaa aaaacttaaa aagtttgcaa gtatccgaga aattgtccta
    31801 aaaaaccaag aactctatat caaataaagc cccattgctc tatacacatc cagtgtatgg
    31861 agctttttta taagcaattc aattttatac tcatctgttt tcaaaaagca ttctagtcca
    31921 tctccgatta acgatggact ttatcacctc cttctccaat ccttgtatga catcttggag
    31981 ttgattcatg acatcttcca aagttcgaaa ggctttattc ttaaatccac gtttacgaat
    32041 ctctttccac acttgttcaa tgggttcatc tctggtgtgt atggaggaat aaaggtaaaa
    32101 ccaatattag tcggaatctt taaggtactt gatttatgcc atatagcatt gtccataacg
    32161 agtaaaagat aatcatctgg ataagcttgt gaaagctctt ctaaaaaggc gttcatccac
    32221 tcagtattac atccaccagc tattaagaaa aatgattcgc ctgtatgggc atcaacagct
    32281 ccataacaat agcgaaattc tcgtatatag tgactatgga catgtggacc tactcctatt
    32341 ggagaccaac aagatcccag tttactgatt ctaccgaaac cagcctcatc ttggtacatc
    32401 aagcgaacct tatgaaaagg tctactggtt ttaaagcgct ttcttgtgta ctgcccccaa
    32461 acagttagac aaataattta tccaaaggat ttagttctgt actgcacagg actaagtcct
    32521 tttagtttta tcttaatttt cttattgttg taataatcaa tatagtctat aatggcttgt
    32581 tccaattg
//
LOCUS       DAVHEX010000020        29434 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVHEX010000020 DAVHEX010000000
VERSION     DAVHEX010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29434
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.020"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..112
                     /locus_tag="VVL45_000890"
     CDS             <1..112
                     /locus_tag="VVL45_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEU4187085.1"
                     /translation="LEDVMNQLQDVIQGLEKEVIKSIVNRRWTRMLFESR"
     gene            complement(229..576)
                     /locus_tag="VVL45_000891"
     CDS             complement(229..576)
                     /locus_tag="VVL45_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU4187086.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYANDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(687..2462)
                     /gene="spxB"
                     /locus_tag="VVL45_000892"
     CDS             complement(687..2462)
                     /gene="spxB"
                     /locus_tag="VVL45_000892"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEU4187087.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAVSKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            complement(2669..4912)
                     /locus_tag="VVL45_000893"
     CDS             complement(2669..4912)
                     /locus_tag="VVL45_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU4187088.1"
                     /translation="MTEIVKASLENGIQKIRIRAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYTVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLTGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDVIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQSLHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKENAKLAISQLKKRGLRTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            complement(4922..5293)
                     /locus_tag="VVL45_000894"
     CDS             complement(4922..5293)
                     /locus_tag="VVL45_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEU4187089.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEITPE
                     QAGEFGFACGMNMMHGKMIVE"
     gene            complement(5304..5699)
                     /locus_tag="VVL45_000895"
     CDS             complement(5304..5699)
                     /locus_tag="VVL45_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEU4187090.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     ARLVDKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     regulatory      5883..5981
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6065..6856
                     /gene="thiD"
                     /locus_tag="VVL45_000896"
     CDS             6065..6856
                     /gene="thiD"
                     /locus_tag="VVL45_000896"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU4187091.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSNARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     gene            complement(7066..7698)
                     /locus_tag="VVL45_000897"
     CDS             complement(7066..7698)
                     /locus_tag="VVL45_000897"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU4187092.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARKVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPV
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLKKLHNILS"
     gene            complement(7691..8494)
                     /locus_tag="VVL45_000898"
     CDS             complement(7691..8494)
                     /locus_tag="VVL45_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEU4187093.1"
                     /translation="MQEFTNPFPIGSSSLIHCITNEISCEMLANGILALGCKPVMADD
                     SREVLDFTKQSQALFINLGHLSAEKEKAIRMAASYANQSSLPMVVDAVGVTTSSIRKS
                     LVKDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYPG
                     MSFLVTGPKDLVVSKNQVAVLGNGCTELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            complement(8494..9018)
                     /gene="thiW"
                     /locus_tag="VVL45_000899"
     CDS             complement(8494..9018)
                     /gene="thiW"
                     /locus_tag="VVL45_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEU4187094.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFHYSALGE
                     ILGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFSERID"
     gene            complement(9023..9715)
                     /gene="tenA"
                     /locus_tag="VVL45_000900"
     CDS             complement(9023..9715)
                     /gene="tenA"
                     /locus_tag="VVL45_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEU4187095.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEISDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            complement(9726..10376)
                     /locus_tag="VVL45_000901"
     CDS             complement(9726..10376)
                     /locus_tag="VVL45_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HEU4187096.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALTLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSVSLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            complement(10378..11763)
                     /locus_tag="VVL45_000902"
     CDS             complement(10378..11763)
                     /locus_tag="VVL45_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4187097.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQMI
                     AQVSSLPYSGQVLIDGSEVSQLSIVERVQTVGILLQNPNHQFTMESLFEELVFTMENI
                     GYHLQEIDSKIAEVVQQCRCKDILHRLIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELVYDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMNRVTGELGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGSITWKGSVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            complement(11764..12324)
                     /locus_tag="VVL45_000903"
     CDS             complement(11764..12324)
                     /locus_tag="VVL45_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU4187098.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGFVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     regulatory      complement(12586..12674)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(12883..13512)
                     /locus_tag="VVL45_000904"
     CDS             complement(12883..13512)
                     /locus_tag="VVL45_000904"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU4187099.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFGTQSKDDAGGTIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNMDGVSIISAISYAKNIEKTVREMSEQ"
     gene            complement(13514..14296)
                     /gene="thiM"
                     /locus_tag="VVL45_000905"
     CDS             complement(13514..14296)
                     /gene="thiM"
                     /locus_tag="VVL45_000905"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEU4187100.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVMIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLKTAVLVY
                     NIAGEIAEKRPNGHLPGTFKVEFINALYEITDEDVKEFKRVK"
     gene            complement(14533..15201)
                     /locus_tag="VVL45_000906"
     CDS             complement(14533..15201)
                     /locus_tag="VVL45_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HEU4187101.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     regulatory      complement(15416..15504)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(15758..16894)
                     /gene="lctO"
                     /locus_tag="VVL45_000907"
     CDS             complement(15758..16894)
                     /gene="lctO"
                     /locus_tag="VVL45_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEU4187102.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCNVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGTQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     gene            complement(17517..19007)
                     /gene="lysS"
                     /locus_tag="VVL45_000908"
     CDS             complement(17517..19007)
                     /gene="lysS"
                     /locus_tag="VVL45_000908"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEU4187103.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLITKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(19135..19281)
                     /locus_tag="VVL45_000909"
     CDS             complement(19135..19281)
                     /locus_tag="VVL45_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HEU4187104.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAGDTF
                     TSFQ"
     gene            19507..20175
                     /locus_tag="VVL45_000910"
     CDS             19507..20175
                     /locus_tag="VVL45_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU4187105.1"
                     /translation="MVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHYRILV
                     LAQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSYMAESFR
                     SGLEAISQTQQEIGLAIGLTPLQVFYYVVLPQATAVALPSFSANVIFLIKETSVFSAV
                     ALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRLRHAGFGNPST
                     LSRK"
     gene            20135..20812
                     /locus_tag="VVL45_000911"
     CDS             20135..20812
                     /locus_tag="VVL45_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU4187106.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIGISILSVLLSMMFRTVMG
                     IIMTSHSRIIRFLTRLYLEFIRIMPQLVLLFIVYFGLARNFNINISGETSAIIVFTLW
                     GTAEMGDLVRGAITSLPKHQFESGQALGLTNVQLYYHIIIPQVLRRLLPQAINLVTRM
                     IKTTSLVVLIGVVEVTKVGQQIIDSNRLTIPTASFWIYGTILILYFAVCYPISKLSTH
                     LEKHWRN"
     gene            20813..21571
                     /locus_tag="VVL45_000912"
     CDS             20813..21571
                     /locus_tag="VVL45_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU4187107.1"
                     /translation="MSETILEIKELKKSFGDNPILQGLSLEIKKGEVVVILGPSGCGK
                     STLLRCLNGLESIQGGDILLDGQSIVENKKDFHLVRQKIGMVFQSYELFPHLDVLQNL
                     ILGPIKAQGRYKKEVTEEALQLLERVGLLDKQHSFARQLSGGQKQRVAIVRALLMHPE
                     IILFDEVTASLDPEMVREVLELINDLAQEGRTMILVTHEMQFAQAITDRIIFLDQGKI
                     AEEGTAQAFFTNPQTKRAQEFLNVFDFSQFGSYL"
     gene            21583..22454
                     /locus_tag="VVL45_000913"
                     /pseudo
     CDS             21583..22454
                     /locus_tag="VVL45_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
     gene            complement(22541..23188)
                     /locus_tag="VVL45_000914"
     CDS             complement(22541..23188)
                     /locus_tag="VVL45_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4187108.1"
                     /translation="MNEIITLKNIELKLKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(23185..23883)
                     /locus_tag="VVL45_000915"
     CDS             complement(23185..23883)
                     /locus_tag="VVL45_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187109.1"
                     /translation="MGKLSSHMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLVLFCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFQ
                     SIFYFTRQKRRFIE"
     gene            complement(23893..24645)
                     /locus_tag="VVL45_000916"
     CDS             complement(23893..24645)
                     /locus_tag="VVL45_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187110.1"
                     /translation="MKQFVQFYKKDFLAVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTATLFLLIFLIAFSNGFSFSLEIKEVDFLREFYGISIANNASFFIGFFFSY
                     IAYYFFLSLLTISSFSWFKKSNMSLVFLFTFLFVESLFWIYQLDNGIIGLLPIFQYMV
                     NSNPYALIYWLTLLSIIIPLTVFSVHRNWRRV"
     gene            complement(24645..25409)
                     /locus_tag="VVL45_000917"
     CDS             complement(24645..25409)
                     /locus_tag="VVL45_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187111.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKKYLAIVG
                     STVIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQFLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKIGAIFIPVAYMMVGGIFWAILGLSYLEPVTTLALGY
                     QKDISLSLVSAHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            complement(25505..25908)
                     /locus_tag="VVL45_000918"
                     /pseudo
     CDS             complement(25505..25908)
                     /locus_tag="VVL45_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(26088..26720)
                     /locus_tag="VVL45_000919"
     CDS             complement(26088..26720)
                     /locus_tag="VVL45_000919"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEU4187112.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            complement(26754..27455)
                     /gene="pyrF"
                     /locus_tag="VVL45_000920"
     CDS             complement(26754..27455)
                     /gene="pyrF"
                     /locus_tag="VVL45_000920"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEU4187113.1"
                     /translation="MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKILSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQDWN"
     gene            27774..27866
                     /locus_tag="VVL45_000921"
                     /pseudo
     CDS             27774..27866
                     /locus_tag="VVL45_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516271.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(28201..28914)
                     /locus_tag="VVL45_000922"
     CDS             complement(28201..28914)
                     /locus_tag="VVL45_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4187114.1"
                     /translation="MLKRFLALVWLRCQIILSNKSILLQVLVPFAFTYFYKYLMETQG
                     KVNDQQALVLLMMCLPFSFSLAVGSPITIILSEEKEKYNLQTLLLSGVKGSEYILSTM
                     FLPFLLTFVIMGTTPLILGVTIVHTFNYITIVLLTSLSIILFYLLIGLTAKSQVVAQV
                     ISLPAMILVAFLPMLSGLDKTVAKITDYSFMGLFTKFFTKWEEFSWNKTLIPNLTLLI
                     WIVLLLTLITITIRKKKIS"
     gene            complement(28907..>29434)
                     /locus_tag="VVL45_000923"
     CDS             complement(28907..>29434)
                     /locus_tag="VVL45_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4187115.1"
                     /translation="NISKSRVDNLLKRVGLYDSRKMVAGKLSTGMRQRMLLARALINN
                     PAVLFLDEPTSGLDPTTSRTIHELILELKTAGTTIFLTTHDMNEATLLCDYVALLNKG
                     KLVEQGAPSELIQRYNKDKKIKVTDYNGNQITFDFTSLEQVSQTDLENIFSIHSCEPT
                     LEDIFITLTGGKLNA"
BASE COUNT         9116 a         6303 c         5257 g         8758 t
ORIGIN      
        1 tttggaagat gtcatgaatc aactccaaga tgtcatacaa ggattggaga aggaggtgat
       61 aaagtccatc gttaatcgga gatggactag aatgcttttt gaaagcagat gagtattata
      121 tgcaatttct ttatataaaa agaccggatt gctccgatct ttcaatagtt catattctca
      181 atttctattt taaaaatagc taaggttaac gtcaaatgac tacgcgacct atttcatacg
      241 ataaaaatca agcactagac cagcaggtcc ttgaactaat aaggactctg ttccccaatc
      301 ggttacagtt ggtccgtgta aaacctttat accaagctcg ttcaaccgtt tgtagttctg
      361 gtctacatcc tcaacctcga tatgaataat gattcctgac tgaaagtttt ccaaaggaac
      421 caaatgattt tgtgacaaca taaggcagtg actaccaatc gtaaactgag caaaaccatc
      481 attagcataa tctgcctttt tatccaagat atgctccaag tcagcacaga cttggggaac
      541 atttgaaacg ataatatcta attgatttaa attcatttac tctcctccat aaaaagaccg
      601 gattgctccg atcttttaaa gttctgctct atgaaaatca aagaataaag tctacaagtt
      661 tcatatttga ttttcggcga gaggaattat ttaattgcgc gtgattgcaa tccttcttct
      721 tccaagaaga gacggaatgg tacgagttct tctgcttcgt atttttcctt gaaggctttg
      781 atagcttctt ctgagtgaag ttttggatcc aattcaagta cttctactgg aagtggacgg
      841 tgttgagtga tgcgagcatc gatgacaaca gttttacctt ctttgttcaa tttaacagct
      901 tctgcaacaa ctgcatcgat gtcttcgata cggtcaactg tgaatccaac agctccttga
      961 gcttccgcaa ttttagcgta gtcagcgttt gtgaagtcta caccaaacaa gtgtttgttt
     1021 gtatcttcgt atttgttctt gatgaagccg tactcagcat ttgagaagac aaggttgata
     1081 actggaaggt cgtattgaac gtttgtgata acgtctgggt agcacatgtt gaatgctccg
     1141 tcacccatga tgttccatac ttggcgatct ggattgtctt tcttagcagc gataccacca
     1201 ggaagggcaa tacccattgt cgcaaagagt ggagatgtac gccacatgtt cttaggtgtc
     1261 atgtgaaggt gacgagtaga tgtttgagta gtgttaccta cgtcgattga gtagatagcg
     1321 tcttgatcag catgtttgtt gattgcattg taaacttgat acaattgcaa ttcaccctca
     1381 gttttacctt cgagtttgtt catgtaatca cgccagtttt ggttgttctt aacgtttgca
     1441 cgccaccatg gagttgattc aactgggttt actttgtcaa ggatagcttt agctgcttga
     1501 ccagcatcac caaggattga agcgtcaagg gcatgacgtt taccaagttt gtaagggtcg
     1561 atatcgactt ggatgaattt ttcagtgttc ttgaatgctt cgtaaacttc agcaaatggg
     1621 aagtttgaac caaggaaaag aactgtgtct gcttcaaaga ccacttcgtt ggctggtttc
     1681 caaccaacac ggtaagcaga acctgtcaaa ccttcatagt tccattcgaa agcttcaaag
     1741 tttttaccag ttgtgatgat tggtgctttg attttacgtg acaattcagt aatcacttca
     1801 ccagctttaa caccaccaaa tccagcatag ataactgggc gttcagcatt gttcaagatt
     1861 tcaacagctt tgtcgatttc aacttcgttc aaagcaggag cgatgaatga gcgttcgtat
     1921 gaacctgaac cgtagtatga gttttcatcg atttcttgga aaccgaagtt tactggaatt
     1981 tcaacaacag ctggaccttt tttagaaact gcagcacggc aggcttcgtc aattactttt
     2041 ggcaattgct cagcgtaagc tacacgtttg ttgtaaacag cgataccgtt gtacattggg
     2101 ttttggttaa gctcttggaa agcatccatg ttcaattcgt taactggacg tgatccaagg
     2161 atcgctagga atggagtgtt atccatagct gcatcgtaaa caccgttaat caagtgagtc
     2221 gcacctggac cacctgaacc aactgcaacc ccgattgagc cgccgaattt agcttgcata
     2281 accgctgcaa gagcacctgt ctcttcgtgg cgaacttgta agaaacggat atctttgtct
     2341 tcagccaaag cgtccatcaa tgagctgagt gttcctgatg ggataccgta gattgtatct
     2401 acgccccatg ttttcaatac gttaagcatt gctgcagatg cagtaatttt cccttgagtc
     2461 ataatgataa ctctccttca atttttttaa acttggagaa tacgattaca tagaattgga
     2521 aacgttctcc aaatttttac tattccactg tatcatattt atgctgactt ttctaaaaat
     2581 ctgctcaaaa ctctctattc tctattctaa tacagttttg aaagttctgt catttctgtt
     2641 ttataacaaa gaaatctagt cattactttt agtctatttt actaaaattt aacagaaggg
     2701 aactggtcag aacagataca gaactaaagg ccatggctag acctgccaat tctgggttga
     2761 gagccagtcc aacacctgaa aagactcctg ctgcaatcgg aattccgaca acattgtaga
     2821 taaaagccca gaaaagattg agtagaattc gatgaaaggt tttcttactc atatcaaagg
     2881 cacgaaccac tcctaaaaga ttattggttg tcaacaccaa atctgctgac tcgatggcga
     2941 tatctgttcc agctcccata gcaatcccca catctgctac actaagggca ggagcgtcat
     3001 tgataccgtc cccaacaaag gctactttcc ctgactgttg cagtttatgg atttcatggg
     3061 ctttttcttc tggcaagacg cctgcaatga cctcttcaat tccgatttga tctgcaatag
     3121 cacgcgccac accagcattg tctcctgtca gcatgactgt tcggagacca cgttttttta
     3181 gctgactgat ggctagctta gcattttcct taggaatatc ttgcaaagca agcaagcctt
     3241 tgatttcatt gtcaacagct aagaacacaa ctgtcttagc ttctttttct agttcttcta
     3301 gtttatcttg ataagtatta gaaatatcca tgccatccag cattttagca tttccaagta
     3361 aaacttgttt tccattgatt cgccctgaaa cacctttccc gtgcaaggac tgaaaatttt
     3421 caacagtttg aaactcaagt ccagcttcac tcgctcgctt aacgatagcc tcagccagtg
     3481 ggtgttgaga agcatcttcc aaggaggctg ccaacccaaa cacttctact tcgtcgccga
     3541 tgacatctgt taccacaggt ttcccttccg tcaaagtccc ggtcttatca aagacaaggg
     3601 tttgaacttt ctggatttcc tgtaagacag ttccattttt gaggagaacc cccatcttgg
     3661 cactacgtcc tgtccccacc ataagggctg tcggtgttgc aagtcccaag gcacaaggac
     3721 aggcgataat caaaaccgcc actccgtaga gaagagagga cacaaagcta gctccaagca
     3781 caaccacact atccctgagc aagacgaacc aaacccaaaa ggtcatgatt cctaaaatga
     3841 caactactgg gacaaaaatc cctgaaatct tatccgtcaa gtcctgaatc ggcgcacgac
     3901 ttgtctgagc tttcttcaca aaatccacaa tctgagccaa aacagtctct gagccaactt
     3961 tttctgctct aaagacaagc gttccactat gattgatggt tgagccaatg acagtatctc
     4021 caactgtctt gtccacaggc agactctcac ctgtcaccat ggattcgtca atactagaga
     4081 caccttctac tacgacacca tcaacagcaa tcttttcacc gggacgcact cgaatcaggt
     4141 cgcctacctt gacttgttcc aaaggaactt ggacataact atcatcactc aagacttctg
     4201 cggttttagc ttgcaagtcc agtaatttct ccacagcttg ggacgtattt tttctcattt
     4261 tttcctcaaa aactgctccc aaaagaacga aaaagaggat aaatccagca ctttcgaagt
     4321 aaacagggag accagcaaag agagcaacta ggctatagaa ataagccact agagttccca
     4381 gcgcaaccaa ggtatccatg ttggcattgt gctttttaaa actggcccaa gcactctgga
     4441 tatatggctt acctgcaact aacataatag gcgttgttgc tagaaaggtt ccccaatgca
     4501 tgacttgatg actaatgcta cctgtcaaca tcccaatcat gagaatcaca agaggcacag
     4561 taaagatact agtaatccaa aaacgttgca ggagagatag agattttcga gtcttctcaa
     4621 cgactgtata gcttcccttt tgcatcttca tgccacaaga aaattcatgt cgccctaatt
     4681 cttgaggcgt aaaacgaatg actttctcct catctacgcc gattggttcc aagatacctt
     4741 cttcttcaaa cagaatttcc ttataacagt ttgaaggagt agcacgatga aaggtaatct
     4801 cagctggaat tcccttttga agctggatat gggctggatg atagcctttt tcagctcgga
     4861 tacggatttt ttgaatgcca ttttctaagc ttgctttcac aatttctgtc atagtctcca
     4921 cctactctac aatcatcttg ccgtgcatca tgttcatacc acaagcaaag ccaaactctc
     4981 cagcctgttc aggcgtgatt tccactacat actcttcccc cattggcagg ttcgcatgta
     5041 caccaaaatc tggaaaaaca atttgatcca gacatggtga aggatccttg cggtcaaaga
     5101 caatgcgtgc tggcactgat ttcttgagga caatcaactc aggagtatag cctcccatga
     5161 cttccactcg aatctcttgg tatccgtttt tttgctgggc tttttgtcca gatttttcag
     5221 gctttttgaa aaaccaaaac aagataaacg cgataagggc aatacaaata atggttacaa
     5281 tactatttaa catgacgtct cctttacata caattacatc ttacttctgt tacagcactt
     5341 gatttcttct ctgaaatcac agcttccaag tcttccaagt cagtctgagt aaattcacat
     5401 tctacaatca agtcagccaa caaattccta atcctacggg aacaaacctt gtctttgata
     5461 tcttggacaa gtaaatcccg actttggtct agagttaaaa gggctgaata aacaaaggac
     5521 ttgccttctt ttttccgagt caaacactct ttatcaacca gacgagccaa aagtgtctga
     5581 accgtggact tggaccagtc aaaccgctct gccaaaaccc taatcaaatc tgtactggtc
     5641 tgctccccct gcatccaaat aatcttcatg acctgccatt ctgcatctga aatctgcatt
     5701 accatacctc caaaatctac atttgtcaat tacactcatc agtatactct taaaatctac
     5761 atttgtcaat tatagaaata atattttctt cgaaaaatag aattttaatc atttgaaaaa
     5821 cgatttgcag tcaaatatta ctatataaac aataaaaata tgctatacta aagaaaaaag
     5881 aaaacaacca ctaggggtgc gtaaagctga gattaacgac tgttagatcc ctctgactca
     5941 atctaggtaa tgctagctga tggaagtgga aatgataatg gggactagca gtcttctatt
     6001 gcctttctaa aacagactag cttgttctta agaatacaaa cttcagttgg ttgggaggtt
     6061 ttagatgact tatttacccg ttgctttgac cattgcaggg actgacccta gtggtggtgc
     6121 tggcattatg gcagatttaa agtcattcca agcgagagat gtctatggaa tggctgttgt
     6181 aaccagtctt gtcgctcaaa ataccagagg tgttcagcta atcgagcacg tttctcctca
     6241 aatgttgaaa gcccaattgg agagtgtctt ttctgatatt ccacctcagg ctgtaaaaac
     6301 tggaatgttg gctactactg aaatcatgga aatcatccaa ccctatctta aaaaactgga
     6361 ttgtccctat gtccttgatc ctgttatggt tgctacaagt ggagatgcct tgattgactc
     6421 aaatgctaga gactatctca aaacaaactt actacctcta gcaactatta ttacgccaaa
     6481 tcttcctgaa gcagaagaga ttgttggttt ttcaatccat gaccccgaag acatgcagcg
     6541 tgctggtcgc ctgattttaa aagaatttgg tcctcagtct gtggttatca aaggcggaca
     6601 tctcaaaggt ggtgctaaag atttcctctt taccaagaat gaacaatttg tctgggaaag
     6661 cccacgaatt caaacctgtc acacccatgg tactggatgt acctttgctg cagtgattac
     6721 tgctgaacta gccaagggca agagtcttta ccaggcagtt gataaggcca aggcctttat
     6781 cacaaaagct attcaagatg cccctcaact cggtcatggt tctggtccag tcaaccatac
     6841 aacttttaaa gattaagaaa aaaaactctc tagttcccac tttaagggaa ttagagagtt
     6901 tttatactct tcgaaaatct cttcaaacta cgtcagcttc catctgcagc ctcaaaacac
     6961 tgttttgagc tgacttcgtc agtcttatct aaaacctcaa ggcagtactt tgagcaacct
     7021 gcgactagct ttctagttta ctctttgatt ttcattgagt attaattagg aaagaatgtt
     7081 atgcaacttt tttaaaaagg cttgcgtttt tgcctcaata tcttctgctt gcatcaaatc
     7141 acgtacaaca gctacaccag ctatgccagt gcccataagc tgatcaatat tctccgaagt
     7201 caagcctcca atagcaacta ctggaatggc aaccgtttgg caaattgttt tcaaggtcga
     7261 tatcagagta atgggcgcat tttccttggt ggtggttggg aaaatggctc ctgtacccaa
     7321 gtaatctgca cctgatttct ccgcttccag agctctttta accgttttag cggtgacacc
     7381 gaggattttt tcaggaccca agactttgcg agctaccgaa actggtaatt catcatctcc
     7441 gatatgcaga cctgctgcat caaccgcaag acaaacatcc aaccgatcat cgattatcaa
     7501 gggtacctga taagcatctg ttatttcctt gacttgtttt gccagttgat aatattgatt
     7561 ggttgtgaga tttttttctc gcaattggac tatggtaacc cctgaacggc aggccgtctc
     7621 aacttttgca agaaagcttt ccacggaatc ttgatagcga ttggttacca gatatagtct
     7681 aagtgcttct ctattcataa acctctcctt tgatggtatc tagccaattt tcatctcttc
     7741 ttaggagcga aagctgattg agtacttggt aacgaaattc ttccaatccc attccttgaa
     7801 caactatttt ctcagcagcg atattgagat aagagactgc taagcaagaa gcttcaaaac
     7861 cagtctttcc ttggctgaga aaaacagctg ttaaggctcc aaccaagtct cctgtccctg
     7921 ttatccagtc taattcagta cagccatttc ccagtacagc gacctgattt ttcgaaacga
     7981 cgaggtcctt gggacctgtg actaagaaag acataccagg ataggtctga caccagtctt
     8041 tcaagacttg aagcaaatcc tccgtttctt gatctttagc actcgcatcg accccaacgc
     8101 cgtggtgctt taatccaaca agacttcgaa tttctgacat gtttccttta aggaccgtag
     8161 gtctatagtc taaaaggtct ttaactaagc tcttacgaat ggatgaagtc gttacgccaa
     8221 ccgcatctac taccatcggg agagaagatt ggtttgcata cgaagctgcc atgcggattg
     8281 ctttttcctt ctcagctgac aaatgcccca aattgatgaa gagagcctga ctttgcttag
     8341 taaaatcaag aacttcacgg gaatcatctg ccatgacagg tttgcatccc agagccaaaa
     8401 tcccatttgc cagcatctca caagaaatct cattggtaat gcagtgaatg agggaactag
     8461 agcctatagg aaagggattt gtaaattcct gcatcagtct atcctttcac taaagaaata
     8521 tccctgcact tttttaaaga attcctgctt gattaaaaat cgaaaggcaa taaaggaaat
     8581 cgctgtacca atcaaggttg ctccgaaaaa tcgaggcgtg tagataaacc agctaagctt
     8641 agcagctgat cctgtaaaga gtaccataac aggataggaa acaatggaac caataatacc
     8701 tgttcccaaa atctctccta gagcagaata gtgaaatttt cgaccgtact tataaaagag
     8761 acctgctaga agggctccaa aagtcgctcc tgtgagagct aaaggcggaa tcccttgagt
     8821 cgtcatacgg ataaaggctg tgactgtagc catagccaag gcataaacag gtcccatcat
     8881 gattcctgct agaatattga ctacactgga catcggtgcc attccctcaa ttcgaaagat
     8941 aggtgtaagg actacatcaa gggcaatcat catagataaa atggttaatt tgtgaacttg
     9001 taattggtgc tttctcatgc ttctattctt ctcctttttc taaagactgt aaatcgctct
     9061 tccatgtctg gtgttggtag gccatttccc aaaacttggc ttccatatga acactgatgt
     9121 ggaaggcatc tagcattttt tgcttgtctg tctcgtcact ttctcgatag agctgattga
     9181 ccagtgctcc ctcctctctg atctgttgct ctaactcatc cgtaatataa gtttcaatcc
     9241 attgttgata gagaggattt ggtgatggtt taagattaag tgatttgcct atatcatggt
     9301 ataaccaagg acaaggaagc aagcttgcaa aagcgatggc taagttcggt tctgcaaatt
     9361 gcctataaat atgagaaatg taatgataac aggttggagc gattggatgt tgctccattt
     9421 cctggtcgct gatttccaat tccttgaaaa attgttggcg aataaataac tcaccctcca
     9481 ctaaaccctg agcattttgt ttcaagagtc ttttcatctc ttggtttgaa gtcttatcag
     9541 ccaaaagatg atagatttct gagaaagcct tcagatagta ggcatcctga atcaggtaat
     9601 agcggaaaat ggcaggttct aaattcccct cttgtaattg taaaataaag ggatgatgaa
     9661 aggaagcctg ccaagctttc ttggataatt ccatcgcaat atctgtaaat tccataataa
     9721 ctcctttata aaaatagact ggtttgaagc aataaaaaga aaagcaggta gattaatttt
     9781 gtttttttag gaatataaaa agtccgatag ctattcttca actgtgcatg ttcgtcatat
     9841 ccgtgagcag atagagctct caggtaaaga tggcgccacc taaagactgt catcagaacc
     9901 ttactgtaaa tcaagggcga ccaaaaatgt agttcttgac cacgtaatag gcaagcttct
     9961 ttgagggact tgatttcttg ctgaatgaga ggaaaagaat tgaataccac aatcaaggca
    10021 taggaccaag agcgtgatag ccccttttga gccaagtaca agagaagctc ttttagtgaa
    10081 acagaggaaa caaagacaag gccgatacaa actgtcacaa aggccctcgt tccaagcatg
    10141 actgcctgtg aagcatctcc gtgtaactga actgcccagt agttggcaaa agatggtaaa
    10201 atggcaagta tgatcatcca agctaacatt ttaaatcgac ggtaatagag cataaagaga
    10261 atacaaaatg cgactaccga aagagtcaga gcaatcgaag gaatgaaaga tgtttccaag
    10321 gataaaatca gcaagaagag actgataatc ggtgtctggg ttgctacttt gaccatacta
    10381 tctcacctcc ccttgggtat tgctactctg agatgtaagt ggtttggtaa tggtcacttc
    10441 tttcacatgc cgaagaccct gactagtcat ctcaatccaa taatcaacca cagaaatcaa
    10501 agggtctaaa cgatgactaa tgagcagaaa acttcttcct tgattcctct cctccacaat
    10561 ccacttgcaa aaataatggc aggctctatc atccaaacct gcaaaaggtt catctagcaa
    10621 gatcacggaa gccttactgg tcaagatggt caggagctga agaatttttt gctgaccacc
    10681 acttaattga tagggactct tatcgactgc ctgctccaaa tcaaaatatc gtaaagcttg
    10741 aaaaatccgc tgatttcttt cagaatcagg tccatctaat tgaagctcct ctcgcagact
    10801 gactcggata aactgcttct cagcttcctg aacaacacca gtcagatcac gatacaaact
    10861 ctttttcttt ttcaggaccg aacccttcca agtaatgctc cccttatact tttgaaattg
    10921 aagaatagac cgaaagaggg ttgatttccc gacaccattg tcacccagga tacaggaaat
    10981 cccttgatag aatgtgaaat cagcaattga aaagaggggg cgattaccaa gctcaccagt
    11041 cacacggttc atatggaata gttccgggct agaagcaact tcctttgaag caacctgtgt
    11101 catctcatag gaagggattt gaaacacttc ccttagtttt ccgtctctta gctccaccat
    11161 atggtcgata taggctttat agtcagataa atcatggtcg cacaaaataa ctgtcttccc
    11221 atcatagacc aactctttta gaatctccaa tatctcgatt ctgctcttgc ggtcaatgga
    11281 agcgaagggc tcatccaaga gatagaccct aggattcatg gcaaagagga cagccagcgc
    11341 tgctttttgc ttttccccac ctgataagtg atggatgaga cggtgcaaga tgtccttgca
    11401 acgacattgc tggacaacct ctgctatttt agaatcaatt tcctgaaggt gatagccgat
    11461 attttccatg gtaaaaacca actcctcaaa caagctctcc atggtaaatt gatgattagg
    11521 attttgcaag agaataccaa ccgtctggac acgttcgacg atagaaagct gactgacctc
    11581 gctcccatct atcaggactt gaccgctata gggaagagaa ctaacttggg caatcatttg
    11641 aaagaggctg gattttccag acccactact cccaactaac aaggtaaagg cttgcgcatg
    11701 aaaagtaaaa tcaaacggct cagagaagat tggggactga atcgctcgta gttccagacc
    11761 catctatgct tttcctccag ttgcaaactg atgatagagt ttgacaatgg cacgaaccaa
    11821 gatggtacag aagaaataaa cagaaataaa acgtaccaca agcaaggaaa ggacaaacgg
    11881 aagggaaaag gcgtagtaac ctaacttaat gtattcatag acaaagctaa caagcgtaat
    11941 cccaatacta ttagcagtta gagagagcca actttcatag cgattcttag ttacgataaa
    12001 accaaattca cttcccaaac cttgaacaaa gccagacaaa agagctccta gaccaaattg
    12061 gctaccataa aggacttcag caagcgcagc tagcacttct ccaatcgttg cacttccgac
    12121 tctcggaaca aagatggcag caatgggcgc agccatacac cagagaccga agaggatttc
    12181 attggcaaag gcctgcaaac caagaggtgt taagagtaga ctgagaatat tatacacata
    12241 tcctgaacca acgaaaaccc caccaaaaaa gatagacaag aaagcaagca agataacatc
    12301 ttttaactgc cattttttca acataaaaaa ctcctttttt taaagaaaag tgaggcactc
    12361 aagaagaccg acctaaatac tttgtatagc agactgaatt tagaacagta cacaagaaca
    12421 ctaaaatatt tctagaaatt aatttgaatt ttctaattga tttgttcgca tcttatttca
    12481 atctactata tcatcttcat ccagtttcgt aaaagaaaaa actctaatta cagatacaaa
    12541 ttagagttca gcttacaaga ttagacagtt cttttcgaca tacgaaaaaa acatttcaca
    12601 tttcccttcg ccagtcttaa ctgtatcagg ttcaatgggt atcatctcag cctaaagcac
    12661 cccaaatgtc tttattattt aattatgtga ttattataac acacatttta tactagttca
    12721 agaaattgaa ctggaaatac agccttgcac tcacaaagac agcagatctt tcttttgcaa
    12781 aaaacaaatg acctgtttga tgaattagcc attcaagctg aatctggaca tagcttttta
    12841 aaaaaggaaa atcctactta cttagaatcc aaggatagat atctattgtt cactcatttc
    12901 ccgaacagtt ttttctatat tttttgcata cgatattgcc gaaatgattg aaacgccatc
    12961 catattggtc tttataatgt ctttaatatg tttcgtctgt atcccaccaa ttgcaactaa
    13021 aggcatttgt ggcaatagtt ttctcatcaa ttcaagacct tcataaccta tagtaccacc
    13081 agcatcatcc tttgactggg taccaaatac aggcccaaca cctacataat ctacatattc
    13141 aacttttgat tgttgaaatt cttcttcgtt tcttatagaa agaccaatta ttttatctgg
    13201 catcaatttt ctaatttcat caacaccaat atcatcttga cctacatgta cgccatcggc
    13261 gtcaatttcc attgctaaat ctatatcgtc attaacgata aatggaacat tgtatttttt
    13321 acaaagttct ttaatttgga tagctagctc aagtttttct aagccttcta aagcaccctc
    13381 acctttttct cgaaattgaa ataaggttat accacctttt aaggcttcct caacgactgt
    13441 atatagattt tttccttggc aagtagtcgt tccacaaata aaatatagtt ttagtaattc
    13501 tttatgaaac atcttacttc actcttttga attcctttac atcttcatct gtaatctcgt
    13561 ataaggcatt tataaattca actttaaatg tcccaggaag atgtccattt ggacgttttt
    13621 ctgctatttc tccagcgata ttgtaaacca acactgctgt ttttaatgat ttcaattctt
    13681 gacctttttc tagtccgata aagcttgcta ctacagctcc taataagcat cctgtcccaa
    13741 tgactttcgg catcatagca ctaccattat gaatcattac cacttctcca ttaacagcaa
    13801 tggcatccac ttcacctgtt actactattg gaatattgaa cttctcattt gctgctagag
    13861 caatttcgtc aatattatct acgcccgcac tatctactcc tttagatgcc acatctattc
    13921 ctactaaaga ggcaatctcg ccagcatttc ctctaatcgc tgctagttta taattgttga
    13981 ttagatcatc tgctactttt tttctatatt ctcctgctcc acaggctaca ggatctaaaa
    14041 ctgctgggac attatatttc tctgcaattt tcagagcagc ttggtataat ttccaatttt
    14101 catctgtcaa tgttcctatg tttattaata aaccaccagc atactttaac aaatcctcta
    14161 aatctgctgg aaactcactc atggctggtg aggcgcccag tgctactaat ccatttgctg
    14221 tgaaattttt tactacatca ttggttatac aaatgaccaa tggtgctttt tcttttaata
    14281 attttaaact tgtcatattg aaatccttcc ttttcacttt atacgatcta ctaatttcga
    14341 tttatcttta gttgagaatt tttttcattt acattgaatg atttatactc aatgaaaatc
    14401 aaagagcaaa ctaggaggct aaccgcaggt tgctcaaaac actgttttga ggttgtggat
    14461 agaactgacg tggtttgaag agattttcga agagtcttac ctcatcaaat ttgtaaatat
    14521 catgagcctt ctctagacat cgtaaccaat atcaaaaaaa gctaattcta aagcgactgc
    14581 ttgattccag cgttgctgaa gttctgtcaa atcttctcga tttttaccga cacgattgag
    14641 ttcgtcaacc agaaattgaa cccactctgc aaagaaagga cctctgtgga gattgatcca
    14701 ttccgaatga atatagactt caggtaaagc caaatcttta gaaccccagt ctaaatagag
    14761 accttctgca atgaccagca tgaccaaaag atgggcatag tctgatgaag ccaccgccga
    14821 atacattaga tcctgaaagg cttttgttac agggtgcaaa gtcacttcta gatagtcatt
    14881 ctctgctact tttaactctt taaaagcctt ttggaaataa ccatcttcat ctgcttcaag
    14941 aaagcctagt tgcttggcaa aacgaagctt ggattcaagt ttatctgcgt gagctacgca
    15001 ggcacccagc atggataaga aggcatcaaa gaagtgataa tcttgaatca gatagtcctt
    15061 taagacctta ttctcaattg tccccgcaaa aagttcctta acaaaacgat gattgattgc
    15121 agcctgccaa tccttctgac tgctttttaa taattctcca acagtcaaac ctggctgaaa
    15181 tgcatagtct tgtgtttcca tatttacttc tcctctcttt acttgttagt aattaataaa
    15241 acaccaagaa atatcaagca aaatcgtaat tccacttgat ccttttaaag cacatcgaga
    15301 gcatttgcag agagctaact aaacaagcct atccagttta tataaacaaa aaactccaat
    15361 tacaatcaag aattagagtt gacttacaag attagaccgt tcatttcacc atacgaaaaa
    15421 actgttcaca tttccctttg ccagtcttaa ctgtatcagg ttcaatgggt attatctcag
    15481 cctaaagcac cccaaatgtc tctattattt aactactgaa ccagtatagc aaaaaatgaa
    15541 agccctagca agatatttga ccgaaaaata tctttatata taatatattg aaactagaat
    15601 agtacacctc tacttataaa acattgttag aaatcgattt gactgtcctg attgatttgt
    15661 cctattctta tttcatttta ctatagtttt cgatagcaat ttattcttcc aatacacgaa
    15721 gaaaaacctc cacattcagt ggaggcaatc tgttttatca atacaatttt aagtcacgag
    15781 ggtcaactgg gaaggttggg ttgtatggat tgtgacggag cttgaagtgt ttgacatctt
    15841 caatggtctg agttccagac aattgcataa ctgtcttcaa ttccgcattc aagtgttcaa
    15901 agacttgacg cacaccgaca ctaccaccga gagccaagcc atagatgaca gggcgtccaa
    15961 tagcaaccaa gtctgctcct gatgccaagg ctttaaagac gtgttgacca cgacgaacac
    16021 cagagtcaaa gacaatcggc acacgtctat caactgcttc tgccacttct tgaagcgagt
    16081 caaaggcagc tggtccaccg tcgatttgac gaccaccgtg gttggttacc cagataccag
    16141 aagctcctgc agcaagcgaa cgttcaacgt cctcacggca ttgtggtccc ttgacataca
    16201 caggaagacc agagtattca gcgataaatt ctacatcgcg tggagacaag cgttgtttag
    16261 ctgatttgta aacaaagtcc attgatttac cagcaccttc tggcaggtat tcttcaacaa
    16321 tcggcatgcc aactgggaag acaaaaccat tacgcttatc cacttcacga ttccccccta
    16381 cagtagcatc tgccgtcaag acaatcgctt tataaccttc agccttcaca cggtccatga
    16441 tgtggcggtt gataccgtca tccttactaa agtaaaattg aaaccaatga ggtgtccctt
    16501 ggagggcttc agaaatctct ggaaggtcaa cagtagagta agaactggtt gtataaagag
    16561 aaccaaactc atgcacacca cgcgcagtcg ccacttcccc ctgttcattt gccaatttat
    16621 gagccgcaac aggtgccata atgattggag aagatagttt ttcacctgca aattcaatct
    16681 ctgtacttgg attttctaca ttgcaaagtg tatgaggaac gatgagcttg tggttaaagg
    16741 cacggatatt ctctcttaaa gtgaaagtat cttccgcccc actagcgata tagccaaatg
    16801 ctgctttagg aataacttgt tgcgccattg gctccaaatc ataggtattg atgaagtcta
    16861 catgaccttc tgcattgctt gttttgtatg acataaactg tcctcctcga ttaagtaagc
    16921 gtttgctttt tactatacaa ctataataac tcttttttca atcattttca agagttttgc
    16981 ttaaaaacca tcttattttt tcatacaata tactagccct actttcctta aactctaaac
    17041 cttagtctat attaagtgaa tcgttgagat tttttgctgc caggcatttg aactactttt
    17101 tatttgctga ttctatctac taatactata aaaaacaagt gaataagaca ccagaacatc
    17161 ctctaatagg acctatggac ttatatcaat cccatatata ctgtttaagc aagatactat
    17221 cctagttgac gctaacttcc tatcccactt attaattttt tatttatagt gaatctgaac
    17281 aaatcaattg gggaatccaa gtcaatttct agtacttttt gaaaagtttc agcgtactat
    17341 cgtaaaatga aataaaacat gcacaaattg cttagggaat ttaaaccaat ttctaacaat
    17401 gtcttagaaa tcaaagtgta ctatttcaat ttcaatatac tacacttcat tttctcctta
    17461 aattcagtct ctttttgaat caaaaaatcc tctgataaga cccagaggat aagaatttat
    17521 ttcattgttg ggaagagcaa tacatcacgg atagttgttg tatcagtgag gagcatgcag
    17581 agacggtcga taccgattcc caaaccacct gttggtggca taccgtattc aagagcttca
    17641 atgtagtcat agtcgattcc tgtcgcttca tcatcaccaa gttctttggc tttagcttgg
    17701 gcttcaaaac ggctaagttg gtcgattggg tcgttcaact cagtaaaggc attaccgtac
    17761 tccttagtca tgataaagag ctcgaaacgg tcagtaaagc gttggtcttc aggatttttc
    17821 ttagcgagtg gagatacagc tactggatgt ccatagacaa aggttggttg gattaaagtt
    17881 tcttcaacaa actcttcaaa gaaggcattg atgatgtgac caacctcagt gtagtgtttc
    17941 tcaactggaa ctttcttctc agcagcgata gctttagctt cttccaaagt catgtcttgc
    18001 cagaaatcga caccagtaat ttctctgata gcatccacca tatgaacacg cttaaatggt
    18061 tcgttaatct tgatttcagt accttggtag ttgactgggc catcaccttt gactgattta
    18121 gcagcgtgtt ggataatgcc ttcagtcaag tccatgatgt cttggaagtc tgcataagct
    18181 tggtaaactt cgatagaagt gaactcaggg ttatgagtag cgtccattcc ttcgttacgg
    18241 aagatacggc caatttcata gacacgttcc ataccaccca cgataaggcg ttttaagtga
    18301 agctcagtcg cgatacgaag caccatgtca atgttttggg cattgtggtg ggtgataaat
    18361 ggacgggcag cagcaccacc ggcttcatta tgaagaacag gtgtttccac ttcaaggaat
    18421 cctttttggt caaggtaacg acggatttca gagatgattt ttgaacgagt gacaaagcgt
    18481 tcaaagcttt cacgattaga aatcaagtca aggtaacgtt tacggtaaat tgtttcaacg
    18541 tctgtcaaac catggaattt ctcaggaaga ggacgaagag ccttagacaa gtgtgtgatg
    18601 tgggttgcct tgatagagag ttctcccata tccgtacgca tcacttcacc ttcgacacca
    18661 aggaagtcac caaggtctgc ttttttgaag atttcgtagt tttcttcacc gacagcatcc
    18721 ttacgaacgt agatctgaat ctggccttcg cggtcttgaa ggtgggcaaa accaactttt
    18781 cctttaccac gtttggttat caagcgtcct gcgatagtag ctgtttcgtt tttatcgtgt
    18841 aattgttctt tatcgaggtt ggcatattta tcttttaatt cttgtgaatt tgcagtacgt
    18901 tcaaaacgtt ttccgaaagg atcgattcct tgttcgcgga gcgcagccat tttttcacgg
    18961 cgaacgatct gctggtcatt tagttcttcc atatgttctg tagacatggg atcctcctag
    19021 ttttactcaa aattgaatac gatgagtcat tttttgcgac actcccattt tatcataaat
    19081 aagcaagaaa ttcacggata atctttaaaa actaaaaaga acctgacgct aaaatcactg
    19141 gaaagaagtg aaagtatctc ccgccccact agcgatatag ccaaatgctg ctttaggaat
    19201 aacttgttgc gccattggct ccaaatcata ggtattgatg aaatctacat gaccttctgc
    19261 attgcttgtt ttgtatgaca taaaatgtcc tccttaataa gtaagcgttt actttgtgta
    19321 ttacaaaaat atcttaactc tttttcaaaa cttttaaaat attttgtttg gaaatttcag
    19381 aaattttatg tctatgataa aaatccttat aacggcaata aaaaatagat attatccaaa
    19441 gaagatttta agtgctacaa taactgtatt atttctagat gggaggttct atttttggat
    19501 tgatccattg ttgaacaata tctaccacta tatcaaaagg cattctttct gaccttgcat
    19561 attgcagttt ggggaatttt gggatccttt ctgctcggtt taatcgttag tatcatccga
    19621 cattatcgaa tccttgtttt ggcgcaagta gcgacagcct acattgaatt gtcacgtaat
    19681 acgccccttt tgattcaact cttctttctc tacttcggtc ttccccgaat cgggattgtc
    19741 ctatcttcag aagtctgtgc aacgcttggg cttgtctttt taggaggctc ctatatggca
    19801 gaatctttcc gaagtgggct ggaagccatc agtcaaaccc agcaggagat tggcctcgct
    19861 attggtctga cacctctaca ggtcttttac tatgtggttc ttccgcaagc aacagcggtg
    19921 gcactcccct cctttagtgc caatgtcatt ttccttatca aggaaacctc tgttttctca
    19981 gcagtggctt tggccgacct catgtacgtc gccaaggatt tgattggtct ctactatgag
    20041 acagacattg cgctagctat gttggtagtt gcttatctaa tcatgctgct acccatctca
    20101 ctggtcttta gctggataga aaggaggctc cgccatgcag gattcgggaa tccaagtact
    20161 ctttcaagga aataatctcc tgagaatctt acagggattg ggcgttacga ttgggatatc
    20221 catcctgtct gtcctcttat ccatgatgtt cagaacagtc atgggaatca tcatgacctc
    20281 ccattctaga atcatacgat ttttaacacg attgtatctg gaatttatcc gtatcatgcc
    20341 ccagctggtg ctactcttca tcgtttactt tggcttggct cgaaacttta atatcaatat
    20401 ctcaggtgag acttcagcta ttatcgtttt taccctctgg ggaacagctg aaatgggaga
    20461 cttggtacgt ggagctatca cttctctccc taaacatcag tttgaaagtg gacaggcact
    20521 cggcttgact aatgttcaac tttactacca catcatcatc ccacaagtct taagaagact
    20581 gctaccgcag gctatcaatc ttgtcactcg gatgattaaa accacttcat tagttgtttt
    20641 gattggggtt gtggaagtga ccaaagttgg acaacaaatc atcgatagca atcgcctgac
    20701 catcccaact gcttcatttt ggatttatgg aaccattcta atcttatatt tcgcagtttg
    20761 ctaccctatt tccaaactat ccactcactt agaaaaacat tggagaaact aaatgtctga
    20821 aactatctta gaaatcaagg aactaaaaaa atccttcgga gacaatccca tcctccaagg
    20881 actttctcta gaaatcaaaa aaggggaagt tgttgtcatc ctagggccat ctggttgtgg
    20941 gaaaagtacc ctccttcgtt gcctcaacgg cttagaaagt attcaaggtg gagatattct
    21001 tctggatggt cagtctatcg ttgaaaataa aaaagatttt cacctagttc gccaaaagat
    21061 tggcatggtc tttcaaagtt atgaactctt tccccatctg gatgtcttac aaaacctcat
    21121 cctaggccct atcaaagctc aaggaaggta caagaaagaa gtaacggaag aagctttgca
    21181 attactagag cgtgtcggtt tgctggataa acaacatagc tttgcccgtc aattatctgg
    21241 tggacagaag caacgtgttg caattgtccg tgccctccta atgcatccag aaatcatcct
    21301 ttttgacgag gtgactgctt cgctggatcc agaaatggtg cgtgaggtgc tggaacttat
    21361 caatgatttg gcccaagaag gccgtaccat gattttagta acccacgaaa tgcagtttgc
    21421 ccaagccatt actgaccgga ttatcttcct cgaccaaggg aaaatcgctg aagaaggaac
    21481 agctcaagcc ttctttacca atccgcaaac caaacgagcc caggaatttt taaacgtctt
    21541 tgactttagc caattcggct catatctata aaggagattc ttatgaaact attcaaacca
    21601 ctcttaactg ttttagcact tgcctttgcc cttatcttta tcactgcttg tagctcaggt
    21661 ggaaacgctg gttcatcctc tggaaaaaca actgccaaag ctcgcactat cgatgaaatc
    21721 aaaaaaagcg gtgaactgcg aatcgccgtg tttggagata aaaaaccgtt tggctacgtt
    21781 gacaatgatg gttcttacca agactacgct acgatattga actagggaac caactagctc
    21841 aagaccttgg tgtcaaggtt aaatacattt cagtcgatgc tgccaaccgt gcggaatact
    21901 tgatttcaaa caaggtagat attactcttg ctaactttac agtaactgac gaacgtaaga
    21961 aacaagttga ttttgccctt ccatatatga aagtttctct gggtgtcgta tcacctaaga
    22021 ctggtctcat tacagacgtc aaacaacttg aaggtaaaac cttaattgtc acaaaaggaa
    22081 cgactgctga gacttatttt gaaaagaatc atccagaaat caaactccaa aaatacgacc
    22141 aatacagtga ctcttaccaa gctcttcttg acggacgtgg agatgccttt tcaactgaca
    22201 atacggaagt tctagcttgg gcgcttgaaa ataaaggatt tgaagtagga attacttccc
    22261 tcggtgatcc cgataccatt gcggcagcag ttcaaaaagg caaccaagaa ttgctagact
    22321 tcatcaataa agatattgaa aaattaggca aggaaaactt cttccacaag gcctatgaaa
    22381 agacacttca cccaacctac ggtgacgctg ctaaagcaga tgacctggtt gttgaaggtg
    22441 gaaaagttga ttagtcatta actcttaaaa ggaactggat tttaagctcc aatccctttt
    22501 taagatttta cctataacat cctgagtcta tctaagatgt tcaatctgaa cacagtgtac
    22561 atactttatc ttctattgca tatactttat cacataagat acgaatatcc tcttcactat
    22621 gactagcaat caaaattgtt gtcccttttt cactagagag ctttctaaac aatgttctca
    22681 tattttctac acttgattta tccaaggcat tcataggttc atctagtaaa agaatagagg
    22741 gattctccat aattgcttga gcaatcccta gctttttcct catacctagc gaataagttt
    22801 taactttctg gtctttttgc tcatatagac caactatttt cagtgtatca ttgatttcct
    22861 gattaccaac tactcctcgt atgcttgcca aatattgtaa attcttaaag ccactataat
    22921 aatttataaa accaggttct tcaatcaaag ctcccaaatt agctggaatt tttctctcag
    22981 gaacaatatt ttccccattg attaacactt ctccataaga cggactatat aaaccagcta
    23041 ttaatttaaa caatacactt ttccctgagc cattcgcacc agtaattcct ataatttccc
    23101 cctgtttaca actaaagtta aggttttgaa aaacacatgt cttttttaat ttcaactcaa
    23161 tattttttaa tgtaattatt tcattcattc tataaacctc ctcttttgac gagtgaaata
    23221 gaaaatgctt tgaaaaagaa agactaaaaa tagcaactga agaaataaat ctcgtcctat
    23281 atctccattc cctcgattca aaatataaaa tagataatta gttcgatttc ctacaaatag
    23341 accaccaaac acaatcatga gtaaaaagaa actaacgcaa gcaaagttca tttgtacatt
    23401 cgctcctagt gcgtataaca taaagcaaaa caagactaac acacaagcat tcaaagaatc
    23461 aactataaac aaaactccta tatcccagtt tggtaactgt aacataaatt gaatcactag
    23521 aacttttacc atgattaaaa tgagtccata aagaaaacag gtccaaatct gatacctaat
    23581 ccagagctta atagaagaaa atcgaatcac ttccataaag aaatcggatg ctcttctacg
    23641 atatacaact gctagaaaaa atgtatgaat aataaaccaa tgaagcaacc atatagataa
    23701 gactaaaaaa gagttttgac tcagattagg cccttttaaa atttctataa caagtctatc
    23761 atttccttct aaccaatacc ataatcctaa acaaatcaat atccaaaaaa gaacataacc
    23821 ccaaaagtac ttggtataga ttatctgatt caacctccac atgtgactac ttaactttcc
    23881 catttccaac ttttacactc tcctccagtt tctatgaaca gaaaatacag tcaatggaat
    23941 tatgatagat agtaatgtaa gccaataaat caatgcatac ggattggaat ttaccatata
    24001 ctgaaaaatt ggcaataatc caattatccc attgtccaac tgataaatcc agaataagga
    24061 ttctacaaat aaaaaagtaa acagaaatac taagctcatg tttgattttt taaaccaaga
    24121 aaaactgcta atagtaagta aggataaaaa gaaatagtat gctatataag agaaaaaaaa
    24181 tcctataaag aaactagcat tgtttgcaat acttatacca taaaattctc ttaaaaaatc
    24241 aacctccttt atctccaaag agaagctaaa accattacta aatgcaatta gaaaaatcaa
    24301 taaaaataaa gtcgccgtcc aaatccccgt actaagagct gctaatttga aactaaaact
    24361 ggtaaagtgc ttaattgatt tcagacgaat gcgacactcc aacctattaa aatagttatt
    24421 catcaaataa aaaaagaaca atatatatgt gaacggaaag caatatactc cagtcgtcat
    24481 atcttgaagt aaaactaaga tccattctaa tacatttgga tggattgaat attggcgaca
    24541 gcgcaataaa tatactgtac tagataaaac acaggatagc agtaatataa aataaaccaa
    24601 tactgctaag aaatcttttt tataaaattg aacaaattgt ttcattatac atagtcctct
    24661 gaatgtagaa aaaatgtacc ataaacaacc aaacaactaa caaataaaat aaaagcaaga
    24721 tgagcactaa ctaaggaaag actgatatct ttctgatatc ccaaagctag cgttgtcaca
    24781 ggttctaagt aagatagccc taaaatagcc caaaaaatac caccaaccat catataggca
    24841 actgggatga aaatagctcc tatttttttc ttcactagca aagcactagc tagtccaaaa
    24901 atagagaaca cagcgcccca aactccatac cagagagtcg ttacaagact atagagaaac
    24961 tgattggaat caaataattc ttttaaggca ccactataat ctccaatata aatttcctga
    25021 taaggagtca ctaaaagatt aattcctaat aataataaat aggggagaaa aaagactaga
    25081 aaagaagaaa taattacagt actacccaca atagccagat acttcttttt agaaattcga
    25141 cacaattgtg ctgttagaaa atgactctca gcatcctcta ttatctgact agaatagggc
    25201 agtgtacaga taagtgcagc tactaggcta atcggtgaaa atcctcgaat agaagaagcg
    25261 gcaaaaaaaa tcgataaacc ttcaatttta taaataccat tgaaagcaag gaaatttcct
    25321 aaactcatgc aaagaagggt caaaaataaa gacatataaa atcgaggtga ttgaacgact
    25381 cgtacaagat tacaaatgaa aaattccatc cttactcctc cttataataa aaatagggtg
    25441 tagcattctt ttttcatgct acacccacaa tcaaccatct ttaaggctta ctctgacaag
    25501 taagttaata agaatctgga ttccaagaac ctgaagtatg aattcttaca tgatttccaa
    25561 attgtggcgc catagctaat ctagtaccag aaccaatata attgtcacca cctccattat
    25621 agtacatgac aatcctagag ccagacccca atgaatatac cggggtaata tctgacccac
    25681 tataggcgct acgaatagag gtatttaacc ttttaccgcc accagtgctg tcattattat
    25741 taattccagc agaggagttt tctttctcaa gctcactagt tgtatccgaa ccccaacgcg
    25801 gcgacgttaa atcataatct ccaatctgtc tagctaaaac tgtagggaga aacaaactag
    25861 atgcatttgt aactacgcaa aaaaggaata tatttttctt cttttcatga catcgtcctc
    25921 ttaagaagtg ataaacaaga tagttgattg atcaatttga tacatattac attttcatta
    25981 tatcaacaaa agaaagcgtt gtcaattatt ttatttaaaa tattgtcata ttttaaataa
    26041 aatggtaaaa gagactcggt agcctcgtct ctctttatct tactgaccta accttcttgc
    26101 caattttctt ggtcttcttt aaagcgtttt agaagatcaa ggccctctgg cgtgatgtaa
    26161 ccttcttctt gggctagatg gataagctcg ctatagtttg aaagcgtcac aagtttaaca
    26221 ccagcatctg caaagttctt atctgctttt ggcaattggt agctgaaaat cgctacaact
    26281 ccaagtacat ctgctccttc tcgcttggct gctgctacag cttcaagaac tgaaccaccc
    26341 gttgaaataa ggtcttcaac cactaccatt ttttgacctt gagctacgcg accttcgatt
    26401 tgattaccag ctccgtggtc ttttggttta ctacggatgt aggcaaaagg caagtccatc
    26461 ttatcagcaa taatggctcc gtgtggaatc cctgctgttg cagttcctgc aatcacttct
    26521 acttcaggaa aggcttcttt gatagcttcc acaaaaccat tttcaattag ggtacgagtt
    26581 tctggatagg ctagtgtcac acgattatca gtgtaaatcg gtgacttgat accagatgcc
    26641 caagtgaagg gttcctctgg tttgaggtaa acggcttgga ttttcaagag gtggctagcg
    26701 atatctttag caagtgtcat ggtattctcc ttttattttt ctaatctagt tctttaattc
    26761 cagtcctgtg tccattcatc cttgatggca tgataagctg caacaggatc ctcagcttgg
    26821 gtaatgggac gtcccactac gatatagtca ctgccgattt gataggcatc agcaggtgtc
    26881 atgactcgtt tttgatctcc aactgcaaca ccagctggac gaatccctgg tgtcagacag
    26941 ataaaatctg gattggtagc ctgcttgatg acttgtactt cctgagccga gcaaacaaca
    27001 ccatccaagc cagcttcagc tgtcttcttg gcatagtgaa tcacagactc ttgcagactg
    27061 gtttggatat tttgaaactc ctgcatctga gcttctgacg ttgatgtgag ctgagttaca
    27121 gcgatcaatt tggcttgact cccaagacct tcacgcgccg ccttcatcat ctctacacca
    27181 ccagccgcat ggacattagt catatcgaca ccaagctgag acaagatctt catggctgac
    27241 ttgactgtat taggaatgtc atgaagtttg agatccaaaa agacactatg acccaaacct
    27301 tttaagtagg acacaatctc aggccccgct gcgtaataaa gctccatccc taccttgaga
    27361 taaaggcttt cttctgctgg gaaaagagct aaaaattcct tgaccgcctc aaaactagga
    27421 aaatcaagag caatgattgg acgatgttct cgcataggtt tctcctttta cctttactag
    27481 tgagagagtc tggccaacag aagccacgaa aaaaggaacc tgccaacagc aaggttccac
    27541 gaaaaatggg tagtctccac cctttcaccg tctaacctta gctgcctctc tggactgctt
    27601 taaaggtttt ttactattct attatttcta ctagcacttg tcaagtaaaa acagctaaat
    27661 ccatgttgac tgctattgaa cacctcacaa gactctttcc aaaagaatag ttaaagactt
    27721 tcacttcgga cagagaaaaa gagtttgctt gttacccttt aattgaacaa atgggaattc
    27781 tgttttattt tgcggatgcc tattcatctt ggcaaagggg aagtaatgag aatgcgaatg
    27841 gacttcttcg tgagtatttt cttaaaccga tttggcaaca attaccgaaa aaatccttaa
    27901 tgatgctttg ctagcttatt aatcaccgtc cccaaaaata cttggattac aagacaggtt
    27961 ttgaagcact ttttgaagca cttatagagg agatttaaaa atgttgcatt ttttattgca
    28021 atttatcagt tatcttatca cccaaaactt ctgcaattag tatgagcact attgttattt
    28081 ttcttttttc gtacactcaa atcaaaatat agcaataaga ctattctgta gaattgctgt
    28141 caaaaagatc agttcatttt ttccttccca cttgtgttta taatcattaa aataactcaa
    28201 ttaagaaatt ttctttttcc taatagttat cgtaattaaa gttaatagaa gaacaatcca
    28261 aataagtagt gttagattag gaattagagt tttattccat gaaaattcct cccattttgt
    28321 gaaaaactta gtaaatagtc ccataaaact ataatctgtt atcttcgcaa ctgtcttatc
    28381 caaaccagat agcatcggta agaaagcaac taaaatcata gcaggaagac tgataacctg
    28441 agctactact tggctcttcg cggttaaacc tatcaataaa tagaataaaa tgatggataa
    28501 agaggttaga agaacgattg taatataatt aaaagtatgt acaattgtaa ctcctaaaat
    28561 aagaggagta gttcccataa tcacaaaagt tagcaaaaaa ggaagaaaca tagttgataa
    28621 aatgtattcg gagcctttaa caccactcaa cagaagagtt tgtaaattgt acttttcttt
    28681 ttcttcagac aagataatag ttataggact tccaacagcc aaagaaaaag aaaaaggtaa
    28741 acacatcatc aaaagaacta atgcctgttg atcgttgacc ttcccctgtg tttccataag
    28801 atatttataa aaatatgtga aagcaaaagg cactaaaact tgcaataaaa tactcttatt
    28861 ggaaaggatg atttgacaac gcaaccatac caaagccaga aaccgtttaa gcatttagct
    28921 ttcctcctgt taatgtgata aaaatatctt ctaaagtagg ctcacatgaa tgaattgaaa
    28981 aaatattttc cagatcagtc tgagatacct gttctagtga tgtaaaatca aaagttatct
    29041 gattcccatt ataatctgta accttaatct ttttatcttt attatatctt tgaatgagtt
    29101 cagaaggagc tccttgctca actaatttcc ctttatttaa taaggcaaca taatcacata
    29161 aaagagttgc ttcattcata tcatgagtcg ttagaaaaat cgttgtccct gctgttttca
    29221 attctaaaat taactcatga attgttcgag aagttgtggg atctagacct gaggtcggtt
    29281 catccagaaa gagtacagcg gggttgttga taagagctcg tgctaaaagc attcgttgcc
    29341 tcattccagt ggataatttt cctgctacca tcttgcgact atcatataat cctactcgct
    29401 ttaacaaatt atcaacacgt gatttactaa tatt
//
LOCUS       DAVHEX010000021        28640 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVHEX010000021 DAVHEX010000000
VERSION     DAVHEX010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28640
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.021"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(<1..1505)
                     /locus_tag="VVL45_000924"
     CDS             complement(<1..1505)
                     /locus_tag="VVL45_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEU4187116.1"
                     /translation="MIYLTEGNTPLNEAYNDEIVHLGNNTYQLTFRFPTSDTKWELLK
                     EETFLTADDLHGEQDFYIFEVEKQQGYIQVYANQVISLLNNYIVSSIEVDRVSGTRVL
                     SAFAGSITRANPFSFFSDIDDRHTLNIKDKNAMEVLAKGKHSILGQWGGDMVRNGYNL
                     RLLKNGGSENESLFMYKKNLSSYQHKTSTKSLKTRITFKTTVKGEGENAVDHDYMVVI
                     DSPLLGNYSQIYEDVVEVNDQDVTDEASLIEYGKQYFRTSMCDMLEDNLEISVVGQSD
                     VAVQMFDVVSFYHEWYGLDVRKKITKYTYSPMAKLLKSIGFGTFQSSLANAIGGIVND
                     AVLNESRNLHQIFEERLKKEIANADRAFDAEFSKREKTITDAIELAKAKAEEVKQELS
                     DTINQRFNSFDNGPLKEAKRRAEEALRNAGASSLLAQEAKRIGLDSVARLEEFKSQTT
                     SAQTALSGDLDALKRTIVNDIRPKQAQVEAEIAKQVEALVQTKKELSGASTL"
     gene            complement(1502..2200)
                     /locus_tag="VVL45_000925"
     CDS             complement(1502..2200)
                     /locus_tag="VVL45_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEU4187117.1"
                     /translation="MFYMIINGFNTSTIPHCVVTDFGQVEAAKPRVVEDANLYGANGS
                     YRVLDGGYESYERTFSFYIPKLLDVSTIVEKFQPKDNVLEFSYQLGSVFYADFIGATY
                     SPHGMHAWKLEIKLNMQPFRYQKNVAPLIFTASGNINNPGSVYSEPVIEIEGDGDISL
                     TIGRTTMHLTVRRKVTIDCRHKKQNIYNADGAVQNTLRKRGGFFELAVGNNSLVFTGS
                     VRKVTVRPNWRYIL"
     gene            complement(2200..6375)
                     /locus_tag="VVL45_000926"
     CDS             complement(2200..6375)
                     /locus_tag="VVL45_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEU4187118.1"
                     /translation="MASGTPLGAMYIELGLDVSNFNPTLTGAKNAVKYFQNTVRSLDS
                     TLKGNEKNASLLQAKYKTLGQAIDSQRKVLDEMKKSFDKLEPGTAKFDKAAADIQREN
                     AKLAAMEGQLRGVEKALQDVGRENSWSGKMDALSEKFAKGGKKLRAMGDAMKPVSAAL
                     LAGFTLSTKKAIDFESQMNTTKSLLADTIPTADELNSTTQKLGESSKNWAKQYGISTA
                     SINEGMQEIIKKGFDANQTIEAMPAILDAAKASGEDFNTVMNATTNILQQFGLSTQDT
                     ERVTNSLTFVANKTAAGFADMGAAMEYVGPVAKNVGMDLEETAATVGLLSNNGIAGEK
                     AGTALRGALTRLLKPSKQNANAMAKLGFSAEEFRSGAIKLPDILDRIKKNTEGMTDAQ
                     KSALIATAFGTEAQTAMNILVDQGGDALRNLANETKGATTYTKDLANELSKSSKNGVE
                     RFKSSLEVLQINIGQKLLPLLTPVIEKVNQFIDWLSKAPPAVQSLAVGIGGFLALGYP
                     LLNFLGNGATALSILIKHAGKLGGLLKGGIGLAKLTERAGKLKGLLQGGIGLAKVGSE
                     ATVLAEGAGVAASSSGLLSGAIGALTNPIGLLVGGVALLAGGLVYLGHKKDEARIKAE
                     EFGSQLSDTARKELRDFQSKVDETSKAVANFGTHAGDAEKVSGAFKKLYDEIAAGAEK
                     SNQRMQELTGKWGLSEEDLAKAREKNAQMVSNAESMMNKINDIYQRHNGDASKFSQEE
                     KEIILNNQREMIKTRISMMNLSAEQQKAALQALNGEIGSLNETQLKHTRDVLKKSLDE
                     EKQLYETSKSELKELLEGKVIDQETYNKKLQTLETNHQQTMEALGTKYYQVMQNLDAK
                     VKARTGQSWNYWEEAKKVLEEYGLSYEVIGQKAAEASQKMGESHSILAKYTSEMSKET
                     KEANDAWSLLVGNINENGNFEIKSNVKEVIGEATKSAEGWEQLQFIVKSANINSNARV
                     MIAEALVESGKWSTMTLEEKQLIVQNQAGLQAIFDSENHLRIWNSMPAEVKQLLLENT
                     DVMNKAEEASKALYNYDALTPKQKELLATDENFRKAVARSTDTLTTWNATTPFTKDLK
                     LDPTNVLNNGQLSIDKIMVWNLSNPETKSLNATDNTSEAVDSAKASVNSPKQEKPIDL
                     LAKDQTAGVRNETSAAINAIKQNNPVDILAKNSTSSTVSEVKSGVNSIQDKTVTISAR
                     DNASGVLAGIRSWIIRVTGDFFTNVFASRHAHGTNYHPGGLAMVNDQRNSTYKELVTL
                     PDGRSFIPEGRDVLLPLPKGAKVLRADKTKRLMREMGVPKYAAGVGIPSDAKFLREME
                     EAQAKIVVQAKGETNSKDSDKVVSEIAILRASMEKILTAILEKPSDTYLDADKISMSV
                     YQRQGAIYAREGM"
     gene            complement(6432..6662)
                     /locus_tag="VVL45_000927"
     CDS             complement(6432..6662)
                     /locus_tag="VVL45_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187119.1"
                     /translation="MSRKEKARRRYWFWFTIFILYLAFGLYCICTNFGDTLGMLLLSP
                     FIFASLPLYAYLFLGLFIWAFMSLAMDDFYKE"
     gene            complement(6911..7204)
                     /locus_tag="VVL45_000928"
     CDS             complement(6911..7204)
                     /locus_tag="VVL45_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187120.1"
                     /translation="MAKVQFTIKNDKGEDVLKKSKEITTRDYRDYLVMNDSLTSDLSE
                     VEKLDKQLGFIASLFDDVTVEQLLEYTDFAKVISIFTDIYAHLVGDVDPKEKS"
     gene            complement(7253..7849)
                     /locus_tag="VVL45_000929"
     CDS             complement(7253..7849)
                     /locus_tag="VVL45_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEU4187121.1"
                     /translation="MTVKGTALIGLKSVTIRVHDGQTPTVGQNLFTLEGKDNEGATQT
                     ARVTGLSSDPVKTYGSNVAYHVSNRGVGDVKVEMGLLDVPLALYTNALGYGDDDGIYY
                     FGADTVAKNVSILIESNTADGEPVYYGFYKGQLSMDAIDFETIKDKANELATTNVNFA
                     ATASSDTATKGRYGAIVYGSDAEKLKKLKGQLNMAAAG"
     gene            complement(7866..8246)
                     /locus_tag="VVL45_000930"
     CDS             complement(7866..8246)
                     /locus_tag="VVL45_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187122.1"
                     /translation="MIKDKLTELYDALEEDESLSGISIKSFERPESLGDDETSIVIIP
                     VGPPMQTAHGSNTSLAKTFLYQINVESTNRVECKELQGRIEKIMEEQGFYQTEGGLEQ
                     WIPDIKRYVDVRTYRGMSALYEKY"
     gene            complement(8246..8644)
                     /locus_tag="VVL45_000931"
     CDS             complement(8246..8644)
                     /locus_tag="VVL45_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187123.1"
                     /translation="MSSGANLKGFDDILRNIEARLGEPVVRRNVNRALKETVEEFEPT
                     FKRAMAVYADTGKTVGAVVHGNVTGTSSGVPMVKLGFKSPRWTLIHLNEFGYAKNGHP
                     RGFGVMRRFFEASKPVFKSKVGMKLKQEFL"
     gene            complement(8637..9026)
                     /locus_tag="VVL45_000932"
     CDS             complement(8637..9026)
                     /locus_tag="VVL45_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HEU4187124.1"
                     /translation="MKRSEGPSYRYKKPESQNGDLRTPLTFYTSKIKKGVDGRDVSYE
                     KAFYTMGQTYSPSMKDVEIATGKAMKAKMTLKIRDPLTDYQPENRHFVEVGDIRLVGK
                     KWQIIDVRPDYDNRDFLIVLIGGGRDV"
     gene            complement(9013..9201)
                     /locus_tag="VVL45_000933"
     CDS             complement(9013..9201)
                     /locus_tag="VVL45_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187125.1"
                     /translation="MIKVLKEFYDLKEGVLRQVGGRFEATEERFAELKEHLPGYVELE
                     KSSRSRSSKEVVSVNEEE"
     gene            complement(9194..9484)
                     /locus_tag="VVL45_000934"
     CDS             complement(9194..9484)
                     /locus_tag="VVL45_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEU4187126.1"
                     /translation="MEIEAKLHSLLDPFKERMRIFHSGEDANLSRMLESSQMVITRLV
                     GSGNIHDHQIRELILERARYVYNDQVEFFYENFKADILALSLDKIEMEDSDD"
     gene            complement(9498..10613)
                     /locus_tag="VVL45_000935"
     CDS             complement(9498..10613)
                     /locus_tag="VVL45_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEU4187127.1"
                     /translation="MMKLGNEFTTARQNFMNAVETGAPMEEQNKLYNEMIEALTDKLQ
                     DDAREAARKEIATMNPYDAQLTAEAREFFNNIEKLPPKGIEKLFPQETIDRIFEDMVA
                     ARPLLQHIGLRNAGIRLKFLKSSRTGQAVWGKINAEIQGQLKQEFAEEEAIQSKLTAF
                     VVIPKDSEKFGPAWLQSYVSIQITEAFAAALEAGYLNGDGDKQPIGLSRTLTGTVESE
                     KVTYAEKQAQKKPLTFADSATVVKELTEVYKYHSTKADNTTPVAVEGNLVMVVNPVDA
                     WDVKKQYTSLNAQGVYVTAMPYNLILVESVAQTSGKVTTFVKGRYDAFVGGGIEFGRF
                     TETYALEDLNLYTAKQFAYGKAHDEKTAAVWTLNIGK"
     gene            complement(10632..11354)
                     /locus_tag="VVL45_000936"
     CDS             complement(10632..11354)
                     /locus_tag="VVL45_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEU4187128.1"
                     /translation="MTIIQIKGAIVSNDDRWFYDWIDMDATAPKDIVLPTTGEDVEVH
                     INSGGGDVYAGSEIYTALRAYQGHVTVKIVGIAASAASVIAMAGDQVEISPTAQIMIH
                     NVSAGVRGDHKALLHEAGVLEGFNKSIAYAYIDKTGKALDDLLDLMEGETWFDARSAV
                     NHGFADKVMFESQEAPVLVASQTPVIPRDFIEKIRSAMTPDVELLADLVRDRLNNSLL
                     IPEDKKKEDKTAEPVGLGRFPF"
     gene            complement(11335..12483)
                     /locus_tag="VVL45_000937"
     CDS             complement(11335..12483)
                     /locus_tag="VVL45_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEU4187129.1"
                     /translation="MGFLNLFKRDAPDVGFDFEELERMLGRLQLKSLAIDKSAEFVAR
                     IFAKSEFRYMVENHHVASDWDYILNVRPNKNESASEFWQKVIYRLLTKNEVLIVLSDD
                     KQLLVADAFTRRRYALYDDTFEMVSVGDYIFQRKFAMSDVIFLQYNNNRLQEYVSDLF
                     SDYEKLHTRLVEALARNNQIRGVLATKANGTFDDDKRKKLQRYADNLFKSFTSKTVAI
                     VPTMDGVEYSELTNTVGTTNLSVEELKKLRRQFDDEVADILGLPTALMHGDMADLSNS
                     QKMFNSYCYESLVKKVGDALNHAMVSSAGYANQKKFVIFGEGGLDKFALAENIDKLIS
                     SGAMTRNEVRLELGLEAVPGGDSFLITKNYQGENEIEKGGNEDDNHSD"
     gene            complement(12540..14180)
                     /locus_tag="VVL45_000938"
     CDS             complement(12540..14180)
                     /locus_tag="VVL45_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEU4187130.1"
                     /translation="MISHPLVDDYIKMAESGEIVVNKERKLLFKIIKEKIYPRDDLYF
                     DNDLIDKFIRFAEKNFFPLAKYQLFLTPFIFLFRKEDGEPHFDEYLYTLARGGGKNGF
                     MSARSSFFISPIYPIRDYDVTITANSEKQGKVSFEEVYETIQRRGLEDHFYLTKMSIT
                     GRANNSVFSFRTNNPKTMDSARDGCLEFDEIHQFEDDKAVKVQRSGLGKIAHARTFYN
                     GTNGYVREGFYDKLIEKSMQILNGEVDDFRLFPFICKLDSADEVDDMKKWPKANPMLD
                     ESTPYAKRLLARTKADYDDLELEPSGRQEFMTKRMNLPEADLEKDVTSREKLVACLRS
                     PGIDLLGKSCVAGFDYASIRDFASVGLLFKNGDEFIWKQHSFARKVFLKAFKLKAPIQ
                     EWADKGLFTIVDGPSIDPRLLVAKLEEWSKFYQIELVCADGFRMDLLKPLLEEAGFEY
                     EFLRNPGAIQSKVAPIIEDGFANERFVFENDNSMIWYTDNTYIKEDKDGNKRFLKKEP
                     VRRKTDGFHALIAALYKRELVHESNVGEFLDMLDSWDF"
     gene            complement(14177..14515)
                     /locus_tag="VVL45_000939"
     CDS             complement(14177..14515)
                     /locus_tag="VVL45_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187131.1"
                     /translation="MEEYSEKNIKELENQLLSKIGYFSPRKKDAIQYEKVNRYLYLVR
                     LLYELKAKLHEDGLVITVHNGQQRFQKANSLIKEINTTSNQLLAIERSFDFEVENSPV
                     EKPTSGSDLL"
     gene            complement(14634..14930)
                     /locus_tag="VVL45_000940"
     CDS             complement(14634..14930)
                     /locus_tag="VVL45_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEU4187132.1"
                     /translation="MIDVSTREERNQFYNSSEWRTIRRQALKRDHYECVWCRDEGKVT
                     TTNLEVDHIKELELYPEFALDIDNLRTLCKACHNKRHDRFDKNDRNFRKDEWWG"
     gene            complement(14927..15133)
                     /locus_tag="VVL45_000941"
     CDS             complement(14927..15133)
                     /locus_tag="VVL45_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187133.1"
                     /translation="MKGIIKRLFNKRTTKQKPLGKIVVGVEIENRSELKELTQQCCEA
                     IEHLNNCIDKLNKFELKVSTSIIK"
     gene            complement(15299..15865)
                     /locus_tag="VVL45_000942"
     CDS             complement(15299..15865)
                     /locus_tag="VVL45_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU4187134.1"
                     /translation="MVVVEPIRNRDDVQLMIEWLTLHSAVKESDRQRNLMLFLSGVNL
                     GFRIGDIVKLKVKHVKGWHVQIVDEKTDKPTKRKMPKKFKNAMRQYIKDKKDEDFLFP
                     SRNGKHQHIKPNTAYKIIKRAAEEVGLENIATHSMRKTFGLFMYEQTKDVALIMDLLN
                     HSSQSISLRYIGKNQDSQDRAMTKFQGF"
     gene            complement(15952..16377)
                     /locus_tag="VVL45_000943"
     CDS             complement(15952..16377)
                     /locus_tag="VVL45_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000963208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU4187135.1"
                     /translation="MAIDIKKRLKALPYIDIKAKSKHQEIISLKSGILRGQQFDSMPK
                     SKSNKNQTEELNVLIIDKSEQLYKEIKQMYHERDELVQAIESLDDPVENIVMRLLYID
                     GLSWKEVQIKLNCSPATIQRAKHKALLKLSKMYDKNDSK"
     gene            complement(16381..16779)
                     /locus_tag="VVL45_000944"
     CDS             complement(16381..16779)
                     /locus_tag="VVL45_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187136.1"
                     /translation="MIPKFRGLSIDEKSKGEWQYGHLIEDRGRAFIINEVVEANEQYI
                     TIGSWCPVNPATLGQSTGLRDKNGKEVFIGDIVKCTRGCLHEVYLEKEYGGTFIGGMP
                     AVYLKGFGDGYAWTEYEEIIGNIYENPVRR"
     gene            complement(16791..17069)
                     /locus_tag="VVL45_000945"
     CDS             complement(16791..17069)
                     /locus_tag="VVL45_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187137.1"
                     /translation="MKRLGIIIGAVFVIVVSPFVVQYGWNEIITTIVPVGKITVWQAL
                     GMDALLSFIWPVLSSKKESEEDYSYAVKSSISKIITCAFLIWLASLFI"
     gene            complement(17171..18253)
                     /locus_tag="VVL45_000946"
     CDS             complement(17171..18253)
                     /locus_tag="VVL45_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEU4187138.1"
                     /translation="MKFLDLFAGIGGFRLGMESAGHKCIGFCEIDKFARESYKAIHNT
                     KGEIELHDITAVSDESIRGIGSVDIICGGFPCQAFSIAGNRRGFEDTRGTLFFEIARF
                     ASILRPKYLFLENVKGLLNHENGVTFETIISTLDELGYNVEWQVLNSKNFGVPQNRER
                     VFIIGHLRRERTRRFFPLSGKNQSTSNQSVMKIGNINPSGNGMNGEVYQADGLAPTLT
                     TNKGEGQKIAIKSNTIKQFGVLQPNFNQCGVVYETDGIAPTIRAYQGGGLEPKIIQRG
                     HGYNKGGEHDIAPTLTSNSYHENNHLSDGFRIRKLTPRECWRLQGFPDWAFDKAQEVN
                     SNSQLYKQAGNSVTVNVIAAIAKELS"
     gene            complement(18284..18571)
                     /locus_tag="VVL45_000947"
     CDS             complement(18284..18571)
                     /locus_tag="VVL45_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187139.1"
                     /translation="MTQTLEEGMKNKSKCIKVPREIRPFDIGYRIVNKYGQALALRNG
                     ASIFDLPFLAEKAIEKEFGKNDPDFDIGKHSVEEVAIVNLSKFHSYFEERV"
     gene            complement(18571..19158)
                     /locus_tag="VVL45_000948"
     CDS             complement(18571..19158)
                     /locus_tag="VVL45_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEU4187140.1"
                     /translation="MNKDLIETPRFNFFIGDEVLLKGKIVGFDVDENKCVENVVRLEY
                     GQTLNVPNNNIYITDDIVDKSKIKVVVPQFVADWYEENKDSFEFNVWDWIAFRDEAKK
                     SENREFNNWINNSRENPIQTLVNMHQFGYEVEEEKRYTVVTKATKQPLYYNAMDKKLF
                     FSMGGLATKFTRKQLEEVGVGWVFDCPGIEIEEVE"
     gene            complement(19151..19651)
                     /locus_tag="VVL45_000949"
     CDS             complement(19151..19651)
                     /locus_tag="VVL45_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEU4187141.1"
                     /translation="MNKQELIKKLEERRTMTRNFQGYVVWWKDVKEIFEQLGEPQPVK
                     VPQFVADFIAEQKKLGHTLSYSIDASMSDIVAEWYWDNSELFALAWLNGYEVEKEKRY
                     FVKIKGNIKENMLVYGEFLKRYFFTKGFSLDDVIYSHTRKQLEDANFGWVFDCEGIDI
                     EEVENE"
     gene            complement(19653..19970)
                     /locus_tag="VVL45_000950"
     CDS             complement(19653..19970)
                     /locus_tag="VVL45_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187142.1"
                     /translation="MMEELKQKVNAVYNWTVEDGKPQPPKQDLPQVVKDRADYFWEMA
                     EDGMTFMGAMECIFADEKPTDYDLGATKDWLPKSREFDDWIGYSPSMAQVVIAVYLIY
                     GGN"
     gene            complement(20138..20305)
                     /locus_tag="VVL45_000951"
     CDS             complement(20138..20305)
                     /locus_tag="VVL45_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187143.1"
                     /translation="MVGVTYQEIHLFVEFLKEQYGQGSPDYIEALNDLDGLVEVSYRE
                     AIERFLEDEVR"
     gene            complement(20432..20659)
                     /locus_tag="VVL45_000952"
     CDS             complement(20432..20659)
                     /locus_tag="VVL45_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187144.1"
                     /translation="MLELYFVYNGHCKFYLGTFDNVDDLIEQMEDHQWAFSAITHPRF
                     QKHIGQRTTRFDYGSKDCYYLATFSGGEKND"
     gene            complement(20659..20853)
                     /locus_tag="VVL45_000953"
     CDS             complement(20659..20853)
                     /locus_tag="VVL45_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187145.1"
                     /translation="MFILKHGTREDKPFLMSAVIGVTGLDISCSEEKKAMRFVSRGAA
                     VQVGKALRGSFGNFYPVEVE"
     gene            complement(20868..21638)
                     /locus_tag="VVL45_000954"
     CDS             complement(20868..21638)
                     /locus_tag="VVL45_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4187146.1"
                     /translation="MVDNVFEEIALSYRRNTEQQEEFCEKHNIPLIKILRTESVVCRM
                     CESERIHEENQARVNELADAENERERKYYLEKFSLYDEVLKNATLDNFETPTEKEAEK
                     LVFAKRICREWSEGARNNIVLQGEAGTGKSHLAFAMVKALSEYTKEIAIFINVTDLLM
                     KIKADFSQEEFLVNKIASAKFLVLDDLGMEKDSEWSFTILYNILNKRSNTIITTNLIS
                     ADIQKRYGRPFMSRLMKGVDKDHLMVFNDLTNKRKQYF"
     gene            complement(21632..21790)
                     /locus_tag="VVL45_000955"
     CDS             complement(21632..21790)
                     /locus_tag="VVL45_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187147.1"
                     /translation="MGVLELIEQFEIDYYPLSYEKKTLLADQPIHQVVACLSEMASWH
                     ECGGRLVW"
     gene            complement(21778..22578)
                     /locus_tag="VVL45_000956"
     CDS             complement(21778..22578)
                     /locus_tag="VVL45_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEU4187148.1"
                     /translation="MSEIKWIKITTDIFDDEKICLIDALPDPDAILVIWFKILTLAGK
                     HNSNGLLMMTDKVHYTDEMLATIFRRPLNTVRMAIGVFEQFGMIEIIDGIISLPNWEK
                     HQNVDGMEKIKEQTRNRVAKYRKKQKNLALGNVTGNVTVTDGNALEEDKDKNRLDKDK
                     NKKRITTTSSGSEENILELFQSEFRRLLSGFEIEEINHLLNENDVDLVKEALKTAINS
                     GKPNIKYIGGILRNWQMNNVTTVEQVRQSEKKNKDKKEEQEAKDEWGY"
     gene            complement(22571..22867)
                     /locus_tag="VVL45_000957"
     CDS             complement(22571..22867)
                     /locus_tag="VVL45_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187149.1"
                     /translation="MEELIESLDNLIMIVKELEGRESTSRHFITIWENDYKNLLRVKE
                     YLTDYEKLAKDYRDVELKNKLLKIEKMELEGRHIYEDMRMKYRANRRKWGARYV"
     gene            complement(22883..23203)
                     /locus_tag="VVL45_000958"
     CDS             complement(22883..23203)
                     /locus_tag="VVL45_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187150.1"
                     /translation="MFEPPILDQLMGVGALLLGFAGACRHIKLQEQRKEEERREEQEF
                     AYMIIQVRNHAYERGREDKWQEIRKNIRREFKGFTYDNEPPVGLRPELLALPEPKQSG
                     IRFL"
     gene            complement(23289..23546)
                     /locus_tag="VVL45_000959"
     CDS             complement(23289..23546)
                     /locus_tag="VVL45_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU4187151.1"
                     /translation="MDNVLLSLSEWIKSIIKDTITRLVEIEKDSDHYPELMDVNTTCD
                     FLGIKYATFSDNYRYLKGFPKELPGKKWSKRAIKEWLSNQI"
     gene            complement(23559..24272)
                     /locus_tag="VVL45_000960"
     CDS             complement(23559..24272)
                     /locus_tag="VVL45_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HEU4187152.1"
                     /translation="MNEIFNFHGQEVRTLTIDDEPWFVGKDVADILGYSKARNAIALH
                     VDEDDALKQGLTDNLGRVQETIIINESGLYSLILSSKLPQAKEFKRWVTSEVLPAIRK
                     QGGFIREDLDEDAFIALFTGQKKLREQQVTMLEDIDYLKSEQPIHPSYAQSLLKKRKA
                     RVVACLGGIDSPAYADKTFAQSVFRQAEIDFKDHFNINRYDLLPKKFADAALAYWMTW
                     EPSTNTKMKIMELNSFDEG"
     gene            complement(24415..24618)
                     /locus_tag="VVL45_000961"
     CDS             complement(24415..24618)
                     /locus_tag="VVL45_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU4187153.1"
                     /translation="MSKELKIIKAKIKTRLIELDMTQAELAKQVSVASSVISELLKYG
                     KGSESVKEKVADVLGIENPWRNH"
     gene            complement(24630..24776)
                     /locus_tag="VVL45_000962"
     CDS             complement(24630..24776)
                     /locus_tag="VVL45_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187154.1"
                     /translation="MEDKIIELADYFISENTTYREAKIACEKLFKQASHEIELRALES
                     ETKK"
     gene            24895..25116
                     /locus_tag="VVL45_000963"
     CDS             24895..25116
                     /locus_tag="VVL45_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187155.1"
                     /translation="MSKLTKEDVLQVSQEIINDAIPVIQDMLDEVFKEYPIDMEIRKA
                     ILNSVLVAHKLSTETTVSLLTELVNAQEN"
     gene            25494..25856
                     /locus_tag="VVL45_000964"
     CDS             25494..25856
                     /locus_tag="VVL45_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU4187156.1"
                     /translation="MFFTFEKIKELADKQGISLNKLEEKLGFSRNTIYNMKKSTPNVE
                     RVSMIADYFNVSTDYLLGRTDNPTIAGSHDYKWEGKTLNVEEMASNVMMFGGRELTDE
                     KKKIIQSIIEGYLKEAGD"
     gene            25869..26252
                     /locus_tag="VVL45_000965"
     CDS             25869..26252
                     /locus_tag="VVL45_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEU4187157.1"
                     /translation="MTEKEIISHFQIRIIDFDGDLMPDELGFYEKETNTAFLSNKLSK
                     KERVKVLLHELGHKDHTRSEYQNARLRCENEADRNMIHHLVKDAIESLDDPTEFDYLK
                     FMSYYNLKTMTNEVMVKEEYFALME"
     gene            26265..27188
                     /locus_tag="VVL45_000966"
     CDS             26265..27188
                     /locus_tag="VVL45_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HEU4187158.1"
                     /translation="MSYSYVALDVETANDFRGSVCSIGLVKFKDGNIVDSFYTLINPE
                     EEFDDFNIFIHGITPEDVLDSPTFPEVRKAIVDFIGSDIVVAHFAQFDMGALKDVYQK
                     YELDFDNIEYICSYRLAKVALPGQLNYKLKRLAKNLNIELDHHNALSDARASGLILEY
                     LLSTNSFSDLNAFLKEYSYNKTGLLGQYGFKRKKSYQYKENLIYQPTEEEKAAMNPDH
                     YFYGLYFCFTGKLERMTRKEANKATALVGGIPEKGVTKHTNILVVGEQDWRVVGTDGL
                     SSKMKKAQTLLEKGQDIEIMTENDFIRLLEE"
     gene            27367..28515
                     /locus_tag="VVL45_000967"
     CDS             27367..28515
                     /locus_tag="VVL45_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000876726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEU4187159.1"
                     /translation="MKYNKTKYPNIYYYETAKGKRYYVRRSFFFRGKKREKSKSGFTT
                     LPQARAALVELEQQIQEQELGINTNLTLDQYWDIYSEKRLSTGRWNDTSYYLNDNLYK
                     NHIKAKFGSILLKNLDRNEYELFIAEKLQNHTRYTVQTLNSSFMALLNDAVKNGNLLS
                     NRLKGVFIGQSDIPAANKKVTLKEFKTWIAKAEEIMPKQFYALTYLTIFGLRRGEVFG
                     LRPMDITQNDSGRAILHLRDSRSNQTLKGKGGLKTKDSERYVCLDDIGTDLIYYLIAE
                     ASKIKRKLGIIKEQHKDYITINEKGGLINPNQLNRNFNLVNEATGLHVTPHMMRHFFT
                     TQSIIAGVPLEQLSQALGHTKVYMTDRYNQVEDELAEATTDLFLSHIR"
BASE COUNT         8195 a         6765 c         4703 g         8977 t
ORIGIN      
        1 agggtacttg cgccagacag ttcttttttg gtctgaacaa gtgcttcaac ttgcttggca
       61 atctcagctt caacctgtgc ttgcttcggt cgaatatcat tcacgatagt ccgtttcaga
      121 gcgtccaagt cacctgacag agccgtttgt gcgctcgtag tctgcgactt aaactcttca
      181 agtctagcga cagaatccag cccaatccgc ttcgcttcct gagcgagtaa gctgcttgcg
      241 ccagcgtttc tcaaggcttc ttcagccctg cgcttagctt ctttcaatgg cccgttgtca
      301 aagctattaa agcgctgatt gatagtgtca gacagttctt gcttgacttc ttccgctttg
      361 gccttggcaa gttcgatggc atccgtgatg gttttctcac gcttggaaaa ttcagcgtca
      421 aaggcacggt cagcgttggc gatttccttt ttcaaacgtt cttcaaaaat ctgatgcaga
      481 tttcggcttt cattcaaaac ggcatcattt acaatcccac cgatcgcatt tgcaagactg
      541 gactggaagg ttccaaaacc tattgatttt aggagttttg ccattggcga ataggtatat
      601 ttggtgattt tcttacgaac atcaagaccg taccactcat ggtagaagct gaccacatca
      661 aacatctgca ccgcaacatc actctgaccg acaaccgaga tttcaaggtt atcttctagc
      721 atgtcgcaca tacttgtccg aaaatactgc ttaccgtatt caatcaagct agcctcatct
      781 gtcacgtctt ggtcattgac ttccaccaca tcttcgtaga tttggctgta gttcccaagt
      841 aaggggctgt caatcaccac catataatca tggtcaaccg cattttctcc ctcgccctta
      901 acagtcgttt taaaggttat ccgagttttt aaagacttcg ttgaggtctt atgctggtag
      961 ctggacaagt ttttcttgta cataaaaagc gattcatttt cagaaccgcc attcttcaac
     1021 aagcgtaaat tgtagccgtt tcgcaccata tctccgcccc actgaccaag gatagaatgc
     1081 ttgcctttgg ccaagacctc catggcattc ttatccttga tattgagcgt atgcctatca
     1141 tcaatatcag agaagaaaga aaaaggattg gctctggtaa tactaccagc aaaagcactc
     1201 aacacccttg tcccactgac acggtcaacc tcgatagagc taacgatgta attatttaac
     1261 aaactgataa cctgattggc atagacttga atatatcctt gttgcttttc aacctcaaaa
     1321 atataaaaat cctgctcacc atgcaggtca tctgcagtca gaaaagtttc ctctttcagt
     1381 aattcccact tggtatccga tgtaggaaaa cgaaaggtca gttgataggt attgttcccc
     1441 aaatggacaa tttcatcatt gtaggcctca tttaaaggtg tattgccttc agtaagataa
     1501 atcataagat atacctccaa ttcggccgaa ccgtgacctt gcgaaccgaa ccagtaaaga
     1561 ccagactgtt attaccaact gccaactcaa agaagcctcc acgtttacgt aaagtatttt
     1621 gaaccgcgcc atctgcatta tagatattct gtttcttatg cctacaatca atggtcactt
     1681 ttcgtctaac ggtcaagtgc atagttgtcc ttccgatagt caaagaaata tcaccgtctc
     1741 cctcaatctc aatcacaggc tcgctataga cagagcctgg attgttgata tttccacttg
     1801 cggtaaagat aagaggagca acatttttct gataacggaa cggttgcatg ttcaacttga
     1861 tttctagttt ccaggcatgc atcccatgag ggctataggt tgcaccaata aaatccgcat
     1921 aaaatacaga ccccaactgg tagctaaact ctagcacatt gtccttaggc tgaaatttct
     1981 ccacgatagt agaaacatcc agtaacttag gaatatagaa cgaaaaagtc cgttcataac
     2041 tctcatagcc accatccagc actcgataac ttccgttggc tccgtagagg ttagcatctt
     2101 caaccaccct aggtttagct gcctccacct gaccaaaatc cgtcaccacg cagtgaggga
     2161 tagttgatgt attaaaccca ttgataatca tataaaacat tacattccct ccctggcgta
     2221 aattgcacct tgacgttggt agacgctcat tgaaatttta tcagcgtcta ggtaagtatc
     2281 tgacggcttt tcaaggatag cagtaaggat tttctccata cttgctctca gaatcgctat
     2341 ctcagacacg actttatcgc tgtctttgct atttgtctct cctttggctt gaaccactat
     2401 tttagcttgt gcttcttcca tctcacgaag gaatttagca tcactcggaa tgccaactcc
     2461 tgcagcatat ttaggaacac ccatctcacg catcaagcgt ttggtcttat ccgctcgcaa
     2521 aactttagcc cctttaggaa gaggaagaag aacatccctc ccctcaggaa tgaagctcct
     2581 accatctgga agagtaacta attccttgta ggtactattg cgttggtcat tgaccatagc
     2641 aagaccacct ggatggtagt tggtaccatg agcatgccga ctagcaaaaa cattcgtaaa
     2701 gaaatcacca gttaccctaa taatccaaga cctaatgcct gcaagcacac cagaagcatt
     2761 atctcgagcg ctgattgtaa ccgttttgtc ttgaatacta ttaacgccac ttttgacctc
     2821 gctaacagta ctggaagtgc tatttttagc aaggatatct actggattat tttgcttaat
     2881 cgcattaata gccgcactcg tctcatttct aacgcctgca gtctgatctt tcgcaagtaa
     2941 atcaattggt ttttcttgtt tcggagagtt tacgctggct tttgcactat caactgcttc
     3001 actagtattg tcagtagcat ttaatgattt agtctctgga ttagatagat tccaaaccat
     3061 gattttatct attgacaatt gcccgttatt caaaacattt gtaggatcta gtttcaaatc
     3121 ttttgtaaac ggtgtggttg cattccaagt cgttagcgtg tcagtcgaac gcgcaacagc
     3181 ctttctgaaa ttctcatccg tagccagcaa ctctttctgt ttaggagtca aggcatcgta
     3241 gttatagaga gctttggaag cttcctcagc cttattcatc acatctgtat tttccaaaag
     3301 gagttgcttg acttctgcag gcatactgtt ccagattcta agatggtttt cactatcaaa
     3361 gatagcttgc agtcctgctt gattttggac aatcaactgt ttttcttcca gtgtcatagt
     3421 tgaccactta ccagactcaa ccaaagcctc agcaatcatc acacgagcgt tagagttgat
     3481 attcgcactt ttaacaataa attgcaattg ctcccaaccc tcagcagatt tagtcgcttc
     3541 cccaattact tcttttacat tggatttaat ctcaaagttg ccattctcat taatattacc
     3601 aacaagtaaa gaccaagcat cattcgcttc ttttgtctct ttgctcattt cgctagtgta
     3661 tttagcaaga atactgtgag attctcccat tttttgagaa gcttctgctg ccttttgacc
     3721 gattacttca taagacaagc catattcttc cagaaccttc ttcgcttctt cccagtagtt
     3781 ccagctttgc ccagtccgag cttttacctt agcgtcaagg ttttgcataa cctgataata
     3841 tttagtccct aaggcttcca tagtttgctg gtggtttgtt tctaaagttt gaagtttctt
     3901 gttataggtt tcttggtcaa taacctttcc ttccagcaac tctttcagct cacttttaga
     3961 agtctcataa agttgttttt cttcgtccag agacttcttc aaaacatctc gtgtatgttt
     4021 caattgcgtc tcgtttaaac taccaatttc tccattcaaa gcctgaagcg cagccttctg
     4081 ttgctctgct gacaagttca tcattgaaat tctagttttt atcatctctc tttgattatt
     4141 caagatgatt tctttttctt cttgagaaaa cttactagca tctccattgt gacgttggta
     4201 aatatcattg attttgttca tcatgctttc agcatttgaa accatttgag cattcttctc
     4261 tctagctttt gcgaggtctt cctcactgag accccatttg ccagtcagtt cctgcattct
     4321 ctggttagac ttttcggcac cagcagcaat ctcatcataa agctttttaa aagccccaga
     4381 gactttctca gcatctccag catgtgtacc aaagttagca actgctttgc tggtttcatc
     4441 taccttactt tggaagtctc ttaattcttt ccttgcagta tcactcaact gagaaccaaa
     4501 ttcctcagcc ttaatacgcg cctcgtcttt cttatgccct agatagacca atcctccagc
     4561 tagtaaagca acaccaccca ccagcaaacc aatcggattt gtcaaagccc caatagctcc
     4621 agacaagaga ccagaactag atgcagcaac accagctcct tccgctaaaa cagtggcttc
     4681 acttcctacc ttagcaagac cgataccacc ttgtaaaagt ccttttaatt taccagcacg
     4741 ctctgtcaat ttagcaagac cgataccgcc ctttaaaagt cctcctaatt taccagcatg
     4801 ttttatcaaa atactaagag ccgtcgcacc attgcctaga aagttcaata aaggatatcc
     4861 tagagccaaa aaaccaccaa taccaaccgc caagctctga acggctggag gagccttact
     4921 taaccagtcg ataaattggt tcaccttctc aatcacagga gttagtaagg gtaataattt
     4981 ctgaccaatg ttaatctgta aaacttctaa actagattta aaacgttcca caccattttt
     5041 agaagactta gataattcat ttgctaaatc cttagtataa gttgtcgccc ccttggtttc
     5101 gttagcaaga ttacgcaaag catctccacc ttggtcaacc aagatattca tcgctgtctg
     5161 agcttctgta ccaaaggctg tagcaatcaa ggccgatttc tgagcatctg tcattccttc
     5221 cgtattcttt ttgatacgat ccaaaatatc aggtagctta atcgcaccac tacgaaattc
     5281 ttctgcagaa aaaccaagtt ttgccattgc atttgcattt tgcttagatg gtttcagcag
     5341 ccttgttaaa gctccacgaa gagccgtacc agccttttca ccagcaatac cattattgga
     5401 caaaagacca acagtagctg cagtctcttc caagtccata ccaacgtttt tagcaacggg
     5461 accaacatac tccattgcag cccccatatc agcgaaacca gcagccgttt tattggcgac
     5521 aaaggttaaa ctattggtta ctcgctctgt atcctgagta gatagcccaa attgttgcaa
     5581 gatattcgta gtggcgttca taaccgtatt gaaatcctca ccagacgcct tagcagcgtc
     5641 taggatagca ggcatggctt cgattgtttg attggcatca aaacctttct tgataatttc
     5701 ttgcatccct tcattgatag atgcagtaga aatcccatac tgtttcgccc aattttttga
     5761 actctccccc agcttttgtg tggtactgtt cagttcatcc gctgttggaa tagtatcagc
     5821 taacagagat ttagttgtat tcatctgact ttcaaagtca atcgccttct tagtcgaaag
     5881 tgtaaaacca gctaataaag cagcagatac aggcttcatt gcatcaccca tagcccgcaa
     5941 tttttttcca cctttagcaa atttctcact caaggcgtcc atcttaccag accaactatt
     6001 ttcacgccct acatcttgca aagctttttc aacaccacgt agctgacctt ccattgctgc
     6061 taacttggca ttctcgcgct gaatatcagc agcagcctta tcaaacttag ctgtccccgg
     6121 ttcaagcttg tcaaaacttt tcttcatctc atccaaaact ttacgttgtg aatcaatggc
     6181 ttgtcccaaa gtcttgtatt tcgcttgaag taagctagca tttttttcat tcccttttaa
     6241 agtactgtcc aaagaacgga cagtattttg aaagtacttt acagcgtttt ttgcaccagt
     6301 tagagtagga ttgaaattcg acacgtccag ccctagctcg atatacattg ctcctaacgg
     6361 cgtaccgctt gccattttgt tctccttcct aactcctcag agcaaagaaa aaagccctta
     6421 cggacttttc atcattcttt ataaaaatca tccattgcca ggctcataaa agcccagata
     6481 aacaaaccaa gaaatagata agcgtagagc ggcaaggaag caaagataaa gggtgataat
     6541 aaaagcatac ccaaggtatc tccaaaattt gtacaaatac aatacaagcc aaaagcaaga
     6601 taaaggataa agatggtaaa ccaaaaccaa tatcttctac gcgccttttc ttttcgagac
     6661 atatcttcca cctcccttat atagctctat tatattccta agtcttttat ttgtaaagtc
     6721 ctgacttaga gactctctaa aaaatctgcc aagtcaacca cttcttcttg ttcactcgtt
     6781 ttcatacttc caagaacgcc catcaagtcc tcccaactcg tatccatcac gtcgcgaata
     6841 ctcattccat aaggaccctc tgtaacttgc ttgataaagc cataaaaacg ttggatagct
     6901 tgcccaggtg ctagctcttt tcctttgggt ctacatcacc taccagatga gcataaatgt
     6961 ctgtaaaaat agaaatcact tttgcaaaat ctgtgtattc tagtaattgt tctactgtca
     7021 catcatcaaa caaactagca ataaatccca actgcttatc aagcttctct acctcagata
     7081 aatcacttgt caaggaatcg ttcatgacca aatagtcacg ataatcacga gttgtgattt
     7141 ccttactctt ttttaggaca tcttctccct tgtcattttt gattgtaaat tgaaccttag
     7201 ccatatactt tcctttctaa aaacggtaaa aaagagagct ttcgccctcc ttctaccctg
     7261 cagcagccat gtttagttga cccttcaatt tcttcaactt ttcagcatcc gaaccataga
     7321 caatagcacc ataacgaccc ttagttgcgg tgtctgaact agctgtcgca gcaaaattca
     7381 cattagtagt agctaactca ttagccttat ccttgattgt ttcaaaatca atagcatcca
     7441 tagaaagctg acctttataa aaaccatagt aaaccggttc cccatcagca gtattacttt
     7501 caatgagaat ggaaacattc ttagcaacag tgtccgcacc gaagtaatag ataccatcat
     7561 catcgccata ccctaaagca ttcgtatata aagctaatgg gacatcaagc aagcccatct
     7621 ctaccttaac atccccgact cctcgattgg agacgtgata agcaacatta ctaccataag
     7681 tctttacagg gtcactagat aatccagtta ctctagcggt ctgtgtagcc ccttcattat
     7741 ctttaccttc tagcgtaaag aggttctgtc caaccgttgg agtctgtcca tcatgcacac
     7801 gaatggtgac tgatttaagt ccgataagtg cagttccttt tactgtcatt tctttttcct
     7861 tctttctagt atttctcata tagagcgctc ataccacgat aagtccgaac gtctacatag
     7921 cgcttaatat caggaatcca ttgttccaaa ccaccttcag tctgataaaa tccctgttct
     7981 tccattattt tttcaattct tccttggagt tctttacact ccactcgatt agtagactct
     8041 acattgattt gatagagaaa agtcttagcc aaactagtat tactaccatg agccgtctgc
     8101 attggaggcc ctacagggat aatgacaata ctggtctcgt catctcccaa gctctcagga
     8161 cgctcaaaag acttgatact aataccagat aaagactcat cctcttccaa agcatcgtag
     8221 agttcagtta atttgtcttt aatcattaca aaaactcctg ttttaacttc atgccgactt
     8281 tagacttaaa gaccggctta ctcgcttcaa aaaagcggcg catgactccg aaaccacgag
     8341 gatgcccatt ctttgcatag ccaaattcat ttaagtgaat aagagtccaa cgaggacttt
     8401 taaaacctaa tttaaccatt ggaactccgc tggatgtacc agtcacattc ccatgaacga
     8461 ccgcaccaac cgtcttccca gtgtcagcgt acaccgccat agcccgtttg aaagttggct
     8521 caaactcctc aaccgtctcc ttcaaggctc tgttgacatt tctacgaact accggctctc
     8581 ctagtcgagc ctcaatattc ctcaaaatat catcaaatcc ttttagatta gctccactag
     8641 acatcacgac cacctccgat aaggacaatc aaaaaatccc gattatcata atcaggacgg
     8701 acatcaatga tttgccattt cttaccaact agacggatat cccccacttc gacaaaatgc
     8761 cgattttcag gctgatagtc tgtcagaggg tcacgaatct tcaaagtcat cttagctttc
     8821 atcgcttttc cagtcgcaat ctcaacgtct ttcatacttg gagaataagt ttgccccatc
     8881 gtataaaaag ccttctcgta actcacatca cgaccatcaa cccctttttt tattttagag
     8941 gtatagaaag tcaggggagt tctcaggtct ccattttgag actcaggctt tttgtaacga
     9001 tagcttggtc cctcactcct cttcatttac actcactact tccttagagc tacgacttct
     9061 ggacgacttt tccaactcga catagccagg taaatgttcc tttagctcag caaatcgttc
     9121 ttctgtcgct tcaaaccttc ctccgacctg cctaaggact ccctccttga ggtcataaaa
     9181 ctcttttaat accttaatca tcactatcct ccatttctat cttatctaac gataatgcta
     9241 aaatatccgc tttgaaattt tcgtaaaaaa attcaacttg gtcattatag acatagcgag
     9301 cacgttctaa gataagttcc cttatttgat gatcgtgtat attcccactt cctaccaagc
     9361 gggtaataac catttgagaa ctttctaaca tccgtgagag attcgcatct tctccgctat
     9421 gaaaaattct catccgctcc ttaaaaggat caaggaggga atgaagttta gcttcaatct
     9481 ccatccttag tcacctttta ttttccaata ttcagtgtcc acacagcagc agtcttttca
     9541 tcatgcgctt taccataagc aaattgctta gcagtgtaga gattcaagtc ttctaaagca
     9601 taggtttcgg taaatcgtcc aaactcaatc ccaccaccta caaaggcatc gtagcgacct
     9661 ttgacaaacg tcgtcacctt accagaagtt tgagcaacag actcaaccaa gatgaggtta
     9721 taaggcatag ctgttacata tacaccctga gcattgagag acgtatattg cttcttcaca
     9781 tcccaggcat ctacagggtt cactaccata accaaattac cttctactgc aacaggagtg
     9841 gtattgtcag cttttgtaga atggtattta taaacctccg tcaactcttt taccacggta
     9901 gcagagtctg cgaaagtcaa tggttttttc tgagcttgtt tctcagcata agttactttt
     9961 tcactttcta cagtccctgt aagagtacga gacagcccaa taggttgctt gtccccatca
    10021 ccattcaaat aaccagcttc aagagcagca gcaaaggcct ctgtaatttg aatagagaca
    10081 taagattgca accaagccgg accaaatttc tcagagtctt tagggattac aacaaaagca
    10141 gtcaatttag actgaatcgc ttcttcttca gcaaattctt gtttcaattg tccttgaatt
    10201 tcagcattga ttttacccca aaccgcttga cctgtacgac tagacttgag gaatttcaaa
    10261 cgaataccag cattacgcaa gccgatgtgt tgaagaagtg gacgggctgc caccatatcc
    10321 tcaaaaatac ggtcaattgt ttcctgaggg aacaatttct caattccttt gggaggaagt
    10381 ttctcaatgt tgttgaaaaa ttcgcgagct tcagcagtca actgtgcatc gtatggattc
    10441 atagttgcga tttctttacg agcagcttct ctagcatcat cttgcaactt atctgtcaat
    10501 gcctcaatca tctcgttata gagcttattt tgctcctcca taggagcacc agtttctact
    10561 gcattcataa agttctgacg agcagttgta aattcattac caagtttcat cattgttttt
    10621 tatttccttt cttaaaatgg gaaacgaccc aaccccacag gttcagccgt cttgtcttct
    10681 tttttcttat cttctgggat aagtaaactg ttattcaacc tatcacgaac caagtccgcc
    10741 aataactcca catctggggt catcgcagat cggatttttt cgataaaatc acgaggaatc
    10801 acaggtgtct gactagctac caagacagga gcttcctgac tttcaaacat gaccttatct
    10861 gcgaagccat gattaacagc agaacgagca tcaaaccacg tttcaccctc cattagatcc
    10921 agtaaatcat ctaatgcctt accagtctta tcaatatagg catatgcgat ggacttatta
    10981 aacccttcta acacaccagc ctcatgcaag agcgccttat ggtcaccacg tacaccagca
    11041 gatacattat ggatcatgat ttgcgctgta ggactaattt ccacctgatc accagccata
    11101 gcgataacac tcgcagcact agcagcaatc cccacgattt tcaccgtcac atgcccctga
    11161 taagcacgca atgctgtata gatttcacta ccagcataca catcaccacc accagaattg
    11221 atgtgcacct ccacatcttc tcccgtcgtc ggcaatacaa tatccttagg agcagttgca
    11281 tccatgtcta tccaatcata aaaccagcgg tcatcattgg atacaatcgc tcccttaatc
    11341 tgaatgattg tcatcctcat ttcctccttt ctctatttcg ttttcaccct gatagttctt
    11401 agtgataagg aaagaatccc caccaggaac agcctcaaga ccaagttcca atctcacctc
    11461 atttcgagtc atagccccag atgagatgag tttatctatg ttctcagcca gagcaaactt
    11521 atccagtccc ccctcaccaa aaatcacaaa ttttttctga ttagcgtatc cagccgaact
    11581 caccatagca tggtttagag cgtcacccac ttttttaacc agcgattcat aacaataact
    11641 gttaaacatc ttctggctat tagacaaatc agccatatct ccatgcatca aagccgtcgg
    11701 caaacctaat atatccgcaa cctcgtcatc aaattgacgt ctcagtttct tcaactcttc
    11761 aactgataga tttgtagttc ctaccgtatt ggtcaactcc gaatactcca caccatccat
    11821 tgtcggtaca atggcaaccg tcttagacgt aaaggactta aaaagattgt ccgcatatct
    11881 ctgcagtttt tttcgcttgt catcgtcaaa ggttccattc gcttttgtag ccagaacccc
    11941 acgaatttga ttatttctag ctaaggcttc cactagacga gtatgcagtt tttcataatc
    12001 agaaaacaag tctgatacat attcttgcaa gcgattgttg ttatactgga gaaagatgac
    12061 atcactcata gcaaacttcc tctgaaatat ataatccccg acagacacca tctcaaaggt
    12121 gtcatcatac agagcataac gcctacgagt aaaagcatca gctaccagta actgcttatc
    12181 atcagacaag acaataagta cttcgttttt cgtcaataag cgataaatga ccttctgcca
    12241 aaactctgag gccgactcat tcttattggg acgcacattt aaaatataat cccagtctga
    12301 cgccacatga tgattctcca ccatataccg aaattccgac ttagcaaaga tacgagccac
    12361 aaactccgcc gacttatcaa tcgctaaact cttcaattgc aagcgtccca acattcgttc
    12421 caactcttca aagtcaaaac caacatcagg cgcatcacgc ttaaataagt ttaagaatcc
    12481 catgcttccc ctccttcctc ttatttttgc cgaccaccca cccaaaattt attcttagat
    12541 taaaagtccc agctatcgag catgtcaagg aattccccaa cgtttgactc atgtactagc
    12601 tccctcttgt atagagcagc aatcaaggca tggaaaccat ctgtctttct tctgaccggc
    12661 tctttcttca agaaacgctt attgccatcc ttatcctctt tgatataggt attatccgta
    12721 taccaaatca tagagttatc gttctcaaag acaaaccgct cattggcaaa tccgtcttca
    12781 atgattggcg caaccttaga ttgaatcgcc ccaggattcc gcaagaactc atattcaaaa
    12841 ccagcctctt ccaaaagcgg ttttaacaag tccattctga aaccatcagc acatacaagt
    12901 tcaatctgat aaaacttact ccattcttcc agcttcgcaa ccaacagccg cggatcaatg
    12961 ctaggaccgt ccacaatcgt aaacaaacct ttatctgccc attcctgaat aggggctttt
    13021 agtttgaaag ctttcaaaaa cactttacga gcaaatgaat gttgtttcca gataaactca
    13081 tccccgttct taaacaacaa accaacactt gcaaagtctc gaatactagc atagtcaaac
    13141 cctgcaacac aagacttacc taacaagtca ataccaggtg aacgcaaaca agcaactaac
    13201 ttttcacgag aggtgacatc tttctcaagg tcagcttcag gaaggttcat ccgtttagtc
    13261 ataaactcct gacgaccaga tggttccaac tcaaggtcat catagtcagc tttagttctt
    13321 gcaagtaacc tcttagcgta aggagtgctt tcatccaaca tcggatttgc ctttggccac
    13381 ttcttcatat catccacttc atccgcactg tctaacttac agatgaaagg aaagagcctg
    13441 aaatcatcaa cctctccgtt caagatttgc atagacttct ctatcagctt gtcataaaat
    13501 ccctcacgca catacccatt cgtaccgttg tagaaagtcc gagcatgagc aatcttaccg
    13561 agacctgacc gttgaacctt cacggcctta tcatcttcaa actggtgaat ctcatcaaac
    13621 tcaagacagc catcacgagc ggagtccatg gtcttcggat tattcgtccg aaaagaaaag
    13681 accgagttgt tcgctcgacc tgtaatagac attttagtta gatagaaatg gtcctcaagg
    13741 ccacgccttt gaatagtctc atagacctcc tcaaatgaaa ccttgccctg tttctcagaa
    13801 ttggcagtga tagtcacatc ataatctctg atagggtaga taggactgat aaagaacgag
    13861 gatctggcag acataaaacc attcttacca cccccacgag caagtgtgta caaatactcg
    13921 tcgaagtgtg gctccccgtc ttccttccga aaaagaaaga taaacggagt caagaaaagc
    13981 tgatacttgg ccagagggaa aaaattcttt tccgcaaaac gaatgaactt atcaatcaag
    14041 tcattatcaa aatacaaatc atcacgagga tagattttct ccttgatgat tttaaacagc
    14101 aacttccttt ccttgttgac gacaatttct ccactctcgg ccattttgat gtagtcatca
    14161 accaagggat gagaaatcat agcaggtcac ttccagacgt gggtttctca acaggagaat
    14221 tttcaacctc aaaatcaaac gatcgctcaa tagccaaaag ctgattgctt gttgtgttga
    14281 tttccttgat gagagaattc gctttttgga atctctgttg cccattgtga acagtgatga
    14341 ccaatccgtc ttcatgaagt ttagctttca gctcatatag caatctgaca agataaagat
    14401 aacgattcac tttttcgtac tggatcgcat ccttttttct cggactgaaa tagccgattt
    14461 tggaaagtag ctgattttct aattctttta tatttttttc cgagtattct tccattaccc
    14521 cccaccccct ttaatttttt gttaaaaatt tggacagtcg agtgcagacc gcttactggc
    14581 tcctttgaaa tttcctgatt tttttgaccg gggggtgttt aagattcgtt cacctaaccc
    14641 caccattcat cttttcggaa atttctgtca ttcttatcaa aacgatcatg ccttttatta
    14701 tgacatgctt tgcacaatgt tcgtaggtta tcgatatcaa gcgcgaactc tggatataac
    14761 tctagctcct tgatatggtc aacttctaaa ttagtagtcg tgaccttgcc ttcatcccta
    14821 caccatacac attcgtaatg atctcgttta agtgcttgcc ttcttatcgt tctccactcg
    14881 ctggaattgt aaaactggtt gcgctcttct cgagttgaaa catcaatcat ttgattattg
    14941 atgttgatac tttgagctca aatttattta gcttgtcaat acaattgttc aagtgttcga
    15001 ttgcttcaca acattgttga gttaactctt ttaattctga gcgattttca atttcgactc
    15061 caactacaat ttttcctaat ggtttttgtt tagtggttct tttattaaaa agtcttttaa
    15121 taataccttt cataactgtg caatctcctt tgtttttact ctttcaattc cttgttttac
    15181 atattctagt gaactcgcta catgagtttt aactcagctt tatcaagcgt ttatcttgca
    15241 tgtgcgaaat gaaatcatca taaccttaaa acaatgaatt gatgttaaaa taaaaaaatt
    15301 aaaagccctg aaatttcgtc atggctctgt cttgtgaatc ttgatttttg ccgatatacc
    15361 gaagtgaaat actctggctt gagtgattca gtaggtccat tatcagagcg acatccttgg
    15421 tttgttcgta catgaataaa ccaaaggtct ttctcataga gtgagtagct atgttttcca
    15481 gaccaacttc ttcagcagct cttttgataa tcttataagc tgtgttaggt tttatatgct
    15541 gatgctttcc gtttcggctt ggaaagagga agtcttcatc tttcttatct tttatgtact
    15601 gcctcatagc attcttgaat ttctttggca tctttcgttt ggttggcttg tctgtctttt
    15661 catcgacgat ctggacatgc caacctttaa cgtgctttac tttcagttta acgatatcac
    15721 caatacgaaa tcccaaatta acaccagaaa ggaagagcat gaggttgcgt tgtctatctg
    15781 actctttgac tgcactatgc aacgtcagcc attcaatcat aagctgaaca tcatctctat
    15841 ttcggattgg ttcaacaact accacatatc ctcacctcct tttaatgcaa aaaaaagcag
    15901 aggtttcctc tctgctattc ttcatgatac taatttacca cattaaaatt atcatttgct
    15961 atcattctta tcatacattt tagataattt tagtaacgct ttatgttttg ctcgttggat
    16021 agtggcaggg ctacaattta gttttatttg aacttctttc caagataatc cgtcaatgta
    16081 caataatcgc atcacgatgt tttccactgg atcatcgagc gactcaatcg cttgaacgag
    16141 ttcgtcacgt tcgtggtaca tttgtttgat ttctttatat agctgttctg acttgtcaat
    16201 aatcaacaca ttcaattctt cagtttgatt cttgttactt tttgattttg gcatgctgtc
    16261 gaactgctgt cctcgtaaaa tacctgactt caaactgatg atttcttggt gctttgactt
    16321 agctttgata tcgatataag gcaaagcctt caatcttttt ttgatgtcta ttgccaaatc
    16381 ttacctcctg accgggtttt cgtagatgtt gccgatgatt tcctcatatt ccgtccacgc
    16441 atatccatct ccaaatcctt ttaggtatac agcaggcatt ccacctatga atgtaccacc
    16501 atattctttt tctaaatata cttcatggag acatcctctt gtacatttaa cgatgtcacc
    16561 gatgaatacc tccttgccgt tcttgtctct gagtcctgtt gattgtccta atgttgctgg
    16621 atttacagga caccaagaac ctatagtaat gtattgttca ttggcttcta ccacttcgtt
    16681 gataataaat gctcttcctc tatcttcaat taaatgtccg tattgccatt ctcctttgct
    16741 tttttcgtca atggataacc ctctaaattt tggtatcatg ctaacactcc ttaaataaac
    16801 aaactagcta accatatcaa aaatgcacat gtaatgattt ttgaaatact gctctttacc
    16861 gcatacgaat aatcctcttc agattctttt ttgctagata acacaggcca gatgaaagat
    16921 agtagtgcat ccatccctaa tgcttgccag actgtaattt taccaactgg aacaattgtt
    16981 gtgataatct cattccatcc atactgaact acaaatggcg atacaacgat tacaaatacc
    17041 gccccaataa tgattcctag tcttttcatt ttataaatcc tcctctttga cgaaagcacc
    17101 atcaatccaa cgaccctacc gttttctaaa tcacggtcaa taaaccattg tttgactttc
    17161 tctattgtgt tcatgataac tcctttgcta ttgctgctat gacattaact gtcacgctat
    17221 ttcctgcttg tttatataat tgactgttag agttgacctc ctgcgcctta tcaaaagccc
    17281 aatcaggaaa accttgtaac ctccagcatt ctctaggtgt tagctttctg attcgaaagc
    17341 catctgataa atgattgttt tcgtgatagc tattgctagt taaagtagga gcgatgtcat
    17401 gttctccacc tttattataa ccatgcccac gttgaataat tttaggctca agtcctccac
    17461 cttgataggc tctgatagtt ggtgcgatgc cgtctgtttc gtaaaccacc ccacattgat
    17521 taaaattggg ttgcaatacc ccaaattgtt ttatagtatt gctttttata gctatctttt
    17581 gcccctctcc cttgtttgtt gttagcgtgg gagctagacc atcagcttga tagacttccc
    17641 cattcattcc gttgccagat gggtttatat tcccaatttt catgactgat tggttactag
    17701 ttgactgatt ttttccgctg agaggaaaaa atcttctggt acgttctctt ctaagatgtc
    17761 cgataatgaa cactcgttcc cgattttggg ggactccgaa attcttgctg ttaagcactt
    17821 gccattccac gttgtacccc agttcatcca aggttgagat aatggtctcg aatgtaactc
    17881 cattttcgtg attgagcaat cctttaacat tctcaaggaa tagatattta ggtctgagaa
    17941 tagatgcgaa cctagcaatt tcaaagaaca aagttcctcg tgtatcttca aaacctcgtc
    18001 tgtttcctgc aattgagaaa gcttggcacg gaaatcctcc acagataatg tccacacttc
    18061 cgattcctcg aatagattca tctgatactg ctgtgatgtc atgtaattct atttctcctt
    18121 tcgtattgtg tatcgcttta tagctttctc tagcaaactt gtcaatttca caaaagccaa
    18181 tacatttatg gccggcagac tccattccta aacgaaaacc gccaattcct gcgaataaat
    18241 ccaagaattt cacaacatca cctcatcccc aactttcact ttctcataca cgttcttcaa
    18301 aataactatg aaatttactt aaattgacaa tagcgacctc ttcaacagaa tgctttccga
    18361 tgtcaaagtc tggatcattc ttcccaaact ctttttctat agctttttca gccagaaaag
    18421 gcaaatcgaa tatacttgcc ccatttctta aagcgagcgc ttgaccgtat ttgttcacta
    18481 ttcgataccc tatatcaaac ggtctgattt cccttgggac ttttatgcat ttacttttat
    18541 tcttcattcc ttcttcaagt gtttgtgtca tcactccacc tcctcaatct caatacctgg
    18601 gcaatcaaac acccagccga cccctacttc ttctagttgt ttgcgagtaa attttgtagc
    18661 tagtccaccc atagagaaga atagtttctt atccatagca ttataatata gcggttgttt
    18721 tgttgctttc gtcactactg tataccgctt ctcttcctcg acctcgtagc cgaactggtg
    18781 catgttgacg agggtttgaa taggattttc tctgctatta ttaatccaat tattaaactc
    18841 tctattttct gattttttag cctcatccct gaaagcaatc caatcccaaa cattaaattc
    18901 aaaagaatcc ttattctctt cataccaatc cgccacaaac tgcggtacta cgactttaat
    18961 tttggattta tcaacgatgt cgtctgtaat ataaatatta ttgttgggta cattgagagt
    19021 ttgcccgtat tccaatctaa caacattttc aacgcacttg ttctcatcca catcaaaacc
    19081 gactattttc cctttcaaga gaacttcatc ccctataaaa aagttaaagc gtggtgtttc
    19141 aattagatct ttattcattt tccacctcct ctatatcaat tccttcacaa tcaaacaccc
    19201 agccgaagtt tgcgtcttct agttgtttac gggtgtggga atatataaca tcgtctaaac
    19261 taaagccttt tgtaaagaaa taccttttca aaaattctcc ataaaccaac atattttctt
    19321 taatattccc tttaatctta acaaaatacc gcttctcttt ttcgacctcg tagccgttaa
    19381 gccaagctag tgcgaagagt tcggaattat cccaatacca ttctgcaact atgtcagaca
    19441 tgcttgcgtc tattgagtag gacagtgtat gacccagttt tttctgttct gcgataaaat
    19501 ccgccacaaa ctgcggtact ttgactggtt gcggttcgcc tagttgttca aagatttctt
    19561 ttacatcctt ccaccaaact acataacctt gaaaattcct agtcattgtt cttcgttcct
    19621 ctaatttttt aatcaattcc tgcttattca tcttagtttc ctccataaat caaataaact
    19681 gcaataacta cctgagccat gcttggcgaa tagccaatcc aatcatcaaa ctccctagat
    19741 tttggcaacc aatccttagt agctcccaaa tcatagtctg taggcttttc atcagcaaag
    19801 atgcattcca tcgctcccat aaacgtcata ccatcttctg ccatttccca aaaatagtcc
    19861 gcccggtctt tcaccacttg tggtaaatct tgcttgggag gttgcggctt cccgtcttct
    19921 accgtccagt tgtatactgc attaactttt tgctttaact cttccatcat cttccaactc
    19981 ctccaatttc cgtctcaatt ctttattctt tttcctcaac aaatcgcgct ccagcgctct
    20041 aatccgtctc ttgcgtgcat cgcacggctt cgaatactcg attatcttct cttcgttttg
    20101 ctcgatcgtg cgtttcagtc cgtcaatctc agcctgttta tcgtacttca tcttctaaaa
    20161 atctttcaat agcttctctg taggagactt ccaccagacc gtctaagtcg ttcagggctt
    20221 caatatagtc tggactacct tgcccgtact gctctttcaa aaattcaaca aagagatgaa
    20281 tttcctgata ggttactcca accatgtttc ttacctccca ctaaaaatgt aacgttattg
    20341 atcatctttc ttttcctttc ttgctacacg ttcctcgact aaatagccga gaaataacca
    20401 cagaatagcc attccaaatt ctttaataag ttcaatcatt tttttctcct cctgaaaaag
    20461 tcgctaaata gtaacaatcc ttagagccgt agtcaaatcg tgtcgtccgc tgaccaatgt
    20521 gcttctgaaa tcttggatga gtgatagccg agaaagccca ctgatgatct tccatctgtt
    20581 caatgagatc atcgacattg tcaaacgttc caaggtaaaa cttgcagtgc ccgttgtaga
    20641 cgaagtaaag ctctaacatc actccacctc aacgggataa aaattcccaa aggaacccct
    20701 caatgcctta ccaacctgta cggctgcccc acgagaaaca aaccgcatgg ctttcttctc
    20761 ctcagaacat gaaatgtcca agccagtcac accgataaca gcggacatca gaaacggctt
    20821 atcctctctt gtcccatgct ttaaaataaa catcagccac ctccattcta aaaatattgt
    20881 ttccgcttgt tcgtcaaatc attgaaaacc atcaaatggt ctttatccac ccccttcatc
    20941 agtctggaca taaagggtct gccatatctt ttctgaatat cagcagaaat caaattcgtg
    21001 gtaatgattg tatttgaacg cttattcagg atattgtaga gaatagtaaa cgaccattcg
    21061 ctatccttct ccatgcccaa atcatccaaa accaagaact tagcgctagc aatcttattg
    21121 accagaaact cttcctgact aaaatcagct ttaatcttca tcagcaagtc cgtcacattg
    21181 ataaatatag caatctcttt cgtgtactca gataaagcct ttaccatagc aaaggccaaa
    21241 tggctcttgc cagttccagc ttctccttgt agcacgatgt tgttcctagc accctcagac
    21301 cactcacgac aaatcctctt tgcaaaaact agcttttccg cttctttttc ggttggcgtc
    21361 tcaaaattgt ccaaagtcgc atttttcaaa acctcatcat aaagagagaa cttttcaaga
    21421 tagtatttcc tctctcgctc attctcagcg tcggccagtt cattcactct tgcttgattc
    21481 tcctcatgga tccgctcaga ttcacacatg cgacatacaa cactttcagt ccgcaatatc
    21541 tttatcaaag ggatgttatg cttttcacag aactcttctt gttgttctgt attcctacga
    21601 taagataagg cgatttcctc aaacacattg tctaccatac tagtcgacct ccgcattcat
    21661 gccagctagc catttcagac aagcaggcaa ccacttgatg aattggttgg tctgctaaaa
    21721 gagttttctt ctcgtagctt aacggataat agtcaatctc gaattgttca attagttcta
    21781 gtacccccat tcgtccttgg cctcctgttc ttctttctta tccttgttct tcttttccga
    21841 ttgacgaacc tgttcaacag tggtaacatt gttcatctgc caatttctta aaatcccacc
    21901 aatatatttg atgttcggct ttcctgagtt aatagcagtc ttcaatgctt ctttcaccaa
    21961 atccacatca ttctcattta gtagatggtt gatttcttca atttcaaatc cagataagag
    22021 tctacgaaac tcagattgaa aaagttctaa gatattttct tcactaccac tactagtagt
    22081 agttattctt ttcttattct tatctttatc taatctattc ttatctttat cttcttctag
    22141 tgcgttaccg tccgttactg taacgttacc tgtaacgtta ccaagagcaa gatttttttg
    22201 ttttttacgg tatttggcta cacggttacg tgtctgttcc ttgattttct ccattccgtc
    22261 aacgttttga tgtttttccc aatttggcaa gctaataata ccatcgataa tctcaatcat
    22321 cccaaactgt tcaaaaactc caatagccat tcttactgta ttcaatggtc tacgaaaaat
    22381 agtagctaac atttcatctg tatagtgaac cttatcagtc atcatcaaca aaccattact
    22441 gttatgtttt ccagcaagtg tcaaaatctt gaaccatatc actaagatgg catcaggatc
    22501 aggcaaggca tcaatcaggc aaatcttttc atcgtcaaaa atatcggttg tgatttttat
    22561 ccacttaatt tcagacatac cgagcacccc acttcctgcg attggcacga tacttcatac
    22621 gcatatcctc atagatgtgc ctgccttcca gctccatttt ttcaatcttt agcagcttat
    22681 ttttaagctc cacatcacga tagtccttag ctagtttttc atagtctgtt aggtattctt
    22741 tgactcgtaa tagattttta taatcgtttt cccatatcgt aataaaatgt cttgaagttg
    22801 attcccttcc ttccagttct ttaacaatca taatcaggtt atccagcgat tcaatcaatt
    22861 cttccatttc ctgacctcct cattacaaaa atctgattcc agactgttta ggttctggca
    22921 aagctaacag ctcagggcgc aatcctacag gcggttcgtt gtcatatgtg aatcctttga
    22981 actctctgcg aatattcttg cgaatttctt gccatttgtc ctctctacca cgttcatatg
    23041 catgattacg cacttggata atcatatacg caaattcttg ctcttctcgt ctttcttctt
    23101 ccttgcgttg ttcctgcaat ttgatatgac ggcaagcccc tgcaaatcca agcagcaagg
    23161 ctccaacccc catcagctgg tctaaaatcg gtggttcaaa catttttatc tccttatcct
    23221 ctttttgtgc tataatatag tcaaataatt ttgctaagac cttgtccaga agccttttag
    23281 taaagttatt atatttgatt agagagccat tctttgatgg ctctttttga ccatttctta
    23341 ccaggtaatt cctttggaaa tccctttaag taacgataat tatctgaaaa tgtggcatac
    23401 ttaattccta gaaaatcaca ggtagtgttc acatccatca gctctggata gtggtcacta
    23461 tctttttcta tttcgactag ccttgtgatt gtgtccttga taatggattt aatccattca
    23521 gacagtgaaa gtagaacatt gtccatcttc ttcccctcct acccttcgtc aaatgagttc
    23581 aattccatga ttttcatctt ggtattggtg cttggctccc aagtcatcca ataggccaag
    23641 gctgcatctg cgaatttttt cggtagcaag tcatagcgat taatgttgaa gtggtcttta
    23701 aagtcaatct cagcttgtct aaagactgac tgagcgaaag tcttatccgc ataagccgga
    23761 ctatcaatgc cacctaagca agccacgacc cgagccttac gcttcttcag taatgattga
    23821 gcatagctag gatgaatcgg ttgctcactc ttgaggtagt caatatcttc aagcatggta
    23881 acctgttgct cacgcaattt cttttgacca gtgaatagag cgataaaggc atcctcgtcc
    23941 aagtcctcgc gaatgaaacc gccctgcttg cgaatagctg gcaaaacctc tgatgtcacc
    24001 cagcgcttaa actctttagc ctgaggcaac ttgctggata agataagaga gtagagacca
    24061 gattcgttga tgataatagt ttcttggacc cttcctaaat tatctgtgag gccctgtttt
    24121 agggcgtcat cttcatcaac atgaagagca atcgcatttc tagccttgct atatcctagg
    24181 atgtctgcaa catctttccc aacgaaccaa ggctcgtcat caattgtcaa agtacggact
    24241 tcctgcccgt gaaaattaaa aatttcgttc ataatattcc tcttcttact tttcctagtg
    24301 ttaaaatagt ttcccaaaca tctagtccct caagactatc gatcatcatc tgactaagtt
    24361 ggtgattttt cttctgccaa ttcagtatta ttttcgcttg catgtatgga cctctcagtg
    24421 atttctccaa gggttttcaa tacccaaaac atctgcaact ttttccttaa cagactcact
    24481 acctttgcca tacttcagca actctgaaat aactgatgat gctacagata cttgttttgc
    24541 caattcagct tgagtcatat ccaactcaat caaacgagtt ttgattttag ccttgattat
    24601 ctttagttct ttactcatct ttctcctttc tatttcttcg tttcactttc cagcgctctg
    24661 agttctatct catggctggc ttgtttaaat agcttctcac acgctatttt agcttctctg
    24721 tacgttgtgt tctcgctgat gaagtaatca gcaagttcga tgattttatc ttccattcaa
    24781 cctcctatat cagtctcaag actgaggtaa tatcttccta aattgctata ataatcttga
    24841 ctaggacctc tcaccgtttt agtcaaaatt ccaatagaaa ggaggaaatt ttatatgtca
    24901 aaactcacta aagaagatgt tttacaagtt tctcaagaaa ttatcaacga tgctattcca
    24961 gttatccaag atatgttaga tgaggtattt aaagaatacc caatcgacat ggaaattaga
    25021 aaggctattc ttaatagcgt ccttgtcgct cataaactca gtacagaaac tacggtttcg
    25081 ttgctaacag aacttgtaaa cgctcaagaa aactagtgtt tcttagaatt ttttctacta
    25141 attcagggtc tgcctttacg agggtagact cttttttccc actatacgga tatcgtcttg
    25201 gtctcatttc ctcacctcct tttaaaaaat tatctaaaaa gttagcgaat ttcttgacat
    25261 tgataaataa atttattaaa atcaaaacat agagaaaaga cctactaaaa agtaaggtta
    25321 tacctagaaa acggacgcca atcagtttca ttaggcttta ttttttagtt gtcttgttcg
    25381 ctaactcttt agcttacaaa aactattgta gtaaatttat taaactttgt caacagtttt
    25441 gtagtaaatt tattaaatat tttttgtcat gccttagaaa ggttgatgta tcaatgtttt
    25501 tcacatttga aaaaataaaa gaattggctg acaaacaagg tatttcatta aataaacttg
    25561 aagaaaaact aggttttagc agaaatacaa tttataacat gaagaaatcc acaccaaatg
    25621 ttgaacgagt ttcaatgatt gctgactact tcaacgtgtc cacagattat ttacttggtc
    25681 gcacggataa cccaacaatc gctggtagtc atgactacaa atgggaaggt aagactctaa
    25741 acgttgaaga aatggcatct aatgtcatga tgtttggcgg tcgagaatta acagatgaaa
    25801 agaagaaaat catccagtct atcattgaag gttatctcaa agaagctggt gattagaggt
    25861 actgcttagt gactgaaaaa gaaattataa gtcattttca gattcgtatt atcgattttg
    25921 atggagattt gatgcctgat gaacttggat tttacgaaaa agaaaccaat acagctttct
    25981 tgtctaacaa actcagcaag aaagagagag ttaaagtact actgcatgaa cttggacaca
    26041 aagaccacac acgctcagag taccagaacg ctcgcctacg ctgtgaaaac gaagctgata
    26101 ggaatatgat ccatcatctc gtaaaagacg cgatagaaag cttagacgac cccacagagt
    26161 ttgattacct caaattcatg tcctactaca atctaaaaac catgacgaat gaagtcatgg
    26221 taaaagagga atactttgca ttgatggagt gaaaggagac tcatatgtct tactcgtatg
    26281 ttgctttaga tgttgaaact gcgaatgact ttcgcggtag tgtttgttct atcggattag
    26341 taaaatttaa agatggaaat attgttgatt ctttttacac tttaatcaat ccagaagaag
    26401 agtttgatga tttcaatatt ttcatccatg gcattacacc tgaagatgtt cttgattcac
    26461 ctacattccc agaggtgaga aaggcgattg ttgattttat tggttctgat atagttgtag
    26521 cccactttgc acagtttgat atgggtgctc ttaaagatgt ataccaaaaa tacgagctgg
    26581 attttgataa tatagaatat atttgttcgt atcgattagc caaggtcgct ctccctggac
    26641 aattgaatta caaactaaaa agactagcta aaaatttgaa tattgagcta gaccaccaca
    26701 acgctttatc agatgcacga gcaagcggat tgattttaga atacttatta tctactaatt
    26761 cattttccga cctcaacgct tttttaaaag aatatagcta caataaaact ggcttacttg
    26821 gtcagtatgg atttaaaagg aaaaaaagtt atcagtacaa agaaaatctt atctatcagc
    26881 caacagaaga agaaaaggca gcaatgaacc cagaccatta cttttacggt ttgtactttt
    26941 gctttactgg aaaactcgag cgaatgacta gaaaagaagc taacaaagct actgcattag
    27001 ttggtggtat tcctgaaaaa ggagtgacta aacacactaa tatcttagtt gtaggagaac
    27061 aagattggag agtagtcggt acagatgggt taagtagtaa aatgaaaaaa gcacaaacct
    27121 tgttagaaaa aggtcaagat attgaaatca tgacagaaaa tgattttata agattgcttg
    27181 aggagtaatt aacaaaaaag ctccacaatc tccctcgcca aagtttgatt gtgaagccca
    27241 cccttataaa aaatcagcca ttaaaaaggc ctcttttcta tactctattt tacaccatga
    27301 aaggggtgat gtcaatattc tcaatgttta gaccttgtcc agaagctgat aaacaaggag
    27361 aatacaatga aatataataa aacaaaatac ccaaatatct attactatga gactgctaaa
    27421 ggcaagcgtt actatgtcag acgttctttt ttcttccgag gtaaaaaaag agaaaaaagt
    27481 aaaagtggtt tcacaactct ccctcaagct cgcgcagcct tggtagagct tgagcaacaa
    27541 attcaagaac aagaattagg tatcaatacg aatctaacgc ttgatcagta ttgggatatc
    27601 tattctgaaa agagattgtc aacagggcgc tggaatgaca cttcctacta cctcaatgac
    27661 aatctctata agaaccatat caaggcaaag tttggttcta tcctgcttaa aaatttggat
    27721 agaaatgagt atgaactatt tatcgctgaa aagttgcaga accataccag atacactgtt
    27781 caaaccctca attccagctt catggcattg ctgaatgatg ccgtcaaaaa tggaaatctg
    27841 ctctcaaatc gcttgaaagg tgttttcatc ggccagagtg atatccctgc tgcaaacaag
    27901 aaagtgactc tcaaagagtt taagacttgg atagcaaagg cagaagagat tatgccaaaa
    27961 caattctacg ctctgaccta tctgacaatt tttggattga gaagaggaga agtctttgga
    28021 ttgcgtccaa tggacatcac tcagaacgac agcggacggg ctatactgca tcttagagac
    28081 agtcgaagca accagacctt aaaagggaaa ggagggctta aaacgaagga ttcagagcga
    28141 tatgtctgcc ttgatgatat cggaacagac ctgatctatt atctgatagc tgaagcttct
    28201 aagattaagc gaaagttagg aattatcaag gaacagcaca aggattatat aactatcaac
    28261 gagaaaggtg gtctcatcaa tccaaaccag ctaaatagaa acttcaatct agtgaatgaa
    28321 gcaacaggat tgcatgtaac acctcacatg atgcgccact tcttcacgac tcaaagcatt
    28381 attgcagggg ttccgcttga acaattaagc caggcgctgg gccatacaaa agtctatatg
    28441 accgatcgtt acaatcaagt agaggacgaa cttgctgaag cgacaacaga cctatttctt
    28501 agtcatattc gctaaaaaag tccccgccaa ttccccgacc aaaatccgaa aaataccgaa
    28561 aaatatcgaa aaattatttt tagaatagtc cccaaaagcc tgaaataaag ctaaaaaact
    28621 ccacctgatt gggtggagtt
//
LOCUS       DAVHEX010000022        28017 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVHEX010000022 DAVHEX010000000
VERSION     DAVHEX010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28017
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.022"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            353..1516
                     /locus_tag="VVL45_000968"
     CDS             353..1516
                     /locus_tag="VVL45_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEU4187160.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFISKDKVAFNQAFFETLFRKAKEKRSAYYQDDLTTTYRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGLDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKNLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            1513..2190
                     /locus_tag="VVL45_000969"
     CDS             1513..2190
                     /locus_tag="VVL45_000969"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEU4187161.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQLYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            2180..3034
                     /locus_tag="VVL45_000970"
     CDS             2180..3034
                     /locus_tag="VVL45_000970"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEU4187162.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLVETVANIRRYQMFGINLSMPYKEQVIPYLDKLSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMILLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVVDIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            3053..4120
                     /locus_tag="VVL45_000971"
     CDS             3053..4120
                     /locus_tag="VVL45_000971"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEU4187163.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     IAEEKGLMPAGITQSITEMCQKFGLPVDYENWEVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSATIHPVSLEEMKDYLVK"
     gene            4130..5296
                     /gene="aroC"
                     /locus_tag="VVL45_000972"
     CDS             4130..5296
                     /gene="aroC"
                     /locus_tag="VVL45_000972"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEU4187164.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            5306..6409
                     /locus_tag="VVL45_000973"
     CDS             5306..6409
                     /locus_tag="VVL45_000973"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEU4187165.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLEAIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVIK"
     gene            6420..6758
                     /locus_tag="VVL45_000974"
     CDS             6420..6758
                     /locus_tag="VVL45_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEU4187166.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            6850..8133
                     /gene="aroA"
                     /locus_tag="VVL45_000975"
     CDS             6850..8133
                     /gene="aroA"
                     /locus_tag="VVL45_000975"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEU4187167.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKECTRNHTEDMLKQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLINPNSHLVLQNVGINETRTGIIDVIRAMGGKL
                     EVTEIDPVAKSSTLTVESSDLKGTEIGGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            8126..8602
                     /locus_tag="VVL45_000976"
     CDS             8126..8602
                     /locus_tag="VVL45_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEU4187168.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIENRLGMSIANFFA
                     EKGETAFRQVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLRADFETLY
                     QRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            8599..9447
                     /gene="pheA"
                     /locus_tag="VVL45_000977"
     CDS             8599..9447
                     /gene="pheA"
                     /locus_tag="VVL45_000977"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU4187169.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     NYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAVAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESCPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            9444..10718
                     /gene="psr"
                     /locus_tag="VVL45_000978"
     CDS             9444..10718
                     /gene="psr"
                     /locus_tag="VVL45_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HEU4187170.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            10997..11842
                     /locus_tag="VVL45_000979"
     CDS             10997..11842
                     /locus_tag="VVL45_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU4187171.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKDFVDRNLFITIRDKKT
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGDL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            11832..12935
                     /locus_tag="VVL45_000980"
     CDS             11832..12935
                     /locus_tag="VVL45_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4187172.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLTRRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYTSKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            12937..13923
                     /locus_tag="VVL45_000981"
     CDS             12937..13923
                     /locus_tag="VVL45_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU4187173.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            13939..15372
                     /locus_tag="VVL45_000982"
     CDS             13939..15372
                     /locus_tag="VVL45_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HEU4187174.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMI
                     SILGLTTGGILARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            15401..16435
                     /locus_tag="VVL45_000983"
     CDS             15401..16435
                     /locus_tag="VVL45_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU4187175.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPKESAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANNLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            16635..17372
                     /gene="mecA"
                     /locus_tag="VVL45_000984"
     CDS             16635..17372
                     /gene="mecA"
                     /locus_tag="VVL45_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEU4187176.1"
                     /translation="MKMKQISDTTLKITISLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEVKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            17523..18809
                     /locus_tag="VVL45_000985"
     CDS             17523..18809
                     /locus_tag="VVL45_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEU4187177.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILALDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            18811..19680
                     /locus_tag="VVL45_000986"
     CDS             18811..19680
                     /locus_tag="VVL45_000986"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEU4187178.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            19756..19827
                     /locus_tag="VVL45_000987"
                     /pseudo
     CDS             19756..19827
                     /locus_tag="VVL45_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            19975..20913
                     /gene="msrB"
                     /locus_tag="VVL45_000988"
     CDS             19975..20913
                     /gene="msrB"
                     /locus_tag="VVL45_000988"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU4187179.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLI
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            20971..22695
                     /locus_tag="VVL45_000989"
     CDS             20971..22695
                     /locus_tag="VVL45_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4187180.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFWSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFAESPENIHSELEQKQVTSGRVLQV
                     QELTFTYPDAAQPSLRYISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPIDKGSIDL
                     YRDGSSPRNLEQWRSWIAYVPQKVELFKGTIRSNLTLGLNQEVTDQELWQALEIAQAK
                     DFVSDKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENFPNMSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            22697..24445
                     /locus_tag="VVL45_000990"
     CDS             22697..24445
                     /locus_tag="VVL45_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4187181.1"
                     /translation="MKRQTVNQTLKRLAVDLANHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFADRQGNGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKIAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRASLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            24747..26006
                     /locus_tag="VVL45_000991"
     CDS             24747..26006
                     /locus_tag="VVL45_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HEU4187182.1"
                     /translation="MKVFQHVNIVTCDQDFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    26082..26217
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            26256..26756
                     /locus_tag="VVL45_000992"
     CDS             26256..26756
                     /locus_tag="VVL45_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEU4187183.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            26832..27200
                     /gene="rplL"
                     /locus_tag="VVL45_000993"
     CDS             26832..27200
                     /gene="rplL"
                     /locus_tag="VVL45_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEU4187184.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            27387..27974
                     /locus_tag="VVL45_000994"
     CDS             27387..27974
                     /locus_tag="VVL45_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU4187185.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQVLYGASLFS
                     FLWMYGQRKQVVKVNMKTRMKCLGIGLASLLIISLCFSLIHAQGSTNQTNLIGLQHQV
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALVHHPGTILAWCLYV
                     SLGMFLGMVRYKSDLWGSMGLHLVWNFLVYSLLLF"
BASE COUNT         8270 a         5204 c         6300 g         8243 t
ORIGIN      
        1 aaaaggcttc tctaaaaatc aaattttcca gatacctact gaagctgatt tatttcttca
       61 tctttcttct actacctttg tcactaaatt tgacagacac atattgctga tacaacctct
      121 ctacctataa tctcacagtt aacctgaggc tagtttcata gtttgctatt tgattttcgt
      181 tgataataaa aaatgctcct ccaagacgga agagcatttt ttattgtgat tcttacacat
      241 ttacttccta gttttgcagg aagtctagta taccattttt taaaagaaga gtaaaaaagc
      301 aagggtagtt tcttttttga gaattgtcta aaagtttgat ataatggtag ttatgaatag
      361 aataagagtc agcaaaaggg ttgaaaagaa gcttgctaag gggctagttt tactagaagc
      421 cagtgatctt gagaatgtca atcttaagga tcaggaagta gaggtgcagg gtcaggaagg
      481 aaactttctt gggactgcct acctttctca gcaaaacaag ggcttgggct ggtttatcag
      541 caaagacaag gtggccttca atcaagcttt ctttgaaacg ttgtttagaa aagccaaaga
      601 aaagagaagc gcctactatc aagatgattt aacaactacc tatcgtctct ttaatcaaga
      661 gggagatggc tttgggggtc tgacagtgga cctttatggc gactacgccg tcttttcttg
      721 gtataactct tatgtttatc agattcgtca gactatatca gaagccttta gacaggtttt
      781 ccctgaggtt ttaggggctt atgagaaaat ccgctttaag ggtttggact atgaatctgc
      841 ccatgtttat ggtcaagaag cacctgactt tttcactgtt ttagaaaatg gtgtcctgta
      901 tcaagtcttt atgaatgatg gcttgatgac aggaattttc ctagaccagc atgaggttcg
      961 cggtagttta gttgacggct tggctatggg taaatccttg ctcaatatgt tttcctacac
     1021 agcggctttt tcagtagctg cggccatggg aggagctagc cagacaactt ctgttgatct
     1081 agccaaacgt tcacgagaat tgtctcaagc gcattttcag gcaaatgggc tcagcacaga
     1141 cgagcatcgt tttatagtca tggatgtctt tgagtatttc aaatatgcca aacgcaaaaa
     1201 cttgacctac gatgtgattg tcctagatcc gcctagcttt gctcggaata aaaaacaaac
     1261 tttctctgtg gccaaggatt atcacaagtt gatttcccag agtcttgaga ttttaaatcc
     1321 gggagggatt atcattgcca gtaccaatgc tgccaatgtt tcccgtcaga aatttacaga
     1381 acaaattgat aaaggctttg caggaagaag ttaccagatt ttaaacaaat atggtcttcc
     1441 agcagatttt gcctataata aaaaagatga aagtagtaat tacctcaagg tgattagtat
     1501 gaaggttagt aaatgaaatt aatcgtttca gtaatgccaa gaagtttaga ggaggctcag
     1561 gctctggatg ccacgaggta cctggatgcc gacatcattg aatggcgtgc cgactatctg
     1621 cctaaagaag cgattttgca ggtagctcca gccatttttg aaaaattcgc aggccgtgag
     1681 ttggttttca cgctacgaac tcgctccgaa ggtggagaaa tcgacctttc tccagaagag
     1741 tatatccatc taatcaagga agttgcgcaa ctctatcaac cagactatat tgattttgag
     1801 tactatagct acaaggatgt ttttgaggaa atgctggact tcccaaatct tgttttgagt
     1861 taccataatt tccaagaaac acctgagaac atgatggaaa tcttgtcaga gttgacgatt
     1921 ctaaatccaa aacttgttaa ggttgcggtg atggctcaca cggagcagga tgtcttagac
     1981 ttgatgaact atacacgagg ctttaaaacc ctcaatcctg aacaggaata tgtgaccatt
     2041 tctatgggta aggtgggcaa ggtctctcgt atcactgcgg atgtgactgg ttcgagttgg
     2101 tcttttgcca gtctggatga ggttagtgcc cctgggcaga tttctctagc tagcatgaaa
     2161 aaaatcaggg agattttgga tgaagcttga tggctataca cgtttagctg cagttgttgc
     2221 caatcctatt aagcattcta tttctccctt catccacaat agagcctttg aggcgacagc
     2281 taccaacggt gcttatgtgg cttgggagat tgaagcgagt gacttggtag aaacagtggc
     2341 caatattcgt cgctaccaga tgtttggcat caatctgtcc atgccctata aggagcaggt
     2401 gattccttat ttggataagc tgagcgatga agcgcgcttg attggtgcgg ttaatacggt
     2461 tgtcaatgag aatggcaatt taattggata taatacagat ggcaagggat tttttaagtg
     2521 cttgccttct tttacaattt caggtaaaaa gatgatcctg ctgggtgcag gtggtgcggc
     2581 taaatcaatc ttggcacagg ctattttgga tggcgtcagt cagatttcgg tctttgttcg
     2641 ttccgtttct atggaaaaaa caagacctta cctagacaag ttacaggagc agacaggctt
     2701 taaagtggat ttgtgtgctt tagaatatgt ttctgaactg caagcaagga ttgccgagtc
     2761 ggatttgtta gttaatgcca ccagtgtggg catggatggc caatcctctc ctgttcctga
     2821 aaacatagtc ttaccagaaa ctctcttagt tgtagatatc atataccaac cctttgaaac
     2881 accgtttttg aaatgggcta gaagtcaggg caatccagca gtcaatggtc tgggaatgtt
     2941 gctctatcaa gctgcagaag cttttcaact gtggacaggc aaggaaatgc cgacagaaga
     3001 gatttggcag tctttaacag aaaaatacca ataaagaaag ggagactctc ctatgaaaat
     3061 cagaatcgat attcctcatc atccttatga tattcagatt gaaaaaggtt gtatggccca
     3121 ggctggtcag tggttgcgag aactctggca accgcaaaag gtagtcattg tgacagataa
     3181 ccatgtagcc tctctctatg cagagaaggt caagctcagc ctagaagatg ctggttttca
     3241 ggtagctgtt tttgatttct tagaaggtga agaaagaaag aatttaacta ctgttcagaa
     3301 agtctatgaa tttctagtca agcaaggtct gactcgtagc gatggaatcg ttgctcttgg
     3361 tggtggcgtt gttggggacc tggctggatt tgtagcctct acctatatgc ggggtattca
     3421 ctttgttcag attccgacta gtttgacagc tcaggttgat tcttctatcg gtggaaagac
     3481 aggcgttaac acaccatttg ctaaaaatat ggtggggacc tttgcccaac cagatggggt
     3541 tctgattgat ccacttgttc ttgaaaccct cggaaaaaga gagttgattg aagggatggg
     3601 tgaagttatc aagtatggct tgattgagga tccagaactg tgggctctct tgacggggct
     3661 gaatggttct gttgagagca ttttggaaca tgcagagacc ttgattgaac attcttgtca
     3721 ggtgaagcgc aagatggtgg ttgaagatga gttggacaat ggtattcgtc tttacctcaa
     3781 ttttggccac actattggcc atgccatcga agcgactgcc ggttatggca aggtcatgca
     3841 tggagaggct gttgccatgg gaatggtaca gatttccaag attgctgagg aaaaaggcct
     3901 catgccagct ggcattaccc aatctatcac agagatgtgt cagaaattcg gcttgcctgt
     3961 tgactatgaa aattgggaag ttgacaagct ttatcaggct cttactcatg acaagaaagc
     4021 gcgtggtaac accttgaaat tggtcttggt gccagagctt ggttcagcga ccattcatcc
     4081 agtttctctg gaagagatga aagactactt ggtaaaataa ggagagtgta tgagatattt
     4141 aactgcagga gaatcacacg gcccccgtct aacagctatt attgagggaa ttccagctgg
     4201 acttccattg acagctgagg atatcaatga ggaccttaga cgccgtcagg gtggctacgg
     4261 tcgtggtggt cgtatgaaga ttgagagtga ccaggttgtc tttacttcgg gcgttcgcca
     4321 cgggaagacg acaggggcgc ctattactat ggatgtcatc aataaggacc accagaaatg
     4381 gctggacatc atgtctgcgg aggacattga agaccgcctt aaaagcaagc ggaaaattac
     4441 tcatcctcgc ccaggtcatg ccgatttggt tggggggatt aagtaccgtt ttgatgattt
     4501 gcgaaattct ttggagcgtt catcagctcg tgaaaccacc atgcgggtgg cagttggtgc
     4561 agtagccaaa cgtctcttgg ctgagctgga tatggagatt gccaaccatg tcgtggtctt
     4621 tggtggcaag gaaatcgatg ttcctgaaaa tctgacagtc gctgaaatta agcaaagagc
     4681 tgcccagtct gaagtttcta ttgtcaacca agaacgagaa caggaaatca aggactatat
     4741 tgaccaaatc aaacgtgatg gtgataccat cggtggggtt gtggagacag tcgtcggagg
     4801 cgttccagtt ggtcttggtt cctatgtcca atgggataga aaattggatg caagattggc
     4861 tcaagctgtt gtctctatca atgcctttaa aggggtggaa tttggtcttg gctttgaggc
     4921 tggttatcgt aaaggcagcc aagttatgga tgaaattctc tggtctaaag aagacggtta
     4981 tactcgccgt accaataatc taggtggttt tgaaggtggt atgactaatg ggcaacccat
     5041 cgttgttcgt ggggtcatga aacccattcc tactctttat aaacctctta tgagtgtgga
     5101 tatcgaaacc cacgaacctt acaaggcaac cgtggagaga agtgatccga ctgctcttcc
     5161 agctgcagga atggtcatgg aagcagttgt agcaacggtt ctggcgcaag aaatcctcga
     5221 aaaattctca tcagataatc ttgaggaact aaaagaagcg gtagccaaac accgagacta
     5281 tacaaagaac tattaaggag ttcctatggc aaaaacaatc tatatcgcag gtcttgggtt
     5341 gattggagcc tctatggcac ttggtatcaa acgcgatcat ccagattatg aaattttagg
     5401 ttataatcgt agtcaagctt cgagagatat cgccttgaaa gaaggtatga ttgaccgtgc
     5461 aacggatgat tttgctagtt ttgctccttt ggcagatgtc attatcctca gcttgccaat
     5521 caaacaaact attgctttca ttaaggagtt ggccaatttg gatttgcgag aaggcgttat
     5581 tatttcagat gctggttcga ccaagtcaac cattgtggat gcggcggagc agtatttggc
     5641 tggcaagtct gttcgctttg tcggggccca tcccatggct ggtagtcaca agacaggggc
     5701 tgcttcggca gatgtcaatc tttttgaaaa tgcctattat atctttacac cttcaagcct
     5761 gacaagtcag gacacgctta aggaaatgaa ggatctgctt tcaggtcttc atgctcgttt
     5821 tatcgagatt gatgccaagg agcatgatcg tgtcacttct cagattagcc attttcctca
     5881 tattttggct tctagtctca tggagcagac tgcggtctat gctcaagagc atgagatggc
     5941 aaggcgcttt gcggcaggtg gttttcgaga tatgacccga attgcggaaa gcgagccagg
     6001 aatgtggacc tccattctct tgtccaatag cgagaccatt ctggatagaa ttgaggattt
     6061 caaggaacgt ttggaagcga ttggtcaggc cattagtaag ggagatgaag agcaaatttg
     6121 gaactttttt aaccaagcgc gtgaacaacg ccagaccatg gaaatccata agcgtggtgg
     6181 tgtggatagc tcttacgacc tctatgttga cgttcctgat gaagaagatg tcatcctgcg
     6241 gattttggaa ctgctacgtg gaacttcctt ggttaacatc cacatcaacg aggaaaatcg
     6301 tgaggatatt cacggaatcc tacaaatttc atttaaaaat gctcaagact tggaaagagc
     6361 tgagcatctc ataacagaaa ataccgacta tacagtcgtc atcaaataag gagaagaaca
     6421 tgtcaaatat ttacgatagt gcaaacgaac tcagtcgcgg tctacgcgga ttaccagaat
     6481 acaaggctgt taaagcagct aaagatgcga ttgcagcaga tgctgaggca agcaaaatct
     6541 ttacagaata tcttgccttt caagaggaaa ttcaaaaact agcacagaca ggtcaaatgc
     6601 cagatgcgtc cttccaagct aagatggaag gctttggtaa acagattcaa ggaaatagcc
     6661 tcttgtcaga attctttacc aagcaacaac aattggccat ttacctttct gacattgaaa
     6721 aaattgtttt tgaaccagtt tcagaattgc tgaaataaga aaagaacgat cctaaagtca
     6781 ggaattttta aggaatttct ggctttttat gataaaatag gtaaaagtat tgcaagtatg
     6841 aggtccagta tgaaactaaa aacaaacatt cgccatttac atggtagtat ccgcgtccca
     6901 ggtgacaagt ctatcagcca ccgttccatt atctttggaa gtttggctga gggtgagacc
     6961 aaggtttatg atattctgcg aggtgaagac gttctttcga ccatgcaggt ttttcgtgac
     7021 cttggtgttg aaattgagga taaagatggg gttattacca ttcaaggtgt aggcatggct
     7081 ggcttaaaag cgccacaaaa tgcccttaat atgggaaatt ctggcacctc gattcgcctg
     7141 atttcaggtg tccttgctgg tgcagatttc gaagtagaga tgtttggaga tgatagtctt
     7201 tccaaacgtc ctatggaccg tgtgaccctt ccactgaaaa aaatgggcgt cagcatttca
     7261 gggcaaactg aacgagactt gcctcccctt cgcttaaaag ggacgaaaaa cctaagacct
     7321 attcattatg agttgccaat tgcctctgcc caagtcaagt cagccttgat gtttgcagcc
     7381 ttacaagcta agggggagtc agttattatc gaaaaagagt gcacccgtaa tcatactgaa
     7441 gatatgttga aacaatttgg tggtcattta agtgtggacg gtaagaaaat cacagtccaa
     7501 gggccacaaa aattgacagg acagaaggtg gtcgtaccag gagatatttc cagtgcagcc
     7561 ttttggttag tcgcaggttt gattaatcca aattctcatc tagtgctgca gaatgtggga
     7621 atcaacgaaa cgcgtactgg tattattgat gtcattcgtg ccatgggtgg aaaattagaa
     7681 gttactgaaa tcgatccagt cgctaaatct tctaccttga ctgttgagtc ttctgacttg
     7741 aaaggaacag aaattggtgg cgctttgatt ccacgattga ttgatgaatt gcccattatt
     7801 gccctacttg cgacccaagc ccaaggtgta acagttatca aggatgctga ggagctcaag
     7861 gtcaaggaaa cagaccgtat tcaggttgtg gcagacgcct taaatagtat gggagcagat
     7921 attactccta cggcagatgg gatgattatc aaaggaaaat cagctcttca cggtgctaga
     7981 gtcaatacgt ttggtgacca ccgtatcggc atgatgacag ctatcgcagc cctcttggtt
     8041 gcagatggag aggtggagct tgaccgtgca gaagccatca ataccagcta tcctagtttc
     8101 tttgatgatt tggagagctt gattcatggc taaggtgtta ttagggttta tgggggctgg
     8161 aaaatcgact attgcaagag gcttggaccc taattacctt gatatggatg ctctgattga
     8221 gaatcgccta ggtatgtcca ttgcgaattt tttcgctgaa aagggagaaa cagcctttcg
     8281 tcaggtagaa tcagaagtcc tagctgattt actacaaaca gaccaagtcg tgtcaactgg
     8341 aggaggagtg gttatttctc agagaaatcg tgacttactc aagactaata cagataacat
     8401 ctacctgaga gcagattttg aaaccctcta ccaacgtatc gcagctgata aggacaatca
     8461 gcgaccgctt tttctaaata atagcaagga agaactagta gctattttcc aagaaagaca
     8521 ggcttggtat gaggaagtgg ctagtcgggt tttggatgtg accaagctaa gcccagagga
     8581 aattatagag gaactaagat gaaaattgct tatctaggtc ccaagggatc attttcacac
     8641 cacgttgtgc agacagcttt tcctcatgag gaattgcagg cttttgccaa cattacagat
     8701 gtcatcaagg cttatgagca aggattagtg aactattctg tggtgccagt tgaaaattct
     8761 attgagggta gtgttcatga aaccttggac tatctttttc atcaggctca catccaagca
     8821 gtagcagaaa tcgttcagcc tattcatcag cagttgatgg tggttccagg ccatactaag
     8881 attgaaaaga ttttttcaca tccacaggcc ttggctcaag gaaagaaatt catcgatgaa
     8941 caatatccag aggctcaaat cgaggtaaca gctagtacag cttatgcggc ccgttttatt
     9001 tccgaacatc cagaccagcc ctttgcagca gttgcaccta gaagttctgc tgaagaatat
     9061 ggattggaac tgattgccga ggatattcag gaaatggaag ccaatttcac acgtttctgg
     9121 gttctaggag ctgaaaagcc tagtattccc ttgcaagcac aaactgaaaa gatgagtttg
     9181 gccttgacat tacctgacaa ccttccaggt gcactttata aggccctgtc gacctttgct
     9241 tggcgaggaa ttgacttgac aaaaattgaa agttgtccac tcaagacagc actgggtgaa
     9301 tactttttca ttatcgatgt ggattatacc gataaggact tggtccactt tgcccaaaaa
     9361 gaattagaag cgattggaat ccagtataaa attctgggtg cctatcctat ttatccaata
     9421 tcagaccatg gaaaggagag aagatgagta aagaaaatcc cttaagtcat catgagcagt
     9481 tgcgttatga ttatttgcta aaaaatattc actatctcaa tgagagagaa aaaaatgagt
     9541 ttgcctattt gcaagaaaag ctaactcttg ctaggggaaa tagtagctct agcttggaac
     9601 aagaaagaga agagcaggtt gacttaccaa gctatgcgaa ccggagtcgc tcacaatcca
     9661 aatcacaagc actctctttc cctccaaaaa agaaaagacg gaagctccgt cttaaacgaa
     9721 tttttatggt gattttttca cttttggtct gtgtggcttt ggccatggta ttcatgtttt
     9781 tgcgtggtta ccaagatgct agcgcaaaga aaactgctga tgcccgggca gctcaagtag
     9841 aagtctttaa tggtcaggac actagagatg gagttaatat tttaatcatg ggtactgatg
     9901 gtcgaatcgg ccagaacagt gttgagacac gaactgactc tattatggta ttaaatgtcg
     9961 ggggctcaga taagaaaatg aagctagtca gtttcatgcg tgataatttg gtctatatag
    10021 atggttatag tcaagtgatt aatggtagaa aacagacaga taacaagtta aacgtagcct
    10081 acgagttagg agaacaagag gggcaaaaag gggcagaaat ggttcgccaa gtcttgaaag
    10141 ataattttga cttggacatt aagtactacg ccttggttga tttccaggcc tttgccacag
    10201 cgattgacac gcttttccct gatggggtga cgattgatgc tcaattttca acattgaatg
    10261 ggcgtcccct aacagaagct acagtcggag atgatttata cgctaccgag actgagtctc
    10321 caacccaaac cattaaagtc ggaaaacagc agatgaatgg ctcgaccctg ctcaattatg
    10381 ctcgtttccg tgatgatgat gaggcagatt acggccgtac aaaaagacag caacaagttt
    10441 taacagcaat tttagagcaa attaaagatc ccactaaact tttcactggt tctgaagctc
    10501 ttggaaaggt ttttgctatg acctcaacca acgtacccta tactttcctc ctaacaaacg
    10561 gtttatctgt tttggatgga gcaaaaaatg gtattgaaaa attgacgatt ccagaactgg
    10621 gtgactgggt agatgcctat gatgtttatg ggggcttggg cctgctggtt gatcaaaaca
    10681 aatatcaaac caagctcgct caaatgggct taagataaga taatactcaa tgaaaatcaa
    10741 agtgcaaact aggaagctag ccgcaggctg ctaaaaacac agttttgagg ttgtagataa
    10801 gactgacgaa gtcagttacc tatacctacg gcaaggtgaa gctgacgtgg tttgaagaga
    10861 ttttcgaaga gtataagaca gaaaaatcaa agtttgatag ctatttggat agcagtcagg
    10921 ctttgatttt ttacagtctg caatagattt tcaccccttg tttgtggtat aatgaaacga
    10981 tacgatagaa agaataatga acaatatgac tgatttaaaa gcaattcagg ctcgtagtct
    11041 ggagatggct gaatattttg tggccttttg taaagaacat gatttgctct gttatctctg
    11101 tggcggaggc gctattggtg cccttcgaaa caagggattt attccttggg acgacgacct
    11161 agactttttt atgcctcgta aagattatga gaaattagca gaattatggc ctcgttatgc
    11221 agatgaacgt tatttcttgt caaagagtca caaggatttt gttgatcgta atctttttat
    11281 taccattcgt gacaagaaaa ccacctgtat caagccttat cagcaggatt tggatttgcc
    11341 acatggtctg gccttggatg ttttgccttt ggattattat ccgaaaaatc cagctgagcg
    11401 gaaaaaacag gttcgttggg ccttgattta ttcactcttt tgtgcgcaaa ctattccaga
    11461 aaagcatggt gatctcatga aatggggaag tcgcatttta ctgggtttga ctccaaaatc
    11521 tctccgttat cgcatctgga aaaaagctga gaaagaaatg actaagtatg atttggctga
    11581 ttgtgatggc attacagaat tatgctcagg tcctggctac atgagaaaca agtacccaat
    11641 cacatctttt gaagacaatc ttttcttgcc atttgaagga acagagatgc ctattccaat
    11701 cggctatgat gtctatctca gaactgcttt tggggattat atgacgcctc caccagcaga
    11761 caagcaggta ccgcatcatg atgctgtcat cgctgatatg gataagtctt atacagaata
    11821 caagggagaa tatggtggct aagaaaaaaa tcttattttt tatgtggtct ttttctcttg
    11881 gaggtggtgc agagaagatt ctatcaacca ttgtttcaaa tctggatcca gaaaagtatg
    11941 atattgatat tcttgaaatg gagcactttg acaagggata tgaatctgtt ccaaagcatg
    12001 tacgcatttt aaaatccctt caagattatc gccaaaccag atggttacga gcttttttgt
    12061 ggagaatgag aatttatttt ccaagactga ctcgtcgttt gcttgtaaaa gatgattatg
    12121 atgttgaagt ttcttttacc attatgaatc caccactgtt gttctctaaa agaagagaag
    12181 tcaagaagat atcttggatt catggaagta ttgaagaact tcttaaggat agctctaaaa
    12241 gagaatcaca tagaagccag ttggatgctg cgaatacaat tgtagggatt tcaaaaaaga
    12301 ccagcaattc tatcaaggaa gtttatccag attatacttc taaattacag acaatctaca
    12361 atggatatga ttttcagact attctagaaa aatctcaaga gaagatcgat atcgagattg
    12421 ctcctcaaag tatctgtact atcggacgga ttgaggaaaa taagggttct gaccgtgtag
    12481 tggaagtgat acgattatta caccaagagg gaaaaaacta tcatctctat tttatcgggg
    12541 ctggtgatat ggaagaggaa ctgaaaaaac gagtcaaaga gtatgggatt gaggactatg
    12601 tacatttcct tggttatcaa aaaaatcctt atcagtatct atctcagacg aaagttcttt
    12661 tgtctatgtc taaacaagaa ggttttcctg gagtgtatgt ggaggccttg agtctgggac
    12721 tcccttttat ctctacggac gttggagggg ctgaggaatt atcccaagaa ggacgatttg
    12781 gacaaatcat tgagagcaat caagaggcag ctcaggcgat tactaattac atgacttctg
    12841 cctcaaactt tgatgtcgat gaggctagcc aattcattca acaatttaca attacaaaac
    12901 aaatcgaaca agtagaaaaa ctattagagg agtagcatgg aaactgcatt aattagtgtg
    12961 attgtgccag tctataatgt ggcgcagtac ctagaaaaat cgatagcttc cattcagaag
    13021 cagacctatc aaaatctgga aattattctt gttgatgatg gtgcaacaga tgaaagtggt
    13081 cgcttgtgtg attcaatcgc tgaacaagat gacagggtgt cagtgcttca taaaaagaac
    13141 gaaggattgt cgcaagcacg aaatgatggg atgaagcagg ctcacgggga ttatctgatt
    13201 tttattgact cagatgatta tatccatcca gaaatgattc agagcttata tgagcaatta
    13261 gttcaagaag atgcggatgt ttcgagctgt ggtgtcatga atgtctatgc taatgatgaa
    13321 agcccacagt cagccaatca ggatgactat tttgtctgtg attctcaaac atttctaaag
    13381 gaatacctca taggtgaaaa aatacctggg acgatttgca ataagctaat caagagacag
    13441 attgcaactg ccctatcctt tcctaagggg ttgatttacg aagatgccta ttaccatttt
    13501 gatttaatca agttggccaa gaagtatgtg gttaatacta aaccctatta ttactatttc
    13561 catagagggg atagtattac gaccaaaccc tatgcagaga aggatttagc ctatattgat
    13621 atctaccaaa agttttataa tgaagttgtg aaaaactatc ctgacttgaa agaggtcgct
    13681 tttttcagat tggcctatgc ccacttcttt attctggata agatgttgct agatgatcag
    13741 tataaacagt ttgaagccta ttctcagatt catcgttttt taaaaggcca tgcctttgct
    13801 atttctagga atccaatttt ccgtaagggg agaagaatta gtgctttggc cctattcata
    13861 aatatttcct tatatcgatt cttattactg aaaaatattg aaaaatctaa aaaattacat
    13921 tagaacgggg gtgaccaagt gattgatggg aaacgattat tatttagttt gaccatagtc
    13981 agttatgcct tgacgctagt aagtggaatt gtgtatctgt ttaataataa taatgttagc
    14041 ttactttcta ctttattgtt cttgttggtt agtagcttaa ttgcttgttg gaatgatatc
    14101 aagtattact taatccattt tattttctat ttaaccattt ttgtatttct ggtatcaaga
    14161 ccgaccattg attattttag ggatggtgct ttggatacct atcatccaat agcctatcgt
    14221 tttgccttta tagttgtcat gatttcgatt ctgggcttga ccacaggagg cattctggct
    14281 cgttacttca tagctaggaa gaaaataaaa gtagcaaata taggaaattc tctaaaagag
    14341 gtttatatca agcggttacg ctttgtatca ctaggagttt ttcttctaac ttatcctttc
    14401 tatttcattc ggttatttga acggctcttg tatcgtttgc agacttccta ctatgcctac
    14461 tatgcaaatt ttgaaagtaa actgccttat tttacctaca ttttgtctac ctttacggtc
    14521 tatgcaatgt gtatgtatct ggcaaccaag ccaaagaaat tgcaggccac agcagtgctt
    14581 gtctccttta ttgcagctaa tactattcat ttggcaattg ggacacgaaa tccctttatt
    14641 ttaagtattt tatttgcttt tgtttattac tttatgcggg agcaaactga aaaaggaaaa
    14701 tggattgggt ttaaagaaaa gttagcgatt tttgtaggtt ctcctattct catgttagcg
    14761 atgggagtac tcaattatgt acgggataat gtccaagttt cccatacagg tttctgggat
    14821 atcttacttg actttatcta taaacaaggg actagttttg gtgttctggc tcgaggtttt
    14881 ctatttaaca gtagcctccc ttaccgagat ttccgtaatt ttacttttgg tcctgttctt
    14941 gattattttg caagggggag tttgggagcc attttcggag gaaaagcctt tgaacataca
    15001 accaatagtg tggaactagc tattgatagt aatagttatg cccacaatct atcctatctt
    15061 gtcttgaaca aggaatactt gaaagggcat ggtatcggaa gtagttatat catggagttg
    15121 tataccgact atggtatgat tggagtcttt ctgcttagtt tcttactcgg cgtattattt
    15181 atagccatgc tgcaagtagc ctatcgctca aggacaatcc tatttgcttt atccctactc
    15241 atcttgaata atctattctt tatgccaaga agcagctttt cagaaagttt cttcaattta
    15301 tttacaatgc aattctgggg aattgttctt gtgattatat ttgtagcaaa aatgcttaca
    15361 aaggaaaact agtatctact aaacaaagga gaaaaaaatc atgtttgaac attattcagt
    15421 agctgatttg tttgcaaatc tttataaaaa gcgaaaagca aatattttag ctctcatcgc
    15481 tttatttgct ctcattgctg taccatttac aattaaagca gttaggaata aaaacactgt
    15541 caaagataca acaagttatt caacttatct tatctataaa atcactcctc caaaagagtc
    15601 ggccaaaacg attttgaatc atcaaattgg tggttatagt gatttttatg ggaaattgat
    15661 tgatggtaat ttgaatggag cttatctttt caatgatgta gaacccagtg agttgaaaaa
    15721 aattgccagt gaattagaca cgacagaaac aaccttgaaa aattcaacga atgactattg
    15781 gtggaaaaaa ttgaccgtct actatatgat tgacgatgca ggggttggtg tgaaaatttt
    15841 gacatcaagt aaagatgcca ataacttgtt agagaaaaaa attgatgggt tgattgagaa
    15901 atttaaacat gcctatgcaa atgtgaaaat tgaaaaactg gaaaccatca actctaaaga
    15961 attgaacgca aatggtgaaa cagcgcttgg cttaaatgtg aaaaatctga ttcttcgttt
    16021 agttgttatt ggagtggttt gtgtgatttt ggttgtgatg ggaaatgtgt tagtttatct
    16081 ctttaatcca acaatcaata gagtaggtga tttttctcag tatcaaattg attttgtaac
    16141 agagattaca acaattgcta acctagcaga tgttttgtca tacaaaaata ctggacagga
    16201 attgaccatc gttagctcaa ataaagctat cttagataaa ttgaaacaga gtcaagaagc
    16261 tttaaaagga atgcattttg tagatttaca ggatgtatca tctcttttgg aaagagatac
    16321 agtccttctt gttgaagagt acggagtgac tcgttataag aaatttgagc aaagtcttca
    16381 aattctcaga aacttaaatc gttctatcct cggtgtagca acctttaaat tataagtatt
    16441 ctgatgtata aggtcttcaa agtcggctct tagccgattt tgaaggcttt ttctatctgt
    16501 ctgcagaaat tttcttttgg agtctcctat agaaagaccc tagggaaaat ctatccgtat
    16561 agaggagcct tcttttgata aaatcgtaaa aatctagtat aatagataga actgaaagta
    16621 tgaggttact agcaatgaaa atgaaacaaa ttagtgatac aactttaaaa atcacgatat
    16681 ctttagagga tttgatggat cgtggaatgg agattgctga ctttctcgtt cctcaagaaa
    16741 aaacagaaga gttcttttat gctatcttgg atgagctaga gatgcctgat agctttctgg
    16801 atacaggcat gttgagcttc cgtgtgactc caaaacctga taaggtagat gtctttgtga
    16861 ccaagtcaaa gattgaccaa aatctagatt ttgaagactt atcggatttg ccagatatgg
    16921 aagaattggc tcaaatgtct ccagatgaat ttatcaaaac cctggaaaaa agcatcgcag
    16981 acaaaaccaa ggatgatatc gaagccattc aatctcttga gcaagttgaa gtcaaggaag
    17041 aagagcaaga gcaggctgaa caagaagctg agagtaagaa agaaccttac atctactaca
    17101 tcctttcttt tgctaagttg gctgacttgg tagcttttgc caagacagtg acttttgaaa
    17161 tggaaacttc tgaactctac aaaatgaacg agcgctatta tttgaccatt ttagtggata
    17221 ttgaaaatca tccaagccca tatccagctt ggctgttggc ccgtatgcgc gagtttgcag
    17281 acgatagtga tatcagtcgc tcagtcttac aagagtatgg tcaagtcttg atgagtcacg
    17341 atgcagtgct caatctgcaa aaaatcggct aatcttttct ggaaagctat tttcagattt
    17401 taagaagagc gaatcgtctg attcgttttt tctttttata ctgaaatagt gatttactat
    17461 aataggaatt ttcacaaaat tctgttataa tggctatatt agaaaatttc gaggagataa
    17521 agatgacagt taaaattgct ttactaggat ttggtaccgt tgcaagtggt gtgcctttcc
    17581 tcctaaagga aaatggagga aaaatcaatc aatcagcaca ttcagatatc aaagttgcta
    17641 aggtattggt caaggatgaa gatgaaaaaa atcgcttgct tgcagcaggg aatgacttta
    17701 actttgtaac caatgtggat gatattttat cagaccagga tattactatc gtagtggaat
    17761 tgatggggcg tattgagcct gctaaaacct ttatcactcg tgccttggaa gctggaaaac
    17821 acgttgttac tgctaacaag gaccttttag ctgtccatgg cgcagaattg ctagaaatcg
    17881 ctcaagctaa caaggtagca ctttactacg aagcagcagt tgctggtggg attccaattc
    17941 ttcgtacttt agcaaattcc ttggcttctg ataaaattac gcgcgtgctt ggagtagtca
    18001 acggaacttc caacttcatg gtgaccaaga tggtggaaga aggctggtct tacgatgatg
    18061 ctcttgcgga agcacaacgt ctaggatttg cagaaagcga tccgacgaat gacgtagatg
    18121 ggattgatgc agcctacaag atggttattt tgagccaatt tgcctttggc atgaagattg
    18181 cctttgatga tgtagcccac aagggaatcc gcaatatcac accagaagac gtagctgtag
    18241 ctcaagagct tggttacgta gtgaaattgg ttggttctat tgaggaaact tcttcaggta
    18301 ttgctgcaga agtgactcca accttcctac ctaaagcgca cccacttgct agtgtgaatg
    18361 gcgtaatgaa cgctgtcttt gtagaatcta tcggtattgg tgagtctatg tactacggac
    18421 caggtgcggg tcaaaaacca actgcaacaa gtgttgtagc tgatattgtc cgtatcgttc
    18481 gtcgtttgaa tgatggtact attggcaaag acttcaacga atatagccgt gacttggtct
    18541 tggcaaatcc tgaagatgtc aaagcaaact actatttctc aatcttggct ctagactcaa
    18601 aaggtcaggt cttgaagttg gctgaaatct tcaatgctca agatatttcc tttaagcaaa
    18661 tccttcaaga tggcaaagag ggtgacaagg cgcgtgtcgt tatcatcaca cacaagatta
    18721 ataaagccca gcttgaaaat gtctcagctg aattgaagaa ggtttcagaa ttcgacctct
    18781 tgaatacctt caaggtgcta ggagaataag atgaagatta ttgtacctgc aaccagtgcc
    18841 aatatcgggc caggttttga ctcggtcggt gtagctgtaa ccaagtatct tcaaattgag
    18901 gtctgcgaag aacgagatga gtggctgatt gaacaccaga ttggcaaatg gattccacat
    18961 gatgagcgta atctcttgct caaaatcgct ttgcaaattg taccagactt gcaaccaaga
    19021 cgcttgaaaa tgaccagtga tgtccctttg gcgcgcggtt tgggttcttc cagctcggtt
    19081 atcgttgctg ggattgaact agccaaccaa ctgggtcaac tcaacttatc agaccatgaa
    19141 aaattgcagt tagcgaccaa gattgaaggg catcctgaca atgtggctcc agccatttat
    19201 ggtaatctcg ttattgcaag ttctgttgaa gggcaagtct ctgctatcgt agcagacttt
    19261 ccagagtgtg attttctagc ttacattcca aactatgaat tacgtactcg cgacagccgt
    19321 agtgtcttgc ctaaaaaatt gtcttataag gaagctgttg ctgcaagttc tatcgccaat
    19381 gtagcggttg ctgccttgtt ggcaggagac atggtgaccg ctgggcaagc aatcgaggga
    19441 gacctcttcc atgagcgcta tcgtcaggac ttggtaagag aatttgcgat gattaagcaa
    19501 gtgaccaaag aaaatggggc ctatgcaacc tacctttctg gtgctgggcc gacagttatg
    19561 gttctggctt ctcatgacaa gatgccaaca attaaggcag aattggaaaa gcaacctttc
    19621 aaaggaaaac tgcatgactt gagagttgat acccaaggtg tccgtgtaga agcaaaataa
    19681 agaatagaag ataggatggg gaaactcttg accagagggt ttcatatcct ttttgtgaaa
    19741 agaagtttat actcaatgaa aatcaaagag caaactagga agctagccgc aggctgctca
    19801 aaacagtgtt ttgaggttgt ggatagaact gaagaagtca gctcaagaca ctgttttgag
    19861 gttgcagata gaactgacga agtcagtaac atatatacgg taaggtgacg ctgacgtggt
    19921 ttgaagagat tttcgaagag tattagttaa aaacgtgata aaggagaaat aaagatggca
    19981 gaaatttatc tagcaggtgg ttgtttttgg ggcctagagg aatatttttc acgcatttct
    20041 ggagtgctag aaaccagtgt tggctacgct aatggtcaag tcgaaacgac caattaccag
    20101 ttgatcaagg aaacagacca tgcagaaacg gtccaagtga tttacgatga gaaggaagtg
    20161 tcactcagag agattttact ttattatttc cgagttatcg atcctctatc tatcaatcaa
    20221 caagggaatg accgtggtcg ccaatatcga actgggattt attatcagga tgaagcagat
    20281 ttgccagcta tctacacagt ggtacaggag caggaacgca tgctgggtcg aaagattgca
    20341 gtagaagtgg agcaattacg ccactacatt ctggctgaag actaccacca agattatctc
    20401 aggaagaatc cttcaggtta ctgtcatatc gatgtgaccg atgctgataa gccattgatt
    20461 gatgcagcaa actatgaaaa gcctagtcaa gaggtgttga aggccagtct atctgaagag
    20521 tcttatcgtg tcacacaaga agctgctaca gaggctccgt ttaccaatgc ctatgaccaa
    20581 acctttgaag aggggattta tgtagatatt acgacaggtg agccactctt ttttgccaag
    20641 gataagtttg cttcaggttg tggttggcca agttttagcc gtccgatttc caaagagttg
    20701 attcattatt acaaggatct gagccatgga atggagcgaa ttgaagttcg ttctcgttca
    20761 ggcagtgctc acttgggtca tgttttcaca gatggaccgc gggagttagg cggcctccgt
    20821 tactgtatca attctgcttc tttacgcttt gtggccaagg atgagatgga aaaagcagga
    20881 tatggctatc tattgcctta cttaaacaaa taaaacagag agtggggctt cccactttct
    20941 tcatttctag aatatgaata gaagggattt atgaaacacc tattatctta cttcaaaccc
    21001 tacatcaagg aatcaatttt agcccccttg ttcaagctgt tagaagctgt ttttgagctc
    21061 ttggttccca tggtgattgc tgggattgtt gaccaatctt tacctcaggg agatcaaggt
    21121 catctctgga tgcagattgg cctgctcctt atctttgcag taattggcgt tttagtggcc
    21181 ttgatagctc aattttactc agcaaaggca gcagtaggat ttgctaagga attgacaaac
    21241 gatctttatc gtcatattct ttccttgcca aaggacagca gagaccgtct gacaacttct
    21301 agtttggtta ctcgcttgac ttcggatacc taccagattc agactggtat caatcaattc
    21361 ctgcgtctct ttttacgagc gcccattatc gtttttggtg ccatttttat ggcttatcga
    21421 atctcagctg agttgacttt ctggttctta gtcatggttg ccattttgac cattgtcatt
    21481 gtagggttat ctcgattggt caatcctctc tacagtagtc tcagaaagaa aacggaccaa
    21541 ctggttcagg aaacgcgcca gcaattgcaa gggatgcggg ttattcgtgc ttttggtcaa
    21601 gaaaaacgag agttacagat ttttcaaacc cttaaccaag tttatgctag attacaagaa
    21661 aagacaggtt tctggtctag tttattaaca cctctgacct atctgattgt caatggaact
    21721 cttctcgtta ttatctggca aggctatatt tcaattcaag gaggagtgct cagtcaaggt
    21781 gctctcattg ctcttatcaa ttacctctta cagattttgg tggaattggt caagctagcc
    21841 atgttgataa attctctcaa ccagtcctat atctcagcca agcgaatcga ggaggtcttt
    21901 gcggaatctc ccgaaaacat ccattcagaa ttagaacaaa agcaagttac cagtggtcgg
    21961 gttttacaag tccaagaatt gacttttacc tatcctgatg cggcccagcc ttctctgaga
    22021 tacatttcct ttgatatgac tcaaggacaa attctaggta tcatcggggg aacgggttct
    22081 ggtaagtcaa gcttggtgca actcttactt ggactttatc caatagataa ggggagcatt
    22141 gacctttatc gagatggatc tagtcctcgt aatttagagc agtggcggtc ttggattgct
    22201 tatgttcccc aaaaggtcga actttttaag ggaactattc gttccaactt gactttaggt
    22261 ttaaatcaag aagtgactga tcaagaactc tggcaagcct tggaaattgc tcaagctaag
    22321 gattttgtca gtgataagga aggactcttg gatgctctag ttgaagcagg agggcgaaat
    22381 ttctcaggtg gacaaaaaca aaggctgtct atcgcacgag cagtcttgcg ccaggctccg
    22441 tttctcatcc tagatgatgc gacctcagca ctggacacca ttaccgagtc caagctcttg
    22501 aaagctatta gagaaaattt tccaaacatg agcttaattt tgatctctca acgaacctcg
    22561 actttacaga tggctgacca gattctcctc ttggaaaaag gtgagttgct agctgttggc
    22621 aagcacgatg acttgatgaa atccagtcaa gtctatcgtg aaatcaatgc atcccaacat
    22681 ggaaaggagg actagaatga aacgacaaac tgtaaaccag acgctcaaac gtttagccgt
    22741 agatttagca aaccatccct tcctcctttt cctagccttt ctaggaacta ttgcccaagt
    22801 tggcttatca atttatctac ctattctgat tgggcaggtc attgaccaag tcctagtggc
    22861 tggttcatca ccagtttttt ggcagatttt tctccagatg ctcttggtgg taataggaaa
    22921 tactctagta caatgggcca atcctctcct ctataatcgt ctaatcttct cttataccag
    22981 agatttacga gagcgaatca tccataaact ccatcggtta ccgattgctt ttgcggatcg
    23041 gcaaggcaat ggagagatgg tcagtcgtgt aaccacggac atcgaacagt tggcagctgg
    23101 cttgaccatg atttttaacc aatttttcat tggtgttttg atgattttgg ttagtattct
    23161 agccatgctc caaattcatc tcctcatgac tctcttagtc ttgctgttga cgccactgtc
    23221 catggtgatt tcacgcttta ttgccaagag atcctatcat ctcttccaga agcaaacaga
    23281 gacgagggga attcagactc agttgattga agaatcgctt agccagcaga ctataatcca
    23341 gtccttcaat gctcaaacag aatttatcca aagattgcgt gaggctcatg acaactactc
    23401 aggctattct cagtcagcca tcttttattc ttcaacggtc aatccttcga ctcgctttgt
    23461 aaatgcactc atttatgccc ttttagctgg agtaggagct tatcgtatca tgatgggttc
    23521 aaccttgacc gtcggtcgtt tagtgacttt tttgaactat gttcagcaat acaccaagcc
    23581 ctttaatgat atttcttcag tgctagctga gttgcaaagt gctctggctt gcgtagagcg
    23641 tatctatgga gtcttagata gccctgaagt ggctgaaaca ggtaaggaag tcttgaccag
    23701 tgaccaagtt aagggagcta tttcctttaa acatgtctct tttggctacc atcctgaaaa
    23761 aattttgatt aaggacttgt ctatcgatat tccagctggt agtaagatag ccatcgttgg
    23821 tccgacaggt gctggaaaat caactcttat caatctcctt atgcgttttt atcccattag
    23881 ctcgggagat atcttgctgg atgggcaatc catttatgat tatacacgag catcattgag
    23941 acagcagttt ggcatggtgc ttcaagaaac ctggctcaca caagggacca ttcatgataa
    24001 tattgccttt ggcaatcctg aagccagtcg agagcaagta attgctgctg ccaaagcagc
    24061 taatgcagac tttttcatcc aacagttgcc acagggatac gataccaagt tggaaaatgc
    24121 tggagaatct ctctctgtcg gccaagctca gctcttgacc atagcccgag tctttctggc
    24181 tattccaaag attcttatct tagacgaggc aacttcttcc attgatacac ggacagaagt
    24241 gctggtacag gatgcctttg caaaactcat gaagggccgc acaagtttca tcattgctca
    24301 ccgtttgtca accattcagg atgcggattt aattcttgtc ttagtagatg gtgatattgt
    24361 tgaatatggt aaccatcaag aactcatgga tagaaagggt aagtattacc aaatgcaaaa
    24421 agctgcggct tttagttctg aataagccat tctcttttga aagtttatgg acgaaaaaag
    24481 ttgccttcga gtgacttttt tgttacaata gctagaaaaa ttgtttactg taatactcaa
    24541 tgaaaatcaa agagcaaact aggaagctag ccgtaggttg ctcaaaacac tgttttgagg
    24601 ttgcagatag aactgacgaa gtcagctcaa aaaactgttt tgaggttgca gatagaactg
    24661 acgaagtcag taaccacacc tacggcaaag tgaatttgaa gtggtttgaa gagagtacaa
    24721 cttgtctttt agaaaaggag cctataatga aagtctttca gcatgtaaat atcgtgactt
    24781 gtgatcaaga tttccatgtt tatcttgatg gaatcttagc agtcaaggat tctcaaatcg
    24841 tctatgtcgg tcaagataag ccagcgtttt tagagcaagc tgagcagatt atagactatc
    24901 agggagcttg gattatgcct ggtttggtca attgtcacac ccattctgca atgacaggcc
    24961 tgagagggat ccgagatgac agcaatctcc atgaatggct caatgactat atctggccag
    25021 cagaatctga gtttactccc gacatgacta ccaatgcggt caaagaagcc ctaacagaga
    25081 tgctccagtc aggaacaaca acctttaacg atatgtataa tcccaatggt gtggatatcc
    25141 agcaaattta tcaggtggtg aaaacttcca agatgcgttg ttatttttct ccgactctct
    25201 tttcttcaga gacagaaaca actgctgaga ctataagcag aactcgatcc atcatagacg
    25261 aaatcttaaa atataaaaat ccaaatttca aggttatggt agcacctcat tctccgtata
    25321 gctgcagtag agacttgctg gaagcgagtt tggaaatggc aaaagagcta aatattccgc
    25381 tccatgtcca tgtggcggag accaaggagg agtcaggaat tatcctcaaa cgatacggca
    25441 aacgccccct tgcttttctt gaagaactgg gttatttaga tcatccgtcc gtctttgctc
    25501 acggggtcga attaaacgag agagaaattg aacgcttggc atcttctcaa gtggctatcg
    25561 cccacaatcc tatcagtaac ctcaaactgg catcaggaat tgctccaatt atccagctcc
    25621 aaaaagcggg agtagcagtc ggaattgcga ctgactcggt tgcttccaat aacaatctag
    25681 atatgtttga ggaaggaagg actgcagctc ttcttcagaa gatgaaaagt ggggatgcca
    25741 gccagtttcc aatcgaaaca gctctcaagg tactgacaat cgaaggggct aaggtccttg
    25801 gaatggaaaa tcagatagga agtctggaag tcggcaagca agcagatttt ctggtcattc
    25861 aaccacaagg gaaaattcat ctccaacctc aggaaaatat gctgtctcac ctggtttatg
    25921 cagttaaatc tagtgatgta gatgatgttt atatcgccgg agaacaggtt gttaagcaag
    25981 gtcaagtcct gacagtagaa ctttaaaaga aaaattacga aaaattttaa aaaaagtttg
    26041 caaaaatctt gcattctttt tttgactatg ctatacttat atacggttta aaaaaactgc
    26101 ctaagacagt aggggagctc gactcataaa tatcctaccg aggacaaaac gtatcatgta
    26161 aaaagaagcg tattgtactt tcgtgtctag gtttgggcgc gtttttcttt ttgaaaaatt
    26221 ccccaagcaa aataattacg gaggtgaaca cactaatgag tgaagcaatt attgctaaaa
    26281 aagcggaact agttgacgta gtagctgaaa aaatgaaagc tgctgcatct atcgtcgttg
    26341 tagacgctcg tggtttgaca gttgagcaag atacagttct tcgtcgtgag cttcgtggaa
    26401 gcgaagttga gtataaagtt attaaaaact caatcttgcg tcgtgcagct gaaaaagctg
    26461 gtcttgaaga tcttgcatct gtatttgttg gaccatctgc agtagcattt tctaatgaag
    26521 atgttatcgc accagcgaaa atcttgaacg acttttctaa aaacgctgaa gcacttgaaa
    26581 ttaaaggtgg tgcaatcgaa ggcgctgtcg catctaaaga agagattctt gcacttgcaa
    26641 ctcttccaaa ccgcgaagga cttctttcta tgctcctttc tgtacttcaa gcgccagtgc
    26701 gcaacgttgc tcttgcagtc aaagcggttg cagaaagcaa agaagacgcg gcttaatctt
    26761 aagctacata gcgtagccta gctacgaaaa aaactattat aaaatttaaa acttatttgg
    26821 aggaaataac aatggcattg aacattgaaa acattattgc tgaaattaaa gaagcttcaa
    26881 tccttgaatt gaacgacctt gtaaaagcta tcgaagaaga atttggtgta actgcagctg
    26941 ctcctgtagc tgttgctgca gctgatgctg ctgatgctgg tgctgctaaa gattcattcg
    27001 acgttgaatt gacatctgca ggcgacaaaa aagttggtgt tatcaaagtt gtacgtgaaa
    27061 tcactggtct tggtcttaaa gaagctaaag aacttgttga cggtgcacca gcacttgtta
    27121 aagaaggcgt tgcaactgca gaagctgaag aaatcaaagc taaattggaa gaagctggag
    27181 cttcagttac tcttaaataa tagagtatcg caatttgatt gcggaatccc tatttaccag
    27241 tctttgagag cgataagcga ttgatagaaa ctgataaatt gatttggttt cctgccaaaa
    27301 attctttggc aggatttttg ttttaataaa ctttcggggg tagacactag attgtctacc
    27361 cctttttttg tgtttcggag gtaactatga aaagaataat tccagtttat atattccaac
    27421 aagtaaatgt cctattggta tctctatact tattgaaatt tctttgtatc ggtgagttaa
    27481 ctatactaca ggttctctat ggtgcgtctc ttttttcttt tttatggatg tatggtcaac
    27541 gaaaacaagt ggtcaaggtc aatatgaaaa ctaggatgaa atgccttggt attggactag
    27601 ctagtctact gattataagt ctatgtttta gcctgattca tgctcaagga agcacgaatc
    27661 aaacaaattt aattggcctt caacatcaag ttccttggtt ttcattttta ttgttcttaa
    27721 tcaatgcgag tatggttgaa gaatttctgt atcgtgaaat tttatggaac ttggttagaa
    27781 aattagatat tcgagttgct ttgacaagtg ttttatttgc cttagtacat catccaggaa
    27841 ccattctagc ttggtgtctg tacgtttcac ttggaatgtt tttaggtatg gtgcgctata
    27901 aatctgactt atggggaagt atggggttac atttggtgtg gaacttttta gtttatagtt
    27961 tgctgctttt ttaaaatagg tgacctgatt tatctcataa atctgtagtt tttatgg
//
LOCUS       DAVHEX010000023        27756 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVHEX010000023 DAVHEX010000000
VERSION     DAVHEX010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27756
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.023"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(88..324)
                     /locus_tag="VVL45_000995"
     CDS             complement(88..324)
                     /locus_tag="VVL45_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU4187186.1"
                     /translation="MDVILSAIIFGISHLILSHRDPISLLYYSLIGFFFALVYRSTDN
                     LRLTILCHSFFNFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(337..810)
                     /locus_tag="VVL45_000996"
     CDS             complement(337..810)
                     /locus_tag="VVL45_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187187.1"
                     /translation="MRNTKKLRQFGIFLLIILLSTYLPQTIGLYVTIILGLGADVYSL
                     ILTMGLVGSFLLLIWRLKKKKMLFIFEKKSWNWSFVFYLFATYVVYQILGNFWARYAH
                     LINHRNIHDEYFTVLFSNGQPTFLSTILSFVLPVIIGPVFEETLDRGYFMNTFFP"
     gene            complement(828..1013)
                     /gene="briC"
                     /locus_tag="VVL45_000997"
     CDS             complement(828..1013)
                     /gene="briC"
                     /locus_tag="VVL45_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HEU4187188.1"
                     /translation="MTGTNTFTVLSTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRAG"
     gene            complement(1172..1939)
                     /locus_tag="VVL45_000998"
     CDS             complement(1172..1939)
                     /locus_tag="VVL45_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEU4187189.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEIGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            complement(1936..2802)
                     /locus_tag="VVL45_000999"
     CDS             complement(1936..2802)
                     /locus_tag="VVL45_000999"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEU4187190.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            complement(2839..4206)
                     /gene="accC"
                     /locus_tag="VVL45_001000"
     CDS             complement(2839..4206)
                     /gene="accC"
                     /locus_tag="VVL45_001000"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEU4187191.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            complement(4218..4640)
                     /gene="fabZ"
                     /locus_tag="VVL45_001001"
     CDS             complement(4218..4640)
                     /gene="fabZ"
                     /locus_tag="VVL45_001001"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEU4187192.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVVIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGTLTFAIGN"
     gene            complement(4637..5122)
                     /locus_tag="VVL45_001002"
     CDS             complement(4637..5122)
                     /locus_tag="VVL45_001002"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEU4187193.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVATEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            complement(5125..6360)
                     /gene="fabF"
                     /locus_tag="VVL45_001003"
     CDS             complement(5125..6360)
                     /gene="fabF"
                     /locus_tag="VVL45_001003"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU4187194.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            complement(6382..7113)
                     /gene="fabG"
                     /locus_tag="VVL45_001004"
     CDS             complement(6382..7113)
                     /gene="fabG"
                     /locus_tag="VVL45_001004"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEU4187195.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVVAIDGGLSM"
     gene            complement(7147..8067)
                     /gene="fabD"
                     /locus_tag="VVL45_001005"
     CDS             complement(7147..8067)
                     /gene="fabD"
                     /locus_tag="VVL45_001005"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEU4187196.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            complement(8060..9034)
                     /gene="fabK"
                     /locus_tag="VVL45_001006"
     CDS             complement(8060..9034)
                     /gene="fabK"
                     /locus_tag="VVL45_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEU4187197.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     ISIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVD
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWTGVVRND"
     gene            complement(9153..9377)
                     /locus_tag="VVL45_001007"
     CDS             complement(9153..9377)
                     /locus_tag="VVL45_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU4187198.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            complement(9437..10411)
                     /locus_tag="VVL45_001008"
     CDS             complement(9437..10411)
                     /locus_tag="VVL45_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEU4187199.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGEELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            complement(10411..10845)
                     /locus_tag="VVL45_001009"
     CDS             complement(10411..10845)
                     /locus_tag="VVL45_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU4187200.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            complement(10939..11724)
                     /locus_tag="VVL45_001010"
     CDS             complement(10939..11724)
                     /locus_tag="VVL45_001010"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEU4187201.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            12049..13413
                     /locus_tag="VVL45_001011"
     CDS             12049..13413
                     /locus_tag="VVL45_001011"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEU4187202.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKNQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            13416..13787
                     /locus_tag="VVL45_001012"
     CDS             13416..13787
                     /locus_tag="VVL45_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEU4187203.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            14000..15274
                     /gene="serS"
                     /locus_tag="VVL45_001013"
     CDS             14000..15274
                     /gene="serS"
                     /locus_tag="VVL45_001013"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEU4187204.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGADEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSAAKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(15299..16198)
                     /locus_tag="VVL45_001014"
     CDS             complement(15299..16198)
                     /locus_tag="VVL45_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEU4187205.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLIGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(16308..16856)
                     /locus_tag="VVL45_001015"
     CDS             complement(16308..16856)
                     /locus_tag="VVL45_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU4187206.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            complement(17074..18396)
                     /locus_tag="VVL45_001016"
     CDS             complement(17074..18396)
                     /locus_tag="VVL45_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEU4187207.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVVLLGIG
                     TFTQVNSIAESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMALIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGVRFIWLYRVVFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     regulatory      complement(18487..18575)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(18739..21075)
                     /locus_tag="VVL45_001017"
     CDS             complement(18739..21075)
                     /locus_tag="VVL45_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEU4187208.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QSFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVYFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(21179..21727)
                     /locus_tag="VVL45_001018"
     CDS             complement(21179..21727)
                     /locus_tag="VVL45_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEU4187209.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(21724..22026)
                     /gene="zapA"
                     /locus_tag="VVL45_001019"
     CDS             complement(21724..22026)
                     /gene="zapA"
                     /locus_tag="VVL45_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEU4187210.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSHEIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            22113..22994
                     /locus_tag="VVL45_001020"
     CDS             22113..22994
                     /locus_tag="VVL45_001020"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEU4187211.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TPDQHDFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANRFSNPISLEEKAEG
                     KYLAVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLSFC
                     AKLHFKNTEKAKKRLER"
     gene            22998..23612
                     /gene="lepB"
                     /locus_tag="VVL45_001021"
     CDS             22998..23612
                     /gene="lepB"
                     /locus_tag="VVL45_001021"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU4187212.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            23846..26212
                     /locus_tag="VVL45_001022"
     CDS             23846..26212
                     /locus_tag="VVL45_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEU4187213.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLAKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVRILEKGKQKSHDLKTIQKHITTIEKELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDLRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSEESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKGDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDSIPAMIGITDTDLKEIFGK"
     gene            complement(26260..27543)
                     /locus_tag="VVL45_001023"
     CDS             complement(26260..27543)
                     /locus_tag="VVL45_001023"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEU4187214.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDQKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVITAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
BASE COUNT         8092 a         6360 c         5380 g         7924 t
ORIGIN      
        1 ggatttaccc actacaaata ttatagagcc aacaataaaa agaaaaagtg tttgatagat
       61 atcaaacact tttttctttg cctcccacta tctaaaaaaa tgataataga tataattgta
      121 aacaaaaatc cagataggtt ttgcatgatt gagaaagtta aaaaaactat ggcagagaat
      181 cgttaatctc agattgtcgg tagaacgata aacaagggca aaaaagaaac caatcagact
      241 ataatataat aaactaattg gatctctgtg agatagtatc aaatggctaa tcccaaagat
      301 gatagcagat aggataacat ccaaatagta cttggactag ggaaagaagg tattcataaa
      361 ataccctcta tcaagagtct cctcaaaaac aggaccgatg attacaggca ggacaaaaga
      421 taagatagtc gataaaaagg ttggttgtcc atttgaaaaa agcacggtaa aatactcatc
      481 atgaatattc ctatgattaa tcaaatgagc atagcgtgcc caaaaattac cgagaatctg
      541 ataaaccaca taagttgcaa ataagtagaa gacaaatgac cagttccagc tctttttctc
      601 aaagataaag agcatctttt tcttttttaa cctccaaatt aatagaagga aacttcccac
      661 taatcccatt gttaaaataa gagaatagac atcagctcct aaccctaaaa tgatcgtcac
      721 atacaatcca attgtttgtg gtaaataggt agatagtaaa ataataagca aaaatattcc
      781 aaattgtctt agtttttttg tgtttctcat cgtacttttt tgaaagatta ccctgctcgg
      841 aagccgtact tccaagcatc tatataagaa ttaagtgccc cttgcctcat atagggagca
      901 aattctctat aatataacca tctactatat ccatcttccc aaacagcaag accacctgaa
      961 gtttgctcca agtcctcagt tgaaagaact gtaaatgtat ttgtacctgt cattgcaagt
     1021 accttcttaa aatagattgt tgtaggctca catttatagt atatttcttt ttttgtctat
     1081 tttatagccc atctcctcaa ctggcaattt ttcgacctga attacatttt tccataaaaa
     1141 atgagacctt tctagtctca tttagtcatt cttagtattt tctaaatcgt tgatagcgtt
     1201 cttccagcaa ctcttctagc ggtttttgtg aaagtctagc cagctccgtt tggagttctt
     1261 ttttgacact cttaatcagt tctttactag aaagtcctat ttcagaaatc accttatcca
     1321 ccacgtccat ttctaacagt tcatgcgaag tgattttcat cagttctgct gcttccatag
     1381 cgcgagtacc gtccttccat aaaatggaag caaagccttc tggactgaga atggcataga
     1441 tagaattttc cagcatccag acacggtccg cgacagctag agccagagcc ccgcctgaac
     1501 caccttcacc gataataatg gcgataatag gaactttcag gtcactcatt tccatgagat
     1561 tgcgagcgat agcttcccct tgaccacgtt cttccgctcc gacaccagga taagcacctg
     1621 ctgtattgat aaaggtcaca actggacggc caaatttctc agcctgtttc atcaaccgca
     1681 gtgcctttcg gtagccttct ggatgtggtt ggccaaaatt ccgtttgagg ttgtcttgca
     1741 aactcttgcc tttttggata ccaaccactg ttacagcttg gtctccaagc caaccaatac
     1801 caccaacaac tgcaccatca tcacgaaaag aacggtcacc atgtaattgg ataaattcat
     1861 caaaaatgcc tgtcgcaaag tccaaggttg tcaagcgact ctgctcacgc gcttctctga
     1921 ctatttttgc aatattcatc taggactccc tccatgcaat ctgactaggc tagcaatcgt
     1981 atctggtaag tctcttcttt tgacaatagc atccacaaag ccatgttcta ataggaattc
     2041 tgccttttgg aaatcctcag gcaagctttc acgaaccgta ttttcaatca cacgacgccc
     2101 agcaaaacca accaagctct gtggttcagc cagaatgata tcgccttcca tagcgaaaga
     2161 agctgtcaca ccaccagtcg ttggatctgt caaaatggtc aggtaaaaga gaccagcatt
     2221 tgaatggcgt ttaaccgccg cagagatctt agccatctgc atgagactca tgattccttc
     2281 ctgcatacgg gctccaccag aggctgtgaa taggacaact ggcaattttt cgacagtcgc
     2341 atactcaaac aaacgagtga ttttttcacc tacaaccgta cccatagaag ccatgataaa
     2401 gttagaatcc ataatcccaa gagccacagt ctgaccttta ataagagcag ttcctgtcac
     2461 aacggcttca tgcagacctg ttttttcacg catagatgcc agtttctttt ggtaaccagg
     2521 gaaatgcaag ggatccttgc tttcaatccc tgtaaacaat tctttgaagg ttcccatatc
     2581 aatcgtcaaa gccaagcgtt cttgggcaga aatacgaaag gtatagctac agtgcggaca
     2641 gatacgttca cttcccagat ccttctgata gatggtatgc ttacagcctg gacactggga
     2701 aaataattca tctggaacct ctggcttagc ttgaggtttt tccctaaccg aacgattggg
     2761 attgattcga atatacttat cttttttact aaatagagcc attgattccc cttttcggtt
     2821 taaactctta aagtcatttt attctttttc ttgatattta ggtaagaagg tttccatcaa
     2881 gaaggaagta tcataatccc cagcaatgac attgcgatct gaaatgaggt caagctggaa
     2941 atctgcattg gtctgcactc cttcaatttc taattcatag agggcacgtt gcattttcat
     3001 caaggcgtca aaacgatttt cgccgtgtac tatgattttg gcaatcatac tatcataata
     3061 aggcggaatg gtataacctg gataaactgc tgaatccacg cgcaagccaa ctccaccact
     3121 tggcagatag agattagtaa tcttacctgg acttggagca aagttaaagg ctgggttttc
     3181 tgcattgata cgacactcga tggcatgacc gcgtaggaca atatcttctt gcttaacaga
     3241 caaaggctga cctgccgcaa tgcaaatctg ttccttaacg atatcaacac ctgaaacaaa
     3301 ctctgttact ggatgttcta cctgaacacg agtattcatc tccatgaaat agaaattgct
     3361 acttgcttca tcaagaagaa attcaatggt tcctgcattc tcatagccaa caaactctgc
     3421 cgctcgaaca gcagcagcac ctatttcatg acgcagcgtt tttccgattg caatcgaggg
     3481 actttcttcc aaaacctttt ggttattcct ttgaagagaa caatcccgtt cacccaagtg
     3541 aatcacatgt ccatgctcat cacctaggat ttgaacctca atgtgccgag ctggatagat
     3601 aacccgttct atgtacatgg caccattgcc ataattggcc ttggcctcac tagaggcagt
     3661 ttcaaaggca gaaacgaggt catctggttt ttcaacctta cgaatccctt taccacctcc
     3721 acctgctgaa gccttgagca taacaggata gccaattttt tcagcaacaa tcaaagcttc
     3781 ttcagagtta tgcacttctc catctgaacc tggtataaca ggcacacctg ctttaatcat
     3841 ctgagcacgc gcattgatct tatcccccat catatccata acatgaccag atggaccgat
     3901 aaacttgata cctacttctt cacacatggt cgcaaatttg gaattttcac tgagaaatcc
     3961 aaaaccaggg tgaatagctt ctgcctcagt caagactgca gctgatagaa ctgcattaat
     4021 attgagataa gactctgttg ccttgccagg accaatacaa actgcttcat ctgccaaaag
     4081 cgtatgaaga gcttccttat cagcagttga ataaaccgct accgtcgcaa tccccaattc
     4141 acgtgccgca cggataatac gaaccgcaat ttcaccacga ttggcaatta aaatttttcg
     4201 aaacatggag aacctcctta gttcccaatt gcaaaagtaa gggtaccact ggctgcaagc
     4261 ttgccatcca cttcagcctt tgcttcaacc acagctatgg tgccacgacg ttttacaaaa
     4321 gtcgctgtca taaccaattg gtcgcctggt acaacttgct tcttgaactt aaccttgtcc
     4381 ataccagcgt aaaagaccag ttttccttta ttttcaggtt ttgataactc caacacaccg
     4441 gcagtttgcg ccaaggcttc cataatcaca acacctggca taactgggta ttgaggaaag
     4501 tggccgttaa agaaaggctc gttgatggtc acatttttga tagcaacaat ggtatcctcg
     4561 ctcacttcca agacacggtc cactagaagc ataggataac ggtggggaag agcttctttg
     4621 attccttgaa tatcgatcat ttgatacgta ccaatccttt accaaactca accatttctt
     4681 cgttagagac gagaatttcc gttaccacac catccttagg agctgggatt tcattcatga
     4741 ctttcatggc ttcgataatt accaatgttt gacctttttt gacactatca ccaactgtaa
     4801 cgaaggcagg tttatctggt ccagcagcca agtaaaccac tccaacaagt ggactctcta
     4861 caagatttcc ctcagtagcc acacttgctt cagctggagc tggaacttct tctgctacag
     4921 tctctgctgg agcagatgta ggagctactg gactcggtgt tgctagaacg ggtgctggag
     4981 cgacttgagt tgcaacttca ggcacaggtc ttgcttcatt cttgctaaac tgcaactcat
     5041 ccgtcccatt tttataagaa aattctctca aacttgactg gtcaaattga gtcatcaagt
     5101 ctttaatatc gtttaaattc atacttatct attctcccaa cgtttgaaag caagaactgc
     5161 attgtggcct ccaaaaccaa aagtatttga aatagcgtat ggaatttctt tctccaagcc
     5221 ttgtccataa acgacattag cttcgatata atctgatact tcacttgtcc cagctgtcat
     5281 tggtacaaag ttatgacgca tagcttcgat ggtgacgata gcttctactg cacccgcagc
     5341 ccccagcaaa tgtcctgtaa aagacttggt tgatgataca ggtacttcct taccaagaac
     5401 agctacgata gcaccacttt ctcctttttc attggcagga gttgacgttc cgtgagcatt
     5461 gacataggct acttgctctg gagaaatctc agcttcttcc aaggctagtt tgatggcctt
     5521 gatagctccc tgaccttctg gatgtggaga agtcatgtgg taggcatcac aagtatttcc
     5581 gtaaccaacc acttcagcca ggatagtagc tccacgtttt tcagcgtgtt caagactttc
     5641 tagaaccaac atccctgaac cttcacccat aacaaaccca ttgcgatcct tatcaaatgg
     5701 gatcgaagca cgagttggat cctctgtagt agagagagct gttaaggctt ggaaaccagc
     5761 gatggcaaaa ggtgtgatag aagcttctgt tcctcccacc aacatcacat cttggaaacc
     5821 aaacttaatg gagcggaagg catccccaat cgcatcattt gatgaagagc aggcagtatt
     5881 gatagattta caaacaccgt ttgcaccaaa acgcatggct acattcccag aagccatatt
     5941 tggtaaagct tttggaagag tcattggttt gacacgtttg ggtccttttt catgaaggcg
     6001 aagtacctga tcttcaattt ccttgattcc accaatacca gatgcaacga taacaccaaa
     6061 acgatcccta ttaagagcct ctacatcaag attggcatga tttacagcct cttgggctgc
     6121 atacaaggca tataaagaat agttatcaaa acggttggta tcttttttta caaagtattt
     6181 atcgaacgga aaatcttgga tttctgccgc attatgcaca tcaaagtcac tatgatcaaa
     6241 ttttgtaatg ccaccaatgc cgattttccc agttgctaaa ctattccaaa attcttctgg
     6301 tgtatttccg attggagatg ttactccata acctgttacc actactcgat ttagtttcat
     6361 tcttttcacc tctagctttc gctacatact taagccacca tcaatggcaa ccacttgtcc
     6421 agttagataa tcttggcctg ctaaaaatac tgtcaaatct gcaacctgct ctgcctgccc
     6481 aaattctttc atcggaatct gagctagtgt agcttcctta atcttatctg acaggatagc
     6541 ggtcatatca gactcaatca ttcctggagc aatcacattg actcgtatat tccgactagc
     6601 gacctcgcgt gccacagact tggtaaagcc aatcaagcca gccttagaag cagcataatt
     6661 agcttgacca atattcccca tcaaaccaac aacactagac atattaatga tagcaccttc
     6721 tctggctttc atcatcggtt tcaagactga ttgtgtcata ttaaaggcac cagtcagatt
     6781 gaccttgagc actttttcaa aatctgcttc tgtcatcttg agcataagag tatcttgggt
     6841 aatccctgca ttgttgacca aaacatctac tgaacccagt tctgcaatag cttgatcaat
     6901 catacgctta gcgtctgcaa aatctgatac atctcctgaa atgggaacca ccttgatacc
     6961 atagtttgaa aactcagcga gcaattcttc tgagattgcc ccacgactgt ttaagacaat
     7021 gttggctcct gcttgagcaa acttgtgggc gatggcaaga ccaattccac gactcgaacc
     7081 tgtaataaag atatttttat gttctagttt catttttttc ctttcaaaac ttctacttat
     7141 tttagtctat ttttctaaaa gtgctactaa actcgcttga tcttccacat gagctaagtg
     7201 agcagtttga tcaatttttt taacaaaacc tgacaagact tttcccggtc caatctcgat
     7261 aaagttgctt atgcctgctt cttgcatgac cccaatactt tcatagaaac gaacgggttc
     7321 cttgacctga cgcgtcaaga gctgagcaat gtcctctttt tgcatcacag cagcttctgt
     7381 attgccgact aggggacaag taaaatctga aaaacttacc tgagctagag tttcagctag
     7441 tttctggcta gcaggttcaa ggagagcggt gtgaaaggga cctgacacct taagaggaat
     7501 caagcgtttg gcacctgctt cttgcaaaag ttcaaccgct cgatcaactg caaccacttc
     7561 tccagcaatg acgatttgtg caggtgtgtt atagttggct ggagtaacca ctccaagttc
     7621 agaagctttt tgacaggctt cttcaatgac ctctactggc gtattgagaa ctgctaccat
     7681 cttgccagag tcagcaggag ccgcttcttc catataggct ccacgcttag ctaccaaggc
     7741 aaccgcatct tcaaaatcca aggcgccact tgccaccaag gcagagtatt ctccaagaga
     7801 caaaccagca accatatcag gctgatagcc cttttcttgc aataaacggt agatagcaac
     7861 cgaagtcgct agaatggctg gttgcgtata gcgggtctga ttgagtttgt cttcttccgt
     7921 atcgatgaga taacgcaaat cataaccgag cacctggctc gctcgatcaa tcgtttcttt
     7981 aacaatcgga tactgatcat agaaatcccg tcccatccct agatactggg caccttgacc
     8041 agcaaataaa aaggctgttt tagtcatttc ttacaactcc tgtccagcga gaggcttctt
     8101 cttgaatttt cttagcggct ccgtaataca aatcttttag gatttcttca gctgtttctt
     8161 ctttagaaac aagccctgcg atttgacctg ccataacaga gccaccatcc acatcaccgt
     8221 gaacaactgc tttggctaga gcacctgctc ccatttgttc aaagatttct aaatcaggat
     8281 cttcttgctt aaaggcatct ttttcagcca gttcaaaatc tctagtcaac tgatttttaa
     8341 tagcacgaac agcatgacca aagtgctgag ctgaaatcgt agtatcaata tcccttgctt
     8401 ttaaaatttt ctccttgtag tttggatggg cattcgactc ttttgcaact acaaaccgtg
     8461 tccccacctg tacagcctct gcacctagca taaagccagc cgcagcacct tcaccatccg
     8521 caattcctcc tgcagcaata acaggaatag atatagctgt ggctacctgt cgcaccaagg
     8581 tcatggttgt taatttaccg atatgccccc cagcttccat tccttctgca ataacagcgt
     8641 ctgcaccgat tttttccatg cgtttagcta aagcgacact aggaacaaca ggaataacga
     8701 ttatcccagc ttcatggaaa cgttccatat acttgcttgg atttcctgct cctgttgtga
     8761 caactttaac accttcttca ataacgagat ccacgatgtc ttccacaaag ggagataaga
     8821 gcatgatgtt gaccccaaag ggtttatcag tcaatgattt gattttatca atattggcct
     8881 tgacaacttc tttcggggca tttcccccac cgataattcc taatcctcca gccttggaaa
     8941 cagcccctgc caaatcacca tcagcaaccc aggccatccc tccttggaaa ataggataat
     9001 caatcttcaa taattctgta atacgcgttt tcatagtgcc tccaaccttc cttgcttacg
     9061 taatagttcg atttcaccat aatttgacag tcaaactatt acctaaacaa gagggagtgg
     9121 gtttctccct actccttcta ctaatattct gcttattttg cttgctcttc aacgtaagca
     9181 accaagtcac caactgtttt caagtcattt tctgcttcga tttggatatc aaaagcatct
     9241 tcgatttctg agattacttg gaacaagtcc aatgaatctg cgtccaaatc atcaaaagtt
     9301 gattcaagtg ttacttctga tgcgtctttt ccaagttctt caacgataat ttcttgtact
     9361 ttttcaaata ctgccatgat aggactcctt taaaataaat agttttttta taacaatgtg
     9421 ttcaccacat gattacctaa attgtaagaa tgagcgtgcc ccaggtcaag cctccaccga
     9481 agcctgatag aagaacagtc tggctaccat ctaaagggat gagaccttgt tctacacact
     9541 ctgaaagtaa aatcgggata ctggctgcac tggtattgcc atattccatc atattggctg
     9601 gaagtttggc tcggtcaaca ccaatttttc tagccatctt atccaaaata cggtcattgg
     9661 cttgatgaag tagcagataa tccaagtctg tcacctctat aggagattca tcaatagtct
     9721 gcttgataga cttggctaca tctcgaatgg caaaatcaaa gactgtgcgt ccatccatct
     9781 tcaaaaacga atctgcactt tcttgatctg aaaatggaga atgtaaacct gaatgcccat
     9841 aagttaaaca ctcgctgcga cttccatcgc tattgagact ctcagctaag aaatgctctt
     9901 gctcgctagc ttctaacaag acaccaccag caccatctcc aaacaacaca gctgttgatc
     9961 gatccgacca atcgactgcc ttagagaggg tttcactacc aatcaccaag cctttttgaa
    10021 agcgaccaga agcgataaac ttttcagcag ttgaaagagc aaatacaaat ccactgcaag
    10081 ccgcggttaa gtcaaaagca aaggctttat tagcaccaat attagcttga acacgagcag
    10141 ctgtagaggg catcatcgaa tctggagtaa tggtagctag gatgataaaa tccagttctt
    10201 ctcctgttat tccagctttt gccatcagtt tcttagcaac ctctgtagcc aaatcactgg
    10261 tagattctgt tcttgaaata tgcctttgtc gtattcccgt tcgacttgaa atccactcat
    10321 cattggtatc cataatctga gccaagtcgt gatttgtaac cacttgctct ggcacataat
    10381 gagcaacctg acttattttt gcaaaagcca ttatttcaaa tcctccaaaa attggtaaag
    10441 attagtcaaa cctttaccca tgacagcaat ttcttcctcg ctcatgccat caataatttt
    10501 ttctaccatg gccttgtgga agcgtttatg cagtctatga atcaagcgac ccttctttgt
    10561 caaatgcaga tgcaccacac gacgatcctg ttctgaccga actcgctcaa tgtagccctt
    10621 acgctctaaa ttgttcaaac ttgtcgtaac agttccaaga gttaccatca actcttttga
    10681 cacttgactt ggagtcacgt ctggagcctt accgatgaca tcaatcgtat gcatttcttt
    10741 gatggagata tccttaaaac gactacctct caagttcact tcctcaatta caaggacatt
    10801 gttaaatata gatgttaaat attcattgat tcgttggtag tccatttttc atatccctcc
    10861 ttcttcaaaa aacttttaca atcaaaacat ttgacagaaa gaattatatc aaaattcaaa
    10921 gaatgatgca agtatttttt attttcctgt aaatttaggt cttcttctct ccgaatgagc
    10981 tcgcactcct tctttgaaat cctctgtttg agctaaggat ttctgtaggt tcagttctaa
    11041 agtagcgtaa ccctgccagt ctttaaattg gctctcccaa accaacttct taatggcagc
    11101 ataggaatta cttgaagcac gtcttaattt tttaagaagc tgttctctcg tcttttcaag
    11161 tttttcagct tcagaaacgc ggtaaaccaa cccccattct agggcttttt ctgccgttaa
    11221 agcctctcct gtcatagcta attgagcagc acgcgtcaca ccgatactgc ggctcaagag
    11281 atgaatcccc cctgcatctg gagccaaacc aacaccaaca aaggcttgga taaacttagc
    11341 cttatccgtc gccaaacaga aatctgccgc aacagccata ttcgctgcgg cacctgcaac
    11401 agcaccgtca acttccatta aaacaggttt ggctatttgc ttgattttat aagaaatcgt
    11461 attgaccaac tctgcgattt ttgtcaatga tggaatatca tcctcatcca ctgcccgctt
    11521 catctctacc aaatctcccc caactgagaa gacctttcca ttggcattga ttaagataaa
    11581 atgcacagct ggattttctt ctgccaaagt cagagcttct aaaatctcct cacacatggg
    11641 aatatgaaaa ccatttgcga cctcaggacg gttcaaggta aggattgcca agtcctcttc
    11701 aagttgataa ataatgtgtt ccatcagggc tcctttttat tttataagca gtttatactc
    11761 ttcgaaaatc tctttaaacc gcgtcagctt ccatctgcaa cctcaaaaca gtattttgag
    11821 ctgacttcgt ctagcttctt agtttgctct ttgattttca ttgagtgtta aattatttca
    11881 ctgtcaaagt atatcttttt taggagcaga aagcaagaaa aaatcgactg aaagtgtctc
    11941 agccgataag aaaggctcgc tttaggctag aaagcatctt attttcttca ttgaaaaaag
    12001 cctttctttc tgctataatc gtataaaata cttactttag gagttcttat gaaagttgtt
    12061 aaatttggag gtagttctct tgcctctgct agtcaattag aaaaagtttt aaacatcgtc
    12121 aaaagcgatt cagagcgtcg ttttgtagtc gtttctgcgc ctggtaaacg caatgctgaa
    12181 gatactaagg ttacggatgc cctgattaaa tactaccgcg actatgttgc gggtaacgat
    12241 attagcaaga accaaagctg gattatcgac cgctatgctg ctatggttag tgaattggga
    12301 ctaaaaccag ctgtgctaga aaaaatttct aaaagcattc acgccttggc cactcttcct
    12361 attgaagaaa atgaatttct ctacgatact ttcctagcag ccggtgaaaa taacaatgcc
    12421 aaattgattg ctgcctactt taaccaaaat ggtatcgatg cacgctatat gcaccctaga
    12481 gaagctggga ttgtggtcac aagtgaacct ggtcacgctc gcatcattcc atcaagttat
    12541 gacaagattg aagaattgac aaacaccaat gaagtccttg tcattcctgg tttctttggt
    12601 gtcactaagg aaaatcaaat ctgtactttc tcacgtggag gttctgatat tacaggttct
    12661 atcattgctg ctggtgtcaa agctgacctc tatgaaaact ttacggacgt tgatggtatc
    12721 tttgcagccc accctggtat tatccaccaa ccacactcga ttcctgagtt gacctaccgt
    12781 gaaatgcgcg agttggccta tgcaggcttc tcagtccttc atgacgaggc tcttcttcct
    12841 gcctaccgtg gaaaaattcc tctggttatc aagaatacca acaaccctga ccatccaggt
    12901 actcgtatcg ttctaaaaca cagtaatgat gaatttccag ttgtgggaat tgctggtgac
    12961 tcaggctttg tcagcattaa catgtcgaaa tacctcatga accgtgaggt tggatttggc
    13021 cgcaaggttc tgcaaatcct ggaagaactt aacatcggtt gggaacatat gccaacaggt
    13081 atcgacgatc tttctatcat tctccgttct cgccaactaa ctcccatcaa ggaagaagaa
    13141 atcctgcgtc agttggttca aaaggctaaa gtagatcatg cagaaatcga acacgacctt
    13201 tctatcatta tgattgttgg tgaaaaaatg aagagccata tcggagtgac tgctactgcg
    13261 acacgcgctc tatctgaaaa caaaatcaac atccagatga tgtctcaagg ttctagtgaa
    13321 gtttctatta tgtttgttgt caataaagac caagagaaag cagcaattaa agccctctac
    13381 aatgcctttt ttggagaaag taaggaagac taagcatggc ccaatcactt aacaaaacag
    13441 tactcctcag tacgacaggc acttcctacc tctctatcgc tgggaaagtt gggaaattcc
    13501 ttgtcggaga tcaggctttg gaattttacc cagatgtcaa tgtcgaacaa tttatccaga
    13561 ttccttggag ccatatcaat cagattggag ccaatgtcac tggacgcaaa atcagccgcc
    13621 acttcgaagt ctttacagac aaaggaaaat tcctctttgc ttcaaaagac tcaggtgcca
    13681 ttctcaaaat tgcacgcgaa aaactaggca atgacaaggt cgtcaaactt ccgaccctga
    13741 ttcagacaat tagccaaaaa tttaaaaatc tatttgcaaa aaagtagaaa ctttagtata
    13801 atcatagcaa cggataagta ggttatcttc ttctttcaga aagtctgcgg gtactgcgag
    13861 cagataggag ggataggtga aattctaccg ttagtgacaa ttacatacac aatcaagtgc
    13921 acacctgtga agttggatgg aaccgtggcc ctgccactcc aacgttttgt cagggtgctt
    13981 ttttcataaa ggagttctta tgttagatat caaacgtatt cgtacagatt ttgaagctgt
    14041 cgcagaaaaa ttagctacac gtggtgtaga tgctgctgtc ttgaatgaaa tgaaagaaat
    14101 cgatgctaaa cgtcgtaaca tcttggtcaa ggttgaaact ctcaaagcag aacgtaacac
    14161 agtttctgct gagattgccc aagctaagcg caacaaggaa aatacagatg acaagattgc
    14221 tgccatgcaa aatctatctg ctgaggttaa agccttggat gctgaattgg cagaaatcga
    14281 tgctaaattg acagaattta caacgactct tccaaatatc ccagctgaca gcgttcctgt
    14341 tggggctgac gaagacgaca atgtggaagt tcgccgttgg ggtactccac gcgagtttga
    14401 cttcgaacct aaagctcact gggatctcgg tgaagacctt ggtatccttg actgggaacg
    14461 cggtggtaag gtaacaggcg ctcgcttcct cttctataaa ggcctcggtg ctcgtttgga
    14521 acgtgctatc tacaacttta tgttggatga acatggaaaa gaaggctata ctgaagtcat
    14581 cacaccttac atagtcaacc atgattctat gtttggtact ggtcagtatc caaaatttaa
    14641 ggaagatact tttgaactca gcgataccaa ctttgtcttg attccaactg ctgaagttcc
    14701 tctgacaaac tactaccgtg atgaaatctt agacggcaaa gatcttccaa tctacttcac
    14761 tgccatgagt ccgtcattcc gttctgaggc tggttctgcc ggtcgtgata cgcgtggctt
    14821 gatccgtttg caccaattcc acaaggttga aatggtcaaa tttgccaaac cagaagaatc
    14881 ttacgaagaa ttggaaaaaa tgacagccaa cgctgaaaac attcttcaaa aactcaacct
    14941 tccataccgt gtcgttgctc tctctactgg agatatgggc ttctcagctg cgaagactta
    15001 cgacttggaa gtgtggattc cagcacaaaa caattaccgt gaaatctcaa gctgttcaaa
    15061 cacagaagat ttccaagccc gtcgtgccca aatccgttac cgtgatgaag cagatggcaa
    15121 ggtgaaactc cttcatacct tgaacggttc tggacttgca gttggacgta cagtggctgc
    15181 aattcttgaa aattaccaaa atgaagatgg ttctgtgacc atcccagaag cacttcgtcc
    15241 atacatgggt ggagctgaag tcatcaaacc ataaaaaata aggtttagct atttctagct
    15301 agaccttttt tcgtaaccaa atcagataag cacctagtac aaagaataaa atagttaggc
    15361 atataatggt ttcagccaat accaggtaat ccagaaatgg aagtttcaaa attccctgag
    15421 ccatcttgag cgaggtcgct gtgataatgg ttgggaaggt gagggctgag aaggctggtt
    15481 gaaaaccttg ttttaaaatg ttgggcagac gagttaaaac aaagaaaaag aaggattgag
    15541 aagccaaaat catgacaatc aagacccaag tcggcaggct ggttcctcct actcgaacta
    15601 gagaagccaa gagtagagag aaaggagcac agtagattcc ttcttgtcca agcaaggcta
    15661 gtgggagtgg atgtttcttt aaatcgctat aaataagggg atagagatag aaggtcaaga
    15721 gaaaaccaaa actcaaggtc gcataggcaa tttcgataat acctaccaga ggataggtca
    15781 aggcagccac tgctatcccc acatagagaa ccgtccagct tggagtggca tgaaccctcc
    15841 gccctggaca agcaaacttg atggtaaaac cagcaatcaa ggtcaaatcc aagagaaatg
    15901 aaaaccacca aatcccttgt gctaccaaag gaagataaga gaatacgcga aagacatagg
    15961 tcgataaaat catcccagcc ataggaaagg ttgccattcc tgacaaaaga gggggcttgg
    16021 tcaattcttg cttggtttct ttccaattaa agagatgcag aattagaaag taaatccata
    16081 aaaccaaacc aatcagacta aaaagatggg atagaaccgg caacgtatct aaaataagat
    16141 ttccagctcc tgccaaacct agcaaacaac ctgaaaatac taaggggagt tttttcatcc
    16201 taacctccaa taatcatgtt agtttcagta taacataaaa gcgcttaaat gaggatttaa
    16261 aaaaacgaag tccgcttatt tcagacttca ttttactcag atatgaatta ggcataaggt
    16321 tgcaattctg gattaattgg tgtattagct aagttgttgg catagttaca gaggattgct
    16381 aggctgacac caaaaaccac atccaaggca ttttgttgag tgtagccagc ttctaaaaac
    16441 tcagacaagg cttcatctcc tacacgaccc ttggtattga taactgccaa ggtaaactta
    16501 gctagggtat ccaatttagg atctgtttca attggagtac gattgcgaag agcttgaatc
    16561 aagtcatcat tcatctggat ttgtttgatg gaaaaggctg tgtgacctgc gacacagaag
    16621 gcacaaccat tggtcacggc tgccgtgatt tgcaccactt cacgctcaac gggtgtcagg
    16681 ctgttgcgac ggtggataga tgagacaatt tggtaggctt ctaaaacagt cggggcattg
    16741 gccaagagac cgattaggtt gggaatatag ccattgttgt ctttttctac tgtttcaaga
    16801 atttctttca cttctgctgg tgctgactct actgtatgga tagtaaatgt tgtcataaga
    16861 tacctctttt cttattattg acactaatat tattggaaaa tcttataaaa tcctgattcc
    16921 taagtttatc taagataaag ctttattctc tcataagatt ttcgttgtta tattagttta
    16981 tcacacttcc aatcacttgt ataatatata ttatatatca ggctgataaa aattatttat
    17041 aggcaaaaaa atcacacgag ctgtgtgatt ccattatttg tcaaaatact ttttagtttc
    17101 agcaataacg actggcgaca agaccaagag ggcaatcaag tttggcagag ccatcaaggc
    17161 gttaacgata tctgcgataa tccagaccat atccaactcg ataaatcctc ctaacaagac
    17221 catgagcaca aaaaccacac ggtagagcca gataaagcga accccaaaga ggaactcaaa
    17281 acagcgttct ccgtaatagt tccaacctag aatcgttgta aaggcaaaaa gtacaaggaa
    17341 gatggtcaag agggcaggcc caaagtgtga aaagactgtt gagaaagctg actgagtcaa
    17401 ggcaacccca ttcaagtcac cactccaaac tccagttacc aagatggtca aaccagttag
    17461 agtacaaatg atgagggtat caataaaggt tcctgtcatg gaaatcaaac cttgctctac
    17521 tggttcattt gtcttggcag ctgcagctgc aataggagca gaacccagac ccgattcgtt
    17581 tgagaacaca ccacgcgcca caccattttg aatagccatc cgaacgctag caccagcaaa
    17641 tccacctacc gcagcaaggg gactaaaagc tgaggtaaag actaaagcga ttgtgccagg
    17701 gatttttccg atattaaaga aaataactgt aagagttcct aagatataaa tgagggccat
    17761 aaaaggaaca acagtagttg aaaccttaga aatagacttg agtccaccaa agactgcaat
    17821 cgctacaaag acagacaaga cgagagctgt gatggctggc gaaatcgtcg ttgtattttg
    17881 gatagattct gcaatcgagt tgacttgggt gaaggttccg attcccaaga gaacaaccaa
    17941 tactcctgct actgcaaaca aaacagcaag tggtcgccac ttttctccca tccctagaag
    18001 gatatagtgc atgggacctc ccgctactgc accatggtcg tccttggtgc ggtatttgat
    18061 ggccaagagt ccttccgcat acttggtagc cattccaaag aaagccgcca tccacatcca
    18121 aaatagagct cctggtccac caaccttgat agccgtcgca actcctatga tatttcctgt
    18181 tccaacagtt gatgccaagg ctgtacacag agctgcaaaa ctggatacat caccatgtcc
    18241 cttatcctgg ataaaaataa gctgaaaggc cttgggcaga cgcaaaacct gcaagagtcc
    18301 tagccgcatg gttaggtaaa tccctgttcc gaccaataaa atcaagaggg gcggtcccca
    18361 agcaaaagca tcgattgatt taagcaattc taacatttcc ttctcctatc gtttcaaccc
    18421 caaaagaaag agcacatgca agatacatgt actctggaat gcttagataa atgctaaaaa
    18481 gcggtctatc ctagctctgt ccttttacct gagagtttga gcagttgcct gccttgcccc
    18541 ttcggtgcct ttacggtctc tccagagttc cgtccattta cagtcatgga aaatcaaacg
    18601 attccccact tctattaaac ttcattcggt gttggtattt aattgattct tattttacaa
    18661 aaaatgttgg cttttgtcaa tgtgtttatt agtaaaaatt agttcaacag tttttacttt
    18721 ataaagtcca gaatactgct atcctttaaa agtgacaata gtcgcaccac tgcctccagc
    18781 attttgtggg gcatagccga aactcttgac atgtttgttt ctttgcaagt atttggtaac
    18841 tccttcacgg atgactcctg ttccgatacc atggatgata tcaacttgag ccatattgtt
    18901 aagcaaggct tggtcgatga aggtatctag ctcattcatg gcttcttcat agcgcttgcc
    18961 tcgaagatcc agtctagctt gaggtcctcg cccagaagtt cgtttcacaa cattgacctg
    19021 tttcttcttg actggttttt cttgctgggc ttgaacaaga tcaaactctt tctcttccaa
    19081 ggtcatctta atcaagccaa cttgggcttc ccagcgaccg tccttgagtt gactggtcaa
    19141 ggtaccacgc tgaccataac tgagaaccac gatatcatct cccacctttg gagctcgttt
    19201 tttcttggcc ttttgaagga ccttattttt agacaagtcc actttttcag gagccaattt
    19261 tttcaacttg gccttggctt caatgatttc gtggggcttg agttgggatt tactgtggag
    19321 atttttgaga atctggtcac tttcacttag ggccatatcc acaatctcag cagcctgttc
    19381 acgcgccttg ttaagctcgg tttccttttc acgattaagc tcgttgtaga gtttttttag
    19441 cgcacggttc atcttgagat tttcttgctc cacctcacgg atattgtcca aacgtttgcg
    19501 gctttccagc gtctgctctt ctaattgctc aatgatacga ttgacgtcat tgtcctgatc
    19561 gatctgctga ctggcatctc ctacgataac ttcagatagg cctagacgtt tggcaatttc
    19621 aaaggcatta cttcggccag gaacaccctg cataaagcga taggtcgggc gaagagttgc
    19681 agtatcaaac tccatactgg cattttgcac aaaggctgtc tcaataccgt aggccttgag
    19741 ttctggatag tgggtcgtcg ccatggtctt gatttgacgc aggcgaaggt cctccagaat
    19801 agccatggca agggctgctc cctcttgggg atcagtacca gcccccaact catccaaaag
    19861 taagagtgaa tgttggttga ccttgccaag aatatccacg atattggtca tatggctaga
    19921 gaaggtagac aagctctgct caatagactg ctcatctcca atatcagcaa agatttcttc
    19981 aaaaatacca acacgacttc ccttgtctgc taaaatcggc aatcctgact gggccatgac
    20041 ctgtgtcaag cccagagttt tgagcatgat ggtcttccca cctgtattgg gacctgtaat
    20101 gacaatagct gttaaatctt gaccaaaata gacatcattt gcgacggcat ttttgaccaa
    20161 aggatggcag acatggagca gttgaatctc ttgattttct gacagctgag gcacgactgc
    20221 ttgtctttct tggataaatc gaaccttggc acgaatcaag tccagatgac cgataatcca
    20281 agcgtcatta gcaatctcag ccgcatgagg gcggacacgc tcagaaattt cttggagaat
    20341 gcgaagcatt tcatagcgct catctgctcg cagactagca atttcttcgc tcagtttgac
    20401 tacctcacgt ggttcgatat agacggtgtt tccactagca gaaatatcat gaacgacacc
    20461 tgcaatctta ttgcggtagg tgtttttgac tggtaaaacc tgacggccat ttctgctagc
    20521 aacaattcct tctgtcaaca tctgcgcttt ttgcttgagc aagtcttgta aaacatcgcg
    20581 tacctgactc tcgctatcat gtatttttcg acggattcgc gccaattctt cactggcaaa
    20641 attttcaatg aaacccgcat cattaaaggc ctgaagattt ccttgtaatt gcggaaaatc
    20701 atgtaatttc tcaaaccaaa gggctaattc ttccaagctg acattttcca gattggcgta
    20761 aaaactttga agttctcggc tggcaagaag cacgcgtttc aagagtagga actcctcgat
    20821 attgagatcc gctcccatct ccaacctctt gcagactcct gcaatttcct tagttgagag
    20881 aatagtaaaa tgcggttgct cgacgaaaag agcctgcatt tccttcatct cagcaaaagc
    20941 ctgtttgatt ttatctgctt tggcagtcgg agccagttgt ctcaattgct ccaagccctg
    21001 ctcggtcaac aaatgaggct caaacaaggc cttgacctta tcgaactcta atgtttctaa
    21061 tattttctta ttcatcttta tttccttgtc tttgaatgtc taggtgtggt agctttttac
    21121 attctgatag attcgagtca aatctgtaaa caaagggcta ggaaaactcc tgcccttttt
    21181 atccgattaa atttgtcacc cagatttgtt tgagccaact ggttgttatc ggtatgctct
    21241 ggatgatgtg ttttgcgacg atactctttt caagaggatt ttgtataact gccatgggga
    21301 tggtcgccaa gattgtcaag gccatttgca agacaaataa ggtcaccaac atggacaaga
    21361 tacctgctga aacttggaac aacttaccac ccagtttttt gctaggaatc aagtgtaaga
    21421 gaagaccaag caaacgacca atgctataga caatcccaaa tacaagcaag tagccgatgc
    21481 ctgcataaaa gaccttatcc agctgaaaga gttgatccga tgggaaaaag aaagtcccct
    21541 gaccttcctg cgaatttgca taagggagca ataaatggaa ttgctctcca agccccttat
    21601 aaaactggcc agccataaaa gccgatgcca tggctgaaat caggtaataa acctgtaaga
    21661 gcaggcctct ccgatagccg atataaaatc cccaaaccaa gaccaataga agaaggaatg
    21721 aaatcataag gaatcctcaa tcttgctctg ttcttgctta caagtcacaa gcttgtgacg
    21781 gagttcttct agctcttgct ccttatcgtc aaattcaatc tcatggctga gctgagttga
    21841 taaacagttg actgccaaca aaagagcgat tgtttcatca tctgcgctag gcatttgttc
    21901 tttaattgct tggtattttt ctgtcgcaac cttagcgatt tcctccataa aaaggttatc
    21961 atgttcgctt gtcaaggtta acgatttttt cccgaatgta aatttgaatc gatttagatt
    22021 tgccataaaa ttcacctcac gatattatac caaaattcgc taattttgtc agtttttaca
    22081 aattttactg cttttgtggt acaatagaaa ctatggcaag tatcacactc acaccaagcg
    22141 aaaaggatat tcaggctttt cttgaacact atcaaaccag tctggctcct agcaagaatc
    22201 cctatatccg ctactttttg aaactacctc aagcaacggt ttctatctat acttctggaa
    22261 aaatcttgct tcagggtgaa ggggctgaaa aatacgccag tttctttggc tatcaagctg
    22321 tagagcaaac cagcggacaa aatcttcctt taattgggac agatgaggtg ggaaatggtt
    22381 cctactttgg tgggcttgca gttgtggctg cctttgtcac acctgaccag cacgactttt
    22441 tacgaaaact cggtgtgggg gattctaaga ctctgaccga ccaaaagatc cgtcagattg
    22501 ctcctattct caaggaaaaa attcagcacc aggcactcct tctctcaccc agcaagtaca
    22561 acgaggtcat cggagaccgc tacaatgctg tttcggttaa ggttgccctc cataatcagg
    22621 ctatctatct cctccttcaa aaaggtgttc agcctgagaa aattgtgatt gatgccttta
    22681 ccagtgctaa aaattatgac aagtacttgg cacaagaggc caatcgtttc agcaatccta
    22741 tcagcttaga agaaaaggct gagggcaaat acttggctgt cgcagtttct tctgtcattg
    22801 cgcgtgatct ctttctggaa aatcttgaaa atctgggacg agaactgggt tatcagctcc
    22861 caagtggagc tggaacagct tctgacaagg tggctagcca gattttgcaa gcctatggta
    22921 tgcagggact cagcttctgc gccaaattgc actttaaaaa cactgaaaaa gcgaaaaaac
    22981 gcttagaaag gtaagttatg aattcattta aaaatttctt aaaagagtgg ggattgttcc
    23041 tcctaattct gtcattacta gctttaagtc gtatcttttt ttggagcaat gttcgcgtag
    23101 aaggacattc catggatccg accctagcgg atggcgaaat tctcttcgtt gtaaaacacc
    23161 ttcctattga ccgttttgat atcgtggtgg cccatgagga agatggcaat aaggacatcg
    23221 tcaagcgcgt gattggaatg cctggcgaca ccattcgtta cgaaaatgat aaactctaca
    23281 tcaatgacaa agaaacggac gagccttatc tagcagacta tatcaaacgc ttcaaggatg
    23341 acaaactcca aagcacttac tcaggcaagg gctttgaagg aaataaagga actttcttta
    23401 gaagtatcgc tcaaaaagcc caagccttca cagttgatgt caactacaac accaacttta
    23461 gctttactgt tccagaagga gaataccttc tcctcggaga tgaccgcttg gtttcgagcg
    23521 acagccgcca cgtaggtacc ttcaaagcaa aagatatcac aggggaagct aaattccgct
    23581 tctggccaat cacccgtatc ggaacatttt aagaaaccta agaggccgag aatcaccaat
    23641 ctcagcctct tcttctatcg tgagaagtga ttgtttattg acaaatactc aatgaaaatc
    23701 aaagaacaaa ctaggaagct agccgtaggc tgcttaaaac agtgttttga ggttgcagat
    23761 ggaagctgac gtggtttgaa gatattttcg aagagtataa aataaaaaca atcccaactc
    23821 tcacctattc atgcaaagga attttatgga agtttatttt tcaggaacca ttgaacggat
    23881 tatttttgaa aatcccagca atttttatcg catcctcctc ctagaaatcg acgatacgga
    23941 cgcagaggat tttgatgact ttgaaatcat tgtcacagga accatggctg atgtaattga
    24001 gggcgaagac tatacttttt gggggcaaat tgtccagcac tccaagtatg gagaacaact
    24061 gcaaatcagt cgttatgatc gcgcaaaacc aactagtaag ggcttggtca agtacttttc
    24121 aagtagccat ttcaagggga ttggtctcaa gacagctcag aaaatcgtgg atacctatgg
    24181 cgaaaatacc attgacgaaa ttttgcaaca cccagaaaag ttagaaggca tcgcaggact
    24241 ctctgccaaa aatcgcgagg ctttcgtctc cactctccgt ctcaactacg gtacggagat
    24301 ggttttggcc aaactagcca actacggcat tcccaacaaa ctagcctttc agattcaaga
    24361 cttttacaag gaagaaaccc ttgatgtggt tgaaaattat ccctaccaac tggttgagga
    24421 tatcaagggt ttgggcttta ccattgctga ccaactagct gaggaactag gcatcgaaag
    24481 tcaggctcct gaacgcttcc gcgccggtct agttcacagt ctttttcagg cctgtatgga
    24541 aacaggagac acctatgttg aagcacggga tttgctggaa caaaccctta ctctccttga
    24601 gtcttcccgt cccgtggaac tagaccccag ccaagttgcc caagagctct cctacctgat
    24661 cgaagaagac aaggttcagc agattgatac caagatcttt gacaacagcc tctttttcgc
    24721 tgaggaaggc atccgcagtc acttggttcg tatccttgaa aaaggaaaac agaagagtca
    24781 tgatttaaaa accattcaaa aacatatcac tactatcgag aaagagcttg gaattgagta
    24841 tgataacatt caaaaacagg ctatctgtga tgctatccag aataaggtct ttatcctgac
    24901 aggtggacct ggtactggta agacaactgt tatcaatggg atcatcgctg tttacgccct
    24961 gttagaggga cttgacctca ggaagaaaag caacctgccc attcttcttg ctgctccaac
    25021 tggtcgagct gctcgtcgca tgaatgaatt gacaggtttg cctagcgcga ccatacaccg
    25081 ccacttggga atgacaggtg acgatgatac cagtcatctg gaagattacc tagatgctga
    25141 ctttatcatc gtggatgaat tctctatggt ggatacttgg ctggccaatc aactcttctc
    25201 caatatctct tctaacagta agatcctcat cgtaggtgac agtgatcagc taccttctgt
    25261 cagtcctgga caggttctag cggatctgct tcatattcct ttgattcctc agactcgctt
    25321 ggaaaaaatt tatcgacaaa gcgaagaatc aaccatcgtc accctagcta gtcagattcg
    25381 acagggcatc ttgccagctg atttcaccca aaaaaaagct gaccgttcct actttgaaat
    25441 tgctagtggc catattcctg ccaccattga aaaaatctta ggtgccgccc tcagaaatgg
    25501 tattcctgcc cgtgatatcc aagttctggc tcctatgtac cgagggacgg cagggattga
    25561 tgctatcaat cagctcatgc aagacctgct caatccccca caaaaagatc aactcagttt
    25621 tgaagctcct cagtgccact atcgtaaggg agacaaggtc attcatttgg ttaacgatgc
    25681 tgaaatcaat gtctttaatg gggatttagg agctatcaca gacctgattc ctggtaaata
    25741 caccgaatcg aaacaagacg agattgtcat tgattttgat ggtaatgaag tctcttaccc
    25801 ccgtaacgaa tggtacaaga ttcgcttggc ctatgccatg agtattcata agtctcaggg
    25861 aagtgagttc ccagttgtca tcctaccgat tactagtgct agccgacgta tgctggagcg
    25921 aaatctcatc tacacagcca ttacacgtgc caaaagcaaa ctcatcttac taggcgaatt
    25981 acaagccttc gactatgcta cccaacacat cggaactgcc cgaaaaacct atctgattga
    26041 acgcttcagt gatctactgg agaatgttga agaaaagcaa caagctgtct ctgaaacagt
    26101 cacatcaagt gcctctgaac aatcctacat cctaaccgaa gaaaactggg acagcattcc
    26161 agccatgatt gggattacag acacagacct caaagagatt tttggaaaat agtgcatcag
    26221 aaaaaccacc aattcaggtg ggttttctgt ttattaagat tattttactg ttgctgtgct
    26281 tgtgatcaat tcaacagctt ttttgatagt gatatcatgt ttcaacatgt cagctgaaag
    26341 caagttttga acttgtgcaa cttccatgtt gtagtctgct gccaattgct caacttcttt
    26401 ttggatttct tcttctgaag catcaaatcc ttcagctttg gcaactgctt cgataacaag
    26461 gttagtctta gtacgtgact cagcttctgc ttggtattgg ttgtgaaggt cttcttgagt
    26521 agttccagtg atttggaagt acatgtcagg gttgatccct tgacgttgca aattcccaag
    26581 gaattcattt actgaacggt gaacttcttc atggatcatt tcttctggaa gttctacgat
    26641 ttcagcattt tctacagctg tatcaattgc tgcaccttca actgcatctt tgtaagcttc
    26701 ttctttagca gcagccaatt ctttgcggta tttttctttc aagtcagcaa gtgtttcaac
    26761 ttcttcatca atgtcttttg caagttcatc gtcaagagct ggaacttctt tagcttttac
    26821 ttcgtggata gttgtcacga atttagcttc tttacctgca aggtcttctg cttggtagtc
    26881 ttctgggaat gttacgataa catcaacggt ttcgccagct gagtgaccta ccaattggtc
    26941 ttcgaaacca gggatgaatt gacctgaacc aagtccaagt gagaagtttt caccttttcc
    27001 accgtcaaat tcaacaccgt cgatagaacc aacgaagtcg atcacaacag tgtcgccgtt
    27061 ttcagcagca gcttccttga taaccaattc agccaggttg ttgcgttcgc gttcgatacg
    27121 ctcttcgaca tcagcgtcag ttacttcttt ttctacatca actgatactt caaggttttt
    27181 gtagtcaccc aattttactt caggttttgt aacgacttca gcagtgataa cccagtcttg
    27241 acctttttcc attgaagtta cgtcaatttt tggttgggca accacttcaa gaccagcttc
    27301 ttttacagct gcttcataag cgtttggcaa aagagcgttc ataacgtctt ggtaaagtga
    27361 ttcttcacca aatttttggt cgaagatagg acgtggaagg tgacctttac ggaaacctgg
    27421 aacattaaga gatttcttca ctgacttgaa gacacggtcc aattctggtt tgatttggtc
    27481 ttgagagata gtgaaagtca agacaccacg gtttgtttct ttgttttcaa atgatacaga
    27541 cattctgtca tttctcctta aaatttttta aatacagtct attataacat tttctataaa
    27601 taaaatctag actaatgcag ccaattatag acaacaagcc tttttcctaa ctaaaacact
    27661 cataaagtat agacaatttt agactacttt ggatatcttt tggttagtca ttttccaaaa
    27721 ctaatttatc taatttttca gttatatagt agattg
//
LOCUS       DAVHEX010000024        27616 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVHEX010000024 DAVHEX010000000
VERSION     DAVHEX010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27616
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.024"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(9..731)
                     /locus_tag="VVL45_001024"
     CDS             complement(9..731)
                     /locus_tag="VVL45_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEU4187215.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNDGQLAHVLLSPKRHVDKTYLAQVKGIMTQEDVETFAEGIPLKDFTC
                     QPAILELVSIDTEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
     gene            complement(904..978)
                     /locus_tag="VVL45_001025"
                     /pseudo
     CDS             complement(904..978)
                     /locus_tag="VVL45_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(999..1229)
                     /locus_tag="VVL45_001026"
     CDS             complement(999..1229)
                     /locus_tag="VVL45_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU4187216.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
     gene            complement(1239..2465)
                     /locus_tag="VVL45_001027"
     CDS             complement(1239..2465)
                     /locus_tag="VVL45_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEU4187217.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEEFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALVPDPSPISQSGITFFNT
                     LFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMDIDGIR
                     EDGTRVPLFRNGNWAN"
     gene            complement(2487..2639)
                     /locus_tag="VVL45_001028"
     CDS             complement(2487..2639)
                     /locus_tag="VVL45_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187218.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            complement(2660..2938)
                     /locus_tag="VVL45_001029"
     CDS             complement(2660..2938)
                     /locus_tag="VVL45_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HEU4187219.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARN
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            complement(2931..3194)
                     /locus_tag="VVL45_001030"
     CDS             complement(2931..3194)
                     /locus_tag="VVL45_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEU4187220.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQALTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            complement(3296..7687)
                     /locus_tag="VVL45_001031"
     CDS             complement(3296..7687)
                     /locus_tag="VVL45_001031"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEU4187221.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPAFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHDLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFESVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYCSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            complement(7791..9872)
                     /gene="fusA"
                     /locus_tag="VVL45_001032"
     CDS             complement(7791..9872)
                     /gene="fusA"
                     /locus_tag="VVL45_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU4187222.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEEIRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     AKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            complement(10297..10767)
                     /gene="rpsG"
                     /locus_tag="VVL45_001033"
     CDS             complement(10297..10767)
                     /gene="rpsG"
                     /locus_tag="VVL45_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEU4187223.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            complement(10787..11200)
                     /gene="rpsL"
                     /locus_tag="VVL45_001034"
     CDS             complement(10787..11200)
                     /gene="rpsL"
                     /locus_tag="VVL45_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEU4187224.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            11617..15459
                     /gene="spuA"
                     /locus_tag="VVL45_001035"
     CDS             11617..15459
                     /gene="spuA"
                     /locus_tag="VVL45_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HEU4187225.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPGNANASLEKTEEKPAASPA
                     DPAPQTGQDRSSEPTTSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDD
                     VEKPSENWPNGALSFKDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVE
                     KLVPKMNEAWLDQDYKVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQW
                     PDGTDFTATGKYGRYIDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQ
                     IFLKDDDESIYTNPYYVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHL
                     GNKVTITDVAIDEAGKKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGK
                     LGADLKEEGKQVDLTLWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDST
                     NKLGITDFTGYYYQYQIERQGKTVLALDPYAKSLAAWNSDDSKIDDAHKVAKAAFVDP
                     AKLGPQDLTYGKIHNFKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDY
                     LKDLGVTHIQLLPVLSYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSD
                     PKNPEKRIAEFKNLINEIHKRGMGAILDVVYNHTAKVDLFEDLEPNYYHFMDADGTPR
                     TSFGGGRLGTTHHMTKRLLIDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARAL
                     NPNLIMLGEGWRTYAGDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPA
                     FITGGKRDVNTIFKNLIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKA
                     ENYAEIHRRLRLGNLMVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHL
                     LRDKDGNPFDYPYFIHDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQS
                     TDAFRLKSLQDIKDRVHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKARE
                     FNLGTAFAHLRNAEVLADENQAGPVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTS
                     HESTAEEKPDSTPSKPEHQNEASHPAHQDPAPEARPDSTKPDAKVADAENKPSQATAD
                     SQAEQPAQEAQASSVKEAVRNESVENSSKENIPATPDKQAELPNTGIKNENKLLFAGI
                     SLLALLGLGFLLKNKKEN"
     gene            complement(15559..16608)
                     /locus_tag="VVL45_001036"
     CDS             complement(15559..16608)
                     /locus_tag="VVL45_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU4187226.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRSEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(16727..18535)
                     /gene="glmS"
                     /locus_tag="VVL45_001037"
     CDS             complement(16727..18535)
                     /gene="glmS"
                     /locus_tag="VVL45_001037"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEU4187227.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEEGLSVLEAFKKALHIIRGSYAFAL
                     VDSQDPEVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNRRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDEAGQ
                     VVVDPDIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEVKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            complement(18728..20107)
                     /gene="bglA"
                     /locus_tag="VVL45_001038"
     CDS             complement(18728..20107)
                     /gene="bglA"
                     /locus_tag="VVL45_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEU4187228.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTAFRTSIQWSRIFPQGCGKVNTQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPESPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KELSDNNGF"
     gene            complement(20206..22059)
                     /locus_tag="VVL45_001039"
     CDS             complement(20206..22059)
                     /locus_tag="VVL45_001039"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEU4187229.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDDYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            complement(22072..23331)
                     /gene="rseP"
                     /locus_tag="VVL45_001040"
     CDS             complement(22072..23331)
                     /gene="rseP"
                     /locus_tag="VVL45_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEU4187230.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PEMAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPKDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            complement(23353..24156)
                     /locus_tag="VVL45_001041"
     CDS             complement(23353..24156)
                     /locus_tag="VVL45_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEU4187231.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIILMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            complement(24165..24923)
                     /locus_tag="VVL45_001042"
     CDS             complement(24165..24923)
                     /locus_tag="VVL45_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEU4187232.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            complement(25154..25726)
                     /locus_tag="VVL45_001043"
     CDS             complement(25154..25726)
                     /locus_tag="VVL45_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEU4187233.1"
                     /translation="MNTVKNKQEILEAFRESPDMMAILTIIRDLGLKDSWLAAGSVRN
                     FIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLEKKLREDFPQYQWELKNQVYMHQ
                     HSPHTASYTSSRDAMSKYPERCTAVGLRLNEELDFELYVPYGLEDILNFQVRPTPHFL
                     ENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            complement(25707..26678)
                     /gene="ruvB"
                     /locus_tag="VVL45_001044"
     CDS             complement(25707..26678)
                     /gene="ruvB"
                     /locus_tag="VVL45_001044"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEU4187234.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNDLEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDVITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            26840..26932
                     /locus_tag="VVL45_001045"
     CDS             26840..26932
                     /locus_tag="VVL45_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEU4187235.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
BASE COUNT         8064 a         6531 c         5237 g         7784 t
ORIGIN      
        1 aaactaaatt aaataatatt agcgcggaga atttctaatt cttccttggt caagcgacgc
       61 cattcccctc gttctaggtt ctcatctaat actaaagttc ccatagtcaa tcgttgcaag
      121 tccaccactt ccttgccaca gtagcccacc atacgcttga tctgatgaaa cttcccttct
      181 gcaatggtca cacggatttg gctttgattc ttttctgtat ctatggatac aagctccagt
      241 atagcgggtt gacaggtaaa gtctttgaga ggaataccct cagcaaatgt ctccacatct
      301 tcttgggtca tgattccctt gacttgtgcc agataagtct tgtccacatg acgcttgggc
      361 gaaagaagaa catgagccag ctgaccatca ttggtcaaga gcaaaagacc atgcgtgtca
      421 atatccaagc gtcctactgg gaaaacttcc ttactccgcg ccaagtcatc caacaagtcc
      481 agaacggttc tgtgcttggg atcctcagtc gctgagataa ctcctttggg cttgttcatc
      541 atgtagtaga caaactcttc atactccaac acttgcccat caaagcgaat ctcatctatt
      601 ttttcatcaa tctgcaattt agctgatttt tcttttttac catttacagt cacgcgccca
      661 gccttgagca agtttttgac ctcagtccga cttcccaccg cacaggcaac taaaaattta
      721 tctaatctca tagaactatt atatcatatc aaaaggaggc tagtacaatg accaacctcc
      781 ttttcgtttc atactcttca aaaatctctt caaaccgcgt caacgtcgcc ttgccgtata
      841 tatgttactg acttcgtcag ttctatctgc aacctcaaaa cagtgttttg agctgacttc
      901 gtcagttcta tctgcaacct caaagcagtg ctttgagcat cctgcggcta gtttcctagt
      961 ttgctctttg attttcattg agtatcagat ttaggaaatt aacttcctcg tctccaaaaa
     1021 atagctaaaa caatcatggc tcctaaaatc gctgggataa tagctgttcc tgataaaact
     1081 ggcccccaag ttccaaagag caagtgacct agaaaggctc cgatccaacc gagaaacatt
     1141 tttccaaaac atcccattcg ctctccacga ttggtcatag cacctgctaa aaatcccact
     1201 aggagaccaa cgaacatact tcctaacata ttatctcctt aatttgccca attcccatta
     1261 cggaaaagag gtacccgcgt tccatcctca cgaataccat cgatatccat ttggttagaa
     1321 ccaatcataa agtctacgtg aacatctgaa cggttaagcc ctgcagcttc aagctcctct
     1381 tcgctcatct ccgctccatc aacaacgcta gtcgcatagg ctgcaccgat agccaagtgg
     1441 tttgacgcat tttcatcgaa aagggtgtta aagaaggtaa tgcctgactg agaaattgga
     1501 cttggatctg gtaccaaggc acgcgcaccc gcattttcaa agacaaggtc tttcataacc
     1561 tgatcaccct tctcagcagt gatatctacg atttgtccat ccttaaaggt caccttaatg
     1621 ccttcaatga tatttccgtt gtagctaagc ggttttgtag aagtgacata accatctgca
     1681 cgacggaagt caggcgctgt gaagacctct tctgttggca tatttggcaa gaattcttcg
     1741 ccctgtgcat tgacagcacc agctgattcc caaacgtggt tctttggcaa accaagtgtt
     1801 aaatctgttc ctggcgctgt gtagtgaagg gctgaaaatt gctccttatt aagcatatcg
     1861 gccttgctct tgagaatggc tgcatgttcc tcccaagcct taacaggatc tgcttcgtag
     1921 acacggcaag ttttgaaaat ttggtcccaa aggaaatcaa ctgcttcttc gtcgctcgca
     1981 gcatttggga agactttctt agcccactca agtcctgctg cagctgctac agtccagcta
     2041 accttgttag attgagttgc gatacgcata ggcttcatgg caagtcccat agctttagca
     2101 gaagctgaaa gcttgtcagc gtccactccg ttcaaggcac ctggatcaga tgaacgaact
     2161 ccaagacggc tagccttatt ctccaagaga tagttcatct cagcaatctt gtattctggc
     2221 acattgtcca aacgctccat cggggcatgg aggaatttct cacggttaat cacatcatct
     2281 gtccactgaa cgatgacctc atgcgcaccc aaggcataag cttctttcac gattagatgt
     2341 gccaattcac gttgctccac atcaatagag agagccaaag tgtgaccagg ttgcacgtta
     2401 attccgttcg caaccaacaa tttcgcatat ttttctagat tttctttaaa atttggtaaa
     2461 accattctgt tttctctttt ctatttttat ttttctttca aagcagaaaa gattcctgct
     2521 aaagcaatag cacctgacaa gagtgctgtc gatagaagaa ttgtacgatc agcaagttct
     2581 aattgatatt caaatacctt cttagccggc ttatcttccg caaatattct tagtttcatg
     2641 agaaccccct taaaatagat taaaataaat cactgtgact gcctgtcctc agcaaattta
     2701 aaatcaattc cgacttgtct actttataaa ccaaaagcca atctggctgg gtatggcatt
     2761 cacgaactcc ttgaaaatgc ttggatgccg tcaatgaatg atcacgattt ctggcaggat
     2821 gttctttttc ttgaaccaga aaattcaaaa cttcttctaa taattctgcc ttcaaaccac
     2881 gcttcatagc caacttaaaa tcttttttaa actgtttatg ataacgaatc ttaagcacgt
     2941 aagtcctcca tcaaatctga gactgattca aaagactggc tcatattacg attttggtct
     3001 aaatccgtta acgcttggag caacttccga ttttcgtcta gtctaacatc aaaaggtaat
     3061 ccctgatatt gaattgcctg acgaaggaaa atattaatag ctgttgtcat atccattccc
     3121 agattactaa acacctgttg ggcctgctcc ttaacctcac tatccagacg gatgcttata
     3181 ctcatctttg acatactctc accctctttc catagactat tttaacaaaa aagaaagcta
     3241 atgtaaatct attggatata cgttagcctc ttttaataga ttattaagca attttttaaa
     3301 acaactcatc aaacaaactc aactggttat cctctggcat atttccaaga atacccatct
     3361 catccatctt ttcaaccaag gttgatgaga gtccaccacg cttgcgtagt tctgttttag
     3421 agaggaattc tccctcttca cgcgcccgca ccagttgctt ggcaacgttc tctcccagac
     3481 catccattgc tacaaatggt gggataaggg tatccccgtc gatgaggaac tctgtcgcct
     3541 gactacagta gagatctaat ttaccaaact tgaaacctcg ttcccacatc tcattgacaa
     3601 tctcaagagt tgtatagaga tcgatttcca cattagaggc ttcattgttc ttccgttttt
     3661 cagagatttc ttccattctg cgcttgatga cctccaagcc cgcacccatg gtcttgatat
     3721 caaaagcctt agcacgaatg gagaagtaag cacagtagta ataaatagga tggtgaacct
     3781 tgaagtaagc tacacgcaag gccatcataa cgtaggctgc cgcatgggcc ttagggaaca
     3841 tgtacttaat tttcccacag gattcgatat accactctgg caccttatta gccttcattg
     3901 cttcgatata gccatttctc tcctcttctg aaatctttag ccacaaaccc ttacgtaccc
     3961 gttccataat ggtaaaggcc atcttaggtt ccagacccgc atgcatgagg taaaccatga
     4021 tgtcgtcccg acaaccgata acagtcgata ggtccgctat tccttgctta atcagatcct
     4081 gagcattccc caaccaaaca tcagtaccgt gggacagacc agacagctga agcaattccg
     4141 caaaggttgt cggatgggtt tcgtctacca ttccacgtac gaaatttgtt ccaaactctg
     4201 gaatccccaa catacccgta ggcgttccaa tttgttcagg tgttacccct agcacatcag
     4261 tcccagaaaa gagtgccatc acgccttcgt catccatagg aattttatta gggtcaatac
     4321 cagacaaatc ctgaagtttt cgaatcatag tcggatcatc atgtcccagt acatcgagtt
     4381 tgaggacgtt ctcatcgata tcgtggaagt taaagtgagt ggtctgccat tcagccgtga
     4441 catcatctgc tggatactgg acaggcgtaa aatcgtagac atccatgtag ttcggaataa
     4501 caacgattcc ccccgggtgt tggcctgttg tccgcttgac acccgccgct ccttgagcga
     4561 ggcgttctac ttctgcatca cgataaaact tgccataatc tcgctcgtaa cctttgacaa
     4621 atccataggc agtcttggca gctaccgtac caaccgttcc cgcacggaag gcatattctt
     4681 caccaaagat atcacgcaca tccaagtggg cgctaggctg atcttctccc gagaagttca
     4741 agtcaatatc aggaacctta tccccatcaa aaccaaggaa ggtctcaaac ggaatatcct
     4801 gtccgttttt actgagtttg tgaccacagt ttggacagtc cttatggggc atatcaaatc
     4861 ctgaaccgta cgaaccatct gtgataaact cactgtactg acactgacca cagacatagt
     4921 gaggagagag aggattgacc tccgtaatcc caatcatggt cgcaacgaaa ctagatccga
     4981 cagacccacg agaaccaacc aaataacccc gttcattaga acgttgcacc agcatctgcg
     5041 atgccagata aatcacagca aatccattcc ccagtatgga tgttaattct ttttcaatcc
     5101 gcaaatcaac aatatctggc agcggatttc cataaatctc aaaagctttc ttataggtca
     5161 actcagcaac tgtttcttca gccttgtcga tgaaaggcgt atacaagtca cccttaacga
     5221 cttcaacgga ttcaaatatt tctgccaagg cattggtgtt ttcaataacc agtttacgag
     5281 ccagttcctc tcccaaaaag gcaaattcat ccaacatctc attagtcgtt cgaaaatgag
     5341 cctttggaag tggtgctggt tgggcatgtt caccatgacc gatagttcga ttaatcatcg
     5401 caccctgtcc caaactacgg acgataattt cacgataaat ctcttcttcc ggttcgatat
     5461 agtgaacatt tcccgtagcc agaacaggct tgccaaggcg gtctccaacc tctatcaaac
     5521 tcttgataat ggtctggagt tcctccatat ccttgacctg ctctttggca atcaagggtg
     5581 catagatagc cggtggcatg acctcgataa aatcataata cttggccacc tcaaccgccg
     5641 catccacacc ttgagaaacg accacgtcaa aaacttcacc ctctgaacag gctgaaccta
     5701 aaatcaagcc ctctcgatgg gcatctagaa ccgttctcgg aatccgtggc actccttcaa
     5761 aatacttggt attagacaag gaaaccagct taaagatatt ttttagacct acctgattct
     5821 tgacatagat ggtcgcatgc ttgatccgag cttttttgta agaatctgga ctgattagat
     5881 caatgttgag tctagctaaa tcggtcacac catgtttttc tgctacctct ttgataaaga
     5941 tgaaaagcag acgaccagtc gcttccgcat cgtagttggc catgtggtga tgttccaagg
     6001 ccacaccaaa acgcttggtc aaaggcccca aaccatggcg tttatactca ggatagaggt
     6061 ttctagcaaa ctccagcgta tcaataactg gctgactaat ctttggaaga tcatgccgct
     6121 cataattagc attcataaag ccaacgtcaa aggtagcatt gtgggcaact aggaccgtat
     6181 ccttgcaaaa ttcttggaat tcttgcaaaa cttgttctag tggtttggca tttttgacat
     6241 gatcatctgt aattccagtt aactctgtag taaaggctga caagggatgc ccaggattga
     6301 taaattcatc aaattcagca ataacattcc ccttgtacat cttagaagcc gcaacctgaa
     6361 tcaagtcatt atagatagct gaaagtcccg tcgtttccac gtcaaagacc acgtaggttg
     6421 cttctgacaa gtccatctcc acttcgttat agacgatagg gacacggtcc tccacgatat
     6481 tggcttccat cccatagatc agctggattc ccgctttctt agccgcctta tagccatgtg
     6541 gaaaggactg gacattccca tggtccgtga tagcaaccgc cttgtgtccc cacttagcag
     6601 ctgttgcaac aatctcttcg acctctggca aagcatccat agtcgacatg ttagtatgag
     6661 catgaaactc aacccgacgc tcaccttctg gcatcaaatc cttccgctca tagtgaacaa
     6721 cttcctgcag atcctgtacg ttcatagtca aatcgcgtgt gaagttattc atctccacat
     6781 tccctcgaac tcggagccaa gaattcttct tgatgaggtc aaacttctgg gcctcttcct
     6841 cgtttttaac ccacttttgc atagaaaaac ttgaagtata gtccgtcatt ttaaagttga
     6901 ttaaaacacg acctgttcta gtcacttttt gctccacatc aaaaacaacc ccttcaaata
     6961 ccagacgatt ttcctctgtc gtcacttcga tcataggagt aatctccgcc ttatccagct
     7021 tgggtttagc tgcagctttt ttcgcttgaa aatcaaaggc tggtttctct tccgctggag
     7081 gaggtgccat ctgctccagt tgttccatag cacggagcgc ttcctcattg gcagcttgaa
     7141 caatctgctc attttcagca tgaaaggcct cttcctgctc ttgggtcagg acatcattct
     7201 tctcgacttg acagttaaaa gttggaaaac caaacttttc aagttgtttg gctaaattag
     7261 gaagatgatt cttcttaaaa tgttccttat caatcgcttc agatccttca ataaatagct
     7321 gattaccctc agcacgaact tgcaaatttt gataaaggga cttaaaacct tgactagcac
     7381 atggaccttc agagaaagcc tccctatagt aggactgcaa gagctgattt gaaaattctt
     7441 gagaccgagc cttaatttca aaaacagctt tattgcctgt cttagaaaat tcttcgctca
     7501 aacctttctt taattctaaa aagatttcaa tcggtaaaat attagaaaat acgaaatgaa
     7561 actcccatac cttactaatt ttatgaacca caactcgctc aatattggcc tgtgctaaag
     7621 caggagcctg tctcatttca gcaggcatcc ccaattgatt catcaaaatt tcaaaactat
     7681 ttgacattca ttttcctcac attattcttc tactatttta ccatatttag aggtattttc
     7741 taaagacaaa aggaagccac taagtgactt ccttctagag tgaggacgga ttagtcttca
     7801 cctttatttt tcttaataat ttcttcttgt actgacttag gtacatcttc gtagtggtca
     7861 aataccatca tgaatgtacc acgtccttga gatgcagaac gaagaactgt tgcgtaaccg
     7921 aacatttcag caagtggaac gtaagcacga acgatttggc tgttaccgtg tgcttccata
     7981 ccatctacac gtccacgacg agcagttacg tgacccataa catcaccaag gttttcttct
     8041 ggaacagtga ttgttacaag catcattggt tcaaggatag ctggttgtgc tgatttagca
     8101 gcttctttaa gggaaagtga agccgcaatc ttgaaggcag tttcagatga gtcgacatcg
     8161 tgatatgaac catcataaag cttagcttta acgtcaacca ttgggtaacc tgcaagaaca
     8221 ccgttagcca tagattctac caaacctttt tcaaccgctg ggataaattc acgaggaacc
     8281 acaccaccga cgattgcgtt ttcgaattcg aatcctttac cttcttcgtt tggagtaaat
     8341 tcaatccata catcaccgaa ttgaccttta ccaccagact gacgtttgaa gaatccacgt
     8401 gcttgagtag aagcgcggaa tgtttcacgg taagatactt gaggagcacc tacgttcgct
     8461 tcaactttga actcacgacg catacgatca acaaggacgt caaggtgaag ttcacccata
     8521 cctgagataa ctgtttcacc agtttcaacg tttgtttcaa cgcggaatgt tggatcttct
     8581 tcagccaatt tttgaagggc gatacccatc ttgtcttggt cagctttaga ttttggctca
     8641 accatcaatt ggataactgg ttctggaacg ttgattgact caaggatgat tttagctttt
     8701 tcatctgtca atgagtcacc agttgtagta tctttcaaac caacggcagc agcgatatca
     8761 cctgagtaaa cagtgtcgat ttcttgacgg ctgttagcgt gcatttgaag gatacgtccg
     8821 atacgttcac gtttaccttt agaagtattc aatacgtatg aacctgattg aagaacacct
     8881 gagtaaacac ggaagaatgt caaacgacct acgaatgggt cagtcatgat cttgaaggca
     8941 agagctgcaa atggctcttc gtcagatgct ggacgaattt cttcagcgtc tgtatctggg
     9001 ttaatacctt tgattgctgg gatgtcaagt gggcttggaa ggtagtcgat aaccgcatca
     9061 agcatcaatt gaacaccttt gtttttgaag gctgaaccac acaatactgg gaagaattca
     9121 acgttgatag tcgctttacg gataccagct ttcaattctt cgttagtgat ttcttcacct
     9181 tcgaggtatt tcatcatcaa ttcttcgtca gtttcagcaa ctgcttcaat caatttttca
     9241 cggtattctt gagcttggtc aaggtattca gctgggatgt cttcttcaag gatatccgta
     9301 ccaaggtcgt tagtatagat ttcagctttc atcttgatca agtcaatgat accacggaag
     9361 tcatcttcag aaccgattgg caattggatt gggtgtgcat ttgcttgaag acgatcgtga
     9421 agtgtgctta cagagtaaag gaagtcagca ccgattttgt ccattttgtt ggcaaatacg
     9481 atacgtggaa ctccgtactc agttgcttga cgccaaactg tttcagtttg aggctcaaca
     9541 cctgattgtg agtcaagaac ggtaaccgca ccatccaata cacgaagaga acgttgtact
     9601 tcgattgtga agtccacgtg tcctggtgtg tcgatgatgt ttacgcggtg gttgttccat
     9661 tgagctgtcg tcgcagcaga tgtgatcgtg ataccacgtt cttgctcttg ctccatccag
     9721 tccatttgtg acgcaccttc gtgagtttca ccgattttgt ggattttacc agtgtagtaa
     9781 agaatacgct cagtagttgt tgttttaccg gcatcgacgt gagccatgat accgatatta
     9841 cgagtttttt caagtgaaaa ttcgcgtgcc atgaggtttg tttctcctat ttatttttga
     9901 tttctattct attataacac gattttaata aaaacggata ggcaggacct acccgttctc
     9961 aatgttttca tgctattgtt ggtttcaact tacgagatgg taagctgagt tatagctaat
    10021 actaatcgat ttagctaatt tgaacccggg ctaaagttag ttagccgatg tgagctggaa
    10081 cgggatgctg cgtgaaaaag ataaaactcc ttgtattcgt cgaatactgc gtcagtttcc
    10141 tatttttacc ttgcatcctt accgttccta gcatcatgat ttagttgagc tcaagctaaa
    10201 agtctggaga aaaagatgaa tctcattggt tgcaaccgca acctgcttcg attccctatt
    10261 ttctctggga ctttcttaac gctttcgcat cctatcttac caacggaagt gtgcgaatgc
    10321 acggttagct tcagccatac ggtgagtatc ttcacgtttc ttaactgctg caccagtgtt
    10381 gttagcagca tccaagattt cttttgcaag acggtcttgc attgtgtgtt caccacgaag
    10441 acgagcgatt gttaccaacc aacgaagtcc aagtgttgta cgacgttctg gacgaacttc
    10501 aactgggact tggtagttag aaccaccaac acgacgtgca cgtacttcaa gtacaggcat
    10561 gatgttttcc atagctgttt caaatacttc aagtgcatcg ttgccagtag cttctttgat
    10621 ttgctcaaaa gcaccgtaaa cgattgaagc agcagtacca cgtttaccat caagcataac
    10681 gcggttgata agacgagtaa ctagttgtga attgtatagc ggatctggca atacgtcacg
    10741 ttttggagct ctatttttac gactcatttc tctttatccc ctttccttat gcttttggac
    10801 gtttagtacc gtatttagaa cggccttgtt tacgatcgtt aacacctgca gtatcaagtg
    10861 caccacggac gatatggtaa cgtacccctg gaaggtcttt tacacgtcca ccgcgaagaa
    10921 gcaccacgct gtgctcttgc aagttgtgtc cgatacctgg gatgtaggca gtaacttcga
    10981 taaggttgct caaacgtaca cgagcgaatt tacgaagggc tgagttaggt tttttaggtg
    11041 tcattgttcc aacacgagtt gcaacaccac gtttttgtgg tgaagaaacg tttgtttgaa
    11101 cttttttatg actattgtaa ccaacgttca aagctggtga tttagatttt tctacttttg
    11161 atttacgcgg tttgcgaacc aattggttaa ttgtaggcat ctacattctc ctgtgttttt
    11221 ttatttttgg tgatgataca cttggtgaca gatatcatct gtgtgtactt ttgcaacatt
    11281 tgtcagcacg tccctgtaca ctcttgagag accaaaagta aaaagtaccg tctattattg
    11341 taacaaaatt ttcccttgct tgtcaagata tttttctttt ttattggtaa tgttagagat
    11401 caatttctga ataaaaatag ctgagaacac atcatcttcc taataacacg gcttggctta
    11461 cgtgacattt ttatctccgt catttatact gatatataaa atagtacgga aaacttctca
    11521 aaagtaagaa tttctttcac aaatcaaaga aaacgcttgc atattgtttt actacatgct
    11581 atactgatat agaaatttat tgattggaga atcattatga gaaaaacacc atctcacact
    11641 gagaaaaaaa tggtctacag catccgttcc ctcaaaaatg gaactggttc tgtccttatc
    11701 ggcgcaagcc ttgtcttgct tgccatggct acaccaacta tctcatccga cgaaagtaca
    11761 ccaaccacta acgaacccaa caacagaaat acaaccaccc ttgcccaacc tcttactgat
    11821 acagcagctg gctctggtaa gaacgaaagt gatatttctt cacctggaaa tgcaaacgct
    11881 tccctagaga aaacagaaga aaaacctgct gcaagcccag ccgatccagc accacaaact
    11941 ggacaagatc gttcaagtga gccaactact tctactagtc cagtaacaac tgaaactaag
    12001 gcagaagagc ccatcgaaga taactacttc cgtatccatg tcaaaaaact tcctgaagaa
    12061 aacaaggatg ctcaaggact atggacttgg gacgatgttg aaaaaccatc tgaaaactgg
    12121 ccaaacggag ctttgtcctt caaggatgcc aagaaagatg actacggcta ttacctagat
    12181 gtcaaattaa agggagaaca agccaagaaa attagcttcc tcatcaacaa tacagctgga
    12241 aaaaatctaa ccggcgataa atctgtagaa aaactagttc caaaaatgaa cgaagcttgg
    12301 ttagaccaag attacaaggt tttctcttac gagccacagc ctgcaggaac tgttcgcgtc
    12361 aactactacc gcacagatgg caactatgac aagaaatctc tctggtactg gggagatgtg
    12421 aaaaatccaa gtagcgctca atggcctgac ggaacagact ttacggctac aggcaaatat
    12481 ggccgctata tcgacattcc tcttaatgaa gccgcaagag aatttggatt tttattacta
    12541 gatgagagca aacaaggaga cgacgtgaaa atccgtaaag aaaattataa gttcacagat
    12601 ttgaaaaatc atagccaaat tttcctaaaa gacgatgatg aatcgattta cacaaatcca
    12661 tactatgtcc atgatatccg tatgacagga gcccaacacg taggcacttc tagcattgaa
    12721 agtagctttt caacacttgt cggtgctaaa aaagaagata tcctcaaaca ctccaacatc
    12781 actaatcacc taggaaacaa ggtaactatt accgatgttg caatcgatga agctggtaag
    12841 aaagtgacct acagcggaga tttctctgac acaaaacatc cttatactgt tagctacaat
    12901 tccgaccaat tcactaccaa aacaagctgg cgcctgaaag atgagacata cagctatgat
    12961 ggcaaactgg gagctgacct aaaagaagaa ggaaaacaag ttgatttgac cctttggtca
    13021 ccaagtgctg ataaggtttc tgttgttgtc tacgacaaga atgaccctga caaagtagtt
    13081 ggaactgtcg ctcttgaaaa aggggaaaga ggaacttgga aacaaactct agacagcaca
    13141 aacaaactcg gaatcacaga tttcactggc tactattatc aataccaaat cgagcgtcaa
    13201 ggtaaaactg ttcttgcact cgatccttac gctaaatctc ttgctgcttg gaatagcgac
    13261 gattccaaga ttgacgatgc ccataaagtg gctaaagccg cctttgtaga tccagctaaa
    13321 ctcggacctc aagacttgac ttatggtaag attcacaatt tcaagactcg tgaagacgcc
    13381 gttatctacg aagctcatgt gcgtgatttc acttcagatc ctgccattgc aaaagacttg
    13441 accaaaccat ttgggacttt tgaagccttc attgaaaaac tagactatct caaagacttg
    13501 ggtgtaaccc atatccagct ccttccagtc ttgtcttact actttgtcaa tgaattgaaa
    13561 aaccatgaac gcttgtctga ctacgcttca agcaacagca actacaactg gggatatgac
    13621 cctcaaaact acttctcctt gactggtatg tactcaagcg atcctaagaa tccagaaaaa
    13681 cgaatcgcag aatttaaaaa cctcatcaac gaaatccaca aacgtggtat gggagctatc
    13741 ctagatgtcg tttataacca cacagccaaa gtcgatctct ttgaagattt ggaaccaaac
    13801 tactaccact ttatggatgc cgatggcaca cctcgaacta gctttggtgg tggacgcttg
    13861 gggacaaccc accatatgac caaacggctc ctaattgact ctatcaaata cctagttgat
    13921 acctacaaag tggatggctt ccgtttcgat atgatgggag accatgacgc cgcttctatc
    13981 gaagaagctt acaaggctgc acgcgccctc aatccaaacc tcatcatgct tggtgaaggt
    14041 tggagaacct atgccggtga tgaaaacatg cctactaaag ctgctgacca agattggatg
    14101 aaacataccg atactgtcgc tgtcttttca gatgacatcc gtaacaacct caaatctggt
    14161 tatccaaacg aaggtcaacc tgcctttatc acaggtggca agcgtgatgt caacaccatc
    14221 tttaaaaatc tcattgctca accaactaac tttgaagctg acagccctgg agatgtcatc
    14281 caatacatcg cagcccatga taacttgacc ctctttgaca tcattgccca gtctatcaaa
    14341 aaagacccaa gcaaggctga gaactatgct gaaatccacc gtcgtttacg acttggaaat
    14401 ctcatggtct tgacagctca aggaactcca tttatccact ccggtcagga atatggacgt
    14461 actaaacaat tccgtgaccc agcctacaag actccagtag cagaggataa ggttccaaac
    14521 aaatctcact tgttgcgtga taaggacggc aacccatttg actatcctta cttcatccat
    14581 gactcttacg attctagtga tgcagtcaac aagtttgact ggactaaggc tacagatggt
    14641 aaagcttatc ctgaaaatgt caagagccgt gactatatga aaggtttgat tgcccttcgt
    14701 caatctacag atgccttccg acttaagagt cttcaagata tcaaagaccg tgtccacctc
    14761 atcactgtcc caggccaaaa tggtgtggaa aaagaggatg tagtgattgg ctaccaaatc
    14821 actgctccaa acggcgatat ctacgcagtc tttgtcaatg cggatgaaaa agctcgcgaa
    14881 tttaatttgg gaactgcctt tgcacatcta agaaatgcgg aagttttggc agatgaaaac
    14941 caagcaggac cagtcggaat tgccaacccg aaaggacttg aatggactga aaaaggcttg
    15001 aaattgaatg cccttacagc tactgttctt cgagtctctc aaaatggaac tagccatgag
    15061 tcaactgcag aagagaaacc agactcaacc ccttccaagc ctgaacatca aaatgaagct
    15121 tctcaccctg cacatcaaga cccagctcca gaagctagac ctgattctac taaaccagat
    15181 gccaaagtag ctgatgcgga aaataaacct agccaagcta cagctgattc acaagctgaa
    15241 caaccagcac aagaagcaca agcatcatct gtaaaagaag cggttcgaaa cgaatcggta
    15301 gaaaactcta gcaaggaaaa tatacctgca accccagata aacaagctga acttccaaat
    15361 acaggaatca aaaacgaaaa caaactccta tttgcaggaa tcagcctcct tgcgctcctt
    15421 ggtctcggtt tcttactaaa aaataaaaaa gagaactaaa ctagccctcc tatagaaaaa
    15481 tcccccaagc attatagctc gggggattaa tttttgtaca atatttgttg tcctaataaa
    15541 cttgattagg attttttatt aagcctcttt catagcaaaa taagctcgta ctttgggtgc
    15601 aacttgtgtt ccgaagagtt caatagctct cagaacctgg tcatgaggca tagaaccaag
    15661 cggtagatga agcatgaagc ggtccaatcc taaatcctct atcatgcgaa tcaatttttc
    15721 ggccacctga tctggattgc caacaaacat ggcgccattt ggccctacct gctccaaata
    15781 ttgctcataa cgcaattcct gccagtgcgg acggtctttg gaaatagcat ccaccacttg
    15841 cttagtcgga tggaaataat ctttcaccgc ctgctcacca tcttccgcaa tccaccccca
    15901 agaatgggct cccactttca agtctttgtc agcatggccc gcttcgcttc caatctcacg
    15961 ataagcctga atcaactttt taaaataacg tggattacca ccaataatag catatacaat
    16021 cggtagacca gcctgagcaa tcttcactgt tgattcgaca tgaccacctg tagctatcca
    16081 caagggcaat ttgtcctgaa ctggacgagg ataaacttct ttaccagcaa tcgtttgagt
    16141 caatcgacct tgccagtcta acttggtctt ttcattgact aactgaagca agtctaattt
    16201 ctcatcaaaa agagagtcgt agtctttcaa gtcataacca aacagaggga aagattccgt
    16261 gaaagagccc cttccagcca taatctccga tcgtccattt gacaaagcat cgatagtggc
    16321 atactgttgg aacaaacgaa tcgggtccat gcttgacaga atgctgactg cactggtcaa
    16381 acggattttc ttggtattga ctgccccagc ggccagaaca atctctgggg ctgatactgc
    16441 aaaatccgcc cgatggtgct caccaatccc atatacatcc aaaccaacct tgtcagccag
    16501 ctcaatctct gccaccaact ggcgaatgcg ttcagcatga ctgtaagttt gtccagtccc
    16561 ttcaagctcc gttatttccc caaatgttga aattcccaat tctaccattg tgattctcct
    16621 tatctatctc tgtacttcaa tttgaaaaat tattctaaca cgaatcttga gtacaagcaa
    16681 ccgatttgct cattagaaaa agcctagata actagacttt tttagcttat tctaccgtta
    16741 ctgacttggc aaggttacgt ggtttgtcca catcgaggcc acggtggagg gttgcaaagt
    16801 aagcgactaa ttgcgttggt acgaccattg aaattggtga gaggtatgga tgtacggtcg
    16861 taaggacgat atcgtcggta tctttggcta cattctcttc tgcgatagtg aggactttgg
    16921 caccacgggc tgcgacctct tggatatttc cacgagtatg attggcaaga actggatctg
    16981 acaagagagc caaaacaggc gttccttctt caatcaaggc aatggttccg tgcttgagtt
    17041 ctcctgcagc aaagccttca cactggatat aagaaatctc tttgagtttg agacttgctt
    17101 ccatggctac gtagtaatct tgaccacgtc cgatgtaaaa ggcgttacga gttgtttcaa
    17161 gaagttcacg aaccttgact tcaatggttt ctttctctga aagagttgat tcgatagact
    17221 gagctacgat tgacaattca tgaaccaggt caaaggcttg cgctttagca ttaccatttg
    17281 cttctccgac tgcttttgca aggaaggcaa gggctgcgat ttgcgctgta taggctttag
    17341 ttgatgccac ggcaatttca ggacctgcgt gaaggagcat ggtatagttg gcttcacgtg
    17401 agagggttga acctggaacg tttgtcactg ttaagcttgg aattcccatt tcattagcct
    17461 tgaccaaaac ttgacgacta tccgctgttt caccagattg gctgataaag atgaagagtg
    17521 gtttcttgct gagaagtggc ataccgtagc cccactcaga tgagattcca agttcaactg
    17581 gtgtatctgt caattcttcc aacattttct tagaagcaaa tcctgcatgg taagatgttc
    17641 cagctgcaag gatgtagatg cggtctgcgt cttgaacagc cttaatgata tctgggtcta
    17701 cgacaacttg accagcctca tctgtgtagg cttggatgag tttccgcata acagttggtt
    17761 gctcgtcaat ttccttgagc atgtagtaag ggtaagttcc cttaccgata tctgacaagt
    17821 caagttcagc agtgtagcta gcacgctcac gacgatttcc atcatagtct tgaacttcca
    17881 cactatcagc cttgacgatt accaactctt ggtcatggat ttccatgtat tggttagttt
    17941 cacgaatcat agccatggcg tctgagcaga ccatgttata gccttctcca agaccaatca
    18001 aaagtggtga tttattttta gctacgtaga tgacttcagg atcttgtgag tcaaccaagg
    18061 caaaggcata agaaccacgg atgatgtgaa gggctttttt gaaggcttca agaactgaga
    18121 gcccttcttc ttccgcaaat tttccaatca aatgaacggc tatttcagta tctgtctgcc
    18181 ccttgaagtg gtgacctgca aggtattctt ccttgatttc aagatagttc tcaatcaccc
    18241 cattatgcac caagacaaaa cgttccgtct cagagcggtg tgggtgagca ttgtcctcag
    18301 ttggttttcc gtgagtagcc caacgagtat gtccgatacc agttgttccc tcaacaccag
    18361 ctgtcttggc agacaattct gcaatacgac caaccgcctt caccaaatgg ttatcagcac
    18421 catctaggac aaaaattccc gcagaatcat agccacggta ttcaagcttt tcaagccctt
    18481 gaatcaaaat atcagttgca tttgtgtttc caacaacacc aacaattcca cacatagtat
    18541 atacgacaca ggcaagctgt gctttctcct taaaattggt atagtctaat tcatctttta
    18601 tagaatcagc aaaaacagta tatacttgtt tctttcactt gtcaagagta aaaattggta
    18661 tagttcaaat taagctcctg taagcataaa aactctgacc gattgggata atcagtcaga
    18721 gtccttttta aaatccatta ttatcgctta attctttgaa ccagtggcct gatttcttca
    18781 gacgacgttc ttgcgtttcc aagtctaatt cgaccaaacc atagcgattt ttatagctgt
    18841 tgagccatga ccagcagtca ataaaggtcc aaatcaagta gcccttacag ttggcaccat
    18901 cttcaatggc acggtgaagt tcacgaagat gaccttttac aaagtcaata cggtaatcat
    18961 cttgaatcat tccatcttga cggaattttt cttccccttc aacacccata ccattctcag
    19021 tcaacatcca ctcaatattg ccataatttt ccttgatatt ttgggcgatg tcataaatcc
    19081 cttgctcata aatctcccaa ccacggtgag aattgatttt acgtccaggc atcacataag
    19141 gctcgtaaaa atgttctggt aagagtggac tctctggatg cttagcaaat cgaggagcca
    19201 taacacgcaa aggttgatag tagttcacac caaggaagtc caccgtatta tcacgaatga
    19261 gttccaactc ttcctctgta gcatcaggta aaagaccgtg ttcatgcaag atttctacca
    19321 actcctgtgg ataagtcccc aagacagatg gatctaagaa agattgggcc tgaaaaaggg
    19381 ccgcaatacg agctgccttg acatcagcag gatgctggct acgtggataa gccggtgtca
    19441 agttgaggac aatcccaatc ttggaatcag gcaaaagttc atggcaagcc ttaacagccc
    19501 ggctgctggc caattgtgta tgataggcta ccttaacagc tgcctctgca tccaccttat
    19561 gtggataatg ggcatcataa aaataaccaa attctacagg aacgatgggc tcgttaaagg
    19621 taatccattg atccactaaa tctccataag tctcaaaaca aaaacgagca tagtcttcat
    19681 aggctgagac tgtcgcctta ttttcccaac catcaccatc ctcttgaagg gcaaaaggta
    19741 aatcaaaatg atagagattg actaacagac gaattccttt agccttaata gcctcaaaga
    19801 ccttacgata aaaatccaca ccttgagtgt tgacttttcc acagccttgt ggaaaaatcc
    19861 gtgaccactg aatagaagtc cgaaaggctg tgtgaccagt ctctaacaaa agctcaatat
    19921 cccgctccca attttcataa aaagtcgatg tcttatctga accaatccca ttatagtaac
    19981 gatttggctc cacttggaac cagtaatccc agagattgtc tcccttaccg tcaccagcta
    20041 cacgtccttc tgtctgcggt ccagaagtag aggatcccca gacaaaatcc tttggaaatc
    20101 ttagcataca tttacctctt tatctactca tttctcccat tatacagaaa aaacaaggta
    20161 aaaactagtt acattttttc cttgtttttc ttctgattat agtttttatt tcttgcttag
    20221 gatttcaagc gtttcaagca cgttatctgc atgaacctca atggtgtcac cagttgcctt
    20281 gatcttaact tctacaatgc catcggccgc ttttttacca acagtgatac ggattggaag
    20341 accaatcaag tcactatcgc taaatttaac accgacacgt tcgttacggt catctgtcaa
    20401 gacttcataa ccagctccca tcaagcttgc ttcaagtttt tctgtcaagg cttgcgcttc
    20461 ttcatccttg acattgacag taatcaaatg cacatcaaat ggtgccaatt ctttagggaa
    20521 attgattccc caagcgtaac ggtattcacc ttttggcgtt ttgttaacaa agaggcgagc
    20581 gtgttgctcc atcactgctg aaagaagacg gctgacaccg ataccgtaac atcccatgat
    20641 gattggcaca gcacgaccat tttcatccaa gacatctgct cccatgcttg ctgaatagcg
    20701 agttccgagt ttgaaaatat gaccgatctc aataccacgc gcaaagttaa ggacaccttg
    20761 tccatctggg gaaatttcac cctcacgaac ttcacggata tccacatatt ctgcagtaaa
    20821 atcacggcct gggttcacac cagtcaagtg gtagtcatct tcgttagcac cgacaactgc
    20881 attgcgaaca tcttgtacct tacgatctgc aataatttta atattctctg gcaaaccaac
    20941 tggtccaagt gaaccaaatc ctgcttgaac aacattcgcc acttcttctt cgctagcaac
    21001 gtcaaagaaa tctgctccca agtgattttt caacttgact tcgttgagtt ggtcatttcc
    21061 aactagaagg gctgcaacaa gctcaccatc tgcaatgtag aagagggttt taatcgtttg
    21121 ttcttctgga acattgagga aggctgcaac ttcatcaatt gatttaacat ctggcgttgc
    21181 aacacgagta acttcttctt cagcgacaac acggttgctt ggtttgtact cgtttgttgc
    21241 catttctaag ttagctgcat agctagactc acttgagtaa gcaatggtat cttcaccaga
    21301 gactatccat ttgagcaatt ctgccttgat ttcttcttgc acttctgcag gaatttcgtc
    21361 aaatgaggca actgacttgt ccaagacaac ccagcggtca aggtctgtac gagcagatgt
    21421 aatggccata aattcttggc tatccttacc acccatggct ccaccgtcac caataatagc
    21481 cttgaagtct aaaccactac gagtgaaaat acgctcatag gctgctttgt actcatcata
    21541 aacactatcc aaactatcat agttagcgtg gaaactataa gcatccttca tgataaactc
    21601 acgtgtacga agaagtccat tacgcgggcg tttttcatca cgatacttgg gctgaatttg
    21661 ataaaggttg agtggcaatt gcttgtaaga tttaacagaa tcacggacaa tagctgtaaa
    21721 ggtttcttcg tgagttggac ctaagataaa gtctgatttt tcacggtttt ttagtttgta
    21781 aaggtcttca ccataggttt cgtaacgacc tgattcacgc cacaattctg cactaagaag
    21841 ggctggagcc aacatctcaa cagcaccaat cttttcgaat tcttggcgca tgatgttttt
    21901 agctttttca atcacacggt tggcaagtgg tagataagaa taaacacctg ctgaaacttg
    21961 gcgaacataa ccagcacgca acataagagc atggctgata acttgagcat cgcttggcat
    22021 ttcgcgaagc gttgggatag gcattttact ttgtttcata atattcctcg attatctaaa
    22081 aaagagtcgc ataatgtcat tccaagtcac agcaatcatc aagacaacca tgatgaccac
    22141 tccggccaag gtgacatagg tttcaatttc ttgtttcaat ggtttgcggc ggatggcttc
    22201 taggatattg agcacaatct taccaccatc caaggctgga atcggaataa gattaaaaat
    22261 cccaatattg atggaaatca ttgccaagaa gtacaagata ttttcaattc catttttagc
    22321 agcatcacta cttgccttaa agatagcaac aggtccaccc aacttgttca aatctggttg
    22381 gaaaatcaga tttttcagag ctgagagaat tcggagagct gagtcagcag cagttgtaaa
    22441 accacctaca aacatggata gaaaatctga cttaaccccc ggttgaacac ctagaaggta
    22501 acgaccttga ctatctttgg gtgtaacagt gacttgtttg tcactcccct tttcagaaat
    22561 agtcacatcc aaagtcggtg ccgtcttatc tttggtttct gtttccacag cttggatcaa
    22621 gctttcccag ttgctaacct catgtgagcc aatcttggta atttgtgcca tttctggtac
    22681 tcctaccttg gccaaggcac cttggggcat gatatggaac tgattggtat caacatctct
    22741 gacaccaccc tgcataaaga ttaaaaccca aaaaacaacg acacctaaga taaaattgtt
    22801 cataggacct gcaaaattgg taatcagttt gccccagata gtcgcatttt gatattgaac
    22861 atctaaaggt gcaatccgaa cctcagtacc atctgcttcc acaaccgttg catcgtgatc
    22921 cactgcaaat gttttttctt cttccagaac caatcctttg ataaagagct tgtcttcaaa
    22981 atcaaactgg gtcacctgca tagggagggc tgtttgatcc aattttttac ctgagagatt
    23041 gatgcgttta accttaccat catcagcaag tgtcaaacta acaggcgttc ctgtcttgat
    23101 ttcagttgta tcatcacccc aaccggccat gcggacatag ccacccagag gcaagattcg
    23161 aatggtatag gccgttccat ccttgccaat gtgagcaaaa attttaggtc ccataccgat
    23221 ggcaaattca cgtactaaaa tccctgattt cttggcaaag tagaagtgac cgaactcgtg
    23281 caccactaca ataatcccga aaaccagaat aaaggttaaa attccgagca tagcgtttcc
    23341 tccgtctttt gattaaaaga gtccaaataa gtgcatgatt ggaaatacaa gcaacatact
    23401 atcgaaacga tccaaaacac caccatgtcc agggataaat ttcccagaat ccttaacacc
    23461 aaaatgacgt ttgatcgaac tttctagtaa atcaccaaat tgtccagcaa tgctaaagaa
    23521 aatagcaaag actgacatct tgtaaattcc atatggaaga gcaactgtac tgtcaactat
    23581 cataaggata atggttacta aaattgctcc taaaatacca cccaaggcac cctcaagggt
    23641 tttattaggc gatacccttg gtgctaactt tcgtttccca tagttcatcc caacaagata
    23701 ggcaccactg tctgtcgccc agacgataca caaggctaag agagccttgt ccaaacctgc
    23761 aacacgagca tctagtaaag cattaaatcc aaagcccacg tagaagctca tagcaagagg
    23821 gaaaaccgca tcctcaatcg tataagactt gctaaaaacg gtcgttccta acatgattga
    23881 aatcaaaaca ctataggcaa ccacattccc atcaactggc aaaaaagtca ggtaattctc
    23941 caagggaatg gtcaatgcaa aggttgcaaa gagggtcaag aggccctcca tcgtcatggt
    24001 ctctagacct ctcatcttca aaagttcatg catggctagc atggctatga ttccgattgc
    24061 tatctgaagc aagaggcccc caatcattaa aattggtagg aaaatagcca gggcaatccc
    24121 tgcaaacaag gttcttttct gtaaatcctg ggtcatattt cctcctaaac tcctccaaat
    24181 cggcgatgac gacgattata ggcaagaata gcttcctgca aggccgcttc gtcaaaatca
    24241 ggccataagg tgtccgtaaa ataaagctca ctataggctc cctgccatgg aaggaaattg
    24301 ctcaaacgta attctccact agtacggata atcaagtctg ggtctcgtaa gtccttaggc
    24361 aaatgctgag taaagagata gttaccaatc aattcctctg tgatgtcacc tgggttgatt
    24421 ttggcatcta aaacatcctg ggaaatcaac ttaagcgcct gtgtaatctc agcacgtcca
    24481 ccatagttaa gagcaaaatt aagaatcaat cctgtgttgt tcttagtcaa ttcctcagcc
    24541 ttggttaaag cttcaaaggt ttgcttaggc aggcggtctg tctccccaat catttgaatc
    24601 ttaacattat tcgcatgtag ttccgggaca taattatcat aaaactctac tggcaagttc
    24661 atgataaact tgacttcctg atctggacgg gtccagtttt ccgtagaaaa agcatagacc
    24721 gtaataacct tgacgcccag tttgttggct gccttggtca cggtttgcaa tgcttccatg
    24781 cccgccttat gtccaaaaac tcgcggttgc atacgttttt tagcccaacg gccattgcca
    24841 tccatgatga tgccgatatg agcaggaacc tgtgtcggaa cctctacttc cacagcctta
    24901 tctttcttaa aaaatccaaa catgatctta ttcctattca aaaatctatc gtttcattat
    24961 accatatttc cccattttct tctatcacta agctatttat tctcaggcac caagcccatt
    25021 tttcaaaaaa ataagccgcc tgattgggcg actttatttt tatagggaga ttattatgaa
    25081 aaagttttag gagtttaagt taaggtcttc ttaacttatg aacttagtgt acactcccta
    25141 gcttaaagtt tccttaagta tttttaaaaa tcaaattttt ccatttctcc tgccaatttt
    25201 tcttggataa acgtgtttga tagagttcca ttcggtcttc attttctaag aaatgaggag
    25261 ttggacgaac ttgaaaattc aaaatatcct ccaaaccata aggtacatag agttcaaaat
    25321 ctaattcttc attcaagcgc agtccaactg ccgtacaccg ttctggatac ttactcatag
    25381 catcacgaga actggtatag gaagcagtgt gaggactgtg ctgatgcata tagacctgat
    25441 ttttcaattc ccactggtac tgaggaaaat cctctctcag ctttttctcc agtaataagg
    25501 tttcctcata agaaaaatct ggatcaaaga aaatcacatc tatatctgtt tcatgatcaa
    25561 aaggggattt gtctgacaaa agattccaga tgaaatttct gacagaacct gctgccaacc
    25621 acgagtcttt caaaccaagg tctcggatga tcgtcagaat ggccatcata tctggacttt
    25681 ctctaaaagc ctctaagatt tcttgcttat ttttcactgt attcataacc taagtgctca
    25741 tatgccttag cagtcgccac ccgtccagac cgtgtccgca tgataaaacc tttttgaatc
    25801 aagtaaggct catacatgtc ttcaactgtc tcacgctctt cggcgatatt cacagaaaga
    25861 gttcctagac caacaggtcc tccactgtac atctcaatca tggtgcgaag gattttttga
    25921 tccacatagt ccaaaccttc atggtcaaca tccagcatag tcaaagcctt atcggtaata
    25981 acatcatcga taaccccatt ccccattatc tgggcaaaat cgcgcacgcg cttgaggaga
    26041 cgattggcaa tacgaggggt tccacgacta cgtagggcca actcagatgc tgcctcatgg
    26101 gtgatttcca tctcaaaaat atctgccgtc cgctcgacaa tttctgtcaa gtcagcatga
    26161 gcataatact ccatatgacc tgtaatccca aaacgtgccc gtagtggatt tgagagcata
    26221 ccagcccgag tcgtcgcacc aatcaaggta aaaggaggca actccaaatg aacactgcga
    26281 ctgccttcac cagccccaat cataatatcg atgtagaagt cctccatggc actataaagc
    26341 acttcttcca ctgacatggg taagcgatga atctcgtcaa taaagaggac atctccaggc
    26401 tctaaatcat tcaaaatcgc taccaaatca cccgcttttt cgataacagg accagacgtt
    26461 tgcttgagat tgactcccag ttcattggca atgacaaaag ccatggttgt tttcccaagc
    26521 cctggagggc caaataagag cacatgatcc agcgcttcat cccgcatttt agcggcttcg
    26581 ataaagatct gaagttgatc cttaacctta tcctgaccaa tatattcacg taaatactga
    26641 ggacggagcg tgcgttctac taactcctca tcacccatca tctcattatc taaaattcta
    26701 ctcatggctc tattatatca aaaaaaacaa gccacaaaca aaaaagccac ctgattgggt
    26761 gactcctaag tttagcactt atgtggtata atattatacg gcacttctac accgcctacg
    26821 aaaggaggtg agatagccca tgatggaatt agtactcaaa actattatcg gaccaattgt
    26881 ggtcggtgtc gttcttcgta tagtcgataa atggctaaac aaggacaaat agtgtcaaaa
    26941 aagacctcaa gcttatttgg tcgtgagctt ggggtctttt ctagcctatg atatagaact
    27001 agtactcaat tcctttttat tatcccatag ttcacgaatt ttgtcaaaac tttacatttt
    27061 cttcaaccgc tgtacgacaa gacggttaag attaagagaa cgttagggat tctatcaatt
    27121 tcatagaaat tttgatttcg taaacgaaga gacaatctta catgtcactt ctcatttaat
    27181 acgccactac tagacaagca aaatcattat tacagtagtt ccagtccttc aattaacagt
    27241 cacttacaat caaattgagt ttgaactagc tgaagcgacc acagacctat ttcttagtca
    27301 tattcgctaa aaaaatcccc gccaaaatct caaaaagtcc ccgccaattc cccgaccaaa
    27361 atccgaaaaa taccgaaaaa tatcgaaaaa ttatttttag aatagtccca aaaatcctga
    27421 aatagagcta aaaaactcca cctgattcgg tggagttaag ggagattatt atgaaaaaga
    27481 aaagtttagg attttattaa ataaagttag gaggtcttta tttaataact acatgataca
    27541 agacgaaact taaaactagc ttaacttttc taaaatttta ctattttgca aaaaatttct
    27601 atcaccagca cctcac
//
LOCUS       DAVHEX010000025        27085 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVHEX010000025 DAVHEX010000000
VERSION     DAVHEX010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27085
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.025"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..209
                     /locus_tag="VVL45_001046"
     CDS             <1..209
                     /locus_tag="VVL45_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU4187236.1"
                     /translation="RKSSKLKPLTVEDKACNHALSKERSKVENIFAKVKTFKMFSTTY
                     RNHRKRFGLRMNLSAGIINHELGF"
     gene            359..613
                     /gene="blpM"
                     /locus_tag="VVL45_001047"
     CDS             359..613
                     /gene="blpM"
                     /locus_tag="VVL45_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpM"
                     /protein_id="HEU4187237.1"
                     /translation="MDTKIMEQFHEMDITMLSSIEGGKNNWQTNVLEGGGAAFGGWGL
                     GTAICAASGVGAPFMGACGYIGAKFGVDLWAGVTGATGGF"
     gene            629..832
                     /gene="blpN"
                     /locus_tag="VVL45_001048"
     CDS             629..832
                     /gene="blpN"
                     /locus_tag="VVL45_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpN"
                     /protein_id="HEU4187238.1"
                     /translation="MNTYCNINETMLSEVYGGNSGGAAVVAALGCAAGGVKYGRLLGP
                     WGAAIGGIGGAVVCGYLAYTATS"
     gene            1076..1225
                     /gene="blpO"
                     /locus_tag="VVL45_001049"
     CDS             1076..1225
                     /gene="blpO"
                     /locus_tag="VVL45_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HEU4187239.1"
                     /translation="MDTKMMSQFAVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
     gene            1329..1448
                     /locus_tag="VVL45_001050"
     CDS             1329..1448
                     /locus_tag="VVL45_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEU4187240.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     gene            2234..2617
                     /locus_tag="VVL45_001051"
     CDS             2234..2617
                     /locus_tag="VVL45_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HEU4187241.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEAGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKNKLSS"
     gene            2669..3358
                     /locus_tag="VVL45_001052"
     CDS             2669..3358
                     /locus_tag="VVL45_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU4187242.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQLTLIVQNYWQFSSQIGNLF
                     WIQNILSLLFIGVMIVVLVKTGHGYLFRIPRKKWLWYSILTVLVVVFQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYIALFFLVPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFVFATVGIIFAGLTRYTKSIYPSYAVHVINNIVATFP
                     FLLTFLHRVLG"
     gene            3400..3648
                     /locus_tag="VVL45_001053"
     CDS             3400..3648
                     /locus_tag="VVL45_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187243.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLKWFVLERLDLKNDV"
     gene            3678..4289
                     /locus_tag="VVL45_001054"
     CDS             3678..4289
                     /locus_tag="VVL45_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU4187244.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGGFFQNESALILVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMLYNSWPLLYYL"
     gene            4450..5244
                     /locus_tag="VVL45_001055"
     CDS             4450..5244
                     /locus_tag="VVL45_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEU4187245.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            5241..5876
                     /gene="trmB"
                     /locus_tag="VVL45_001056"
     CDS             5241..5876
                     /gene="trmB"
                     /locus_tag="VVL45_001056"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEU4187246.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEAGVSNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            6002..6481
                     /gene="rimP"
                     /locus_tag="VVL45_001057"
     CDS             6002..6481
                     /gene="rimP"
                     /locus_tag="VVL45_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEU4187247.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEMISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            6525..7661
                     /gene="nusA"
                     /locus_tag="VVL45_001058"
     CDS             6525..7661
                     /gene="nusA"
                     /locus_tag="VVL45_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEU4187248.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            7683..7976
                     /locus_tag="VVL45_001059"
     CDS             7683..7976
                     /locus_tag="VVL45_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEU4187249.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            7969..8268
                     /locus_tag="VVL45_001060"
     CDS             7969..8268
                     /locus_tag="VVL45_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEU4187250.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            8285..11161
                     /gene="infB"
                     /locus_tag="VVL45_001061"
     CDS             8285..11161
                     /gene="infB"
                     /locus_tag="VVL45_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEU4187251.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAAPKASAEKKAE
                     KSEPVKPAVAKEEAKPAEPVTPKTEKVAAKPQSRNFKAEREARAKEQAERRKQNKGNN
                     RDQQQNGNRQKNDGRNGGKQGQSNRDNRRFNDQAKKQQGQQKRRNERRQQEDKRSNQA
                     APRIDFKARAAALKAEQNAEYARSSEERFKQYQAAKEALAQANKRKEPEEIFEEAAKL
                     AEQAQQVQAVVEVVPEKKEPAVDTRRKKQARPDKNRDDYDHEEDGPRKQQKNRSSQNQ
                     VRNQKNSNWNNNKKNKKGNNKNNRNQTPKPVTERKFHELPTEFEYTDGMTVAEIAKRI
                     KREPAEIVKKLFMMGVMATQNQSLDGETIELLMVDYGIEAKQKVEVDNADIERFFVED
                     GYLNEDELVERPPVVTIMGHVDHGKTTLLDTLRNSRVATGEAGGITQHIGAYQIVENG
                     KKITFLDTPGHAAFTSMRARGASVTDITILVVAADDGVMPQTIEAINHSKAANVPIIV
                     AINKIDKPGANPERVIGELAEHGVMSTAWGGDSEFVEISAKFNQNIEELLETVLLVAE
                     IQELKADPTVRAIGTVIEARLDKGKGAVATLLVQQGTLNVQDPIVVGNTFGRVRAMTN
                     DLGRRVKVAGPSTPVSITGLNEAPMAGDHFAVYEDEKSARAAGEERAKRALMKQRQAT
                     QRVSLENLFDTLKAGELKSVNVIIKADVQGSVEALSASLQKIDVEGVKVTIVHSAVGA
                     INESDVTLAEASNAFIVGFNVRPTPQARQQAEADDVEIRLHSIIYKVIEEMEEAMKGM
                     LDPEFEEKVIGEAVIRETFKVSKVGTIGGFMVINGKVARDSKVRVIRDGVVIYDGELA
                     SLKHYKDDVKEVTNGREGGLMIDGYNDIKMDDVIEAYVMEEIKR"
     gene            11412..11762
                     /gene="rbfA"
                     /locus_tag="VVL45_001062"
     CDS             11412..11762
                     /gene="rbfA"
                     /locus_tag="VVL45_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEU4187252.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTIIDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
     gene            12041..12820
                     /locus_tag="VVL45_001063"
     CDS             12041..12820
                     /locus_tag="VVL45_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187253.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELDPKDIPTL
                     LEQGPTTLLSQEILDRVANACIRDNVLLASGTSVLAGLPGGLAMAITIPADVAQFYAF
                     SLKLAQELGYIYGYEDLWASREELSEDAQNTLLLYLGVMLGVNGTAALLRVGSITIAK
                     QVMKIVPNKALTKTLWYPILKKVLKIFGVNLTKGGLAKGMGKFIPILGGIISGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIKGE"
     gene            12822..13004
                     /locus_tag="VVL45_001064"
     CDS             12822..13004
                     /locus_tag="VVL45_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187254.1"
                     /translation="MNPIKAFAKIYGNYFLTVQGVKVMKTIKKADHVVVGLGKLFIAD
                     KLMDTARWLIKPEERE"
     gene            complement(13359..13433)
                     /locus_tag="VVL45_001065"
                     /pseudo
     CDS             complement(13359..13433)
                     /locus_tag="VVL45_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            13464..13697
                     /locus_tag="VVL45_001066"
     CDS             13464..13697
                     /locus_tag="VVL45_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEU4187255.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            13697..15031
                     /locus_tag="VVL45_001067"
     CDS             13697..15031
                     /locus_tag="VVL45_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HEU4187256.1"
                     /translation="MTDERIHILRDILLELHNGASPESVQDRFDATFTGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYESMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKEDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKTIMKGLREGSKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            15041..15295
                     /locus_tag="VVL45_001068"
     CDS             15041..15295
                     /locus_tag="VVL45_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEU4187257.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKVGKGFHDLPEGLFGERNY"
     gene            15391..15786
                     /locus_tag="VVL45_001069"
     CDS             15391..15786
                     /locus_tag="VVL45_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEU4187258.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLALKGIGPATIKKLKENGIKFK"
     gene            16199..16648
                     /locus_tag="VVL45_001070"
     CDS             16199..16648
                     /locus_tag="VVL45_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HEU4187259.1"
                     /translation="MIGVVARENAAEQIKQYQKFTVNISDETSMLAMEQAGFISHQEK
                     LERLGVHYEISERTQTPILDACPLVLDCRVDRIVEEDGICHIFAKILERLVAPELLDE
                     KGHFKNQLFAPTYFMGDGYQRVYRYLDKRVDMKGSFIKKARKKDGKN"
     gene            16635..17195
                     /locus_tag="VVL45_001071"
     CDS             16635..17195
                     /locus_tag="VVL45_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4187260.1"
                     /translation="MARTELPDKIETERLVLRVRTVVDAEDIHTYASLPEVAYPAGFP
                     PVKILEDEIYYLEHILPERTQKENLPAGYGIVVKGTDKVIGSVDFNHLHADDVLELGY
                     TLHPDYWGRGYVPEAACSLIDLAFKELGLHKIELTCFGYNLQSQRVSEKLGFTLEARI
                     RDRKDAQGNCCDDLRYGLLRSEWEVI"
     gene            17195..17773
                     /locus_tag="VVL45_001072"
     CDS             17195..17773
                     /locus_tag="VVL45_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU4187261.1"
                     /translation="MHLFIIGAPASGKMTIGQELSRLTDATLFYNHQAIDFALEIYQD
                     YTEEMWEFVRGITFSFLGASARNQRSVILTDVIDFSNQYQLMYLKQIQDLLDNFHQEI
                     LFVELETSLEECIRRNRTENRLKHKPLKRHIEVSEREILETAETLQLNSQHQPNELHH
                     YFKINNTNLSAEEAAKQIQNKINKIEKGHTHV"
     gene            17766..20417
                     /locus_tag="VVL45_001073"
     CDS             17766..20417
                     /locus_tag="VVL45_001073"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEU4187262.1"
                     /translation="MSKELSPKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ITRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VNLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPELGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEQGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDDKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQISEGSIVTAAYPTVNLAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIYLPLADLLNVEEELARLDKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     ECDKQADYQAKYDATVARIDEMKKLVK"
     gene            20498..20662
                     /locus_tag="VVL45_001074"
     CDS             20498..20662
                     /locus_tag="VVL45_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEU4187263.1"
                     /translation="MGKIVAIDLFNGAGGTTSGLKKSGIDVQVAVEIDSVAVKTYKLN
                     NPEVSVIDME"
     gene            20713..22317
                     /locus_tag="VVL45_001075"
     CDS             20713..22317
                     /locus_tag="VVL45_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4187264.1"
                     /translation="MSEYWFSTNVDQIDEVDGKQCLIYSYYNIKASRNVEVLKGRSGT
                     KKGLDYWEPYAPQKQYEMERLPKNKYIGSSSTDRWDGIEKNVVFCDCKEYVSAFDLFF
                     YHYNFKKISTQRSKQDFIRLRSKPVADILKNNTSSYTRYKKEMVIDNVKVDDKVCEII
                     SEIMDESYTDIQILTHKLYSKGDDIKASKTIWMKKSGKEYSEAFAGTGEARIILLVND
                     IVNAQSNSLILIDEPEISLHPSAIYKFKEFLLQECLNKKHQIIITTHSTQLIKDFPRE
                     AVKLLVKNGEKVDVIENIDYQDAFFELGDVYHSRKMIYVEDRLAKYILEFVITHSGSE
                     NLKQNLVVRYIPGGANQIICNNILNSSYLDSDNHYFWLDGDQNTNVSESNNLMNYLEN
                     GVVISDKIPESDNKNLDDIIKLITGCPIKFNVSGNKGQKNNIELIAKQRSFIDYWAKY
                     VSYLPFPTPEFFLANLCNSVDREGYDFSKDGNGKEYFRKKTQVALGIENITSEDIFQE
                     QRRAVSKIQPESSMFQCIKEKLEALF"
     gene            22697..23500
                     /locus_tag="VVL45_001076"
     CDS             22697..23500
                     /locus_tag="VVL45_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HEU4187265.1"
                     /translation="MQPTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPAILLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADMVKKIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNTPFQQHSKY
                     FRDALVLDNAKILQRRPEFLTAFFENLLLGGQNDLSSEKMYLDLDLDLS"
     gene            complement(23615..24815)
                     /locus_tag="VVL45_001077"
                     /pseudo
     CDS             complement(23615..24815)
                     /locus_tag="VVL45_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            25363..26228
                     /locus_tag="VVL45_001078"
                     /pseudo
     CDS             25363..26228
                     /locus_tag="VVL45_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442363.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            26331..27085
                     /locus_tag="VVL45_001079"
                     /pseudo
     CDS             26331..27085
                     /locus_tag="VVL45_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268095.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
BASE COUNT         8306 a         4750 c         5987 g         8042 t
ORIGIN      
        1 cacgtaaatc cagcaaactc aaaccgctaa cagttgaaga taaagcctgt aaccatgcgc
       61 tatctaagga gagaagcaag gttgagaaca tctttgccaa agtaaaaacg tttaaaatgt
      121 tttcaacaac ctatcgaaat catcgtaaac gcttcggatt acgaatgaat ttgagtgctg
      181 gtattatcaa tcatgaacta ggattctagt tttgcaggaa atctaatgta atttttgcaa
      241 ttcaagacgt ttcgatgact attcaagatc tggatgaaaa attttaaaaa acgatgatat
      301 actaaacttg tcaaagttac aacaagacaa aaattaaaaa taaaaaagag tatttgtcat
      361 ggatacaaaa attatggaac aatttcatga aatggatata actatgttat ctagtattga
      421 aggaggcaag aataattggc aaactaatgt cttagaaggt ggtggtgctg cttttggtgg
      481 ctgggggcta gggacagcta tttgtgctgc gagtggtgtt ggagcaccat ttatgggagc
      541 atgtggatac ataggagcta aatttggtgt ggatctttgg gcaggagtaa caggggcaac
      601 gggtggattt taataaaagg agacttgtat gaatacttat tgtaatataa atgaaacaat
      661 gctgagtgag gtttatggag gtaattccgg aggagcagct gtagttgctg ctttaggttg
      721 tgcagcgggt ggagtgaaat atgggagact tctaggacca tggggcgctg caataggagg
      781 aattggagga gcagtagttt gtggatattt agcctatacc gctacatcat aaaaatgaaa
      841 gctaaatttt atgaaggcta tattctttat tattctcttc gcttttcaaa cctatcttat
      901 ttatctatcc ataagtatct ccgataaaaa acaaaagact attgaattaa catcattgaa
      961 ttgttttgtc atattatttc taatctatga taaattaatt tttctcttta ttgcctatgt
     1021 ttttttgata atttttatat taaacttgtt ccgtaactga aaagtgatat actatatgga
     1081 tacaaaaatg atgtcacaat ttgcagttat ggataatgaa atgcttgctt gcgttgaagg
     1141 tggagatatt gattggggaa gaaaaattag ttgtgcagca ggggttgcat atggcgcaat
     1201 tgatgggtgt gcaacaacgg tttgacattt ctattgggac catttgctat aggaataggt
     1261 gtaactggtg ctgcaggtgg agctatactt ggaggtgtag cctatgcagc aatatgttgg
     1321 tggtaattat ggattttaaa agttttatta ttggtttagt agttggtata tttggtcctt
     1381 atatggatga cttaattaga aaaatatttt caaaatcttc caaaaaggat acggatagta
     1441 ctctctaaaa aataaatttc tcccctgtat ttgctagctt aatttacagg ggagtttctt
     1501 taatattgtt tcgcctgtca aaatgatatg tgatagcagg atgaaaagtc tacaaacaag
     1561 ttggtatcaa tacaatataa agaggaaagt atgtttttaa tgcttacttt ttaaattttt
     1621 taacaattca agatgtttcg atgacaattc aagatttgga tgaaaaattt taaaaaacaa
     1681 tgatatacta aacttgtcaa agttgcaacg ggtgctgcta cattatgtta aaaatcgctc
     1741 ttgattgata agttggattt gttaggaaag gagtaaataa ttatggataa gaaaaagata
     1801 gtttcgacta taatatgtat agtatttctt gtagtgtcag tagataattt ttttagagac
     1861 ttaaccccgt tactgttcat tttaaatatt attggcctgt cttgcttttc agtgctaaca
     1921 tacattaaca taaaagagat actcttaaat ataagtaaat gatgatttgg ttagtcctag
     1981 aaccaatcaa cttatgttta tcggcttggc agattttatg tttgtcattt gtttgtatag
     2041 aggtatttca gaaacagaat tctatcaaca actaatcgct tgtataggtg tattctctgc
     2101 ttgtttatca cgcttctgct catgtggggc ttgaagtatt ataaaaagta gctttctatc
     2161 cttttctgac aggatatttg agtagaaggc tctattaaat tgttagtgaa ataaatgaat
     2221 ggaggtattt aatatgaaat acagattatt ttttgttatt ttcttgagta gtgtgttgga
     2281 tattctttta gggacatttt tacaaatctc tatcgtatct attgggtggc ttgttcttta
     2341 cagtggtttg tttgaagcag gagttttcct tcttgctaat aaaggggtgg cggtaaaaat
     2401 caaggaagta gatattcgaa atcgctttaa atttattttt ggaaaaacct tatggtttca
     2461 aattcttttg ctcatctttt tgataatcaa actttatctt ggtttggatg cgaggttgat
     2521 tttattctat ggacatattt tcattgtctt taatgcctta atgtatctat tatctagtag
     2581 tcaggttagc cttaaaaaaa acaaactgtc ttcttaatct tacagcagta gagcctgaca
     2641 aggtgaggtc atagtaggga ggatttctat gaaaaagtat caacttctat tcaaaataag
     2701 tgcagtcttc tcttacttat ttttcgtatt tggtctttct cagctgacgc ttatcgtcca
     2761 aaactattgg caattttctt ctcagatagg caatttattc tggattcaaa atatcttgag
     2821 tttacttttt attggagtca tgattgtggt tcttgttaag acaggccatg gttatctctt
     2881 ccgcattcca agaaaaaaat ggctttggta ttcgattttg acagtattag tggtagtgtt
     2941 ccagatctct tttaacgttc agacagctaa acatgttcag tcaactgcgg aaggttgggc
     3001 tgtattgatt ggttatagtg ggactaactt tgcagagcta ggtatttata tagccctgtt
     3061 ctttctggtt ccactgatgg aagaattgat ttatagagga ttactgcaac atgctttctt
     3121 taagcattcg cgatttggtc ttgatttgct tcttccttct attttatttg ctctccctca
     3181 tttttcaagc ctgcctagtc tgttagatat cttcgtcttt gcaacagttg gaatcatctt
     3241 tgctggtttg acccgctata ccaagagcat ttatccatcc tatgcggtgc atgtgatcaa
     3301 taatattgta gcgaccttcc cgtttttgct cacttttcta catagggtct tggggtaaaa
     3361 aaacaaaagg cttgcttttc agccatagag gaggtcatca tgtataaaca cttatttttc
     3421 ctagattcca aaactttaga tcggttgaca ccctatattc tagtcttggc ttctgacacc
     3481 attgctttta atgtttttgt gctaaccttt gtatctgcgg tggtttttaa tttcctaaat
     3541 tccatgctag ctttaatggc tatattcata ggggctggct atgtggtcgg attttggttg
     3601 ctaaaatggt ttgttttgga aaggctagat ctaaagaatg acgtgtagga agtctgtttg
     3661 ttgaggagga taattttatg gagttttttg ataaatttca tgccttgtgt tttggatttt
     3721 tagtactaat aattgtcatt acagttcctt atacgattaa ccatgggggt ttttttcaaa
     3781 atgaatctgc attgattctt gtaagtcttc ttgtaacctc gctgagtgtt gcttatgcta
     3841 gaaagtttga aatgatttct tttgggatgt taagcaagaa acaacttttg cttttcattg
     3901 caatctttct tctaagtgta cttgagacgc tggtttatat tcatttcttc gctgtttctt
     3961 ctggctcagg ggtccaacac ttggcggaag tcagcagagg aatttccctg tctttgattt
     4021 taactacctc agtttttggc cccatccagg aggaactcat tttcagagga cttcttcaag
     4081 gtgcggtttt tgacaattct tggttagggc ttgtgctaac ttcctctctc ttttctttca
     4141 tgcatggacc ttctaatgtc ccttcgttta ttttttatct acttgggggt ttgttgctgg
     4201 gctttgctta taaaaagagt caaaacctat gggtttctac tctagtccat atgctttaca
     4261 acagttggcc actcttatat tatttataaa aattatgaaa aggtaagcag aagacggtgc
     4321 ttaccttttt caagctataa tgatacccaa gccaatccag aggcttttct ttgagaatcg
     4381 ggttagagtg gttgacgaat aggccaaaaa ctagtagaat agtaaggaaa ctttatacgg
     4441 aggaaagaaa tggatttggg tgataatgag ctaacactga ctcccatacc tgggaaaagt
     4501 ggcaaggctt atatgggtag ctatcctgat gggaagcgca tctttgtaaa aatgaacacc
     4561 tctccaatcc tacctggtct agctagagaa caaattgctc cacaattatt atggagtcgt
     4621 cgtttggcag atgggcgtga tatgtgtgct caagaatggt tgacaggcaa gatattgacc
     4681 ccctatgata tgaatcgtaa gcaaatcgtc aatattttaa cccgtcttca tcgctcacgt
     4741 ccgttgatga cacaattgag tcgtttgggc tatgccatgg aaacacctgt agatttacta
     4801 cagtcttggc aggaaacggc tccagatgct ttgcgtaaaa atcattttat cagtgaagtg
     4861 atggctgatt tacgtcagac tattccagga tttagagagg accatgcgac cattgtccat
     4921 ggagatgtac gacatagtaa ttggattgag acagatagtg gcttgattta tttagtagat
     4981 tgggattcgg ttcgcttgac cgatcgcatg tttgatgtgg cccatatgct ctgccattat
     5041 atttcagaac atcagtggaa ggaatggttg acctactacg gttacaagta caatcaaacg
     5101 gtattaagta aattgtattg gtatggtcaa ttgtcttatt tgagtcagat ttccaagtat
     5161 tatatgaacc aagatttaga aaatgtcaat cgggagattc atggtttgcg tcatttccga
     5221 gacaagtatg gaaagagaag atgagagtta gaaatcgtaa aggggcaaca gaattactag
     5281 aggcaaatcc ccagtatgtg gtcctcaatc ccttggaagc caaggcaaaa tggcgggact
     5341 tgtttggcaa tgataatccc attcatgtgg aagttggaag tggaaagggt gcctttgttt
     5401 caggtatggc caagcaaaac cctgacatca actatatcgg gattgatatt caaaagtctg
     5461 ttttgagcta cgctttggac aaggtgcttg aagctggagt gtctaacatc aagctcttgt
     5521 gggtagatgg ttctgacttg actgactact ttgaagacgg tgagattgat cgcttgtatc
     5581 tgaacttttc agatccatgg ccgaaaaaac gccatgaaaa gcgtcgtttg acctacaaga
     5641 cctttttgga taccttcaaa cgtatcttgc ctgaaaatgg agaaattcat ttcaagacgg
     5701 ataaccgtgg cttgtttgag tacagtttag tgagcttttc tcaatatggc atgaaactca
     5761 atggtgtctg gctagatttg catgccagtg attttgaagg caatgtcatg acagaatacg
     5821 agcaaaaatt ctcaaacaag gggcaagtta tctaccgagt tgaggcagaa ttttaagaga
     5881 tagcctaaaa ttaggctgta caagtgcttt tgctttacat aagttggcaa acgtgctata
     5941 ctgatagtaa gaatatgaaa agtgaggcgg ggaaatatct tcgcctcttg cttatgagga
     6001 ggtggacgca atcgcaacaa tcgtagaatt agtcagagaa gttgtagaac ctgtcataga
     6061 agctcctttt gaactcgtgg atatcgagta tggaaagatt ggcagtgaca tgattctcag
     6121 tatttttgta gataaacccg aaggaattac cttgaacgac acggcagact tgacagaaat
     6181 gatcagtcct gtcctagaca ccatcaagcc agatcccttc ccagaacaat atttcctaga
     6241 aattaccagt ccaggtttgg aacgtccttt gaaaaccaag gatgccgtcg ctggagcggt
     6301 tggaaaatac atccatgtcg ggctctacca agccatcgat aagcaaaagg tctttgaagg
     6361 aaccttgttg gccttcgaag aggacgagtt gactatggaa tatatggaca agacgcgtaa
     6421 gaaaaccgtc caaattccat acagtttagt atcaaaagca cgtttagcag ttaaattata
     6481 gaaaaagaaa ggatagcttt tgaggattca aaagtgaaga aaacatgagt aaagaaatgc
     6541 tagaggcctt ccgcattttg gaagaagaca agggaatcaa aaaagaagat atcatcgacg
     6601 cagtagtaga gtcgcttcgt tccgcttatc gcagacgcta tggtcagtca gacagcgtag
     6661 ctattgactt caacgaaaaa acaggtgact ttacagttta tactgtccgt gaagttgttg
     6721 atgaagtatt tgatagccgt ttggaaatca gcttgaaaga tgctcttgcc attaattcag
     6781 cttatgaact tggagacaaa atcaagtttg aagaagcacc agctgagttt ggtcgtgtag
     6841 cagcccaatc tgccaaacaa accatcatgg aaaaaatgcg caagcaaaca cgtgccatca
     6901 cttacaatac ttacaaagaa catgagcaag aaatcatgtc tggtacagta gaacgctttg
     6961 acaaccgctt tatctatgtc aaccttggta gcatcgaagc ccaattgtca aaacaagacc
     7021 aaattcctgg agaagttttt gcttctcatg atcgtatcga agtttatgtt tacaaggttg
     7081 aagacaaccc tcgtggtgtg aacgtctttg ttagccgtag ccatccagaa atgatcaaac
     7141 gtttgatgga gcaagaaatt ccagaagttt atgatggaac tgttgaaatc atgagcgtgg
     7201 ctcgtgaagc aggtgaccgt acgaaggttg ctgttcgtag tcacaatcca aacgtggatg
     7261 ctatcggtac aatcgttgga cgtggtggtg ctaatatcaa gaagattact agcaaattcc
     7321 acccagctcg ttacgatgct aaaaatgacc gcatggtacc aatcgaagaa aatatcgatg
     7381 ttatcgagtg ggtagcagat ccagctgaat ttatctacaa tgccatcgct cctgctgagg
     7441 ttgaccaagt tatctttgat gaaaacgaca gcaaacgtgc cttggtggtt gttccagata
     7501 acaagctttc tcttgccatt ggtcgtcgtg gacaaaacgt gcgcttggcg gctcacttga
     7561 ctggttaccg tatcgatatc aagtctgcta gcgaatttga agccatggaa gacgctgctt
     7621 cagtagagtt ggaagtagaa aacgatactg tagaagaata aaagctgcta gaggagggaa
     7681 agatgaaaac aagaaaaatc cctttgcgca agtctgttgt gtctaacgaa gtgattgata
     7741 agcgtgattt gctccgcatt gtcaagaaca aggaaggaca agtctttatt gatcctacgg
     7801 gcaaggccaa tggccgcggc gcttatatca aactagacaa tgcagaagcc ctagaggcga
     7861 aaaagaagaa ggtctttaac cgcagcttta gcatggaagt ggaagaaagc ttttatgacg
     7921 agttgatcgc ttatgtggat cacaaagtga aaagaagaga gttgggactt gaataagcaa
     7981 aagataagta atctcttggg gcttgctcag cgagcagggc gcatcatatc gggtgaagaa
     8041 ttggtggtca aggccattca agacggcaag gccaagttgg tctttctagc tcatgatgct
     8101 ggacccaatc tgaccaagaa gattcaagat aaaagtcatt attatcaagt agaaattgta
     8161 accgtgtttt caacactgga attaagcata gcagtcggga aatcgagaaa ggttttggct
     8221 gtaacagatg ctggatttac aaagaaaatg aggtctctta tggaatagaa gaggaggaca
     8281 tgatttgtct aagaaaagat tgtacgaaat cgcaaaagaa cttggaaaag aaagtaaaga
     8341 agttgtagcg cgtgcaaaag agttgggctt ggatgtgaaa agccactcat caagtgtgga
     8401 agaagctgtc gctgcaaaaa ttgctgccag ctttaagcct gcagctgctc cgaaagtaga
     8461 agcaaaacct gcagccccaa aagtaagtgc agaaaagaaa gccgaaaaat ctgagccagc
     8521 taaaccagct gtagctaagg aagaggcaaa acctgcagcc ccaaaagcaa gtgcagaaaa
     8581 gaaagccgaa aagtctgaac cagtaaaacc agctgtagcc aaggaagagg caaaaccagc
     8641 tgagccagtc actccgaaaa cagaaaaagt agcggctaaa ccgcaaagtc gtaatttcaa
     8701 ggctgagcgt gaagcacgtg ctaaagagca ggcagagcga cgcaagcaaa ataagggcaa
     8761 taaccgtgac caacaacaaa acggaaaccg tcagaaaaac gacggccgta atggtggaaa
     8821 acaaggtcaa agcaaccgcg acaatcgtcg ctttaatgac caagctaaga agcagcaagg
     8881 tcagcaaaaa cgtagaaatg agcgccgtca gcaagaggat aaacgttcaa atcaagcggc
     8941 tccacgtatt gactttaaag cccgtgcagc agccctaaaa gcagagcaaa atgcagagta
     9001 cgctcgttca agtgaggaac gcttcaagca gtatcaggct gctaaagaag ccttggctca
     9061 agctaacaaa cgcaaggaac cagaggaaat ctttgaagaa gcggctaagt tagctgaaca
     9121 agcacagcaa gttcaagcag tggttgaagt cgtccctgag aaaaaagaac ctgcagtgga
     9181 tacacgtcgt aaaaaacaag ctcgaccaga caaaaatcgt gacgattatg atcatgaaga
     9241 agatggtcct agaaaacaac aaaagaatcg aagtagtcaa aatcaagtga gaaatcaaaa
     9301 gaatagtaac tggaataaca acaaaaagaa caaaaaaggc aataacaaga acaaccgtaa
     9361 tcagactcca aaacctgtta cggagcgtaa attccatgaa ttgccaacag aatttgaata
     9421 tacagatggt atgaccgttg cggaaatcgc aaaacgtatc aaacgtgaac cagctgaaat
     9481 tgttaagaaa cttttcatga tgggtgtcat ggccacacaa aaccaatcct tggatgggga
     9541 aacaattgaa ctcctcatgg tggattacgg tatcgaagcc aaacaaaagg ttgaagtgga
     9601 taatgctgac atcgaacgtt tctttgtcga agatggttat ctcaatgaag atgaattggt
     9661 tgagcgtcca ccagttgtta ctatcatggg acacgttgac cacggtaaaa caaccctttt
     9721 ggatactctt cgtaactcac gtgttgcgac aggtgaagca ggtggtatta ctcagcatat
     9781 cggtgcctac caaatcgtgg aaaatggtaa gaagattacc ttccttgata caccaggaca
     9841 cgcggccttt acatcaatgc gtgcgcgtgg tgcttctgtt accgatatta cgatcttggt
     9901 cgtagcggca gatgacgggg ttatgcctca gactattgaa gccatcaacc actcaaaagc
     9961 agctaacgtt ccaatcatcg tagctattaa caagattgat aaaccaggtg ctaacccaga
    10021 acgcgttatc ggtgaattgg cagagcatgg tgtgatgtca actgcttggg gtggagattc
    10081 tgaatttgtt gaaatttcgg ctaaattcaa ccaaaatatc gaagaattgt tggaaacagt
    10141 ccttcttgtg gctgaaatcc aagaactcaa agcagaccca acagttcgtg cgatcggtac
    10201 ggttatcgaa gcgcgcttgg ataaaggaaa aggtgcggtc gcaacccttc ttgtacaaca
    10261 aggtaccttg aatgttcaag acccaatcgt tgtcggaaat accttcggtc gtgtccgtgc
    10321 tatgaccaac gaccttggtc gtcgtgttaa agttgctgga ccatcaacac cagtctctat
    10381 cacaggtttg aacgaagcac cgatggcggg tgaccacttt gccgtttacg aggatgaaaa
    10441 atctgcgcgt gcagcaggtg aagagcgtgc caaacgtgcc ctcatgaaac aacgtcaagc
    10501 tacccaacgt gttagccttg aaaacctctt tgataccctt aaagctgggg aactcaaatc
    10561 tgttaatgtt atcatcaagg ctgatgtaca aggttctgtt gaagcccttt ctgcctcact
    10621 tcaaaagatt gacgtggaag gtgtcaaagt gactatcgtc cactcagcgg tcggtgctat
    10681 caacgaatca gacgtgaccc ttgccgaagc ttcaaatgcc tttatcgttg gtttcaacgt
    10741 acgccctaca ccacaagctc gtcaacaagc agaagctgac gatgtggaaa tccgtcttca
    10801 cagcattatc tacaaggtta tcgaagagat ggaagaagct atgaaaggga tgcttgatcc
    10861 agaatttgaa gaaaaagtta ttggtgaagc ggttatccgt gaaaccttca aggtgtctaa
    10921 agtgggaact atcggtggat ttatggttat caacggtaag gttgcccgtg actctaaagt
    10981 ccgtgttatc cgtgatggtg tcgttatcta tgatggtgaa ctcgcaagct tgaaacacta
    11041 taaagacgac gtgaaagaag tgacaaacgg tcgtgaaggt ggattgatga tcgacggcta
    11101 caatgatatt aagatggatg atgtgattga ggcgtatgtc atggaagaaa tcaagagata
    11161 agattttttg ctcctttctt aggtggtgag ggacgcaagc aaaccgatgg tttcattgct
    11221 tatttttgag cctagggtct caaaaatccc ctgtgatggg actgataaat cagttccatc
    11281 actttcacca cggcgaaaga agcagatgac ttcaaattga acttcgtttc aatttaaact
    11341 gaaaatcaag aagtttaaaa tagctaggtc tgctggccta gcttttggtt caaagtagag
    11401 aaaggaatat catggcaaat catttccgta cagatcgtgt gggcatggaa atcaagcgtg
    11461 aagtcaatga gattttgcaa aagaaagtcc gtgatccacg tgtccaaggt gtgaccatca
    11521 tagatgttca gatgctgggt gacttgtctg ttgccaaggt ttattacacc attttgagta
    11581 accttgcttc ggataaccaa aaagcccaaa tcgggcttga aaaagcaact ggtaccatca
    11641 aacgtgaact tggtcgcaat ttgaaattgt acaaaatccc agatttgacc ttcgtcaaag
    11701 acgagtccat cgagtatgga aacaagattg acgagatgct acgcaatctg gataagaact
    11761 aaagaagagg ggttgcccct cttttttggt ggaggaaaat aggttgaatt tgaaatggaa
    11821 aaatattctt ttataataga ttgaaactag aatagtacgc ctctacttct aaaatattgt
    11881 tagaaatcga tttgactgtc ctgatcgatt tgtcctgttc ttgtttcatt ttaatataaa
    11941 aaagggattc tgtatttttt aatgttatct aattagaaaa tgcttttttt gtaggaaata
    12001 taatatgata aggtgcaaaa aagaaataag gagtttgtat atggctgaac aagacttagc
    12061 tatgcaagta ttgcaacaag tggtgaaact acctgttgtt aaggttgatc gttcgaaatt
    12121 tttagtggat aagttttcca aagaattgga tccaaaagat attcctacct tattggaaca
    12181 aggtccaacg actcttctat ctcaagaaat attagatcgt gtagctaatg cttgtattcg
    12241 ggacaatgta ttattagcga gtgggacttc tgttttggca ggattacctg gagggcttgc
    12301 tatggcaatt accattccag ctgatgtggc tcaattttat gctttctctc tgaaattggc
    12361 tcaagaatta ggttatattt atggttatga ggatctttgg gcttcacgag aggagttgag
    12421 tgaagatgct caaaataccc tcttgcttta tctaggcgta atgttagggg tgaatggaac
    12481 cgctgctttg ctacgtgttg gtagtataac aattgccaaa caggtaatga aaatagtgcc
    12541 taataaagct ttaacaaaga cgctttggta ccctattttg aaaaaagtct taaaaatatt
    12601 tggtgtgaat cttaccaagg gagggttggc caaaggaatg gggaaattta ttcctatctt
    12661 gggtggtatc atttcaggtg gtttaacctt tgcaactatg aaaccaatgg gggaaagctt
    12721 gcagaaagaa ttatccaagc tagtcaacta tagtgaagtt caatatcaag aagatgttga
    12781 aacaatccga aaagaggctg aaatcatcaa aggagagtaa tatgaatcct atcaaagctt
    12841 ttgctaaaat ttatggtaat tactttttga ccgtgcaagg tgtaaaagtg atgaaaacga
    12901 taaagaaagc tgaccatgtc gttgttggtc tggggaaact ttttattgcc gacaagttaa
    12961 tggatacggc tcggtggctc attaagccag aggagagaga atgaaatttt tttggtcttc
    13021 ttgctattct ttttatcaaa ccgattattg ggattgtgaa attcttttgg atgatcatct
    13081 cttttgcagt ccaattgctg ttttacaaga tagtgtttaa gatattggat tggctcttta
    13141 aacttatcta gatggtaatc caagttgcag agaactagca ggaactccac tgctagtttt
    13201 ttattctctt tccatatggt ataatataag cagtaaaatc attttatact cttcgaaaat
    13261 ctcttcaaac cacgtcagct tcaccttgca gtacatatgt tactgacttc gtcagttcta
    13321 tccacaacct caaaacggtg ttttgagctg acttcgtcag ttctatctac aacctcaaaa
    13381 cactgttttg agcaacctgc ggctagcttc ctagtttgct ctttgatttt cattgagtat
    13441 tagaacatac aatggaggtc gtcatggaca atatcatcga tgtgtcaatt cctgttgcag
    13501 aagtggtgga caagcatcca gaagtcttgg aaattctagt ggagttgggt tttaaacccc
    13561 ttgccaatcc cttaatgcgc aatacagttg gtcgtaaagt atcacttaaa cagggttcta
    13621 agctagcagg aactcctatg gacaagattg tacgcacact ggaagcgaat ggctacgaag
    13681 tgattggatt agactaatga cagatgaacg gattcatatc ctacgggata ttttgttaga
    13741 attgcacaat ggcgcctctc ctgagtcggt tcaagatcgc tttgatgcga cctttacggg
    13801 cgtgtcagcc atcgagattt cccttatgga gcacgagctg atgaactcgg attcgggcgt
    13861 cacttttgaa gatgttatgg aactctgtga tgtccatgcc aatcttttta aaaatgctat
    13921 caaaggtgtc gaagtttcag atactgagca tccaggtcac ccagttcgtg tcttcaaaga
    13981 agaaaatctg gctctccgtg cggccttgat tcgcattcgt agattgttag atacctatga
    14041 gtctatggaa gacgaggaaa tgctggcgga gatgcgtaag ggtttggtgc gtcagatggg
    14101 acttgtgggt caatttgaca tccattacca acgtaaggaa gaactcttct ttcctatcat
    14161 ggagcgctat ggacacgatt cacctcccaa agttatgtgg ggagtggatg atcagattag
    14221 ggaactcttt caaacagctc taacgacagc caagtcacta ccagaagtgt caattagcag
    14281 tgtaaaggaa gcttttgaag cttttgcgac agagtttgaa agtatgattt tcaaggaaga
    14341 gtccatcctc ctcatgattc tccttgagtc ttttactcag gatgactggc ttcagattgc
    14401 ggaggagagc gatgcctatg gctatgccat catccgtccg tcagagaaat gggtgccaga
    14461 acgacagagc tttattgagg aaaagattgc agaggagcct gtacagctag atacggcaga
    14521 aggtcaagtt caacaagtca tagatacgcc agaaggccat tttaccatta cctttacccc
    14581 taaggaaaag gaagctgtgc tggaccgcca tagtcaacag gcttttggta atggctatct
    14641 ttcagtcgag caggccaatc tcatcctcaa tcatctccct atggagatta cctttgtcaa
    14701 taaagaagat attttccagt attacaatga caatacgcca gctgatgaga tgattttcaa
    14761 acggacgccg tcccaagtcg ggcgcaatgt cgaactctgc catccgccta agtacttgga
    14821 caaggtcaaa actatcatga aggggcttcg tgagggaagc aaagacaagt atgaaatgtg
    14881 gttcaagtct gagtcgcgag gtaagtttgt ccacatcacc tatgctgcag tacacgatga
    14941 agacggagaa ttccaaggag tgttggagta tgttcaggat atccagccct accgtgagat
    15001 tgatacggac tattttcgtg gattagaata aggagaaaaa atgagttacg aacaagaatt
    15061 tatgaaggaa tttgaagctt gggtcaatac ccaaatcatg attaacgaca tggcgcacaa
    15121 ggaaagccaa aaagtttacg aagaagacca ggacgagcgt gccaaagatg ccatgattcg
    15181 ctacgagagt cgcttggatg cttatcagtt cttgcttggt aagtttgaaa acttcaaagt
    15241 aggcaaggga ttccatgatt tgccagaagg cttgtttggt gagcgaaatt attaaacgag
    15301 aaagattctt gatttttcac taaaatcttg atagaatgtt tatgttaaat ccttgtcaga
    15361 gcagggattt tttattgaaa ggattttatc atgtcaaaga aactcaatcg taaaaaacaa
    15421 ttacgaaatg gcctccgtcg cgcaggtgcc ttttcaagta cggtgactaa ggttgtagat
    15481 gagacaaaaa aagtcgtgaa gcgtgcagaa cagtcagcaa gcgcagctgg taaggctgtt
    15541 tctaaaaaag ttgaacaagc agtagaagct accaaagagc aagctcaaaa agtagctaat
    15601 tctgtagaag attttgcagc aaatttgggt ggacttccac ttgatcgtgc caagactttc
    15661 tatgatgaag gaatcaagtc tgcttcagat ttcaaaaact ggactgaaaa agaactcctt
    15721 gccttgaaag gaatcggccc agctaccatc aagaaattga aagaaaatgg catcaagttc
    15781 aagtaatttt tcttgagcct tgcatttccg aaaaaatctt gctacaatag agccattaga
    15841 ggtgttttga atcccacatt ttacagaaag tggcggcgct gagaagtcca caaatgtgtc
    15901 aaaactggtt gctaatggat gaaaaattga aataaaagtg tctttttgct ttaaagacga
    15961 gagttgcggg caactgcccg aaattgggtg gtaccgcgga taaacacatt cgtccctgtc
    16021 atgtagatgg cagggctttt cttttacgtc ttagtcaaag ggggttgtta tgaagcaatc
    16081 ttttaacaca agtaaactat attatggttt tcctgttttt attttaggat atcaagatta
    16141 gaattttggc tataacatca cgacctgtag ttcctcttat agtctaggag attgggttgt
    16201 gattggagtc gttgcgagag agaatgccgc agagcagatc aaacagtatc aaaaatttac
    16261 tgtgaatatt tctgatgaaa cttctatgct tgcgatggag caggctggtt ttatcagtca
    16321 tcaggagaaa ttggaacgtt tgggagtgca ttatgaaatt tctgaacgaa ctcagactcc
    16381 tattttagac gcctgtccac ttgttttaga ttgtcgggta gataggattg ttgaggaaga
    16441 cggtatttgc cacatctttg ccaagattct tgagcgactt gttgccccag aactcctgga
    16501 tgaaaaggga cattttaaaa atcaactgtt tgccccaacc tatttcatgg gagatggata
    16561 tcagcgcgtt tatcgctatc tggacaagcg tgtagatatg aagggcagtt tcatcaaaaa
    16621 agcgaggaag aaggatggca agaactgagc tgccagataa aatcgaaaca gaacgtctcg
    16681 ttttacgagt ccgcacagtg gtggatgctg aggatatcca tacctacgct agtttgccag
    16741 aggtcgccta tccagcaggt tttcctccag tcaagatctt ggaagatgag atttattatc
    16801 tggagcacat tcttccggag cgtactcaaa aggaaaatct cccagctggc tatgggattg
    16861 tcgtcaaagg aaccgataaa gtcattggtt ctgttgactt caaccatctt catgcggatg
    16921 atgtgctaga gcttggctat accttgcacc cagactattg gggtcgaggt tatgttccag
    16981 aagcagcgtg ttccttgatt gatctagctt ttaaagaact tggtcttcac aagattgaac
    17041 taacttgctt tggatataac cttcaaagtc aacgagtctc ggaaaagctt ggctttaccc
    17101 tcgaagctcg cataagagac cgcaaagatg ctcaaggcaa ctgctgtgat gatttgagat
    17161 atggcttgct gaggagtgag tgggaggtga tttgatgcat cttttcatca ttggtgctcc
    17221 agcatcagga aaaatgacca ttggtcaaga attatctcga ctgacggatg ctaccctatt
    17281 ttataaccat caagccattg attttgcact agagatctat caggattata ccgaggagat
    17341 gtgggaattt gttcgtggaa ttaccttttc tttccttgga gcaagtgcaa gaaatcagcg
    17401 atcagtaatt ttaacagacg taattgattt ttcaaatcag taccagctga tgtatttaaa
    17461 gcaaattcag gatttattgg ataactttca tcaagagatt ctgtttgttg aattggaaac
    17521 aagtcttgaa gagtgcatac gtcgaaatcg aacggagaat cgattaaagc acaaaccctt
    17581 aaaacgacat attgaggtat ctgaaagaga aattttagag accgctgaaa cacttcaatt
    17641 aaattcccaa catcaaccga atgagttgca ccactacttt aaaataaata atacgaattt
    17701 gtctgcagaa gaagctgcta agcaaattca aaataaaata aacaaaatag agaaaggaca
    17761 cacacatgtc taaagaactt tcacctaaat acaatccagc cgaggttgag gctggtcgtt
    17821 accaaaaatg gcttgatgcg gatgttttca agccttcagg cgatcaaaag gctaagcctt
    17881 attcaatcgt gattccacca ccaaacgtaa ctgggaaact tcaccttggt cacgcttggg
    17941 atacgacttt gcaggatatc atcatccgtc aaaaacgcat gcaaggcttt gatacccttt
    18001 ggcttccagg tatggaccac gctggtattg cgactcaggc taaggttgag gagcgcttac
    18061 gtggtgaggg tattacgcgt tatgacctag gtcgtgagtc tttcttgacg aaggtctggg
    18121 aatggaaaga cgaatatgcc actaccatca aggaacaatg gggcaagatg gggctttctg
    18181 tagactattc tcgtgagcgt ttcactcttg atgaaggttt gtcaaaagct gttcgtaagg
    18241 tctttgtgaa cctttacaag aaaggctgga tctatcgtgg tgagtttatc atcaactggg
    18301 acccagcagc tcgcacagcc ctttctgata ttgaggtgat tcacaaggat gtagaaggtg
    18361 ccttctacca catgaattac atgctggaag atggttcacg cgcccttgaa gttgctacaa
    18421 ctcgtcctga gactatgttt ggggacgttg cggttgcggt taatccagaa gacccgcgct
    18481 acaaggactt gattggtaaa aacgtcatcc ttccaatcgc taataaactc atcccaatcg
    18541 ttggagatga gcacgcagat cctgagcttg gtactggtgt cgtgaaaatc acacctgccc
    18601 acgatccaaa tgacttcttg gttggccaac gtcataactt gccacaagtc aacgtcatga
    18661 acgacgacgg aactatgaat gagcttgcct ttgaattttc aggcatggat cgttttgaag
    18721 ctcgtaaggc agtcgttgct aagttggaag aaatcggtgc ccttgtcaaa atcgaaaaac
    18781 gtgtccacag tgttggtcac tcagaacgta caggtgtcgt agttgagcca cgcttgtcta
    18841 ctcaatggtt cgtcaagatg gaccaattgg ctaagaacgc cattgctaac caagacacag
    18901 aggacaaggt cgaattctac ccacctcgtt tcaacgatac cttccttcaa tggatggaaa
    18961 atgtccacga ctgggttatc tctcgtcagc tttggtgggg tcaccaaatc cctgcctggt
    19021 acaatgctga tggtgaaatg tatgtcggcg aagaagctcc agaaggtgac ggatggactc
    19081 aggacgaaga cgtcttggat acttggttca gttctgccct ctggccattt tcaaccatgg
    19141 gctggcctga agtcgactca gaagacttta aacgttattt cccaacttca accttggtaa
    19201 caggttacga catcatcttc ttctgggtgt ctcgtatgat cttccagtcc ttggaattta
    19261 ctggtcgtca gccattccaa aacgtgctta tccacggtct catccgtgac gagcaaggac
    19321 gcaagatgtc taaatctctc ggtaacggga ttgacccaat ggatgtcatc gagaaatacg
    19381 gtgctgatgc ccttcgttgg ttcctttcaa acggttccgc accaggtcaa gacgttcgtt
    19441 tctcttacga gaaaatggat gcttcatgga actttattaa caaaatttgg aacatttctc
    19501 gctatatcct catgaacaat gaaggtttga cgctggatgt ggcgcatgac aatgtcacaa
    19561 aagttgcaac aggtgaggct ggtaatgtga cggaccgctg gattctccac aatctcaacg
    19621 aaaccattgc aaaagttact gaaaactttg ataagttcga gtttggtgtg gctggtcata
    19681 tcctttacaa cttcatctgg gaagaatttg ccaactggta tgttgagttg accaaggaag
    19741 ttctatacag cgacaatgaa gacgataagg tcattactcg atctgttctc ctctatacgc
    19801 tggacaagat ccttcgtctc cttcacccaa tcatgccatt cgtgacagag gaaatctttg
    19861 gacaaatctc agaaggttct atcgtgacag ctgcataccc aactgttaat ctagcctttg
    19921 aagaccttgc ggctcacact ggtgtggaaa gtctcaaaga cttgattcgt gctgttcgga
    19981 atgcgcgtgc ggaagtaaat gtagcaccaa gcaagcctat caccatcctt gttaagacaa
    20041 gcgatagcga cttggaagcc ttctttaaca gcaatgtcaa ctatatcaaa cgctttacaa
    20101 atccagaaca cttggaaatc gcatcaacca tccctgcacc tgaactggct atgtcaagtg
    20161 tcatcacagg agcagaaatc tacctgccac tggccgatct cctcaatgtt gaagaagaac
    20221 tggcccgtct cgacaaggaa cttgctaaat ggcaaaaaga actggacatg gttggtaaga
    20281 aactctctaa cgaacgcttc gtagccaacg ccaaaccaga agtcgtccaa aaagaatgcg
    20341 acaaacaagc tgactaccaa gctaaatatg acgcgaccgt cgcacgtatt gatgagatga
    20401 agaagttggt gaaatgatgt taaaaaccta accctatcgg gttgggtttt acatttgata
    20461 atgttataat aaaaaggaga aactttagga gaatactatg gggaaaattg ttgctattga
    20521 tttatttaac ggtgcaggtg gcactacaag tggccttaaa aaatcaggca tagatgttca
    20581 agttgctgtt gaaattgatt ccgtagctgt taaaacttat aagttaaata atcctgaggt
    20641 gtctgtaata gatatggagt aaataaaact tcaattttac aagctttata tggttctcca
    20701 gccaataaaa gtgtgtctga atattggttt agtacaaatg ttgatcaaat agatgaagtg
    20761 gatgggaaac aatgtttaat ctattcatat tataacatta aagcttctag aaatgtagaa
    20821 gttcttaaag gccgttctgg aacaaaaaaa ggattagatt attgggaacc atatgctcct
    20881 caaaaacaat atgagatgga aagattaccc aaaaataaat atattggaag ttcttcgaca
    20941 gacagatggg atggcattga aaaaaatgtt gtcttttgtg attgtaaaga atatgtttct
    21001 gcttttgatt tatttttcta tcactataat tttaaaaaaa tttctactca acgtagtaaa
    21061 caagatttta taagattacg atctaagcca gttgctgata ttttaaagaa taatacaagt
    21121 agctatacgc gttataaaaa ggaaatggtt atagataatg ttaaagttga tgataaagtt
    21181 tgtgaaatta ttagtgaaat aatggatgaa tcttatacag acattcaaat tttaacacat
    21241 aagttgtatt caaaaggtga tgatataaaa gcttccaaaa ctatttggat gaaaaaatct
    21301 ggaaaagaat attccgaggc ttttgcagga acaggtgagg ctagaattat tttgttagta
    21361 aatgatatag taaatgcgca atcaaattca ttaatattaa tagatgaacc tgaaatctca
    21421 cttcatccga gtgcaatcta taaatttaaa gagtttttac ttcaagagtg tttaaataaa
    21481 aaacatcaaa ttattatcac tacacattct acacaactta taaaagattt tcctagagaa
    21541 gccgtgaaac ttttagtgaa aaacggagaa aaggtagatg ttattgaaaa tattgattat
    21601 caggatgcat tttttgaatt aggtgatgtg tatcattcta ggaagatgat ttatgttgaa
    21661 gatagactag ctaaatatat tctagagttt gttatcactc attcaggtag tgagaatctt
    21721 aaacagaatt tagtagtgag atatattcct ggtggagcaa atcaaataat ttgtaataat
    21781 attttaaact catcgtattt agattccgat aaccattatt tttggcttga tggagatcaa
    21841 aacactaatg ttagtgaatc aaataattta atgaactatc ttgaaaatgg tgttgttata
    21901 tcagataaaa ttcctgaatc agataataaa aatcttgatg atattataaa attgataacg
    21961 ggatgtccaa ttaaatttaa tgtttcaggt aataaagggc aaaaaaataa tattgaatta
    22021 attgcgaaac aaagaagctt tatagattat tgggctaaat acgtttctta tcttcctttc
    22081 ccaaccccag agtttttttt agctaatttg tgtaacagcg tagataggga aggttatgat
    22141 ttttctaaag atggcaacgg aaaagaatat tttagaaaaa aaactcaagt agcgttagga
    22201 atagagaata taacttctga agatattttc caggagcaaa gaagagctgt ttcaaaaata
    22261 cagccagaat catcaatgtt tcagtgtatt aaagaaaaat tagaggcatt gttctaattc
    22321 cttagattag gtactacata acaaattttt taaattcaaa cccgcaccaa aaacaacatt
    22381 gcaactttct tctcaggtaa tgtgttggga tttgaacaga ccaacgagtt catagtggtg
    22441 tatgccaatg actttgataa gtctacgcgt cagacttatt agttcattta tccagatata
    22501 tctcgataat catattattt agtcaaagaa catcccatat gagggcatga aatgctaagt
    22561 ttagcttgct cactattaag atgaagtata tatatttcat agaacctatt gtgaaggatt
    22621 tttataattt tcgccaattc catacctatt tcacaattta ccgatgacac agaaaaacaa
    22681 ttgaaaggat atttctatgc aaccaactta caacattgac aatccaaact tgtcttatga
    22741 agcgaagcgt gatttatggc ggataggttt tggtctgcag aaagttgaca atctagtgcc
    22801 atcagcgtat atggaatctt tggctgagaa acagtcccgg ggtgaactga cttatgagca
    22861 ggtttatgag gatgcaacgg cttatcatca taccattgat gcgagtacag aggaggcaga
    22921 cttggtttct ctacgtattg tagaactatt gtctcgaaga ggctttagct tcagtcctgc
    22981 gatcttactt gctattcata aggagttgtt tcaagatata tttgaaccct cgattccggt
    23041 aggtcaattt cgtcagacta atatcacaaa gaatgaacct gttttgaatg gtgaaagtgt
    23101 tgtgtactct gattactcca tgattcaaat gaccttggat tatgatttta atcaggaaaa
    23161 acaagttgca tatgcgacac taacccaggc ggatatggtt aaaaaaatcc agcattttat
    23221 ttcaggaatc tggcagattc atccatttcg cgaaggaaac actcggacgg taacggtatt
    23281 tttgattcag tatcttcgtg agtttggttt tgatattgat aatacaccat ttcagcaaca
    23341 ttccaagtat tttcgtgatg ccttagtgtt agataatgca aagattttac agcgacgtcc
    23401 tgagttttta acagcttttt ttgaaaatct cttgctcggt ggtcaaaatg atttgtcttc
    23461 agaaaaaatg tatctagatt tagacctcga tctttcataa tcctaatact gagtaaacat
    23521 tgaattttag gaaaaaatga agtaaatatt ctcacaagaa aacgtatatc atcaaagttt
    23581 ggctctttgt caattgtagt gggttgaaga aaagctaagt tcgagaaagg acaaatttcg
    23641 tcctttcttt tttgatgttc agagcgataa aaatccgttt ttttgaagtt ttcaaagttt
    23701 cgaaaaccaa aggcattgcg cttgataagt ttgatgagat tattggtcgc ttccagtttg
    23761 gcattagaat agtgtagttg aagggcgttg ataacctttt ctttatcttt gaggaaggtt
    23821 ttaaagacag tctgaaaaat aggatgaacc tgcttaagat tgtcctcgat aagttcgaaa
    23881 aatttctccg gttccttatt ctgaaagtga aacagcaaga gttgatagag ctgatagtga
    23941 tgtttcaagt cttgtgaata gctcaaaagc ttgtctaaaa tctctttatt ggttaaatgc
    24001 atacgaaaag tagggcgata aaaatgttta tcgctgagtt tacggctatc ctgttgaatg
    24061 agtttccagt agcgcttgat agccttgtat tcatgggatt ttcgatgaaa ctgattcatg
    24121 atttggacac gcacacgact catagcacgg ctaagatgtt gtacaatatg aaagcgatca
    24181 agaacgattt tagcattcgg gagtgaaaca gtctgggaga ctgtttcagc ctgagcctag
    24241 aaatttgaaa gcgaagctgt ttagctaagt catagtaagg actaaacata tccatagtaa
    24301 taattttgac gcgacatcgg acggctctat catatttaag aaagtgatct cggatgacag
    24361 cttgtgttct accctcaaga acagtgatga tatcgagctt ttcaaaatct tgcgcaataa
    24421 agctcatctc catctcccga ttgaaacagt cactccccgg actgtttcaa cgtcccaaga
    24481 cataatctta ggcagatttc taaaattaca ctcaaagtgg aagtcattga gctttcgaat
    24541 gacagttgaa gttgaaatgg ccagctgatg ggcaatatcg gtcatagaaa tcttttcaat
    24601 taacttttgc gcaatctttt ggttgataat acgaggaatt tggtgatttt tcttgacgat
    24661 agaagtttca gcgaccatca ttttcaagca atgatagcac ttaaaacgac gttttctaag
    24721 gagaattcta gtaggcatac cagtcgtttc aaggtaagga attttatagg gtctttaatg
    24781 tctagtaatt ttgtgataaa atgtaattgt tccatatgat tctttctaat gagttgtttt
    24841 gtcgcttttc attatagatc ttatgggact ttttttctac ccaaaatagg ctccataata
    24901 tccataggga atttacccac tacaaatatt atagagccca aagttttagg tcgcttgata
    24961 atatgcgttt tttgaatttt atagactgct cgtttaaact ctatttactt cgtaccttct
    25021 ggagcgagac ggaatattag tcacatacaa aatgagtact attaggattt tattttcatg
    25081 tacaatttca gccagtcttg ttataatcag cctataggaa tcaaggaggt gactcttatg
    25141 gctgtttttg tgtctttgga tggaattgtg gtagaagtcc ttgatgtctt ttcttctttt
    25201 aatggggata gtgagttttt cttgtgtata gcattttgaa tctggaatag gacgccatga
    25261 ctgctaaaag atttctataa attaatttga ttttcctaat caatttgttc atatcttatt
    25321 tcattccact ataaacgtct taaagacaag agtcagtttg ttatggaacg ctctcagttc
    25381 gaggagatgt tccaacttca aagtagtcgc ttgacgacgc aagaaaaatt acaattgttt
    25441 acctctgtgt ttgctggccg ttatgatgtt tatgctaaga attttatcaa tgaacaaggg
    25501 aaaattcagt attttccttc ctatgattat ggttggaagc agttgccacc tgaaaaacgg
    25561 agtttccaga cattgacgaa ctccgttttg aaatctcatt ttcgtgggga ggcagctatc
    25621 ggtatctttc ctatgcactt agatgatagc tgttattttt tggtactgga tttggatgaa
    25681 ggagattgga aagaagctgg tttaaccatt cgaagaatag ccagggaacg ccagatggaa
    25741 gcccatttag agatttctcg ttcgggtcac ggactccata tttggttctt ctttgaggaa
    25801 gcgattccga gtcgagaggc tcgcttgttt ggaaagaaac tgatagaact ggcaatgcag
    25861 gaaagtatgc aactgtcctt tgattctttt gatcgcatgt ttccaaatca ggatgtcctt
    25921 cctaaggggg gatttggaaa tttgattgcc ttgccttttc aaggagaagc ttaccatcaa
    25981 gggcgaacgg tctttgtgga tgaacagttt cagccttatg aagaccaatg gaggtatcta
    26041 caagaaattc agaggatttc aactgctaaa gtggcactgt taatccaaga ggagttaggc
    26101 aagcaagaat tggataagga gttgaaggtc gttttatcca atatgatcca acttgaaaaa
    26161 tcgtctgtga catccaaggc acttttttct tgaaaaatat ggcttccttt tctaatcccg
    26221 aattttatag tagattgaaa ctagaatagt acacctctgc ttctaaaaca ttgttagaaa
    26281 tcgatttgac tttcctgatc gatttgtcct gttattattt cattttacta tatttaaagc
    26341 aggctatgcg acagccaacc tatcaaattc ctgagagaat gtatttattt ggagaatccg
    26401 atcattattt atggttgcca agaggtttgc tgtatccatt gcaagataaa tttaagcagg
    26461 tatctgtgga agataggaga aaggtacaaa ggtctattag cgtggaattt aagggagaac
    26521 tcacttttga gcaagagtta gccctgtcag atatgacttc taaagaaaat ggtttacttc
    26581 atgcggagac tggttttggg aagaccgttt taggtgctgc tcttatctct gaacggaaaa
    26641 caaaaacaat tattctagtc cataataagc aactcttaga ccaatggcta gatcgcttaa
    26701 actgcttttt gactttcgaa gaggaggagg ctatccgtta tacggcatca ggtcgtgaaa
    26761 aggtaatcgg ctatgttggg cagtacggtg ggactaagaa atggctgagt aaactggttg
    26821 atgtcgttat gattcaatct ctatttaagt tggaaaatag tcaaagtctt ttggatgagt
    26881 atgagatgat gattgtggat gagtgtcatc atgtctctgc cttgatgttt gaaaaagttg
    26941 ttgctcagtt tagagggaag tatctttacg gtttgacggc tacgcctgag cgtaagaatg
    27001 gtcatgagcc tattgttttt cagagaattg gtgagatact ccatactgct gataagaggg
    27061 aaacggattt taaacggcaa ttgca
//
LOCUS       DAVHEX010000026        27079 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVHEX010000026 DAVHEX010000000
VERSION     DAVHEX010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27079
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.026"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..710
                     /locus_tag="VVL45_001080"
     CDS             <1..710
                     /locus_tag="VVL45_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU4187266.1"
                     /translation="GRTQAIIRNHFLKYDRAVRCRVKIITMDMFSPYYDLARQLFPCA
                     KIVLDRFHIVQHLSRAMSRVRVQIMNQLDRKSHEYKAIKRYWKLIQQDSRKLSDKRFY
                     RPTFRMHLTNKEILDKLLSYSQDLKHHYQLYQLLLFHFQNKKPEKFFGLIEDNLKQVH
                     PIFQTVFKTFLKDKEKIVNALQLHYSNAKLEATNNLIKLIKRNAFGFRNFENFKKRIF
                     IALNIKKERTKFVLSRA"
     gene            complement(782..1432)
                     /locus_tag="VVL45_001081"
     CDS             complement(782..1432)
                     /locus_tag="VVL45_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEU4187267.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            1544..2386
                     /locus_tag="VVL45_001082"
     CDS             1544..2386
                     /locus_tag="VVL45_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU4187268.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADARQIGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            2462..2899
                     /locus_tag="VVL45_001083"
     CDS             2462..2899
                     /locus_tag="VVL45_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEU4187269.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            2968..3271
                     /locus_tag="VVL45_001084"
                     /pseudo
     CDS             2968..3271
                     /locus_tag="VVL45_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3362..4435)
                     /locus_tag="VVL45_001085"
     CDS             complement(3362..4435)
                     /locus_tag="VVL45_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEU4187270.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPREPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSRQVDT
                     TFKEWVTTVPDEELQLYFDLFFGTILDAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     EKETLGRLTQLLIDTRYQVWKNR"
     gene            4591..5268
                     /locus_tag="VVL45_001086"
     CDS             4591..5268
                     /locus_tag="VVL45_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4187271.1"
                     /translation="MHKILLIEDDQVIRQQIGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            5261..6235
                     /locus_tag="VVL45_001087"
     CDS             5261..6235
                     /locus_tag="VVL45_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEU4187272.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVETQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLVLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(6284..8227)
                     /gene="thrS"
                     /locus_tag="VVL45_001088"
     CDS             complement(6284..8227)
                     /gene="thrS"
                     /locus_tag="VVL45_001088"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEU4187273.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFEKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(8285..8608)
                     /locus_tag="VVL45_001089"
     CDS             complement(8285..8608)
                     /locus_tag="VVL45_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEU4187274.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDSKWTVEEDPLALSNDIGN
                     FQRLVMTKQGRYYDETPYTLEQKLSENIWWLLELSQRLDIDILTEMENFLSDKEKQLN
                     VRTWK"
     gene            complement(9039..9239)
                     /locus_tag="VVL45_001090"
     CDS             complement(9039..9239)
                     /locus_tag="VVL45_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187275.1"
                     /translation="MRRFYSHLPYYLVILFFYWPLYELFLLVVSDPLTLKELYINNLL
                     FFTPLVILIVSLLYSYRFRFSL"
     gene            9477..10106
                     /locus_tag="VVL45_001091"
     CDS             9477..10106
                     /locus_tag="VVL45_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU4187276.1"
                     /translation="MKLAVIAANGQAGKAIVEEVVKRGHEVTAIVRSENKSQSESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGAGS
                     LYIDETKTTRLLDTSDFPEEFKSLAKAQADELDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKGISQISYADYAIGLVDELEKGHHIKERISLLEK"
     gene            10247..10516
                     /gene="rpsO"
                     /locus_tag="VVL45_001092"
     CDS             10247..10516
                     /gene="rpsO"
                     /locus_tag="VVL45_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEU4187277.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            11331..11945
                     /locus_tag="VVL45_001093"
     CDS             11331..11945
                     /locus_tag="VVL45_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEU4187278.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(12237..12986)
                     /locus_tag="VVL45_001094"
     CDS             complement(12237..12986)
                     /locus_tag="VVL45_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEU4187279.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            13119..15455
                     /locus_tag="VVL45_001095"
     CDS             13119..15455
                     /locus_tag="VVL45_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEU4187280.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAVASILTSYMA
                     HSEDKNPTAQAIRQRFVGDVAYPMISNLPFSSDRKWGAMELEGLGIVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLDY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHNYVDCSKITDEELMAMAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLIRSMLRALPSALMVVFSVLFVKIF
                     GTSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTEQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            15557..16234
                     /locus_tag="VVL45_001096"
     CDS             15557..16234
                     /locus_tag="VVL45_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEU4187281.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            16221..17018
                     /locus_tag="VVL45_001097"
     CDS             16221..17018
                     /locus_tag="VVL45_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEU4187282.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSCGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQEDDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIATLLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            17035..18138
                     /locus_tag="VVL45_001098"
     CDS             17035..18138
                     /locus_tag="VVL45_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU4187283.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQEIDFYQRSGVFLLKKDESNLE
                     ELYQLALQRREESPLIGQLAILNQASANELFPGLQGFDRLLYASGGARVDGHLLVTRL
                     LEASQVKLVKEKVTLTLLSSGYQIGEEVFDQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLAQDMEDYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDEPLLQQME
                     EAALPHYPVLARVTSRAERVGIRAYTSDFSPFFGQVPELAGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPENYPIENYVKRVKSE"
     gene            18145..18336
                     /locus_tag="VVL45_001099"
     CDS             18145..18336
                     /locus_tag="VVL45_001099"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187284.1"
                     /translation="MKIKEQTRKLAAGYSKHCFEVVDRTDEVSSKHCFEVVDRTDEVS
                     NHTHGKATLTWFELDFRRV"
     gene            18439..19458
                     /gene="galE"
                     /locus_tag="VVL45_001100"
     CDS             18439..19458
                     /gene="galE"
                     /locus_tag="VVL45_001100"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU4187285.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTDTLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            19460..20425
                     /locus_tag="VVL45_001101"
     CDS             19460..20425
                     /locus_tag="VVL45_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU4187286.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIINFSDFPLIIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKNKVKRL"
     gene            complement(20476..20682)
                     /locus_tag="VVL45_001102"
     CDS             complement(20476..20682)
                     /locus_tag="VVL45_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEU4187287.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            20734..21210
                     /locus_tag="VVL45_001103"
     CDS             20734..21210
                     /locus_tag="VVL45_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEU4187288.1"
                     /translation="MAKEPWQEDIYDQEESRAERRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSHPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            21219..21890
                     /locus_tag="VVL45_001104"
     CDS             21219..21890
                     /locus_tag="VVL45_001104"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEU4187289.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPQVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            22021..22359
                     /locus_tag="VVL45_001105"
     CDS             22021..22359
                     /locus_tag="VVL45_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEU4187290.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLTDGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            22362..22991
                     /locus_tag="VVL45_001106"
     CDS             22362..22991
                     /locus_tag="VVL45_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEU4187291.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITRHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            23231..24382
                     /locus_tag="VVL45_001107"
     CDS             23231..24382
                     /locus_tag="VVL45_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4187292.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            24700..25449
                     /gene="truA"
                     /locus_tag="VVL45_001108"
     CDS             24700..25449
                     /gene="truA"
                     /locus_tag="VVL45_001108"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEU4187293.1"
                     /translation="MTRYKATISYDGYAFAGFQRQPHARSVQEEIEKTLTRLNKGQAI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFTVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            25439..26212
                     /locus_tag="VVL45_001109"
     CDS             25439..26212
                     /locus_tag="VVL45_001109"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU4187294.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LVKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            26190..26651
                     /locus_tag="VVL45_001110"
     CDS             26190..26651
                     /locus_tag="VVL45_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU4187295.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSALMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
     gene            26879..>27079
                     /locus_tag="VVL45_001111"
     CDS             26879..>27079
                     /locus_tag="VVL45_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEU4187296.1"
                     /translation="MKLTYDDKVQIYELRKQGYSLEKLSNKFGINNSNIRYMIKLIDR
                     YGIEFVKKGKNRYYSPDLKQEMI"
BASE COUNT         7692 a         5084 c         6158 g         8145 t
ORIGIN      
        1 agggaagaac acaagctatc attcgaaatc actttcttaa atatgataga gccgtccgat
       61 gtcgcgtcaa aattattact atggatatgt ttagtcctta ctatgactta gctagacaac
      121 ttttcccatg tgctaaaatc gtgttggatc gctttcacat tgtacaacat cttagccgtg
      181 ctatgagtcg tgtgcgtgtt caaatcatga atcaattgga tcgaaagtct catgaataca
      241 aggctatcaa gcgctactgg aagctcatac aacaggatag ccgtaaactg agtgataagc
      301 gattttatcg tcctactttt cgtatgcact taaccaataa agagatttta gacaagcttt
      361 tgagctattc acaagacttg aaacaccact atcagctcta tcaactcttg ctgtttcact
      421 ttcagaataa gaaaccggag aaatttttcg gactcattga ggacaatcta aagcaggttc
      481 atcctatttt tcagactgtc tttaaaacct tcctcaaaga taaagaaaag attgtcaacg
      541 cccttcaact acactattct aatgccaaac tggaagcgac caataatctc atcaaactta
      601 tcaagcgcaa tgcctttggt tttcggaact ttgaaaactt caaaaaacgg atttttatcg
      661 ctctgaatat caaaaaagaa aggacaaaat ttgtcctttc tcgagcttag ctgacttcaa
      721 cccactacag ttgacaaaga gccaaaataa aactttcagg gttggtaggg gacttgagaa
      781 attagttgat tttctcgaca tagagttgtt ttgcaatgtc catattcact tgtaaatcct
      841 cgtcgttact gaggatagtg aaggtattgc tgaagccatc aaactgcttg acttggagct
      901 ggtcaccgat gtgaagtgag tgcttgtcca gataatggag aatgtcaaaa ctatcgtgca
      961 cccgagtcag gcggtaggcg ccagcttcct tgatatcagc tagtgggagg ttattgattt
     1021 caacgagtag ttctcccttg gcaggaatag ttcccccgtg ggggcaggtt ttagggaaac
     1081 ctagcagttt atctagtctt tccacgaaca ggtcagagac agtgtgttcc aagacctcag
     1141 cttcctcgtg aatctggtca cttgtatagt ctaaatgatg aactagaaaa acttcaatca
     1201 agcggtgctt acgatagagc tcagagacca gtttgagacc gaggtcagtc agtagatagc
     1261 cacattcctt gtcctttagg atgagatttt cacttttcat tcgtttgatc atttcagtta
     1321 cggcaggggg agagacttgc atgcgagccg caatttcctt gttggtaatc ttatgcaggt
     1381 ctatgccaat ttcataaata cattttagat agtcttcttt gtttggggtc attcgtttcc
     1441 tcttgtctaa tatctttatc tagtatatca aaaaaagcta gatttctcta gctgtgactt
     1501 tttatgttat acttattgca agagaaaaaa cgaggagatg gatatgacga aaatagctct
     1561 tctttcagat attcatggaa ataccaccgc cttggaggct gttttggcag atgctcggca
     1621 gataggagtg gatgaatact ggcttttggg agatattctc atgccaggga cagggcgtag
     1681 aaggattttg gacttgttgg atcaactacc gattacggct agagttttgg gaaactggga
     1741 agacagtctt tggcatggtg tccgtaagga attggatagt actcgcccca gtcaacgcta
     1801 tctcttgcgc cagtgccagt atgttttaga ggaaatttcc ctagaagaaa ttgaagtgct
     1861 ccacaatcaa cctcttcaaa ttcatcgtca gtttggggat ttgacggtgg gaattagcca
     1921 tcatctgcct gataagaact gggggcgaga gttgattcat actggcaaac aagaggagtt
     1981 tgaccgcttg gtgactcatc ctccctgtga tattgctgtt tatggacata ttcaccagca
     2041 gttgcttcgt tacgggactg gtgggcaatt gattgtcaat ccgggttcga ttgggcaacc
     2101 tttctttttg gacgctcagc tacggaagga cttgcgggcc cagtatatga ttttggagtt
     2161 tgatgacaaa ggcctggtag atatggactt ccgacgggta gactacgatg tggcagctga
     2221 attgcagctg gctaaagacc tgagacttcc ctattttgag gtttactatg aaagtctggt
     2281 caatgggatc caccatactc atcatcagga atttttgaga gaattggctc agaaagaggg
     2341 ctgcgatcgg gagttagacg actggttgaa aagtggtaac gattgacatt acaactaaaa
     2401 agggtataat aatagaaaaa gaagagtaga ggcagggtag cctcgtaaaa aggagaagag
     2461 gatgcaaatt ccaagtagat ttaccattgc gactcatatg ctgataatca ttgccctcga
     2521 agggaaggaa agcaaggtga ccagtgattt tctggctgct agtgtcgggg tcaatcctgt
     2581 cattatcaga aagatcttgt cccagttgaa gaaggcagag ctgatttcag tagcgcgtgg
     2641 aacgggcgga acagagattg tcaaggacct taaggatatt agtcttttag atgtttatca
     2701 ggcggtagaa tgtcttggta agacaggtca actcttcagt ttccatgaca atccgaatcc
     2761 aaattgccct gtaggagctc atattcatga tgttttggat caaaaattgg agagaattca
     2821 gttgactatg gaggcagaac ttggtcaaac cagtctagaa aaagtcgtgg ccgatgcaga
     2881 gagtcagatg aaggattaag agggataaat gccctctttt tttctttgga taattatgat
     2941 aaaatgtagc tattaaagga gtaagaaatg tatctcgtta taggaattgt attagccttt
     3001 atagtgtcat tttggaagta taatagaagt ttgtggaatc cagtattatt tttattatcc
     3061 ctcatttcaa gttattttta tctatcctac cttttttata aaaatggata cgagaatgtt
     3121 cagttagctt tttatgtatt tgcctttgta ttacttcctt tttttgcttt ttctaagtgg
     3181 catcttttta atctataatg gggtcatttt gttgaagcga gagggacgtt ccaagcccca
     3241 ttatctctca atgctctttg atttttattg actattagat tctatttaaa aagacttaga
     3301 atgcacatga aagtatgtat tctaggtctt ttgttttgta tacatttaat tttcaagagt
     3361 attatctatt tttccatacc tggtaacgag tatcaatcaa taactgggta aggcgaccca
     3421 aggtttcttt ttcttctgga gtgagggatt gagcttggac aaagagatga tgaatgtatt
     3481 caagcgcctt taaggaagcc aagtcattga tagagctaat accagcatca agaatagtgc
     3541 caaagaagag gtcgaagtag agctgaagtt cttcgtcagg gactgtggtc acccattctt
     3601 taaaggttgt gtctacttgc cggctatcac tgttggtctt atccagttga acgaagtgct
     3661 tatcctcaat ctgccaacta aaggtatcgt gctgggcgat gccacccagg gcagtactct
     3721 gaacgatgat ttggtgagca ggaatttcca gcatcatacc gataatggaa ccttgtggaa
     3781 tgaatatctt gcttctatcc attatccttt gataacccgc agtctgtgtc aattcttgat
     3841 ggagaccagg tgcatcaaat gtataaactg ctgtgatctg attttgcaaa ctttgctcaa
     3901 tttggctagc agcatagata gcgagatttc ctcccttgga atgcccagcc agaataacct
     3961 tttgcttagg atgatgggca aaaaagttct ttaaatagcg aagggcgtgc ttttgagcag
     4021 gaatttcctt catataggtc aggtggaaat cttccttcca gccaatgata ctgtcatctg
     4081 tcccacgaaa gacaatcaga taggtatcga ggctgacacg ataagtcata gccgcaaatt
     4141 gcttttgcag ttcagggtcg atgtcgttga taaaatggga gagtttgcaa tttttgaagc
     4201 gcttgtgttg agccaattca tctaataatt gaaggcgatt tttgctagta agcatggtgg
     4261 gctctcttgg aacctgaggt gctagatcta aaagacgttg aggaagtgtg gagaccagat
     4321 tatcaaagga gaggtaggtg atttctatta aggttagaat gtctaactca ttcaagggaa
     4381 ggtcgtaata agaatcatat gcgacatctt tcagatagtc aaaaatattg gccataagag
     4441 ctcctttctt tttatttatc ttagcaaatt tccctcaaat tagctagtag catagcctgt
     4501 ttgtactggc taaaaacagg ctatttcaaa ttcagtttca gaccatctag catggaaaaa
     4561 tctgttataa taatggaaaa ggagaagcgc atgcacaaga ttttattaat agaagatgat
     4621 caggtcattc gtcaacagat tgggaaaatg ctctctgaat ggggatttga agtggtcctg
     4681 gtagaagact ttatggaagt tttgagtcta tttgttcagt cggaacctca tctggtcctc
     4741 atggatattg gtttgccctt gtttaatggt tatcactggt gtcaggaaat ccgcaagatt
     4801 tccaaggtac ctatcatgtt tctttcttcg agagaccagg ctatggatat tgtcatggca
     4861 atcaatatgg gggcggatga ctttgtgacc aagccttttg accagcaggt tcttttagct
     4921 aaggttcagg gcttgttgcg tcgttcctat gagtttgggc gtgatgagag tttgctggaa
     4981 tatgctggtg ttatcctcaa taccaaatcc atggatttac attatcaagg gcaagtcttg
     5041 aatttgacca agaatgaatt ccagatttta cgcgtgttat ttgagcatgc aggcaacatc
     5101 gtagcacgtg acgacctgat gcgggaactt tggaacagtg actttttcat tgatgataat
     5161 accctctctg tcaatgtggc tcgtttgcgt aaaaagttgg aggagcaggg attggtagga
     5221 tttatcgaga ccaagaaagg aatagggtac ggattgaagc atgcttgatt ggaaacaatt
     5281 ttttctagcc tatctgcgct cccgtagtcg tctttttatc tatctgcttt ctttggcatt
     5341 tcttgtctta ctctttcagt ttttatttgc cagtctagga atttacttcc tctacttttt
     5401 cttcttgtgt tgctttgtaa ccatattatt tttcacttgg gacatattgg tggaaacgca
     5461 ggtctatcgc caggaacttc tctatggaga gagggaagcc aagtctcctt tggaaatagc
     5521 tttagcagaa aaattagaag cgcgtgagat ggaactctat cagcagaggt caaaagcaga
     5581 aagaaaactg acggatttgc tggattacta taccttgtgg gtccatcaga taaagacccc
     5641 cattgcagcc agtcaactct tagttgcaga agtggtcgac cgccaactga agcagcagct
     5701 agaacaggaa attttcaaaa tcgactccta taccaaccta gttttacagt acctgcgttt
     5761 agaaagtttc catgatgatt tggtcttaaa gcaggttcaa attgaggact tggtcaagga
     5821 aataattcgt aaatatgctc ttttctttat tcaaaaaggc ttaaatgtca atctacatga
     5881 ccttgataaa gaaatcgtga cggataaaaa gtggctgcta gtggttattg agcaaatcat
     5941 ctcaaacagt ctcaagtaca ccaaggaagg tggtctggag atttatatgg atgaccaaga
     6001 gctttgtatc aaagatacgg gaatcgggat aaaaaacagt gatgtcctcc gagtatttga
     6061 acgtggcttt tcaggataca atggccgttt gacccagcag tcctctggac ttggccttta
     6121 tctatctaag aaaatttctg aagaactggg gcaccagatt cgtatcgagt ctgaggtcgg
     6181 aaaaggaacg acagtgcgga ttcagtttgc tcaagtgaac ttagtccttg agtaaaaata
     6241 gatgagaaaa agcctccaga ttggaggctt ttatgctaga ctcttatttc tcaacgcgtg
     6301 atttgttggc gatatcagct aggatagctt gaacaaaatt atcaactgag acagtttgtg
     6361 tttctttttg gccgtagcga cgaacgttga ctgtttcgtc ttccatttct ttgtctccaa
     6421 caattaattg gtaaggaatc ttgctggttt gtgaagcacg gatcttgaac tgcatttttt
     6481 cattgcgctc atctacgtct gcacggacac cgcggtcacg gagtttcttg gctacttccc
     6541 aagcgtagtc cacgtgtttt tcgttagata ctgggataag ggttacttgg tgtggtgcca
     6601 gccatgttgg gaaggcaccc ttgtagttct caatcaagat agctgtgaag cgttccatag
     6661 ttgagataac cccacggtgg atcatgactg gacggtgatc ttcgccatca gctccgatgt
     6721 atttgaggtc gaagcgttct ggcaacaaga aatcaagttg gatagtagaa agggtttctt
     6781 cttttccaag ggcagtttta atctggatat ccaattttgg tccgtagaag gccgcttcac
     6841 cttcggcttc aaagtagtcc acgcccattt catcaagagc tgcacgaagc atggtttggg
     6901 cattttccca catctcatcg ttatcaaagt acttatgagt atcttgaggg tcacgaagag
     6961 agaggcggaa gcggtagtca gtcaagttga agtcttcata aacatcgata atcaactgaa
     7021 gggcacgttg gaattcttct tggatttgtt ctggagtaac gaataggtga ccgtcgttga
     7081 gtgacatttc acgtacacgt tgaaggccag tgagggcacc agatttttcg taacggtgca
     7141 tcataccgat ttcagcgata cggattggca attcacggta agagtgaaca tggtgtttga
     7201 aaacttggat gtggtgcgga cagttcattg gacgaaggac aaattcttcc ccgtcaccca
     7261 tgtccatggt tgggaacatg tcttcttggt aatgatccca gtggccagaa gtcttgtaaa
     7321 gctcaacaga agcaagtggt ggagtgtaga cgtgttggta gccagaagcc aactctttgt
     7381 ttacgatgta gcgttccaat tcacgacgga tagtcgcacc atttggcaac cagaatggca
     7441 aaccttgtcc cacttcttgt gaaatcataa agaggtcaag ctctttacca agtttacggt
     7501 ggtcacgttc cttagcttct tcacgcattt gaaggtagtt tttcaagtct ttcttttcaa
     7561 accaagctgt accgtagata cgttgcatca tagcgttgtc gctgtttcca cgccagtacg
     7621 caccagctac atggagaagg tggaagattt ggatacgacc tgttgatgga acgtgaggtc
     7681 cacggcagag gtctacatat tcaccctgac gatagatagt caaaccgcct tcgtcttctg
     7741 agtgttcttc aatcaattcc aacttgtaag ggtcattttt gaagatttca cgtgcctcgt
     7801 ctttagtcac ttcttcacga atagatggga agttttcttt gacgattttt tgcatttctt
     7861 cttcgatacg aggaaggtct tcgttagaga tttgaccagc tgtgttgtca gtatcgtagt
     7921 agaaaccatc ttcgatggct ggaccaactc ccaagtgaat gtctgggaaa agacgacgag
     7981 ctgcttgggc gaacaagtga gctgctgagt ggcgcaagat tggaagggca tcttcgtgat
     8041 caggtgtcac aatttcgatg cttccatctt cagtgatagc gcgagtagtg tcgatgagtt
     8101 tgccgttgaa tttaccagcc aaggcttttt tagctaggga attgctgata gattgggcaa
     8161 tttcaaaagt tgttacgcca gattcgaatt cacgaacagc gccatctggg aaagtaatgt
     8221 taatcatgat gttctccttt tttattattt catagtttct aagcattgat tttttatcat
     8281 cagactactt ccaagtccta acgttcaatt gcttttcttt atcagagagg aagttttcca
     8341 tttccgtcag aatgtctata tccaaacgtt gagaaagttc taatagccac cagatatttt
     8401 ctgaaagttt ttgttccagt gtgtagggtg tttcatcata gtagcgtcct tgctttgtca
     8461 tcaccagtcg ttggaaattt ccaatatcat tagataaagc caaggggtct tcttctaccg
     8521 tccacttgga atcatgatgc ttaacttcca gttcgtgata agcttgtcgg attgcccaac
     8581 tgcgctccac taattcttgc aactccataa ttagtatcct aataaaaaaa tgcgacatcc
     8641 tcgaaaggac gtcgcaacgt ggttccacct tcatttatgt acctcaaaag agagggacac
     8701 ctctgattgg ctctaacgtg gccaccgttt tatgttttca taaaaaactc aagagtagta
     8761 tcagtttaga cttcctatgc gtttccagca accacgcact ctctagtaga aagggactaa
     8821 gtgacttgtc tctatggatt attatagcat gattgtgaga tttttcaagg attttgtgaa
     8881 agttttttgt ttttcatttt ttgtagaata tgcttgatgc taataccaga atccatgccc
     8941 accagagcaa ctgtttcata gattagcaag taaattagta taaactcacc aaaggttatg
     9001 atagtaaagt aaaagagcag tccgttacca actaaccatc aaagtgagaa acggaaacgg
     9061 tagctataga gtaacgatac aatcaagatt accagaggtg taaagaagag aagattgttt
     9121 atatagagtt ccttgagtgt aagggggtca gaaacaacta gtaagaacaa ctcataaagt
     9181 ggccaataaa agaataatat gaccagatag taggggagat gggaatagaa tctacgcatg
     9241 ataatcacct cgttttaaaa aaacagacaa cttagatctc ttgctatgat tattatagca
     9301 tgtttaaaac aagaaagtaa atttgttggc aaagaagatt tttttttcac tagaatcatg
     9361 gttagttttc ttggtagaaa tattttgata ataattaagc aaaactattg acattgatta
     9421 ttaagagtgt cacaattgtt gtaactaaaa taattacaat aagagaggta taaaaaatga
     9481 aactagcagt tattgcagca aatggacaag cggggaaagc aattgttgaa gaagtagtca
     9541 aacgaggaca tgaagtaacg gctattgttc gttctgaaaa caaaagtcaa tctgagtcta
     9601 ttattaaaaa agatttattt gagttgacaa aagatgatct tactggattt gatgctgtta
     9661 tttcagcgtt tggtgcttat acaccagata cgctaccgct ccacagcaaa tcaatcgaac
     9721 tatttaatca gcttcttgct ggaacacaga cacgttttct agttgtaggg ggagcaggaa
     9781 gtctttatat agatgaaaca aaaacgacac gacttttaga tacgtctgat tttcctgaag
     9841 aatttaagtc actagcaaaa gctcaagcgg atgaattgga tcttcttcgt acaaaaaata
     9901 atctcaactg gacttttgtc agtcctgctg tagattttat tcccgatggt gaaaaaacag
     9961 gaaactacat tctagcagga gaaattttta ctactaatga aaaaggaata agccagatta
    10021 gttatgcaga ttatgctatt ggattggtag atgaacttga aaaaggtcat catataaaag
    10081 aacgtatctc tttacttgaa aaataataac tatgagagta ttaatgtttg acagaaaatt
    10141 taaatcttgg taaaataggg attgtcgtaa agacaaataa cttcttcttg gttacaggca
    10201 tgccaacctg tcactcggat gaagccaaat aaaaaggaga aacatcatgg caatctcaaa
    10261 agagaaaaaa aatgaaatca ttgcacaata tgcacgtcac gaaggtgata caggttcagt
    10321 agaggttcaa gttgctgtcc ttacttggga aatcaaccac cttaacgaac acatcaaaca
    10381 acacaaaaaa gaccacgcta cttaccgtgg attgatgaaa aaaatcggtc gccgtcgtaa
    10441 cttgcttgca tacttgcgta aaaacgacgt taaccgttac cgtgagttga tcaactctct
    10501 aggacttcgt cgctaattca agatacaaag gccgtcaaaa acacaaagca aaaataggaa
    10561 aattgacgaa gaaacttcag tttctaggag attttatctt ttttgccaag tgcttaggcc
    10621 gtgttcaatt gagcatatct tgataataaa gctactctaa attgggtagc tttgttgcgt
    10681 tgtcacctta cctcgctata ctcaagtata atcttcggtt acggttccta gcactgtaag
    10741 gtaaaataaa ctagcttttc tcccttcggg gagatttttt tgtttcactg aaagtttcgt
    10801 acgatcttca cctcgtcccc tagtctataa acgatttatc tagcaagaat catgatgcta
    10861 agggcgtcaa aaatccgtat gaaaataggg aaatgacaca gtgttcgatg agcacaagga
    10921 gtttcatctt tttcactagg attttagccc gggctaaaat cagctctctg acttcagagg
    10981 gcttttttat tgaggtttta tcggtaacaa ttttggagac tacaaaaacc tcaaatggta
    11041 tcagctaatt acaccataaa ggtgcgcagt tactcggctt ttcaagccga gtagctgtat
    11101 gcaagccccc tagaagagcc cactctttac aaagtaaagt atagtatgtt atactttaca
    11161 tggaagtagt cactgaattc cagttagaaa ttactttgta actacgtttt gaggaggagt
    11221 aaaatgcttt cctacgttcg acattaccca ctagcgatag ctaaattaat gtgtctgtgc
    11281 tctcctaaaa tctgctgatt tattactgac taatacagga ggtttttttt atgggacaga
    11341 caatcatatc tgctattggt gtttatattt ccaccagtat cgattattta attattttaa
    11401 ttattttatt tgcacagcta tcacagaata aacagaaatg gcatatttat gcggggcaat
    11461 atctaggcac aggcttactt gtaggggcga gtttagttgc tgcttatgtc gttaatttcg
    11521 tgcctgaaga atggatggtt ggattgcttg gtttaatccc tatctattta gggattcgct
    11581 ttgcaattgt tggagaagat gcggaagaag aagaggaaga aattattgaa agattagaac
    11641 aaagcaaggc aaatcaactg ttttggacag ttacattgct gacaattgcg tctggcggag
    11701 ataatttagg tatctatata ccttattttg cttcgttaga ttggtcacag accctcgtgg
    11761 ccttgcttgt gtttgtaatc ggcataatta tcttttgcga gattagtcgg gtgttatcct
    11821 ctattccgtt aatattcgag acaattgaaa aatacgagcg aatcattgtg cccttagtat
    11881 tcattctact tggactatac atcatgtatg aaaatggcac gatagagact tttctgatcg
    11941 tgtagatttt tttgtttcac taggatttta gcccgagctc aaatcagctc tctgattttc
    12001 agggggcttt ttcattgagt ataaatggac aatcttaaag ttactctatt gtagagtggc
    12061 ttttgatttg attaggagat tcttataaat atagtaaaat gaaataagaa taggacaaat
    12121 cgatcaggac agtcaaatcg atttctaaaa atgttttaga agtagaggtg tactattcta
    12181 gttccaatct actatataaa tgaaaacctt agctgtttgg ccaaggttca tttcttctat
    12241 gcaagttctt ctcttttctt atctgggttc cagatgaagc ttgcgataaa gctaaagatg
    12301 atggttagga tagcgaggat aatagcaagg atgagggcag ggatgcggat aaaccaccag
    12361 agtggatttg gttttttatc attgtgccat tgtttcgttt cttcgtccag acgttggaat
    12421 tctgtttgaa tacgattggc gactgtttca atgccttcat cattcatttt cttgatatct
    12481 gagatatcga ttggatttcc aaagttcata tcgacacgtt cacggctaat caagcccttc
    12541 aaagtcatgg gaccggtgta ggtaaccggc atgatacgga ccttggccat tttggcaatc
    12601 agtgctgcgc cccccttgac atcgtttgag tggcggctac cacttggaaa catgatgaga
    12661 gagcggtcac tttttttgag aacgttgata ggatatttga tggctgaggc gctgggattt
    12721 tcacggtcga tgggaaaggc gccacacata cgaatccacc aaccaaagat acggttggta
    12781 aagagttctt tttttgccat aaagatgaac tgttttggct tggtcgcaaa ggccatataa
    12841 acaggatccc accaggtacg gtgaggcgca actaaaatat aattttcatc ttgattagga
    12901 attttatcag tattatgata gtgagcattg ccattgatgg accataagag caatacaact
    12961 aatccacgca aataagtata aaacatgcga tctccttcaa ttgttttctt gttattatta
    13021 taccttatca aaggagggct ggcaaacttt tcccttgact agatgcatat ttgggatgaa
    13081 attagaattc tgttaaaaaa aatgatataa tagaatttat ggataaaaat aagattatgg
    13141 gattaaccca aagagaagtc aaggaaagac aggctgaggg tttggtcaat gactttaccg
    13201 catcagccag taccagcact tggcaaatcg ttaaacgaaa tgtctttacc ctttttaacg
    13261 ctttgaactt tgccattgct ttggcccttg cctttgtgca ggcttggagc aatctggtct
    13321 tctttgctgt tatctgcttt aacgcttttt ctgggattgt gaccgagcta cgagccaaac
    13381 acatggtgga caagctcaat ctcatgacca aggaaaaggt caaaaccatc cgtgatggtc
    13441 aggaagttgc tcttaatcct gaagaattag tgctaggaga tgtcattcgt ttgtctgcag
    13501 gagagcagat tcctagtgat gccttggttt tggaaggctt tgcggaagtc aatgaagcca
    13561 tgttaacggg agaaagtgat ttggtgcaaa aggaagttga cggcttactt ttgtcaggaa
    13621 gtttcctagc cagtgggtca gttttatctc aagttcacca tgtcggtgca gacaactatg
    13681 ctgccaaact catgcttgag gctaagaccg ttaaacccat caactcccgt atcatgaaat
    13741 cgctggacaa gttggctggt tttactggga agattatcat tccctttggt ctggctctct
    13801 tgctggaagc cttgctttta aaaggcctgc ctctcaagtc atctgttgta aactcgtcga
    13861 cagctctttt gggaatgttg cctaagggaa ttgccctttt gaccattact tcgctcttga
    13921 ctgcagtgat taagctaggt ttgaaaaagg tcttggtgca ggagatgtac tctgttgaga
    13981 ccttggcgcg cgtggatatg ctctgtctgg acaagacggg taccatcacc caaggaaaga
    14041 tgcaggtgga ggctgttctt ccactgacgg aaacttatgg tgaagaggct gttgccagca
    14101 tcctgaccag ctatatggcc catagtgagg ataaaaatcc aacagctcaa gccattcgcc
    14161 agcgttttgt gggagatgtt gcttatccta tgatttccaa tcttcccttc tcgagcgacc
    14221 gcaagtgggg ggctatggag ttagaaggtt tgggaatagt tttcttaggg gcacctgaga
    14281 tgttgctgga ttctgaagtc ccagaagcca gggaggcctt ggagagagga tcacgtgtct
    14341 tggtcttagc tctcagtcag gagaaattag accatcacaa accacagaaa ccatctgata
    14401 ttcaggctct agccttgctg gaaatcttgg accccattcg agagggagca gcagagacgc
    14461 tggactatct ccgttctcag gaggtgggac tcaagattat ctctggtgac aatccagtta
    14521 cggtgtccag cattgcccag aaggctggtt ttgcggacta tcacaactat gtagattgct
    14581 caaaaatcac cgatgaggaa ttgatggcca tggcggagga gacagcgatt ttcggacgtg
    14641 tttcccctca tcaaaagaaa ctcatcatcc aaacgttgaa aaaagcgggt catacaacgg
    14701 ctatgacagg ggacggggtt aatgatatct tggcccttcg tgaggcggat tgttctatcg
    14761 tgatggcgga gggggatcca gcaacccgtc agattgccaa tctggttctc ttaaactcag
    14821 actttaatga tgttcctgag attctcttcg agggtcgtcg cgtggtcaat aacattgccc
    14881 acatcgcccc gattttcttg ataaagacca tctattcctt cctgttagca gtcatctgta
    14941 ttgccagtgc tttactaggt cggtcagagt ggattttgat tttccccttc attccgatcc
    15001 agattaccat gattgaccag tttgtggaag gtttcccacc attcgttttg acttttgagc
    15061 gaaatatcaa acctgtcgag ccaaacttcc tcataagatc catgcttcgt gccctaccaa
    15121 gtgccctcat ggtcgtgttt agcgttcttt ttgtgaaaat ctttggaacg agtcaaggtt
    15181 ggtctgagtt agaaatctca actctcctct attatctctt ggggtcaatt ggtttcttat
    15241 ccgtatttag agcctgcatg ccattcactc tatggcgagt cctcttgatt gtctggtcag
    15301 taggaggctt cctagccaca gctctcttcc caagaatcca aaaactgctt gaaatttcaa
    15361 ccttgacaga acaaacgttg cctgtttatg gtgtcatgat gttggtcttt acagtgattt
    15421 tcatcctgac tagtcgctat caagctagaa aataaagaaa gactgcaatc tgtggattgc
    15481 agttttttta ggtacaagat tgccagccga aatgtggtat aataagaggt aatagagttt
    15541 tggaaagtga gagaagatga tttcaaagag attagaattg gtagcttcct ttgtgtcaca
    15601 gggggctatt ttactagatg tgggaagtga ccatgcttat ctgcctatcg agttggttga
    15661 gagaggccaa atcaaaagcg ctattgcagg tgaggtggtg gaaggtccct atcagtctgc
    15721 ggttaaaaat gttgaggctc acggcctaaa ggagaaaatc caagtccgtt tagccaatgg
    15781 cttggcagct tttgaagaga ctgaccaagt gtctgtcatt accattgctg gcatgggtgg
    15841 tcgtttgatt gctaggattt tagaagaagg tttggggaag ttagctaatg tagaacgttt
    15901 gatcctccag cccaataatc gtgaagacga cttgcgtatc tggctacagg atcatggatt
    15961 ccagattgta gcagaaagca tcttagaaga agctggaaag ttttatgaga ttttggtggt
    16021 ggaagcagga caaatgaagc tatcagccag tgatgttcgc tttggtccct tcttgtccaa
    16081 agaagtcagt ccagtatttg tccaaaaatg gcaaaaagaa gctgagaagc tagagttcgc
    16141 cctcggacaa atcccagaaa aaaatctgga agaacgtcaa gttctagtag ataagattca
    16201 agctatcaag gaggtgctcc atgttagcaa gtgaagtgat ccaagcttat gaagcctttt
    16261 gccctcagga attttctatg gagggagaca gctgtggtct gcaaattggc actttagaca
    16321 agggtatcca aagggtcatg gtggctctcg atattcgtga agagacggtg gctgaagcca
    16381 ttgaaaaggg tgtagacttg attatcgtca agcacgcgcc tatctttcgt cctatcaagg
    16441 acttgcttgc cagccgtccg caaaatcaga tttacatcga cctgattaag catgacatcg
    16501 cagtttatgt cagccatacc aatattgata tcgttgaaaa tgggctcaat gactggtttt
    16561 gtcagatgct aggaatcgag gagacgactt atctgcagga aacaggtcca gaacgtggaa
    16621 ttggacgtat tgggaatatt cagcctcaga cattttggga attggcccaa caggtcaagc
    16681 aagtctttga cctagatagc cttcgaatgg tgcattatca agaggatgat ttgcagaagc
    16741 ctatttcaag agtagcaatt tgtggtggaa gcgggcagtc tttctataag gatgctttgg
    16801 caaagggggc agatgtctat atcactggtg atatctacta ccatactgct caggatatgc
    16861 tgtctgatgg cttgttagca ttggatccag gtcactatat cgaagtgctt tttgtggaaa
    16921 aaatcgcaac actccttact caatggaaag agaaaaaagg ctgggaacta gagattttac
    16981 ctagtcaagc atcgaccaat cctttccgtc atatctagtt agaaagtgaa gacaatgaaa
    17041 aaagttgcca ttattggagc aggaattgtg ggagcaacag ctgcctacta cctctcgaga
    17101 gaaagtgacc tagaggtgac cgtttttgac catgagcaag gtcaagccac caaggccgca
    17161 gcaggaatta tcagtccttg gttttccaaa cgccgtaata aagcctggta caagatggcg
    17221 cgcttggggg ctgattttta tgtggattta ttagctgatt tagagaaatc aggacaagaa
    17281 atcgactttt accagcgttc gggagtcttt ctcttgaaaa aggatgaatc caatttggaa
    17341 gaactttatc aactggccct ccagcgcaga gaagaatctc ccttgatagg gcaattagcc
    17401 attctgaacc aagcctcagc taatgaatta ttccctggtt tgcagggatt tgaccgcctg
    17461 ctctatgctt ctggtggagc gagagtagat ggtcacctct tagtaactcg tttgctggaa
    17521 gccagtcagg tcaagttggt caaagaaaaa gtgactctga cactcttatc atcaggctac
    17581 cagattggcg aagaggtgtt tgatcaggtt attttggcaa cgggagcttg gttgggggac
    17641 atgttagagc ctttaggtta tgaagtggat gtccgtcctc aaaaaggaca actacgagat
    17701 tatcagcttg cccaagacat ggaagattac cctgttgtca tgccagaagg ggagtgggat
    17761 ttgattccct ttgcaggtgg gaaattatcc ttgggcgcta cccatgaaaa tgacatggga
    17821 tttgatttga cggtagatga acccttgctc caacaaatgg aggaggcggc cttgcctcat
    17881 tacccagttt tagctagagt gacttcaagg gctgagcgtg tgggaatccg tgcctatacc
    17941 agtgatttct ctcccttctt tgggcaggtg ccagaattgg caggtgtcta tgcagccagt
    18001 ggactaggtt catcaggcct cacaactggt cctatcattg gttaccatct agcccaactg
    18061 atccaagaca aggagttgac cttggaccct gaaaattacc caattgaaaa ctatgtcaaa
    18121 cgagtaaaaa gcgaataata ctcaatgaaa atcaaagaac aaactaggaa actagccgcg
    18181 ggttactcaa agcactgctt tgaggttgtg gatagaactg acgaagtcag ctcaaaacac
    18241 tgttttgagg ttgtggatag aactgacgaa gtcagtaacc atacccatgg caaggcgacg
    18301 ttgacgtggt ttgaattgga ttttcgaaga gtataagcat tttactgaaa ttttatcagg
    18361 tagtttagga tggcaaatga cattccctgt caaaaatggt aaaataagaa aaaatgatct
    18421 gagaatcgag gaaaaaagat gcaagaaaag attttggtaa ctggtggtgc cggttttatt
    18481 ggaacccaca ctgttattga attgatccaa gcaggccatc aagttgttgt ggtggataat
    18541 cttgtcaata gcaatcgtaa gagtttagaa gttgttgaaa gaatcacagg agttgagatt
    18601 cctttctatg aggcagatat tcgtgacaca gataccctca gagatatttt caagcaggaa
    18661 gaaccgactg gtgtcattca ctttgctggt ttgaaggctg ttggcgaatc aacacgtatc
    18721 cctcttgcct actatgacaa caatatcgct ggaactgtca gccttttgaa agccatggaa
    18781 gaaaacaact gtaaaaacat catcttcagt tcttctgcga cagtttacgg agatccgcac
    18841 acagtgccca tcttggaaga tttcccactt tcagtgacaa acccatacgg tcgtaccaag
    18901 ctcatgctag aggaaatttt gaccgatatt tacaaagcag actcagaatg gaatgttgtc
    18961 ttgcttcgtt actttaaccc aatcggagcc catgagagtg gtgatttggg agaaaatcca
    19021 aacggtattc caaacaatct cttgccatat gtgactcaag tagccgttgg aaaattagag
    19081 caagtgcaag tgtttggaga cgattacgat acggaagatg gaacaggtgt tcgtgactat
    19141 atccacgttg tcgatttggc taagggtcac gttgcagctt tgaaaaaaat ccaaaaaggt
    19201 tcaggactaa acgtttataa ccttggaact ggtaaaggtt actcagttct tgaaattatc
    19261 caaaacatgg aaaaagcggt gggacgtcct attccttacc gcatcgtaga acgtcgccca
    19321 ggtgatatcg ctgcctgcta ctcagaccca gcaaaagcta aagcagaact cggttgggaa
    19381 gcagaactcg acatcaccca aatgtgtgaa gacgcatggc gttggcaaag caagcatcca
    19441 aatggatttg aagactaaga tgatgatttc aatcatcgtc ccttgtttaa acgaagagga
    19501 agtacttcct cttttttatc aggctctgga agctttactt ccagatttgg aaacagaaat
    19561 cgagtatgtc tttgtcgatg atggatcaag tgatgggacc ttggaactct taaaggccta
    19621 tcgggagcaa aatccggcag tccattatat ttctttctct cgaaattttg gcaaagaagc
    19681 agccctttat gcaggcttgc aatatgcgac aggagatttg gtggtggtga tggatgcaga
    19741 cctccaagat cctcctagta tgttgtttga gatgaaaaat gtactagaca aaaatgtaga
    19801 cttggactgc gttgggacac ggagaactag tcgggaggga gaacccttct ttcgcagttt
    19861 ctgtgctgtt ctcttttatc gcctcatgca aaaaatcagc ccagtagctc tgccgtcggg
    19921 tgtccgtgat tttcgtatga tgagaaggtc tgtagtcgat gccattttaa gcttgaccga
    19981 gtccaatcgt ttttcaaagg ggctcttcgc ctgggtgggt ttcaaaacct actatttgga
    20041 ctatccaaat gtcgaaaggc aggctggcaa gacaagttgg agttttaggc aactcttttt
    20101 ctactccatt gaagggatta ttaatttttc agattttccc ttgattatag cctttgtagc
    20161 tggtctccta tcttgttttc tttctctgct gatgaccttt tttgttgtgg ttcggaccct
    20221 cattttgggc aatccgacat ctggttggac ctctctgatg gctgttattc tctttcttgg
    20281 aggcattcaa ctcttgacca ttgggattct cggcaagtat atcagtaaga tttatttaga
    20341 gactaaaaaa agaccacttt atcttatcaa agaaaaaagt gaccttcctg attttacaga
    20401 aaaaaataaa gtgaaaagac tataatttta catggaaatg tgctaaacta gagggagtgg
    20461 attatcgccc ttgatttagg cttcctggtt tccaatcagg gcgcgagttg ggcaattttt
    20521 aacagcctct aagacatcct gactaggaga aatttctttt tccagttggt cagggtcatc
    20581 gtaaaaacgc acgattccat tatcgtggta atcaaataaa tcagaataag tttggcaaag
    20641 cccacaggcg atacatcgtt caggtataag tgtgattttc atgttcatat ttatattgta
    20701 ataagaaaag taaaaaaaaa caaggagtaa ggtatggcaa aagaaccgtg gcaagaagat
    20761 atctatgatc aagaagaatc aagagcagag cgtcggcatc gaaaccacgg aggggctgat
    20821 aggatggcta atcgtatttt gacgatccta gctagtattt tctttgtaat tgtggtggtg
    20881 atggtcatcg ttctcatcta tctatcatcg gggggaagta atcgcacagc agccttaaaa
    20941 gactttcatg attctgatgc aagtgtagta caaatctcat cttcaagcag ttctcatcct
    21001 gagcagagtt cagagccaga atctacttct agtagttcag aagaagctgc taatcctgaa
    21061 ggaacgatta aagttctcgc aggagaaggg gaagcagcta ttgccgctcg tgcaggaatc
    21121 tccattgctc agttagaggc cttgaatcct gggcacatgg ctacaggatc ttggtttgct
    21181 aatccaggtg atgttataaa aataaaatag gagccgatat gaaaacaatt caaattgcta
    21241 ttgatggtcc tgcttccagc ggtaagagta cggtcgcaaa gattattgct aaggattttg
    21301 gattcaccta ccttgataca ggagctatgt atcgtgcagc gacctatatg gctcttaaga
    21361 accaattagg agttgaagaa gtcgaagccc ttctagcctt gttggaccag catccaatca
    21421 gctttggacg ttcagaaact ggagaccagc ttgtttttgt aggagatgtg gatattaccc
    21481 atcctatccg tgaaaatgaa gtgaccaatc atgtttctgc tattgcagca attcctcaag
    21541 tgcgtgagaa actggtttct ctccaacaag aaattgccca gcaaggcggg attgtcatgg
    21601 atggtcgcga tattggaact gttgtattgc cacaagcaga attgaaaatt ttcctagtag
    21661 cttctgttga tgagagagca gagcgtcgtt acaaggaaaa tattgccaag ggaattgaaa
    21721 cagaccttga aaccctaaaa aaggaaattg ctgcgcgtga ctacaaggat agtcatcgtg
    21781 agacttctcc tctcaaacaa gcagaggatg ctgtctacct tgatacaact ggtttgaaca
    21841 ttcaagaagt agttgaaaaa atcaaagcag aagctgaaaa aagaatgtaa atgtagaaaa
    21901 gccgatagat tgatatcggt ttttttctaa atttgtcaaa atgctgattt tagggtataa
    21961 tagttgctgt tcgagaaatt ttgattttca aagaatagtg aatgatgata aggagaaacc
    22021 atgaacaatt taccaaattg tccaaaatgt aactcagagt atgtctacga agacggtgcc
    22081 ctactggttt gcccagagtg tgctcatgag tggaatcctg ctgaagttgc agaagtagaa
    22141 gaaggtcttg tcgctatcga tgccaacgga aataaattga ctgatggtga tacagtaact
    22201 ctcatcaagg acttgaaagt aaaaggtgcg ccaaaagatt tgaaacaagg gacgcgcgtg
    22261 aaaaatatcc gcatcgtaga aggcgatcac aatatcgact gtaagattga tggttttggt
    22321 gccatgaaac ttaaatcaga gtttgtgagg aagatttaag catgtcaaaa cactataaac
    22381 ttgtatttta tagccgtatc ttcttgtttc tagcggcttt tacgggagtt tatcttgaaa
    22441 ttactaggca tggtggtttt gggatgcttc tctattacac ggttctgtcc aacctcttgg
    22501 taactatttt taccctttat cttctaaagg ttatgagccg tgtaggtgaa aattggcaaa
    22561 ggccaagtct cttgcgctta aaaggtgggg tcaccatgag tatcatgatt acctgtgtga
    22621 tttaccattt cctcttggcg cccattgcga ctaatttcta taccctagaa aatttccttt
    22681 gccactatat cgttcccatc tggtttttag cggataccct cttttttgac aaacagggtc
    22741 aatacaagat ttgggatcca gcagtgtgga ccattttacc ctttctgtat atgatgtttg
    22801 ctctttttaa tggcttggtt ctaaaactca atattccaaa tgccaaggat aatcctttcc
    22861 cttacttctt tttgaatgtg aacaagggtt ggaatgttgt gtttaagtgg tgtctgatta
    22921 tctttgttgc ctatatggta gcaggattta ttttctactt tatcaagcaa atcaagagaa
    22981 agtcatccta atactcttcg aaaatctctt caaaccacgt cagtttccat ctgcaacctc
    23041 aaaacactgt tttgaacaac ctgcggctag cttcctagtt tgctctttga ttttcattga
    23101 gtataagata caagggattc attttcgaat ttaagaagga gtctacaaac caatgaggct
    23161 tcttcttttc ttgcttattt gataactatc aaaaaagtag aagattaaga gaaatataga
    23221 aagagatttc atgaaacaat ttttagaacg ggccagcatt ttggctctct ccctcgtttt
    23281 gattacctcc ttttccattt cgagtgccct accagccatg tttgactatt atcagggtta
    23341 ttctaaggaa caaattgagc tcttggtgag cttgccttcc tttggaatca tgatgatgtt
    23401 actgctaaat ggtttcttag aaaaaatatt tcctgagcgc ttacagatta gtttgggctt
    23461 gctgatttta tcattgagcg gtacagctcc cttctggtac caagcctatc cctttgtctt
    23521 tggaacacgg cttctctttg gtttgggtct tgggatgatc aatgccaagg ccatttctat
    23581 tatcagtgaa cgctaccaag gaaaaaggcg aattcagatg ttagggctac gcgcttctgc
    23641 agaggtcgtt ggagcttctc tcattacctt ggccgtcggt cagttgttgg cctttggttg
    23701 gacagctatc tttctagcct atagtgctgg atttttggtg ctgccccttt atctgctctt
    23761 tgtcccttat ggaaaatcaa agaaagaagt caagaaaaga gcgaaggaag caagtcgttt
    23821 aactcgagaa atgaaaggct tgatttttac cttagctatc gaagcggcag ttgtagtttg
    23881 taccaataca gctattacca tccgtattcc aagtttgatg gtggaaagag gattggggga
    23941 tgcccagtta tctagttttg ttcttagtat catgcagttg atcgggattg tggctggggt
    24001 gagtttttct ttcttgattt ctatctttaa agagaaactg ctcctctggt ctggtattac
    24061 ctttggcttg gggcaaatcg tgattgcctt gtcttcatcc ttgtgggtgg tagtagcagg
    24121 aagtgttctg gctggatttg cctatagtgt agtcttgacg acggtctttc aacttgtctc
    24181 tgaacgaatt ccagctaaac tcctcaatca agcaacttca tttgctgtat taggctgtag
    24241 tttcggagcc tttacgaccc cattcgttct aggtgcaatt ggcttactaa ctcacaatgg
    24301 gatgttggtc tttagtatct taggaggttg gttgattgta atctctatct ttgtcatgta
    24361 cctacttcag aagagagctt aggattgatt cctaagtttt tcttttgata ctcaatgaaa
    24421 atcaaagagc aaactatagt tgattgagtt tggaatagta cgctgtagct gctaaaatat
    24481 ttctagaaat taatttgact ttcctaatag agttgttcat atcttatttc aatctactat
    24541 agaaagctag ccgcaggttg ctcaaaatag tgtgttgagg ttgcagatgg aaactgacgt
    24601 ggtttgaaga gattttcgaa gagtatgaga tttttatacc catacaatat ttgttttagg
    24661 tccttgttta cagactaatt tcttgataaa atagaggcta tgacgagata taaagcaact
    24721 atttcctatg atggttatgc ctttgctggc tttcagcgcc agcctcatgc gcgtagcgtt
    24781 caggaagaaa ttgaaaaaac cttgaccaga ttaaataaag ggcaagccat tactgttcac
    24841 ggtgctggta ggacagatag tggggttcat gccctgggac aggtgattca ttttgacctg
    24901 ccttatcaga tggatgagga gaaactccgt tttgccttgg atacccagtc tcctgaagat
    24961 attgatgtga tttcgattga gcttgtggca gatgattttc attgccgtta tgccaagcat
    25021 agcaagacct atgagtttac tgtggataga ggacgtccca aaaatccgat gcgccgtcac
    25081 tatgccaccc actttcccta cccactcgat gtggaacgaa tgcagattgc aatcaaaaag
    25141 ctagagggaa cccatgattt taccggtttt acagcctctg ggactagtgt agaggataag
    25201 gttcgcacca tcacagaagc tagtttaata gtcgatgaga caggacaatt tttgaccttt
    25261 accttttcag gaaatggttt cttgtataaa cagattcgca atatggtggg gactcttctc
    25321 aaaatcggta acaaccgcat gccagtagag cagattgatc tcatcttgga gaagaaggac
    25381 aggcaacttg caggtcccac tgcagcacca aatggtttgt atttaaagga gattcgttat
    25441 gaagaataat cgtattttag cactttctgg aaatgatatt tttagtggtg gtggactgtc
    25501 agctgatttg gctacctata ccttgaacgg cttgcatggg tttgtagcag tgacttgttt
    25561 gacagccttg acagaaaaag gatttgaagt ctttccaact gatgatacca tttttcaaca
    25621 tgaattagat agcttgcgtg atgtggaatt tgggggaatt aagattggtc ttctccctac
    25681 tgtcagcgtg gctgagaagg ccttggactt tatcaaacaa cgcccaggag tacctgtggt
    25741 gttggatcct gtcttggtct gcaaggaaac gcatgatgta gctgtcagtg agctctgcca
    25801 agagttgatt cgctttttcc cttatgtcag tgtgattacg cctaatctcc cagaagcaga
    25861 attattatcc ggtcaggaaa ttaaaacctt ggaagacatg aaaactgcag cgcagaaatt
    25921 gcatgattta ggagcgccag cagtcattat caagggaggc aatcgtctta gtcaggacaa
    25981 ggctgtggat gtcttttatg atggacagac ctttactatc ctagaaaatc cagttatcca
    26041 aggccaaaat gctggtgcag gttgtacctt tgcctctagc attgccagtc acctggttaa
    26101 aggtgataaa cttttgccag cagtagaaag ctctaaggct ttcgtttatc gtgctattgc
    26161 acaagcagat cagtatggag taagacaata tgaagcaaac aaaaacaact aaaatcgccc
    26221 ttgtatccct attaaccgcc ctttctgtgg ttctaggtta tttcttaaaa atcccaacac
    26281 ctacaggaat tctaactctt ttagatgctg gtgtcttctt tgcggccttt tactttggta
    26341 gtcgtgaagg agcggtagtc ggaggactag caagtttctt gattgacctc ttatcaggct
    26401 accctcagtg gatgttcttt agcttggtca accatggctt gcagggattt ttcgcaggat
    26461 ttaaaggaaa aagtcagtgg ttaggcctta ttttagcaac tattgccatg gtaggaggct
    26521 acgccttggg ttctgctttg atgaatggct gggcagcagc cctcccagaa attctaccga
    26581 attttatgca aaatatggta gggatgattg taggatttat tcttagtcaa agtatcaaga
    26641 agattaagta atactcttcg aaaatctctt caaaccacgt cagcttccat ctgcaacctc
    26701 aaaacactgt tttgagcagc ctgaggctag ctttctagtt tgcactttga ttttcattga
    26761 gtataaagag gttgagtaaa aagtatttca aataagaaaa acgcatagta tcaggtgttg
    26821 aatgaactgc cccccaaaag ttagacagaa aaaatctaac ttttggggtg tttttattat
    26881 gaaactaact tatgatgata aagttcagat ctatgaactt agaaaacaag gatatagctt
    26941 agagaagctt tcaaataaat ttgggataaa caattctaat attaggtaca tgattaaatt
    27001 gattgatcgt tacggaatag agttcgtcaa aaaaggaaaa aatcgttact attctcctga
    27061 tttaaaacaa gaaatgatt
//
LOCUS       DAVHEX010000027        25569 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVHEX010000027 DAVHEX010000000
VERSION     DAVHEX010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25569
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.027"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..2385
                     /locus_tag="VVL45_001112"
     CDS             <1..2385
                     /locus_tag="VVL45_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HEU4187297.1"
                     /translation="NKEHHLNTKEVLSVTAMNNNEFITNLDEANKIIVHYADGTKDYF
                     NLSSSSEGLSNVKEYTITDLGIKYTPNIVQKDNTTLVNDIKSILESVELQSQTMYQHL
                     NRLGDYRVNAIKDLYLEESFTDVKENLTNLITKLVQNEEHQLNDSPAARQMIRDKVEK
                     NKAALLLGLTYLNRYYGVKFGDVNIKELMLFKPDFYGEKVSVLDRLIEIGSKENNIKG
                     SRTFDAFGQVLAKYTKSGNLDAFLNYNRQLFTNIDNMNDWFIDATEDHVYIAERASEV
                     EEIKNSKHRAFDNLKRSHLRNTILPLLNIDKAHLYLISNYNAIAFGSAERLGKKSLED
                     IKDIVNKAADGYRNYYDFWYRLASDNVKQRLLRDAVIPIWEGYNAPGGWVEKYGRYNT
                     DKVYTPLREFFGPMDKYYNYNGTGAYAAIYPNSDDIRTDVKYVHLEMVGEYGISVYTH
                     ETTHVNDRAIYLGGFGHREGTDAEAYAQGMLQTPVTGSGFDEFGSLGINMVFKRKNDG
                     NQWYITDPKTLKTREDINRYMKGYNDTLTLLDEIEAESVISQQNKDLNSAWFKKIDRE
                     YRDNNKLNQWDKIRNLSQEEKNELNIQSVNDLVDQQLMTNRNPGNGIYKPEAISYNDQ
                     SPYVGVRMMTGIYGGNTSKGAPGAVSFKHNAFRLWGYYGYENGFLGYASNKYKQQSKT
                     DGESVLSDEYIIKKISNNTFNTIEEFKKAYFKEVKDKATKGLTTFEVNGSSVSSYDDL
                     LTLFKEAVKKDAETLKQEANGNKTVSMNNTVKLKEAVYKKLLQQTNSFKTSIFK"
     gene            2446..4167
                     /gene="pabB"
                     /locus_tag="VVL45_001113"
     CDS             2446..4167
                     /gene="pabB"
                     /locus_tag="VVL45_001113"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEU4187298.1"
                     /translation="MHRKTVIDFRTLGERYTFTQPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDIVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLNANPFAIYNRMVVEQEA
                     GYNAYVEHDEMTVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFRSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGKISQKQLMFEE
                     QHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANRDYRLRISLSKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRPHLSLGEQEKIYHNKSGELLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQHLLETGQVEEKVLTLADLTQAEAIYGCNAVRGLY
                     ELSLEEN"
     gene            4168..4785
                     /locus_tag="VVL45_001114"
     CDS             4168..4785
                     /locus_tag="VVL45_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU4187299.1"
                     /translation="MKLIFLHGLGQSAESWKEVRNLLTDYPSEAIELFPSGVSNYQQA
                     KERVYQHLAQETEPFVLIGLSLGAALTLELSSYDLPNLRALILSGCPLKLAGNILFYL
                     QLLIFKLLPKRVFEKQGADKTLMVGVSEELKTLDLTDIAGTYPYPTLLICGSKDKPNL
                     SSMKALHRLLTDSQFQIIPDGPHVLNKAKPKEFVEKTRSFLELLK"
     gene            4835..5833
                     /locus_tag="VVL45_001115"
     CDS             4835..5833
                     /locus_tag="VVL45_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU4187300.1"
                     /translation="MNKRLFSKMSLVTLPILALFSQSVLAEENIHFSSCKEAWANGYS
                     DIHEGEPGYSAKLDRDHDGVACELKNAPKGAFKAKQSTAIQINTSSATTSGWVKQDGA
                     WYYFDGNGNLVKNAWQGSYYLKADGKMAQSEWIYDSSYQAWYYLKSDGSYAKNAWQGA
                     YYLKSNGKMAQGEWVYDSSYQAWYYLKSDGSYARNAWQGNYYLKSDGKMAKGEWVYDA
                     TYQAWYYLTSDGSYAYSTWQGNYYLKSDGKMAVNEWVDGGRYYVGADGVWKEVQASTA
                     SSSNDSNSEYSAALGKAKSYNSLFHMSKKRMYRQLTSDFDKFSNDAAQYAIDHLDD"
     gene            5960..6919
                     /locus_tag="VVL45_001116"
     CDS             5960..6919
                     /locus_tag="VVL45_001116"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEU4187301.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPISCTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            7016..7855
                     /locus_tag="VVL45_001117"
     CDS             7016..7855
                     /locus_tag="VVL45_001117"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEU4187302.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(7903..8073)
                     /locus_tag="VVL45_001118"
     CDS             complement(7903..8073)
                     /locus_tag="VVL45_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEU4187303.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            8194..9078
                     /gene="miaA"
                     /locus_tag="VVL45_001119"
     CDS             8194..9078
                     /gene="miaA"
                     /locus_tag="VVL45_001119"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEU4187304.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEGIHNRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVDQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQETLYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTLEEASESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            9071..10309
                     /gene="hflX"
                     /locus_tag="VVL45_001120"
     CDS             9071..10309
                     /gene="hflX"
                     /locus_tag="VVL45_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEU4187305.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            10302..10925
                     /locus_tag="VVL45_001121"
     CDS             10302..10925
                     /locus_tag="VVL45_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEU4187306.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTDQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGLGRIFFENEEKISDDLLFEIAQIFPHQLVFEESGKIYMKPVEDEEVISLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            10940..11869
                     /locus_tag="VVL45_001122"
     CDS             10940..11869
                     /locus_tag="VVL45_001122"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEU4187307.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTGASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            11890..12645
                     /locus_tag="VVL45_001123"
     CDS             11890..12645
                     /locus_tag="VVL45_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU4187308.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEPDAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(12699..13607)
                     /locus_tag="VVL45_001124"
     CDS             complement(12699..13607)
                     /locus_tag="VVL45_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU4187309.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            13671..13940
                     /locus_tag="VVL45_001125"
     CDS             13671..13940
                     /locus_tag="VVL45_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEU4187310.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            13940..14320
                     /locus_tag="VVL45_001126"
     CDS             13940..14320
                     /locus_tag="VVL45_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU4187311.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            14409..14636
                     /locus_tag="VVL45_001127"
     CDS             14409..14636
                     /locus_tag="VVL45_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187312.1"
                     /translation="MKEKKKNPLFVGILSIILGLLFPIVGLILGIIGLVLAISYQKES
                     QLDYKIEKILNILGIVISVVNWIVAIALIFR"
     gene            complement(14676..15401)
                     /locus_tag="VVL45_001128"
     CDS             complement(14676..15401)
                     /locus_tag="VVL45_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HEU4187313.1"
                     /translation="MRLDNLLAQEKISRKAMKQALLRGEILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGAVTANKDKELPTVMDLLPSNIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQTFQKGIVFLDDTVC
                     KPAKLEILSASPSLSQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLA
                     EGNYRPLNQKELQIIKNYLEMSR"
     gene            15563..17404
                     /gene="typA"
                     /locus_tag="VVL45_001129"
     CDS             15563..17404
                     /gene="typA"
                     /locus_tag="VVL45_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEU4187314.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGIKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            17424..17678
                     /locus_tag="VVL45_001130"
     CDS             17424..17678
                     /locus_tag="VVL45_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEU4187315.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
     gene            18042..18341
                     /locus_tag="VVL45_001131"
                     /pseudo
     CDS             18042..18341
                     /locus_tag="VVL45_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
     gene            18412..20433
                     /locus_tag="VVL45_001132"
     CDS             18412..20433
                     /locus_tag="VVL45_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU4187316.1"
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKD
                     TQEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFTYATYGQGTLPKEFQEASQ
                     ESRERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILI
                     SLAIFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLLSALLSFL
                     GGGVVLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLSYKALVPVIKGR
                     LPLKRLMALTLLCQLVAVFTVGYAVKTGLTSYQRLKELEISKQAWQDRADYYQISFGL
                     GDRGKDTENQSKWYAFAKEAIEEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANT
                     LYVSPSYLDKEKVVVDAETKQKLAHLQKGEFILLLPEHLRSREAELKKVFEERLSYYG
                     KSGEEASAPLDYEMKAHVSYLSMGEKRFVYNNGENPVSTQYLTDPILVVFTPTSTGDS
                     FISLSSWSINAGKQLFIKGYESGLELLKKAGIYEQVSYLKEGRSVYLTRYNEVQTETA
                     TLILGAIVGIASSLLLFYSVNLLYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFV
                     FGASLFILSSRDLVIGLLTLLVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            20435..21076
                     /locus_tag="VVL45_001133"
     CDS             20435..21076
                     /locus_tag="VVL45_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4187317.1"
                     /translation="MIELKNISKKFGSRQLFSDMNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNRVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            21180..22532
                     /locus_tag="VVL45_001134"
     CDS             21180..22532
                     /locus_tag="VVL45_001134"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEU4187318.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQSLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLAKDLTSKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRDVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            22534..23592
                     /locus_tag="VVL45_001135"
     CDS             22534..23592
                     /locus_tag="VVL45_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEU4187319.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKCGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLAKVEHVGA
                     VTKVSDKNTPEPDELVDIQTHFNPKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELRQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            23602..24801
                     /gene="divIB"
                     /locus_tag="VVL45_001136"
     CDS             23602..24801
                     /gene="divIB"
                     /locus_tag="VVL45_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HEU4187320.1"
                     /translation="MSKDKKNEDKETLEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEADKEKEEKEEPES
                     KEKEEQDKKLAKKATKEKPAKAKIPGIHILRAFTILFPSLLLLFVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVVDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKKQEEESNRNQT
                     TQRSSRR"
BASE COUNT         7851 a         4629 c         5612 g         7477 t
ORIGIN      
        1 aataaagaac accatctaaa tactaaagaa gttctttctg ttaccgcgat gaacaacaat
       61 gagtttatca caaacctaga tgaagctaat aaaattattg ttcactatgc ggacggtaca
      121 aaagattact ttaacttgtc ttctagcagt gaaggtttaa gtaatgtaaa agaatatact
      181 ataactgact taggaattaa atatacacct aatatcgttc aaaaagataa cactactctt
      241 gttaatgata taaaatctat tttagaatca gtagagcttc agtctcaaac gatgtatcag
      301 catctaaatc gattaggtga ctatagagtt aatgcaatca aagatttata tttagaagaa
      361 agcttcacag atgttaaaga aaacttaaca aacctaatca caaaattagt tcaaaacgaa
      421 gaacatcaac taaatgattc tccagctgct cgtcaaatga ttcgtgataa agtcgagaaa
      481 aacaaagcag ctttattact aggtttaact tacctaaatc gttactatgg agttaaattt
      541 ggtgatgtta atattaaaga attaatgcta ttcaaaccag atttctatgg tgaaaaagtt
      601 agcgtattag acagattaat tgaaatcggt tctaaagaga acaacattaa aggttcacgt
      661 acattcgacg cattcggtca agtattggct aaatatacta aatcaggtaa tttagatgca
      721 tttttaaatt ataatagaca attgttcaca aatatagaca atatgaacga ttggtttatt
      781 gatgctacag aagaccatgt ctacatcgca gaacgcgctt ctgaggtcga agaaattaaa
      841 aattctaaac atcgtgcatt cgataattta aaacgaagtc accttagaaa tactatactc
      901 ccactactga atattgataa agcacatctt tatttaattt caaattataa tgcaattgcc
      961 tttggtagtg cagagcgatt aggtaaaaaa tcattagaag atattaaaga tatcgttaac
     1021 aaagctgcag atggttatag aaactattat gatttctggt atcgtctagc gtctgataac
     1081 gttaaacaac gactactaag agatgctgtt attcctattt gggaaggtta taacgctcct
     1141 ggtggatggg ttgaaaaata tggccgctat aataccgaca aagtatatac tcctcttaga
     1201 gaattctttg gtcctatgga taagtattat aattataatg gaacaggagc ttatgctgct
     1261 atatatccta actctgatga tattagaact gatgtaaaat atgttcattt agaaatggtt
     1321 ggtgaatacg gtatttcagt ttacacacat gaaacaacac acgtcaacga ccgtgcgatt
     1381 tacttaggtg gctttggaca ccgtgaaggt actgatgctg aagcatatgc tcagggtatg
     1441 ctacaaactc ctgttactgg tagtggattt gatgagtttg gttctttagg tattaatatg
     1501 gtatttaaac gcaaaaatga tgggaatcag tggtatatta cagatccaaa aactctaaaa
     1561 acacgagaag atattaatag atatatgaag ggttataatg acactttaac tcttcttgat
     1621 gaaattgagg ctgaatctgt gatttctcaa caaaataaag atttaaatag tgcatggttc
     1681 aaaaaaatag atagagaata ccgtgataac aataaattaa atcaatggga taaaattcga
     1741 aatctaagtc aagaagagaa aaatgaatta aatattcaat ctgttaatga tttagttgat
     1801 caacaattaa tgactaatcg caatccaggt aatggtatct ataaacccga agcaattagc
     1861 tataacgatc aatcacctta tgtaggtgtt agaatgatga ccggtatcta cggaggtaat
     1921 actagtaaag gtgctcctgg agctgtttca ttcaaacata atgcttttag attatggggt
     1981 tactacggat acgaaaatgg gttcttaggt tatgcttcaa ataaatataa acaacaatct
     2041 aaaacagatg gtgagtctgt tctaagtgat gaatatatta tcaagaaaat atctaacaat
     2101 acatttaata ctattgaaga atttaaaaaa gcttacttca aagaagttaa agataaagca
     2161 acgaaaggat taacaacatt cgaagtaaat ggttcttccg tttcatcata cgatgattta
     2221 ctgacattgt ttaaagaagc tgttaaaaaa gatgccgaaa ctcttaaaca agaagcaaac
     2281 ggtaataaaa cagtatctat gaataataca gttaaattaa aagaagctgt ttataagaaa
     2341 cttcttcaac aaacaaatag ctttaaaact tcaatcttta aataattgaa atctctcatc
     2401 tgctttgcgg atgagggttt tcttatttta tgctatactt aaggtatgca tagaaaaaca
     2461 gtgattgatt ttaggacttt gggggagaga tataccttta cccagcctat taaagagttg
     2521 aaaacgagaa atgtagaaga agtggcagat ttgctggcac aagtggaaag ctaccaagag
     2581 caaggttatt atgtggtggg gtatgtcagc tacgaggctg cacctgcttt tgaggaaaaa
     2641 ttagcagttc acaaggatcc cctactggga gagtacctgc tttactttac tgttcatgat
     2701 atcgtagaaa cttcccctat tcctctgact tatgaggata ttgatttgcc ctcaaattgg
     2761 caggaagtaa cgtctgcagc agactatgaa aaggccattg cccaaattca ccatcattta
     2821 cggcagggag atacctatca ggtcaactac accgtccaac ttaagcaaaa gttaaatgcc
     2881 aatccttttg ccatctacaa tcgtatggtg gtagagcagg aggcgggcta caatgcctat
     2941 gtggaacatg acgagatgac agtgatttcc atgagcccag agctcttttt tgagcaaaat
     3001 gatcgcgagt tgacaacacg accaatgaag gggacgactc agcgtggggt aactgaccaa
     3061 gaagatcttg aacaggccag ttggttggaa caggatccca aaaatcgctc tgaaaatatg
     3121 atgattgtgg acctcttgcg caatgatatg aaccgtattt ctgaagttgg gagcgagcac
     3181 gtggagcgtc tgtgtcaggt agagcagtat tcaactgttt ggcagatgac ttcgaccatc
     3241 aagagtcagt tgcgagagga tgtggacctt gttgaaatct tccgctcact ctttccttgt
     3301 ggttccataa cgggtgcacc gaaaattgcg acaatggaga ttatcaagga cttggagcct
     3361 caaccgcgtg gagtctactg tggaacgatt ggtctcttgc ttccaaatgg acgacggatt
     3421 tttaatgtgg ccattcgtac cattcaactt cacaaaggtc aagccattta tggagttggc
     3481 ggagggatta cttgggatag cacatgggaa tctgaatacc gagaggttca tcaaaaggca
     3541 gcagttctct atcgtaaaca agctcgcttc caattaatta caactggaaa aatcagccaa
     3601 aaacaactga tgtttgaaga acaacatctg gaaaggctga gaaaagctag tcgttatttt
     3661 gcttttcctt ttgatgcaga agacttggga cacaagattg aggaagagtg tcaggattgt
     3721 gaagctaatc gagattaccg cttgcgaatc agccttagca aatctggaga gatagaagtc
     3781 aatcgtcaag tattaacccc tctcagtaca agcttttgtc aggcccaagt ctgtcttcag
     3841 gaagctgctt tgaatcaatc ctttacctac tttaaaacca ctcaccgacc gcatttgagc
     3901 ctaggagaac aagagaagat ttaccacaat aagtcaggag aactgcttga aacctctata
     3961 ggaaatttgg ttctgaaaat cgctggaaaa ctctacacac cgcctatccg acttggaatc
     4021 ttgccaggaa tttaccgtca gcatttgcta gaaacaggac aggtagaaga gaaagtcttg
     4081 accttggcag atttaaccca agcagaagct atttacggct gtaatgcagt gagaggcttg
     4141 tatgaattaa gccttgagga gaactagatg aaattaatat ttttgcatgg gttagggcag
     4201 tcagcagagt cttggaaaga agttcggaat ctactgacag attatccttc tgaggctatc
     4261 gagttatttc cttctggagt tagtaactat caacaagcta aagagcgagt ttatcagcac
     4321 ctagctcaag agacagaacc ttttgttttg attggattgt ccttaggtgc tgccctgaca
     4381 ctagaacttt ccagttacga tttaccaaat cttcgagctt tgattctgtc aggatgcccg
     4441 cttaaattag cagggaatat cctattttat cttcaattgc tgatttttaa actacttccc
     4501 aaaagagtat ttgaaaaaca gggagcagac aagactctta tggtcggagt ttctgaggag
     4561 ttgaagacac ttgatttaac ggatatagca ggaacttacc cttatccaac cttactaatt
     4621 tgtggcagca aagataaacc gaatcttagt tctatgaaag ctctccatag actgttaaca
     4681 gattcgcagt ttcagatcat tcctgatggc cctcatgtct tgaataaggc aaaaccaaag
     4741 gagtttgtag aaaagaccag aagtttcctt gaattgctga aataagtttg ataaaataat
     4801 attgaaatcg atatcataat tatgggagaa agaaatgaat aaacgtctat tttcaaaaat
     4861 gagtctggtg acgttgccaa ttttagcctt gttttcacaa tcagttttgg cggaagaaaa
     4921 catccatttt tcgagctgta aggaagcttg ggcgaatggc tattcggata ttcacgaggg
     4981 agaacctggt tattctgcca agttagaccg tgatcatgat ggtgtggctt gcgaattgaa
     5041 aaatgctcct aagggtgctt ttaaagcaaa acagtcaacg gctattcaaa tcaacacaag
     5101 ttcagcaaca acaagtggtt gggttaagca ggacggcgct tggtactact ttgatggaaa
     5161 tggaaatcta gtgaaaaatg catggcaggg aagctattac ctgaaagctg atggtaaaat
     5221 ggcacagagt gaatggattt atgactcttc ttatcaagct tggtattatt tgaaatcaga
     5281 tggttcttat gcaaaaaatg catggcaagg agcttattac cttaaatcaa acggtaaaat
     5341 ggcacaaggt gagtgggttt atgattcttc ttaccaagca tggtattact tgaaatcaga
     5401 tggttcatat gctcgcaatg catggcaagg aaactactat ttgaaatcag atggtaaaat
     5461 ggctaaaggt gaatgggttt atgatgccac ctatcaagct tggtattatt tgacatcaga
     5521 tggttcttat gcttacagta catggcaagg aaattactat ctaaaatcgg atggtaaaat
     5581 ggctgtcaat gaatgggttg atggtggacg ttattatgtt ggcgctgacg gagtttggaa
     5641 ggaagttcaa gcaagtacag cttcttctag taatgatagc aatagtgaat attctgctgc
     5701 tttaggaaag gcaaaaagtt ataattcgtt attccacatg tcaaaaaaac gtatgtatag
     5761 acaattaact tctgattttg ataaattttc aaatgatgca gctcaatatg ccattgatca
     5821 tttagatgat taacagatac tggtaggcga aaaaattcct tataattcat tcacgcaaac
     5881 ctttgcataa gtgaatggat tttgatatac tagtactgta agcattttca attactacac
     5941 aatgaaaaag gagaatatga tgagtcaaaa gattattggg attgaccttg gtggaacttc
     6001 tatcaaattt gcaatcttaa caacagcagg agaaatccaa ggaaaatggt caatcaagac
     6061 caacattttg gatgagggaa gtcatatcgt tgatgatatg attgagtcta ttcaacatcg
     6121 tttggacttg cttggattgg cagcagcgga cttccaaggc attggaatgg gatcaccagg
     6181 tgtggttgac cgtgacaaag ggactgttat cggtgcctac aacttgaact ggaaaaccct
     6241 tcaaccaatc aaacaaaaga ttgaaaaagc tttgggcatt ccatttttca tcgataatga
     6301 tgccaacgta gcagctcttg gtgagcgctg gatgggtgct ggagataacc aaccagacgt
     6361 tgtctttatg acactcggta ctggtgttgg tggtggtatt gtcgcagaag gcaaattgct
     6421 tcatggtgtt gctggtgcag caggtgaact tggtcatatc actgttgact ttgaccaacc
     6481 aatctcatgt acttgtggta aaaaaggctg ccttgagaca gttgcttcag caacagggat
     6541 tgtcaacttg actcgtcgct atgccgatga atacgaaggc gatgcagcct tgaaacgctt
     6601 gattgataac ggagaagaag taactgctaa gactgtcttt gatctcgcaa aagaaggaga
     6661 cgaccttgct ttgattgttt accgtaactt ctcacgttac ttgggaatcg cttgtgctaa
     6721 cattggttca atcctaaacc catcaacaat cgtcatcggt ggtggtgtgt cagctgcggg
     6781 agaattcctt ctacaaggtg ttcaaaaagt ttacgatgaa aatagtttcc cacaagtacg
     6841 cacatctact aaattagctc ttgcaactct aggaaatgac gctggagtta tcggagcagc
     6901 atcacttgta ttgcaataag ataatgagag aggaaaatgc taacctagag ttagtaatgt
     6961 tcctctttta ttttttatgc tatactagtt aggacaatga atgaggtgaa acgaaatgac
     7021 aaaagcagat acgattttta aagagaatat tgaacgcatt ctaaaggaag gtgttttctc
     7081 agagcaggct cgtcctaagt acaaggatgg gactgttgct aattccaagt atgtaactgg
     7141 tgccttttcc gagtacgact tatctaaagg agaatttcct atcacaacct tgcgtcccat
     7201 tgcaatcaaa tccgccatca aagaagttct ctggatttac caagaccagt caaatagctt
     7261 agaagtgctt aatgacaagt acaatgttca ctactggaat gactgggaag ttggagacac
     7321 gggaaccatt ggtgagcgct atggtgccgt tgttaagaaa cacgacatta tcaataagct
     7381 tctcaaacag ttggaaacca atccttggaa ccgccgcaat attatttcgc tctgggatta
     7441 ccaagctttc gaagaaacag atgggctgct cccgtgcgcc tttcagacca tgtttgatgt
     7501 ccggcgtgtt gatggggaaa tctatctgga tgcgaccttg acccagcgct ccaatgatat
     7561 gctggtggcc caccacatca acgctatgca gtatgtggct ttgcagatga tgattgccaa
     7621 acattttggc tggaaggttg ggaagttctt ctacttcatc aacaacctcc atatctatga
     7681 taatcaattt gaacaagctc aggaattgct ccgtcgggag ccgtcaaact gccaaccacg
     7741 cttggtttta aatgttcctg atgggactaa tttctttgat atcaaagcag aagattttga
     7801 gttggtggat tatgaccctg ttaagccaca gttgaagttt gacctagcta tttaaaagaa
     7861 tagaaaaaag aagttgagaa taatcccaac ttcttttgtt tcttaacgtg atacgcggcg
     7921 acgagctgct tttttacggt tttcttcgat gaaagctgct ttttgctctt ctggttcgat
     7981 tactttcttt ttaaatgcgt atactgcacc tgcaacggca gcgacagttc ctgcgacacc
     8041 tgttacaaga cctttagcga atcctttagc catgagtctt cctcctttat attctcaatc
     8101 agccagcctc ctcaagaggt cacatttttc tgactgacct ttttgtgtta taataatagt
     8161 aacgaaaaaa tgggaatttt tcaaggaaaa aagatgaaaa caaaaataat tgtgattgtt
     8221 ggaccgactg ctgttggaaa gacggctcta gctattgaag ttgcaaagcg ttttaatggc
     8281 gaagtggtta gtggagatag ccagcaagtc tatcgaggac ttgatattgg gacggccaag
     8341 gctagtccag aagagcaggc agctgttcct catcatttaa tcgatgttag agagataacc
     8401 gagtcttact cggcttttga ttttgtttca gaagctaaga tgactattga gggtattcac
     8461 aaccgtggca agctagccat tatcgccggt gggactggac tttatatcca gagcttgcta
     8521 gaaggttacc acctaggtgg ggagactcct catgaggaga ttttagctta tcgagctagt
     8581 ttggagccat attcagatga ggaattagcc catttggtgg atcaagcagg ccttgagatt
     8641 ccccagttta atcgtcgtcg tgctatgcgt gccttagaaa ttgcccattt tggtcaggat
     8701 ttggaaaatc aagagacctt gtatgaaccg ctgattatct gcttggatga tgaacgtagt
     8761 caactttatg agcgtatcaa ccaccgagtg gatttgatgt ttgaggctgg gcttttggat
     8821 gaggccaagt ggctgtttga ccattcccct aatgtacagg ctgctaaagg aattggctat
     8881 aaggaactct ttccttattt ccgtggagag cagaccttgg aggaagctag tgagagtctt
     8941 aaacaggcga ctcgtcgttt tgccaagcgt cagctgacct ggttccgtaa tcgcatgcag
     9001 gtcacctttt atcagattgg agaatctggt gtacaagacc gcattttaag ccagatagag
     9061 gagtttttag atgattgaaa cggagaaaaa agaggagcga gtcctgctga ttggtgtgga
     9121 attgcagggt atggacagtt ttgacctctc catggaagaa ttggctagtt tagcgaaaac
     9181 ggcaggggca gtcgttgtag atagctacag acaaaaacgt gaaaaatatg attccaagac
     9241 cttcgtcggc tctggtaagt tggaagagat tgcgcttatg gtggatgcag aagaaatcac
     9301 tactgtcatc gtcaacaatc gtctgacccc aaggcagaat gtcaatctag aggaagttct
     9361 cggtgttaag gtcattgacc gtatgcagtt gattttggat atctttgcca tgcgggctcg
     9421 aagccatgaa gggaagctcc aagtccacct agcccaactc aaataccttt tgcctcgctt
     9481 ggttggtcag gggattatgc tcagccgtca ggcaggggga attggttccc gtggtcctgg
     9541 tgaaagccag ctggagctga accgtcgtag cgttcgcaat caaatcacgg atatcgagcg
     9601 ccagctcaag gtggttgaga aaaatcgtgc gactgtcaga gaaaaacgtt tggagtctag
     9661 cacttttaag attggtttga ttggttatac taatgctggg aaatcaacta tcatgaacat
     9721 cttgaccagt aagacccagt atgaagcaga tgagctcttt gcgactctgg atgcgacaac
     9781 caagagtatt catctgggag gcaatctcca agtaactttg acagataccg ttggctttat
     9841 ccaagatttg ccgacagagt tggtgtccag tttcaagtca accttggaag aaagcaagca
     9901 tgtggacctt ctggttcatg ttatcgatgc tagcaatcct taccacgagg agcatgaaaa
     9961 aacggttctc tccatcatga aagacctgga catggaagat attcctcact tgacgcttta
    10021 taataaagcg gatttggtgg aggatttcac gcctacccaa acgccatata ccctcatttc
    10081 tgccaagtct gaggacagtc gtgaaaactt gcaagcatta ttgctagata agattaagga
    10141 aatttttgaa gcatttaccc tgcgagtgcc tttttcaaag tcctacaaga ttcatgattt
    10201 agagagtgtt gcaattctgg aagaacgtga ttatcaggaa gacggcgaag tgattacagg
    10261 ctacatttcc gagaaaaata aatggaggtt agaagaattt tatgactgat attaaaactt
    10321 tggctctaaa gtatggaggt tatacaagtc tggacaaggt ctatctggat cagcttctag
    10381 ctggtaaaac agatcaggag cagttaacac ttattacacc tccgccaagt gttgttaatg
    10441 cctactttgc agaactctat cagaaaaaga gtcctcaggc tgcgacggat tattttgcag
    10501 aactcagtca ggaactgaat ctttacaata ctgagccaag tttcacccta gaaaataagc
    10561 cttttattcg gcttaatctg tctggtaaat cctttggttt ttgttatgag agtgacggcc
    10621 ttggtcggat tttctttgaa aatgaggaga aaatctcgga tgacttgctc tttgaaattg
    10681 cgcaaatttt cccccatcaa ttagtctttg aagagtctgg taagatttac atgaagcctg
    10741 tcgaggacga ggaagttatc agcctagaaa gtctcacagc tttgactgat ttggaaagat
    10801 tggctgatgg tcgaaaacgt ctcaaaggct acagccaaga ggatttattg caagaagctg
    10861 ctgctttttc tggcaaacgc tatttccgat cggaaaaccg cacagccatg ttatatattg
    10921 attaattaga aagtatcgaa tggatattca atttttagga acgggggctg gtcagccctc
    10981 taaagcccgc aacgtttcaa gtctcgccct gaaacttttg gatgagatta acgaagtttg
    11041 gctctttgac tgtggagaag gtacgcaaaa tcgcattctg gaaaccacaa ttcgaccacg
    11101 taaggtcagc aaaatcttta ttacccatct gcatggagac cacatttttg gtttgccagg
    11161 tttcctttct agccgtgcct ttcaggccaa tgaagagcag acagatttgg aaatctacgg
    11221 acctcaagga atcaagtcat ttgtcttaac cagccttcgt gtgtcaggtt ctcgtctgcc
    11281 ctaccgcatt catttccatg agtttgacca agattctctg ggtaaaattc ttgaaaccga
    11341 taaattcact gtgtatgcag aggagctgga ccacactatt ttctgtgttg gctatcgtgt
    11401 catgcaaaag gatctagaag ggacgctgga tgctgaaaaa ctcaaggctg ctggtgttcc
    11461 gttcggcccg ctttttggta aaatcaaaaa cggccaggat cttgttttgg aagacggaac
    11521 tgaaatcaag gcagcagact atatctcagc gccacgtcca ggtaagatta tcactatttt
    11581 aggagacact cgaaaaacgg gtgccagtgt gcgtctggct gtcaatgcag atgtcctagt
    11641 tcatgagtcc acttatggca agggtgatga aaaaattgct cgtaaccatg gtcactcaac
    11701 taatatgcaa gctgcacaag tagcggtaga agcaggtgcc aaacgcctcc tactcaacca
    11761 tatcagtgcc cgtttcctct caaaagatat tagcaaactc aagaaagacg ctgccacaat
    11821 ttttgaaaat gtccatgtgg tcaaagactt ggaagaagtg gaaatctagc agtcacagaa
    11881 aggataagta tgcctactat tctcattacc ggagctagcg gtggtctagc tcaagaaatg
    11941 gtcaaactcc tgcccaatga ccaactcatc ttgcttggta gaaataagga aaaattagcc
    12001 caactctacg gaaattattc ccatgcagaa ttgattgaaa ttgatattac cgacgattca
    12061 gccctagaag ctctggtaac tgatctttat ctccgctatg gcaagattga tgtcttgatt
    12121 aacaacgctg gttacgggat ttttgaggga tttgaccaga ttgctgataa agatattcac
    12181 cagatgtttg aggtcaatac ctttgccctg atgaatctgt ctcgtcacct tgcggctcgt
    12241 atgaaggaaa gcagcaaagg gcatatcatc aacatcgtca gcatggcagg tctaatagct
    12301 actggcaagt ctagtcttta ctcagcgacc aagtttgcgg ctattggttt ttcaaatgct
    12361 ctgcgactcg aacttatgcc ctatggagtc tatgtgacaa cagtcaatcc aggtccaatc
    12421 cgaacaggat ttttcgacca agccgaccca gatggaactt atcttaaatc ggttgaccgc
    12481 ttcctgctag aaccagatgc agtggctaaa aagattgtca agattatagg aaaaaacaaa
    12541 cgagaactca atctcccgat tttgttgaac ctagctcata agttttatac cctctttcct
    12601 aagctagctg ataagttggc aggggaaact tttaattata agtaaaaaga accaatgtgc
    12661 aggttgttgc tagcctacat attggttctt atcatttctc aactatcaaa ctgaacttct
    12721 tctaggagat agtctataaa gcgttcgccc atcttagata ggctggtttt ctcatgctgg
    12781 atatagacca gctcgatcgg gtcatcaata tccagtggga tagaaacgat attgtctccg
    12841 tttaggttgc tgttcaaaat ccctgtcgca atggtataac catccaaacc aatcaagaga
    12901 ttaaagaggg tggcacggtc actgaccaca atggatttct tgtggtgttc ttgagaaaga
    12961 atctcttctg aaaagtagaa ggagttgtgc gtcccttggt catagctgag gtaagggaaa
    13021 ttctccaaat cagacagttt caccttgtct ttctttgcca gagggttggt cttgctgaca
    13081 aagatatgtg gttgcgctgt gaagagatgg tgggctagca ggtgattgtc atccagcatc
    13141 ttggttaaaa catcacggtt gtaactgttt aagaagagga ccccgacctc actgcggaag
    13201 ttcttgacgt cgtcgataat ctcccaagtc cgagtttcac gaaggaagag ttcgtatttc
    13261 tccatatcgc ttttcttgag caaagagaca aaggcattga ccacaaaggc atagtgttga
    13321 gacgaaacgc taaatagttc gcggtgggcg acaggatttt tatagcgttc ctccagaagc
    13381 tgggtctgct cgacaacctg acgggcataa gagagaaact ccatcccatc acgggtcaag
    13441 gtgattccct tgggattgcg gataaagatc tcaatgccca tttcattttc caaatctcgc
    13501 actgcattgg agagacttgg ctgagtgata aagagttgct tggctgcctc attcatggag
    13561 ccagtttcga cgattttgat aatataatgt aattgttgaa ttctcatgtt tttattgtac
    13621 catacttgcc ttagaatcgc taaagaagat aggaaaataa ggagcattgt gtgagtctct
    13681 tcttgaaaaa aacacaaaat tttgctagaa tgagtcttat gaaaacattc tatgatgtgc
    13741 agcaattcct caaacgattt ggtattattg tttacatggg aaaacgctta tatgatattg
    13801 aactgatgaa gttggaacta tctcggattt acgatgcagg gttgatggat aaattagact
    13861 atctagaggc agaagcagtt cttcgcagag agcacaaggt agaattggat tatattgaga
    13921 aaaatggaga aaagaactaa tggttacttg gattttgtgg gcacttatac tagcaatgtt
    13981 ggcgtggatg ggctttaact atcttcgtat tcgccgtgcg gctaaaattg tggacaatga
    14041 ggagtttgaa gccttgattc gtacgggtca attgattgat ttgcgcgacc cagcagaatt
    14101 ccacagaaaa catatccttg gtgcacgcaa tattccttca agtcagttga aaactagtct
    14161 tgcagccctt cgtaaagata aacctgtcct tctctacgag aaccaacgtg cgcaacgagt
    14221 tacaaatgca gctctttact tgaaaaaaca aggtttttct gagatttata tcctttctta
    14281 tggcttggat tcttggaaag ggaaagtgaa gactagctaa ccttatttcc aaattagtcg
    14341 aatgatagga tgaagctatt gttttatagt ataattaatt tgtataaaat ttaaggagtt
    14401 cttagaacat aaaagaaaag aagaaaaatc cactttttgt gggtatcttg tcgattattc
    14461 ttggtttgtt atttccaatt gtaggcctga ttttgggtat cataggattg gtcttggcta
    14521 tttcatacca aaaagagtca caattagact ataaaataga aaagattctt aatatcttag
    14581 gaattgtgat ttctgtagtt aactggattg tagcaatcgc cttaattttt cggtaaataa
    14641 aagaaaaaag ctttagtttt aaagcttttt ttgttttatc gactcatctc taaatagttt
    14701 ttaatgattt gtaactcttt ttggttcaaa gggcggtagt taccttctgc taaatctggg
    14761 ttcaatgtga agtccccaaa ttggattctt ttgaggctag tcaccttaac accaaccgag
    14821 aggaacattt tcttgatttg atgaaatttt ccttctgaaa tggtgataga ggcttgactg
    14881 agggagggac ttgcagatag aatctctagt tttgcgggtt tacagacagt gtcatctaaa
    14941 aagacaattc ctttttgaaa ggtttggata tggtcaggtg ttagaagtcc attaacctca
    15001 acttggtaag tcttatcgac atgatattgg ggatggagga gctgaaagcc caagggaccg
    15061 ttatcggtca agaggaggag tcccgttgta tctcggtcca gtcggccaac ggcatagagc
    15121 ttgtcagact ggatgttaga tggaagcagg tccatgacgg tcggaagttc cttgtctttg
    15181 ttggctgtaa cggcaccagc aggtttatga agcataagat aggtgtgttc atagccttga
    15241 atgattcgat cctgaaaaag gagttcttgt agtcctgtat cgatattttg agctagggag
    15301 cgggctgggc aaccatcgac tagaatttcc cctctgagga gtgcttgctt catggccttt
    15361 cggctgattt tttcttgagc taataaatta tctaaacgca tactgtgcct atcttatcat
    15421 aagtcgctcg ctttgaaaag gcttgacgtg aaaagaaaag cagagtgaaa acatttacac
    15481 ttgcttgagt tatgttattt atttgaaatt atggtataat cgttcagtta gaaaataaat
    15541 tttgaatatt atagaggaaa tcatgacaaa attaagagaa gatatccgta acattgcgat
    15601 tatcgcccac gttgaccacg gtaaaacaac cctggttgac gaattattga aacaatcaga
    15661 aacgcttgat gcacggactg aattggcaga gcgtgctatg gactcaaacg atatcgaaaa
    15721 agagcgtgga attaccatcc ttgctaaaaa tactgccgtt gcttacaacg gaactcgtat
    15781 caacattatg gacacaccag gacacgcgga ctttggtgga gaagttgagc gtatcatgaa
    15841 aatggttgac ggtgttgtct tggtcgtaga tgcctatgaa ggaaccatgc cacaaactcg
    15901 tttcgtattg aaaaaagcct tggaacaaga ccttgtccca atcgtggttg ttaacaaaat
    15961 cgataagcca tcagctcgtc cagcagaagt agtggatgaa gtcttggaac ttttcatcga
    16021 gcttggtgca gatgacgacc agcttgattt cccagtggtt tatgcttcag cgatcaacgg
    16081 aacttcttca ttgtcagatg atccagctga ccaagaagcg actatggcac caatctttga
    16141 cacgattatc gaccatatcc cagctccagt agataactca gatgagcctt tgcagttcca
    16201 agtgtcactt ttggactaca atgacttcgt tggacgtatc ggtatcggtc gtgtcttccg
    16261 tggtacagtt aaggttgggg accaagttac cctttctaaa cttgacggta caactaaaaa
    16321 cttccgtgtt acaaaactct tcggtttctt tggtttggaa cgtcgtgaaa tccaagaagc
    16381 caaagcgggt gacttgattg ccgtttcagg tatggaagac atctttgtcg gtgaaaccat
    16441 cactccgaca gatgcagtag aagctcttcc aatcctacac atcgatgagc caactcttca
    16501 aatgactttc ttggtcaaca actcaccatt tgctggtaaa gaaggtaaat gggtaacttc
    16561 tcgtaaggtg gaagaacgct tgcaggcaga attgcaaaca gacgtttccc ttcgtgttga
    16621 cccaactgat tcaccagata aatggactgt ttcaggacgt ggagaattgc acttgtcaat
    16681 ccttatcgaa acaatgcgtc gtgagggcta tgaacttcaa gtatctcgtc cagaagttat
    16741 cgtaaaagaa atcgacggta ttaaatgtga accatttgaa cgtgtacaaa tcgacactcc
    16801 agaagaatac caagggtctg ttatccaaag cctttctgaa cgtaaaggtg aaatgttgga
    16861 tatgatttca actggtaatg gtcaaactcg tttggtcttc cttgttccag cgcgtggttt
    16921 gattggatac tcaactgagt tcttatcaat gactcgtggt tacggtatca tgaaccatac
    16981 cttcgaccaa tacttgccat tgattccagg ggaaattggt ggacgtcacc gtggtgccct
    17041 tgtttctatc gatgctggta aggctacaac ttactcaatc atgtctatcg aagaacgtgg
    17101 tacgatcttt gtcaacccag gtactgaggt ttatgaagga atgatcatcg gtgaaaactc
    17161 tcgtgaaaat gacttgacag ttaacatcac gaaggcaaaa caaatgacca acgttcgttc
    17221 agctactaag gaccaaacag ctgttatcaa gacgcctcgt atcttgacac ttgaagagtc
    17281 tcttgagttc ttgaacgacg atgagtacat ggaagtaacg cctgagtcta tccgtttgcg
    17341 taaacaaatc cttaacaaag cagagcgtga gaaagctaac aagaagaaaa aatcagctga
    17401 ataagagcta gaaagagaga aagatggtct atttagtcct aggaatttta ctgctcctac
    17461 tctatgtatt tgcgacacca gaaagcatta aagggactgt caatatcgtc gctatggtat
    17521 gtattttagt ggcactcttg attttattgg ttctatcttt tctgaaaatt tttcaattac
    17581 caacagaaat attcctagca atagccatgt tgatcctagc ttactttagt gttagagaca
    17641 tcacactcat gccagtcaaa aaaagtaaaa gaagataaaa aagagagttt cggctctctt
    17701 tttgctagga attttacaat agtttgaatt gctttttagt taacaccatc tcttttactt
    17761 gtatagtatg aatgggaaaa acttgaataa aattcgttaa tggcatctat tttatatttg
    17821 gttagtactg gttgcatatt attttgaaat aaaactacta tataatatta ttcacagagg
    17881 taatatagta aaatatctaa aaaattcgat tttttccttg acaacaaata ataaaaaagt
    17941 tattcttatc acataagaaa gcggtttcaa taaaagagag aggaggattt attttgttgc
    18001 attgttgctc aaggtgtgct ttttttgaaa gaaaaaataa aatgaaaacg aaaaaacata
    18061 gattacttgc tctagctctt atttcaagtt ttacattatt gggagctgca tcagctgctg
    18121 tataatatcc agatggagga gtatggacat atggagaagg ttcaggaggt ggttaggctt
    18181 tttcaaatta ctatcatggt aaaaaatatc attattcatc tatcgtgagc agatgggatg
    18241 gtcattctga taaaggagaa gctcctgctg gaaaaacctc ctatgcatgg atttggacta
    18301 aatggggaga acaagtagca ttttactgtg attatgacta atcgttcatt gatttaaatc
    18361 gttccccttg taagaaatta cagggggaat tttgtttaca aggagtctac tatgaagaaa
    18421 ctattcatat tattatcaac tttttttctc agcttcttcc ttgcttggat tattgtctta
    18481 cgtgcgccac aatatttata tgcaagctat gattccgttt ctttacttcg tgtcaaaaaa
    18541 gatactcagg aaccgacgcg tgaggtattt gaacaggaat tggagaattt tgcaaactca
    18601 gaacagagtt taatagctag aagaattgta gagccgagta aggatgggac gactcacttt
    18661 acttatgcaa cttatggtca gggaacttta ccaaaagaat tccaagaagc tagccaagaa
    18721 agtcgtgaac gtagtgatcc gctaaatagt tatctccttt tgtcaggctc cttgacgaaa
    18781 gaaaagcttg ccgataaatt aggagatttg ggttataaag caagtgctga ccgaaagata
    18841 ccgccctatt ttcttgcttt tcgaatatta ctaaatcccc ttattttaat tagtttagca
    18901 atatttggct tatctttctt tgctttagtg attatcactc ggattaagga aatgagagca
    18961 gcaggtataa aactcttttc tggtcagact ctcttatcca tcatggggca ttctttatct
    19021 actgatatca aatggctcct tctatcagcc ctcctttcct tcctaggtgg gggagtcgtt
    19081 ctttttagtc aaggtttgtt ttatcctatc ttgttagcca cctatggttt tgggattagt
    19141 ttctatctgt tgtttttatt ggcgatttca attttactaa tgctccttta cctaatgagt
    19201 ttgagttaca aagcattagt tcccgttatt aagggaagat taccccttaa acgcttgatg
    19261 gctttaaccc tattgtgtca gttagtagct gtttttacag taggctacgc tgttaagaca
    19321 ggtttgacgt cttaccaacg attgaaagaa cttgaaattt caaaacaagc atggcaggac
    19381 agagcagact attaccagat ttcctttggc ttaggtgata gaggaaaaga tacagaaaat
    19441 cagagcaagt ggtatgcctt tgccaaggaa gcaatcgaag aagaacaagc tctttatgta
    19501 aaggataatc tgctccattt tgccaatcca caaggaaaaa atgaacaggg agagacactg
    19561 gatacctata gtccagatgc taatacgctc tatgttagtc ccagttattt ggacaaggaa
    19621 aaggtcgtgg tagatgctga gaccaaacag aagttagccc atctccaaaa aggtgagttt
    19681 atcctcttgc tcccagaaca tttgcgctct cgagaagcag aacttaagaa agtttttgaa
    19741 gaaagattga gttattatgg aaaatctggt gaggaggcaa gtgctccttt ggattatgag
    19801 atgaaagcgc atgttagtta tctttcaatg ggagaaaagc ggtttgttta taataacggt
    19861 gagaatcccg tatctactca gtatttgact gatccgattt tagtggtatt cacgccgact
    19921 tctacaggtg atagttttat atccttatct agttggtcta tcaatgctgg aaaacaactc
    19981 tttatcaaag gatatgagag tgggctagaa ctcttgaaga aagctggaat ttatgagcaa
    20041 gtatcctatc ttaaagaagg aagaagtgtt tatctaactc gttataatga agttcaaact
    20101 gaaacagcaa ctttaatctt aggagctatt gttggaatag ccagttcctt gttactcttt
    20161 tattctgtca atcttctata tttcgagcaa ttccgccgag atatcttgat taaacgaatt
    20221 tcaggtttac gattttttga aacacatgct cagtatatgg ttagccaatt tgctagtttt
    20281 gtatttggtg ctagtctctt tattttaagc agtcgagact tggtgattgg cttgctcact
    20341 ttattggtct ttctagctag tgcagttttg acgctttacc gtcaagcgca gaaagaatct
    20401 cgtgtttcta tgacaattat gaaaggaaaa taggatgatt gaactaaaga atatatctaa
    20461 aaaatttgga agccgtcagc tattttcaga tatgaatctt cattttgaag gtgggaaaat
    20521 ttatgcctta atcggtacaa gtggctgtgg taagacaaca ctcttgaata tgattggacg
    20581 attggagcca tatgacaaag ggcaaatcat ctatgatggc acttctctta aggacatcaa
    20641 gccttctgtt ttctttagag attacttagg atacttattt caagattttg gcttaattga
    20701 aagccaaacc gtcaaagaga atctcaatct gggtttagtt ggtaaaaagt tgaaggaaaa
    20761 agagaaaatc tctttgatga aacaagctct aaaccgtgta aacctctctt atttggattt
    20821 gaagcaacct atatttgagt tatcaggagg agaagcacaa cgtgttgcac tagcgaagat
    20881 aattttaaag gatccacctt tgatcctcgc agatgaacca accgcttcct tagaccccaa
    20941 aaactctgag gaattacttt ccatcctaga atctttaaaa aatccgaatc ggaccattat
    21001 tattgcgacc cataatcctc tgatttggga gcaagtggat caggtcattc gagttaccga
    21061 tttatcacat agatgatatg gtaagattca gttagaagaa agagtcacaa acacactttg
    21121 tggctttttt atttccataa aaatggtaaa atagtaggag tagaaatgga gttcgagaca
    21181 tgaaagtaat agatcaattt aaaaataaga aagtccttgt tttaggtttg gccaagtctg
    21241 gtgaatctgc agctcgtttg ttggacaagc taggtgccat tgtgacagta aatgatggga
    21301 aacctttcga ggacaatcca gctgcccaaa gtttgctgga agaagggatc aaggtcatta
    21361 caggtggcca tcctttggaa ctcttggatg aagagtttgc ccttatggtg aaaaatccag
    21421 gtatccccta caacaatccc atgattgaaa aggctttggc caagggaatt ccagtcttga
    21481 ctgaggtgga attggcttat ttgatttcag aagcaccgat tattggtatc acaggatcga
    21541 acggtaagac aaccacaacg actatgattg gggaagtttt gactgctgct ggccaacatg
    21601 gtcttttatc agggaatatc ggctatccag ctagtcaggt tgctcaaata gcatcagata
    21661 aggacacgct tgttatggaa ctttcttctt tccaactcat gggtgttcaa gaattccatc
    21721 cagagattgc ggttattacc aacctcatgc caactcatat cgactaccat gggtcatttt
    21781 cggaatatgt agcagccaag tggaatatcc agaacaagat gacagcagct gatttccttg
    21841 tcttgaactt taatcaagac ttggcaaaag acttgacttc caagacagaa gccactgttg
    21901 taccattttc aacacttgaa aaggttgatg gagcttatct ggaagatggt caactctact
    21961 tccgtggtga agtagtcatg gcagcgaatg aaatcggtgt tccaggtagc cacaatgtgg
    22021 aaaatgccct tgcgactatt gctgtagcca agcttcgtga tgtggacaat caaaccatca
    22081 aggaaactct ttcagccttc ggtggtgtca aacaccgtct ccagtttgtg gatgacatca
    22141 agggtgttaa attctataac gacagtaaat caactaatat cttggctact caaaaagcct
    22201 tgtcaggatt tgacaacagc aaggtcgtct tgattgcagg tggtttggac cgtggcaatg
    22261 agtttgacga attggtgcca gacattactg gactcaagaa gatggtcatc ctgggtcaat
    22321 ctgcagaacg tgtcaaacgg gcagcagaca aggctggtgt cgcttatgtg gaggcgacag
    22381 atattgcaga tgcgacccgc aaggcctatg agcttgcgac tcaaggagat gtggttcttc
    22441 ttagtcctgc caatgctagc tgggatatgt atgctaactt tgaagtacgt ggcgacctct
    22501 ttatcgacac agtagcggag ttaaaagaat aatatgaaaa aaattgtctt tacaggtggg
    22561 gggacggttg gacacgttac cctcaatctt ttgttaatgc ccaagttcat cgaagatggt
    22621 tgggaagtcc actatatcgg ggacaagtgt ggtatcgaac accaagaaat ccttaagtca
    22681 ggtttggatg tcaccttcca ttctattgcg actggaaaat tgcgtcgcta tttctcttgg
    22741 caaaatatgc tggacgtctt caaagttggt tggggaattg tccaatcgct ctttatcatg
    22801 ttgcgactgc gtccacagac ccttttttca aaggggggct ttgtctcagt accgcctgtt
    22861 atcgctgcgc gtgtgtcagg agtgcctgtc tttattcacg aatctgacct gtctatgggc
    22921 ttggccaata aaatcgccta taaatttgcg actaagatgt attcaacctt tgaacaagct
    22981 tcgagtttgg ctaaggttga gcatgtggga gcggtgacca aggtttcgga caaaaacact
    23041 ccagaaccag atgaattggt ggatattcaa acccacttta atcccaaatt gccaactgta
    23101 ttgtttgttg gcggttctgc aggcgctcgt gtctttaacc aattggtgac agaccataag
    23161 aaagaactaa cagagcgcta caatattatc aatctaactg gagattctag tctgaatgag
    23221 ttgaggcaaa atctttttcg tgttgactat gtgaccgatc tctatcaacc cttgatggaa
    23281 ttggctgata ttgttgtgac acgaggtggt gccaatacga tttttgagct cttggcgata
    23341 gcaaaattgc atgtcattgt gccgcttggt cgtgaagcta gtcgtggtga ccagattgaa
    23401 aatgcagctt actttgttaa gaaaggctat gcagaagacc ttcaagaaag cgatttgacc
    23461 ttggatagtt tggaagagaa gctttctcac ttactaagtc acaaggaaga ttaccaagct
    23521 aagatgaaag cttctaagga attgaaatct ctagcagatt tttatcaatt gttgaaaaaa
    23581 gatttatcat aaggaaagta aatgtcaaaa gataagaaaa atgaggacaa agaaaccctc
    23641 gaagaattga aagagttatc agaatggcag aaacgaaacc aagaatatct aaaaaagaag
    23701 gctgaagaag aggcggctct agctgaggag aaggaaaagg aaagacaagc tcgaatggga
    23761 gaagaatctg agaagtcaga ggataaacag gaccaggaga gtgaaacaga ccaggaagat
    23821 tcagaatcag ctaaggaaga gtctgaagaa aaagtagcat cctcagaggc tgacaaagag
    23881 aaagaagaga aagaagaacc agagtctaaa gagaaggagg aacaggataa aaagcttgct
    23941 aaaaaggcta caaaggaaaa accagccaaa gcaaagattc ctggtatcca tatcttgcga
    24001 gccttcacga ttttatttcc aagtctgctt ctattgtttg tctctgccta cttgctcagt
    24061 ccttatgcga ccatgaaaga tattcgtgtt gagggaacgg tgcaaactac agctgatgat
    24121 attcgacagg cttcaggcat tcaggattcg gattatacga ttaaccttct gctagacaag
    24181 gcaaaatatg aaaagcagat taagtctaac tattgggttg aatcagctca acttgtctat
    24241 caatttccaa ctaagttcac tattaaggtc aaggaatatg atattgtggc ctactatatt
    24301 tctggtgaaa atcattatcc tattctttcc agtggtcagc ttgagactag ttctgtgagt
    24361 ctgaacagtt taccagaaac ttatttatca gttctcttta atgatagtga acaaatcaaa
    24421 gtttttgtct cagaacttgc tcaaattagc ccagaactca aggcggctat ccaaaaggtg
    24481 gaattagccc caagcaaggt gacatccgat ttaattcgat tgaccatgaa tgattcggac
    24541 gaagtcttgg ttcctctatc tgaaatgagt aagaaactgc catattacag taagattaag
    24601 cctcaattgt cagaaccgag tgtggtcgac atggaagctg gaatctacag ctacactgtg
    24661 gcggataaat taattatgga agctgaggaa aaagccaaac aagaggccaa ggaagctgag
    24721 aaaaaacaag aagaagaaca gaaaaaacaa gaggaagaga gcaatcgaaa ccaaacgacc
    24781 cagcgttcat cgcgtcgcta ggtttacctt ttctcttata gttctttagt gaccatgttt
    24841 ttacgtttaa tatttgacat ctgtttttct ttatgttaca tctgcaatgt aatcgattac
    24901 aaaataattt ttgatgaaga aggtaacaca tatgtcagat gaagtattct tatttgaaga
    24961 aattgaagaa atcgtagccc ctacagatgg tgaatttttg ggggaagttt tacttggaac
    25021 tggggtagtt ctcttaattg gagtagcctg ttgttaaaaa gatagggagt gataatcatg
    25081 caagataact ttttatttga ggaaattgaa gaaatttcag taccagttag tgatttttca
    25141 gctggacttg caacaggtat cggatttggt ttagcaatcc ttgctcttgc tggttgttga
    25201 agtttgttca tttactaaca tcaagctttt tcaatttcat tttagacagt catttaaatt
    25261 ttccgtatta gtcttgcagc aagagattaa tagaattagt cattatttta ttgattgcgg
    25321 actgagggac tagagtatgt tttacttaac ccctctttta tttattaaag gttaggtttg
    25381 ttatgagaat tgttgataag attaagatat tacctactcc ttatgaggga cactatcatt
    25441 tatatatacc atccagtaag aaacatgtat tagttgggaa acaggaaaaa aatggttaga
    25501 gcaactaata ggtcaagaat ttaccatatc ggacttatta gtgttagtag ggaagaaata
    25561 tttttaaaa
//
LOCUS       DAVHEX010000028        25444 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVHEX010000028 DAVHEX010000000
VERSION     DAVHEX010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25444
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.028"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(<1..237)
                     /locus_tag="VVL45_001137"
     CDS             complement(<1..237)
                     /locus_tag="VVL45_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEU4187321.1"
                     /translation="MLAVLKTAYQLKHAKGGRKPKLSLEDLLMATLQYVLEYRTYEEI
                     AADFGIHESNLIRRSQWVEVTLVQSGVTISKTHLS"
     gene            complement(1321..1524)
                     /locus_tag="VVL45_001138"
     CDS             complement(1321..1524)
                     /locus_tag="VVL45_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pncT"
                     /protein_id="HEU4187322.1"
                     /translation="MEKIDYITLNEVELETISGGDDCFIGDIGCIGWGLLKSIGGMIK
                     PAPYVPPVCIPKSSWNPAPPVPC"
     gene            complement(1842..2045)
                     /locus_tag="VVL45_001139"
     CDS             complement(1842..2045)
                     /locus_tag="VVL45_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin class II family protein"
                     /protein_id="HEU4187323.1"
                     /translation="MNTKMMEQFSVMDNEMLDRIEGGIFGVDDALFWAGLGYVAGSIV
                     DTAIDDFTNQCRKNPHQWFCVRV"
     gene            2327..2914
                     /locus_tag="VVL45_001140"
     CDS             2327..2914
                     /locus_tag="VVL45_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HEU4187324.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLIIGDPDPSVKITKMSKERFFYEWTGVAIFLATKPSYQPHKDKKNGLLSKLPS
                     SDFQTKISHCLHCSLKLIGHYYQYRWFLLSPRNLG"
     gene            2859..3584
                     /locus_tag="VVL45_001141"
     CDS             2859..3584
                     /locus_tag="VVL45_001141"
                     /inference="COORDINATES: protein motif:HMM:NF012869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin ABC transporter"
                     /protein_id="HEU4187325.1"
                     /translation="MVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVITYILQQ
                     VMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDANSIIDA
                     LASTILSLFLDVSILILVEGVLLAQNPNLFLLSLISIPIYMFIIFSFMKPFEKMNHDV
                     MQSNSMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLEKSFKLSKYSILQTSL
                     KQGNKISSEYPYPMVWRSISHVE"
     gene            3574..4485
                     /locus_tag="VVL45_001142"
     CDS             3574..4485
                     /locus_tag="VVL45_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4187326.1"
                     /translation="MSSKISIGQLITFNTLLSYFTTPMENIINLQTKLQSAKVANNRL
                     NEVYLVESEFQVQENPVHSHFLMGDIEFDDLSYKYGFGRDTLTDINLTIKQGDKVSLV
                     GVSGSGKTTLAKMIVNFFEPYKGHISINHQDIKNIDKKVLRRHINYLPQQAYIFNGSI
                     LENLTLGGNHMISQEDILKACEVAEIRQDIERMPMGYQTQLSDGAGLSGGQKQRIALA
                     RALLTKSPVLILDEATSGLDVLTEKKVIDNLMSLTNKTILFVAHRLSIAERTNRVIVL
                     DQGKIIEVGSHQELMQAQGFYHHLFNK"
     gene            4496..5857
                     /locus_tag="VVL45_001143"
     CDS             4496..5857
                     /locus_tag="VVL45_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HEU4187327.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEITPTSVITSIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGISKTN
                     TEVSNQANLANSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRY
                     LIASQEQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLEKNTLTAPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     SIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
     gene            5914..6069
                     /gene="blpC"
                     /locus_tag="VVL45_001144"
     CDS             5914..6069
                     /gene="blpC"
                     /locus_tag="VVL45_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HEU4187328.1"
                     /translation="MDKKQNLTSFQELTTTELNQIIGGGWWEDFLYRFNIIEQKNTKG
                     FHQPIQL"
     gene            complement(6113..7442)
                     /locus_tag="VVL45_001145"
                     /pseudo
     CDS             complement(6113..7442)
                     /locus_tag="VVL45_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            complement(7456..8193)
                     /locus_tag="VVL45_001146"
     CDS             complement(7456..8193)
                     /locus_tag="VVL45_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4187329.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKAHHIIPSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANGQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSTRPHRVILYTKTDRLEFTASLEEVLKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINNLH"
     gene            complement(8198..8536)
                     /locus_tag="VVL45_001147"
     CDS             complement(8198..8536)
                     /locus_tag="VVL45_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4187330.1"
                     /translation="MKYMIIQTQKTVYKVNIDDIYYIQTHPTKAHTVQIVTEEASFNM
                     LQNLSNLENQCGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            8813..9142
                     /locus_tag="VVL45_001148"
     CDS             8813..9142
                     /locus_tag="VVL45_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEU4187331.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(9310..10359)
                     /locus_tag="VVL45_001149"
     CDS             complement(9310..10359)
                     /locus_tag="VVL45_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4187332.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKASKFFTETG
                     LDWDYVISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     QVLYLPYQVKRQMQD"
     gene            complement(10356..11087)
                     /locus_tag="VVL45_001150"
     CDS             complement(10356..11087)
                     /locus_tag="VVL45_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4187333.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVADPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            11155..11565
                     /locus_tag="VVL45_001151"
     CDS             11155..11565
                     /locus_tag="VVL45_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU4187334.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            11575..11862
                     /locus_tag="VVL45_001152"
     CDS             11575..11862
                     /locus_tag="VVL45_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187335.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIIDQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
     gene            12024..12218
                     /locus_tag="VVL45_001153"
     CDS             12024..12218
                     /locus_tag="VVL45_001153"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187336.1"
                     /translation="MHHNLGNSYSIKIKKQTRKLGTDCSKHRFEVVDRTDEVSNHTHD
                     KPTLTWFEEIFEEYHSPFHN"
     gene            complement(12552..13688)
                     /gene="dnaJ"
                     /locus_tag="VVL45_001154"
     CDS             complement(12552..13688)
                     /gene="dnaJ"
                     /locus_tag="VVL45_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEU4187337.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(14090..14446)
                     /locus_tag="VVL45_001155"
     CDS             complement(14090..14446)
                     /locus_tag="VVL45_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine-N7)-methyltransferase"
                     /protein_id="HEU4187338.1"
                     /translation="MSVLDEEYLKNTRKVYNDFCNQADNYRTSKDFIDNIPIEYLARY
                     REIILAEHDSCIKNDEAVRNFVTSVLLSAFVSAMVSAMISLEIQTHKFVIPFIIGMIW
                     TVVVFLMINWNYIGKY"
     gene            complement(14448..16271)
                     /gene="dnaK"
                     /locus_tag="VVL45_001156"
     CDS             complement(14448..16271)
                     /gene="dnaK"
                     /locus_tag="VVL45_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEU4187339.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(16701..17225)
                     /gene="grpE"
                     /locus_tag="VVL45_001157"
     CDS             complement(16701..17225)
                     /gene="grpE"
                     /locus_tag="VVL45_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEU4187340.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(17252..18286)
                     /gene="hrcA"
                     /locus_tag="VVL45_001158"
     CDS             complement(17252..18286)
                     /gene="hrcA"
                     /locus_tag="VVL45_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEU4187341.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFDRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVHLLNFANLAAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQVNVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(18450..18959)
                     /locus_tag="VVL45_001159"
     CDS             complement(18450..18959)
                     /locus_tag="VVL45_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEU4187342.1"
                     /translation="MNSRVEFRIFTIVDLDKKEEYLHEMHLKGWRYRTSRFGLFYFEQ
                     GQPDDVIYRIYDSRFLKEHKRELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCTTTFLGGLVVCTSLFREDLSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
     gene            19567..21900
                     /locus_tag="VVL45_001160"
     CDS             19567..21900
                     /locus_tag="VVL45_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU4187343.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFRPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKVIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDANGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGDPVKVLE
                     TGAKTVSGSTPGFVDEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            21913..23376
                     /locus_tag="VVL45_001161"
     CDS             21913..23376
                     /locus_tag="VVL45_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEU4187344.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSIM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLNPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            23376..24945
                     /locus_tag="VVL45_001162"
                     /pseudo
     CDS             23376..24945
                     /locus_tag="VVL45_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187251.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            complement(24893..25438)
                     /locus_tag="VVL45_001163"
     CDS             complement(24893..25438)
                     /locus_tag="VVL45_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU4187345.1"
                     /translation="MNWVVIKIKDIFSMNTGLSYKKGDLSINNKGVRIIRGGNIKPLE
                     FSLLDNDYYIDTQFISSEQVYLKHNQLITPVSTSLEHIGKFARIDKDYDGVVAGGFIF
                     QLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQALYNIPKTTLSELLIPLAPFE
                     EQELITQKVEKLFEKVNQLWK"
BASE COUNT         8227 a         5033 c         4569 g         7615 t
ORIGIN      
        1 actaagatga gtttttgaaa tcgtaacacc actttgaaca agagttactt caacccattg
       61 gctccgacgg attaagttgc tttcgtggat accaaaatca gccgcaattt cttcataagt
      121 gcggtattct agcacatatt gaagagtggc cataagaagg tcttctaggc ttaatttagg
      181 ttttcgtcca ccttttgcgt gtttaagttg ataagctgtt tttaatacag ctaacatctc
      241 ttcaaaagtc gtgcgctgaa caccaacaag acgcttaaat cgtgcatcag ttagttattt
      301 acttgcttca taattcatag aactatagta aaatgaaata agaacaggat aaatcgatca
      361 ggacagtcaa atcgatttct aacaatgttt tagaagtaga ggcgtactat tctagtttca
      421 atctactata ctataccata ttttgtttcg cagggaatct attataaaag ggtaagtatt
      481 tcaaaaacac ttaccccttt atactttatt aagctctact ttttataata cttcaatccc
      541 caaatgaaag gaagatgttc cgttttttag caagaaaagt tttcatttct ttgctccttt
      601 ctttttagtt aaagcaaaat agatcataac tgcaatcaca taggctatgg tataaaatag
      661 ctgataccaa atactttccc taagcggata aagaaagatg gacatgatta gatacaagat
      721 gaaaatgata agtatttttt tcttcataaa atttcctcct aaatgtgtga tttatcttag
      781 ttgaacaaga atctttacac tgctagtata gcactcattt tgaccttgga ttactcaaat
      841 catgaatggt catcaaaaca tcttgaacaa taacaagatt ttcatctata caaaaatagt
      901 tatactcaac aacaatcaaa ctaaagaacc aaataaatat agcttaacat actttttttg
      961 gatttcaaac aatactgata aaattaataa taaatctgtg gtagtacttc ccatccctac
     1021 tgctttccaa ttttataata ttattaatta aataatatat gagaaaaaac gaacaagact
     1081 gtgttaataa tctatcttat tcgttttatt tattagactt gttcagaaat tatttgaagc
     1141 agtattagct ggttagaaag tgaattttta acgttccaac cagccattag tttctagttt
     1201 tcgactcttg caataagcta aaataatcct gaacaacaca tgtatattat agttgcgaag
     1261 attcaacaaa atagcgaaca agtcgattat tcatttttta tataagttta caataacttt
     1321 ttaacatgga actggaggtg caggattcca cgaacttttt ggaatgcata ctggaggaac
     1381 atatggagca ggttttatca tccctccgat actcttcaaa agcccccagc cgatacatcc
     1441 gatatctcca ataaaacagt catctccacc actaatcgtc tctaattcta cttcattcaa
     1501 agtaatataa tcaatttttt ccattgttat aattcattcc tttcgtttta ctcaaggcac
     1561 aacacagaat gaaaaagtgt tgtactcttt attttataat gtttataata atagtgagaa
     1621 aacctatcac tactacaaat cacggggagg tgaataagtg agtgatatag ccaatatctc
     1681 gcatttttat aggtgtcatt ctttctatca agcacaagaa ctgtgactag gagcacgtaa
     1741 acttataaca gaataagcga ctcgtttgca aaagaatagt ttctatcact gcataggtca
     1801 ttaagttata taaattctta tacaatacaa cactactata tctatacacg aacacagaac
     1861 cactgatgag gatttttccg gcattgattc gtaaaatcat caattgctgt atcaacaatg
     1921 ctacctgcaa catagcctaa ccctgcccaa aacaaagcat cgtcaactcc aaatattcct
     1981 ccctctatcc tatcaagcat ttcattatcc ataacagaaa attgttccat catttttgta
     2041 ttcatgacaa atactccttt tttctttttt tatttttgtc ttgttgtaac tttgataagt
     2101 ttagtatatc atcgtttttt aaaatttttc atccagatct tgaattgtca tcgaaacgtc
     2161 ttgaattagc ttttttattt caagccacct ctaaatgttt aaaaaaaata atttctaatc
     2221 acttttttac cattcaggaa gttttaatga ctattcaaga tttcataaaa tatgaactta
     2281 gttttatgac ataatagacc tatccactat atgaaaggaa ttgccaatga cttcttataa
     2341 acgtacattt gttcctcaaa tagatgcgag agactgtggt gtcgctgcct tagcctcgat
     2401 tgctaaattc tatggttcag atttttctct agctcacttg agagaacttg caaagaccaa
     2461 taaagaaggg acgactgctc ttggcattgt aaaagccgct gatgaaatgg gctttgaaac
     2521 aagacctgtt caagcagata aaacgctctt tgacatgagt gatgtcccct atccatttat
     2581 cgttcacgtt aacaaagaag gaaaactcca acattactat gttgtctatc aaacaaagaa
     2641 agactatctg attattggtg atcctgaccc ttctgtaaaa atcactaaaa tgtcaaaaga
     2701 acgctttttc tatgaatgga ctggagtagc tatttttcta gctaccaaac ccagctatca
     2761 accccataaa gataaaaaga atggtctact aagcaagctt ccttcctctg attttcaaac
     2821 aaaaatctct cattgcttac attgttctct caagcttatt ggtcactatt atcaatatag
     2881 gtggttctta ctatctccaa ggaatcttgg atgaatacat tccaaatcag atgaaatcaa
     2941 ctttaggaat catctcagtt ggtctggtta tcacctatat cctccaacaa gtcatgagct
     3001 tctccagaga ttatctccta accgttctga gtcagagatt aagtattgat gtgattttat
     3061 cctatattcg ccatattttt gaacttccca tgtctttctt tgcgacacgt cgtacaggag
     3121 aaatcatttc acggttcaca gatgctaact ctattataga tgccttggct tctaccattc
     3181 tttctctttt tctggatgtt tctattctga ttcttgtaga gggagtcttg ctggcacaaa
     3241 accctaatct cttccttctt tctcttattt ccattcctat atacatgttc atcatctttt
     3301 cttttatgaa gcctttcgaa aaaatgaacc atgatgtcat gcaaagtaat tctatggtta
     3361 gctctgccat tatcgaagat atcaacggga ttgaaactat aaagtcgctc acgagtgaag
     3421 aaaatcgcta tcaaaatata gacagcgaat ttgtagatta tttggaaaaa tcctttaagc
     3481 tcagtaaata ttctatttta caaacgagtt taaagcaggg gaacaaaatt agttctgaat
     3541 atccttatcc tatggtttgg cgctcaatta gtcatgtcga gtaaaatttc tatcggtcag
     3601 ctgattacct ttaacacact tctttcttac tttacaactc ctatggaaaa tattatcaac
     3661 ctccaaacca aactccaatc tgcgaaggtc gctaataacc gtttgaacga agtctatcta
     3721 gtcgaatctg aatttcaagt tcaagaaaac cctgttcatt cacatttttt gatgggcgat
     3781 attgaatttg atgacctttc ttataagtat ggttttggac gagatacctt aacagatatt
     3841 aatctcacga ttaaacaagg agataaggtt agcctagttg gagttagtgg ttctggtaaa
     3901 acaactttag ccaaaatgat tgtcaatttc tttgaaccct acaaagggca tatttccatc
     3961 aatcatcagg atattaaaaa cattgataaa aaagtcttgc gccgtcatat taattaccta
     4021 ccccaacaag cctatatctt taatggctct attttggaaa acttaacctt gggcggtaat
     4081 catatgatta gtcaagaaga tattctaaaa gcttgtgaag tagctgaaat ccgtcaagac
     4141 attgaaagaa tgcctatggg ctatcaaact cagctctctg atggagctgg tctatcagga
     4201 ggacagaagc aacgaatcgc tctcgctcgt gctcttttaa ctaaatctcc tgttttaata
     4261 ctagatgaag ctactagcgg tcttgatgtc ttgactgaga aaaaggttat agataatctt
     4321 atgtccctaa ctaataaaac cattctcttt gtagcccatc gtctcagtat agccgaacga
     4381 actaaccgtg tcattgttct tgaccagggg aaaatcattg aagttggtag tcaccaagag
     4441 ttaatgcaag cgcaaggatt ctaccatcat ctattcaata aataaggaga atgtcatgaa
     4501 tcctaatctt tttagaagtg tcgagtttta tcagagacgt taccataact atgcgacagt
     4561 attaatcata cctctgtcat tactgtttac tttcatcttg attttctccc ttgttgccac
     4621 aaaagaaatt actgttacat cgcaagggga aatcacccct acaagtgtca ttacatctat
     4681 tcagtcgacc agtgataatc ctatcctagc taatcattta gtggcaaatc aagtagttga
     4741 gaaaggtgac ttactcatca aatactctga aacaatggaa gagagtcaga aaactgcctt
     4801 agcaactcaa ttacaaagac ttgagaagca aaaagaagga cttggaattt tgaaacaaag
     4861 cttagaaaaa gcgactgatc ttttttctgg cgaggatgag tttggctacc ataatacctt
     4921 tatgaatttt actaaacaat cccatgatat tgaactcggt atctcaaaga ctaacactga
     4981 agtttcaaat caagccaatc ttgccaatag cagttcatca gccatcgaac aagaaattac
     5041 aaaagttcaa caacaaattg gagaatatca agagctgaga gatgctatca taaataacag
     5101 agctcgctta ccaactggca atccgcacca gtcaatttta aatcgttatc ttatagcctc
     5161 tcaagaacaa acacaaggaa cggcagagga gccattttta tctcaaatta atcaaagtat
     5221 tgcaggtctt gaatcatcta tcgcaagcct caaaattcag caagctggta tcggaagtgt
     5281 agcaacttat gataatagtt tagcaaccaa aattgaagta ctccgcactc aatttttaca
     5341 aacagcttct cagcaacaac taaccgtgga gaatcaatta acagaattaa aagtacaact
     5401 agatcaagcg acacagcgct tagaaaagaa taccttaacc gctccaagta aaggtatcgt
     5461 tcatctgaac agcgaatttg aaggtaaaaa tagaattcca actggtacag aaattgctca
     5521 aatattccct gtcatcacag atacaagaga agtactaatc acttactacg tatcatctga
     5581 ctatctcccc ttactagata aaggacaaac tgtaagatta aaactggaga agattggaaa
     5641 tcacggcacc accatcatcg gccaacttca gagtattgat caaactccta ccagaacaga
     5701 gcaaggcaat ctctttaaat taaccgctct tgcaaaacta tctaacgagg atagtaaact
     5761 catccaatat ggcttacaag gtcgcgtcac tagtgtaact acaaagaaaa catattttga
     5821 ttatttcaaa gataaaattt taacccattc tgattaattt tcagataaca ctctataact
     5881 atttattatc ttatcaaaaa ggagaatcat aacatggata agaaacaaaa cctaacttca
     5941 tttcaagaac taacaactac cgaactcaac caaattatag gtggaggatg gtgggaagat
     6001 ttcttatata gatttaatat aattgaacaa aaaaatacaa aaggatttca tcagccaata
     6061 caactataaa aaaacaagac caagagaacg cactctcggt cttatttttt cttcattcta
     6121 tatgtattac agtaagtacc tgacgaaaaa cgtgatcttg gcaggtggta ttgagactgg
     6181 tattgggatg actttccaca attttcataa cggtatagag accaactcct ctctcctccc
     6241 ctttagaact tgctccaaag gagaagattt cagaaatatc gatgccctct tctttgatgg
     6301 aattttcaat gataaaggtc tcctgtgctc cattttttaa aaaggcaatt gaaacatgag
     6361 gttgacaggc ttctacacta gcttcaatag cgttgtcaca aaggatagac acaacggtta
     6421 gaaaatcaag tagactcatc ccctcaacct gaatctcctc aggaacttca acattaaaga
     6481 caatgttctt ttctctagct tttataaatt ttccagctag gagacttttg agggctttat
     6541 cacgaatatt caccaatcga ccaaggtcat atttattgtc ctgcaatttt tcactagaat
     6601 cctttaagac cgagtcgtag atctctttta tctgctccat atcctcctct tcaatgccca
     6661 gacgtaagct agtcaagagg ttggtgtagt catggcgaaa actccgtact tccttataaa
     6721 gctcctctat atgccgacta tagcgttcca tctctctgta gcgcaagtcc tgctcttggt
     6781 tcagtctctc atggagtttg tccttcaaat aggtatccaa tttcttgata atccccataa
     6841 aaaagagcag gtaaaagact aggatgagat ggcgaacagt cgttgattga atcccctgtt
     6901 catattcaaa gtaagacaga ttttgtatca ccaaatagta agcccccatt atccagttaa
     6961 tctgagtcag ggacttttga aaatctttat cgagaatccc ctttctcaag ctagtgaaat
     7021 catagtccaa ccatttcaaa aaagctagag aaatgaagaa attgaaaatt attatacata
     7081 accaaataaa tgagtagtca tcatatactt gcccttgtcc caaaaatgga agcacaaaat
     7141 aggaaacacc tctataaaag agattcacta atatcattgg aaagagacca taaaagagaa
     7201 ggagtttttt aggaagttct cttaaataag cctatgccgt acaagggttc cataaaataa
     7261 gataggtaaa catttcctac catatagcca agcattccaa aaacaacggc caacagtatc
     7321 ttaaaaagaa aggccttaaa aatcctctca aaagtgagac taattccatc taccttaaag
     7381 aatatgacaa tttttaatcc gtgagtaaca agtgtataca acaatatcca agcaacattc
     7441 ataaactctc ctagctcagt gtaagttatt aatagcctca gacacttccc tgaccttata
     7501 acgcgcgatt agacaacttc caccattggg aaagaaaagc agtttttctt tcttatctaa
     7561 atgcaccaca ttggcaggat tgatgagaaa agagcgatgg cactgcaaga gacgaggctc
     7621 ctgcttgaga acctcctcta gactcgctgt aaattccagc ctgtctgtct tggtatagag
     7681 aataacacga tggggtcttg tggacgtttc gagatagtaa acctctttaa aaggatactg
     7741 gaattgagca aattttgatt taaagtaaaa gcaatcttcc gctagacttt tactgtcttg
     7801 accattggca tagaggaggg ctgtctcgat acgagattca aactcctctg ccgaaagggc
     7861 cttatcaata tagtccagag cagacacttg gtagcgaaag gacaggggca taaactccga
     7921 gtgagtcgtc acaaagacaa tcagggcata cggatcccga tcccgaatct ttctagccac
     7981 ttccagtccc ttcatctctt catttcgaat ctcaatatcc aaaaagaata gctgatgggc
     8041 ccccttctca tgcacctctg ccagcagttg gtccggcttg ccaaatacct caaaagagct
     8101 aggaatgata tgatgtgctt tcaaaagttt ctcaatcgtc gtttcaattc tagtctgttg
     8161 ggaaaaatca tcttctaaaa caaatattct catcttctta ctctccctct ttcaaccatt
     8221 tttgacgaat ttctctatag cgacgtctgg catacttgac agcgtattga ccttcttctc
     8281 cgagaaaaag gattctttct tgaaaatcaa tcgattttaa tttatcaaga ttaaccaaac
     8341 aatttcgatg acatctcatc aaggtttccc cacattggtt ctcaagatta cttaaatttt
     8401 gaagcatatt aaaactagct tcttctgtaa caatctgtac ggtatgggct ttagttggat
     8461 gtgtttggat atagtagata tcgtcgatgt ttactttata gactgttttc tgcgtctgaa
     8521 taatcatata tttcattgga ttctcctcct aagaaaagaa taacagaaaa ggtctagccc
     8581 aagcacaccc aatcctaaac agtctttgaa aactcctaaa aggattattt tctacaacat
     8641 catgatgagc ttacaactat tttatcataa aattcactca gcgcaattca agacatttca
     8701 atgacaatta aagatttgaa taaaaaaaaa agaattaaaa gtgataaact aacagtatca
     8761 aaacacagtt gccagaagta agactggcac ccagattaaa ggaggcattc ccatgacaga
     8821 tacagacccc atcaaaagag ctcatacttt gattactgac ttaaacaaag cctaccaagc
     8881 atgcaaacag gcaagcgctg acgacgtccg ctttcaggag caattaaact ctattcttgg
     8941 ttttctagcc aaggctgaaa cagtggataa tcgattcttg attgaactag aaaaatttta
     9001 ccagacttcc agtcttctca tgggactcag cgctcttgat ccagatgctc caactcgcgc
     9061 cgcttggcgg gcctatgacc gcttccactt tgaccaagtc aagaccaagt taatactcaa
     9121 tgaaaatcaa agagcaaact aggaagctag ccgcaagctg tacttgagta cggtaagacg
     9181 acgctgacgt ggtttgaaga gattttcgaa gagtataagt ctctacggac caaccattat
     9241 cctgtagtaa caaacaagaa gctgagacag actgaactca acttcttttt tagtgtcgta
     9301 tcagcaatgt tagtcctgca tctgacgttt tacctgataa ggcaaataca agacttgtaa
     9361 aaccaaacca gctcccagta aggctagaag ggctagtttt tcttgaaagg taatcaaccc
     9421 aacaactaat tccacaagta aaacaaaact ggtcaatcct cgaactacct cctgtaagcc
     9481 tttttccttt tgccccttgt ccagcggaaa gagttgggtc aaatactggt agtcaaaggc
     9541 atgatagagg gccagcaact ggaagagcaa gaggtagtta aagagaacta ccactgctgt
     9601 cgcaatccaa gcttgctcga taaaaacctg cgccagtacg gaaagtaaga gaagacgaag
     9661 actgagagca aagaggtcgc catttcgcag ataagaacgc agatagagat tttgccaaat
     9721 cttcccaggc accttctgaa cagcctttaa gataaagtcc agataggcac gacgcttaac
     9781 gctgtttgaa attcccttga cctgcgtaaa gagggcaaag aaacgaagca agacttgctt
     9841 acgcttgctt tcttgagaaa taacatagtc ccaatccagt ccagtttcag tgaaaaattt
     9901 gctggccttt tgacgaaagt ggaaatattt tcctaccccc aataaaagca catagagcag
     9961 aaaaactggc aagccataac ccattgctaa aaataaaggc gcaaataaca gcaagaaaag
    10021 ggtctgtaca aagagccaaa agactaggga aatgccagtt tgacgcttga gatggagctt
    10081 gatttcctct tctccaacta agagaaagag cttgtctgga gcctccatat aggtggcaat
    10141 tcctccccaa agtaaaagta aaacagaggt aattcctaca aacaaaagga taggccaatg
    10201 attttcagga aaatgttgta agagttgact gtactggtag gctagaaagc ccaacagtac
    10261 aagcaggaac aagacaaagt ggtcattgag aacatagcgc agataaccaa gacactcctt
    10321 acgaaaggcc tgctttctct ttaaaaacaa gtctttcata ggtcctcctc ttttgtcaga
    10381 gccaagtaaa tatcattcaa actagcctca ggcatatcaa aggcttcgcg tagttgcagg
    10441 agattgcctt tggcacgcac ctctcccttg tgaagaatga caaaggcatc acacatcttc
    10501 tccgccgaat ccagcacgtg ggtactcatg agaatagact tgcccttttg cttctccact
    10561 tccaaaagct gaatcaaatc agaaatagcc agcggatcaa gaccaaggaa aggctcatcc
    10621 acgatgaaaa gacttggatc cgccacaaaa gcacagataa tcatgacctt ctgcttcatc
    10681 ccttttgaaa aatgaacagg gaaccagtct aatttctgtt ccaaacggaa catttttaac
    10741 aagggctcta ctcgttcgaa agccactttt tgctcaatac cgtaagccat agcaaccgtt
    10801 tcgatatgct ctctgagggt caattcctca tacagactag gcgtctcagg aatgtagcca
    10861 atctgcttgc ggtagctagt cgcatctcct tgcagagtca ggccattgat attgatggag
    10921 ccactataag gtgccaacag accgataatc tcattgatcg tcgttgattt cccagcacca
    10981 ttgagaccaa tcaaaccgac caactgccca ctttcaacag taaaggacac atctttcaaa
    11041 acaggaacat gaacatagcc acctgtcagg tttttaattt ctaacatatt ttctccaaat
    11101 ctggtataat gtagctatat tatatcaaaa ttcagtacag tagaggtaga ttttatgtca
    11161 gattgcattt tttgtaaaat catcgcaggg gaaattcctg cttcaaaagt atatgaagat
    11221 gagcaggtcc ttgcctttct tgatatctct caagtaacac taggacacac cttggtcgtg
    11281 ccaaaagaac actatcgcaa tcttttggag atggatgcta cgagcgccag ccaactcttt
    11341 gcccaagtac caaaagtagc tcaaaaagtc atgaaagtca ctaaggctgc tggtatgaat
    11401 atcatttcca actgtgaaga agtcgctggt caaacagttt ttcatactca cgttcacctt
    11461 gtacctcgct acagtgctga cgatgacctc aagattgatt ttatcgccca cgaaccagac
    11521 tttgacaaac ttgctcaagt cgctgaaacc atcaaaaacg cttaaggagt tgctatgaaa
    11581 ttatccaacc tactgctatt tgcaggagct gcagccggaa gttatctggt cacaaaaaat
    11641 cgccaaacca tcacagatga agtcttgaat accactgacc gcgttcaagc tatcaaggac
    11701 gatgtggata ttatccaaaa cagcctgcaa atcattgacc agcaaaaaga acttatcaag
    11761 gaataccaag aagacttgac ttacaagttt aaggtcttgg aaaaggatat ccagactaga
    11821 ctagctgtga taaaagaaat gcagggaact gaagataagt aaaaagagcc ccccggctct
    11881 tttttaaaac gtaaacaagt tatagtgaat tgaatttaga atagcacatc atagtttcta
    11941 aagcattgtt agagattact ttaaaatcct cttatcaatt tgttcatatt ctatttcaat
    12001 ctactatata tatcccatca actatgcatc ataatttagg taactcatac tcaataaaaa
    12061 tcaaaaagca aactagaaag ctaggcacag actgctcaaa acaccgtttt gaggttgtgg
    12121 atagaactga cgaagtcagt aaccataccc atgacaagcc gacgctgacg tggtttgaag
    12181 aaatttttga agagtatcat tcaccatttc acaactaaat cgaagacttt ctgccgtaat
    12241 gaaaacacct gaaacagctt ggtttcggct gtccggaaac tttaagactt aggttcaaag
    12301 tttaggtatg gaacttcgaa ggaggtcgct accgtccgtc attacttagg aaagtcttaa
    12361 aaaatctata aagtaaaaag agcccgacag ctctttttgt ttatactcaa tgaaaatcaa
    12421 aaagcaaact aggaaactag ccacgggctg ctcaaaacac tgttttgagg ttgtagatac
    12481 gactgacgaa gtcagtcaca tatatacggc aaggcaacgc tgacgtggtt tgaagagatt
    12541 ttcgaagagt attattctcc atcaaaggca tctttaatat ggtcaaagaa gccttttttc
    12601 tttggattta ctttcaagtc accagcagcc gcgaattctt tcaaggctac tttttggcgg
    12661 tcgttcaagc ctgtcggtgt tacgacatta acagtaacgt attggtcacc aactgcaccg
    12721 ccacgaaggc tcggtgcccc cttactacgt aggcggaact tcttaccagt ctgagttccc
    12781 tctggaataa ccaattcaac atcaccgtga acagttggaa tatctactgt atcaccaaga
    12841 gccgcttgga caaagttgag gttgagattg tagaagatag tcgttccttc acgttcaaac
    12901 ttgtcgctag cttccacaga aactactaca tacaagtcac cataaggtcc accgttaaag
    12961 cctgcttcac cttgaccagc gaggcgaatt tgttgacctg tttccacacc agcagggatt
    13021 ttcacatgta cgctatgagc ttgtttctca tgacctgtcc catgacaggt tgtacatgga
    13081 tatttgattt cttttcctcg accgtgacag acatcacagg ttacttggcg acgcatcata
    13141 ccaagaggag tctgcgtatc gacgttaatg acaccagcgc catgacagcg tccacaagtg
    13201 actggacttg tccctggctt agcaccagat ccattacatg tacgacagcc agcttcacga
    13261 tgatacttaa cttccttctc agttccgaag atagcttctt caaaggtcaa attgacacga
    13321 tactggagat catctccttg gcgaggagcg tttggattgc gcgaagaacc gcctccgccg
    13381 aagaaacttg agaaaatatc ctcaaaacca ccgaaaccac ctgccccatt gaaaccgccg
    13441 aaaccaccag ctccaccaaa accaccattg gcgcctgcag caccatactg gtcataggca
    13501 gcacgttttt ggtcgtcact caaagtctca taggcttctt gaacttcctt gtacttgtcc
    13561 tcagcaccag gctccttgtt gatatctggg tgatattttt tggaaagctt acgataagcc
    13621 tttttgattt cgtctgccga agcgtttttg gataccccca gacgatcata aaattcagta
    13681 ttgttcataa ttcaagatac aaagggcgtc aaacggacac agccatctag gagttttgac
    13741 gacggacgct tacgtcctag aaaaaactat cttttgggca cagtccgtag cccgtgttca
    13801 gttacgaaca cccttattcc tttctattga tgttctgaat cgaaccacca tttaggtcgg
    13861 gttcaattcc tttcttttat atttagtaag aaattctgaa acccgtgttc agttacgaac
    13921 actctttgtt cctttctatt gatgagacaa aacccaaaca aatgagattc cgtccctttt
    13981 tatcgaaaaa cagaggctgg gttgcaacct ctgcaaataa gtaaattaac tttattttga
    14041 ttttaaatat cctataagta cagaaatatt tatatatttt tgtctcttct tagtatttgc
    14101 ctatataatt ccaattgatc ataagaaata caactactgt ccaaatcata ccaattatga
    14161 acggtattac aaatttatgt gtttgtattt ctaatgatat catagctgat accatcgccg
    14221 atacaaatgc agacaacaat actgaggtaa caaaattcct taccgcttca tcatttttga
    14281 tacaactatc atgttcagct aatataattt ctctatatct agctaaatat tctattggaa
    14341 tattatcaat aaaatctttt gatgttctat agttatcagc ttgattacaa aaatcattat
    14401 aaacttttcg tgtatttttt agatactctt catccaatac actcatctta cttttccgta
    14461 aactctccgt ctacgacgtc atcgcctgcg tttcctgttg cttgtgcgcc ttctgctcct
    14521 tcttgagctt gttgcgctgc tgcggcttgt tcgtagagtt taacagcaag tccttgagct
    14581 ttttcgttca atgcttcaag ttttgctttc atgtcgtcca agttgttgtc ttcttgagct
    14641 ttcttaaggt catcaagggc agcttgggca gcgtcacgtt ctgcgtcgaa gcctttacct
    14701 tcagtttcct tgattgtctt ttcagtcgca aagattgctt ggtctacttc attacgaagg
    14761 tctacttctt ctttacgttt cttatcggct tcagcgtttg cttctgcatc tttcatcatg
    14821 cggtcgattt cttcgtcagt caaacctgag ttcgattgga tgacaatagt ttgttctttt
    14881 tgagttccaa ggtctttggc cttaacagac acgataccgt tcttgtcgat gtcaaatgtt
    14941 acttcgattt gaggaattcc acgaggtgca gctgggatat cagtcaattg gaagcgtcca
    15001 agagtcttgt tatctgctgc cattgggcgt tcaccttgaa gaacgtggat atcaacggct
    15061 ggttggttgt ctgctgctgt tgagaagact tgtgatttag atgttgggat tgtagtgttg
    15121 cgatcgataa gttttgtaaa tactccaccc attgtttcga taccaagtga caatggcgtt
    15181 acatcaagaa ggacaacatc cttgacatca ccagtaatca caccaccttg gatagccgca
    15241 cccatagcaa ctacttcatc agggtttact gatttgtttg gttctttacc agtttcagct
    15301 ttaacagctt caacaacggc agggatacga gttgaaccac caacaaggat aacttcgtcg
    15361 atttctgaca agctcaaacc tgcatctgaa agggcttgac gaactggaac ttttgtacgt
    15421 tcaacaaggt cacgagtcaa atcatcaaat ttcgcacgag tcaaagtcat ttccaagtga
    15481 agaggtccag cctcacctgc agtgataaat ggcaagctga tttgtgttga agttacacca
    15541 gaaaggtctt tcttcgcttt ttcagccgca tctttcaaac gttgcattgc catcttgtca
    15601 gtagacaagt cgataccgtt ttctttcttg aattctgcta ccaagtggtc aatgattttt
    15661 tggtcaaagt cgtcaccacc aagtttgttg tcccctgcag ttgacaatac gtcgaagaca
    15721 ccgtcaccca attcaaggat agagacgtcg aatgtaccac caccaaggtc aaataccaag
    15781 attttttctt ctttgtcagt cttgtccaaa ccataagcaa gagctgctgc agttggttcg
    15841 ttaacaatac gttctacttc aagaccagca attttaccag cgtcttttgt tgcttgacgt
    15901 tgagcgtcgt tgaagtaagc cggaactgtg ataacagctt tggttacttt ctcaccaagg
    15961 tagtcttcag cgtagccttt caagtattga aggatcatag ctgagatttc ttgtggagtg
    16021 tattcttttc catttgcaga aactttttca gaagttccca tcttagattt gatagagata
    16081 actgtatctg ggtttgtgac tgcttgacgt tttgcagcat caccaacgat gatttctccg
    16141 tttttgaatg agactacaga tggagttgtg cggtttcctt ctgggtttgc gatgattttg
    16201 ctttcagttc cttcaagaac tgcaactgct gagtttgttg tacctaagtc aataccgata
    16261 attttagaca tgtgtttttc tccttgttag ttaattttct atttttcttt ttgatactct
    16321 tcttaagtgg cgacgccgtc agagcttgac ttcgtcaatc tctttgacta aactttgagc
    16381 ctaaggtctc aaaatttgcg caaatagcgc cacggcgaag agtatcgtct ttggttcgct
    16441 tcgctcacta ttacaaagcc aaacatattt tatctttctt catagtttat tgtcgtttcg
    16501 gacaagtttt ctattatgta aattgcgaca cgaggagtcg aaatcgattt tatttcgacg
    16561 acgagttagt aaggaagcta ggcaaacgcc atagcgattg ccgttttctg acgagttgct
    16621 ttagctaccg tcagaattgc cgaatcgaac acttcgtcaa tctcctattt ttactgcgag
    16681 ctttttacga gctttgtatc ttagttatac accactacca ttgctgggcg taggatgcgg
    16741 tcatggagtt tgtagccttt ttgaaagact tgggcgatgg tatctactgg gtgttcatcg
    16801 tctgctggga gagtttggat ggccatatgg tagttatggt caaattcgcc atctgctgcg
    16861 atttcttcaa ttccttcttc tttcaaagcg tgaatcaagc tttcttgcac catccccaag
    16921 cccttcttca catcatctgt caaaccttca actgcaagtg cacgctcaag gttgtcaaga
    16981 gatggtaaga ttgcttttgc caagtcctgg ctacgataac gttgcaagtt ttgacgttct
    17041 tcattggcac ggcgttggat attttgcatt tctgcatgag cgcgaagata tttgttttcg
    17101 aactcatctg cacgttcatt tgccaagtcc aactcagact tttcaggagt tgtttcttca
    17161 gctgtttcca caacttcctc ttcttgaact tcttctactt cttcattttt tatatcttgg
    17221 gccatgttcg cctcctttaa tgatttcaat cttaatgtac ttcgtaatga ttactgctga
    17281 ggtagcggta aaaatctgtc aacttcatgg tcaaaacacg gttgaccaca ttgacttgat
    17341 tgattagctg ttggtaatcc agattaactg gaccgataat ggctagaatt ccaactcccc
    17401 gataaggaat gaggaactta ctactgatta ccgctaggtc agctaaacag gactcttgac
    17461 cgtctgcaac acgaacattt tgcatctgat cctcacgcaa cccctcacga atctccaagg
    17521 ccaccttttg cggttggtca aagaactgat aggctgctag attggcaaaa ttcaagagat
    17581 ggaccttgcc cgccatcaca atgttttcgt tgaacatttc cttaaagatg tgttcaaaga
    17641 gatcgatgac attatccgtt gttgtaaagt aacgctggat aatctgcgga atctccgtcc
    17701 gaatcttgta gtgaatatct aaaacggtgt gaccgaggaa acgttcctga atgatgctct
    17761 tcagtttcag caaatcctcc tgcaagaagt tccttggaat cagaaactga ctagtaaccg
    17821 ttcgcgactc gtctagggta aataccgcca aggctgtatg ttgccccaaa acaacgatat
    17881 caaaggctgt caaacgttgc ctgctcggct caacatccag tgccactacc gtacagccac
    17941 tcaggtctgt tagtaagtta gcagcctctt gcagaatatc ctccaatttg aagaattcct
    18001 gatcaaaggc tttgacaatc tcatatacct cattttcagc cagccggtca aaatccagtg
    18061 agtgtttcac atagtactga aaaccagcaa cacttggcat ccgaccgctt gaagtatgag
    18121 ccttctcaag caacccttgt ttttctagtt ccgccatgtc attacgaatg gttgcactgc
    18181 tagagttaat agactcttgc aaggcttttg atccgacagg ttcgtgcgtt ttggtaaaga
    18241 tgtcaataat cagatttaaa atatcctgct gacgctctgt aaccatctca tcactcctct
    18301 ctgtctgatc aattagcact caatgcatcc aagtgctaac ttatgattct attatacacc
    18361 cttagaagag aatgtcaaga caaaaactca aaaaattagc actcttttat caagagtgct
    18421 aaaaatcaat ctgaagacct agaaccttgc tattcatcta ctcggtattt cctttttagt
    18481 ctgaaataac catgaattag ataagaaatc agaaaagcat ataaaacaaa aataagaaag
    18541 aaagacatag aaagatcctc tctaaacaaa ctcgtacaaa caacaagacc acctaagaaa
    18601 gtagttgtac aggaacgaag tctagatcgc ataacttccc atttgagccc cttactcata
    18661 tagacttgat cctctggaag taaatcagaa gacgatttac gaagaatcga acaagaacct
    18721 gctcctatca attcccaacc tctatctcta aaatcttgca gttcacgctt atgctcttta
    18781 agaaatctag aatcatagat acgatagata acatcgtccg gttgaccttg ctcaaaatag
    18841 aacaaaccaa aacgactagt tctatacctc caacctttca aatgcatctc atgtaaatat
    18901 tcttctttct tgtccaaatc aacaatggtg aaaatccgaa attctactct gctattcatt
    18961 gtcttacccc aaaattagaa aacatgccta gcgttattta ttagataatt ctttccactt
    19021 ttgactcaat ctccaaaaaa tataagaaat ctgaatcgca aaaactatca ataaaaccca
    19081 atctattatg aaaatcaaaa acacttccca actgaaagaa ctacctccag tgacaaactt
    19141 tgagaaaaac ggtagtagag ctaaaaagag aagcaaaata ggaagcatcc gcattgttaa
    19201 aatccttttg gcataaaaaa atctttattt aaacgaaaat attatggcaa aatttacgcc
    19261 agtttttgaa cggctgatgt agatatttta tactttcaaa atgtttaaat gtgattattt
    19321 atttttgaaa aatagctcac cagcccgact gaaagtgctt atagaatgat aataagtcgc
    19381 ctgccgaaaa cagcgaaaaa tagcggtgtt atgcggagat aatctgacgc gatgcgaaag
    19441 tatattgcat acttattttc aacaatttag cagagtattt ttataagtgt gatataatag
    19501 aagtataatt tgttctgata gtttatttta tggagaagta gatttttaga atgcggagga
    19561 ttcaatatgg ttgagtttat aaagtctaag aaagaaatga gtgaggagga tattaaagca
    19621 aatttcatca ctcctgctat tgtatccaaa ggatggaaaa atggtgagca tatcgcttac
    19681 gaagaatact tcactgatgg tcgaattgaa gttagaggag ataaggctcg tcgtaaagaa
    19741 ggaaaaaaat cagactattc actgtattac caatttggaa ctcgaattgc aattgttgag
    19801 gcaaaggata ataaacacag cgttcgagca ggattacaac aagctattga atatggagag
    19861 attttagatg ttccatttgt ttattcttcg aatggtgatg gctttattga acacgaccgt
    19921 atcacgagag aagaacgtga gctggagtta gacgaattcc ctactcgtga agaattattt
    19981 tctcgtatga cgaaggaaaa aggattgacg tacgaaatta cagaagctat ctcaactcca
    20041 tactatacag acgccttctc aatgaaaacg ccacgctatt atcagcaaat agctatcaac
    20101 cgtactattg aaacagttgc cagaggacaa aaacgagtaa tgtttgtgat ggcaacagga
    20161 acggggaaaa cgttcatggc ttttcaaatt attcatcgcc ttcgaaaagc tggtttggct
    20221 aaacgagttt tattcttagc ggatagaaac atcttagtag accaaacgat ggctgaagac
    20281 tttaggccat tcgaaaaggt aatgacgaaa attacaccaa aacttttgac tgctcctgaa
    20341 aaattaaatt cttttgaaat ttatctaggg ctttatcagc aactaactgg tgaagatgga
    20401 actgaaacac attatcaaaa atttgacaaa gacttctttg atttaatcgt aattgatgaa
    20461 gcgcaccgtg gttcagctaa ggaaaacagt aactggcgta aggtaattga ttatttcagt
    20521 tctgcgacac agattgggat gaccgctact cctaaagaaa ccaagaatgc ttccaatacg
    20581 gaatactttg gtgagccaat ctatacttat agtttaaaac agggaatcga ggatggtttt
    20641 ttggctccat atcgtgttat gagggttaat ttagatgtgg atgtggatgg ttatcgtcca
    20701 gaaactggaa aagttgatgc taacggacaa ttaatagaag ataggtacta cggcaggaaa
    20761 gattttgata aaaccattgt cattgatgat agaacgcaaa gagttgccaa gtttgtttct
    20821 gattatatga agcaaaacaa tgcacgattt gataaaacaa ttgttttttg tgttgatatt
    20881 gaccatgccg agcgaatgcg tgctgcactt gtaaaagaga atctagactt agtccaagaa
    20941 gactatcgtt atgtcatgca agtaactggt gacaacgctg aaggaaaagc tcaactggat
    21001 aactttatgg atgtcaattc taattttccc gctattgtaa caacgtctaa attattaacg
    21061 acaggagtta atgctaaaac atgtcgtttg attgttttag actctaatat ccaatccatg
    21121 actgaattta aacaaattat tggtcgtggc acacgtcttt atcctcaaaa ggggaaagaa
    21181 ttttttacga ttattgattt tcgaaatgtt accaatttgt ttgctgaccc tgattttgat
    21241 ggtgatccag tgaaggtgct ggaaacaggt gcgaaaacag tcagtggttc tacgcccggt
    21301 ttcgtagatg aggaaggtga cccagtagaa aaatatatcg ttacagacaa gcaggttacc
    21361 attcttaatt ctactgttca agtattggat gaaaacggga aactgattac cgaaagcctg
    21421 accgactaca ctcgaaagaa tatcttaggt agctacgcca ctttgaacga ttttatcaca
    21481 gtttggcata cggcagataa gaagaagctt atcttagacg aactttataa aaaaggagtt
    21541 tatctagatg ctattcgaga gtcggaggga atatcagaac aagaaatcga tgattttgat
    21601 ttactcctaa aacttgccta tggtcaaaaa gaattaacca aaacggaacg tatcaataaa
    21661 ctcaaacaaa gcggatattt atataaatat agtgaggaag cgcgtgctgt tttggaaatt
    21721 ttactgaaca aatacatgga taaaggtatt ggagaactcg aaagcattga aacattaaaa
    21781 cttccagaat ttcagatata tggtggaacc ttcaaaatca tcaatactta ttttggagat
    21841 aaaaaacgat atttacaagc aattaaagaa ttggagcaag agctatttac agtagcttaa
    21901 tgaaaggaaa gtatgtcaat tacatcattt gtaaaaagaa ttcaagatat cactcgaaac
    21961 gatgctggtg ttaatggtga tgctcaacgt attgagcaaa tgtcttggtt attattctta
    22021 aaaatttatg atagccgtga aatggtttgg gaattagaag aagacgagta tgagtcaatt
    22081 atcccagagg aattaaaatg gcgaaattgg gctcatgctc aaaatgggga acgggtattg
    22141 acaggcgatg aattacttga ttttgtcaat aacaagttat tcaaagagtt gaaagagctt
    22201 gaaataactt caattatgcc tattcgaaaa acgattgtta aatcagcttt tgaagatgcg
    22261 aacaactata tgaaaaatgg cgtcttgtta cgccaagtca tcaatgttat tgatgaagtt
    22321 gatttcaata gccctgaaga tcgtcattcg tttaatgata tttacgaaaa aattcttaaa
    22381 gatattcaaa atgctgggaa ctcaggagaa ttttatacgc cacgtgcagc gactgatttt
    22441 attgccgaag ttcttaaccc aaaacttgga gaatcaatgg cagaccttgc ttgcggaaca
    22501 ggaggcttct tgacttcgac tctgaaccgt ttaagtagtc aacgtaaaac tagtgaagat
    22561 accaaaaaat ataatacagc tgtttttggt attgaaaaga aagcatttcc tcatctttta
    22621 gcagttacaa atctgtttct tcacgaaatt gatgacccta aaattgttca tggaaatact
    22681 ttagagaaaa atgttcgtga atatacggat gatgaaaaat ttgacattat tatgatgaat
    22741 ccaccttttg gagggtcaga attagaaaca ataaaaaata actttccagc agaattacgg
    22801 agttctgaaa cagctgattt atttatggct gtcattatgt atcgtttgaa agaaaatggt
    22861 cgtgttggag ttattttacc tgatggtttt ctatttggtg aaggtgtaaa aactcgcttg
    22921 aaacaaaaac tggtagatga gttcaacttg catacgatta ttaggttgcc tcatagtgtc
    22981 tttgcaccgt atacaggaat ccatacgaac attcttttct ttgataaaac aaagaaaaca
    23041 gaagaaactt ggttttatcg tttagatatg ccagatggtt ataaaaattt ctcgaaaact
    23101 aagccgatga agtcagaaca cttcaatcct gttcgtgact ggtgggaaaa tcgtgaagag
    23161 attctggaag gtaagttcta caaatctaaa tcatttacac ctagtgaatt ggctgagttg
    23221 aattataatt tagaccagtg tggctttcca aaagaggaag aggaaatctt aaatcccttt
    23281 gagttgattc agaattatca agcggaaaga gcaactttaa atcataagat tgataatgta
    23341 ttagctgata ttttgcagtt gttggaggac aaataatgac accagaacaa cttaaagcaa
    23401 gtattctcca aagagcgatg gaagggaaat tagtgccgca aaatcccaat gacgaacctg
    23461 caagtgaatt attaaagaga attaaagctg aaaaagaaaa acttatcaat gaaggaaaaa
    23521 tcaaacgaga taaaaaggaa actgagatat ttcgtggtga tgatgggaaa cattatggga
    23581 agtttgctga tggaagtact caagaaattg atgttcctta tgatattcct gatacttggg
    23641 agtgggtgag gttttctaca ttggttgaaa ttatcagagg tggctctcca cgaccaatca
    23701 aagattatct tacttctgaa gtagatggaa taaattggat aaaaataggt gatactgaaa
    23761 agggtgaaaa gtatataaat aatgttaaag aaaaaatcaa aaaatcaggg cttaacaaaa
    23821 ctagatttgt aaaaaaaggt acatttttgt taactaattc tatgagtttt ggtagacctt
    23881 atattttgaa tgttgatggt gcaatacacg atggatggtt ggctatttcg aactatgaaa
    23941 actcattaaa taaagattac ctattctata ttctttcatc aaatgtagtt tattctcaat
    24001 ttctatctct aattagtgga gctgttgtga aaaacttgaa tagtgataaa gttgcttcta
    24061 ttcttatccc tctcccccca ctatccgaac aacaacgaat agtagaagca atcgaatcag
    24121 ctttagaaaa agtagatgaa tatgctgaaa gttataatag actagaacag ctagataaag
    24181 aatttccaga taaactaaaa aaatctattc ttcaatatgc tatgcaagga aaattagttg
    24241 aacaagaccc aaatgatgaa tcggtcgaag ttttacttga aaaaatacga gcagaaaaac
    24301 aaaaactctt tgaagaaggc aagattaaaa agaaagattt ggacatttct attgtttccc
    24361 aaggagatga taactcttat tatgggaaca aagacgaaac aacctcttat cctatatatg
    24421 aaatcccaga agcatggaga tatattaaat ttgctagcct agttaacttc agaataggaa
    24481 aaactcctcc acgcagtgaa gctacttttt ggggaactga aataccttgg gtatctatat
    24541 cagatatgcc tatatctggt tatgtaacta atacgagaga atctatttct aaattagcat
    24601 taaaatcaaa gaaaatagac atttcaccta aaggaacttt attaatgagt tttaaattat
    24661 ctattggaaa agttgccata ttggatattc ctgccactca taatgaagcc attatatcga
    24721 tattccccta tgctaataaa gaaaatatta tcagagatta cttaatgata tttttgccac
    24781 ttatctctac tttaggtgat tcaaaagatg ctatcaaagg gaaaacatta aatagtacca
    24841 gtatctccga attattaatc cctaatttct aatcatgaag agatgaaaag aatcatttcc
    24901 aaagttgatt tactttttca aaaagtttct caactttttg agtaataagt tcctgttcct
    24961 caaaaggagc taacggaatt aatagctcgc tcagtgtagt tttaggaata ttatataaag
    25021 cttgacctga tagtttagtt attgctttca attgtttata aaataacgga gaggacaagt
    25081 taaataatag aaattttgaa ataatctctg aactttcgaa tggtgttaat tggaaaataa
    25141 atccaccagc cacaacacca tcatagtctt tatcgattct tgcaaacttt ccaatatgtt
    25201 ctaaagaggt tgatacaggt gttattagct gattatgttt taaataaact tgctcagagg
    25261 agatgaattg tgtatcaatg tagtaatcgt tatccaacag agaaaattct aaaggcttaa
    25321 tattaccacc acgtataatt ctaacacctt tattattaat gcttaaatcg cccttcttgt
    25381 aagaaagacc tgtattcatt gaaaaaatat cttttatttt tataacaacc caattcatag
    25441 gtat
//
LOCUS       DAVHEX010000029        25391 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVHEX010000029 DAVHEX010000000
VERSION     DAVHEX010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25391
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.029"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(121..1755)
                     /locus_tag="VVL45_001164"
     CDS             complement(121..1755)
                     /locus_tag="VVL45_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187346.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNPGKKVNVSARV
                     LSSYIFSSLLMIICFSNIAIHFPFEEIPIYFSSMIAILLVIAFSTSLTAFYNVFYESK
                     DLISYRPYAFKESEIIIAKGLSVLLPALTGIVPILAYFLVLYIRLAPSLWLGLPLMLL
                     SLTLLFVSVALVMVVAVHFLAQTRVFRKYQSIFSNVMIGIGVLIPLIFIFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSTEALVGLLAWIGLTLFLLYLTKKKVLPRFYD
                     VILLNSEEKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVVTVLFTSAFLPYL
                     MMIGLISKIRDSQIVPDIHPPYWLPLFFIALFIAVVNNNITSLHSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIFAVQSFLPILTLLGLSLYLGLSILSMIYLLVVWIIASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLSP
                     ILAINLGVGALIVLLALAIFGYHYYLSRILAEIEKR"
     gene            complement(1736..2517)
                     /locus_tag="VVL45_001165"
                     /pseudo
     CDS             complement(1736..2517)
                     /locus_tag="VVL45_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            2695..4149
                     /locus_tag="VVL45_001166"
     CDS             2695..4149
                     /locus_tag="VVL45_001166"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEU4187347.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKAQGIQPMADVVL
                     NHKAAADHREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNGFHWHWYHFT
                     GTDYDAKRSKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNSDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQQDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGSHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            complement(4239..6857)
                     /gene="alaS"
                     /locus_tag="VVL45_001167"
     CDS             complement(4239..6857)
                     /gene="alaS"
                     /locus_tag="VVL45_001167"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEU4187348.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLISPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVAELKEKGQSVIAGQ
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNASQLSSKLVAIVADNAEVGAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKVVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVDNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAIAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDVKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQPKIQELLDAVAGKL"
     gene            complement(6879..7364)
                     /locus_tag="VVL45_001168"
     CDS             complement(6879..7364)
                     /locus_tag="VVL45_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEU4187349.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSQISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            complement(7639..7878)
                     /locus_tag="VVL45_001169"
     CDS             complement(7639..7878)
                     /locus_tag="VVL45_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187350.1"
                     /translation="MKMSTFFNKSFWPTFMIVNQTAILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            complement(8131..9201)
                     /locus_tag="VVL45_001170"
     CDS             complement(8131..9201)
                     /locus_tag="VVL45_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU4187351.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQNS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            complement(9198..9971)
                     /locus_tag="VVL45_001171"
     CDS             complement(9198..9971)
                     /locus_tag="VVL45_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4187352.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIITGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            complement(9968..10774)
                     /locus_tag="VVL45_001172"
     CDS             complement(9968..10774)
                     /locus_tag="VVL45_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4187353.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWIMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDEMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            complement(10755..11912)
                     /locus_tag="VVL45_001173"
     CDS             complement(10755..11912)
                     /locus_tag="VVL45_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4187354.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFEPEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            complement(12072..12977)
                     /gene="murB"
                     /locus_tag="VVL45_001174"
     CDS             complement(12072..12977)
                     /gene="murB"
                     /locus_tag="VVL45_001174"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEU4187355.1"
                     /translation="MSVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARVVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKNGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESK"
     gene            complement(13089..13841)
                     /locus_tag="VVL45_001175"
     CDS             complement(13089..13841)
                     /locus_tag="VVL45_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HEU4187356.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWLEKKR
                     SLEKADALAYGLGHGGLELIFLGLTSLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLAAYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALELVLVAYGTKEIFCKKS"
     gene            complement(13851..14582)
                     /gene="budA"
                     /locus_tag="VVL45_001176"
     CDS             complement(13851..14582)
                     /gene="budA"
                     /locus_tag="VVL45_001176"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEU4187357.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHRAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFLHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            14724..15995
                     /locus_tag="VVL45_001177"
     CDS             14724..15995
                     /locus_tag="VVL45_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEU4187358.1"
                     /translation="MKKQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEERVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYSLHDGLLLDLRQYPKIASLKHSQLGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(16015..16830)
                     /locus_tag="VVL45_001178"
     CDS             complement(16015..16830)
                     /locus_tag="VVL45_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU4187359.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVDAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKVQLTSLTNMGEAVNELQAGKIDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVVQKLKDEGTYQSYLEKAASLTEVEE"
     gene            complement(16987..17640)
                     /gene="phoU"
                     /locus_tag="VVL45_001179"
     CDS             complement(16987..17640)
                     /gene="phoU"
                     /locus_tag="VVL45_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU4187360.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(17652..18410)
                     /gene="pstB"
                     /locus_tag="VVL45_001180"
     CDS             complement(17652..18410)
                     /gene="pstB"
                     /locus_tag="VVL45_001180"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU4187361.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            complement(18423..19226)
                     /gene="pstB"
                     /locus_tag="VVL45_001181"
     CDS             complement(18423..19226)
                     /gene="pstB"
                     /locus_tag="VVL45_001181"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU4187362.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(19237..20121)
                     /gene="pstA"
                     /locus_tag="VVL45_001182"
     CDS             complement(19237..20121)
                     /gene="pstA"
                     /locus_tag="VVL45_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU4187363.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            complement(20111..21028)
                     /gene="pstC"
                     /locus_tag="VVL45_001183"
     CDS             complement(20111..21028)
                     /gene="pstC"
                     /locus_tag="VVL45_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU4187364.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(21028..21906)
                     /gene="pstS"
                     /locus_tag="VVL45_001184"
     CDS             complement(21028..21906)
                     /gene="pstS"
                     /locus_tag="VVL45_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEU4187365.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            complement(22480..23784)
                     /locus_tag="VVL45_001185"
     CDS             complement(22480..23784)
                     /locus_tag="VVL45_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEU4187366.1"
                     /translation="MKFSEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQIFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEAFVKYAAGET
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            complement(23785..24558)
                     /locus_tag="VVL45_001186"
     CDS             complement(23785..24558)
                     /locus_tag="VVL45_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEU4187367.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            complement(24548..24826)
                     /locus_tag="VVL45_001187"
     CDS             complement(24548..24826)
                     /locus_tag="VVL45_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEU4187368.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            complement(24826..25227)
                     /locus_tag="VVL45_001188"
     CDS             complement(24826..25227)
                     /locus_tag="VVL45_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEU4187369.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
BASE COUNT         7607 a         5811 c         4900 g         7073 t
ORIGIN      
        1 cttattcaaa acattcctcc ctatattttt gattaccaat cttaatcatt tacaactaca
       61 ttctaacaaa ctataaaagc gtttgtcgaa ttgaatttat caagcaagca accaactatc
      121 tcatcttttt tctatttctg ccaatatgcg tgacaggtaa taatgatagc caaaaatagc
      181 aagagcaagc aagacgataa gagctcctac tcccaagttg atggcaagga taggggagag
      241 agactgaacc aagaatatgc tcccaattac aagggccatc aggattgcac tataaataaa
      301 caataaaact atggcgacta tgccatttga acgattcacc aggtccgtaa tgctactcca
      361 attggttgac agatttttaa cgtccttaaa gtaatggtgg caagaaagga tgacactggc
      421 aatgatccag actacaagaa ggtaaatcat cgaaaggatg gacaagccta gatatagaga
      481 aagaccgagc aaagtcagaa ttggtaaaaa ggactgaaca gcaaagataa tccaaaattt
      541 cactttcaca taacgagcaa agtcaaaggg taaactctta agaaaatcaa cattttccct
      601 ctccaaggac aaggcaattg aatgcaggct ggtgatattg ttattgacaa ctgctataaa
      661 gagagctata aaaaacaagg gtaaccagta tggaggatga atgtctggaa ctatctgaga
      721 atctcggatt ttggaaatca gaccgatcat catgagataa ggaaggaaag cacttgtaaa
      781 aagcactgta accacgccag taccctgtcc caagagggtg aggtggtagc gtaaaaccat
      841 gcggaaaaat ccctttttag tggttgaaat tctctccttg ctgcgacgtt cttttttgac
      901 cttctcctca ctattaagca ggatcacgtc ataaaaacga ggaaggacct tctttttggt
      961 cagataaagc aggaagagag ttagtcctat ccaagcgagc agacccacta aggcttctgt
     1021 cgaaaaaggc tccactgcta ttttgtaaaa gatatgaaga ggataaagga gaaatggaat
     1081 gtctctaact ttgtcaacaa tacttccaaa agtcgactga agaaagaaga taaatattaa
     1141 aggtatgaga actcctatcc caatcatcac attcgaaaaa atagactgat actttctaaa
     1201 gaccctagtc tgagccaaga aatgcactgc cactaccatc actagagcca cagagacaaa
     1261 taataaggtc aaggacagta gcatcaaagg caaacccagc catagagaag gagctagcct
     1321 aatgtagagg actagaaaat aagctaggat tggtacaatt ccagttagag ctggcaaaag
     1381 gacagacagt cctttagcaa ttataatctc tgattcttta aaggcatagg gcctatacga
     1441 tatcaaatcc ttactctcat aaaagacatt gtaaaaggcc gttaaagaag ttgaaaaggc
     1501 aatcactagt aaaatagcaa tcatcgagct aaaataaata ggtatttcct caaaaggaaa
     1561 atgaatggct atattactaa aacagatgat catcaagaga ctggaaaaaa tgtaagaact
     1621 taagactcta gcggaaacat ttactttttt cccaggattc ttagcctgct tctttcgtag
     1681 attagccaga ttagcttctt gagatgaata aaggatattg atatcaacta attttttaat
     1741 gaccttgaga cgcatccgca acctcctctt ttctaccagc aagactaagg tagatacttt
     1801 ccaaagactg gtctgggtaa tcttttctca agtcctctac actaccacaa taaatcaaat
     1861 gccccttttt caaaatggca atccgatcac agacttgctc tgccacctct aggacatgag
     1921 ttgaaaacaa gactgtcttc cctttttgtg catgttcctt catcatctgt ttcaaatcaa
     1981 aggcagcctg gggatccaaa ccagtcaagg gttcgtccaa gacccaaata tcaggatcag
     2041 acaagagtgc cccgatgaca aaaactttct gacgcattcc atgagaaagg gtttcaataa
     2101 cctgataacg attttcagca aaatcaaaaa cgttcaatag cctagctaga ctagctctga
     2161 cttggagact agcctagcta gactagtctc caagtcagag ctagtcagat cataggatga
     2221 ggcgatcaat tcccaaaatt cattggccgt taaacgtaaa aataagtcag gcgagtctgc
     2281 tacgtagcca atctttcgtt tgatcgccaa gcgattttcc gataactcct gaccatctac
     2341 caaaatacga ccactgctgg gtgaaatgat actgactagg gattttatgg tggtcgattt
     2401 tccagcccca ttatgaccaa tcaagcccat aatctctcca ttttcaatct gcaaattgag
     2461 attactcaag gcctctttat caccatacaa cttactaaca ttttgaaatt caatcatggg
     2521 atgactccta tctttatcta tattacatac tattatacta gcactttgat acaaaaaatc
     2581 aatactttat tttaagcagt taaaatagtt tccttctatc ttacgcaaac gtttgcgcta
     2641 atagatactt tatgatagaa taaagaacaa gattgacaag taagaggaaa cattatgcaa
     2701 aatcaaacac tcatgcaata ctttgaatgg tatctgcccc acgacggtca acactggacg
     2761 cgtctggctg aaaatgctcc acacctagct catctgggga tcagtcacgt ctggatgcca
     2821 ccagccttca aggcaaccaa cgaaaaagat gtcggctatg gggtctatga cttatttgac
     2881 ttaggagagt tcaaccaaaa agggactgtc cgcaccaagt atggtttcaa agaagactat
     2941 cttcaagcca ttcaagccct taaagcacag ggaattcaac ctatggccga tgtagttctc
     3001 aaccacaagg ctgctgccga tcacagggaa gcctttcagg ttatcgaagt tgatcctgta
     3061 gaccgtacag ttgaacttgg agaacccttc accatcaatg gctggactag ttttaccttc
     3121 gatggtcgcc aagataccta taatggcttc cactggcatt ggtaccactt caccggtaca
     3181 gactacgatg ccaaacgcag taaatctggg atttatctga tccaagggga caacaagggc
     3241 tgggctaacg aggaattggt cgataacgaa aacggaaact acgactacct catgtatgcc
     3301 gacctagact ttaaacatcc tgaagtcatc caaaacatct atgactgggc tgattggttc
     3361 atggaaacga ctggtgtagc tggtttccgt ttggatgccg ttaagcatat tgactctttc
     3421 tttatgcgca acttcatccg cgatatgaag gaaaaatacg gtgacgattt ctatgttttt
     3481 ggtgaatttt ggaactcaga caaggaagcc aatctggact atctcgaaaa aacggaagaa
     3541 cactttgacc ttgtcgatgt tcgtctccac cagaatctct ttgaagccag tcaagctggc
     3601 gcaaactatg accttcgtgg cattttcaca gatagcctgg ttgaactcaa gcctgacaag
     3661 gctgtgactt ttgtcgacaa ccacgatacc caacgaggac aagcccttga gtctaccgtt
     3721 gaagaatggt tcaagccagc agcctatgcc ctcattttgt tacgccaaga cggccttcca
     3781 tgtgtctttt acggagacta ctatgggatt tcagggcagt atgctcaaca agatttcaaa
     3841 gaaatccttg accgcctcct agccatccga aaagacttgg cctatggaga acaaaatgac
     3901 tactttgacc atgctaactg tatcggttgg gtacgttcag gtgctgaaaa tcaatcccca
     3961 atcgcagtcc ttatctcaaa tgaccaagaa aacagcaagt caatgtttgt cggtcaagaa
     4021 tggactaatc aaacctttgt agatttactt ggtagccacc aaggtcaagt tacaattgat
     4081 gaggaaggtt atggacaatt ccctgtctca gctagatccg taagtgtctg ggcagtcaat
     4141 accatctaat agctcataat aaccaagcta ggtccaagcg gatttggctt ttttgtattc
     4201 acaaaaagac ctacccaaat ggatagatct ttacttgatt acaatttacc tgctactgca
     4261 tccaacaatt cttggatctt aggttggttg cttcctcctg ccatggccat atctggttta
     4321 ccaccaccac gtccatcgat gattggtgct aattctttga caaggtttcc tgcatgaagg
     4381 tcttttgtct tgcttgctac aaggacattg actttgtcac cgatagcggc aactaggaca
     4441 agaagatcag agtagtcttt ttgtttccag ttatctgcaa aagtacgaag ggcaccggca
     4501 tcggatacag acacttgact agcaatgtaa cgatgaccgt tgacttcctt aacatctttg
     4561 aagatatcgc ctgcggctgc agctgcggct ttttctttca actcagcatt ttctttttga
     4621 agttgacgaa gttgttcttg aagtccttct accttgtgag gtacttcctt gacttgaggt
     4681 gctttcaagg ttgctgcgat agctttaaga gcatcctctt gttcacgata ggcttcaaag
     4741 gcttccttac cagtcactgc caagatacgg cgagttcctg aaccgattcc ttcttctttg
     4801 acaattttga agagaccaat ctcagaagtg ttgtcaacat gagtaccacc acaaagttca
     4861 atagagtagt caccgatagt cacgacacga acttccttgc cgtatttctc accaaagagg
     4921 gccatagctc ccatttcttt agcagtgtca atatccgttt caactgtctt cacttcaagt
     4981 gcttcccaaa ttttctcgtt aacttgctgt tcaatcgcac gaagttcctc agcagttact
     5041 gcttggaagt gggtaaagtc aaagcgaagg aattcaactt cgttaagaga tcctgcctgt
     5101 gttgcgtggt ttccaaggat attgtgaagg gcagcgtgaa gcaaatgagt cgcagtgtgg
     5161 tttttcatga cacggtgacg gcgattgcta tcaattgcca aggtatattc ttggttcaag
     5221 gcaagcggtg caaggacttc aactgtatga agggcttgac catttggggc tttctgaaca
     5281 ttggtcacag tagccacaac cttacctgac tcatccaaga tttgtccgta gtcagctacc
     5341 tgtccaccca tttcagcata aaatgacgtt tccgcaaaga taagagaggc agttccttct
     5401 gaaacagctc ctacttctgc attgtcagca acgatagcca ccaatttaga agacaattgg
     5461 ctagcattgt agttgaagac actttctaca gtgatgtttt gaagagtttc attttgcata
     5521 cccattgagc cacccttgac agctgacgca cgcgcgcgtt cttgttgttc tttcatggct
     5581 gcttcaaaac cttcacggtc tacagtcata ccagcctctt cagcaatttc ttcagtcaat
     5641 tcaactggga atccgtatgt atcatagagt ttaaagacat cttgaccagc aatgacagat
     5701 tgaccttttt ctttcaactc tgccacaatg ccttgggcaa agtgttgacc tgagtgaagg
     5761 gtacgggcaa atgattcttc ttcgctctta acgattttct caataaagtc acgtttctca
     5821 agcacttctg ggtagtagct ttccatgatt tttccaacag ttggaacaag tttgtaaagg
     5881 aaaggctcgt tgatacccaa tttttgacca tgcatagaag cacgacggag aagacgacga
     5941 agaacataac cacgaccttc atttcctgga agggcaccat caccgatagc aaatgaaaga
     6001 gaacgaatgt ggtctgcgat aaccttgaag ctcatgttgt cgccatcttg gtcataaacc
     6061 ttaccagaca atttctcgac ttcacggata atcggcatga agaggtccgt ttcaaagttg
     6121 gtcttagccc cttggataac agccaccaaa cgctccaaac cagcgcccgt atcaatgttc
     6181 ttatgtggca attccttgta ttcgctacga ggaacagcag ggtctgcgtt aaattgtgac
     6241 aaaacaatgt tccagatttc aatataacgg tcgttttcaa tatcttctgc aagcaggcga
     6301 agaccgatat tttctgggtc aaaggcttcc ccacggtcaa agaagatttc tgtatctggt
     6361 ccagaaggtc ccgcaccgat ttcccagaag ttgtcctcaa ttggaatcaa gtgacttgga
     6421 tccactccca cttcaatcca gcggttgtaa gaatctttat cgtctggata gtaggtcatg
     6481 taaagttttt cagcagggaa atcaaaccat tcagggctta tcaaaagctc ataagcccaa
     6541 gtgatagctt cgtcacggaa gtaatccccg atagagaagt tccccaacat ttcaaacatg
     6601 gtatggtgac gcgcagtctt ccctacgttt tcgatgtcgt tggtacggat agccttttgg
     6661 gcattggtaa tacgtggatt ttcagggata atggtcccgt caaagtattt cttaagggtt
     6721 gctaccccag agttgatcca caaaagagtt gggtcattta caggaaccaa acttactgat
     6781 ggttctactg agtgaccttt ggtcgcccag aaatcaagcc acatttggcg tacttgtgca
     6841 ctagatagtt gtttcatatt gtctccttat ttacttgttt aatgtgattg gctttccagc
     6901 atttccacat agtcaatcgc gacacagagg gaaatgacta ggtctgcata agcgtcttca
     6961 agaaccgtta cggtataggt agaggtcaga tggaagagtt ccttcttaat ttccgcaatc
     7021 aactgatcgc gatcatccag caatttgaaa ttcaaatccc agatattgcc ctcgatacga
     7081 agacctagat tatcaaactc atacttatct cgccaaaagg tcaacttctt acgaatgaca
     7141 aaactcgagc catcccgaag ctgaatctca aaacgaggaa gcaaggtcaa gatttcttta
     7201 ctgatctgac tgacttgttc accagccgca tcatagatgg taaaagtttt gggaatctta
     7261 aaaaatgatc cctccacctg ataggcaatt tctcccctgt catccttgat agcgaagcgt
     7321 tcgcctccaa gacgaaactt ttgtttgaca agaaatgttt tcatcaacac ctccaaaaat
     7381 caaaagacaa gctcatatca cgaagggcga aaaaccgcgg taccaccttc attcaatgaa
     7441 cttgtcattc tcttgttctt atgcaattgt atgattgagt agcatgactt cctagcttag
     7501 atggctcgca gcaccgccat ttctctggac taagacaagt gaaaatcaat tctcaacttt
     7561 cttattatat cgttttttta atcttgcgtc aactggaaat gatctccgtt gaattagacc
     7621 aattccctac atctctgatt actttttcag gatatatttt ttcttactgc cgtttttctt
     7681 tttatcccaa attttcatat tactaaacac agctactaga atatttccaa atataaaggt
     7741 gcctatcacc caatatatgg actcagttgt taggtattgt cgatccaagc catcctttaa
     7801 atggaatagt atagcagttt ggttaacaat cataaaggtt ggccagaaac ttttgttgaa
     7861 aaaagtagac attttcatta tttgttgccg ctttctgtaa ggttaatact caataaaaat
     7921 caaaaagcaa actaggaagc tagcctcaag ctgtacttga gtacggcaag gcaacgctga
     7981 cgtggtttga agagtatagg cttagtatac tactaggcaa gcaaataagc aaataaacaa
     8041 ctagaataga aaaagatagg gctctaaaaa ctgacttcta ttccttaaaa acgaaccagc
     8101 ttgactgatt cgtcttctta cgtttatctc ctacttccga tacattttaa actgtaggaa
     8161 gaggtcgcta tatttccctg tccatttatg gtcaaatttc tcataaactt ctaggtgttt
     8221 catggtttca acatcgggat agaaggcctt atcttccttt gtttcctctg ggagcaattc
     8281 cttcgctggt aggtttggtg ttgaatagcc gacatactcc gcattttgga gagcattttc
     8341 aggtttcaac ataaagttga taaaggcata ggctgagttt tggtttttaa ctgttttggg
     8401 aatgaccata ttgtcaaacc aaagattgct ggcctctgtc ggtaccacat aacgtagatt
     8461 ttcatttttt tctaacattt ggctggcttc accagagaag gtcacgccga ttgcaacatt
     8521 attctgaatc atatagccct tcatctcgtc cgcaacgata gccttgatat ttggagtcag
     8581 tttgtagagc ttatccactg tctcttccaa ctgctgcaga tccttggagt tgaggctgta
     8641 gccgagggaa ttgagtccta gtcccagcac ctcacgcgcc ccatcaaaga gcatgataga
     8701 attcttatac tccggcttcc aaaggtcatc ccaatgctca ggcgcttcat ctaccatggt
     8761 ttcgttgtag acaattccta aggttcccca gaagtaaggg atggagaatt tattacctgg
     8821 gtcaaaggac tggttgagaa actctggtcc gatattttcg attccttcaa tttttgaata
     8881 atcaagcgga accaagaggt cttcgtcctt catcttgtta atcatgtatt cacttggaat
     8941 ggcaatatcg taggtcgttc caccctgctt tatcttagtg tacatggctt cgttggagtc
     9001 aaaagtctcg tactgaactt gaattcctgt ttcttctgta aactgagtca agagttcagg
     9061 atcgatatag tctccccagt tatagataac caatttttga ctatctcgac tattgatttt
     9121 actatctaaa tgagtcgcaa ttccccacaa gacaaggata atcgctgcaa ttcctgctaa
     9181 aaatgaatag atttttttca tgcttgctcc tccttctcac gagagataaa gtaataacct
     9241 acaactagga taatactaaa gagaaagact agagcagaca gggcattgat ttctaaggaa
     9301 atccccttgc gagcacgaga gtaaatctcg actgataggg ttgaaaagcc atttcctgtt
     9361 acaaagaagg tcacggcaaa gtcatctaac gaataggtga aggccatgaa ataaccagta
     9421 atgatagacg gagtcaggta aggaagcatg atttccttga acatctgaaa ttgactagct
     9481 cccaagtcat aggccgcatg aatcatgtcg ccattcattt ccttgagtcg aggcaagacc
     9541 atcaagacca cgataggaat ggagaaggcc acgtgactag atagaacggt caaaaagcca
     9601 agtgaaaact tgagttgggt aaagagaatc aagaagctag caccaatcat aacgtcaggc
     9661 gcaaccatga ggatattatt gagtgataga aaggcttctt ggtatttctt acgagactgg
     9721 tagatgtaaa tggcaccaaa agtcccgata atggtcgcta tcaaggctga taggaaggcc
     9781 aagaaaaatg tctgagccaa aatcagcatg agtctcccat ctccaaacat ggtttcaaag
     9841 tgagtccagc taaaacctgt aaagctattc atatcatcac cagcattaaa ggcatagcca
     9901 atcaagtaaa aaatcggcag gtagaggacc agaaagacca gtcccagata aaggttggca
     9961 aattttttca tcgttctctc ctttccttag tcacccacat ggtgatgaac atggtcagga
    10021 tgagaatcac accgatagtt gaacccatac catagttgtc attggttaga aaattctgct
    10081 caatagccgt ccccaaggtg ataacgcggt tcccaccaat caaacgggtc agcatgaaga
    10141 gactcaaact tgggataaag accgactgaa ccccacttcg cacaccgttc atagatagag
    10201 ggaagatgac atgacggaag gtctcccact tagttgcacc aaggtcgtaa ctagcattga
    10261 tgagattatt atccatttcg tctaagacat tgaaaatcgg caaaatcata aaggggagct
    10321 cgatgtagct tgcgacaaag ataaatgaga aatcagtaaa tagcaactgc tgtgaaccaa
    10381 ttccgataaa ttccaagaat tggttaatag agccattctg accaaaaatc ccgataaagg
    10441 cataagcctt aagaagaagg ttgatccaag ttggcagaat aatcagcata atccagagtt
    10501 gacggtgttt gagacgggtc aaaaagaggg ccgtcggata actgataagc agtgtcacaa
    10561 aggtcacaat gcctgcataa agcactgagt tgaaactcat tttaagatag gtcaagtttt
    10621 gtgacgcaaa gtaagatttg taattttcta aactgaactg gccttcgatg ttgaaaaagg
    10681 attgaccgaa aatcaagacc aagggtgcca atacaaagag cgcaatccaa agcatgtagg
    10741 gtactacaaa gagtttagag cttgttttct tcatctcttt cctcctcgat tgcattgatc
    10801 aaacctgctt cttgctcttc gatttctacg tactcctcaa tacgagcatc gaactcttct
    10861 tcggtttcat tgagacgcat gatgtggatg tcttctggtt caaagtccag accgatttcc
    10921 tcacccacga tagccttacg ggttgagtgg atcatccatt catttccaag ttcgtcatag
    10981 gcgataattt cataatgaac tccacggaag agctgggtgt cgaccttaac ttggagtttg
    11041 ccttcttcag gaagggtaat gcgcaagtcc tctggacgaa taacgacctc aacaggttca
    11101 tttggcttca tcccaccatc aaccgcttca aagcgtttgc cgttaaattc gaccaagtag
    11161 tcctcaatca tggtacctgg caagatgttt gactccccga taaaggtggc aacaaagtgg
    11221 ttgattggct catcgtagat gtccacaggg gttccagact ggacaatctc gccatcattc
    11281 ataacgaaaa tccagtcact catggcaaga gcttcttcct gatcgtgagt gacaaagaca
    11341 aaggtaatgc ccaatcgttg ttgtaattca cgcaattcgt actgcatgtc tgttctcaat
    11401 ttcaagtcca gcgctgataa aggctcgtcc aacaagacca cacggggttg gttgatgata
    11461 gcacgggcga tggccacacg ctgacgttgt cctccagaaa gtttgcggat ggaacgtttt
    11521 tcataacctt ccaactgaac catcttgaga acttccgcta cacgctgctc gatttctttc
    11581 ttatcaattt tacgcaagcg aagtggaaag gcaacatttt caaacacatt catatgtggg
    11641 aacaaggcat aggattggaa gacggtatgt acgtcgcgct tgttggttgg aatatcattg
    11701 atacgaacac cgtctagcat gatatctcct gtcgtcgcat ccagtaaacc tgcaataatg
    11761 tttaggatag ttgatttccc cgaaccagat gcacctagaa gggtgtagaa tttcccttct
    11821 tccaactcaa agttgatgtc tttgagaacc ttggtgttgc tgtcttcaaa aactttagag
    11881 acgtttttga attcgataat tggttttttc aattggcata aattccttct ttttcataga
    11941 ttaacagatc ggggctctgt caggtcgcca ctacctcttg cagggagtaa aaccacctgc
    12001 atacatcttc gctaccaata ggctttcacc ccctttccat gacatagcag cagcttttca
    12061 aatacagctt cctacttgct ttcacccaag atccggactt ctctttcaag cgtaatacct
    12121 gagtgttcct tgactttttc gataaccgat tggatcaagt cctcgtagtc tttggccgtt
    12181 ccatctgcga cattgatcat aaatcctgca tgcttttctg acacttctac gccaccgata
    12241 cgatagcctt tcaagccagc ttctgaaatt aactgacctg caaaatgccc gactggacgc
    12301 ttaaagaccg aaccacaaga tgggtattcc aaaggttgct tgagttcacg taggtgcgtc
    12361 aagcggtcca tttcctgctt gataacctga tgggttcctg gagctagggc aaatttaact
    12421 gacaagacaa ctgcaccaga ctcctgaata gctgaatggc ggtaaccaaa agccaagtct
    12481 ttagcagaca gggtttcgat ttctccattc ttggtcaaga ccttacaaga ctgcaagata
    12541 tgagcaatct cgccaccata ggcacccgca ttcataaaga cagcaccgcc aacgcttcct
    12601 ggaataccac aagcaaactc aaagccagtt aaactatgac ggagggcaat gcgagttgtt
    12661 tcaatcaagt tagccccagc ttctgcttca atggtatagc catcaacaga aacgttattg
    12721 agcttgtcac acaagatgac aaatccacga atcccaccat cacgaacgat gatattgctt
    12781 gcattgccaa gaaccatcca agggatattt tcttggttgg caaatttcac aacgcgagcc
    12841 aactcaaaac gatttcgtgg aaagaccaaa taatcagcct ctccacctac ttttgtataa
    12901 ctatagctat gcaagggttc cttaaaacgg atatcaattc cttctaagat ttcaagcatt
    12961 ttttctctta cagacatgtc actcttcctt ttacaaaatt cattccatta taccattttt
    13021 agagacattt gacgaccata aaaatacctt gtttggattt tgcataagaa aaagaggttc
    13081 cccccttttt atgatttttt acaaaagatt tccttggttc cataggcgac cagaacgagc
    13141 tccagtgcta gaatcacttc aaccaagact ggatttgtca accagcctac ttggaaaaga
    13201 gatggtgcca gatcaaagaa ggcatgcaag ccataggctg ctaggagata aatccatttc
    13261 ttctggcgaa cagcttggta aacccaaact gtcaaaagta attggaaacc aagcgccaag
    13321 attcgctcaa aaccaagcaa ataaatctgc cagaccgaaa gtgactgaat ggtttttaac
    13381 atattttcag acagtaattg cataacctgt ggattctgag tttgaactgc cgaaagaacg
    13441 atgtaaagat tgagtaaact agtaaggcct agaaaaatca actccaagcc accatgccct
    13501 aggccatagg ccaaggcatc tgccttttcc aaacttctct ttttctccaa ccatttgaag
    13561 aaaacaagac gagccgtttc ctcaaaaaat gctgccatgg ctaggccata gataatatag
    13621 ataagcggat gatcttgcaa gagggcaata ctaccgtctt tttgagggtg caaaatcaag
    13681 atatgcacca gtttttccaa aatctgcgaa gagacaaaga aagcgactgc acccaagccc
    13741 aagacagcta gattaatctg gtatttcttt ttggcaaacc aaatgcttcc tatcagaaaa
    13801 gctagcaaca gcaacatggt aataatgata tgaatggtca ttttcttctc ctattctgcc
    13861 ttttcaatat cttttttcat ctcgtcaaca ttgaacttag caaacaagta ttgacggtct
    13921 tggactggga aacgttggtc caactggtca actgctccca cctcgataat gccttccttg
    13981 atgacaaaat ccatgacatg tccaccgaag gtcaaatcat ctgatatgaa gtgcagatgg
    14041 tagcctgcca cactgacccc atggaaaatc tcaggcgtcc agaaaccaac aatagtcccc
    14101 gcaacattgt cacgactata ttccggttga tgggttgcga catcagcaaa cttggtatcg
    14161 ggtgttgact taggaatcat acgcacatgc atatgtaaaa attccccccg aatcttgata
    14221 gagcggaaaa gattttcccc atcataataa gactcaattc gttctttcaa ttccttgtct
    14281 gtcatctcaa agcgctggcg gaaaatgacc tctgcccgat gcggtaccac tgcagcgtaa
    14341 ggaataaggg catctggtga cacttccaca atttctggct ggtctcctga ccctttggcc
    14401 tgataagcct tgccatccaa gacaatcaat tccccatcaa tggaatccaa ggttcccaaa
    14461 ccaaggtcac catgttctag caattctcct actgtcatag taccaccata aaggccagcc
    14521 atcaaggctc caagagtatt gtattgaaat aatttcactg gttcctgcac gttcttatcc
    14581 attcactttc ttgtctgtta ttctataaat cttactccta agtataccac atttgcccct
    14641 agatgtgaac gagagaaacg ctctagacat tgccaagaag gaaaaaaaag ggtacaatgt
    14701 aacaaaatca agggaggtct ggaatgaaga aacaaagcaa gtacaaagag gtcgtttcct
    14761 atctgaaaaa tggtatcgag tctggacgat ttccgacggg tagtcgcctg ccttctatcc
    14821 gtcaactgag ccttgacttt cactgcagca aggacaccat tcaacgagcc ctgctggaat
    14881 tacggcacga acaatacctc tatgccaagc ctcagagtgg ctactatgta ttagaacaag
    14941 ggcaacatca agacctagaa atcgaggtta ccgacgaaca tgccagtgcc tatgacgatt
    15001 tccgactctg tgtcaatgaa accttgattg gccgagaaaa ctacctcttc aactactatg
    15061 acaatcaaga aggattagaa gacctaagac agtccattca caaactcctc tttgagcaag
    15121 ctctctactg caaggctaac caactagtac tgacttctgg aacccaacaa gccttgttta
    15181 tcctctctca aatatccttt cctagacaag ccaaggaaat cttggtggaa cagccaacct
    15241 accatcggat gaatcgcctc ttgattgcac aggggctgga ctatcaaacg attgaacgag
    15301 gcattgatgg gattgacttg gaggagctgg aaggccactt caaaacagga aaaattaagt
    15361 ttttctacac cattccccga tttcactatc ccctgggaca ttcctattct gagcaagaca
    15421 aacgatctat tcttaactta gctgccaagt atgatgtcta tatcgtagag gacgattatc
    15481 tgggtgattt ggactccaag aagggccaaa ccttccacta tcttgataca gaggagcgtg
    15541 tcatttatat caagtccttc tcgaccagcc tttttcctgc ccttcgtatt acagcactca
    15601 ttcttccaaa tgctatcaaa gaagcatttg tggcctacaa aaatatccta gactacgaca
    15661 gcaacctcat tatgcaaaag gccctgtcac tctatattga cagtcaattg tttgaaaaaa
    15721 atcgtttggc tcgcttgacc aatcatgaat cttaccaaaa acaaatcgag gaaaggataa
    15781 ctaaaacacc ttgtcccctt cctcattatt ccctacacga tggtttattg ctagacctga
    15841 gacagtatcc taaaatcgcc agtctcaaac acagtcaact gggcttggac ttctttgaag
    15901 aggcctattt aagcacctgt ccttatcaat ttgccaaggt gtccctagac aatctggaaa
    15961 atgttttaaa ctatttaaaa gcagaattgg aatgacatcc aactctgctt tttcttattc
    16021 ttcaacttca gttagacttg ctgctttttc aagatagctt tggtaagttc cttcatcctt
    16081 aagtttttgg acgaccttgt ctaccacttc tttcaaatca tcactatttt ttctaagagc
    16141 aacggcattg gcgtcgccgt ccttcatctt caagctgaca gttgcgacag ctaagccagc
    16201 gtttttagca gcataactaa gtgcaacagg ctcatccata tgaacagcat ctatttttcc
    16261 agcctgcaat tcattgactg cttcacccat attagttagg gaagttaatt gaacttttgg
    16321 caattgttcc ttgaccattg attctggaac agtccctttt tgggctgcaa tattagcact
    16381 ttctaggcta gttaaatcct tgtatttttc cacatcagcc ttacgaacca agaaactaat
    16441 cttgttttca tagtatggga ttgaaaaatc aaagacttct tttctctcgt cagtagcact
    16501 aattcctgca actgctaggt cagccttacc agtttgaaga ctggtcaaaa cattgtcaaa
    16561 actcatgctt gagatttcca acttaacccc aagttcatca gcgatagcct gagccatgtc
    16621 gatgtctgca ccgactacct ggttctttcc atcaaccaat gattgaaatt caaagggtgc
    16681 atagtcagga ctggttgcca caactaattt ccctttttgt ttgatagcat ccacagcaga
    16741 ctgagaacca ttagaacctg actgacaagc tactaagaag aaacttgcaa gaaaattaca
    16801 taaaacaaat atccattttt ttgatttcat aaataaacaa cctctctgtt aattttgtat
    16861 aattataacg atatccaagt tacttgtcaa gtgtttttta aatttttatc tcaaaaatat
    16921 tttttcgttc aaaaaaagga gccatcagtt gatttcaagc tcccttttat acagaattaa
    16981 actattttat agttcgacaa tcttacctgt ttcaaagtag acaacccatt cacagatatt
    17041 tttagcatag tcaccgatac gctccaagta ggaaataact tggaaataat cacgacccgt
    17101 aacaatggct tctggatttt tcttaatctc ttcagtcgca aggtcacgga tagtttcaaa
    17161 atagtggtta atttgctcat ccatggaggc cacccggtat gcgtcgtcaa cagaaccatt
    17221 aagataaaga tcaagtgctg cttccacaac gcttttaact tcacgtccca tttttttaat
    17281 ttcttcctct acagctggaa tgcgctcttc ccccttcata cggatggttg cctgggcaat
    17341 ggctacagcg tgatccccca tacgctccac atctgataca gccttaagga cagtcaagac
    17401 tgtacgcaaa tcttgagaga ctggttgttg gagtgcgatc atttcaaatg atttcttttc
    17461 cagtttcact tcgtattcat ttacttctgc atcatcttcg atgacctctt ttgccaggtc
    17521 acggtcatgc gtgacaaaag cacgtaccgt acgattgatt tgtgagagca cttcttgtcc
    17581 catagcgtag aactggttat gtaatttctc taaatcttct tcaaattgag atcgtaacat
    17641 ctttcatctc cttatccaaa ttttcctgta atatagtctt ccgtttcctt gtgttgggga
    17701 tcaaggaaca tctgcttggt atcattaaat tcaatcaaat ctccatctag gaaaaatcct
    17761 gtcttatcag agatacgtga agcttgctgc atggaacggg ttaccagaag catggtgtac
    17821 ttgtctttta gaccatacaa ggtttcctca attttaccag ctgaaatcgg atccaaagcc
    17881 gaagttggct catccaagag gatgatttta ggactagttg ccaagacacg ggccacgcag
    17941 acacgctgct gttgaccacc tgacaatcca atagctgaat catatagacg atccttgacc
    18001 tcatcccaga tagaggcacc ttgcaaggct ttttctacgg cttcatccag aacctgctta
    18061 tccttaattc cattgatacg aagcccgtag acaacattct catagatagt catagggaaa
    18121 ggattaggtt gttggaaaac cattccgatt tccttacgta attcaaccgt atctgtacgc
    18181 ggactgtaga tgttgtgacc attgtacacc acggatccag ttgtggtcac ctctggattg
    18241 agatctccca tgcggttgag agacttgagg agggttgact tccctgatcc agatggacca
    18301 atcaaggctg taatttcctt aggttggaaa gatagggaaa cactattcaa agccttcttt
    18361 ttattataat aaacggacag gtctgatacc tgtaaaatcg catctgtcat acggtttcct
    18421 ttctaaccaa agtgaccaga tacatagtca ttggtggact gtagcttggc attttggaaa
    18481 atagttgcag tcttgtcata ctcaatcaaa tcacccaagt aaaagaagcc tgtatagtca
    18541 cttgcacgag cagcctgctg catattatgc gttacaatga tgatggtaaa gtttttcttg
    18601 agctcaaaca tggtctcttc tagttgcatg gtcgcaatcg gatccaaggc tgaggctggc
    18661 tcatccatta agaggatatc tggcttaaca gagatggcac gagcgataca gagacgttgt
    18721 tgctgaccac ctgataaggt caaggctgac ttgtggagat cgtctttaac ctgatcccag
    18781 agggcagcct gacgaaggga ggtttctacg atttcatcta ggacttgctt atccttaact
    18841 ccagcacgtt catgcgcaaa ggtaatatta cggtaaattg acttagcaaa tggattggga
    18901 cgttgaaaaa ccattccaat gtgtttacgc atttcataaa cgttgatttc tggacggttg
    18961 acatcaattc cacgatagag aatctgccca gttactttag caatatcaat agtatcattc
    19021 atgcgattga gactgcgtaa gtaggtagat ttccccgatc ccgacgggcc aatcaaagct
    19081 gtaattttat ttctttcaaa ttgcatatca atccccttaa tggattcatt tttaccatag
    19141 taaacatgga catccttagt agaaagggct actttttctt caggaaaggt aaggatatgc
    19201 ttctcatccc agttatatgt tgacatggct tctcctttag gcagcggtta atttcttgtg
    19261 tagatagctt ccgaacttac gagctccaaa gttaaaaatc aggataaaga tcaggagcac
    19321 agcggcagaa cctgctgata caatggttcc atctggaata gtgccttcac tattgacttt
    19381 ccagatatgg acagccaagg tttctgcttg acggaagata gagatggggc tagtcacact
    19441 gaggatattc cagttagacc agtcaagagc tggcgccgat tgccctgctg tatagatcag
    19501 agctgcagct tcgccaaaga tacgaccaga tgccaagacg acacccgtta caatacctgg
    19561 aagcgcttcc ggaataacaa catgaaccac tgtctcccag cgagaaatcc caagagccag
    19621 accagcctca cgttgggtat ggtgaacgtg tttcaaacta tcctctacat tacgcgtcat
    19681 ctgaggcaag ttaaagactg tcaaggccaa ggcacctgaa atgattgaaa atccatactc
    19741 aaactggact acaaagatca agtaaccaaa gagacccacc accactgatg gtaaagagga
    19801 caaaatttca atacaagtcc gcacaaagtt ggtaacagga ccttttttag catattcagc
    19861 caagtaaatc ccagctccca tagaaagagg tacagaaata atcaaggtaa tgaccaatag
    19921 gaaaaaggaa ttgtaaagct gaatgccaat cccaccacct gcttgaaaag cagaagacct
    19981 tccagtcaag aaagaccaag agatatgggg caagccccga accaagatat agagaatcaa
    20041 ggaagccaag attgtcacaa tgatgctagc aatcgtatag aggacagctg ttgcaagttt
    20101 atctaatttc ttagcgcgca taatttttct ttcctctttc tttcgtaatc aatttaatca
    20161 cactgttaaa aactaagctc atcaagagca gtaccaaggc cagtgaccag agaacattat
    20221 tatttacagt tcccatgaca gtgttcccaa ttcccatagt taatatagaa gttaaagttg
    20281 cagctggtgt ggtcaaggaa gttgggataa cagctgagtt tccgacaacc atctggatag
    20341 ctagagcctc accaaaggca cgcgccatcc caaagaccac tgcagtgaaa ataccagaac
    20401 gggccgcctt caagatcaca cgccagatag tctgccagcg agtggctccc atagcgaaac
    20461 tggcttcacg ataataacga ggaaccgcac gcaagctatc cgttgtcata aaggttacgg
    20521 tcggcaaaat catgacaaag aggacggaaa tccctgacaa aatcccaaaa ccagtcccac
    20581 caaagacact gcgaacaaag ggaacgacga cttgcaagcc aataaatccg tacactactg
    20641 aaggaatccc aaccaggagt tcaatagctg gttgcaaaat cttcgcccct tttggtgata
    20701 cttcggtcat aaaaactgct gcaccaatag caaagggtgt tgcgataagg gctgagagaa
    20761 tggtaacgat aaaggaaccc aaaatcatag gaagggcacc aaattcttta ctagaaggat
    20821 tccaagttcc tcccaaaaga aagtcaaaga tattcacacc attgacaaag aaggtcgaca
    20881 agcctttttg cgctacgaaa accaaaatca tggccacaag gatgactatc aaagaaagac
    20941 aggcaaaggt caaacctttt cctaatttct ccagacgaga attctttgat ggaagcaaca
    21001 ttttcttagc taattcttct tgattcatta ttgtctccct tccaacactg tcacagttcc
    21061 ggcagcatct ttttcaacct tcatttcctt aatcggaata tacttcaatc ctttgacaat
    21121 cccttcttgg gtctcatccg agagaacaaa attgagaaat tctgcagcca actcattggg
    21181 ctgccccaat gtatacatat gctcataaga ccacaagggc caattattgc tacttatatt
    21241 ttctggactt aagtcatagc cattcaactt catgcttttg accgaatcat ctatataggt
    21301 aagagataaa taagagatag ctcctggact ttttgatacg attgatttta ccgctccatt
    21361 tgaatcctgc tcctgacttt gcatggcaga ctgaccttcc ataatgacag tatcaaaggt
    21421 agcacgagag ccagagccgg ctgcccgatt gataacagag atgggtaagt ccttaccacc
    21481 aacctctttc caattggtta cctcacctat gaagatttga cgaagttgct ctgtcgttag
    21541 gttatcaaca tcaacctcct tattgacaat cagagccaag ccagctaccg cgaccttgtg
    21601 gtcaacaaga gcagaagcat caattccgtc tttttcctca gcaaatacat ctgagtttcc
    21661 tatatcaact gccccagact gaacctggga caagcctgta ccagaacctc ccccttggac
    21721 attgaccgtt tttccaacat ggatcgtgcc aaattcatct gccgctactt caaccaaggg
    21781 ttgcaaggca gttgagccaa cagccgttat ggattctcca cgatcaatcc agctagcaca
    21841 gcctactaaa caagccgtca gccaaaaagc gataagagac agagcaagct tttttctttt
    21901 tttcactgtt tttctcctcg aaaataatta tgaatactgt gaatttttta agtagttctt
    21961 tatgagttga cgcatgaatt cttaccaaat ttctgcgcaa ttgattattt atataatata
    22021 ggctatatta ctctttccta acctcctttt ttcatatgtg gataaaatct cttgtctatc
    22081 ccttccccca ttgtcaccca ttatagtcat ttcgtgtctc tttttcccct ttttaatgca
    22141 agggaaatta ctctccttag atgataatcc aaaagctaga aaggtatctc aaacctctct
    22201 actctcccag actagtttac aactaaaagg aaaagattct attttatgag aaatctagtt
    22261 tacaagcggt aagaacgcta ataactaaac ttcttgtact ctttgaaaat ctcttcaaac
    22321 cagtgttttg agctatctat ggctagcttc ctagtttgct ctttgatttt cattgagtag
    22381 taaaactaca tgtaatggca atcaagatat caagaatcat cctactaaaa aaatccatac
    22441 tttcactata acatagaata agatatttga ctagcatttt catttgaatc tgaggccttt
    22501 tggaaaataa tttttcaaaa catttccagt aacctttgca aagcccaagc cattgccttt
    22561 aaccaaaact tggtaccaac catttggcag actttctgcc agctgaacgg tttctccagc
    22621 cgcatacttg acaaacgctt cttggccaat ttcaaccgac tgttcgacct gactcggttt
    22681 caaggctaaa ccaagagcga aactgggctc aaagcgtttc ttcttaaaag tacccagatg
    22741 cagtccattg cgagcaatct tgagcttccc taaatctggc aaaagttctg gcaagagata
    22801 aagctggtct ccaaaaatct gcaagatacc cggtagattg accttcaaat ggttttgggc
    22861 aaattcctgc cacaaggcaa cttgttcacg gctgaggtta ctcttacttg ccttaaattt
    22921 aggagctgga ttgttaccct taaactgtag atgggcaaca aactgaccct ctcccttaaa
    22981 ctgatgaggg tacatccgag ccgtttctgg caggtcaata ccagctacca ttccattgat
    23041 atgctctact ggcaacaagt caaaatcata ctcttccagc aaccaattga caatctcttc
    23101 gttttcctcg ggtgcccagg tacaggtcga ataaaccaga cgaccacctt cagctaacat
    23161 ggtcactgca tcctccagaa tttctctttg caagctagca cattgactcg gataatctaa
    23221 gctccaatag tccatagcat caggttgctt acgaaacatt ccttcaccag agcaaggggc
    23281 atcaagaacg attaagtcaa aatagccttt aaagaccttg accaagcggt cggcagattc
    23341 attggtcacc acgacatttg tcgctccaaa acgctccatg ttttcaacca aaatcttagc
    23401 ccgtttgctt gaaatttcat tggaaacaag tagcccctcc cctgctagat aggctgccag
    23461 ttgagttgat ttgccccccg gtgcagcagc caagtccaag accttcatac caggactggg
    23521 ttgggctact tgagccacca tttgagcagc aggttcttgc gaataaacta aacctgtagc
    23581 atgctcaggc gatttccctg aaaccttccc atagtggccc caaggggttt gagtaatggc
    23641 atcagaaaag gaaagttgct cttcttttaa gggattgacc cgaaaggccg aaaccgcttc
    23701 ctcctcaaaa gaggcaagaa aatctcttgc ctcatctcct agtatttctt tatatttttc
    23761 aacaaatcct tctgaaaatt tcatttaagt tcttttcctt tcgtaaatat aggactgaat
    23821 ttcctcctgc atctcaagag gcaccatcat gaccggctgt ctggtttgaa aatcaggagc
    23881 ttcaccaaaa agggtcacaa cccgatagcc cagactttcc cctaaaatac tagctgcggc
    23941 ataatcccat ggttgcagat aagtgagata ggtcaacaaa cgccctgaca aaatcttggc
    24001 aaaactaatg gccgcacttc catagacacg aacaccaaga accgctcggc tcaaatcagc
    24061 cagcccccat tcattggttt ccagcatacc actattccct gcaatgagaa aatctccaag
    24121 tggtttagtt ttaaaaggag ctaggggcct atcatttaga caaactggaa attccccacc
    24181 accgtggtaa caatcccctt tgaccacatc ataaatcaga ccaaactgtc cctgaccatt
    24241 ttcaaaataa gccatcataa cagcaaaatc ttcctgctgg gctacaaaat tattggtacc
    24301 atcaatgggg tcaatgaccc aaaccttgcc ctcttgaacc gaggctcgca gacaaccttc
    24361 ttcagcacaa atcttatcct caggataacg ggacaaaatc tcaccaacca agagttcctg
    24421 aacttctttg tccagtctgg tcaccaaatc tgttggagag gacttggttt caacacgcaa
    24481 gtcttcctgc atatggtcaa gaatgtactg gcctgctttc ttaacaagct ctttagcaaa
    24541 ttcaaattta ctttccaaga gaaatctttc cttccccttt ttctttggca gcctgaactg
    24601 ctcggtaaag tgaatagcca ctaaccgttt caaattctcg tcccaaacgt ttctcttcgc
    24661 tcttgcttgg cacgaccgct ttgaatccct tataggagtc cagtaacttt ttagcctcta
    24721 ctttgccttc ataggcagct tcaacatcat taaaaaaaga aagcactgaa gcaagttctt
    24781 cagtgctcca cgacaaatct agtgggtaac tatactgttt gttcattaac taataccagc
    24841 tctcattctt gcttctttta gttcttgctt acgataacta cgagggagaa aagcacgaat
    24901 ctcatcttca ttaaaaccga tttgcatacg cttggcatca ataataattg gacgacgcaa
    24961 aagactagga tactgctcaa tcaaatgaag caattccgat accgaaatac tctctacatc
    25021 aatattcaat ttttgaaaaa tttttgaacg agttgaaatg atgtcatcag taccattttc
    25081 ggtcaaggaa aggatgtgtt gcaattcttt tcttgttaaa ggactggtca taatattgtg
    25141 ttccacaaag ggaaccttat gtttttctaa ccaggcctta gccttacgac atgatgtaca
    25201 gctcggtgat agaaatagtg taatcatgct tttctcttct tatctatact ttgctacttc
    25261 tattatacaa aaaaataaag cgcttgacta gggattttta gaaaaaaagc ctattttttc
    25321 aagaaaaata ggctgtttgc gaacgattga cacaattgga tttggttaat tcactcttaa
    25381 cgatggtttt a
//
LOCUS       DAVHEX010000030        25390 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVHEX010000030 DAVHEX010000000
VERSION     DAVHEX010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25390
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.030"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            15..491
                     /locus_tag="VVL45_001189"
     CDS             15..491
                     /locus_tag="VVL45_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU4187370.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEQLFDQVATLLEEREIVTPTYREA
                     LITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDK
                     EVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEKLYAYIAETTA"
     gene            529..834
                     /locus_tag="VVL45_001190"
     CDS             529..834
                     /locus_tag="VVL45_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU4187371.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSAPVFDNIEAAIKEHGLS"
     gene            882..2357
                     /locus_tag="VVL45_001191"
     CDS             882..2357
                     /locus_tag="VVL45_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEU4187372.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNVVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLLIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLQGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVAFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
     gene            2662..9363
                     /locus_tag="VVL45_001192"
     CDS             2662..9363
                     /locus_tag="VVL45_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU4187373.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQASSQEKPLKEDAKAVTNEEVNQMIENRKVDFNQNWYF
                     KLNANSKEAIKPDADVSTWKKLDLPYDWSIFNDFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLYKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SNQVTVFTGKKEGQEKTVLGTEVPKVQTIIGEAPEMPTTVPFVYSDGSRAERPVTWSS
                     VDVSKPGIVTVKGMADGREVEARVEVIALKSELPVVKRIAPNTDLNSVDKSVSYVLTD
                     GSVQEYEVDKWEIAEEDKAKLAIPGSRIQATGYLEGQPIHATLVVEEGNPAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAKNAAVTVLQASAANGMRASIFIQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLNAEYEGAKGQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQEPSLPATVTVEYDKGFPKTHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRPEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRTNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAVRIRMVKADNKRGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVPAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKSLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKDLTVEWEEVPAEN
                     LTKAGQFTVRGRVLGSNLVAEVTVRVTDKLGETLSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDYSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQGKVQFFA
                     DSGTDAPSKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKAIKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSFPVLVRLVSESGKQVKEYR
                     IHLTKEKPVSEKTVAAVQEDLPKIEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKVGK
                     ERIFTAINPDGSKEEKLREVVEVPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPNTGSAASQAAVAAGLALLGLSAGLVVTKGKKED"
     gene            complement(9611..10467)
                     /locus_tag="VVL45_001193"
                     /pseudo
     CDS             complement(9611..10467)
                     /locus_tag="VVL45_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(10679..11038)
                     /locus_tag="VVL45_001194"
     CDS             complement(10679..11038)
                     /locus_tag="VVL45_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEU4187374.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSFLAIILVFFFLLKKRSFYYPKFIKFFWRAGFL
                     LTLIMYIEMIVELFLMK"
     gene            11130..12086
                     /locus_tag="VVL45_001195"
                     /pseudo
     CDS             11130..12086
                     /locus_tag="VVL45_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            12070..12627
                     /locus_tag="VVL45_001196"
     CDS             12070..12627
                     /locus_tag="VVL45_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4187375.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQAGLTNAQLILQGHETLDQFVTKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            12620..12874
                     /locus_tag="VVL45_001197"
     CDS             12620..12874
                     /locus_tag="VVL45_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187376.1"
                     /translation="MDKQYLHEKLDVMRQNFVESTHHERAVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKDQKEIFESCCKKD"
     gene            12886..14940
                     /locus_tag="VVL45_001198"
     CDS             12886..14940
                     /locus_tag="VVL45_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU4187377.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKDKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNIN
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            15119..15979
                     /locus_tag="VVL45_001199"
     CDS             15119..15979
                     /locus_tag="VVL45_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU4187378.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLGFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNLSNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            16190..16900
                     /gene="ccdA2"
                     /locus_tag="VVL45_001200"
     CDS             16190..16900
                     /gene="ccdA2"
                     /locus_tag="VVL45_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEU4187379.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGFLVLFGNASILSQLFE"
     gene            16920..17486
                     /locus_tag="VVL45_001201"
     CDS             16920..17486
                     /locus_tag="VVL45_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEU4187380.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            17497..18609
                     /gene="msrB"
                     /locus_tag="VVL45_001202"
     CDS             17497..18609
                     /gene="msrB"
                     /locus_tag="VVL45_001202"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU4187381.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQSISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
     gene            18967..19704
                     /locus_tag="VVL45_001203"
     CDS             18967..19704
                     /locus_tag="VVL45_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4187382.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            19701..21392
                     /locus_tag="VVL45_001204"
     CDS             19701..21392
                     /locus_tag="VVL45_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU4187383.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIDTGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYHMKIDSRPQKGTKVEIYINRIETS"
     gene            21508..22071
                     /locus_tag="VVL45_001205"
     CDS             21508..22071
                     /locus_tag="VVL45_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001006373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEU4187384.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGALFVLGLSSSEVLGGHIG
                     KESSQEIGELIVETILDILGSRGIHLAVQGCEHVNRALVVERQVAEQFGLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKF"
     gene            22093..>25390
                     /locus_tag="VVL45_001206"
     CDS             22093..>25390
                     /locus_tag="VVL45_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HEU4187385.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGLLTVGSLLLIYKRKK
                     IASVFLVGAMGLVVLPSAGAVDPVATLALASREGVVEMEGYRYVGYLSGDILKTLGLD
                     TVLEETSAKPGEVTVVEVETPQSITNQEQARTENQVVETEEAPKEEAPKTEESPKEEP
                     KSEVKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKPEEKVAVKPESQPSDKPAEES
                     KVEQAGEPVAPREDEQAPVEPEKQPEAPEEEKAVEETPKQEESTPDTKAEETVEPKEE
                     TVNQSIEQPKVETPAVEKQTEPTEEPKVEQAGEPVAPREDEQAPTAPVEPEKQPEVPE
                     EEKAVEETPKPEDKIKGIGTKEPVDKSELNNQIDKASSVSPTDYSTASYNALGPVLET
                     AKGVYASEPVKQPEVNSETNKLKTAIDALNVDKTELNNTIADAKTKVKEHYSDRSWQN
                     LQTEVTKAEKVAANTDAKQSEVNEAVEKLTATIEKLVELSEKPILTLTSTDKKILERE
                     AVAKYTLENQNKTKIKSITAELKKGEEVINTVVLTDDKVTTETISAAFKNLEYYKEYT
                     LSTTMIYDRGNGEETETLENQNIQLDLKKVELKNIKRTDLIKYENGKETNESLITTIP
                     DDKSNYYLKITSNNQKTTLLAVKNIEETTVNGTPVYKVTAIADNLVSRTADNKFEEEY
                     VHYIEKPKVHEDNVYYNFKELVEAIQNDPSKEYRLGQSMSARNVVPNGKSYITKEFTG
                     KLLSSEGKQFAITELEHPLFNVITNATINNVNFENVEIERSGQDNIASLANTMKGSSV
                     ITNVKITGTLSGRNNVAGFVNNMNDGTRIENVAFFGKLHSTSGNGSHTGGIAGTNYRG
                     IVRKAYVDATITGNKTRASLLVPKVDYGLTLDHLIGTKALLTESVVKGKIDVSNPVEV
                     GAIASKTWPVGTVSNSVSYAKIIRGEELFGSNDVDDSDYASAHIKDLYAVEGYSSGNR
                     SFRKSKTFTKLTKEQADAKVTTFNITADKLESDLSPLAKLNEEKAYSSIQDYNAEYNQ
                     AYKNLEKLIPF"
BASE COUNT         7890 a         4739 c         5549 g         7212 t
ORIGIN      
        1 gaagggaaaa acttatggta ttagattatt tctttgacaa aaaccttgtg ttttgcttag
       61 aagcggataa tcaagaacaa ctctttgatc aggttgcaac tttattggaa gaacgagaaa
      121 ttgtcactcc aacttatcgt gaagctttga ttacgcgtga aaagtcattt ccaactggtt
      181 tagatatgga atttctagga aaggacttgc caaatgtagc gattcctcat acagatattg
      241 ttcataatct agctgagaaa gtggtggttg ttcgattaga aaaaccagta acttttcaca
      301 atatgatagc tcctgataag gaagtagaag tatccttgct cttctttatc atcaataact
      361 caagttcaag tcaaacaaat attctggctc agttgatgga ctttttcaca ggaaatggac
      421 atcttgaaga cctatcaaaa atttccgaac cagaaaaact ttatgcttac attgctgaaa
      481 caaccgctta atcttgtcta ttaaaaaata aaatcggagg aaatctaaat gattaaaatt
      541 cttgctgcct gcggtgcagg tgttaactca agtcaccaaa ttaaaagtgc tctagaagaa
      601 gaactttcaa accgtggtta tgatgttcac tgtgatgcag tcatggtgaa agatgtaaac
      661 gaagacctta tgaaaggtta cgatatcttt acaccaatcg ctgcaacaga ccttggtttt
      721 gaaccaggta tcccagttat cgaagctgga ccaatcttat tccgtatccc agcaatgagc
      781 gctccagtat ttgacaatat tgaagcagct attaaagaac acggattaag ctaattctaa
      841 tttgttaaca ttcatataac ataaaaaagg gaggaactgt tatggatgca atctttgacc
      901 taatcggaaa ggttttcaat cccatcttag aaatgggtgg acctgtcatc atgttaatca
      961 ttttgacagt attggcttta ctttttggag tgaaattctc caaagcgctt gaaggtggta
     1021 tcaaacttgc catcgctctt acaggtatcg gtgctatcat cggtatgcta aatggtgctt
     1081 tctcagcatc acttgcaaaa ttcgttgaaa acactggtat ccaattgagt attaccgacg
     1141 ttggttgggc accacttgca acaatcactt ggggttctgc ttggacactt tacttcttgc
     1201 tcatcatgtt gattgtcaac gtagtgatgc ttgctatgaa gaaaaccgat acacttgatg
     1261 tcgatatctt tgatatctgg cacttgtcta tcacaggtct cttgattaaa tggtatgctg
     1321 ataacaatgg tgtgagtcaa ggggtttcac tcttgattgc tacagcagct atcgttcttg
     1381 tcggtgtgtt gaaaattatc aactctgact tgatgaaacc tacatttgat gaccttctta
     1441 acgccccaag ttcatcacca atgacatcaa ctcacatgaa ctacatgatg aacccagtta
     1501 tcatggtttt ggataagatt tttgaaaaat tcttcccagg ccttgataaa tatgactttg
     1561 atgctgctaa attgaacaag aaaatcggtt tctggggatc taaattcttc atcggtttca
     1621 tccttggtat cgttatcggt attatgggaa ctccacatcc aattgcaggt gttgcagatg
     1681 cagataaatg gcgtcttgtt atcaaaggat ggttgtctct tggtttgact gccggtgtat
     1741 ctttggaact cttctcactt atcggttcat ggttcatcgc agccgtagaa ccactatcac
     1801 aaggtattac aaacgttgct actaaacgtc ttcaaggacg taaattcaat atcggtcttg
     1861 actggccatt catcgctggt cgtgctgaaa tctgggcttg tgccaacgta cttgcaccaa
     1921 tcatgttgat tgaagcagtg cttctttcaa aagttggaaa tggtatcttg ccacttgcag
     1981 gtatcatcgc tatgggtgtt actccagctc tcttggttgt aactcgtggt aaattgctcc
     2041 gtatgattat cttcggaaca ctcttgttgc cactcttcct tctttcaggt acacttattg
     2101 caccatttgc aacagaactt gctaaaggtg taggtgcctt cccagaaggt gtgagccaaa
     2161 ctcaattgat tactcactct actcttgaag gaccaatcga aaaacttctt ggttggacaa
     2221 ttggtaacac tacaactggt gatatcaaag caatccttgg tgcagtagcc ttccttgtat
     2281 tctatatcgg tatctttgct tggtacagaa aacaaatgat caaacgtaac gaagagtacg
     2341 cagcaaaagc aaaataatgc gctcctataa aatataaact caatgaaaat caaagagcaa
     2401 actaggaagc tagccgcagg ttgctaaaaa cacagttttg aggttgtaga taagactgac
     2461 gaagtcagct caaaacatgg ttttgaggtt gtagatgaaa ctgacgaagt cagtaatcat
     2521 acatacggca aggcgacact gacgaagttt gaattggatt ttcgcagagt gtaaattgtt
     2581 gaaactaggt tgtcatacca tgtataccac ttgggctttg gcagtagcta actgcactaa
     2641 atataatata aggagggttt tatggggaaa ggccattgga atcggaaaag agtttatagc
     2701 attcgtaagt ttgctgtggg agcttgctca gtaatgattg ggacttgtgc agttctatta
     2761 ggaggaaata tagctggaga atctgtagtt tatgcggatg aaacacttat tactcatact
     2821 gctgagaaac ctaaagagga aaaaatgata gtagaagaaa aggctgataa agctttggaa
     2881 actaaaaatg tagttgaaag gacagaacaa agtgaaccta gttcaactga ggctattgca
     2941 tctgagaaga aagaagatga agccgtaact ccaaaagagg aaaaagtgtc tgctaaaccg
     3001 gaagaaaaag ctccaaggat agaatcacaa gcttcaagtc aagaaaaacc gctcaaggaa
     3061 gatgctaaag ctgtaacaaa tgaagaagtg aatcaaatga ttgaaaacag gaaagtggat
     3121 tttaatcaaa attggtactt taaactcaat gcaaattcta aggaagccat taaacctgat
     3181 gcagacgtat ctacgtggaa aaaattagat ttaccgtatg actggagtat ctttaacgat
     3241 ttcgatcatg aatctcctgc acaaaatgaa ggtggacagc tcaacggtgg ggaagcttgg
     3301 tatcgcaaga ctttcaaact agatgaaaaa gacctcaaga aaaatgttcg ccttactttt
     3361 gatggcgtct acatggattc tcaagtttat gtcaatggtc agttagtggg gcattatcca
     3421 aatggttata accagttctc atacgatatc accaaatacc tttacaaaga tggtcgtgag
     3481 aatgtgattg ctgtccatgc agtcaacaaa cagccaagta gccgttggta ttcaggaagt
     3541 ggtatctatc gtgatgtgac tttacaagtg acagataagg tgcatgttga gaaaaatggg
     3601 acaactattt taacaccaaa acttgaagaa caacaacatg gcaaggttga aactcatgtg
     3661 accagcaaaa tcgtcaatac ggacgacaaa gaccatgaac ttgtagccga atatcaaatc
     3721 gttgaacgag gtggtcatgc tgtaacaggc ttagttcgta cagcgagtcg taccttaaaa
     3781 gcacatgaat caacaagcct agatgcgatt ttagaagttg aaagaccaaa actctggacc
     3841 gttttaaatg acaaacctgc cttgtacgaa ttgattacgc gtgtttaccg tgacggtcaa
     3901 ttggttgatg ctaagaagga tttgtttggt taccgttact atcactggac tccaaatgaa
     3961 ggtttctctt tgaatggtga acgtattaaa ttccatggag tatccttgca ccacgaccat
     4021 ggggcgcttg gagcagaaga aaactataaa gcagaatatc gccgtctcaa acaaatgaag
     4081 gagatgggag ttaactccat ccgtacaacc cacaaccctg ctagtgagca aaccttgcaa
     4141 atcgcagcag aactaggttt actcgttcag gaagaggcct ttgatacttg gtatggtggc
     4201 aagaaacctt atgactatgg acgtttcttt gaaaaagatg ccactcaccc agaagctcga
     4261 aaaggtgaaa aatggtctga ttttgaccta cgtaccatgg tcgaaagagg caaaaacaac
     4321 cctgctatct tcatgtggtc aattggtaat gaaataggtg aagctaatgg tgatgcccac
     4381 tctttagcaa ctgttaaacg tttggtcaag gttatcaagg atgttgataa gactcgctat
     4441 gttaccatgg gagcagataa attccgtttc ggtaatggta gcggagggca tgagaaaatt
     4501 gctgatgaac tcgatgctgt tggatttaac tattctgaag ataattacaa agcccttaga
     4561 gctaagcatc caaaatggtt gatttacggt tcagaaacat catcagcaac ccgtacacga
     4621 ggaagttact atcgccctga acgtgaattg aaacatagca atggacctga gcgtaattat
     4681 gaacagtcag attatggaaa tgatcgtgtg ggttggggga aaacagcaac cgcttcatgg
     4741 acttttgacc gtgacaacgc tggctatgct ggacagttta tctggacagg tacggactat
     4801 attggtgaac ctacaccatg gcacaaccaa aatcaaactc ccgttaagag ctcttacttt
     4861 ggtatcgtag atacagccgg cattccaaaa catgacttct atctctacca aagccaatgg
     4921 gtttctgtta agaagaaacc gatggtacac cttcttcctc actggaactg ggaaaacaaa
     4981 gaattagcat ccaaagtagc tgactcagaa ggtaagattc cagttcgtgc ttattcgaat
     5041 gcttctagtg tagaattgtt cttgaatgga aaatctcttg gtcttaagac tttcaataaa
     5101 aaacaaacca gcgatgggcg gacttaccaa gaaggtgcaa atgctaatga actttatctt
     5161 gaatggaaag ttgcctatca accaggtacc ttggaagcaa ttgctcgtga tgaatctggc
     5221 aaggaaattg ctcgagataa gattacgact gctggtaagc cagcggcagt tcgtcttatt
     5281 aaggaagatc atgcgattgc agcagatgga aaagacttga cttacatcta ctatgaaatt
     5341 gttgacagcc aggggaatgt ggttccaact gctaataatc tggttcgctt ccaattgcat
     5401 ggtcaaggtc aactggtcgg tgtagataac ggagaacaag ccagccgtga acgctataag
     5461 gcgcaagcag atggttcttg gattcgtaaa gcatttaatg gtaaaggtgt tgccattgtc
     5521 aaatcaactg aacaagcagg gaaattcacc cttactgccc actctgatct cttgaaatcg
     5581 aaccaagtca ctgtctttac tggtaagaaa gaaggacaag agaagactgt tttggggaca
     5641 gaagtgccaa aagtacagac cattattgga gaggcacctg aaatgcctac cactgttccg
     5701 tttgtataca gtgatggtag ccgtgcagaa cgtcctgtaa cctggtcttc agtagatgtg
     5761 agcaagcctg gtattgtaac ggtgaaaggt atggctgacg gacgagaagt agaagctcgt
     5821 gtagaagtga ttgctcttaa atcagagcta ccagttgtga aacgtattgc tccaaatact
     5881 gacttgaatt ctgtagacaa atctgtttcc tatgttttga ctgatggaag tgtacaagag
     5941 tatgaagtgg acaagtggga gattgccgaa gaagataaag ctaagttagc aattccaggt
     6001 tctcgtattc aagcgaccgg ttatttagaa ggtcaaccaa ttcatgcaac ccttgtggta
     6061 gaagaaggca atcctgcagc acctgtagtg ccaactgtta ctgttggagg tgaagcagta
     6121 acaggtctta ctagtcgaca accaatgcaa tatcgtactc tatcttatgg tgcccaattg
     6181 ccagaagtca cagcaagtgc taaaaatgca gctgttacag ttcttcaagc aagcgcagca
     6241 aacggcatgc gtgcgagcat ctttattcag cctaaagatg gtggccctct tcaaacctat
     6301 gcaattcaat tccttgaaga agcgccaaaa attgctcact tgagcttgca agtggaaaaa
     6361 gctgacagtc tcaaagaaga ccaaactgtc aaattgtcgg ttcgagctca ctatcaagat
     6421 ggaacgcaag ctgtattacc agctgataaa gtaaccttct ctacaagtgg tgaaggggaa
     6481 gtcgcaattc gtaaaggaat gcttgagttg cataagccag gagcagtcac tctgaacgct
     6541 gaatatgagg gagctaaagg ccaagttgaa ctcactatcc aagccaatac tgagaagaag
     6601 attgcgcaat ccatccgtcc tgtaaatgta gtgacagatt tgcatcagga accaagtctt
     6661 ccagcaacag taacagttga gtatgacaaa ggtttcccta aaactcataa agtcacttgg
     6721 caagctattc cgaaagaaaa actagactcc tatcaaacat ttgaagtact aggtaaagtt
     6781 gaaggaattg accttgaagc gcgtgcaaaa gtctctgtag aaggtatcgt ttcagttgaa
     6841 gaagtcagtg tgacaactcc aatcgcagaa gcaccacaat taccagaaag tgttcggaca
     6901 tatgattcaa atggtcacgt ttcatcagct aaggttgcat gggatgcgat tcgtccagag
     6961 caatacgcta aggaaggtgt ctttacagtt aatggtcgct tagaaggtac tcaattaaca
     7021 actaaacttc atgttcgcgt atctgctcaa actgagcaag gtgcaaacat ttctgaccaa
     7081 tggaccggtt cagaattgcc acttgccttt gcttcagact caaatccaag cgacccagtt
     7141 tcaaatgtta atgacaagct catttcctac aataaccaac cagccaatcg ttggacaaac
     7201 tggaatcgta ctaatccaga agcttcagtc ggtgtcctat tcggagattc aggtattttg
     7261 agcaaacgct ccgttgataa tctaagtgtc ggattccacg aagaccatgg agttggtgca
     7321 ccgaagtctt atgtgattga gtattatgtt ggtaagactg tcccaacagc tcctaaaaac
     7381 cctagttttg ttggtaatga ggaccatgtc tttaatgatt ctgccaactg gaaaccagtt
     7441 actaatctaa aagcccctgc tcaactcaag gctggagaaa tgaaccactt tagctttgat
     7501 aaagttgaaa cctatgctgt tcgtattcgc atggttaaag cagataacaa gcgtggaacg
     7561 tctatcacag aggtacaaat ctttgcgaaa caagttgcgg cagccaagca aggacaaaca
     7621 agaatccaag ttgacggcaa agacttagca aacttcaacc ctgatttgac agactactac
     7681 cttgagtctg tagatggaaa agttccggca gtcacagcaa gtgttagcaa caatggtctc
     7741 gctaccgtcg ttccaagcgt tcgtgaaggt gagccagttc gtgtcatcgc gaaagctgaa
     7801 aatggcgaca tcttaggaga ataccgtctg cacttcacta aggataagag cttactttct
     7861 cataaaccag ttgctgcggt taaacaagct cgcttgctac aagtaggtca agcacttgaa
     7921 ttgccgacta aggttccagt ttacttcaca ggtaaagacg gctacgaaac aaaagacctg
     7981 acagttgaat gggaagaagt tccagcggaa aatctgacaa aagcaggtca atttactgtt
     8041 cgaggccgtg tccttggtag taaccttgtt gctgaggtca ctgtacgagt gacagacaaa
     8101 cttggtgaga ctctttcaga taaccctaac tatgatgaaa acagtaacca ggcctttgct
     8161 tcagcaacca atgatattga caaaaactct catgaccgcg ttgactatct caatgacgga
     8221 gattattcag aaaatcgtcg ttggacaaac tggtcaccaa caccatcttc taatccagaa
     8281 gtatcagcgg gtgtgatttt ccgtgaaaat ggtaagattg tagaacggac tgttgcacaa
     8341 ggaaaagttc agttctttgc agatagtggt acggatgcac catctaaact cgttttagaa
     8401 cgctatgtcg gtccagagtt tgaagtgcca acctactatt caaactacca agcctacgac
     8461 gcagaccatc cattcaacaa tccagaaaat tgggaagctg ttccttatcg tgcggataaa
     8521 gacattgcag ctggtgatga aatcaacgta acatttaaag ctatcaaagc caaagctatg
     8581 agatggcgta tggagcgtaa agcagataag agcggtgttg cgatgattga gatgaccttc
     8641 cttgcaccaa gtgaattgcc tcaagaaagc actcaatcaa agattcttgt agatggaaaa
     8701 gaacttgctg atttcgctga aaatcgtcaa gactatcaaa ttacctataa aggtcaacgg
     8761 ccaaaagtct cagttgaaga aaacaatcaa gtagcttcaa ctgtggtaga tagtggagaa
     8821 gatagctttc cagtacttgt tcgcctcgtt tcagaaagtg gaaaacaagt caaggaatac
     8881 cgtatccact tgactaagga aaaaccagtt tctgagaaga cagttgctgc tgtacaagaa
     8941 gatcttccaa aaatcgaatt tgttgaaaaa gatttggcat acaagacagt tgagaaaaaa
     9001 gattcaacac tgtatctagg tgaaactcgt gtagaacaag aaggaaaagt tggaaaagaa
     9061 cgtatcttta cagcgattaa tcctgatgga agtaaggaag aaaaactccg tgaagtggta
     9121 gaagttccga cagaccgcat cgtcttggtt ggaaccaaac cagtagctca agaagctaaa
     9181 aaaccacaag tgtcagaaaa agcagataca aaaccaattg attcaagtga agctagtcaa
     9241 actaataaag cccagttacc aaatacaggt agtgcggcaa gccaagcagc agtagcagca
     9301 ggtttagctc ttctaggttt gagtgcagga ttagtagtta ctaaaggtaa aaaagaagac
     9361 tagaaatttt gataccttct ttatcattaa agaccaaaga aaaactcttg tcctaaatag
     9421 aagataaaga gtctggaaca aaatagttct caaccacaaa aaagctagat atttccaatt
     9481 gcggaactct agctttttaa ttttgagtcg ttctcttatt gtatagtaga ttgaaactag
     9541 agtagtacac ttctacttct aaaacattgt tagaaatcga tttgactgtc cttatcgatt
     9601 tgtcctgttc ttatttcatt tcactatgtt ttaggaggtt attggccata agtaccaatc
     9661 ccatgtcaat tctcacttga cgcttgcccc tcagattaca tctcttatga cccaaacaag
     9721 cctttatctg cccaaagaca ggtttcacat caatcttgcg ttgagcgaaa atctgtctac
     9781 cttggggaga taaaagcgcc tgacattctt tagctttcaa gttttgatag cgttcgttca
     9841 tatacagtcc tttttgaggg gctgattcag gttcatcagc gtagtaaacc ttgatttcct
     9901 gttgaaagtc tgtctgtgtt ttctggtgct tgatatggtg aaaacgatag caccagccat
     9961 caggatgtat gtagctatcc tccttgtcat tatagtgcca attcgctcag tttttagctg
    10021 actctttata ccctctcttt tgtttcttat caaacatggc atatttaatc agaagggaag
    10081 tgatttcttc ttgaaaatag acttgtatct gttcttgaag tttggcggag aatttctctg
    10141 tcgttttctt ccacacaaaa ctatacttgt tggcattggc ttcaatctta gttccgtcaa
    10201 taaacaaaca atctaaggtc actaactctt ccatttttca atttttcgac cagagaaaat
    10261 cccttgtgaa taggcaaata gaagagtaga tacaagcatt ttagggtgat aagacgggcg
    10321 atcaaaggca tgataggagg tgtagaagtg acgttcctcc aaggtattca ccactttttc
    10381 aatagtaaag acgagatggt cttgtggtaa gaaggaactg atttctagtg gtaaagttgt
    10441 ttgatttgtg ttatagtgaa tatgcatggg atagcctttt taaatggttt attaagcaat
    10501 tctattttac caagactatc gtaaccatgc aaaaaaagca acggcaaatc cgttgctttt
    10561 tttggagata agagactatt gtcccaggct cttttctttg tataaaattg tatagtgtta
    10621 ttgtctatac tattttgtag agaaagcaaa aatccctaga aaatgcttag ggattcgact
    10681 atttcattaa gaacaattca acaatcattt ctatatacat gataagggtt aataagaatc
    10741 ctgcacgcca gaagaatttg atgaatttag gatagtaaaa gctgcgtttt tttaaaagga
    10801 agaaaaagac gagaataatg gctaggaacg agagggctag ccccagtcta gggaggaaat
    10861 tatgggtaaa ggttttagct gtaatcaagt aatactcaaa taccaagact ggaaaagcca
    10921 aatccgcaaa gtttcgtcct agttttttta atctaaaaag tttggttagg ataatgcaga
    10981 cgactaaggt cagtatcaat aataaaatag atgctaattt cattaaaatc atacccatat
    11041 tgtatcataa aaaaaggcta atggaaacga gaaacagagg aatttatagg aaagtcaggc
    11101 ttttgagatt gtgggtgatt tttgttataa tgaaagttat aaaatcttat aataccttga
    11161 atgattatta tcgaaaactg tttggagaaa agacttttaa agtccctatt gatgcgggat
    11221 ttgactgtcc caatcgtgat ggaactgtgg ctcatggagg ctgtactttt tgtacggttt
    11281 caggttctgg agatgctatt gtagccccag atgcgcctat ccgtgagcaa ttttataagg
    11341 aaattgactt tatgcaccgt aagtggccgg atgttcagaa gtatctggtt tattttcaaa
    11401 attttaccaa cacccatgaa aaggtggaag ttattcgaga gcgttatgaa caggctatca
    11461 atgagccagg tgtggtaggt atcaatattg gaacgcgccc agactgttta ccagacgaaa
    11521 ccatcgaata tttggctgag ttatcggagt gcatgcatgt gatggttaaa ttgggcttgt
    11581 agactactta tgaagcaact tctgacctga ttaaccgtgc gcattcttat gaattgtagg
    11641 tggaaacggt caagcgtttg agaaagtatc ccaagattga gattgtttcc catttgatca
    11701 atggtttgcc tggtgaaacc catgagatga tggttgaaaa tgtccgccgt tgtgtcacga
    11761 ataacgatat tcaagggatt aaactgcatt tgctccatct tatgacaaat actcgtatgc
    11821 aacgagatta ccatgaggga cgtttacaat tgatgagtca ggacgaatat gtcagggtca
    11881 tctgtgacca actggaaatc attcccaaac atatcgccat ccatcgaatt acaggagatg
    11941 cgcctaggga tatgctgctt gggcttatgt ggagcctcaa taaatgggaa gctctcaaca
    12001 gcattgagat ggagatgcga cgtcgtggaa gtgttcaagg atgcaaggct gtaaaacagg
    12061 agtttgaaaa tgaaaagacc acttgagatg gcacatgatt ttttggctga ggtcgtgaca
    12121 aaagaggatg tcgtagtgga tgcgactatg ggaaatggtc atgacacgct ttttttagcc
    12181 aagctagcca agcaagtcta tgcctttgat attcagaagc aagccttgga aaagacccaa
    12241 gagcgtttgc atcaggctgg cttgacaaat gcccagttaa tcttgcaagg ccatgagaca
    12301 ctggaccagt ttgtgacaaa agctaaggca gggattttta atttgggcta tttgccgtca
    12361 gctgataagt ctgtcatcac ccgaccgcag acaacgattg aggcattaga aaagctatgt
    12421 ggcttacttg tcaaaggtgg acgaattgct attatgattt actatggtca tgaaggaggc
    12481 gacctcgaga gagatgctgt cttggatttt gtgatccagt tgaaccaaca agagtacaca
    12541 gctgccattt accgaacttt aaaccaagtc aacaacccgc cgtttttagt gatgattgaa
    12601 aaattagaga gatacagaca tggataaaca atacctacac gaaaagctgg atgtcatgcg
    12661 ccagaatttt gttgaatcaa cccaccacga acgagcggtg ggcgtgctag accaagcgca
    12721 tatgagcaaa aaaatgctta aaatcaagaa aaaattagtt gctcttgaaa tggaacggtg
    12781 ccagagaaaa attgagcaca aagattgttc caagattgac caaaaaatca aagatcagaa
    12841 ggagatattt gaatcctgtt gtaaaaaaga ttaaggagga cgtgcgtgga attactgatt
    12901 tacctcattc tatttttact ggttttgatt gtctcaagta caaccaataa gctcctgccc
    12961 tttttgcctc tccctttggt gcagattctt ttgggaattg tgattggtct ctttttaccc
    13021 aatactgact ttcaccttaa tacggagttg tttttggcac tggttatcgg ccccttgctt
    13081 ttccgagagg ctgaagaagc agatgttacg gctattttaa aacactggcg aatcattgtt
    13141 tatctcatat ttccagtgat ttttatctcg accctgagtt tgggtggctt ggcccatctt
    13201 ctttggttca gccttccctt ggcagcttgc ttggctgttg gggcagccct tggtcctacg
    13261 gacttggtgg cctttgcctc tctttcggag cgttttagct ttcctaagcg cgtgtccaat
    13321 attcttaagg gcgaaggact cttgaatgat gcttctggtt tggtggcttt tcaggtagct
    13381 ttgacagctt ggacaactgg agctttttct ctggggcaag ctagcagttc gctcatcttt
    13441 tcaatcctag gcggtttttt aattggattt ttaacagcca tgaccaaccg cttcctccat
    13501 accttcttgc taagtgtgcg cgcaacggat attgccagtg aacttttatt agaattgagt
    13561 ttgcctctag tgaccttctt tctggcagaa gaagtccatg tttcaggtat tattgccgtc
    13621 gtagttgctg gaattttaaa ggcaagtcgc ttcaagaaaa tcacgctcct cgaagcccaa
    13681 gtggatacgg tgaccgagac ggtctggcat acagtgacct ttatgctcaa cggttctgtc
    13741 tttgtgattt tagggatgga gttggaaatg atagcagaac ctatcttgac caatccaatc
    13801 tataatcctc tacttttatt gctatctctc atcgccctta cctttgtcct ctttgtcatt
    13861 cgttttatta tgatctatgg ctattatgcc tatagaaccc gacgcctcaa gaaaaagcta
    13921 aataagtata tgaaggacat gtttctcttg accttttcag gtgttaaggg aacggtgtcg
    13981 attgctacga ttctcttgat accaagtaat ctagaacagg agtatcctct cttgcttttc
    14041 cttgttgcag gtgtgacgct tgtcagcttt ttaacaggtc tcttggtctt gcctcatctt
    14101 tctgatgaag aggaagaaag caaggattat ctcatgcata tcgccatttt gaatgaagta
    14161 acgctagagt tggaaaaaga gttggaagac accagaaata aacttcccct ctatgcggct
    14221 attgacaatt atcatggacg tattgaaaat ctcattttaa gccaagaaaa ccaggatgat
    14281 caagaagact gggctgcttt gaaactcttg attcttagta ttgaaagtga tggtttggaa
    14341 caggcctatg aagaggggaa cattagcaat cgtgcttacc gagtttacca acgttatctg
    14401 aaaaatatag aacaaggaat caatcgtaaa cttgcctcaa gactgaccta ttattttctt
    14461 gtttccttga ggattttacg ttttctcctt catgaagttt ttactcttgg aaagaccttc
    14521 cgtagctgga aggacaagga gcaaagccgt ctccgtgctc ttgattatga ccaaattgca
    14581 gaactctatc ttgccaatac agagatgatt attgaaagtt tggaaaacct gaagggagtc
    14641 tacagacgct ctttgattag ttttatgcag gagtctcgtc ttcgagaaac agctattatc
    14701 agcagtggtg cctttgtcga acgggttatc aatcgtgtca aacccaacaa tatcaatgaa
    14761 atgctgagag gctattatct ggagcgcaag ttgattttcg aatacgaaga aaaacgatta
    14821 attacaacta agtatgccaa gaaattacga caaaatgtaa ataacttaga gaactattcc
    14881 ttgaaggaag ctgccaatac cctgccgtat gatatggtgg aattggtaag aagaaattag
    14941 ttaatactct ttgaaaatct cttcaaacca cgtcagcgtc gccttaccgt atgtatgtga
    15001 ctgacttcgt cagtcttatc tacaacctca aaacagtgtt ttgagcagcc tgtggctagc
    15061 ttcctagttt gctctttgat tttcattgag tataagatta taagtgaagg agtgtgacat
    15121 gaaaaagtgg tggaaagagc tgatagacaa gcctttatta aaagcttttt tgcattatta
    15181 tcaagcatca gatagtgagt tgaccagtgt cgcagtagcc tactattggt tgatttcgat
    15241 tttccccctg cttttggtgg tggtcaatat cctcccctat tttcagattc ctgtgggaga
    15301 atttctgggc tttatgaaag acgtcctgcc cccaagcctc tatgaaggtg tggaaaaaat
    15361 agccagagaa gtcttgacgc aaccttcaac aggtttattg agtttttctg ttttatcggc
    15421 gttatggagt ttttcaaaat ctatgaattt cttgcaaaaa gcttttaaca aggcttatgg
    15481 cgtcgaaaag agtcgtggac ttatttccca tcagatgcta agtctcctag tcagttttgg
    15541 cttgcagctc ctctttgcgt ttgccttgtt tttgatctta tttggtcaga tgattttggc
    15601 tttacttgct cattattgga caaaagatgg cattatctat caggctttac aagggttggc
    15661 aggtcctctg atttacgccc tcctctttgc tatcttggtc atgctttatt attttcttcc
    15721 caatctatct aacaggaaaa ttagctatac cctaccaggc agtgcctttg ttctcttagt
    15781 gattttaggt ttactaactc tgttctctag ttatctcaat tactatgtcc atcatttggt
    15841 ggatgtccga attttaggtt ccgttcttct tgttgttatg atgttttggt ttatcttgat
    15901 tgctaaaatt gttatcctag gcgcggtgat caatgccagt atgcagagtt tgaaagatcc
    15961 ggtctttaaa aaagactaac tatttatcca ttacaaaagc gcatagtatc aaggctttat
    16021 agaatcttga tactatgcgc ttttgtgctt tgacagtttt ttgtatagtt tttgtccaag
    16081 atttctaaat catctgatta aattcctatt ttcccctatc attgtccctg ttttttcgaa
    16141 aatggaagga gtataataaa tctatcaata aaatgatagg aggaacttta tgggtcatat
    16201 tttctttttt ctaagtgtct ttttggcagg gattctatcc ttcttttctc cttgtatctt
    16261 acctttgtta ccggtctata caggagtgtt actagatgat aaggatggtg ctcaggcttc
    16321 tagcggcaaa ttttcaatct cagttactag tttattacga actctggcct ttatagcagg
    16381 aatttccttt atatttattt tgttgggcta tggagctggt tttttaggcg atttgcttta
    16441 tgcttcttgg ttccaatatc ttactggggc aattattatc cttcttggtt tgcaccaaat
    16501 ggagattcta cactttaagg ggctttataa ggaaaagagg ctacaactgc aaggacaggg
    16561 gcaaaatggt aagggctata gtcaggcatt tttattgggc ttgaccttta gttttgcttg
    16621 gacgccttgc gtggggccgg ttctggggtc tgttttggcc ttggcggctt caggtggttc
    16681 aggagcttgg cagggagctg gtctcatgtt ggtgtatacg ctgggcttgg cgctaccatt
    16741 cttgcttcta gctctgacct ctagttatgt tttgaaacat ttccgaaaac ttcatcccta
    16801 tctcggaatc ctcaaaaaag tgggtggttt tctcattatt gtgatgggct tcttggttct
    16861 gtttggaaat gcttcaattt taagtcaatt atttgaataa aatggaaagg aatatcaata
    16921 tgaaaaaatg gcaaacatgt gttcttggag caggttcgct cctttgtttg acggcttgtt
    16981 caggcaagtc cgtgactagt gaacaccaaa cgaaagatga aatgaagacg gagcagacag
    17041 ctagtaaaac aagcgcagct aaagggaaag aggtggctga ttttgaattg atgggagtag
    17101 atggcaagac ctaccgttta tctgattaca agggcaagaa agtctatctc aaattctggg
    17161 cttcttggtg ttccatctgt ctggctagtc ttccagatac ggatgagatt gctaaagaag
    17221 ctggtgatga ctatgtggtc ttgacagtag tgtcaccagg acataaggga gagcaatctg
    17281 aagcggactt taagaattgg tataagggat tggattataa aaatctccca gtcctagttg
    17341 acccatcagg caaacttttg gaaacttatg gtgtccgttc ttacccaacc caagccttta
    17401 tagacaaaga aggcaagctg gtcaaaacac atccaggatt catggaaaaa gatgcaattt
    17461 tgcaaacttt gaaggaatta gcctaggagg cgtcttatga atgataagtt aaaaatcttc
    17521 ttgttgctag gagtattttt tctagccata accggtttct atgttctatt gatacgaaat
    17581 gcagggcaga cagatgcctc gcaaattgaa aaggcggcag ttagccaagg aggaaaagca
    17641 gtgaaaaaaa cagaaattag taaagacgca gacttgcacg aaatttatct agctggaggt
    17701 tgtttctggg gagtggagga atatttctca cgtgttcccg gggtgacgga tgccgtttca
    17761 ggctatgcaa atggtagagg agaaacaacc aagtacgaat tgattaacca aacaggtcat
    17821 gcagaaaccg tccatgtcac ctatgatgcc aagcaaattt ctctcaagga aatcctgctt
    17881 cactatttcc gcattatcaa tccaaccagc aaaaataaac aaggaaatga tgtggggacc
    17941 cagtaccgta ctggtgttta ttacacagat gacaaggatt tggaagtgat taaccaagtc
    18001 tttgatgagg tggctaagaa atacgatcaa cctctagcag ttgaaaagga aaacttgaag
    18061 aattttgtgg tggctgagga ttaccatcaa gactatctca agaaaaatcc aaatggctac
    18121 tgccatatca atgttaatca ggcggcctat cctgtcattg atgccagcaa atatccaaaa
    18181 ccaagtgatg aggaattgaa aaagaccctg tcacctgagg agtatgcagt tacccaggaa
    18241 aatcaaacag aacgagcttt ctcaaaccgt tactgggata aatttgaatc cggtatctat
    18301 gtggatatag caactgggga acctctcttt tcatcaaaag acaaatttga gtctggttgt
    18361 ggctggccta gttttaccca atccatcagt ccagatgttg tcacctacaa ggaagataag
    18421 tcctacaata tgacgcgtat ggaagtgcgg agccgagtag gagattctca ccttgggcat
    18481 gtctttacgg atggtccaca ggacaagggc ggcttacgtt actgtatcaa tagcctctct
    18541 atccgcttta ttcccaaaga ccaaatggaa gaaaaaggct acgcttattt actagattat
    18601 gttgattaat actcttcgaa aatctcttca aaccacgtca gcgtcgcctt accgtatgta
    18661 gggttactga cttcgtcagt tttatctaca acctcaaagc tatactttga gctgacttcg
    18721 tcagttttat ttacaacctc aaagctatac tttgagctga cttcgtcagt tttatctaca
    18781 acctcaaagc tatactttga gctgacttcg tcagttttat ttacaacctc aaagcggtac
    18841 tttgagaaac ctgtggctat cttcctagtt tactttttga tttttattga gtataagagg
    18901 tctttcctaa gcagttagag gagaattttg ctatactgat actagtaagt ggtaaaggag
    18961 cagagcatga cctacacaat cttaatcgta gaagatgaat atctggtaag acaaggtttg
    19021 actaaactgg tcaatgtagc agcctacgat atggaaatca tcggtcaggc tgaaaatgga
    19081 aggcaggctt gggaattgat ccaaaagcag gtgccagata tcattttaac cgatatcaac
    19141 atgcctcatc taaatggcat ccagttggcc agtctggtac gagaaaccta tcctcaggtt
    19201 catttggtct ttttaacagg ttacgatgat tttgattatg ccttgtctgc tgtcaaacta
    19261 ggtgtggacg actacctgct caaacccttt tctcgtcagg atattgagga aatgttgggg
    19321 aaaatcaaac aaaaactaga caaggaagag aaagaagagc agttacaaga tttattgact
    19381 aataggtttg aaggaaacat ggcccagaaa atccagtctc atctggctga tagccaattt
    19441 agtttaaagt ctttagccag tgacttaggt tttagtccga cctatctgag ttccttgatt
    19501 aagaaagagt tgggcttgcc ttttcaggat tatctggtga gagaacgtgt taaacaagcc
    19561 aagctcttgc ttttaactac agatctgaag atttatgaga tcgcagagaa ggttggtttt
    19621 gaagatatga actattttac ccaacgtttt aagcagattg caggtgtgac acctcgtcag
    19681 tttaagaagg gagaagaccg atgaagcgtt cttctctttt agttagaatg gttatttcca
    19741 tctttctggt ctttctcatt ctcctagctc tggttggaac tttctactat caatcaagtt
    19801 cttcagccat tgaggccacc attgagggca acagccaaac gaccatcagc cagactagcc
    19861 actttattca gtcttatatc aaaaaactag aaaccacctc gactggtttg acccagcaga
    19921 cggatgttct ggcctatgct gagaatccca gtcaagacaa ggtcgaggga atccgagatt
    19981 tgtttttgac catcttgaag tcagataagg acttgaaaac tgttgtgctg gtgaccaaat
    20041 ctggtcaggt catttctaca gatgacagtg tgcagatgaa aacttcctct gatatgatgg
    20101 ctgaggattg gtaccaaaag gccattcatc agggagctat gcctgttttg actccagctc
    20161 gtaaatcaga tagtcagtgg gtcatttctg tcactcaaga acttgttgat gcaaagggag
    20221 ccaatcttgg tgtgcttcgt ttggatattt cttatgaaac tctggaagcc tatctcaatc
    20281 aactccagtt ggggcagcag ggctttgcct tcattatcaa tgaaaaccat gaatttgtct
    20341 accatcctca acacacagtt tatagttcgt ctagcaaaat ggaggctatg aaaccctaca
    20401 tcgatacagg tcagggttat actcctggtc acaaatccta cgtcagtcaa gagaagattg
    20461 caggaactga ttggacggtg cttggcgtgt catcattgga aaagttagac caggttcgga
    20521 gtcagctctt gtggaccttg cttggggcca gtgtcacatc tcttcttgtc tgtctctgct
    20581 tagtgtggtt cagtcttaaa cgctggattg ctcctttgaa ggatttgaga gaaaccatgt
    20641 tggaaattgc ttctggtgct caaaatcttc gtgccaagga agttggtgcc tatgaactga
    20701 gagaagtaac tcgccaattt aatgctatgt tggatcagat tgatcagttg atggtagcta
    20761 ttcgtagcca ggaagaaacg acccgtcagt accaacttca agccctttcg agccagatta
    20821 atccacattt cctctataac actttggaca ccatcatctg gatggctgaa tttcatgata
    20881 gtcagcgagt ggtgcaggtg accaagtcct tggcaaccta tttccgcttg gcgctcaatc
    20941 aaggcaagga cttgatttgt ctctctgacg aaatcaatca tgtccgccag tatctcttta
    21001 tccagaaaca acgctatgga gataagctgg aatacgaaat taatgaaaat gttgcctttg
    21061 ataatttagt cttacccaag ctggtcctac aaccccttgt agaaaatgct ctttaccatg
    21121 gcattaagga aaaggaaggt cagggccata ttaaactttc tgtccagaaa caggattcgg
    21181 gattggtcat ccgtattgag gatgatggcg ttggcttcca agatgctggt gatagtagtc
    21241 aaagtcaact caaacgtggg ggagttggtc ttcaaaatgt cgatcaacgg ctcaaacttc
    21301 attttggagc caattaccat atgaagattg attctagacc ccaaaaaggg acgaaagttg
    21361 aaatatatat aaatagaata gaaactagct aactcccagt caattctggg agttttatgc
    21421 gtaattgggc aaactttcta ggttgtctat cttgctaaaa acaagaaaaa acggtatgat
    21481 agaagagtga caacagaggt aacagagatg aacgagacac agattcaaag agaaacacgt
    21541 caggtagtgg aagatgtttt agaaaagacc aatttgaagc agggggccct ttttgtttta
    21601 ggtctatctt ctagtgaggt gctaggtggt catattggga aagaatccag tcaagagatt
    21661 ggtgagctga tagtggagac tatcttggat attctaggta gtagaggtat ccatttagca
    21721 gttcaaggtt gtgagcatgt caatcgggct ctcgttgttg aacgtcaggt ggcagagcag
    21781 tttggtctgg aaattgtcag tgtccatcct actcttcatg caggaggttc ggggcagttg
    21841 gcagccttta agtttatgca ggatccagtt gaggttgaat ttatcaaggc tcatgctgga
    21901 ttggatatcg gagacactgc aattggcatg catgtcaagc atgttcaggt tccgattcgc
    21961 cctattttga gagagattgg tcatgcccat gtaacggcac tggctagtcg tccaaaatta
    22021 atcggaggtg cgcgtgcgca ctatccgcaa gatgctatta ggaagttttg agaatgaata
    22081 aaggagataa aagtgtttaa aaaagaccgt ttttcaattc gtaagattaa gggagttgta
    22141 ggctctgtat ttcttggaag ccttttgatg gctccttctg tagtggacgc agccacctat
    22201 cactatgtaa ataaagagat tatttcacaa gaagctaaag atttaattca gacaggaaag
    22261 cctgacagga atgaagttgt atatggtttg gtgtatcaaa aagatcagtt gcctcaaaca
    22321 gggacagaag catctgtttt gacagctttt ggtttgctga ctgttgggag cttgctttta
    22381 atctacaaga gaaagaaaat tgctagcgtc tttctagttg gagctatggg attggtagtt
    22441 cttcctagtg caggggctgt agacccagtt gcgaccctag cgctggctag tcgagagggt
    22501 gttgttgaaa tggagggcta tcgctatgtt ggttatctat caggtgacat cctcaaaacg
    22561 cttggcttgg acactgtttt agaagaaacc tcagcaaaac ctggagaggt gactgtggtc
    22621 gaagttgaga ctcctcaatc aataacaaat caggagcaag ctaggacaga aaaccaagta
    22681 gtagagacag aggaagctcc aaaagaagaa gcacctaaaa cagaagaaag tccaaaggaa
    22741 gaaccaaaat cggaggtaaa acctactgac gacacccttc ctaaagtaga agaggggaaa
    22801 gaagattcag cagaaccagc tccagttgaa gaagtaggtg gagaagttga gtcaaaacca
    22861 gaggaaaaag tagcagttaa gccagaaagt caaccatcag acaaaccagc tgaggaatca
    22921 aaagttgaac aagcaggtga accagtcgcg ccaagagaag acgaacaggc accagtcgag
    22981 ccagaaaagc aaccagaagc tcctgaagaa gagaaggctg tagaggaaac accgaaacaa
    23041 gaagagtcaa ctccagatac caaggctgaa gaaactgtag aaccaaaaga ggagactgtt
    23101 aatcaatcta ttgaacaacc aaaagttgaa acgcctgctg tagaaaaaca aacagaacca
    23161 acagaggaac caaaagttga acaagcaggt gaaccagtcg cgccaagaga agacgaacag
    23221 gcaccaacgg caccagttga gccagaaaag caaccagaag ttcctgaaga agagaaggct
    23281 gtagaggaaa caccgaaacc agaagataaa ataaagggta ttggtactaa agaaccagtt
    23341 gataaaagtg agttaaataa tcaaattgat aaagctagtt cagtttctcc tactgattat
    23401 tctacagcaa gttacaatgc tcttggacct gttttagaaa ctgcaaaagg tgtctatgct
    23461 tcagagcctg taaaacagcc tgaggtaaat agcgagacaa ataaacttaa aacggctatt
    23521 gacgctctaa acgttgataa aactgaatta aacaatacga ttgcagatgc aaaaacaaag
    23581 gtaaaagaac attacagtga tagaagttgg caaaacctcc aaactgaagt tacaaaggct
    23641 gaaaaagttg cagctaatac agatgctaaa caaagtgaag ttaacgaagc tgttgaaaaa
    23701 ttaactgcaa ctattgaaaa attggttgaa ttatctgaaa agccaatatt aacattgact
    23761 agtaccgata agaaaatatt ggaacgtgaa gctgttgcta agtatactct agaaaatcaa
    23821 aacaaaacaa aaatcaaatc aatcacagct gaattgaaaa aaggagaaga agttattaat
    23881 actgtagtcc ttacagatga caaggtaaca acagaaacta taagcgctgc atttaagaac
    23941 ctagagtact acaaagaata caccctatct acaactatga tttacgacag aggtaacggt
    24001 gaagaaactg aaactctaga aaatcaaaat attcaattag atcttaaaaa agttgagctt
    24061 aaaaatatta aacgtacaga tttaatcaaa tacgaaaatg gaaaagaaac taatgaatca
    24121 ctgataacaa ctattcctga tgataagagc aattattatt taaaaataac ttcaaataat
    24181 cagaaaacta cattactagc tgttaaaaat atagaagaaa ctacggttaa cggaacacct
    24241 gtatataaag ttacagcaat cgcagacaat ttagtctcta gaactgctga taataaattt
    24301 gaagaagaat acgttcacta tattgaaaaa cctaaagtcc acgaagataa tgtatattat
    24361 aatttcaaag aattagtgga agctattcaa aacgatcctt caaaagaata tcgtctggga
    24421 caatcaatga gcgctagaaa tgttgttcct aatggaaaat catatatcac taaagaattc
    24481 acaggaaaac ttttaagttc tgaaggaaaa caatttgcta ttactgaatt ggaacatcca
    24541 ttatttaatg tgataacaaa cgcaacgata aataatgtga attttgaaaa tgtagagata
    24601 gaacgttctg gtcaagataa tattgcatca ttagccaata ctatgaaagg ttcttcagtt
    24661 attacaaatg tcaaaattac aggcacactt tcaggtcgta ataatgttgc tggatttgta
    24721 aataatatga atgatggaac tcgtattgaa aatgttgctt tctttggcaa actacactct
    24781 acaagtggaa atggctctca tacaggggga attgcaggta caaactatag aggaattgtt
    24841 agaaaagcat atgttgatgc tactattaca ggaaacaaaa cacgcgccag cttgttagtt
    24901 cctaaagtag attatggatt aactctagac catcttattg gtacaaaagc tctcctaact
    24961 gagtcggttg taaaaggtaa aatagatgtt tcaaatccag tagaagttgg agcaatagca
    25021 agtaagactt ggcctgtagg tacggtaagt aattctgtca gctatgctaa gattatccgt
    25081 ggagaggagt tattcggctc taacgacgtt gatgattctg attatgctag tgctcatata
    25141 aaagatttat atgcggtaga gggatattcg tcaggtaata gatcatttag gaaatctaaa
    25201 acatttacta aattaactaa agaacaagct gatgctaaag ttactacttt caatattact
    25261 gctgataaat tagaaagtga tctatctcct cttgcaaaac ttaatgaaga aaaagcctat
    25321 tctagtattc aagattataa cgctgaatat aaccaagcct ataaaaatct tgaaaaatta
    25381 ataccattct
//
LOCUS       DAVHEX010000031        24795 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVHEX010000031 DAVHEX010000000
VERSION     DAVHEX010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24795
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.031"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(246..1145)
                     /gene="galU"
                     /locus_tag="VVL45_001207"
     CDS             complement(246..1145)
                     /gene="galU"
                     /locus_tag="VVL45_001207"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEU4187386.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDKTTDMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FEILEKQAPGAGNEIQLTDAIDTLNKTQRVFAREFKGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(1167..2183)
                     /locus_tag="VVL45_001208"
     CDS             complement(1167..2183)
                     /locus_tag="VVL45_001208"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU4187387.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKDVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQKLFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            2395..2775
                     /locus_tag="VVL45_001209"
                     /pseudo
     CDS             2395..2775
                     /locus_tag="VVL45_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809823.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            complement(2949..3374)
                     /locus_tag="VVL45_001210"
     CDS             complement(2949..3374)
                     /locus_tag="VVL45_001210"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU4187388.1"
                     /translation="MTNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKSLTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTCLTE"
     gene            complement(3442..4092)
                     /gene="phoU"
                     /locus_tag="VVL45_001211"
     CDS             complement(3442..4092)
                     /gene="phoU"
                     /locus_tag="VVL45_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU4187389.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(4107..4859)
                     /gene="pstB"
                     /locus_tag="VVL45_001212"
     CDS             complement(4107..4859)
                     /gene="pstB"
                     /locus_tag="VVL45_001212"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU4187390.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDILLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(4861..5676)
                     /gene="pstA"
                     /locus_tag="VVL45_001213"
     CDS             complement(4861..5676)
                     /gene="pstA"
                     /locus_tag="VVL45_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU4187391.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(5669..6532)
                     /gene="pstC"
                     /locus_tag="VVL45_001214"
     CDS             complement(5669..6532)
                     /gene="pstC"
                     /locus_tag="VVL45_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU4187392.1"
                     /translation="MTKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFIANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRSFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(6650..7525)
                     /locus_tag="VVL45_001215"
     CDS             complement(6650..7525)
                     /locus_tag="VVL45_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HEU4187393.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(7692..9023)
                     /locus_tag="VVL45_001216"
     CDS             complement(7692..9023)
                     /locus_tag="VVL45_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEU4187394.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGLGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSGLKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(9024..9731)
                     /locus_tag="VVL45_001217"
     CDS             complement(9024..9731)
                     /locus_tag="VVL45_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4187395.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(9815..10243)
                     /locus_tag="VVL45_001218"
     CDS             complement(9815..10243)
                     /locus_tag="VVL45_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU4187396.1"
                     /translation="MAPTDKEDLIRTLELAPHIEGGYFRQTKKASDTINLAGKGRALY
                     TSIYFLLEETNPSHFHRLTADGHYEAVTLGLDISKGQQLHYCVPKGTIWGSTVDKDDA
                     LVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTRY"
     gene            10229..10786
                     /locus_tag="VVL45_001219"
                     /pseudo
     CDS             10229..10786
                     /locus_tag="VVL45_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(11101..12792)
                     /locus_tag="VVL45_001220"
     CDS             complement(11101..12792)
                     /locus_tag="VVL45_001220"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEU4187397.1"
                     /translation="MNTKELIASELSSIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGIGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            12928..13374
                     /gene="argR"
                     /locus_tag="VVL45_001221"
     CDS             12928..13374
                     /gene="argR"
                     /locus_tag="VVL45_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU4187398.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            13425..15959
                     /gene="mutS"
                     /locus_tag="VVL45_001222"
     CDS             13425..15959
                     /gene="mutS"
                     /locus_tag="VVL45_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEU4187399.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLVTGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            16106..17800
                     /gene="patA"
                     /locus_tag="VVL45_001223"
     CDS             16106..17800
                     /gene="patA"
                     /locus_tag="VVL45_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HEU4187400.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLGALLTG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQDGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
     gene            complement(18131..18400)
                     /locus_tag="VVL45_001224"
                     /pseudo
     CDS             complement(18131..18400)
                     /locus_tag="VVL45_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            18592..20358
                     /gene="patB"
                     /locus_tag="VVL45_001225"
     CDS             18592..20358
                     /gene="patB"
                     /locus_tag="VVL45_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HEU4187401.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIVFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     SQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            20497..21186
                     /locus_tag="VVL45_001226"
     CDS             20497..21186
                     /locus_tag="VVL45_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU4187402.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            21190..21369
                     /locus_tag="VVL45_001227"
     CDS             21190..21369
                     /locus_tag="VVL45_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187403.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            21356..22705
                     /locus_tag="VVL45_001228"
     CDS             21356..22705
                     /locus_tag="VVL45_001228"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEU4187404.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAAVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            22829..24289
                     /locus_tag="VVL45_001229"
     CDS             22829..24289
                     /locus_tag="VVL45_001229"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEU4187405.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREEFIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDEFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENMLKEISK"
     gene            24303..24737
                     /locus_tag="VVL45_001230"
     CDS             24303..24737
                     /locus_tag="VVL45_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU4187406.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAGDGQIFTGF
                     CMEGTCGVFHLCAERAALFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGKERASKFNER"
BASE COUNT         7366 a         4857 c         5297 g         7275 t
ORIGIN      
        1 ttattatttt ggaaccatta gaaacaacat agcaaagtta aaataaggct ttgtccgtac
       61 acaacttgaa aaagtgcgca ccgattcggt gcttttttgt tttactatac taatttattc
      121 tactcttatt ttaatgaaaa aacaaataca agtatagcgt tttcattagt ttttattaaa
      181 taggtagatt caagtagaga atttacttaa tttatgtgtg gctaatcttt atataaatga
      241 ttttgttatt ccttttcagt caattctttt ccaagttgga tgaggtaatt cttcaaatca
      301 tctttgactt gtgggtgttt gagggcgtag tcgatggatg ttttcatgaa gccaaacttg
      361 tctccgacat cgtaacgagc ccctttgaac tcacgagcaa atacacgttg tgttttattg
      421 agggtgtcga ttgcatctgt cagctgaatt tcatttcctg cacctggagc ttgcttttcg
      481 agaatctcaa aaatttcagg cgtgaggagg tagcgtccga taatagcaag gtcgctagga
      541 gcgtcctctg gagctggttt ttcaacaaag gtttcaacac tgtaaagacc atcttttcct
      601 tcgccttgcg gagcaataac cccgtaagca gatacttcgt catgagggac tggcatgaca
      661 gcgatagtag acgcgtgggt acgctcgtag tcatccatga gttgtttggt aagtggaaca
      721 gccttttcgt ctgtgatatc catcaagtca tcaccaagca taacgacaaa aggttcattt
      781 ccgacgaaag ccttggcttg caaaacagca tctccgagac cgcgtggatg agtttggcgg
      841 ataaaatgca gacgcatgtc agttgtttta tcaactagct tcaaaagatc tgttttccct
      901 ttttctttga ggttatattc caattcgaag tttgaatcaa agtggtcctc aatagaacgt
      961 tttgatttac cagtgacaac tagaatatct tcaatacctg atttgagagc ttcttccacg
     1021 ataaactgga tagttggttt gtctacgatt ggcaacattt ctttggcaag ggccttggtt
     1081 gctggtaaaa atcgagttcc tagtccagca gcagggatga ctgcctttct aacttttgat
     1141 gtcataaaaa tcctttctat agagggttaa gaccactcat tttctgcttt aaattcattg
     1201 ttcatgatgt cataaatggc atctttgata ttggttccgt gataaataac ttggtaaata
     1261 gcctgtgtaa tgggcatata gactccaagt tcttgggcta gttcataggc tgctcgagtc
     1321 gttgaaattc cttcgattac catgcccata ttagcttcta tatcagctag ggattctcct
     1381 cgtccgagag catctccagc tctccagtta cgagagtgga tggaagttcc cgttacgatc
     1441 aaatctccca caccagataa gccgctatag gtcaatggac tggccccgag tgctacccct
     1501 aggcgggtga tttctgctaa acctcgagcg atgatggctg ccttagcatt atcaccaaat
     1561 ccaagaccat gtaaagctcc agcaccgaca gcaataatat ttttaagagc accagcagtt
     1621 tcaaccccga taacatccgt attggtataa agtcggaagt agtgattact aaatagcttc
     1681 tgaacgtatt gagctgtttg taaatcttta gaagcagcag ttattaaagt taggtcacgc
     1741 acaatggtct cttctgcatg actaggccct gaaacaacga cgatatcact acggagatgt
     1801 tcaggaattt cttcttcaag aatggttgat aatcgtttat ggctatcagg ttctaatccc
     1861 tttgatgcgt gcatgatgat aaccttatgg tccaaggttt gtgcaacttg ctgggcaaca
     1921 agtcgtgtca cttttgttgg gacaacaaac aaaatcgcat ccacatcttt caatgtttct
     1981 gctaagtcgg tgtaggcaat gatattttcg tctagaacga catctttaaa gtagtgctta
     2041 ttagtatggt gtgtattaat ttcattgatt tgctcgggaa gatttcccca aatacgtacc
     2101 tcgtgtccat tgtcatttaa gacttgtgaa agggcggttc cccaagaacc aggccccaag
     2161 acggcgacgg tttgtttttc catgatttcc tccttgatag agttctttct cttattctat
     2221 catattctag gggattttgc acttgcatta ctggggtatg gtggaaaaat acacaaaaac
     2281 cgagacgaag ggataaactc ttagtcttta gttaaagtga gaagaaatct gatagatagt
     2341 ttgattcagt atatgcttat aatagaggta aacaactcag gaagttcttg taggttgcga
     2401 gagtttggcg aaaaaattaa aagattacgt ttggctaaaa aatcagtcgt tcagaatttt
     2461 gtggtgatga gtctgaatta agtatccgtc aattaattag aattgaaaat ggagaatcca
     2521 gaccaatact aacaaagtta aaatatattg ctgaacgttt gggggttgaa gattacaagt
     2581 tgatgccaag ttatatagag ttggataagg aatacctaga attgaagtat ttcttgatga
     2641 ggactcctac atacgaagat gaaactatcg cccaaaagaa agagagtgtt tttgataaga
     2701 tttttgaaga gtattatgat aggctacctg aggaagaaag atttatcatc ccaaattatt
     2761 catatctggc actaacgaac tacacagttc aaaaattacc agaaaagcta gttgaaatac
     2821 tgtccttctg gtgattttgc ttaatttctg atgttcgaga cataattgag gagatttgtc
     2881 ttgatttttt cctaaaaaag ggcagactcc tcccttggtt tgatgctcga tttttaaaat
     2941 aaggtgactt attctgttag gcatgttttc ccctgccctc tagctctata tggaacaggg
     3001 agtatcagat ttatctggca gattgtatca aataaggctt gaaactgttt tgaataggcg
     3061 tatagacttg agaatttgag aatatgtcgt ctagcagtag agacatattt tccggcaata
     3121 tgaaggaaga gacgtcgaaa acgcttgata gtcaaggact ttacttcatc accagctagc
     3181 tgctttaaaa agaggtagag attataagcc agacagccca tcatcatacg tacttcattc
     3241 ttaatcatgg tcgaactatc tgtcttatcc ccaaagaatc ctgctttcct ttctttgata
     3301 aagttttcag cattccctcg ttcacgatag aattgaaaca actcctcact atctacactg
     3361 cttaaattgg tcaaatgaac ttttttacaa ccataaaaaa gagtacttaa tcgtactctt
     3421 ctcttttaag gattagttga attaattcaa atccactagt tctcctgttt ctaggtagac
     3481 taggcgttca caaatgttag caatgtaatc agcgaagcgt tccagatgcc ctatgatata
     3541 aaggtattga gttccattgg gaattgaggt ttcttggtct ttcataagtc caatgatttc
     3601 ctttgataag gcataataat attggtcaat ctgttcatct ttttgagcaa tactaatagc
     3661 ttttgaggct tggtgcaaag gaaaggcaac caataaatcg gctagcatgc tgagggataa
     3721 tttacccatt tggtgtaact gttcttcgtc aggggctagt tgattttctt ttagttgcaa
     3781 aacagctttg gcaatgcctg ccatatggtc tcccatacgt tcaaggtctg aacaagaaga
     3841 catgatgcta atcacaaatc gaaggtcaga cacttgtggc tgctgcaagg ccaacaaacg
     3901 ggcacaggtc aattcgatag cgctttgacc ttggttgata gcatgatcct tattgataat
     3961 tagctctgcc atctccttgt ctttggaggc taaggccagt aaggcttttg aagctgtttc
     4021 aaggacaagt tgccctagtc ctaaaaagga ttgttctaat tcatgcaatt ctaagtcaaa
     4081 ttgatttctc ataggttttt ccttccttat ccgaaccgtc ctgaaatata gtcttctgtg
     4141 cgctgatctt ttggattggt aaacacgtca acggtatctc caaattcgca aatttctcct
     4201 gttaagaaaa aagcagtttt atctgaaata cgtgaagctt gttgcatgtt atgggtaaca
     4261 atgataatcg tataatcctt ttttagttgc tgaatgaggt cttcaatttt taaagtggag
     4321 atagggtcta aggctgaagt cggctcatcc attaacagaa tatcaggttc tactgctaaa
     4381 gctcgcgcaa tgcaaaggcg ttgctgctga ccgccagata aggacatggc actcttttta
     4441 agatcatctt tgacttcttc ccaaatggct gcccctttta aagatttctc cactaaggca
     4501 tctaattgtt ttttgtctcg aataccatgt gtccttgggc cataagccac gttatcatag
     4561 atagacatgg caaagggatt aggctgttga aaaaccatcc ctacacgctt acgtagctga
     4621 ttaaggttga atttgctact ataaatatct tgctcatcta agaggacttg gccttcaata
     4681 tggcaagaag gaaccaaatc gttcatccgg ttaagggttt ttagaaaagt tgatttgcca
     4741 caaccagatg ggcctatcaa ggcagtaatc tgtctttctg gtaattgaat cgaaatattt
     4801 tttaaggctt gaaaatcccc gtaaaataag tctaggtgtc tgactgaaaa tgttcccata
     4861 ctaggaagct cctttcacaa gttttcgaga taataagctt gatagagtat ttatcattaa
     4921 aacagtaata atcaaaatca cgccggtagc ataggcttca ttgacatgta gcccctcact
     4981 tgacagcata tacatatgta gggctagaga acggcctgaa gacatgagac tacttggcgt
     5041 attggtagag gtacctaatg tatacatgag ggcagctgtt tcaccaacga tacggccaat
     5101 agctagtatc actccagcta aaatacctgg catggcaact ggtagaacaa ttctaaaaac
     5161 agtccgtaac ttacctgccc caagtccata acttgcttga cgcatgctat cactaacaga
     5221 taaaagggct tcttctgttg agcgaataat gactggcaac accatgataa ctgaggttaa
     5281 gattcctgat aacagagagt attgaaaacc taagaagact acaaagaaga gcatgccaaa
     5341 cagaccaaaa acaatggaag gaatcccaga taaggtatct gaggccaatc gcatgatttt
     5401 aacacaaagg gaatcttttt ttgtatattc cacaagataa aaaccagcaa aaatccctat
     5461 gggcaaggct aaaagaagag caccaaagac cagaataacg gtggaaataa tcgctggcat
     5521 aagggaaatg ttctcagaag tataagtcca agaaaagagg gatagactta gatgaggtaa
     5581 gcctttgatg aggataaaac caatgattaa aaagagagag ccaaaggtta aagctgaaaa
     5641 acaataaacg agaagtttta gcaggtattt actcataaga tgattttcct ttcaagtagg
     5701 caaagtaggc attaatcaag agaataagga aaaagagaac tgctgaggtt gcaataaggg
     5761 cttccctatg ctgacctgat gcgtaagcca tttccagaac aatattggtt gttaaggttc
     5821 tggttcctga aaagagtcca cttggaataa tcggctggtt gcctgccacc aaaataactg
     5881 ccatggtttc acctactgcg cgaccgattc ctaaaataac tgctgataaa ataccagatc
     5941 tcgcagctgg caagatgaca ctaaaaatac tccgttcatg actagctcct agagccaagc
     6001 taccagaata atacgttttg ggaactgttc ggatagcaga ttctgacaaa ctgataatgg
     6061 ttggcaaaat cattattcct aatagtaacg aagcggttag gacactcatg ccatttccta
     6121 aaaagcttct aatccaaggc accaataatt gtaggccgaa aaaaccataa acaatagatg
     6181 gaatggctgc catcaagttg atagctgatt ttaagaagcc atagacgggc tttggacaat
     6241 aataaaccat aaacaccgat gtcaagatgc ctgttggcac cccaatcaca atcgctccta
     6301 aggtaattaa taaggaacca acgatcattg gtaaaatacc atagcttgcc ggaatgttcg
     6361 ttggcgacca atcactgcct aataaaaaac gggcaaagcc gtagttagct atgaaaggta
     6421 agccattact aaaaataaag aaacagatta gcaaaatagc tacaacagct actgttgcac
     6481 tcatgaaaaa aattgcccta aaaactgctt ctttgaaggc ttgttttgtc acatcttgtc
     6541 ctttctagtg aagaaagtaa gggagatacg acacctccct acttgccttc tttatcttat
     6601 tgtacgatga aacgtctgca tctctttaga gatttatgga gcaaacattt tatttaatct
     6661 tgtcccaggt ggttaatttg ccactaaaaa cgtctgcaag ttcagccata ctgacttggc
     6721 ttgccttatt gtcattattg accacaacag caataccgtc taaagcaata gcatcatggg
     6781 tgagactctt accttcttca ggagttaatt ccctagaaac cataccaata tcagcggttt
     6841 tctccttaac agcggtaata cctgctgaag acccattaga ggtaatatca atcgtaactt
     6901 ctggattttc ttttttataa gcttctgcta atttttccat taaagaagat actgaagtgg
     6961 aacctacaac agacaacttg cctgataagt gttggcttgt atattccgtg gtttcggttt
     7021 tagcttcaat aaatttatta tctgtgacca cttgttgacc ttgtttggag tggataaagc
     7081 tgataaaatc ttgacctagc ttggaaagat tagaagacca aacaatgttg aagggacgtt
     7141 gaagagggta ttcaccatct aaaactgtgt ctcgactagc cttgacacca tcaatctcta
     7201 aagccttgac agatttcgtt aaagatccca aggagatgta gccgatagca ttagcattcc
     7261 cttgaactgc tgagagaaca ccttctgtac tattttgaat cacagctgtt ttggcagtgt
     7321 tgtcaatttt tttatcaccg tcttttttga gaatccctgt gatttctgtg aaggcacccc
     7381 gtgtcccaga gccattttct cgtgaaatca cctcaatcgt tcctgaagct gactgtttgg
     7441 aagcagctga ctgattgcca caggcaacaa gcccaaatcc tgataagcca atggctgcaa
     7501 gagtaagcat ttttttgaat ttcataataa tcacctttat ctctatgtat ttttcttgtg
     7561 taggcttact acatttatag tctaacaagt ctttgtaaag gtttatccct gattcatgta
     7621 aagattgtgt aaagaatcaa aaaaagccac ttttgaaaaa tggctgctcc taaaaatagc
     7681 tttaaaaatt attagtcctg tgcgaaagat tggttaggaa gaaaaatcgt gaagcaactg
     7741 cctctgccaa gctgactcgt caccgtgact tggccaccta ataattgact gagttctttg
     7801 acaatggcaa ggccaagacc agtgccacca gtttgtctgc ttcgaccttt attaactcgg
     7861 taaaaacgtt caaaaatacg atcctgctct aattgactaa taccaatccc tgtatctgat
     7921 acagaaatct taatgccttc gttcaccttt tgggtcttga cctcaatttt tcccccttgt
     7981 tcagtgtaac ggatggcatt ggataaaaga ttgagtaaga tttgggaaag taattgacta
     8041 tctgatacga gggtgacatc atctggcacc tgcaccttta gctgtaaatc cttcttcttg
     8101 agctgaggtt gcaagctttg agtcaaatcc tgtacaaatt ctgccaaaga aagggtcgtc
     8161 cattgtatag gcatttgttg agccttagat aaggtaagaa gatgctcaac aatatgctca
     8221 agacgcaaac tttctttgta aataatgtct agaaagtcat ccttgagcgc ttcttcttca
     8281 gctgacatcc ccttaatggt ttcagcaaag cccttaatcg aagtaactgg tgtcctcaat
     8341 tcatgggagg catttgagac aaaggctaaa tttaactttt cataagttct aatcgttgtt
     8401 aaatcatata gcaagacgag cacagcttcc acagattggg tggggctaaa aacgggaact
     8461 gctgtcactt ctaaaatcaa gtcaccctca tgaaacccac ttacttcttg ttttaacctt
     8521 gttttttgat caaaggcttg gtgaactaaa ttccgaatat ccatccgttt gaggtcatca
     8581 agtgaactta tgtcgccgtc cacatcggga aaataatgag gcagagagcg actggataat
     8641 aacatctgac cttgagcgga aactaaaaac gtccccatgg ttaggtgcga cagaagaacc
     8701 tccattgttt cggctagatc cttgtattgc tgatcctgtt gggagacttt ggtttttagg
     8761 ccagacacat actgagccaa agactttaag tcttcttgcc ctttttctaa aaagtattca
     8821 ctactggtca agagaggttg gtgcaaggtc tcaaaagcaa cttcccattt ccaaaggcaa
     8881 aagagccagt agccacctag tcccaaagaa agggctagaa gaaagagacc gatgccttta
     8941 ctgatccaag ttaatgccat ccctgcaatc agaatgaggc taacacttag attgactagc
     9001 caaaattgaa ggtagcgttt catctataac tccttgaact tataaccata accccgaatg
     9061 gttcgaataa attgaggggc tttaggattg tcttcaattt tttccctcaa cttaccaata
     9121 tgaacgtcca ccaaacgtgt ttcctgccca aagtcatacc cccagatacg ttccaaaaga
     9181 cgctctctag tcagtgtcat gttgggatgt ttcataagat agagcaagag ttcaaattct
     9241 tttggggtca aactcagtaa cttattcgcc ttgtagactt catgacgctc agggtatact
     9301 ttcaaggtcc caaatagcca agaatcgtca gcgatattat ctgaatcatc tccttcttgt
     9361 tctcctttag ttcgcctgag gacagccttg acacgcgcca gcaattctct agggctaaaa
     9421 ggcttggtca ggtagtcatc agcccctaat tccaaggcca aaaccttatc aaattcatca
     9481 cttttcgcag aaaccatcat aattggagtt ttgacgcctt tggctctcag ccgcttacaa
     9541 acttccatgc catctaattg tggtaacatg atatcaagca agataaaatc aaagggttct
     9601 gtttctgcca aagctaaggc cttccgtcca tttgtcacca attgagtaga aaagccttcc
     9661 ttacttaaat ggtagtcaag caatttcaga atgtgttctt catcatccac taataagact
     9721 tgttttgtca tctattatct cctattggta acattataac acaattatca gaaatcctaa
     9781 cattgctaaa tcagattaaa tttgcctatc aagactagta tctggtcaaa cgctcaatca
     9841 tctccttgtg ctctggatag gtcgccagta gatctaccct ttcaaataat tcaaaatcct
     9901 caaattcaaa accaggagca acaagacaag aaaccagagc atcatcctta tcaactgttg
     9961 atccccaaat agtgccctta ggaacacagt agtgaagttg ttgccctttg gatatgtcca
    10021 ggcctaaagt gactgcttcg tagtgaccat ctgctgtcaa tcggtgaaaa tgtgaaggat
    10081 tcgtttcttc taataagaaa taaatactgg tataaagcgc ccttccctta ccagcaaggt
    10141 ttatagtgtc tgaagctttt tttgtttgtc taaaatagcc accttcaata tggggagcta
    10201 actctagagt tcttatcaag tcttctttat ccgtcggagc caatgggttg aagtaactct
    10261 tgttcaaagt ggttttacga tttcaagaac tcctctcagt tctgaggaca cggtaatgat
    10321 tgatgcgacg gaagtacaaa tcaatcgccc taaaaaaaga attagcgaat gattctggta
    10381 aaaaaaatgc cacgctatga aggctcaagc gattgtcaca agtcaaggga gaattgtttc
    10441 tttggatatc gctgtgaact attgtcatga tatgaagttg ttcaaaatga gtcgcagaaa
    10501 tatcggacaa gctggtaaaa tcttggctga cagtggttat caagggctca tgaagatata
    10561 tcctcaagca caaactccac gtaaatccag caaactcaag ccactaacag ttgaagataa
    10621 agcctataac catgcgctat ccaaggagag aagcaaggtt gagaacatct ttgccaaagt
    10681 aaaaacgttt aaaatgattt caacaaccta tcgaaatcat cgtaaacact tcggattacg
    10741 aatgaatttg attgctggca ttatcaatca tgaactagga ttctagtttt gcaggaagtc
    10801 tattatttgg ttaggtgaat tagtgaagcg tttaggcaag tgtctctggt tacgacgtca
    10861 tggactctaa atcgattata tttaggggtc atgactagtg aagcagttag ctagttcgca
    10921 tataagcggc tagcgtctaa caattaggaa ctttagttcc aataacttta agattacgac
    10981 gttttaggac ataaatcgat catatttatg tcctaaaact agtgaagcgc ctagccaaag
    11041 tccgaatagg atttggcgtt agttacttag attgctttgc aatcaagtaa ctttggcgat
    11101 ttacatcttc tctggcgctt ctactccaag caagcgaagg gcttctttga gaacgactgc
    11161 ggttgcgtag ctgagggcta gacggctgtc gcgttctggg ctttcatcca agatacgtgt
    11221 atgtgcatag tatttgttaa aggattgagc caggctaatt gcaaatttag caatgataga
    11281 aggttcaaag ttatctgccg cacggttgat aatacgtggg aagtcttgaa tgagtttaat
    11341 gatttcccag ctttcagtat cattcaagct atagttgcca gctgtttctg gtttgaaatc
    11401 ggctttgcgt aagatagatt ggatacgagc gtaggcatat tgaacgtaag gtccagtttc
    11461 accctcgaag gataccatag cctctaggtc gaagtcgtat ccatttgtac ggtcggtttt
    11521 gaggtcatag aatttaatgg ctccaatccc aacagcatgt gctacttggt ctttgttttc
    11581 tagttcagga tttttagcct cgatttggac cttggcacgg ctaacagcct ctgcaacagt
    11641 aggctctagc aagatgacat tccctttacg agtagagagt ttcttccctt cttttgtaac
    11701 caaaccaaaa ggaacgtgag taatgtcgtc actccagtcg tagcccatct cttgcaagac
    11761 agctttgagc tgtttaaagt gggcagattg ttcttgacca acgacataga tagatttagc
    11821 aaattggtat tcgtttttac ggtagagggc tgcagccaag tcacgtgtga tatagagagt
    11881 tgcaccatca gacttcttga tgagggctgg atgttcaatt ccatatttct caagattcac
    11941 aacttgggca ccttctgatt caagaagtag tcctttttca gaaagaatgt ctacaactgc
    12001 atccatctta tcattgtaga aggcttctcc gttatagctg tcaaattcaa ccttcaattc
    12061 attgtaaagg cggttaaatt ccactaaact ttcatcgcgg aaccattgcc aaagagcgag
    12121 agcttcctca tctccatttt caagtttacg gaaccattcg cgcgcttctt catccaagct
    12181 agggtcattt tcagcttcag cgttgatgcg gacatagagt ttaaggagtt catcgattgg
    12241 atgagctttt acagcttctt cgtcgcccca ttttttgtag gcaacaatca acatcccaaa
    12301 ttgtttaccc cagtctccca aatggttgac cttgaccgtt tgataaccga ttttttggaa
    12361 aatatgtgac aagctatctc cgataacagt tgaacgcagg tggccaatag aaaatggttt
    12421 agcgatattc ggactagaca tgtcgataac aacattttct tgtttaccaa tattttggtc
    12481 agcatagtgt tctttttcag tggtaacagc ttgcaatact tgagcagaaa tggcagattt
    12541 atcaaggaaa aagttaacgt aaggtcctgt tgcgacaact ttttcaaagg cttggctgtt
    12601 cattttttca gccagttcag ccgcaatcat ttgtggtgct ttacgttcga cttttgcaag
    12661 agaaaaagca gggaaagcaa tgtctcccat ttctgagttt ttaggggttt ccagtaactt
    12721 taaaatagcc tcttggtcca ggctatcaat gatgctagat aattcgctag caatcaattc
    12781 ttttgtattc attaagagct cctttttgga cttttctact attttatcac aattttaaag
    12841 aaagaagaaa aaatttttga aatctcctgt ttttttggta taatatggtt ataaatatag
    12901 ttataaatat gcacgcaaga ggattttatg agaaaaagag atcgtcatca gttaataaaa
    12961 aaaatgatta ctgaggagaa attaagtaca caaaaagaaa ttcaagatcg gttggaggcg
    13021 cacaatgttt gtgtgacgca gacaaccttg tctcgtgatt tgcgcgaaat cggcttgacc
    13081 aaggtcaaga aaaatgatat ggtgtattat gtactagtaa atgagacaga aaagattgat
    13141 ttggtggaat ttttgtctca tcatttagaa ggtgttgcaa gagcagagtt taccttggtg
    13201 cttcatacca aattgggaga agcctctgtt ttggcaaata ttgtagatgt aaacaaggat
    13261 gaatggattt taggaacagt tgctggtgcc aataccttat tggttatttg tcgagatcag
    13321 cacgttgcca aactcatgga agatcgtttg ctagatttga tgaaagataa gtaaggtctt
    13381 gggagttgct ctcaagactt atttttgaaa aggagagaca gaaaatggcg atagaaaagt
    13441 tatcacccgg catgcaacag tatgtggata ttaaaaagca atatccagat gcttttttgc
    13501 tctttcggat gggtgatttt tatgaattat tttatgagga tgcggtcaat gctgcgcaga
    13561 ttctggaaat ttccttaacg agtcgcaaca agaatgccga caatccgatc cctatggcgg
    13621 gtgttcccta tcattctgcc caacagtata tcgatgtctt gattgagcag ggttataagg
    13681 tggctatcgc agagcagatg gaagatccta aacaagcagt tggggttgtt aaacgagagg
    13741 ttgttcaggt cattacgcca gggacagtgg tcgatagcag taagccggac agtcagaata
    13801 attttttggt ttccatagac cgcgaaggca atcaatttgg cctagcttat atggatttgg
    13861 tgacgggtga cttttatgtg acaggtcttt tggatttcac gctggtttgt ggggaaatcc
    13921 gtaacctcaa ggctcgagaa gtggtgttgg gttatgactt gtctgaggaa gaagaacaaa
    13981 tcctcagccg ccagatgaat ctggtactct cttatgaaaa agaaagcttt gaagaccttc
    14041 atttattgga tttgcgattg gcaacggtgg agcaaacggc atctagtaag ctgctccagt
    14101 atgttcatcg gactcagatg agggaattga accacctcaa acctgttatc cgctacgaaa
    14161 ttaaggattt cttgcagatg gattatgcga ccaaggctag tctggatttg gttgagaatg
    14221 ctcgctcagg taagaaacaa ggcagtcttt tctggctttt ggatgaaacc aaaacggcta
    14281 tggggatgcg tctcttgcgt tcttggattc atcgcccctt gattgataag gaacgaatcg
    14341 tccaacgtca agaagtagtg caggtctttc tcgaccattt ctttgagcgt agtgacttga
    14401 cagacagtct caagggtgtt tatgacattg agcgcttggc tagtcgtgtt tcttttggca
    14461 aaaccaatcc aaaggatctc ttgcagttgg cgactacctt gtctagtgtg ccacggattc
    14521 gtgcgatttt agaagggatg gagcaaccta ctctagccta tctcatcgca caactggatg
    14581 caatccctga gttggagagt ttgattagcg cagcgattgc tcctgaagct cctcatgtga
    14641 ttacagatgg gggaattatc cggactggat ttgatgagac tttagacaag tatcgttgcg
    14701 ttctcagaga agggactagc tggattgctg agattgaggc taaggagcga gaaaactctg
    14761 gtatcagcac gctcaagatt gactacaata aaaaggatgg ctactatttt catgtgacca
    14821 attcgcaact aggaaatgtg ccagctcact ttttccgcaa ggcgacgctg aaaaactcag
    14881 aacgctttgg aaccgaagaa ttagcccgta tcgagggaga tatgcttgag gcgcgtgaga
    14941 agtcagccaa cctcgaatac gaaatattta tgcgcattcg tgaagaggtc ggcaagtaca
    15001 tccagcgttt acaagctcta gcccaaggaa ttgcgacggt tgatgtctta cagagtctgg
    15061 cggttgtggc tgaaacccag catttgattc gacctgagtt tggtgacgat tcacaaattg
    15121 atatccggaa agggcgccat gctgtcgttg aaaaggttat gggggctcag acctatattc
    15181 caaatacgat tcagatggca gaagatacca gtattcaact ggttacaggg ccaaacatga
    15241 gtgggaagtc tacctatatg cgtcagttag ccatgacggc ggttatggcc cagctgggtt
    15301 cctatgttcc tgctgaaagc gcccatttac cgatttttga tgcgattttt acccgtatcg
    15361 gagcagcaga tgacttggtt tcgggtcagt caacctttat ggtggagatg atggaggcca
    15421 ataatgccat ttcgcatgcg accaagaact ctctcattct ctttgatgaa ttgggacgtg
    15481 gaactgcaac ttatgacggg atggctcttg ctcagtccat catcgaatat atccatgagc
    15541 acatcggagc taagaccctc tttgcgaccc actaccatga gttgactagt ctggagtcta
    15601 gtttacaaca cttggtcaat gtccacgtgg caactttgga gcaggatggg caggtcacct
    15661 tccttcacaa gattgaaccg ggaccagctg ataaatccta cggtatccat gttgccaaga
    15721 ttgctggctt gccagcagac cttttagcaa gggcggataa gattttgact cagctagaga
    15781 atcaaggaac agagagtcct cctcccatga gacaaactag tgctgtcact gaacagattt
    15841 cactctttga tagggcagaa gagcatccta tcctagcaga attagctaaa ctggatgtgt
    15901 ataatatgac acctatgcag gttatgaatg tcttagtaga gttaaaacag aaactataaa
    15961 accaagactc actagttaat ctagctgtat caaggagact tctttgacaa ttctccactt
    16021 ttttgctaga ataacatcac acaaacagaa tgaaaaggag ctgacgcatt gtcgctccct
    16081 tttgtctatt ttttaaggag aaagtatgct gattcagaaa ataaaaacct acaagtggca
    16141 ggccctggct tcgctcctga tgacaggctt gatggttgct agttcacttc tgcaaccgcg
    16201 ttatctgcag gaagtcttag gcgccctcct tactgggaaa tatgaagcta tttatagtat
    16261 cggggcttgg ttgattggtg tggccgtagt cggtctagtt gctggtggac tcaatgttgt
    16321 cctcgcagcc tatattgccc aaggagtttc atccgacctt cgggaggatg ccttccgtaa
    16381 aattcaaacc ttttcttatg ctgatattga acaatttaat gcgggaaatc tagtcgttcg
    16441 aatgacaaat gatatcaacc agattcagaa cgttgtcatg atgaccttcc aaattctttt
    16501 cagacttccc ctcttgttca tcggttcgtt tatcctagcg gttcaaacct taccttctct
    16561 gtggtgggtg attgttctca tggtagtctt gatttttggt ttgactgctg tcatgatggg
    16621 aatgatgggg cctcgttttg ccaagtttca aacccttctt gagcgcatca atgccattgc
    16681 caaggaaaat ttacgtggcg ttcgtgtggt caagtccttt gtccaagaaa aagagcaatt
    16741 tgctaagttt acagaggtct cagacgagct tcttggtcaa aacctttaca ttggttatgc
    16801 cttttcagta gtggaaccct ttatgatgtt ggttggttac ggggcggtct tcctctctat
    16861 ttggctggtc gcgggaatgg ttcagtcgga tccgtctgtt gttggttcca tcgcttcttt
    16921 tgttaattac ctaagccaga ttatctttac cattgttatg gttggatttt tgggaaattc
    16981 tgtcagccgt gccatgattt ccatgcgtcg tattcgagaa attcttgacg cagagccagc
    17041 tatgaccttc aaggatatcc cagatgaaga gttggttgga agtcttagct ttgaaaatgt
    17101 gacctttacc tatccaatgg acaaggaacc gatgctgaaa gatgtgagct ttactattga
    17161 acctggtcaa atggttggtg tagttggagc gactggtgca ggaaagtcaa ccttggctca
    17221 attgattcca cgtctctttg atccacagga cggggccatt aaaatcggtg gcaaggatat
    17281 tcgagaagtg agtgaaggaa ccctgcgtaa aacagtttcc atcgttctcc aacgtgccat
    17341 tctttttagt ggaacgattg cagataactt gagacagggg aaggggaatg ctactctatt
    17401 tgaaatggag cgcgcagcca atattgccca ggctagtgaa ttcattcatc gtatggagaa
    17461 aacctttgaa agtccagttg aagaacgggg aaccaatttc tctggtggac aaaaacaaag
    17521 gatgtcgatt gcgcgtggga ttgtcagcaa tccacgtatt ctgatttttg atgattcgac
    17581 ctcagccttg gatgccaaat cagagcgctt ggtgcaagaa gctttgaata aggacttgaa
    17641 ggggacgaca accattatta ttgctcaaaa aattagctcg gttgtccatg cagacaagat
    17701 cttggttcta aatcaaggac gattgattgg tcaaggtacg catgcagact tggttgccaa
    17761 caatgccgtt taccgtgaaa tctatgaaac acagaaatga aagacaaact ataagaaaag
    17821 tcaatagttt tatctaaact atttcttatt tcaatttgat gatttggcga tgattttaga
    17881 gcacggcaaa aagcccttga aaaagtccat tttttcaaag gtaatcctgt gttaatttca
    17941 gaaattacat cactttttgt tcgtcaaatg gcagctcttt ttttaggata taaaacaggg
    18001 ttcggataag tttttttgca aggtggatga tggctacatt gtaatgtttt ccttgttcta
    18061 atttagtctt aagataggcc ttaaaagcag gcgaaaagcg agggcatgct ttggcagctt
    18121 gtatgagtac ctaccgcaga tgaggggaac tccgtttgac cattcttcct gctaaatcaa
    18181 tctgatctga ctgataaata gaagaatcca gtccagcgaa agcttgtaat tgagcaggat
    18241 tatcaaaggc atgaatattt cgaatctcag ctaaaatgac cgcccctaaa cgatccccaa
    18301 tcccagtaac cgtcgtgatg accgagttga actcagccat caagtcattg acacatgttt
    18361 ccgccttgtc aatgagcctc ttgtaatgtt tgatgttttc attacacgag ataaaacgtc
    18421 tatgcgttat caaactcatt accaattaaa acaaaaagct gtggttagat cctttcggaa
    18481 attgtcaagc gattggagga aatgaactaa tccacagcgg cttattccaa gtataccact
    18541 tgggctttgg cagtagctaa ctgcgctaaa tataatataa ggaggagtaa aatgaagaca
    18601 gttcaatttt tttggcatta ttttaaggtc tacaagttct catttgtagt tgtcatcctg
    18661 atgattgttc tggcgacttt tgcccaagcc ctctttccag tcttttctgg acaagcggtg
    18721 acgcagctag ccaatttagt tcaagcttat caaaatggca atccagaact tgtatggcaa
    18781 agcctatcag gaatcatggt caatcttggc ctgctggttt tggttctatt tatctctagt
    18841 gtaatataca tgtgtctcat gacgcgcgtg attgcagaat cgaccaacga gatgcgcaaa
    18901 ggcctctttg gtaagcttgc tcagttgacg gtttctttct ttgaccgtcg acaagatggc
    18961 gatatcctgt ctcattttac cagtgatttg gataatatcc tccaagcctt taacgaaagc
    19021 ttgattcagg tcatgagcaa tattgtttta tacattggtc tgattcttgt catgttttcg
    19081 agaaatgtga cgctggctct catcaccatt gccagcaccc cattggcttt ccttatgctg
    19141 attttcatcg tgaaaatggc acgcaaatac accaacctcc agcagaaaga ggtagggaag
    19201 ctcaacgcct atatggatga gagcatctca ggccaaaaag ccgtgattgt gcaaggaatt
    19261 caagaggata tgatggcagg atttcttgaa caaaatgagc gcgtgcgcaa ggcaaccttt
    19321 aaaggaagaa tgttctcagg aattcttttc cctgtcatga atgggatgag cctgattaat
    19381 acagccatcg tcatctttgc tggttcggct gtacttttga atgataagtc tattgaaaca
    19441 agtacagccc taggtttgat tgttatgttt gcacaatttt cacagcagta ctaccagcct
    19501 attatccaag ttgcagcgag ttggggaagc cttcagttgg cctttactgg agctgaacga
    19561 attcaggaaa tgtttgatgc agaggaggaa atccgacctg aaaaggctcc aaccttcact
    19621 aagttgcaag aaagtgttga aatcagtcat atcgtttttt catacttgcc tgataaacct
    19681 attttgaaag atgtcagcat ttctgcccct aaaggccaga tgacagcagt tgttgggccg
    19741 acaggttcag gaaaaacgac tattatgaac ctcatcaatc gcttttatga tgttgatgct
    19801 ggtggtattt attttgatgg taaagacatt cgtggctatg acttagatag tcttagaagc
    19861 aaggtgggaa ttgtattgca agattcggtc ttgtttagcg gaacgattag agacaatatc
    19921 cgatttggtg tgccagatgc tagtcaggaa atggttgagg tagcagcaaa agcaacccac
    19981 attcacgact atatcgaaag tttgcctgat aagtacgata ctcttattga tgatgaccag
    20041 agcatctttt caacagggca gaagcaattg atttcaatcg ctcgaaccct gatgacagat
    20101 ccagaagttc tcattctcga tgaagcaact tcaaacgtag atacggtgac agaaagcaag
    20161 attcagcatg ccatggaggt ggttgtagca ggtagaacta gtttcgtcat tgcccaccgc
    20221 ttgaaaacca ttctcaatgc agatcagatt attgtcctta aagatggaga agtcattgaa
    20281 cgtggtaacc accatgaact tttgaagcta ggtggctttt attcagaact ctatcacaat
    20341 caatttgttt tcgaataaga aagaagttgt cctatgtggg cagctttttc ttgtccataa
    20401 aaaatgttta tcacagcctt aaaaaaaaca tattagacga aagtcatttt gagtgatatg
    20461 ataggactat cgttagcatt cgaaaggaga ggcatcatgg ctagaacggt tgtaggagtt
    20521 gctgcaaatc tatgtcccgt agacgcagaa ggcaaaatca ttcattcatc tgtatcttgt
    20581 agattcgcag agatcattcg tcaagtcggt ggtctccctt tagtcattcc tgttggtgat
    20641 gagtcagttg tacgtgatta tgtggaaatg attgacaaac tcattttgac aggaggccaa
    20701 aatgttcatc ctcagtttta tggagagaaa aagaccgtcg agagcgatga ttacaatctg
    20761 gtccgtgacg aatttgaatt ggcactcttg aaggaagcgc ttcgtcagaa taaaccaatt
    20821 atggcaatct gtcgcggtgt ccaacttgtc aatgttgcct ttggtggaac cctcaatcaa
    20881 gaaatcgaag gtcactggca aggactacct ttcgggacat ctcactctat tgagacagta
    20941 gaaggaagcg tggtggctaa gctatttggg aaagaaagtc aggtcaactc agtccatcgt
    21001 caaagcatta aagatttggc acctaatttc cgtgtaactg ctattgattc aagagaccaa
    21061 actattgaag cgattgagtc tatcgacgag caccgcatta tcggtttaca gtggcatcca
    21121 gagtttctgg ttaatgaaga agatggcaat ttagaattat ttgagtattt attgaatgaa
    21181 ctgtaacgat tggaacatct ggtcgttctt tcttttattt tttcaaaatt tttagaatgt
    21241 ggcattttac gcaaacgttt gaattctgat aaaaattgga gaaattcgac aaaaaaactt
    21301 gaaaaaaacg aaggtaagcg ttatgataga aaagaagaaa tattggagga agaacatgtc
    21361 acatattaaa tttgattatt caaaagtttt agacaaattt gttgcaccac atgaagtgga
    21421 atacatgcaa tcacaagtaa cagcagcaga tgaattaatc cgtaaaggaa ctggtgctgg
    21481 tagcgacttc ttgggatggt tggaccttcc tgaaaaatac gaccgcgaag aatttgaccg
    21541 catcttgaaa gctgctgagc aaatcaagtc agacagcgat gttttggttg tgatcggtat
    21601 tggtggatct taccttggtg caaaagcagc aatcgacttc ttgaatcacc attttgctaa
    21661 cttgcaaaca aaagaagaac gcaaagctcc acaaatcctt tacgcaggaa actcaatctc
    21721 atctacttac cttgctgact tggtagagta tgttgcagac aaagacttct cagtaaacgt
    21781 aatttctaaa tcaggtacaa caactgaacc agcgattgct ttccgtgtct ttaaagaact
    21841 cttggttaag aaatacggtc aagaagaagc taacaaacgt atctatgcaa caactgaccg
    21901 ccaaaagggt gctgttaagg ttgaagcaga cgctaacggt tggggaacat ttgttgttcc
    21961 agatgatatc ggtggacgct tctcagtatt gacagccgtt ggtttgcttc caatcgcagc
    22021 atcaggagct gacataaaag ctcttatgga aggtgcgaat gcagctcgca aagactacac
    22081 ttcagacaaa atctctgaaa acgaagctta ccaatacgca gctgttcgta acatccttta
    22141 tcgtaaaggc tatgcaactg agatcttggt aaactatgag ccatcacttc aatacttctc
    22201 agaatggtgg aaacaattgg ctggtgaatc agaaggaaaa gaccaaaaag gtatctaccc
    22261 aacttcagcc aacttctcaa ctgacttgca ctcacttggt caatttatcc aagaaggaac
    22321 tcgtatcatg tttgaaacag ttgtccgtgt tgacaaacct cgtaaaaacg tgcttattcc
    22381 tactttggaa gaagaccttg acggacttgg ttaccttcaa ggaaaagacg ttgactttgt
    22441 aaacaaaaaa gcaactgacg gtgttcttct tgcccacaca gatggtgatg taccaaacat
    22501 gtatgtgact cttccagagc aagacgcttt cactcttggt tacactatct acttcttcga
    22561 attggcaatt gccctttcag gttacttgaa tgctatcaac ccatttgacc aaccaggtgt
    22621 tgaagcttat aaacgtaaca tgtttgccct tcttggaaaa ccaggatttg aagaattgag
    22681 caaagaactt aacgcacgtc tataatagaa gaaaagagtg gtttgcccac tctttttact
    22741 ctctttatcc atagaaattg gactcagcca agacttgtga tataatatag aaagcaaaaa
    22801 ggcagacgcc tagataatag gagaaactat gtcaaaagat atccgcgtac gttacgcacc
    22861 aagtccaaca ggactactac acatcggaaa tgctcgtaca gcattgttta attacttgta
    22921 tgcgcgccat catggtggaa catttctcat ccgtatcgaa gatactgacc gtaaacgcca
    22981 tgtcgaggat ggtgaacgtt cacaacttga aaaccttcgc tggttaggca tggattggga
    23041 tgaaagtcca gaatcacatg agaattatcg ccagtctgag cgtttggact tgtatcaaaa
    23101 atatattgac caactattag ctgaaggaaa agcctataaa tcttacgtta cagaagaaga
    23161 gttggcagct gaacgcgaac gccaagaagt agctggcgaa acaccacgct acatcaatga
    23221 ataccttggt atgagtgaag aagaaaaagc agcttacatt gcagaacgtg aagcagcagg
    23281 gatcatccca actgttcgtt tggctgtcaa tgagtcaggt atctacaagt ggcatgatat
    23341 ggtcaaaggc gatatcgaat ttgaaggtgg caatatcggt ggtgactggg ttatccaaaa
    23401 gaaagacggt tacccaactt acaactttgc cgttgttatc gatgaccacg atatgcaaat
    23461 ctctcatgtt atccgtggag atgaccatat tgctaataca ccaaaacagc ttatggtcta
    23521 tgaagctctt ggttgggaag ctccagagtt cggtcacatg accttgatta tcaactctga
    23581 aactggtaag aagttatcta aacgtgatac taatacactt caatttatcg aagattaccg
    23641 aaagaaaggt taccttccag aagcagtctt taactttatt gctcttcttg gttggaaccc
    23701 aggtggtgaa gatgagattt tctctcgtga agaattcatt aaactttttg atgaaaaccg
    23761 cctcagcaag tcaccagcag cctttgatca gaaaaaacta gactggatga gcaatgatta
    23821 tatcaagaat gcagaccttg aaaccatctt tgaaatggca aaaccattct tagaggaagc
    23881 aggccgtttg actgacaagg ctgaaaaatt agttgagctc tataaaccac aaatgaaatc
    23941 agtagatgag attatcccat tgacagatct tttcttctca gatttcccag aattgacaga
    24001 agcagagcgc gaagtcatga cgggtgaaac agttccaaca gttcttgaag cattcaaagc
    24061 aaaacttgaa gcgatgacag atgatgaatt tgtgacagaa aatatcttcc cacaaattaa
    24121 agcagtccaa aaagaaacag gtattaaagg gaaaaatctt ttcatgccta ttcgtatcgc
    24181 agtttcaggc gaaatgcatg ggccagaatt accagataca attttcttgc ttggacgtga
    24241 aaaatcaatt cagcatatcg aaaacatgct aaaagaaatc tctaaataaa gaggatacaa
    24301 taatggacat ctgggaaaag atgtacgaag aagcacagaa actatacaat ccacatgaag
    24361 tatcagactt tgtttatgcc aatcatgttg tagctgcagt agaagcagga gatggacaaa
    24421 tatttacagg attctgtatg gagggaacct gtggtgtttt ccatctctgc gcagaacggg
    24481 cggccctctt caatatgtac caattttcag gacaaactaa ggttaagaaa gtattagcct
    24541 ttcgagacaa accaccttat ggtggaagtt cagccatgcc ttgcggtgct tgtagagaat
    24601 tccttttaga gcttaatgct gaaaataaag atgcagaatt catgatggac tataatataa
    24661 gaaaaacagt taaagtcgca gaactaatcc cttattggtg gggaaaagaa cgtgcttcta
    24721 agtttaatga gcgatagaag agtcagtata attctggctt tttttactat aagttgaaaa
    24781 aataaaaaaa aatta
//
LOCUS       DAVHEX010000032        23593 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVHEX010000032 DAVHEX010000000
VERSION     DAVHEX010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23593
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.032"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            1..916
                     /locus_tag="VVL45_001231"
                     /pseudo
     CDS             1..916
                     /locus_tag="VVL45_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268095.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            1216..2055
                     /locus_tag="VVL45_001232"
     CDS             1216..2055
                     /locus_tag="VVL45_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEU4187407.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKTGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMFINVPLELVYITEKIINLGKITLGNNFDEIIYINLTDHIS
                     SSIERYKEGIIISNPLRWEISKYYKEEFELGKRALQIIKKELGIELPIDEAAFIALHF
                     VNANLENNFQESYKITEIIMGIEKIIQDFYCTEFNQDSIDYYRFITHIKLFAHRLVEN
                     TTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQTEYNYLLTSSELVYLMAHIRRLTK
                     NLD"
     gene            2073..3911
                     /locus_tag="VVL45_001233"
     CDS             2073..3911
                     /locus_tag="VVL45_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU4187408.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGAPVTPASYGSSVVPILIMV
                     WLMKYIEKIIAKLTLAITKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVEAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            3924..5339
                     /locus_tag="VVL45_001234"
     CDS             3924..5339
                     /locus_tag="VVL45_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEU4187409.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWVDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEN
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDELNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGSGTLNRY
                     KKMSFTWYKEVIESNGESLFK"
     gene            5821..6867
                     /gene="pheS"
                     /locus_tag="VVL45_001235"
     CDS             5821..6867
                     /gene="pheS"
                     /locus_tag="VVL45_001235"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEU4187410.1"
                     /translation="MSTIEEQLKALREETLASLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            6867..7376
                     /locus_tag="VVL45_001236"
     CDS             6867..7376
                     /locus_tag="VVL45_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4187411.1"
                     /translation="MIRKVEMADVEVLAKIAKQAFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            7453..9858
                     /locus_tag="VVL45_001237"
     CDS             7453..9858
                     /locus_tag="VVL45_001237"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEU4187412.1"
                     /translation="MLVSYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEVLSCEDVPETHLHVCQINVGEEEERQIVCGAPNVRAGIKVMVALPGARIADNY
                     KIKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILLEDAVPGEEVFSYLDLDD
                     EIIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETNEAAADALSVSIGTDK
                     APYYAARILDNVIIEPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDT
                     FEGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKS
                     SRVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASMIAELAGA
                     TVRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFT
                     VSVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTVTQKLRRQVRTIAE
                     GAGLTEIITYALTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNV
                     ARKNKNLALYEIGKVFEQTGNPREELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYA
                     KGILEALFTRLGLQVTYTATSEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIP
                     ETYVAELNLSAIEAALQPATPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVK
                     RLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNA
                     EVR"
     gene            complement(9926..10927)
                     /locus_tag="VVL45_001238"
     CDS             complement(9926..10927)
                     /locus_tag="VVL45_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU4187413.1"
                     /translation="MIKTFLSALSVILFSIPIITYSFFPSSNLNIWLSTQPILAQIYA
                     FPLATATMAAILSFLFFFLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NALPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            11083..11498
                     /locus_tag="VVL45_001239"
                     /pseudo
     CDS             11083..11498
                     /locus_tag="VVL45_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            11624..11890
                     /locus_tag="VVL45_001240"
     CDS             11624..11890
                     /locus_tag="VVL45_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU4187414.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGISQPVIARMETGKTSPQLDTVLKVLASLGKTLAIVPLEQGKS"
     gene            12151..14400
                     /gene="metE"
                     /locus_tag="VVL45_001241"
     CDS             12151..14400
                     /gene="metE"
                     /locus_tag="VVL45_001241"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEU4187415.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVAAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSTCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFEANRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            14464..15330
                     /gene="metF"
                     /locus_tag="VVL45_001242"
     CDS             14464..15330
                     /gene="metF"
                     /locus_tag="VVL45_001242"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEU4187416.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNDNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQTPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            16015..18228
                     /gene="pnp"
                     /locus_tag="VVL45_001243"
     CDS             16015..18228
                     /gene="pnp"
                     /locus_tag="VVL45_001243"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEU4187417.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQVVKDQVT
                     AVYEEKYADHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHQKDYKPKKEFTETSKDSE"
     gene            18244..18861
                     /gene="cysE"
                     /locus_tag="VVL45_001244"
     CDS             18244..18861
                     /gene="cysE"
                     /locus_tag="VVL45_001244"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEU4187418.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KHGFKLLARMYSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            18873..19757
                     /locus_tag="VVL45_001245"
     CDS             18873..19757
                     /locus_tag="VVL45_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4187419.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGELVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKAGQVIGTCTVDLSTNT
                     NYLYGLAILEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            19839..21182
                     /locus_tag="VVL45_001246"
     CDS             19839..21182
                     /locus_tag="VVL45_001246"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEU4187420.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFATADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            21175..21561
                     /locus_tag="VVL45_001247"
     CDS             21175..21561
                     /locus_tag="VVL45_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEU4187421.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQVLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            21565..22449
                     /locus_tag="VVL45_001248"
     CDS             21565..22449
                     /locus_tag="VVL45_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEU4187422.1"
                     /translation="MRAKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDTIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            22585..23040
                     /locus_tag="VVL45_001249"
     CDS             22585..23040
                     /locus_tag="VVL45_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187423.1"
                     /translation="MQILCYFTITVVAKPNNSGEVHLDVSIEDNQGGSGYNFSSVSSS
                     SQTAKYEGTVYNNNSSLYITIDKTSDATALLKLKLNNVDNQPATEVPSSGITVKLNAK
                     DNAGNWTSASNKKEVTVKIVSAKPTYPDKILVKNPDNIKDTEKMPLLKN"
     gene            23037..23183
                     /locus_tag="VVL45_001250"
     CDS             23037..23183
                     /locus_tag="VVL45_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187424.1"
                     /translation="MKEANKNHPAGAPTFAKGEGEHANDIVATYSDGTTYYVPLNDVT
                     KYAR"
     gene            23326..23496
                     /locus_tag="VVL45_001251"
                     /pseudo
     CDS             23326..23496
                     /locus_tag="VVL45_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         7317 a         4192 c         5190 g         6894 t
ORIGIN      
        1 ttaagattca cttcttttgg tcatttggaa attgaaaaga ccaaagcaag taattttata
       61 cagcttagtg attggattgc tactgactca gtgaggaatc agatgattct caaggatatt
      121 ctagcccaag tggcagaagg acggaatatc ttggttttag ttaatcgaat tcaacagata
      181 gatgtctttg aaaaattatt gaaagagaaa gaggttgatg actgttacat tattagcgga
      241 aaaaccaaag tccgagagag aacgagttta ctggagacgt tagaacagtt agataaaggg
      301 tttgttttgt tgtctactgg aaaatacatt ggcgaaggtt ttgacttacc tcagttggac
      361 acgcttatct tggcagcacc cttttcttgg aaaaataatt tgattcagta tgcaggtcgg
      421 attcatagaa actacaagga taagtctttg gtgcgtattt tcgattatgt ggatattcat
      481 gttccttatt tagaaaagat gtttcagaaa cgacaagtag cttatcgaaa gatggattat
      541 cgtgtcatcg agggtgagga gaaacaattc gtttatgttg atagaggact tagcagggga
      601 aagacaggaa tgtctgctta ttttacctta tgtgcaccag acaaaactga tgaattttct
      661 aaaagaattt aggattagtc aaattgagat atgtatacca gagacggttg caaataaagc
      721 atggctagac cagttgaaga gccagaaaat taaagtgtct tttactcaat caaaaatagt
      781 aacgcctatt cttttggtga ataagactat tgtttggtat ggtgcaatgc cattattagg
      841 gaaggtagat gagatgacca tattacgttt ggaatcagct agtatagttt ctgaactagt
      901 ggcaggttta cgatagagaa aatttttaaa aatttctatg tatgattttc atttctttag
      961 tgagactgtt gccattatca cattcgaatc acacaaaata aaaaaatttt tataagtact
     1021 tgacaaatag attgaaatat cataaaataa aaacggttac agagttatta attatttaag
     1081 cttcatgtca ccattaaaaa ttgaaataaa aggatgttat cactaataca agtgagcagg
     1141 aacctattta atcacatcag aagaagtttc ttgatgtttt taagtaggtt ccttttattt
     1201 taaaagggaa attttatgat cataaaacga atactaaacc acaatgccgt aattgcgcaa
     1261 agtaaaaaag atatcgatat tcttcttttt ggaaggggaa tagcttttgg aagaaaaact
     1321 ggagataaag taaatccaat tgatattgag aaaagttttt ttctcaaaaa tagagataat
     1381 atgacccgtt ttacagagat gtttattaac gttcctttgg agttggtgta catcaccgaa
     1441 aaaataatta acctaggtaa aataacattg ggtaataatt ttgatgaaat tatctatatt
     1501 aatttaacgg atcatatttc ttcgagcata gaacgttata aagaagggat tattatttcg
     1561 aatcccctac gctgggaaat atcgaaatat tataaagaag aatttgaact tgggaaaagg
     1621 gctttacaaa taataaaaaa agagttaggt attgaacttc caattgacga agctgcattc
     1681 atagcgctac attttgttaa tgctaattta gaaaataatt ttcaagagtc gtataaaatc
     1741 actgaaataa ttatgggaat tgagaaaatc attcaagatt tctattgtac tgagtttaac
     1801 caagattcta ttgattatta tagattcata actcatataa aattatttgc ccatcgcttg
     1861 gttgaaaata caacttattg tgacgatgat gatgaagact tgttagcatt aatgaaaaat
     1921 aaatatccta gagaatatga atgtggtgaa caggtagcaa tgtttataca aacggagtac
     1981 aattatttgc tgacttctag tgaattagtt tatttgatgg ctcatattcg tcgcttaaca
     2041 aaaaatttag attaatagaa taggagaata ccatgagtta taaagatacg gtacaaaaaa
     2101 tcctcgatgt aattggaggt gaaaaaaatg tcaatagagt tacccattgt gtaacacgtt
     2161 taagattaga attaaaagat gaaaatttag tcaatgatga tgatgtgaag aagataccag
     2221 gtgtaatagg tattatgaaa aagaatggac aatatcaaat tatacttggt aatgatgtag
     2281 ctaattatta taaagaattc gttaaacttg gcaattttga atccgattca gttgttcaag
     2341 ggcacaaagg gaatatttta gaaagaatca ttgagtatat cgctggttcc atgactccaa
     2401 tcattccagc aatgttaggg ggaggtatgt tgaaagtctt ggtaatcatt ttaccaatgc
     2461 ttggtatatt gcaatcagat tctcagacta ttgctttttt gacatttttt ggggatgctc
     2521 catattattt cttaccgctg ttattagctt attctgcatc acaaaaatta aaagtaacat
     2581 ctacattagc tatgtctgta gcaggtgtac ttctccatcc aaattttgtt caaatggtgc
     2641 aatcagggaa tcctcttagt ttatttggtg cacctgtgac accagctagt tatggttcat
     2701 cagtcgttcc aattcttatt atggtttggt tgatgaaata tattgaaaaa ataattgcta
     2761 aattaacact agctattact aagagttttt tgcaacctac gctagtatta ttagtatcaa
     2821 gctgtattgc cttagttgta gtcggaccta ttggagtaat tgttggtgaa ggattatcaa
     2881 atctagttgg gcaaatgtat ggtgtagctg gatggcttac attagctatt cttggtgcta
     2941 ttatgccatt tattgttatg actggaatgc attgggcttt tgcacctatt tttttggcgg
     3001 catctattgc tactccagac gtattaattc ttccagcaat gttagggtca aacttagctc
     3061 aaggggctgc ttcgatggct gttgcattaa agagtaaaaa taataataca aaacaaattg
     3121 cttttgcagc aggtttctca gccttacttg cagggattac cgaacctgca ttatatggtg
     3181 tgactttaaa atataaaaaa ccgctttatg cagctatgat tggtggtgga ttagcgggat
     3241 tatttgcagg tcttactagt gttaaagcat atctatttgc tgtcccatct ttgatagcgt
     3301 tgcctcaatt tatttattct gatgtgccat caaatattgt aaatgcttta attgtggcgg
     3361 tcatttcggt tgttattacc tttgtattag cttatatatt tggaatcgat gaagaagaga
     3421 gttctagcaa tttagaagtt gaagctggag tttcaaataa aaaaatgata ttttctccta
     3481 tatcaggaga aatcattccg ttaagcgatg tccaggataa aacattttca gataaactaa
     3541 ttggagacgg agtagcgatt atcccaagtg aaggtaaggt ttatgcacca tttgatggga
     3601 aaattacaaa tatttttccg actaagcacg caattggatt gaagagtgat gagggtgttg
     3661 agttactaat tcatattgga ttagatactg ttgagctaaa aggtcaaggt tttattagtc
     3721 atgtagaaga aggagacaga gttttcaaaa atcagttgat ttttgaaatg gacttgaatt
     3781 taatcaagac taaaggctac gaaacagtta caccagtaat tgtaacgaat accaatgatt
     3841 ttctagatgt attagtatta cctaataatc agacaatcga gcattctaag gaattactgg
     3901 taatattata atggaggaaa gttatgacca tttttccaga tgattttctt tggggtggag
     3961 ctgttgcagc taatcaagta gagggagcat ataatgaaga tggtaagggc ttatcagttc
     4021 aggatgtgtt acccaaaggt ggattaggag aagcaacaga aaatcctaca gaagataact
     4081 taaaattgat aggtattgat ttttatcata aatataagga agatatatcc ttgttttctg
     4141 aaatgggctt taatgttttt cgtacttcta ttgcatggag tagaattttt ccaaaaggag
     4201 atgaggaaga acctaacgaa gctgggttga aatattatga tgaattgttt gatgaactac
     4261 atgctcacgg gatagaacca cttgtaactc tttcacacta tgagactcca ttatatttag
     4321 caagaaaata tcatggatgg gttgatagga gaatgattca tttttatgaa aaatttgctc
     4381 gaacagtttt agaaaggtat aaagataaag ttaaatattg gcttacattt aacgaagtaa
     4441 actctgtttt ggaattacca tttactagtg gaggaataga tatacctaag gagaatcttt
     4501 cgaaacaaga attatatcaa gctatacatc atgaattagt cgcctcaagt ttggttacaa
     4561 aaattgctcg tgagattaat tcagagttta aggtgggatg tatggtatta gctatgccag
     4621 cttatccaat gactccaaat ccaaaagatg tatgggcgac tcatgagtat gagaatctaa
     4681 attatctatt ttcagatgtg catgttagag gttattatcc gaattatgca aaaagatatt
     4741 ttaaggaaaa tgacattaac atagagtttg cagctgaaga tgcagagtta ttaaaaaatt
     4801 ataccgtaga ttttttatcc tttagctatt acatgagtgt gactcaatct gctcttccaa
     4861 cacagtataa ttcaggagaa gggaatatta ttggtggttt agtaaatcct tatttagagt
     4921 cttcagagtg gggatggcaa attgatccaa ttggactacg tataatttta aatagatatt
     4981 atgaccgtta tcaaatccca ttatttatag tagaaaatgg attaggtgct aaagatcaac
     5041 taataaaaga tgaacttaat aacttaaccg tccaagatga ttatagaatt cagtatatga
     5101 aagaacattt attgcaagtt gctgaagctc tacaggatgg tgttgaaatt atgggctata
     5161 cgtcttgggg atgtattgac tgtgtttcta tgtccactgc acaactttct aaaaggtacg
     5221 gtctcattta tgttgatcga aacgatgatg gaagtggtac attaaatcga tataagaaaa
     5281 tgtcttttac ttggtataaa gaagtgattg aatcaaatgg agaatcctta ttcaaatagt
     5341 tttttattga tgactagcta ttatcacggt taaggtggca tttcattaaa tatggaataa
     5401 agaagtttgg aagacattaa attctaaact tctttatttt ataggggagt gtcaattaat
     5461 ttaattttgt tacaaaaatt ataaatagtt aatttagata aattgtttat ataaatttag
     5521 ataagaataa gctaacaagc agaagtaaga taggtcttgc aagaatggta aaaatctgat
     5581 aaactaagta gtaattgaat aggaatctga aagactagta gcccaaggga agtatatcag
     5641 ggaggagagc cgtgactgca agctctctat atgaaagctg ggtgaattca cttgcgcatg
     5701 gatttagaaa tgaaggtctg acttgtcaga tgaaaacgga tggtaccgcg tgtcaacgct
     5761 ccgagtggag tttttggcat gtggttttct ttttatctac gagagactga tggaggaaat
     5821 atgtcaacta ttgaagaaca attaaaagcg cttcgcgaag aaacgctggc tagcttgaag
     5881 cagattactg ctggaaatga aaaagagatg caagatttgc gtgtctctgt ccttggtaaa
     5941 aagggttcgc tcactgaaat cctcaaaggg atgaaagatg tttctgctga gatgcgtcca
     6001 atcatcggga aacacgtcaa tgaagctcgt gatgtcttga ctgctgcttt tgaagaaaca
     6061 gctaagctct tggaagaaaa gaaagtcgcg gctcaactgg ctagcgagag tatagatgtg
     6121 acgcttccag gtcgtccagt tgcgactggt caccgtcacg ttttgacaca aaccagtgaa
     6181 gaaatcgaag atatcttcat cggtatgggt tatcaagtcg tggatggttt tgaagtggag
     6241 caagactact ataactttga acgtatgaac cttccaaaag accacccagc tcgtgatatg
     6301 caggatactt tctatatcac tgaagaaatc ttgctccgta cccacacgtc tccagttcag
     6361 gcgcgtgcta tggatgccca tgatttttct aaaggtcctt tgaagatgat ctcgccaggg
     6421 cgtgtcttcc gtcgcgatac ggacgatgcg acccacagtc accaattcca ccaaatcgaa
     6481 ggcttggtag ttgggaaaaa tatctctatg gctgatcttc aaggaacgct tcagttgatt
     6541 gtccaaaaaa tgtttggtga agagcgtcaa attcgtttgc gtccatctta cttcccattc
     6601 acagagccat ctgttgaggt ggatgtttct tgcttcaagt gtggtggaga aggctgtaat
     6661 gtatgtaaga aaacaggttg gatcgaaatt atgggggccg gtatggttca cccacgtgtc
     6721 cttgaaatga gtggtatcga tgcgactgta tactctggct ttgcctttgg tcttggacaa
     6781 gagcgtgtag ctatgctccg ttatggaatc aacgatatcc gtggattcta ccaaggagat
     6841 gtccgcttct cagaacagtt taaataatga ttagaaaagt agaaatggca gatgttgagg
     6901 tgttggctaa aattgccaaa caagcctttc gtgaaacctt tgcgtatgat aatacggaag
     6961 agcagttaca ggaatacttt gaagaggctt atagtctgaa aactttgtca actgagttgg
     7021 gaaatcctga ctctgaaacc tatttcatta tgcatgagga ggagatagct ggttttctca
     7081 aagtcaactg gggaagtgct caaactgaga gagaattaga ggacgctttt gaaattcaac
     7141 gcctctatgt gctacaaaaa ttccaaggat ttggactagg taagcaactg tttgaattcg
     7201 cacttgaact tgctacaaaa aatagttttt cttgggcttg gctaggtgtt tgggagcata
     7261 atacaaaagc tcaagcgttt tataaccgat atggttttga aaaatttagc caacatcatt
     7321 ttatggttgg tcaaaaagta gatacggatt ggttactgag aaagaaatta aggtaagaag
     7381 agaattcttc tattgaaatg ataggaaagg aaaagaacca gtgtggcaat ggtcattctg
     7441 gagaactaaa ttatgcttgt atcttataaa tggttaaaag aattggtgga cattgatgtg
     7501 ccatcacaag agttggctga aaaaatgtca actacaggga tcgaggtaga gggtgtcgaa
     7561 tcaccagctg ctggtctctc aaaaattgtc gtcggtgagg tcttgtcttg cgaagatgtg
     7621 ccagagactc acctccatgt ttgtcagatt aacgttggcg aagaagaaga gcgtcagatc
     7681 gtttgtggtg ccccaaatgt gcgtgctggg atcaaggtca tggtggctct tccaggagct
     7741 cgtatcgctg ataactacaa aatcaaaaaa ggaaaaatcc gtggtttgga gtcacttgga
     7801 atgatctgtt cacttggtga attgggaatt tctgactcag ttgtgcctaa ggaattcgca
     7861 gatggcatcc aaatcttgct tgaagatgcc gtgccaggtg aggaagtctt ttcttaccta
     7921 gacttggatg atgaaatcat cgaactttcc atcacaccaa accgtgcaga tgccctttct
     7981 atgtgtggag tggctcacga agtggcagcc atctatgaca aggcagtcaa ctttaaagaa
     8041 tttactctaa cagaaactaa tgaagctgcg gcagatgccc tttctgtcag cattgggaca
     8101 gacaaggcgc cttactatgc agctcgtatc ttggacaatg tgattatcga accaagtcca
     8161 caatggttgc aaaaccttct catgaacgaa ggcatccgtc ccatcaataa cgttgtagac
     8221 gtgacaaact acatcctgct ctactttggt caacctatgc atgcttttga cttggacaca
     8281 tttgaaggga ctgacatccg tgtgcgtgaa gcgcgtgctg gtgaaaaatt ggtgaccttg
     8341 gacggtgaag aacgtgactt ggacgtgaat gacctagtca tcactgttgc agacaagcca
     8401 gtagcccttg caggtgtcat gggtggtcaa gcaacagaaa tctctgaaaa atctagtcgt
     8461 gttgtccttg aagctgctgt tttcaatggt aaatctatcc gtaagacaag tggtcgcctg
     8521 aaccttcgtt cagaatcgtc ttctcgcttt gaaaaaggca tcaatgtggc aacagtcaat
     8581 gaagcccttg atgcggcagc tagcatgatt gcagaacttg caggtgcgac ggtgcgtaag
     8641 ggtatcgttt cagcgggtga gcttgatacc tctgatgtgg aagtttcttc aacccttgct
     8701 gatgttaacc gtgtcctcgg aactgagctg tcttatgctg atgtagaaga cgtcttccgt
     8761 cgtcttggtt ttggtctttc tggaaatgca gacagcttta cagtcagcgt accacgtcgt
     8821 cgttgggata tcacaatcga agctgacctc tttgaagaaa tcgctcgtat ctatggatat
     8881 gaccgcttgc caactagtct accaaaagac gatggtacag ctggtgaatt gactgtgaca
     8941 caaaaactcc gccgtcaagt tcgtaccatt gctgaaggag caggtttgac agaaatcatc
     9001 acctatgctc taacaactcc tgaaaaagca gttgagttta cggctcaacc aagtaacctt
     9061 acggaactca tgtggccaat gactgtggat cgttcagtcc tccgtcaaaa tatgatttca
     9121 ggtatccttg ataccgttgc ctacaacgtg gctcgtaaga ataaaaactt ggccctttac
     9181 gagattggaa aagtctttga acaaacaggt aatccaagag aagaacttcc aaatgaaatt
     9241 aacagttttg cctttgcctt gacaggcttg gttgctgaaa aagatttcca aacagcagca
     9301 gttccagttg atttcttcta tgctaaggga atccttgaag ccctatttac tcgcttggga
     9361 ctccaagtaa cctatacagc aacatctgaa atcgctagcc ttcatccagg tcgtacagca
     9421 gtcatttcac tcggtgacca agttcttggt ttccttggcc aagtgcatcc agtcactgct
     9481 aaggcttatg atattccaga aacgtatgta gctgagctta acctttcagc tatcgaagct
     9541 gcgcttcagc cagcgactcc atttgtagaa atcaccaaat tcccggcagt cagccgtgac
     9601 gttgcccttc tcctcaaggc agaagtgact catcaagaag ttgtagatgc tatccaagct
     9661 gccggcgtga aacgtttgac agatatcaaa ctctttgacg tcttctcagg tgaaaaactg
     9721 ggacttggta tgaagtcaat ggcttatagc ttgaccttcc aaaatccaga agacagctta
     9781 acggacgaag aagtcgcacg ctatatggaa aaaatccaag cgtctctcga agaaaaagtc
     9841 aatgcagaag tgcgttaaac gatagtagga tacaaattcc ccctctagtt tatgaacatt
     9901 agaggggtga ttttatataa aataattaaa atgtgatttc tgtaaaaata catctatgat
     9961 cagagttttg aaaacttaca atgtctaaat ctttaacata gtagtggttt ttaggcaata
    10021 aaatatgatc tattgttgca ttaaaaagtt ttggactttg gctattccaa gttcctcttt
    10081 caaaaggtgg cagtgcattt aatgcgtccc tatgagagct tatttttgca agtgctccat
    10141 gacgcatagt tgcattaaaa tcacctgcaa taatagcctt tggatatttt gaagccaatt
    10201 gattatgaat gatgtttaag tcttgcttcc agatttccat taaacctggc agaggaggag
    10261 cagtatgcaa ggcaatgata tctggtatat tttgttttct cgaatgtaat acaattgtcc
    10321 cgaaccgtgt tgtatgaaaa gttttagctt ctgtatagaa accataactt ttcttgacaa
    10381 tcacagtcac aggagctatt ccactattgg taggtggaga agtgaaaata tcatagttgg
    10441 ccatagaaag tccaacttga tgaaacaata gtttgattct ctggttttct tgctcacctc
    10501 tgatattggt agctagttca gggaatatag ccatatcggc gtcaaaatgg ctaaaaattc
    10561 gctcaatatg ttgtgcttct atttgattag cgacgttcca agttactaat tttaaggttt
    10621 tagtcttatt tgatgcagaa gaaagggttt tatctgttcc gaataatagt aatatgagcg
    10681 atagtaagag caaaatgcca gagtaaaacc gtatttgttt atttttcttg taaaaagata
    10741 ggaaaaaaaa taagaaactt aaaatagcag ccatagttgc agtagctaag gggaaggcat
    10801 aaatctgtgc caagataggt tgggtagata gccaaatgtt aagattagaa gatgggaaaa
    10861 aagaataagt tatgataggg atagaaaaga gaatgaccga aagggcagag agaaatgttt
    10921 taatcattgt gtgagtacct caatttgcaa tggttgctcg gagctaatgt gactttgtga
    10981 ctttacttga ttgatattct ttttcttgtc ttcctcatag attaagtaca acccaagtaa
    11041 aaaaagtcca atttaagatt gaaatgatta aaaggcacag aaatgcaaga caattatact
    11101 acaaaaggca aacatttgac aatcgatagc cgtcgcttaa tcgaacgatg gaaaaaagaa
    11161 ggaaaatcaa atagagaaat tgcctctcta cttggaaaag ctcctcaaac tatccacact
    11221 gaaatcaagc gtgggacagt ccgacaatgt cttggaaaag ggcgcttcaa agagatttat
    11281 tctgccgact acgctcaaca gtcttaagaa actaaaggaa aagattctcc actatacata
    11341 accaaaaaca taaccaaaaa ttttcgcctg aaatgatggt gatggctaaa ggggttaacg
    11401 tgggaatttc aaccatttac tattggattc atcgtggaaa attggggtta agcaagcagg
    11461 aactgcttta tcctagaaaa ggaaaatcgc ttaagaaata ggctagcacc aactttaaac
    11521 ccactggtca atctatcgaa tcataattcg ctcatttttt taaaaataca tagacgtaaa
    11581 aaaaagaggg ttaagtgatg aagaatagtg ctattgggag taattggaag gatgtccatc
    11641 tgagctcttt agcaaggagg aaatccttga aagtgatatg cgagtggcta tcatgagcga
    11701 gttgattgag gctaggtatg aacaaggaat cagtcagaaa aagctggaag aagtcagtgg
    11761 aataagccag cctgtcatag ctaggatgga gacaggaaag actagtcctc agttggatac
    11821 agtcttaaaa gtcctagcca gtttaggaaa gacactagca atcgtcccac ttgaacaggg
    11881 aaaaagttga ctagaatcag ttatagtctt ttctaataac aggtcatagt cacttataag
    11941 aattactaat aacatgtttt agtattctaa ttggaatata gctgtaaagt aagaattaca
    12001 aaggatttga gacgtttgct tcaagtcttt ttcttttgtc cgaaacagat atatagtttc
    12061 aaactatatc aaaccctaat tttttacaat tatacagacg ctttcttttc tgttaagata
    12121 gtttcaacaa caaattttgg aggacacatc atgtcaacta caatcatcgg tttccctcgt
    12181 ttgggcgaat tccgtgaatt aaaatttaca actgaaaaat actttagaaa agaaatctca
    12241 gaagaagaac ttttagcagc agcaaaagac ttgcgtgcta aacactggaa cattgtcaaa
    12301 gaaaaaggca tcactgaaat tccatcaaat gacttttctc actatgataa cttcctagat
    12361 gcggctttcc ttttcaacgt ggtgcctgca tccgttcaaa acttggactt gtctgacctt
    12421 gagcgctact tcgctttggg acgtggttac cagggagaaa aaggggacgt tcgcgccctt
    12481 ccaatgaaga aatggttcaa cactaactac cactacatcg ttcctaaatt tgaaaaagac
    12541 actcaagtaa aattggctgg tcacaagatt ttcgatgagt tccaagaagc caaagaactt
    12601 ggattgaaca ctcgtcctgt tcttgtaggt cccttcactt tccttcaatt gtcagacttt
    12661 gaagaaggcg tgaaagcaga cgacttcgta gatagcttag tggctgctta ccaagaagtt
    12721 tttgctaaat tggctgaact tggtgcgact cgcatccaat tggatgaagc ggctcttgta
    12781 aaagacttga cagctgaaga aaaagctctc ttcttgaacc tttacaacaa actcttggct
    12841 gacaaaaaag gtcttgaagt cttgcttcaa acttatttcg gtgatgttcg tgacgtatac
    12901 gctgaccttg taaacttacc agtagatgct atcggtcttg actttgttga aggtaagaaa
    12961 actcttgaac tcgttaaagg tggcttccca gctgacaaga ctctctatgt aggtattgtc
    13021 aatggtaaaa acatctggcg caacaactac gaaaagagct tggctgttct tgagcaaatt
    13081 ccagctgaaa acatcgtttt gacaagcact tgctcacttc ttcatgtgcc atttacaact
    13141 gctaatgaag aatttgaacc agcactcttg aaccactttg cctttgcagt tgaaaaattg
    13201 gatgaaatcc gtgacttgga tgctatccga aatggtcaag gttcagaagc tcttgcagcc
    13261 aacaaagaac tctttgcgac tgagcgtgtt ggtgaaaatg ctgaacttcg tgcgcgtatc
    13321 gctggcttga cagacgcaga ctacactcgt ttgccagcct ttgcagaacg tgaagctatc
    13381 caagaagaag ctttcaaact tccagctctt ccaacaacaa cgattggttc attccctcaa
    13441 acaaaagaag ttcgtgccaa acgtttggct taccgtaaag gtgaattgtc tcaaaaagag
    13501 tacgacgctt tccttgctga aacgattgac gaatggatca agtggcaaga agatattgac
    13561 tttgatgtcc ttgttcacgg tgaatttgag cgtaatgaca tggttgagta cttcggtcaa
    13621 aacttgtcag gatacctctt ctctaaaaat ggatgggtac aatcatacgg tatgcgtggg
    13681 gtaaaaccac caatcatctg gggtgatgtc actcgtctta accctatcac tgttaaatgg
    13741 tctagctatg cacaaagtcg tacaaacaaa cctgttaaag gtatgttgac tggacctgtt
    13801 accatcctta actggtcatt cccacgtgaa gacatctcta tcaaggattc aactctccaa
    13861 atcgcccttg ctatcaagga tgaagtactt gaccttgaag ctgctggtgt gaaaatcatc
    13921 caaatcgacg aggctgctct tcgtgaaaaa ttgccactcc gccgtagcga ctggtacgaa
    13981 gactaccttg actgggctat tcctgccttc cgcttggtac actcaacagt agcgccagac
    14041 acacaaatcc acactcacat gtgttactca gaatttacag atatcatccc agctatcgat
    14101 aacatggatg cagacgttat ttcctttgaa gctaaccgtt caaaccttga aatcttggac
    14161 gaactcaaag cgaaaaactt ccaaacagaa gtgggacctg gggtttacga tatccactca
    14221 cctcgtgtac caaatgaagg cgaaatcgac aacacaatcg aagccatcct tgctaaagtg
    14281 ccaagcaaga aagtttggat caaccctgac tgtggtttga aaacacgtgg tatcccagaa
    14341 acaaaagaaa gcttgatccg ccttgttgaa gcagctaaag ctgcgcgtga gaaattgtaa
    14401 gattggattt ctctccatac agtggttgtt caaaaagaaa tcaactagaa aaggttatta
    14461 catatgtcac gccaaacacc gtcactctca tttgaagtgt tccctccaaa cccagccgtg
    14521 ggtaatgata acattatttc agccttgcag gatatgcagg agttggctcc ccattttatc
    14581 agtgtaactg ccagcaataa taaatttaat atcaaggaaa cgacggttcg tttggctgac
    14641 tttatccaaa atgatttggc gattccgacc attgcccact tgccagctat ttatctaact
    14701 aaggacaagg ttgctgaaac tattgctgac ttggacaaag ttggggtgca gaaaatcttg
    14761 gctcttcgtg gggatattat tccagacgtg gaaccacaaa aggatttccg ctatgcaacc
    14821 gacttgattg agtttatcaa ggaacaaacc cctcactttg atattattgg agcttgctat
    14881 ccagaagggc atccagattc gccaaatcag atttcagata ttcaaaatct taagaagaaa
    14941 gtggatgcag gctgttcgag cctcgtaact cagctcttct ttgacaatga gcgcttctat
    15001 gattttcagg acaagtgtat cttggctggg attgatgttc ccattcatgc aggaattatg
    15061 ccaattctga atcgaaatca ggctctccga ctcttgaaga cttgtgagaa tatccatctt
    15121 ccacgcaaat ttaaagccat cttagacaag tatgagcatg accctgagtc gctcagagca
    15181 gcaggacttg cctatgcagt ggaccaaatc gtggacttgg taactcagga tgttgccggt
    15241 gtgcatctct atacgatgaa taatgctgat acagcaaaat acatccatca agcaacccat
    15301 gccttgttta atcaccagtc tctaggataa taaaagcaaa ccattcttct caggtgaggg
    15361 gaatggttcc tttttaatgg taaagacctc actttttaag aaaaatatga taaaataaat
    15421 tctgtacgta cttgatacaa agatgaaggt attaaataat acaatgcatt taaactgtaa
    15481 gatttaacga cacgagtgag tcaaatcgat gttatttgac gagagagtta gtaaagcatt
    15541 tagctaatcg cccgatagcg attagcgtga aagctttaga tactttagta ttaaaccttt
    15601 ctaatgatgt atcttgaaaa ctgaacttgc ctacaactct gtggaaaaag ataaatctcc
    15661 ctaggagtag tcgctccgtc gttcgatttc ctatcttcct ttgagttgct tgacggcttg
    15721 gtatcttgat gacttgaatt ttggtctcat ttcttaggaa aaaagataag cttcctagca
    15781 ctcgtcgagt gcgacgtcac cttcctattt ttctacagaa atgttcggca agccgaaccg
    15841 tccaaaatat cttgtgtaat tgaacacggc cgaaaagctg tgtaaaaaag ataaactgtc
    15901 ttgtcttcat cgaagacttc gtcagtttcc tatttttact ttgcttttga cgtccttggt
    15961 atcttgatct ttgtaggcaa ggcgtataat ttcatcaatc caaaggggat taaaatggca
    16021 aaacaagtgt ttcaaacgac ttttgcgggt cgtgagttaa ttgtagagac tggtcaggtt
    16081 gctaagcaag caaatggatc tgttgtcgta cgttacggtg agtcaactgt cttgactgct
    16141 gccgttatgt ctaagaaaat ggcaactggg gatttcttcc cactccaagt caactacgaa
    16201 gaaaaaatgt atgcggctgg gaagtttcct ggtggcttta tgaaacgtga aggacgtcct
    16261 tcaacagatg cgaccttgac agcgcgtttg attgaccgtc cgattcgtcc tatgtttgcg
    16321 gaaggtttcc gtaatgaagt ccaagtcatc aatacagtgc tttcttatga tgaaaatgca
    16381 tctgcaccaa tggctgctat gtttggttca tctttggcac tgtctatttc agatattcca
    16441 tttgacggac caattgctgg ggtacaagtg ggatatgtag atggccaaat catcatcaac
    16501 ccaagtcaag aacaagcaga gcaatctctt cttgaattga cagtagctgg aaccaagcac
    16561 gctatcaaca tggtagagtc tggtgccaaa gaattgtcag aagaaatcat gttggaagcg
    16621 ctccttaaag ggcacgaagc tgtcaaagaa ttgattgcct tccaagaaga aatcgttgct
    16681 gctgtcggta aagaaaaagc agaagtggaa ttgcttcacg tggatgctga attgcaagct
    16741 gaaatcattg cagcctacaa cagtgacctc caaaaggcag ttcaagtaga agagaaattg
    16801 gcccgtgaag ctgcgactca agtagtgaaa gaccaagtga ctgccgttta cgaagaaaaa
    16861 tatgcggacc acgaagaatt tgaccgtatt atgcgtgatg tggctgaaat cttggaacaa
    16921 atggaacacg cagaagtgcg acgtttaatt acagaagaca aggtgcgtcc tgatggtcgt
    16981 aaggtcgatg aaatccgtcc tttggatgcg gttgttgact tccttcctcg tgtacatggt
    17041 tcaggtctct ttactcgtgg gcaaactcaa gctctttcag tcttgacctt ggctccgatg
    17101 ggagaaactc aaatcattga tggtttggat ccagagtaca agaaacgctt tatgcaccac
    17161 tataacttcc ctcaatattc tgtaggggaa acaggtcgtt acggtgcgcc aggtcgtcgt
    17221 gaaatcggtc acggtgccct tggtgagcgt gctcttgctc aagtcttgcc aagcttggaa
    17281 gaattcccct acgctatccg tctagtagca gaagttttgg aatcaaacgg ttcttcatct
    17341 caagcttcta tctgtgcggg aactcttgcc cttatggctg gtggtgtgcc aatcaaggcg
    17401 ccagtagctg gtattgctat ggggcttatc tcagatggaa ataactacac agtattgaca
    17461 gatatccaag gtttggaaga tcactttgga gatatggact tcaaggttgc aggtactcgt
    17521 gatgggatta cagcccttca aatggatatc aagattcaag ggattactgc agaaatcttg
    17581 acggaggctc ttgctcaagc caagaaagcg cgttttgaaa tccttgatgt cattgaagca
    17641 accattccag aagttcgtcc agaattggct ccaactgctc cgaaaattga tacgatcaag
    17701 attgatgtgg acaagattaa gattgtcatc ggtaagggtg gagaaaccat cgacaagatt
    17761 atcgctgaaa caggtgttaa gattgatata gacgaagaag gaaatgtgtc tatctactct
    17821 agtgaccaag atgctattaa ccgtgccaaa gaaattattg ctggtttggt tcgtgaagcc
    17881 aaagtggatg aagtttaccg tgctaaagtc gttcgtatcg agaaatttgg tgcctttgtt
    17941 aacctctttg ataagacaga tgcccttgtt catatctctg agatggcttg gactcgtacc
    18001 aatcgtgtag aggatttggt agaaatcggg gatgaagttg atgttaaggt tatcaaaatt
    18061 gatgaaaaag gccgtatcga tgcctctatg aaggctcttc tacctcgtcc gccaaaacct
    18121 gagcatgatg aaaaaggtga aaagtctgag cgccctcacc gcccacgtca tcaaaaggat
    18181 tacaaaccta agaaagaatt tacagaaaca tcaaaagatt cagaataaga aaaggagaaa
    18241 tgtatggggt ggtggcgcga aaccattgat attgtaaaag aaaatgatcc agcggcccgc
    18301 accactttgg aggttttgct gacttatcca ggtgtcaagg ccttggcggc ccaccgtctc
    18361 tcgcattttc tctggaagca cggcttcaaa ctattagctc gtatgtacag tcagttttgg
    18421 cgcttttgga ctcagattga gattcatcca ggagcccaga ttgattcagg tgtttttatt
    18481 gaccatggtt ctggtctggt gattggagag acagcgattg ttgaaaaagg cgttcttctc
    18541 tatcacggag tgactctcgg gggaacaggg aaagactgtg gcaaacgcca tccgactgta
    18601 cgaaagggag ccctcatatc agcccatgcc caagttatcg ggcctgtgga aattggtgaa
    18661 aatgccaagg tcggtgctgc agcagttgtc gtagcagatg tacctagtga cgtgacggtt
    18721 gtcggtattc cggccaagat tgtccgtctt catggtaaga aagatgagcc tgtcattcac
    18781 gaagtcgaag aaaaacgaga gtattatgtc aataaactcg agcaggctaa agatgccagt
    18841 cacagatcgt ctggtttgta gaggatacct atatgattca agcaagaaac aagttaagcc
    18901 aagaggagct atctgaggcg aaaaaagtaa ttaactgttg ccaaaactat gacggtacct
    18961 atcgcgatcc ctatctctct aacatgctta attttgaccc aaacatgccc gcctttttcc
    19021 tttattatga aaaaggcgaa cttgttggtt tattaactgt ctatgcagat gaccaagatg
    19081 tagaagtgac gatactggtt catccaggtc atcgccgtca ggggattgcg cgtgcattgt
    19141 ttactagttt tgagagagaa acagcttctt tccccattcg gtcagtcact tttcagacag
    19201 aacgtatttt tttagagaat catcctgact ttgccagtaa ctggggacta atcgaggatg
    19261 aagagacaga aacttggtta ggtaaggata gaagacctta tccgttagca aatgtttccc
    19321 atcttgaagt tttgttagca gatagttcgt atcaggatca aattagtcaa ttaaaatttc
    19381 aggcattttc agaggaacat gaatcgagag aagttgtgga tagatatgtc gctgaagctc
    19441 tgaaagatcc agaaagccga ctatatattt tattaaaagc tggtcaggtt attggaactt
    19501 gtacggttga tttatcgact aatacgaatt acctctacgg tttagcaata ttggaacctg
    19561 aacgtggaaa aggctatgga agctacttag caaaatccct cgtcaaccaa ctaattgagc
    19621 aaaatgacaa ggaatttcag attgcagtgg aagatagcaa tgtaggtgcc aaacgtttgt
    19681 atgaaaaaat tggctttgtc aaacagactc aggtggttta tctgaatgag aaaggagcaa
    19741 gggattccga agtgtagaga tattcggact gaaatttact tgaactttta gtgatgaaac
    19801 taattgttct tggatttcag ctttcctgat tatgatttat gattaaaatc tatgacacca
    19861 tgtctcgtga tttgcgagaa tttgtcccga ttgaggacgg caagatcaag atgtatgttt
    19921 gtgggccaac ggtgtacaac tatatccacg taggaaacgc ccgttcgacg gtagcttttg
    19981 atacgattcg tcgctatttt gagtaccgtg ggtataaggt tgcctatatt tccaatttta
    20041 cagatgtgga tgataagatt atcaaccgtg ccagggaaga aggtatcacg cctcaggagg
    20101 ttgcggataa gtacatcgct gcctttcgtg aggatgtgac ggcattgggc gtgaaacctg
    20161 cgactcgcca tccgcgtgta gtggagttta tggcagacat catccgtttt gtggaagact
    20221 tgatcgagaa aggctttgcc tatgagagtc aaggggatgt ctatttccgt gtagaaaaat
    20281 cccacaacta tgctaaattg gctaataaaa ccttggaaga tttggagcta ggtgcttcag
    20341 gtcgtaccga tgaagaaacg gctcgtaagg aaaatcctgt agactttgct ctatggaaat
    20401 cttccaaacc aggtgagatt tcttgggata gtccttgggg acctggacgt ccgggttggc
    20461 atattgagtg ttcagtcatg tcgacagaga ttttaggtga taccattgat atccacggtg
    20521 gtggagctga cctagagttt ccacaccaca ccaatgaaat tgcccagtct gaagctaaaa
    20581 caggcaaggc ttttgctaac tactggatgc acaatggctt tgtcaatatc gacaatgtca
    20641 aaatgtctaa gtccttgggt aacttcatta cggtacacga tgcccttaaa actcttgatg
    20701 ggcaagtact tcgtttcttc tttgcgaccc agcactaccg taagcctatc aacttcacgg
    20761 aaaaggcagt tcgcgatgcc gagaccaatc tcaagtatct caagaatact tacgaacaac
    20821 catttacagg aaatgtggat gcccaagagt tacaaaactt taaagataag tttgtagcag
    20881 ctatggatga agattttaat gctgccaacg gtatcacagt tgtctttgaa atggccaaat
    20941 ggatcaactc agggaactat gatgcaagtg tcaagcaagc tcttgcagat atgttagaaa
    21001 tttttggaat tgtctttgtt gaggaagttt tggatgcaga gattgaagac ttgattcaaa
    21061 aacgccaaga ggcgcgtgcc aatcgtgact ttgcgacagc agaccaaatc cgtgaccaat
    21121 tggttactca aggaattaag ctccttgata ccaaggatgg agtgaggtgg acacgtgatt
    21181 gatgtcaatc tcattaacgg gattgcgcta gcctttgagg gggatgcggt gtattctatg
    21241 tatattcgcc gtcacctcat cctcaaaggt atgaccaaac ccaataaact ccatcaagaa
    21301 gcaactaagt acgtgtcagc caaggctcag gctcgcctga ttgctctcat gttggaggag
    21361 caggtcctaa cggaaaaaga agaagaaatc tacaaacgtg gccgcaatac caatagccac
    21421 acaaaggcta aaaatgcaga tgtcgtgact tatcgtatgt ccacgggatt tgaagcggtt
    21481 atgggctatc tccatatgac tgagaatctg gaacgtcttg agagtttggt ttcatggtgc
    21541 atccaaaaag tggagggcta gaacatgagg gcaaaagaac tacaagactg gtttcctgag
    21601 gctcggattt cagaccaacc agtagagaaa gagggctatc tcacgctccc tttagcttct
    21661 cagcagtgga ttttgctgga ggaagctggg ctcagcgagc gtgaaaagca gttggttgcc
    21721 cttttgaccc agcaggagca ggctcgttcg ctaaaccctt ggtattccta tctggttgag
    21781 ggcaagggac aggcaccgca agtttttaaa aagattcagt tggtttattg ccatctttct
    21841 tattttcagc aggaaaatct ggcttcttgg ctagatatga tgcggactct ttttccgaat
    21901 tgtcagacag tgctacaggt cggagctcag gattatgttt tcgtgcttca acaagacaaa
    21961 tacacttctg taagagatat tttaagtgat acgattgaag cggttgagta tgactttgga
    22021 cttcgtcttt ctatcatgtt gggtcaggtt tggtctcaga cgggacatca agccctatca
    22081 gacttaatca aagctgagcg ggatttgttc aagacatggt ggcgtcaggg tcaccaaggt
    22141 gttcatactt tttctcagct ctatctttgg agtatgggag aaagactcgt ggacttgaag
    22201 ccaatcaagg aatgtctaca ccagatgatt ttggatcaag atcagattca ggaaatcatt
    22261 ctctctcttt gggaaaatag tgctgttctc actaaaacag cccagcaact ctatctgcac
    22321 cgcaattctc tccaatacaa gattgataaa tgggaagagt tgacagggct tcagttgaaa
    22381 gagttgaccg acctgacctt gtgttatcaa ttgattttag gttctttgtc aactatagtt
    22441 ggtttgtaaa gaagttaata tttggagaag aggattgcca tcttctccat tttatgtgca
    22501 gagttatagt ggcttgatgc tgggaaagta cactgtgact gctaaaacat tcctagaagc
    22561 tgctttgatt tccctaatct atttatgcaa attttatgtt attttactat aacagttgtt
    22621 gctaagccaa ataatagtgg ggaagttcat ttagacgtaa gcattgaaga taatcaggga
    22681 ggtagtgggt ataatttcag ttctgtttca agtagctcac aaacagctaa atatgaagga
    22741 actgtttata ataacaattc atcattatat ataacgattg ataaaacgtc tgatgcaaca
    22801 gctcttttga aattaaagtt gaataatgtt gataatcaac ctgctactga agttcctagt
    22861 tcaggaatta ctgtaaaatt aaatgctaaa gataatgctg gaaactggac aagtgcttcg
    22921 aataaaaaag aagtaacagt aaaaattgtt tctgctaaac cgacatatcc agacaaaatc
    22981 ttagtgaaaa atcctgataa tataaaagat acagaaaaaa tgccattatt gaaaaattga
    23041 aagaggcaaa taaaaatcat ccagcaggag ctccaacctt tgctaaaggt gaaggagagc
    23101 atgcaaatga tattgtagca acttattcag atggtacaac ttattatgta ccgttaaatg
    23161 atgtgacaaa atatgcgagg tagtggctgt accactcact tattcacctc cccgtgattt
    23221 gtagtagtga taggttttct cactattatt ataaaacaaa ataaagatca caacactttt
    23281 tcattctgtg ttgtgccttg agtgaaacga aaggaatgaa ttataaatat gaaaagtata
    23341 gtcactagca tagatgagcg cttgcgtact cgcctacgag tgattatctg gaagcaatgg
    23401 aagaagaaat cgagacgatt atggggattg cttaagttag gggttcctaa atggatagca
    23461 gataaggtat ctggctgggg cgaccactat caattatagt aaaatgaaat aagaatagga
    23521 cgaattgttc aggacagtca aatcgatttc taacaatatt ttagaagtag aggtgtacta
    23581 ttctagtttc aat
//
LOCUS       DAVHEX010000033        23040 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVHEX010000033 DAVHEX010000000
VERSION     DAVHEX010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23040
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.033"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            1..651
                     /locus_tag="VVL45_001252"
                     /pseudo
     CDS             1..651
                     /locus_tag="VVL45_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841243.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            complement(952..2841)
                     /locus_tag="VVL45_001253"
     CDS             complement(952..2841)
                     /locus_tag="VVL45_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEU4187425.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWSRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVIDRG
                     NVSIISEGDAVGLRLVKEDGLSSFEKDCLNLAFSGKKEETLSNLFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGGEKALQVGMG
                     ALTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDQAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSTFYHSTAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            2979..4544
                     /locus_tag="VVL45_001254"
     CDS             2979..4544
                     /locus_tag="VVL45_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEU4187426.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPILLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            4544..5284
                     /locus_tag="VVL45_001255"
     CDS             4544..5284
                     /locus_tag="VVL45_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU4187427.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEKIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(5393..5608)
                     /locus_tag="VVL45_001256"
     CDS             complement(5393..5608)
                     /locus_tag="VVL45_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187428.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNYEKFED"
     gene            complement(6343..7593)
                     /gene="ilvA"
                     /locus_tag="VVL45_001257"
     CDS             complement(6343..7593)
                     /gene="ilvA"
                     /locus_tag="VVL45_001257"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEU4187429.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIKTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
     gene            complement(7643..7999)
                     /locus_tag="VVL45_001258"
     CDS             complement(7643..7999)
                     /locus_tag="VVL45_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU4187430.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEVFGDE"
     gene            complement(8237..8473)
                     /locus_tag="VVL45_001259"
     CDS             complement(8237..8473)
                     /locus_tag="VVL45_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187431.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMNFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKH"
     gene            complement(8580..9602)
                     /gene="ilvC"
                     /locus_tag="VVL45_001260"
     CDS             complement(8580..9602)
                     /gene="ilvC"
                     /locus_tag="VVL45_001260"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU4187432.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            complement(9668..10144)
                     /gene="ilvN"
                     /locus_tag="VVL45_001261"
     CDS             complement(9668..10144)
                     /gene="ilvN"
                     /locus_tag="VVL45_001261"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEU4187433.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            complement(10137..11837)
                     /locus_tag="VVL45_001262"
     CDS             complement(10137..11837)
                     /locus_tag="VVL45_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEU4187434.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            complement(12037..13704)
                     /locus_tag="VVL45_001263"
     CDS             complement(12037..13704)
                     /locus_tag="VVL45_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEU4187435.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDAVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            complement(13707..14072)
                     /locus_tag="VVL45_001264"
     CDS             complement(13707..14072)
                     /locus_tag="VVL45_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU4187436.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            complement(14229..14417)
                     /locus_tag="VVL45_001265"
     CDS             complement(14229..14417)
                     /locus_tag="VVL45_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEU4187437.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            complement(14533..14709)
                     /locus_tag="VVL45_001266"
     CDS             complement(14533..14709)
                     /locus_tag="VVL45_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEU4187438.1"
                     /translation="MDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLIN
                     DGMWRAENVTQDLI"
     gene            complement(14820..15017)
                     /locus_tag="VVL45_001267"
     CDS             complement(14820..15017)
                     /locus_tag="VVL45_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEU4187439.1"
                     /translation="MKEEKKAIVLGADNAYMDKVETTIKSLCVHHYNLKFYVFNDDLP
                     REWFQLMEKRLETLNSEIVNV"
     gene            complement(15300..16844)
                     /locus_tag="VVL45_001268"
     CDS             complement(15300..16844)
                     /locus_tag="VVL45_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEU4187440.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLETFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMNPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            17057..17359
                     /gene="gatC"
                     /locus_tag="VVL45_001269"
     CDS             17057..17359
                     /gene="gatC"
                     /locus_tag="VVL45_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEU4187441.1"
                     /translation="MKITQEEVTHVANLSKLRFSEEETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGIDRDRLFKNVPEKDNYYIKVPAILDNGGDA"
     gene            17359..18825
                     /gene="gatA"
                     /locus_tag="VVL45_001270"
     CDS             17359..18825
                     /gene="gatA"
                     /locus_tag="VVL45_001270"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEU4187442.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWNHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGIDPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            18825..20267
                     /gene="gatB"
                     /locus_tag="VVL45_001271"
     CDS             18825..20267
                     /gene="gatB"
                     /locus_tag="VVL45_001271"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEU4187443.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVKLEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMIAIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            20396..20956
                     /gene="efp"
                     /locus_tag="VVL45_001272"
     CDS             20396..20956
                     /gene="efp"
                     /locus_tag="VVL45_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEU4187444.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            20978..21367
                     /locus_tag="VVL45_001273"
     CDS             20978..21367
                     /locus_tag="VVL45_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU4187445.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            21360..21782
                     /gene="nusB"
                     /locus_tag="VVL45_001274"
     CDS             21360..21782
                     /gene="nusB"
                     /locus_tag="VVL45_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEU4187446.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(21817..23040)
                     /locus_tag="VVL45_001275"
                     /pseudo
     CDS             complement(21817..23040)
                     /locus_tag="VVL45_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT         6790 a         5198 c         4260 g         6792 t
ORIGIN      
        1 ataatcggca agtagaccta aaaatactgg ctagacatat tcatcgtctc aagaaaaaca
       61 gtctgattgg aaagtacaat acactcgttg tcttgatatc atctttcctg agttggataa
      121 aatcgttgga aagcattcag aatataccta ccaactcttg acacgctacc ctaatcctca
      181 gaaaaggatt gaggcaggat ttgataagct gatagaaatt aagcgattga ccgcttctaa
      241 aattcaggat atcctctcag tcgcacctcg ttctatcgga acaacatctc ctgctcgtga
      301 attcgaaatc atcaagcatt acaagaggct cattgacaag gcggaaacat gtatcaatga
      361 ctcgatggct gagttcaact cggtcatcac gacggttact gggattgggg atcgtttagg
      421 gcggtcattt tagccgagat tcgaaatatt catgcctttg ataatcctgc tcaattacaa
      481 gctttcgctg gactggattc ttctatttat cagtcaggtc agattgattt agctggaaga
      541 atggtcaaac ggggttcccc tcatctgcgg tgggcactca tacaagctgc caaagcatgc
      601 cctcgctttt cacctgcttc taaggcctat cttaagacta agttagaata aggaaaacat
      661 tacaatgtag ccatcatcca ccttgcaaaa aaacttatcc gaaccctgtt ttatatccta
      721 aaaaagagct gccatttgac gaacaaaaag tgatgtaatt tctgaaatta acacaggatt
      781 acctttgaaa aaatggactt tttcaagggc tttttgccgt gctctaaaat catcgccaaa
      841 tcatcaaatt gaaataagaa atagtttaga taaaactatt gacttttctt atagtttgtc
      901 ttttttctat cttccattat atcatacttt ttcagtctat ggtagctctc tttaaaaggc
      961 accgatactg ccgccacctc cgcctccaga gaagccacca ccagaacttc cacttccaga
     1021 agatacagag taggtgcttg ctgtatttgc gacactagca taatggctca tttgtgctgt
     1081 tgaatgataa aacgtactgt gccagccata agctacatag agattgatat ctggattttc
     1141 cacttgaatc tgatgaacct tcatcaaatg actaaccttg tccgcatagc caaataaggt
     1201 cgcatagacc aagaggcgat tccagaccac aatactttcc agttcagcct gatccaatcg
     1261 tgcaatctca cgcaacatat tttcaaaact ggtccagaga tagtagacct cagctcccgc
     1321 ttcatttaga acaccatcac gattatctag tcgaagcttc caataataga aaacagacaa
     1381 aactaaccct agaaaaccaa gtattggcaa agggaggtaa agatagccat gaacgtctaa
     1441 actgtacaag aacaaaccaa atccgataaa taggggcagg atagtcaagg cacccatacc
     1501 cacttgcaag gccttttccc caccagttaa aggacgataa taatctggga gcccccagaa
     1561 ggaaactcgt tttctcactc cttcttgcat ctggttcaat acctcttcaa aagaagattt
     1621 gagttgaagc cctcttgctt gaatccgttt ttcatcagaa actttggctc tacgataaag
     1681 actatcagat accttgtaat ccgcaaacaa attggaaaga gtttcttctt ttttacctga
     1741 aaaagctaga tttaggcagt ctttctcaaa gcttgacaaa ccatcttctt ttactagcct
     1801 caaaccaact gcatctcctt ctgaaatgat agagacattc ccacggtcta tcacatctag
     1861 caaggtagct tgaataagtt gatcaaaggt gaattttcca gctcccttga ccaagggact
     1921 cacttcctcc aaggaggtcg agtagactgc ttctgataaa accataggct ctaattccat
     1981 tggtggttca tagagacgat gatttttggc atatttgact gaaggagtgg tctttcttct
     2041 ataaataaaa tagaagcaga cactcaataa caaggagata gaaagtatcg aagggaggac
     2101 ccaagtaacg agttgtttac tctgatcttt ttctctaaca atcgagtctt ctatcttatt
     2161 aaactcttct aaacgattcc ctttcaagcc ctgatcccta gcgctagcaa aatcggtccg
     2221 agaccaatag gcatgcaact caactccacg cttagccgga agattgtcta aacggatagt
     2281 ataatcaagg ttactctttt caatcgttcc ctctctaaaa agtttccctg tatggaaaaa
     2341 gagtttctca gcccccttgt ctccccttac atgaaattca aacttttcaa tagactctga
     2401 actatctgtc agaggttgcc aatttaattc agcgatatca tcataaagga aaagtaaatt
     2461 ttttaagttc cagacgaggt caacttcaac tatgtcgccc tcctgacctg gattatagac
     2521 tctcacagta taaccatccg cttcttctgt tacttcgcta gtcacatctg ctagttctgc
     2581 accgtttttc gcagcctgaa cctttggatg agggtcaatg tcaaacccgc taggcatctt
     2641 accagcacgt ccaagtccca cgatttggcc cttaaagtcc tcctcaaact ggtaaactat
     2701 cttctgtcta aactctgccg tattatctgc atgaatatac aaatcccctt gataagagtt
     2761 tatcttgaaa tcaatggcaa aaacagagag tggaagaagg caaaacaagc ctaacaccag
     2821 taagaaaaaa gtttttttca tcaactaagc ccccttttta tctttcttat cattatagca
     2881 tcttttcgta agtaaaggct tacttatgtt gaaaaattac gcatttttcg ataaaatagg
     2941 agacagttat tttttaatag atcagaatag gagaaatcat gagaaaaata tacctatcta
     3001 ttttcacaag tctcttgctg atgctaggac ttgtcaatgt tgctcaagcc gatgaatatt
     3061 tacgcatcgg gatggaagca gcatatgctc cctttaactg gacccaggat gatgatagca
     3121 acggagctgt caaaatcgat gggaccaatc agtatgccaa cggatacgat gttcaaatcg
     3181 ccaagaaaat cgctaaggac ttaggtaaag aacctttggt tgttaaaacc aagtgggaag
     3241 gtctagtccc tgcccttact tctggtaaga ttgacatgat tatcgcaggt atgagtccaa
     3301 ctgcagaacg caaacaagaa attgcctttt cgagcagtta ctacactagc gaaccaattt
     3361 tgcttgtcaa aaaagattct gcctacgcaa gtgctaaatc tttggatgac tttaatggtg
     3421 cgaaaatcac ttctcaacaa ggtgtctacc tctacgactt gattgcacaa atcccaggtg
     3481 ctaaaaaaga aacagccatg ggagacttcg ctcaaatgcg ccaagctctt gaggctggtg
     3541 tcattgatgc ttatgtttct gaacgtccag aagcactgac tgctgaagct gcgaactcta
     3601 agttcaagat gattcaagta gaacctggtt tcaaaactgg ggaagaagat acagctatcg
     3661 ctatcgggct tcgtaaaaat gacaatcgta ttagccaaat caatgccagc attgaaacca
     3721 tttcaaaaga tgaccaagtt gccttgatgg atcgtatgat caaggaacaa cctgccgaag
     3781 ctacaacaac tgaagagact agcagtagtt tctttagcca agtcgctaaa attctttctg
     3841 aaaactggca acaactcttg cgtggtgctg gtatcactct tttaatctct atcgtcggaa
     3901 ccatcatagg tctcattatt ggacttgcca ttggtgtctt ccgtactgct cctctctctg
     3961 aaaacaaagc catttatggc cttcaaaaac tagtcggctg ggttctcaat gtctacattg
     4021 aaattttccg tggtacgcca atgattgttc aatcgatggt tatctactat ggaactgccc
     4081 aagctttcgg gatcaacctt gaccgtacac tggctgctat cttcatcgtt tcaatcaata
     4141 ccggtgccta catgactgaa atcgtccgtg gtggtatcct agcagttgac aagggacaat
     4201 ttgaagctgc gactgctctt ggtatgaccc ataaccagac catgcgtaag attgtcctac
     4261 ctcaggtagt ccgtaacatc ctacctgcaa ctggtaatga atttgtcatc aatatcaaag
     4321 atacatctgt attgaacgtt atctctgttg tcgaacttta tttctcagga aataccgtgg
     4381 caacacaaac ctatcaatac ttccagacat ttacaatcat cgccgtgatt tactttgtcc
     4441 tcaccttcac cgtaacacgt atcctacgat ttatcgagcg ccgaatggac atggatacct
     4501 atactacagg tgctaaccaa atgcaaacgg aggatttgaa ataatgacac aagcaatcct
     4561 tgaaattaaa cacctcaaaa aatcctatgg acaaaacgaa gtgctaaaag acatttcact
     4621 cactgtccac aagggagagg tcatctctat catcggaagc tctggaagcg gaaaatcgac
     4681 cttcctacgc tccattaacc tacttgaaac accaactgat ggacaaatcc tttatcatgg
     4741 acaaaacgtc ctcgaaaaag gctatgacct cacgcaatac cgtgaaaagt tggggatggt
     4801 tttccaatcc tttaacctct ttgaaaatct caatgttctt gaaaacacaa tcgtcgctca
     4861 gacaactgtc ctaaaacgcg aacgcacaga agctgaaaag attgccaaag aaaacctgga
     4921 aaaggtcggc atgggagaac gctactggca agccaaacca aaacaactct caggtggtca
     4981 aaaacaacgt gtggccatcg ctcgtgccct ctccatgaat ccggacgcta ttctctttga
     5041 tgaaccaaca tcagctctcg atccagaaat ggttggagaa gtcctcaaaa tcatgcagga
     5101 cctggctcag gaaggcttga ccatgattgt cgtaacccat gaaatggaat ttgcccgtga
     5161 tgtctctcac cgtgttatct ttatggataa gggcgtgatc gctgaagaag gtaaaccaga
     5221 agacctcttc accaatccta aagaagaccg aacaaaagag ttccttcaac gctatctcaa
     5281 ataaaaagaa aaggctgcat caaccgtgca gtctttttgc tgtcctcaaa atgaaaaggc
     5341 agattctcta tctctactac ttgaaggtca agaatccgcc gttatccaaa gattaatctt
     5401 caaatttttc ataatttttt tcatagaaat caattaagcc tagggctgtt tcaaatgaaa
     5461 tattttttac ttttgcacga ccctgcgcca gagcaatgat agacatttca cgcgcattcg
     5521 tttctttaga gatacggtag cctgtgattt tcttatcacg aacccagcca acaactgatt
     5581 ctactttttc aaagtttgac ttagccatgt ttttctccta ttttcttctt tataatattt
     5641 aattgtatac gtttttacta cttttgtcaa taaaaaaaac atatattcaa cgaaaataat
     5701 tagttcagaa ttttcttact tcctttttaa atccgataat atataggttt tgctagcctc
     5761 tacaccataa atatttgcta ataaaactac tgtattatta ccctcttaag gtgcgaaatt
     5821 attcaagttc ttaaaaaatg cttctctatt tccgtaagat agcttatttc ttcgatttaa
     5881 gaactgaaga tgatagttct aacctttaga aatccaagcc ttcactagct caaaaactcc
     5941 tatctttctt cctcaataaa tcatctgaga gataggtatt ccttatgtcc ttcaaattca
     6001 tcactccttt ggatattgag gtgctagcct agaagcagac acccgattat ctaaaccatc
     6061 cttaatagag atgacaagac agataagctc agttaattac ttatatatct ttttaatata
     6121 gtatatttgt caacaaaaag aaatcaaatc ataccttata ctcaatgaaa atcaaagagc
     6181 aaactaggaa gctagccgca agttgctcaa aacactgttt tgaggttgca gatagagcta
     6241 acgtggtttg aagagatttt cgaagagtat taaagacagt gtgactatgc ttgtcaatag
     6301 ataggaaatc aaaatgaagg tatgatattt ttttattagt cctcagacaa gcatattata
     6361 aagcgtttca ttaccattta agttaatata agctggatca aaaccttcca ttctacgaat
     6421 caaacctgca taatcatgct tatctgctaa agcgatccca attaatactg ggcctgttcc
     6481 cttgctagct cgtttgatat actcaaaacg tgtgatatca tcatttggcc ccaggatatc
     6541 atttacaaac tcacgcaaag ctcctggacg ttgtgggaaa ttgaccacaa agtaatgttt
     6601 gataccatca taaatcaagg cacgctcttc catttctggc atacggttga tatcattatt
     6661 tcctccagaa atgatacaac aaatggtttt ccccttaata tattcagcta aaacctctaa
     6721 agaggcgata ctagccgctc cagcaggttc tgcgactatc ccttgcttag agtaaaggtc
     6781 aatcaaggtt tcagaaatca atccctcatc gacacctacc aaagttttaa tatgttgacg
     6841 agttgcttca taggtcaact gacctacctt ttgcacagca atcccatcag caaatttatc
     6901 aatttccttg agttttactg gacctccagc ctcaaaggca gctttcatgg aacgcgctcc
     6961 attcgcctct actccgataa cctcaatctc tggacttgtt tccttgatat aggtagaaac
     7021 cccggcaatg agaccgccac caccaacagg aaccaagaca gcatcaaaat caatcgattc
     7081 ttttcgagct tcttctaaaa tctcataagc aactgttcct tgacctgctt gaacatgagc
     7141 atcatcaaaa ggatcaataa aggtacgatt ttcagagact gtaaattctt gagctgcttt
     7201 ggctgaggca tcaaaggtat ctccaactag tttaatagtt acaaaatccc caccaaaaaa
     7261 gcgaacctga ccaatctttt gttgtggcgt agtaatgggc ataaagatag tagcaggaat
     7321 tttcatttca ttacaagtat aggctactcc ctgcgcatga tttcccgcag aagcgcagac
     7381 taccccacgt tcacgttctt ccttgctgag ctgggaaatg gcataatagg caccacgaat
     7441 tttaaaggag cgaacacgct gggcattttc ttttttcaaa taaatcttag caccatactt
     7501 ctccgataaa taatgatcgt aatccagtgg agtattcaca accacaccgt tcaagacctt
     7561 gtgagccttg attatatctt ttgaacttaa catataaaac ctctttttct ataaaaaaca
     7621 gtcactaatg actgtttttt agttattcat ctccgaaaac ttcagtaaca ctttcatggt
     7681 atttatgagc catacgaatc gaaaactctt taggaagttc ataacgattt gtggaaatcc
     7741 aatcatataa ccattctacc aaactttctt cactatcatt acgatggaag ataaaatagt
     7801 ttaaaatttc tttatattct acatcattca aatccaacgt gtctacaaat tgactacgag
     7861 gaacaaaatt agaatctaat tttcttagtt gatctgtcaa ttctcccaat tgtaaatcca
     7921 actgaaaaaa tagtttttgc agttgcctat cagtttcatc acatcctgtc ttgatagaaa
     7981 acaaattggt aatattcata tgatttcaac ccctaaataa gtacatctaa tatcccacga
     8041 atagctgaag ctaaagtttt cgcagtccag tcatttaaac ccgattcttt caatccagat
     8101 gcaataattt gctctatttg atataaaccg ccatctatcg tctcatttat tctgcgaaga
     8161 ggaccttcaa tataggataa agcttctccg aatctaacag ctgatttagg tcctaatgat
     8221 ttaccaacta tatttgttaa tgttttttat ttctcaaaat ccatttcgct atataacgag
     8281 cagatttagt cgcaccagct ctctcaaacg gagaatgaat ttcaagacct tcttctctag
     8341 ctatttgtgc caatttgaag aattgttcaa agttcatatg caacctacca tcttcaaaat
     8401 tagcatcatc ataaatgtta attacagcat tttcatcata cgaccaatct gctttatttt
     8461 ggtctgctag cactattacc gacggtgtaa ttacattata caactagaac caataataac
     8521 gatgcaattt ttctcataat ctttttcata aaatcatctc cagcgctata tatttctaat
     8581 tagttataga ttttgaatgc atcatcgtcg tttttaccaa cgaatggcat tgctttacgc
     8641 aattctgcac caactttttc aatttcaagg ttagctgctt gttcacggta agcagtcaat
     8701 tttggacgtc cagctttata gtcatttaca aagtcatttg caaatttacc attttggatg
     8761 tctgccaaga cagccttcat attttcttta acttgttcag tgattacacg tggacctgat
     8821 acatagtcac cgtattcagc agtgtttgaa atagattgac gcattttctt gaatccacct
     8881 tcgtagatca agtcaacgat caatttcatt tcgtgaagaa cttcaaagta agccaattct
     8941 ggagcgtaac ctgcttctgt caagacttcg aaacctgctt cgataagggc agtcaaacca
     9001 ccacaaagta cagcttgttc accaaacaaa tcttcttcag tttcttcttt gtaagttgtt
     9061 tcaagaagac ctacacgagc cgctccaaca cctttacacc agtccatagc aatgttttta
     9121 gcatttcctg ttgcatcttg gtatactgca taaagagctg gaacaccaaa tccttcttcg
     9181 taagtacgac gtaccaagtg tcctggtcct ttaggagcac acatgaagac atctacatcc
     9241 gcaggaactt tgataaattc aaagtggatg ttgaaaccat gggcaaatcc aactgcgttt
     9301 ccagcttcca agtttggagc gatttctgct tcgtacaatt cttgttgaat ttcgtctggc
     9361 gccaagatca tgataacatc agccaactta gtagcttctg ctactgtgta agtatcaaat
     9421 ccatcttctt ttgctttatc aaaagattta cctggacgta caccgataat aacgtcacga
     9481 cctgaatcac gcaagttttg agcatgcgca tgcccttgtg aaccataacc gataacggcg
     9541 atttttttac cgtcaagtgc tgctacttta acatcttttt catattccat ttgaactgtc
     9601 atagcttttc tctcttttct attatttatt gccttttagg ctggtttaat aaatttaagt
     9661 tggattttta atcgcgggta aatccagttg cacccgttcg agcaatattg cgaataccgt
     9721 atgggcgaat gactcgcaat agggcttcgc tcttttctgc atttcccgtc atctgaatgg
     9781 taatcgagct tggcgctacg tctactactg ttgcacggaa aggttgaata atcgctaaaa
     9841 tctcagctct cttctcagct ggcgctgaca tcttaaccaa aatcacctcg cgctccaaat
     9901 gaggcttgtc tgtaatatct cgaatgcgaa tcacatcaat ctgacgattg agctgtttga
     9961 tgatttgctc cacttcatca tgagaagcaa catcaataat aatagtgata cgcgatacat
    10021 tcggatcttc tgttgctcca acagagatgc tttcaatatt aacctgacga cgagatagga
    10081 cacctgtaaa gcgattgagg actcctgaac gattttgtag ttttgctgtt aacattctac
    10141 gcatggaact gcacccccaa catctcatga ttactcttac cagccggtac cattggtaac
    10201 acctgttcct tacgagaaat atctacctca attagcatag gaacatcctc agtgatgact
    10261 tcaaggtctt gagccaaggt ctcaggattg tcaaacttat agtttttaat accataagcc
    10321 tgcgccatca attggaaatc aggaagggta tcaaagaccg actctgatgt tctgccttca
    10381 tagaaggatt cctgccactg gcgaaccatt ccaagtgaat gattgttcag cataaccacc
    10441 ttgattggca ccttgtaaat attcaaaata gccaactcct ggttggtcat ttggaaacca
    10501 ccatccccaa caaacaagac tacttcctta tctgggttag caattttagc accgattgct
    10561 gctggaattc caaagcccat tgttcccaaa ccacctgaag tcactaactg acgttcattt
    10621 tggtagggat aatactgagc tgtccacatt tggtgttgac caacgtctgt taccacaatg
    10681 gcatctccat tcgtcaattc accaattcgt tcaataactg cttgcggttg aaccacacgc
    10741 tctttcttat cataagaacg aacacgattc ttgtctttag tgactttctc aatccacttt
    10801 tcagtgttgt tgtgaactgt tggttctgct agcaacattt gcaaggcctt cttagcatct
    10861 ccaactacag gaatgtctgc actgataatc ttgccaatct cagctgggtc aatatcaatg
    10921 tgggcaacct tagcattctt agcgaaagtc ttaggattcc ccgtcaaacg gtcatcgaaa
    10981 cgagaaccaa tactaatcat aaagtccgct tccgtcatag caatatttgc tgcgaatgac
    11041 ccgtgcatgc ctcccattcc aagaaagagt gggtgactcg ttgcaatcgt tccttgtccc
    11101 aaaagactgg ttaccactgg aatttgatag cgttctgcaa attcatttag ttccgtagca
    11161 gcctcagcat aactaattcc accaccagct aacaagactg gctttttagc cttggacaat
    11221 tgcttcaaga ttttcttgat ttgcatatca ttcggctcaa gagtcggctg ataacttggt
    11281 aaattcactt ctggtgaata aatgaagtct gtttctaaag cagatatgtc ttttggtagg
    11341 tcaattacaa ctggccctgg acggcctgta gttgcgatat ggacagcttc cgtaatgata
    11401 cgcggaatat cagctgtctc acgaacttgg taattgtact tagtgattgg catggtaatt
    11461 cccacgatgt ctgcctcctg aaaggcatcc ttcccaatcc ctgctcgcgc cacctgacct
    11521 gtaaagacca aaaggggaac gctatcgctc atggcatccg caatccctgt aatggcattt
    11581 gttgctcctg gtccactagt gacgacggca acacccaact ttccagttga tttggcataa
    11641 ccttcagctt catgcaaaca accttgctca tggcgcccta gaatgtggcg aatgccttta
    11701 aaattatata tcgcatcata aaaaggcaaa accgcaccac caggataacc aaagatggta
    11761 tcaactccta aatcacgaag tgtttccaaa actaggtccg accccgtctt aggagattct
    11821 aaactgattt tctccattat tcccctttct ttcctcttaa aaataacttg ttactatcat
    11881 accatttttt caaaattttt caagataaaa gaagaaattt tctgaatttt ctattttaac
    11941 gtttatttat gaatgttatt tttgttttta ttaaaaagat accgatttca tttactaaaa
    12001 agaaaaaaga actgatttct cagtccttca ttaatcttat tccacactaa ataggtatgg
    12061 gtaaacaggt tgttgacctt ggtgaatctc gacttcaacg tcttcgaatt cttctacgat
    12121 ttcttgagcg atttcattgg caagttcttc gcttccgtct tcacctacat agaaggttac
    12181 gatttcactg tcttcatcca acatatgttt caaggtttca gtcaatgttt ggtgcatatc
    12241 agggtttgac acaagaattt ttccatccac catacctaaa ttatcgtttt catggatttc
    12301 taagccatcg atcgttgtat cacgcacggc tgttgtgacg cttccgctaa cgacatcgct
    12361 aagagcagct gtcatacgct cttggttttc ttcaatggac ttgcttggat caaaggcaag
    12421 aagacttgtc ataccttgag gaagagtgcg agcctctacc actaccgctg gttgctccaa
    12481 aacttctgcc gcagattgag ctgccatgaa gatgttcttg ttgtttggca agaagatgat
    12541 gttacgggca ttaacctgtt caacagcctt gataaagtct tctgttgaag ggttcatggt
    12601 ttgaccgcct tcgataacat aatccacgcc ttgagaacag aagatatctg ctagaccttt
    12661 accagccacc acagcaatca aagcatactc tttttcttca gccgacttga taacttgagt
    12721 agcttctttc tcaacctgtg cttcgtgttg gttacgcata ttgtcaactt ttaccttgac
    12781 caagctacca tatttgagac cttcttgcat aacaagtcct ggatcttctg tatgaacatg
    12841 gactttgaca atttcatcat cgttaacaac aaggagagaa tctccaagct catccaagta
    12901 gttacggaat tcatcgtagt caaaatcttt agcataggtt ggaccttgct taagagctac
    12961 catgatttca gtacagtaac caaacgtgat gtcctcagtc gctacgtgac cagctacaga
    13021 cttatgatgc tctacattga tcatctcact catgttggca ggagtcgcta caaagtcctc
    13081 agatgcaata tattcgccag taagggctga aaggaaacct tcgtagatga agaccaatcc
    13141 ttgaccacct gagtccacaa cgccaacttc tttcaatact ggaagcatgt ctggtgtttt
    13201 agctagagct gttttagcac cttccaaggc tgcgcgcatg acttcaacag cgtcatctgt
    13261 ttgctcagct tttttcttag caccgatagc agctccacga gaaactgtta aaatcgttcc
    13321 ttcaacaggt ttcatcactg ccttataggc aacttccaca cctgattgga aggccagagc
    13381 caagtcttga cctgttaact cgtctttatc cttgatagct tgggaaaatc cacggaaaag
    13441 ctgagacgta atcactcctg agttcccacg cgcacccatc aaaagccctt tggcaagaat
    13501 gctcgctact tctccaactg tagaagctgg cttgtctgca acttctttag caccattttc
    13561 aatggtcatt cccatatttg tcccagtatc tccatctgga actggaaaga cgtttaatga
    13621 attgacatat tcagcttgct tattcaagcg agttgatgca gcctgcacca tttcttgaaa
    13681 taagctagta gtaatttttg acacggttat tctcctacaa ctttgatatt ttgaatgtag
    13741 acatttacag tctgagcagt aattccaagc tggttttcca agctaaaggc aacacgctct
    13801 tgaatgtttt ttgacacttc actaatcttt gttccgtagc ttaacacggt atatacatca
    13861 actgcaatac tgccatcttc ggctgccttt acgacgacac ctttagaata attttcctta
    13921 cctagcaggg cttggaaatt atctttgagg gcatttttac tagccatacc gaccacacca
    13981 aaaatctcag ttgctgctcc acctacgacg gttgcaatca cttcatctgt tagttcgatt
    14041 tgaccatctt ttgtattaat ttttacagtc atccttttta cctcaactag ttgatactct
    14101 attttatcat atttcagccc aagtgtaaaa gcagaatgat gtatcagcgg atatttactc
    14161 tatttttcaa acgatgttat acccacaata aaagaaaaaa gaccctaagg tctcctttgc
    14221 ttttattatt aaacgcgttc aactttacct gatttcaaag cacgagctga agcccaaact
    14281 tttttaggtt taccatcgat aagaacagta actttttgaa ggtttggttt tacggcacgt
    14341 tttgtttggt tcatcgcgtg tgaacggttg tttcctgata cagtcttacg acctgtaaag
    14401 taacatactt tagccattgt gttttcctcc tattagatct aatatagcgg atgtgctagc
    14461 accacatacc gtactatgtt atcacatttt cttgtttttt gcaagggaat tggaagattt
    14521 tttatttgtg tcttaaatca ggtcttgcgt gacattttct gctctccaca tgccatcgtt
    14581 gattaacaga acaccagaat taaaattatg tgtataaaaa tcatctctaa ctgcagctaa
    14641 gggtatagcc gtcaagtcca aatcccacag ctcatctatc gattttctta caacaatatc
    14701 tgaatccaaa tacagtacac gagactcgct tacatacttt ggaataaaat acctaaaaaa
    14761 gccgcatatg aaagtccctc aaaggggaga cgataacctt tcagaatatt actgtcaatc
    14821 taaacattca caatctcact attcaaagtc tctagtcttt tttccatcaa ttggaaccat
    14881 tctcgcggaa ggtcatcatt aaaaacataa aacttaagat tataatgatg aacacaaaga
    14941 gattttattg ttgtttcaac tttatccata taagcattat ctgcacctaa gacaatcgct
    15001 tttttctctt ctttcacttt ttatctcatt tctttttatt cccatcatat tattcccatc
    15061 atatgtttcc catcatatgt ttctacgtaa ccattatttt cgcctattcg ttcgtaaaac
    15121 cataccagtg gagattttag atgaagtccc attacggttt acaattttta cattacgaca
    15181 cggagtttta caaatcgatt tcatttgcca aacgtagtta gtgaggcagt tagctagttc
    15241 gccaaatagc gactagcgtc caacaatttg gaactttagt tccaattgtt ggtactgagt
    15301 cacatcttct cctctaactc tacgtctgga tacttgtccg caaaccagcg gagggcaaag
    15361 tcattttcaa agagaaagac tggttggtca aaacggtctt tggctaagat attgcgactt
    15421 gacgacatcc gttcatccaa gtcctcaggc ttgatccaac gaacggtctt tttacccatt
    15481 gggttcataa ctacttccgc attgtactcg ccttccatgc ggtgtttaaa gacttcaaac
    15541 tggagttgac ctacagcgcc tagcatgtac tcacctgttt ggtaattctt ataaagctga
    15601 acggctcctt cttgcaccaa ttgctcaatc cccttgtgga aggatttttg cttcataaca
    15661 ttcttagcag aaactttcat gaaaatctca ggtgtaaagg ttggcagggg ttcaaattca
    15721 aacttgtttt ttccaaccgt caaggtatcc ccaacctgat aagtaccggt atcgtaaacc
    15781 ccgataatat cacctgccac ggcattggtc acattctcac gactctccgc cataaactgg
    15841 gtaacattag atagtttagc ccccttacca gtacgaggga gattgacact catgccgcgc
    15901 tcaaattcgc cagatacgat acggacaaag gcaatacggt cacggtgacg agggtccatg
    15961 ttggcttgga ttttaaagac aaagcctgag aaatccttgt cataaggatc cacaatttca
    16021 ccgtctgttt tcttgtgacc atgtggttct ggagcaaact tgaggaaggt ttcaaggaag
    16081 gtctgcacac caaagtttgt cagggctgaa ccgaaaaaga caggcgtcaa ttctccagcc
    16141 agaatagctt cctctgaaaa ctcattcccg gcttcattta aaagctcaat gtcatccttg
    16201 acttgctcgt agaaaggatt gctaccaaag agtttgtccc cgtcttctag actggcaaaa
    16261 cgctcatccc ctttgtaaag ctctaaacgt tggttataga ggtcatacaa gccctcaaag
    16321 gctttcccca tcccgatagg ccagttcata gggtagctag caatgcccaa gatttcttcc
    16381 aattcttgca agagatccaa aggctcacga ccgtcacggt ccagcttgtt cataaaggta
    16441 aagactggaa tgccacgatg tttcacaacc tcaaacaatt tcttggtttg agcctcgatc
    16501 cccttggcag agtccacgac catgaccgca gcatccaccg ccatcaaggt acgataggta
    16561 tcttctgaga agtcctcgtg ccctggcgtg tctaagatat tcacgcgctt gccgtcgtag
    16621 tcaaattgca taacagatga agtaacagaa atcccacgtt gcttctcgat atccatccag
    16681 tcagatttag caaaagtccc tgttttcttc ccttttaccg taccagcctc acgaatctca
    16741 cccccaaagt agagtaactg ctcagtgatg gttgttttcc ccgcgtccgg gtgggagata
    16801 atggcaaagg tacgacgttt cttaatttct tcttgaatat tcataagttc tctttctttg
    16861 attctctatt tttcttgttt caatagctga gaatgatttt tacattggat tttaccattc
    16921 ctttcaacac tccattatat cggattttag catttttttc aatttctatt tcttttcact
    16981 tccccctccc ttatttatag gaaaatatgg taaaatagaa cagactaaaa atcatcattt
    17041 cacgaaagga tgcaagatga aaattacgca agaagaggta acacacgttg ccaatctttc
    17101 aaaattaaga ttctctgaag aagaaactgc tgcctttgcg accaccttgt ctaagattgt
    17161 tgacatggtt gaattgctgg gcgaagttga cacaactggt gtcgcaccta ctacgactat
    17221 ggctgaccgc aagactgtac tccgccctga tgtggccgaa gaaggaatag accgtgatcg
    17281 cttgtttaaa aacgtacctg aaaaagacaa ctactatatc aaggtgccag ctatcctaga
    17341 caatggagga gatgcctaat gacttttaac aataaaacta ttgaagagtt gcacaatctc
    17401 cttgtctcta aggaaatttc tgcaacagaa ttgacccaag caacacttga aaatatcaag
    17461 tctcgtgagg aagccctcaa ttcatttgtc accatcgctg aggagcaagc tcttgttcaa
    17521 gctaaagcca ttgatgaagc tggaattgat gctgacaatg tcctttcagg aattccactt
    17581 gctgttaagg ataacatctc tacagacggt attctcacaa ctgctgcctc aaaaatgctc
    17641 tacaactatg agccaatctt tgatgcgaca gctgttgcca atgcaaaaac caagggcatg
    17701 attgtcgttg gaaagaccaa catggacgaa tttgctatgg gtggttcagg tgaaacttca
    17761 cactacggag caactaaaaa cgcttggaac cacagcaagg ttcctggtgg gtcatcaagt
    17821 ggttctgccg cagctgtagc ctcaggacaa gttcgcttgt cacttggttc tgatactggt
    17881 ggttccatcc gccaacctgc tgccttcaac ggaatcgttg gtctcaaacc aacctacgga
    17941 acagtttcac gtttcggtct cattgccttt ggtagctcat tagaccagat tggacctttt
    18001 gctcctactg ttaaggaaaa tgccctcttg ctcaacgcta ttgccagcga agatgctaaa
    18061 gactctactt ctgctcctgt ccgcatcgcc gactttactt caaaaatcgg ccaagacatc
    18121 aagggtatga aaatcgcttt gcctaaggaa tacctaggcg aaggaattga tccagaggtt
    18181 aaggaaacaa tcttaaacgc ggccaaacac tttgaaaaat tgggtgctat cgtcgaagaa
    18241 gtcagccttc ctcactctaa atacggtgtt gccgtttatt acatcatcgc ttcatcagaa
    18301 gcttcatcaa acttgcaacg cttcgacggt atccgttacg gctatcgcgc agaagatgca
    18361 accaaccttg atgaaatcta tgtaaacagc cgaagccaag gttttggtga agaggtaaaa
    18421 cgtcgtatca tgctgggtac tttcagtctt tcatcaggtt actatgatgc ctactacaaa
    18481 aaggctggtc aagtccgtac cctcatcatt caagatttcg aaaaagtctt cgcggattac
    18541 gatttgattt tgggtccaac tgctccaagt gttgcctatg acttggattc tctcaaccat
    18601 gacccagttg ccatgtactt agccgaccta ttgaccatac ctgtaaactt ggcaggactg
    18661 cctggaattt cgattcctgc tggattctct caaggtctac ctgtcggact ccaattgatt
    18721 ggtcccaagt actctgagga aaccatttac caagctgctg ctgcttttga agcaacaaca
    18781 gactaccaca aacaacaacc cgtgattttt ggaggtgaca actaatgaac tttgaaacag
    18841 tcatcggact tgaagtccac gtagagctca acaccaattc aaaaatcttc tcacctactt
    18901 ctgcccactt tggaaatgac caaaatgcca acactaacgt gattgactgg tctttcccag
    18961 gagttctacc agttctcaat aaaggggttg ttgatgccgg tatcaaggct gctcttgccc
    19021 tcaacatgga catccacaaa aagatgcact ttgaccgcaa gaactacttc tatcctgata
    19081 accccaaagc ctaccaaatt tctcagtttg atgaaccaat cggatataat ggctggattg
    19141 aagtcaaact agaagacggt acgaccaaga aaatcggtat cgaacgtgcc cacctagagg
    19201 aagacgctgg taaaaacacc catggtacag atggctactc ttatgttgac ctcaaccgcc
    19261 aaggggttcc cttgattgag attgtatctg aggcagatat gcgttctcct gaagaagcct
    19321 atgcttatct gacagccctc aaggaagtta tccagtacgc tggcatttct gacgttaaga
    19381 tggaggaagg ttcgatgcgt gtggatgcca acatctccct tcgtccttat ggtcaagaga
    19441 aattcggtac caagactgaa ttgaagaacc tcaactcctt ctcaaacgtt cgtaaaggtc
    19501 ttgaatacga agtccaacgc caggctgaaa ttcttcgctc aggtggtcaa atccgccaag
    19561 aaacacgccg ttacgatgaa gcgaataaag caaccatcct catgcgtgtc aaggaagggg
    19621 ctgctgacta ccgctacttc ccagaaccag acctacccct ctttgaaatt tctgacgagt
    19681 ggattgagga aatgcggact gagttgccag agtttccaaa agaacgtcgt gcgcgttatg
    19741 tatctgacct tggtttatca gactacgatg ctagtcagtt gactgctaat aaagtcactt
    19801 ctgacttctt tgaaaaagct gttgccctag gtggtgatgc caaacaagtc tctaactggc
    19861 tccaagggga agtcgctcag ttcttgaatg ctgaaggtaa aacactggaa caaatcgaat
    19921 tgacaccaga aaacttggtt gaaatgattg ccatcatcga agacggtact atttcatcta
    19981 agattgccaa gaaagtcttt gtccatctag ctaaaaatgg cggtggcgcg cgtgaatacg
    20041 tggaaaaagc aggtatggtt caaatttcag atccagctat cttgatccca atcatccacc
    20101 aagtctttgc cgataacgaa gctgctgttg ccgacttcaa gtcaggcaaa cgtaacgccg
    20161 acaaggcctt tacaggattc cttatgaagg caaccaaagg ccaagccaac ccacaagttg
    20221 cccttaaact acttgcacag gaattggcga agttgaaaga aaactagaca gaacaaaacc
    20281 agccctaagg ttggtttttt cttctctacc aactcccaat aactattttg gctttatttc
    20341 cagagtattt tatggtaaaa tgaagagtaa taatatttat taaagaggta aaaacatgat
    20401 tgaagcaagt aaattaaaag ctggtatgac ctttgaaaca gctgacggca aattgattcg
    20461 cgttttggaa gctagtcacc acaaaccagg taaaggaaac acgatcatgc gtatgaaatt
    20521 gcgtgatgtc cgtactggtt ctacatttga cacaagctac cgtccagagg aaaaatttga
    20581 acaagctatt atcgagactg tcccagctca atacttgtac aaaatggatg acacagcata
    20641 cttcatgaat acagaaactt atgaccaata cgaaatccct gtagtcaatg ttgaaaacga
    20701 attgctttac atccttgaaa actctgatgt gaaaatccaa ttctacggaa ctgaagtgat
    20761 cggtgtcacc gttcctacta ctgttgagtt gacagttgct gaaactcaac catctatcaa
    20821 aggtgctact gttacaggtt ctggtaaacc agcaacgatg gaaactggac ttgtcgtaaa
    20881 cgttccagac ttcatcgaag caggacaaaa actcgttatc aacactgcag aaggaactta
    20941 cgtttctcgt gcctaatctc tagaaagagg tcattctatg ggaattgaag aacaacttgg
    21001 cgaaatcgtt atcgccccac gtgtacttga aaaaatcatt gctatcgcta ctgcaaaggt
    21061 agagggtgtt cactcttttt caaacagatc agtgtctgat accctttcaa aactttcact
    21121 cggccgtggc atttatctta aaaacgtgga cgaagaactc acagcagata tctatctcta
    21181 ccttgagtac ggagtaaaag ttcctaaggt agcggttgct atccagaaag ctgtcaaaga
    21241 tgccgtccgt aatatggctg atgtagaact cgctgctatc aatattcacg ttgcaggtat
    21301 cgtcccagat aaaacaccaa aaccagaatt gaaagatcta tttgacgagg acttcctcaa
    21361 tgactagtcc actattagaa tctagacgcc aactccgtaa atgcgctttt caagctctca
    21421 tgagccttga gttcggtacg gatgtcgaaa ctgcttgtcg tttcgcctat actcatgatc
    21481 gtgaagatac ggatgtacaa cttccagcct ttttgataga cctcgtttct ggtgttcaag
    21541 ctaaaaagga agaactagat aagcaaatca ctcagcattt aaaagcaggt tggaccattg
    21601 aacgcttaac gctcgtggag agaaacctcc ttcgcttggg agtctttgaa atcacttcat
    21661 ttgacactcc tcagctggtt gctgttaatg aagctatcga gcttgcaaag gacttctccg
    21721 atcaaaaatc tgcccgtttt atcaatggac tgctcagcca gtttgtaaca gaagaacaat
    21781 aaggctcttt gtcaactgta gtgggttgaa aaaaagctaa gctcgagaaa ggacaaattt
    21841 cgtcctttct tttttgatgt tcaaagcgat aaaaatccgt tttttgaagt tttcaaagtt
    21901 tcgaaaacca aaggcattgc gcttgataag tttgatgaga ttattggtcg cttccagttt
    21961 ggcattagaa tagtgtagtt gaagggcgtt gacaatcttt tctttatctt tgaggaaggt
    22021 tttaaagaca gtctgaaaaa taggatgagc ctgcttaaga ttgtcctcaa taagtccgaa
    22081 aaatttctct ggttccttat tcgggaagtg aaacagcaag agctgataga gctgatagtg
    22141 gtgtttcaag tcttgtgaat ggctcaaaag cttgtctaaa atctctttat tggttaagtg
    22201 catacgaaaa gtaggacgat aaaatcgctt atcactcagt ctacggctat cctgttgaat
    22261 gagtttccag tagcgcttga tatccttgta ttcatgggat tttcgatgaa actgattcat
    22321 gatttggaca cgcacacgac tcatggcacg gctaagatgt tgtacaatgt gaaagcgatc
    22381 aagaacgatt ttagcattcg ggagtgaaac agtctgggag actgtttcag cctgagccta
    22441 ggaatttgaa agcgaagctg tttagccaag tcatagtaag ggctaaacat atccatagta
    22501 ataattttga cgcgacatcg gacaactcta tcgtagcgaa gaaagtgatt tcgaatgata
    22561 gcttgtgttc taccctcaag aacagtgatg atattgagat tgttaaaatc ttgcgcaatg
    22621 aagctcatct ttccctttgt aaaagcatac tcatcccaag acataatctc aggaagacaa
    22681 gaaaaatcat gtttaaagtg aaaatcattg agcttacgaa taacagttga agttgagatg
    22741 gaaagctgat gggcaatatc agtcatagaa atcttttcaa tcaacttttg agcaatcttt
    22801 tggttgatga tacgagggat ttggtgattt ttcttgacga gggaagtctc agcgaccgct
    22861 atttttgagc actgatagca cttaaagcga cgctttctaa ggaggattct agtaggcata
    22921 ccagttgtct caaggtaagg aatcttagac ggtttttgaa agtcatattt cttcaattgg
    22981 tttccgcact cagggcaaga tggggcgtcg tagtccagtt tggcgatgat ttccttgtgt
//
LOCUS       DAVHEX010000034        22911 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVHEX010000034 DAVHEX010000000
VERSION     DAVHEX010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22911
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.034"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(1..207)
                     /locus_tag="VVL45_001276"
                     /pseudo
     CDS             complement(1..207)
                     /locus_tag="VVL45_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(269..718)
                     /locus_tag="VVL45_001277"
     CDS             complement(269..718)
                     /locus_tag="VVL45_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEU4187447.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(720..1478)
                     /locus_tag="VVL45_001278"
     CDS             complement(720..1478)
                     /locus_tag="VVL45_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEU4187448.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAKPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(1593..3572)
                     /gene="pknB"
                     /locus_tag="VVL45_001279"
     CDS             complement(1593..3572)
                     /gene="pknB"
                     /locus_tag="VVL45_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEU4187449.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSAE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(3569..4309)
                     /locus_tag="VVL45_001280"
     CDS             complement(3569..4309)
                     /locus_tag="VVL45_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEU4187450.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDAE"
     gene            complement(4324..5637)
                     /gene="rsmB"
                     /locus_tag="VVL45_001281"
     CDS             complement(4324..5637)
                     /gene="rsmB"
                     /locus_tag="VVL45_001281"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEU4187451.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLLAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANNSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVEDRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(5630..6565)
                     /locus_tag="VVL45_001282"
     CDS             complement(5630..6565)
                     /locus_tag="VVL45_001282"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEU4187452.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEDLMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(6578..8974)
                     /locus_tag="VVL45_001283"
     CDS             complement(6578..8974)
                     /locus_tag="VVL45_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEU4187453.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLKSQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRSGRAEKAGQVLIQSYNPQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGSTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(9040..9354)
                     /locus_tag="VVL45_001284"
     CDS             complement(9040..9354)
                     /locus_tag="VVL45_001284"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEU4187454.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(9379..10005)
                     /gene="gmk"
                     /locus_tag="VVL45_001285"
     CDS             complement(9379..10005)
                     /gene="gmk"
                     /locus_tag="VVL45_001285"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEU4187455.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(10135..11748)
                     /locus_tag="VVL45_001286"
     CDS             complement(10135..11748)
                     /locus_tag="VVL45_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEU4187456.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            complement(11939..12193)
                     /locus_tag="VVL45_001287"
     CDS             complement(11939..12193)
                     /locus_tag="VVL45_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEU4187457.1"
                     /translation="MLLKFTEDAWADYCYWQNQDKKTLKRINKLIKDIQRDPFTGIGK
                     PEPLKYDYQGAWSRRIDAENRLIYMMDGDSVAFLSFKDHY"
     gene            complement(12197..12451)
                     /locus_tag="VVL45_001288"
     CDS             complement(12197..12451)
                     /locus_tag="VVL45_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEU4187458.1"
                     /translation="MEAVLYSTFRNHLKDYMKKVNDEFEPLTVVNKNPDEDIVVLSKS
                     EWDSIQETLRIAQNKELSDKVLRGMAQVRAGSTQVHVIEE"
     gene            complement(12689..13786)
                     /locus_tag="VVL45_001289"
     CDS             complement(12689..13786)
                     /locus_tag="VVL45_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEU4187459.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRTQMALENGADLVVELPFLVSVQAADFFGQGAMDILDRLGIDSLVFGTEEVRD
                     YQKIADLYTEKGAEMEKFVENLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLTYILMQARESDLPEAIHVLGFTEKGRQHLKSLKGQVSLVSRIGKEPWDA
                     MTQKADQIYQLGKPSIAEQNFGRVPIRIETN"
     gene            complement(13796..14542)
                     /locus_tag="VVL45_001290"
     CDS             complement(13796..14542)
                     /locus_tag="VVL45_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU4187460.1"
                     /translation="MIMATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLE
                     LACGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGK
                     YDFVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHE
                     NAEDFAMLWDTYEDEVPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQ
                     AGFKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            complement(14645..14998)
                     /gene="rsfS"
                     /locus_tag="VVL45_001291"
     CDS             complement(14645..14998)
                     /gene="rsfS"
                     /locus_tag="VVL45_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEU4187461.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYLVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            complement(15011..15511)
                     /locus_tag="VVL45_001292"
     CDS             complement(15011..15511)
                     /locus_tag="VVL45_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEU4187462.1"
                     /translation="MKTAFIIIDVQNVLVETGFQTKSLLEKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            complement(15512..16105)
                     /gene="yqeK"
                     /locus_tag="VVL45_001293"
     CDS             complement(15512..16105)
                     /gene="yqeK"
                     /locus_tag="VVL45_001293"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEU4187463.1"
                     /translation="MSYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSDQEFLVLIDRYQLDPDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEIHTVGAGQMTDLDKVIYVADYIEHNRAFPGVDVAREIASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKED"
     gene            complement(16105..16734)
                     /locus_tag="VVL45_001294"
     CDS             complement(16105..16734)
                     /locus_tag="VVL45_001294"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEU4187464.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKV
                     GTSYPVIWVDVPLMDISSSMVRDFLAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            complement(16786..17097)
                     /gene="yhbY"
                     /locus_tag="VVL45_001295"
     CDS             complement(16786..17097)
                     /gene="yhbY"
                     /locus_tag="VVL45_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEU4187465.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            complement(17311..18417)
                     /gene="yqeH"
                     /locus_tag="VVL45_001296"
     CDS             complement(17311..18417)
                     /gene="yqeH"
                     /locus_tag="VVL45_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEU4187466.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGIAKVAVWAPEGVAVVTRKAII"
     gene            complement(18420..19010)
                     /locus_tag="VVL45_001297"
     CDS             complement(18420..19010)
                     /locus_tag="VVL45_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEU4187467.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            complement(18962..19870)
                     /locus_tag="VVL45_001298"
     CDS             complement(18962..19870)
                     /locus_tag="VVL45_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU4187468.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESETVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLENHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFV
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            complement(19927..20487)
                     /locus_tag="VVL45_001299"
     CDS             complement(19927..20487)
                     /locus_tag="VVL45_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEU4187469.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            20626..21835
                     /gene="sstT"
                     /locus_tag="VVL45_001300"
                     /pseudo
     CDS             20626..21835
                     /gene="sstT"
                     /locus_tag="VVL45_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            21903..22856
                     /locus_tag="VVL45_001301"
     CDS             21903..22856
                     /locus_tag="VVL45_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEU4187470.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
BASE COUNT         6153 a         5405 c         4496 g         6857 t
ORIGIN      
        1 attcgctaat tgttttttag ggcgattgat ttttacttcc gtcacatcaa tcattatcgt
       61 gtcctcaaag ctgagaggag ttcttgaaat cgtaacacca ctttgaacaa gagttacttc
      121 aacccattgg ctccgacgga ttaagttgct ttcgtggata ccaaaatcag ccgcaatttc
      181 ttcataagtg cggtattctc gcacatatag aaagcgttat caatttattt atctcatttt
      241 tcagaaaatt cttttatttc tgtaaagtct acgatactcg atgtgttttt atataatgat
      301 agagtctgag aatcactgtt ccgctagcca ttccaataga gattaccaaa gccaacatga
      361 caaccaaggt cgcacttgcc agtgctttat tatagtcccc tgtcacaaaa aaggcagttg
      421 ttcggtagga gagataacct ggaaccagcg gtgccaaaat ggccaagata aagaccacag
      481 caggtgtctt ataaagaata cttaaaatct ggctgacaca agaaccaata atggctgcaa
      541 tgaaggtagc tacaatgaca ttggtcggtt ccttgagcaa gagatagatt agccagacag
      601 tcatgcccaa aatccctcca ggtaagagca tagaccgttg cacattgagt acgattaaaa
      661 aagtgataat ggcaagaaaa cttgctactg cttgtaataa aaaggttgtt agtgtcatat
      721 tagttcatca ataccaaggc gacagaagtt cctgccccta aagcgagggt aatgagcagg
      781 gattcaaaca tcttactcat accagagttt atgtggttgg tcataatatc acggaccgca
      841 ttggtcaagg caatacctgg tacaaacggc atgaccgcac cagctataat caaatctgcc
      901 gttgaaggaa aacctgtgta gcgagcccaa aactgggcaa ttatcccaaa gacaaaggct
      961 ccagcaaagg ctgtcacaaa gggaattcgg ataaattttt ccacatagag ggaaaaggca
     1021 aaaccaaata aggtcgccac tcctgcccca agtgcgtcgt agatatttcc gctaaacata
     1081 actgaaaaga aaggagcact aaaggtcgca gccagagtta cctgcaactt agtataggga
     1141 aggggtttgg cttgcaaggc cgtcaattgc ttaaaggctg tttctaagtc aatctgcccc
     1201 ccaactagct gacgagaaat ctggttcaca tcgcagactt tttcgatgtt ataagaagag
     1261 gaggtcacgc gcttcatgcg cgaaatattg gtattttcaa tagagaaaaa gatagcggca
     1321 ggcatggcaa ggacattgca atccacaatc ccctgcgaat gcgcgattcg aatcatggta
     1381 tcttctacac gatggatttc tgagccactt ttaaggagaa tagtccccgc tagcataatc
     1441 acatcaatga cggcatttaa ttcttttgat tcttccatgc tttcctcctt ttatcaactc
     1501 cctctattct atcacaaatc cggactcaaa aaaaatcttt gccatgaaat catgacaaag
     1561 attgattact cattttgatt atccatctgc ttttaaggag tagctgaagt tgttttaggt
     1621 ttgtagattg aaatcttgac tctagtctta ttgaggtcta ccttttcacc tgctctagga
     1681 ctttgttcaa caaccatgcc ttctgcacta cctgcaggcg ctgtcgtcac ttctacaact
     1741 tctatattag cttccttaat cccaacaatt tgaatcaaat tgttcttagt aaactccaag
     1801 ctagaaccaa tgtaactcgg catggcaaca cttgtaactt ttttagctac tgtcaagaca
     1861 atttgagtag gtttactcac atcataagtc gttccggcac ctggactttg tttcataatc
     1921 gttcctggtt cgctttcgct ggactcttct tcctctatct taatcaaatt ctcaggaacc
     1981 ttcttctgct tgagttctga gattacttct gtagagttcc gtccaatata gttccctaat
     2041 tgaatcgtcg tagctttttt agctactgtc aaaacaattt gagttgcctt gctcaagtca
     2101 taggtcgtac cttctggtag actttgcttc aggaccgttc cagcctcact ctcattcgac
     2161 tcttcttcct caattttaat caaattatct ggaacttttt tctcttttaa ttccgcaatg
     2221 acatcagagg atttccgacc gacataatta ctaatttgga aagattgctt gcctgatgag
     2281 acaaccaaat tgattttcgt tccttctttt cgaccagttc cagcgccagg atctgtacgg
     2341 ataatccgcc cttcttccac cttttcacta gcctctgtct tctcctcacc aatctcaaaa
     2401 ttggcttttt tgagcgttgc cttggcctct gcaactgtct gacctgccac atctggaatg
     2461 gcaatggttg caggagttct ggatagtatc caaataagag aagctgccac caatacaagg
     2521 ctggccaaca aaatcaggta acgcatctta aatctatgtt ttttcggtgc ttgtggttgg
     2581 taagtttcct ctgtcacagc ctggcttggg tttttgattg atttgtgttc tgtttgcgct
     2641 tgaaccttag gaatagatgt caaggtactc tgagaaacct tcggcaaggt cttggtatct
     2701 gccttgctcg tttcatcaaa gattaactta ctttcatttc tacgattgta ggacaagcta
     2761 ctagacaagt ccacatacat ctctgaaacc gagcggtagc gattggtcaa ctttttagca
     2821 gttgccttga taataacatt ttctaaagcc tgaggtacag atggattttc tgcaataacg
     2881 gacggcaggg gtttctggaa atgctggagg gcaatggtca ccgcgctatc cccgtcataa
     2941 gggatatggc ctgtcagcat ctcatagaaa ataatcccca tggcatagat atcactctgc
     3001 acagtcgcct tcgaaccacg cgcctgctct ggtgacaagt aatgaactga gcccaacatc
     3061 gagttagtct gggtcagact tgtctctgca aaggctacag caatcccaaa gtctgtgacc
     3121 ttggcagtcc catctggtgt caagaggata ttttgaggtt tcaagtccct gtgaacaatt
     3181 cctcgagtat gggccaagcg catagccaag agaatttgtc ccatgatacg gactgcttct
     3241 tcattagaaa gaggataatg ttccttgata tagcgtttga ggtccagtcc agccacatac
     3301 tccatagcta ggtactgttg accgtcttcc tcgccaatat ctgttatccg aacgatatga
     3361 ggatggtcta gatctgccat agctctcgct tcacgctgaa aacgagctac agctatcggg
     3421 tccgtctggt agttggtcct cagaaccttc actgccactt cttccccatc taagattaag
     3481 tctttggcta ggtagacatc cgccatacct cctcgaccaa tctgtttgac aatccgatag
     3541 cgtccggcaa aaatcttgcc gatttggatc attctgcatc ctcctcgttc atagaaacaa
     3601 gggcaaccgt aatgttgtct aaacctcctg cattgttagc aaaacgaaca agtgtctccg
     3661 ttttatctgc taaaggaata tcactggtta caatatcacg aatctcactg cctgaaatca
     3721 tgttggtcaa gccgtcacta ttgagcaaga gatagtcacc tgactcaagg ataactgtcc
     3781 caaaatcagg ctgaatttca tctttttgcc caatagactg ggtgataata tttttttgcg
     3841 gatgagcttc tgcctcttct ggtgtcaatt gaccagcctt gagcaattca ttaaccaagg
     3901 aatgatcgct cgtcaactga tggtattctt ctccacgaat caagccgata cgcgaatcac
     3961 caatatgagc atagatagcc tgattatcaa taatagcaag gacttccaaa gtagttccca
     4021 tgcctctgta agcttcatcc tgaccaagct ggtgaatctt ttgattttca atttctaggt
     4081 aatgggcgaa ccattcacgc acttcattga ctgtatcgat ctgggtatca acccaagcta
     4141 cacccaggtc tgtgaccgcc atttcactag cgatattccc tgcgcgatga cctcccatcc
     4201 catcagctaa aataatcatg gtacgtccag ctctattgac atagtggttg acatagtctt
     4261 ggttatttgt tcgtttctga ccaacatctg ttaataatga aatttccatg tgtcagttcc
     4321 ttcctaatcc gatatcttgc gaaattgact gatgaagaat ccatcacttc catacaattc
     4381 aggtgtaatg aggatacagc cgtctttcat gatatcctta cattcatgtt ctagttttac
     4441 ctgctcgaac tcgggatgac tctctaaaaa ggccttaacg acttgaaaat tctcctctga
     4501 gacgatagtg caggtgctat aagttattat accacctttg cctagtattt gacaaacact
     4561 acctaatatt tctaactgaa tttcctgcaa ggacgcgaaa tctgccgttt ctttattgta
     4621 tttgatatct ggttttcggc gcaaaagacc gattcctgaa caaggagcat ccaccaaaat
     4681 cttatcaaag gaatcctggt caaaaaactc atgcaccttt ctggcatcca atttttgagt
     4741 ttgaacccga tcttcaactc ccagacgttg ggcattttct tgaattaaat ccaacttgtg
     4801 gtcgtacaag tccagagcag taacctgacc tgtcgtaaga taagaggcta tatgggctgt
     4861 tttcccacct ggagccgcac aggcatcaag cactcgctca tcaccttgta aatcaagcgt
     4921 cggagcaacc agctgactgg actcatcttg gatggtaatg gctccatccg caaacaaatt
     4981 atgccctgca aaatgcccct gctccttaac cagaccagtg gttgctaaaa gggaattatt
     5041 cgcctccaac aaggcttgga tttcctcttt tcgacttagg tctgttacac gaatactggc
     5101 tttgtttcgc actaacaggc tttcaaagat ggcttttgct ctctcctctc cgtattcttc
     5161 cttgagtttg gcaactagcc aaactgggag agaataggca atggagtcac gcttgttttt
     5221 tcgcttgatg ctagcaatat ctggccagcc ttcacgcaag atacggcgaa gaacagcgtt
     5281 gaccaatttt tcactgcctt ttttacggag tttggccaat tccactgctt cattaaccac
     5341 agcatgatct ggaatcttgt ccaaatagcg gagttggtag gcactcatga gaagaaggac
     5401 atagagccag ctgtctaact ggtctctgtc ttcgataaag tgggataggt accattccag
     5461 agtcagttta cgggctaccg ttccatagac cagctcggtc actaagccct tgtctgctgc
     5521 caaaagttga cttcccttta gatgcttatt taaggcgata tttgaatatg cttggttcac
     5581 aaaaacatcc tctagcactg ctagagctaa acttctagcc gtttctactt tagtcaccaa
     5641 atcgttctcc tacagtcaat gtacgtccaa ctccgttgag gaaggaagca atgtccatct
     5701 taggcttacc agctggctgc acttgtttga gggatagagc cccttcagcc gttgcgacaa
     5761 tcaattcttt cttgccgata gagagaatct cacctggatt tccctgacct tctactggta
     5821 gggcttcata aatcttaaag cggtcgccct taaggaaagt atgggcaaca ggccaggggt
     5881 tcattccacg aatttggtta aagagttgac gattggtttt gttccagtcc agtttttctt
     5941 cctctggctt tatatttgga gagaaggtaa cctgactcgt atcctgcggt tcaggtttga
     6001 tatcaccagc aatataggca ggcagagtgt ccaaaagcaa atcacgacca actagcgcca
     6061 atttttcaaa caaggtgcca acattgtcct catctgtgat cggaatgctg cgacgagaaa
     6121 tcatatctcc tgcatccatt tccttaacca tttccatgat ggtcacacca gcttcctcat
     6181 ccccttgaat caaggcataa tggataggcg caccaccacg gtgtctagga aggagggagg
     6241 catgaacgtt gacagcaaag tccatgctat caaggagttt gcttgggaga aactgcccaa
     6301 aagcagcagt cacaattcca tctgctccta gcttcataag atcttccatc tctggacttc
     6361 cagataattt ttcaggttgg tagatagata gtcctgcttc cttggcagcc tgcttgactg
     6421 gggtttcttg gataactttt ttacgaccaa cagcacggtc tggctgggtc acaacggcta
     6481 gaatttcgta acggtcatct gtcaaaagtc cttttaagac tgttgctgaa aagtcggggg
     6541 tccccataaa gattagtttt gtcatatctt ctccttctta taaaaattgc tgcggctcat
     6601 ggtcaatgct gagacggagc tcactatttt cccgttcttg agtcaaggct aaaacctggt
     6661 tgagggtcga ccccagctca tcttctaaac ggtatttaat taaaatctgg taatgataga
     6721 ggttgtgggt acgggcaatc ggttttggcg ttggccccag aatgggactg gtctctgaca
     6781 agcctgaccg caaaatgttc atgacttcat aggcacgttt gaaaacctct tcttctttct
     6841 tgtgagaaag ggtaatacca atcgtgaaat agtaaggtgg atagccgagt tgtcgtctga
     6901 ttcccatttc ataggcataa aagccttcgt aatcttgatc cttggcaaat cgaatagcat
     6961 agtgctgcgg attgtaggac tgtatcaaga cttgacctgc cttttcagcc cgacctgatc
     7021 gacctgccac ctgagtcaag agctggaagg ttctctcaga agaacggaaa tcaggcagat
     7081 tcaaggccgt atccgcattt agaactccga ctagggtaac attgggaaaa tccaaaccct
     7141 ttgcaatcat ctgagtacca agtaaaatat ccgcttcccc tcgcccaaac tggtcaagca
     7201 aggcttggtg actgcctttc tttcgagtcg tatccacatc catcctcaga atgcgagctt
     7261 ggggaaagag ttctgctagc tcatcataag ccttctgagt tcccgtccca tagtaacgaa
     7321 tactgcggct cttacagtta ggacagacct gaggaatatc cttcgagaaa ccacaataat
     7381 ggcagttcat agtcttggta tccatatgca aggtcagaga aatatcgcag ttgggacaag
     7441 tatccaccgt cccacactcc cgacacatga caaagctaga ataaccacgg cgattgagca
     7501 tgagaaccac ctgctctttt ttaaccagac ggtcttggat agcctctagc aaaggaggcg
     7561 taaagtttga cgtctcattt tgtccgatat agtctcgaaa gtcaatcact tgaacctcag
     7621 ggattgtagc caaaggattg gcacgttggg ttagacgtaa gtgttgatag acgcctttgc
     7681 cagcacgtgc ccggctctct aagctcggcg ttgcagatcc aagtaccaga gttgcttgat
     7741 tatactgagc ccgtaaaata gctacctctc tggcatggta acggggattg ctgtcctgct
     7801 tataagccgc ttcatgctct tcatcaataa tcatgacacc cagatttttc agaggagcaa
     7861 agatagcaga tctggcacca acaacaactt gggcatcgcc acgctccacc ttgcgccatt
     7921 catcatactt ttcaccattg gataatcctg agtgaagaat ggctaccttg tccccaaaac
     7981 gtgctataaa acgctcggtc atctgaggag tcaaggaaat ctcaggtacc agcaaaatag
     8041 ctgtcttgcc cttatccagg gcaccttgga taatctgcaa gtaaacctcg gtcttcccac
     8101 ttcctgtaat cccttgaagt agaaagggag gttgagaact gccaatagaa ctcacaaccg
     8161 catcacgcgc ctgtctttgt tctggattta actccaaagg tctacttgct tcaattcctt
     8221 caaaataagc agccgagcgt tgaacttcct tttggactat ggtaacagca ccttgatcca
     8281 caaagaagtt gacttgctct cgcgagtagg actctaacaa gctagccaag gaagcgctct
     8341 ctggatgaga cagcagataa tctctcagtt ccaacttttt cttggcacgt gtagaaatct
     8401 caacaccttc taattgagca tggtcaacct cataccaaga ctgggtcttg accttctttt
     8461 gatcgactgc ctgatattcc agaccaagca ggccttttct agtcaaacgc atcatttcag
     8521 cttgcttggc aaggtctagt gaagaaaagg ctagcgaatc ttctgaacca aacaggcgca
     8581 ctcgttcttc ctgactcaag ccttccagag gatagagaat cttgtcatag ctagaattca
     8641 gaaaccctgg aagcatggcc ttgaggatag agattttgta ggagaagaca gatttgcgta
     8701 actcctcagc cagccagagt tgttctggcg tgagaacagg agaaaaatcc agcacctctg
     8761 caatatcttt taaatcttgc tccatctctt ctccatctga ttgggacttc aaaccaagaa
     8821 caatcccttg aatcaggcga ttacccttac caaaaggcac atgaacccgc atcccaactt
     8881 ccagcattcc ctcaaattcc tccggaatcc tgtaactata gggctggtcc gtctgcatca
     8941 agggcacatc tacgataatc ttagctaggg ccatcttctc acctcctcct tgtcagtaca
     9001 ttcttgcaat agaaaaaata agattgagtc cccccaacct taaatttttt caccatcttc
     9061 tttttcttta gcaatttgct ctttgatttt cttttcttct tcttctttgc ggcgtttttc
     9121 ttcttcgata cggcgacgca ctgcttcacg ttttccttct ggatctgggt gaattgtaac
     9181 gtttcctgat tcgatttctt ctaaagcgcg aagagttgat ttttcagact tgaaaccttg
     9241 agttgctggg gcacctgctt ccaattcgtg ggcacgtttt gcttccaaga ttacgagtga
     9301 atattttgaa ggaaccttgt cgagcaaggt atcaatagag ggttttaaca tcatttgctt
     9361 gtacctattt tctaaatttt atcgggtagt tggagatttt ggtaacatct cctgatagtg
     9421 accaatgaca cgatccacac agaagtgttc tgcttcaatc acacatttga cacgttcagc
     9481 agctaggggt acctgatcgt tgacaatcgc ataatcatac tcacgcatga gggcaatttc
     9541 ttccttggcc ttttcgattc gttgggcaat cacttctgca ctatctgttc cacgacctac
     9601 caagcgatct tgcaattcat ccaaatctgg tggtgtcagg aagataaaga cagcatctgg
     9661 aacctttttc ttgacctgaa gagcaccctg aacttcaatt tcaaggaaaa catcgattcc
     9721 cttgtccaag gtttcattga cataggtcag aggagttcca tagtagttac cgacatattc
     9781 tgcgtattcc aacatctgtc cttgacgaat cagctcttca aattcttcac gagtacggaa
     9841 gaaatagtca acaccgtcca cttctccagg acgttgtgcg cgtgtcgtca tcgatacaga
     9901 atattgaaat tggttttcag aactctcaaa aatctctctt ctaaccgttc cttttccaac
     9961 ccctgaagga ccagaaaaaa cgattagtaa gcctcggtct gccattgtgt ctccttttag
    10021 tcaatctgtg aaataacatt tctctagaat aatggcaaaa agccagatta tcctttacag
    10081 tctttctatc tagtgtaaca aaaaagcagt aatttttcaa ctgctctttc ttatttattt
    10141 agcataatct actgcacgaa gctcgcgaat cacggttacc ttgatatttc ctggataatc
    10201 gagattgttt tcaattttct tacgaacttt gtgagccaag attgtgactt tgtcgtcctt
    10261 gatttttcct ggattgacca tgatacgaat ttcacgtcct gcttgaaggg caaagctagt
    10321 ttgcactcct tcaaagccgt tagcaatttc ttccaaatca tggagacgct tgatgtagct
    10381 ttcaagagac tcactacgag cacctggacg ggctgcgctc aaggcatctg ctgcagcgac
    10441 gataactgct atcacgctct cagcttcaac atctccgtgg tgactagcaa tcgtattcac
    10501 cacaactggg ggttccttgt acttacgggc caattccata ccgatttcaa cgtggctacc
    10561 ttcaacctca tggtcaatgg ctttcccgat atcgtgaagg aatccagcac gacgggcaag
    10621 agccgcattt tcaccaagtt cgctcgccat gataccagcc aacttagcaa cctcaatcga
    10681 atggcgcaaa acattttgtc catatgaagt acggaactgc aaacgtccca taatcttcat
    10741 caagtctgga tgaaggtttg gcgcaccaat ttcataggca gcagcctcac cgtattcacg
    10801 aatcttattg tcaatctctt gacggttttt ctcaaccaac tcttcgatac gagctggatg
    10861 tatacgacca tctttgagca acatttccat agtcatacgg gcaatctcac gacgaatcgg
    10921 atcaaatcct gacaaggtca ccacttctgg tgtatcgtcg ataatcacat cgacccctgt
    10981 caaactttca aaggtacgaa tgttacgacc ttcacgacca ataatgcgtc ccttcatagt
    11041 atcgtctggc agatgaactg ttgagtttgt tgactccgct acatattcac cagcgatacg
    11101 ttgcatagct tgaaccaaga tgtccttggc cattttgtca gaacgttcct tgacctcttg
    11161 ctcagcttcg cgaatgcgac tggcaatctc cctggtcaag ttttcctctg tctgagccaa
    11221 gataatatct cgtgcttctg cctgagacag cgcaccaata cgctctagtt ctgcttcttt
    11281 ttgtctttcg acttcctcta attgctcttc acgcgcatca aggtttttcg ctctatcaga
    11341 aatactttgt tctttttgtt caagtgtttg ttctttactc gtcaaattgt cgtccttacg
    11401 gtcaaggcta gtagctctct ctgtcaaacg actttcgatt tgtttgagtt cttgacgttc
    11461 tgatttgaat tcagcgtcca cttcttcacg gtattttctg gcttcttctt tggcctccaa
    11521 tagtgcttct tttttaagag acttgctttc acgtttggct tcattaacaa gtaaatccgc
    11581 ttcacgctca gcttgtccac gtaaattagt tgcttcttgt tcagcattta aaagcatcaa
    11641 ctctgcagct tcctgagatg atttcatctt agctgagatg ctgacatatc caatgactaa
    11701 accaatgatg acggcaaaaa cagcaatcgc aagcgacatg atttccatgt ttttacctca
    11761 ttttattgtt attccgaatg acatacattc ttttacattc taccataaaa aagtgatttt
    11821 cacaaaccta aaatagaata tgttttgagg aatttggaac acatttacca aaataaactt
    11881 gttgtttaga aatagtagtt tagtagagac ttgagaaaaa gcctaccttt caatagactt
    11941 agtaatgatc tttaaaggac aagaaagcca cgctatctcc atccatcata taaatcaagc
    12001 gattttctgc atcaatacgc cgtgaccagg ctccttggta atcatatttg agtggttctg
    12061 gtttacctat tcctgtaaag ggatcacgtt gaatatcctt gattagttta ttgattcttt
    12121 ttaacgtttt cttatcctga ttttgccagt agcaataatc tgcccaggca tcttctgtaa
    12181 acttgagcag catttcttac tcctcaataa catggacctg agtacttcca gcacgaactt
    12241 gagccattcc tcgcaaaacc ttatcagaaa gttccttatt ttgagcaatt ctcagggttt
    12301 cttggatact atcccactca ctctttgaaa ggactacaat gtcctcatct ggatttttat
    12361 tgaccaccgt caaaggctca aattcatcat ttaccttctt catgtagtcc tttaaatgat
    12421 ttcggaatgt tgagtaaagg actgcttcca taaccatacc tcgttttagc tcttttccac
    12481 tattatacac gaaaagaaag aaattgtcag gaacttgtac aagattttct tttctatcta
    12541 tttatactca atgaaaatca aagagcaaac taggaaacta gccgcaggct gtacttgagt
    12601 acggcaaggc gacgttgacg cgatttgaat ttgattttcg aagagtatta ttcgtaaaaa
    12661 atctcaaaaa gcctaccttt cggtagactt agtttgtttc tattctaatc ggcactcttc
    12721 caaaattttg ctctgctata cttggcttcc ctagttggta aatctggtct gctttttggg
    12781 tcatagcatc ccaaggttct ttgccaattc ggctgactag actgacctga cctttcaaag
    12841 acttgagatg ttgcctacct ttttcagtaa atccaaggac atgaatggct tctggcaagt
    12901 cactttctct agcctgcatc aaaatatagg tcaataggcg tctgacacgc gccttggtgt
    12961 aacgtttggt agtcactaac tcgaccaatt cttccacaga ctgggctgtt ttaatagctt
    13021 ctttaatgcg cacagccatt tcttgattga cctgatagat ggtggttagg tcgggatttg
    13081 acaagatttg atagcggagc aagggaaaat agtcttccca gatcacctta ctggcctgct
    13141 caaatagggc aacagaaggc ataaaacgtt ctaagaaatc ttggtccttc tgatgttgac
    13201 ggagggctgt cgccgaggca aagtccacat ctttattcac agaatggtaa cctgccccct
    13261 gacgctgaat cggatgcagc ttgatattcc gtcctgcaac cgccttggca taggccagag
    13321 caagaacatg attgggggta ttacctgaaa aatcaagacc agcaaattcc ttccacatag
    13381 cttgggtttt ctggggatag gaaagggaat caggcagatt ttccacaaat ttctccatct
    13441 cagcaccttt ctctgtgtat aagtcagcaa ttttctgata atctcgaact tcctctgtcc
    13501 caaaaactag actatcaatg cccaagcgat ccaagatatc catagctcct tgaccaaaga
    13561 aatcagctgc ctgaacactg actaaaaagg gcaattccac taccaagtct gcgccatttt
    13621 ccagcgccat ctgggtcctt gtccacttat ccacaatagc aggctctcca cgctgcatga
    13681 aattccctga catggcaaca attttcagtc cctctgcctg atccagcagg tatttatggc
    13741 cattatgaaa aggattgaac tccgcgataa tacctgttat ggtcatgcta atctcctact
    13801 tctgcgccac aaaaaaccaa cgggtgctag tttctgttgg ctccttatcc tcaaagtctg
    13861 catagagttt gaaggactta aatccagcct gttccagcaa gatatcatag gtcaagatct
    13921 cataggtcct ctcctcatgt acttcatcgt ggcgactaaa ggaaccgtca gcctccttga
    13981 taaagaaagt cagctcatgc acgatggagt gaggaacttc gtcctcatat gtatcccaaa
    14041 gcatggcaaa atcttccgca ttttcatggt aggaatagcc agggaatact tcatctgtct
    14101 ggtaggtcga gtgcacgtca aagatgaaaa ctccctcttc attcagcgca ttgtacacgt
    14161 ccttaaagac gtcccctact tccacctcat cctgcatata gcagatagag tccgaataac
    14221 acgtgacaaa atcgtatttc cctgccttgg acaaattcag catattacct tctataaaag
    14281 caatcttttg cttggctgaa gttgctctct tctccgcaat cttcaacata tccgcactca
    14341 agtcaagtcc agtcacatca aaaccagcct gagagaagcg cactgattga attcctgtcc
    14401 cacaagccaa ttccaagagt ttctttctct ccttggtctt aggcaaatga cgcagagaaa
    14461 aattcgtcca tttgtcgtat aaactatcgt ccatgaccgc atcatagacc gccgcaaagg
    14521 tttcataagt cgccataatc atgatactaa gagctccatg gtaaacacca ctagcccttt
    14581 acctttctat caatttttct aaaataagca aagaaaccca gttgactaca actgagttca
    14641 tcttctaagc aagagcttct gaaatatcta ctgaattcgc ctcatgccat agcttctcta
    14701 gattataatg ggcacgcatt tcttctgaaa agatatgcac gacaacagca ccgaggtcca
    14761 gtaagaccca gcctccagct gcatcgcctt cgacatggct acctttaaag cctgcttgag
    14821 ctactttttc acggatatta gctgcgatag cgtccaactg acggctattc attgagctag
    14881 tgatgaccaa gtagtccgtc acactagtca aatcttgtac atcaagtgcg aggatatcct
    14941 ccgcacgttt ctcatcagcc gctttcacga ctagttctag taattctttt tcgttcattt
    15001 aatcctcttt ttagaaaata tgtttgtaat ctagaacatc tgcaaagcgc tcctcccaaa
    15061 tgtcctcata aaattcatta atcgtttctg ctggaatgtc atccccatca aaggttgtga
    15121 cagcaccttc tggaacaata agctgatagc catattcaaa ggcaaccttg acagaggtat
    15181 ccacacaata ttctgtctgc ataccacata aaactaattt ttcaatcccc tgcttatcca
    15241 agtattcttt taagccagtt tctttgaaaa tactgttata cttcttctga aagacctttt
    15301 cagcaggttt tcgatttaaa agcgcagata actgccaatc ttctgatgtt tgagcttcag
    15361 agttctcaat atgttgaaca tagataattt caatattctt gcttctagcc tggttttgta
    15421 aataagaaat tttttccaat agactttttg tctggaaccc agtttctact aaaacattct
    15481 gaacatcaat gattataaaa gccgtcttca tttagtcctc tttcaaatag tgcacaaagg
    15541 cgttataggt ttcaagggtt tggggataga tggggaatcc ctgatgagct agatactcca
    15601 cggtacgagc tgtttcgtag gccaccgcct tattgagcga tagacttgca atctcacgcg
    15661 ccacatctac tccaggaaag gctcgattgt gctcgatata gtctgcgacg tagatgacct
    15721 tatctaggtc tgtcatttga ccagctccga ctgtatgaat ttcaatggct cgcagaattt
    15781 cagaatcatg caaatccaaa tcttcctgaa ttttgtaaat tccgaccata ccatgccaga
    15841 cattattgcc ccagtttttg aggtcagggt ctagctggta acggtcaatc aagactagaa
    15901 attcctgatc tgacaacttt ttagcatagt catgaagaag acctgctaga cttgctttct
    15961 caacatctac accaaagcgc tgagctaatt ccatagcggc acgctccaca cctagacaat
    16021 gggttagacg tttctcaggt agaagctctg ccattttttc caacaaggcc tcacgggagc
    16081 agttgatata gtcttgatag gacatcagta gagcccttcc ttctcgatgt agtctagcac
    16141 tggctgaggt aggagaaagt tgggtttccg accttgggca aggaagtccc gcaccatgct
    16201 ggacgagata tccatgagcg gtacgtccac ccagataact ggataggaag tccctacctt
    16261 gtagcgtgga cgctgaaccc ccacaaactg aaccatgtca accagttcat caattcggta
    16321 ccacttaggc agatagtcaa ccatgtcggc accgatgata aagtaataat ccgtatctgg
    16381 attcttctct gtcaaaatct tcatggtgtc gtaggtgtag gaaataccct tgcgctccaa
    16441 ctcaatggtt tcaatgacta ggccgtcaat tccctcaatt gccaactcaa gcatcttgag
    16501 acgatggtgt tcagggatgg tttccttttt atcaacgtga ggaggttggt attcaggcat
    16561 gagaagaact tgatccagtc ccaactgttg ccgtacttga tccgcaacaa tgagatgggc
    16621 attgtgaaca gggttaaaat tcccccctaa aatcccaact tgtttgcgtt ttttctcctt
    16681 gatttctggc tccaactcta ccttggtaaa gggagtcaat agttcgattg tcataggcta
    16741 gtccccttta tttctctata aaaacagtta tttggagttt cggtcttaga tttctttgac
    16801 tttcttagaa atcttgcgat tttctttctt gctagattgt ttaaacaaaa tcaagatgcg
    16861 tcctattttt tggactgtat ccacaccgat ttcttcttcc aaaatttcag ctacttcgtg
    16921 gatgttttca tctgtgtttt gtaagagagt aaccttgatt aattcacgcg catcaagagc
    16981 ttgacggacg ctggttttga tttggtcgtt gagtccattt ttcccgattt ggatgatagg
    17041 tttgagggtg tgtgcctggc tgttgaggaa ggcacgttgt tttgatgtta atgacataat
    17101 tttcttcctt gttttttgat agttatttta ggtggtgagg gacggtcgga actaattttc
    17161 caaagatttc atctttgaaa aatggatttc ctgacctcac aattgagtct tggtctcaat
    17221 tgtgcccctt gccaatctac agattggcaa tcacaccaca gcaataacta tcgctatatg
    17281 agctcacttt gttcgctcag cgattttgtt ttaaataatt gcttttcgtg tgacgacggc
    17341 gacgccttct ggtgcccaga cggcgacttt tgctatgcct gttacacgaa tccatcctag
    17401 gcctgagata actaggtctg tcttatcttt aatggtaaag acatgctgga ctagccttgg
    17461 gaaatcttct ttttccttgc tatttggtgg tgtcagaaga gttcccaggt gcttatcgta
    17521 gaaagcacta gctccttcaa gcttgctacg atggagtttg agttcattat caaagaaagc
    17581 agtaaatcct tgcttttctc ctgctataaa gtcaaagcgt cccaaaccac ctaaaaatag
    17641 ggtttgctca ggattaagct gataggtctt aggcttgatt tcctttttag gactgacata
    17701 cttgaggttt ttggccgtca agtagtgagc catctggtgg cggtggataa ttcccggcgt
    17761 atcgtaaata taagatccgt cgtcaagcgg aatctctatt ttgtccaagg ttgtccctgg
    17821 gaagcgtgaa gtagtgatga cattctgatc acccgtgatt tcttggataa tagcattgat
    17881 tagagttgat tttccaacgt tggtcacacc gaccacatag acatcgcggc ccttacggta
    17941 gtgttcaatc ttgtcaatga cttccttaat ggcatgttta ttttgtgctg aagttaggac
    18001 cacatcgact ggacgaagac cttcttcatg ggcacgtttc atgagccact ggctaatctt
    18061 accagactta actgacttag gaaggatatc ttttttattt cctaccaaga ggacatcatt
    18121 gcccgagacg aaacgtggta aacctgggat gacagatcca ttaaaatcaa agatatcaat
    18181 aacattgacc actaaagcat cactgtctcc cacctcgtgc aagagcttga ggaaatcatc
    18241 gttcgtcaac tggacatctg tgatttcatt gtagtggcgg agacggaaac agcgttggca
    18301 atagacttcg ccagtctcca aacctttttc aagtgccgac tggggggtaa aaccaagacc
    18361 agctttatct gtcgtctgaa tggttgctcc acaaccaata cagagaattt cttccatagt
    18421 taaattcctt ttttatatgt aatcggtccg tacttttcag tgatttttct cataacacga
    18481 cgctcacgag ttcggttaat ctgcgttttg attgagtcat gttggaccaa gggtttgact
    18541 aaaattgacc gaatccctgc acggtgggct gctcgtatat ctgtcatgag ttggtcacca
    18601 accatgacca cttccttttt gtcatagtgg aattccttca tagcacggtc aataccaaat
    18661 gtgaagggct tcaaggccca gtaaacgtaa tcaatcccaa atttctcaac tgctcgttga
    18721 acgcgttttt tggtgttatt tgacactacg ataatgccaa tacccgcgtc ccgaaggtca
    18781 tgtagccatt gcttcatctc tggcgttcca tcagggttgt tccaagcaat gagggtatta
    18841 tccaaatcga ccaaaacagc ctttattccc tgcgcctgca ggcttgggac tgtcagatca
    18901 tagactgctt ccacagcaaa atctggtata taattttcaa tcgccattct ggctcctttt
    18961 cttaactttt tttcgcaatt ttccttagta acaaggataa aacaatccac aaacctgcac
    19021 tagccaaact gatatagagc caagcatggg gctcatctgt taaaggtaag ggaacattca
    19081 ttccgaaaaa gcctgtcacg actgccaaaa cagctagcaa gactgaaatg atagtcaagg
    19141 ttgtcaaatt gtcattcaga ttattgttta gaatattgtt gtaagaggct gaaagctgct
    19201 gtaaaatctg agagattagg tctgtcatgg ataccagctg atgagcctca atcatggcat
    19261 catcaaactg ttctctctca atctcatcaa aactacgata caaggcatga ccttgaatat
    19321 gctctaacaa aatccgattt tgtttggcag ctgccgtcag ataaaccata ccagtctcca
    19381 aatcagacag gacaaagagg tttttcttgg tagttcgctg gcgcaagagg tcattgacct
    19441 catccctact cttgtccatc tgctcaatga caggatagta ggcattgctg atgatttcca
    19501 gactggcaaa gagaaactta taaatcgaaa gcgtgtcatg gttctccaga taacgagtca
    19561 tctgttcaat gacataggcg ttcttggtat tactaatggt aatcaggcga cgatgctcga
    19621 caataaaggt catgggaaaa gtctcatagt aggccttgtc cttttttacg tctaagacat
    19681 tatagataaa ggtaaccgtc tcactttcac ggtggtagtc catgtgggcg cgctcgtttc
    19741 tatccagtgc gtattcaatg gtttccttgt ccaaaccgta aatttcagaa aggtcttcta
    19801 gtttattcaa ctttcctagg tctaggtcta tccaggtaca accattgccc aactgctttt
    19861 ctaaaaccat cttttctcct tattaaactc cctctattgt accacaaatt acttaaaata
    19921 acaggtctag tctgtgcgtg agaaattgct aacaactgtg atatttcggt tagggacaaa
    19981 gtgaagggct tgctcctcgc tacgaagctg tgtcgtacga attcccacac tgacaacctt
    20041 acccgataca gtaatcggtc catttgtcag aacgacctca tctcccacat ccagttgacg
    20101 ttcaaagagg atgaaaaagc cattgatgac atcagacaga aagccttggg ctcccatacc
    20161 aatcgctacc ccagcaatac cagctccagc cagcaaacta gaaactggca aacctaaaat
    20221 cgacaaaatg cagtagagta aaaagaaata tagcgtataa ttaaacacat tttctagtaa
    20281 acgtgagatg gttttttggc gtccaacatc atgacgagac atttttagag aaggtttgac
    20341 aattctctgc accatggtat gaagcatttt tttagcaata taaaatacaa ttaaaagcag
    20401 taaaagagaa atgaccttgg ttagaatatt ctcgataatg gttgtcacat ctagcttttc
    20461 aatataagcc tgaataaatt tttgcatata aaactccttt atttcattat accatatttt
    20521 catcagtctc attctccata aatattcaaa aaatagacag aaaattcttg attttgtcta
    20581 tcatttatac tataatcata atcactacac aaaaaggaga ttattatgaa aagaatcatt
    20641 catgcttgga ataaggcaag ccttgtcaag cgtatcttga ttggtatgct ttgtggggaa
    20701 tcctaggact gacccttcct aatctctcag ggattgggct actcgaggat ttattcgttg
    20761 gaggtctaaa agctgttgct cctatcctag tctttgctct cgttgccaat gccctttccc
    20821 aacatcaaaa gggacaagat agcaatatga aaactgttgt tttcttgtat atcctatgat
    20881 agggactttc gccgctgctc ttgtagctgt actagcaaat ttcatcgtcc ctattgaaat
    20941 taccccaaat agtgccaata ctgaaattgc accaccagat gggattgggc aggttctcag
    21001 caacctcttg ctcaaactgg ttgacaaccc agtcaacgcc ctgcttactg ctaactatat
    21061 tagaatctta tcttgggcag tcatttttgg aatcgctatg agagaagcca gtaaaaatag
    21121 tcaagaattg ctaaaaacta tcgctgacgt gacttctaaa attgtcgaat ggatcatcaa
    21181 tctggctcca tttggaatcc ttggtcttgt ttttaaaacc atttctgaca agggagtcgg
    21241 aagccttgcc aactacggta ttttattggt tctattagta acgactatgc tttttgttgc
    21301 ccctgtggtc aaccctttga ttgccttctt ctttatgaga cgcaatcctt accctctagt
    21361 ttggaactgc ctccgtgtca gcggtgtgac agcctttttc actcgtagtt ctgcgactaa
    21421 cattcctgtc aacatgaaac tctgccatga ccttggactc aacccagata cctattctgt
    21481 ttctatccca ctcggttcta ctatcaatat ggctggagta gcgattacca ttaacctttt
    21541 gacccttgct gcagttaaca ctcttggaat tcctgttgac tttgccacag cctttgtcct
    21601 cagtgtggta gcagctatct catcctgtga tgcttcaggt attgccggag gttccctcct
    21661 tcttatccca gttgcttgta gccttttcgg tatttctaac gatattgcca tacaaattgt
    21721 tggggttggt tttgtgattg gtgtcatcca agactcatgt gaaacagccc ttaactcttc
    21781 tacagatgtc ctctttaccg ccgttgccga atacgcagca acccgtaaaa aataactcat
    21841 caaggcaagc ctgctcatgt cttgtctttt acgcttttat tctaacttat taggaaattc
    21901 ttatgtctat tagccaacgt acgaccaagc tcatcttagc tacctgtctt gcctgcctgc
    21961 ttgcttattt tctcaatctt tcgtcagcag tttcggctgg aattatcgct ctcttgagcc
    22021 tatctgatac gcgtagaagt actttaaaac tggctcgcaa tcgtcttttt tctatgcttc
    22081 tagctctggc tatcggtgtt ctagcttttc acttgagcgg atttcatatc tggagtctcg
    22141 gcctctatct ggccttctac gttcctttag cctacaagat gggctgggaa attggcatca
    22201 caccaagcac tgttttggtt agccatctct tggttcaaga gtcaacctct ccagaccttc
    22261 tagtcaatga attccttctc tttgctattg gtacaggatt tgccttgctt gttaatctct
    22321 atatgccttc acgagaagag gaaatccagc actaccacac gctggtggaa gaaaagttaa
    22381 aagatatcct ccagcgcttc aaatactatt tatccagagg agacggacgc aaccgagcac
    22441 agctggtagc agaattagac acgcttttga aagaagccct cagactggtc tatttggatc
    22501 actctgacca cctctttcac cagacagact accatatcca ctactttgag atgagacagc
    22561 gacaaagtcg tatcctgaga aacatggccc aacagattaa cacttgtcac cttgccgcca
    22621 gtgaaagcct gatcttagcg caactctttt caaaaattgc aggtcaactg agccagacca
    22681 atcctgcttc tgatttgcta gatgaaattg aacgttatct ggaagtcttc cggaaccgca
    22741 gtctgcccaa gacaagagaa gaatttgaaa cccgcgccac ccttcttcaa ctcctacgtg
    22801 aagccaaaac cttcatccaa gtaaaagttg atttttacca aaaatataga cagtaatact
    22861 cttcaaacca cgtcagcgtc gccttgctgt atatatgtta ctgacttcgt c
//
LOCUS       DAVHEX010000035        22636 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVHEX010000035 DAVHEX010000000
VERSION     DAVHEX010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22636
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.035"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..366
                     /locus_tag="VVL45_001302"
     CDS             <1..366
                     /locus_tag="VVL45_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4187471.1"
                     /translation="AMDRFFYTVLLSIVVLLFWDSLAILLTMFINDYRRRDIVIRFVL
                     TPLGFTAPVFYLIDSAPSIVRWIGQLNPLTYQLTILRNFYFKNSTTLELVFLLLTSLL
                     VLISVSFIIPKIKLILIER"
     gene            524..997
                     /gene="tnpA"
                     /locus_tag="VVL45_001303"
     CDS             524..997
                     /gene="tnpA"
                     /locus_tag="VVL45_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISSpn6 family
                     transposase"
                     /protein_id="HEU4187472.1"
                     /translation="MREDNQSLSHTTWNCKYHIVFAPKYRRQIIYGRYKASIGRIIRD
                     LCERKGVIIHEANACSDHIHMLISIPPKLSVSSFMGYLKGKSSLMIFDKHANLKYKYG
                     NRKFWCRGYYVDTVGRNQKVIAEYIQNQLQEDRVADQLTLFESVDPFTGEINKRK"
     gene            1254..1475
                     /locus_tag="VVL45_001304"
     CDS             1254..1475
                     /locus_tag="VVL45_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU4187473.1"
                     /translation="MYRRLRDLREDHDLPQKQIATILSFTNSAYAKIERGEHALTADV
                     LVKLSDFYDVSTDYLLGLTDFPDKIRFRK"
     gene            1922..2224
                     /locus_tag="VVL45_001305"
                     /pseudo
     CDS             1922..2224
                     /locus_tag="VVL45_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            2380..3609
                     /locus_tag="VVL45_001306"
     CDS             2380..3609
                     /locus_tag="VVL45_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU4187474.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGITIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            3629..6325
                     /gene="ppc"
                     /locus_tag="VVL45_001307"
     CDS             3629..6325
                     /gene="ppc"
                     /locus_tag="VVL45_001307"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEU4187475.1"
                     /translation="MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYETIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    6408..6625
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6738..7742
                     /locus_tag="VVL45_001308"
     CDS             6738..7742
                     /locus_tag="VVL45_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU4187476.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNVTGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            7811..8677
                     /locus_tag="VVL45_001309"
     CDS             7811..8677
                     /locus_tag="VVL45_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4187477.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQNVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            8674..9432
                     /locus_tag="VVL45_001310"
     CDS             8674..9432
                     /locus_tag="VVL45_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4187478.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEDLTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            9522..11282
                     /gene="dnaG"
                     /locus_tag="VVL45_001311"
     CDS             9522..11282
                     /gene="dnaG"
                     /locus_tag="VVL45_001311"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEU4187479.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVPFIEAVQILGQRVGIEVEKPLYSEQ
                     KSASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKRSSGKASEIYLMEGFMDVIAAYRAGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAATLKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFLEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMEGISRPTPITMPVT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            11285..12394
                     /gene="rpoD"
                     /locus_tag="VVL45_001312"
     CDS             11285..12394
                     /gene="rpoD"
                     /locus_tag="VVL45_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEU4187480.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            12408..12737
                     /locus_tag="VVL45_001313"
     CDS             12408..12737
                     /locus_tag="VVL45_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU4187481.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            12852..13895
                     /gene="cpoA"
                     /locus_tag="VVL45_001314"
     CDS             12852..13895
                     /gene="cpoA"
                     /locus_tag="VVL45_001314"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HEU4187482.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAVLKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            13911..15236
                     /locus_tag="VVL45_001315"
     CDS             13911..15236
                     /locus_tag="VVL45_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU4187483.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLQQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            15267..15443
                     /locus_tag="VVL45_001316"
     CDS             15267..15443
                     /locus_tag="VVL45_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU4187484.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            15502..15636
                     /locus_tag="VVL45_001317"
     CDS             15502..15636
                     /locus_tag="VVL45_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEU4187485.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            15700..17010
                     /gene="obgE"
                     /locus_tag="VVL45_001318"
     CDS             15700..17010
                     /gene="obgE"
                     /locus_tag="VVL45_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEU4187486.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEDFKKKLAENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            17020..17178
                     /locus_tag="VVL45_001319"
     CDS             17020..17178
                     /locus_tag="VVL45_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187487.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            18321..19580
                     /locus_tag="VVL45_001320"
     CDS             18321..19580
                     /locus_tag="VVL45_001320"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU4187488.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            19596..20147
                     /locus_tag="VVL45_001321"
     CDS             19596..20147
                     /locus_tag="VVL45_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4187489.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLFIITHLENKASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            20140..21417
                     /locus_tag="VVL45_001322"
     CDS             20140..21417
                     /locus_tag="VVL45_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU4187490.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            21433..22293
                     /locus_tag="VVL45_001323"
     CDS             21433..22293
                     /locus_tag="VVL45_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEU4187491.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
BASE COUNT         6926 a         3883 c         5046 g         6781 t
ORIGIN      
        1 gcaatggata gattttttta tacagttttg ttatctattg ttgttttatt attttgggac
       61 tcccttgcaa ttttacttac aatgtttatc aatgattaca gaagacgtga tattgtaata
      121 cgttttgtac taacaccgct tggttttaca gctcctgttt tctacttaat agattctgct
      181 cctagtattg tgagatggat tggtcagtta aatcccttaa cttatcaatt aactattttg
      241 agaaactttt attttaaaaa ttcaacaact ttggaattag ttttcttatt gttaacatca
      301 ttacttgtcc ttatatctgt atcttttatt ataccaaaga taaaattgat actgatagaa
      361 agataaaagt tgggtcatcc aacttttttg ttgtctcccg aaaaccacta gctatgctag
      421 tggttccata gagcttttag cgtggtaaca aaaagaacct cctaaaatga taagatagaa
      481 gtggtttctc cgccactaca acatatcata caggaggtac ctcatgagag aggataatca
      541 aagtttatca cataccacat ggaattgtaa atatcatatt gtttttgcac ccaaatatcg
      601 tcgtcaaatc atttatggca gatacaaagc tagtatcgga agaatcatac gtgacttatg
      661 tgagcgtaag ggtgtaataa tccatgaagc gaatgcttgt tcagaccata ttcacatgct
      721 tatcagtatt cctccgaaac ttagtgtttc gtcctttatg ggctatttaa agggcaagag
      781 cagtttgatg atttttgata agcatgcgaa tttaaaatac aaatatggca atcgcaagtt
      841 ttggtgtaga ggctattatg tagatacggt aggccgtaat cagaaagtga tagctgaata
      901 tattcagaat caattacaag aagacagagt agcagaccag ctcacgttat tcgagtcagt
      961 agatccgttt actggcgaaa taaataagag gaagtaacta aggtgcttta gcacctgctc
     1021 gggaaagtgg tgcgcgagga agctatttcg gtgggccttt ggccctggcc ggtagaagcg
     1081 gcttatagcc gcagaacaaa ccaccagttc acactggtgg ttttgattta aaaaacttga
     1141 tacataaaaa taaaagtcta tataaaggat ggtaaaattc ctgttgtccg atttggacaa
     1201 tatcctaaat agttacaata tatggtctat actttttctt aggagaaagc tagatgtaca
     1261 gacgtttgag agatttgagg gaggatcatg atctgcccca aaagcaaata gctacaatac
     1321 tttcgtttac aaattcagct tatgccaaaa ttgaacgggg tgagcatgcg ttgacggctg
     1381 atgtattggt taaactctca gatttctatg acgtcagtac agactattta ttgggattaa
     1441 ctgattttcc tgataaaatt cgctttagaa aataatctcc tcaatttcat agagtttgaa
     1501 aatgagtgag attttttatt tgccctttga caactgaata gcctaaaatg gtactttcct
     1561 catttgtgga gcaaatttga atggctcgcc atgataagag cgattttaaa atcatcaata
     1621 aaatagagcg atactttata tgccatgata caaatgatat acaatgatac ttctgaccgt
     1681 tcagcctgcc aacgtaaaag agcagcaagt gaaattctta tgatgacttc atcagtcatg
     1741 ccacgttgaa tgtgtgagtt tgttagataa acgcaattaa tcctcaaaag gttccccgaa
     1801 ccttttgagt tctacagacg catcacgtgg agtgtgtaag cttgttgcta aaagcgtaaa
     1861 aaccttggaa cgaaaggaat aatagacttt ctgcgaaaca aaaatataat acaataaaac
     1921 tatgaatgat gaagcaagta aacaattgag cgatagccgt ttcaagatcc ttgtaggtgt
     1981 tcagcgcacg acttttgaag agatgttagc tgtgttaaaa acagcttatc aacgtaaacg
     2041 cgcaaaaggt ggacgaaaaa gcaaattaag cctagacgat ctccttatgg taactattca
     2101 atacatgcga gaatagagca cttatgaaca aattgcggct gattttggca ttcacgaaag
     2161 caacttaatc cgtcggagtc aatgggttga agcaactctt attcaaaatg gttttacgat
     2221 ttcaaattct gccttaattc tgtaaaaaca gtaaaattcg aaggattgta aggtaagagt
     2281 ttttttcttt ctgaaaaaat ggtataatag caatcaaaac tagaaaataa aacggaattt
     2341 ggaacagatt tgtctgtatc ctagtagagt ggtgatacta tgaagattag taagaggcac
     2401 ttattaaatt attccatctt gattccctac ttgcttttat ctattttggg cttgattgtg
     2461 gtctattcga ccaccagtgc tattttaatt gaagaaggca agagcgcctt gcagttggtt
     2521 cgaaaccaag gaatcttttg gattgttagt ttgatactga ttgccttaat ttataaattg
     2581 agactagatt ttttgagaaa tgagcgacta atcattttag ttatattaat agaaatgctt
     2641 ttattgttct tggctcgttt tattggtatt tccgtaaacg gggcatacgg ttggatttcg
     2701 gttgcaggaa taactattca gccagctgag tacttaaaaa tcattattat ttggtattta
     2761 gctcaccgat tctccaaaca gcaagaagaa atagctactt atgattttca agttttgact
     2821 caaaatcaat ggcttccccg tgcttttaat gattggcgat tcgttctcct agttctgatt
     2881 ggaagtttgg gaattttccc tgatttagga aatgcgacta ttttagtctt ggtttccttg
     2941 attatgtata cagttagtgg aatcgcttat cgctggtttt caaccattct ggcgctcgta
     3001 tctgccgctt ctgtctttgt cttgaccact atcagcctaa tcggtgttga gaccttttca
     3061 aaaattccag tattcggcta tgtagccaag cgctttagtg ccttttttaa tccttttgcc
     3121 gatcgtgctg atgcaggtca ccagttagct aattcttatt ttgccatggt caatggcggt
     3181 tggtttggtc taggtcttgg aaactcgatt gaaaaacgag gttatttgcc agaagctcat
     3241 acagactttg tcttttctat cgtgattgaa gaatttggct ttgttggtgc cagtcttatt
     3301 ttagctctct tgtttttcat gattttgcgg attatcttgg tcggtatccg agcggagaat
     3361 cctttcaatg ccatggttgc actcggtgtc ggagggatga tgttggttca ggtatttgtc
     3421 aatatcggag ggatttcggg cttgattcca tctacaggag tgactttccc cttcttatcc
     3481 cagggtggaa atagtcttct agtcttatca gtggcagtag cctttgtctt aaatattgat
     3541 gccagtgaaa aacgcgctaa attgtaccga gaattggaaa atcaaccaat gaaccttctg
     3601 ttgaagtagg ataaagaaag gatagtttat gtctcttcaa aaattagaaa attatagtaa
     3661 taaaagtgtt gtgcaagaag aagtcttgat tctaacagaa ttactggaag atattactaa
     3721 aaatatgctt gccccagaga cctttgaaaa aataatacag ttgaaagaat tatcaacgca
     3781 ggaagattat caaggtctaa accgtctagt gactagctta tcaaatgatg aaatggtcta
     3841 tatttcacgc tatttctcta tcttgcctct tttgattaat atttcagagg atgtggattt
     3901 agcttatgaa atcaatcatc aaaataatat tgatcaggac tatttaggta aattatctac
     3961 aacgattaaa ttggtagcag aaaaggaaaa tgccgttgag atcctagaac acttgaatgt
     4021 tgtccctgtt ttgacagccc atccaacaca agtgcaacgc aaaagtatgt tggatttaac
     4081 aaatcatatt catagtcttt tgcgtaaata ccgtgatgtt aagttggggt tgatcaataa
     4141 agataaatgg tacaatgatt tgcgtcgtta catcgaaatt atcatgcaga cagacatgat
     4201 tcgtgagaaa aaattaaaag tgactaacga aatcacgaat gctatggaat attataacag
     4261 ctcctttttg aaagctgtac ctcatttgac gacggagtat aagcgcttag cgcaagcgca
     4321 tggtctgaat ttaaaacagg ctaaaccaat caccatgggt atgtggatag gtggtgaccg
     4381 tgatggaaat ccatttgtta cagcaaagac cttgaagcag tctgcactca ctcagtgtga
     4441 agtcatcatg aactactatg ataaaaagat ttaccaactt tatcgtgaat tttctctttc
     4501 aactagcatt gtcaacgtca gcaagcaagt cagagaaatg gctcgtcaat ccaaggataa
     4561 ctcgatttac cgcgaaaaag agctttaccg tcgtgccttg tttgatattc aatcaaaaat
     4621 tcaggcaact aaaacctatc tgattgagga tgaagaagtt gggactcgtt atgaaaccgc
     4681 caatgatttc tacaaggatt tgattgccat tcgagattct ctactagaaa ataagggcga
     4741 gtccttgatt tcaggtgatt ttgtggaatt attgcaggca gtagagatat ttggttttta
     4801 cttagcatca attgatatgc gacaagactc tagcgtctat gaagcctgtg tggcagaact
     4861 cttgaaatca gcaggaattc attctcgtta tagcgagttg agcgaagaag aaaagtgtga
     4921 ccttctcttg aaagaattag aagaagatcc ccgaattctt tctgcgactc acgcagaaaa
     4981 atcagaatta ttagcaaaag aattagctat ttttaagacg gctcgtgttt tgaaagataa
     5041 gttgggagat gatgtcatcc gtcagaccat catttcacat gcaaccagcc tttctgatat
     5101 gctagaatta gctattctgt taaaagaagt aggactggtg gatacggaaa gggcgcgtgt
     5161 tcagattgtt cccctttttg aaacaattga agacttggat cattcagagg aaacaatgag
     5221 aaaatatctt tctcttagcc ttgccaaaaa atggattgac tcacgaaata actaccaaga
     5281 aatcatgctt ggctactctg acagtaataa agatggcggt tacttgtcat catgttggac
     5341 cctctacaag gctcaacaac aattgactgc tattggagat gaatttggcg ttaaggttac
     5401 cttcttccat ggtcgtggtg gtactgtcgg tcgtggtggt gggccaacct atgaagccat
     5461 tacatctcaa ccgctcaagt ctatcaagga tcgtatccgc ttgacggagc agggtgaagt
     5521 aattgggaat aaatacggta acaaagacgc cgcttactat aaccttgaaa tgctagtatc
     5581 ggcagctatt aaccgtatga ttactcagaa gaagagcgat accaataccc caaatcgtta
     5641 tgaaaccatt atggatcaag tagtggaccg tagttacgat atctaccgtg atttggtctt
     5701 tggtaatgag catttctatg attatttctt cgagtcaagt ccaatcaagg ctatttcaag
     5761 ttttaatatt ggttctcgtc cagccgctcg taagactatt actgaaatcg gtggtttgcg
     5821 tgccatccct tgggtattct catggtcaca gagtcgtgtt atgttccctg gatggtacgg
     5881 ggttggttca agcttcaagg aatttatcaa taaaaatcca gagaatattg ctatcttacg
     5941 agatatgtac caaaattggc ctttcttcca atcgcttctt tcaaatgttg atatggtttt
     6001 gtcaaaatca aatatgaata ttgcttttga atatgctaaa ctttgtgaag acgagcaagt
     6061 taaggccatc tatgagacta ttttaaatga atggcaagtt actaagaacg ttatcttggc
     6121 tattgaagga catgacgaac tcttagctga caatccatat ctaaaagcta gtctggatta
     6181 ccgtatgcct tactttaata ttctcaacta tattcagttg gagttgatta aacgccaacg
     6241 tcgtggagaa ttgtccagtg atcaagaacg attgattcat atcaccatca acggaattgc
     6301 gacaggattg cgtaattcag gttgataatt ttcaagagtg aatgctaaaa gtgaatatca
     6361 aaaaaattct aatagactat tgacaagtag tttaaaaatg atataattta accattcaga
     6421 aaagtaatca tacaaacttt ttagagagtc tgtggtagct gaaaacagat aagtggcaat
     6481 gatgaaaatt gggctgaatg ctatttagaa tttgaaatta taaaaattcg gtaagcacac
     6541 cttacagtgc atctcgttat tgcgagactg agcgataggg aaattcccta taattgaggt
     6601 ggtaccgcgc atcgacgtcc tcacacaagt tttttgtgtg aggatttttt tgatggaggt
     6661 tagtatggaa agaaaacgat ggcgtcgctt gtttagataa gtgaaatatg ttaaaggaaa
     6721 taaaaaggag aaacagaatg aaaaataaac gtttaattgg aattattgct gcattagcag
     6781 tcttagtagc aggaagcttg atttattctt caatgaataa atcagaagct cagaataata
     6841 aggatgagaa gaaaataacc aagattggtg tgcttcaatt tgtgagccat ccatcccttg
     6901 atttgattta taaagggatc caagatggac ttgcagaaga aggatataaa gatgatcaag
     6961 ttaaaattga ttttatgaac tcagaaggtg accaaagtaa ggttgcgaca atgagtaaac
     7021 aattggttgc aaatgggaat gaccttgtgg ttggtatcgc aacaccagca gcccaagggt
     7081 tggctagtgc aacaaaagac ctaccggtta tcatggccgc tattacagac ccaattggtg
     7141 ctaacttggt taaagatttg aaaaaaccag gtggcaacgt tacaggggta tctgaccaca
     7201 atccagctca acaacaagtt gaactcatca aggctctgac accgaatgtg aaaacaatcg
     7261 gagctcttta ctcaagtagc gaagacaatt caaaaacaca ggtcgaagaa tttaaggctt
     7321 atgctgaaaa agcaggtctg acagtggaaa catttgcagt tccttcaaca aatgaaattg
     7381 cctcaactgt cactgttatg actagcaagg tagatgctat ttgggttcca attgataaca
     7441 ccattgcatc aggatttcca acggttgtct ctagcaatca aagttctaag aaaccaattt
     7501 atcccagtgc gacagctatg gtagaagtag gtggtttggc atcagttgta attgaccaac
     7561 atgaccttgg tgtggcaaca ggtaaaatga ttgtgcaagt cttgaaaggt gcaaaaccag
     7621 ccgatacccc agtcaatgtc ttttcaactg gtaagtcagt catcaataaa aaaatagcac
     7681 aagaactagg tattactatt cctgagtctg ttctcaaaga agcaggacaa gtcatcgaat
     7741 aaataaggta gggaggagga gactcttccc attttttaaa aagatgaata tagaaaggat
     7801 taaataaaat atgattgttt ccattatttc tcaaggattt gtctgggcta ttctaggtct
     7861 gggaatcttt atgacattta ggattttaaa ctttccagat atgacgacag aaggttcctt
     7921 ccctcttggg ggagctgttg ctgtcacttt gataaccaaa ggcgtgaacc catttttagc
     7981 gacacttgtt gctgtaggag caggttgttt ggctggaatg gcagcaggcc ttctttatac
     8041 aaaagggaag atcccaacct tgctctcagg gattttggtg atgacttctt gtcactcaat
     8101 catgctcttg attatgggac gtgcgaattt aggcctgctt ggaaccaagc aaattcagaa
     8161 tgttttgcct tttgattcgg atttgaatca actcttgaca ggtctcatct ttgtgagtat
     8221 tgttattgct ctcatgctct ttttcttgga cactaaactc ggacaagcct atattgctac
     8281 aggggataat cctgatatgg ctagaagttt cgggattcat actggacgca tggagctcat
     8341 gggcttggtc ttatcaaatg gtgtgattgc ccttgcaggt gccctcattg ctcagcaaga
     8401 aggttatgcc gatgtgtctc gagggatcgg ggttatcgtt gtggggcttg caagtttgat
     8461 tattggagaa gttattttca agagtttgag cttggcagag cgtttggtta ctatcgttgt
     8521 aggttctatc gcttatcaat ttttagtgtg ggcagttatc gcacttggct ttaatacaag
     8581 ttaccttcgt ttatacagtg ccttgatttt agcagtctgc ctcatgattc caacatttaa
     8641 gcaaacaatc ttgaaaggag ccaagttaag caaatgacag caattgtaga attaaaaaat
     8701 gcaaccaaaa tcgttaaaaa tggctttgat gaagaaaaga ttattttaaa tgatgtttcc
     8761 ttagaaattt ttgaacggga ctttatcacg attttgggcg gaaatggtgc tggaaaatca
     8821 actctcttta acactatagc agggacctta tcactaacta gtggaactat ccgtatttta
     8881 ggtgaagatc tcactaagtt ttcacccgag aagcgtgcca agtacctgtc tcgtgtcttc
     8941 caagatccaa agatggggac agctccccgt atgacggtcg ctgaaaatct tttaatcgcc
     9001 aagtttcgtg gtgaaaagcg tggattgtta ccacgacgct tgactagcta taaggatgaa
     9061 tttcaggcaa ccattgaaaa agtaggaaat ggtcttgaga aacacttgaa tacaccgatt
     9121 gagttcttat caggtggaca aagacaggct ttgagtctct tgatggcaac cttgaagcga
     9181 cctgaattac tcctgttaga tgagcatact gctgccctgg atccaaagac tagtgttgct
     9241 ttgatggaat tgacagatga atttgttaag aaagatcagc taacagccct tatgattact
     9301 catcatatgg aagatgctct caaatacggc aatcgcttga ttgtcatgaa agaaggacga
     9361 attatccaag atttaaacca agaagaaaaa gcaaaaatga aaatctctga ttattatcaa
     9421 ctctttgaat aaagtaggga aaatgagtga aatggtttac tttttttctg aaataaagta
     9481 tactatataa agtaaactat gataacatgg aggtattgtg tatggttgac aaacaagtca
     9541 ttgaagaaat caaaaacaat gccaacattg tggaagtcat aggagatgtg atttctttac
     9601 aaaaggcagg acggaactat ctagggctct gtccttttca tggtgaaaaa acaccatctt
     9661 tcaatgttgt agaggacaag cagttttatc actgtttcgg ttgtggtcgc tcaggtgatg
     9721 tctttaagtt catcgaggag taccaagggg ttccctttat agaagcagtc caaatcttag
     9781 gtcagcgtgt tggaattgag gttgaaaaac cgctttatag tgagcagaag tcagcctcac
     9841 ctcaccaagc tctttatgat atgcacgaag atgcagccaa attttaccat gctattctca
     9901 tgacaacgac tatgggcgaa gaggccagaa attaccttta tcagcggggg ttgacagatg
     9961 aagtgcttaa acatttttgg attggtttag cacctccaga acgaaactat ctctatcaac
    10021 gtttgtctga tcagtatcgt gaagaggatt tactggattc aggcctgttt tatctctcgg
    10081 atgccaatca atttgtagac acctttcaca atcgcattat gtttcccctg acaaatgacc
    10141 aaggaaaggt cattgccttc tcaggtcgta tctggcaaaa aacggattca caaacttcta
    10201 agtataaaaa cagccgatcg actgcaattt ttaacaaaag ttacgaatta tatcatatgg
    10261 atagggcaaa aagatcttct ggaaaagcta gtgagattta cctgatggaa ggattcatgg
    10321 atgttattgc agcctatcgg gctggaatcg aaaatgctgt ggcgtcgatg ggaacagcct
    10381 tgagtcgaga gcatgttgag catctgaaaa ggttaaccaa gaaattggtt cttgtttacg
    10441 atggagataa ggctgggcaa gccgcgacat tgaaagcatt ggatgaaatt ggtgatatgc
    10501 ctgtgcaaat cgtcagcatg cctgataact tggatcctga tgagtatcta caaaaaaatg
    10561 gtccagaaga cttggcctat ctattaacga aaactcgtat tagtccgatt gagttctaca
    10621 ttcatcagta caaacctgaa aacagtgaaa atctgcaggc tcagattgag tttcttgaaa
    10681 aaatagctcc cttgattgtt caagaaaagt ccatcgctgc tcaaaacagc tatattcata
    10741 ttttagctga cagtctggcg tcctttgatt atacccagat tgagcagatt gttaatgaga
    10801 gtcgtcaggt gcaaaggcag aatcgcatgg aaggaatttc cagaccgacg ccaatcacca
    10861 tgcctgtcac caagcagtta tcggctatta tgagggcaga agcccatcta ctctatcgga
    10921 tgatggaatc ccctcttgtt ttgaacgatt accgtttgcg agaagacttt gcatttgcta
    10981 cacctgaatt tcaggtctta tatgacttgc ttggccagta tggaaatctt cctccagaag
    11041 ttttagcaga gcagacagag gaagttgaaa gagcttggta ccaagtttta gctcaggatt
    11101 tgcctgctga gatatcgccg caggaactta gtgaagtaga gatgactcga aacaaggctc
    11161 tcttgaatca ggacaatatg agaatcaaaa agaaggtgca ggaagctagc catgtaggag
    11221 atacagatac agccctagaa gaattggaac gtttaatttc ccaaaagaga agaatggagt
    11281 aataatggca acaaaacaaa aagaagtaac aacatttgac gtacaggtag cagaatttat
    11341 ccgtaatcat aagcaaaaag ggacagcaac agatgatgaa atcaatgcaa gtctggttat
    11401 tccttttacc ttggacgctg atgggattga agatctcttg caacggattc aggatgcagg
    11461 catttctatc acagataacg aaggaaatcc aagtgcgcgt gttctcagca atgaagaaga
    11521 accagaactc agcgatgagg acttgattgg gtcaacttct gctaaggtca atgaccctgt
    11581 ccgtatgtac ttgaaagaaa taggggtcgt tcctctcttg accaatgaag aggagaaaga
    11641 gttggcactg gctgttgaag ctggtgatat cgaagccaaa caacgtcttg cggaagccaa
    11701 tcttcgtttg gttgtttcca ttgccaaacg ctatgtcggt cgtggtatgc agttccttga
    11761 cttgattcaa gaaggaaata tgggcttgat gaaggcggtt gacaagtttg actattctaa
    11821 agggttcaag ttttcaactt atgcaacttg gtggattcgt caggctatca ctcgtgctat
    11881 tgcggaccaa gctcgtacca tccgtatccc agttcacatg gttgaaacta tcaataaatt
    11941 ggttcgtgaa cagcggaatc tccttcaaga attggggcaa gatccgacac cagaacagat
    12001 tgctgaacga atggatatga cacctgataa ggttcgtgaa atcttgaaga ttgcccaaga
    12061 accagtatct cttgaaactc ctatcggtga agaggacgat agccaccttg gagactttat
    12121 cgaagatgaa gtgattgaaa atccagtgga ttatacgact cgtatcgtct tgcgtgagca
    12181 attggatgaa atcttagata ctcttacaga ccgtgaagaa aatgttctgc gtctacgttt
    12241 tggactagat gatggaaaaa tgcgcacact tgaagatgtg gggaaagtct ttaacgtaac
    12301 tcgtgagcgt atccgtcaga ttgaagcaaa ggctttgaga aaactacgcc aaccaagtcg
    12361 tagcaaaccg cttcgtgatt ttattgaaga ctaagagtga ggaaaatatg gcttatacag
    12421 aagagcaaat tgaaaacatc aaaacacgga ttttaacagc cttggaagaa gtcatcgacc
    12481 ctgagttggg aatcgatatt gtcaatcttg gtttgatcta tgagattcgt tttgacggtg
    12541 acacagggca aacagagatt gacatgactt tgacaactat gggttgtccc ttagcagacc
    12601 ttttgacaga ccagatttat gatgctatga tagaggttcc agaagtaacg gatactgagg
    12661 ttaaattagt ctggtatcca gcctggacag ttgaaaaaat gagtcgctat gcccgcattg
    12721 ccctaggcat taagtaaaca tataaaagca tgtgagagac tgttggaaaa gcgaggaaat
    12781 ttcccctctt ttcctctagt ctctcctttc ttttgctgat tttattcaaa gaaaatgata
    12841 taatagtagt tatggagaaa aagaaattac gcatcaatat gttgagttca agtgagaaag
    12901 tagcaggaca gggagtttca ggtgcttacc gtgaattagt tcgtcttctt caccgtgctg
    12961 ccaaggacca attgattgtt acagaaaatc ttccaatcga ggcagatgtg actcactttc
    13021 atacgattga ttttccctat tatttatcaa ccttccaaaa gaaacgctca gggagaaaga
    13081 ttggctatgt gcatttcttg ccagctacac ttgagggaag tttgaaaatt ccatttttct
    13141 taaagggaat tgtgaaacgc tatgtatttt ctttttacaa ccggatggag cacttggttg
    13201 tggtcaatcc tatgtttatt gaggatttgg tagcagctgg tattccacgt gaaaaagtga
    13261 cctatattcc taactttgtc aacaaggaaa aatggcatcc tctaccacaa gaagaggtag
    13321 tcagactgcg cacagatctt ggtcttagtg acaatcagtt tatcgtagta ggtgctgggc
    13381 aagttcagaa acgtaaaggg attgatgact ttatccgtct ggctgaggaa ttgcctcaga
    13441 ttacctttat ctgggctggt ggcttctctt ttggtggtat gacagatggt tatgaacact
    13501 ataagaaaat tatggaaaat ccccctaaaa atttgatttt tccaggcatt gtatcgccag
    13561 agcggatgcg cgaattgtat gctctagcgg atcttttctt gttgcctagt tacaatgagc
    13621 tctttcctat gactatttta gaagctgcga gttgtgaggc tcctattatg ttgcgtgatt
    13681 tagatctcta taaggtgatt ttggagggaa attatcgggc gacagcgggt agagaagaga
    13741 tgaaagaggc tattttggaa tatcaagcaa atcctgctgt cttaaaagat ctcaaagaaa
    13801 aggctaagaa tatttccaga gagtattctg aagagcatct gttacaaatc tggttggact
    13861 tttatgagaa acaagccgct ttagggagaa agtaaaaagt gaggtaatct atgcgaattg
    13921 gtttatttac agatacctat tttcctcagg tttctggtgt tgcgaccagt attcgaacct
    13981 tgaaaacaga acttgaaaag cagggacatg ctgtttttat ctttacgacg acagataagg
    14041 atgtcaatcg ctacgaagat tggcaaatta tccgcattcc aagtgttcct ttctttgctt
    14101 ttaaggatcg tcgctttgcc taccgaggtt ttagcaaggc acttgaaatt gctaaacagt
    14161 atcagctaga tattatccat actcagacag aattttctct tggcctgttg gggatttgga
    14221 ttgcgcgtga attgaaaatt ccagtcatcc atacctatca cacccagtat gaagactatg
    14281 tccattatat tgctaagggg atgttgatcc ggccgagtat ggtcaagtat ctggttagag
    14341 gtttcctgca tgatgtggat ggggttattt gccctagtga gattgtccgt gacttgctat
    14401 ctgattataa ggtcaaggtt gaaaaacggg tcattcctac tgggattgaa ttagccaagt
    14461 ttgagcgtcc ggaaatcaag caggaaaatt tgaaagaact gcgtagtaaa ctagggattc
    14521 aagatggtga aaagacgttg cttagtcttt cgagaatctc ctatgaaaaa aatattcaag
    14581 cagttttagc agcctttgct gatgttctga aagaggaaga caaggttaaa ctggtagtag
    14641 ctggggatgg cccttatctg aatgacctca aagagcaagc ccagaaccta gagattcaag
    14701 actcagtcat ctttacaggg atgattgctc ctagtgagac ggctctttac tataaagcgg
    14761 cggatttctt catttcggca tcgacaagcg aaacgcaagg tttgacctac ttggaaagct
    14821 tagccagtgg aacacctgtc attgctcacg gaaatcctta tttgaacaac ctcatcagtg
    14881 ataaaatgtt tggaaccttg tactatggag aacatgattt ggctggtgct attttggaag
    14941 ccctgattgc aacaccagac atgaacgagc ataccttatc agagaaattg tatgagattt
    15001 cagctgagaa ctttgggaaa cgagtgcatg agttttatct ggatgccatt atttcaaata
    15061 acttccagaa agatttggct aaagatgata cggtcagtca gcgtatcttt aagacagttt
    15121 tgtatcttca gcaacaggtg gttgctgtac ctgtaaaagg atctagacgc atgttgaagg
    15181 cttcaaaaac acagttgatc agtatgagag actattggaa agaccatgaa gaatagaaag
    15241 aggaacagct atgaaaaaaa caattaatga gaagcggtcg tgataaaaag attgcgggtg
    15301 tttgtgctgg ggtggcccat tatctggata tggatccgac tatcgttcaa gtcatttggg
    15361 gtgttcttac ttgctgttac ggagctggaa ttgtagctta cattatttta tggattatcg
    15421 caccagtagc aactgactat tgaaaactag ctcaattcat gctaagataa tagaaaatag
    15481 aatgtaacga aggagagaaa aatggcattt ggagataatg gaaatcgtaa aaaaactatg
    15541 tttgagaaaa taaccttgtt tatcgtgatt atcatgctag tagcaagttt attgggaatt
    15601 tttgcaactg caattggtgc cctcagtaat ctataaaata gattcaagaa aatttagtga
    15661 ctgggatttc ccagcccttt tttaaagtga gaagaaataa tgagtatgtt tttagataca
    15721 gctaagatta aggtcaaggc tggtaatggt ggcgatggta tggttgcctt tcgtcgtgaa
    15781 aaatatgtcc ctaatggagg cccttggggt ggtgatggtg gtcgtggagg caatgtggtc
    15841 ttcgttgtag acgaaggact acgtaccttg atggatttcc gctacaatcg tcatttcaag
    15901 gctgattctg gtgaaaaagg gatgaccaaa gggatgcatg gtcgtggtgc tgaggacctt
    15961 agagttcgag taccacaagg tacgactgtt cgtgatgcgg agactggcaa ggttttaaca
    16021 gatttgattg aacatgggca agaatttatc gttgcccacg gtggtcgtgg tggacgtgga
    16081 aatattcgtt tcgcgacacc aaaaaatcct gcaccggaaa tctctgaaaa tggagaacca
    16141 ggtcaggaac gtgagttaca attggaacta aaaatcttgg cagatgtcgg tttagtagga
    16201 ttcccatctg tagggaagtc aacactttta agtgttatta cctcagctaa gcctaaaatt
    16261 ggtgcctacc actttaccac tattgtacca aatttaggta tggttcgcac ccaatcaggt
    16321 gaatcctttg cagtagccga cttgccaggt ttgattgaag gggctagtca aggtgttggt
    16381 ttgggaactc agttcctccg tcacatcgag cgtacacgtg ttatccttca catcattgat
    16441 atgtcagcta gcgagggccg tgatccatat gaggactacc tagctatcaa taaagagctg
    16501 gagtcttaca atcttcgcct catggagcgt ccacagatta ttgtagctaa taagatggac
    16561 atgcctgaga gtcaggaaaa tcttgaagac tttaagaaaa aattggctga aaattatgat
    16621 gaatttgaag agttaccagc tatcttccca atttctggat tgaccaagca aggtctggca
    16681 acacttttag atgctacagc tgaattgtta gacaagacac cagaattttt gctctacgac
    16741 gagtccgata tggaagaaga agcttactat ggatttgacg aagaagaaaa agcctttgaa
    16801 attagtcgtg atgacgatgc gacatgggta ctttctggtg aaaaactcat gaaactcttt
    16861 aatatgacca actttgatcg tgatgaatct gtcatgaaat ttgcccgtca gcttcgtggt
    16921 atgggggttg atgaagccct tcgtgcgcgt ggagctaaag atggggattt ggtccgcatt
    16981 ggtaaatttg agtttgaatt tgtagactag gagactggta tgggagataa accgatatct
    17041 ttccgagatg cggatggtaa ttttgtttcc gccgcagacg tttggaatga aaagaaattg
    17101 gaagaactat ttaatcgtct caatccaaat cgtgccttga gattggcacg aactaaaaag
    17161 gaaaatccat ctcagtaaag aagctaaaaa atcccgtgcc tcatcagaca cgggattttg
    17221 tggtacgaca ggcatgtata gcaaactgaa tctggaatag cacagcatat cttctaaaat
    17281 atagtaaaat gaaatgagaa caggacaaat cgatcaggac agtaaaatcg atttctaaca
    17341 atgttttaga agcagagatg tactattcta gtttcaatca actatattgt tataaattga
    17401 tttgaatttc aaaattaaat tgtttgattc ttatttcaat ttgttatagt atatctgatg
    17461 tcaaagttct cggcgagtca aatagcgatt cccaagcctg actatcgtga ggtagcggat
    17521 taaaatggtc tggggataga ccgttttaag tctgacgctg gaaataagaa ttgtcagaag
    17581 aagggatagc gaaatcgtgg ctctacgaac aggaacgtga taataaggcg tatatagcgg
    17641 ataagagggc atcaaactct aaagtccaaa aaggtagtcg taacctatat gcgtaaatca
    17701 cgagagtaat tgaattcgta ctaagatttt ctattttcac tgtaaccttt taacgccctt
    17761 atatcttgta tacacgagga aagatgtacg acttatcccg tgaggtctat cactataaag
    17821 agaaaacgac agatagaagt gatcctgagt cacggttatc tgtctgatag gacggtatgt
    17881 ataaaacgct tctgtgaact gagagaaggg ggagaagttc ttgctaaaat ttagttgaac
    17941 agccgtattc cgatacttag ataagagatc tagtcttagc tcctactcag ttttagggga
    18001 taaaaaaggg gcaatagcga ttcgagaaag attatactct tcgaaaatct cttcaaatca
    18061 cgtcaatatc gccttgtcgt atgtgtagga tactgactac gtcagttcca tctacaacct
    18121 caaaacagtg ttttgagcaa cctgcggcta gtttcctagt ttgatctttg attttcattg
    18181 agtattagta attcagttac taactcgtca actctgattt atccaataaa attgaaaagg
    18241 atggaaaaaa ggataaattt atgatatact ttattttgaa gaccttatta gaaatcttga
    18301 aagagtattg aaaacttaga atgagaaaaa ttgttatcaa tggtggatta ccactgcaag
    18361 gtgaaatcac tattagtggt gctaaaaata gtgtcgttgc cttaattcca gctattatct
    18421 tggctgatga tgtggtgact ttggattgcg ttccagatat ttcggatgta gccagtcttg
    18481 tcgaaatcat ggaattgatg ggagctactg ttaagcgtta tgacgatgta ttggagattg
    18541 acccaagagg tgttcaaaat attccaatgc cttatggtaa aattaacagt cttcgtgcat
    18601 cttactattt ttatgggagc ctcttaggcc gttttggtga agcgacagtt ggtctaccgg
    18661 gaggatgtga tcttggtcct cgtccgattg acttacacct taaggcgttt gaagctatgg
    18721 gtgccactgc tagctacgag ggagataaca tgaagttatc tgctaaagat acaggacttc
    18781 atggtgcaag tatttacatg gatacggtta gtgtgggagc aacgattaat acgatgattg
    18841 ctgcggttaa agcaaatggt cgtactatta ttgaaaatgc agcccgtgaa cctgagatta
    18901 ttgatgtagc tactctcttg aataatatgg gtgcccatat ccgtggggca ggaactaata
    18961 tcatcattat tgatggtgtt gaaagattac atgggacacg tcatcaggtg attccagacc
    19021 gcattgaagc tggaacatat atatctttag ctgctgcagt tggtaaagga attcgtataa
    19081 ataatgttct ttacgaacac ctggaagggt ttattgctaa gttggaagaa atgggagtga
    19141 gaatgactgt atctgaagac agcatttttg tcgaggaaca gtctaatttg aaagcaatca
    19201 atattaagac agctccttac ccaggctttg caactgattt gcaacaaccg cttacccctc
    19261 ttttactaag agcgaatggt cgtggtacaa ttgtcgatac gatttacgaa aaacgtgtaa
    19321 atcatgtttt tgaactagca aagatggatg cggatatttc gacaacaaat ggtcatattt
    19381 tgtacacggg tggacgtgat ttacgtgggg ccagtgttaa agcgaccgac ttaagagctg
    19441 gggctgcact agtcattgct gggcttatgg ctgaaggtaa aactgaaatt accaatatcg
    19501 agtttatctt acgtggttat tctgatatta tcgaaaaatt acgtaattta ggagcggata
    19561 ttagacttgt tgaggattaa accgtagagg tgtttatgaa tatttggacc aaattagcaa
    19621 tgttttcttt ttttgaaacg gatcgcttgt atttgcgtcc tttctttttt agtgatagtc
    19681 aggacttccg cgagatagct tcaaatccag aaaatcttca atttattttc ccaacgcagg
    19741 caagtctgga agaaagtcaa tatgcactgg ccaattactt tatgaagtcc cctttgggag
    19801 tgtgggcaat ttgtgaccag aaaaatcaac aaatgattgg ttctattaaa tttgagaagt
    19861 tagatgaaat caaaaaagaa gctgagcttg gctatttttt gagaaaagat gcttggtcgc
    19921 aaggatttat gacagaggtt gttagaaaaa tttgtcagct ttcttttgag gaatttggct
    19981 taaaacaatt atttatcatt acccaccttg aaaataaagc tagccaaaga gttgctctta
    20041 agtctggatt tagtttgttc cgtcagttta agggaagtga tcgttacaca agaaaaatgc
    20101 gggattatct tgaatttcgg tatgtaaaag gagagttcaa tgagtaagca tcaggaaatt
    20161 ctaagctatt tggaggaatt accagtaggt aaaagggtca gtgttcgtag catttcgaat
    20221 catctaggag ttagtgatgg aacagcctat cgggctatta aagaagctga aaaccgtgga
    20281 attgtggaga cccgtcctag aagtggaaca attcgtgtta aatcccagaa agttgctata
    20341 gagagattaa cgtttgctga aattgcagaa gtgacttctt ctgaggttct ggctgggcaa
    20401 gaaggtttag agagagaatt tagtaagttt tcaattggtg ccatgactga acaaaatatc
    20461 ttgtcttacc ttcatgatgg ggggctcttg attgtcggag accgaacccg tattcagttg
    20521 ctagccttgg aaaatgaaaa tgcagttctg gttacagggg gatttcaggt tcatgatgat
    20581 gtgcttaaac tggccaatca aaaagggatt cctgttctaa gaagtaagca tgataccttt
    20641 accgtcgcga ccatgatcaa taaagccttg tcaaatgtcc aaatcaagac tgatattctg
    20701 acagttgaga aactttatcg ccctagtcat gagtatggtt ttctgagaga gacagataca
    20761 gttaaagatt atttggactt ggttcgtaag aatcgtagca gccgtttccc tgttatcaat
    20821 caacatcagg tcgttgttgg tgttgtaacc atgagagacg ctggtgataa atcaccaagc
    20881 acgacaattg ataaggttat gtctcgtagt ctatttttgg ttggattatc gacaaatatt
    20941 gccaatgtga gtcaacggat gatcgcagaa gactttgaaa tggtaccagt tgttcgaagc
    21001 aatcaaactt tgcttggcgt tgtgacgcga cgagatgtca tggagaagat gagccgttcc
    21061 caagtttcgg ctctaccaac tttttctgag cagattggac aaaagctctc ttatcaccat
    21121 gatgaagtag tcattacagt ggaacccttt atgctagaaa aaaatggagt tttggctaat
    21181 ggtgtattgg cagaaattct gacccacatg acccaagatt tagttgttaa tagtggtcgc
    21241 aatctcatta tcgagcagat gctgatctac tttttgcagg ctgttcagat agatgatata
    21301 ttgcgcattc aggcacggat tattcatcat acgagacggt cagctataat tgattacgat
    21361 atttatcatg gtcaccagat tgtttcaaaa gcaaatgtga ctgttaaaat taattagaaa
    21421 ctaggagaaa agatgataac attaaaatca gctcgtgaaa tcgaagctat ggacaaggct
    21481 ggtgattttc tagcaagtat tcatataggc ttacgtgatt tgattaagcc aggcgtagat
    21541 atgtgggaag ttgaagaata tgtccgccgt cgttgtaaag aagaaaattt ccttccactt
    21601 cagattgggg ttgacggtgc catgatggac tatccttatg ctacctgttg ctctcttaac
    21661 gatgaagtgg ctcacgcttt ccctcgtcat tatatcttga aagatggtga tttgctcaaa
    21721 gttgatatgg ttttgggagg tcccattgct aaatctgacc taaatgtctc aaaattaaac
    21781 ttcaacaatg ttgaacaaat gaaaaaatac actcagagct attctggtgg tttagcagac
    21841 tcatgttggg cttatgctgt tggtacaccg tccgaagaag tcaaaaactt gatggatgta
    21901 accaaagaag ctatgtacaa gggtattgag caagctgttg ttggaaatcg tatcggtgat
    21961 atcggtgcgg ctattcaaga atacgctgaa agtcgtggtt acggtgtagt gcgtgatttg
    22021 gttggtcatg gtgttggccc aactatgcac gaagaaccaa tggttcctaa ctatggtatt
    22081 gcaggtcgtg gactccgtct tcgtgaagga atggtcttaa ccattgaacc aatgatcaat
    22141 acaggcgatt gggaaattga tacagatatg aaaactggtt gggcgcataa gaccattgac
    22201 ggtggattgt catgtcagta tgaacaccaa tttgtcatta cgaaagatgg acctgttatc
    22261 ttgactagcc aaggtgaaga aggaacttat taataaaaag tgaaaagact actggaagtt
    22321 tattttgata aaaaatccag tagatctttt cataataaaa cgcattgtat caagtgttag
    22381 gggctgatat catgcgtttt tctgctttta agattttttc caactctgtt tgtaagcgca
    22441 tcataacaaa gggtctagga ttcagggctc tcctcctata tactattagt aaagtaaaac
    22501 taagggagga tattttagtg tcgcagtcta ttgttcctgt agagattcca caatattgtc
    22561 gttttgattc taaaaagaga aatggaattc tgtttaatgt tcgtattgcc aatcttaaat
    22621 ttactttttt atatta
//
LOCUS       DAVHEX010000036        22454 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVHEX010000036 DAVHEX010000000
VERSION     DAVHEX010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22454
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.036"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            67..834
                     /locus_tag="VVL45_001324"
     CDS             67..834
                     /locus_tag="VVL45_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEU4187492.1"
                     /translation="MIGISLIPDLYNIIFLSSMWDPYGQLSDLPVAVVNNDKEASYNG
                     NTMAIGKDMVSNLKENKTLDFHFVDEEEGKKGLEDGDYYMVVTLPSDLSEKTTTLSNI
                     QSTAAYQSLTSEQQTEISDSVSQNSTDSIQSAQSIVALVQDLQGSLENLQNQSSNLST
                     LKNQSNQVSPITSTSLIGLSSGLTEIQGDVTSKLVPASQSIASGVNAYTTGVDKVSQG
                     ASQLSEKNATLTGSLDKLVSGSNTLTQKSSRLTAGVG"
     gene            919..1194
                     /locus_tag="VVL45_001325"
     CDS             919..1194
                     /locus_tag="VVL45_001325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187493.1"
                     /translation="MADGSGKLAEGGTKLTSGLEDLQTGLASLGQGLGNASDQLKSVS
                     TESKNAEILSNPLNLSKTDNDQVPVNGIAIAPYMISVALFLQQYQQI"
     gene            1296..1598
                     /locus_tag="VVL45_001326"
     CDS             1296..1598
                     /locus_tag="VVL45_001326"
                     /inference="COORDINATES: protein motif:HMM:TIGR03062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HEU4187494.1"
                     /translation="MVYGGVQLIGLTANHEMRIFILIILTSLVFMSMVTTLATWNSRI
                     GAFFSLILLLLQLASSAGTYPLALTNDFFRSINPWLPMSYSVSGLRQTISINKSFS"
     gene            complement(1699..4251)
                     /locus_tag="VVL45_001327"
     CDS             complement(1699..4251)
                     /locus_tag="VVL45_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEU4187495.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQSIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSNLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAIYLTTLLKFGLIGLSTFFSLNKLFQSIPQILKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLFPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVAGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTEKIENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQK
                     KVDILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTK
                     TFTEAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQ
                     AQTGFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(4313..5935)
                     /locus_tag="VVL45_001328"
     CDS             complement(4313..5935)
                     /locus_tag="VVL45_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4187496.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTNQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
     gene            6141..7166
                     /gene="trpS"
                     /locus_tag="VVL45_001329"
     CDS             6141..7166
                     /gene="trpS"
                     /locus_tag="VVL45_001329"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEU4187497.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            7318..8796
                     /gene="guaB"
                     /locus_tag="VVL45_001330"
     CDS             7318..8796
                     /gene="guaB"
                     /locus_tag="VVL45_001330"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEU4187498.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(8847..9944)
                     /gene="recF"
                     /locus_tag="VVL45_001331"
     CDS             complement(8847..9944)
                     /gene="recF"
                     /locus_tag="VVL45_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEU4187499.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSAQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKAAVNGN"
     gene            complement(9947..10315)
                     /gene="yaaA"
                     /locus_tag="VVL45_001332"
     CDS             complement(9947..10315)
                     /gene="yaaA"
                     /locus_tag="VVL45_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEU4187500.1"
                     /translation="MEYKLFEEFITLQALFKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            10474..11724
                     /locus_tag="VVL45_001333"
     CDS             10474..11724
                     /locus_tag="VVL45_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU4187501.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPVVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYCSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKSWIAKLEQI
                     DKDAICRVANNVKLQAIYFMEGIE"
     gene            11721..13004
                     /locus_tag="VVL45_001334"
     CDS             11721..13004
                     /locus_tag="VVL45_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU4187502.1"
                     /translation="MTKVVFEEKYYPAVKEMVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTFVTEVDGDVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLDLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEITSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGEIIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            13046..13867
                     /locus_tag="VVL45_001335"
     CDS             13046..13867
                     /locus_tag="VVL45_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU4187503.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            13878..14423
                     /gene="pgsA"
                     /locus_tag="VVL45_001336"
     CDS             13878..14423
                     /gene="pgsA"
                     /locus_tag="VVL45_001336"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEU4187504.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            14420..15247
                     /locus_tag="VVL45_001337"
     CDS             14420..15247
                     /locus_tag="VVL45_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEU4187505.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            15232..16071
                     /locus_tag="VVL45_001338"
     CDS             15232..16071
                     /locus_tag="VVL45_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEU4187506.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            16064..16858
                     /locus_tag="VVL45_001339"
     CDS             16064..16858
                     /locus_tag="VVL45_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU4187507.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SISLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            16917..17735
                     /gene="mreC"
                     /locus_tag="VVL45_001340"
     CDS             16917..17735
                     /gene="mreC"
                     /locus_tag="VVL45_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEU4187508.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            17735..18229
                     /gene="mreD"
                     /locus_tag="VVL45_001341"
     CDS             17735..18229
                     /gene="mreD"
                     /locus_tag="VVL45_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEU4187509.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLIYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            18323..19501
                     /locus_tag="VVL45_001342"
     CDS             18323..19501
                     /locus_tag="VVL45_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEU4187510.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            19725..20504
                     /gene="rpsB"
                     /locus_tag="VVL45_001343"
     CDS             19725..20504
                     /gene="rpsB"
                     /locus_tag="VVL45_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEU4187511.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAALETQADSIEEIVEVVEGDNA"
     gene            20583..21623
                     /locus_tag="VVL45_001344"
     CDS             20583..21623
                     /locus_tag="VVL45_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEU4187512.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            21869..22192
                     /gene="tnpB"
                     /locus_tag="VVL45_001345"
     CDS             21869..22192
                     /gene="tnpB"
                     /locus_tag="VVL45_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEU4187513.1"
                     /translation="MIIQLSDLGQVHLVCGKTDMRQGIDSLAYVVKTHFELDPFSDQV
                     FLFCGGRKDRFKVLYWDGQGFWILYKRFENGKLTWSSTEKDVKALAPEQVDWLMKGFS
                     ITPQI"
     gene            complement(22333..>22454)
                     /locus_tag="VVL45_001346"
     CDS             complement(22333..>22454)
                     /locus_tag="VVL45_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEU4187514.1"
                     /translation="RGFKNKAFRTLEDVIQGLEKEVIKSIVNRRRTRMLFENR"
BASE COUNT         7166 a         3609 c         4597 g         7082 t
ORIGIN      
        1 aaaaaaggag aaactaagtt taaagaatgg aaagcaattt aaaaaaaacc aacctttatt
       61 attgtcatga tcgggatttc tcttattcca gatctgtaca atatcatatt tttgtcatca
      121 atgtgggatc catatgggca attgtctgac ttacctgtgg cagttgtaaa taatgataaa
      181 gaggcttcct ataatggtaa tactatggca ataggaaaag acatggtgtc caatttaaaa
      241 gaaaataaaa ccttggattt tcattttgta gatgaagagg aaggaaagaa gggattggaa
      301 gatggcgatt actatatggt agtgacttta ccaagtgatt tatctgaaaa aacaactaca
      361 ttatccaata ttcaatcgac agcagcttat caatcattga caagtgagca acaaactgag
      421 ataagtgatt ctgtatctca aaattcaact gatagtattc aatcggctca gtcaattgta
      481 gctttagtac aagatttaca gggaagttta gaaaacttac aaaatcaatc ttctaatctt
      541 tcgactttaa aaaatcaatc taatcaagta tcacctatta cttctacttc tttgatagga
      601 ttgtcaagtg gattaacaga gatacaagga gatgttacta gcaaattagt tcctgccagt
      661 cagtcgattg catcaggtgt aaacgcatat actacaggtg ttgataaagt ttctcagggc
      721 gcaagtcaac taagtgaaaa aaatgccacc ttgacaggta gtttggataa actagtttca
      781 ggctcaaaca ccttgacaca aaaatcttct agattgacag caggagttgg ttaattacaa
      841 tcaggatctg ggcaattagc agacaaatcc agtcagttac tttcaggtgc ttctccatta
      901 gagaatagag ctaataaatt ggcagatgga tctgggaaac tagcagaagg tggaacaaag
      961 ttaacttctg gattggaaga tttacagaca ggacttgctt ctttaggaca aggactaggt
     1021 aatgctagtg atcaactcaa atcagtatca acagaatcta aaaatgcaga gattttgtca
     1081 aatccactca atctttcaaa aacagacaat gatcaagttc ctgtaaatgg aatcgcaata
     1141 gctccttata tgatatcagt tgctcttttt ttgcagcaat atcaacaaat atgatatttg
     1201 cgaaattgcc ttcaggacgt catccagaga gccgttgggc ttggttgaaa tcttgagctg
     1261 aaataaatgg tattatagct gttttggcag gaattttggt atatggagga gttcagctta
     1321 ttggtttaac tgctaatcat gagatgagaa tatttattct catcatccta acaagtttag
     1381 tattcatgtc tatggtgacc actttagcaa cgtggaatag ccgtatagga gcttttttct
     1441 cacttatttt gcttttacta cagttagcat caagtgcagg tacttatcca cttgctttga
     1501 caaatgattt ctttagatct attaatccct ggttaccaat gagctattca gtttcgggat
     1561 tacgacaaac aatctctatc aacaagtcat tttcctagct gtcatactag ttctatttac
     1621 tagtttaggt atgctagcct atcaacataa gaaaatggaa gaagattaaa aaaatcgacc
     1681 gattaactgg tcgatttttt atgccttaga tgactttcgt ctgtgattat agattccaaa
     1741 tagtaagaga gaagtaaagg aacagattgc tccagtaata aaaccattgg gaatgaagga
     1801 aagtgtaata gttcctttcc ccttgggaat gtcaactttc ataaatccag tttgagcttg
     1861 tttaatttct attttcttac catcttggta ggcagaccaa cctttgtcat aaggaatggt
     1921 gaagaaaata gatgtatctt gttggacatc atatgtagca aaaaccttgt ttttagaagt
     1981 tgatactgtg acaggttgtt ctttaatttt ttgaattgcc tcggtgaaag ttttggtatc
     2041 taaacgatag aaggtaggag attcaaatga tacttgtgaa tttccaggga aactaacatt
     2101 gatattgaaa gtttttttct ctttagtata tcctagatta aagaaggaga agacattatc
     2161 agttgtaaaa gtcttttttt caccatttac aaggatgtca accttctttt gtttatcgtt
     2221 agaaaagtga aggtttatga aagagagata aacttggctg ttttctggaa cttcaatttg
     2281 atactggatt gctgcatctt catttgaaga acttgtgaca ctaatcaaat cattagtatt
     2341 ttctattttt tctgtttttt cataaggtat tggagaaaaa taatcaaaat tgacgttagc
     2401 aagttgattt aaaaatgagg cctgattatc caaggtatgt tcattgaact tgacatcatt
     2461 gtaaacagat tgactcgcaa ctgcaatcgg aagagagtat tgattttcat atagggtaag
     2521 attatctttt tgatagatat ctttaaagcc atacttatca ataggactgt ctgagatatt
     2581 gtactggata ccaaataaac tatcagccaa aatactatta tttgcatatc ggagattgag
     2641 attagtccca gaggatttaa aaccaagttt atctaaagta gagcttgatg aacgatttcg
     2701 aacagatgaa aattgagaga ttccattgta gttgaatttc atactgtcat ttcctgtctg
     2761 agtttgtagt ttttcagtac gagtaaattg atttccaata tatgttgaga aagattccat
     2821 agctgggata tctcgactat aagcacttcg agaagcaaat ccccattcct tagcaattcc
     2881 gtccatttga gatgaagcat ttaaactcat ttcaaccagt ataaataaag agattagaat
     2941 ggcaaataga ttcacagata taaacttttt gataactgca aggagtaaaa gagaatagac
     3001 aaccaaaaat tcaagagtaa gcagaatatt caaatctgtt aaaaaagaat aatgcgattt
     3061 tagatagatg gtagctaaaa atcctgctac tacaagaaaa agcgaaacta aaaaattcca
     3121 gactttaagt tctttcagac gctttaagac ttctgctgct gtgtaaatta acaaggtaga
     3181 gaaaatccaa gcatagcgat gtaaaaacat gtttggagta tgcatgcctt gccaaaataa
     3241 gtcaagagct tctatgtaaa agcttgcaat tagaaatgca aagaatatta catatatgag
     3301 tttcacgtga aacttaatag atttcagcgt aaaaaataaa atggtcaaaa taaagggaaa
     3361 tagtccaaca aaaatcattg ggatggcccc atactttgtt gtgtcaaagg aaccaatgaa
     3421 ttgcttagca aagagatcaa gataccagct actttcagtt tgaaactttg taacttcagt
     3481 caatttttcc ccatgtgtct gtaaatcaaa tagagtggga agagtcataa tcaaactagc
     3541 cataccagct aaaaaggaga taactatgaa atcaagaaca gatgattttc gagtcttaaa
     3601 gtcccacgaa atttgacaga gataccagaa aataagaaac aatactgtca tatatccaaa
     3661 ataataattt tgaataaata agattgacag acttgtaaag tacaatagga gtttcttttc
     3721 agttatcagt agatgtaaac cagttataat taaaggaatc aagataaaaa catctagcca
     3781 ggtttttatc tctaattgac tgacagtgaa actcatcaga gcataggaag tagataaggc
     3841 tagttttaaa atctgaggga tagattgaaa caatttattc aaactaaaaa aggttgacag
     3901 accaatcaat ccaaatttta agagagttgt cagatagata gcatctggca tattcgttag
     3961 atcaaaaaag taaaccagag gcgcgagaaa actacccaag taataactag atagggcata
     4021 gaagtttagc cctagaccac ttgtaaaggt gtaaaacaga ttactatttc catgtaggat
     4081 atttcgtaag gctacatcaa aaataacgta ttgatgaaag ccatctccta atagaggaga
     4141 gttgtcgcta ttccagtaga tactttgaga tagatatact ccagacataa tcactacagg
     4201 aatgatgaaa gaaataaaat aggttcgata tgtttttaaa aatgatttca tgttacctcg
     4261 tagaatgata gaaaactcag ttggttaacc caactgagtt ttgaagtttt atttagtctt
     4321 tccaaagttc tttaactttt gcttgtactt ctgcattttc taggaattca tcgtaggttt
     4381 catcgatacg gtcaatgacg ccatttttag ataagacaat gatatggtta gccaaagttt
     4441 gaataaattc gtggtcatgg ctggcaaaga tgattgattc tttaaagttt ttcaatccat
     4501 cattcaagct tgagatagat tccaagtcca agtgatttgt tggatcatca agtacaagga
     4561 catttgattt taagagcatg agttttgaaa gcatgacacg aactttttct ccccctgaca
     4621 agacatttac aggtttgtta acttcatctc cagagaagag catacggccg aggaagccac
     4681 gtaggaaagt attgtcatct tcttctttac ttgcgaattg acgcaaccag tcaagaattg
     4741 attctcctcc tgcaaaatca gctgagttat cttttggtag gtaagattga ttagttgtaa
     4801 ctccccactt gacagttcct tcatagtcaa tatctcccat gattgcacga attaatgcag
     4861 tcgtttgaat atcattttgt ccaataagtg ctgtcttatc atctggacgc aagatgaaac
     4921 taatattatc caagatagtt tcaccatcaa tctttacagt taaattttct actgtcaaga
     4981 gatcattacc aatctcacgt tccgctttaa agttgataaa tggatattta cgactagatg
     5041 gcacaatctc ttctagctca atcttatcaa gcattttctt acgtgatgtt gcctgccttg
     5101 acttagaagc attggcagag aaacgagcaa caaattcttg caattgttta attttttctt
     5161 ctgctttagc attacggtct gctagcaatt tagcagcaag ctcagaagat tccttccaga
     5221 agtcgtagtt tccgacatag agtttgattt ttccaaagtc aaggtcggcc atgtgagtac
     5281 aaactttgtt taagaagtga cggtcgtggg atactacgat aactgtgtta tcaaagtcaa
     5341 tcaagaagtc ttctaaccaa gtaatcgatt ggatatccaa accgttagta ggctcgtcca
     5401 agagaagaac atctggttta ccaaaaagtg ctttggcgag gagaaccttt actttttcac
     5461 cgttggccaa ttcgctcatg ttttggtagt gtaattcttc tggaatgttt aggttttgaa
     5521 gtagttgaga ggcttcactc tctgcttccc aacctccaag ttcggcaaac tctccttcga
     5581 gttcggcagc acgaaccccg tcctcgtctg agaaatcttc cttcatgtag atagcatctt
     5641 tctctttcat gatgctataa agtttttcat ttcccatgat aacgacatca atggcacgtt
     5701 catcttcgta gtcaaagtga ttttgacgaa gaacagagag acgttcatct ggaccaagag
     5761 agatgtgacc agtagtaggt tcgatatctc cagctaaaat ttttaaaaag gttgattttc
     5821 cggcaccatt agcaccgatt aatccgtaag tatttccttc tgtaaatttg atattgacat
     5881 catcaaaaag tttgcgatca ctaaaacgta gtgaaacatc agatactgta agcaatgttt
     5941 ttctcctata tgtgtaatat atttattcta ctagaaaata cagaaatatt caaattttta
     6001 tttgtcaatt ttgtgtaaat tatatttaca gtatccttta cacaaatctg taaaaagcaa
     6061 ggctgattta ttttgataaa ttacggttat ttcattaaaa aaatgctata attgaaagga
     6121 ctatatcgaa ggagaacaaa atgactaaac ccattatttt aacaggagac cgtccaacag
     6181 gaaaattgca tattggacat tatgttggaa gtctcaaaaa tcgagtatta ttacaggaag
     6241 aggataagta tgatatgttt gtgttcttgg ctgaccaaca agccttgaca gatcatgcca
     6301 aagatcctca aaccattgta gagtctatcg gaaatgtggc tttggattat cttgcagttg
     6361 gattggatcc aaataagtca actattttta ttcaaagcca gattccagag ttggctgagt
     6421 tgtctatgta ttatatgaat ctagtttcgt tagcacgttt ggagcgaaat ccaacagtca
     6481 agacagagat ttctcagaaa ggatttggag aaagcattcc gacaggattc ttggtctatc
     6541 caatcgctca agcagctgat atcacagctt tcaaggctaa ttatgttcct gttgggacag
     6601 atcagaaacc aatgattgag caaactcgtg aaattgttcg ttcttttaac aatgcatata
     6661 actgtgatgt cttggtagag ccggaaggta tttatccaga aaatgagaga gcagggcgtt
     6721 tgcctggttt agatggaaat gctaaaatgt ctaaatcact aaataatggt atttatttag
     6781 ctgatgatgc ggatactttg cgtaaaaaag taatgagtat gtatacagat ccagatcata
     6841 tccgcgttga ggatccaggt aagattgagg gaaatatggt tttccattat ctagatgttt
     6901 ttggtcgtcc agaagatgct caagaaattg ctgatatgaa agaacgttat caacgaggtg
     6961 gtcttggtga tgtgaagacc aagcgttatc tacttgaaat attagaacgt gaactgggtc
     7021 ctattcgtga gcgccgtatt gaatttgcta aggatatggg agaagtttat aatatgattc
     7081 aaaaaggtag tgaaagagcg cgtgaagttg cgggtcaaac cctatctgag gtaaaagggg
     7141 caatgggact tcattacttt aactaagttt attttacaag aaactatctt ttctatctcg
     7201 aagaaaagat agttttttat ttacccctgt aacatttgac aaatttttat agaatggtat
     7261 gatagataca atataaaaag agtcaagctc aaaaagaaag aaaagaggaa acttcgaatg
     7321 tctaattggg acactaaatt tttgaaaaaa ggttttacct ttgatgatgt attgcttatt
     7381 ccagctgaaa gtcatgtgtt gcctaacgat gcagatttaa caactaaatt ggcagataat
     7441 ttgactttaa atatcccaat tattaccgct gccatggaca cagttacaga gagtcaaatg
     7501 gccattgcta ttgctcgtgc aggcggtctc ggagttatcc ataaaaacat gtcaattgct
     7561 caacaagcag acgaggttcg taaggtaaaa cgttctgaaa atggagttat tattgatccg
     7621 ttcttcttga cgcctgaaca tacaattgct gaagcagatg agcttatggg tcgttaccgc
     7681 atcagtggtg ttccagttgt tgaaacactt gaaaatcgta aattggttgg tattttgaca
     7741 aaccgagatc ttcgttttat ttcagattat aatcaaccaa tttcaaacca tatgactagt
     7801 gaaaatcttg ttactgctcc tgtgggtacg gatcttgcaa cggctgagag tattcttcaa
     7861 gagcatcgta ttgaaaaact tccgttggtc gatgaagaag gcagtctttc tggtttgatc
     7921 actatcaaag atattgaaaa agttattgag tttccaaatg cggctaaaga tgagtttggt
     7981 cgtcttctag ttgcaggtgc agtaggtgtt acttcagata catttgaacg tgcagaggct
     8041 ctttttgagg caggagcgga tgcgattgtt attgatactg cacatggtca ttctgcaggt
     8101 gtcttgcgta aaattgccga gattcgtgct catttcccag atcggacttt gattgctgga
     8161 aatattgcta ctgctgaagg tgcacgtgcc ctttatgaag cgggtgtaga cgttgttaag
     8221 gttggtattg gaccaggttc tatctgtact actcgtgtga ttgctggtgt tggtgttccg
     8281 caagtaacag ctatctacga tgctgcagct gttgcgcgcg aatatggtaa aacgattatt
     8341 gctgacggtg ggatcaagta ttctggagat attgtaaaag cacttgctgc aggtggaaat
     8401 gctgttatgc ttggatctat gtttgctgga actgatgaag ctccaggcga aactgaaatc
     8461 ttccaaggac gtaaattcaa gacttaccgt ggtatgggat caattgctgc tatgaagaaa
     8521 ggttcaagcg accgttattt ccaaggttct gtcaatgaag caaacaagct tgttccagaa
     8581 ggaattgaag gtcgtgttgc ttataaagga gcggcagctg atattgtttt ccaaatgatt
     8641 ggtggtattc gctctggtat gggttactgt ggtgcagcta accttaaaga actacacgat
     8701 aatgctcaat ttattgaaat gtctggtgct ggtttgaaag aaagccatcc tcatgatgtg
     8761 caaattacta atgaggcacc aaattattct atgtaaaaaa caatgaaaag aactccagtg
     8821 aaaacaggag ttcttttaca atgttgtcaa tttccattta cagcagcttt accatcctga
     8881 atagtgaaga tacttagatt ttctggcaga ttttgaagat ggtctaagct tgttgttgtg
     8941 ataaaggttt ggattgattg agaaatcgtt tctaataatt ttaactgtct agtgttgtca
     9001 agttcactca tcacatcgtc aagcagtaat ataggagatt ctgtggtaat gctttccatt
     9061 aattcgattt ctgctaattt tatcgagagg acgagactac gatgttgacc ttggcttccg
     9121 aaactagcat ccatcccatt tatataaaaa gaaatgtcat ctcgatgagg accgacacca
     9181 gtattctttt taaataaatc tctggatcta cttttttcta aagcaatttt gaaagattcg
     9241 gataagtttt gtttgtcagt tatattgaca gaagattgat aggatattga caactcttcg
     9301 atctgattag agagttcaaa atgtttctta cgcccaaatg attctagttt ttttatgaaa
     9361 tctaagcggt gattcattac acgacatcca taatcaacta gctgatcatc taacacagaa
     9421 aggaatgttt catctatttt ttgagctgat tttaggtaag tgtttctttg ctttaggatg
     9481 tggttataat tggttaagtc agataaatag attggcttaa tttgcccaag ttccatatca
     9541 atgaattttc gtcgaatcga aggtgctcct ttaattagtt gtaaatcttc aggagcaaat
     9601 aagacaacat tcatgtgtcc tacataatct gaaaggcgtg cctgttttaa gtgattaact
     9661 tttgttacac gacctttttg tgttagttcg atttctaggg gaatggatcc agtttttttc
     9721 tgaacgagac ctgaaagatg aagttgttcc tcatcaaaat gaatgagatt tttatctgtt
     9781 cgagttcgat gactacgcgt taaggctaaa aaatagatag cctctaacat gtttgttttt
     9841 ccttgtgcat tacgtcccaa aaagacattc aatttaggat taaagtctat tttcgtttct
     9901 ttgtaattac gaaaagtctt gagagataga tgttgtagcc acatgattat ctaccaggga
     9961 atcgtggagc ttgttggctt ttaggtgatg aagcaggttt cgatttgtct tttttaactc
    10021 ccttattcat ctttttgaca agtttagcga tccgttcttt ttcaacttta tcagcttggt
    10081 attcatcttg ttcttcagaa gtaggttgtg tcaacaagat gtcaatgttc atgtcaggga
    10141 tgtcaacttt atcgccaata cgaagttttt ttccacgacg gctctctaat tccctattaa
    10201 agtaaacaga atgttcagag agaaatgatt taatagctcc tccgctatgt gtaattccaa
    10261 gttctttgaa tagtgcttgg agggtaataa attcttcaaa taatttgtat tccataattc
    10321 acctcaatct ttggtattat accatatttt cctaaaaata gtgagagaga cagcgagaaa
    10381 tgtaagcgat ttttatttca aaagtgttac agggaacaag aaaatttcag gttttcgtga
    10441 tataatagaa gtctgtatat aaggaggtaa atcatggagt tagtgcatgg aatttcaaca
    10501 cattttatcc aatcaaaaaa gtttaaaaca aacaaaatta ccgtgcgttt taccgctcca
    10561 ttatcccttg atacgattgc aggtcacatg ttgagtgcaa gtatgctaga gactgctaat
    10621 cagatgtacc ccacttctca agatttgagg agacacttgg ccagtctata cggtacagat
    10681 atgtcaacca attgtttcag aagagggcaa agccacatta tagaattgac atttacctat
    10741 gttcgtgatg agtttttaag taggaaaaac gtgctaacct ctcagatttt ggaacttgta
    10801 aaagaaactc ttttttcacc cgtagtagtt gataatgggt ttgatccggc cttatttgaa
    10861 attgagaaaa aacaattgct agcaagttta gcagctgata tggatgattc tttttatttt
    10921 gcacataaag aattggataa attgtttttt catgatgaac gtcttcaatt ggaatatagt
    10981 gatttacgaa atcgtatttt agctgaaact ccacaaagtt cttattcttg tttccaagaa
    11041 tttttagcca atgatcgaat agatttcttt ttcctaggtg attttaatga ggttgaaatt
    11101 caaaatgtat tagaatcatt tggctttaaa ggtcgaaaag gagatgtgaa ggttcagtat
    11161 tgtcaacctt attctaatat ccttcaggaa ggtatggttc ggaaaaatgt gggacaatcc
    11221 attttggaat taggttatca ttactgttct aaatatggtg atgagcaaca tttacccatg
    11281 attgtaatga atggtttact tggtggattt gctcactcta agctctttac aaatgtccgt
    11341 gaaaatgctg gattagctta taccatttca agtgagcttg atttatttag tggattcttg
    11401 aggatgtatg ctggtatcaa tcgagaaaat cgtaaccagg ctcgtaaaat gatgaataat
    11461 caactgcttg atttaaaaaa aggttatttt acagagtttg agttaaatca gaccaaggaa
    11521 atgattcgtt ggtcgttgtt actttctcaa gataatcaat cttcattgat tgaacgtgct
    11581 tatcaaaatg ccttatttgg aaaatcttca gcagacttta aaagttggat tgcaaagctt
    11641 gaacaaattg acaaagatgc tatttgtaga gtagctaata atgtgaaact acaagcgatt
    11701 tactttatgg aaggaataga atgacaaagg ttgtttttga agaaaaatac tatccagctg
    11761 taaaagaaat ggtttatcga actcgtttgg ccaacggatt gacagttgct cttttgccta
    11821 aaaaggaatt taaagaggtt tacgggagtg tcactgtaca gtttggttcg gtagatacgt
    11881 ttgtcacaga agttgacgga gatgtaaaac aatatcctgg aggaattgct cattttcttg
    11941 aacataaatt atttgagaga gaagattcta gtgatttgat gtcggctttt acgagtctag
    12001 gtgcagatag taatgccttt acaagcttta caaaaacaaa ctatcttttt tcagcaacgg
    12061 attatttttt agaaaattta gatttacttg atgaattggt aacatcagca cactttactg
    12121 aagcttccat tctgacagag caggatatta ttcagcaaga acgagaaatg taccaagatg
    12181 atccagattc gtgtttattc ttttcaactt tagcgaattt gtatcctggt acacctttag
    12241 caactgatat agttggaagt gaggagtcca tttcccaaat caatctaact aatttgcaag
    12301 aaaattttac aaagttttac aaacctgtaa acatgtctct gtttttagtt ggtaattttg
    12361 atgtggagcg agtacaggac tattttgaaa gcaaagaact gaaagattca gattttcagg
    12421 aagtagcaag agaaaagttg tttttacagc ctgtaaagcc aacagatagt atgagaatgg
    12481 aagtatcttc tcccaaacta gcgattggag ttagaggtaa gcgagaagtt tctgaagcgg
    12541 attgctatcg acatcatatt ttattaaaat tattgtttgc aatgatgttt ggttggactt
    12601 cggatcgttt tcaaaaatgt tatgaatcag gtaaaattga tgcgtcctta tctctggaag
    12661 ttgaaataac aagtcgcttt cattttgtca tgttgacaat ggatacgaaa gagccagttg
    12721 ctttgtctca tcaatttagg aaggctattc gtaattttac aaaggattta gatattacag
    12781 aggaacattt agatattatc aaaagagaga tgtttggcga atttttcagt agcatgaact
    12841 ctcttgaatt tattgcaacg caatatgatg cttttgaaaa tggtgagata atttttgatt
    12901 tgccgaaaat tttacaggaa attactttag aggatgtcct tgatgctgga catcatttaa
    12961 tagatgatgg tgacatagtt gattttacaa tattcccatc gtagtaacct attataatag
    13021 acactagaaa gaagggatga caagtatgag aaaaaaaaca attggagagg ttttacgatt
    13081 agctagaatc aatcagggat tgagtttaga tgaattgcag aaaaagacag aaatccagtt
    13141 agatatgttg gaagcaatgg aagcagacga tttcgatcaa cttccaagtc ctttttacac
    13201 gcgttctttc ttgaaaaaat atgcatgggc tgttgagtta gatgaccaaa ttgttttgga
    13261 tgcttatgat tctgggagta tgattactta tgaggaagta gatgttgatg aagatgagtt
    13321 gacaggtcgt agacgttcaa gtaagaaaaa gaagaaaaaa acatcatttt tacctttatt
    13381 ttattttatc ctttttgctt tatcgatttt aatttttgtg acttattatg tttggaacta
    13441 tattcaaact caaccagagg agccttctct ttctaattac agtgtggttc aatcaacaag
    13501 ttcaactagc tctgttcccc actcctcaag tagtagttct tctagtatag aatcagctat
    13561 aagtgtatca ggcgaaggaa atcatgtaga aatcgcttat aagacaagta aggaaacagt
    13621 taaattgcaa ttggcagttt cagatgttac aagttgggtc agtgtttcag aaagcgaact
    13681 tgagggcggt gtaaccttat cgccaaagaa gaaaagtgca gaagcaacag ttgcaactaa
    13741 aagtcctgta acaattacgt taggtgttgt aaaaggtgtt gatttgacag tagataatca
    13801 gactgttgat ttatcgaaat taacagctca gactggacaa atcactgtaa cctttactaa
    13861 aaattaagga aaaacgaatg aaaaaagaac aaattcccaa tctcttaaca ataggtcgaa
    13921 ttctctttat acctattttt atctttattt taacgatagg aaattcgata gagagtcata
    13981 tagttgcagc tattatcttt gctgttgcca gtattaccga ctatttagat ggatatttag
    14041 ctcgtaaatg gaatgtggtc agtaattttg gtaaatttgc agatcctatg gcggataagt
    14101 tactagttat gtcggctttt attatgttga ttgagttagg tatggctccg gcttggattg
    14161 ttgcagtgat tatctgtcgt gagttagctg tgacaggttt aaggctttta ttggttgaaa
    14221 ctggtggaac aattttagca gcagcaatgc ctggaaaaat taaaactttt agtcagatgt
    14281 ttgccattat tttcttgcta ttacattgga ctttgcttgg tcaagttcta ctttatgtag
    14341 ccttattttt cactatctac tctggctatg actatttcaa gggtagtgcc tatgtattta
    14401 aagggacatt tggttcgaaa tgaaatcaat aattgatgta aaaaatcttt cttttcgcta
    14461 taaagaaaat cagaactact acgatgtgaa ggatattacg tttcacgtga aacgtggaga
    14521 atggctttcg attgtagggc ataatggtag tggtaaatca acgacggttc gattaattga
    14581 tggcttactg gaagcagaat ccggagagat tgtaattgat ggccaacgtt tgactgagga
    14641 aaatgtttgg aatatacgtc gtcaaatcgg tatggttttt caaaatccag acaatcaatt
    14701 tgttggagcg actgttgaag atgatgttgc ttttggtttg gaaaatcagg gactttctcg
    14761 tcaagaaatg aaaaagagag tggaagaagc tctggcttta gttggcatgt tggactttaa
    14821 aaagagagag ccagcgcgtc tatcaggtgg ccaaaagcaa cgtgtggcca ttgcaggtgt
    14881 tgtagcccta agaccagcta ttttaatctt agatgaagca acgagtatgt tggatcctga
    14941 ggggcgtaga gaacttattg ggacagtaaa aggaattcga aaagactatg atatgacggt
    15001 catttctatt acccatgatt tggaagaagt cgccatgagt gatcgcgtat tggtcatgaa
    15061 aaaaggggaa attgaatcaa ctagtagtcc aagggagctt ttctctcgaa atgatttaga
    15121 tcaaattgga ttagacgatc cttttgctaa tcaattaaaa aaatctttga gccagaatgg
    15181 ctatgattta cctgaaaatt atttgacaga aagtgagcta gaggataagc tatgggaatt
    15241 gctctagaaa atgtgaattt tacatatcaa gaaggtactc ccttagcttc agcagctttg
    15301 tcggatgttt ctttgacgat tgaagatggc tcttatacag ctttaattgg gcacacaggt
    15361 agtggtaaat caactatttt acaactctta aatggtttat tggtgccaag tcaagggagt
    15421 gtgagggttt ttgatacctt aatcacctcg acttctaaaa ataaagatat tcgtcaaatt
    15481 agaaaacagg ttggcttggt atttcagttt gctgaaaatc agatttttga agaaacggtt
    15541 ttgaaggacg ttgcttttgg accgcaaaat tttggagttt ctgaagaaga tgctgtgaag
    15601 actgcgcgtg agaaactggc tctggttgga attgatgaat cactttttga tcgtagtccg
    15661 tttgagctgt cagggggaca aatgagacgt gttgccattg caggcatact tgccatggag
    15721 ccagctatat tagtcttaga tgagccaaca gctggtctag atcctctagg gagaaaagag
    15781 ttgatgaccc tgttcaaaaa actccaccag tcagggatga ccatcgtctt ggtaacgcat
    15841 ttgatggatg atgttgctga atatgcgaat caagtctatg taatggaaaa gggacgttta
    15901 gtaaaggggg gcaaaccaag tgatgtcttt caagacgttg tttttatgga agaagttcag
    15961 ttgggagtac ctaaaattac ggccttttgt aaacgattgg ctgatagagg cgtgtcattt
    16021 aaacgattac cgattaagat agaggagttc aaggagtcgc taaatggata gtatgatttt
    16081 agggcgttat atcccagggg attcgattgt tcaccgattg gatccacgta gcaaattgct
    16141 tgctatgatg ctactgattt tgattgtgtt ttgggctaat aaccccttga cgaatctgat
    16201 tctttttata gcgacaggga tatttattgc cttgtcagga gtatctcttt cattttttat
    16261 tcagggcttg aagtctatgt ttttcttgat tgccttcaca actatttttc aactattttt
    16321 catttctaat gggaatgttt tatttgagtt ttcgtttgtg agaatcacgg attatgcttt
    16381 gcaacaagct gggattattt tttgtcgctt tgtattgatt attttctttt caactttgtt
    16441 aaccttaaca accatgcctt taagtttggc atcagctgtc gaagctttat tagcaccttt
    16501 aaagcgtgtg aaagttccag ttcatgaaat tggattgatg ctgtctataa gtttgcgttt
    16561 tgtcccaacc ttgatggatg atacgactcg gattatgaat gcacagaaag ctcgtggagt
    16621 ggattttgga gaaggaagca tcgttcaaaa agtaaaggcg atgattccca ttttgattcc
    16681 tctttttgcg acaagtttaa aacgtgcaga ttccttggct atcgctatgg aagcgcgtgg
    16741 ctatcagggt ggaaaaggca gaagtcaata ccgacaattg aaatggactc taaaggatac
    16801 gctgaccatt cttgttattc tcgtacttgg ttgttgttta tttttcttaa aatcttagta
    16861 gctttcagga attgataaaa agttactgta acagtttttg atataaaggt agggatatga
    16921 accgttttaa aaaatcaaaa tatgtcatta ttgtttttgt cactgttctg cttgtgtcag
    16981 ctctcttagc gacgacttat tcaagtacaa ttgtgacaaa attaggagat ggaatctcat
    17041 tggttgatag agttgtacaa aaaccttttc agtggtttga ttctgtcaaa tcagatttgg
    17101 ctcatttgac acgaacatat aatgaaaatg aaagtttgaa gaaacagctt taccaattag
    17161 aagttaaatc aaatgaggtg gaaagtttaa agacagaaaa tgaacaactg cgccaattgc
    17221 ttgatatgaa gtctaaattg caagccacaa agactttagc agcagatgtt attatgcgtt
    17281 ctccggtatc ttggaagcag gagttgacct tagatgcagg tagatcaaaa ggtgcttctg
    17341 agaacatgtt agctattgca aatggtggct tgattgggag tgtttcaaaa gtagaggaga
    17401 actctactat agtcaacctt ctgacaaata cggaaaatgc tgataagatt tctgttaaaa
    17461 ttcaacatgg ctctactaca atttatggaa ttattattgg ctatgacaag gaaaatgacg
    17521 ttcttaaaat tagccaatta aatagtaata gcgatattag tgcgggagat aaggtgacta
    17581 ctggtggatt aggaaacttt aacgttgctg atattcctgt tggtgaagtg gttgccacaa
    17641 cgcatagtac agactatttg acacgagaag taactgttaa attgagtgca gatactcata
    17701 atgtagatgt gatagaatta gtggggaatt cataatgaga cagttgaagc gagttggagt
    17761 atttttattg cttcctttct ttgttctaat tgacgcccat attagccagc ttctgggctc
    17821 atttttcccc catgtacatt tggctagtca ttttcttttt ctatttctct tatttgagac
    17881 gatagaagta tcagagtatc tctacctagt ctattgtttt gttataggct tgatttatga
    17941 tgtttacttt ttccatctaa tagggattac aactctctta tttatcttat tgggagcctt
    18001 ccttcataaa ttgaatagtg ttattttgtt gaatcgttgg acaagaatgc tagctatgat
    18061 tgtgctgaca ttcctgtttg aaatgggtag ttatcttttg gcttttatgg tagggttgac
    18121 agtagatagc atgtcgattt ttatagtcta tagcttggta ccgacgatga ttttaaattt
    18181 tttatggatt actgtttttc aatttatttt tgaaaaatat tatctataag aacgacatat
    18241 aaatgtaaca aaggcgtaat atttattagg cctttttttg gtatactagt attgtcttta
    18301 aaagaaggag tatctacgta atatgaagaa aaaaatctta gcgtcacttt tattaagtac
    18361 agtaatggtt tctcaagtag ctgttttaac aactgcgcat gcagaaacga ctgatgacaa
    18421 aattgctgct caagataata aaattagtaa cttaacagca caacaacaag aagcccaaaa
    18481 acaagttgac caaattcagg agcaagtatc agctattcaa gctgagcagt ctaacttgca
    18541 agctgaaaat gatagattac aagcagaatc taagaaactc gagggtgaga ttacagaact
    18601 ttctaaaaac attgtttctc gtaaccaatc gttggaaaaa caagctcgta gtgctcaaac
    18661 aaatggagcc gtaactagct atatcaatac cattgtaaac tcaaaatcaa ttacagaagc
    18721 tatttcacgt gttgctgcaa tgagtgaaat cgtatctgca aacaacaaaa tgttagaaca
    18781 acaaaaggca gataaaaaag ctatttctga aaaacaagta gcaaataatg atgctatcaa
    18841 tactgtaatt gctaatcaac aaaaattggc tgatgatgct caagcattga ctacgaaaca
    18901 ggcagaacta aaagctgctg aattaagtct tgctgctgag aaagcgacag ctgaagggga
    18961 aaaagcaagt ctattagagc aaaaagcagc agctgaggca gaggctcgtg cagctgcggt
    19021 agcagaagca gcttataaag aaaaacgagc tagccaacaa caatcagtac ttgcttcagc
    19081 aaacactaac ttaacagctc aagtgcaagc agtatctgaa tctgcagcag cacctgtccg
    19141 tgcaaaagtt cgtccaacat acagtacaaa cgcttcaagt tatccaattg gagaatgtac
    19201 atggggagta aaaacattgg caccttgggc tggagactac tggggtaatg gagcacagtg
    19261 ggctacaagt gcagcagcag caggtttccg tacaggttca acacctcaag ttggagcaat
    19321 tgcatgttgg aatgatggtg gatatggtca cgtagcggtt gttacagctg ttgaatcaac
    19381 aacacgtatc caagtatcag aatcaaatta tgcaggtaat cgtacaattg gaaatcaccg
    19441 tggatggttc aatccaacaa caacttctga aggttttgtt acatatattt atgcagatta
    19501 atttacagag ggactcgaat agagccctct tttcaggttt taccgtgaca atccctatta
    19561 aaaattatat caaaatagct tgaaaatatt ggaaaagtat ggtagaatga aaattgtcgt
    19621 gtgaacgata atactcattc ttgatgaatt gtgaagcagt tgcccttggg tcgttttgcg
    19681 agttgaagtc aagaagagga aaaaaacaaa aaggagaaat actcatggca gtaatttcaa
    19741 tgaaacaact tcttgaggct ggtgtacact ttggtcacca aactcgtcgc tggaatccta
    19801 agatggctaa gtacatcttt actgaacgta acggaatcca cgttatcgac ttgcaacaaa
    19861 ctgtaaaata cgctgaccaa gcatacgact tcatgcgtga tgcagcagct aacgatgcag
    19921 ttgtattgtt cgttggtact aagaaacaag cagctgatgc agttgctgaa gaagcagtac
    19981 gttcaggtca atacttcatc aaccaccgtt ggttgggtgg aactcttaca aactggggaa
    20041 caatccaaaa acgtatcgct cgtttgaaag aaattaaacg tatggaagaa gatggaactt
    20101 tcgaagttct tcctaagaaa gaagttgcac ttcttaacaa acaacgtgcg cgtcttgaaa
    20161 aattcttggg cggtatcgaa gatatgcctc gtatcccaga tgtgatgtac gtagttgacc
    20221 cacataaaga gcaaatcgct gttaaagaag ctaaaaaatt gggaatccca gttgtagcga
    20281 tggttgacac caatactgat ccagatgata tcgatgtaat catcccagct aacgatgacg
    20341 ctatccgtgc tgttaaattg atcacagcta aattggctga cgctattatc gaaggacgtc
    20401 aaggtgagga tgcagtagca gttgaagcag aatttgcagc tttagaaact caagcagatt
    20461 caattgaaga aatcgttgaa gttgtagaag gcgacaacgc ttaatttata caaatagtaa
    20521 ttacctagga gggcggggct tagcccggct ctcctatttt caaaaaatat aggagaatta
    20581 aaatggcaga aattacagct aaacttgtaa aagagttgcg tgaaaaatct ggtgccggtg
    20641 ttatggacgc taaaaaagcg cttgtagaaa cagacggtga catcgaaaaa gcgattgaat
    20701 tgcttcgtga aaaaggtatg gctaaggcag ctaagaaagc tgaccgtgtt gctgcagaag
    20761 gtttgactgg tgtttatgtt aacggtaatg ttgcagcagt tattgaagta aacgctgaaa
    20821 ctgacttcgt tgcaaaaaac gctcaattcg ttgaattggt aaatactaca gctaaagtca
    20881 ttgctgaagg aaaacctgct aacaacgaag aagcacttgc tttgataatg ccttcaggtg
    20941 aaactcttga agctgcatac gtatctgcaa cagcaactat cggagagaaa atctcattcc
    21001 gtcgctttgc attgattgaa aaaacagacg cacaacactt tggagcatac caacataacg
    21061 gtggacgtat cggtgttatt tcagttgttg aaggtggaga cgaagcactt gctaaacaat
    21121 tgtcaatgca catcgcagcg atgaaaccaa cagttctttc ttacaaagaa ttggatgagc
    21181 aattcgttaa agatgagttg gcacaattga atcacgttat cgaccaagac aacgaaagcc
    21241 gtgcaatggt taataaacca gctcttccac acttgaagta tggatcaaaa gctcaattaa
    21301 ctgatgatgt tattgctcaa gctgaagctg acatcaaagc tgaattggct gcagaaggca
    21361 aaccagaaaa aatctgggac aaaattattc caggtaaaat ggatcgcttc atgcttgata
    21421 acactaaagt tgaccaagct tacacacttc ttgcacaagt ttacatcatg gatgacagca
    21481 agacagttga agcatacctt gaatcagtta acgcttcggt agttgagttt gctcgctttg
    21541 aagttggtga aggtatcgag aaagctgcaa acgacttcga agctgaagtt gcagctacaa
    21601 tggcagcagc cttgaataac taataataga aaggaagcaa attgcttcct tttttgtata
    21661 gggcttagat ttttcctgaa attgtttttt ccccagtctc actcgattag aataattaaa
    21721 acaaaaagcc ctataataag ggctaagtgc atttaggaga ctatacttca aattcatagt
    21781 aagcgcgtca taacaaggta tctatcctaa ttccccaact tccagtatac cagataaaaa
    21841 ataattatcg gaggaaaggt attgtactat gataattcaa ctaagtgatt taggtcaagt
    21901 tcaccttgtt tgtggcaaga cagatatgag gcagggaata gactcattag cctatgtagt
    21961 taaaacccac tttgaattgg atcctttctc cgatcaagtc tttctctttt gtggtggacg
    22021 taaagaccgc tttaaagttc tttactggga tggtcaagga ttttggatat tatataaacg
    22081 ctttgagaac ggaaaactga cttggtccag tacagaaaag gatgtcaaag ctctcgcacc
    22141 tgaacaagta gattggctga tgaagggctt ttctatcact ccccaaatat agtggattta
    22201 aactagaata gtacacctct gcttctaaaa cattgttaga aatcgatttg actatcctgt
    22261 tcttatttca ttttactata aattcatcag aaagtcgtga tttctattga aatgaggact
    22321 ttctttttat actcatctgt tttcaaaaag cattctagtc cgtctccgat taacgatgga
    22381 ctttatcacc tccttctcca gtccttgtat gacatcttcc aaagttcgaa aggctttatt
    22441 cttaaatcca cgtt
//
LOCUS       DAVHEX010000037        22386 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVHEX010000037 DAVHEX010000000
VERSION     DAVHEX010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22386
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.037"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(52..513)
                     /locus_tag="VVL45_001347"
     CDS             complement(52..513)
                     /locus_tag="VVL45_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU4187515.1"
                     /translation="MGKERREQGNLERKKRVVKKRKIPLEELKAFVEAHPDAFLREIA
                     ARFDCALPSVWAVLKQIKVILKKTTSFREQKPEKVSEFLDILDNLKDLPVLYIDETGI
                     NRYLYRPYAGAPRGEKVYDKISGRRFERTNEVEQKLNGSFLIRYIDSQIRE"
     gene            complement(480..623)
                     /locus_tag="VVL45_001348"
     CDS             complement(480..623)
                     /locus_tag="VVL45_001348"
                     /inference="COORDINATES: protein motif:HMM:NF013842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tnpB"
                     /protein_id="HEU4187516.1"
                     /translation="MAYSTDFKQGALDSIKGGHRHVEAAKVFDVGVRTLFTWEKKDVS
                     KGT"
     gene            892..1272
                     /locus_tag="VVL45_001349"
     CDS             892..1272
                     /locus_tag="VVL45_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU4187517.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            1371..1592
                     /locus_tag="VVL45_001350"
     CDS             1371..1592
                     /locus_tag="VVL45_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEU4187518.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            1609..1923
                     /gene="trxA"
                     /locus_tag="VVL45_001351"
     CDS             1609..1923
                     /gene="trxA"
                     /locus_tag="VVL45_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU4187519.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            2298..2762
                     /gene="queF"
                     /locus_tag="VVL45_001352"
     CDS             2298..2762
                     /gene="queF"
                     /locus_tag="VVL45_001352"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001852207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEU4187520.1"
                     /translation="MQHYETVEAVTFAYGQRHHLEIQITREIAKEQGIRHHILDMSLL
                     GQITAQPDFATIHISYIPDKLCVESKSLKLYLFSYRNHGDFHENCINTIGKDLVNLLD
                     PRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLAEQRLFQHDLYPEKIDNR"
     gene            2824..3216
                     /locus_tag="VVL45_001353"
     CDS             2824..3216
                     /locus_tag="VVL45_001353"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187521.1"
                     /translation="MFEVVDKTDEVSSKHCFEVADRTDEVSLKHCFEVADRTDEVSLK
                     HCFEVVDRTDEVSLKHCFEVADRTDEVSLKHCFEVADRTDEVSLKHCFEVADRTDEVS
                     NHTYDKVKLTWFEEIFEEYHTKKPCSSR"
     gene            complement(3241..3909)
                     /locus_tag="VVL45_001354"
     CDS             complement(3241..3909)
                     /locus_tag="VVL45_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEU4187522.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGFDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLAN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGPIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
     gene            complement(4020..4811)
                     /locus_tag="VVL45_001355"
     CDS             complement(4020..4811)
                     /locus_tag="VVL45_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HEU4187523.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLLDHLGDPSPEIRDDLVFTSF
                     AIGIQEELFTQEQFHFIAEGVSSDVGLDKEIDKVGLPTLERSFRALVYANLLYADANQ
                     QSVFYQGLQLEIRNVFSNQGLHYLSKEKDTTGFSSQYGWVHAFAHGADLLTEVVCHPD
                     FPINRIHEVFDILGQLFKRMSIRFTDDEDWRLARVIYEPILQGKLEQEQVASWIKTVD
                     FPIEEREDFSKFSNFRSCLVEVYVQLDQRNSLQDELKEAIQSFQY"
     gene            complement(5058..6854)
                     /gene="pepF"
                     /locus_tag="VVL45_001356"
     CDS             complement(5058..6854)
                     /gene="pepF"
                     /locus_tag="VVL45_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEU4187524.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYAFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVVADEEVLDRLGELPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKNSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIIAYSAELGE"
     gene            complement(6865..7608)
                     /locus_tag="VVL45_001357"
     CDS             complement(6865..7608)
                     /locus_tag="VVL45_001357"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEU4187525.1"
                     /translation="MQQYFVKGSAISPVTIEDKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
                     FDSIIVAYEESAKEGEAAALLQAVTGLETGSKLLFIFGPEGGLSPAEIESFEAKGAVL
                     AGLGPRILRAETAPLYALSALSVLVELEK"
     gene            complement(7610..8560)
                     /locus_tag="VVL45_001358"
     CDS             complement(7610..8560)
                     /locus_tag="VVL45_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEU4187526.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYVGNMD
                     RFGEIFPEVEQQEEIVVTAYYPDTVDVTVVEADLQARLAELTDFMDLGELKIGTTALA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEMIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNPG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLIDDAYRLVKDEGYLIMSGIIKDKW
                     DMVRESAESAGFFLETHMVQGEWNTCVFKKTKDISGVIGG"
     gene            complement(8698..9126)
                     /locus_tag="VVL45_001359"
     CDS             complement(8698..9126)
                     /locus_tag="VVL45_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU4187527.1"
                     /translation="MEIEISDFTGCKIALFCGDKLLTILRDDKASIPWANMWELPGGG
                     REGDESPFECVAREVYEELGIHLTEDCLLWSKIYPSMLFADKQSVFLVGQLTQNQFDS
                     IVFGDEGQGYQLMNVEEFLSSSQVVPQLQERLKDYLKVSD"
     gene            complement(9128..10195)
                     /locus_tag="VVL45_001360"
     CDS             complement(9128..10195)
                     /locus_tag="VVL45_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU4187528.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAIVFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQLKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENLLAELEKELAEGV"
     gene            complement(10214..10684)
                     /locus_tag="VVL45_001361"
     CDS             complement(10214..10684)
                     /locus_tag="VVL45_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEU4187529.1"
                     /translation="MATYGFLDILEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNAEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDTLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            complement(10975..11427)
                     /locus_tag="VVL45_001362"
     CDS             complement(10975..11427)
                     /locus_tag="VVL45_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEU4187530.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            complement(11464..11637)
                     /locus_tag="VVL45_001363"
     CDS             complement(11464..11637)
                     /locus_tag="VVL45_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEU4187531.1"
                     /translation="MTQKEMVKLLTAHGWIKTRGGKGSHIKIEKQGERPITILHGELN
                     KYTERGIGKQAGL"
     gene            complement(11780..11959)
                     /locus_tag="VVL45_001364"
     CDS             complement(11780..11959)
                     /locus_tag="VVL45_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187532.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSGPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            complement(12124..12362)
                     /locus_tag="VVL45_001365"
                     /pseudo
     CDS             complement(12124..12362)
                     /locus_tag="VVL45_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12632..13903
                     /locus_tag="VVL45_001366"
     CDS             12632..13903
                     /locus_tag="VVL45_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEU4187533.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYAARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQRIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKASGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDHLRKI"
     gene            14167..14239
                     /locus_tag="VVL45_001367"
     tRNA            14167..14239
                     /locus_tag="VVL45_001367"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14200..14202,aa:Lys,seq:ctt)
     gene            complement(14449..15768)
                     /locus_tag="VVL45_001368"
     CDS             complement(14449..15768)
                     /locus_tag="VVL45_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEU4187534.1"
                     /translation="MGDKKYTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEKNGVRQVQNMAFSDDGIHFEKIEQNPVATGADLPDELIA
                     ADFRDPKLFEKDGRYYSIVAAKHKDNVGCIVLLGSDNLVEWQFESIFLKGVEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVEWGEKCFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDTDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(15778..17394)
                     /locus_tag="VVL45_001369"
     CDS             complement(15778..17394)
                     /locus_tag="VVL45_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU4187535.1"
                     /translation="MKFKTFSKSAVLLTASLAVLAACGSKNTASSPDYKLEGVTFPLQ
                     EKKTLKFMTASSPLSPKDPNEKLILQRLEKETGVHIDWTNYQSDFAEKRNLDISSGDL
                     PDAIHNDGASDVDLMNWAKKGVIIPVEDLIDKYMPNLKKILDEKPEYKALMTAPDGHI
                     YSFPWIEELGDGKESIHSVNDMAWINKDWLKKLGLEMPKTTDDLIKVLEAFKNGDPNG
                     NGEADEIPFSFISGNGNEDFKFLFAAFGIGDNDDHLVVGNDGKVDFTADNDNYKEGVK
                     FIRQLQEKGLIDKEAFEHDWNSYIAKGHDQKFGVYFTWDKNNVTGSNESYDVLPVLAG
                     PSGQKHVARTNGMGFARDKMVITSVNKNLELTAKWIDAQYAPLQSVQNNWGTYGDDKQ
                     QNIFELDQASNSLKHLPLNGTAPAELRQKTEVGGPLAILDSYYGKVTTMPDDAKWRLD
                     LIKEYYVPYMSNDNNYPRVFMTQEDLDKIAHIEADMNDYIYRKRAEWIVNGNIDTEWD
                     DYKKELEKYGLSDYLAIKQKYYDQYQANKN"
     gene            complement(17423..18313)
                     /locus_tag="VVL45_001370"
     CDS             complement(17423..18313)
                     /locus_tag="VVL45_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU4187536.1"
                     /translation="MKNSIMDTKFDRRILLLNKIIIVFIVLMTLLPLLYIVVASFMDP
                     KVLVSRGISFNPADWTVEGYQRVFSDQSILRGFINSLLYSFGFAALTVLLSVFTAYPL
                     SKKDLVGRRWINYFLIVTMFFGGGLVPTYLLVKELGMLNTPWAIIVPGAVNVWNIILA
                     RAYFQGLPEELVEAAVIDGANDLQIFFKIMLPLAKPIMFVLFLYAFVGQWNSYFDAMI
                     YIKDPNLEPLQLVLRKILIQSQPGQDMIGAQAAMNEMKRLAELIKYATIVISSLPLIV
                     MYPFFQKYFDKGIMAGSLKG"
     gene            complement(18324..19241)
                     /locus_tag="VVL45_001371"
     CDS             complement(18324..19241)
                     /locus_tag="VVL45_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU4187537.1"
                     /translation="MNSKAKQVSLWERIKKQKLLLLMTVPGLVLTFIFKYIPMYGVLI
                     AFKDYNPLKGILGSDWIGFSEFTKFISSPNFGILLANTLKLSIYGLLLGFLPPIILAI
                     MLNQLLSEKVKKRIQLILYAPNFISVVVIVGMIFLFFSVGGPINNFLSMFGMKADFLT
                     NPDFFRPLYIFSGIWQGMGWASTLYTATLVNVDPALVEAARLDGANIFQRIWHIDIPA
                     LKPIMVIQFVLAAGGIMNVGYEKAFLMQTSLNLPTSEIISTYVYKVGLVSGDYSYSTA
                     VGLFNAVINVVLLVAVNQIVKRMNNGEGI"
     gene            complement(19392..20393)
                     /locus_tag="VVL45_001372"
     CDS             complement(19392..20393)
                     /locus_tag="VVL45_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU4187538.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLKKVEQ
                     AIKTLNYIPDYYARGMKKNRTETIAIIVPSIWHPFFSEFAMHVENEVYKRNNKLLLCS
                     INGTNREQDYLEMLRHNKVDGVVAITYSPIEHYLTSGIPFVSIDRTYSDIAIPCVSSD
                     NDAGGREAAKQLISKGCQHLAFVGGHNTTINETKRRRISFEKYVQEHKIPLSIFDLDE
                     TVTDYYGKLESFLEENSSIDGIFTINDFTALDVIEILEKNGKRIPHDVQIIGYDGIKM
                     AGDRDYLLSTIEQPLEEMAKEAVRILFDIIDGKTVNLQTILPVKFVEGKTTKNENKS"
     gene            complement(20755..22236)
                     /locus_tag="VVL45_001373"
     CDS             complement(20755..22236)
                     /locus_tag="VVL45_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU4187539.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSP
                     YDIIISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
BASE COUNT         6820 a         4833 c         3907 g         6826 t
ORIGIN      
        1 aaatcagcta atttttggtt tacattccta tttttatgca taattattct gtcattctct
       61 aatttgtgaa tcaatatatc tgattagaaa actaccattg agtttttgct ccacctcatt
      121 agtccgctca aaacgccgtc cgctaatctt gtcatagact ttctcccctc taggagcccc
      181 tgcataagga cgatagaggt agcggttgat tcccgtttcg tcaatatata ggactggtaa
      241 atcttttagg ttatccaaaa tatcaagaaa ctcagaaact ttctcaggct tttgttccct
      301 aaaactggtc gtctttttta aaatgacctt aatctgcttt aaaactgccc atacggaggg
      361 caaagcacaa tcaaaacggg ccgcaatttc ccgtaaaaaa gcgtctggat gagcctctac
      421 aaaggctttc aattcttcta aagggatctt acgctttttg acgactcgct ttttccgctc
      481 taagttccct tgctcacgtc tttcttttcc cacgtgaaga gagttctgac gccgacatca
      541 aaaactttgg ccgcctcgac atgtctgtgc cctcctttga tggaatctaa tgctccttgt
      601 ttaaaatcag tactatatgc catagcttta ttagaccatg cttattttaa actaagcaac
      661 tatagataga gaaatgaaat aaaatctgaa ttcgaatgtg ataaggagtt agaaattgaa
      721 acaataggat tcccccatta ttcacacaaa aataaaacca ctaaaaaaca gagcttgcca
      781 gcattcttga aaagtagtat aattattgca tgcgcaatca tcttgtgacg cagagactgc
      841 ccgttaatgg actttcttct attttatgac tctaaaaaga aaggagacac tatgacctat
      901 ttggaaaaat ggtttgactt caatcggcgt cagaaagaaa ttgaaagtct cttggaagag
      961 accattgccc aacagagcga gcaaagtctg accttgaaag agttctacct gctctactat
     1021 ctggacttgg ctgaagaaaa atctctacgc cagattgacc tgccagataa gcttcatctg
     1081 agcccgagcg cagtttctag gatggtggct cgcttggaag ctaaaaattg cggtctactc
     1141 agtcgcatgt gttgccatca agatagacgc tctagtttta tctgcctgac aaatgatggg
     1201 caaaagacac tggcctcgct acaaaagact gtcgaagaaa gcttggaagc aggtttggat
     1261 ttcttgattt aagatgattt ttaaaaaagt tgcgtgcgca atcatttttc ttgacattct
     1321 cttttataag gagtaaaata aagttagcat taaacaaagg agttttaaat atgattgaaa
     1381 ttacctatat agatgccagc aagaacgaaa gaactgtgac tttcgagtct tatgaagact
     1441 ttgaacgttc gcaacaagct tgccttatcg gcgtcgcaga ctactaccct gtccaaaaat
     1501 taacctacaa gggtcataat ttggactacc atgggactta tggagatatc ttcttctatc
     1561 tcatgaaaca agatttaagc caatataact aaaaaaagga gaaatacaat ggcaaaagca
     1621 attacagatg caacattcga acaagaaaca aaagacggtt tggtcttagt agacttctgg
     1681 gcaacttggt gtggtccatg tcgtatgcaa ggtccaatct tggacaaatt gtctgaagaa
     1741 ctttcagaag atgtcttgaa aatcgttaaa atggacgttg atgaaaatcc aaacacagct
     1801 cgtgcttttg gaatcatgtc tattccaact cttctcttca aaaaagacgg ccaagttgtc
     1861 aaacaagttg caggtgttca cacagcagaa caaatcaagg ccatcattgc tgaattgagc
     1921 taatcaaacg agagaccaag tactttcttt ggtctctttt ttcttgccct ttgccatttt
     1981 tcaaaaaata tgctagacta tagtagattg aaactagaat agtacatctc tacttctaaa
     2041 acattgttag aaatcgattt gactgtcctg atcgatttgt cctgttctta tttcacttta
     2101 ctatataaat agaatattac gatatttggg atatgccatc gctaaaaaaa acctcgattt
     2161 ggtatttttt agctccctaa tgggagcttt ttacgtgctc tgaacatttc ccattttgga
     2221 aggagtacta tgaaacgtca atcagccttg gtcgtcttag tggtggtcaa gattcaaaaa
     2281 cctgcctttt ctggaccatg caacactatg aaacagtcga agctgtcacc tttgcctacg
     2341 gccaacgtca tcacctcgaa attcaaatta ctagagaaat cgctaaggaa cagggcattc
     2401 gtcaccatat cctcgatatg tctctgctgg gacaaatcac tgctcagcca gactttgcga
     2461 cgattcatat ttcctacatt cctgacaagc tctgtgtcga gtcaaaatcc ctcaaactat
     2521 atctatttag ctaccgaaac cacggagatt tccacgaaaa ctgtatcaac accatcggga
     2581 aagacttggt caacttgcta gaccctcgct atttagaagt ctggggaaaa ttcactccgc
     2641 gcggtggcat ttcaatcgac ccctactata actacggtaa gcaaggaact aagtatgagg
     2701 gcttggcaga acaacgcctc ttccaacacg acctttatcc agagaaaatt gacaaccgct
     2761 aaagtcatac tcaatgaaaa tcaaagagca aactaggaag ttagccgcag gttactcaaa
     2821 gcagtgtttg aggttgtaga taaaactgac gaagtcagct caaaacactg ttttgaggtt
     2881 gcagatagaa ctgacgaagt cagcttaaaa cactgttttg aggttgcaga tagaactgac
     2941 gaagtcagct taaaacactg ttttgaggtt gtggatagaa ctgacgaagt cagcttaaaa
     3001 cactgttttg aggttgcaga tagaactgac gaagtcagct taaaacactg ttttgaggtt
     3061 gcagatagaa ctgacgaagt cagcttaaaa cactgttttg aggttgcaga tagaactgac
     3121 gaagtcagta accataccta cgacaaggtg aagctgacgt ggtttgaaga gattttcgaa
     3181 gagtatcata cgaaaaagcc ctgttcctca agatgaggag caaggctttt tgagtttcaa
     3241 ttattcttct gttccaagga aattttttgc aacaagggct gcaagaactc caccagcgat
     3301 tggtgcaagg atgaaaatcc aaacttgttg aagggctgcg ccgcctacca agacagctgg
     3361 tgccaagcta cgagctgggt ttactgaaag tccagtaatc ttcaatccga caagaatcat
     3421 cgccatcaat gacaaaccga ttaccaaacc agcaatcggg ccattgccct tgctttctga
     3481 agtcacagtc atgataacca atacaaacaa gaaagttgcg atgacttcaa acaagaaacc
     3541 accaaagaca gtgacaccgt ttgccaaggc attttcacca agactagcag ttgacatacc
     3601 tgagttagcc aagaggaaga agacagcgcc agaagcgatg aaagctccaa caacctgacc
     3661 aaggatgtag tttacaagtt ctgaagatga caaacgtttg tttacaaaca tagcaatcga
     3721 aacagccggg ttcaagtgag cacctgaaac agttccgatt gagtaggctg ccaccacgat
     3781 tgccaaacca aaggcaaagg cgattccaag gtgaccaagg ccatcaaaac catttccaaa
     3841 aacaacagct cctgtcccaa cgaacacaag catgaacgta ccgattaact cagcaacaaa
     3901 ttttttcatt ttctttctcc ttttttcaaa aactagatac tagtctatca aaagtaggaa
     3961 agggtttcaa gaaaattgat tggaaatttt ttgaaaatca tagaactatt agctaatctc
     4021 tagtattgaa aagactggat agcttctttc aactcatctt gtaaactatt tctctggtca
     4081 agttggacat agacttccac cagacaggat ctaaagttgg aaaatttgga aaaatcctcc
     4141 ctttcttcta tcggaaaatc aacagttttt atccaagaag ctacttgttc ttgctccaac
     4201 ttcccttgta aaataggttc atagatcact cttgctaaac gccaatcctc atcatctgta
     4261 aagcgaatcg acattctttt aaatagttgg ccaagtatgt caaatacttc atgaattctg
     4321 tttataggaa agtctggatg acagaccacc tctgtcagta aatcggctcc atgtgcaaaa
     4381 gcatgaaccc aaccatactg acttgagaaa cctgttgtat ccttttcttt tgaaagatag
     4441 tgcaagcctt gatttgaaaa gacattacga atttctaatt gtaatccctg ataaaaaacc
     4501 gattgctggt tggcatctgc atacaagaga tttgcataaa caagtgccct aaaagaacgt
     4561 tcaagtgttg gcagacctac cttatcaatc tctttatcta gtcctacatc agatgaaacc
     4621 ccttcagcaa tgaaatgaaa ttgctcctgt gtaaatagct cttcctgaat ccctatagca
     4681 aagcttgtaa aaacaaggtc atcgcgaatt tctggagaag gatctcccaa atgatcaagc
     4741 aaccactgga tttcctcctg gttataattt ggtttttctt ctgctatttt tcttagtaaa
     4801 tcttgataca tggtcaatac ctctacattt ctagcaactg ttcaaaaagg cagtcttaaa
     4861 tgactcaata ttgaattctc aattaaatac aatctgatat aaaatgacgt aaataactat
     4921 caataccagt tctacagtaa gttcaaattt aacatcacga ccttcaacga catttttgaa
     4981 aatagctaca actaagacaa atagaatgac gcttaacaag cccataaaca tcattctaaa
     5041 aaatttttct attcccctta ctcccccaac tcagcactgt aggcgataat ctggtcaact
     5101 gtgtcagaca agaattggat ggtatcacgg agtggtttgt ctgttgaaat atcagcaccg
     5161 ataatcatgg ctgactcaag tggtgtcttg ctaccacctg atttgaggag attgagccag
     5221 tcttcagctc cagtttctga gtttttaaga tgaaggtaac cagctgtcga gatgacaaga
     5281 ccagcagagt aagtgtaact atacaagccc atatagtagt gagcttggcg catccaagtc
     5341 aaagctgcat cgtcgtcgat ttcaatggca tcgccccaaa aatctgtcaa aacttccttc
     5401 ataatactgt tgagtttgct tgctccgaag gtttctcctt cttcaatcaa tgtatacacc
     5461 ttacgctgga aggcggcttc caagaggtgg gtgataaagt tatggaagta ggtgtctgtc
     5521 aagcgatgag ccagagcaaa gcgtttttga cgtgggtcat tagattggtt ctctaagtaa
     5581 tcactgagta gcaattcatt gaaggttgac ggtgcttcaa catagtaggt cgacatatgg
     5641 gcattgaagt aactttgatg attgtctgaa aagatgaatt gaccagaatg cccgatttca
     5701 tgaatcaagg tatagacatc gctcaaacgg cctgtccagc tcatgaggac ataagggtgt
     5761 acgcgatatg ggtccgccgc ataaccaccg gaatccttgc cactgttagc agcaaagtcc
     5821 acccagcgct cttcttggta acgagcaact tcctgacaat attcttgccc caaaggttct
     5881 accgacttca tgaccaaatc ataggcatcg tcaatagtca cttcaggatt cagggcgctg
     5941 tccaagtcca atttccagtc tgcaaaggtc atcttttcaa gaccatttac cttggcaaca
     6001 tgcttgaggt atctctgagc gactggtgca aagtccttca tgatgaggtc aatctggcgg
     6061 tcaaacatga cacggtccac ttcttgttca gctagaagat agtcaaagac agagtcgtat
     6121 cccttcatat cagccaagag tttttcagac ttgacctgag ccagataggc tgctgcagcc
     6181 gtattttggt gcttacgaag tccctctgag aaggaacgga aggatttctc acgaacctca
     6241 gcatcctcgt gattttggta gaaattctca taggtgacaa agctgttttt ataggtcttg
     6301 ccatgggctt caaagtcagc catttcaaaa tccccagctc gcatcttagt ataaatgtcc
     6361 tgcggactat agaaaacttc accgagattg gtcaaggcct tctccacatc agcccctaga
     6421 tagtgggctt ttttgatttt ggcctgacga atagctgccg tcaagtgagg caattcaccc
     6481 aaacggtcca agacttcctc gtctgctacc accaaggcat cgtcaaagaa ggtcaaggct
     6541 acgctggcat ctgtttcaaa ttccatccca gcttgggcaa tattggcaaa ttcgtcattg
     6601 ctatagtccg tcgtctgagg cataaaagca tagttgccaa tatggctcat ctgaatgtag
     6661 atctgttcca attccgcaaa ggccttctcg aaatcctcaa aagtatgaag attacccttg
     6721 tagtcacggc taaattggtt gatgtcttca cgagctttct cgattgcacg caagaaatcc
     6781 tcacggtctt ggtatagggc tgttaagtcc cagagttcct tctctggaaa ttctgaacgg
     6841 tgtttttgtt ccattttctt cctcttattt ctctaattct actaaaacac taagggctga
     6901 taaggcgtaa agcggtgctg tttctgctcg caaaatacga ggacctaggc ctgccaaaac
     6961 ggctccttta gcttcaaaac tttcgatttc tgcaggtgac agaccacctt ctggaccaaa
     7021 gataaagagc agtttggatc ctgtttcaag accagtgact gcttgcagaa gcgcagcggc
     7081 ttctccttct ttagctgatt cttcataggc tactatgata gagtcaaact ggtccagctg
     7141 agctagaaaa tctgcttttt tctcgaaaag tttaatactt ggtacaatat tacgcttgct
     7201 ttgctcggct gctccaaggg caattttttc tagtttttca acttttttac ccaatttctt
     7261 gccatcccac ttggcaactg accagtctgc aggaaaggcc cagatttggc tagcacccag
     7321 ttcggttact ttttgagcga tgaactccag cttgtctccc ttgggaaatc cagatgcgat
     7381 ggtcacttgg actggtagtt ccacattgtc atttaattct tggaccaact caaactgacg
     7441 attttccata tccagcacgc gcgccaagcg cttgatgcca tcatcaaaga ctaaggtaac
     7501 ctcatcctct tctttcaagc gcataacctg aaacatatgc ttactggttt ccttgtcctc
     7561 gatagtgaca ggagagatag cactgccttt tacaaaatac tgctgcatgc tagcctccaa
     7621 tcacaccaga gatatccttg gttttcttaa agacacaggt attccattcc ccttgaacca
     7681 tgtgagtttc gaggaaaaat ccagctgatt cagccgactc gcgcaccatg tcccacttat
     7741 ccttgataat gccactcatg atcaggtagc cttcgtcctt aaccaaacga taggcatcgt
     7801 ctattagatg aatgaggata tccgccaaga tattagccac aatcacatct gcctcaattt
     7861 ccacaccctt aagcaaatct ccagccgcta catggatatt ttccatgcca gggttgagct
     7921 caatattttc ctgagccaca cgaaccgcca catcatccag gtcataggcg aaaatttctt
     7981 tagcccccag aagcgagctg gcaatagaga gaacccctga accagtcccc acatctagca
     8041 ccgtttcgcc accacgaaga acctgttcca aggcaaaaag gctcatcttg gtagttgggt
     8101 gggttccagt accaaaagcc atgccaggat ccagcttgat aatcatttcc cccgcagtcg
     8161 cctcatagtc tgtccaagag ggaacgatgg tcaaatcatg agtgatacga gctggctcat
     8221 agtatttctt ccagttgtct gcccagtctt cctcagccaa ggcagtcgta cctattttta
     8281 actctcccaa atccataaaa tccgtcaatt ctgctaagcg agcctgcaag tccgcctcaa
     8341 ccactgtcac atccaccgtg tcagggtagt aggctgtcac tacgatttct tcttgctgct
     8401 ccacctctgg gaaaatctcg ccaaagcggt ccatatttcc cacatagtcc atgctgtctt
     8461 caatcgcaac accttgcgct cctagctcaa tcaagagatt ggaaaccaac tcctctccct
     8521 cacgcttcac tgtaactttt aactcttgcc atgtttccat tattaagata ccaagcccgt
     8581 aaaacacaaa accaaaatag gaaattctct gaagacgttt gtgtctaaga gaagtttatc
     8641 tttttggcac cgtgtttagg gcgggttcag tttagaaatt taaccgaacc atcctttcta
     8701 atcacttact tttaaataat cttttaatct ctcttgcaac tgaggcacaa cttgactgga
     8761 actaagaaat tcctcaacat tcatcagctg atagccctgt ccttcatctc caaatacgat
     8821 actgtcaaac tggttctgtg tcaactgacc aactagaaaa acggactgtt tgtctgcaaa
     8881 aagcatactt gggtaaatct tactccaaag cagacaatct tcagtcagat gaatgcccag
     8941 ttcttcataa acttcacgcg ctacacactc aaaaggactt tcatcacctt cacgtccacc
     9001 acctggcagt tcccacatat tggcccaggg aatgcttgcc ttatcatcgc gtaagatagt
     9061 caaaagctta tccccacaaa acaaagcaat cttgcaacct gtgaaatcag aaatctctat
     9121 ttccatatta gactccttcg gctaattcct tttccagctc tgctaacaag ttttcataat
     9181 agcgtttctc gtccctcaac attcgactac tatccatttt ctgtctagca atcttgagag
     9241 ccttacgagt tcgatctaca tctttcttca cctttaattg ataccaggct tgtatcactt
     9301 gaagattgga cagtttgaga gacagaaacg atttgacctg tcgaatactg gcatattgct
     9361 ccgcttgctc aaaatctcct tccaacaagg cgatatgaag cagggatagt tgggcaactg
     9421 tctgcatcat cggagtagtt gtcctctcaa gtaatgcttg aaactgctgt ttagctactt
     9481 cttccttccc ttccaaaatg gaaacttcac cttgcatacc taatacacca tccgcaaaac
     9541 tccctcgtgc atcctcagga actgcttgaa caaagtcttt caaatcatat tcttgaggag
     9601 ccagcaaagt ttgagcagaa tgtctcaata ccaggtaggc gtatttggta ttttcagggt
     9661 gttgtagtaa ttcccaaatt tttgctccat cggtgatgtc gactggcaaa atgttattta
     9721 ggaagaaaga taaattaaga aaaatccaag tccctgcaaa ataccagctt cttgtcaaaa
     9781 atccaaacac tatcgccaat aatatcaagc cgagatgaac catcaagcct cctgaaagca
     9841 tcaggatgat tctttgatcg ctttcatcct cttttaaacc aatgtattga gcaccaacat
     9901 ttttcagaat ggctgttcta ctaagatgaa acctgcctga ctttttggtc aaaataaaat
     9961 gtcctaatcc aaaagccacc agccgatagc ctgtcaagta gccacaaaaa gcatgaccca
    10021 gctcatgaag aataaagatt aaatacatgc ttagaagagc gaaggcataa ccaaaagtaa
    10081 aggctaaaac tgcggaatac cccaactctg caaatgcgat tgttccacaa gcaaaagcta
    10141 gcataataaa gacaacagct agcacataaa ccaaataagt cccaattttc ttcataacac
    10201 ctccaaccaa ctcctagtat cttggataag gataaaattc tccctcttcc aagccaactt
    10261 ttccttcttc aaagacttct tgattccatt ccataacaaa ttcctctgct tctggatctt
    10321 ccaaaaagtc catgagggta tctagcccaa cctccgcagt atctttgagg aaaagggcaa
    10381 aataagctaa aaactcgcga gaaaatcctt ttttaggtag ataagggata accgttaaat
    10441 agtcttctgc attgacggtt gacttggcag gattgtagaa aaggaccgct tcctcaaaaa
    10501 gaatgtcatc tgatgaaacc tctccgtctt catccaccat ctccacacct gcagcatttt
    10561 gcgcttccaa tagaaaactc acttctaccg cgtggttacg cttgtcccaa ctaatctcaa
    10621 agtcaaaggg aaagttcttg tccaactctt cctctaaaat atctaaaaat ccgtatgttg
    10681 ccattttgtc ctctttctat gcgactcttt aatcgccccg attgctcgga aatatgctaa
    10741 aatagatact accatcttac cacaaaatta ttttatgtcc taattatacc atattacctc
    10801 atttaaaccc ttggtatcag tgattttctt aaaagtctga tttcttcatt tctcataaaa
    10861 atcaatataa aaagccctcg aaagggctaa taaatctata aaatcaatag gcgagtaact
    10921 agcacaagtg gacgtgcttt tttattgact attaccacga taccacgctt aatcttaggc
    10981 ttgaactttc ttatctgcaa tagcgtctgt caaagtctga gaaaagttaa gtcccatttc
    11041 tcgtcccaac ttatctgccc attttggtat ggtcaaagtc tttttaatgg gttcctgact
    11101 tcctaggtat tctgatacat caacagatac catagaaata aaagatttat caaggtcata
    11161 ggttgacacg aaatcttcat catctttaaa aggatcatta tcaattaaag acaagctatt
    11221 gatatctgat ggctgaggta actctccatc actctctatc aaatctgcaa cagttatccc
    11281 tagccactcc gaccccatag ccaaagcctc agaaatcccc tctccttgtg tagctgagta
    11341 ttcaaaatct gggaaatgga caaaataagt cgcttctgtt ccgtctgtgt cgtcataata
    11401 aaataaagct ggatacgtaa ctaacatttc actacctcca tatcaaaaag cagggactga
    11461 attttacaac ccagcttgct ttcctatccc tctttcagtg tacttattca gctcaccatg
    11521 aaggattgtg ataggtcttt ccccttgctt ctcgatttta atatgggagc ctttaccgcc
    11581 tctagtcttt atccaaccat gggccgtaag gagtttaacc atctcttttt gtgtcatagg
    11641 tatagcgctt ttacctcctg acaacaccat tataacacgt gttacacgta ttgtaaagga
    11701 gtgatactta ttattctatt atacataaaa gcccctagat gtggttctaa gggaaaccaa
    11761 tttattcata cctatttttc taatgagtag taaaaactgc ttctttatcg agcaattcat
    11821 catctgtata gtcaattgta aaagtatctc gatctaagac agattgaggc ggagttgaat
    11881 gaatcatagg accactgcgt actctatatt ttttatctcc aatttttaca aactgatact
    11941 cttcgaaaat caaattcaaa ccacgtcaac gtcgccttac cgtactcaag tacagcctgc
    12001 ggctagtttc ctagtttgct ctttgatttt cattgagtat gattaactct caagtcttcg
    12061 aaatcaggat tttcaacagt tattacaagg aggcgattta ctacttcaaa aacatcaatt
    12121 attctatttt tcatattttt tcaacccatt attagaatga acttcttggt aagcaaaatc
    12181 aagtttagat ttaatgtttt cgtacaaatc taaaatctct tttggagtat cttccggaag
    12241 aaaagttttc ttttccctga aataacttga tcactaagaa tccaatgacg aatttgtttt
    12301 gtaaaaatca aaatttcctg acttggtcca tcatttccat tgcttatcac ctctcttttc
    12361 attatagttc atacaatgac attcagcaat attatttctc aagtcagcac ttccacttct
    12421 ttaggctcaa ctatcctatt ttgagcttta aggaaaatca aatctctcat gctgatacct
    12481 ctcctcatta aattaaatag taaaaaagat tctatctcac tccctgatta ttacaaaacc
    12541 attgaaatat cacaactaat aggctagaat ggacatagta agatatagta gatgagtcat
    12601 tctactcaaa tccacgctag aaaggactgc tatgccagac aatctcgcgc ttcgcatgcg
    12661 ccctaaaacc atcgaccagg tcatcggtca ggagcatctg gtcggacctg gaaaaatcat
    12721 ccgccgcatg gtggaagcca accgcctgtc ctccatgatt ctatatggcc ctccgggaat
    12781 cggcaaaacc agtattgcct ctgccatcgc tggaacgacc aagtatgcct ttcgaacttt
    12841 caatgcgaca gttgatagta aaaagagact gcaagaaatc tcggaagaag ctaaattttc
    12901 tggtggtctc gtcctattgc tagacgaaat tcatcgacta gataagacca agcaagactt
    12961 cctcttgcct ctcttggaaa gtggactggt catcatgatt ggagcaacga ctgaaaatcc
    13021 tttcttttcc gtcactcctg ccattcgtag ccgtgttcaa atttttgagt tggaacctct
    13081 gtctaaccaa gacgtcaaag aggccctgca gatagctcta agtaaccctg aacgtggttt
    13141 tgattttcca atagaactag atgaggatgc gctggatttc atcgcaacct ctacaaacgg
    13201 agaccttcgc tctgccttta actcactgga cttggctgtt ctctctaccc ctgagaatga
    13261 cgagggcatt cgccacatca ccttagacat catggaaaat agtcttcaga gaagctacat
    13321 cactatggac aaggatggag acggtcacta tgatgttcta tctgccctgc aaaagtctat
    13381 ccgtggctca gatgtggatg ccagtctcca ctatgctgcc cgcttgattg aggctgggga
    13441 tctgcctagt ctcgctcgtc gcttgactgt tatcgcctat gaagatatcg gtttagccaa
    13501 tcctgaggcc cagattcata ccgtgactgc tctggatgct gcacaaagga ttggtttccc
    13561 agaagcccgc attctcatcg ccaatgtcgt gattgatttg gccctttctc caaaatccaa
    13621 ctcagcctat gtagctatgg ataaggcact tgctgacctc aaagcatcag ggcacttgcc
    13681 tattccgcga cacctgcgtg atgggcacta cagtggaagc aaggaactgg ggaatgccca
    13741 agactatctc tatccacaca actatcctgg aaattgggtc aagcaagact atctgccaga
    13801 aaaaattcgt aatcatcact atttccaagc agaagatact ggtaaatatg aacgggcttt
    13861 ggctcaaaga aaggaagcta tcgaccattt gcgaaaaatc tgaaatcctt ttcaaaaaac
    13921 tgcactttcc tcttgatttt ttttgaaaaa gtggtatcat ataaatatag aaacgctgtg
    13981 gtgtacgact tcacacttaa gtgttgaccg actatttttt gtattattag ggaaacaaaa
    14041 gtcttctaac agcatgtagg ccgtctcaca cggaaacagc ttcagttata gcgagttgcc
    14101 cacctgctta attgcgcggg ttcaatacaa accgtgaagt ttcggcacca atacagcttt
    14161 ttatttgcct tcttagctca gttggtagag cagtagactc ttaatctatg ggtcgcaggt
    14221 tcgagccctg cagggggcat ctaaatcaac aggaaaaagc cttataatat agggcttttt
    14281 ttgcttaaat aaaatttttt tcaaattctt tagaaaacta gtttaaatca taatccaaca
    14341 ccaattttac aaataccata aactaagctt atatacccaa aacacaaaaa gtcctctttc
    14401 cactatttta gaaagagaac tttttcttta ggagaaataa cttaattttt aatcaattcg
    14461 tgatagctca gctggcaccg ttaagtaata agttgcagtc aagcttgctt caccttgatt
    14521 gacaaaaatc tcgatggaat ttttatctag aacaacttcc aattctttag cttcaatatc
    14581 tacataccgt cgactagtgt cctgttcttc ttcacctaga attttttgaa taagatggct
    14641 acgatcaatg taaacttgct gcgcattact gtcataacca aattcaagat aatctgtatc
    14701 atttcctaag tggtaatgac aatctttatc tatttggatt tgatattggc cttttttaac
    14761 agggaattgt cttagtttgc catcttccaa tcttagaatt ctaggtaaag tcatggcaca
    14821 tgcccacttg tgttcttggt catgggttgg aagggtacgc ccccatgtct gcatccaagc
    14881 aatcaggata cgacgatttt gatcgtccaa caatgtttga ggcgcataga agtcttggcc
    14941 atgatcaatt tcttgaactg attctgggat aaaacatttt tctccccact ctaccttacc
    15001 cgtgaacaaa agcgatgagt tgatgttatg atatgagtct ccctcacgct gataacgcat
    15061 gggtgacata ataaggcaat ctttcccatc taactcgaag taatctgggc attcccacat
    15121 aaacccttgg tgttctaccc cttttaaaaa gatggattcg aactgccatt ctactaggtt
    15181 atcggaccct agtagaacga tacagcccac attatccttg tgtttggcag ctactatgga
    15241 gtaatagcgt ccatcttttt caaagagttt tggatcacgg aaatcagcag caatcaactc
    15301 atctggcaag tctgctcctg ttgcaactgg attttgttca atcttttcaa agtgaatccc
    15361 gtcgtctgaa aatgccatat tttgcacttg gcggacaccg tttttttctt cgatatgccc
    15421 agtgtacatg agccagaggc gatcatcctt gacaatggca gagcctgaga aacaaccatt
    15481 tcggtcataa tcttgatcag gagcaagtgc cactggcaag tgctcccaag tcaccaagtc
    15541 cttactttta gcatgtcccc agtgcatagg tccccaaaca ctatcatatg gatagaattg
    15601 ataaaagaga tggtattctc cacgaaaata aacaaatcca tttggatcat tgatccaacc
    15661 aatctcagct gaaaaatgtt cttcaggctt atattgagta ttaacgagat gtttattttc
    15721 tgctataaaa cgattggctt tttctactgt gtatttctta tctcccataa tcaacctcta
    15781 gtttttgttt gcttggtatt ggtcgtagta tttttgttta atagcgaggt aatcagaaag
    15841 tccgtatttt tcaagttctt tcttgtaatc atcccactca gtatcaatat tgccatttac
    15901 aatccattca gcacgtttac ggtagatata gtcattcata tctgcttcga tatgggcaat
    15961 cttgtccaaa tcttcctgtg tcataaagac tcttggatag ttattgtcat tgctcatgta
    16021 aggaacataa tattctttga taagatccaa acgccatttg gcatcatcag gcatggttgt
    16081 tactttacca tagtatgaat ctaggatagc tagtggtcct cctacttcag tcttttgacg
    16141 aagttctgct ggtgcagttc cgtttagtgg taagtgtttt agactatttg acgcttgatc
    16201 caattcaaag atgttttgtt gtttgtcatc tccgtaagtt ccccagttat tttgcacaga
    16261 ttggagtgga gcgtattgtg catcaatcca tttagctgtc aattctaggt ttttgtttac
    16321 actggtaata accatcttgt cacgtgcaaa tcccataccg tttgtacgag ctacgtgttt
    16381 ttgaccactt ggtccagcaa gtactggtaa aacatcataa ctttcgttac ttccagtaac
    16441 attattctta tcccatgtaa agtaaacacc aaatttctga tcatgacctt tagcaatgta
    16501 actattccaa tcatgttcga aagcttcttt atcaatcaag cctttttctt gcaattgacg
    16561 gataaatttg acaccttctt tatagttatc gttatctgct gtgaaatcaa ctttgccatc
    16621 atttcctact actaaatgat catcgttatc ccctatacca aatgcagcaa ataggaattt
    16681 aaaatcttcg tttccgttac cactaataaa tgaaaatgga atttcatcag cctctccatt
    16741 tccatttgga tccccgtttt tgaaagcttc taggacttta atcaaatcat cagtagtttt
    16801 tggcatttca agaccaagtt tcttaagcca atctttgtta atccaagcca tatcgttgac
    16861 gctgtgaata gactctttac catctccaag ctcttcaatc catgggaatg agtaaatgtg
    16921 cccatcaggt gctgtcatca aggccttgta ctctggtttc tcatccaaaa ttttcttaag
    16981 atttggcatg tatttatcaa tcaaatcttc aactggaata ataacacctt ttttagccca
    17041 gttcatcaag tccacatctg aagctccgtc gttgtggata gcatctggta aatcaccact
    17101 agaaatatcc aagttacgtt tttctgcaaa gtcggattgg tagttggtcc agtcaatatg
    17161 aacgccagtt tccttctcca aacgttgcaa aattaacttt tcatttgggt ctttaggaga
    17221 taacggtgaa ctggctgtca taaacttcaa tgttttcttt tcttgaagcg ggaatgttac
    17281 accttccaac ttataatctg gacttgaagc tgtatttttt gagccacagg ctgcaagtac
    17341 tgctaaacta gctgtcaaca aaactgctga ttttgagaat gttttgaatt tcatgagaaa
    17401 actcctttta ttttttcttt ttttatcctt taagtgaacc agccataatt cctttatcaa
    17461 agtatttttg gaagaatgga tacataacaa tcaatggcaa gctggaaatg acaatagttg
    17521 cgtatttaat caattcagct aaacgtttca tttcattcat agccgcttgt gctccaatca
    17581 tgtcttgacc tggttggctc tgaatgagaa ttttacgaag tacaagttgc aatggttcca
    17641 agtttggatc cttgatataa atcattgcat caaagtatga gttccactgt cctacaaaag
    17701 cataaaggaa gagaacaaac ataattggtt ttgcaagagg aagcatgatt ttgaagaaaa
    17761 tctgtaaatc atttgcacca tcaatgacag cagcttcaac taattcttca ggcaatcctt
    17821 ggaaataggc cctagcaaga ataatattcc aaacattgac agcacctgga acaatgatag
    17881 cccatggagt attgagcatt cccaattctt ttacgagcaa gtaagttggg actaaaccac
    17941 caccaaagaa catagttaca atcaagaagt agttaatcca acgacgtcca accaagtctt
    18001 tcttagaaag aggataagct gtaaacacag atagcaagac tgttaaagct gcaaatccaa
    18061 aagagtatag tagagaattg ataaaacctc ttagaataga ttggtcactg aatacacgct
    18121 ggtaaccttc tacagtccaa tcggctggat taaagctaat ccctctacta accagaacct
    18181 taggatccat aaaggatgct acgacgatat aaagtaaagg aagcaaagtc atcaaaacga
    18241 taaagacaat aatgatttta tttaagagta agatacgtct atcaaatttt gtatccataa
    18301 tcgaattttt catactttcc tccttaaatt ccttcaccat tattcatgcg tttaacgatt
    18361 tggttaactg caacaagcaa tactacgtta atcactgcat taaacaaacc aaccgctgtt
    18421 gagtaagaat agtctcctga tacaagacca actttataga catatgtcga gataatttca
    18481 gaagttggca aatttaacga tgtctgcatc aagaatgctt tttcatatcc gacattcata
    18541 attccacctg cagctaaaac aaattggata accataatag gcttaagagc tggaatatca
    18601 atgtgccaga ttcgttggaa gatattggct ccatccagtc gagctgcttc tactaaggct
    18661 ggatctacat ttaccaatgt tgccgtgtag agcgttgaag cccagcccat tccttgccag
    18721 ataccactaa agatgtataa gggtctaaag aagtctggat ttgtcaagaa gtcagccttc
    18781 attccaaaca tagaaagaaa attgttgatt ggtcctccca ctgaaaagaa gaggaaaatc
    18841 ataccgacaa taacaacgac tgagataaag tttggtgcgt ataaaatgag ctgaattcgt
    18901 tttttgactt tttcactcaa gagttgattg agcataatcg caagaatgat tggtggtaaa
    18961 aagccaagca ataaaccata gatacttaat tttaatgtgt tggctaacaa gataccaaag
    19021 ttgggagagg atatgaattt tgtaaactca gaaaaaccaa tccaatcact ccctaaaatt
    19081 ccttttaaag gattgtaatc tttaaatgcg attaaaaccc catacatagg gatgtattta
    19141 aagataaagg ttaaaactaa accggggaca gtcatcaata acaagagttt ttgtttcttg
    19201 attctttccc aaagagaaac ttgcttcgct ttgctattca tagcaacctc ctttttttga
    19261 aacgtttcat tttttctata aaagatagcg cttggcgctg tctttttatt tctttttttg
    19321 aaacgtttca tttttgtttt aaaaataaat ctttttatct gaattcattc tatcattgat
    19381 agcgctttct gtcaagactt attttcattt tttgttgttt ttccttcaac aaattttact
    19441 ggtaggattg tctgcaaatt aacagtcttt ccatcaatta tatcaaacaa aatacgaacc
    19501 gcttcttttg ccatttcttc caatggttgt tcaatagttg aaagtagata atctctatct
    19561 ccagccattt taattccatc gtatccaata atttggacat catgaggaat acgtttgcca
    19621 tttttttcta gtatttcaat cacatccaaa gctgtaaaat cgttgattgt aaaaattcca
    19681 tctatagaag aattttcttc caagaaactc tctagctttc cataatagtc agtaactgtc
    19741 tcatccaaat caaaaatact aagaggtatt ttatgctctt ggacatactt ttcaaatgaa
    19801 attctacgac gtttggtttc attaatggtt gtattatgtc cgcccacaaa ggctaaatgc
    19861 tgacatcctt tactaattaa ttgtttagct gcttctcttc cacctgcatc attatctgat
    19921 gaaacacaag gaatggcaat atctgagtat gtgcggtcaa tactaacaaa gggaattcct
    19981 gacgtcaagt aatgttcaat tggactatag gtaatggcaa ccactccatc aactttatta
    20041 tgacgcaaca tctccagata gtcttgctct ctatttgtac catttataga acataagagt
    20101 aatttgttat ttctcttata gacttcattt tccacatgca tagcaaattc tgaaaagaag
    20161 ggatgccaga tacttggtac aatgattgca atcgtttctg ttcgattttt tttcattcct
    20221 ctagcatagt aatctggaat gtaattcaaa gttttaatcg cttgttccac ttttttcaaa
    20281 gttacttctt taatgccttt ttctttatta attacacgtg aaacagttcc aacactaact
    20341 cctgcttcta aagcaacatc tttcatggta attgattttc tttgttctac catattatca
    20401 cctcctttca atatatagta tcatgcaaat gctttttaag caactatttc tcaatcattt
    20461 ttggccagat catttatccc atcatgaata aaatcactcc aattagcttt tgaaaatact
    20521 tcaattttca tgtgtaaaca tctacataaa acaggaaaag ccttggtttt atggcttttt
    20581 tcgtatcttc tacaaaaaaa gcaagagttt tagagggcta taaatctaga tgtacatttt
    20641 gcttaaatga ttgaaggtct tttcttaaca aaaacacccc aaaaattaga ttttttctgt
    20701 ctaacttttg aggtacagtt caaacgcgaa atagcgtttt tttgttattt ttggttactc
    20761 atctaatcga ataaacatca tggcatttaa caagtatatg agtgagaccg tgtttatatt
    20821 atttgaatag atgagtctct tattttcaat aggaggaata ataaaattag aaataatgat
    20881 atcataaggt gaatcttcta aagattcctt tgataattct aattcagtcc aaacttccag
    20941 ttcaaaatta ttgctacagt aataagaaag tgtctctgca acgaattttg catgatactg
    21001 atcaaaatta ctcataacta aaacctttag tttaggctga ttttgtagca aattaatcac
    21061 caaatgtttg gtatgagtga tgaaggtata agatagatga tttaccatca ttgaactaga
    21121 acaaacctca agagtctcta aatagtgaga aagctctttt tttatatctg aaacaaattt
    21181 tggaaaaata ttttgaaagt tcctgattgt attccctttt tgatcaaata aaataaactc
    21241 agtaaacaac tcttgacgat acagatgtgc ggtattatgc agatgccaaa tcagattatc
    21301 cttattctcc atttcaatct gatacttgac tgaaatctga tcaataaaat cactcaatag
    21361 atggtaagat ttttcaacat aactatcctt ttttacgcat ttcataaaga gactttcatc
    21421 tatgaaaaac attttttgaa agtaagacac aaataattgg caaacaactt cttcatctaa
    21481 agagatattg tattctgatt caaaactctg agcaacacct tctattcctt ctgcctgcat
    21541 taaaaaatcc aaactttggt cgttaaaaga atctttatct acttccataa aatgaccaaa
    21601 ctttattcta tataggttcg taactaggag caactttagc attctatgcg ttgacaaatt
    21661 cattggaaag cttgtttcct tataaaccaa ttctaacaat tgagatagtg gctctgatga
    21721 aaaattttca aatggccatt ctaggaaata atatttttct gaaaaatatt gtgcaaaaaa
    21781 gtaacgaatg tctctctcat ttccaatgat ttgaacaggg gtcagactga cttcaaattg
    21841 aaattgcctt ttaatcactt tattgatttg gctaataata cgatagagcg aagatgaact
    21901 gatataaaat tctttacaaa tactctcagc ttgacaacct tcattaaaga agatgaattc
    21961 taaaatcgaa aaatgagttg aatgtttaaa gaaatgatgg taaaccattt caatatcact
    22021 atcatcggta ttaataatgc gtataccatt agtagaagaa tgaaaaatca agtcaggaaa
    22081 agcagattta acatgggata gatcatcttt gactgcacgt tctgtacaat ttaataactc
    22141 tgctagttca gaacgatgaa accaacgttt atgttcaaat aataattcta ataattctaa
    22201 ttgcctatga ctttttttag ataataaatc tctcatgaat atctttctct ctttataaat
    22261 tatcggatta aacctcttgc aattatacca caaagaatag gtatagcatg atataacgac
    22321 ttttcctaaa atcttttatt tcgtataata acactacgga gacaatatat aaacaatttt
    22381 cttatt
//
LOCUS       DAVHEX010000038        22181 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVHEX010000038 DAVHEX010000000
VERSION     DAVHEX010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22181
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.038"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(100..324)
                     /locus_tag="VVL45_001374"
     CDS             complement(100..324)
                     /locus_tag="VVL45_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory protein Asp5"
                     /protein_id="HEU4187540.1"
                     /translation="MIDILIVLAIILSLALIVLVTIQPRQNQLFSMDATSNIGKPSYW
                     QSNTLVKVLTLLVSLALFVLLLTFMVITYK"
     gene            complement(329..508)
                     /gene="asp4"
                     /locus_tag="VVL45_001375"
     CDS             complement(329..508)
                     /gene="asp4"
                     /locus_tag="VVL45_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory system protein Asp4"
                     /protein_id="HEU4187541.1"
                     /translation="MSEEDLFYKDVEGRMEELKQKPIKKEKETRGEKISKTFSLLLGL
                     MILIGLLFTLLGILR"
     gene            complement(510..1847)
                     /gene="gtfB"
                     /locus_tag="VVL45_001376"
     CDS             complement(510..1847)
                     /gene="gtfB"
                     /locus_tag="VVL45_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HEU4187542.1"
                     /translation="MIELYDSYSQESRDLHESLVATGLSQLGVVIDADGFLPDGLLSP
                     FTYYLGYEDGKPLYFNQVPVSDFWEILGDNQSACIEDVTQERAVIHYADGMQARLVKQ
                     VDWKDLEGRVRQVDHYNRFGTCFAKTTYSADSEPIMTVYQDVNGQQVLLENHVTGDIL
                     LTLPGQSMRYFANKVEFITFFLQDLEIDTSQLIFNTLATPFLVSFHYPDKSGSDVLVW
                     QEPLYDAIPGNMQLILESDNVRTKKIIIPNKATYERALELTDEKYHDQFVHLGYHYQF
                     KRDNFLRRDALILTNSDQIEQVEAIVEALPDVTFRIAAVTEMSSKLLDMLRYPNVVLY
                     QNASPQKIQELYQLSDIYLDINYSNELLQAVRQAFEHNLLILGFNQTVHNRLYIAPDH
                     LFESSEVAALVETIKLALSDVDQMRQALGRQGQHANYVDLVRYQETMQTVLGG"
     gene            complement(1840..3351)
                     /gene="gtfA"
                     /locus_tag="VVL45_001377"
     CDS             complement(1840..3351)
                     /gene="gtfA"
                     /locus_tag="VVL45_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HEU4187543.1"
                     /translation="MTIYNINLGIGWASSGVEYAQAYRAGVFRKLNLSSKFIFTDMIL
                     ADNIQHLTANIGFDDNQVIWLYNHFTDIKIAPTSVTVDDVLAYFGGEESHREKNGKVL
                     RVFFFDQDKFVTCYLVDEDKDLVQHAEYVFKGNLIRKDYFSYTRYCSEYFAPKDNVAV
                     LYQRTFYNEDGTPVYDILMNQGKEEVYHFKDKIFYGKQAFVRAFMKSLNLNKSDLVIL
                     DRETGIGQVVFEEAQTAHLAVVVHAEHYSENATNEDYILWNNYYDYQFTNADKVDFFI
                     VSTDRQNEVLQEQFAKYTQHQPKIVTIPVGSIDSLTDSSQGRKPFSLITASRLAKEKH
                     IDWLVKAVIEAHKELPELTFDIYGSGGEDSLLREIIANHQAEDYIQLKGHAELSQIYS
                     QYEVYLTASTSEGFGLTLMEAIGSGLPLIGFDVPYGNQTFIEDGQNGYLIPSSSDHVE
                     DQIKQAYAAKICQLYQENRLEAMRAYSYQIAEGFLTKEILEKWKKTVEEVLHD"
     gene            complement(3363..5735)
                     /gene="secA2"
                     /locus_tag="VVL45_001378"
     CDS             complement(3363..5735)
                     /gene="secA2"
                     /locus_tag="VVL45_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HEU4187544.1"
                     /translation="MFRRLGQDFQLRKVKKILKQINALKGKMSSLSDQELVAKTVEFR
                     QRLSEGESLDDILVEAFAVVREADKRILGMFPYDVQVMGAIVMHYGNVAEMNTGEGKT
                     LTATMPVYLNAFSGEGVMVVTPNEYLSKRDAEEMGQVYRFLGLTIGVPFTEDPKKEMK
                     AEEKKLIYASDIIYTTNSNLGFDYLNDNLASNEEGKFLRPFNYVIIDEIDDILLDSAQ
                     TPLIIAGSPRVQSNYYAIIDTLVTTLVEGEDYIFKEEKEEVWLTTKGAKSAENFLGID
                     NLYKEEHASFARHLVYAILAHKLFTKDKDYIIRGNEMVLVDKGTGRLMEMTKLQGGLH
                     QAIEAKEHVKLSPETRAMASITYQSLFKMFNKISGMTGTGKVAEKEFIETYNMSVVRI
                     PTNRPRQRIDYPDNLYITLPEKVYASLEYIKEYHAKGNPLLVFVGSVEMSQLYSSLLF
                     REGIAHNVLNANNAAREAQIISESGQMGAVTVATSMAGRGTDIKLGKGVAELGGLIVI
                     GTERMESQRIDLQIRGRSGRQGDPGMSKFFVSLEDDVIKKFGPSWVHKKYKDYQVQDM
                     TQPEVLKGRKYRKLVEKAQHASDSAGRSARRQTLEYAESMNIQRDMIYKERNRLIDGS
                     RDLEDVVVDIIERYTEEVAADHYASRELLFHFIVTNISFHVKEVPDYIDVTDKTAVRS
                     FMKQVIDKELSEKKELLNQHDLYEQFLRLSLLKAIDDNWVEQVDYLQQLSMAIGGQSA
                     SQKNPIVEYYQEAYAGFEAMKEQIRADMVRNLLMGLVEVTPKGEIVTHFP"
     gene            complement(5728..6168)
                     /gene="asp3"
                     /locus_tag="VVL45_001379"
     CDS             complement(5728..6168)
                     /gene="asp3"
                     /locus_tag="VVL45_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HEU4187545.1"
                     /translation="MIITQRQSIHWGEVGGTYMYGSTVSYYPDKSVRLYNPLLPSGEI
                     LKTWFSSVNYQAARTQPQLPLLKRKQEYQLSLVFDCQPENGVYTKITFFDRYGDILEK
                     KVEKAKDFIFTYPEDSYTYQVSLLSAGFESLTFYHFSIKEIRSV"
     gene            complement(6165..7700)
                     /gene="asp2"
                     /locus_tag="VVL45_001380"
     CDS             complement(6165..7700)
                     /gene="asp2"
                     /locus_tag="VVL45_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HEU4187546.1"
                     /translation="MSKELNILQIGLANWENHYDIPENMSWYYFYPNSSKSLREIIEK
                     EDINRFHAVLIEDGQYSRDLFSYVKYFEPYTLFYNQNLQINDREVVDFLKKRCAQAID
                     FLSPQQLINDLSKSLFGGGYGDKLFPPTIQVNPNFTGAISYQGLDYVSLEGEFGQDFA
                     QLAYWAYNIMVQKTLPIELWLEYEKEGNCDFRLVIRKMWSGSVDDFFEEVIVSEKDLE
                     QALFMDSRDGDYFLSISVEARGRGTIKLGNLHQRWSRKQFGKFVLGGNILHDSKRDEI
                     NYFFHPGDFKPPLTVYFAGYRPAEGFEGYFMMKTLGCPFILFSDPRLEGGAFYLGTDE
                     LEGKVKDTITHYLDYLGFDHKDLILSGLSMGTFPALYYGASFEPHAIIVGKPLANLGT
                     IASRGRLDAPGVSNLAFDCLIHHTGGTSSQDMTELDQRFWKIFKQANFSKTTFGLSYM
                     KDEEMDPQAYEQLVSYLCNTGAKILSKGTAGRHNDDTDTNISWFLHFYRMVLETGFGR
                     EKR"
     gene            complement(7684..9264)
                     /gene="asp1"
                     /locus_tag="VVL45_001381"
     CDS             complement(7684..9264)
                     /gene="asp1"
                     /locus_tag="VVL45_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HEU4187547.1"
                     /translation="MYYFIPAWYGSERTWHADITPWYFSHFRLEFDDTFHQIRLFQEQ
                     DIDSRLLVLAYQPHLRYFLYRHGVLETDTYSVFDVMQDFHNLHTQVLSIRDIEWDDDC
                     EFIYSPFTIIVQKNGKKFAKVEHGVEGFISDIQYFGPNGQIHMHHIMDDRGFISSIIF
                     FEDGQAAYQEYLNPKGVWQFRERLKEGGQVEVNPILGYRFKMLTYQNMGDLVAEFFEN
                     YLQTYVKDQDIFMLPSHFHHDQLVLDRLPSTNPKLLSLFIGRNSQDTFRDLDVTFEKS
                     DLILVDREDSLRLLQELYPERMHQCYHLSSFDTRLRLGRSQTKKESIIYFQLDFEQGI
                     DNQALLQVLSFVAENKDTEVIFGAFAASQEQMNEVEGIVESFIQENIQSENLGKAIDY
                     GDAENPLEENQHQDLRIQFVNLNDELDLIKTLEFVRLIVDLNRHPHLYTQIAGISAGI
                     PQINLVETVYVEHLKNGYLLADVTEFSKAAHYYTDRLKEWNESLIYSIDKIKEHTGQQ
                     FLGKLEKWIEEVKNVKGT"
     gene            complement(9274..10491)
                     /gene="secY2"
                     /locus_tag="VVL45_001382"
     CDS             complement(9274..10491)
                     /gene="secY2"
                     /locus_tag="VVL45_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HEU4187548.1"
                     /translation="MTKIYSSIAVKKGLFTLFLLFIYVLGSRITLPFVDLNTKDFLGG
                     STAYLAFSAALTGGNLRSLSIFSVGLSPWMSAMILWQMFSYSKKLGLSSTAIEIQDRR
                     KMYLTLLIAVIQSLAVSLSLPVQSSYSVILVALMNTLLLIAGTFFLVWLSDLNASMGI
                     GGSIVILLSSIVLNIPQDVIETFKLVYIPTGIVVLLVFMTIIFSYLLALMYRARYLVS
                     VNKIGLHNRFKRYSYLEIMLNPAGGMPYMYVMSFLSVPAYLFILLGFIFPNHSGLAAL
                     SKEFMVGKPLWVYVYISVLFLFSIIFAFVTMNGEEIADRMKKSGEYIYGIYPGADTSR
                     FINRLVLRFSVIGGLFNVVMAGGPMLFVLFDEKLLRLAMIPGLFMMFGGMIFTIRDEV
                     KALRLNETYRPLI"
     gene            complement(10548..11453)
                     /locus_tag="VVL45_001383"
     CDS             complement(10548..11453)
                     /locus_tag="VVL45_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU4187549.1"
                     /translation="MSELISVVVPIYNTGKYLVECVEHILKQTYQNIEIILVDDGSTD
                     NSGEICDAFMMQDNRVRVLHQENKGGAAQAKNMGISVAKGEYITIVDSDDIVKENMIE
                     TLYQQVQEKDADVVIGNYYNYDESDGNFYFYVTGQDFCVEELAIQEIMNRQAGDWKFN
                     SSAFILPTFKLIKKELFNEVHFSNGRRFDDEATMHRFYLLASKIVFINDNLYLYRRRS
                     GSIMRTEFDLSWARDIVEVFSKKISDCVLAGLDVSVLRIRFVNLLKDYKQTLEYHQLT
                     DTEEYKDICFRLKLFFDAEQRNGKS"
     gene            complement(11453..12649)
                     /locus_tag="VVL45_001384"
     CDS             complement(11453..12649)
                     /locus_tag="VVL45_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEU4187550.1"
                     /translation="MRKSIVLAADNAYLIPLETTIKSVLYHNRDVDFYILNSDIAPEW
                     FKLLGRKMEVVNSTIRSVHIDKELFESYKTGPHINYASYFRFFATEVVESDRVLYLDS
                     DIIVTGELATLFEIDLKGYSIGAVDDVYAYEGRKSGFNTGMLLMDVAKWKEHSIVNSL
                     LELAAEQNQVVHLGDQSILNIYFEDNWLALDKTYNYMVGIDIYHLAQECERLDDNPPT
                     IVHYASHDKPWNTYSISRLRELWWVYRDLDWSEIAFQRSDLNYFERSNQSKKQVMLVT
                     WSADIKHLEYLVQRLPDWHFHLAAPCDCSEELTSLSQYTNVTVYQNVLHSRIDWLLDD
                     SIVYLDINTGGEVFNVVTRAQESGKKIFVFDITRKSMDDGLYDGIFSVERPDDLVDRM
                     KNIEIE"
     gene            complement(12658..13578)
                     /locus_tag="VVL45_001385"
     CDS             complement(12658..13578)
                     /locus_tag="VVL45_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HEU4187551.1"
                     /translation="MRNTKRAVVFAGDYAYIRQIETAMKSLCRHNSHLKIYLLNQDIP
                     QEWFSQIRIYLQEMGGDLIDLTEKEHENVEEGDQSILNMLFKDQYSSLEDQYNFQIGY
                     DYGAAAFKHQFIFDIPLEPLPLILHYISQDKPWNQFSVGRLREVWWEYSLMDWSVILN
                     EWFSKSVKYPSKSQIFKLQCVNLTNSWCVEKIDYLAEQLPEVHFHIVAYTNMANELLA
                     LTRFPNVTVYPNSLPMLLEQIVIASDLYLDLNHDRKLEDAYEFVLKYKKPMIAFDNTC
                     SENLSEISYEGIYPSSIPKKMVAAIRSYMR"
     gene            complement(13609..16053)
                     /locus_tag="VVL45_001386"
     CDS             complement(13609..16053)
                     /locus_tag="VVL45_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_1767 family glycosyltransferase"
                     /protein_id="HEU4187552.1"
                     /translation="MNKTIVLAGDRNYTRQLETTIKSILYHNRDVKIYILNQDIMPDW
                     FRKPRKIARMLGSEIIDVKLPEQTVFQDWEKQDHISSITYARYFIADYIQEDKVLYLD
                     SDLIVNTSLEKLFSICLEEKSLAAVKDTDGITFNTGVLLINNKKWRQEKLKERLIEQS
                     IITTKEVEEGRFEHFNGDQTIFNQVLQDDWLELGRAYNLQVGHDIVALYNNWQEHLAF
                     NDKPVVIHFTTYRKPWTTLTANRYRDLWWEFHDLEWSQILQHHMGEFELISPLDKEFS
                     CLTLTNSQDLEGIEELVTALPEVVFHIAAWTDMGDKLKKLAVYNNVRLHPQIVPPVLD
                     KLKKSTNLYLDINHGSADENFLKSLQEQEKTLLAFQSTQHGELGQIVFENGKVSFMID
                     TIKDFKKNGHLTCFRQLPSLTCLTFTASQYIEQLDYLAGQLPNVVFQIAAWTAMGPKL
                     YDLSNRYPNIQLYPAISRDKLDELKEKMDAYLDINLLTSTSDIVAEMAHLSKPILAFY
                     KSQNGNNGQRLYSSEHPERMLADLQKLITKDMLEKPLDIIQVKGIDETLDYIIEHNSS
                     LVRFGDGEINMLAGHSIPYQDYDEELVSIMRDIIGQESREDLVVCLPDAFTDRFRFTS
                     WAIPFWKDHMDHYMDFYRELCSDSWYGSTFVSRPYIDFEDKSQAKAQFEKLKSIWENR
                     DLLIVEGATSRSGVGNDLFDEANSIKRIICPSHSAFSRVHELEQEIEKYAGGRLILCM
                     LGPTAKVLSYNLCQMGYQVLDVGHIDSEYEWMKMGAKTKVKFSHKHTAEHNFDQDIEF
                     IDDETYNSQIVARILN"
     gene            complement(16063..17073)
                     /locus_tag="VVL45_001387"
     CDS             complement(16063..17073)
                     /locus_tag="VVL45_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU4187553.1"
                     /translation="MKLHLTNLYGMAGDSTVILAQNAVQKIASQLGFREVGIYFYNIA
                     SDSPSEMNKRLDGIMASISIGDILVFQSPTWNGFEFDRLLFDKLKDMQVKIICFIHDV
                     VPLMFDSNYYLMKDYLYMYNLSDILIVPSEQMKERLIEEGLTTKKILIQGMWDHPHDL
                     SLYTPAFKKELFFAGSLERFPDLQNWFQDTPLRVFSNKGEASSNARNLSIEGWKKDEE
                     LLLELSKGGFGLVWGTHQNEGESNQYYTLNISHKVSTYLTAGIPVIVPSSLSTAKFIV
                     DQGLGFMADSLEEVHEIVDKMNLQEYQEMTNRIKTFSYLLKEGYFTKKLLVDAIYHLG
                     ID"
     gene            complement(17102..17692)
                     /locus_tag="VVL45_001388"
     CDS             complement(17102..17692)
                     /locus_tag="VVL45_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEU4187554.1"
                     /translation="MEHDALDRLYGALKKENADISIGRYNSYDETRYVYMTYVTDPDD
                     SLEVIEGKAIMDREGVEEVRNGNWTVAVLKLFKRELLQDLPFPIGKIAEDTYWTWKVL
                     LRASRIVYLNRCVYWYRVGLSDTLSNTWSEKRMYDEIGAREEKIAILASSDYDLTNHI
                     LIYKNRLQRVIAKLEEQNMQFTEIYRRMMEKLSLLP"
     gene            complement(17707..17976)
                     /locus_tag="VVL45_001389"
     CDS             complement(17707..17976)
                     /locus_tag="VVL45_001389"
                     /inference="COORDINATES: protein motif:HMM:NF012745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU4187555.1"
                     /translation="MENELISIVVPIYNVENYLRMCLDSIQNQTYQNFECLLINDGSP
                     DHSSKICEEFVEKDSRFKYFEKANGGLSSARNLGIECSGGRTLLL"
     gene            complement(18016..18894)
                     /locus_tag="VVL45_001390"
     CDS             complement(18016..18894)
                     /locus_tag="VVL45_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HEU4187556.1"
                     /translation="MKKNHLVGDALILTVSDQIEELDYLLENLPNICFHIAAPVQFSE
                     KIRSLETNYNVRLRTITNEEQLNFLVDTCDFLLDINHFQEVDAIVSKFVQAGKSVFAF
                     DNTVHGNQGQEVFLSSTPDKLVSRVRDYLNEVRVGTNHQEKIIQDGTWNVFKIDDKAH
                     FIVGANVICRNFENCHVSSGKLILHDGVFINNSCSFNCMERIEIGAGTMMGEGVRFYD
                     HDHIYTAEKIEKWQWTTAPIRVGRDCWIGSNVTILKGVMIGDNTIIGAGCLIRNDIPS
                     NSVVYNNGNLFVKRRD"
     gene            complement(18891..20975)
                     /locus_tag="VVL45_001391"
     CDS             complement(18891..20975)
                     /locus_tag="VVL45_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000341103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU4187557.1"
                     /translation="MDDKITVIVPVYNVENYLRKCLDSIISQTYKNIEIVVVNDGSTD
                     ASGEICKEFSEMDHRILYIEQENAGISAARNTGLNNMSGNYVTFVDSDDWIEQDYVET
                     LYKKIVEYQADIAVGNYYSFNESEGMFYFHISGDSYYEKVYDNVSIFENLYETQEMKS
                     FALISAWGKLYKARLFEQLRFDIGKLGEYGYLNQKVYLLSEKVIYLNKGLYAYRIRKG
                     SLSRVWTEKWMHALVDAMSERITLLANMGYPLEKHLAVYRQMLEVSLANGQASGLSDT
                     ATYKEFEMKQRLLNQLSRQEESEKKAIVLAANYGYVDQVLTTIRSICYHNRSLRFYLI
                     HSDFPNEWIKQLNKRLEKFDSEIINCRVTSEQISCYKTDISYTVFLRYFIADFVQEDK
                     ALYLDCDLLVTKNLDDLFATDLQDYPLAAVRDFGGRAYFGQEIFNAGVLLVNNAFWKK
                     ENMTQKLIDLTNEWHNKVEQADQSILNMLFEHKWLELDFDYNHIVIHKQFADYQLPEG
                     QDYPAIIHYLSHRKPWKDLAAQTYREVWWYYHGLEWTELGQNHHLHPLQRSHIYPIKE
                     PFTCLIYTASDHIEQIETLVQSLPDIQFKIAARVIVSDRLAQMTIYPNVTIFNGIHYL
                     VDVDNELVETSQVLLDINHGEKTEEILDQFANLGKPILSFENTKTYEVGQEAYAVDQV
                     QAMIEKLREISK"
     gene            complement(21036..>22181)
                     /locus_tag="VVL45_001392"
     CDS             complement(21036..>22181)
                     /locus_tag="VVL45_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU4187558.1"
                     /translation="SASASTSASASASTSASASASTSASASASTSASASASTSASASA
                     STSASASASTSASASASTSASASASTSASASASTSASASASTSASASASTSASSSAST
                     SASESASTSASASASTSASASASTSASASASTSASASASTSASESASTSASASASTSA
                     SASASTSASASASTSASASASTSASASASTSASASASTSASASASTSASASASTSASA
                     SASTSASASASTSASASASTSASASASTSASASASTSASESASTSASASASTSASASA
                     STSASASASTSASASASTSASASVSTSASESASTSASASASTSVSNSANHSNSQVGNA
                     SGSTGKSQKELPNTGTESSIGSVLLGVLAAVTGIGLVAKRRKRDEEE"
BASE COUNT         6977 a         4759 c         3597 g         6848 t
ORIGIN      
        1 gcctgcggct agcttcctag tttgctcttt gattttcatt gagtataaaa aagaaaactt
       61 cagaccaatc cacaaaaggt gaatatctga agttttcttt tatttataag taatcaccat
      121 aaaggttaat agtagaacaa ataaagccaa actcaccaat aaagtgagca ccttgaccaa
      181 ggtgttgctc tgccagtagc ttggtttacc aatattacta gtggcatcca tggaaaatag
      241 ttgattttga cggggttgta tagttaccaa tacaatcaaa gcaagagata ggataatagc
      301 taaaacaatt agtatgtcaa tcatagatct acctcaaaat tcccagcaaa gtaaagagca
      361 aaccaatcag aatcatcaaa cccagtaaaa gtgaaaaagt cttactaatc ttttcccctc
      421 gggtttcttt ttccttcttg atgggttttt gtttcaactc ttccatgcgg ccttcaacgt
      481 ctttgtaaaa taaatcttcc tctgacatgt tagcctccta aaacagtttg catggtttcc
      541 tgatatctca ccaagtcaac ataatttgca tgttggcctt gtctgccaag tgcctgacgc
      601 atttgatcaa catctgaaag ggccaattta atggtctcaa ccaaagcagc aacttcacta
      661 ctttcaaata gatggtctgg agcgatataa agtctattgt gaaccgtttg attaaatcca
      721 agaatcaaga gattgtgctc aaaggcctga cgaactgcct gcaataactc attgctatag
      781 ttgatatcca agtaaatatc cgacagttga tacaactcct gaatcttctg tggactagca
      841 ttctggtaaa gaaccacatt aggatagcga agcatgtcta agagcttaga agacatctct
      901 gtcaccgctg cgatacggaa ggtaacatca ggcaaggctt ctacgattgc ttctacttgc
      961 tcaatctgat ctgaattggt caagattaag gcgtctcgtc ttaggaaatt atcacgtttg
     1021 aactggtaat gataacccaa gtgcacaaac tgatcatggt atttctcgtc agttaactct
     1081 aaagcgcgct cataagtcgc cttatttgga ataatgatct tcttagtacg cacattatca
     1141 ctttccaaaa tcaactgcat attacctgga atggcatcat aaagaggttc ctgccatacc
     1201 aagacatccg agccagattt atctggataa tgaaaggaaa ccaagaaagg agtcgctaga
     1261 gtattaaaga taagctgact ggtgtctatt tccaaatctt gcaaaaagaa ggtgataaat
     1321 tcaactttat ttgcaaagta acgcatggac tgaccaggca aagtcaataa gatatcaccc
     1381 gtcacatggt tttccagtaa aacttgttga ccattgacat cttggtaaac tgtcataatc
     1441 ggctcgctat ctgcgctata agtggtttta gcaaaacaag ttccgaaacg attatagtgg
     1501 tcaacctgac gtactcgacc ttctaggtct ttccagtcta cctgtttaac caagcgagcc
     1561 tgcattccat cagcataatg aatgacagcc ctctcctgcg tcacatcttc aatacaagca
     1621 gactgattat ctcctaaaat ttcccaaaaa tctgaaacgg gaacttggtt aaaatagaga
     1681 ggttttccat cctcgtaacc tagataatag gtaaaaggag aaagcagacc atcaggcaga
     1741 aaaccatctg catcgatgac cactccaagt tgagaaagac cagtagcgac tagactttca
     1801 tgtaaatctc gactttcctg actgtaactg tcataaagtt caatcatgga gcacctcctc
     1861 tactgttttc ttccactttt ctaaaatttc tttggtcaag aagccttctg caatttggta
     1921 agaataggca cgcatagctt ccaaacgatt ttcttgatac aattgacaaa tcttagcggc
     1981 ataagcttgc ttgatttggt cttctacatg gtcagatgaa cttggaatca aataaccatt
     2041 ttgcccatcc tctataaagg tctgattacc ataaggcaca tcaaaaccaa ttagaggtag
     2101 acctgaacca atagcttcca tcaaggtcag accaaatcct tcgctggtag aagccgttaa
     2161 gtagacctca tactggctat aaatctgcga aagttccgca tgccccttga gttggatata
     2221 gtcctctgcc tgatgatttg caataatttc tctaagcaga gaatcttctc caccactacc
     2281 atagatatca aaggttagtt ccggtaactc cttatgagct tcaatcacag ctttcacaag
     2341 ccaatcaatg tgtttttctt tggcaagacg tgaagccgta atcaatgaaa atggtttgcg
     2401 cccttgactt gaatctgtca aggaatcaat actgcctaca ggaatggtaa caatctttgg
     2461 ttgatgctga gtatatttgg caaattgctc ttgtagaact tcattttgtc tatcagtaga
     2521 cacgataaag aagtcaacct tatctgcatt ggtaaactga tagtcataat agttattcca
     2581 aaggatatag tcctcatttg tagcattttc actataatgc tccgcatgaa caactaccgc
     2641 tagatgtgct gtctgtgctt cctcaaacac aacctgtcca atacctgtct ccctatccag
     2701 aataaccaaa tcagacttgt tcaaattcaa agatttcata aaggcacgca caaaagcttg
     2761 ctttccatag aaaatcttat ccttgaaatg ataaacttct tccttccctt gattcatcaa
     2821 gatatcatag actggagtcc cgtcttcatt gtaaaaagtt cgttggtata agactgcaac
     2881 attgtccttt ggagcaaaat actcgctaca ataacgcgta taagaaaagt aatccttccg
     2941 aatcaggttt cccttaaaaa catactcggc atgttgaacc aagtccttgt cctcatccac
     3001 caaataacag gttacaaact tatcttggtc aaaaaagaag acacgtaaaa ccttgccatt
     3061 tttttctctg tgactttctt caccaccaaa gtaagccaag acatcatcca ctgtcacgct
     3121 agtaggcgca attttgatgt ctgtgaaatg attataaagc cagataacct gattatcatc
     3181 aaaaccaata ttggctgtta agtgctgaat attatcggct aaaatcatat ctgtaaagat
     3241 aaacttagag gacagattta atttccgaaa aacaccagca cgataggctt gagcgtattc
     3301 aacaccgcta ctagcccaac caattcctaa atttatattg taaattgtca tattttctcc
     3361 tcttatggaa aatgagtcac gatttcacct tttggagtga cctcaaccag ccccatcagg
     3421 agattacgca ccatatccgc acggatttgt tctttcatag cttcaaagcc cgcgtaggct
     3481 tcttgatagt actctacgat tggatttttc tgactagcgg attgaccacc gatagccatg
     3541 gatagctgtt gtagatagtc tacctgctct acccagttgt catcaatggc tttaagcagt
     3601 gaaagtcgta aaaactgttc atataagtca tgttgattaa gtaattcttt cttttcagaa
     3661 agttctttat caatcacctg cttcataaag ctacgaactg cagttttgtc agttacatct
     3721 atataatctg gaacctcttt aacatgaaaa ctaatattgg tcacaataaa gtgaaacagt
     3781 aattcacgac tagcatagtg atcagccgct acctcttctg tatatctctc aatgatatcc
     3841 acaacaacat cctctaagtc acgagaacca tctattagac gattacgctc cttgtaaatc
     3901 atatcccgtt gtatattcat actttcagca tattctagag tctgacgacg tgccgaacgg
     3961 ccagcactat cactagcatg ttgagccttt tcgactagtt tccggtattt acgacctttc
     4021 aatacttccg gttgagtcat atcttgaacc tgatagtctt tgtacttttt atgcacccaa
     4081 gatggaccaa atttcttgat aacatcatcc tctaaggata caaaaaattt actcatacca
     4141 ggatctccct gacgaccaga acggccacga atttgtaggt cgatccgctg actttccatc
     4201 cgctcagtcc caataacaat caagccccca agctctgcga ctcctttacc aagcttgata
     4261 tccgtaccac gtcctgccat agaggtagcc actgtcacag cccccatctg acctgactcg
     4321 gagataatct gagcctcacg cgccgcatta ttagcattta ggacattatg ggcaatccct
     4381 tcacgaaaca agagagacga atagagttga gacatttcaa ctgagcctac aaaaacgagt
     4441 aaaggatttc ccttagcatg gtattccttg atgtactcca aggatgcata cactttttca
     4501 ggtaaagtga tatatagatt atctggatag tcaatccgtt gtctcggacg attggttgga
     4561 atgcgtacta cagacatatt gtaagtttca ataaactctt tttccgcgac cttacctgtc
     4621 cctgtcatac cagatatctt attaaacatc ttaaaaagac tctgataggt gatcgaggcc
     4681 atagcccgcg tctcaggaga taatttgaca tgttccttgg cttcaatagc ctgatggaga
     4741 cctccttgaa gtttagtcat ttccattaga cgccctgttc ccttatcaac cagtaccatc
     4801 tcatttccac gaatgatata gtccttatct ttagtaaaga gcttatgagc tagaatcgca
     4861 taaaccaaat gacgagcaaa agacgcatgc tcttccttgt ataaattatc aatccctagg
     4921 aaattctcag cagacttggc ccccttagta gtgagccaaa cctcctcttt ctcctcttta
     4981 aagatataat cctctccttc gaccaaggtt gttacaagtg tatcaatgat cgcatagtaa
     5041 ttagactgaa cacgaggaga acccgcaata atcagaggag tttgtgcact atcaagcaag
     5101 atatcatcaa tttcatcaat aatcacatag ttaaacggtc gtaaaaactt accttcttca
     5161 ttcgaggcta ggttatcatt tagataatca aaacctaaat tactattggt tgtgtagatg
     5221 atatccgaag catagataag cttcttttct tcagctttca tttccttctt tggatcttcc
     5281 gtaaatggta caccaatggt caatcctaga aaacgataaa cttggcccat ttcctcggca
     5341 tcacgctttg ataaatactc attaggagtc acaaccatca ctccttctcc tgaaaaagcg
     5401 ttcaaataga caggcatggt agctgtcaag gtcttacctt cccccgtatt catctcagca
     5461 acatttccat agtgcatgac aatagctccc atgacttgaa catcataggg gaacatccct
     5521 aaaatccgct tatctgcttc acgcaccaca gcaaaagctt caaccaaaat atcgtctaga
     5581 ctttctccct cggaaagacg ctgacgaaac tctactgttt tagctactaa ttcttgatcc
     5641 gaaagagagg acatcttgcc tttcagggca ttaatctgct ttaaaatctt tttcactttc
     5701 ctaagctgga aatcttgacc taaacgtcta aacacttctg atctccttga tagaaaaatg
     5761 ataaaaagtt aaggactcaa atccagcact taaaagagaa acttgataag tataactatc
     5821 ttctggataa gtaaatatga aatctttcgc tttttctacc tttttttcta aaatatcacc
     5881 atagcggtca aaaaaagtta tcttggtata aactccattt tcaggctgac aatcaaaaac
     5941 cagtgatagt tgatactcct gctttctttt taataaggga agctgaggtt gggttcgtgc
     6001 agcctggtaa ttgacactag aaaaccaagt ctttagaatc tcaccagatg gcaatagagg
     6061 attatacaga cgaacacttt tgtcagggta ataagataca gttgatccat acatataagt
     6121 cccaccaact tctccccaat gaatagactg tctctgtgta ataatcatct tttctccctt
     6181 ccaaaaccag tctctaagac cattctataa aagtgcaaaa accaagaaat attggtatct
     6241 gtatcatcat tgtgtcgtcc agcagttcct ttagataaaa tcttcgcacc tgtattacac
     6301 agataagaca ctaattgttc ataggcttgt ggatccattt cttcatcttt catataggat
     6361 aaaccaaagg ttgtctttga aaaatttgct tgtttaaaaa ttttccaaaa acgctgatcc
     6421 aactccgtca tatcttgaga acttgtccca cctgtatgat gaattaaaca atcaaaagct
     6481 aagttagaga cacccggtgc gtccaaacgt ccacgactag ctatagttcc taaattagct
     6541 aagggcttac caactatgat ggcatggggt tcaaaagaag caccataata gagagccgga
     6601 aacgttccca tagaaagacc tgataaaatc aaatccttat gatcaaagcc taaataatca
     6661 agatagtgag tgatcgtatc ctttactttt ccctctagct catccgttcc gagataaaaa
     6721 gctcccccct ctaaacgtgg atcagaaaat aaaatgaagg gacatccaag agttttcatc
     6781 ataaagtaac cctcgaatcc ttctgcaggt cgataacctg caaaatagac agtcaaaggc
     6841 ggtttaaaat cacctggatg gaagaaatag tttatttcat cacgcttgga atcatgtagg
     6901 atatttccac caagtacaaa cttaccaaat tgttttcgac tccatcgttg gtgaagatta
     6961 cctagtttga tagttccacg acctcttgct tcaacagata tcgagagaaa gtagtctccg
     7021 tctcgactat ccataaaaag tgcttgctcc aagtcttttt cagatactat tacttcttca
     7081 aagaaatcat caacagaccc actccacatt ttacggatga ccaaacgaaa gtcacaattg
     7141 ccttccttct catattcaag ccacaactca atagggagtg ttttttgcac cataatatta
     7201 taagcccaat aggcaagctg ggcaaaatct tgcccaaact caccttccaa actgacataa
     7261 tccaatcctt gataagaaat agctcctgta aaatttggat tgacttgtat cgtcggagga
     7321 aagagtttat caccataccc accgccaaaa agagatttac ttaaatcatt gattaattgt
     7381 tggggactta aaaaatcaat tgcttgtgca catcgttttt ttagaaaatc cacaacctct
     7441 ctatcattta tttgtagatt ctggttatag aacaaagtat aaggttcaaa atattttaca
     7501 taggaaaata agtctctgga atactgacca tcttctatta aaactgcatg aaaacggtta
     7561 atatcttctt tttcgattat ttcacgaagg gattttgatg agtttgggta aaaataatac
     7621 caactcatat tttcaggtat gtcatagtga ttttcccaat tggcaagtcc tatctgtaaa
     7681 atattaagtt cctttgacat ttttaacctc ctctatccat ttctctaatt ttccaagaaa
     7741 ttgttgtcct gtgtgctcct taatcttatc aattgaatat atcaaggact cattccactc
     7801 cttcaaccta tctgtgtaat aatgtgcagc cttagaaaat tctgtaacat ctgctaacaa
     7861 ataaccattt tttaaatgtt caacatagac ggtttcaact aggttgattt gaggaattcc
     7921 tgcactaatc ccagcaatct gtgtgtagag atgaggatgt ctatttaaat ccacaatcaa
     7981 acggacaaat tctagtgttt ttatcaaatc taactcatca ttcaagttga caaactgtat
     8041 acgtaagtcc tggtgttgat tttcttccag gggattttct gcatcaccat aatcaatcgc
     8101 ctttcccaga ttttcggatt gaatgttttc ttggatgaaa gactcaacaa tcccttcaac
     8161 ctcattcatt tgctcctgac tagcagcaaa ggctccaaaa atcacctcag tatccttatt
     8221 ttcagcgaca aaggacaaga cttgaagcag agcttgatta tcaatcccct gctcaaaatc
     8281 cagttgaaaa taaatgatgg attctttctt agtttggctt cgtcccaatc gtaatcgggt
     8341 gtcaaaagat gataaatgat aacattgatg cattcgttca ggatacaact cctgcaacaa
     8401 tcgtaaacta tcctctctat ccaccaaaat caaatccgat ttttcaaaag ttacatctaa
     8461 atccctaaag gtatcttgag aattacgtcc aatgaacaga ctcaacagtt taggattagt
     8521 actaggtaaa cgatctagta ccaactggtc atgatgaaaa tgagaaggaa gcataaaaat
     8581 atcctgatcc ttcacatacg tttgcagata attctcaaaa aattctgcca ccagatctcc
     8641 catattttga taggtaagca ttttaaagcg ataacccaaa attggattga cttctacctg
     8701 tcctccttct tttaaacgct ctctaaattg ccaaactccc ttaggattca aatattcttg
     8761 ataggctgct tgcccatctt caaaaaaaat aatactcgat ataaaccctc gatcatccat
     8821 gatatggtgc atatgtattt gaccattcgg tccaaaatac tgtatatcac tgatgaagcc
     8881 ttcaactcca tgttcaacct tagcaaattt cttcccattt ttttgaacga taatcgtaaa
     8941 gggactataa ataaattcac agtcatcatc ccactcaata tctctaatgc ttaaaacttg
     9001 ggtatggaga ttatgaaaat cttgcataac atcaaaaacg gaataagtat ccgtttctaa
     9061 cacaccatgt ctatataaaa aataacgtag atgaggctgg taagctaata ctaatagacg
     9121 agaatctata tcttgctctt gaaagagccg aatctggtga aaggtatcat caaactctag
     9181 acgaaaatga gaaaaatacc atggagtgat atctgcatgc catgttcttt ctgacccata
     9241 ccaagctgga ataaaataat acataaaagt ctcctaaatc aaaggtctat aggtctcatt
     9301 tagccttaaa gccttgacct cgtctctaat cgtaaaaatc atgcccccga acatcataaa
     9361 taaacctgga atcattgcca atcgtaataa cttttcatca aacaaaacaa aaagcatggg
     9421 accacctgcc ataaccacat taaagagacc acctatgact gagaaacgaa ggaccaatcg
     9481 attaataaat cgactagtat ccgcacctgg ataaattcca taaatgtatt ctccagattt
     9541 tttcatacgg tctgcaatct cttctccatt catcgtaaca aaagcaaaaa tgatactaaa
     9601 taaaaataag accgaaatat aaacatagac ccacaaaggc ttcccaacca taaattcctt
     9661 tgataaagcc gctagccctg aatgattagg gaaaataaat cccaacaaga tgaacaaata
     9721 agctggtaca ctaagaaaac tcatcacata catataaggc atcccacctg caggattcaa
     9781 catgatttcg agataagaat agcgtttaaa tcgattgtgt aagccaattt tattaacaga
     9841 aaccaaatag cgagctcgat acataagggc aagcaagtaa gaaaatataa tggtcataaa
     9901 aacaagcaag acaacaatcc ctgtagggat ataaaccagt ttaaaagttt caataacatc
     9961 ctgagggata tttaaaacaa tgctggatag gaggattaca atagaacccc caattcccat
    10021 gctcgcattt aaatctgaca accaaacaag aaaaaacgtt cctgctatga gcaacaaggt
    10081 attcattaga gcaaccaaaa ttaccgagta ggaggattgt actggcaagc tcaagctaac
    10141 tgccaaggat tgaatcacag caattagcaa ggtcagatac atttttcgac gatcttgaat
    10201 ttcaatcgct gtagaactta gccccaattt tttagaatat gaaaacatct gccacaaaat
    10261 catagctgac atccaagggg ataatccaac agaaaaaatt gacaaacttc ttagatttcc
    10321 acctgttagg gcggctgaga aggctagata ggctgttgaa cctcctaaaa aatctttagt
    10381 atttaggtca acaaaaggga gagtaatacg acttcccaat acatagataa acagtagaaa
    10441 taaggtaaat agtccttttt ttactgctat tgacgaataa atcttagtca taaaacactc
    10501 ccctcacctt cattgtttgt gatatggtaa ataacaattc ttttatttca acttttacca
    10561 tttctttgtt ctgcatcaaa aaacaacttt aatctgaaac aaatatcttt atattcctca
    10621 gtatctgtta attgatggta ttctaaagtt tgcttataat cttttaaaag attgacaaat
    10681 cgaatacgca gaacggagac atccaaacca gccaagacac aatccgatat tttcttagaa
    10741 aacacttcaa caatatctct tgcccaggaa agatcaaatt ccgttctcat gatgcttcct
    10801 gaacgtcttc tatacagata gagattatcg tttataaaga cgattttaga ggctaaaaga
    10861 taaaagcgat gcatagttgc ttcatcatca aagcggcgac catttgaaaa gtgaacttca
    10921 ttgaataatt cttttttaat caacttaaat gtcggcaata taaaggccga gctattgaat
    10981 ttccaatctc ctgcttgacg gttcataatt tcttgtatag ctaattcttc gacgcaaaaa
    11041 tcttgccctg ttacataaaa ataaaaattc ccgtcacttt cgtcataatt atagtaattc
    11101 cctataacaa catctgcatc cttttcttga acttgctgat aaagagtttc aatcatattt
    11161 tcttttacga tatcatctga atcaacaatc gtgatgtact ctcccttagc tacactaatc
    11221 cccatatttt tagcttgtgc tgcccccccc ttattttctt gatgcaatac tcgcacacga
    11281 ttatcttgca tcataaaagc atcacaaatt tccccagaat tatccgtaga accgtcatca
    11341 actaaaataa tttctatatt ttgataggtt tgcttcagaa tatgctcgac acactccact
    11401 aaatattttc ccgtattgta tatcggtacc acaacactaa ttaattcact cattactcta
    11461 tctctatatt cttcattcta tccactaaat catctggtct ctccacagaa aaaataccgt
    11521 catagagtcc atcatccata cttttacgtg tgatatcaaa aacgaagatt ttcttgccac
    11581 tttcttgtgc ccttgtaact acattaaaaa cctctccacc tgtattaata tctaaataaa
    11641 ctatagaatc gtccaatagc caatcaattc tactatgtaa tacattttga tatactgtta
    11701 cattcgtata ctgtgataga gaggtcagct cctcagaaca atcacacggt gcagccaaat
    11761 gaaaatgcca atcaggtaac cgttgtacta aatactctaa atgttttata tctgcactcc
    11821 atgtcacaag catcacttgt tttttagact gattgcttct ttcaaaataa tttaaatcgg
    11881 aacgttgaaa agcaatctct gaccaatcca aatctctata aacccaccat aattcacgta
    11941 gtctagatat actatatgta ttccaaggtt tatcatgact agcatagtga acaattgtag
    12001 gtggattgtc atctagacgt tcacattctt gagcaaggtg ataaatatca atacccacca
    12061 tataattata tgttttatct aaggctagcc aattatcctc aaaataaata tttaaaatac
    12121 tctgatcccc aagatgaaca acttgattct gctcggccgc taattccaat aaactattga
    12181 caatagaatg ttctttccac tttgcaacat ccattagtaa cataccagta ttaaatccag
    12241 attttcgtcc ttcataggca tagacatcat caacagcacc aattgaatat cctttgagat
    12301 ctatctcaaa caaagtagct agttccccag ttacaatgat atcggaatcc agatacaata
    12361 ccctatcaga ttcaaccact tctgtcgcaa aaaatctaaa gtaagaagca taatttatat
    12421 gaggtcctgt tttatagctt tcaaaaagtt ctttatcaat gtgtacactg cgaattgtag
    12481 aattcacaac ttccattttt ctccccaata atttaaacca ttcaggagct atatcactgt
    12541 tgagaatata aaaatcaaca tctctattgt gatacaatac agactttata gtcgtctcta
    12601 aaggaataag ataggcatta tctgccgcta atactattga ttttctcata ctgttctcta
    12661 cctcatgtaa gatctgattg ctgcaaccat ttttttcgga atggagcttg gatagatacc
    12721 ttcatatgaa atctcagaaa gattttcaga gcaagtattg tcgaaagcta tcattggttt
    12781 tttgtactta agcacaaact catatgcatc ttctaatttt cgatcatgat tcaaatccaa
    12841 atacaaatct gaagctatta ctatttgttc caataacatt ggtaaggaat ttggatatac
    12901 ggtaacatta ggaaaacgcg ttaaagctag tagttcattt gccatatttg tataagcaac
    12961 aatatgaaaa tgaacttctg gcaattgctc tgccaaataa tcgattttct cgacacacca
    13021 agaattcgtt aaattaacac attgcaactt aaatatttgt gatttactag ggtacttcac
    13081 actctttgaa aaccattcat ttaaaataac agaccaatcc atcaaagagt attcccacca
    13141 aacttctctt agacgtccaa cagaaaattg attccaaggc ttatcctgag aaatatagtg
    13201 taaaattagt ggcagtggtt cgagcggaat atcaaaaatg aattgatgtt taaaggctgc
    13261 cgccccataa tcatatccta tttgaaaatt atattgatct tcaagggaac tatattgatc
    13321 tttaaacaac atattcaaaa ttgactggtc tccttcttcc acattctcat gttctttttc
    13381 tgttaagtca atcaagtcgc cccccatctc ttgtaaatat attcttattt gactaaacca
    13441 ttcctgagga atgtcctgat ttagcagata aattttcaaa tgactattgt gtctacagag
    13501 tgacttcatc gccgtttcga tttgtcgaat ataagcgtaa tcacctgcaa atactacagc
    13561 gcgtttggta tttctcattt taaatcctta tcatttattt taaatagtct agtttaatat
    13621 tcgtgcaaca atctgactgt tataggtttc atcatcaata aattcaatat cttggtcgaa
    13681 attatgttct gcagtatgtt tatgagaaaa tttaacctta gttttagctc ccattttcat
    13741 ccattcatac tctgagtcaa tatggcctac atccaaaact tgatagccca tctggcatag
    13801 attataactc agaacttttg ctgtaggtcc aagcatacat aaaatcaagc gaccaccagc
    13861 atacttttca atttcttgtt caagttcatg aactctagaa aaggcactat gagaaggaca
    13921 gataattcgc ttaatagaat ttgcctcatc gaataaatca tttccgacac ctgaacgaga
    13981 agtcgcacct tcgactatca gtaagtcacg gttttcccaa atgcttttca atttttcaaa
    14041 ttgagcttta gcttgactct tgtcttcaaa atcgatataa gggcgagata caaaggttga
    14101 gccataccat gaatcactgc ataactctct gtaaaaatcc atataatgat ccatgtgatc
    14161 tttccaaaat ggaatcgccc acgatgtaaa cctaaaacga tctgtaaaag catcaggaag
    14221 gcacactact aaatcttctc gactttcttg gccgataatg tccctcatga ttgaaaccaa
    14281 ctcttcatca taatcctggt agggaattga atgccctgca agcatattga tttccccatc
    14341 tccaaaacga actaaagaag agttgtgttc aataatataa tccaaggttt catctatccc
    14401 tttcacctgg attatatcaa gcggtttttc tagcatatcc ttagttatca atttttgcaa
    14461 atcagccaac attcgttcag gatgttcact tgaatacaac ctttggccat tattcccatt
    14521 ttgagattta taaaaggcta gtataggttt agatagatga gccatttctg caacgatatc
    14581 ggatgttgaa gtcagtaggt tgatatctaa ataagcatcc atcttctcct tcaactcgtc
    14641 tagcttatct ctagaaattg ccggatagag ctgaatatta ggataacgat tagacaaatc
    14701 atataatttt ggccccatag ctgtccaagc agcaatttga aaaacaacat ttggcaactg
    14761 tccagccaag taatccaatt gttcgatata ctgagaagcc gtaaacgtta aacaagttaa
    14821 acttggaagt tgtcgaaaac aggtaagatg tccgtttttc ttaaaatctt taatcgtatc
    14881 aatcataaag gaaactttcc cattttcgaa aacgatttgt cctaactctc cgtgctgagt
    14941 cgattgaaaa gctagtagcg ttttttcttg ttcttgcaaa gattttaaaa agttctcatc
    15001 tgcactacca tgattgatat ccaaatatag atttgttgac tttttcagct tatctaagac
    15061 cggtggaaca atttgtggat gcaatctcac attattatat acagctaatt tttttaattt
    15121 atctcccata tccgtccaag ctgcgatatg aaataccacc tcaggtagag ctgtaactag
    15181 ctcttctatt ccttctaaat cttgggaatt cgttaaggtt aagcaagaaa attccttatc
    15241 tagaggcgat attagttcaa attctcccat atggtgttgt aaaatctgac tccactccaa
    15301 atcatggaat tcccaccata aatcacgata acgattggct gtcaaggtag tccagggttt
    15361 tctgtaggtc gtaaaatgaa tcaccactgg tttatcatta aaagccagat gttcctgcca
    15421 attgttatac aaagccacaa tatcatgccc tacttgtaaa ttataagctc gacctagttc
    15481 taaccaatca tcttgcaaga cctgattgaa aattgtttga tcaccattaa aatgctcaaa
    15541 gcggccttct tcaacttcct ttgttgttat tatagactgt tcaattagtc gttcttttaa
    15601 tttctcttga cgccattttt tattgttgat taatagaaca cctgtattaa atgtaattcc
    15661 atctgtatct ttaactgctg cgagtgattt ttcttctaaa caaatactaa ataatttctc
    15721 taaagaagta tttacaatca aatcactgtc taaatataaa accttatctt cttggatata
    15781 atctgcaata aaatatctag cataggtaat gctactaatg tggtcttgct tttcccaatc
    15841 ttgaaacaca gtttgttcag gtagcttcac atcgataatc tcactaccta acatgcgagc
    15901 tatttttcgt ggctttcgaa accaatctgg catgatatct tgatttaaaa tataaatctt
    15961 aacatctcga ttgtggtata aaatagattt tatggttgtt tctaactgcc tggtgtaatt
    16021 gcgatcccct gctagtacaa ttgttttgtt catttcattc ccttaatcaa ttcccaagtg
    16081 atagattgca tctaccaata actttttagt gaaatacccc tcttttaaca aatagctaaa
    16141 ggtcttgata cgattcgtca tttcttgata ttcttgtaga ttcattttat caactatctc
    16201 atgaacctct tccagactat ccgccataaa gcccaagcct tgatctacta taaatttagc
    16261 agttgacaag ctacttggta caatgactgg aatgcccgct gtcaaatagg tactaacctt
    16321 atgagatata ttcaaggtat agtattggtt actttctccc tcattttgat gggttcccca
    16381 gacaagacca aagccacctt ttgataattc tagcaacagt tcctcatctt ttttccatcc
    16441 ttcaatgcta agatttctag cattggaact agcttctcct ttatttgaaa acaccctcaa
    16501 aggcgtatct tgaaaccaat tttgtaagtc tggaaaacgc tctaaacttc cagcaaaaaa
    16561 aagttctttt ttaaaagcag gggtgtataa ggataaatca tgaggatgat cccacatccc
    16621 ttgaatgaga atcttctttg ttgtcaagcc ttcttctatc aggcgttctt tcatctgttc
    16681 agatggaaca atcaaaatat ccgatagatt atacatatac agatagtctt tcatgagata
    16741 gtaattacta tcaaacatga gaggaacaac atcatggata aaacaaataa ttttcacctg
    16801 catatccttc agcttatcaa acaagagacg atcaaactca aagccattcc aagttggaga
    16861 ctgaaaaact aggatatctc caatagagat actggccata atgccatcca gacgcttatt
    16921 catttcagaa ggactatctg aagcaatgtt gtaaaaataa ataccaacct ctctaaatcc
    16981 cagttgacta gctatctttt gaacagcatt ttgagctaag ataaccgtac tatcaccagc
    17041 catgccgtat aaatttgtta aatgtagttt catattacgc tatctcattt tttattacta
    17101 tctacggaag taaagacaat ttttccatca ttcttctgta aatctctgtg aactgcatat
    17161 tttgttcttc taattttgct atcactcttt gtaatctatt tttataaatc aaaatatgat
    17221 tggtcaagtc atagtctgaa cttgctaaaa tagctatctt ttcttcccta gccccaattt
    17281 catcatacat acgcttttca ctccatgtat tcgataaagt atcagataaa ccaacacggt
    17341 accagtaaac acaacgattc aaatagacta tcctcgaagc tcttagaagt accttccatg
    17401 tccagtaagt atcctctgca atttttccta taggaaatgg taaatcttgt agtaactctc
    17461 tcttgaataa cttcaagaca gctacagtcc agttcccatt tctgacttct tcgacacctt
    17521 ccctatccat aattgcttta ccttctatca cttctagaga atcatctgga tccgtaacat
    17581 aagtcatata cacatagcgt gtttcatcat aagaattata acgcccgata ctaatatctg
    17641 cgttttcctt tttcaaagca ccatataatc ggtctaaagc atcatgttcc aaccaatcat
    17701 cagagtctac aaaagtaatg tacgcccccc cgaacattca atacctaggt tacgagctga
    17761 tgaaagaccg ccgtttgctt tctcaaaata tttgaaacga gaatctttct ctacaaattc
    17821 ttcacatatt ttggatgaat gatctggaga gccatcattg attaataaac actcaaaatt
    17881 ttgatacgtc tgattctgaa tgctatccaa acacattcgc aaataattct caacgttgta
    17941 gattgggact acaatactaa ttagttcatt ttccatatac taactttcct agacttgatt
    18001 tattctattt ccctcttaat ctcttctctt tacaaaaaga tttccattat tataaactac
    18061 actgtttgat ggaatatcat tgcgtatgag acaacctgct ccaataatgg tattatcccc
    18121 aatcatcact ccttttaaaa tcgtgacatt acttcctatc cagcagtctc tgccaactct
    18181 aatcggcgct gtagtccact gccacttttc aatcttttca gcagtataaa tatggtcgtg
    18241 atcataaaaa cgaacaccct ctcccatcat ggtaccagct ccgatttcaa ttctttccat
    18301 acagttaaaa ctacaagagt tgttgataaa aacaccatca tgcagtatta acttaccaga
    18361 agagacatga caattttcaa aatttctgca gatgacattt gctcctacaa tgaagtgagc
    18421 cttatcatcg attttaaaga cattccaagt cccatcttgg atgatttttt cttgatgatt
    18481 ggtgcctact ctgacttcat tcagatagtc tctcactcta gaaaccaatt tatctggtgt
    18541 acttgataga aaaacttcct gcccctgatt tccatggact gtattgtcaa atgcaaagac
    18601 agattttcca gcctgtacaa acttggagac aatagcatct acttcttgaa agtgattgat
    18661 atctagtaaa aagtcacagg tatcgactag aaagtttagc tgttcttcat ttgtaatcgt
    18721 tcggagacgg acattatagt tcgtttctaa gctacggatt ttctcggaaa actggacagg
    18781 cgctgcaata tgaaaacaga tatttggcaa gttttcaagg agataatcca actcttcaat
    18841 ctgatcacta accgtcaaaa tcagagcgtc tcctactaaa tgatttttct tcatttgctt
    18901 atttctctca atttctcaat cattgcttga acttggtcaa cagcatatgc ctcctgacct
    18961 acttcatagg ttttagtatt ttcaaaggat aagataggct tgccaagatt agcaaattga
    19021 tcgagaattt cttctgtctt ttcaccatga ttaatatcta aaagtacttg actggtttct
    19081 accaattcat tatcgacatc taccaaatag tgaattccgt taaatatagt cacgtttgga
    19141 taaattgtca tctgagccaa tcgatcacta actattactc tagctgctat cttaaactga
    19201 atatcaggca aggattgaac caatgtctca atttgttcaa tatggtctga ggcagtatag
    19261 attagacaag tgaaaggttc ctttattgga tagatgtgag atctttgtaa tggatgtaaa
    19321 tgatggtttt gtcccaattc tgtccattca agcccatgat agtaccacca aacttcacga
    19381 taggtttggg ccgccaaatc tttccacggt ttccgatgag aaagatagtg aataatagca
    19441 ggataatcct gaccctcagg caattgataa tcagcgaact gtttatgaat gacaatatga
    19501 ttataatcaa agtccaattc caaccattta tgttcaaaaa gcatattcaa aatactttga
    19561 tcagcctgtt ccaccttatt atgccattca ttagtcaaat caattaattt ttgggtcata
    19621 ttctcttttt tccaaaaagc attgtttacc aagagaacac cggcattaaa gatttcttga
    19681 ccaaaataag ctctgccccc aaaatctcta acagcagcca aaggataatc ttgtaagtct
    19741 gtagcaaaca aatcatccag atttttcgtt acaagtaaat cacagtccaa atagagggcc
    19801 ttatcttctt gcacgaaatc agctatgaaa tagcgtaaaa aaactgtgta actaatatcc
    19861 gttttataac atgaaatttg ctcagaagtt acccgacaat taataatttc tgagtcaaac
    19921 ttctctaagc gcttatttaa ttgcttaatc cattcatttg gaaaatcgct atgaatcaaa
    19981 taaaaacgaa gcgagcgatt gtggtaacag atagacctga ttgtcgttaa aacttggtct
    20041 acatagccat agtttgctgc gaggacaatg gctttctttt cactttcctc ttgtctcgat
    20101 agctgattta aaagcctttg tttcatttca aactctttat acgttgctgt gtcagataaa
    20161 ccactagctt gaccgttggc gagactgact tccaacatct gacgataaac tgccaagtgt
    20221 ttctctagag gataacccat attagctagt agcgtaatac gttcagacat agcatcaact
    20281 aaggcgtgca tccacttttc tgtccaaact cttgataaac taccttttct aatccgataa
    20341 gcataaagac ctttatttaa ataaattacc ttttccgata ataaatatac cttttgattg
    20401 aggtaaccat attctcctaa tttacctatg tcaaagcgca actgctcaaa caatcttgcc
    20461 ttatagagtt taccccaagc agatatcaaa gcaaaactct tcatttcttg agtttcatac
    20521 aagttctcaa agatggaaac attgtcatat accttctcat aataggagtc tcctgatata
    20581 tgaaagtaga acattccttc actttcgttg aaagaataat aattaccaac tgcaatatca
    20641 gcctgatact ctactatttt tttatataga gtttctacat aatcttgctc aatccaatca
    20701 tccgagtcca caaaggtcac ataatttccg gacatattat tcagaccggt gtttcgtgcg
    20761 gcagaaatac cagcattttc ttgttctata tagagaattc ggtgatccat ttctgaaaat
    20821 tctttacaaa tttcacctga agcatccgta gaaccatcat taacgacaac aatctcaata
    20881 tttttatatg tttgagaaat aatactatct agacatttcc tcagatagtt ttctacattg
    20941 tatactggta caatgaccgt aattttatca tccactagca attttctcct tgatttatgt
    21001 gcgcgagtta gtttagccta actttacagg ttgtcttact cttcttcatc acgtttacgg
    21061 cgtttcgcaa ccaatccaat acctgtaaca gctgctagaa ctccaagtaa cacagatcca
    21121 attgacgact cagtacctgt attaggcaat tctttttggg atttacctgt cgatccagaa
    21181 gcatttccaa cttgtgagtt cgaatggttt gctgaatttg aaactgatgt acttgctgaa
    21241 gctgacgcac tggttgatgc tgactcagaa gctgatgtgc ttactgaggc tgaggcactg
    21301 gttgacgctg aagctgaggc gctggttgat gctgaggctg aggcgctagt acttgctgaa
    21361 gccgaggcac ttgttgatgc ggaggctgat gcgctggttg atgccgattc agacgctgag
    21421 gtgcttgctg aggccgaagc actcgttgat gctgaagctg aggcactggt gcttgctgaa
    21481 gccgacgcac tcgttgatgc tgaagccgac gcactggtgc ttgctgaagc tgacgcactt
    21541 gttgatgcgg aagccgaagc actcgttgag gctgaagctg acgcactggt acttgctgaa
    21601 gccgaggcgc ttgtgcttgc tgaagccgaa gcactcgttg atgctgaagc cgaggcactc
    21661 gttgatgctg aagccgacgc actcgttgat gctgaggctg acgcactggt acttgctgaa
    21721 gccgaagcac tggttgaggc cgattcagac gctgaggtac ttgctgaagc cgacgcactc
    21781 gttgatgcgg aagctgacgc actggttgac gctgaagctg aggcgcttgt gcttgctgag
    21841 gctgacgcac tggttgatgc ggattcagat gctgatgtgc ttgctgagga tgaggcactt
    21901 gttgatgcgg aggctgaggc actggtactt gcggaagccg aggcgcttgt cgatgctgag
    21961 gccgaagcac ttgtacttgc tgaagctgag gcgcttgtgc ttgctgaagc cgacgcactg
    22021 gtacttgctg aagctgaggc gcttgtgctt gctgaagccg acgcactcgt tgatgcggaa
    22081 gctgacgcac tggttgatgc tgaggctgag gcgctggtgc ttgctgaagc cgaagcactg
    22141 gtacttgcgg aagccgacgc actcgttgat gctgaagctg a
//
LOCUS       DAVHEX010000039        21452 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVHEX010000039 DAVHEX010000000
VERSION     DAVHEX010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21452
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.039"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(278..1198)
                     /gene="cysK"
                     /locus_tag="VVL45_001393"
     CDS             complement(278..1198)
                     /gene="cysK"
                     /locus_tag="VVL45_001393"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEU4187559.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKPGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(1447..2082)
                     /locus_tag="VVL45_001394"
     CDS             complement(1447..2082)
                     /locus_tag="VVL45_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEU4187560.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHGLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            2138..3436
                     /locus_tag="VVL45_001395"
     CDS             2138..3436
                     /locus_tag="VVL45_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU4187561.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQVVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPML
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLAEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            3433..4095
                     /locus_tag="VVL45_001396"
     CDS             3433..4095
                     /locus_tag="VVL45_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEU4187562.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQLWCKEGVEVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            4175..4723
                     /gene="raiA"
                     /locus_tag="VVL45_001397"
     CDS             4175..4723
                     /gene="raiA"
                     /locus_tag="VVL45_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEU4187563.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNIVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            4859..6832
                     /locus_tag="VVL45_001398"
     CDS             4859..6832
                     /locus_tag="VVL45_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEU4187564.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTALLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDETSESDISQINSFVANFISEFSEKHMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDMKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEMKEKEKEE"
     gene            6829..7281
                     /gene="rplI"
                     /locus_tag="VVL45_001399"
     CDS             6829..7281
                     /gene="rplI"
                     /locus_tag="VVL45_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEU4187565.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            7325..8677
                     /gene="dnaB"
                     /locus_tag="VVL45_001400"
     CDS             7325..8677
                     /gene="dnaB"
                     /locus_tag="VVL45_001400"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEU4187566.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            8679..8945
                     /locus_tag="VVL45_001401"
     CDS             8679..8945
                     /locus_tag="VVL45_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187567.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            9034..10380
                     /gene="cbpD"
                     /locus_tag="VVL45_001402"
     CDS             9034..10380
                     /gene="cbpD"
                     /locus_tag="VVL45_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HEU4187568.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRV
                     IKANTMTGFIHFKDLDGGSVGNSQSSTSTGGTHYFKTKSAIKTEPLASGTVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGNSVLSSTGGTHYFKTKSA
                     IKTEPLVSATVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSHSSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGLWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      10463..10565
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            10636..10926
                     /locus_tag="VVL45_001403"
     CDS             10636..10926
                     /locus_tag="VVL45_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HEU4187569.1"
                     /translation="MKASIALQVLPLVQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     gene            10889..11638
                     /locus_tag="VVL45_001404"
     CDS             10889..11638
                     /locus_tag="VVL45_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4187570.1"
                     /translation="MRNLRSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRAKPW
                     QILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQYDTM
                     FAIIILVSIISLLGMKLVDISEKYVIKWKRS"
     gene            11677..12684
                     /locus_tag="VVL45_001405"
     CDS             11677..12684
                     /locus_tag="VVL45_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU4187571.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            12684..13412
                     /locus_tag="VVL45_001406"
     CDS             12684..13412
                     /locus_tag="VVL45_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4187572.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            13531..13989
                     /locus_tag="VVL45_001407"
     CDS             13531..13989
                     /locus_tag="VVL45_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEU4187573.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQIIQEVDRKGK"
     gene            13991..16423
                     /locus_tag="VVL45_001408"
     CDS             13991..16423
                     /locus_tag="VVL45_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU4187574.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVLYAILHDSNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKAKHVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLTE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            17065..17718
                     /gene="rr06"
                     /locus_tag="VVL45_001409"
     CDS             17065..17718
                     /gene="rr06"
                     /locus_tag="VVL45_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HEU4187575.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDLWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            17715..19046
                     /gene="hk06"
                     /locus_tag="VVL45_001410"
     CDS             17715..19046
                     /gene="hk06"
                     /locus_tag="VVL45_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HEU4187576.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGKQINGMYEHLLKVIYE
                     LESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIAKSI
                     NKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIENQLT
                     DATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQELEH
                     LFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            19129..19656
                     /locus_tag="VVL45_001411"
     CDS             19129..19656
                     /locus_tag="VVL45_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEU4187577.1"
                     /translation="MVLAIILVTFFIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     MAHIVFYLSCLVEAMVHKTIFDGMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYVTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            19735..>21452
                     /gene="cbpA"
                     /locus_tag="VVL45_001412"
     CDS             19735..>21452
                     /gene="cbpA"
                     /locus_tag="VVL45_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpA"
                     /protein_id="HEU4187578.1"
                     /translation="MFASKSERKVHYSIRKFSVGVASVVVASLVMGSVVHATENEGAT
                     QVPTSSNRANESQAEQGEQPKKLDSERDKARKEVEEYVKKIVGESYAKSTKKRHTITV
                     ALVNELNNIKNEYLNKIVESTSESQLQILMMESRSKVDEAVSKFEKDSSSSSSSDSST
                     KPEASDTAKPNKPTEPGEKVAEAKKKVEEAEKKAKDQKEEDRRNYPTITYKTLELEIA
                     ESDVEVKKAELELVKVKANEPRDEQKIKQAEAEVESKQAEATRLKKIKTDREEAEEEA
                     KRRADAKEQGKPKGRAKRGVPGELATPDKKENDAKSSDSSVGEETLPSPSLKPEKKVA
                     EAEKKVEEAKKKAEDQKEEDRRNYPTNTYKTLELEIAESDVEVKKAELELVKEEAKEP
                     RNEEKVKQAKAEVESKKAEATRLEKIKTDRKKAEEEAKRKAAEEDKVKEKPAEQPQPA
                     PAPKTEKPAPAPKPENPAEQPKAEKPADQQAEEDYARRSEEEYNRLTQQQPPKTEKPA
                     QPSTPKTGWKQENGMWYFYNTDGSMATGWLQNNGSWYYLNSNGAMATGWLQNNGSWYY
                     LNANGS"
BASE COUNT         6781 a         3549 c         4885 g         6237 t
ORIGIN      
        1 gggaaagctg agaaacaagg gattacgctt taccatcatg ttctaatcga tttgttcata
       61 tcttatttca atccattata aatagtgaga aatatctatc ctatcttcta gaatgtcttc
      121 caaacgtgga aactctcgta aacaaagagg ttttagaggc ctatttacca tggactaaag
      181 ttgtacaaga aaagtgcaaa taagaaatct ccagattagg aactatccgt gagttctcta
      241 gtctggagat ttttcaatag acttcgttat tggacggtta caattcataa agtgctgtag
      301 agagataacg ttcaccgtta tctggtgcta gggcaaggac ttttttacct gtacctaatt
      361 ttttggcaac ctcgatggct ccgtagatag ctgcagctga ggaaatccct acaaggaagc
      421 cttcttttcc accaatttca cgtccgagtg caagagcgtc atctgatgtt acacgaacga
      481 taccatcata ggctttagta tcaagtgtat caggaataaa tccagctgag ataccttgaa
      541 ttttgtgagg accaggtttt tcaccagata gaatagcaga ttcatctgct tctactgcaa
      601 aaacttgaat gttagaattt tctgatttga gtgcatgaga aacaccagaa atcgttccac
      661 cagtacctac tccagcaaca aaggcatcta atccatcttt accgaaagca gctagtatct
      721 cagctcctgt tgttctttcg tgtacttctg gattagctgg attgtcaaat tgaagaggaa
      781 ggaaaccatc acgttcagca gcgatttctt gagccttagc aatagcacct ttcattccct
      841 cgctaccagg agttaggacg agttcagcac cataagcttg gataatttta cgtcgttcta
      901 cactcatagt ttcaggcata acgatgacga ctttataccc tttagcagca cctacccatg
      961 aaagtccaat accggtgttt ccacttgttg cttcaacaat agtagaacca ggtttcagaa
     1021 taccatcttg ttcagctttt tcaatcatgc taagggcaat acggtctttt acagatgaac
     1081 caggattaaa tgcttcaagc tttatataga cgtctgcagc accttctggc acgatgttgt
     1141 taagtttaac aatcggtgtt tgaccgatta attcagtaat gttgttataa atagacatat
     1201 gaatacctct ttgtataaga tatctttagt ataacgctat ccataccatt tctatatatc
     1261 atagatcaaa gtcattaatt ttcaagtgat tgcgttcagc taattctagt aaataggtgt
     1321 gaaggaaaac gatatcaggt ttcgtttgaa ttgcttcatt ttctagtata ttttggtacg
     1381 cctcttttta ttttgctcgc taaagtagtt gtgagaatgt caacgaaacg ctgtattatt
     1441 cattgtttac actaagttta caggaacttc aacctctcgt aaaccttggt cagttaaagt
     1501 gacttttcca ttaaaaaact ccacaagtgc agctttaata gtttcttttt cttctttatc
     1561 aacataaatc atcgtatcga cttgatctgt aaagtttgta tccagctcca tgagaccatg
     1621 ttctttaagg aagttactgt actcttggta ctgagcataa gacatttgaa tagcaatgcc
     1681 agcctgttct tttatttcaa taataccaat ttctttgaca gctaaggcga cactgccggc
     1741 gtaagcacga attagtcctc cagcgcctag tttaatacca ccaaagtagc gtgtcacgac
     1801 cacacagaca ttggtgagat tgtgattttc tagtacccca agcatgggaa caccagcagt
     1861 accactaggc tcaccatcat cacttgtacg tttaatttca ctacgttctc caataatgaa
     1921 ggcagagcag ttatgtgtcg ctttgtagtg ttcttttttg atggcagtaa tgaagtcacg
     1981 agcctcttct tcgctataaa cacgcttggc atggcagata aagcgagatt ttttgatttc
     2041 ttcttggact tgaccgtcct ctttaattgt cctaaattcc atgatttaat tgtactaaaa
     2101 aatcatctaa agtgctagtt tttacgaata aagaagtatg aaagtaaatt tagattatct
     2161 cggtcgttta tttactgaga atgaattaac agaagaagaa cgtcagttgg cggagaaact
     2221 tccagcaatg agaaaggaga aggggaaact tttctgtcaa cgctgtaata gtactattct
     2281 agaagaatgg tatttgccca tcggtgctta ctattgtcga gagtgcttgc tgatgaagcg
     2341 agtcagaagt gatcaaactt tatactattt tccgcaggag gattttccaa agcaagatgt
     2401 tctcaaatgg cgcggccaat taactccttt tcaagagaag gtgtcagagg gattgcttca
     2461 agtagtagac aagcaaaagc caaccttagt tcatgcggta acaggagctg gaaagacaga
     2521 aatgatttat caagtagtgg ctaaagtgat caatgcgggt ggtgcagtgt gtttggctag
     2581 tcctcgcata gatgtttgtt tggagctgta caagcgcctg caacaggatt tttcttgcgg
     2641 gatagctttg ctacatggag aatcggaacc ttattttcga acaccactag ttgttgcaac
     2701 aacccatcag ttattgaagt tttatcaagc ttttgatttg ctgatagtgg atgaagtaga
     2761 tgcttttcct tatgttgata atcccatgct ttaccacgct gtcaagaata gtgtaaagga
     2821 gaatggattg agaatctttt taacagcgac ttcgaccaat gagttagata aaaaggtccg
     2881 tttaggagaa ctaaaaagac tgaatttacc gagacggttt catggaaatc cgttgattat
     2941 tccaaaacca atttggttat cggattttaa tcgctactta gacaagaatc gtttgtcacc
     3001 aaagttaaag tcctatattg agaagcagag aaagacagct tatccgttac tcatttttgc
     3061 ttcagaaatt aagaaagggg agcagttagc agaaatctta caggagcaat ttccaaatga
     3121 gaaaattggc tttgtatctt ctgtaacaga ggatcgatta gagcaagtac aagcttttcg
     3181 agatggagaa ctgacaatac ttatcagtac gacaatcttg gagcgcggag ttaccttccc
     3241 ttgtgtggat gttttcgtag tagaggccaa tcatcgtttg tttaccaagt ctagtttgat
     3301 tcagattggt ggacgagttg gacgaagcat ggatagaccg acaggagatt tgcttttctt
     3361 ccatgatggg ttaaatgctt caatcaagaa ggcgattaag gaaattcaga tgatgaataa
     3421 ggaggctggt ctatgaagtg cttgttatgt gggcagacta tgaagactgt tttaactttt
     3481 agtagtctct tacttctgag gaatgatgac tcttgtcttt gttcagactg tgattctact
     3541 tttgaaagaa ttggggaaga gaactgtcca aattgtatga aaacagagtt gtcaacaaag
     3601 tgtcaagatt gtcaactttg gtgtaaagag ggagttgaag tcagtcatag agcgattttt
     3661 acttacaatc aagctatgaa ggattttttc agtcggtata agtttgatgg agacttcctg
     3721 ttaagaaaag ttttcgcttc atttttaagt gaggagttga aaaagtacaa agagtatcaa
     3781 tttgttgtaa ttcccctaag tcctgataga tatgctaata gaggatttaa tcaggttgag
     3841 ggcttggtag aggcagcagg ctttgagtat ctggatttat tagagaaaag agaagagaga
     3901 gccagttctt ctaaaaatcg ttcagagcgc ttggggacag aacttccttt ctttattaaa
     3961 agtggagtca ctattcctaa aaaaatccta cttatagatg atatctatac tacaggagca
     4021 actataaatc gtgttaagaa actgttggaa gaagctggtg ctaaggatgt aaaaacattt
     4081 tcccttgtaa gatgaggaaa aaatttgcaa aaatagaatg aaagcgttat aataaaatca
     4141 taaaaacaaa aatgtttaga aagaaggtac tcatatgatt aaatatagta tccgtggtga
     4201 aaacctagaa gtaacagaag caattcgtga ttatgtagtt tctaaactcg aaaagatcga
     4261 aaagtacttc caaccagaac aagagttgga tgcccgaatt aacttaaaag tttatcgtga
     4321 aaaaacggct aaagtggaag taacgattcc gcttggatct attactctcc gcgcagaaga
     4381 tgtatctcaa gatatgtatg gttcaattga ccttgtaact gataaaattg aacgtcagat
     4441 tcgtaaaaat aaaacaaaaa tcgagcgtaa aaataaaaat aaggtagcaa ctggtcaatt
     4501 atttacagat gctttggtgg aagattcaaa tattgtccag tctaaagttg ttcgttcaaa
     4561 acaaattgat ttaaaaccaa tggatttgga agaagcaatt ctacaaatgg atttattggg
     4621 gcatgatttc tttatctatg tggatgttga agatcagaca accaatgtga tttatcgtcg
     4681 tgaggatggc gaaattggtt tgttagaggt taaagaatct taatttcaat atgtgtaaag
     4741 gtaggtttac tgaattgtaa actacctttt tcttataatt gatagaatta ctgattacac
     4801 ttttaaaaag tcgctttttg aggggtatta tggtataatg ggtgccaaga ggttttgaat
     4861 gaaaaaattt tatgtaagtc caatttttcc tattctagta ggattgattg cgtttggagt
     4921 cttatccact ttcattattt ttgttaataa taatctgttg acggttttaa ttttgtttct
     4981 ttttgtagga ggctatgttt ttttatttaa gaaactgaga gtgcattata caaggagtga
     5041 tgtagaacag atacagtatg taaaccacca agcggaagaa agtttgacag ctctattgga
     5101 acagatgcct gtaggtgtta tgaaattgaa tttatcttct ggagaggttg agtggtttaa
     5161 tccctatgct gaattgattt tgaccaagga agatggtgat tttgatttag aagctgttca
     5221 aacgattatc aaggcttcag taggaaatcc gtctacttat gccaagcttg gtgagaagcg
     5281 ttatgctgtt catatggatg cttcttccgg tgttttgtat tttgtagatg tatccaggga
     5341 acaagccata acagatgaat tggtaacaag tagaccagtg attgggattg tctctgtgga
     5401 taattatgat gatttggagg atgaaacttc tgagtcagat attagtcaaa tcaatagttt
     5461 tgtagctaat tttatatcag agttttcaga aaaacacatg atgttttctc gtcgggtaag
     5521 tatggatcga ttttatctat ttactgacta cacggtgctt gagggcttga tgaatgataa
     5581 attttctgtt attgatgctt tcagagaaga gtcgaaacag agacagttgc ccttgacctt
     5641 aagtatggga ttttcttatg gcgatggaaa tcatgatgag atagggaaag ttgctttgct
     5701 caatttgaac ttggctgaag tacgtggtgg cgaccaggtg gttgttaagg aaaacgacga
     5761 aacgaaaaat ccagtttatt ttggtggtgg gtctgctgct tcaatcaagc gtacacggac
     5821 tcgtacgcgc gctatgatga cagctatttc agataagatt cggagtgtag atcaggtttt
     5881 tgtagtcggt cacaaaaatt tagacatgga tgctttgggc tctgctgtag gtatgcagtt
     5941 gttcgccagc aatgtgattg aaaatagcta tgctctttat gatgaagaac aaatgtctcc
     6001 agatattgaa cgagctgttt cattcataga aaaagaagga gttacgaagt tgttgtctgt
     6061 taaggatgca atggggatgg tgaccaatcg ttctttgttg attcttgtag accattcaaa
     6121 gacagcctta acattatcaa aagaatttta tgatttattt acccaaacca ttgttattga
     6181 ccaccataga agggatcagg attttccaga taatgcggtt attacttata tcgaaagtgg
     6241 tgcaagtagt gccagtgagt tggtaacgga attgattcag ttccagaatt ctaagaaaaa
     6301 tcgtttgagt cgtatgcaag caagtgtctt gatggctggt atgatgttgg atactaaaaa
     6361 tttcacctcg cgagtaacta gtcggacatt tgatgttgct agctatctca gaacgcgcgg
     6421 aagtgatagt attgctatcc aggaaatcgc tgcgacagat tttgaagaat atcgtgaggt
     6481 caatgaactt attttacagg ggcgtaaatt aggttcagat gtactaatag cagaggctaa
     6541 ggacatgaaa tgctatgata cagttgttat tagtaaggca gcagatgcca tgttagccat
     6601 gtcaggtatt gaagcgagtt ttgttcttgc gaagaataca caaggattta tctctatctc
     6661 agctcgaagt cgtagtaaac tgaatgtaca acggattatg gaagagttag gcggtggagg
     6721 ccactttaat ttggcagcag ctcaaattaa agatgtaacc ttgtcagaag caggtgaaaa
     6781 actgacagaa attgtattaa atgaaatgaa ggaaaaggag aaagaagaat gaaagtaatc
     6841 tttttagcag atgttaaagg aaaaggtaaa aaaggcgaaa ttaaggaagt accaacaggg
     6901 tatgcgcaaa actttcttat caaaaagaat ctagccaaag aagcgactgc tcaagctgta
     6961 ggtgaacttc gtggtaaaca aaaatcggaa gaaaaagctc acgctgagat gattgcagaa
     7021 ggaaaagcaa ttaaagcaca acttgaagca gaagaaactg ttgtagaatt tgttgaaaaa
     7081 gttggtccag atggtcgtac ctttggttct attaccaata agaagattgc agaagaattg
     7141 caaaagcaat ttggaattaa gattgataaa cgtcatattc aagtacaagc tccgattcga
     7201 gcggttggtt tgattgatgt gccagtgaaa atctatcaag atatcacaag tgtaatcaat
     7261 cttcgtgtga aagaaggata agtttacacc ttcttgacaa gattgtaaaa ggaagggaag
     7321 tctgatggca gaagtagaag agttacgagt acaacctcaa gatatcttag ctgagcaatc
     7381 cgttttaggg gctatcttta ttgatgagag taaacttgtt tttgtgcgag aatacattga
     7441 gtctcgggac ttttttaagt atgcccatcg tttgattttc caagccatgg tcgatttatc
     7501 cgatcgtggt gatgccatag atgcaacaac ggttcgtact atccttgata atcaaggtga
     7561 tttacagaat attggtggct tgtcttactt ggttgagatt gttaattctg tgccaacttc
     7621 tgctaatgcg gagtattatg ctaagattgt tgcagaaaaa gcaatgctac gtcgtttaat
     7681 tgccaagttg acagagtctg tcaaccaagc ttacgaagcg tcacaaccag ctgatgaaat
     7741 tattgctcag gcagaaaaag ggttgattga tgtcagtgaa aatgcaaatc gaagcgggtt
     7801 taagaacatt cgagatgtgt tgaatctcaa ctttggaaat ctggaagctc gctcgcaaca
     7861 aacgaccgat attacaggta ttgcgacagg ttatcgtgat ttggatcata tgacaacagg
     7921 acttcatgag gaggagttga ttatcttagc agctcgtcca gcagttggta agacagcatt
     7981 tgccttgaat atcgctcaga acattgggac taagttggac aaaacggttg ctattttttc
     8041 actcgaaatg ggtgcggaaa gcttggtaga tcgtatgtta gctgcagaag gcttagtgga
     8101 gtcacattct atccgtacag ggcaattgac agatgaggag tggcaaaaat atactattgc
     8161 tcagggtaat ctagctaacg ccagtatcta tatcgatgat acgccaggta ttcggattac
     8221 agagattcgt tctcgttctc gtaaattggc tcaagaaact ggaaatcttg gtttgatttt
     8281 gatagactat ttgcaactta tcacgggaac tggtcgagaa aatcgtcaac aagaagtttc
     8341 tgaaatttct cgtcagttga aaatactagc caaggaattg aaggttccag taatcgctct
     8401 gagtcagctt tctcgtggtg tagaacaacg tcaggacaag agaccggtct tgtctgatat
     8461 tcgtgaatct gggtctattg agcaggacgc tgatatcgta gcttttctct atcgcgatga
     8521 ctactatgaa cgtggtggtg aagaagagga gggtatccca aataataagg tggaagttat
     8581 tatcgagaaa aaccgtagtg gagctcgtgg aacagtggaa ttgattttcc aaaaagaata
     8641 caataaattt tcaagtatct caaagaggga ggcataagat gtcagatgca tttacagatg
     8701 tagccaagat gaaaaaaatc aaagaagaaa tcaaggcaca tgagggacaa gtcgtagaaa
     8761 tgactttgga gaatggtcgt aagcgccaaa aaaatagatt gggtaagcta attgaagttt
     8821 atccatctct atttattgtg gagtttgggg atgtggaagg agataaacaa gttaatgttt
     8881 acgttgaatc ctttacttac tcagatattc ttacagaaaa gaatttgatt cattatcttg
     8941 actaaagtga gaaattttct cactttttct tttttctccg aataatttag gtgaaggcaa
     9001 tcatcgcttt atattatttt tcaaggagga agaatgaaaa ttttaccgtt tatagcaaga
     9061 ggaacaagtt attacttgaa gatgtcagtt aaaaagcttg ttcctttttt agtagtagga
     9121 ttgatgctag cagctggtga tagtgtctat gcctattcca gaggaaatgg atcgattgcg
     9181 cgtggggatg attatcctgc ttattataaa aatgggagcc aggagattga tcagtggcgc
     9241 atgtattctc gtcagtgtac ttcttttgta gcctttcgtt tgagtaatgt caatggtttt
     9301 gaaattccgg cagcttatgg aaatgcgaat gaatggggac atcgtgctcg tcgggaaggt
     9361 tatcgtgtag ataatacacc gacgattggt tccattactt ggtctactgc aggaacttat
     9421 ggtcatgttg cctgggtgtc aaatgtaatg ggagatcaga ttgagattga ggaatataac
     9481 tatggttata cagaatccta taataaacga gttataaaag caaacacgat gacaggattt
     9541 attcatttta aagatttgga tggtggcagt gttgggaata gtcaatcctc aacttcaaca
     9601 ggcggaactc attattttaa gaccaagtct gctattaaaa ctgaacctct agctagcgga
     9661 actgtgattg attactatta tcctggggag aaggttcatt atgatcagat acttgaaaaa
     9721 gacggctata agtggttgag ttatactgcc tataatggaa gctatcgtta tgttcaattg
     9781 gaggctgtga ataaaaatcc tctaggtaat tctgttcttt cttcaacagg tggaactcat
     9841 tattttaaga ccaagtctgc tatcaaaact gaacccctag ttagtgcaac tgtgattgat
     9901 tactattatc ctggagagaa ggttcattat gatcaaattc tcgaaaaaga cggctacaag
     9961 tggttgagtt atacggctta taacggaagt cgtcgctata tacagctaga gggagtgact
    10021 tcttcacaaa attatcagaa tcaatcagga aacatctcta gctatggatc ccatagtagt
    10081 tcaactgtcg gttggaagaa aataaatggt agttggtatc atttcaaatc aaatggttct
    10141 aaatcaacag gatggctgaa agacggttct agctggtatt atttgaaatt atctggtgaa
    10201 atgcagacag gatggttaaa ggaaaatggt ttgtggtatt atctgggtag ttcaggggca
    10261 atgaaaacag gctggtacca ggtctctggt aagtggtatt attcttactc ttcaggcgcc
    10321 ttagctgtta atacgacggt ggatggctac agagtaaaca gtgatggaga acgagtatag
    10381 aaaattggag taggagaaat ttcctgctct tttctgtaag cagtttcctt gacatttttc
    10441 ttattttgcg ctatcatata tacatataat atatgggagt ctgtgtacag tctgagagga
    10501 agtgttaaac ttcgaccgca cctgatctgg gtaatgccag cggagggaac gatacttagt
    10561 ctaattttgc accttttcca tgtatggtaa aggtttttct ttttttaaaa aggaaaacga
    10621 gaagaggagg ttcttatgaa agcaagcatt gccttgcaag ttttacccct agtacagggg
    10681 attgatcgga tagctgttat tgatcaggtc attgcttatc tgcaaactca agaagtgacg
    10741 atggtagtga caccatttga aacggtcttg gaaggggagt ttgatgagct tatgcgcatt
    10801 ctaaaagaag cgctggaagt ggcagggcag gaggcagaca atgtctttgc caatgtcaaa
    10861 ataaatgtag gagagatttt aagtattgat gagaaacttg agaagtatac tgagacgaca
    10921 cattagtcta ttgggctttc tcggagtatt gtcaatctgg cagttagcag gttttcttaa
    10981 acttctcccc aagtttatcc tgccgacacc tcttgaaatt ctccagccct ttgttcgtga
    11041 cagagaattt ctctggcacc atagctgggc gaccttgaga gtggctttac tggggctgat
    11101 tttgggagtt ttgattgcct gtcttatggc tgtgctcatg gatagtttga cttggctcaa
    11161 tgacctgatt taccctatga tggtggtcat tcagaccatt gccatagctc ctatcctggt
    11221 cttgtggcta ggttatggga ttttgcccaa gattgtcttg attatcttaa cgacaacctt
    11281 tcccatcatc gttagtattt tggacggttt taggcattgc gacaaggata tgctgacctt
    11341 gtttagtctg atgcgggcca agccttggca aatcctgtgg cattttaaaa tcccagttag
    11401 cctgccttac ttttatgcag gtctgagggt cagtgtctcc tacgccttta tcacaactgt
    11461 ggtatctgag tggttgggag gttttgaagg tcttggtgtt tatatgattc agtctaaaaa
    11521 actgtttcag tatgatacca tgtttgccat tattattctg gtgtcgatta tcagtctttt
    11581 gggtatgaag ctggtcgata tcagtgaaaa atatgtgatt aaatggaaac gttcgtagaa
    11641 ttagaatgtt tctgaaaaag aaaagaggaa atcaaaatga agaaaacatg gaaagtgttt
    11701 ttaacgcttg taacagctct tgtagctgtt gtgcttgtgg cctgtggtca aggaactgct
    11761 tctaaagaca acaaagaggc agaacttaag aaggttgact ttatcctaga ctggacacca
    11821 aataccaacc acacagggct ttatgttgcc aaggaaaaag gttatttcaa agaagctgga
    11881 gtggatgttg atttgaaatt gccaccagaa gaaagttctt ctgacttggt tatcaacgga
    11941 aaggcaccat ttgcagtgta tttccaagac tacatggcta agaaattgga aaaaggagca
    12001 ggaatcactg ccgttgcagc tattgttgaa cacaatacat caggaatcat ctctcgtaaa
    12061 tctgataatg taagcagtcc aaaagacttg gttggtaaga aatatgggac atggaatgac
    12121 ccaactgaac ttgctatgtt gaaaaccttg gtagaatctc aaggtggaga ctttgagaag
    12181 gttgaaaaag taccaaataa cgactcaaac tcaatcacac cgattgccaa tggcgtcttt
    12241 gatactgctt ggatttacta cggttgggat ggtatccttg ctaaatctca aggtgtagat
    12301 gctaacttca tgtacttgaa agactatgtc aaggagtttg actactattc accagttatc
    12361 atcgcaaaca acgactatct gaaagataac aaagaagaag ctcgcaaagt catccaagcc
    12421 atcaaaaaag gctaccaata tgccatggaa catccagaag aagctgcaga tattctcatc
    12481 aagaatgcac ctgaactcaa ggaaaaacgt gactttgtca tcgaatctca aaaatacttg
    12541 tcaaaagaat acgcaagcga caaggaaaaa tggggtcaat ttgacgcagc tcgctggaat
    12601 gctttctaca aatgggataa agaaaatggt atccttaaag aagacttgac agacaaaggc
    12661 ttcaccaacg aatttgtgaa ataatgacag aaattagact agagcacgtc agttatgcct
    12721 atggtcagga gaggatttta gaggatatca acctacaggt gacttcaggc gaagtggttt
    12781 ccatcctagg cccaagtggt gttggaaaga ccaccctctt taatctaatc gctgggattt
    12841 tagaagttca gtcagggaga attgtccttg atggtgaaga aaatcccaag gggcgcgtga
    12901 gttatatgtt gcaaaaggat ctgctcttgg agcacaagac ggtgcttgga aatatcattc
    12961 tgcccctctt gattcaaaag gtggataagg cagaagctat ttcccgagcg gataaaattc
    13021 ttgcgacctt ccagctgaca gctgtaagag acaagtatcc tcatgaactt agcggtggga
    13081 tgcgccagcg tgtagcctta ctccggacct acctttttgg gcacaagctc tttctcttag
    13141 atgaggcctt tagcgccttg gatgagatga caaagatgga actccacgct tggtatcttg
    13201 agattcacaa gcagttgcag ctaacaaccc tgatcatcac gcatagtatt gaggaggccc
    13261 tcaatctcag cgaccgtatc tatatcttga aaaatcgccc tgggcagatt gtttcagaaa
    13321 ttaaactaga ttggtctgaa gatgaggaca aggaagtcca aaagattgcc tacaaacgtc
    13381 aaattttggc ggaattaggc ttagataagt agaaaaatag ggagttggtg aagattatcc
    13441 tttaccagcg ccctttttct tttaaaaatg agaaaatttc ggtataatag tcaaacaagg
    13501 tcaaggttta aagagagagg tgggtttgtt atgagattta aaaatacatc ggatcatatt
    13561 gaggcctaca tcaaggcgat tttagatcaa tctggtatcg tggagttgca acggagtcag
    13621 ttggcagata cctttcaggt tgttcctagt cagattaact acgtgatcaa gacacgcttt
    13681 acggaaagta gaggctactt ggttgaaagt aagcgtggtg gcggaggcta cattcgtata
    13741 ggacggattg agttttctag tcatcatgaa atgctccggg agctgcttta ctcgattggt
    13801 gagcgagtca gtcaagaaat ttatgaggat attctccagc ttttggttga gcaggaattg
    13861 atgaccaagc aggagatgaa tttgctagaa tcagtagctt tggatcgcgt tttaggagaa
    13921 gaagctccag ttgttcgagc aaacatgcta cgtcagatca tacaagaggt agatagaaaa
    13981 gggaagtaag atgaactatt caaaagcatt gaatgaatgt atcgaaagtg cctacatggt
    14041 tgctggacat tttggagctc gttatctaga gtcgtggcac ttgttgattg ccatgtctaa
    14101 tcacagttat agtgtagcag gggcaacttt aaatgattat ccgtatgaga tggaccgttt
    14161 agaagaggtg gctttggaac tgactgaaac ggactatagc caggatgaaa cctttacgga
    14221 attgccgttc tcccgtcgtt tgcaggttct ttttgatgaa gcagagtatg tagcgtcagt
    14281 ggtccatgct aaggtactag ggacagagca cgtcctctat gcgattttgc atgatagcaa
    14341 tgccttggcg actcgtatct tggagagggc tggtttttct tatgaagaca agaaagatca
    14401 ggtcaagatt gctgctcttc gtcgaaattt agaagaacgg gcaggctgga ctcgtgaaga
    14461 tctcaaggct ttacgccaac gccatcgtac agtagctgac aagcaaaatt ctatggccaa
    14521 tatgatgggc atgccgcaga ctcctagtgg tggtctcgag gattatacgc atgatttgac
    14581 agagcaagcg cgttctggca agttagaacc agtcatcggt cgggacaagg aaatctcacg
    14641 tatgattcaa atcttgagcc ggaagactaa gaacaaccct gtcttggttg gggatgctgg
    14701 tgtcgggaaa acagctctgg cgcttggtct tgcccagcgt attgctagtg gtgacgtgcc
    14761 tgcggaaatg gctaagatgc gcgtgttaga acttgatttg atgaatgtcg ttgcagggac
    14821 acgcttccgt ggtgactttg aagaacgcat gaataatatc atcaaggata ttgaagaaga
    14881 tggccaagtc atcctcttta tcgatgaact ccacaccatc atgggttctg gtagcgggat
    14941 tgattcgact ctggatgcgg ccaatatctt gaaaccagcc ttggcgcgtg gaactttgag
    15001 aacggttggt gccactactc aggaagaata tcaaaaacat atcgaaaaag atgcggcact
    15061 ttctcgtcgt ttcgctaaag tgacgattga agaaccaagt gtggcagata gtatgactat
    15121 tttacaaggt ttgaaggcga cttatgagaa acatcaccgt gtacaaatca cagatgaagc
    15181 ggttgaaaca gcggttaaga tggctcatcg ttatttaacc agtcgtcact tgccagactc
    15241 tgctatcgat ctcttggatg aggcggcagc aacagtgcaa aataaggcaa agcatgtaaa
    15301 agcagacgat tcagatttga gtccagctga caaggccctg atggatggca agtggaaaca
    15361 ggcagcccag ctaatcgcaa aagaagagga agtacctgtc tacaaagact tggtgacaga
    15421 gtctgatatt ttgaccacct tgagtcgctt gtcaggaatc ccagttcaaa aactgactca
    15481 aacggatgct aagaagtatt taaatcttga agcagaactc cataaacggg ttatcggtca
    15541 agatcaagct gtttcaagca ttagccgtgc cattcgccgc aaccagtcag ggattcgcag
    15601 tcataagcgt ccgattggtt cctttatgtt cctagggcct acaggtgtcg ggaaaactga
    15661 attagccaag gctctggcag aagttctttt tgacgacgaa tcagccctta tccgctttga
    15721 tatgagtgag tatatggaga aatttgcagc tagtcgtctc aacggagctc ctccaggcta
    15781 tgtaggatat gaagaaggtg gggagttgac agagaaggtt cgcaataaac cctattccgt
    15841 tctcctcttt gatgaggtag agaaggccca cccagatatc tttaatgttc tcttgcaggt
    15901 tctggatgac ggtgtcttga cagatagcaa gggacgcaag gtcgattttt caaataccat
    15961 tatcattatg acatcgaatc taggtgcgac tgcccttcgt gatgataaga ctgttggttt
    16021 tggggctaag gatattcgtt ttgaccagga aaatatggaa aaacgcatgt ttgaagaact
    16081 gaaaaaagct tatagaccgg aattcatcaa ccgtattgat gagaaggtgg tcttccatag
    16141 cctatctagt gatcatatgc aggaagtggt gaagattatg gtcaagcctt tagtggcaag
    16201 tttgactgaa aaaggcattg acttgaaatt acaagcttca gctctgaaat tgttagcaaa
    16261 tcaaggatat gacccagaga tgggagctcg cccacttcgc agaaccctgc aaacagaagt
    16321 ggaggacaag ttggcagaac ttcttctcaa gggagattta gtggcaggca gcacacttaa
    16381 gattggtgtc aaagcaggcc agttaaaatt tgatattgca taaaagaata aaagtatcag
    16441 catctgacca taagtcacag tggagtgaaa ttcaatgaaa atcaaagagc aaactaggca
    16501 gctagccgca ggttgctcaa aacactggtt tgaggttgca gatagagctg acgtggtttg
    16561 aagagatttt cgaagagtat gaaactaaaa cctatagctt ctaaacgatc cgtggttttc
    16621 atcattcaac acaaaattca tatgtttatt accctccgtc gtatttgtct tagagcgtgt
    16681 gtagtagaaa aagagcagtc ttatctgaaa tttttattct ttcaaaagag acctgtttct
    16741 tttttgcatg tcaaatccgt tctagctggt atttgaaaaa tcaaactaat attcaatgaa
    16801 aatcaaagaa caaactagga agctagccgc aggttgctca aaacactgtt ttgaggttgt
    16861 agatagagct gacgtggttt gaagagattt tcgaagagta taagctgcaa gatgaatgat
    16921 tttcttgtat tgacgttgtt gttgacaaaa agtagcggat aaatgaaatc cattccatta
    16981 tcatagatga taggctggta ggaaattttc aaatagcata caggaaatag atgtatggag
    17041 ttctggtagt agaaagggag agagatgaac attttagttg cagatgacga ggaaatgatt
    17101 agagaaggaa ttgcagcatt tctgacagaa gagggttatc atgtcattat ggctaaggat
    17161 ggacaagagg tcttggaaaa atttcaagat ctccctatcc atctcatggt actggattta
    17221 atgatgccta ggaagagtgg ttttgaagtg ttaaaagaaa tcaatcaaaa gcacgatatt
    17281 cctgtcatcg tcttgagtgc tctgggagat gaaactactc agtcacaggt atttgatctc
    17341 tatgctgatg atcatgtgac aaaacctttt tctttggtac tgcttgtcaa gcgtattaag
    17401 gcgcttatca gacgttacta cgtcatagag gatctttggc gatatcagga tgtaacagtg
    17461 gattttacct cttacaaagc acattataaa aatgaagaaa ttgatctcaa accaaaggaa
    17521 ttactggtac taaagtgttt gattcagcat aaaaatcaag ttttaagtag agagcagata
    17581 ttggaagaaa tttcaaaaga tgtagctgat ttaccttgtg atagggtcgt tgatgtctat
    17641 attcgtactc ttcgcaaaaa attagcttta gattgtatcg tgactgtgaa aaatgttggg
    17701 tataagatta gcttatgata aaaaatccta aattattaac caagtctttt ttaagaagtt
    17761 ttgcaattct aggtggtgtt ggtctagtca ttcatatagc tatttatttg acctttcctt
    17821 tttattatat tcaactggag ggggaaaagt ttaatgagag cgcaagagtg tttacggagt
    17881 atttaaagac taagacatct gatgaaattc caagcttact ccagtcttat tcaaagtcct
    17941 tgaccatatc tgctcacctt aaaagagata ttgtagataa gcggctccct cttgtgcatg
    18001 acttggatat taaagatgga aagctatcaa attatatcgt gatgttagat atgtctgtta
    18061 gtacagcaga tggtaaacag gtaaccgtgc aatttgttca cggggtggat gtctacaaag
    18121 aagcaaagaa tattttgctt ttgtatctcc catatacatt tttggttaca attgcttttt
    18181 cctttgtttt ttcttatttt tatactaaac gcttgctcaa tcctcttttt tacatttcag
    18241 aagtgactag taaaatgcaa gatttggatg acaatattcg ttttgatgaa agtaggaaag
    18301 atgaagttgg tgaagttgga aaacagatta atggtatgta tgagcacttg ttgaaggtta
    18361 tttatgagtt ggaaagtcgt aatgagcaaa ttgtaaaatt gcaaaatcaa aaggtttcct
    18421 ttgtccgcgg agcatcacat gagttgaaaa cccctttagc cagtcttaga attatcctag
    18481 agaatatgca gcataatatt ggagattaca aagatcatcc aaaatatatt gcaaagagta
    18541 taaataagat tgaccagatg agccacttat tagaagaagt actggagtct tctaaattcc
    18601 aagagtggac agagtgtcgt gagaccttga ctgttaagcc agttttagta gatattttat
    18661 cacgttatca agaattagct cattcaatag gtgttacaat tgaaaatcaa ttgacagatg
    18721 ctaccagggt cgtcatgagt cttagggcat tggataaggt tttgacaaac ctgattagta
    18781 atgcaattaa atattcagat aaaaatgggc gtgtaatcat atccgagcaa gatggctatc
    18841 tctctatcaa aaatacatgt gcgcctctaa gtgaccaaga actagaacat ttatttgata
    18901 tattctatca ttctcaaatc gtgacagata aggatgaaag ttccggtttg ggtctttaca
    18961 ttgtgaataa tattttagaa agctatcaaa tggattatag ttttctccct tatgaacacg
    19021 gtatggaatt taagattagc ttgtagacag attagttttt tattaaagtt catatagggt
    19081 taacataagt gtgttattct ttgtgtagat aaaagaaagg atactaatat ggtattagcg
    19141 attattttag taacattctt tattcgattg atttttttaa agcgttcgat agagaatgag
    19201 aaacgaatcc ttagcaatgg cgggaaagaa tttggagttg agaatacaaa acgattaact
    19261 atggctcata ttgtttttta tctctcttgc ttggttgagg caatggtgca caagacaatt
    19321 tttgatggca tgggcatggt tggtttagtc ttgcttattt tttctatgct gatgttgatg
    19381 ttggtgattc acttgttggg agatatttgg acagtgaagc ttatgcttgt caataatcac
    19441 aaatatgtag atcatatctt gtttaggaca gtaaaacacc ctaattactt tttaaatatt
    19501 cttcctgagt tgattggctt gaccttgttg agtcatgctt atgtgacttt tgttttagtt
    19561 tttccagttt atgcagttat tttgtatcga cgaatagctg aagaggaaaa gctattacat
    19621 gaagttataa tcccaaatgg aagcataaag agataaatac aaaattcgat ttatatacag
    19681 ttcatattga agtgatatag taaggttaaa gaaaaaatat agaaggaaat aaacatgttt
    19741 gcatcaaaaa gcgaaagaaa agtacattat tcaattcgta aatttagtgt tggagtagct
    19801 agtgtagttg ttgccagtct tgttatggga agtgtggttc atgcgacaga gaacgaggga
    19861 gctacccaag tacccacttc ttctaatagg gcaaatgaaa gtcaggcaga acaaggagaa
    19921 caacctaaaa aactcgattc agaacgagat aaggcaagga aagaggtcga ggaatatgta
    19981 aaaaaaatag tgggtgagag ctatgcaaaa tcaactaaaa agcgacatac aattactgta
    20041 gctctagtta acgagttgaa caacattaag aacgagtatt tgaataaaat agttgaatca
    20101 acctcagaaa gccaactaca gatactgatg atggagagtc gatcaaaagt agatgaagct
    20161 gtgtctaagt ttgaaaagga ctcatcttct tcgtcaagtt cagactcttc cactaaaccg
    20221 gaagcttcag atacagcgaa gccaaacaag ccgacagaac caggagaaaa ggtagcagaa
    20281 gctaagaaga aggttgaaga agctgagaaa aaagccaagg atcaaaaaga agaagatcgt
    20341 cgtaactacc caaccattac ttacaaaacg cttgaacttg aaattgctga gtccgatgtg
    20401 gaagttaaaa aagcggagct tgaactagta aaagtgaaag ctaacgaacc tcgagacgag
    20461 caaaaaatta agcaagcaga agcggaagtt gagagtaaac aagctgaggc tacaaggtta
    20521 aaaaaaatca agacagatcg tgaagaagca gaagaagaag ctaaacgaag agcagatgct
    20581 aaagagcaag gtaaaccaaa ggggcgggca aaacgaggag ttcctggaga gctagcaaca
    20641 cctgataaaa aagaaaatga tgcgaagtct tcagattcta gcgtaggtga agaaactctt
    20701 ccaagcccat ccctgaaacc agaaaaaaag gtagcagaag ctgagaagaa ggttgaagaa
    20761 gctaagaaaa aagccgagga tcaaaaagaa gaagatcgcc gtaactaccc aaccaatact
    20821 tacaaaacgc ttgaacttga aattgctgag tccgatgtgg aagttaaaaa agcggagctt
    20881 gaactagtaa aagaggaagc taaggaacct cgaaacgagg aaaaagttaa gcaagcaaaa
    20941 gcggaagttg agagtaaaaa agctgaggct acaaggttag aaaaaatcaa gacagatcgt
    21001 aaaaaagcag aagaagaagc taaacgaaaa gcagcagaag aagataaagt taaagaaaaa
    21061 ccagctgaac aaccacaacc agcgccggct ccaaaaacag aaaaaccagc tccagctcca
    21121 aaaccagaga atccagctga acaaccaaaa gcagaaaaac cagctgatca acaagctgaa
    21181 gaagactatg ctcgtagatc agaagaagaa tataatcgct tgactcaaca gcaaccgcca
    21241 aaaactgaaa aaccagcaca accatctact ccaaaaacag gctggaaaca agaaaacggt
    21301 atgtggtact tctacaatac tgatggttca atggcgacag gatggctcca aaacaatggc
    21361 tcatggtact acctcaacag caatggcgct atggcgacag gatggctcca aaacaatggt
    21421 tcatggtact atctaaacgc taatggttca at
//
LOCUS       DAVHEX010000040        20887 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVHEX010000040 DAVHEX010000000
VERSION     DAVHEX010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20887
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.040"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            297..1160
                     /locus_tag="VVL45_001413"
     CDS             297..1160
                     /locus_tag="VVL45_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEU4187579.1"
                     /translation="MIEKMELGEFYKELRLARKLKQSDVACDGLTASQLSKFELGQSM
                     LSADKLILAIQGINVTFDEFGHKLNNYQESPHMQIGRKVVDRFAHQDIAGLEQLLEEV
                     EQEQMAETYRRLNAIVIKDAIHSLDKSYPLAEEDSEFLTTYLYAIESWTWFELYLFCN
                     TLPFLSNQDLIFLSTSLLEKSKEFKELVHNRLYMKQGLLNIISELMERKLFSYIPIFE
                     AELESMLRPYDVFEKLLWQFLKKMSIFLQTKGSNQKEIENFIQSLQVLENPQLTALFE
                     LRFQQYKELID"
     gene            1451..1684
                     /locus_tag="VVL45_001414"
     CDS             1451..1684
                     /locus_tag="VVL45_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187580.1"
                     /translation="MLNLQFAETMELTEAELQDVRGGNLVNSMGGGGRSGISGWGVPG
                     IYPGWGNQGMSPNRGAFDWTIDLADGLFGRRRR"
     gene            1706..2383
                     /locus_tag="VVL45_001415"
     CDS             1706..2383
                     /locus_tag="VVL45_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU4187581.1"
                     /translation="MKKMKEVKFHLATGLLILTYYLIFNVTSDLDFMVALSDNMYYVF
                     QVLLVLILGTIATIAFVKSEHWKECGRFQFRWSYLGVFLLSFFLLFVWANLTTYIFPR
                     TQNGSTVVEVATNLTGISYFVTRILYTSIIAPVSEEVVCRGLLMTSLSKVKRYYLDVL
                     VSAAIFGAMHVLQYGWITTDFIKYFGMGLIFCMMFRYTRSIYWAIALHASWNSFLLIV
                     TLLVFGY"
     gene            2395..3060
                     /locus_tag="VVL45_001416"
     CDS             2395..3060
                     /locus_tag="VVL45_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU4187582.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVTGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVVWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEDMIYRGLVMTALEKGKKWGLDVL
                     GSAVLFGVSHISNHGWVLTDFVFYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            3132..4358
                     /locus_tag="VVL45_001417"
     CDS             3132..4358
                     /locus_tag="VVL45_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4187583.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQVLQIFLGVLADFQHHRVLKYTVISFAKFLLYSIVSLSLSGQSFSLL
                     LVAFICLINLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTISYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPISTLFLRNQPFLNLQTGQSLALLATLEL
                     SALIVGSLVSGYLQHTISIKTALYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
     gene            complement(4755..4832)
                     /locus_tag="VVL45_001418"
                     /pseudo
     CDS             complement(4755..4832)
                     /locus_tag="VVL45_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            4903..5559
                     /locus_tag="VVL45_001419"
     CDS             4903..5559
                     /locus_tag="VVL45_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEU4187584.1"
                     /translation="MPTALGYVSIGLACGIIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGGLLPNPEIFGLDFALVGMFIGI
                     FASQFQIMQRRIPVRNLLIILAVVAVSFFLLLTVVSQSLAVLFATLLGCSMGVVLDGQ
                     "
     gene            5549..5872
                     /locus_tag="VVL45_001420"
     CDS             5549..5872
                     /locus_tag="VVL45_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEU4187585.1"
                     /translation="MVSKYLLLAVIFSGLVTWILRMIPFILVKYKGLPAIVERFLKFL
                     PVSIIFALILSSVVTGKVGSLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAI
                     LRLVF"
     gene            5976..6806
                     /locus_tag="VVL45_001421"
     CDS             5976..6806
                     /locus_tag="VVL45_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU4187586.1"
                     /translation="MKKIVKYSSLAALGLVAAGVLAACSGGAKKEGEAASKKEIIVAT
                     NGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            6960..7814
                     /locus_tag="VVL45_001422"
     CDS             6960..7814
                     /locus_tag="VVL45_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU4187587.1"
                     /translation="MKIKKWLGLVALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            7914..9287
                     /locus_tag="VVL45_001423"
     CDS             7914..9287
                     /locus_tag="VVL45_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU4187588.1"
                     /translation="MVFPSEQEQIEKFEKDHVAQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPNAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFDPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            9280..10341
                     /locus_tag="VVL45_001424"
     CDS             9280..10341
                     /locus_tag="VVL45_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEU4187589.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSELSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSNPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGASTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGVQ"
     gene            10343..11035
                     /locus_tag="VVL45_001425"
     CDS             10343..11035
                     /locus_tag="VVL45_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4187590.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(11065..11634)
                     /locus_tag="VVL45_001426"
     CDS             complement(11065..11634)
                     /locus_tag="VVL45_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEU4187591.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADGVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            11769..12383
                     /locus_tag="VVL45_001427"
     CDS             11769..12383
                     /locus_tag="VVL45_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEU4187592.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKVLLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            12385..14031
                     /locus_tag="VVL45_001428"
     CDS             12385..14031
                     /locus_tag="VVL45_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU4187593.1"
                     /translation="MKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHMGETVDK
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVDSIQAIAQGQEGLRIDTLEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQKYFEHQASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            14043..15329
                     /locus_tag="VVL45_001429"
     CDS             14043..15329
                     /locus_tag="VVL45_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4187594.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISGHPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVSITKQLFIQFVMDVF
                     HLFEHLKADDMTDIVKTIHAIQSFDELVSYIKETLISFFDQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKAFFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            15381..15914
                     /locus_tag="VVL45_001430"
     CDS             15381..15914
                     /locus_tag="VVL45_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4187595.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELT"
     gene            15992..16462
                     /gene="nrdI"
                     /locus_tag="VVL45_001431"
     CDS             15992..16462
                     /gene="nrdI"
                     /locus_tag="VVL45_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEU4187596.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(16755..18017)
                     /locus_tag="VVL45_001432"
     CDS             complement(16755..18017)
                     /locus_tag="VVL45_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEU4187597.1"
                     /translation="MSQAITLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
     gene            complement(18327..18785)
                     /locus_tag="VVL45_001433"
     CDS             complement(18327..18785)
                     /locus_tag="VVL45_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HEU4187598.1"
                     /translation="MKKQVFHDAATGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWATIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(18782..19222)
                     /locus_tag="VVL45_001434"
     CDS             complement(18782..19222)
                     /locus_tag="VVL45_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4187599.1"
                     /translation="MKVELQISETYEEEKLIVQAPQETEKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPTNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            19655..20521
                     /locus_tag="VVL45_001435"
     CDS             19655..20521
                     /locus_tag="VVL45_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU4187600.1"
                     /translation="MGTLLATRLKNRRKELKMSQRELAEGICKQGQISRLESGEFTPG
                     ADFLNALAKKLKVSMDYFFDEQVVEKVEELSEFKKLAQTFITNRNYESLKYIYELENV
                     KAHRLSLADKIYMEWIKSLIDFYFYGQKEDAIVRLEGVLSQLSVSDLNYLQVSNTLFN
                     FYYDIGDLGRFNKIREKLEYQVNHLNLSTIEELELSIKFNYNVCRYLWLQKNIEEAIT
                     KITATIKQCKAYRTNYLLADLYLLMGNVSENFSSKSSVKEYFETAYFLYKLEENMSMT
                     LKVEHYIADITE"
BASE COUNT         6136 a         3760 c         4589 g         6402 t
ORIGIN      
        1 ttgtaaccgc tccattataa aatcctttca ctcttccggt atactattag gaaaaacata
       61 actggaggat attttgagct cacccctcat cgttccagtg gctgttcccc aagctcgtgt
      121 actgcccaaa aaagttagac attctattta gaacgaggat tgagttctgt attgtacaag
      181 gctcggtcct tttagagtca gcttaaggct ggctttttca atcaccaaag tgtcagaatg
      241 ttttgacaaa actataaaaa caatgatata ctatataaaa aataggaggt ggtaggatga
      301 ttgaaaaaat ggaattgggg gaattttaca aggaattacg tttggcgaga aagctcaagc
      361 agtcagatgt ggcttgtgac gggctaacag cttctcagtt gtccaagttt gagctagggc
      421 agtccatgtt gtctgctgat aagctcatat tagccatcca agggatcaat gtgacctttg
      481 atgagtttgg gcacaagctc aacaactatc aagaatctcc acatatgcag attggtagaa
      541 aggttgtgga tcgctttgct catcaagata tagctggctt agagcaactg ttggaggaag
      601 tcgagcaaga acagatggca gagacttatc gtcgtttgaa tgctattgta atcaaagatg
      661 ccatccactc actagataaa agctacccac tagcagagga ggatagcgag tttttgacca
      721 cctatctcta tgctatcgag tcttggacct ggtttgaact ctatctcttt tgcaatactc
      781 tgcccttctt gagcaatcaa gatctgatct ttttatcaac ctccttactc gaaaaatcca
      841 aagaatttaa agagttagta cacaatcgat tgtatatgaa gcaaggactc ttaaatatca
      901 tctcagagct catggagcgc aaactttttt cttatatccc aatctttgaa gccgagctgg
      961 agagtatgct ccgtccatac gatgtttttg agaaattatt gtggcagttt ttaaagaaga
     1021 tgagcatttt ccttcaaacc aagggaagca accaaaaaga gattgaaaat tttatccaat
     1081 ctctgcaagt attagaaaat ccacaattaa cagccctctt cgaattgcgt tttcagcaat
     1141 ataaagaact tatagattag ttaaaaatgg aatgacaata acccactccg aaataagtag
     1201 gagtatcagt tggatttttc ttttcttcat ctattatatt cctccttgtt agtaataact
     1261 ttattatacc agggaaataa tcaaatctat caaaatcgca aataagaaat ttctataaga
     1321 aaaaatatca aatatgcgat tttttaaaat aagccaattt tcgtgttata ctgtacttgt
     1381 aaagcacttg aagcaaatcc taggtcgcag aagtggttta caaatgaaga taattgaagg
     1441 agtgtaaaag atgttaaatt tacaatttgc agaaacaatg gaattgacag aagctgagtt
     1501 gcaagatgtt agaggaggca acctcgtaaa ttctatggga ggtggtggaa gaagtggtat
     1561 atctggttgg ggcgtcccag gaatttaccc tggttggggc aatcaaggga tgagtcctaa
     1621 tcgtggagct tttgattgga ctatagattt agcagatgga ttgtttggcc gccgtagaag
     1681 ataggctctt ataggagata ataggatgaa gaaaatgaaa gaagtgaaat ttcacctagc
     1741 aactggcttg ttgattttga cttattacct catttttaat gtcacatcag acttagattt
     1801 tatggttgct ttgtctgata atatgtacta tgtgttccaa gttttattgg tacttattct
     1861 gggaactata gctacaattg cttttgtcaa aagtgaacat tggaaagagt gtggtcgttt
     1921 ccagtttcgt tggtcttacc tgggtgtttt ccttctttct tttttcttat tgttcgtgtg
     1981 ggcaaatcta acaacctata tctttcctcg tacccagaat ggaagtacag tcgtagaagt
     2041 agccaccaat ttaacaggaa ttagctattt tgttacgcga atactttata ctagtattat
     2101 cgcacctgta tcagaagagg ttgtttgtcg tggtcttttg atgacaagtt tatctaaagt
     2161 taagagatac tatcttgatg ttcttgtttc cgctgccata tttggcgcta tgcatgtttt
     2221 gcagtatggt tggataacga cagatttcat aaagtatttt ggaatgggct tgatattttg
     2281 tatgatgttt cgctacaccc gatccatcta ttgggctata gctctgcatg cttcatggaa
     2341 tagtttctta ctcattgtta ctttattggt atttggatat tagaaaggtg atatatgaaa
     2401 aagataatct cacaccgcta ctttattata gtttttctac tagttattgc tgaccagaag
     2461 ttcagtgttc tagttttacg cagcgacctt gttactgggc tatctgactt tgcctattat
     2521 ctgtcggata tgatgttgaa ttttcttgtg gttttatttg ctcttattgc tatgatttgg
     2581 tcggggaaat ggcagaaaat caatagtaga aagtttaaag gatcctatct tttctattcc
     2641 ttcctagctc tgcttgcttt tgttgtttgg aatttcgtta ccttttttct tttcccacct
     2701 actcgaaatg aaatttctta tcaacatgct gctccgactt ttacaggggc tacagcattt
     2761 ttgatgtatt ttttttatcc tgtgattgca ggtcccattt ttgaagacat gatttatcgt
     2821 ggattagtga tgactgctct ggaaaaagga aagaaatggg ggctagatgt acttggttcc
     2881 gctgttttat ttggggtctc gcacattagt aatcacggtt gggtcttgac agactttgtc
     2941 ttctatatgg gtggaggtct tatatttgca gtcttattta gaatgacaaa gtcaatttat
     3001 tggcctattg gactgcatat agtctacaat ggcattggtc agcttttgat gttactgtaa
     3061 ttttggttgt tagtagtatc gcggtctatc cttggggaaa gattcccatt tgaacttaag
     3121 aaggagttgt gatgaaagta tttcttcaaa atagagattt taggcaatta actatcaacc
     3181 agtggatttc aacgcttggg gatacgattt tttatctggc cttcctgaat tatgtggcag
     3241 atgcatcttt tgcccctttg gcgattttac tcatcacgat ttcagaaacc ctgccccaag
     3301 ttctacaaat ctttctggga gttttggcgg attttcaaca tcatcgtgtc ttgaaataca
     3361 cagtcattag ttttgcaaaa tttttgcttt actctatagt gtctttatca ctttcagggc
     3421 agtctttctc cttgttgcta gtagcattta tttgtctgat taacctcttg tctgacacat
     3481 tgagttattt ttcaggcgcc atgctcactc cgattttcat tagaattatt gggcaagacc
     3541 atctggcaga agctattggc tttaaacagt caactgttag tttagtgaaa acaatcagta
     3601 atatcctagg aggagtctta ctaggcattc tatccatcca gtttatttcc ttactgaacg
     3661 ctcttacctt tttaatcgca tttttaggta tcctcttcat aaaaactgac ctcttgaaag
     3721 tagaaaaaac gattagctat caagaaggac tctctgtaaa atccttttgc cagcatttgc
     3781 tccaatcatc gaaattgata tggaatatga ataaggtgct cttggttttg tttattatct
     3841 ctactagtca agcagtgata aatgttacgg ttcctatttc cactctattt ttaaggaacc
     3901 agcccttttt gaatttacaa actggtcaat ctcttgcctt gttagccaca ctagaattat
     3961 cagccctgat tgtcggaagc cttgtaagtg gctatctgca acataccatt tccataaaaa
     4021 cagctctcta tgcctcactt gtcatccagt tgcttcttct agtaggattt gccacagttc
     4081 gttttgactg gattctcatc tttagtacct tggatgcctt tttcgcaggt gttctctctc
     4141 ctagattgca agaactcgtt tttaaacaaa tacctgagga gtcaatggga gcagttcaat
     4201 cctctatcgg tgccattacg gttgttttac ctagcttatt tacaatagct ttggtaacca
     4261 ttgctactag ctttggaact ctggcagtta gctttgtttt attgctattt cttctagttg
     4321 cctttgttat gcttctgaat attcgtgaaa gtatttagtg gagaatttct ataatatatg
     4381 tttgagactg gattgccagt ctttttatga tggtcaaaat caacagactt ttttctcttt
     4441 tctgaatgtg ttataataga ctgaatctaa aatagtacga aacaattgct aaaacattta
     4501 tagaaattaa ttttactttc ccaatcgatt tgttctcatc ttatttcaat ctgctatata
     4561 atagtccatg ctgtggctgg attcttttca gcctacttgt actcttcgaa actctcttca
     4621 aaccacgtca gcttcacttt gccgtaggta tggttactga cttcgtcagt tctatccaca
     4681 acctcaaaac ggtgttttga gctgacttcg tcagttctat ccacaacctc aaaacagtgt
     4741 tttgagctga cttcatcagt tctatctgca acctcaaaac agtgttttga gcaacctgcg
     4801 gctagcttcc tagtttgctc tttgattttc attgagtatt attaagacaa tatgaggaga
     4861 aagatgaaag aaaaaggatt ttaggagggg gcgcaggcag ccatgccaac ggctcttggc
     4921 tatgtcagta tcggcctggc ctgtggaatt atcggtgcgc cctatgtgac acctgttgag
     4981 atgggcttga tgagtctctt tgtttatgct gggagtgccc agtttgccat gttggcactg
     5041 attgtggttc aagctcctgt ggcagctatt gctatgacgg tttttctaat caacttgcgt
     5101 ctctttttgt tgagtttaca cgcatcgact tatttccgtc ataccagtct ttggtacaat
     5161 atcggtatgt ctagtatctt gacagatgag acctatggcg ttttgatggg tgaattggcc
     5221 catacagaca aggtaaatcc tatgtggatg cacggaaaca atcttaacag ctatgtggct
     5281 tggtttgtgg ggacagtagt cggaacggct ctgggtggcc tgctaccaaa tccagaaatc
     5341 tttggcttgg attttgccct ggttgggatg tttattggta tttttgcttc gcaatttcag
     5401 attatgcaaa gacggattcc tgtccgcaat ctgctcatta tcctagcagt tgttgcggtg
     5461 tccttctttt tgctcttgac agtggtgtct cagtcactag ctgttctgtt tgcgacgcta
     5521 cttggttgta gcatgggggt ggttttagat ggtcagtaag tatcttttat tagcagttat
     5581 tttctctggc ttggtgactt ggattctccg tatgattccc ttcatcttag tcaagtataa
     5641 gggcttgcct gcaatcgttg agcgtttttt gaagttcttg cccgtttcca ttatctttgc
     5701 cttgattctt tcaagcgtag tgacaggtaa ggttgggagc cttcctcaaa ttaaatggct
     5761 agacttctta gccgtctttc caacagcttg ggtagccttt cgctaccgca atctagtcgg
     5821 aacagttctc tttggagtgg tcttgattgc catcttgcgt ttggtctttt aaattaccca
     5881 taaaaaaaac ttatcacaga gatagatatc atataatggc gtaaatgctc tttttctgtt
     5941 aagattataa ggtattctat tttggaggaa atgacatgaa aaaaatcgtt aaatactcat
     6001 ctcttgctgc cctaggactt gttgctgcag gtgtgcttgc ggcttgctca gggggtgcta
     6061 agaaagaagg agaagcagct agcaagaaag aaatcatcgt tgcaaccaat ggatcaccaa
     6121 ggccatttat ctatgaagaa aatggcgaat tgactggtta cgagattgaa gtcgttcgcg
     6181 ctatctttaa agattctgac aaatatgatg tcaagtttga aaagacagaa tggtcaggtg
     6241 tctttgctgg tcttgacgct gaccgttaca atatggctgt caacaatctt agctacacta
     6301 aagaacgtgc ggagaaatac ctctatgccg caccaattgc ccaaaatcct aatgtccttg
     6361 tcgtgaagaa agatgactct agtatcaagt ctctcgatga tatcggtgga aaatcgacgg
     6421 aagtcgttca agccactaca tcagctaagc agttagaagc atacaacgct gaacacacgg
     6481 acaacccaac tatccttaac tatactaagg cagatttcca acaaatcatg gtacgtttga
     6541 gcgatggaca atttgactat aagatttttg ataaaatcgg tgttgaaaca gttatcaaga
     6601 accaaggttt ggacaacttg aaagttatcg aacttccaag cgaccaacaa ccgtacgttt
     6661 acccacttct tgctcaaggt caagatgagt tgaaatcatt tgtagacaaa cgcattaaag
     6721 aactctacaa agacggaacg cttgaaaaat tgtctaaaca attcttcgga gatacttatc
     6781 taccagcaga ggctgatatt aaataatttc ttgaaatcat ccattctgag taagatgggt
     6841 gatttctcag tttttaggga tatagtttta aactatatat ctgattatct aataacaatt
     6901 tgtgaaatca aacaaattgt gagatactag tacggtatta tttttaagga gaaagaatca
     6961 tgaaaatcaa aaaatggctt ggcctagtag cccttgctac agtcgcaggt ttggctcttg
     7021 cagcttgcgg aaactcagaa aagaaagcag acaatgcaac aactatcaaa atcgcaactg
     7081 ttaaccgtag cggttctgaa gaaaaacgtt gggacaaaat ccaagaattg gttaaaaaag
     7141 acggaattac cttggaattt acagagttca cagactactc acaaccaaac aaagcaactg
     7201 ctgatggcga agtagatttg aacgctttcc aacactataa cttcttgaac aactggaaca
     7261 aagaaaacgg aaaagacctt gtagcgattg cagatactta catctctcca atccgccttt
     7321 actcaggttt gaatggaagt gccaacaagt acactaaagt agaagacatc ccagcaaacg
     7381 gagaaatcgc tgtaccgaat gacgctacaa acgaaagccg tgcgctttac ttgcttcaat
     7441 cagctggctt gattaaattg gatgtttctg gaactgctct tgcaacagtt gccaacatca
     7501 aagaaaatcc aaagaacttg aaaatcactg aattggacgc tagccaaaca gctcgttcat
     7561 tgtcatcagt tgacgctgcc gttgtaaaca ataccttcgt tacagaagca aaattggact
     7621 acaagaaagc acttttcaaa gaacaagctg atgaaaactc aaaacaatgg tacaacatca
     7681 ttgttgcgaa aaaagattgg gaaacatcac ctaaggctga tgctatcaag aaagtaatcg
     7741 cagcttacca cacagatgac gtgaaaaaag ttatcgaaga atcatcagac ggtttggatc
     7801 aaccagtttg gtaataagaa acagggaggt gggagagaga attccacctc ttgctcttgt
     7861 atagagcacg gagtgtaaag aagactattc gttcatagaa aggtagagag aatatggttt
     7921 ttcctagcga acaagaacag attgaaaaat ttgaaaagga tcatgtagcc cagcattatt
     7981 ttgaggtttt gcgtaccttg atttctaaga aatcagtctt tgcccagcag gttggactca
     8041 aggaagtcgc aaattatctg ggtgagattt tcaagcgtgt tggagctgaa gtggagattg
     8101 atgagagcta tacagcgccc tttgtcatgg cacatttcaa gagttcgcgt ccaaatgcca
     8161 agaccttgat tttctataac cactatgaca ctgtgccagc ggatggggat caggtctgga
     8221 cagaggatcc ttttacgctt tcggtccgca atggcttcat gtatgggcgt ggagttgatg
     8281 acgacaaggg tcatatcaca gctcgcttga gtgctttgag aaaatatatg cagcaccatg
     8341 atgatttacc tgtcaatatc agctttatca tggagggagc ggaggaatcg gcttcaacag
     8401 acctagataa gtatttggaa aagcatgcag acaaactccg tggggcggat ttgttggtct
     8461 gggaacaagg gaccaaaaat gccttggaac agctggaaat ttctggtggc aataagggga
     8521 ttgtgacctt tgatgccaag gtaaaaagcg ctgatgtgga tatccactcg agttatggtg
     8581 gtgttgtgga atcagctcct tggtatctcc tccaagcctt acagtctctt cgtgctgcgg
     8641 atggccgtat cttggttgaa ggcttgtacg aagaagtaca agagcccaat gaacgagaaa
     8701 tggccttgct agaaacttat ggtcaacgaa acccagagga agttagtcgg atttatggat
     8761 tggagttgcc tctcttacag gaggagcgga tggcctttct aaaacgtttc tttttcgatc
     8821 cagcgcttaa tatcgaagga atccagtctg gttatcaagg tcagggtgtt aagactattt
     8881 tacctgcaga agccagtgcc aagctagagg ttcgtctggt tccgggccta gaaccgcatg
     8941 atgttctgga aaaaattcgg aaacagctag acaaaaatgg ctttgataag gtagaattat
     9001 actatacctt gggagagatg agctatcgaa gcgatatgag cgcaccagcc attctcaatg
     9061 tgatcgagtt ggccaagaaa ttctatccac agggcgtttc agtcttgccg acgacagcgg
     9121 ggacaggacc tatgcatacg gtctttgatg ccctagaggt accaatggtt gcattcggtc
     9181 taggaaatgc caatagccga gaccacggtg gagatgaaaa tgtgcgaatc gctgattatt
     9241 acacccatat cgaattagta gaggagctga ttagaagcta tgagtagaga tattatcaag
     9301 ttagatcaga tcgatgtgac ttttcaccaa aagaagagaa ccatcacagc ggttaaggat
     9361 gtgaccattc acatccaaga aggggatatc tacggaatcg ttggatattc tggagcagga
     9421 aaatcaaccc ttgtacgggt gattaatctc ttgcaaaaac catctgcagg gaaaattacc
     9481 attgacgacg atgtgatttt tgacggcaag gtgaccttga cggcagagca gttgcgtcgt
     9541 aaacgtcaag atatcggaat gattttccag cattttaacc tgatgagcca aaagacagca
     9601 gaggagaatg tagcctttgc ccttaaacac tctgaactca gcaaggaaga aaagaaggct
     9661 aaagtagcta agttgttgga cttggttggt ttggcagatc gtgctgaaaa ctacccttca
     9721 caactatctg gagggcaaaa acagcgtgtg gcaattgcgc gtgccttggc caatgatcca
     9781 aaaatcttga tttcagacga gtcaacttct gcccttgatc cgaagacaac caagcagatt
     9841 ttggccttgt tgcaagattt gaaccaaaaa ttaggcttga ctgttgtctt gattacgcat
     9901 gaaatgcaga ttgtcaaaga cattgccaac cgtgttgcag ttatgcagga tgggcatttg
     9961 attgaagagg gtagtgtgct tgaaatcttc tcaaacccta aacaaccttt gactcaagac
    10021 tttatctcaa cagctacagg tattgacgaa gccatggtca aaatcgagaa gcaagaaatc
    10081 gtggaacact tgtctgaaaa cagtctcttg gtgcaactca agtacgctgg agcttcaaca
    10141 gacgagccac ttttgaatga attgtacaag cattaccaag taatggctaa tattctctat
    10201 gggaatatcg aaattctcga tggtactcct gttggagaat tggtggtggt tttgtcaggt
    10261 gaaaaagcag cgttggcagg tgcccaagaa gccattcgtc aagcaggtgt acaactaaaa
    10321 gtattgaagg gagtacagta agatggaatc attgattcaa acctatttac caaatgtcta
    10381 taagatgggt tgggctggtc aggcaggctg gggaacggct atctacttaa ctctttatat
    10441 gacagttctt tccttcatta tcggaggctt cttggggcta gtggcaggtc tctttctcgt
    10501 cttgacagcg ccaggtggtg tcttggagaa taaagtcgta ttctggattt tagacaaaat
    10561 tacctcaatt tttcgtgcgg ttccctttat catcctcttg gcaatcttgt caccactttc
    10621 tcacttgatt gttaaaacaa gtatcgggcc aaatgcagcc cttgtcccac tttcttttgc
    10681 agtctttgcc ttctttgccc gtcaggtgca ggttgtcttg gctgaattgg atggcggtgt
    10741 cattgaggcg gctcaagcga gcggagcgac tttctgggac atcgtgggtg tttacctatc
    10801 agaaggtctt ccagatttga tccgtgtgac gactgtgacc ttgatttccc ttgttgggga
    10861 aacagctatg gccggtgcgg ttggagctgg tggtatcggt aacgtagcca tcgcttatgg
    10921 atttaaccgc tacaatcacg atgtgaccat cttggcaacc attattatca ttttgattat
    10981 ctttgcaatc caattcttgg gagatttctt gactaagaaa ttgagccata aataaaaaag
    11041 agccgcgtgg ctctttttaa ctgatcagat tttctgggca aattttttac tcaaggcttg
    11101 tccaatcaag gcacccacta gggctccgat gacaatactt gcgataaata gaaggacagt
    11161 tccagggttt ggagcgacca tgatacggtc gatatattct tgggattttc ctcttgccag
    11221 aagagtagcc atataggctt tgggcgcaat ccacataagc aagattggtc ctgttgtact
    11281 aaaggcgaaa ataatgaaag aaaggaagtt ctttgttttg tccttgtatt ttcctaaatg
    11341 agctactcca tctgctagga ggccacagat aattccagga aggaaggaac cagcaccgtg
    11401 tttagttccc aagaaaaaga gggcaatgac aaggccgata gtggtaatgg ctccaaagcg
    11461 cggaactttt gcgactagga tcatatagac gctaccgccg acaagggcag taaaggcagg
    11521 cgcataaaac atgtttccag tttggtcaaa gagattgccc aaaaggacac caatccccat
    11581 gcagaggaag taaagaactg caaaaagcag tgtagttaag atagatttct tcatgaattg
    11641 tctcctgata attttttcac aattctcatt ttatcatggt ttggtgggat tgtaaatgga
    11701 tactagaagt ttttgaaaac gcttgaaata tgatataatg gtttcatagt tatttttagg
    11761 ggaatatcat gggtagattt ttagactttg tctttaatcg tttcttttta gggatgattg
    11821 cgacagcctt cttttggcta ttaactttag caggagggat tatccttggt ctagcgccgg
    11881 ctagtgccac cttgatgagc ttatatgcag aacatggtta tagctttcgg gaatacagtt
    11941 tgaaggaggc ttggtctctt tacaagcaaa attttgtctc aagcaacctg attttctata
    12001 gctttttagg tgtgggtcta gttttgacct atggtttgta tctcttggtg caattgcctc
    12061 atcagaccat tgttcatttg attgcgaccc ttttgaatgt cctagtagtt gccctgatct
    12121 ttttggctta tacagtatct ttaaaattac aagtttattt tgccttgtcc tatcgaaata
    12181 gtctcaaatt atccttgatt ggcatcttta tgagtctagc agctgtggct aaggttctcc
    12241 ttgggactgt gctacttgta gcaattggtt attatatgcc tgccctgcta ttttttgtag
    12301 gaattgggat gtggcatttc tttatcagtg atatgttgga acctgtctat gaaatcatcc
    12361 atgaaaaatt ggcgacaaaa tagaatgaag cagttttggc tacatacgct tctaagaacc
    12421 tatagttcag tgatgatcat tatcattgcg agttttgcaa tcttactctc ttacgctgac
    12481 tgggattcac gtgaaaagga agcccagaga gtagcccagc gtgtaactgc tagaacagtg
    12541 agtgaaattg aatattacca tagagagtca acccagatag ctcaggcttt agttgaaaat
    12601 caagctcgta tcgagggaat ctataaatac tttagcctta gcatgccaga ctatttttac
    12661 tggcaattag agcggaaagc ttcgccttat atatcagtct ctctgtatga aaatgttgat
    12721 gacctctatg ttcgaaatga ttttgtaact ggggtggcca ttgcttttca agattacaag
    12781 gaagtctatg tttctactaa agacaaacgt agtggagaaa aaatcagggc tgaggatttc
    12841 aaaccagcag gaaatagttt tgccattcca gtgtcagatc cagtgtcaga tcaagactta
    12901 ggagtgattt acatctcctt ggatcctgct gttttatacc atgccattga taatactaga
    12961 ggtcatactc cgatggcagt aacagtgacc tcaccttttg atacggagat ttttcatatg
    13021 ggtgagacag ttgataagga gagtgaaaat tggctagttg gcttaacttc tcatggttat
    13081 caggttcagg tggcagttcc caaaaacttt gttttacaag gaacggtgac cagctctgct
    13141 ttgattgtgg gcttgagcct tctctttatt gtcattcttt atctgacttt gaggcagacc
    13201 tttgctaatt atcaaaagca ggtagtggat ttggtggatt ccatccaagc tattgcccaa
    13261 ggacaagaag gtcttcgcat tgatacgctt gaaaaggatc aggaattgct cctaatcgcg
    13321 gagacgacca atgatatgtt ggatcgattg gaaaagaata tccatgatat ttaccagtta
    13381 gaactcagtc aaaaagatgc caatatgcgg gccttgcagg cgcaaatcaa ccctcacttc
    13441 atgtataata cgctggagtt cttgcgtatg tacgcagtca tgcagagtca agatgagttg
    13501 gcagatatca tttatgaatt cagtagtctc ttgcgtaaca atatttccga cgaaagagag
    13561 accctcctca aacaggaatt agaattttgc cgtaaataca gctatctctg catggttcgc
    13621 tatcccaagt ccattgccta tggtttcaag atagatccag agttagagaa tatgaagatt
    13681 cccaagttta ccttgcaacc gctggtagaa aactatttcg cgcatggtgt tgaccacagg
    13741 cggacagata atgtgattag catcaaggct cttaaacagg atgggtttgt ggaaattttg
    13801 gtggtcgata atggtagagg aatgtcggct gaaaagttgg caaatatccg agaaaaatta
    13861 agtcagaaat attttgaaca ccaagccagc tacagtgatc aaaggcagtc tatcgggatt
    13921 gtcaatgtac acgagcgttt tgtgctctat tttggagacc gctatgccat tactatagag
    13981 tctgcagagc aagccggtgt tcagtatcgt attacaattc aagatgagta gaaagggaga
    14041 aaatgtataa agtattatta gtagatgatg agtacatggt gacagaaggt ctgaagcgtt
    14101 tgattccctt tgataagtgg gatatggagg tcgtcgcaac agccagtcat gccgatgaag
    14161 ctctagaata tgttcaggaa aatcctgtcg atgtcatcat ttccgatgtc aatatgccag
    14221 acaaaacagg gcttgatatg attcgggaga tgaaagagat cttaccagat gctgcctata
    14281 tcctgctctc aggttatcag gagtttgatt atgtaaaaag agcaatgaac cttagtgtgg
    14341 tggactattt ggtcaagcct gttgataagg tagagctggg aaatctgctg gagaagattg
    14401 caggtcagct cggcgagaga gggaagaaaa gtcagactct tagtcaagaa ttagacgagg
    14461 ctggatttgt tagttattta ggggataagg agaattggtg gataggtcta tccaaggaaa
    14521 aacaaggttc cttcaccatt ccctactatg tcttgggtca agactggcag attttcattt
    14581 ctggccaccc cctagatggt ttagtcgtta caccttttga agctccttat caagaacatt
    14641 ttgaacgctg gaagctgaat gctgagaaaa ccctctttta cggttctgta aatctgcagc
    14701 agtctgagag tctctttgcc tattacgaac cgatttatag ggttatcatt cagggaaatc
    14761 tcaatcaaat cgtagaagag ttaaatctct tggagaaggt agttcttgaa aatacacctc
    14821 gtgtttcgat tactaaacag ctttttatcc agtttgtcat ggatgttttc catttatttg
    14881 aacatctcaa agctgatgat atgacggaca ttgtcaaaac cattcatgct attcaatcct
    14941 tcgatgaatt ggtttcttat atcaaggaaa ctctgatcag ctttttcgat caataccgta
    15001 tgaatgaaaa tgtggtcagt gtgctggaag tcattggtcg tgattaccaa aaagagcttt
    15061 ccctcaagga tatcagtaag gccttcttta tcaatcctgt ctatctaggg cagttgatta
    15121 agcgtgaaac cgattcgacc tttgcagagt tactaaataa acaacgtatt aaggctgccc
    15181 agcagctctt gctttcaact agtgacagca tcgaagatat ttgttatgct gttggttaca
    15241 gtaaccttgg atatttctat aaagttttcc gaaaattgtg cggaaaatcg ccaaaagcct
    15301 accgaaaaca ggtagaaact atactataag atttgtattc ctttacaaaa ggtgctataa
    15361 tattatcagg aggtcctatc atgaaaaaga aaccaattta tctatgggtt ttgctaatct
    15421 tgtctgccct tatttcagtt ccgtctctgt ttggaattgt gagtcccttg cctagtaaag
    15481 aagcccttcg tgctgctcag aaacaagttg caggggtcaa tgctcagcaa ttagaagacc
    15541 agcttaatta tacctataga gtagcagaag catctcattc tatttttaat gttgctttga
    15601 ttgtgctatc tactatttta gtggtcgtag cgattgtctt ccttgttcgt aaaaatttgc
    15661 aatatgcaaa ctatacttac gttggctatg ttttgttagc tattattggt tcgatttatg
    15721 gctatgttgg tttacaagac gctgtgcagt tggttcaaga tgagagcatg cgtttgacag
    15781 tgagtattgg ttcaaaagct gttagcattt tctatatcgt catcaatgtt ctcttcctag
    15841 cccttgtctt ctataagatg tggcgacaac agaaagcctt ggcagaagaa gaggaaacag
    15901 aagagcttac ctaagtattg acaaaaaaga actatcaaga tacaatcttg gtagttgttt
    15961 tttgattata gataaaatca tggaggtaca tatgaagaca atttctttag tttatattag
    16021 tttgagtggc aacactgaaa gttttgtgac gcgcttgaaa gactatctct tgtcccagta
    16081 caaagggatt gaggttcaaa agattcatat caaggatttg gtcaaggaag gcaaaaattt
    16141 ctatgaaatg gaccatccct atgtcgcttt tttgccgacc tatctcgaag gtgggaatgg
    16201 cgtggataac ggagatgttg agattttgac gacaccggtg ggagatttta ttgcctatgg
    16261 taacaatgct agtaagtgtt ttggtgtggt tgggtcagga aatcgtaact ttaataacca
    16321 atactgccta acagccaagc aatacagtca acgctttggc ttccctgtat tggctgactt
    16381 tgaaatgcga ggcatgctgg aagatatcaa acatgtcgca gctattatcg cagatttgta
    16441 tgagttggaa aaggagaatt aatactcaat gaaaatcaaa gagcaaacta ggaagctagt
    16501 cgcaggttgc tcaaagcaca gctttgaggt tgcagataga gctgacgtgg tttgaagaga
    16561 ttctcgaata gtataaagtt cttataagag gtggttgaag ttgttcaact gttttttgag
    16621 tataaacagt ctttaagaac gtaaaaaagc ataagttcag gtcttcaaaa ccttgttctt
    16681 atgcttttta tcatagaaat atttgctgaa ttttctcttg aaatgaaacc ttatcttcat
    16741 ctcgcgcttg tcccttaccc tgcttttccg attgcgagtg cgtagataaa gaagatggcg
    16801 aagccaaaga ggaaaatcaa tcccgcaatg gcaaggtgtt tgagccaaga aggaagattt
    16861 ttgttttcac gcgttaccaa ggcgtaattc aaaagagcaa agaaaggtgt tgtcaggaaa
    16921 gagccaatca tggcaaagcg gagcatggtt gaaacctgac cagcgaagaa cttgataatg
    16981 acgataccga tgatagcagt gatggtcatc cagatgttca aagatttacg attgtcctct
    17041 ttttgactga ttagcagtcg gagagattcc tgattaacgc gagaatagcc atcgataaca
    17101 gttataactg ttccaaagat acagaggaag gcaataaagg taatcaagta acgggaccat
    17161 tcgccaagaa cagaggcata catgcccacg aattgagaga tgtatttggc tgaagcagct
    17221 tcaaccgcct gccctgtagg atactgaatc agtgctccca gtgccacaaa gaagacggct
    17281 aggatagctg ttccaatata accagtgtta aagtcaaaca gagcgtcttc tgtgttaaag
    17341 ttgacggtct ttctcttttc agctgaccaa agtgaattga tggctgaaat ttcaatagga
    17401 gccggcatcc atcctaggag ggagacgatg aagggcagag ctgccatttg ccaaggtgtc
    17461 ttctcgacaa aatcagaact gtattctgga tgcttgaccg ccgcaatgat aactgcaaga
    17521 acagtcgcaa tggttaaggc agacataatc catttgacca tgccgtctaa aagtttgtag
    17581 cctccaaaga gtagcatagc ccaaatgatt gcaacgagaa tgagggacca ctgagtaatg
    17641 ctaagtccaa tcattgggaa agcactggcg atgatagctg agcacagaat ggcaacacca
    17701 gccgtgttga ccatagccga aaagacattg aggataaaga aaatccagag atagagtttt
    17761 cctttttcgg cataaccttc aaccaaagtc tttccagtat cagctgtgta ttcagcacca
    17821 aaacggaaaa atggatattt aaagacattg gctaagatga ccaagagaag tagagaccaa
    17881 ccgtaagaac cgccagcttg agttgaggat acaatgtggg aacctccaac agcggcagtt
    17941 gccattagga ttccaggtcc cattgctttt agcttgcttg cccaggttga ttgatttaag
    18001 gtaatagctt gcgacatgat aaatactcct ttttgaattt tcagaataat ctgtatatgt
    18061 aatctatatt ctacaaaaaa ttttggaaaa atgcaagaat attttgaaaa aagatagaaa
    18121 atttacaaag aaaatctgaa ataattgtta tgatatagca gattgaaact agaatagtac
    18181 acctttgctt ctaaaacatt gttagaaatc tatttgactg tcctgatcga tttatcctgt
    18241 tcttatttca tttcactata gtaaggtgca gcaggataaa gaaaaaccga gaagtttctt
    18301 tctcggattt gcttattatg aggttcctat tttctatgct ccaagagttg attgatgtga
    18361 tctacttttt tagcctctct tttatagaga atagcccaga tgatgagata gacaatcgca
    18421 aactcgataa tgagctggag gtagaaaatc cagtggaaag ggaaccaacc agcaagagtt
    18481 gctagtggga caaagccagc cagcataagg aagaaatgag tcagagtggc acgaagcatg
    18541 ctccagtcac ggctaaataa gcggttgcca aagttgaaga gcataccgat ggttgcccag
    18601 ataagtgtgc agtagagcaa gaccagggca ccgtgaacct gatgctgatc catcacttgg
    18661 ccgatgagag agtagggatt tagtggtgcg taggtatttg gtgcataaat gagtgaaaag
    18721 aggatagaga ggatgaggcc gataagaaca ccggtagctg catcgtgaaa tacttgtttt
    18781 ttcatagttc taatttctcc ttgatggttt ttagataacg gcgtgaagag taggtgaagc
    18841 tttcgttttt caagaaaatt tctaccagac cgtttggcgt gagcttgagg tgagagatgg
    18901 aatcgatatt gatgatttct gattgggaaa tttggataaa attggttggc aagagtttaa
    18961 gaacctgata gagtcgcaaa tcaatgctgt aggtctgact cgcggtttct gctagaacct
    19021 tccgattctc gatatagaag cgctgaatct tgccaatctc aactagatag acctgatcat
    19081 cgatttttcc tttgattttt tctctttggt ccagattttc tgcgaactcg atgactttct
    19141 ggactttttc ggtttcttga ggtgcttgga caatcagctt ttcctcctcg taagtctcac
    19201 taatctgtag ttctactttc atagttttct ctccttttca gttatacaag gttgtgatca
    19261 cttcctgtat atccttatta aacactattt tatagcccat ggcaaggaac cttgtctatg
    19321 tggagggatt gggctcctat gtggtggagc ttttctgttc tttctgaaat atggtataat
    19381 agcactaatc aatttctagg aaaatagata cggaaagggg ctgaaagaac taggggaaca
    19441 gttggaatta gaaagtgata ggtatgaaaa gtggcgttga agctgaataa gtttataaag
    19501 cgtttgaatg aagaaatgga aagtaaataa gttgacgatg aattacaaaa tagattttat
    19561 ctacaatttg tatctttcaa atcattgatt tgaattttag atttaatcat gttacaatta
    19621 aaactgaaat caaatagaag aggaaaatgc aaacgtgggc acactattag caacaagatt
    19681 aaaaaataga cggaaagaat taaaaatgtc tcagcgagaa ctagctgaag gtatctgtaa
    19741 acaggggcaa atcagtcgat tagagagcgg agagtttacc cctggagcgg atttcctgaa
    19801 tgcccttgct aagaagttaa aagtcagtat ggattatttt tttgatgagc aggttgtaga
    19861 gaaagttgag gagttatcag aatttaagaa attagcacag acatttatta caaatcgcaa
    19921 ttatgagtct ttgaaatata tatatgaatt ggagaatgta aaagctcacc gcctatcttt
    19981 agcagataaa atctacatgg aatggataaa atctctgata gatttttatt tttacggaca
    20041 gaaagaagat gcgattgtac gattagaggg ggtattatca cagttaagtg tatctgattt
    20101 gaactatctc caagtttcaa acaccctatt caatttttat tatgatattg gagatttagg
    20161 tcgtttcaat aaaattcgtg aaaaactaga gtatcaagta aatcacctca atttgagtac
    20221 aatcgaagag ttagaactat ctattaagtt taactacaac gtttgtcgtt atctatggtt
    20281 acagaaaaat attgaagaag ctattactaa aattacagct actataaaac aatgtaaggc
    20341 ttacagaacg aattatcttt tggctgattt gtacttattg atgggaaatg taagtgaaaa
    20401 tttttcttct aaaagttcgg tgaaagaata ttttgagacg gcatattttc tatacaaact
    20461 tgaggagaac atgtcaatga ctctgaaagt tgagcattac attgcagata taacagaata
    20521 atgaatacgg tgttcaagtg agcatcgttt ttcaaaccta gttcagatac aaaaaagtat
    20581 caaacaaaaa aaaataaaaa aacattataa aaacaattga caagctaatt ttgaagtgat
    20641 aaaataaatg caagaaaaca aaaggagagg tatatgagaa gtttgtttaa gaaaatagtt
    20701 gctgtattag ttattggctt aattctgtta ggaattgcga gaacacctca agttcataag
    20761 atggctagag ggattgatcc tggtcctgcg aattttattt agaaatcaat ttctactaat
    20821 tgagtattaa tgggtaaaaa gattgtatgg ataagaaaaa tttacttgta ttggttactg
    20881 gttcggt
//
LOCUS       DAVHEX010000041        20817 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVHEX010000041 DAVHEX010000000
VERSION     DAVHEX010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20817
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.041"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..427
                     /locus_tag="VVL45_001436"
     CDS             <1..427
                     /locus_tag="VVL45_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU4187601.1"
                     /translation="RVFEGYPAYGGNGIAWKSRKYLIDNPTIIIGRVGAYCGNVRTTH
                     GKVWISDNAIYIKEFKNSDFNLVFLLELMKVIDFSKFADFSGQPKITQKPLENQKYIL
                     PPLVLQNEFADFVALVDKSQFACEIAIKVWRNSLKFSII"
     gene            457..3807
                     /locus_tag="VVL45_001437"
     CDS             457..3807
                     /locus_tag="VVL45_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU4187602.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYRATLSSLVWDDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMIKLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIDQLSEAETRKLFIDIDLRLA
                     GWIFEENCRVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSNHRRQALLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDKEGAQSSRMVFSTYPTMIGA
                     ISGQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSEEEKEHFD
                     SKFEDNSCEKDIDGSVFNSFIFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHD
                     HAEYIRGIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIKEKYPQIAISVDMLDTGI
                     DVPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRA
                     DPRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLN
                     ELDFRVRMVLDTVYSYRKLESWQNLTAVTSETIQKNLSPLLFDEDKEDEMARRFDLWL
                     LHIQLGQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEIIRKVQEPEFWKEVNLS
                     DLEKIRLAIRDLLQFLDKTDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHY
                     LKTHLDEESISKLYHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREII
                     GLDRESANRIFSKFLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQ
                     LLFQHQLPVLRNIVQIIELINNRAGEAA"
     gene            complement(4313..4783)
                     /locus_tag="VVL45_001438"
     CDS             complement(4313..4783)
                     /locus_tag="VVL45_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU4187603.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAENDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(4800..7073)
                     /locus_tag="VVL45_001439"
     CDS             complement(4800..7073)
                     /locus_tag="VVL45_001439"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEU4187604.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVKLPHKIELEKPQLNLVQPQGKAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
     gene            7420..10521
                     /locus_tag="VVL45_001440"
     CDS             7420..10521
                     /locus_tag="VVL45_001440"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU4187605.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQGVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNVFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQERFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGQSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     ETEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRLAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSLEAGHTVEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNSSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIENRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSNELEEKLNEIVMKT
                     IYR"
     gene            10603..11610
                     /gene="pfkA"
                     /locus_tag="VVL45_001441"
     CDS             10603..11610
                     /gene="pfkA"
                     /locus_tag="VVL45_001441"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEU4187606.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            11669..13174
                     /gene="pyk"
                     /locus_tag="VVL45_001442"
     CDS             11669..13174
                     /gene="pyk"
                     /locus_tag="VVL45_001442"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEU4187607.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(13445..13738)
                     /locus_tag="VVL45_001443"
                     /pseudo
     CDS             complement(13445..13738)
                     /locus_tag="VVL45_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167986.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(13905..14130)
                     /locus_tag="VVL45_001444"
                     /pseudo
     CDS             complement(13905..14130)
                     /locus_tag="VVL45_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            14978..15850
                     /locus_tag="VVL45_001445"
     CDS             14978..15850
                     /locus_tag="VVL45_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HEU4187608.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(15996..16340)
                     /locus_tag="VVL45_001446"
                     /pseudo
     CDS             complement(15996..16340)
                     /locus_tag="VVL45_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            16483..16794
                     /locus_tag="VVL45_001447"
     CDS             16483..16794
                     /locus_tag="VVL45_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187609.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEHPELYAVAQTSGEIPEKKREELRQIITTIFEQKMNKHSI
                     K"
     gene            16798..17514
                     /locus_tag="VVL45_001448"
     CDS             16798..17514
                     /locus_tag="VVL45_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU4187610.1"
                     /translation="MKKYFIGGLGSNAYHSKDFLQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEESICLIGHSIGGDLARYFASEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIVSDLALLISKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNGATWREEALKELPDYFNTIFLENFGHEL
                     YTQAPKEIASLMNEWLAYFL"
     gene            complement(17644..18459)
                     /locus_tag="VVL45_001449"
     CDS             complement(17644..18459)
                     /locus_tag="VVL45_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEU4187611.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWITAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(18456..19361)
                     /locus_tag="VVL45_001450"
     CDS             complement(18456..19361)
                     /locus_tag="VVL45_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEU4187612.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGILFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMLLAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLAYSLLIGVIL"
     gene            19703..>20817
                     /locus_tag="VVL45_001451"
     CDS             19703..>20817
                     /locus_tag="VVL45_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1919 domain-containing protein"
                     /protein_id="HEU4187613.1"
                     /translation="MLHQGDKNSLLESLSIVERQTLKDIEVIIVNQSTEEIASISQQM
                     NLSFNIKIIDENSFAELSDMITGDYITFLSSNDSLFDWTFEKMYHAIKLSDSDVVLGH
                     FADLVDGVFYFYPFWGDNWLTENLTNVQVLQKMDDTDHRIRKQYRSLFGKLFNSKLLR
                     KINQFDINNLIWRLYIHSKTATYINFPTYIYKPVIDAKPLKDSYKIILENYENRIKDC
                     AVLENYDIERAKKQYIQELANFSAWLKNDGEYLDSDIVYHKLIAAEKGIFPFLELDRL
                     DFTIISNNCVGGLIYKQLGFQYRTPFVGLFILPDDYYKLTKHFRYYMEQELVFEEELI
                     SPSWTHEVYPLGHLGDIDIHFLHYSSIEEARTKWEKR"
BASE COUNT         6560 a         3497 c         4374 g         6386 t
ORIGIN      
        1 tagagttttt gaaggttatc ctgcctatgg tggaaatggc attgcttgga aaagtcgaaa
       61 atatttaatt gataatccta ctatcatcat tggtcgtgta ggtgcatatt gtggtaatgt
      121 aagaactacc cacggtaagg tatggataag tgacaatgct atatatataa aagaatttaa
      181 gaattccgat ttcaatcttg tatttttatt agaattaatg aaagtaattg attttagtaa
      241 atttgctgat ttctcaggtc aaccaaagat aactcaaaaa ccattagaaa atcagaaata
      301 tatcctcccc cctctagtcc tccaaaatga gttcgcagac tttgtagccc tggtcgacaa
      361 atcacaattt gcttgtgaga tagctataaa agtgtggaga aatagcttga aatttagtat
      421 aatatagcta aactatttgt ttaaagtgag aaaaaaatgg gaaattttag ctttctttta
      481 aaaaatgacg aatatgaatc tttttcaaaa ccttgcattg aagctgagaa tatgattgct
      541 acatcaactg tggctactgc ctttatggcg cgtcgtgctt tagagcaggc tgtccattgg
      601 atatatagtc acgattcata tttagaagct ccctatcgtg ctactctatc ttctttagta
      661 tgggatgatg attttaggga tatcgtagat tctgaactcc acaagcagat agttctgttg
      721 attcggtggg gaaaccatgc tgctcatggt ggtgaaatta aggaacgaga agcgatttta
      781 gctttgcatc atttgtatca gtttgttaat tttatcgatt attgttacag caatgagttt
      841 gtggagcgtt attttgatga gaagtgctta ccactttcag caaacatcaa ataccgagaa
      901 actccacaat ctatgataaa gttacaagac agtttaccag aactgcctga ttttcatgaa
      961 cagatggctg ctcagtccgt agaagttcaa gagacttata ctgaaaaacg tgagactgca
     1021 gcgcaacggc aagatgtgcc tttccatatt gatcaattat ctgaggcaga gacaagaaag
     1081 ctctttattg atatcgatct ccgtttagca ggatggatat ttgaagaaaa ctgtcgtgtt
     1141 gagatagccg ttgatggtct caagcacggt tcaggaattg gttactgtga ctatgtactt
     1201 tatggtaaaa atgggaaaat tttagcgatt gtagaggcta aaaaagcctc tgtcaatcca
     1261 gaagtagggg aagtacaggt caaagaatat gctgaagctc ttgagaaaca tatcggctat
     1321 cagccaattt gctttattac aaatggcttg aagcactata tacttgatgg tccgaaccgc
     1381 cgccagattg caggctttta ctctcaagaa gaattgcaat tagtgatgga tagacgtcat
     1441 cttcaaaaac cacttgagga tatttctagt aaaattaggg acgatatttc cgggcgtcac
     1501 taccaaaaac atgccattgc aagcgtttgt gaagctttct ctaatcatcg tagacaggca
     1561 cttttggtta tggcaactgg ggctgggaaa actcgtacag cagtttctct agttgatatc
     1621 ttatcacgtc ataactgggt aaaaaacgtt ctcttcttag ccgatagaac ttccttggtt
     1681 aagcaagcct atgattcgtt tagaaaatta ctcccagatc tttccgtttg taacttctta
     1741 gaagataaag aaggagctca atcaagtcgc atggtctttt caacttatcc gaccatgatt
     1801 ggagcgatta gtggtcaaga agaagtaaat caacgccctt tcactgttgg gcattttgac
     1861 cttatcataa ttgacgaatc tcaccgttct atttatcaga aatacaagtc catttttgat
     1921 tattttgatg caagaattgt aggcttaaca gctactccgc gtcaagattt agataaaaac
     1981 acctatggat tctttaattt ggagaatggg gttccaacat atgcatatga tttggaagag
     2041 gctgttaaag acggatattt agtagcctat cattctatcg aaaccaaact gaaactacct
     2101 acggatggtc tacattatga tgatttgtcc gaagaagaaa aggaacattt tgatagcaaa
     2161 tttgaagaca atagctgtga aaaagatatt gatgggagtg tatttaattc ctttattttc
     2221 aataaaagta cagtagaaat tgttttaaat gaactcatga caagaggaat tcagacagcc
     2281 tcgggtgatg aaattggtaa aactattatt tttgctaaaa atcatgatca tgcggaatat
     2341 atcagaggta tttttaacaa ccgctatcct gaaaaaggga gcgactatgc tcaggtgatt
     2401 gattatagta ttaagcatta tcagaccttg attgatgatt ttaaaattaa ggagaagtat
     2461 cctcaaattg cgatttctgt cgatatgtta gatacaggta ttgatgtacc agaggttgtt
     2521 aatttagtct tcttcaagaa agtacgctct aaaactaagt tttggcagat gattggtcga
     2581 ggaacccgtc tatgtaaaga tttatttgga cctgagcagg ataaggaaaa cttcttggta
     2641 tttgattatg gggacaattt tgattatttt cgtgcagatc caagagatgg agagggtcgt
     2701 cacattgttt cgctgactca gcgtttattt aatatcaaag tggacttgat tcgagaactt
     2761 cagggactcc aataccaaga agatcagttt gcgagagcat accgtcagca gcttgtctcg
     2821 gaacttcaag gtcgtataga gagcttaaat gagttggact tcagggttcg tatggtttta
     2881 gatacagttt atagctatag gaaattggaa agttggcaga atctaactgc tgttacaagt
     2941 gaaaccattc aaaaaaatct ctctccgctt ttatttgatg aagataaaga agatgagatg
     3001 gcgaggagat ttgatttgtg gttgcttcat attcagttgg ggcaactgac agctaaatct
     3061 tccactgttc atatttccca agtgatgaag acggctagag ctctttctgc tattggcaat
     3121 atcccgcagg tttttgagca ggctgaaatt atcaggaaag tacaggagcc tgaattttgg
     3181 aaagaagtta acttgtctga tttggaaaaa attcgtcttg ctattcgaga tttattacag
     3241 tttttggata aaacagaccg taaaccctac tatgttaact ttgaagatcg tatactctcc
     3301 actgttcacg agaccacagc atttttgcag gtcaacgatc ttcggtctta caatgaaaaa
     3361 gttgagcatt atttgaaaac tcatctggat gaggagtcca tttctaagct ataccataat
     3421 aaaaagttga catctgatga tatgcttgca cttgaaaaat tgctttggga aaaattaggt
     3481 agtaaagcag actaccaaag tcattatgaa aataaggcaa ttccgagatt ggttcgtgag
     3541 attattggct tagatagaga gtctgccaat cgtatttttt ctaaattttt gtcggatgag
     3601 aatcttaatg ccaggcagat ttcatttgta aaattgattg tagactacat tgtagaaaat
     3661 ggttttttag agacgaaagt gttaacgcaa gagccgttta aatcttatgg ttctgttcaa
     3721 ctactcttcc aacaccaact accagtactt cgtaatattg ttcaaatcat tgaacttatc
     3781 aataatcgag ctggagaagc ggcttaaatt ctaaagtgat tgccatgctg agactcattt
     3841 aaaattaaaa agagtagaaa tttatgctat atatgagaag ttttattagg aagaatgtca
     3901 tcgttttcct agaatacagt atcagttgtt aagtggttga taaatttcaa agtagatact
     3961 tgtaccacga tgtttgttga tcgagttatt aacaaaagag ctactttgat tttaaagaaa
     4021 tagaaaacaa aaagccgagc aagaattcaa ttgcaggaga aaatgaaata atactcaatg
     4081 aaaatcaaag agcaaactag gaaactagct gcaggctgct caaaacactg ttttgaggtt
     4141 gcagatggaa gctgacgcgg attgaagaga ttttcgaaga gtataaatct tcctaggata
     4201 aagcaaaacg catagtatca agggttttca acacttgata ctatgcgttt tctgatgtta
     4261 aagactttct accaggtttt ttaaaagcat aattgttagt tgtagtcatt tattattctt
     4321 caaagaaaaa tggtggggcg aattttttca gttcttcaaa gcacttttga gcagtatctg
     4381 catcttcaca gatgataaga cagacatcat taccacaaag ggtagcgata gcgtcaggga
     4441 agctcaaagt atcaatgata gaaccaaagg attgagccag tccaggaagg gtttttagta
     4501 ggacttggtg ttgaactggg cgcatccaga caagggcgtc ttccatgtag agttcgagac
     4561 gtttttccca ttttgagatg gaaccattgt taagaacata ataagcgcta tcttcttcgc
     4621 ggacttttga taggttcata tttttgatgt cgcgtgagag ggttgcctgg gttacttgaa
     4681 tgtcgttctc agcaagaagg gcttgcaact cagcctgtgt atgaatcttg ttttttgtga
     4741 taagagcgcg tataagttgg tggcggtgtt ctgatttatt cataataatg taactccttt
     4801 tagcaaggta aggtaagcat ggactgagcg aggtcgacag tcaagtggta gtctgtattg
     4861 tcacggatgg tgatttcaaa gtcagtagta tagaggacta aacggagagt gtctccttct
     4921 tttagcttgt aaatagttgg ctgcagttca aattgaacgt ccatccattc atctgcagta
     4981 atatcctcta ctaacagtaa atcatttcta ttttgtaaat taaggtaacc ttttgtcacg
     5041 actcgttgtg cctctggtct aaatggcaat tcacagagat tttccaacat gtgatagcga
     5101 ccgttgtcaa tggttctagc acttaaaata gctggataag gttgtaggta tttcttttgc
     5161 ccaaattcta gcagttgggc agataagagc cccttgtttg tactggattt gatacgaaga
     5221 ttgagctgag cgcgaccgtt taggtggaga tctttagtca caggaaggtt aatagtaatc
     5281 tgattggctt tcccttgata gagctctgta ttgaaggttt ggtatgtctt accatagcgc
     5341 tcaaaatcct tatctgggta ctggttttga atagcttgct cttcttgacc aagtgagaag
     5401 gtttcacagt tttcttgccc accgaagtta tcaagtgata accaagtctg tggagctgta
     5461 ttgtcctgcc agataacagt aggaagttga aagtctgttt cctgtcctag taatttcttg
     5521 gtcaataagg catttatgga ctcacggaag tcaattgatt gccaattgtt catgtaaaca
     5581 tgggcaccat tatggaaaaa gagatgcttg tgtatatgag taggaagagc atggaacatc
     5641 tggtaaacat gaagtggttt gacattccaa tcctgagaac catgagtaaa gacaacctct
     5701 gcctttactt tatgggcatt gagcagataa ttgcggtcat gccaaaactg attgtagtcc
     5761 ccagtttttc ggtctagctg agctttcact ttttctaagt cagcttggtg agcttcattg
     5821 ccacggatat agtcgccagc taagagatta cgagaatagg ttaactcagc aagggagtca
     5881 aagtcctcac ctggataacc acctgggcta gtcaccagac cgttttcacg gtagtagttg
     5941 taccatgatg aaattcctgc ctcggcaatg ataacttcta aaccatcgac tcctgtagtc
     6001 gcaagaccat tggacatggt acctagatag gaaagtcctg ttgtagcaac ttttccgttt
     6061 gaccaatcag ccttgacttg acgctggcgc gtgtgatcag taaaggcacg gcaacgaccg
     6121 ttaagccaat cgatgacatt tttataagcc tcgatttgct ggtagtctcc attagtcatg
     6181 aaacctgtcg agtctttggt accaacacct gagacataga gattggcaaa gcctctcgga
     6241 aggaagtagt cgtttagtgt atagctagag ttgatgtgag ttagcttttc ctcagcctct
     6301 gctataagct cagctttacc ttggggttgg acgagattta gttgaggttt ctctagctca
     6361 atcttgtgag gaagcttaac ctcaagctcg ccctccatct tgtagagagc cttgtcacta
     6421 gccttgtcat tggttccctg atgataaggg ctggctgtca tgatggcagg gatttttcca
     6481 tcaaaacgag ggcgaataat gctaaccttt actaggtctg atagcccttt ttggtcagta
     6541 tcgacacgag actcaacgta aacgacttca cgaatgacat cctggttaga aaaagtagcc
     6601 aaactcttgc cgttaaagta gtggtagtca ttatcctccg gaataagacc atcactaaca
     6661 agttggtcga taagagtatt tccttttttg gtgcgagtat tgagtaactg atagagattt
     6721 tcaatcaagt caccatatat aatgggaaat ccagtttctt tacgaaaaac gtcactatct
     6781 tcgaagtcaa ccaaataaga aaagcctaaa agttgaaaag caacagtata aaaaatatct
     6841 gctgtcagtt catcttctga ttgaaaaaat gtcagcaggt ctgttttttt atcagctgct
     6901 aggatagaaa gtgggtagtt ggtgtcttga taagtgaaaa agaaacgacg taaaaaggtt
     6961 tcaagtgagt ctttgtgatt ggctgtattt tgtaaatcaa agccacattt ttttagttca
     7021 gataagacat tttcttttgg aaaattgata taactatatt gattaaaacg catagaacct
     7081 ccatatagaa tgacagttaa ggttattata tcaaaaaaaa agcagaaagg gaattgttaa
     7141 cttcaaaagg aaataatcca ataaaaatga ataaagtact aaattcaata tagagaacag
     7201 agtaacaata agaataaata gatagggtat aaaagttcta ggagatttat attatatgct
     7261 ttctattttt atatacaata tagtataaat ataaaaatga tgacaaaaat acaaatgaat
     7321 agaaaataaa ttagtaagct gatgaaattt ttctcaagag aagccattta taggtgaaaa
     7381 tggtataata tagtgagaag gatagaggag aagtgtaaat tgatcgcaca actagataca
     7441 aaaacagtct atagttttat ggaaagcgtc atttcgatcg aaaagtatgt gagagcagct
     7501 aaagaatacg gctacactca tttggctatg atggatattg acaatcttta tggcgctttc
     7561 gactttctag agattacaaa aaaatacggc attcatcctt tgctagggct tgaaatgaca
     7621 gtgtttgtag atgatcaggg agtgaatttg cgctttttag ctctatctag tgtgggctat
     7681 cagcagttga tgaagctttc gacagccaag atgcaggggg agaaaacttg gtcagtcctg
     7741 tcccagtacc tggaggatat cgcggtcatt gtgccttatt ttgatagagt tgagtcgtta
     7801 gaactaggct gtgattacta tataggggtt tatccagaaa cactagcaag cgaatttcat
     7861 catcctatct tacctcttta tcgggtcaac gtttttgaaa gcagggatag agaagttctt
     7921 caagttttaa cagcgattaa agaaaatcta ccgctcagag aagttccctt gcgttcgaga
     7981 caagatgtct ttatatcagc aagttcttta gagaaactat tccaagagcg ttttccgcaa
     8041 gctttggaca atttagaaaa gcttatttca ggcatttctt acgacttgga tactagtctg
     8101 aaactgcctc gttttaatcc agctagacca gcagtagagg agttgagaga gcgtgctgaa
     8161 ctggggcttg ttcagaaggg gttgactagt aaagaatatc aagatagact agaccaagaa
     8221 ttgtctgtta ttcatgatat gggctttgat gattatttct tggttgtttg ggatttgttg
     8281 cgttttggac aatcgaatgg ctattatatg ggaatgggaa ggggttctgc agtaggcagt
     8341 ttggtttctt atgccttaga catcacgggg attgacccag tagagaaaaa tctgattttt
     8401 gaacgctttc ttaatcgtga acgctatacc atgcctgata ttgatattga tatcccagat
     8461 atttatcgtc cagattttat cagatatgtt ggtaataaat atggtagtaa acatgcggca
     8521 caaatcgtta ctttttcaac ctttggagcc aagcaagctc ttcgagatgt cttgaaacgc
     8581 tttggtgtgc cagagtatga attatctgca attactaaga aaatcagttt tcgtgacaat
     8641 cttaagtcgg cctatgaggg aaatctccag tttcgtcagc aaatcaatag taagttagaa
     8701 taccaaaaag cttttgagat tgcttgcaag atagagggct atccaaggca aacctctgtc
     8761 catgcggctg gtgttgtaat tagtgaccaa gatttaacca actacattcc tctaaagtat
     8821 ggtgatgaaa ttccactgac tcagtatgat gctcatggag ttgaggctag cggacttttg
     8881 aagatggact ttctgggact acgaaatttg acctttgtcc agaagatgca agagttgctt
     8941 gctgaaacag aaggtattca tctgaaaatt gaagaaatcg atttagaaga caaagaaacg
     9001 ttagctttat ttgcctctgg taatacaaaa ggtatctttc aatttgagca accaggtgcc
     9061 attcgtctgc ttaagcgtgt gcaaccagtc tgttttgaag atgtcgtcgc gactacttct
     9121 ctaaatcgac cgggtgctag tgactatatc aataattttg tggcaagaaa gcatgggcag
     9181 gaagaagtga ctgttctgga tccagtactg gaggatattt tggctccaac ctacggcata
     9241 atgctctatc aggagcaggt tatgcaggtt gcccagcgac ttgccggatt tagtcttggg
     9301 aaagccgata ttttgcgtcg ggctatgggg aaaaaggatg cctctgccat gcatgagatg
     9361 agggcttcct ttattcaagg ttcattagaa gctggtcata ctgtggaaaa agcagagcag
     9421 gtctttgatg ttatggagaa gtttgcaggt tatggtttta acaggtcaca cgcctatgcc
     9481 tactcagcct tggccttcca gttggcttat ttcaaaacgc attatccagc cattttttat
     9541 caggtcatgt taaattcttc caacagtgat tacttaatag atgcacttga agcaggtttt
     9601 gaagtagcct ctctatccat caacaccatt ccctatcacg ataaaattgc caacaaggcc
     9661 atctatctag gtttgaaatc cattaaagga gtcagtaatg atttagctct ctggattatt
     9721 gaaaatagac cttattctaa cattgaagat tttatagcta aattacctga gaattatctg
     9781 aaacttcctc tgctagaacc tttggtaaaa gttggtcttt tcgattcatt tgaaaaaaat
     9841 cgtcaaaaag tatttaataa cttagctaat ctatttgaat ttgtgaaaga gttgggaagt
     9901 ttgtttggag atgctattta tagttggcag gaatcggaag attggacgga acaagaaaaa
     9961 ttttatatgg aacaagagct tttagggata ggtgtcagca aacatccact acaagctatt
    10021 gcaagtaagg ctatttaccc gattacccca atcggaaatt tgtcagaaaa tagctatgct
    10081 attatcttgg ttgaagttca gaaaataaaa gtgattcgta ccaaaaaggg tgaaaatatg
    10141 gccttcttac aggcagatga tagtaagaaa aaattggatg tcactctctt ttcagactta
    10201 tatcgtcagg ttggacagga aataaaagag ggagccttct actatgtaaa aggaaaaata
    10261 caatcacgtg atggccgtct gcaaatgatt gcacaagaaa taagagaagc agttgctgaa
    10321 cgcttttgga tacaggtgaa aaatcatgaa tcggatcaag aaatttcacg cattttagaa
    10381 caatttaaag gcccaatccc agtcatcatc cggtatgaag aggaacagaa aaccatcgtt
    10441 tctccccatc attttgtagc taaatccaat gaattagagg agaaattgaa tgaaatcgtt
    10501 atgaaaacga tttatcgcta aaaatacgga aaatagaaga attttcaacg taaatgtggt
    10561 ataatcagta agaatgttaa aagaaaaagg agcataacca atatgaaacg tattgctgtt
    10621 ttgactagtg gtggagacgc ccctggtatg aacgctgcca tccgtgcagt tgttcgtcaa
    10681 gcaatttcag aaggaatgga agtttttggt atctatgacg gatatgctgg tatggttgcc
    10741 ggtgaaattc atcccctaga tgcagcttca gtaggggaca tcatttctcg tggtggtact
    10801 ttccttcact cagctcgtta cccagagttc gctcaacttg aagggcaact taaagggatt
    10861 gagcaattga aaaaacacgg aattgaaggt gtagttgtta tcggtggtga cggatcttac
    10921 cacggcgcta tgcgtttgac tgaacatggc ttcccagcta ttggtcttcc aggtacaatc
    10981 gataacgata tcgttggtac tgactttaca atcggttttg acacagcggt tactactgcc
    11041 atggacgcta tcgataagat tcgtgataca tcatcaagtc accgtcgtac ttttgtaatc
    11101 gaagttatgg gacgtaacgc tggtgatatc gctctttggg ctggtattgc aactggtgct
    11161 gatgaaatca tcatccctga agcaggcttc aagatggaag atatcgtagc aagcatcaaa
    11221 gctggttatg aatgtggtaa aaaacacaat attatcgtct tagctgaagg tgtgatgtca
    11281 gcggctgaat ttggtcaaaa acttaaagaa gctggagata caagcgacct tcgtgtaaca
    11341 gaacttggac atattcaacg tggtggttct ccaactgcgc gtgaccgtgt tttggcgtca
    11401 cgtatgggtg cacatgctgt taaacttctt aaagaaggta tcggtggtgt tgcggttggt
    11461 attcgtaacg aaaaaatggt tgaaaatcca attcttggta ctgcagaaga aggggcattg
    11521 tttagcctta ctgcagaagg taagattgtg gttaacaacc cacacaaagc cgatattgag
    11581 ctatctagct tgaataagag cttgtcataa tttacaattt agttaataag accaaaaagg
    11641 tcatatatat aaaggagtca caaaaatcat gaacaaacgt gtaaaaatcg ttgcaacttt
    11701 gggtcctgcg gtagaaatcc gtggtggtaa aaaattcggt gaggacggat actggggtga
    11761 aaaacttgat gttgaagctt cagctaaaaa cattgctaaa ttgattgaag ctggtgctaa
    11821 cacattccga ttcaacttct cacacggcga ccaccaagaa caaggtgagc gtatggcaac
    11881 tgttaaactt gcggaaaaaa ttgcaggtaa aaaagttggt ttccttcttg atacaaaagg
    11941 acctgaaatc cgtacagaat tgttcgaagg tgaagctaaa gaatattcat acaaaactgg
    12001 tgaaaaaatt cgtgttgcaa ctaaacaagg aatcaaatca actcgtgaag tgattgcgtt
    12061 gaacgttgct ggtgctcttg atatctatga tgatgttgaa gttggtcgtc aagttttggt
    12121 tgacgatggt aaacttggtc ttcgtgtggt tgctaaagat gatgcaactc gtgaatttga
    12181 agttgaagtt gaaaacgatg gtatcatcgc taaacaaaaa ggtgtgaaca tccctaacac
    12241 taaaattcct ttcccagctc ttgctgaacg cgataacgac gatatccgtt tcggtcttga
    12301 acaaggtatc aacttcatcg caatttcatt cgtacgtact gcaaaagatg tgaacgaagt
    12361 tcgtgcaatc tgtgaagaaa ctggaaacgg acatgttcaa ttgttcgcta aaatcgaaaa
    12421 ccaacaaggt atcgataact tagatgaaat catcgaagca gctgatggta ttatgattgc
    12481 tcgtggtgat atgggtatcg aagtaccgtt cgaaatggtt ccagtttatc aaaaaatgat
    12541 tatcaagaaa gtcaatgctg caggtaaagt tgttatcact gcaacaaaca tgcttgaaac
    12601 aatgactgaa aaaccacgtg caactcgttc agaagtatca gatgtattca acgctgttat
    12661 cgacggaact gacgctacaa tgttgtcagg cgagtctgca aacggtaaat acccactcga
    12721 gtcagtaact acaatggcta caatcgacaa gaacgctcaa gctcttctta atgaatacgg
    12781 acgtcttgat tcagattcat ttgagcgtaa ctctaagaca gaagtaatgg cttctgctgt
    12841 taaagatgct actagctcaa tggatatcaa attggttgta actcttacta agacaggtca
    12901 tactgcacgt ttgatttcta aataccgtcc aaatgctgac atcttagcat tgacatttga
    12961 cgaattgaca gaacgtggct tgatgttgaa ctggggtgtt atcccaatgt tgacagatgc
    13021 tccatcttca actgacgata tgttcgaaat cgctgaacgt aaagcggtag aagcaggtct
    13081 cgttgagtca ggcgatgata tcgttatcgt tgctggtgtg ccagtaggag aagctgttcg
    13141 cacaaacaca atgcgtatcc gcacagtacg ttaagaaaaa tataaaaacc tatcatatcc
    13201 agctttagag cttgtgtgat aggctttttg tatagagggt aagaaatagg caaaactttc
    13261 ataatggatt gatactcttc gaaaatctct tcaaaccacg tcagcgtcgc cttaccgtat
    13321 atatgttact gacttcgtca gttctatcta caacctcaaa gcagtgcttt gagcaacctg
    13381 cggctagctt cctagtttgc tctttgattt tcattgagta tgaaataaga tatgcacaaa
    13441 ttgattagaa agtcaaatga atttctacaa atgttttagc aatcgtaatg tacttgtcta
    13501 gattcgatct gatatatttt cgatttaatg atatggtatt taaaacctcc aaagtagctt
    13561 actccattct tttacttacg tgagtgtaga tgttatttac tgttttagcg tttttgtgtt
    13621 ccactctaac cattatagca ttcttctcag ctagtgtact aaggagtgtg tgcctgaaaa
    13681 tatgggaact aaggggctgg tttatcggtt tctctagttt agtatttgcc ttttgcaaag
    13741 tgatcttaaa tgcctttctc taaatttaca tatcactatt gtttaacaaa atctaatcta
    13801 ttttaggtca cttattcttt ttttgaaatg tagaatgaac tttttcaaag tttttcgaat
    13861 cttttaaaat ctgtttgctt tatatcgcca ttctcccccc ttttttaatt ctccctatat
    13921 agcctgacag ctttcccgat ggtacgaata tggttgcttt cgtctaggtg gatgtcgggg
    13981 tattcgggat tgagtttttt tgaggcagcc ttggcggagt ttcttgacat agttagtgcc
    14041 gtctacttgg aagatgccga tggtattata gtcaatctgt ggggtattct tgataaatag
    14101 gtagtcgctg tttcttatct ttggctccat ggacttgctg acgacataag cgattgggtc
    14161 gtagtcgtct gggataatgg aaactccata tctaaatcgt tgtcctgcat cgagcggcta
    14221 cctgcagaga taaactacct aacacgagag taagtagtct gtctgtagtc gtccagtctg
    14281 atgattttta cgatacttcg tttttctgat catacagttg cctctcggca taggtcagaa
    14341 ctttaccttg tctgggtggt tcccgttggt cgtagataga ttggatatcg ctaggagaat
    14401 ccttttgaac tggaggaaag agggcatcga tcaagctact gaatacttta actaagtcaa
    14461 atatagtatt tttcttagta gacctaaccc ttttttcata atttctaatg gtgtttttac
    14521 ttatacctat cttagtaccc aattcttatt gagtccaacc attactagtc tatattgttt
    14581 tatagttgat tgagtttgga atagtacgct gtagctgcta aaacatttct agaaattaat
    14641 ttgactttcc taatagagtt gttcatatct tatttcaatc tattatgttt ttcacctcta
    14701 acaatcgcaa tctcttcttt atacatgaat gaaatcgctt tctatttttg taagtaaagc
    14761 ataacacgaa atccacgaaa atgaaaacct ttgttgtgtt ttcgtaaaaa atttgttgac
    14821 agagcacgaa acgcctacta taattaaatc aagcttaagg aaataacaaa aaaacaaatc
    14881 ggagggaaag acgatgaaca cattaaatga gaaagtaatc aatatctgta aagcaatagt
    14941 taaagaaact ttaatccaag acatttagga gaaagaaatg aagaaatcaa gaaaattagc
    15001 cacattagga atctgttcag ccttgttttt aggcttggct gcttgtcaac aacaacatgc
    15061 tacttctgag gggacgaatc aaaggcaaag cagttcagcg aaagttccat ggaaagcttc
    15121 atacaccaac ctaaacaacc aggtaagtac agaagaggtc aaatctctct tatcagctca
    15181 cttggatcca aatagtgttg atgcattttt taatctcgtt aatgactata ataccattgt
    15241 cggctcaact ggcttatcag gagatttcac ttcctttact cacaccgaat acgatgttga
    15301 gaaaatcagt catctctgga atcaaaagaa gggcgatttc gttgggacca actgccgtat
    15361 caatagttat tgtcttttga aaaattcagt caccattcca aagcttgaaa agaatgacca
    15421 attgcttttc ctagataatg atgcgattga taaaggaaag gtctttgatt cacaagataa
    15481 ggaagagttt gatattctat tttcgagagt tccaactgag gcaactacag atgtcaaggt
    15541 tcacgctgaa aagatggaaa cattcttctc acaatttcaa ttcaatgaaa aagctcgaat
    15601 gctgtctgta gtcttgcacg acaatttgga tggcgagtat ctgtttgtag gccacgttgg
    15661 ggtcttagta cctgctgatg acggtttctt atttgtagag aaattgactt tcgaagagcc
    15721 ctaccaagcg attaaatttg ctagtaagga agattgctac aagtatttgg gcaccaagta
    15781 tgcggattat acaggcgagg gactggctaa gccttttatc atggataatg ataagtgggt
    15841 taaactttaa tagtgttatg atatgacagt aatcttagga tttttatttc tatttttgat
    15901 gagttggtgg gttatttcaa tcgttaatag ccagaaacca cgcaaaaatg aaacatttat
    15961 tggctatatc caaagatacg atattgatgg gaatggctat caatcattac cgtgtcctca
    16021 gaactgagag gagttcttga aatcgtaaaa ccactttgaa caagagttac ttcaacccat
    16081 tggctctgat ggattaagtt gttttcgtga ataccaaaat cagccgcaat ttcttcataa
    16141 gtgcggtatt ctcgcacata ttgaagagtg ggcataagaa ggtcttctag gcttaattta
    16201 ggttttcgtc caccttttgc gtgtttaagt tgataagctg tttttaatac agctaatatc
    16261 tcttcaaaag tcgtgcgctg aacaccaaca agacgcttaa atcgtgcatc agttagttgt
    16321 ttacttgctt cataattcat agaactacta taccatattt tgtttcgcag gaagtctaat
    16381 gctaaaatct tatcagtgag aagatttctt aaagtttatt ctttgtagtt tcatctgtat
    16441 tagtacataa tttaacttag agaaaatagg aggactaatc gaatggaatt aaaagatttt
    16501 acagaaaaag aacaggaaat gattaagaaa aggcttacaa tgtctaatat tagtgacaaa
    16561 gaaactactg agaagattct ggcgctagta ccacaagact tgattaagcg aatcccgttt
    16621 tttgtcagaa aacatgctac aacacgtacg attaaacgca tttcaattga acaccctgaa
    16681 ctctacgctg tagctcaaac aagtggtgag attccagaaa aaaaacgcga agaattgcgt
    16741 cagattatca caactatctt tgaacaaaag atgaataagc atagtattaa gtagagaatg
    16801 aaaaaatatt ttattggcgg tttgggaagc aatgcctatc atagcaagga ttttcttcaa
    16861 gaactagatt cgcaggtcta ttttctaaat ccatatgaaa agcatcttcg agatgaaaca
    16921 gaattgaaat catggtttaa aaatgagatt gtagaggaag aatctatctg tctgataggt
    16981 cattctattg gaggcgattt agctcgttat ttcgcatcgg aatttgaaga agtaaagaaa
    17041 ctgattcttt tggatggtgg ctatctagat ttagataaga ttttaccttt ggatacagag
    17101 ttagaggaaa ctaaaaatta tatcaaatct caaattgttt cggacttagc tcttcttatt
    17161 tctaaagaga agtccgaagc aaagcattgg tcagaaaata tggagaaagc tgtaagacag
    17221 tcctatcact ggaatgttga gtataataga tatgagttgg ctataaatta tgaaaatata
    17281 gaagcgatac tccgcctacg gagaaaaata caagctttta agagagaagt gggagatacc
    17341 ttgtttatta gtcctcgcta tcctaatgga gctacatgga gagaggaagc cctaaaagaa
    17401 ttgccagact attttaatac tattttttta gaaaactttg gccatgagct ttatactcaa
    17461 gcacctaaag aaatcgctag tctaatgaat gagtggctcg cttattttct atgagctact
    17521 tgtattgctg gaaatggtat aagatgaaaa tctacaagtc actaattttc atataacaaa
    17581 aaaacgaaca ggctcttttt ctgttattca gaagagaact tgttcgtttt tatcttgatt
    17641 ttcttaaaaa gctctatata catagggatt ttctggttta tcgactttgg taaagctgtc
    17701 gatttccaag gttggaaggt tttgtttgag ttctttatgg tagtgattga ccagttttcc
    17761 tttggctgta atccaggtgt tgttttcata ctgccgggta ttgcccttgg tcagcaatcc
    17821 atagacacca gaatctgcga tacagtggat aatgccgaat cggaacagaa attgactatt
    17881 ggcatgactg gggtcgttat agacaaagcc tgtaaactgg attgtcttgc cctcaaattc
    17941 atctggatag tcgtagatag cctccatgac ttccatatag ttttcattag tgatctgaat
    18001 actatcttgg gataagtatt tatccgccgc cgttcgcatt tccttttcat aggctgattt
    18061 tgaaaaataa gaactagtat ctggtttcaa atattggctt gtcgtccctt cacttgtctg
    18121 aatagctaga tccgttcctt ccgatagggg gaaatgataa cctttagcag aaacggtctg
    18181 agaatccaag ctaacagttg ggaaagttaa gccgatgaca agtggaatag ccagaagaga
    18241 aatactcgtc atcttggcca atcggctgtt cagatgactg tgggttttga cttgcttcat
    18301 ccagatatac aattgaacaa ttgccaagat aaaggaaagc accatggaga tataggccag
    18361 ataggaatag tgcatgttga tgtactggtt tagtttgccc gacagatgga ggtaaatagt
    18421 cagttcaaaa tagccagcta aaactaaaaa tcgaatcata ggatgactcc aatcaagaga
    18481 gaataggcta aaacgaccag agtcacaatc gtaatgaagt gactaataaa tcgtgctttc
    18541 aagtaatttt tcatcatgag aatatttttg atatccagca ttgggccaat gacgagaaag
    18601 gccagaactg gagccaaacc gaaactcgag agtagagaag cacctataaa ggcatccgcc
    18661 tcactacaga gcgaaagaag aaaggccaaa agcatcaaga gcaggatggc aaaaagagga
    18721 gtcgcactga tagaggtcag aatccgagtc ggaacgtaga cctgtattat agaagcaaag
    18781 aggcagccaa ataccaaata acgccccgta tcaaaaaatt catcaatggc ctgcacaaag
    18841 acttgaaaaa ctttttttgc agaactcaag taagaaaaat catgctcatg acaagccaga
    18901 cgattttctt tctgaatcgg ttcttgccag aaaaatccta gaaaaattcc tagtattaca
    18961 gccacaagaa tggaacccag agctcgtaat agggcgacat ggaaggagtt gccaaaggca
    19021 gaataggtcg caaaaagaac aatgggattg ataacaggtg ctgtcacaag aaaaggaacg
    19081 gccgtgtaac ttggaacctt tttttccaga aaacgattga tgatggggac gattccacat
    19141 tcacaagaag ggaaaagtat accgacaaag gtcccaaaaa agattctccc ccaacgattt
    19201 cgagggagaa aatgataaac cttgtcaggt gtgatataaa cttcaatcag ccctgagaca
    19261 atgcttccta tcagaacgaa gggaagggct tcaatgataa tggagagaaa aatggctcca
    19321 gtttgaagta cactagatgg gagttgttga aaagcagtca tctagttttt cttttctgct
    19381 ttgacttttt tctctttgtc atcagggaaa gtaacttgtt tcaaatcagg gagtttagca
    19441 aactcttcaa acatcttgtc caagtcgtct cgttttgtaa atttgacagc cataggtgta
    19501 cctcgattct agattctacc tctattatac tattattttg cggaaaaggg aaaagttaaa
    19561 atttcaaatt gagtagctaa gagataatat agcttgatat tacctgatat atatcataat
    19621 tgtgctataa ttaaataaaa agaaaatcat tatcagaaaa ttatgggagg tagtcaaact
    19681 gacttactca attagtgttg ttttgttaca tcaaggagat aaaaatagtt tattagagtc
    19741 tttgtcaata gtggagagac aaacattaaa agatatagaa gttattattg taaatcaatc
    19801 tacagaggag atagcttcca ttagccaaca gatgaatcta tcatttaata ttaagattat
    19861 agatgagaat tcttttgctg agttgtctga tatgattaca ggagattata ttacgtttct
    19921 atcttcaaat gattcattat ttgattggac atttgaaaag atgtatcatg cgataaaatt
    19981 atccgattct gatgttgttc ttggtcattt tgctgatttg gttgatggag tattttactt
    20041 ttatcctttt tggggggata attggttgac agaaaattta acgaatgttc aagttttaca
    20101 aaagatggat gatacagatc atcgaattag aaaacaatat cgctcattat ttggaaaatt
    20161 attcaatagt aagcttttac gaaaaataaa tcaatttgat ataaacaacc ttatttggag
    20221 actttatatt cactctaaaa ctgcaaccta tattaatttc ccgacatata tctataaacc
    20281 tgttatagat gctaaacctc taaaagactc ctacaaaatc atattagaaa attatgaaaa
    20341 tagaatcaaa gattgcgccg tcttagaaaa ctatgatata gaaagagcga aaaaacaata
    20401 tattcaagaa ttggcaaatt ttagtgcttg gttaaaaaat gacggtgaat atttagattc
    20461 tgatattgtt tatcataagt taatagctgc agagaaagga attttccctt ttcttgagct
    20521 tgatagatta gattttacga ttataagtaa taattgtgtt ggaggattaa tttataaaca
    20581 gcttggtttt caatatagaa caccttttgt tggtttattt attcttcctg atgattatta
    20641 taaacttaca aagcattttc gttattatat ggaacaagaa cttgttttcg aagaggaact
    20701 tatttcaccg agctggactc atgaagttta tcctttgggg cacttaggag atatagatat
    20761 tcattttcta cattattcta gtattgaaga agcacgaact aagtgggaaa aaagaaa
//
LOCUS       DAVHEX010000042        20190 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVHEX010000042 DAVHEX010000000
VERSION     DAVHEX010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20190
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.042"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(53..328)
                     /locus_tag="VVL45_001452"
     CDS             complement(53..328)
                     /locus_tag="VVL45_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU4187614.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(434..1273)
                     /locus_tag="VVL45_001453"
     CDS             complement(434..1273)
                     /locus_tag="VVL45_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU4187615.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRISRVTGLLSSLLNIRAVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            1415..2263
                     /locus_tag="VVL45_001454"
     CDS             1415..2263
                     /locus_tag="VVL45_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEU4187616.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSGWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(2356..3273)
                     /locus_tag="VVL45_001455"
     CDS             complement(2356..3273)
                     /locus_tag="VVL45_001455"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEU4187617.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            3711..3916
                     /locus_tag="VVL45_001456"
                     /pseudo
     CDS             3711..3916
                     /locus_tag="VVL45_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(4227..4520)
                     /gene="rpmA"
                     /locus_tag="VVL45_001457"
     CDS             complement(4227..4520)
                     /gene="rpmA"
                     /locus_tag="VVL45_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEU4187618.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(4537..4881)
                     /locus_tag="VVL45_001458"
     CDS             complement(4537..4881)
                     /locus_tag="VVL45_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEU4187619.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENY
                     SEFVQTRVITEN"
     gene            complement(4897..5211)
                     /gene="rplU"
                     /locus_tag="VVL45_001459"
     CDS             complement(4897..5211)
                     /gene="rplU"
                     /locus_tag="VVL45_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEU4187620.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYVEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(5247..5334)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(5393..6097)
                     /locus_tag="VVL45_001460"
     CDS             complement(5393..6097)
                     /locus_tag="VVL45_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEU4187621.1"
                     /translation="MGGKMRLLPIRKISRQSKRLALFLTFCAGYVDAYTFIVRGNTLV
                     AGQTGNVVFLSVELIKNNVSDVRDKVLTLLAFMMGVFLLTIYKEKLRIVKKPILSLIP
                     LAILSIIIAFVPQTVDNIYLVPPLAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLA
                     FGDYFRTKHTPFLREGFIFVSLLSSFVLGVVFSAYLTIFYHEKTILGVPIMMSVFYLS
                     MLFASWQKKVKEKASF"
     gene            6499..6798
                     /locus_tag="VVL45_001461"
     CDS             6499..6798
                     /locus_tag="VVL45_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEU4187622.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
     gene            6979..8305
                     /locus_tag="VVL45_001462"
                     /pseudo
     CDS             6979..8305
                     /locus_tag="VVL45_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(8567..9541)
                     /gene="pta"
                     /locus_tag="VVL45_001463"
     CDS             complement(8567..9541)
                     /gene="pta"
                     /locus_tag="VVL45_001463"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEU4187623.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
     gene            complement(9585..10481)
                     /locus_tag="VVL45_001464"
     CDS             complement(9585..10481)
                     /locus_tag="VVL45_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU4187624.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIYIPAEEGFETLEAIEHPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            complement(10478..11296)
                     /locus_tag="VVL45_001465"
     CDS             complement(10478..11296)
                     /locus_tag="VVL45_001465"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEU4187625.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            complement(11280..11951)
                     /locus_tag="VVL45_001466"
     CDS             complement(11280..11951)
                     /locus_tag="VVL45_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEU4187626.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            12079..12648
                     /locus_tag="VVL45_001467"
     CDS             12079..12648
                     /locus_tag="VVL45_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEU4187627.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDMTDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            12761..13720
                     /locus_tag="VVL45_001468"
     CDS             12761..13720
                     /locus_tag="VVL45_001468"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEU4187628.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            13730..14845
                     /locus_tag="VVL45_001469"
     CDS             13730..14845
                     /locus_tag="VVL45_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU4187629.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLKAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            14850..15197
                     /locus_tag="VVL45_001470"
     CDS             14850..15197
                     /locus_tag="VVL45_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEU4187630.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            15205..15420
                     /locus_tag="VVL45_001471"
     CDS             15205..15420
                     /locus_tag="VVL45_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEU4187631.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGKKVYQGLVGDLYRFLSHADFLHQN"
     gene            15503..16144
                     /locus_tag="VVL45_001472"
     CDS             15503..16144
                     /locus_tag="VVL45_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEU4187632.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            16162..16851
                     /locus_tag="VVL45_001473"
     CDS             16162..16851
                     /locus_tag="VVL45_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU4187633.1"
                     /translation="MKKPVIGITGNEKTHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(16848..17528)
                     /gene="radC"
                     /locus_tag="VVL45_001474"
     CDS             complement(16848..17528)
                     /gene="radC"
                     /locus_tag="VVL45_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEU4187634.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(17571..19862)
                     /gene="pcrA"
                     /locus_tag="VVL45_001475"
     CDS             complement(17571..19862)
                     /gene="pcrA"
                     /locus_tag="VVL45_001475"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEU4187635.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTAYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDSDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKPASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            complement(20053..>20190)
                     /locus_tag="VVL45_001476"
     CDS             complement(20053..>20190)
                     /locus_tag="VVL45_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU4187636.1"
                     /translation="RSKVENIFAKVKTFKIFSTTYRNHRKRFGLRMNLSAGIINHELG
                     F"
BASE COUNT         6213 a         4258 c         3662 g         6057 t
ORIGIN      
        1 aacctactga ccaagctaga aagcttgata caataggctt tttaaagact gattatttaa
       61 cagcgtcttt aagagcttta ccagctttga atgctggtac tttagaagct gcaattgtca
      121 tttctttacc agtttgtggg ttgcgacctt tacgttctgc gcgctcacga acttcaaagt
      181 taccaaaacc gatcaattga actttttcac cagctgcaag atagtcagct actgctgcaa
      241 atacagcttc aactgctgct gctgagtctt tcttagtcaa ttctgtagct tctgctactt
      301 tagcgatcaa atcttgtttg tttgccatgt taatgattcc tccaaataat ttctaattaa
      361 caaatataat catatcctaa aatccctttt aggtcaagtt aaaaacgcta tttcttccca
      421 ttttatttat tttttaggag tggtaacgta tcaaaatagc ccaagcgttc tcacccgtgt
      481 gagtttgaat aatggaaccc gtttccaaaa cagaaattgg cttttcaaca taagcttgta
      541 agctttcttt catctctttt gcccaatcat cactaccaga atatgaaatt ccaatctctg
      601 ctacagcacg ttcagaaagc gatgttatca actcatctaa ccatttttta aatgttttag
      661 ttccacgacc tttaaccatt ggctgcaatt catggtcttt catttgcatg acagcacgga
      721 tattgagaag agagctcaac aagccagtta cacggctaat tcgtccacct ttgacaagat
      781 tttccaaagt tgaaacacca atataaagct ctgtatggtt tttaacctct tctacatgag
      841 ataaaattgc ctccatatct ttaccttctt gagctaactt cgcagcctca acaacttgga
      901 atttcagggc ttggtcagtg aaggaactat caacaacagt cacatctgca gtagataggc
      961 tagcaccttg gcgtgctgct tctaccgtac ccgaaagagc atgggacata tgaatagcaa
     1021 gaatctggcc accatctttg cataggtctt caaaaatctc agcaaagaca cctacaggtg
     1081 gctgacttgt tttcggaaga ttcttacttt cttgcatcaa ctgaagaaat ttaccttctt
     1141 ctttcaaatc cgcatcagaa taaacaacat tatcaatcat tacagataat ggaacaattg
     1201 taatatctaa ttgctttact agttcaggtt caatagtaac agatgaatcg gttacaatct
     1261 taatttttgt catagtatca atctttctat tttaggattc agattggttt ccttacttct
     1321 aattatatca aaaaaaagat taaaaatcct aatggagtca atcaaatttt ccgtaaaatt
     1381 tgatataatc aacttataag aaaagaggtg tcctatgatt aaaaaaattt accccatttt
     1441 taccatttta ctaggtgctg ctatttatgc ttttggactg acttattttg tagttcccca
     1501 tcatctcttt gaaggagggg cgacaggcat taccctcatc accttttatc tttttaaaat
     1561 ccctgtttcc ctcatgaacc tgctgattaa tattcccctt ttcatcctag cttggaagat
     1621 ttttggagcc aaatccctct attctagttt actaggaacc ttagctttgt ccggctggtt
     1681 agcttttttt gagcatattc cccttcatat tgatcttcaa ggtgatttac taatcacagc
     1741 ccttatagcg ggaatcctat tgggaattgg ccttggaatt atttttaatg ctggaggtac
     1801 aactggcgga actgatattc tagctcgtat tctcaacaaa tacactcata tatccatagg
     1861 aaaactgctc tttatcttag atttttgtat tctcatgttg attctcctaa tcttcaagga
     1921 tttgagattg gtttcctaca cgcttttgtt tgattttatt gtttctcgtg ttattgattt
     1981 gattggtgaa ggaggatatg ccggcaaagg ctttatgatt atcacaaaac gtcctgacca
     2041 acttgctaag gcgattaatg atgacctcgg aagaggtgtt acttttattt ctggtcaagg
     2101 ctactatagt aaagaaaatt tgaaaatcat ctactgtatt gtcggaagaa atgaaattgt
     2161 gaaaacgaag gaaatgattc atcgaatcga tcctcaagcc tttataacta ttacagaagc
     2221 ccatgaaatc ctaggagaag gcttcacctt tgaaaaagaa taaaaagagg taatgtcgtg
     2281 acctcaaaag ttagactaaa tcatctatct tttgggttac agacaacctc ttttttattt
     2341 tatttactca agctcttaag accaattccg agttacttct tcatcagcct ttaactgatc
     2401 cactaattgg tcaactgagt caaatttggt catatctcga atgcgatcaa gccaataaac
     2461 catgacggtt tccccataaa tatcttgatt aaaatcaaaa atattgactt caaaacgtgc
     2521 ttcttctcca tcaaaggtca catttttccc gacactagcc atagcacgat acttctgtct
     2581 ttgaatctca acatcaacaa cataaacgcc atctgctggc atataagtac ggtctaaaag
     2641 cactaaattc gctgtcggat aaccaattgt acgaccacga gcattaccat gaaccaccat
     2701 acctcttgat ggaagcggtg cccccaaaag ttttcctgct tctttcacat ttccatctaa
     2761 aatagcttga cggatacgag ttgaactaat ctttcctttc tcatcttcta caggtggaac
     2821 aatgataact tctccatcaa agtaatcctt taaatcttct gctgtttttt tgtcagaacc
     2881 aaatgtataa tcaaaacctg caacaataat tttggcattc atagccttga tataagttgc
     2941 aaagaattct tgtgcagtga gactagcgaa ttgactacta aaatcaagga gatataattc
     3001 ttctacacct tcgcgcttta attttctttc acgttcagca gggttcaaaa tatgcaaaaa
     3061 caaatctgga tgataaggct ctaaagcgat ctttggagat tcattaaagg tcataacgac
     3121 gataggcaac aaatcctttc tcgcagcctt gttggcaaca cgaaataatt cttgatgccc
     3181 cttatgtatg ccatcaaaat agccgagaac aacgactgaa tcagatggtg tgccaatatc
     3241 tttttggttt tttataggaa tagtaataat cataaaataa ttatatcata gcgatagcta
     3301 tttctggaac agaaaatctg aaatgttgtt tttttcacat gaagtgtacc tgttttcaaa
     3361 aagcacttta ttctatcgtt gcttaactat gaactttgca atattcttct caaaaacttg
     3421 taggacatct tcaaaatttt gcaaggagtg attagacttg ttcggtaacc ataaagtgtc
     3481 atactatgct tatgtatgaa aaagcaatgc aactaactcc tgagaacttt aaattactaa
     3541 ttggtgccga aaaggtagaa tttagaatcg aggtacacct atggctgtaa aatttacaaa
     3601 atgagacaac ttgggcaaga tgtttgaaga atttcctaaa ctccctgatt tgaagcaagt
     3661 cactttccct aatgacaaag aaaaaagcca aaacagcaaa gaaaaactag atgactgctt
     3721 tccaacaact cccatctagt gtgcttcaga ctgggctatt tttctctcca tctgttagct
     3781 tggattctca gaccgtttca gctaaagaat atcttttccc ttatcagaag gaacggctca
     3841 agccattcag acaagtgaag ggacgacaag ccaatatttg aaaccagata gcagttctta
     3901 tagtcaattg aaataaaatc tgaagaaatc gagtaggaaa ctcatatcaa tgtttaacag
     3961 tgttctattc cagattcata ctcaatgaaa attaaagtgc aaactaggaa gttagccgca
     4021 ggctgtactt gagtacggca aggtgaaatt gacatggttt gaatttgatt ttcgaagagt
     4081 atcaatccac tatacatatt aaaaagtatg ggtaccaaaa caaaaaaaca ggctctccga
     4141 aaactcggaa agccttattt taagctgttt ctagcttcct cacctttaca tttcaaggtt
     4201 cgggataaaa ggttcaatgg acctttttat ttagcgattg ggtaaacaga aacttgtttt
     4261 ttatcgcgtc ctttacgttc aaagcgtact acgccttcaa ctttagcgaa caaagtatca
     4321 tctccaccac gaccaacgtt tacacctgga tagatgtgtg taccacgttg acggtaaagg
     4381 attgatccac ctgttacagt ttgtccgtca gctgctttag ctccaagacg ttttgcttgt
     4441 gaatcacgtc cgtttgatgt agaacctcca ccttttttgt gggcgaaaag ttgcaagttg
     4501 ttaagagtca tttttaacat aatgttttcc tccgtgttag ttttctgtga taactctggt
     4561 ttggacgaac tcagaatagt tctccgataa gtttgccata cctaagaaaa atgattcaaa
     4621 gaataactgg gtcatttctc tctggtgtga aggaagatct tttggtattt caaccatcag
     4681 atagccacct tcatcttcgt ttaattctag gattggttca tagcctgcaa atttctcaat
     4741 agaattgata aagttaatgg caagcgtaga aaccgatgca cacacgacat ctaagccgta
     4801 ttcgccactc tcggcgtgtc cagtaatttc cgcactcctc agctcgccat cttcggctct
     4861 ctcaaagact gcctgtatca tgtgttctcc ttaaaattaa gcgttgattg cgttgatgac
     4921 aacttttgta tatggttgac ggtgaccttg tttacggtgg ctaccttttt taggtttgta
     4981 cttgtaagta accactttct tttgttttcc ttgtttttca acagttccaa ctacagtagc
     5041 tccagcaaca agtggagttc cgacaacagt gttttcacca ccaacaagaa caacttcgtt
     5101 aaaagtaact tcttgaccag cttcaacgtt caatttttca acgtaaactg cttgaccaac
     5161 ttcaacttta acttgttttc cgccagtttt gataattgcg tatgtgctca ttatgcacct
     5221 cctatgattt ttatgggttt ccccgatttt ttgtgaagac tcgcctagca tcgtgggacg
     5281 aaccacttaa acttgaataa tcaagcaacg atgttcgtgc ggttgcacag gatcgtgcat
     5341 agtcaactct tcaagtatag catatctcct attttcttac aagtaataac acctaaaatg
     5401 aagctttttc ttttactttt ttctgccaag aggcaaaaag catgctgagg taaaaaacgc
     5461 tcatcataat aggaacacca agaatggtct tttcatgata gaaaatcgtc aaataggctg
     5521 aaaagacaac gccaaggaca aaactactaa gcaggctaac aaatatgaat ccttcacgca
     5581 aaaaaggagt gtgcttggtt cggaaataat ctccaaaagc cagcatggtc cgtttgatat
     5641 tccctgtcat aaaagcgtta ttataggcaa tacccgacac ttctccaaaa gcagttgtca
     5701 ccagtcccat acagaaggcc aagggcggca ctagatagat attatccaca gtttgcggca
     5761 caaaagcaat aatgattgat aagattgcca agggaatcaa ggacagaata ggttttttca
     5821 caattctcaa tttttcctta taaatcgtta ataaaaagac tcccatcata aacgctagca
     5881 aggtgagaac cttgtcccta acatccgaaa cattattttt aattaattct actgaaagaa
     5941 agacaacatt tccagtttgt ccagctacaa gggtattccc gcgaacaata aaagtgtaag
     6001 catccacata tccagcacaa aacgtcaaaa aaagtgctaa ccttttagac tgacgtgata
     6061 tttttcttat aggtaataac ctcattttac ctcccattgt attttctctt agaaatattg
     6121 taccattttc tttctaaaaa atcgtaggct accatttaga ttttactatt agcataaaaa
     6181 taataataga caactattta tccaaaaata gatagatgta acatgtttgc aaacaaagca
     6241 tacgaacctt tagtaaaatc atttccatga aactagaata gagccctctt agcaaaaatc
     6301 attattttaa tttatttcta atcactcctt gacataaata actctcacca ataaaagact
     6361 atgtcttaaa aaaatggtat aataaaatca atacttgggc ttgatggcta tgctactaat
     6421 aacaattagg agagaaaatc aggcacttgt taacaacaag gattatcccc ttgagatgaa
     6481 aggaacttta gaaatcttat gatgaacatg caaaacatga tgcgtcaagc acaaaaactt
     6541 caaaaacaaa tggaacaaag ccaagctgaa cttgctgcta tgcaatttgt tggcaaatct
     6601 gctcaagatc ttgtccaagc gaccttaact ggcgataaga aagttgtcag cattgatttc
     6661 aatccagctg tcgttgaccc agaggacctt gagactcttt ctgatatgac cgttcaagcc
     6721 atcaactctg ctcttgaaca aatcgatgaa actaccaaga aaaaactggg tgctttcgct
     6781 gggaaattac ctttctaaaa acaaggagct agaacaatgc ttgtcgataa caaaggctaa
     6841 gaaaggtgca aaaatgactc tataatattt gtagtgggta aatcccctat ggatattatg
     6901 gagcctattt ttgtgtagaa aaaagtccca tatgacctat aatgaaaagc gacaaaacaa
     6961 ctcattagaa agaatcatat ggaacaatta cattttatca caaaattact agacattaaa
     7021 gaccctaata tccagatttt agacatcgtc aataaggata cacacaagga aatcatcgcc
     7081 aaactggact atgaagctcc atcttgtcct gagtgcggaa gtcaaatgaa gaaatatgac
     7141 tttcaaaaac cttctaaaat tccttatctt gaaacaactg gtatgcctac tagaattctc
     7201 cttagaaagc gtcgtttcaa gtgctatcat tgttctaaaa ttatggttgc tgaaacttct
     7261 atcgtcaaga agaatcatca aatccctcgt atcatcaacc aaaagattgc tcaaaagtta
     7321 attgaaaaga tttctatgac tgatattgcc catcagcttt ccatctcaac ttcaactgtc
     7381 attcgaaagc tcaatgactt ccactttgag tgtaatttta gaaatctgcc taagattatg
     7441 tcttgggacg ttgaaacagt ccggggagtg actgtttcaa tcgggagatg gagatgagct
     7501 ttattgcgca agattttaac aatctcaata tcatcactgt tcttgaaggt agaacacaag
     7561 ctgtcatccg agatcacttt cttaaatatg atagagccgt ccgatgtcgc gtcaaaatca
     7621 ttactatgga tatgtttagc ccttactatg acttggctaa acagcttcgc tttcaaattt
     7681 ctaggctcag gctgaaacag tctcccaggc tgtttcactc ccgaatgcta aaatcgttct
     7741 tgatcgcttt cacattgtac aacatcttag ccgtgctatg agtcgtgtgc gtgtccaaat
     7801 catgaatcag tttcatcgaa aatcccatga atacaaggct atcaagcgct actggaaact
     7861 cattcaacag gatagccgta aactgagtga taagcgattt tatcgtccta tttttcgtat
     7921 gcacttaacc aataaagaga ttttaaacaa gcttttgagc tattcggaag acttgaaaca
     7981 ccactatcag ctctatcaac tcttgctttt tcacttccag aataaggaac cagagaaatt
     8041 tttcggactt attgaggaca atcttaagca ggttcatcct atttttcaga ctgtctttaa
     8101 aaccttcctc aaagataaag aaaagattat caacgccctt caactacact attctaacgc
     8161 caaactggaa gcgaccaata atctcatcaa acttatcaag cgcaatgcct ttggttttcg
     8221 gaactttgaa aacttcaaaa aacggatttt tatcgctctg aatatcaaaa aagaaaggat
     8281 gaaatttgtc ctttctcgag cttagctttt cttcaaccca ctacagttga caaagagcca
     8341 aaaaattctc ttttcatact catttaaaat caaaaaggga atttatcgtt ttacggatat
     8401 gtatattaca gatttctata ggtcgtgtat agacaggata tcatcgcttg gaagttttcc
     8461 tttttgacac caatcagtaa tttagttctc aggaattaat ggcagtgctt ttcgtacata
     8521 gtttcataac tacagtatag catagtatat cactttatag ttttcactat tgatgaactg
     8581 cttgagctgc tgtgatgagg gtcaacttgt aaacatcatc tgcattacat ccacgagaaa
     8641 gatcattaac tggcttgttt aaaccttgca aaacaggtcc tacagccgca aagccaccca
     8701 ggcgttcagc catcttgtaa ccgatatttc ctgcctcgat acctgggaag atgaagacat
     8761 ttgcttgacc agctactgta cttcccggag ctttcagagc tgcagtttcg ggaacaaagg
     8821 ccgcatcaaa ttgcaactca ccatcgattt caaggtcagg acgcaagtcg tgagcaattt
     8881 tagttgcttc aacgacctta tcaacgcttt caccaaaccc tgaaccttta gtagaatagc
     8941 tcaacatggc aattttaggt tcgatgccaa acatcttagc tgtgattgct gagttgatgg
     9001 caatttcagc caaggcttct gcatctggat tgatattaat ggcacagtct ccaaataggt
     9061 aacgttccgt accacgaacc atgaggaagg ctcctgaagt acgagttaca tttggacgag
     9121 ttttgatgat ttgtagagct gggcgaactg ttgaagctgt tgagtgaatc gctcctgaca
     9181 ccattccatc aaccaagccc aagtaaacca acatcacacc aaagtagttg acatcttcaa
     9241 ccaaaacctt gcgtacatct tcttcagtca ttttgccctt gcgacgctcc accaaggcag
     9301 aaaccatttc ttcaaattga ggataatgtt gagggtcgat gacctcataa ccatccatga
     9361 ttccttcaat ttcaagataa attttaattt tttcaggatt tccaagcaaa acaggaatca
     9421 cttctgtttc ttttactaag cgttttgttg cttgaagaat acgaggctct tccccttcag
     9481 ggagaacgat acgagcattt ttaccaacaa ggttggcttt gagactttca aaaacttcca
     9541 tgagttttct cctttaagat aataattata ctctgagaca gtttttatag agtattagtt
     9601 gataactggg taataaggtt actgaaatca tccggcaagg gactttctaa ctgcaagtct
     9661 tgctctaaaa atggatgata aaaggatagg taatggcaat gcagagcctg acgttgaata
     9721 ccatcttcca gactaccacc atacaaatca tctcccagca aaggaaaacc gatatgagaa
     9781 aaatggactc ggatttgatg ggttcgacca gtgtgcaggt gaatatagac caagtgaata
     9841 tttccataag aagctacaat cttgtatgaa gtatgggcat actttccgcc tttagccact
     9901 cgtctggtaa taatggaatc ttcatcacgc gcaatcggag caataatttc cccttctggc
     9961 tccaaatgtc catctccctt aaccaaagca aagtagcgtt tctcgataga tttcttctgc
    10021 aactgcttgt ctaatcgtgc atgggcataa ccgtgcttgg caaagagcat caagccagaa
    10081 gtgtccctat ctagtctggt aacaatgtga acctgctgat tttcataatt ttgcttgaca
    10141 tagtaaccct tgataaaatt ggcaatggta ttagagtgat tgacactagg aatagaagcc
    10201 actccatagg gtttattcaa gactagaaag tggtcatcct catagagaat atctaatgga
    10261 tgctcaatag cctccaaggt ttcaaagcct tcctcagcgg gaatgtaaat ggtaacgtag
    10321 tctccaacgt ccaatagata cgttgcattt tgcggttgat tattgaccag aatagctcca
    10381 cctcgaaact taatcttggc cagcaatccc ttagaaacct cgtgcttttt taaaaaggtc
    10441 ttaaccttga catgttcatc tgcgataaat tcaaacctca ttcatccacc tcaccgataa
    10501 aggcatcctt aacacggttc cagaaactgg tatggctagg agtcgcgaca aagtgaatct
    10561 tatgatggtc gatttgatac tcaatacgct caatattacg gaaagaataa acgctattgt
    10621 caaccgaaat agtatgataa tcgtttcttg ttggaataag ttcaatctta tccttcttag
    10681 gcacaataat ggaagagccc agtgttcgat aaacacgatt attaaggctg gcaatttccg
    10741 ttaattgcaa agcttcaatg gtagggtgta aaacagcacc gccaagagac ttgttatagg
    10801 cagtactacc agtcggtgtc gaaactgtta gcccgtctcc acgaaaacgt tcaaagggaa
    10861 caccatttat tacaatatct gccaccatgg ttcgatcaga cctgcggatg ctggcttcgt
    10921 tgagtgctct gaaaatctta acttcaccat tttcaagaaa gaccttcaca ttcagaacag
    10981 ggtaagaaac ccttgcccca gtatctagct gcaaattagt cactagcttg tccaactcaa
    11041 aatcacgata atctgtatag aagcccaaat gtccagtatg aagaccgata aagcggacct
    11101 tgtcaagctg attttcgtac ttatgaaagg ccgacaagag cataccatcc ccgccaatgg
    11161 aaatgacaat atccggattg gtatcattga gtataaactg atttctcttc aaacgatctc
    11221 gcaattcata caaaaccctt tgactctgcg gttttctatt ggctatcaga tcaattcgtt
    11281 tacctgtatt cttcatctgt atcgtcactg tttcctacac cgtcatttaa ttttctactc
    11341 aaaggatcaa aaagtgcctg ggcttcttgg atatcatcac gaatttcacc catttcttca
    11401 tccaactgat gggcgattct agctgtaatt tccagtcgct tcttaatctc atctgggaaa
    11461 tccccttggt acttgtagtt gagagaatgt tctatcgttg cccagaaatt catggccaaa
    11521 gtacgaattt gaatttctgc caaaatagtc ttagctccat tgatggtatc aaccgtatat
    11581 tctactacca catgatagga acgatagcct gatgcttttc tatgagtaat gtaatctcgc
    11641 tcctgtatga ttcgcatatc ctgacgcttg tgcaaaatat ccactacttc cttgacgtca
    11701 tctacaaact gaaccatcac acgtaagcca gcaatatcct gcaaatcgtg ttccaaggtc
    11761 gcataagtaa tgccacgacg agccattttt tctttgatgc tctcaattgg cttgactcga
    11821 ccggtcacaa actcaattgg agaatgctta ttttgcttac gatattgctt acgaatacca
    11881 cgaagtttaa tctttaactc accaacagct tgaatgtaag gatctagaaa ttcttcccat
    11941 tctaaggtca tatattctcc cttgttctca tattatccgt caaaaatatc tttgattttt
    12001 tctccagatt catatacaat aaatacaatg cttattatac cataaatccg ctttcaacga
    12061 taaaaaaagg taagaaaaat gaaacattta gaaattgaat tgaaaacact attgaaaaaa
    12121 gatgaataca atcgtctaaa agaccagttc acaggtgtca ctcctgttct tcaaacaaat
    12181 tactacatcg acacgcctga ttttgaactg cgagaaaaga aagtcgctat gcgcattcga
    12241 acctttgaag actgggctga gttgacactc aaagtcccgc agagtgttgg aaacatggaa
    12301 tacaaccaaa aattgcaact aaaagatgct gagaactatc tgagtaagga agaacttcct
    12361 caagggctag tactggatga attggcgaaa catggtatcc aaagtaagaa ctggcaggtt
    12421 cttggttgtc taacaacgct tcgctatgaa atgaaaacag ctattggtct catggcactg
    12481 gatcagagcc gatactttga tatgacagat tacgaattgg agcttgaagt ggaaaatcat
    12541 gagcaaggca aacaggattt ccgacaattt ttagagaaaa atcagatttc ctaccaaaaa
    12601 gctccttcaa aattggttcg atttgtcaaa agtatgaaaa atagctgaaa taatctccat
    12661 tttttggtaa aatagaaaag ataaaaatac acaaatccca gttcatatat gtaaggtaaa
    12721 tataactagg atttataaag tttacagagg acggtctata atgtcagata gaaaaaacat
    12781 gaaacttttc gcactcaact ctaaccaaga gattgcacaa aaaattgccc aagctgttgg
    12841 tgtcccactt ggaaaactat catcacgtca attttcagac ggagaaatcc aagtaaatat
    12901 cgaagaaagt gtccgtggtt atgatgttta catcatccaa tcaacaagtt tccctgtcaa
    12961 caaccaccta atggaattgt taatcatggt cgatgcttgt gtgcgtgcaa gtgcccacag
    13021 tatcaacgtt gtccttccat attttggcta tgcacgtcaa gaccgcattg cttgtcctcg
    13081 tgagccactt acagcaaaac tagttgccaa tatgctggtt aaggctggag ttgatcgtat
    13141 cctgactctt gatttgcatg ccgttcaggt tcaaggtttc tttgatattc cagtggataa
    13201 tcttttcact gttcccctat tcgcaaaaca ttactgcgat aaaggattgc ttggttcaga
    13261 tgttgttgtc gttagcccta aaaattcagg tgtcaaacgt gcgcgtagcc tggctgaata
    13321 tcttgatgct cctatcgcca ttatcgacta ccctcaagac gatgcaactc gtaacgaagg
    13381 ttatattatt ggtgatgttg aaggtaagaa agctatcttg attgatgata ttttaaatac
    13441 aggacgtacc ttctctgaag cttctaaaat cgttgaacgt gaaggagcta cagaaattta
    13501 tgctgtttct agccacggtc tcttcgtcga gggagctgct gagcttcttg acaatactaa
    13561 tattaaagaa attcttgtga ctgattcagt agcaacaaaa gaaaaaactc ctaaaaatgt
    13621 atgctacatc actgctagtg agttaattgg tgatgctatc gtccgtattc acgaaagaaa
    13681 accagtcagc ccactctttg cctacaataa aaagaaataa ggtgattctt tgatttattt
    13741 ggacaatgct gcaacgactc ctatgtcagc agttgctatt tcagctatga ccaaggttat
    13801 gcaagaaacc cacggaaatc cttctagtat tcatggtcat ggtcgtcaag ctggcaaact
    13861 cttgcgagaa gcccgtcagg aactagccca gttactaagg acaaaacctc aacatatctt
    13921 tttcacttct ggtgggactg aaggcaataa tactaccatc attggctact gtcttcgtca
    13981 ccaagaacaa ggaaaacata tcatcacaac tgccatcgag caccatgctg tccttgaaac
    14041 aattgattac ttggttcaac actttgggtt tgaagcaacc attatccagc cagaaaatca
    14101 agaaatcaca gcccagcaaa ttcaaaaggc tttacgtgac gatacgattt tggtttctac
    14161 tatgtttgcc aataatgaga caggaaacct acttcccatc gctgaaattg gccaaatact
    14221 caagcaacac cctgctgcct atcatgttga tgcagttcag gctattggta aaatcccaat
    14281 tcattcagaa gaattgggca ttgattttct cactgcttct gcccacaaat tccatggtcc
    14341 taagggaatc ggttttctct acgcatctag catggacttt gattcctatc tacatggcgg
    14401 agatcaggaa cagaaaaaac gtgcaggaac tgaaaatctg cctgccatcg taggcatggt
    14461 tgcagcccta aaagaagacc tagaaaaaca agaagaacat tttcaacatg tacaaaatct
    14521 agaaactgcc tttctggcag agctggaggg cattcagtat tacttgaata gaggaaaaca
    14581 tcatctccct tatgttctca atattggatt tcctggtcag aaaaatgacc tcttactcct
    14641 tcggctagat ttagctggaa tttcaatctc tactggctca gcctgtactg caggcgttgt
    14701 ccaatccagc catgttctta aagccatgta tggcgcaaat tcagaacgct tgaaggaatc
    14761 ccttcgcatc agtttgtcgc cacaaaatac cgttgaagac ctacaaaccc tcgcaaaaac
    14821 cttaaaagaa attatcggag gttagccata tggcatttga aaaaatcatt cagttaaaaa
    14881 attgtcgtta cgattacact cttagccctt ctgttaaaaa attcaccctc aaagataaca
    14941 ccttttttga aactaaggtt ggtaactatg aactgactcg ccttttggaa aaagtgccaa
    15001 acagcggtga aggcttccaa ctcaaaatca tcattaacaa ggaacttaca ggggctaaaa
    15061 tcaatatcac tgacaagttt ggccttcgtc tagttgatat tttcaaatca gaagaccacc
    15121 atattcatca ggaaaaattc tacttcctca tggatagctt ggtagaacgt ggtgtcttta
    15181 caaaatcgga aagatagagc caaaatgttt gaactgacct ataaggactg ctatcatgta
    15241 gagcgtactc tcaagtatga ggatcatgag gctctcatgt tgactttgtc aggatgtgta
    15301 accttaccag atacacttta tgtgacttct ctgactttta ggggcaaaaa agtttatcag
    15361 gggctggtcg gagacctcta ccgttttcta tctcatgcag attttttaca tcaaaactaa
    15421 gatttttctt agttttttct tatttttgtt gattttttca caatatttct ataaaataga
    15481 agaatagaaa ggttgtgatt ttgtgaaaga taaacagttt gctattccaa aagctacagc
    15541 aaaaagactc tctctctact atcgaatttt taagagattt catgcagaaa agattgaacg
    15601 tgccaactct aagcaaattg cagaggctat cggtattgat tcagcgaccg tacgtcgtga
    15661 tttttcctat tttggtgaac ttggtcgtcg tggttttgga tatgatgtca aaaaactaat
    15721 gacatttttt gccgatttgc tcaatgacaa ctctattacc aatgtcatgc tggttggtat
    15781 tggaaatatg ggccatgccc ttctccacta ccgcttccac gaacgtaaca agatgaagat
    15841 tatcatggcc tttgacctag acgaccatcc tgaagtcggt acccaaactc ctgacgggat
    15901 tcccatttac gggatttctc agatcaagga taaaatcaag gatgctgatg tgaagactgc
    15961 tatcctaact gttcccagcg tcaagtcaca agaggttgct aatctcttgg ttgatgctgg
    16021 tgtgaaagga attctcagtt tttcaccagt ccatctgcat ttaccaaaag acgtggtcgt
    16081 tcagtatgtc gatttgacaa gtgaactcca aaccctcctc tacttcatgc gaaaagagga
    16141 ttagaaagcg aaaatcattt tatgaaaaaa ccagttattg ggattacagg aaacgaaaaa
    16201 actcatccag atgatgacat catgatgagc tacgcagcaa aaggctttgt tgaaggcgtt
    16261 aaagatgctg gagggattcc catcatccta ccgattggtg atcaagaaat ggcctgccac
    16321 tatatcagtt tgattgacaa gctcatcttg acaggtgggc aaaatgtcga tccaaaattt
    16381 tatggcgaac ccaaaaccat tgatagtgat gactatcacc ttcaaagaga tatcttcgaa
    16441 ctggccctca tcaaggaagc tattaaacag aaaaagccca ttttctctgt ctgccgtggt
    16501 acccaactct ttaacgttgc catgggtgga actttgtacc aagatatcga agaccattgg
    16561 caggattctt ctgttgagta cacaactcaa cgcttggtaa cagaaccaga taccgttctc
    16621 caagaaatct atggagaaat ctctcatatc aactccttcc accaccagag catcaaggac
    16681 ttagcaccaa atttaaaagt tgtggctcat gatcctaaag atggtatcat tgaggctgtc
    16741 atgagtacgg atgatgtcgc ctttctcggt gtccaatggc atccagaact tctttttgaa
    16801 aatcgtccca aagataaaaa actctttgac tatgtcgtta atgaacttta gattaaatct
    16861 gtcttttcac gataactaaa gtaattagaa tgagagacaa tcaaatggtc caagagaaca
    16921 atccccatca attcgcaggc ttctttaaca agtttagtga catgatcatc attttggcta
    16981 ggcgctaccg ctcctgaagg atgattgtgg accaagataa gagaagtcgc catatgcttg
    17041 attgcatagt gaagaatctc tcgcggttca gcgatactac gagttacaga cccgataaaa
    17101 atggtctgct gatggatgat ttgattttga gtattgagat agagtgccac caggtgctct
    17161 tgttttttat cccctaattc ctgctgcatc ttcttggcca acttttgact gctgagaata
    17221 ctttccattt caagagtctc gtgtttgtga atacgatgcc ccagttcaat catagcttgt
    17281 aattctatgg ccttaacacg cccaatacca gacaaactct gcaattcctg cagggtcatt
    17341 tttttcaaat ccgttaggct tgaaagattg ttcaagactt tttgggcaat ttcaaaaacg
    17401 ctagcttgac gtgttcctgt cctgagtaaa atagctagca actcttggtt actaagcgct
    17461 tcaactcctt ccttggccag cctttctctt ggtaatagtg aatcttcttg gaatgaaata
    17521 ctgtacataa aaatcctcct cactttatta ttcgtgagaa ggatggaaaa ttagattttt
    17581 ttctcaattg gagccacact ggctaaaagt tttttcaaac ctacttctgg gaaattgatt
    17641 ttcaattcct gcctagcacc gctacctgaa acttccagaa cggttccctc tccccatttc
    17701 ttgtggagag caatatcacc aatggaccaa tttgcctcgc tagatgctgg ttttgcgcca
    17761 gctgtaaatt gaccaaatgg aagaccgctt gactggattg attttggggc agcaccgcgt
    17821 ttacggtctt gaagagcctg agccaaactc ataccttgac caaaggaaat actaccactg
    17881 ctatatgatg ccttaaagct tgtatttgca ggacgagcca gaccttgata ctcaagcaag
    17941 tctgaactga tttcgttaat aaaacgagtc ggacggttat aattggtacg accaaaaagc
    18001 aagcgtgagt tggcattggt cagatagaga attttctctg cacgcgtgat acctacatag
    18061 gctagacggc gctcttcttc taattcatct gaatcttcag tcgcacgact aagtggaaag
    18121 acattttctt ccatcccaat caaaaagaca actggaaatt cgagaccttt ggcagcatgc
    18181 agggtcatca aggtcacttc tgatgtctcc tgactacctg aatctgtgtc ggcaatcaaa
    18241 gccaagtcat ttaagaaacg actcagtttg tccagaccag tttcctcttc tgtcacatcc
    18301 gtggtgtcat caaagttctt cgtaacagaa agaaactctt cgatattttc aacccgtgcc
    18361 ttgctttcta gagtcgcttg ggcgttaaga atatcgacat aacctgtttt ttctaggacg
    18421 gactcaacca actctgtaat gcttaagtgg tctagctgct cccgcaaatc aagcatcata
    18481 ttggcaaaat cccagataga ttgggctgcc ttacccttga taccagacaa cataatatta
    18541 gcagaagcat ccagcataga catattttgc aaatttgcaa aatcacggat tttctcaact
    18601 gtacctagac caattccacg tttaggctcg ttgataatac gctcaaaact aatattgtca
    18661 ctcaaattag caataaggtt gagataagca ataatatcgc gaatttcctt acggctgtag
    18721 aatttggttc cgccaaccat ggtataagga atgttagact tgagcagggc ttcctcaatt
    18781 gtacgggact gggcattagt ccgatagaga actgcaaaat ccttatgaag gaagttttga
    18841 ctgcgactaa gttcatcgat ggttctggct acaaatacag cctcatccag ctcatcatcg
    18901 gcacgatagt aaacgatttg ctccccatca gcgttttgag tccagagatt tttaggacgg
    18961 cgatttttat tatttttaat aacctcgttg gccgcttgga gaatggtttt ggttgagcgg
    19021 taattttcct ccaacaaaac aaccttggct ttggggtaat ccttttcaaa gtccaagata
    19081 ttctgcatat cagcaccacg ccaaccgtag atagactggt ccgcatcccc aaccacacag
    19141 atatttttaa aacgggaagc caagagtttg accaattggt actgagcgtg gttggtatct
    19201 tggtactcat caacgtggat gtattggaat ttttgctggt agtaggtcaa aacatcagga
    19261 ttttgatcaa agagacgcaa ggtcagcata atcaaatcat caaagtcaac ggattcagac
    19321 tgacgaagtt ctttttgata ggctgtataa cactgggcca caatttgcgt atacatatcg
    19381 ccagcttggg cagcataagc aacatcatca atcaaatcat tcttagcatt ggaaatggtc
    19441 cccaaaatag ttcgttcatt ccatttttta gggtccaagt tcaactgttt gagaatacgt
    19501 ttcatgagcg ttcgctgttc accaggatcc acaattgtaa aattacgatt gtagccaata
    19561 tggtccgcat cgcgacgcaa aatacgcaca cacatggagt ggaaggtcgc aatcagacag
    19621 tcctgagtcg ctggattgag gctataagca cgctctttca tctcacgcgc agccttgttg
    19681 gtaaaggtaa tggccaagat attccaagga ttgaccagct tttcatcaat caaataagcg
    19741 atacggtggg tcaaaacacg agtctttcca gaaccagccc ctgccatgat tagcaaggga
    19801 ccttctgtcg tttgcaccgc ctcagcctga cggtcattca ttccatttaa taatgcgttc
    19861 atcttctctt ccttactctt gaagtcaata tttctattat atcagaaatc agggaaaata
    19921 gaaactttac ggggagtagc aaaatatagt ggattgaaac tagaatacta cactgtgggt
    19981 ttctaaaaca ctcgactttt cacccaattt tttcataaac tagctcaata cctcaataga
    20041 cttcctgcaa aactagaatc ctagttcatg attgataata ccagcactca aattcattcg
    20101 taatccgaag cgtttacgat gatttcgata ggttgttgaa aatattttaa acgtttttac
    20161 tttggcaaag atgttctcaa ccttgcttct
//
LOCUS       DAVHEX010000043        20129 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVHEX010000043 DAVHEX010000000
VERSION     DAVHEX010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20129
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.043"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(63..1685)
                     /gene="groL"
                     /locus_tag="VVL45_001477"
     CDS             complement(63..1685)
                     /gene="groL"
                     /locus_tag="VVL45_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEU4187637.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(1701..1985)
                     /gene="groES"
                     /locus_tag="VVL45_001478"
     CDS             complement(1701..1985)
                     /gene="groES"
                     /locus_tag="VVL45_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEU4187638.1"
                     /translation="MLKPLGDRVVLKIEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(2141..2536)
                     /locus_tag="VVL45_001479"
     CDS             complement(2141..2536)
                     /locus_tag="VVL45_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU4187639.1"
                     /translation="MYNKVILIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            complement(2614..3375)
                     /locus_tag="VVL45_001480"
     CDS             complement(2614..3375)
                     /locus_tag="VVL45_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU4187640.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEALKA
                     EFAETFPNQTVWTFLLDVTDMTMVKTVCSDILETIGQIDILVNNAGLALGLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMVKANQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMVHKK"
     gene            complement(3417..4043)
                     /locus_tag="VVL45_001481"
     CDS             complement(3417..4043)
                     /locus_tag="VVL45_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEU4187641.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLDVERKGKVARVFLKDNGE
                     TVAWNIFEVSSLFEIAERGQVFLTDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKTVQIVAGAPNARVGLKTIVALPGAMMPKGNLIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPAKHWTA"
     gene            complement(4059..4376)
                     /locus_tag="VVL45_001482"
     CDS             complement(4059..4376)
                     /locus_tag="VVL45_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEU4187642.1"
                     /translation="MIQPASLEELASLVEKAGKKVFIFVADWCSDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKQQINDFLA
                     GLK"
     gene            complement(4373..4672)
                     /locus_tag="VVL45_001483"
     CDS             complement(4373..4672)
                     /locus_tag="VVL45_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEU4187643.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDHFSDMGEIATLYVQVYESSLESLVGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            complement(5009..5200)
                     /locus_tag="VVL45_001484"
     CDS             complement(5009..5200)
                     /locus_tag="VVL45_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HEU4187644.1"
                     /translation="MYYSVDDVVSNAFKKRMILDSFFAFNCSGTMKVSTWVYDKGEWY
                     YVSSSGSMIANDWVKDNGK"
     gene            complement(5361..6035)
                     /locus_tag="VVL45_001485"
     CDS             complement(5361..6035)
                     /locus_tag="VVL45_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187645.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLIIRGDVAFGKL
                     EIVYVK"
     gene            complement(6041..6487)
                     /locus_tag="VVL45_001486"
     CDS             complement(6041..6487)
                     /locus_tag="VVL45_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4187646.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(6601..7104)
                     /locus_tag="VVL45_001487"
     CDS             complement(6601..7104)
                     /locus_tag="VVL45_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU4187647.1"
                     /translation="MKNYQEWYRNISSRLTSCPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVALSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            complement(7101..7463)
                     /locus_tag="VVL45_001488"
     CDS             complement(7101..7463)
                     /locus_tag="VVL45_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEU4187648.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICGVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(7529..9388)
                     /locus_tag="VVL45_001489"
                     /pseudo
     CDS             complement(7529..9388)
                     /locus_tag="VVL45_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850459.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(9427..9876)
                     /locus_tag="VVL45_001490"
     CDS             complement(9427..9876)
                     /locus_tag="VVL45_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEU4187649.1"
                     /translation="MDKPMLVFKRFGHQIHLMVQKEAKRCGIEFMGGPQGQVVRFLDN
                     REKNQDLVLIKDIEQELNITKPVASNLVKRIVQNGLVELEASPVDKRAKFVRLTDKAR
                     SQMQQVKAFFERIDKQLMEDIDEDELLIFEKVLGQLQAKYQGNRRRE"
     gene            complement(10000..10716)
                     /locus_tag="VVL45_001491"
     CDS             complement(10000..10716)
                     /locus_tag="VVL45_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU4187650.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            complement(11597..13012)
                     /gene="ply"
                     /locus_tag="VVL45_001492"
     CDS             complement(11597..13012)
                     /gene="ply"
                     /locus_tag="VVL45_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HEU4187651.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            complement(13023..13355)
                     /locus_tag="VVL45_001493"
     CDS             complement(13023..13355)
                     /locus_tag="VVL45_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187652.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDYLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            complement(13426..14034)
                     /locus_tag="VVL45_001494"
     CDS             complement(13426..14034)
                     /locus_tag="VVL45_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187653.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            complement(14047..14505)
                     /locus_tag="VVL45_001495"
     CDS             complement(14047..14505)
                     /locus_tag="VVL45_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEU4187654.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            14630..15432
                     /locus_tag="VVL45_001496"
     CDS             join(14630..15080,15080..15432)
                     /locus_tag="VVL45_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEU4187655.1"
                     /translation="MNYEASKQLTDARFKRLVGVQRTTFEEILAVLKTAYQLKHAKGG
                     RKPKLNLEDLLMATLQYVREYRTYEQIAADFGIHESNLIRRSQWVEVTLVQSGVTISR
                     TPLSSEDTVMIDATEVQINRPKKELANYSGKKKCHAMKAQAIVTSRGRFVSLDITVNY
                     CHDMKLFKMSRRNIGQAGKILADSGYQGLMKIYPQAQTPRKSSKLKPLTAEDKAYNHA
                     LSKERSKVENIFAKVETFKMFSTTYRNHRKRFGLRMNLIAGIINHELGF"
     gene            complement(16170..16430)
                     /locus_tag="VVL45_001497"
     CDS             complement(16170..16430)
                     /locus_tag="VVL45_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187656.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDLTRLTASLLGDEKLSAITSSSA
                     VKADMHRFVIELEPVKSTILQNNDISLDQNEIFEILKNFLDG"
     gene            complement(16489..17040)
                     /locus_tag="VVL45_001498"
     CDS             complement(16489..17040)
                     /locus_tag="VVL45_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187657.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIDEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNPEFPSMIELFSIL
                     PEYPLKKDGREIPLHFDQDASLSALLLDEDYYNILVHEKETIQGYSVLSNCGLYSSKI
                     SSNHVSFHLQPQNSVLSSLQLAS"
     gene            complement(17030..17227)
                     /locus_tag="VVL45_001499"
     CDS             complement(17030..17227)
                     /locus_tag="VVL45_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187658.1"
                     /translation="MLEIWKYRPFVSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRI
                     EEESEALENMILQYLGEDDAS"
     gene            complement(17231..18025)
                     /locus_tag="VVL45_001500"
     CDS             complement(17231..18025)
                     /locus_tag="VVL45_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187659.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEVPKELTPSEQLTWIAFLLTKGQKVVDVDLLSQVTGLPNSTI
                     YRCLRTFKALYWLNKQNKLYTYTVSKKELFLKSVSCLFNPIKKRILLPDGDIKQIKSV
                     SNLLYGGAYALSHSTFLAETDENISYVIWQRKFNQLSLPLSQHVLK"
     gene            complement(18433..18858)
                     /locus_tag="VVL45_001501"
     CDS             complement(18433..18858)
                     /locus_tag="VVL45_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187660.1"
                     /translation="MTSRVFIDADCISVFLWVGTEHLLEKLYLGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVAKGSAEIVSIDIGTEEYALYRDLTRNHDSNKIIGKGEGASISLA
                     KKHNGILGSNNLRDVKSYVEEFSLEYMTTGDILIEAFKA"
     gene            complement(18987..19346)
                     /locus_tag="VVL45_001502"
     CDS             complement(18987..19346)
                     /locus_tag="VVL45_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187661.1"
                     /translation="MKKSSVSLILIGEGDETERKADQFASYFLIFPSSLYRMVEEIRE
                     NANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAEEIKNMDISVIETASRLGYD
                     TSLYRPLSESKKEMALG"
     gene            complement(19392..19598)
                     /locus_tag="VVL45_001503"
     CDS             complement(19392..19598)
                     /locus_tag="VVL45_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187662.1"
                     /translation="MDLSNKASNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMPLGR"
     gene            complement(19585..19746)
                     /locus_tag="VVL45_001504"
     CDS             complement(19585..19746)
                     /locus_tag="VVL45_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU4187663.1"
                     /translation="MNMINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
BASE COUNT         6029 a         4458 c         3469 g         6173 t
ORIGIN      
        1 aaaaagaagg gttagtcatt ccctcctttt gtgtttttta taagttgttt tctatagaaa
       61 gcttacatca tcccgcccat catgcttgga tccattgctg gagctggggc tactggttct
      121 ggtttattgg ctacgactgc ttctgttgtc aaaatcaagc tggctacaga tgctgcattt
      181 tgtagggctg aacgactcac tttaactgga tcaatgatac cttgatcaat catgttaacc
      241 cactcgccag ttgctgcgtt aaatcctata ccaagctcag catttttcaa acgatcgata
      301 acgatagatc cttcaaatcc tgcattgtga gcaatttgac gaacgggttc ttccaaagca
      361 cggagaacaa tattacgtcc tgttgcttca tctcctgtca attccaaggt agcaacagct
      421 ggaatcacat tggcaagagc tgttccacca cctgcaacaa taccttcttc aacagctgca
      481 cgagtagcgt tgagggcatc ttcaatgcgg agtttcattt ctttcaactc agtttcagtt
      541 gcggctccaa ccttaataac cgctacacca cctgacaatt tggccaagcg ttcttgcaat
      601 ttttcacggt caaattcaga agttgtagtt tcgatttgag acttgataac cgcaacacgg
      661 tgagaaatcg cttcaggatt tcctgcacct tctacaataa ccgtgctatc tttgtccacg
      721 gtcactctcg ctgcttgacc aagagcttca attgtcgcat ctttcaactc aagaccaagg
      781 tcttctgtga taactgttcc gcctgttaag atggcgatat cttcaagcat ggctttgcga
      841 cggtcaccaa aaccaggtgc cttgactgct actacgttga aggttccacg aatcttgttc
      901 aaaacaagag ttggaagagc ctcgccatcc acatcatccg caataatcaa gagtggacga
      961 ttgctttgga gaatgctttc caaaagtggc aagatttctt ggatattgga aattttcttg
     1021 tctgtaatca aaatgtacgg attttcaagg tcagccacca ttttttcgct atctgtcacc
     1081 atgtactgtg aaaggtaacc acggtcaaac tgcattcctt ctacgacttc aagctctgtt
     1141 tccataccac gtgactcttc gatggtgatg acaccgtctt tgccaacttt ttccattgct
     1201 tcagagatgt actcaccaac tttttcagaa cgagaagata cggctgcaac ttgagcgata
     1261 gcttctttat tggcaacagg gatggcgttg tttttcaaag cttcaactgc tgcggcaact
     1321 gctgtttcaa tcccacgacg aataccgatt ggatttgcac ctgctgtgac gtttttgatt
     1381 ccttcacgga cgatagcttg ggtcaagact gttgcagtcg tagttccgtc acctgcgata
     1441 tcattggttt tagaagctac ttctgatact aacttagcac ccatattttc aaaatggtct
     1501 tccaattcga tttctttggc aatggtcaca ccgtcattgg taatcaaggg tgaaccgaat
     1561 gacttttcaa gaacgacatt gcgacctttt ggtcccaagg ttactttaac agtgtctgca
     1621 aggatatcga caccacgaac catggctgaa cgggcatctg atgaaaattt aatttctttt
     1681 gacatactta ctttctcctt ctattcctca atgattgcca aaatgttagc ttcgcctacg
     1741 atgatgtact tttcatcgcc atctttgaca tcaagacctg cgtgggcttc aactaagaca
     1801 cgatctccag ttttaacact tggagcaacc aagtcaccgt tcaaggtacg aacaccttgt
     1861 ccagtagcca caacttgagc tgttttggtt ttttcttggg ctgagcctgc aaggacaaag
     1921 cctccaacag tttgttcttt ttcttctatt tttaagacca cacggtcccc taatggtttc
     1981 aacatctgat ttcctccata atgagataga tattattagc actctttgaa cttgagtgct
     2041 aattcatagt tctattgtat cacttggtca gaaataatca agaaaaaagt ctgactttct
     2101 caagataaaa agcctgagac caactcagac tttttaattc ttaaaatggc aattcttcct
     2161 cttccaagac caaatctgcc aaatcttggc ctgcattatt ttcacgcata gcacgttggg
     2221 cacgactttc caagagttgg aatcctgtga caagtacttc ggtcacgtag ttcatttggc
     2281 catttttctc aaagcgacgg gtacgcaatt ctccatcaac ggaaatgaga ctacctttgg
     2341 ttgcgtagct tgccaaagtt tctgctagtc tgccccatag gaccatattg acaaaatcag
     2401 cttcacgttc accgttttgg tctttgtaac gacggttcac agcgatagtt gctcgcgcta
     2461 ccgacttgtc attgttggtt ttgtgcaatt ctggtgtaga cgttaaacgt ccaatcaaga
     2521 taactttatt atacatattt tcttcctcct acttatctat tcgtaggaaa tcaaaaaaag
     2581 ttacagaaat ttgtaacttt tcgagaaaat tttttatttt ttatgaacca tgaaacctgt
     2641 cgcctgttga ttggccataa tggtcatatc tgtaatctga acacgacgag gttgactagt
     2701 cacatagact actgtatctg caatatcctg agcttgcaaa gcttctattc cttggtaaac
     2761 ggacgcagct cgttctttat caccatgaaa acgcactgta gaaaaatctg tttcgacaat
     2821 tccaggctga atggtcgtca ccttgatatc cgttgcgatg gtatcaattc gcagtccatc
     2881 tgaaaaggtc ttaactgccg ccttggtggc tgagtaaaca gctgcaccag cataggcata
     2941 aattcctgcg gttgacccca tattgataat atgaccttga ttggctttta ccattgctgg
     3001 caagaaacag cgagtgactg ccatcaaacc tttgacattg gtatccaaca tggtcagcat
     3061 atccaactct tcatagtctt gatagggagc taagccaaga gccagtcctg cgttattgac
     3121 caggatgtca atctgaccta tcgtttctaa aatatcagag cagacagtct ttaccattgt
     3181 catatccgtg acatctagga gaaaagtcca aactgtttga tttggaaaag tttctgcaaa
     3241 ctccgcctta agagcttcta gtctgtctat ccgtcgtcct gttagaacga catcctcacc
     3301 ctgctccaga taagcacgcg caatcgcttc accgattcct gatgtcgctc ctgtaatcac
     3361 aacatttttt gccatcttat ttccttctag ctggtctatc agatattaac aacttcttag
     3421 gcagtccagt gtttcgctgg gtcgaacggt gttccgacaa cttggtcttc tgataattca
     3481 agcaccccac gtttttgtgg agcatttggc agatgcaatt cacgaggact gcacatcata
     3541 ccaaaactct tttcaccacg aagttcacct gggaaaatga gattcccttt tggcatcata
     3601 gctccaggaa gcgcgacaat ggttttcaac cccacacgcg cattgggagc tcctgcaacg
     3661 atttgtacag tcttatcact tgcgactgca acttggcaga tgttgaggtg gtcactatct
     3721 ggatgggcta ccatctcaac aatttcacct acaacaaact taggttcctt atcattaaca
     3781 atttcttctg taaaaccttc cgcctgcaac tcttggttca aacgagcgac ttgctcatct
     3841 gtcaaaaaga cttgaccgcg ctctgcaatt tcaaataaac ttgaaacttc gaaaatattc
     3901 caagccactg tttccccatt atctttgaga aaaacacggg ctaccttgcc tttgcgctcc
     3961 acatccagtt tggcatctcc gctatttttc acgatgacca taaggacatc accgacatgt
     4021 tctttattat atgtaaaaat cattgtttcc tttttctcct atttcagtcc tgctaaaaag
     4081 tcattgattt gttgcttgct tttacggtcg cgattgacaa aacgaccgat ttccttgtcc
     4141 ttttctagaa caacaaggct aggaattccg taaacatccc agagtttggc caaatccata
     4201 tactgatctc ggtccattcg aataaaggtg aactctggat tggtctcctc aatctctggt
     4261 aaggcaggat aaatataacg acaatcgcta caccagtctg ccacaaaaat gaagaccttc
     4321 ttgcccgctt tttccactaa agatgctaat tcttctaaac ttgctggctg tatcataaga
     4381 cttcctcctc atagactagg tcttcatttt catagacaaa ggtataatga cggccatcct
     4441 caaaaatgac gccaccaacc aagctctcca gactgctttc gtaaacttga acataaaggg
     4501 tcgcaatttc ccccatgtcg gaaaaatggt ctcgcacaat ctctgtcaac tcttcctgag
     4561 tcttcatgag cttacggtca tctgcaactt ttttcgtagc aagagcaagg cttccgatac
     4621 ctagcagagc caagcctgcc atccacattt ttttagcttt cataccattc attttaacac
     4681 aaaaaaggct tcaggacaaa tgaggaagca gcagaaaagc aagtaaaaag cctcttcctt
     4741 taaggaaaag gacttcttat actcaatgaa aatcaaagac caaactagga agctagccgc
     4801 aggctgctca aagcactgct ttgaggttgt agatagaact gacgaagtca gctcaaaaca
     4861 ctgttttgag gttgtggatg aagctgacgt ggtttgaaga gattttcgaa gagtattatt
     4921 cttattgcca ggcacctaag ttgccaacgt agtaactatc aggtgtgtag gtattgcgaa
     4981 gcatcttacc tgatgaagcc agataatact acttgccatt gtctttgacc caatcattcg
     5041 caatcatgga accagaagaa cttacataat accattctcc cttgtcataa acccaagtac
     5101 tgactttcat ggttcctgag caattaaagg caaaaaaact gtccaatatc attcgttttt
     5161 taaaagcatt tgacactaca tcatctacgc tgtagtacat agatacatca aacgtaaaag
     5221 ccaaaatatc gaaaaatata caacttttaa aggcagcatt tcatttttta aagtctaaca
     5281 accatagaac tgaaatcaga gcaaggcatc aatttcctac tcttagtacg tctattttga
     5341 tggttgaagt gaagattttt ttatttaacg tagacaattt ccaacttccc aaaagccaca
     5401 tctccacgga taatcaaggt tttgctggtt ggggctacag gagcgtcagc cttagctgca
     5461 ccaaaggagg tttctacttt caaatctaca cgccagtgtt gtggaacata gacggttgca
     5521 ttcccgaagg ccacttcaat atttaaagtt gcaaaatcac ctagcatctc tgcattatca
     5581 tagtagattt tagcatcccc aaaagcgact tccacttggt catctacgag atcttgatct
     5641 tgcttataga aggtcccgct accaaaagcg acttccttat ccgtgacgac tgtttttttc
     5701 ccattgtacc accatttttt ttcattccag aacttacttg aatgcgtcag ataaccaaca
     5761 ccaagtacca ccaagatgct agcccaaata agagaatgat tggtaactgg taacaagtca
     5821 taagcgtaat ttgcaatgat gagcgccagt aaacctgtaa aaactgccga agtgagatga
     5881 cgtctaagga tggactcaat ggacttataa gcaaaaaaaa caatacctag taaaggccat
     5941 attttaccat ccaaagaagg aattccaaaa ttcccttgca gcaagatcca agctgctaaa
     6001 accaataaaa caataccaaa tgctttcttt ttcatgtttt ttacctcatg tttcttagat
     6061 tttcttttag gagggattgg taatgccgtg agacatgaac ctccttgtgc gtctgataaa
     6121 aggaaatggt gcccgttcct gaaaaggact tgtccactga gtaaatctga cggatgtttg
     6181 cgatcgttga cttggaaact cgattaaaat agcgaggcaa gatagactcc aactcataga
     6241 gcttgaggcg aacctcataa gcttccttct ggttatgagc gtagatcttg ctcccttctg
     6301 tttcaaagaa gagaatttct gacaggtcta gataatattc acctgtcccc ttgtaaaaag
     6361 tcaacctcgg agcctttgac tcttgcaaga ggcgttgcaa gtctgcaatt tcatctgtca
     6421 aagtgggggt cttgatgaca atttcagtct cctctaaatt gccgtcaatc tcaattcgta
     6481 acttcatcac atctcctttc tgactctact atatcaaagc taagttagga atacaagggc
     6541 aaaaaagcaa gtggtcaatt tgaagcagta agtggttgta ggactagctt aacttacatg
     6601 tcagaatagg aagaaaagga atccccacac caaaccacaa gcatagccaa ccaagacatc
     6661 cttgggataa tgaacacccc ctaaaaccct caccaacccc agcaaagctg aaaagatcaa
     6721 gcatactaag ccaataggta gactagcatg cagacaggcc atggagatga tagttgccga
     6781 aaagacatgg cgactgggca tcgaccgtcc cgaactatcc ttatcaagaa ggggacagat
     6841 atcccaagtt tcataaggtc tcgggtgatt gattttctta cgaaacaggg acaaggccac
     6901 aaaacctgat gcaggaataa gcacataaac cccaacttgc tgaccaagtc ctagttgcag
     6961 gtaagtggtg actagcaaga tcagatagac caggggcatg gcgactgtca tcaaacgatt
     7021 gaaactgcgt aataggaata aaagaattgg acaactagtt agtctggagc tgatatttcg
     7081 ataccattct tgataatttt tcatttatgc ttctcatact tttctagaac ttgttgagat
     7141 gttttcccct tggcaagcca atccactagc ttgtccaagc gacgtatttc ctgcatcaga
     7201 ggatcatcaa tttcctcaat acggacaccg caaatcttcc ctgtaattgc aattcgttca
     7261 gggacataat atggtgcttg tctaaaaaaa tctccgtaag taacatccct atccagacaa
     7321 gcaagaacat cactcacttc ataccctgtc aaccaactgg tcacttggtg aactgactct
     7381 gcctttccac cctttcgctc aacttttgca atcaaggcta agtagacagc agcaaatttc
     7441 atattgtata atttctgact catacttctc tctttctatg tgtactagta taaataagaa
     7501 aaagaaggcc gtcaagcctt cttttgattt attcttctgc ttcatcttct gtaaattgac
     7561 tattgtacaa gtcagcgtag aagccacctt gcgccatcag ttcctcatag ttgccttgct
     7621 cgatgatatt tccatctttc atgaccaaga tcaagtctgc atttcggatg gttgacaagc
     7681 ggtgggcaat gacaaaggat gtgcgtcctt ccatcaaacg gtccatggct ttttggatca
     7741 attcctctgt ccgtgtgtca acagaagaag tcgcctcatc caaaatcaaa agcggtgcat
     7801 ccttaagaag ggcacgagca atagtcaata gttgtttttg tcttacagac aaggtcacgg
     7861 tgtcatccaa gatggtatca tagccatctg gcaaggtcat aataaagtgg tgaattccca
     7921 cagccttact agcttccatc attcgttcat cactaatccc tatttgatta tagatgagat
     7981 tgtctcgaat agttccttca aagagccagg tatcctgcaa gaccattgaa aaggcatcat
     8041 gcacttctga acgcgtcata gccttggtat ccacaccatc aatgcgaata cttcccttat
     8101 caatctcata gaatttcatc aaaagattga caatggttgt cttaccagcc ccagtcggcc
     8161 caacaatggc aaccttttga ccagcatgag ctgtcgcaga gaagtcatag tcttgaacat
     8221 tgacaccgtc caccagaatt tctcctgctg acacgtcgta gaaacgtgga atcagattga
     8281 ccagagttga tttaccagaa cctgttgacc caataaaggc cactgtttga ccagtttctg
     8341 ctttaaagct aacatgttca ataactgcct ccgaatttgc cgcatagcgg aaggtcacat
     8401 ccttaaactc gacctgacct ttgaagtttt catcagtcag ctgcacttga acagggtttt
     8461 ggatagaaga atgcaaatct aaaacttgat taatccgctt agcagagacc atagttcggg
     8521 gaagaacgat gaagagtgct cccatgagaa ggaagcccat gacaacctac atggcataag
     8581 acatgaaaac aatcatgtca ctaaagagag gcagacgcgc tatcggagca gcgtcgttaa
     8641 tcacataggc cccaatccag taaatcgcca cactcaaacc acttgaaatc cccatcatga
     8701 taggattcaa aatagccata agacggttga caaacaaatt caaacgggtc aattcatcat
     8761 ttactgctgc aaatttttca ttttgataat cctctgcatt gtaggcacga acgacacgaa
     8821 tacctgttaa actctcacga gtgatactgt tcagtttatc tgtcagcccc tgaatcaagg
     8881 actgttttgg aaaggctagc gtcatcaaaa cggtcgtcat caggacgttg ataatcactg
     8941 ccacaagtac ggcccagagc cagtattctg aatgacctaa aatcttccca atagcccaga
     9001 tagccataat tgaaccacgc gttaccactt gcaagcccat agtaatcaac atttgaactt
     9061 gagtaatgtc attggtagta cgcgtcaaga ggctaggaat tgaaaatttc ttaatctctg
     9121 tctgcgagta atccaaaact cggttaaaaa tatcacttct cagcctacta gtataagaag
     9181 ccgccactcg ggatgcaaaa aatccaactg caactacgga caagaaggca agaaaggaca
     9241 ttcccatcat catgcttgcc gactgccaca actcatctaa attagtttct tgactaccta
     9301 gcaaatccgt aattttcgag atataggtcg gcacttccaa ctctagatag accgaaaagc
     9361 aagtaaagag aatggctagt aaaatcatcc cccattcttt tctactaatt cttttggcta
     9421 atttctttat tctctcctcc tattcccttg atattttgcc tgtagttgac cgagaacctt
     9481 ctcaaaaatc agtaattcat cttcatcaat gtcttccatc aactgcttgt ctatgcgttc
     9541 aaaaaaagcc ttaacctgtt gcatctgaga acgtgctttg tccgtcagac gaacaaactt
     9601 agcccgctta tcaacaggac tcgcctccaa ttccaccaaa ccattttgca ctatacgctt
     9661 aaccagatta ctagcaacag gcttggtaat attgagttcc tgctcgatat ctttaatcaa
     9721 gaccaagtct tggtttttct cgcgattatc caaaaaacgc acaacctgac cttgcggccc
     9781 acccataaat tcaatgccgc aacgtttggc ttccttttgc accatcaggt gaatttgatg
     9841 accaaaacgc ttaaagacta acatcggttt atccataatc tcccccttct aaataaaaat
     9901 agttctctgg agaataatta aatttctatg agaactattt tcttgattaa aaaaatccca
     9961 agtgattttc tcacttagga tcatgttcta taggttaaat taaaacccat ctacgttcgt
    10021 ataaatcttt tggacgtctt cgtcgtcttc aagaacgctg taaagttttt caaaggtttc
    10081 aaggtcttcg cctgacaatt ccacttctga ctgaggaatc atttccaatt cagtcacttg
    10141 gaattcttca ataccagact cacggagggc aacgatagcc ttgtgaaggt cagttggcgc
    10201 tgtgtaaact gtgattgtac cttcttgtgc ttctacgtca tccacatcca catccgcttc
    10261 gagcaattgc tcaaagactg cgtccgcatc ttcacctcca aatacaataa cacctttgtt
    10321 gtcaaagagg taagaaacag aacctgaagc gcccatgttt ccgccgtttt taccaaaggc
    10381 tgcacggaca ttggctgctg tacggttgac gttagaagtc aaagtatcca caattagcat
    10441 agagccattt ggcccaaaac cttcgtaacg tccttctgta aaggtttcgt ctgtgtttcc
    10501 tttggcttta tcaatcgctt tatcgataat gtgttttggc acttgggctt gtttagcacg
    10561 gtcgataacg aatttcaaag ctgagtttga ttctggatct ggatcacctt ttttagctgc
    10621 tacatagatt tctacaccaa attttgcata tactttagag ttagctccat ctttagccgt
    10681 tttcttggct acgatattgg cccatttacg tcccattagg aatctccttt tttcacattt
    10741 taatctttct tattataaca caagtttttt tgattttcac tagaggaaat ggattttatt
    10801 agcaaatcaa gctaggatag cactttacct gctaagatgg tcttgccttt ctatctttat
    10861 caacaggcac tcatccacat tcaaaaaaca aactagacca ttatctgcaa atagaaagtt
    10921 tcagccaagt ttgacaaagt cagctcaaat tactgtttga agtttgtaga tataagcgac
    10981 aaaaacaatc atactgcacc ttttgttgac agtctactcc agacatatca tagttcaagt
    11041 aaatactttg aaattcaaca gttcttatag gcgctattgt attctaagaa atcaatagaa
    11101 gagtttctaa gcaaacctct aatactcaat aaaaatcaaa gagcaaacta gaaagctagc
    11161 ctcaggttgc tcaaaacact gttttgaggt tgcggatggg gctgacatgg tttgaagaga
    11221 ttttcgaaga gtataattta cgtgttccca agatggagaa gttagactag tacactggca
    11281 cttctaaaac attgctagca attgatttgt tcatatttaa tttcattttt tccataaatg
    11341 ggtattagat ataaacagca aaatatttcc gatacgtgtc gttcttgaat ttccaatcat
    11401 ctaaaacaag taaaggataa tcaatcccct gtatatcaag gaattggcta ccctttttac
    11461 ttttttacac attctgtttg atagattcat tttaacatca cgagcatact ccaatggaaa
    11521 tcgctaggca agagataaac tttcagatat ccgcagagag atcatcgcct ctttttgtcg
    11581 caagcattct cctctcctag tcattttcta ccttatcttc tacctgagga tagagagttg
    11641 ttccccaaat agaaatcgtc cgcttacgca ctagtggcaa atcggttttt tcataaaccg
    11701 tacgccacca ttcccaggca agcccggtac actctctaat tttgacagag agattacgaa
    11761 cattcccttt taaaggaata ctagtggtaa agtgagccgt taaatcctgc ccatttctgt
    11821 cccaagcctt aggagtcaag acttccttac cttgatgatc ataggataat tcattccaag
    11881 taatataata ttgggcaaca taggcaccac tatgatccag cagtaaatct ccgtttctgt
    11941 aagctgtaac cttagtctca acatagtctg tactattttg aaaggtcgca actacattgt
    12001 cacgtaaaaa agaagttgta taggaaatcg gcaagcctgg atgatctgct gtaaagcgac
    12061 tgccttcttg aatcaagtcc tctaccatat ccaccttgcc tgttacaact cgggcacccg
    12121 aacttgggtc gccccctaaa ataaccgcct tcacttctgt attgtccaaa atctgtttcc
    12181 actctgtctg aggagctacc ttgactcctt ttatcaaagc ttcaaaagca gcctctactt
    12241 catcactctt actcgtggtt tccaacttga gatagacttg gcgcccataa gcaacactcg
    12301 aaatatagac caaaggacgc tctgcagaaa ttcctctctg ttttaaatcc tctaccgtta
    12361 cagtatcttg aaacacatct cctggatttt taacagcatc tacgctgact gtataataaa
    12421 tctgcttaaa attaacaatc tgaatctgct tttcgcctga atggacagag ttaaaatcaa
    12481 tatcaagaga attccctgtc ttttcaaagt cagaaccaaa cttgaccttg agttgttcca
    12541 tgctgtgagc cgtgattttt tcatactgca ttctagctgg gacattattg acctgaccat
    12601 aatcttgatg ccacttagcc aacaaatcgt ttaccgctcc gcgaacactt gaattgctgg
    12661 ggtcttccac ttggagaaag ctatcgctac ttgccaaacc aggcaaatca atactataag
    12721 tcatcggagc acgatcgacc gcaagaagag tgggattatt ctctaacaag gtctcatcca
    12781 ctacgagaag tgctccagga tagaggcgac tgtcgttggt agctgttaca gaaatatcac
    12841 ttgtatttgt cgacaagctc cgcttctttc tttcgataac aacaaactca tcgggtagct
    12901 gattaccctc tttgatgaaa cgattttcaa tactttctcc ctgatgggtc aagagtttct
    12961 ttttatcgta attcatagct agtataaagt catttactgc tttatttgcc atcttctacc
    13021 tcctaataag ttcctggatt gagttgcata aactcagact tgttcagcga aatcagccgt
    13081 ggttggacta agtaatccaa aatttcctcg tacaattctt ctgagacatt gcgtcgccgt
    13141 ctggctaaat aagaagtcgg aatgaccgta ttatccaaca taaatacctt atctaagtca
    13201 atcaaggttg gtcttgtaaa aggattacga gctagatccg gctcttctat cataaagttc
    13261 ttgaccaaac gtctggtcaa gagagctggt ttgaaggtct gatttttaac caactctttg
    13321 tttttagtca tgctgttgtc aatacagata tacatatgat tcttcacagc caaatcgcta
    13381 ctaatagtcg gaaaaggcaa ataaagagct acaacatctc ctctcttaat caagcaagag
    13441 cacccccttt tctcctaatg taacatagac aggattgacc aagtcttctg attgactcag
    13501 aatttccaaa gtttgagttt ggcgcgctgt caatttagta gcatcttgtc tcttcaatac
    13561 aaaatgcttg tcgccaataa ccttgacaat ataatccttc tccaaagctg actggtaaat
    13621 ccacatcaga tgttgtctgt cctgagaact caagagagaa ggattttcaa gcctcccgat
    13681 agtctgataa aaatcaaaaa caggagctaa ctcctgccaa tctgattggc tagttgtcaa
    13741 ggctagaaaa agggctttgc gagctgatac ttcttggtta gccttgagag ttactttccc
    13801 ctccaagttt tttagaaatc gggaaactcc agaaagcaaa tttttctcta actgcgagaa
    13861 ataaaaacct ttcgttccca gacataagtc tttcatgtcg ctttctctag caaataagag
    13921 ctcaaacatt tgatagtaaa agaaaaatat ctggcactgg gtcgcgctca tcttttcctt
    13981 atcggcttct ttttttaacc agagcaaggg cgacaggtag ctggattgag acatttcctc
    14041 tacctcctac tcttttttaa ctggagcatc tgcactagct gccacttctt ttgactggat
    14101 actttcccac tggttaatct cctctgagat aagaccttcg catgtcttga caaatagggc
    14161 aaaagccttg gtctttcctg catatttctc cgtttggcat tgatagagga atttttcttt
    14221 ctccaggagt tgcgcagttt tttggtaaga aatccaattt tcctttgcat tatacaaatt
    14281 gataatcccc tcacacagca agccgagact ggataaggca accgaaatca aacggtagcg
    14341 atcacctggc ataggaatag cacaaaagac agctatgagg aaacctgcca cgatttctgt
    14401 tatttttaat accttatagc gcctacgatg ttgaacgctt ttctttaaaa aatgagctat
    14461 ctgtacgtct aatcgctctg tcaggtacat ttcttctggc gtcatattcg taactccttt
    14521 catttacttt gataatcata gtataacata tttgaatatc aaagtatagt aaaaactgga
    14581 ctataactta ttagacttcc tgcgaaacaa aatatggtat agtagttcta tgaattatga
    14641 agcaagtaaa caactaactg atgcacgatt taagcgtctt gttggtgttc agcgcacgac
    14701 ttttgaagag atattagctg tattaaaaac agcttatcaa cttaaacacg caaaaggtgg
    14761 acgaaaacct aaattaaacc tagaagacct tcttatggcc actcttcaat atgtgcgaga
    14821 atatcgaact tatgaacaaa ttgcggctga ttttggtatt cacgaaagca acttaatccg
    14881 tcggagccaa tgggttgaag taactcttgt tcaaagtggt gttacgattt caagaactcc
    14941 tctcagttct gaggacacgg taatgattga tgcgacggaa gtacaaatca atcgccctaa
    15001 aaaagaatta gcgaattatt ctggtaaaaa gaaatgccac gctatgaagg ctcaagcgat
    15061 tgtcacaagt cgagggagat tgtttctttg gatatcactg tgaactattg tcatgatatg
    15121 aagttgttca aaatgagtcg cagaaatatc ggacaagctg gtaaaatctt ggctgacagt
    15181 ggttatcaag ggctcatgaa gatatatcct caagcacaaa ctccacgtaa atccagcaaa
    15241 ctcaagccgc taacagctga agataaagcc tataaccatg cgctatctaa ggagagaagc
    15301 aaggttgaga acatctttgc caaagtagaa acgtttaaaa tgttttcaac aacctatcga
    15361 aatcatcgta aacgctttgg attacgaatg aatttgattg ctggtattat caatcatgaa
    15421 ctaggattct agttttgcag gaagtctatt atatatgcac agacatatga aatctttttg
    15481 agatagcagt tatgatatat ttatccattc acaacatctt ctgtaccaat actacaatat
    15541 tggtacattt tgattacaca atcgtttcaa aaaatatgat ggaagcctga gtattttata
    15601 gtaactccct tcctatcaac tagatcaacc agttgagtgt tgaatgttga aacaccctag
    15661 cctcagcaaa ggactatgag cagcatttcc tagctaatag gacgctccaa atcacctatt
    15721 ctaaatccat cactagcttg gtcagtaaga tacagactgc aaggcaagac tgacgtggtt
    15781 gatgagattt tggaagagta ttacaatcat agattcacca gtagacttga ccgcagattt
    15841 aactatagta gattgaaact agaatagtac acttctgctt ctaaaacatt gttagaaatc
    15901 gatttgactg tcctgatcaa tttgtcctgt tctcgtttca ttctactata ctaaaaaacc
    15961 acctgaaaca aatcaagtga ctagaggata aaaacgaaga ctgggcaaaa agttttgaaa
    16021 agcaaaaaac gcataatatc aggtcttgaa aaaccttgat actatacgtt tgattgtggg
    16081 aagattgact tcattttctc ctgaaattga agttttttct agtttccaga tatagacgaa
    16141 ttcaatatag ccatctcgct acaattattt taaccatcga gaaaattttt cagaatttca
    16201 aaaatttcat tttgatccaa tgaaatgtca ttattttgaa gaatagttga cttcacaggc
    16261 tctaattcta tcacaaagcg gtgcatgtct gcttttaccg cactacttga tgttatagcc
    16321 gataactttt catctcctag caaggaagct gtcaaacggg taaggtcatt caaatgcttt
    16381 ttaatggact tacttaaacc ttgagcacct gtggcagagc gctccctcat atccagccaa
    16441 gctttagcct taaaaataat taatactcaa tgaaaatcaa agagcaaact aggaagctag
    16501 ctgcaggctg ctcaaaacac tgttttgagg ttgtagatgg aagctgacgt ggtttgaaga
    16561 gattttcgaa gagtataaac cacaattact caataccgaa tacccctgaa tggtttcttt
    16621 ttcatgcacc aatatattat aataatcttc atccaataat aaggctgata aactagcatc
    16681 ttggtcaaaa tgtaagggaa tttctcgacc gtccttcttt aatggatatt ctggtaagat
    16741 actaaatagt tcaatcatag aaggaaactc aggattagtt gttgtaaatc gaaaaagctg
    16801 cgctttctca tctttctgac ttccttgata ctctcccaat tctaaaaaat gattcaaggt
    16861 agtataaaat tccttatttt ttacttcatc aatgatgacc atatcataat cttttgttgt
    16921 gcgactttta aatccttgcg aatccaatac gatagaggta gcagttcccc caatcagaac
    16981 ataatagttc tgaaaatccg caaacatttc ttgaaaaata actttcgtat tagctggcat
    17041 catcttctcc cagatactgt aatatcatat tttctagtgc ttcactctct tcctctatac
    17101 gtgggtcatc atcatctttt aaggtcaaat aaagagaaat cggatctaca aattgtttat
    17161 catgattatt tttaaaatca ttccaaaact cagatacaaa aggacgatat ttccatatct
    17221 ctagcatctt tcattttaaa acatgctgag aaagtggcaa ggataactga ttgaattttc
    17281 tctgccatat gacatagcta atattttcat ccgtttcagc taaaaaagtt gaatgcgaca
    17341 aagcataagc accaccatat agaaggttag aaacagattt tatctgcttt atatcgccat
    17401 ctggcaataa aatccgtttt ttgatgggat taaataaaca tgacacggat tttaagaata
    17461 attctttctt tgacaccgta tatgtgtaaa gcttattttg cttgtttaac caatataaag
    17521 ctttaaaagt cctcaaacac ctataaattg ttgagtttgg aagtccagtg acttgtgaaa
    17581 gcaaatcaac atctactact ttttgacctt ttgtcaataa aaaggcaatc cacgttaatt
    17641 gttcgctagg tgttaattcc ttagggactt cagtatcatt cgcattgagt actagtccca
    17701 atggagggaa gaagaggttt cccttaaagt ctacaaacgg aactctagct tgaagtaatt
    17761 gctttttttc actgtctgat aacttcgaaa acaccaaaac aacatccata ttggcttttt
    17821 cacccatagt gcgagcctga gtaacaaatg aactcaaact cccccttctc ttttctttaa
    17881 tcaataaaaa tgattcctta tgaaaagtca tttgagtata ctcaaacctt tttacaaaac
    17941 tgattggtaa agtcaaagtc agttcctcaa tactgacatc aacataaaag gattgaaaaa
    18001 ttgacattat ctttttgaca ttcatgtttt ttccttattc tatcattttt attatatttt
    18061 atcattcggt ataataaaat ataagttttt tgataaaata accaaaaact actcgctatc
    18121 ccctctcaca ttaccctaca tatcgtttga catgcgactg atagttcagg aaaacttcaa
    18181 ggagaacttt tctctcatcc actatgcagg acttactatt tcacttctac tcctataggc
    18241 tcaatttgac acttttcttt ttcgaattgc tcatatgcct cctgaatagt gagttcgatt
    18301 tatccaaatt tatttttgtc tattttgatg aatacttcca cgaagatagt ctgaaaatga
    18361 atttgcacca attttccttc tctttttaag catattattc cagatatgat tgccctctta
    18421 ttcagtaata aattacgctt taaacgcttc aatcagtata tctcctgttg tcatatattc
    18481 taaagaaaat tcttctacat atgatttaac atctcttagg ttattacttc ctaatatccc
    18541 attatgcttt ttcgctaagg aaatagatgc cccttctccc ttaccaataa tcttgttact
    18601 atcatgattt cttgttaaat ctctatataa tgcgtattct tcagttccaa tgtctatgct
    18661 cacaatctca gctgaaccct tagctaccaa ctgatctatc ctagatttta aatggggaat
    18721 tgtaggtata ttgatttcat catacacctc ttgtggaata acaattttac ccaaatagag
    18781 cttttctaaa agatgttcag tgccaaccca taaaaatact gaaatacaat ctgcatcaat
    18841 aaatacacga ctagtcaaac gacaactccc ccctcttcat ctagcccata tacaatatca
    18901 tatctgaaag catctagtaa cagttcctca tacttccctt gcgaaattct gttattttct
    18961 aaaagttgtt cagttgaatt aatatattat cctaatgcca tttctttttt actttctgac
    19021 aaaggacgat ataaacttgt atcatagcct aatcttgaag ctgtctctat aacactaata
    19081 tccatatttt taatttcttc tgcatcaagg tatccatcat tcctcaatct atataacata
    19141 gctttatgac tgataccata aaactgaccc aattttataa tatcttctac ttcaagatga
    19201 gttctattgg cattttctct gatttcctca accatcctat acagtgaaga tgggaaaatt
    19261 aaaaaataag aagcaaactg atccgctttt ctttcagttt catctccttc accaatcaag
    19321 ataagactga ctgaactctt cttcacctca tcataataaa gatgatacag tccatgtgct
    19381 aaagaaaatc tttaccttcc taatggcatg tctgaattga ctgcaatgag actgaactga
    19441 gttcctttat aacagactcc gctaatattc tttccgagtc cataaaatac cagcgtcaaa
    19501 ttttctatct tttgtaccaa tttaaaaata tctatcggcg attcaccatc agctcccaac
    19561 ttttttctaa gatttgaagc tttattactt aaatccattc gtgatattcc tttcaccttt
    19621 ttccattttg gcaatcatgc tttgattcaa agacaaatac tcagcaatct tatcttgagt
    19681 aagatttgaa gatattctaa gttcttttat tctttttcca acatcacata catttatcat
    19741 attcatacac ctctcataaa aagaatagca cactcttgtc ataatttttt aaataaaaaa
    19801 attatgacaa aacaaggaag caatttattg atgctgctta aaaatctaaa attgatgagt
    19861 tcctatcaaa aatcgtatct tcaacctcaa aacagtactt aaagctatcc gactcggttt
    19921 acattgtcaa atttagattt tatttgagca taactttcta gtttgctttt tgatttttgt
    19981 ttaatatagt agcaaaattg aaggaaatat ctccacaaga aaacgcatac tattaagctt
    20041 tttcaagacc taataatatg cgctgttctg atttgaaaga cattccatta ttattttact
    20101 gtaatcaagc catctggctc tactgtgaa
//
LOCUS       DAVHEX010000044        20012 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVHEX010000044 DAVHEX010000000
VERSION     DAVHEX010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20012
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.044"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(<1..617)
                     /locus_tag="VVL45_001505"
     CDS             complement(<1..617)
                     /locus_tag="VVL45_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEU4187664.1"
                     /translation="MDFEYFYNREAERFNFLKVPEILVDREEFRGLSAEAIILYSILL
                     KRTGMSFKNNWIDKEGRVFIYFTVEEIMKRRNISKPTAIKTLDELDVKKGIGLIERVR
                     LGLGKPNIIYVKDFMSIFQVKENDLQKSKNLTSEVKDFNLRSKENELQEVKNLDSNYI
                     ENNKSKYSKREYSFGENGLGTFQNVFLAAEDISDLQIIMNSQLEN"
     gene            complement(726..1244)
                     /locus_tag="VVL45_001506"
     CDS             complement(726..1244)
                     /locus_tag="VVL45_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEU4187665.1"
                     /translation="MRTYENKEELKAEIEKTFEKYISEFDNIPENLKDKRVDEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
     gene            complement(1471..2340)
                     /gene="rsmI"
                     /locus_tag="VVL45_001507"
     CDS             complement(1471..2340)
                     /gene="rsmI"
                     /locus_tag="VVL45_001507"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEU4187666.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFGL
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETPLKGECLLIVEGASQGVEEKDEEDLFVEIQTRIQQGVKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            complement(2343..2660)
                     /gene="yabA"
                     /locus_tag="VVL45_001508"
     CDS             complement(2343..2660)
                     /gene="yabA"
                     /locus_tag="VVL45_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEU4187667.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            complement(2700..3590)
                     /locus_tag="VVL45_001509"
     CDS             complement(2700..3590)
                     /locus_tag="VVL45_001509"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEU4187668.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFESLEMAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQGEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            complement(3587..4225)
                     /locus_tag="VVL45_001510"
     CDS             complement(3587..4225)
                     /locus_tag="VVL45_001510"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEU4187669.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVEVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKAVLFDGMGLAK"
     gene            complement(4341..5138)
                     /gene="proC"
                     /locus_tag="VVL45_001511"
     CDS             complement(4341..5138)
                     /gene="proC"
                     /locus_tag="VVL45_001511"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEU4187670.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQTRTSDEILLANRSQAKVDAFIAD
                     FGGQASSNEEMFAEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSELFCQLLSKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLSRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMEAVHQAYKRTQELGK"
     gene            complement(5142..6404)
                     /locus_tag="VVL45_001512"
     CDS             complement(5142..6404)
                     /locus_tag="VVL45_001512"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEU4187671.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAASFLPRLEQ
                     VLVAERKEAGLEPIQFRLDSKASQFVSGQAAQAQDFDTEFLDYILAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVAGDGQIRE"
     gene            complement(6414..7544)
                     /locus_tag="VVL45_001513"
     CDS             complement(6414..7544)
                     /locus_tag="VVL45_001513"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEU4187672.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSA
                     QVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQYGKSLLLSGIVEAEGVFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            complement(7624..9507)
                     /gene="cbpE"
                     /locus_tag="VVL45_001514"
                     /gene_synonym="pce"
     CDS             complement(7624..9507)
                     /gene="cbpE"
                     /locus_tag="VVL45_001514"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HEU4187673.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDSRITNSERLWDNLYGYDKVLQT
                     AAETGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRKDGFVNISTSYKPIPS
                     FQAGWHKSSYGNWWYQAPDSTGEYAVGWNEIKGEWYYFNQTGILLQNQWKKWNNRWFY
                     LTDSGVAAKNWKKIAGIWYYFNKENQMEIGWVQDKEQWYYLDVDGSMKTGWLQYMGQW
                     YYFAPSGEMKMGWVKDKETWYYMDSTGVMKTGEIEVAGQHYYLEDSGAMKQGWHKKAN
                     DWYFYKTDGSRAVGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVPIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSVNKSE
                     SEQ"
     gene            complement(9510..10397)
                     /locus_tag="VVL45_001515"
     CDS             complement(9510..10397)
                     /locus_tag="VVL45_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU4187674.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLALAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            complement(10387..10848)
                     /locus_tag="VVL45_001516"
     CDS             complement(10387..10848)
                     /locus_tag="VVL45_001516"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEU4187675.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            complement(10845..11753)
                     /locus_tag="VVL45_001517"
     CDS             complement(10845..11753)
                     /locus_tag="VVL45_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU4187676.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            11746..11943
                     /locus_tag="VVL45_001518"
     CDS             11746..11943
                     /locus_tag="VVL45_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU4187677.1"
                     /translation="MFIFYPQLLYLFIIPYFRSFPKRKKEHPILLNYLHYLPFSRQSY
                     FQNQFKKISGYTPLQYRKEKR"
     gene            complement(11995..12666)
                     /locus_tag="VVL45_001519"
     CDS             complement(11995..12666)
                     /locus_tag="VVL45_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU4187678.1"
                     /translation="MKKRAIQILLALSLIFYKSIWFWRVFNHLAKLYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISFNETVEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(12720..12941)
                     /locus_tag="VVL45_001520"
     CDS             complement(12720..12941)
                     /locus_tag="VVL45_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HEU4187679.1"
                     /translation="MILMTKNINLTNEELELIQGGADPYGKNPNGRYDWEIEPVLTLP
                     VHGFCPRGTYDLGYIGGGNHLCKGSAARF"
     gene            13213..14022
                     /locus_tag="VVL45_001521"
     CDS             13213..14022
                     /locus_tag="VVL45_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU4187680.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLELDLQNEYVIVNNGCSTHQTSDWSLVDWKELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKLRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
     gene            complement(14203..15078)
                     /gene="aguB"
                     /locus_tag="VVL45_001522"
     CDS             complement(14203..15078)
                     /gene="aguB"
                     /locus_tag="VVL45_001522"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEU4187681.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(15088..16173)
                     /gene="aguA"
                     /locus_tag="VVL45_001523"
     CDS             complement(15088..16173)
                     /gene="aguA"
                     /locus_tag="VVL45_001523"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEU4187682.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALERPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVA
                     AFVGPAELVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEKRYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            complement(16170..17297)
                     /gene="nspC"
                     /locus_tag="VVL45_001524"
     CDS             complement(16170..17297)
                     /gene="nspC"
                     /locus_tag="VVL45_001524"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEU4187683.1"
                     /translation="MKLEQVPTPTYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDADLEELLGITDHIVF
                     NSERQLRKHGARCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFEAQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            complement(17297..18556)
                     /locus_tag="VVL45_001525"
     CDS             complement(17297..18556)
                     /locus_tag="VVL45_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEU4187684.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVITLIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVEKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            complement(18553..19413)
                     /gene="speE"
                     /locus_tag="VVL45_001526"
     CDS             complement(18553..19413)
                     /gene="speE"
                     /locus_tag="VVL45_001526"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEU4187685.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGAFMLPKYVE
                     DILEEEEGKK"
     gene            19668..>20012
                     /locus_tag="VVL45_001527"
     CDS             19668..>20012
                     /locus_tag="VVL45_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEU4187686.1"
                     /translation="MLYVGIDIAKNKHDVTALNVPGKTVLKPLTFSNNKAGFELLDLS
                     IRQINQDCLIALEDTGHYAFNLLNFLHEQGYKVYTYNPLLIKEFAKSLSLRKTKTDKK
                     DAHGIALKLLSDP"
BASE COUNT         5800 a         4628 c         3550 g         6034 t
ORIGIN      
        1 taattctcaa gctgtgagtt cattatgatt tgtaaatccg atatatcttc agcagctaaa
       61 aacacatttt gaaatgttcc aagtccgttt tcaccaaaac tatattctct cttactatac
      121 ttactcttat tattctctat atagttagag tcaaggttct taacctcttg aagttcattt
      181 tctttacttc tgaggttaaa atcttttact tctgaagtta agttttttga cttctgtaag
      241 tcattttctt ttacctgaaa tatactcata aagtctttaa cataaatgat gttcggctta
      301 ccaagtccaa gccttactct ttcgatcagt cctattcctt tttttacatc aagctcatct
      361 aatgttttta tggcagttgg ctttgagata tttcttcttt tcataatttc ttcgacagta
      421 aaatagataa atactctgcc ttccttgtct atccagttat tcttaaatga cattcctgtc
      481 cgtttaagaa gtatggaata aaggataatt gcttctgctg ataagccccg aaattcttct
      541 ctatcaacta atatctccgg tacttttaag aagttaaatc tttccgcttc tctgttataa
      601 aaatattcaa agtccatgca agatcacctc ccttcttaaa aatttgcaaa gaaaaagacg
      661 atagtttttc ttctatcgcc tttggtaacc atttttatga aattttttaa agttaaaaat
      721 ataatttata atactatctt cttccatttt ctgattttag ttctgaaagt tccaaaaggt
      781 gcaaccgtat ttacatgaat aaacttatac acttcccaag tcgctgtttt agtcgcttca
      841 tcagcccact ttctcatatg cggttcaaat aattcttcct cactcaacga atcaatcatt
      901 gcagagatag aattaatatt ttcatttaat tttgctttca actcttgcag agataaatga
      961 gcgtaggtat ctgtgaacca ctgatataat tcaccaagtt gattccattt aaatttatcc
     1021 gatggtgttt ttacttgaag cccctttctt tcatcttctt cccatttaag aaccaagttg
     1081 gtccagccaa cctgataagc aaggttttct gctggagttc tatcaacttc atcaactctc
     1141 ttatctttta aattttctgg aatattatca aattctgaaa tatatttctc aaatgttttc
     1201 tctatctcag cttttagttc ttctttattt tcgtatgttc gcaaaaaatc accttctttc
     1261 tgatttatca atcctttgat ttaaaatgac cttatttttc tatttatacc ttctaggaac
     1321 gctataactg ctgctccaac catcggtatt ccgtatgggc ttaataaaaa acttaatacc
     1381 aaagcttcta taccaagtga tacatcttta tattatcatc actaattaag ttaaacagaa
     1441 acagacagaa ttattacatc ctgtctccct ttattgtttt tcttcccagt cgtggtaggc
     1501 agcgtagagc tgacttttat tccactggta aatcttagcg acttccttga tagcttggtt
     1561 tttcttcaca ccttgctgga tgcgggtttg aatttctacg aacaagtctt cctcgtcctt
     1621 ttcctccaca ccctgactgg caccctcaac aatgagaaga cattcgccct tgagtggcgt
     1681 ttcagcaatg ctttctaata actcagagat agtacctcgt tggtattctt catagatttt
     1741 ggtcaattcc ctgaccaaga caactgagcg gtcaccgtag acttctaaca tattttccaa
     1801 cgtgtctgct acacgatgag gtgattcata aaaaatctgt gtttcaggat aatctttttt
     1861 caagccaaaa aattgcttct gctgacctga ttttctcggt aaaaaaccgt aaaagatatg
     1921 tggctgtggc gctaaaccac tggcaatcaa ggcagaaatt cctgcagagg cacctggaac
     1981 tgtcacaact gcaatttctt cctcaatagc tgccttaact aaatcatgac cagggtctga
     2041 aatgctaggc aaaccggcat cagagacctg agcaatactt tgccctgctt tcaagaaacc
     2101 aatcaaatca ggaatttttt ccttggcatt gtgctcatga aaactgatct gcttggtgga
     2161 aatgtcaaaa tgcttgagca aaagacctgt attgcgcgta tcctcagcag caatccaatc
     2221 tacttctttc aaggtctgga tagctcgaaa ggtcatatca tctagattgc caatcggcgt
     2281 tgccactaga tacagcttgc catagggaga ctgcccctta aaactttttt gaatctgcat
     2341 gcctactccc tgtatagcaa ctcgtcacaa aacatacatt cctcgtcctg ctctcgacgt
     2401 tgtccataaa aatcattaca tacgtgaaat ccatcacggt aaatgcgacg gacactttca
     2461 cgaacatgct tggccttgac aggagcatct gcttccacct cacccaagcg ttctcgcaac
     2521 ttactatttt ccaagcgaag agctgtattt tcctctacca ggctcttgag atttttcttg
     2581 atggcttcca catcggctaa ggttaccaat aattgttggg aaaaatcatc cagcgcgtca
     2641 aataattctt ttttgtccat aaaacagccc tttcctttct ttatcattca tttttgagtt
     2701 tatatttctt tcaagaccag atattccatg gcattttgaa agctgacatt agcttgccac
     2761 atttttctag cttctagtaa atcttgtaga atcactctta ctcttacctg caagaggtcc
     2821 tgcccacaga gaacttcaag aatccgtaaa acctgatcct gtttttcctt atcatctgcc
     2881 aagttggcta atttggcaac ctgtagataa ctttcttttt tcttagctac taaccaagtc
     2941 agcaggcgtt cactttcatc gaccaaggtc caaaaacttg cctgattagc caacttttct
     3001 gcttcagctc gcgattgact aaacttagct aaaagagtcg cttttttctt aacaagtccc
     3061 atttgttcta agagtaagat aagtttttct tcttgctttt taaagtggaa gatctgagtc
     3121 cgacttcgga ttgtcggtaa catcttttcc tcatcgctag tcaagaagaa aatataaact
     3181 tcactctggg gttcttcgat gaccttgagc agagaattgg ctgcgttggg atgcatttta
     3241 tccgcttgct cgatgataaa gacctgttgc tggctttcaa tccctgcttg agaaaactga
     3301 cccaccaatt ctcgaatgcg ttccgtctta atgacctgat taactggttt aatcaaggtg
     3361 acatcgggaa attctccctg ttcaatcagc ttgcaacttc ggcatttctc acatggtaag
     3421 acgccaactt tatccgtaca aaagaggctc ttagctaaaa attgcgccat ttccaagctt
     3481 tcaaagaaac ctgaaaagag ataggcgtga ttgagctggt cttgttctaa gatacggaca
     3541 aaacggtcaa actgagctgg ttgccaagcc tttagttgat cttgtttcat ttggccaagc
     3601 ccattccgtc aaacaagaca gccttggtag tttccacaac ttgctccaaa gggagactag
     3661 catcaatctt gacaatgcga tttccctctt tatccagaag agaaaggtag ccttgacgaa
     3721 cttttttatg caagtccaac ccttccaaat ccaaacgatt aacctcgcgg tcactattag
     3781 cagcaatacg agccagccct tcttccacct cgatgtcaaa atagagtgtc aaatcgggtt
     3841 tgaggccatc tgtcgcaaac tgattgagcc agtcaatggc ttcaatatct aagccacgac
     3901 caaatccctg ataggcaaca gaactatcga taaaacgatc catgatgacc aacttgccag
     3961 cttcaagggc tggaagaact ttttccacca aatgctgtct gcgactggca atatagagaa
     4021 gtagctctgt tttagcatcc atctgagtat gacttggatc caaaatcact tcccgaatct
     4081 tctccccaat caagactccg ccaggttcac gggtcgtcaa cacctctact cctttttcct
     4141 ctaaaattgg tagcagagcc tctaaaacac tggtcttgcc tgctccctct ggtccctcaa
     4201 gagagactaa aaatcctttt gacatgtcta actcatttct tttttactac ttctattcta
     4261 tcaaaaaaat aggggtttgt gacaatcttt ttgtgtcttc tcatttcagc caccataaaa
     4321 gaggcagtca aagctacctc ttatttacct agttcttgtg ttcgtttata ggcttgatga
     4381 actgcctcca tgactgtgcc tcgaaaagca tgcgcttcta ggcttgctac gccagcaata
     4441 gtcgaaccgc ctggactaca aacttgatct ttcaaaactc caggatgttg ctggctttct
     4501 aggaccaatt gcccagctcc taccacagtt tgggctgcca ttttcaaggc tgtttctcgt
     4561 gataatcccg tctgaacacc tgcatctgcc aaggcttcaa taaagagata gacaaaggct
     4621 ggtccacagc ctgcaagacc tgtcgctgca ttgattaagc tttctcctaa ttcaaccaag
     4681 agaccagcct tggataaaag ctgacaaaag agctcactgt cctcagccct gcaattagga
     4741 gacaaggcat aactaatcac tccttgcccg atagaagcag gggtattagg catcatacga
     4801 ataattcggt gttgacttgg gataagactt gctagttttt ctaaggtcaa tccagctgcc
     4861 atcgaaatca aaagaagact ttctcttttt tcaaggatgg tctggtattg agaaagcagt
     4921 tcagaaaact gagcaggctt aactcctaga aaaatcacat ctgcttctgc aaacatttct
     4981 tcattgctgg aagcctgacc accaaagtct gcaatgaaag catctacctt agcttgacta
     5041 cgattggcaa ggagaatctc atctgacgtc ctagtctgca agacagattt tgccaagcta
     5101 gcacccatat tccccaaacc gataaatcca atcttcatct cttactccct tatctgccca
     5161 tcaccagcaa ccacatactt gtagctggtc aactctttca agcccattgg accacgcgcg
     5221 tgcaatttct gagtagaaat ccccatttca caaccaagac caaattgtcc tccatctgtg
     5281 aaacgagttg aggcattaac atacaccgct gcagagtcca cttgatctgt aaagtatgct
     5341 gcagcttcag cattttccgt cacaatggca tccgaatgat gggtactgtg ggattcaata
     5401 tgcgcaaccg cttcttctaa actgctcaca accttaacag ctagaatata gtctaaaaac
     5461 tcggtatcaa agtcttgtgc ttgagcagct tgacctgaaa caaactggct tgctttgcta
     5521 tctaggcgga attgaattgg ttccagtcca gcttccttac gctctgcaac caacacttgc
     5581 tccaagcgag gaaggaagct tgctgccttg ttttcatgaa ccagcagaac ctccatggca
     5641 ttacaaacag aaggacgact ggttttagca ttgttgatga tagacagcgc cttgtcttcg
     5701 tctgcatcct tatccacata gacatggaca atcccagtcc ctgtctcgat aacaggtaca
     5761 atcgcattct caaccactgc attgatcaag ccagctcctc cacgaggaat gagaaggtct
     5821 agatagccct tggccttcat catagcataa ctactttcac ggctagtatc ctccaccagt
     5881 tgaatcacat ttggatgaat agtagtcgtc tccaagccct tcttcaaggc tgtgacaatg
     5941 gcatgggttg tttgataggc atccttacca ctacgaagaa caaccgcatt tccactctta
     6001 agagtcaaag cagccgcatc agacgtcaca tttggacggc tttcatagat aataccgatg
     6061 acacccatag ctacacgttt ttttgtgata accaaaccat tttcaagctg acttgtttct
     6121 aaaacttcac cgattggatc tggtaaggca accacttcac gaattcctct tgccatcgct
     6181 tctatacgat ctgcatccaa ataaagacga tccaacatca catctgagat tttcccctta
     6241 gccgctgcca tatcgagggc attagccgct aaaatttcct cagtagcagc cactaagtga
     6301 tcagccatgg ctagcaaggc ttggtttttc acttcttcac tagctgtgtt aatcgatttt
     6361 ttaacagcct gtacctgttc aaattgttct tgtctactca ccatagttta cctctaaaat
     6421 tctgtaaaaa gtagttggat ttcaggagta atggaaatcc agtcgtcacg gtaaatcaag
     6481 acacccttgg ctttttgaga acgcaacata tcctccaaag cagatgctcc aaattgcacg
     6541 cgtccttttc caagtgattt tccactttcc ttgtcaaata ctgtcacgat atcaccgtaa
     6601 gaaaagactc cttctgcttc aacgatacca gataagagaa gactctttcc atattgagag
     6661 agagcttccg cagccccttt atcaacccaa atagaacctt gactctgagc atagaaggca
     6721 agccattgtt tctgggtacg aagccccttc tcttgagcaa caaagtaaga accatcctcg
     6781 gtctcctctg ccgcctcaat catggaatct gatttcaagg atgagcagat ataaacagga
     6841 actcctgatt ccgtcgcgat agttgcagcc ttgattttgg ttaacatacc cccagttccg
     6901 tttgacgaac cagctccacc agccatatca ataatctcac gattgatggt ctcgattctc
     6961 tccaagcgtt tggctcttgg atctgaatta ggatttccag tatagagacc gtccacatct
     7021 gtcaagaaaa ctaaaaggtc tgcttggacc atcgccgcta cttgagcact tagagtgtca
     7081 ttgtccccaa ccttgagctc atcaataacg acactatcat tctcattgat gataggaatt
     7141 gccccacggt tgagcaaaac cgacaaagcc tgatgggcat ttttataacg acgcttatcc
     7201 acaaagtcat cttgggtcag caagatttgt gcagaaacga tttgacgcaa gagaagattg
     7261 gttgtatatt cttccaacaa aagcccctgc cctaccgctg ctgaagcctg tttatcagca
     7321 atcttagtcg gacgcttttt aaatcctaag gctccaaaac cagccgcaat ggcacctgaa
     7381 gacaccaaaa tcaactcatg accagcctcg tgcagcatag ccaactgctg ggtaatatcc
     7441 tttaccttac tacgtgataa acttccatcc tcatttgtca gagaagaagt acccacctta
     7501 aagacaatcc gtttgtattt catagtctca ctccgattat tcatatttat ccattataac
     7561 atgaataaca ggaaatagaa aagagttgat aggaaaaaga aggccctaaa accttctttt
     7621 ctactactgt tctgattccg atttgtttac actcgtttca gagctttgtg aagttgagga
     7681 tgtcaaatct tgactagttg aagaatttgt aacactttca tgttgactcg tttctttatt
     7741 ttcaagatcc tttttcacta cttctttgga ttcttttatt tctgaatgac ttgtagtttt
     7801 aattgtagca gatttctcga tcggaacatc cactaaccag gcaccacttg aatccacagt
     7861 atagccttct ggtgtcttac cattcacaag taattgacca ttttctttca agaagtacca
     7921 tttatccttg tccttgatcc aacccacagc tcgtgaaccg tctgtcttgt agaaatacca
     7981 atcatttgcc tttttatgcc agccttgctt catagctcct gaatcttcca gataataatg
     8041 ttgaccagca acttctatct cacctgtctt catgacacca gtagaatcca tatagtacca
     8101 ggtttcttta tcttttaccc agcccatttt catttcccct gatggagcaa agtaatacca
     8161 ttgccccata tattgaagcc atcctgtctt catagaacca tcaacatcca aataatacca
     8221 ctgctcttta tcttgaaccc aaccaatttc catctggttt tctttgttaa aataatacca
     8281 gattccagcg attttcttcc aatttttagc agcaacacca gagtctgtca aatagaacca
     8341 acgattgttc cattttttcc attgattctg taataagata cccgtttggt taaagtaata
     8401 ccattcaccc ttaatttcat tccaaccgac agcatattct cctgtagaat caggcgcttg
     8461 ataccaccag tttccatatg aactcttatg ccaaccagct tgaaaacttg gaatcggctt
     8521 gtaggatgtt gaaatattga caaaaccgtc ttttcgaata tcaaaaactg tcgcatcata
     8581 gtctttgctg gctgcgttga ttctctcaat tcctcgttct ttgagccaat taacatactc
     8641 actatcaaca ccatttttcc aaggtagact atccgaagtt tgaacaatca aactcggact
     8701 caaattttta atgaaatcct tggtatttga tttgttggta tcatggtgat gattaaactt
     8761 catcaaatca acttttccaa tgagaggacc atacttgtct tctgctccat gaacattatc
     8821 taaatcgccc ccaaggtaaa ttttcttgcc attgactttc accacgctaa tcaaggaatt
     8881 ggaattgtca tcccaaattt tctttaattc acccgatgaa tcagtttcat tttcataatt
     8941 atagagctga atatccatgt ccccaaactg aaaatgagca tccccttgtg ttatattttg
     9001 aataactgaa acacctgttt ctgcagcagt ctgtaaaacc ttatcatagc catacagatt
     9061 atcccataga cgttcagaat tagtaatacg actatcacta tatttcttaa gatagactcg
     9121 gtcaactgga taggtagaca gtaattcatc aacatttcca atatgatcac tgtgggtatg
     9181 ggtcaccaaa ataaaatcaa gtttttggac acccaattcc ttcaaacgac gaaagacacg
     9241 gtctgttaga acatgcttat aagacgtttc aattccttct ctccatggat agcgagaatc
     9301 acttccatct gggaaatcat aatcttctcc tgtatccacc atggcaaaat gtccattgct
     9361 ttcaagaata atcgcatcac tgccaccttc ttgaacattg ataaagtgga ttttatttcc
     9421 tgaactttct tgagcctgaa cgttcccata ccatgacaaa cctaaaaaag cgcctacaag
     9481 tgctaaacta gttaatttct ttttcattct tactttctca atctctccaa ggtttcctta
     9541 aaaatctctg ggatatctgc tttaaattcc aaggtcttac ctgttctcgg atgagtaaaa
     9601 cctaaagtct tggcatgaag aaattgtcca tgtcctttca aagtcttgcg aggaccatag
     9661 acctcatcac cagcgactgg atggccgata taagccatgt ggacacggat ttgatgagtg
     9721 cgccctgtct ccagttgcaa ctctactaag ctataatcgc caaagcgttc caagacgtga
     9781 aaacgcgtca ctgcaggctt ccctttagca gttacagcct gtttcttacg gtctttttca
     9841 ctccggccaa tcggcgcttc aattacacca cgatcattag gcagatttcc atgaacaatc
     9901 gcccaatatt tgcggagaga ctttttatcc ttgagttctt gggcaagtgc tagatgcgca
     9961 tcatcgtttt tagcaatcat gagaagacct gacgtatcct tatcaatacg gtgaacaatc
    10021 cctggacgca aaaccccatt gatacccgac aagtccttaa tatgatacat gagggcattt
    10081 actagggttc cactggtatg accagcactc gggtgcacaa ccattccctg aggtttgtta
    10141 acgacagcca catcctcatc ttggtagact atttctagcg gaagatcctc agccacatac
    10201 tctaatacct ctggttctgg cacatggtaa gtgacgacat caccctcttg gactgtgtat
    10261 ttagctttct tgacttgacc attgaccaag acctggcctg atttaatttg ttcattcgcg
    10321 agactacgtg ataattctga caaatctgac aaagccttat ccaaacgcag accaccagtt
    10381 tcaattttaa tttccattta tttcctcttt tagcattgca atcaataaaa taatcactcc
    10441 aaccgtcaga tagctatctg ccacattgaa aattgcaaag ttgataaagt caaggtggaa
    10501 catatccaca acaaagccct gactgaccct gtcaataaag tttccaagac cacccgcgat
    10561 tattagagtc aaacccaaga ccatccagaa tgagtcctcc atgtgtttat gtaaatacca
    10621 aatggcacct atcacgacaa ccagagtaat gacagcgaat aacagctgct gatcttgtaa
    10681 gatagaaaag gctgcacctc gattttgcag gtaggtcaag ctaacgaaat tggggatcca
    10741 ggagcgcact tcacccagtg gaatctgctg gacgatatag gatttgacca actgatccag
    10801 cccaatcaaa agcagtacaa tgactgccac tattgctctt tttttcatga tttcctcttt
    10861 tgatcaaaat attcttgcat gacttctacg aagagagtcc cagcttgact aagctccact
    10921 tcttcacgtt taacatagac catgcggtta tctaggttat ccttgagacg aataactgta
    10981 atgccattaa cactgtcact atctaaaaat ccagaacctg tcgcataggc gtccgtccgc
    11041 tccaaaatac cattcaaggt ggcacggtct gtcacattaa acatctgtga gctagcgctg
    11101 gtatcaacaa agttctctga ataataaagg tactcgtctt tctcttgagt gaaacgaacc
    11161 gttggtaaat ccgctaaatc ctccatgact aattcctctt tctgggctaa aggatgaccc
    11221 tcacagagat aaatatgggt atggaaagga atcaattcga tgacctccag acctaacttt
    11281 tcaacccgtt gcataatccc ctttttattt tgattgttga ggtagataat cccaatctca
    11341 ctatgccctt gcgccacttc atctaatatt tgaacagtag ttgattcaaa aatacggaag
    11401 ttcttatagt caggatagcg ctctgaaaag gccgtaatag ttggtggtaa gaagtcatag
    11461 tgctggctag caacggaaaa ttcatctttt tcttcttcag gattggcata ctgattttga
    11521 aaaatatcaa atcctttaac caattcttgc gctttttcat aaaattccat cccacgacgg
    11581 gtcaagaaag tccctgagct ggtccgacgg aaaatcttaa agcccaactc tttttccaaa
    11641 tcacgaacag aaatagacag actcggctga ctaacataca tcttttcagc agcttcacga
    11701 aaagtaccac tattggcaat agccacaaca tagcgtaatt gttgaatgtt catcttctac
    11761 ccccaacttc tttatctttt cattatacca tattttagaa gttttccaaa aaggaaaaaa
    11821 gaacatccta ttcttcttaa ctatcttcac tatctgcctt tctcacgcca atcttatttt
    11881 caaaatcaat tcaaaaaaat aagtggctac acaccacttc agtatcggaa agaaaaacgt
    11941 tgactatttg tgaaaaaaga aaatgccgga aaattccgac atttttttag taagctaact
    12001 tcctgaaaat aggagtaacc aaacatccca gaaggcagtg atattgatga gaatatgaac
    12061 tagtattgga tagtagagat ttttagtcat gcgatacaat agagctaaaa taagacctcc
    12121 actagcataa atgaaaaaag caagaggagt taaagcaaaa ttgatatgaa tataaccgaa
    12181 aataatagca gaaagcaaaa catctccgta ccaaggtgag tttttgaaaa aggttgtcat
    12241 aagcacacct cgataaatca attcctcagc aataggggcg atgaagcaaa cgatgagcaa
    12301 gaaataaggg aactcctgtc tccccatcat ttctaccgtt tcattaaaag aaatctgatt
    12361 tgaagacagg gatatgaaat acgaaaagag gaagtcagac atatatgaaa tgatgtagcc
    12421 cagtaaaagg tagatgaagt acctcagctg ccacttgaaa tgaaaaattt tctttcctgc
    12481 aaaaaccagt agatagatga ccgctaagaa aagaactccg ctctccatca aaagcagaat
    12541 ctgaaaaaat tcacgacttg ctggtagata gagctttgcg aggtgattga aaactctcca
    12601 aaaccaaatt gatttgtaaa aaattaagga caatgctagt aaaatttgaa tagcccgttt
    12661 tttcatatta aattctctgc tctctccact tgtttcttca tattcctaat aaattttact
    12721 taaaatctcg cagcacttcc tttgcaaaga tgattgcctc ctccaatata tcctaaatca
    12781 taggtgcctc tgggacaaaa tccatgaacc ggcagagtta atactggttc tatttcccaa
    12841 tcgtacctac cattaggatt tttaccatat ggatctgctc caccttgtat cagctctaat
    12901 tcttcatttg ttagatttat attttttgtc attaaaatca tgatatattt cctccattat
    12961 tcttcagatt agttgagtaa tctaatactc atacctactt acaaaaaaac tattatatta
    13021 agttggtttt ttaatttgtc tagttgcaac attgacaaac ataatatagc atatctttgc
    13081 gaaatatcct cttcaaatca tgaattgtca tcaaaacatc ttaaactata aaatcaatta
    13141 gtctcaagct ttctatcaat ttcttctcca aaatatgcta taataatagc aaaagataaa
    13201 gaaggaagac ctatgattaa actactagcc ttggatatgg acggcaccct cctcaatgaa
    13261 gccaaggaaa tcccacaagc tcacattact gctattcaca aagccattga aaaaggtgtc
    13321 aaactggttc tctgtacggg tcgccctctt ttcggtgtcc tcccctacta caaaaaactg
    13381 gagcttgacc tccagaatga gtatgtgatt gttaacaacg gttgttcaac tcaccagacc
    13441 agtgattgga gtctagttga ctggaaagaa ctgagtccag ctgacatcga atacctctat
    13501 gaccttgctg aaaagagtga tgttcagttg acactttttg acgagtcaca ttattttgtt
    13561 ctcggtggca aacccaatca agttattgaa aatgatgcta aactagtatt ttcagacctg
    13621 actgaaattt ctcttgaaga agcgactagt ggaaagttac ggatgttcca aggtatgttt
    13681 ttaggaacaa aagaacaaac agacgatttt gagcagcgtt ttgctgaaga gctttgccaa
    13741 cgattcagtg gagttcgttc gcagcctgtc atttatgaag caatgccgct tggaacgaca
    13801 aaggctactg ctctttcacg actagctgag attttgaaga ttgattcctc agagattatg
    13861 gccatgggcg atgctaataa cgatatcgaa atgctccagt ttgcagggct tgggattgca
    13921 atgggaaatg ccagcgatta tgtcaaatct cttgcggatg ccgttacctc aagcaacgaa
    13981 gaagacggcg ttgcgcgtgc tattgagaaa tatattctat aaaaaagaaa agcaaataga
    14041 cagaagttac tgctatttgc ttttttgcta atattttaaa atacggacta atcaatagac
    14101 aagaatagcg aatcataaaa atcgatttac tagatgccat agtatcttat agttgctaat
    14161 aagaagttag tatagcagaa tgaatctctc atttctccca cactaatctg taatttgtct
    14221 atacatttct ggtcttctat ctcgaaacaa gccccagttt aggcgttcac ttgctccctt
    14281 gtctaggtca taagtagcta acagaacagc ttcttcttgt ctttcagctc gttctagaat
    14341 agctcctgtt tcatccgtca taaaggagga accgtagaag tcaagactgg aactctgtcc
    14401 gccattttcc tcactaggag taacctcctc taaaccataa cgattggctg cgatgactgg
    14461 aacaatattc gctgctgcgt gcccttgcat agtacgttgc cagtgaccac aactatctgt
    14521 atccaaaatt ggctctgaac cgatagctgt aggataaaag agcaattcag caccattcaa
    14581 tgcaagacag cgcgctgttt cagggaacca ttgatcccaa cagataccga taccaatctt
    14641 agcatagcga gtattccaga ccttgaaacc agtgttacca ggcgtgaaat agaatttttc
    14701 ttgataataa tggtcatctg gtatatgggt ctttcgataa acgcccagca cttccccatc
    14761 tgcatcaatg acggcaatag agttatacaa gacattacca tctttttcat agaaactgat
    14821 tggtaaaaca acttgtagtt ccttagcaat caccttaaaa tgctgaatgg cagtattttc
    14881 cgctacagat tgggcatact ggtagtagtc atactgacgt tcctgacaga aatagggatg
    14941 ttcaaacaac tcgggcaaga gaataatttg ggctccttgc tcagcagcct gacgtactaa
    15001 acgctctgcg gtttggatat ttgttgccac atccttagcg cattgcatct gaatggttgc
    15061 aactcttaca tttctcatct ttttctccta ttctggaatt tgttgggtga tacagtggat
    15121 attgccacca cctaagagaa tatctctggc tggtattccg acaactttac ggtctgggaa
    15181 acacttgctg aggatatcta aggccacttg gtcgtttaca tcctcaaact gtggaaccaa
    15241 gacagccttg ttggcgatat aaaagtttac gtaggaagct gctagtcgtt cacctgcgta
    15301 tcgcttttct tctccttctt catagatgta gcctggcaaa tcttcttctg tcacaacttg
    15361 tcgaactgca gggataggca atttatgaat ggtgaagtga caaccttttg catctgtttc
    15421 ctgttctaag agttcgagat ctgcttttga catggcatac tggggatcat tttcgtcatc
    15481 tgtccaagcc aaaacaagct cagcaggacc aacaaaggca gcaacattat cgacgtgttc
    15541 attggtttca tcctgataaa taccataagg aagccaaata actttttcag caccaagact
    15601 ttctaataat gtgttttcaa tctcctcttt agtcaagtta ggattgcgac caggactaag
    15661 caagcaactt tcagttacga gaatagttcc ttgaccatcg ctatggattg cgcctccttc
    15721 cagtacaaaa ggtttagcat catagacagg cctttccaag gcctcagcaa aacgactggc
    15781 tacttggtca tcctcttcat aatcttgata aagaccatca taggtgcctc cccaagcatt
    15841 gaaggcccaa tccacggcta atttcttacc tttatcattg acgagaatgg ttgggccagt
    15901 atcacgcgcc caggcatcat tggtgggaat gtctaaataa acaaccttgt ccccaagata
    15961 ggattgggct tcagatagat aggcctgctc caccaaaaga tagactcttt ccccttctgc
    16021 tatggtctcg atgatctgag taaatgctct tttagcagcc tttccttgaa aaggccatga
    16081 tcctggtcga gtcggccata tcatgagggt accatgatgg ggttcgtact ctgctggcat
    16141 gtgatagcct aatttttttg gactgtccat catgataatc tccctttaaa gtcttgatag
    16201 ccaaaagctt tgagtaagct acagtctccc tgttcgtcca tgagatagag acttggcaat
    16261 ccaataccat taaaggtatt atttttgaca aaagaataaa tggccatgtc ttgaaaataa
    16321 agtctgtctc cgatttggac tggattttca aaactataat caccaatcac atcgccagtc
    16381 agacaggtat tagaagaaag tctgtaggta tgggcttttt cctgtgcctc aaagccattt
    16441 ctcaaaggtg gacgataggg catctcaagt acatcaggca tatggcaggt cgcagaggcg
    16501 tctaaaacca agatttccat accgttttct acaatatcta atacctcagt tgctaaataa
    16561 cccgcattaa gcgcaatggc ttcaccaggc tcgatataga tttcaagatt gtaagttttt
    16621 cggatacgct tgatttctga aatcagcaaa tccacatcgt aaccttctct tgtaatatga
    16681 tgaccaccac ccatattgag ccattttacc tcatgtaagt agggaccaaa ctgttcttct
    16741 actgctttca aagttgtttg taaatcatct gctccctgct cgcaaagggt atgaaaatga
    16801 agtccgtcaa ccaaatctag caaatcactc ggaatcttgt ctatagtaac tccaaagcga
    16861 gaacctggtg cacaagggtc atagagcgcg tgatctcctt gagttgaaca ctgagggttg
    16921 aggcgcaaac caacactgac accagcctct cgacaacgag ccccatgctt acgcaactgt
    16981 ctctctgagt taaagacgat atggtctgtt atcccaagca attcctccaa gtctgcatcc
    17041 ttgaaagcag gcgcaaatac atggacttca ccaggaaatt cttcccttgc caatttggcc
    17101 tcatagagtc cactagctgt cgtacctgat agatactggc taatcaaggg ataagttttg
    17161 tagagggaat atgccttctg ggcaagcaag accttgcaac cggcctcttc ttgtacatat
    17221 tgtagaatgc ggcaattagc ttctaacttg gccaagtcaa taacataggt tggtgttggt
    17281 acttgttcta acttcattag tccaccattt gtggattttc aaccacaacc catggcaaac
    17341 catactcatt caaagcttcc atgaatggat ctggatctaa ctcctcaagg ttatacactc
    17401 cagcttgttt ccaagttccg ttcatgacta attttgtccc aatcatggct ggaacgcctg
    17461 tcgtgtatga aattgcttgt gacccgactt ctgcatagca ctcttgatgg tcgcaaacat
    17521 tgtagatgta gatagtcttt tcaacgccat ctttgacacc tgtaaagata catccaatat
    17581 tggttttacc tacagtacgt gggccaaggc tagcaggatc tggaagcaag gctttcaaaa
    17641 attgaattgg aacaatttct tggccgttaa agttaatggt atccgtacga aggagtccaa
    17701 cattttcaag acatttcatg tgcgtcaagt aagattgacc aaaagtcata aagaaacgaa
    17761 tgcgtttgac acctggaatg ttcttggcca atgattcgat ttcttcatgg tgaaggagat
    17821 acatatcttt ttgtccaact tgagggaaat catactcacg cttgatagac atagcttcga
    17881 cttcgaccca tttcccatct tcccagtaag aacctggcgc agaaacctca cggagattaa
    17941 tttctggatt aaagttggtt gcaaatggat aaccgtggtc accgccatta cagtctaaaa
    18001 tgtcgatata atggatttca tcaaaatagt gtttgagggc ataagctgaa aagacactag
    18061 ttacacctgg gtcaaaacca gaaccaagaa gagcagtcaa gcctgcttct ttgaatttct
    18121 cttgataagc ccactgccat gagtagtcaa agtaggctgt aaaaccaagt tccttacaac
    18181 gtttttcgta gatagcacgc cactcagggt cttctgtgtc ttctgcttcg tagttggctg
    18241 tatcgatata gtgaacacct gttgccaaac aagcatccat aatggttaaa tcttgataag
    18301 gcagagctac attcaaaaca gcttctggtt tgtagctttc aatcaaggta atgacctctt
    18361 ctaccttgtc agcatcaagt gctgcagttt caattttagt acttgttttg ccttctagct
    18421 tcgctttcaa gtcatcgcat tttgacttgg tacggctagc aatcataatc tctgtaaacg
    18481 tttcgctatc ttgacaaatc tttgaaatag caacttgggc aacgccccca caaccaataa
    18541 ctagtaaacg actcattttt ttccttcctc ttcttctaaa atgtcctcaa catacttggg
    18601 caacataaag gctcccacgt gtaagtttgc agtgtagtat tctgtgaaaa gctggcgttt
    18661 tttccagcct tccttgtcaa aatctttgac agggtggtat tttttcgatg caaatccaaa
    18721 caaccaatag ccagctgggc tagttggaat atgggcctga taaacccgac tgattggaaa
    18781 ggcttgattg accttgcggt gcatgcttcg gcaggccgac tcatcctcgt caaagaaggg
    18841 actcccatgc tggtaaatca tgatgccgtc ttccttcaga gctcgataac tattgccgta
    18901 gaattccttg gtaaagagtc cttccgtatg gccaaatgga tctgtcgcat cgttgatgat
    18961 aatatcatag tcatcttcgc agtttcgcaa aaagcgtagc ccattttggt agtaaatagt
    19021 aacacgagga tcatctaggc ctgcagcaaa gtctgggaaa tactcacgac agacctcgac
    19081 caacatctca tccggttcca cgatatcgat ttgttccagt tcaggataga gtgttaatac
    19141 ttgggcaaca ccgccgtcac cacccccaat aaccaatact ttctttggat ttgggtggac
    19201 agccatggga acgtggacgg tcatttcatt gtagacgaaa tcatccgcat ctgagaacaa
    19261 gacatggcca tttaaaatca gtattttccc aaaagctggc gtatccaaga cttcgatatc
    19321 ctgccattca ctttttccag cgtaaagttg cttggctgtt ctcagagaca atttgacatc
    19381 tggagtatga acttcagaaa accataaatc catctatttc tccctatatt atatttagcg
    19441 cagttagcta ctgccaaagc ccaagtggta tacttggaat aagccactat ggattagttc
    19501 atttcctcca atcgcttgac gatttccgaa aggctctaac cacagctttt gtttcaattg
    19561 gtaatgagtt tgataacgca tagacgtttg atctcgtcta aggtaacaag gcaaaaaatt
    19621 cgtggataac caagtagact tgaaatacta tatctggagg tactcccatg ctttatgtag
    19681 gcattgatat cgctaaaaat aaacacgatg ttacagcctt gaatgttcca ggaaaaactg
    19741 ttcttaaacc actcactttt tcaaataata aagctggttt tgaactctta gatctgtcta
    19801 ttcgacagat caaccaagac tgtctcatcg ctcttgaaga tactggacat tatgccttta
    19861 atctactgaa cttcctgcat gaacaaggat ataaagttta cacctacaat cctttgctca
    19921 tcaaggaatt tgccaaatca ttatcacttc gcaagactaa aacggataag aaagacgctc
    19981 atggcatcgc tcttaaactt ctttctgacc cc
//
LOCUS       DAVHEX010000045        19875 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVHEX010000045 DAVHEX010000000
VERSION     DAVHEX010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19875
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.045"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(75..884)
                     /locus_tag="VVL45_001528"
     CDS             complement(75..884)
                     /locus_tag="VVL45_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU4187687.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNTPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKS"
     gene            complement(886..1689)
                     /locus_tag="VVL45_001529"
     CDS             complement(886..1689)
                     /locus_tag="VVL45_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU4187688.1"
                     /translation="MKQLTVEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPIDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKT
                     INLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            complement(1699..3186)
                     /gene="tacF"
                     /locus_tag="VVL45_001530"
     CDS             complement(1699..3186)
                     /gene="tacF"
                     /locus_tag="VVL45_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HEU4187689.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYSYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNHIVAPEYYLLTTML
                     SETSLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFMYPVDMVINLP
                     FLINTGLIVLLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            3522..4229
                     /locus_tag="VVL45_001531"
     CDS             3522..4229
                     /locus_tag="VVL45_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEU4187690.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIENIIEAIDAYRPL
                     TSEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITGIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            4234..5256
                     /locus_tag="VVL45_001532"
     CDS             4234..5256
                     /locus_tag="VVL45_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU4187691.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPESEIVVIGRHWEKLELFSFAKECYITDNI
                     PEELAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFKNAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            5273..6142
                     /gene="licA"
                     /locus_tag="VVL45_001533"
     CDS             5273..6142
                     /gene="licA"
                     /locus_tag="VVL45_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HEU4187692.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGADFGDYGVNRYQRA
                     VKGLASYGGSDEK"
     gene            6132..7010
                     /locus_tag="VVL45_001534"
     CDS             6132..7010
                     /locus_tag="VVL45_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU4187693.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAAAHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            7022..7711
                     /locus_tag="VVL45_001535"
     CDS             7022..7711
                     /locus_tag="VVL45_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEU4187694.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            7808..8656
                     /gene="dprA"
                     /locus_tag="VVL45_001536"
     CDS             7808..8656
                     /gene="dprA"
                     /locus_tag="VVL45_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEU4187695.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPASILDGLSDGCHHLIQEGAKLLTSGQDV
                     LAEFEF"
     gene            complement(9228..10181)
                     /locus_tag="VVL45_001537"
     CDS             complement(9228..10181)
                     /locus_tag="VVL45_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISSpn8 family transposase"
                     /protein_id="HEU4187696.1"
                     /translation="MQEHYTPKGKHLTIDNRRLIERWKNENKSNREIAGLLGKAPQTI
                     HNEVKRGTTLQQVRKGLYKKVYSADYAQTVYQFNRKRSVKKLILTKEIREKILHYHKQ
                     KFSPEMMVNKKQVKVGISTIYYWFHNGHLGLTKADMLYPRKRKGVKKQASPNFKPAGK
                     SIEERPDVINLRLENGHYEIDTVLLTKIKNYCLLVLTDRRSRHQIIRLIPNKTAESVN
                     QALTLLLGEHRILSITADNGSEFKRLSEVFPEEHIYYAHAYSSWERGSNENHNRLIRR
                     WLPKGTKKTTPKEVAFIENWINNYPKKCLDYKSPSEFLLGG"
     gene            10785..11828
                     /locus_tag="VVL45_001538"
     CDS             10785..11828
                     /locus_tag="VVL45_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HEU4187697.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGYNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEHFFQYIKDKEAKEK
                     EKANKFDTESEEQ"
     gene            11995..14082
                     /gene="topA"
                     /locus_tag="VVL45_001539"
     CDS             11995..14082
                     /gene="topA"
                     /locus_tag="VVL45_001539"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEU4187698.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNDEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQKGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            14303..14662
                     /locus_tag="VVL45_001540"
     CDS             14303..14662
                     /locus_tag="VVL45_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEU4187699.1"
                     /translation="MRLIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            14755..15387
                     /locus_tag="VVL45_001541"
     CDS             14755..15387
                     /locus_tag="VVL45_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEU4187700.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDICLTAQAGSQGVVFGALTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLENRQTFIDQVGVTQLHGTKVVF"
     gene            15431..16087
                     /locus_tag="VVL45_001542"
     CDS             15431..16087
                     /locus_tag="VVL45_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEU4187701.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVHMESIRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            16106..16738
                     /locus_tag="VVL45_001543"
     CDS             16106..16738
                     /locus_tag="VVL45_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEU4187702.1"
                     /translation="MNFQLAKYSLLKKFSENIGFTTPEECGAIFKYLIENVKTDRQII
                     YSPHCHDDLGMAVANSLAAVKNGAGRVEGTINGIRERAENAALEEIAVALNIRQDYYQ
                     VETSIVLNETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEII
                     TPELVGVKIPLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALADKK"
     gene            16766..17200
                     /locus_tag="VVL45_001544"
     CDS             16766..17200
                     /locus_tag="VVL45_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HEU4187703.1"
                     /translation="MVAGTMVENPEGFHFDDLQLQIHADNDIEALVSLANMDGEKVEF
                     NATGQGSVEAIFNAIDKFFNQSVRLVSYTINVVTDGIDAQDRVLVTVENRDTETIFNA
                     AGLDFDVLKASAIAYINANTFVQKENAGEMGRSVSYHDMPSV"
     gene            17212..18249
                     /gene="leuB"
                     /locus_tag="VVL45_001545"
     CDS             17212..18249
                     /gene="leuB"
                     /locus_tag="VVL45_001545"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEU4187704.1"
                     /translation="MAKKIVALVGDGIGPEIMEAGLEVLEALAEKTGFDYEIDRRPFG
                     GADIDAAGPPLPDETLKASREADAILLAAIGSPQYDGAAVRPEQGLMALRKELNLYAN
                     IRPVKIFDSLKYLSPLKPERISGVDFVVVRELTGEIYFGDHILEERKARDINDYSYEE
                     VERIIRKAFEIARNRRKILTSIDKQNVLATSKLWRKVAEEVAQDFPDVTLEHQLVDSA
                     AMLMITNPAKFDVIVTENLFGDILSDESSVLSGTLEVMPSASHSENGPSLYEPIHGSA
                     PDIAGQGIANPTSMILSVVMMLRDSFGRYEDAERIKRAVETSLAAGILTRDIGGQAST
                     KEMTEAIIARL"
     gene            18246..18515
                     /locus_tag="VVL45_001546"
     CDS             18246..18515
                     /locus_tag="VVL45_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEU4187705.1"
                     /translation="MKLDEKIALVLLIWNVIIFLIYGIDKFKARRRAWRIQEKILLIL
                     ALTCGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            18519..18878
                     /locus_tag="VVL45_001547"
     CDS             18519..18878
                     /locus_tag="VVL45_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEU4187706.1"
                     /translation="MAGSSREHAAWVLADYGFKVVIAGSFGDIHYNNELNNGLLPIVQ
                     PREVREKLAQLKPIDQVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNTLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            19040..19610
                     /locus_tag="VVL45_001548"
                     /pseudo
     CDS             19040..19610
                     /locus_tag="VVL45_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019771122.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
     gene            19704..19769
                     /locus_tag="VVL45_001549"
                     /pseudo
     CDS             19704..19769
                     /locus_tag="VVL45_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849983.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
BASE COUNT         6168 a         3472 c         4169 g         6066 t
ORIGIN      
        1 tatgtatttc attttctact aaaattatgt ttttgaataa cctctatctt agtagtttgt
       61 ataatccccc tcaatcagct tttacgataa gctttaatac tatgactata ccattcttgc
      121 atttcttttg atggtggtgt catataatcg ccatacatct gggttaaaaa ttggtcatat
      181 tttttgggaa caggcaacat acggccctca aactcagtta aaatcagttc tttaaaggta
      241 tcaactggga agatttcttt catcccttcc ttaccgatcc caactcctcc ttcatattga
      301 ggagtgttgg ttacagcatt tttgactagt tgatcaattt tcttgtaaaa gtagcgagga
      361 ttgacaaatc ggagagcgta ccagctacat aatctaagaa aatcttttag tttgctatca
      421 ccgtgaactg ctcgtgattt tttgatataa gctagttgac gaagagccac atacttatag
      481 ctcttgtcga caatgctcaa gtctgtaaat cgatcaattg ggaagacatc gatgaaaagg
      541 ctggtatcat gacgcttgta cttaacatgg tcttctataa cagtagaagt gtccaaaatc
      601 gatgcgaaat tatggaagta ccaagaagat gtatcgtagg aaagaacctt gtagcgaggg
      661 tgattttctt cttcaataat cttcagtaaa cgctcataat cctcacgata aagggaaata
      721 tcaatatcat catcccaagg aatcatacct ttgtggcgga tggctccaag catggttcca
      781 taactgagaa aataaggaat atcatgtttc ttacaagtct catcaatata gtccagcagg
      841 gctagttgaa tttctttaat ttcttttttt tctaaatatt gcatcctaat cctccaattt
      901 ataagcgtga aattcatgac tgtagaagcg tttttcttct ggtggtaggg tcatataatc
      961 tccataaaat tgtgtcaaaa tagtatcaaa tttttcaggt gcaggaaggc ttaaattctc
     1021 aaagggtaaa ttgattgttt tatcaaaggt accacttggg aagacttcct tttccttaaa
     1081 ttttgaaggg ataaaagcca tatactgccc attttcacga ctatattttt gaatttcttt
     1141 ctcgatttta tttgcaaaat aacgaggaga aaccggtcga aggagcaacc aaaaggctgt
     1201 tcgtatccaa tcttttaaaa ggctatcctt atagacaata tttttatgtt tactgaaaga
     1261 cagcagtttg aagctttcca gtttataaca agtatcaatg accttaggat catcaaagcg
     1321 atctataggg aaaatatcga taaagatacc agactcatag gtttttgtat ttcgagtatc
     1381 aataatttta gtcgtactgt cggttatctt gataaagttg ttaaagtagt tcttatcagt
     1441 ttctaaggat aggagcttat acttgctttt ttccttttga aaaatgttaa taaatcgttg
     1501 gtagtcttct ctaggcatgg acagatcaat atcgtcgtcc caagggataa agccctcatg
     1561 tcgaaccgcc ccaatcagag taccgtagtt aataatatag ttgatattgt gctttttaca
     1621 gagagtatca atataatcca aaatttctaa ttcaatttgt ttggcatctt caacggttag
     1681 ttgtttcatt ttaaactcct atgatttttt aaatttattt tttaaggcta ggacatggtt
     1741 taaaaattca tagaaaatgc tatcttttgt gaagacaagt agactaatat aagagatagc
     1801 tgatagcaag acaatcaaac cagtattaat caaaaatggc aaattaatga ccatatccac
     1861 aggatacatg aaattaatca ggaaataaat tgctacaaag gaaagtgaaa agagagagta
     1921 tcgaacagta tagctaaaga tatgtcccaa gtggatgagt tgttttctat ggatgaaaat
     1981 gatatagaaa acaagtagag aagtctctga taacatagtt gtcagtaagt agtattcagg
     2041 agccacgata tggttgaaaa agaggagact attcaagccc aaatttagta gcccagcaaa
     2101 gactgtatag actgtgatac gtttttcata gccattggta aagagaattt gggaaccaag
     2161 aatggtatct aaggccagaa taatcgtacg aaaagcgaag agagaggtca agatgccgcc
     2221 tccgatatat ttttcactac cgtaaagtag gatggcattt ggtcctaaaa ccatgagtcc
     2281 aaaactcagt ggaatgataa agaagttaaa gattcgacta cctctattaa ccagagaaac
     2341 ataggcttct ttgtctcctt tccccagata gtaactgaga cgaggcacac tcactccaat
     2401 tgcacctgtt acaaccccag ctataacggt cacaattcgc tgagctatgg tatagtaact
     2461 aacgttgaca tcaatccctg ttttaacgag gaagaggcga tctaaaaaag tgaagagcat
     2521 attggcattg gcaaagacta acatggctgt cagagggaga aagagtggtt taaaatcact
     2581 taggtgaatc ttaacaagtt tgatgtctct tttaatccaa aaataactaa tcaggtagtt
     2641 aatcagcgtc gataaactca tcacaagtgt atagacaaca atatcgtgtt catttttaac
     2701 aaataagaaa atagagacca gcatcaggat acggatgaag gcagttttgt aaaagagaaa
     2761 actgtaattt tccagagctt cattgaccca ttcgattgaa aaaatctggg caatgagttg
     2821 aatccccata acaaggtaga cctttttgac gattggatta tcagtaaaga agagaggata
     2881 ggctaggata tagacagcag tggtcaaaat cgtacaagcg atgcacaaat aaaaaagact
     2941 agaaaaggtt ctgttaagat cttttttgtt atccttgaca ttactgatag cccttaaacc
     3001 gtagctatag acaccataag ttgcaaaggg caagaaaaat gacaaaatag tgtcgactga
     3061 gttgaagtaa ccatagtcag ttcggtccaa gacacgcgcg acataggttc cagttaggat
     3121 gggaaaaata atattcaaga cacgaattcc catgtaagat agagcattta attttatact
     3181 tttcattcaa tttacctcgt ttttcattat atcataaagt tagctaataa gaaatgaagg
     3241 gcagtaagcc aagcaatcac tttgaagttt caaatcttaa attttaagtt ttctttaagg
     3301 aaagtatatt attctgaagg actctaaaat ttcgcagtca tttattagta attgctacag
     3361 aattcctagt cattactaga aatggactag tttctttgaa taatagaact gcataattct
     3421 cctattctag aaggggagga ccagtatttc ttttatgata ggactagatt gtggtataat
     3481 agagagaata agttttttta gtaagacaaa ggagaaaata gatgatttat gcaggaattc
     3541 ttgccggtgg aactggcaca cgcatgggga tcagtaactt gccaaaacaa tttttagagc
     3601 taggtgatcg acctattttg attcatacaa ttgaaaaatt tgtcttggag ccaagtattg
     3661 aaaaaattgt agttggtgtt catggagact gggtttctca tgcagaagat cttgtagata
     3721 aatatcttcc tctttataag gaacgtatca tcattacaaa gggtggtgct gaccgcaata
     3781 caagtattga gaacatcatt gaagccattg atgcttatcg tccgcttact tcagaggata
     3841 tcgttgttac ccacgattct gttcgtccat ttattacact tcgcatgatt caggacaata
     3901 tccaacttgc ccaaaatcat gacgcagtgg acacagtggt agaagcagtg gatactatcg
     3961 ttgaaagtac caatgggcaa ttcattacag gtattccaaa tcgtgctcac ctctatcagg
     4021 gacaaacacc tcaaacattc cgttgcaagg acttcatgga cctttatgga tctctttctg
     4081 atgaagagaa ggaaatcttg acagatgcat gtaaaatctt tgtgatcaaa ggaaaagatg
     4141 tggccttggc caaaggtgaa tactcaaatc tgaagattac aaccgtaaca gatttgaaga
     4201 ttgcaaaaag tatgattgag aaagactagt aaaatgatta atcaaattta tcaactaact
     4261 aagcctaagt ttatcaatgt caaatatcag gaagaggcta ttgaccaaga gaatcatatc
     4321 cttatccgtc ccaactacat ggctgtctgt catgcggatc agcgttacta tcagggaaaa
     4381 cgtgatccca agattttgaa taaaaagctt ccaatggcaa tgattcacga gtcatgtgga
     4441 accgtcattt ctgacccgac cggaacctac gaggttggtc aaaaagttgt catgattccc
     4501 aatcagtctc ctatgcagag tgatgaagaa ttctatgaaa actacatgac agggacccat
     4561 ttcttgtcta gtggatttga tggctttatg agagagtttg tttctctccc taaagatcgt
     4621 gtggtggctt atgatgctat tgaagatacg gttgcagcca ttacagagtt tgtcagtgtg
     4681 ggcatgcacg ctatgaatcg tctattgact cttgctcata gcaagcggga gcggatcgcc
     4741 gttattggag atggaagttt agcttttgtg gttgccaata ttatcaacta tactttgcca
     4801 gaatcagaga ttgtggttat tggtcgtcat tgggaaaagt tggaactctt ctcatttgcc
     4861 aaagaatgct atattacgga taatattcct gaagagttgg cctttgacca tgcttttgaa
     4921 tgttgtggtg gtgatggtac tggaccagct attaatgact tgattcgcta cattcgtcct
     4981 cagggaacga ttctcatgat gggagttagc gaatataaag tcaatctcaa tactcgcgat
     5041 gccttagaaa agggcttgat tttggttggg tcatctcgtt ctggtcgcat tgattttaaa
     5101 aatgctatcc aaatgatgga agtcaagaaa tttgccaatc gtcttaaaaa tatcctttat
     5161 ctagaagaac ctgtaagaga aattaaagat attcatcgtg tctttgcaac cgatttaaac
     5221 acagccttta aaacagtgtt taagtgggaa gtataagtac tggaggttaa ttgtggagaa
     5281 aatcattaaa gaaaaaattt cttccttact tagtcaagaa gaggaagtcc tcagtgttga
     5341 acaactgggt ggaatgacca atcaaaacta tttggccaaa acaacaaata agcaatacat
     5401 tgttaaattc tttggtaaag ggacagaaaa gcttatcaat cgacaagatg aaaagtacaa
     5461 tcttgaacta ctaaaggatt taggcttaga tgtaaaaaat tatctttttg atattgaagc
     5521 tggtatcaaa gtaaatgagt atatcgaatc tgcgattacg cttgattcaa cgtcaatcaa
     5581 gaccaagttc gacaaaattg ctccaatatt acaaactatt catacgtctg ctaaggaatt
     5641 aagaggagaa tttgctcctt ttgaagaaat caaaaaatac gaatccttga ttgaagaaca
     5701 aattccttat gccaactatg aatctgttag aaatgcagtc ttctccttag agaaaagact
     5761 ggctgactta ggtgttgaca gaaaatcttg tcatatcgat ttggtgcctg aaaactttat
     5821 cgaatcacct caaggacgac tttatttgat tgactgggaa tattcatcaa tgaatgatcc
     5881 aatgtgggat ttggctgccc tctttttaga gtctgaattc acttcccaag aggaagaaac
     5941 tttcttatct cactatgaga gtgaccaaac accggtttct catgaaaaga ttgctattta
     6001 taaaatttta caagatacta tttggagtct atggactgtc tataaggaag agcaaggtgc
     6061 agattttggt gactatggtg tgaatcgtta ccaaagagct gttaaaggtt tggcttctta
     6121 tggaggttca gatgaaaagt aaaaacggag ttccttttgg ccttctctca ggtattttct
     6181 ggggcttggg tctaacggtt agtgcttata tcttttcgat ttttacagat ttgtcaccct
     6241 ttgtggtggc tgcagctcat gattttttga gcatctttat cttactagct tttctcttgg
     6301 taaaagaagg gaaagttcgc ctctcaattt tcttaaatat tcgcaatgtc agtgttatca
     6361 tcggagcctt gctagcaggc cctatcggta tgcaggccaa tctttatgca gttaagtata
     6421 tcggaagttc tttagcttca tctgtatcgg ctatttaccc tgcgatttca gttctattgg
     6481 ctttcttctt tttgaagcac aagatttcga aaaatactgt atttgggatt gtcttgatta
     6541 ttggagggat tattgcccag acctataagg ttgaacaggt taattccttc tacattggga
     6601 ttctctgtgc tttggtttgt gctattgcat ggggaagtga gagtgttctt agctcctttg
     6661 ctatggaaag tgaactgagt gaaatcgaag ccctcttaat ccgtcaagtg acttcgttct
     6721 tgtcctatct tgtgattgtg ctcttctctc atcagtcatt tactgcagta gccaatggac
     6781 aattgctagg tctcatgatt gtttttgcag cctttgatat gatttcctac ttggcttatt
     6841 atatcgctat caatcgcttg caaccagcca aggctacagg cttgaacgtg agctatgtag
     6901 tatggacggt cttgtttgca gttgtttttt tgggtgcacc gctagatatg ctgaccatta
     6961 tgacgtcact tgtcgtcatt gctggagttt atattattat taaagaataa aggagattcg
     7021 tgtgaaagcc attatcttag cagcgggatt gggaactcgc ttgcgtccta tgactgaaaa
     7081 tacccctaaa gccttggttc aggttaatca aaaacctttg attgaatacc aaattgagtt
     7141 tctcaaagaa aaaggaatca atgacatcat catcattgtt ggttatctta aagaacaatt
     7201 cgattactta aaagaaaaat acggtgttcg cctcgttttc aatgataaat acgctgacta
     7261 caataacttt tactctctct atcttgtaaa agaagaattg gccaacagct atgttattga
     7321 tgctgacaat tatctcttta aaaatatgtt ccgcaatgat ttgacacgtt cgacttattt
     7381 tagtgtttat cgtgaagatt gtaccaacga atggttcttg gtctatggag atgactacaa
     7441 ggttcaagac attattgttg atagcaaggc aggtcgcatc cttagtggtg tatccttctg
     7501 ggatgctcca actgcagaaa agattgtcag ctttatcgac aaggcttatg caagtggtga
     7561 atttgttgat ctctattggg acaatatggt taaggataat atcaaagagc tagatgtcta
     7621 tgttgaagaa ttagaaggca atagcattta tgagatcgat agtgtccaag actatcgtaa
     7681 attagaagaa attcttaaaa acgaaaatta aaagcttcca acatctgaca aaatagtcgg
     7741 atgttttttg attttttacg aacttttacg aatagataga tgagtagaaa aagaaatgga
     7801 gttatttatg aaaatcacaa actatgaaat ctataagtta aaaaaatcag gtttgaccaa
     7861 tcaacagatt ttgaaagtgc tagaatacgg tgaaaatgtt gatcaggagc ttttgttggg
     7921 tgatattgca gatatatcag gttgccgtaa tccagccgtt tttatggaac gttattttca
     7981 gatagacgat gcgcatttgt cgaaagagtt tcaaaaattt ccatctttct ctattttaga
     8041 tgactgttat ccttgggatt tgagtgaaat atatgatgcg cctgtacttt tattttacaa
     8101 gggaaatctt gacctcctga aattcccgaa ggtagcggtc gtgggcagtc gtgcttgtag
     8161 caaacaggga gctaagtcag ttgaaaaagt cattcaaggc ttggaaaatg aactggttat
     8221 tgtcagtggt ctggccaagg gcattgacac agcagctcat atggcagctc ttcagaatgg
     8281 cggaaaaacc attgcagtga ttggaacagg actggatgtg ttttatccta aagccaataa
     8341 acgcttgcaa gactacatcg gcaatgacca tctggttcta agtgaatatg gacctggcga
     8401 acaacctctg aaatttcatt ttcctgcccg taatcgcatc attgctggac tttgtcgtgg
     8461 tgtgattgta gcagaggcta agatgcgttc aggtagtctc attacgtgtg agcgagcaat
     8521 ggaagaagga cgcgatgtct ttgctattcc tgctagcatt ttagatggac tatcagacgg
     8581 ttgccatcat ttgattcaag aaggagcaaa attgctcacc agtgggcaag atgttcttgc
     8641 ggaatttgaa ttttaaaaat gacctaagct agaattctaa gaaaaaatca attttaagag
     8701 aaaatgaacc caacatttcc ataataaaac gcatattagc aagtttttaa cacttgctaa
     8761 tatgcgtttt ttctaagtgg attagtagag tagaggattt ttctcatata atactcttcg
     8821 aaaatctctt caaactacgt cagcttccat ctgcaacctc aaaacagtat tttgagctga
     8881 gttcgtcagt cttatctaca acctcaaagc agtgctttga gcaacctgtg gctagcttct
     8941 tagtttgcgc tttgattttc gttgagtata agggaaagta tagtgaattg aaataagatg
     9001 tgaacaactc tatcaggaaa gtcaaattaa tttatagaaa tattttagca gccaaggtgc
     9061 actgttatag attcaataca ctataattta gtgtaattga gaaaggagaa atgattgtga
     9121 ttgatgttgg ctaggttatg ttcaatgatt cctaccatct caaatcttgt tagtaaggaa
     9181 aaataaattc ttcaaaagta gagattaccc caaatttcaa gttgaagtta gccacccaaa
     9241 agaaattcac ttggcgactt gtagtccaag cattttttag ggtagttgtt aatccaattt
     9301 tcgataaaag ctacttcttt cggagtcgtt ttcttggttc ctttaggtaa ccatctccga
     9361 attaatcgat tatgattttc atttgaacct ctctcccatg aagagtaagc atgtgcgtag
     9421 tagatatgtt cctcaggaaa tacctcagac aatcgtttga actccgaacc attatctgca
     9481 gtaatggaca gaatacgatg ctcccctaat agtaacgtaa gcgcctgatt gacagattca
     9541 gcagttttat ttggaattaa ccttataatt tggtgtctgc tccgccggtc ggttaagact
     9601 aacaggcaat aattttttat cttagtcagt aggacggtat caatttcata atgaccattt
     9661 tccaagcgaa gattaataac gtcaggacgt tcttcgatag atttacctgc cggcttaaag
     9721 ttcggactag cttgcttctt gacacctttc ctttttctgg gataaagcat gtcggccttc
     9781 gtcaatccta aatgaccatt atgaaaccag tagtagatgg ttgaaatacc aactttcact
     9841 tgcttcttgt taaccatcat ttcaggcgaa aatttttgct tatgatagtg taagatcttc
     9901 tctctgattt cctttgttaa aattaacttt ttcaccgacc gttttcgatt gaattggtaa
     9961 acagtttgtg cgtaatcggc agaatagacc tttttgtata gcccttttct cacttgttgt
    10021 aaagttgtac ctcttttgac ttcattatga atcgtttgag gcgcctttcc taacaagcct
    10081 gcaatttcac gattggactt attttcattc ttccaccgct caatcaagcg acggttatct
    10141 attgtcaaat gtttcccttt tggggtataa tgttcttgca tctctgtgcc tttccttgtg
    10201 tttgtggtga acaacaagta tagcacagag gtgttttctt ataccttaaa tcacatttca
    10261 tttgacaaca ggaacccttg aacctgtttt ttcagctaaa ccaaggctat gattagcctt
    10321 ggccacctag cctaacagtt tcaaacctgt tatcaaatga aatcatcaac ttactcaaac
    10381 atagtgaaac atctaattct tgactaaggt aaggtggcta acttcattat agaactttca
    10441 aagtagagat tacaaggctt gtttaagaaa gaattcaaag accttgacaa ataaaaataa
    10501 aatggttatt ataaaaaatg gtctgaaata gatgatgata cttttcgaaa atctttttac
    10561 gtcagctcag ctttgccttg ctgtgttttg agcaagctac ggttagcttc cgagtttgat
    10621 tttcatttac tagaaatgaa actgatgaga gatatcagta gacatttgag tcaggatatt
    10681 atggaaaatg ataaaaagag ctcgtgagat tggcatatca gactactaaa gtattgagtt
    10741 tgttaggatt ttagcgacta gttagctggg aaaggaagat atttgtgaca aataataaac
    10801 tgtattcgtt gatagaattt agaaataaaa tatatgaaga attagaactt tccagaagtg
    10861 atttagcgat tttactatgt gccatgctta tcgcctctat cggattaaat atggattcga
    10921 ctcccgtgat tattggagcc atgttaatct ctcctttgat gacacctatt ctgggagtgg
    10981 ggctctctct agctatattt gattttaaat tgttaagaaa atcttttaaa atattagcta
    11041 ttcaaattct tgccagttta atagcttcaa cactttattt ttatctttct cccatttcgt
    11101 atgctagttc ggaaattgtt gctagaacct ctccgactat ttgggatgtt ctcattgctt
    11161 ttgtaggagg gatagcaggt attattggtg ctaggaaaaa agagaccaat aatattgttc
    11221 ctggtgttgc tattgcaacc gccttgatgc ctcctctttg tacagtaggt tatgctattg
    11281 cttctgctaa tctaaaattt atcataggct cctcttacct attcctcatc aattgtagct
    11341 ttattgtcat tgcgacttat ataggtgtta ggttgatgat ggttaagaaa cattatttta
    11401 aagataatga agaagactct aaaatgcgta ggattttgct tctagttgct gttttgctga
    11461 tgattccgag tttcatctct gcaacgactt tagtgagaga aacgttgaaa aaagagtccc
    11521 ttaagaaatt tatatcagag cagtttcagg ggtataatat tttgaaaaaa acctattcta
    11581 aaaagactca taccctaaag ctaaccattt caggaaatta tttgacagaa gaagaactcg
    11641 atatgatttc aagtaagaga ggtgactatg gtttaagtga tgtttctgtt caagtttcac
    11701 aattgtctga ttcagaacaa cttagtaagg aagagctggt ggagcatttc ttccagtata
    11761 tcaaggataa ggaagcaaaa gaaaaggaga aagccaataa atttgataca gagtctgagg
    11821 agcaataatt tcttgagaat agctggtttc tctcgtgagt cttctatgta tatcaaagga
    11881 agactgaggt tttaagtata aaacttttct tctattatag tagctaagtc ttgacagggt
    11941 tcaaaaaatg gtttacactt tataaagttt attactttga aaaggtgtga tactgtggct
    12001 acggcaacaa aaaagaaaaa atcaacagtt aaaaaaaatc tagtcatcgt ggagtcgcct
    12061 gctaaggcca agacgattga aaaatatcta ggcagaaact acaaggtttt agccagtgtc
    12121 gggcatatcc gtgatttgaa gaaatccagt atgtccgtcg atattgaaaa taattatgaa
    12181 ccgcaatata ttaatatccg aggaaaaggc cctcttatca atgacttgaa aaaagaagct
    12241 aaaaaagcta ataaagtttt tctcgcgagt gacccggacc gtgaaggaga agcgatttct
    12301 tggcatttgg cccatattct caacttggat gaaaatgatg ccaaccgtgt ggtcttcaat
    12361 gaaatcacca aggatgcagt caaaaatgct tttaaagaac ctcgtaagat cgatatggac
    12421 ttggtcgatg cccaacaagc tcgtcggatc ttggatcgct tggtagggta ttcgatttcg
    12481 cctattttgt ggaagaaggt caagaagggc ttgtcagcag gtcgcgttca gtccattgcc
    12541 cttaaactca tcattgaccg tgaaaatgaa atcaatgcct tccagccaga agaatactgg
    12601 acagttgatg ctgtctttaa aaagggaacc aaacaatttc atgcttcctt ctatggagta
    12661 gatggtaaaa agatgaaact gaccagcaat gacgaagtca aggaagtctt gtctcgtctg
    12721 actagtaaag acttttcagt agatcaggtg gataagaaag agcgcaagcg caatgctcct
    12781 ttaccctata ccacttcatc tatgcagatg gatgctgcca ataaaatcaa tttccgtact
    12841 cgaaaaacca tgatggttgc ccaacagctc tatgaaggaa ttaatatcgg ttctggtgtt
    12901 caaggtttga ttacctatat gcgtaccgat tcgactcgta tcagtcctgt agcgcaaaat
    12961 gaggcggcaa gcttcattac ggatcgtttt ggtagtaagt attctaagca cggtagcaag
    13021 gtcaaaaacg catcaggtgc tcaggatgcc catgaggcta ttcgtccgtc aagtgtcttt
    13081 aatacaccag aaagcatcgc taagtatctg gacaaggatc agctcaagct atataccctt
    13141 atctggaatc gttttgtggc tagccagatg acagcggccg tttttgatac catggctgtt
    13201 aaattgtctc aaaaaggggt tcaatttgct gccaatggta gtcaggttaa gtttgatggt
    13261 tatcttgcca tttataatga ttctgacaag aataagatgt taccggacat ggttgttgga
    13321 gatgtggtca aacaggtcaa tagcaaacca gagcaacatt tcacccaacc gcctgcccgt
    13381 tattctgaag caacactgat taaaacctta gaggaaaatg gggttggacg tccatcaacc
    13441 tacgcgccaa ccattgaaac cattcagaaa cgttattatg ttcgcctggc agccaaacgt
    13501 tttgaaccga cagagttggg agaaattgtc aataagctca tcgttgaata tttcccagat
    13561 atcgtaaacg tgaccttcac agctgaaatg gaaggtaaac tggatgatgt cgaagttgga
    13621 aaagagcagt ggcgacgggt cattgatgcc ttttacaaac cattctctaa agaagttgcc
    13681 aaggctgaag aagaaatgga aaaaatccag attaaggatg aaccagctgg atttgactgt
    13741 gaagtgtgtg gcagtccaat ggtcattaaa cttggtcgtt ttggtaaatt ctacgcttgt
    13801 agcaatttcc cagattgccg tcatacccaa gcaatcgtga aagagattgg tgttgagtgt
    13861 ccaagctgtc atcagggaca aattattgag cgaaaaacca agcgtaatcg cctattctat
    13921 ggttgcaatc gctatccaga atgtgaattt acctcttggg acaagcctgt tggtcgtgac
    13981 tgtccaaaat gtggcaactt cctcatggag aaaaaagtcc gtggtggtgg caagcaggtt
    14041 gtttgtagca aaggcgacta cgaggaagaa aagattaaat aagaggaggg gcccgaaagt
    14101 aaatttcagg ctccttttgg ttagaacttg acaaaataca tcattttatg cgaaactaga
    14161 agaagattat tttatactca atgagaatca aagagcaaat tagaaagcta gtcgcaggtt
    14221 gctcaaagca ctactttgag gttgaagatg gaagctgacc tggtttgaag agattttcga
    14281 agagtattaa ctaggagaag ttatgcgtct tatctatcta attattggtt ttttatcgct
    14341 ggccttggct attgttgggg ttgttttacc cttgttgccg acaacgcctt tccttttgtt
    14401 gtctattgct tgtttctcca gaagttccaa gcgtttcgaa gactggcttt atcataccaa
    14461 gctctatcaa acatatgtag ctgattttcg cgagactaag tctattacgc gtgaacgaaa
    14521 gaaaaaaatc atcgtctcta tctacgtctt gatgggaatt tctatttatt ttgcacctct
    14581 tttaccagtc aaaatcggtc tgggtgcttt gaccatcttt atcacttatt atctcttcaa
    14641 ggtcattcca gacaaagaat agttaaaaca gtagttattt gccttgataa aattgaaagc
    14701 atattcataa caatatgata taataaattt gaagtaatat tcaaggagaa tcaaatgatt
    14761 tacgaatttt gtgctgaaaa tgtgacttta cttgaaaaag cgatgcaggc tggagctcgt
    14821 cggattgaac tctgtgataa tctagcagtt ggtgggacaa cacccagcta tggagtgact
    14881 aaggcagcgg ttgaactggc agctaactac gatacaacca tcatgaccat gattcggcca
    14941 cgtggtggtg actttgtcta taatgaccta gaaattgcta tcatgctaga agacatttgt
    15001 ttgactgctc aggctggaag tcaaggggtt gtatttggag ctttaactgc tgataaaaag
    15061 ttggataagc ctaatctgga aaagttaatt gctgcatcaa aaggaatgga aattgtcttt
    15121 cacatggcct ttgatgaact aagtgatgaa gatcaaccgg aagctattga ctggctcagt
    15181 caagccggtg tcactcgtat cctaactcgt gctggtgtgt ctggcgactc cttagaaaaa
    15241 cgttttgttc actatcacag aattttggag tacgctaaag gtaaaattga aattctacca
    15301 ggtgggggga ttgaccttga aaaccgtcaa acctttatcg accaggtggg ggtaacacaa
    15361 ttgcatggta ctaaggttgt tttttaaaaa atagaaagga actgctagcc ttgggtagca
    15421 gttttcactt atgtttgaaa tttttaaatc ctatcaattt aatcaagaaa aggctcatga
    15481 ttatggtttt atagaaaata gcgaagtctg gacatatagt tgccagattt tgcaaggtga
    15541 ctttgtcatg actgtgtcca tcactgctga taatgtgaac tttcaagtct ttgaccaaga
    15601 gactggtgac ctctatcctc acgttcatat ggaaagcata aggggaagtt ttgtcggaaa
    15661 tgtccgtgag gcttgtctgg agattcttta ccagattcgg aaggcttgtt ttgatgtgca
    15721 agattttatc tgtcatcaga ctaagcgtat catgactcaa gttcaggaaa agtatggaaa
    15781 ccagttggag tatctgtggg aaaaatcgcc tgatacagct gtattgcgcc atgaaggcaa
    15841 tcaaaagtgg tatgccgtct tgatgaaaat ctcttggaat aagctggaaa agggcagaga
    15901 aggacaagtg gaagcagtca acctcaagca tgaccaagta gctaatttgc tttcacaaaa
    15961 ggggatttat ccagccttcc atatgagcaa gcgctactgg attagtgtgt cccttgatga
    16021 tactttatca gatgaagaag tactggaatt gatagaaaaa agttggaact taacctctaa
    16081 aaaatgaaat attttaataa ttttcatgaa ctttcaatta gctaaatatt ctttactgaa
    16141 gaaattttca gaaaatatag gatttaccac accagaggaa tgtggtgcca tcttcaaata
    16201 cctgattgag aatgtcaaga cggatcgtca gatcatctat tcgcctcact gtcatgatga
    16261 cctcggaatg gcagtggcaa atagccttgc tgctgtcaag aatggtgcag gacgtgttga
    16321 agggactatc aatggtatta gggagcgagc tgaaaatgct gctttggaag aaattgcagt
    16381 ggctctcaat attcgccaag attactacca agtagaaacc agtattgtcc taaatgagac
    16441 catcaatacg tcagaaatgg tttctcgctt ctctggtatt ccagttccta aaaacaaagc
    16501 cgtcgttggt ggcaatacct tctcccacga atctggtatt caccaagatg gagtccttaa
    16561 aaatcctctc acttatgaga tcatcacacc tgaattggtt ggtgttaaga ttccgcttgg
    16621 aaaattatct ggtcgccatg cttttgttga gaaactgaga gaattggccc tagattttac
    16681 agaagaggat atcaaaccac tctttgctaa gttcaaggca ctggccgata agaagtaaga
    16741 aatcacagat gcagatactc gagctttggt agctggaacc atggttgaaa atccagaagg
    16801 cttccacttt gatgatttac aacttcaaat tcatgcagat aatgacattg aagcgctcgt
    16861 tagcctagcc aatatggatg gtgagaaagt cgaatttaat gcgacagggc aaggttccgt
    16921 tgaagcaatc tttaacgcta tcgataagtt ctttaaccaa tctgttcgtt tggtgtccta
    16981 cactatcaat gtggtaacag atggaatcga tgcccaggat cgggttttgg tcactgttga
    17041 aaacagagat acagaaacca tctttaatgc agcagggctt gattttgatg tgttgaaggc
    17101 ttctgctatt gcctatataa acgctaatac ctttgttcaa aaagagaatg caggtgagat
    17161 gggacgcagt gtttcttacc acgatatgcc tagtgtgtaa aggagaaggc tatggcaaag
    17221 aaaatagtag ctctagtagg agacggaatt ggcccagaaa tcatggaggc tggtttagaa
    17281 gttctggagg ctctagctga aaaaacaggt tttgactatg agattgacag acgaccgttc
    17341 ggaggtgcag atattgatgc agcaggacct cccttacctg atgaaaccct taaggcaagt
    17401 agggaagcag atgctatcct actagcagct atcggtagtc ctcagtatga tggagcagcg
    17461 gttcgccctg aacaaggcct gatggctctc cgtaaggaac tcaatcttta cgctaatatt
    17521 cgtcctgtaa aaatctttga cagtctcaag tatttgtcac cactcaaacc ggaacgaatt
    17581 tctggtgtag actttgtcgt ggtgcgtgaa ttgacaggcg agatttactt tggagatcat
    17641 attcttgaag agcgcaaagc gcgtgatatc aacgactata gctatgagga agtggagcgg
    17701 attattcgca aagcctttga aattgcaaga aatcgcagaa aaatccttac tagtatcgat
    17761 aagcaaaatg ttctagcgac ctcaaaactc tggcggaaag tagctgagga agtcgcacag
    17821 gatttcccag atgtaacctt ggaacaccag ctggtagact cagctgctat gcttatgatt
    17881 accaatcctg ctaagtttga tgttattgta acggagaatc tttttggaga tattttatct
    17941 gatgaatcaa gcgtcttatc tggtacactt gaggttatgc catcagccag tcattctgaa
    18001 aatggaccaa gtctctatga acctattcac ggttcagcac ctgatattgc aggtcaagga
    18061 attgccaatc ctacttccat gattttatca gttgtcatga tgttgagaga tagtttcgga
    18121 cgttatgagg atgcagagcg tatcaaacgt gctgttgaga caagtctggc ggcaggaatt
    18181 ttaacgagag atataggagg tcaggcttca acaaaggaaa tgacggaagc tattattgca
    18241 aggttatgaa gttagacgaa aaaattgctc tagtcctttt gatttggaat gtcatcattt
    18301 tcttgattta tggcattgac aaattcaagg caaggagaag agcttggcgc atccaagaga
    18361 aaatcttact cattttagcc cttacttgtg gtggttttgg ggcctggtta gctggaatca
    18421 cttttcacca caagactcga aaatggtact ttaaaacagt ttggtttctt gggatggtga
    18481 ccacactagt agccttatat tttatttgga ggtaatggat ggcagggtct tcgagggaac
    18541 acgctgcttg ggttctagcg gactatggtt ttaaggtcgt gattgcagga tctttcggtg
    18601 acattcatta caataatgaa ctcaataatg gcctgttgcc aatcgttcag cctagagagg
    18661 ttagagagaa attagcccag ctaaaaccaa tcgaccaggt aactgtggac ttggaacaac
    18721 aaaaaatcat ctcaccagtt gaagaattca ccttcgagat agatagcgag tggaaacata
    18781 aactcctaaa tactttggat gatatcggta ttaccttgca gtatgaagag ttgattgctg
    18841 cttatgaaaa acaacgacca gcctactggc aggattagaa aaaatagaaa aggaaatata
    18901 gtaaactgaa ataagatgta aacaaatgaa ttagagctta acatccattt ccagcaattt
    18961 tttagaaact acagtggact attctggatt caacacatta taaaattatg acaaaacaca
    19021 ttcacaagaa ggctacgaca ttttaaaagg tgagggcgga tgtatcgttt gccctactaa
    19081 agttggttac attatcatga ccagtgacaa ggcaggactt gagcgtaagt tcgcagccaa
    19141 agaacgtaag cgtaacaaac caggtgttgt tctctgcggt agcatggatg aacttcgcgc
    19201 tttagcgcaa ctcaacccag aaattgaagc attctactaa aaacattggg atgaagatat
    19261 tcttcttggt tgtatccttc cttggaaacc agaagcctct gaaaaactca aagcatacgg
    19321 ggatggccgt gaagaactta tgactgatgt acgtggtact agctgttttg ttatcaagtt
    19381 tggaaaagca ggtgaacaat tggctgccaa gctttgggaa gaaggtaaaa tggtctacgc
    19441 ctcatctgct tcaatgacaa aacgattgaa actcgctatg agagcaaggt gtaatggtgt
    19501 ctatggtcga taaggacggc aaactcatcc cagaacaagg aggagcacgt tcaacttcac
    19561 cagcaccagt tgtgatccgt aaagggcttg acattgatat agtaaaatga agtaagagca
    19621 ggacaaatta atcagaacag tcaaatcgat ttctaacaat gttttagaag tagaggtgta
    19681 ctattctagt ttcaatccac tataaaatca tgatgcacct gtcagatact tttaactcat
    19741 gggactaccg tcaggttgag tattattagg atagagaaga agtctagtgt taaggacatt
    19801 acagctcata atactaggct tttttataga aattcttttc tttttctata ggatatggta
    19861 ttctatgtag aaaat
//
LOCUS       DAVHEX010000046        19602 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVHEX010000046 DAVHEX010000000
VERSION     DAVHEX010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19602
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.046"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            330..1847
                     /gene="malQ"
                     /locus_tag="VVL45_001550"
     CDS             330..1847
                     /gene="malQ"
                     /locus_tag="VVL45_001550"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEU4187707.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLEASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEYFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            1873..4131
                     /gene="glgP"
                     /locus_tag="VVL45_001551"
     CDS             1873..4131
                     /gene="glgP"
                     /locus_tag="VVL45_001551"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEU4187708.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIDLDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAVVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERFYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(5175..5747)
                     /locus_tag="VVL45_001552"
     CDS             complement(5175..5747)
                     /locus_tag="VVL45_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187709.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAVWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     FAMNLLSPGTYLAQLWDKNDAIPNNGRINF"
     gene            complement(6636..7484)
                     /locus_tag="VVL45_001553"
     CDS             complement(6636..7484)
                     /locus_tag="VVL45_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU4187710.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLVPQIKQSANYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSKLTEITIEAEI
                     LVGKAF"
     gene            7636..7914
                     /locus_tag="VVL45_001554"
     CDS             7636..7914
                     /locus_tag="VVL45_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187711.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            7916..9979
                     /locus_tag="VVL45_001555"
     CDS             7916..9979
                     /locus_tag="VVL45_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU4187712.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWFSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(10057..11313)
                     /locus_tag="VVL45_001556"
     CDS             complement(10057..11313)
                     /locus_tag="VVL45_001556"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEU4187713.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADENNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQELDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            11455..13920
                     /locus_tag="VVL45_001557"
     CDS             11455..13920
                     /locus_tag="VVL45_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEU4187714.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     VIFPAILLSIKALFNLLFVLGFLGGMLGAGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPEKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            13985..14896
                     /locus_tag="VVL45_001558"
     CDS             13985..14896
                     /locus_tag="VVL45_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU4187715.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVAVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            15126..15824
                     /gene="dapD"
                     /locus_tag="VVL45_001559"
     CDS             15126..15824
                     /gene="dapD"
                     /locus_tag="VVL45_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEU4187716.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGSVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            15892..17022
                     /locus_tag="VVL45_001560"
     CDS             15892..17022
                     /locus_tag="VVL45_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HEU4187717.1"
                     /translation="MLDLIQTRRDLHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDGAFGDWLPDQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            17034..17573
                     /locus_tag="VVL45_001561"
     CDS             17034..17573
                     /locus_tag="VVL45_001561"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEU4187718.1"
                     /translation="MKSELRKQVLHEMKAISQEQKQAIDQALTERLLQHPFYQEAKVI
                     ATYLSFSHEFQTQELIEQALKDGKKVLIPKTYPKGRMDFVVYDPQQLVKTFFALLELQ
                     GDLEVVDASQIDLIHVPGLAFTTKGYRIGYGGGYYDRYLEHFSGRTLSTIYPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            17557..18234
                     /locus_tag="VVL45_001562"
     CDS             17557..18234
                     /locus_tag="VVL45_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU4187719.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGL"
     gene            18248..18325
                     /locus_tag="VVL45_001563"
                     /pseudo
     CDS             18248..18325
                     /locus_tag="VVL45_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(18535..19476)
                     /locus_tag="VVL45_001564"
     CDS             complement(18535..19476)
                     /locus_tag="VVL45_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187720.1"
                     /translation="MENKFVEFIENNKKVIISSVAVGVVLVLGFGWYSYNQQQAEQQA
                     KIVQLEKDSKSDKEQVDKLFESFDASSDESISKLKELSETSLKTDAGKDYLNNKVKES
                     SKAIVDFHLQKGLAYDVKDSDDKFKDKATLETNVKEITKQIDFIKKVDETFKQENLEE
                     TLKSLNDLVDKYQKQIELLKKEEEKAAEKAAEKAKESSSQSNSSGSASNESYNGSSNS
                     NVDYSSSEQTNGYSNNYGGQDYSGSGDSSTNGGSSEQYSSSNSNSGANNVYRYKGTGA
                     DGYQRYYYKDHNNGDVYDDDGNYLGNFGGGIAEPSQR"
BASE COUNT         5674 a         3603 c         4194 g         6131 t
ORIGIN      
        1 tctgtctaaa gagggaaatc aggaggggct accttcctat ttctaatcta tatctatttt
       61 ctatagacgt ttgtgctttg ttgcatataa attcaagctc cctaaggagg ttttgtactt
      121 tgtatactgt ctcgtgagag tcaaacttta ggattagata gttttaagtt atctttgcta
      181 aaatgagtta gtctccttaa aaataatcaa aaaagttttt taaaaacgct tgcaattatg
      241 cttgaaaagg agtatactta taagtaacgc aaacgtttgc gtctgaaaaa tacgcaacgt
      301 tccattattt taacacacga ggtgctatta tgaaaaaacg tcaaagtggt gtgttgatgc
      361 acatctcttc tcttccagga gcttacggaa tcggatcatt tggtcaaagt gcttacgact
      421 tcgttgattt cttggtccgt acaaaacaac gttactggca aatccttcca ttaggagcaa
      481 ctagttacgg ggattctcct taccaatctt tctcagcctt cgcaggaaac actcatttta
      541 tcgatttaga tatcttggtg gagcaaggtt tgttggaagc aagtgacctt gaaggagttg
      601 actttggtag cgatgcgtct gaagttgact atgctaaaat ctactatgca cgtcgtcctc
      661 ttttagaaaa agcggtgaaa cgtttctttg aagtcggaga tgttaaagat tttgagaaat
      721 ttgctcaaga caaccaatca tggcttgagc tctttgctga gtatatggct atcaaagagt
      781 attttgacaa tcttgcttgg actgaatggc cagatgcaga tgctcgtgct cgtaaagctt
      841 cagcacttga aagctatcgt gagcaattgg cagacaagtt ggtttaccac cgtgtgactc
      901 aatacttctt cttccaacaa tggttgaaat tgaaagctta cgctaacgac aaccacatcg
      961 aaatcgttgg ggacatgcca atctacgtag cggaagattc aagtgatatg tgggcaaatc
     1021 cacatctctt caaaacagat gtcaatggta aggctacttg tatcgcagga tgcccaccag
     1081 atgagttttc tgtaactggt cagctttggg gtaatccaat ctatgactgg gaagcaatgg
     1141 acaaagacgg ctacaaatgg tggattgaac gcttgcgtga aagcttcaaa atctacgata
     1201 tcgttcgtat cgaccacttc cgtggcttcg aatcttactg ggaaatccct gctggttccg
     1261 atacagcagc acctggtgag tgggtgaaag gtccaggtta caagcttttt gcagccgtta
     1321 aggaagaact tggtgagcta aacatcatcg cagaagacct tggcttcatg acagatgaag
     1381 tgatcgaatt gcgtgaacgt actggcttcc caggaatgaa gattcttcaa tttgccttca
     1441 acccagaaga cgaaagcatt gatagcccac acttggcacc tgctaactca gttatgtaca
     1501 caggaacaca cgataacaat acggttcttg gttggtaccg taatgagatt gatgatgcga
     1561 ctcgtgagta catggctcgt tacacgaacc gtaaagaata cgaaacagtg gtacacgcta
     1621 tgcttcgtac agtattttca tcagttagct ttatggcaat tgcaactatg caagatttac
     1681 tagaattgga tgaggcagct cgtatgaact tcccatctac ccttggtgga aactggtctt
     1741 ggcgtatgac tgaagatcaa ttgacaccag ctgtcgagga aggtttgctt gacttgacaa
     1801 caatttatcg ccgaattaat gaaaatttgg tagatttaaa gaaataagac aataatcagg
     1861 agacaactaa acatgttatc actacaagaa tttgtacaaa atcgttacaa taaaaccatt
     1921 gcagaatgta gcaatgaaga gctttacctt gctcttctta actacagcaa gcttgcaagc
     1981 agccaaaaac cagtcaacac tggtaagaaa aaagtttact acatctcagc tgagttcttg
     2041 attggtaaac tcttgtcaaa caacttgatt aaccttggtc tttacgacga tgttaaaaaa
     2101 gaacttgcag ctgcaggtaa agacttgatc gaagttgaag aagttgaatt ggaaccatct
     2161 cttggtaatg gtggtttggg acgtttggct gcctgcttta tcgactcaat tgctactctt
     2221 ggtttgaatg gtgacggtgt tggtcttaac taccactttg gtcttttcca acaagttctt
     2281 aaaaacaacc aacaagaaac aattccaaat gcatggttga cagagcaaaa ctggttggtt
     2341 cgctcaagcc gtagctacca agtaccattt gcagacttta ctttgacatc aactctttac
     2401 gatattgatg ttactggtta tgaaacagcg actaaaaacc gcttgcgttt gtttgacttg
     2461 gattcagttg attcttctat tattaaagat ggtatcaact ttgacaagac agatatcgct
     2521 cgcaacttaa ctctcttcct ttacccagat gatagtgacc gtcaaggtga attgctccgt
     2581 atcttccaac aatacttcat ggtttcaaac ggtgcgcaat tgatcatcga cgaagcaatc
     2641 gaaaaaggaa gcaacttgca tgaccttgct gactacgcag ttgtccaaat caacgatact
     2701 cacccatcaa tggtgattcc tgaattgatt cgtcttttga ctgcacgtgg tatcgatctt
     2761 gacgaagcaa tctcaattgt tcgtagcatg actgcctaca ctaaccacac aatccttgct
     2821 gaagcgcttg aaaaatggcc tcttgaattc ttgcaagaag tggttcctca cttggtacca
     2881 atcatcgaag aattggaccg tcgtgtgaag gcagagtaca aagatccagc tgttcaaatc
     2941 atcgatgaga gcggacgtgt tcacatggct cacatggata tccactacgg atacagtgtt
     3001 aacggggttg cagcactcca tactgaaatc ttgaaaaatt ctgagttgaa agccttctac
     3061 gacctttacc cagaaaagtt caacaacaaa acaaacggta tcactttccg tcgttggctt
     3121 atgcatgcta acccaagatt gtctcactac ttggatgaga ttcttggaga tggttggcac
     3181 catgaagcag atgagcttga aaaacttttg tcttatgaag acaaagcagt tgtcaaagaa
     3241 aaattggaaa gcatcaaggc tcacaacaaa cgtaaattgg ctcgtcactt gaaagaacac
     3301 caaggtgtgg aaatcaatcc aaattctatc tttgatatcc aaatcaaacg tcttcacgag
     3361 tacaaacgcc aacaaatgaa cgctttgtac gtgatccaca aataccttga catcaaagct
     3421 ggtaacatcc ctgctcgtcc aatcacaatc ttctttggtg gtaaagcagc tccagcctac
     3481 acaatcgctc aagacattat ccatttaatc ctttgcatgt cagaagttat tgctaacgat
     3541 ccagcagtag ctccacactt gcaagtagtt atggttgaaa actacaacgt tactgcagca
     3601 agtttcctta tcccagcatg tgatatctca gaacaaatct cacttgcttc taaagaagct
     3661 tcaggtactg gtaacatgaa attcatgttg aacggagctt tgacacttgg tactatggac
     3721 ggtgctaacg tggaaatcgc tgagttggtt ggagaagaaa acatctacat cttcggtgaa
     3781 gattcagaaa ctgttatcga cctttacgca aaagcagctt acaaatcaag cgaattctac
     3841 gctcgtgaag ctatcaaacc attggttgac ttcatcgtta gtgatgcagt tcttgcagct
     3901 ggaaacaaag agcgcttgga acgtttttac aatgaattga tcaacaaaga ctggttcatg
     3961 actcttcttg atttggaaga ctacatcaaa gtcaaagagc aaatgcttgc tgactacgaa
     4021 gaccgtgacg catggttgga taaagtcatc gttaacattt ctaaagcagg attcttctca
     4081 tctgaccgta caatcgctca gtataacgaa gacatctggc acttgaacta atactcttcg
     4141 aaaatctctt caaaccacgt cagctttatc tgcaacctca aagcagtgct ttgagcaacc
     4201 tgcggctagc ttcctagttt gctctttgat tttcattgag tataagatac aaatttatac
     4261 taatacattt tgtaaaaaag cgagtttcga ttgaaattcg cttttttaat gatgtagatt
     4321 tgggtcaatc ttgtctaaaa atagggaaat cctagataca gtgaaggctt taaatgctgg
     4381 tttttactgt cctcagcctt atattttttc gtagttggtt acctcatatc tattatattc
     4441 gcttacataa agtattataa tataattgta ggaaagaagg tgtttttatg atatacacac
     4501 ttaaattggt gttgtttatt acctttcttg taataagctt gttacctgat aagatttttg
     4561 gaaaaaataa aaaaatttgg aaaatagttt ttgcaatatt gacggcagtg gcagcattgt
     4621 catttatgta ctaagttatt ttaagaatgt agggaaataa accctacatt ctttttagtt
     4681 ttttctgttt tctaaattct atttatccaa gcgattcaac atttcttgct tcttcgcttc
     4741 aagttctgca cgcttttctt cgatttcggc atgttttttc tcgagttcag aacaacttgc
     4801 accattgcta aattcttttc gccatcagga gatagggtga gtcgacatgt ctattactca
     4861 cccaaagcag tcctacaaag caggaatttt ctgttacttt tttggaaata gtaacgttta
     4921 tacagctttg acacttcgta tcaaagcgcc aaacacactc cgagggattt acagaaagca
     4981 gaaaaggaat gatctggtat aagatcattc cttttctctc tttttcttta agtaattata
     5041 tacaatgtac gacgaagtcg tcattgcaat gctgatccac cacctaaagg gaactttaaa
     5101 caacattgat aagataaaga atataaacaa cgaaaatacg ttatacccaa ttaattttat
     5161 tgtatatctc atgattaaaa gttaatcctt ccgttgttag gaatggcatc atttttatcc
     5221 cataattgtg ctaaataagt ccccggtgat aataaattca tagcgaattc taaagcaaca
     5281 tcatttacaa accaactacc tagatatcta gaaattgctg aacgaatagc actttttgct
     5341 gcatgttttc cttttacttt aattagattt gcaaggcctg cagtagttcc tcctaatgct
     5401 aaagctattg cagtatctaa tagagcaccc atttgattaa ctgtaatacc ttgccaaact
     5461 gctctaaatg gagagtatgt aggtgggatt gtataatcgc cttgtaattg tcggttaatt
     5521 acttctttga tccattgttg tgagacgtct ggatgaaaag attggatttc gtttgcaagt
     5581 gtattgattt gttcttctgt tagagaagtg acaggttgaa gttccatatt tgtttcaatt
     5641 tgtgatactt gttcagaagc gtatacagct gaaacacttg gaatcgctga tacaattaac
     5701 acaattgacg tcaaaaaaac cgaaataaat ttcattaatt tgttcatgag cttttctcct
     5761 ttttatttgc atctgcttac attttatcat atactgttat tatagtcaaa aaaatatgct
     5821 attatgttaa aaaaatattt ttcaaaatat aaatggacgg atttattttg gattttattt
     5881 gttattttga cctgcctcta tattggtaac catgatttgt ttactctcaa tcatcaagaa
     5941 ttctcttttc gtggtagcgt ttggggtctg gtactggcct tatatcactt actattcatt
     6001 gataagtttg ttatatcgaa tcgaaaataa agattagagc tatgcttgac tgtgtacttt
     6061 taggatttat tttggaggaa gattttgtct ctattattta ttattttaaa tttatttatt
     6121 ttgtataaga tctattcttt gaggcgggaa aaatcgaaat acttgattta tacggcctat
     6181 atcatatttg gggtaaatgt agtatatggt attcaatggt tattaaaaga actgatttca
     6241 actatttccc cttaatgtgt atggaaacta taacaaaacg cataagatta aggtttgtgt
     6301 acctgttctt atacgttttt gtcatattga agattttcgg taagttttct acttttgcga
     6361 agtctagttt atactcaatg aaaatcaaag agtaaactag ccgtaggttg ctcaaagtac
     6421 agctttgagg ttgcagataa aactgacgaa gtcagctcaa aacatggttt tgaggttgta
     6481 gatgaaactg acgaagtcag ctcaaaacat ggttttgagg ttgtagatga aactgacgaa
     6541 gtcagctcaa aacatggttt tgaggttgta gatgaaactg acgaagtcag taaccatacg
     6601 tacggcaagg cgacgttgac gtggtttgaa gagtattaga atgctttccc aaccaaaatc
     6661 tctgcttcga tggtaatctc tgtcagtttg ctccagtcaa ttttgctctg gtcaacgtga
     6721 aagaggagag gagtcatact gagtagggct tggagttgtt ctgctgtgat agtcttagtc
     6781 agagaggcag tttgactaga taggatggta aagtgttcct ggaaatgctc cttgatatct
     6841 tggttagaat actccttgtt tgtcagctgg tcctgtactc tttgacggat ttctttgagg
     6901 tgattttcag ttgggataac ctttatcaag ataccgtctt tggataaaac gcgacgaaat
     6961 tctccatagt tggcaggtga gaagatatca agcagaatat ccatgttagc gtcttttata
     7021 ggaagtcgtg ccaagtcgcc aacgaaccaa ttgactgccc agttgggttc acttttagca
     7081 gcgatttgga ctgaatcttt ggagatgtca aaggcataga aagttttttc agagtgactt
     7141 tcttgtagtt tgcgagaata gaatccttca ccacaaccga tatccaaaat tgttgtggta
     7201 gtttttgagc ttgcaagcaa gtcagataca gcatctaaga tagcttggta aaagccggct
     7261 tctaggattt gttgacggtt ttgaaaattt tccttgtcgt agttagcaga ttgcttgatt
     7321 tgagggacta gattgacata gccaaatttc gccaagtcaa aagaatgacg gttgcagcac
     7381 ttgaaattag tctctaacag agtcagattt tcttgacaga taggacaggc aaaggcagtc
     7441 gcagaagcaa aacgctgaag tttgggcttg agatttgtat tcatagttta agtgtatcaa
     7501 aagaggcaaa tgaaagcaac tttaggaata agtagatggg agattcagta gaaataaaac
     7561 taattctcca atttatagaa tgaaattgct tttatatcta aattgctata taataatgat
     7621 aaggaggaaa taagtatgtt aaaagaagta ttaaccgttg caaaagttgc gaaaaaatct
     7681 tcattatttt tgggtggtgt cgcatttggt acccttggtt tgaaaatctt agcaagtaag
     7741 gaagctaaaa aaggttattc taaagctttg gctaaggctt acaagttgaa agacgagcta
     7801 gatgcatctg tttctgttgt gaagcaacat ggagacgatg tcttgcaaga tgccaaatat
     7861 ttgtacgagc aagagaaaaa agaagagcaa ttagatagcc ttataggtga ataatatgtc
     7921 ttttaaagtg ctacatagag gataccaaca tatccgacta tcatcttctt tttcacttac
     7981 cttggatatt caagactatc ttcgttcctt ggcgagagat gaaaagggga ttgagtctat
     8041 ccagttttac atggatcaac agcactttac tctacgcata aaagaaggct tttctgtatt
     8101 agataatgca gaagcctttt taaaaagaat tgataaaggg aaagtttctg agttgatgac
     8161 tcttcccatt cgtagagaag agagtgctta ttctattgtt tcaggtgcag cggttaagcg
     8221 tgtacttttt cgtagttttg tgccgtatcc tattcgctat atatggactt gttatcaggc
     8281 tttgggttat attagagaag cctatcaaac actagcgcgt aaggaactaa cgatggaagt
     8341 cttggactgt tcggctattt tattgtcttt gtttatgaac caatccaaga cagctagcaa
     8401 tatcatgttt atgcttgatt tggggaatca tttagatcag tggtccttga aaaaaactgc
     8461 aacagattta gaacagagtc ttcttgcaaa agagagcgat gtattcctag tacagggcga
     8521 tacggttgtt agtatcaaga gttccgatgt tcaaatagga gatgtcttga tcttatctca
     8581 aggaaatgaa attctgtttg atggacaagt agtttcaggt ttaggtatgg tcaacgaaag
     8641 ttccttgaca ggagagagtt ttccagttga aaaaagagag tctgatttgg tttgtgcaaa
     8701 tacagtatta gaaactggag agttacgcat tcgtgtaaca gataatcaga tgaacagccg
     8761 tattttacag ctgattgagt tgatgaagaa atctgaagaa aacaagaaaa cgaaacaacg
     8821 ctatttcatc aagatggcgg ataaagtcgt caaatataat ttcttggggg ctgggctgac
     8881 ttacctattg acaggttctt tttctaaggc tatttctttc ctattggtcg atttctcctg
     8941 cgctttgaaa atctctactc ctgtagctta tttgacagtt atcaaggaag ggttgaaccg
     9001 tgaaatggtg attaaggatg gagatgttct ggagaaatat ctggaagttg atactttctt
     9061 gtttgataag acaggaacaa tcacaactag ttatcctata gttgaaaagg tgttaccttt
     9121 tggagactat agtgaggaag atattctcag aatcagtgcc tgtcttgagg aacacattta
     9181 tcatcctatt gctaatgcca tcgtcaagca agctgagata gagggaattg aacatgagga
     9241 aatgcatggg aaactccaat atatcgcaag caaggggatc aaatctcata tagatggcca
     9301 accagttctt attgggaatt atgtcttgat gcaggatgag cagattcata tcagttcaga
     9361 acaaaatgct ttaattgaag agtacaagag tcactacaat ctcttattct tggcttatca
     9421 aaatgaattg attggaatgt tctgcattca tactcctttg agaaaagaag caaaaacagc
     9481 cttggataaa cttaaggcac aggggaaaaa attgattctg gcaacaggag acaccttgat
     9541 taggacagag gaattagtca aagatttgcc ctttgatcag gtctatacag acttgaaacc
     9601 tgatgggaaa tttgagttag tagagaaact gcagaaagca ggtcacacta ttttgatggt
     9661 tggagatgga ttgaatgact cagcggcttt aactctatca gatatcggtg tggtgatgaa
     9721 tgagagtgca gatatttcta agcagatgag tgatatctta ttgttagata atcgtttgga
     9781 tttcttccaa gagttggatt cgttatcatc atctttgcaa acactcatca agaaaaatat
     9841 tcaggatacc gttgtcgtaa atagtagttt gattggcttt ggcttattta attggttcag
     9901 tccttcaaat ctctctatct tacataatct aacaaccttg cgcattgtcc tgcgtagcct
     9961 gtctattaaa aatagataga tgagagttat taacagcaaa aaggagttac ctttctcgag
    10021 gttaactcct ttgtttatag ataaatgttg aatagtttag taagtcaata caaagtattt
    10081 tttcttccca cgacggataa cagtcagttc attctctaac ttatcagcgt cactcaagac
    10141 atagtcaagc tcttggatgc ggtcgccgtt tacgtagatg gctccgtttt ggacgtcttc
    10201 acgggcttgg cgttttgagt taactatacc agatgagacg agcagttcca cgatattgtt
    10261 gttttcgtct gcctgtactt ggtagttggg cacaccacga agtccttgtt tgagctcttt
    10321 gacagaaagg tttttgatgt ttcctgcaaa gagttgctca gtgatgttaa gtgcttcttt
    10381 gtaggcttct tctccgtgaa caagtgtaac aacttcacga gccaagactt tttgagccaa
    10441 gcgttcgtgt ggcgctgctt caaattgttt acgaatatct tcaatctcat caagtgacaa
    10501 gaaagtaaag attttcaaga agcgaacagc gtcagcgtcc atcacgttca tccagaattg
    10561 gtacatttcg tatggagaag tcttttcggg attgagccag acggcatttc cttctgattt
    10621 accaaatttc ttaccagttg catctgtgat tagtggaaca gtgataacgt gaccagtctt
    10681 gtccgcctta cgacgaagca attcggtacc agctgtcata tttccccact ggtcagaacc
    10741 accgatttga agagtgacat tatggtcttg gttaaggacg aagaagtcat acccttgcat
    10801 gatttggtaa gcgaactcag tgtaagaaat tcctgtttcg atccgttttt taacagattc
    10861 cttactcatc atgtagttga ccgtgaagta ttttccaata tcacggagga agtcaatgaa
    10921 gctgatgctg ccaaaccagt cgtagttgtt gaccatgaca gccttgtttt cgccattttc
    10981 aaagtcaaga aaacgagaaa gttgtccttg gatagacttg acccagccat ctactgtgtc
    11041 ttttgtttgg agactacgtt cagcatcttt gaaggacgga tctccgatga gacctgtagc
    11101 accgccaacg agcgcataag gtttgtgacc tgctagttgc aagcgacgac ttgtcaagat
    11161 tgcgacaagg tggcctaggt gaaggctgtc agcagttgga tcgtagccag tataataaga
    11221 aacttgacct tcttctaggg ctttacgcaa agcttcttca tcagtcgttt gaaatatcaa
    11281 accacgctct tttagctcat caaaaatgtg catgtgtttt ttctcctttt taaaattatt
    11341 gtttctacct attgtatcac aaacttgggc aatagcctag cagaataggg aagaaagtgg
    11401 ctattttatt gaaaatttcc cttttgtggt ataatagata aagtgaggaa atccatgcaa
    11461 aatcaattaa atgaattaaa acgaaaaatg ctggaatttt tccagcaaaa acaaaaaaat
    11521 aaaaaatcag ctagacctgg caagaaaggt tcaagtacca aaaaatctaa gaccttagat
    11581 aagtcagtca ttttcccagc tattttactg agtataaaag ccttatttaa cttactcttt
    11641 gtactcggtt ttctaggagg aatgttggga gctgggattg ctttgggata cggagtggcc
    11701 ttatttgaca aggttcgggt gcctcagaca gaagaattgg tgaatcaggt caaggacatc
    11761 tcttctattt cagagattac ctattcggac gggacggtga ttgcttccat agagagtgat
    11821 ttgttgcgca cttctatctc atctgagcaa atttcggaaa atctgaagaa ggctatcatt
    11881 gcgacagaag atgaacactt taaagaacat aagggtgtag tacccaaggc ggtgattcgt
    11941 gcgaccttgg ggaaatttgt aggtttgggt tcctctagtg ggggttcaac cttgacccag
    12001 caactaatta aacagcaggt ggttggggat gcgccgacct tggctcgtaa ggcggcagag
    12061 attgtggatg ctcttgcctt ggaacgcgcc atgaataaag atgagatttt aacgacctat
    12121 ctcaatgtgg ctccctttgg ccgaaataat aagggacaga atattgcagg ggctcggcaa
    12181 gcagctgagg gaattttcgg tgtagatgcc agtcagttga ctgttcctca agcagcattt
    12241 ttagcaggac ttccacagag tcccattact tactctcctt atgaaaatac tggggagttg
    12301 aagagtgatg aagacctaga aattggctta agacgggcta aggcagttct ttacagtatg
    12361 tatcgtacag gtgcattaag caaagacgag tattctcagt acaaggatta tgaccttaaa
    12421 caggactttt taccatcggg cacggttaca ggaatttcac gagactattt atactttaca
    12481 actttggcag aagctcaaga acgtatgtat gactatctag ctcagagaga caatgtctcc
    12541 gctaaggagt tgaaaaatga ggcaactcag aagttttatc gagatttggc agccaaggaa
    12601 attgaaaatg gtggttataa gattactact accatagatc agaaaattca ttctgccatg
    12661 caaagtgcgg ttgctgatta tggctatctt ttagacgatg gaacaggtcg tgtagaagta
    12721 gggaatgtct tgatggataa ccaaacaggt gctattctag gctttgtagg tggtcgtaat
    12781 tatcaagaaa atcaaaataa tcatgccttt gataccaaac gttcgccagc ttctactacc
    12841 aagcccttgc tggcctacgg tattgctatt gaccagggct tgatgggaag tgaaacgatt
    12901 ctatctaact atccaacaaa ctttgctaat ggcaatccga ttatgtatgc taatagcaag
    12961 ggaacaggaa tgatgacctt gggagaagct ctgaactatt catggaatat ccctgcttac
    13021 tggacctatc gtatgctccg tgaaaagggt gttgatgtca agggttatat ggaaaagatg
    13081 ggttacgaga ttcctgagta cggtattgag agcttgccaa tgggtggtgg tattgaagtc
    13141 acagttgccc agcataccaa tggctatcag accttagcta ataatggagt ttatcatcag
    13201 aagcatgtga tttcaaagat tgaagcagca gatggtagag tggtgtatga gtatcaggat
    13261 aaaccggttc aagtctattc aaaagctact gcgacgatta tgcagggatt gctacgagaa
    13321 gttctatcct ctcgtgtgac aacaaccttc aagtctaacc tgacttcttt aaatcctact
    13381 ctggctaatg cagattggat tgggaagact ggtacaacca accaagacga aaatatgtgg
    13441 ctcatgcttt cgacacctag attaacccta ggtggctgga ttgggcatga tgataatcat
    13501 tcattgtcac gtagagcagg ttattctaat aactctaatt acatggctca tctggtaaat
    13561 gcgattcagc aagcttcccc aagcatttgg gggaacgagc gctttgcttt agatcctagt
    13621 gtagtgaaat cggaagtctt gaaatcaaca ggtcaaaaac cagagaaggt ttctgttgaa
    13681 ggaaaagaag tagaggtcac aggttcgact gttaccagct attgggctaa taagtcagga
    13741 gcgccagcga caagttatcg ctttgctatt ggcggaagtg atgcggatta tcagaatgct
    13801 tggtctagta ttgtggggag tctaccaact ccatccagct ccagcagttc aagtagtagt
    13861 tctagcgata gcagtaactc aagtactaca cgaccttctt cttcaagggc gagacgataa
    13921 ttctctaaat gaagtggcca atcaatggat tgccactttt ttcctttcgt gttacaataa
    13981 gggtatgaat cagtatcaga aaaagattgt taacggaaaa atttattcgc tcctatccgg
    14041 cttaatatgg ggaatctgtg gaattttagg agagtacttc tttactcatt atcaggtgtc
    14101 ttcgggctgg attacctcta tgcgtttgac actggcaggg agtcttgtac tcatttggtc
    14161 tgcaatacaa ttaaaatcgc aagtgctaga tatttggcga gacaagaaaa attacctgcc
    14221 ctttttagcc tatgctattt tggggatttt ttcagttcag tattttttct atctctgtgt
    14281 agaatactca aatgctacga cagcaactat tttacagttt attagccctg tctttatcct
    14341 cttttacaat cgcttggttt atcaaaaacg agcgtcaaaa agcgctgttt tctatgtttt
    14401 ggttgccatg ctgggtgttt gcttgatggc gacaaaggga gacctctctc agttatccat
    14461 gacgccgcta gctcttataa caggtttgct gagtgccatg ggtgttatgt ttaatgttat
    14521 cttgccccaa ccttttgcta agcgttatgg ttttgttcct acggttgggt gggggatgat
    14581 tttggcaggt ttgtttagca atgtcctctc gccggtttat cagctttcct ttactcttga
    14641 tatttggagt atcttgattt gcctcattat cgctttcttt ggaacggctt ttgctttttt
    14701 catttccatg aaggctgtgt ccttggtttc tcctttggtg gttgccgtta tcagtgccag
    14761 tgaacctctc tcttctgctc tcttgagtgt tttgttctta ggattagtag tggattggtc
    14821 cctccttcta gctatagcct tgattatttt acccatgata tttttgtcta tagaagaagc
    14881 gaaagaaagt agataaaaag tcttttctta attctaaaag tgtgctatac tggaatagtc
    14941 aataaaacac ggggagatag ttgtgaagag tgtttttagg ttgtatcggt aggggcttgc
    15001 ttttaccgac agcacgtttt atctcacatc cctaaaaaat catacctaaa aacagacaaa
    15061 aaggtttcaa atttgaggcc ttttttggta gaataggtat cattataacg aaccaggagg
    15121 cacctatgac tgctacaaaa atgaacgctc aagaaattat ccaatttatc gccaatgctg
    15181 aaaagaaaac cagtgtcaaa gtaacctttg aggggcaact cgcaactgct gtgcctagct
    15241 ctgttgtcaa actaggaaat gtcctattcg gagactggaa ggatgtggct ccgcttcttg
    15301 aaggtttggt agaaaatcaa gattatgttg tcgagcaaga tgctcgtaat tctgcagttc
    15361 ctttgctaga taagcgtgct atcaacgctc gtatcgagcc aggtgcgatt atccgtgacc
    15421 aggtggaaat tggtgacaat gctgttatca tgatgggatc tgttatcaat atcggtgctg
    15481 aaatcggtgc tggaaccatg attgacatgg gtgccatcct tggtggccgt gccatcgttg
    15541 gaaaaaatag ccacgttggt gcaggtgcag ttttggcagg tgtgattgag ccagctagtg
    15601 ctgaaccagt ccgtgtcgga gacaatgttc ttatcggtgc taatgcagtg gttatcgaag
    15661 gagtccaaat cggtagtggt tcagttgtcg cagcaggagc tattgttacc caagatgtcc
    15721 cagaaaacgt ggtagtagca ggtgttccag ctcgtattat caaagaaatt gatgcccaaa
    15781 ctcaacaaaa aacagcgcta gaggatgcgc ttcgtacctt gtaattgtaa aagtaaaaaa
    15841 gaggcggaac cctttttcca gcctctttct gctatatcgg aggacagata gatgttagat
    15901 ttgattcaga ctagacgaga tttacaccag attccagaga ttggcttgga ggagttcaag
    15961 actcaggctt atttgctgga tgtgattgag aaattgacta cgggcaagga ttttgttcaa
    16021 attcgtactt ggcggacagg gattttggtc tacctgcagg gaagtcagcc ggagcgaacc
    16081 attggttggc ggacagatat tgatggcctg cctatcgtcg aacaaacagg tctacctttt
    16141 gcttctcaac accaaggtcg catgcatgcc tgtggccatg attttcatat gaccattgcc
    16201 ttaggctgtc ttgagcgcgc ccttgaggag caacccaaga ataatctgct ctttctattt
    16261 cagcctgctg aagaaaatga agctggtggt atgctcatgt atgaggacgg tgcttttgga
    16321 gattggttgc cagatcaatt ctatggtctc catgttcgtc cagatttgaa agtcggccag
    16381 attgcgacta atactcatac actctttgca ggaacttgcg aggtgaagat ccgtttcaaa
    16441 ggcaaaggtg gccacgcagc ttttcctcat gaagccaatg acgccttggt ggcggctagt
    16501 tactttgtga cccaagtgca gtcagttgtt agccgcaatg tcaacccaat cgagggagca
    16561 gtggtgacct tcggcgtttt tcaagctgga accaccaaca atgtcatcac agacacagct
    16621 tttttacatg gaactattcg tgccttgaca caggacatga gtctcttggt gcaaaagagg
    16681 gtaaaaacag ttgcagaagg ggttgcagct gcctttgata tggaagtcga agtggaactc
    16741 aaacaaggtg gatacctacc tgttgagaac aatccagcct tggcgcgtga actgatggac
    16801 ttctttgacg aaaaagacgg aatcgagttg attgatatcg aacctgctat gacaggcgag
    16861 gactttggtt atctcctttc aaaggttgat ggcgtgatgt tttggctagg tatcgatagt
    16921 ccctacgctc ttcatcaccc tcaaatgagt cctaaggaag aagtcttagc tattggggtg
    16981 gctgcggtgt ctagtttcct gaaaaagaag gcagcagaat agagggcttg tctatgaaat
    17041 cggaattacg caagcaagtc ttgcatgaaa tgaaggctat atctcaagag caaaaacagg
    17101 ctatagacca agctttaacc gagcggcttt tacaacaccc cttttatcaa gaagccaagg
    17161 tcatcgcaac ctacctctct ttttctcatg agtttcaaac gcaggaactg attgagcagg
    17221 cgctgaagga cggcaagaag gttttaatac ccaaaactta tcccaagggg cgcatggact
    17281 ttgtggtcta tgatccgcaa cagttggtaa aaactttctt tgccttactg gagctacagg
    17341 gagatttgga agtggtggat gcatctcaga ttgatttgat tcatgttcct ggtctggctt
    17401 ttacgacgaa aggatatcgg ataggatacg gtggaggcta ttatgaccgc tatctggaac
    17461 atttttctgg tcgtactttg agtacgatat atccttgtca aattcaggac tttatacctg
    17521 aaaaccatga tattcctgtt caggaggtat taattgatga aggaaatctt tgatagacgt
    17581 taccctgtga cgagtttctt cctcttagtg acggccttgg tatttttact aatgttggtc
    17641 actgcaggcg gaaactttga cagggcagat acattatttc gatttggagc catgtatggg
    17701 ccagctattc gcctctttcc cgagcaggtt tggcgtctct tgtctgccat ttttgttcat
    17761 attgggtggg aacatttcat tgttaatatg ctttcacttt attatcttgg aaggcaggta
    17821 gaggagattt tcggttctaa gcagtttttc tttctctatc ttttatcagg aatgatgggc
    17881 aatctctttg tttttgtatt tagtcctaaa tccttagcag caggcgcctc tacctctctt
    17941 tatgggctat ttgccgcgat tattgttctt cgctatgcaa ctcgcaatcc ttatatccaa
    18001 cagctagggc aatcctatct gacacttttt gtggttaaca ttattggaag tgttctgatt
    18061 ccaggaatca gcctagcagg ccatatcggt ggtgcagttg gtggcgcatt tctagcagtt
    18121 atctttccag ttagaggaga aaaacggatg tataacacca gccagagatt aggagcggta
    18181 gtcttgttcg taggactcgc cattttgctt ttctacaagg gaatgggatt gtgacgacta
    18241 atactcaatg aaaatcaaag agcaaactag gaagctagcc gcaggttgct caaagcactg
    18301 ctttgaggtt gtagatagaa ctgacgaagt cagctcaaaa catggttttg aggttgtaga
    18361 tgaaactgac gaagtcagta accataccta cggtaaggcg atgctgacgt ggtttgaatt
    18421 tgattttcat tgagtataat atgtaaactt gaataagaaa aaagctactt aaaaaatgag
    18481 tagcttttaa tgtgttttgg aaccattcga aacaacacag ctctaaaata gttattagcg
    18541 ttgactaggt tctgcaatgc cgccaccaaa gttcccaagg taatttccat cgtcatcata
    18601 cacatctcca ttattatgat ctttgtagta gtatctttga tagccgtcag caccagtgcc
    18661 tttatatctg tagacattat ttgctccgct gtttgaattg ctagatgaat attgttctga
    18721 tgatccacca tttgttgaac tatctcctga accagaataa tcttgaccgc cataattatt
    18781 tgaatatcca ttagtttgtt cagatgaact ataatctaca tttgaattgg aagatccatt
    18841 ataagactca ttagaagcac taccagaaga attactttga ctagaagatt cctttgcttt
    18901 ttcagcagct ttttcagcag ctttttcttc ttctttcttc aaaagttcga tttgtttttg
    18961 atatttatca acaagatcat ttagagattt aagagtttct tccaaattct cttgtttaaa
    19021 agtttcatca acttttttga taaaatcaat ttgttttgta atttctttta catttgtttc
    19081 aagagttgct ttatctttaa atttgtcatc tgaatcttta acatcataag ccaaaccttt
    19141 ttgcaaatga aaatctacaa ttgctttaga tgattctttg actttgttat taagatagtc
    19201 tttacctgca tcggttttaa gtgaagtttc agatagttct tttaatttag aaatagattc
    19261 atctgaagat gcatcaaatg attcaaatag tttatcaact tgttctttgt ctgatttgct
    19321 atctttttct aattgtacaa tttttgcttg ttgttctgct tgttgttggt tatatgaata
    19381 ccatccaaac cctaatacca atacaacacc aactgcaact gatgaaatga ttactttttt
    19441 attgttttcg ataaattcta caaatttgtt ctccataatc tttttctttg atagtgtccg
    19501 caattacttt aaaattaaaa ttacacctat ctttctccct tattatattt ttattaatag
    19561 cttccacaaa attaaacaac ttatgttcgg ctcggttgat tt
//
LOCUS       DAVHEX010000047        19412 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVHEX010000047 DAVHEX010000000
VERSION     DAVHEX010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19412
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.047"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            13..393
                     /locus_tag="VVL45_001565"
     CDS             13..393
                     /locus_tag="VVL45_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187721.1"
                     /translation="MKLKRLALISGIVGLVGGILLLIGPFVLLGTAVNTAATTLNGGA
                     TAGAFSGVALLLNALKIANLVLGIIAIVYYKGDKRVGAAPSVLMIVSGGVSLIPFLGW
                     VGGILAIIGGSLFLATLKKFKSEE"
     gene            928..1620
                     /locus_tag="VVL45_001566"
     CDS             928..1620
                     /locus_tag="VVL45_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEU4187722.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIKFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            1673..4084
                     /locus_tag="VVL45_001567"
     CDS             1673..4084
                     /locus_tag="VVL45_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEU4187723.1"
                     /translation="MIRNKKQDYVLAYKQPASTTYMGWEEEALPIGNGSLGAKVFGLI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYGFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFNQQGTTLSQVTDYQRQLNISKALATTSYVYKGTRFE
                     REAFASFPDDLLVQCFTKEGLETLDFTIELSLTCDLASDGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEADVDASTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDYPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQLNYWPAYVTNLLETVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSY
                     KGQEYIEAARAGLNDRGDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKISTLPNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            4334..5035
                     /locus_tag="VVL45_001568"
     CDS             4334..5035
                     /locus_tag="VVL45_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4187724.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIQMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            5037..7745
                     /locus_tag="VVL45_001569"
     CDS             5037..7745
                     /locus_tag="VVL45_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU4187725.1"
                     /translation="MIKRKTYWKDLIQSFTGSKGRFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDKEIALTTHLQDQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEELEQILSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQVQREQASAQLTQAKQELGKEEAKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAA
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIIAKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGMVVGETQIQFYWTYSLLAFVLSLLASVLPAYLVAWRELHDEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATNQDKVELAEVLKGQEILAYQKIYSKTL
                     DKDFKGKAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMNQSAVSSVVQNASAIRLFDSIASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTIKVLGFHNNEVTLYIYRETIVLSLVGIVLGLIAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVAAVSFILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(7841..8332)
                     /gene="tpx"
                     /locus_tag="VVL45_001570"
     CDS             complement(7841..8332)
                     /gene="tpx"
                     /locus_tag="VVL45_001570"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEU4187726.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGHDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(8455..9384)
                     /gene="psaA"
                     /locus_tag="VVL45_001571"
     CDS             complement(8455..9384)
                     /gene="psaA"
                     /locus_tag="VVL45_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HEU4187727.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            complement(9416..10264)
                     /locus_tag="VVL45_001572"
     CDS             complement(9416..10264)
                     /locus_tag="VVL45_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU4187728.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            complement(10261..10983)
                     /locus_tag="VVL45_001573"
     CDS             complement(10261..10983)
                     /locus_tag="VVL45_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU4187729.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
     gene            11274..13166
                     /gene="pepO"
                     /locus_tag="VVL45_001574"
     CDS             11274..13166
                     /gene="pepO"
                     /locus_tag="VVL45_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEU4187730.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRSEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
     gene            complement(13289..13918)
                     /locus_tag="VVL45_001575"
     CDS             complement(13289..13918)
                     /locus_tag="VVL45_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU4187731.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            complement(14258..14500)
                     /locus_tag="VVL45_001576"
     CDS             complement(14258..14500)
                     /locus_tag="VVL45_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187732.1"
                     /translation="MTRFEEIFEEYKNPQDTFFYPLVYKENTYEKTAISIFALLMLGV
                     CCLFLFSQQSYKKLVQYYANDQNLPSRITYSEYSDK"
     gene            14911..17133
                     /locus_tag="VVL45_001577"
     CDS             14911..17133
                     /locus_tag="VVL45_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEU4187733.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRMIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            17163..17606
                     /locus_tag="VVL45_001578"
     CDS             17163..17606
                     /locus_tag="VVL45_001578"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEU4187734.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKRGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(17873..18058)
                     /locus_tag="VVL45_001579"
     CDS             complement(17873..18058)
                     /locus_tag="VVL45_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187735.1"
                     /translation="MKKIIFIKTIQLLVIDGIMLAFLTFKRGLTWDWILIYSGWLIFF
                     HPVLLTYLSNQLCDHFS"
     gene            complement(18077..18490)
                     /locus_tag="VVL45_001580"
     CDS             complement(18077..18490)
                     /locus_tag="VVL45_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187736.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFITRLETSFEWLLLSGLIFFLA
                     QGLLLFLLVVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMP
                     IITACHSTFYYMTADYLRENYPDFYDKHISLWECL"
     gene            complement(18621..19268)
                     /locus_tag="VVL45_001581"
     CDS             complement(18621..19268)
                     /locus_tag="VVL45_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEU4187737.1"
                     /translation="MVVGIAIASGYLNSHLDKFVDWGSWTALVPFGLISVTNVGISML
                     STRFTGKLSKWGNYFGIVNTILSGAIDYILGNKAAIITYPVTFLIYTFAIKKWEASQE
                     GRPNQMSQKQVKLAAIIISIIAFLFAFVTNYIGYGGKMNLLAYVTTIAFALSLIANAL
                     NALKLTTQWGFWLIYNFVQLTKAGIQGNFANIGKYIFYILNAIGALFVWNDEEVR"
BASE COUNT         5816 a         3653 c         4436 g         5507 t
ORIGIN      
        1 ggagatttta ttatgaaatt aaaaagactt gctttaatta gtggtatcgt cggtcttgtg
       61 ggaggaattt tacttcttat tggtcctttt gtcttgttgg gaacagcggt aaacacagct
      121 gctacaactc ttaatggagg agctactgca ggggcttttt caggtgttgc cttactcttg
      181 aatgccttga agattgcaaa tcttgttctt ggtatcattg ctattgttta ctataaagga
      241 gataagcgtg taggtgcagc tccgtctgta ctaatgattg tttctggtgg agttagtctc
      301 attccattct taggatgggt tggggggatt cttgctatta tcggaggatc tctattcctt
      361 gcaacattga agaaattcaa atcagaagaa taaaaggtat tttagcatga aaagaacaaa
      421 aaagtttatc ggtataggag tagctctatt atctctttct tttctagttg catgtggaac
      481 ataaagttca aagaatactt caacaagtaa tgatgagaag acagtagcaa catccaatag
      541 ttcaaaagaa acaatcactt tcgatacacc ggttgtaaca gacgatgcga ttgaatcaat
      601 acgcacttat gcagattata tagatcttta taaaaatatt tttgatgatt attttactaa
      661 agctgaggaa ggtttcaaag gcatagctat ggaaaataat gactcgttta ctaaactaaa
      721 agagtcaact caaaaattat tcgatgcgca gaaaaaaagg ttaaataatg aagatagaat
      781 agaaacaacc aaaaacaatg tgattgccaa acattgtcaa acagtccttt cctttttggt
      841 tttgactagc ttttttgtga aaaattgtgt aaaatagaat agataaacga ggggaaacct
      901 cggaaaattt aaaggagaat ccatctaatg gtaaaattgg tttttgctcg ccacggtgag
      961 tctgaatgga acaaagctaa ccttttcact ggttgggctg atgttgattt gtctgaaaaa
     1021 ggtacacaac aagcgattga cgctggtaaa ttgatcaaag aagctggtat caaatttgac
     1081 caagcttaca cttcagtatt gaaacgtgct atcaaaacaa ctaacttggc tcttgaagct
     1141 tctgaccaat tgtgggttcc agttgaaaaa tcatggcgct tgaacgaacg tcactacggt
     1201 ggtttgactg gtaaaaacaa agctgaagct gctgaacaat ttggtgatga gcaagttcac
     1261 atctggcgtc gttcatacga tgtattgcct ccaaacatgg accgtgatga tgagcactca
     1321 gctcacacag accgtcgtta cgcttcactt gacgactcag ttatcccaga tgctgaaaac
     1381 ttgaaagtga ctttggaacg tgctcttcca ttctgggaag ataaaatcgc tccagctctt
     1441 aaagatggta aaaacgtatt cgtaggagct cacggtaact caatccgtgc ccttgtaaaa
     1501 cacatcaaag gtttgtcaga tgatgagatc atggacgtgg aaatccctaa cttcccacca
     1561 ttggtattcg aattcgacga aaaattgaac gttgtttctg aatactacct tggaaaataa
     1621 aaaattgtaa gcctaggatt gatttctagg ctttttatgt tagtatggaa gtatgataag
     1681 gaataaaaaa caagattatg tactggccta caagcaacca gcttcaacca cttacatggg
     1741 ttgggaagaa gaagctttac cgataggcaa tggttcttta ggagcaaaag tatttggcct
     1801 tataggggct gaacggattc aatttaatga aaaaagtctc tggtctggag gtccacttcc
     1861 tgatagttca gattatcagg gtggaaatct tcaggatcag tatggttttt tagctgagat
     1921 tcggcaggct ttggagaaga gagattacaa tctggctaag gaactggctg agcagcacct
     1981 aattgggcca aaaacgagtc aatatgggac ctatctgtct tttggggata ttcacattga
     2041 gttcaaccag caaggtacga ctttgtctca ggtgacggac tatcagagac agttgaatat
     2101 tagtaaggca cttgcgacga cttcttatgt ctataaggga acgcgatttg aacgtgaagc
     2161 ttttgcgagt tttccagatg atctcttggt tcaatgtttt actaaggaag ggttggaaac
     2221 tctagatttt actatagaac tatccttgac ctgtgatttg gcttctgatg gaaagtatga
     2281 gcaggaaaaa tctgattaca aggagtgtaa gttggatatt actgattctc atatcttgat
     2341 gaagggaaga gttaaggata atgatctgcg gtttgctagt tatctagctt gggaaacgga
     2401 tggagatatt agagtttggt cagatagggt tcagatatca ggagccagtt atgccaatct
     2461 cttcttggcc gctaagacgg attttgccca aaatcctgct agcaattatc gcaagaaact
     2521 agatttagag caacaggtga tagacttggt ggacacagct aaagaaaagg gctataccca
     2581 attgaaatca aggcatatcg aggactacca agccttattc cagcgtgttc aattggattt
     2641 ggaagctgat gttgacgcat ccactacaga tgatttgtta aaaaattata agccacaaga
     2701 agggcaggct ttggaggagc tgttcttcca gtatggacgg tatttattga ttagttcgtc
     2761 cagagactac ccagatgctc taccagctaa cctacaggga gtctggaatg cggtcgacaa
     2821 tcctccttgg aattcggact atcacttaaa tgtcaatctg cagctgaatt attggccagc
     2881 ctatgttacc aatctcctag agacggtctt tccagtcatc aactatgtag atgatttgcg
     2941 tgtctatggt cgtctagcgg ctgtaaagta tgcaggaatc gtctctcaaa aaggtgagga
     3001 aaatggttgg ttggttcata ctcaagcgac tccctttggt tggacggcac ctggttggga
     3061 ttactattgg ggttggtcac cagctgccaa tgcgtggatg atgcaaaccg tttatgaagc
     3121 ctattcattt tatagggacc aagactatct cagggagaaa atttatccca tgttgaggga
     3181 aacggttcgt ttttggaatg cctttttaca taaggatcag caggcgcagc gttgggtctc
     3241 ttctccgtct tattccccag aacatgggcc gatttcgatt ggcaatacct atgaccaatc
     3301 tctgatttgg cagttatttc atgattttat tcaggctgct caggaattgg gactggatga
     3361 ggacttgttg actgaggtta aggagaagtc tgatttacta aatcctttgc aaatcactca
     3421 atctggtcga atcagggagt ggtatgagga ggaagagcag tattttcaaa atgagaaagt
     3481 ggaggcccag catcggcacg cttcccatct agtgggactc tatcctggca atctctttag
     3541 ctacaaggga caagagtata ttgaagcggc acgtgctggc ctcaatgatc gtggagatgg
     3601 cggcacaggc tggtccaagg ctaataagat caatctctgg gcgcgtttgg gagatggcaa
     3661 tcgagcccat aaattattgg cagagcagtt aaaaatatcc accttgccaa atctttggtg
     3721 tagccatcct ccttttcaga tagatggtaa ttttggtgct actagtggca tggcagaaat
     3781 gttactccag tctcatgcag cttatctggt acctctagct gccctacctg atgcttggtc
     3841 aacaggttct gtttcaggct taatggcacg tggacatttt gaagtgagca tgagctggga
     3901 agataaaaaa ctcttacagt tgaccatttt atcaaggagt ggaggagatt tgcgagtttc
     3961 ttatccagat attgagaaga gtgtgattaa aatgaatcaa gaaaaaataa aagcgaaatg
     4021 catggggaaa gattgtattt cggtggcaac agcagaaggt gatcttgttc aattttattt
     4081 ttaagaagat gttataaggc agtaatttga aacttccttt taataaggat ttaagaatat
     4141 aggcagtttc caactagttg aaaaagcgtt ataatgataa taggaagtaa tactcaatga
     4201 aaatcaaaga gcacaaacta ggaagctagc cgcaggttgc tcaaaacagt gttttgaggt
     4261 tgtagatgga agctgacgtg gtttgaagag agattttcga ggagtataat ttgtttgata
     4321 gagggtggat ctgatggctt atattgagat gaaacactgt tacaagcgtt atcaggttgg
     4381 ggacacggag attgtggcca atcgtgatgt gaattttgag attgaaaagg gggagctggt
     4441 tattatcctt ggtgcttcag gtgcaggcaa gtcaacagtt cttaaccttc ttgggggaat
     4501 ggataccaat gatgaagggg aaatctggat tgatggtgtt aatattgcgg attatagttc
     4561 ccaccagcgc accaattacc gtagaaatga tgtggggttt gtttttcagt tttataatct
     4621 agtttctaat ctgacagcta aggaaaatgt ggaactggct tctgaaattg tgacagatgc
     4681 cttgaatcct gatcaggtct tgacagatgt aggtctggct catcgtctca ataactttcc
     4741 agcccagctt tctggagggg agcaacagcg agtctccatt gcacgcgcgg tagccaaaaa
     4801 tcctaaaatt ctcctttgtg atgaaccgac tggagccttg gattatcaga cgggcaagca
     4861 ggttttgaaa attctccaag acatgtctcg tcaaaaggga gcgacggtga tcatcgtgac
     4921 tcataatgga gctttggcgc ccattgctga tcgcgtgatt caaatgcacg atgccagtgt
     4981 caaggatgtg gtgctcaacc agcatcctca ggatattgac agtttggagt actagcatga
     5041 tcaagcgaaa aacttattgg aaggacttaa ttcagtcctt cacaggctcc aaggggcgtt
     5101 ttttatccat cttgatcctg atgatgttgg gatctctagc cttagtaggc ctcaaagtaa
     5161 ccagtcccaa catggaggcg acagctaatg cttatttaac aactgctcaa accttagatt
     5221 tggcagtcat gtctaactat ggcttggatc aagcagacca agaagaacta aaacagacgg
     5281 agggcgcaga ggtcgagttt ggctatttga cagatgtgac tatggataat gggcaggatg
     5341 ccattcggct gtactccaaa ccagagcgaa tttcaacctt tcagctaaga aagggacgac
     5401 ttcctcagtc agacaaggaa atcgctttga ccactcattt gcaagaccaa tacagcgtgg
     5461 gacaggagat tagttttaaa gaaaaagaag agggtcattc ctctttaaaa gaccatactt
     5521 ataccattac tggttttgtg gattcggctg aaatcctctc ccagcgagat atgggctacg
     5581 caggaagtgg aagtgggact ctgacagcct atggggtgat tttacctagt caatttgatc
     5641 agaaagtcta caatatagct cgtttgaaat atcaagattt agcaggttta aatgcctttt
     5701 catcagctta tgaagaaaaa tccaagcaac atcaggaaga gcttgaacaa attttatcag
     5761 ataatggcaa ggtacgtctg caacttttga aaaaagaagg acaagagtct ctagacaagg
     5821 ggcaagagac ccttgacaag gctcagacta atttgcagga aggcaagcgt cgtttagcag
     5881 ctgctcaagc tcgtatacag gctcaagaaa gtcaactagc cttgtttcct caagttcaga
     5941 gagagcaggc tagtgctcaa cttacccaag ccaagcagga attgggcaag gaagaggcca
     6001 aactaaagca agctgaacaa aatctagccc aagaaaagga aaaattagaa aaacatcagc
     6061 aagtcttgga cgatttggcg gagccaaggt atcaggtcta taatcgtcag accatgccag
     6121 gtggtcaggg ctatcttatg tatagcaatg cttcatccag tattcgagca gtgggcaata
     6181 tctttcctgt ggtactttat gccgtagcag ccatggtgac ctttacgacc atgactcgct
     6241 ttgtagacga agagcgaact catgcaggga tttttaaggc cttgggttac cgtagtaagg
     6301 atattatcgc caagtttctc ctttatggac tagtagctgg gactgtcgga acggctctag
     6361 gtagtatact tggtcattat ttgctagcca gtgtaatttc aagtgtcatt acaaaaggca
     6421 tggtggtggg agaaactcag attcagttct attggaccta tagcttacta gcttttgtct
     6481 tgagcttgtt ggcgagtgtg ttaccagcct atctggtggc ttggagggaa cttcatgacg
     6541 aagcagccca gcttctactt cctaaacctc ctgtcaaagg agctaaaatc ttattggagc
     6601 gtatcggttt tatctggcgt cgtctcagtt ttactcataa ggtaacagcc cgcaacatct
     6661 ttcgttataa gcagagaatg ttgatgacaa tctttggtgt ggcaggttct gtagctctgc
     6721 tctttgcagg tttgggaatc caatcttctg tagcaggagt tccgtctaaa cagtttcaac
     6781 aaatccaaca gtatcagatg cttgtctctg aaaatcctag tgcgaccaat caggacaagg
     6841 tagagctagc agaagtgttg aaagggcagg agatactagc ctaccagaaa atctattcta
     6901 aaacgctaga caaggatttc aaaggcaagg ctggtcttca aaacattact cttatgatga
     6961 tagagaagga agatttgact ccctttatcc atcttcaaca tcatcagcag gagctgacat
     7021 taaaagatgg catcgttatt acagctaaac tcgcccagct ggcaggtgtc aaggttgggc
     7081 agactttaga aattgaaggt aaggaactaa aggtcgctgc tattactgag aactacgttg
     7141 gtcactttat ttatatgagt caggctagct atgagcaact ttacggacag ctaccccaag
     7201 ccaacactta tctggtctca ttaagggata ccagtgcaac tagtatcgaa agtcaggcgg
     7261 gcttgcttat gaatcaatct gcggtgtcca gcgttgtcca aaatgcttca gccattcgac
     7321 tcttcgactc tatcgctagc tcactcaatc agaccatgac catcttggtc atcgtatcgg
     7381 ttctattagc tattgtcatc ctttacaatc tgaccaatat caacgtagct gagagaatcc
     7441 gtgaactctc cactatcaag gttcttggtt ttcataataa tgaagtcacc ctctacattt
     7501 accgtgagac gattgtgctg tcccttgtgg gaatcgtact tggtctgata gctggtttct
     7561 atttacacca atttttgatt caaatgattt cgcctgcgac tattctcttt tatccgcagg
     7621 taggctggga agtctatgta atcccagtgg cagcagtaag cttcattttg accttgcttg
     7681 gtttcttcgt caattattat ctgagaaagg ttgatatgtt agaagccctg aaatctgtag
     7741 agtaaggtag ttatttttag ctgattgaac ttctatttac taatattcaa aaatcctccg
     7801 tttcaaagag cagggaactc tttgtgacag aggatttttt ctatagggct ttagcagctg
     7861 caattgcggc ttcgaagttt ggctcagaat tgatattatc cacgtattca acgtagcgaa
     7921 tcgtattgtc agtatcgagg acaaagactg cgcgtgctaa taggtgccat tcgttgatca
     7981 agagggcata atcgtgcccg aaagaatggt caaagtagtc tgaaagcata atggcattgt
     8041 caaggccttc agcaccgcac caacgttttt gagcaaaagg taggtccatt gaaacagtca
     8101 atacgaccgt gttgtccagt ccagccaatt cttcattaaa acgacgtgtt tgagttgagc
     8161 agatgcctgt atcgatagaa ggaacgacac tcaagacttt tttcttgcca tcaaaatcag
     8221 ccagagattt tttagaaaga tctgttgtag taagagaaaa atcaagcgcc ttgtcgccga
     8281 cttgtagttg tttacctgta aagctcacag gatttccgag aaaagttacc ataggatact
     8341 ccaatctttt ttcttccatt gtatctgaaa cagtcagaat tttccaatga tttgaccgga
     8401 aatgtgggca tagaaaaaac gccagctcat atgagaatga cgtttttcag aggcttattt
     8461 tgccaatcct tcagcaatct tgtcaaggtt gtatttcatc atgctgtagt agctgtcgcc
     8521 ttctttacct tgttctgcga tagagtcagt aaagatttgt gcgtagattg ggatgtttgt
     8581 gtcttgagaa acggttttca ttggacggtc atccacactt gattctacaa agagtgatgg
     8641 aacttttgtt tggcgaagtt tttcaaccaa ggtcttgatt tgttcaggag ttccttcttc
     8701 ttcagtattg atttcccaga tgtaggcact tggaacacca taggctttag agaagtattt
     8761 gaatgctcct tcgctggtta caatgagttt cttttcagca gggatcttat taaatttatc
     8821 cttactttct ttatcaagtt tgtctaactt atcagtatat tctttgagat ttttttcata
     8881 gaattcttta ttgttagggt ctttggcgct caattgtttg gcgatatttt tagcaaaaat
     8941 aataccgttt tcaaggttaa gccaagcgtg tgggtcttct tttccttttt cattttgacc
     9001 ttcaaggtag ataacatcaa cgccgtcgct gactgcgaag tagtctttgt tttcagtttt
     9061 cttggcattt tctaccaatt ttgtaaacca agcattgcca cctgtttcaa ggttgatacc
     9121 gttatagaaa atcaaatcag cctcagaagt tttcttaacg tcttcaggaa gtggttcgta
     9181 ttcgtgtggg tcttgcccaa tcggaacgat actatgaagg tcaattttgt caccagcaat
     9241 atttttagta atatcagcga tgattgagtt tgtagcaaca acttttagtt tttgaccaga
     9301 agttgtatct ttttttccgc tagcacatgc tacaagaatg attgcagaaa gaaagagaac
     9361 gagtaatgta cctaattttt tcattagatc ctccaattta ttagggcctt gccccttatt
     9421 ttaacaaatg tttatttttc agtttcaaat atcgttgttt gggagcgata aagaagctaa
     9481 tgagaaagaa actagcagct gtaagcacga tactagaacc tgccgcaaca ttaaaactat
     9541 agccaataaa gagtcccaaa actgaagcag tagctccgaa ggttgaggaa aggaaaatca
     9601 tgcttttcag actattagca tacagataag cagttgcagc tggggtaatc agcatggcta
     9661 caatcaggat agttccgaca ctttgcatgg ctgtcacaga cacgagagtc aggagtacca
     9721 tgagaaggta gtgatagaaa ttgacaggca ttcccatggc tttagccaag agttcatcaa
     9781 aggaagttat caagagttgc ttgaagaaaa tccagattaa caagaggatg gctgccccca
     9841 cacccatagt aataaacata tccgtatctt ggacggccag gatattacca aaaaggatat
     9901 ggaaaaggtc agttgaactt ttagcgacac caatcaagat gataccgagg gctaagaaag
     9961 aagaaaaggt aatgccgatg gcggtatcgc ttttgataat cgagtttcct ttgatgtagg
    10021 taatgatgat ggcagctagc aatccaaaga caatggctcc gataaagaag tcaaggccca
    10081 agatgaagga gagggctaca cctggtaaga cagcatgtga aatggcatct cccatgagtg
    10141 acatcccgcg tagaataatg aaacatccca cggctccagc tacaatcccg acgacaatag
    10201 ctgttatcaa ggcattttgt aggaaatgga atttttgcaa tccatcgata aattctgcaa
    10261 tcataggtca cctccattga aaaagagttg attaccgtaa gcttctttta gattggtttc
    10321 ggtaaaagtt tcttttgttg gaccaaaggc aatcacttct cgattgacaa gtaagacttg
    10381 atcgaagtag tggggaatct tgctgaggtc gtggtgaacg atgagaaccg tcttcccagc
    10441 ttttttcaaa tctctcagcg tattcatgat gatttcctca ctgacagagt caatcccagc
    10501 aaagggttca tccaagagga tatagtcggc ttcctgcacc aaacatctgg caatcaagac
    10561 ccgctggaat tgacctccag acagttgact aatttgacgt tcagcgtagt cagctaggcc
    10621 gacgatttca agggcctctt gcactttctt ccaatgttta gcctttaaac ttcgaaagag
    10681 aggaatagag ggaaatagtc ctaacgagac gcattccttg accttgatgg gaaagttgta
    10741 gtcgatattg attttttgtt cgacataggc aattcggtgt aaggattttt taacttcctt
    10801 gtcatcgaga aatgcctgac cttgatgtgg gataattccc agcatacctt ttaatagtgt
    10861 tgatttccca gcgccgtttg gaccaatgat gccggtaatt gttggtccat ggagcactag
    10921 tgaaatatcc ttaagtgcca acgtttcttt ataggagaca ctgaggtttt cgatacgtat
    10981 cataaacttg tattcctcct gtctcttaat atacattaaa aaaaaaatta agtcaagtta
    11041 atttttgaaa aaattaaaat aataactgaa aaatagattc taaagataac tttcaggata
    11101 aatttctaaa ttataaaacg catagtatca agtgtaaaaa acttggaatt atgcgtttta
    11161 tcatggaaag gttttttata atagctaaaa aataatagtt aaatggttat taattgcatt
    11221 ccctagtgat ttttgttaag ataaatgcaa atacaaatga aagcgagaac aagatgacac
    11281 gttatcaaga tgatttttat gatgctatca atggagaatg gcaacagaca gctgaaatcc
    11341 cagcagataa gtctcaaaca ggaggttttg ttgatttaga ccaggaaatt gaagacctga
    11401 tgctggcgac aacagacaag tggttagcag gtgaagaagt gcctgaggat gctatcttgg
    11461 aaaactttgt caaataccac cgcctagttc gtgattttga caagagagaa gctgacggta
    11521 tcacacctgt cttaccactc cttaaagaat tccaagaact ggaaactttt gcggatttta
    11581 cagctaaact agcagagttt gagcttgcag gaaaaccaaa cttccttcct tttggtgtat
    11641 cgccagactt tatggatgct agaatcaatg ttctatgggc tagcgctcca agcacaatct
    11701 tgccagatac gacctactat gcagaagaac atcctcagcg cgaagagctc ttgactcttt
    11761 ggaaagaaag cagcgcaaat ctcctcaagg cttatgattt ctctgatgaa gaaattgaag
    11821 acttgctaga aaaaagactt gaattggacc gccgagttgc ggcagtggtg ctctctaatg
    11881 aagaaagttc agaatatgct aaactctatc atccatattc ttacgaagat ttcaagaaat
    11941 tcgcgcctgc cctacctttg gatgacttct tcaaagcagt tattgggcaa ttaccagaca
    12001 aggttattgt agacgaggaa cgtttctggc aagcagcaga gcaattctac agtgaggaag
    12061 cctggtctct ccttaaagca accttgattt tgagtgttgt caatctttca accagctatt
    12121 taacagagga tatccgtgtt ttgtctggtg cctacagccg tgccctttct ggagttccag
    12181 aggcaaaaga taaggtcaaa gcagcttatc atctagcaca agaacctttc aagcaagccc
    12241 tgggtctttg gtacgcccgt gagaagttct ctccagaagc caaggcggat gtggagaaaa
    12301 aagtggcaac catgattgat gtctataagg agcgtctgct taagaatgac tggctcactc
    12361 cagaaacctg taaacaggct atcgtgaagc tcaatgtgat caaaccttat attggctatc
    12421 cagaagaatt gcctgcacgt tacaaggata aggtagtgaa tgaaactgcc agtctttttg
    12481 agaatgctct agcctttgcg cgtgtggaaa tcaagcacag ttggagtaag tggaaccagc
    12541 ctgtagacta taaggaatgg ggcatgcctg ctcatatggt caatgcctac tacaatcctc
    12601 agaagaacct gattgtcttt ccagcggcca ttttacaggc gcctttctat gacttgcatc
    12661 agtcatcttc tgctaactac ggtggtattg gggcagtgat tgcccatgaa atttcccacg
    12721 cctttgatac taacggagct tcctttgacg aaaatggtag cctcaaggat tggtggacag
    12781 agagcgacta tgctgccttc aaggagaaaa cacaaaaagt cattgaccaa tttgatggac
    12841 aggattctta tggagcaacc attaacggta aattgactgt atcagaaaac gtggctgact
    12901 tgggaggaat cgcagcagcg cttgaagcag ctaagagaga agcagacttc tcagcagaag
    12961 agttcttcta caacttcggt cgcatctggc gcatgaaagg tcgatcagaa tttatgaaac
    13021 ttttggctag cgtcgatgtg cacgcaccag ccaaactccg tgtcaatgtg caagtaccaa
    13081 acttcgacga tttctttaca acatatgatg tcaaagaagg agacggaatg tggcgttcac
    13141 cagaggagcg cgtgattatt tggtaatgca aaaaatctag tcatcagaaa aaaggcatcc
    13201 aatcacgtgt gaaaaccttg ataggatgcc ttttataata gaaagatttg ctggatagtt
    13261 tacttaaatt tcgattgtca tcatcttgct aaaaaaaggg attgaaggcc ttttcgtgag
    13321 cgatagtagt agctggacca tgtcctggat agacatcgta gtttggtagg gtgaagagtt
    13381 gggtctggat actatgaagg agttgctcca tgctaccagt cggaaggtcg gtccgtccga
    13441 tagtttcgcg gaatagagca tctcccgtca agactaggtg agcatcagga aagactaggg
    13501 aaacaccacc gatagagtgc cctggggtcg gtagaacctt aaaacgaaat tcctctagtt
    13561 ggtattcttc gtgaaagaca aaggtgtgtt ctgcaggttt tgtgaccaca tctgccatat
    13621 catcgtggcg agggagaccg gagagattat cgacaggagt gtagagccag ctggcttcgc
    13681 tctctgcgat atagacagga ggattgccaa acgtctcgcg aaccaagtcc agactcatga
    13741 tatggtcata atgggcgtgg gtcaagagaa tagcacagat cggtttgttg atcttctcga
    13801 ttgtctgacg aatggcttcc caatgactac caggatcgac gacgatgagg tgcttttcgc
    13861 cttctagata ataggtattt tcataggcaa caggattcac ggttttatgg attttcatac
    13921 tagctccaat ctcaaagaat gtactaatac tcttcgaaaa tctcttcaaa ccgcgtcaac
    13981 tttatctgca acctcaaagc agtgctttga acagcctgcg gctagctttc tagtttgctc
    14041 tttgattttc attgagtata aattaagtat agcacagtta gggagaatag gtaaggattt
    14101 aaaatgaact taaaaaccag catgactaga tgaagacagg ctggttatca atttatcaat
    14161 attggaaagg tgtcaattgc ccttcatagg ttgcataaac tccgtcatta gcttgtttga
    14221 tagacgtgat gtttagagtg ctaccgtagt tggcttctca tttgtcgcta tattcactat
    14281 aagttatcct actgggcagg ttctggtcgt tagcatagta ttgaacgagt tttttatagc
    14341 tttgctggct gaataagaac aggcagcaaa ctcctaacat taggagagca aagatagaaa
    14401 tagcagtttt ttcataggta ttctccttat aaactaaagg atagaagaaa gtatcttgag
    14461 gatttttata ctcttcaaaa atctcttcaa accgcgtcaa cgtcgccttg ccgtatatat
    14521 gtgactgact ttgtcagtct tatctacaac ctcaaagcag tgctttgagc aacctgcggc
    14581 tagtttccta gtttgctctt tgattttcat tgagtattaa ttccaaaatc cagtcaaggt
    14641 accagtgtat gtgatacgag ttccaccgtt tatctttcgt atcttcaatg gaattgggga
    14701 aatcagaaag tctttgggtt acaatatggt ttccgttctt ctatctctcc ttctgtcacc
    14761 taagtttaaa atctaacttc gtagtatatt atgggccaat aattcagaat tatcaagcaa
    14821 ttttgaatta tttttttctt attctgttcc aatctagttt tcatattctt aaaaaagtga
    14881 taaaatggta ggaagaattg gagagtagag atgccgaaag aagtgaattt aacaggcgaa
    14941 gaagttgtcg ctttaaccaa agaatattta acggaagagg atgttcattt tgtccataag
    15001 gccttggtct atgctgttga atgccacagt ggtcaatatc gcaaatcagg cgagccttat
    15061 atcattcacc ctatccaagt ggcaggtatt ttagctaagc taaagctgga tgctgtaaca
    15121 gtagcttgtg gattcttgca tgatgtggtg gaagatacag atgcgacctt ggacgatttg
    15181 gaaagagagt ttggtcctga tgtgcggatg attgttgacg gagttaccaa gcttggcaag
    15241 gtcgagtaca aatcgatcga ggagcaatta gcggaaaatc atcgcaagat gctcatggcc
    15301 atgtctgagg acatccgcgt tattttggtc aaactgtctg accgcttgca caatatgcgg
    15361 actctgaaac atcttcgaaa agacaagcag gagcgtattt ccaaagaaac catggaaatc
    15421 tatgccccac ttgcccatcg tttggggatt tccagtgtca aatgggaatt agaagacttg
    15481 tctttccgtt atctcaatcc aacggagttt tacaagatta cccatatgat gaaggaaaag
    15541 cgcagggagc gtgaggcctt ggtggatgag gtagtcacaa aattagagga gtatacgaca
    15601 gaacgtcact tgaaagggaa gatttatggt cgtcccaagc atatttactc aattttccgc
    15661 aaaatgcagg acaagagaaa acggtttgag gaaatctatg atctgattgc tattcgttgt
    15721 attttagata cccaaagtga tgtttatgcc atgcttggtt acgtgcatga attttggaaa
    15781 ccgatgccag gtcgcttcaa agactatatt gccaaccgca aggccaatgg ttatcagtct
    15841 atccatacga ctgtttatgg accaaaaggg ccgattgaat tccagattcg aaccaaggaa
    15901 atgcacgagg tggctgagta cggggttgcg gctcactggg cttataagaa aggtataaag
    15961 gggcaagtta acagcaagga atcagctatt ggaatgaact ggatcaagga gatgatggag
    16021 ctccaagacc aggctgatga tgctaaggaa tttgtggact ctgttaagga aaactatctg
    16081 gctgaggaga tttacgtttt taccccagat ggagctgtcc gttctcttcc caaagattca
    16141 ggaccgattg attttgccta cgaaatccat accaaggtcg gtgaaaaagc aactggtgcc
    16201 aaggtcaatg gccgcatggt tccactgaca accaagttaa agacagggga tcaggttgaa
    16261 attatcgcca acccgaactc ctttggacct agccgtgact ggctcaatat ggtcaagact
    16321 agcaaggcgc gcaataagat tcgccagttc tttaaaaacc aagataagga attgtctgtc
    16381 aacaagggtc gtgagatgct gatggctcag ttccaagaaa atggctatgt ggcaaataaa
    16441 tttatggaca agcgccacat ggatcaagtt ctgcaaaaga ccagttacaa gacagaagac
    16501 tccctctttg cggccattgg ttttggggaa atcggtgcga ttaccgtctt taaccgtctg
    16561 actgaaaagg aacgccgtga ggaagagcgt gccaaggcca aggctgaggc agaggagctt
    16621 gtcaaaggtg gcgaggtcaa ggttgaaaat aaagaaactc tcaaggtcaa gcatgagggg
    16681 ggagtggtta ttgaaggtgc ttctggtctc ctagtgcgga ttgctaagtg ttgtaacccc
    16741 gtgcctggtg acgatattgt tggctacatt accaagggtc gtggtgtggc tattcaccgt
    16801 gtggactgta tgaacctgcg tgcccaagaa aactacgagc aacgtctcct tgatgtggaa
    16861 tgggaagacc agtactctag ctcaaataag gagtatatgg cccatatcga tatctacggt
    16921 ctcaaccgta caggactgtt gaacgatgta ctgcaagttc tttcaaatac aaccaagaat
    16981 atttcaacgg tcaatgccca accaaccaag gatatgaagt ttgctaatat ccatgtgtcc
    17041 ttcggtattg ccaacctctc tacactgacc acggttgtcg ataaaattaa gagtgtgcca
    17101 gaagtttact ctgtcaaacg gaccaacggc tagttgtcct agctcttact agaaaggcta
    17161 ttatgaaaat cattatccaa cgggttaaaa aagcccaagt gagtatagaa ggccagattc
    17221 agggaaaaat caatcaggga cttttattgc tggttggtgt tggaccagag gaccaagagg
    17281 aagatttgga ctatgctgtg agaaaactgg tcaatatgcg gattttttca gacgcagaag
    17341 gcaagatgaa cctgtctgtc aaagatattg aaggagaaat cctctctatt tctcagttta
    17401 ccctctttgc ggatactaag agaggcaatc gtccagcctt tacaggggca gctaaacctg
    17461 atatggcatc agacttctat gatgctttca atcaaaaatt agcgcaagaa gtgcccgttc
    17521 agacaggcat ctttggagca gatatgcagg ttgagctggt taataatgga cctgttacca
    17581 ttatcctaga tactaaaaag agataagaaa gaccaagccc agtcggcttg gtctttctca
    17641 tctatcataa aatactccaa aaagaaatcg gttcttgata tgcttggggg aagtctcttt
    17701 tcaggctttg gcagatgcga taggaaggga tgagatgtcc taggatgagg agagttccct
    17761 gaaggaaaag tggaatatca cttaaagaca ccaaggagag aatcatcagg ctatcccata
    17821 ggagaatttg taaagcaaaa cgccagaatc tcggtctaat ctgggaatag agttaactaa
    17881 agtggtcaca aagttggttt gaaagatagg tcaatagcac aggatgaaag aaaatgagcc
    17941 aaccgctata aatcaaaatc cagtcccaag taagccccct tttaaatgtc aaaaatgcca
    18001 gcatgattcc atcaatgaca aggagttgaa tggttttgat gaagatgatt tttttcatgc
    18061 taaaacctcc ttttctttag agacactccc ataaagagat gtgtttgtcg taaaagtctg
    18121 gatagttttc tcttaggtag tcagctgtca tataataaaa agtagaatgg caagcagtga
    18181 tgattggcat aagagatgaa aaacgctgag aactaatgaa ggctaagaga acaaaaaata
    18241 gaaaatcaat ggtgagaacc cctaaatagt agaagcgaat ctttttattg atttgaggat
    18301 aaatctcagc gaattgatgt ttgagtcgga cgaccaagag aaatagcagg agtccttgag
    18361 caaggaagaa aattaaacca gagagcaaga gccactcaaa agatgtctcg agtctagtga
    18421 taaaaaatgc aaatagtagc aaatcaataa ctgcaattgc tataaaatgg attctataga
    18481 gttttttcat gacaagacct ccctctttta tctaacttca ttctactcca aaagaatggg
    18541 agttacaact aaaatgataa aaatagcaga agggagattc tcttaagttg gctagtattc
    18601 tttatttgag tttccttcta ttatctaact tcttcatcat tccagacaaa taaagctccg
    18661 attgcattga ggatataaaa gatgtattta ccgatattgg cgaagtttcc ttgaatacca
    18721 gcttttgtca gctgaacgaa attgtaaatc aaccaaaagc cccactgagt tgttagtttt
    18781 aatgcattca aagcattggc aatgagggac agtgcaaagg caatagttgt tacgtaggca
    18841 aggagattca tcttgccccc atatccgata tagttggtca caaaggcaaa gaggaaggcg
    18901 atgatggaaa tgatgatggc cgccaatttt acctgttttt ggctcatttg gttgggtctg
    18961 ccttcttgcg aagcttccca cttctttata gcaaaggtat aaatgaggaa ggtgacggga
    19021 taggtaatga tggccgcctt atttccaagg atataatcaa tagcaccgga caaaatggta
    19081 ttaacaatac caaagtaatt tccccatttg cttaatttcc ccgtgaaacg agtggacaac
    19141 atggaaatcc caacgttggt tacggaaatc aatccaaagg gtacaagagc tgtccatgat
    19201 ccccagtcta caaatttatc gaggtgtgag ttgaggtaac cagatgcaat cgcaatccca
    19261 acgaccaaag caaccccgaa gaggtcaaac tatttagatg tagcaaaaat ttttagtgat
    19321 tttttcatag gttaaactac ctttcttttt ttcaaatatt ctcccaccaa atgaaagtaa
    19381 aataaaatga tagaaataaa accctgaaaa ta
//
LOCUS       DAVHEX010000048        18868 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVHEX010000048 DAVHEX010000000
VERSION     DAVHEX010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18868
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.048"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            127..282
                     /locus_tag="VVL45_001582"
                     /pseudo
     CDS             127..282
                     /locus_tag="VVL45_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
     gene            482..3757
                     /gene="rexB"
                     /locus_tag="VVL45_001583"
     CDS             482..3757
                     /gene="rexB"
                     /locus_tag="VVL45_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEU4187738.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLEYLSQQASFSITVTRFAQMARYLVLNDLPAKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQESQLSHLIEAIENDKVSSDFNQIALVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQTFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAEKLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQATEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDLQGLENPAIPTSPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWFEKIREE
                     LNR"
     gene            3754..7404
                     /gene="addA"
                     /locus_tag="VVL45_001584"
     CDS             3754..7404
                     /gene="addA"
                     /locus_tag="VVL45_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEU4187739.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMVERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKESFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQEFDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FTKLGQLSDQIAILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDINHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNMHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRNRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKAHENLYEKLVNAQKKAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKPHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAIELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLESKEYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTVLGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            7664..8842
                     /locus_tag="VVL45_001585"
     CDS             7664..8842
                     /locus_tag="VVL45_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187740.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSFIFNGIGRSNC
                     VCGNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSNFDERLLKWFWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWILNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
     gene            9051..15065
                     /locus_tag="VVL45_001586"
     CDS             9051..15065
                     /locus_tag="VVL45_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU4187741.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGIGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSESEVSNQKSSVATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYREETIETKIDFQEEI
                     QENPDLAEGTVRVKQEGKLGKKVEIVRIFSVNKEEVSREIVSTSTTAPSPRIVEKGTK
                     KTQVIKEQPETGVEHKDVQSGAIVEPAIQPELPEAVVSDKGEPEVQPTLPEAVVTDKG
                     ETEVQPESPDTVVSDKGEPEQVAPLPEYKGNIEQVKPETPVEKTKEQGPEKTEEVPVK
                     PTEETPVNPNEGTTEGTSIQEAENPVQPAEESTTNSEKVSPDTSSKNTGEVSSNPSDS
                     TTSVGESNKPEHNDSKNENSEKTVEEVPVNPNEGTVEGTSNQETEKPVQPAEETQTNS
                     GKIANENTGEVSNKPSDSKPPVEESNQPEKNGTATKPENSGNTTSENGQTEPEPSNGN
                     STEDVSTESNTSNSNGNEEIKQENELDPDKKVEEPEKTLELRNVSDLELYSLSNGTYK
                     QHISLEQVPSNPNSYFVKVKSSSFKDVYLPVASISEERKNDKILYKITAKVEKLQQEI
                     ESRYKDNFTFYLAKKGTEETTNFTSFSNLVKAINQNPSGTYHLAASLNANEVELGPDE
                     RSYIKDTFTGRLIGEKDGKNYAIYNLKKPLFENLSGATVEKLSLKNVAISGKDDIGSL
                     ANEAQNNTKIKQVHVDGVLAGERGIGGLLAKAEQSSITESSFKGRIINTYETTAAYNI
                     GGMVGHLTGDKALLTKSKATVAISSNTNTSDQTVGGLAGLVDRDAQIQDSYAEGDINN
                     VKHFGRVAGVAGNLWDRTSGDVRHAGSLTNVLSDVNVTNGNAITGYHYNEMKVKDTFS
                     SKANRVYNVTLVKDEVVSKESFEERGTMLDASQIASKKAEINPLILPTVEPLSTSGKK
                     DSDFSKVAYYQAKRNLTYKNIEKLLPFYNKATIVKYGNLVNENSLLYQKELLSAVMMK
                     DNQVITDIVSNKQTANKLLLHYKDDLSEKLDLKYQNDFAKLAEYSLGNTGLLYTPNQF
                     LYDQTSIIKQVLPDLQKVDYHSEAIRKTLGISPNVKQTELYLEDQFAKTKQQLEDSLK
                     KLLSADAGLASANPVTEGYLVDKIKRNKEALLLGLTYLERWYNFSYGQVNVKDLVLYH
                     LDFFGKGNASPLDTLIELGKSGFNNLLAKNNVDTYGISLASQHGTTDLFSTLEHYRKV
                     FLPNTSNNDWFKSETKAYIVEEKSTIEEVKTKQGLAGTKYSIGVYDRITSATWKYRNM
                     VLPLLTLPERSVFVISTMSSLGFGAYDRYRSSDHKAGKALNDFVEENARETAKRQRDH
                     YDYWYRILDDNAREKLYRNILLYDAYKFGDDNTVGKATEVADFDNPNPAMQHFFGPVG
                     NKVGHNQHGAYATGDAVYYMGYRMLDKDGAITYTHEMTHDSDQDIYLGGYGRRSGLGP
                     EFFAKGLLQAPDHPDDATITINSILKHSKSDSTESRRLQVLDPTTRFNNADDLKQYVH
                     NMFDVVYMLEYLEGNSILKLDTNQKQQLLRKVTNEYHPDPDGNKVYATNVVRNLTVEE
                     VERLRSFNDLIDNNILSSREYASGKYERNGYFTIKLFAPIYAALSNDIGTPGDLMGRR
                     IAYELLAAKGFKDGMVPYISNQYEEEAKQKGKTINLYGKTRGLVTDDLVLEKVFNNQY
                     HTWSEFKKAMYQERQDQFDRLNKVTFNDTTQPWQTFAKKTTSSVDELQKLMDVAVRKD
                     AEHNYYHWNNYNPDIDSEVHKLKRAIFKAYLDQTNDFRSSIFENKK"
     gene            15117..15293
                     /locus_tag="VVL45_001587"
     CDS             15117..15293
                     /locus_tag="VVL45_001587"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU4187742.1"
                     /translation="MQAGIPTDNEVSYDLIYQQETLPATGSSTSELTALGLLAVGSLV
                     LLVHNMTGTVFCSL"
     gene            15512..16363
                     /gene="ylqF"
                     /locus_tag="VVL45_001588"
     CDS             15512..16363
                     /gene="ylqF"
                     /locus_tag="VVL45_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEU4187743.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            16350..17129
                     /locus_tag="VVL45_001589"
     CDS             16350..17129
                     /locus_tag="VVL45_001589"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEU4187744.1"
                     /translation="MATIKEIKEFLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGRGPLAGPVVAAAVILSKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELLKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            17145..18695
                     /locus_tag="VVL45_001590"
     CDS             17145..18695
                     /locus_tag="VVL45_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEU4187745.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
BASE COUNT         6192 a         3678 c         3894 g         5104 t
ORIGIN      
        1 tatagtagat tgaaataaga tacgaatagc ttgattaggg aaatcaaatt aatttctaga
       61 aatattttag catccacggt gtactattat agtttcaatt tactatagtt caatcattct
      121 atcaagaata ttgaatatat aaaggcacgt aatattaaaa cttcttctga tatcgaatta
      181 gagtccgatg ttgatggcga taaatcagac aatctaccag tcgacattaa gatactggga
      241 caacacattg aagtgtattc cgtacctaag aaacaatcat aaaactcaaa gccttgttac
      301 cgaggctttt ttattatgtg attttcagta gccagactta ttctatctaa tacaagtaaa
      361 gctaagtatt taagatttag cagatgatga agaaaagaaa ggatggccta aatattttaa
      421 cagatttacg aggctaatca tggtataata tagggtgatg aaaaattcca acgaggctga
      481 gatgaaatta ctttatactg atattcggac ttctttgaca gaaattctaa caagagaggc
      541 agaagagcta gttgcagctg gcaagcgggt cttctacatt gcccccaact ctctttcttt
      601 tgaaaaggaa cgcgccgtgc tggaatactt gtcccagcag gcttcttttt cgattaccgt
      661 cacgcgcttt gctcaaatgg ctcgctatct ggtcttgaat gatttaccag ctaaaactac
      721 tcttgatgat atcggtcttg ggttggcctt ttacaaatgc cttgccgaac tcgatcccaa
      781 ggacttgcgt gtttatggcg ctattaagca ggatcctcaa ttgatccagc agttaattga
      841 gctttaccat gagatgacca aatctcagat gagttttttg gacttggaga atttaacaga
      901 tgaggataag agggcggatt tactcttgat ttttgagaaa gtaacagcct atcttaatca
      961 aggtcagtta gcccaggaaa gtcagttgtc ccatttgatt gaggctattg agaatgacaa
     1021 ggtaagtagt gattttaatc aaatcgcctt ggtcattgac ggctttactc gtttttctgc
     1081 tgaggaagag cgggttgtgg acttacttca cggcaaaggt gttgagattg ttatcggggc
     1141 ttatgctagt aagaaagcct ataccagtcc ttttagcgag ggcaatctct accaagccag
     1201 cgtaaaattt ctccatcatc tggcttctaa ataccaaacg cctgctcagg actgttctca
     1261 aactcatgag aagatggata gttttgacaa ggcctctcgt ttgttggagt cttcttatga
     1321 cttttcagaa ctcgctttgg atgtcgatga gaaagaccgt gaaaatttac aaatctggtc
     1381 ttgtttgacg caaaaggagg agttggagct agtagcccgt agtattcgtc agaaattaca
     1441 tgagaactca gacctgagct acaagcattt tcgtattctc ttgggggatg tagcttctta
     1501 ccagttatct ctcaaaacca tttttgacca gtatcagatt cctttttatc ttggtagaag
     1561 cgaagccatg gctcatcatc ccttgactca gtttgtcgag tctattttag ctttaaaacg
     1621 ttaccgtttt cgtcaggagg atttgattaa tcttcttaga actgatttgt atactgacct
     1681 cagtcagtct gatattgatg cttttgagca atatatccgc tatcttggta tcaatggctt
     1741 gccagccttt cagcaaacct tcaccaaatc ccaccatgga aaatttaatc ttgagcgttt
     1801 gaatgtcctc cgcctgagaa ttttagcacc tcttgaaacc ctctttgcca gccgaaaaca
     1861 aaaggctgaa aaactcctac aaaaatggag tgtctttcta aaagaaggag ctgtgaccaa
     1921 gcagttacaa gatttgacaa ccactttgga agctgtagaa caggaaagac aagccgaagt
     1981 ttggaaggct ttctgccatg ttttagaaca atttgcgact gtttttgctg gttcacaggt
     2041 tagtctggaa gacttcctag ccttgctcca ttctggaatg agtttgtccc aataccgtac
     2101 cattccagca acagtggaca ctgttctggt gcagagttac gatttgattg caccattgac
     2161 tgctgacttt gtctatgcta ttggactaac tcaggacaat ttaccaaaaa tttctcaaaa
     2221 caccagtctt ctgacagatg aagaaaggca aaatctaaac caagcgaccg aagaaggcgt
     2281 tcaattactg attgccagca gtgaaaatct caagaaaaat cgctacacta tgctttcctt
     2341 ggtcaattct gctcgtaagc agttgttctt gtcggctcca agccttttta acgaaagtga
     2401 aagtaaggaa tctgcctatc ttcaagagtt gatccatttt ggatttaggc ggagagagaa
     2461 gaggatgaat cacaaaggac tgtctaagga ggatatgggg tcctatcaca gtcttttgtc
     2521 tagtctggtt gcctatcacc agcagggtga gatgagcgat actgagcaag atttgacttt
     2581 tgtcaaggtt ctgtcgcgtg tcataggtaa aaaactagat ctgcaaggtc tggaaaatcc
     2641 agctatccca accagtccaa gcagcaagac cttagccaag gacaccttgc aagctctcta
     2701 tcctgccaaa caggagtttt acctgtctac gtcgggtttg acagagtttt atcgcaatga
     2761 atacagttat ttcctacgct acgttttagg cttgcaggag gaattacgtt tgcgtcctga
     2821 tgcccgtagt cacgggaatt tcttgcatcg tatctttgaa cgcgccttac agttgcctaa
     2881 tgaagattcc tttgaccaac gtctagaaca agctattcaa gaaaccagtc aagaacgcga
     2941 atttgaagct atttatcaag aaagtttgga agcccagttt accaaggaag ttttgcttga
     3001 tgttgcacgg acgactggac atattctccg acacaatcca gccatcgaaa ccatcaaaga
     3061 agaagcaaat tttggtggaa aagaccaagc ctttattcaa ttagacaatg gacgcagtgt
     3121 ctttgtacga ggcaaggtgg accggattga ccgtttgaaa gctaatggag cgataggagt
     3181 agtagactac aaatccagtc tgactcagtt ccagtttcct catttcttta atgggctcaa
     3241 ttctcagtta ccaacctatc ttgctgccct aaaaagagaa ggggagcaga actttttcgg
     3301 cgccatgtac ttggaaatgg ctgaacctgt ccaatctctg atggcggtaa aaagtctggc
     3361 aggagcagtg gtagaagcca gcaaatctat gaaataccaa gggctcttct tggaaaaaga
     3421 aagcagttat ttaggcgaat tttataacaa aaacaaggct aatcaactga cagatgagga
     3481 atttcagctc ctactggact acaatgccta tctttacaag aaagctgctg agaagatttt
     3541 agcaggccgg ttcgccatca atccttatac tgaaaatggc agaagcattg ccccatacgt
     3601 ccagcaacat caggctatta caggctttga agccaattac catctgggcc aagcccgttt
     3661 cctagaaaag ttggacctag ctgatggcaa gcgtctggtc ggagaaaaac tcaagcaagc
     3721 ttggtttgaa aaaataagag aggagttaaa tcgatgaagc ttattccctt tttaagtgag
     3781 gaggagattc aaaaactgca agaagcagaa gcaaattcga gcaaggaaca gaagaaaact
     3841 gccgaacaaa tcgaagctat ctacacttct ggtcagaata tcctggtctc agcatcggct
     3901 ggttctggaa agacctttgt catggtagag cgcattctgg accaattggc gcgtggtgtc
     3961 gaaatttctc aactctttat ctcaaccttt accgtcaagg ctgcaactga acttaaagaa
     4021 cgtttagaga aaaaaatcag caagaaaatc caagaaacag atgatgtcga tctcaaacaa
     4081 cacttgggtc gccagttggc agacctaccc aacgctgcca tcggaaccat ggactctttc
     4141 acacaaaaat tccttggcaa acatggttat ctgcttgata ttgcacctaa tttccgtatt
     4201 ttacaaaacc aaagcgagca acttcttctc gaaaacgaag tctttcatga ggtctttgaa
     4261 gcgcattacc aaggtaaaca gaaagagagc tttagtcatt tgctgaaaaa ctttgctggg
     4321 cgtggcaagg acgaacgggg tctgcggcag caagtctata aaatctatga cttcctccaa
     4381 tccaccagta atcctcaaaa gtggctgagt gaatctttcc tcaaaggctt tgagaaagct
     4441 gattttacca gtgaaaaaga aaaactgact gagcaaatca aacaagccct ctgggatttg
     4501 gaaagctttt tccgttacca tctggataac gatgccaagg agtttgcaaa ggctgcctat
     4561 ttagaaaatg ttcagttaat tctggatgaa attggctccc taaatcagga gttcgatagt
     4621 caggcttatc aggcagtact tgcgcgtgtt gtcgccatct ctaaggagaa aaacggtcga
     4681 gctctgacta atgccagccg taaggctgat ttgaagcccc tggctgatgc ctacaacgaa
     4741 gagagaaaga cccagtttac taaactagga caattatcag accagatagc gattctcgac
     4801 tatcaagaac gttatcatgg agacacttgg aaactagcta aaaccttcca atctttcatg
     4861 agcgattttg tagaggctta tcgtcagaga aaacgacagg aaaatgcctt tgaattcgct
     4921 gatatcagcc attacaccat tgagatttta gaaaatttcc cacaagttcg tgagtcttat
     4981 caggagcgct tccatgaagt catggtcgat gagtatcagg atatcaacca tattcaagaa
     5041 cggatgctgg aattgttgtc taatggccac aatcgcttta tggtgggaga tatcaagcaa
     5101 tccatctatc gtttccgtca ggcagacccg cagattttca atgagaaatt ccaacgctat
     5161 gcgcaaaatc ctcaagaagg caagctcatt ctcctcaagg aaaatttccg tagtagttca
     5221 gaagtgctgt cagcaaccaa tgatgtcttt gaacgtctca tggaccaaga ggtcggcgaa
     5281 atcaactatg ataacatgca ccagcttgtt tttgccaata ccaaactgac tcccaatcca
     5341 gacaacaagg ccgaatttct cctctacgac aaggacgata caggtgagga agaagagagt
     5401 caaacagaaa cgaaactaac aggcgaaatg cgcttagtta tcaaggagat ccttaaactt
     5461 catcaggaaa aaggtgttgc ctttaaggaa attgcccttc tgacctctag tcgtaatcgt
     5521 aatgaccaaa ttctacttgc cctgtctgag tacgggattc ctgtcaaaac tgacggagag
     5581 caaaacaatt atctccaatc cctagaagtg caagtcatgc tagacaccct tcgtgtcatt
     5641 cacaatcccc tgcaagacta cgccttggtt gcccttatga agtctccaat gtttggtttt
     5701 gatgaggatg agctagcacg tttgtccctt cagaaagcag aggataaagc ccacgaaaat
     5761 ctctatgaga aactagtcaa tgcacaaaaa aaggcaagta gtcaaaaagg cttgattcac
     5821 acagctctag ctgaaaaact aaagcaattc atggatatcc tagcttcttg gcgcttgtat
     5881 gccaaacccc actctctcta tgacttgatt tggaagattt acaacgaccg tttttattat
     5941 gactatgttg gggctttgcc gaatggtcct gctaggcagg ccaatctcta tgccctagca
     6001 ctgcgtgctg atcaatttga aaagagcaat ttcaaaggtt tgtcgcgttt tattcgtatg
     6061 attgaccaag tcttagaagc ccagcacgat ttggcaagcg tggccgtcgc accgccaaaa
     6121 gatgcaatag agctcatgac catccacaag agtaaagggc tggagtttcc ttacgtcttt
     6181 atcctcaata tggatcaaga tttcaacaaa caagactcta tgtcagaagt cattctcagt
     6241 cgtcagaatg gtcttggtgt caaatatatt gccaagatgg agacaggggc agtagaagac
     6301 cactatccta aaaccatcaa actctccatt cctagtctga cctataggca gaacgaagag
     6361 gaattacagc tagcaagcta ttctgagcag atgcgtttgc tgtatgttgc tatgacgcgg
     6421 gctgagaaaa agctctatct tgtcggcaag ggttctcgtg aaaagctgga atccaaggaa
     6481 tacccagcag ccaaaaatgg gaaactaaat agcaatacta gactgcaagc acggaatttc
     6541 caagattggc tttgggctat cagtaaagtg tttactaagg acaagctcaa ctttagttat
     6601 cgttttattg gcgaagatca gttgaccaga gaagctatcg gagagttgga aaccaagagt
     6661 cctctccaag atagctccca agcagacaat cgtcagtcag ataccatcaa agaagctctg
     6721 gaaatgctga aggaggtgga agtttataat actcttcacc gcgcagctat tgaacttcct
     6781 agtgttcaaa ccccaagtca aatcaagaaa ttctacgaac cagttatgga tatggaaggt
     6841 gtcgagattg ctggtcaagg tcagtcagta ggcaagaaaa tcagcttcga tttgccagat
     6901 ttttcaacca aagaaaaggt aactggagct gagattggta gtgctactca cgaactcatg
     6961 cagagaattg acctcagcca gcaactaacc cttgctagcc taacagaaac actcaaacaa
     7021 gttcaaacta gccaagctgt cagagacaag atcaatcttg ataaaattct tgctttcttt
     7081 gacacagtac tcggtcagga aattcttgct aataccgacc atctttatcg cgagcaacct
     7141 ttctccatgc tcaaacgaga ccaaaagagt caggaagact ttgttgtccg tggtatcctt
     7201 gatggctatc tgctttacga aaacaaaatt gttctgttcg actacaagac agaccgctat
     7261 gatgaaccaa gtcaactcgt agaccgctat cgtggtcagt tagctctata cgaagaggct
     7321 ttatcacgag cctattcgat tgaaaatatt gaaaaatact tgattttact cggtaaagac
     7381 gaggttcaag ttgtaaaagt ataacttata ctctttgaaa atctcttcaa accacgtcag
     7441 cttcgccttg ctgtatatat ggttactgac ttcgtcagtt ctatccacaa cctcaaaaca
     7501 gtgttttgag ctgacttcgt cagttctatc tacaacctca aaacagtgtt ttgagctgac
     7561 ttcgtcagtt ctatctacaa cctcaaaaca gtgttttgag ctgattgcgg ctagctttct
     7621 agtttgctct ttgattttta ttgagtatta gaaaggagac ctcatgacac ttccagttag
     7681 aaaatccctg cacgatgcag ttttacaggc ttcgaaagcg gatacttggg aacaagctac
     7741 caaggaatgg aatgaagttt cctttatttt taacggtatc ggccgtagca attgtgtctg
     7801 tggaaatgcc atcaaatacg cttatgaact ctttaacggt gtcacaggcc aacgcctctt
     7861 tccaattggt agcgactgtg ttcgtcattt tcatcgattg tcccttgacc agcaattgga
     7921 agaggaagaa aaactgctca gaaaggttga aaatctaacc agaaaagctc agaaaaagga
     7981 aaaaatcaag gtcaataaaa gtaactttga cgagcgactt ctaaaatggt tctgggaaaa
     8041 aggtgttttc aaacccaatc gtggcaatca atttgcgcct gagagagact accagctttt
     8101 cctagaagtc tttcagggag gaagttggac caaggcggaa ccaaagaaaa aggctcggat
     8161 ggaagaagtc cttgaaaagt gtatcaaacc ctttttactt gggaaatcgg atgaccaact
     8221 ctaccttgtc aagctaggca aggagaaaat tgactacgaa caagaactcc gtatccaggc
     8281 agagaaagaa cgcaagaaga gggataaaat cgccaagcaa tatgcagaca atctggttct
     8341 agccatggga cccgcagagc gtgcttatca agattacttt ggctttacag agaccctgac
     8401 tcaagaagaa cgcaagtggg aaaaaattct ttttggtaaa aatagagcag aacgagctat
     8461 caaggccaaa caataccaaa aagagctgga aaaagaccaa cgaattgcca gtcaggatcc
     8521 aattgaaaga aaacagaagc agacttggat cctcaattcc tattttcgtg agcttcctga
     8581 agaaaaagct cgattttcta gactcttatt agaatatcga aaaagtggtg aagtaccctt
     8641 ttcaactgaa tacctgtcag atcatctcat cgactttttc tataaaatga aagcctttga
     8701 atttgaaatc gcaccagaac aagtccgaga ttttctaaaa aaaagcctgc aggaagacca
     8761 tcgctcatca gcacaaggaa gctggataga aggaattctc ctcaactgcc tcaaaccatt
     8821 tttagaacga ttagtgatat gagtaacacc taccgtgtaa atttcatggt aggtgttatt
     8881 gtacccccaa ataatataaa acaaagtatg aaagcgcatc tcaattttaa gacttttttg
     8941 taaattaaga gtataattaa ggtatactgc cttttctata gataatagat aatacactaa
     9001 tataataaag gtctttgtaa catatataaa ttttatttca aggaggaata atggaaaagt
     9061 attttggtga aaaacaagag cgtttttcat ttagaaaatt atcagtagga cttgtatctg
     9121 caacgatttc aagtttattt tttatgtctg tattagctag ttcatctgtg gatgctcaag
     9181 aaactgcggg agttcactat aaatatgtgg cagattcaga gctatcatca gaagaaaaga
     9241 agcagcttgt ctatgatatt ccgacatacg tggagaatga tgatgaaact tattatcttg
     9301 tttataagtt aaattctcaa aatcaactgg cggaattacc aaatactgga agcaagaatg
     9361 agaggcaagc cctagttgct ggtgctagct tagctgctct gggaatttta atttttgctg
     9421 tttccaagaa aaaggttaag aataaaacgg tattacattt agtattggtt gcagggatag
     9481 gaaatggtgt cttagtttca gtccatgctt tagaaaatca tcttttgcta aattacaata
     9541 cggactatga attgacctct ggagaaaaat tacctcttcc taaagagatt tcaggttaca
     9601 cttatattgg atatatcaaa gagggaaaaa cgacttctga gtctgaagta agtaatcaaa
     9661 agagttcagt tgccactcct acaaaacaac aaaaggtgga ttataatgtt acaccgaatt
     9721 ttgtagacca tccatcaaca gtacaagcta ttcaggaaca aacacctgtt tcttcaacta
     9781 agccgacaga agttcaagta gttgaaaaac ctttctctac tgaattaatc aatccaagaa
     9841 aagaagagaa acaatcttca gattctcaag aacaattagc cgaacataag aatctagaaa
     9901 cgaagaaaga ggagaagatt tctccaaaag aaaagactgg ggtaaataca ttaaatccac
     9961 aggatgaagt tttatcaggt caattgaaca aacctgaact cttatatcgt gaggaaacta
    10021 tagagacaaa aatagatttt caagaagaaa ttcaagaaaa tcctgattta gctgaaggaa
    10081 ctgtaagagt aaaacaagaa ggtaaattag gtaagaaagt tgaaatcgtc agaatattct
    10141 ctgtaaacaa ggaagaagtt tcgcgagaaa ttgtttcaac ttcaacgact gcgcctagtc
    10201 caagaatagt cgaaaaaggt actaaaaaaa ctcaagttat aaaggaacaa cctgagactg
    10261 gtgtagaaca taaggacgta cagtctggag ctattgttga acccgcaatt cagcctgagt
    10321 tgcccgaagc tgtagtaagt gacaaaggcg aaccagaagt tcaacctaca ttacccgaag
    10381 cagttgtgac cgacaaaggt gagactgagg ttcaaccaga gtcgccagat actgtggtaa
    10441 gtgataaagg tgaaccagag caggtagcac cgcttccaga atataagggt aatattgagc
    10501 aagtaaaacc tgaaactccg gttgagaaga ccaaagaaca aggtccagaa aaaactgaag
    10561 aagttccagt aaaaccaaca gaagaaacac cagtaaatcc aaatgaaggt actacagaag
    10621 gaacctcaat tcaagaagca gaaaatccag ttcaacctgc agaagaatca acaacgaatt
    10681 cagagaaagt atcaccagat acatctagca aaaatactgg ggaagtgtcc agtaatccta
    10741 gtgattcgac aacctcagtt ggagaatcaa ataaaccaga acataatgac tctaaaaatg
    10801 aaaattcaga aaaaactgta gaagaagttc cagtaaatcc aaatgaaggc acagtagaag
    10861 gtacctcaaa tcaagaaaca gaaaaaccag ttcaacctgc agaagaaaca caaacaaact
    10921 ctgggaaaat agctaacgaa aatactggag aagtatccaa taaacctagt gattcaaaac
    10981 caccagttga agaatcaaat caaccagaaa aaaacggaac tgcaacaaaa ccagaaaatt
    11041 caggtaatac aacatcagag aatggacaaa cagaaccaga accatcaaac ggaaattcaa
    11101 ctgaggatgt ttcaaccgaa tcaaacacat ccaattcaaa tggaaacgaa gaaattaaac
    11161 aagaaaatga actagaccct gataaaaagg tagaagaacc agagaaaaca cttgaattaa
    11221 gaaatgtttc cgacctagag ttatacagtt tgtcaaatgg tacttataaa caacacattt
    11281 cgttagagca agttccaagc aatccaaata gctactttgt taaagtgaaa tcttcttcat
    11341 tcaaagatgt atacctacca gtagcatcaa tatcagagga aagaaaaaat gataaaatcc
    11401 tttataaaat cacagcaaaa gtagagaagc ttcagcagga gatagaaagc agatataaag
    11461 ataattttac cttctatcta gctaagaagg gaacagaaga aacaacaaac tttacttcct
    11521 ttagtaatct ggtcaaagct ataaaccaaa atccctctgg aacctatcat ttagcggcca
    11581 gcctgaatgc taacgaagtg gagcttggtc ctgatgaaag atcctatatc aaggacacct
    11641 ttactggtcg tttaatcggt gaaaaagatg gcaagaatta tgctatctat aatttgaaaa
    11701 aacctctgtt tgaaaacttg agtggtgcta cagtagaaaa actgagtcta aaaaatgttg
    11761 ctatttcagg gaaagatgat atcggttcac tggcaaatga agctcagaat aacacaaaaa
    11821 ttaagcaagt tcacgtcgat ggtgttctgg ctggtgaacg tggtatcggt ggtttgctgg
    11881 ctaaggctga gcaatcaagc atcacagaga gcagtttcaa gggaagaatt atcaacactt
    11941 atgaaacgac tgctgcctac aatatcggtg gtatggtcgg tcatttgaca ggtgacaagg
    12001 ctttacttac taagtcaaaa gcgacagtag ccatttcatc taacacaaat acttcagatc
    12061 agactgtggg tggacttgca ggcctagtag accgagatgc acagatccaa gatagctatg
    12121 ctgaaggtga tatcaacaat gtcaagcact ttggtagagt cgctggagtg gcaggcaatt
    12181 tgtgggatcg aacttctggt gatgttaggc atgctggaag tttgaccaat gttctcagcg
    12241 atgttaatgt aaccaacgga aatgccatca ctggttacca ctataacgaa atgaaggtaa
    12301 aggacacatt cagcagcaag gccaacagag tctacaatgt caccttggtc aaggatgagg
    12361 tcgtcagcaa ggaatccttt gaagaaagag gaacaatgct agatgcttct caaattgcaa
    12421 gcaaaaaagc agaaatcaat cctctcattc taccaacagt ggagccactt tcaacaagtg
    12481 gcaaaaaaga cagtgatttt tctaaggtgg cctattatca agctaagcgc aacttgactt
    12541 ataaaaacat tgaaaaattg ctacctttct acaacaaggc aaccatcgtc aaatacggaa
    12601 acctggtcaa tgagaacagt cttttatatc aaaaagaact cttgtcagca gtcatgatga
    12661 aggacaacca agtcatcaca gacattgttt ctaacaaaca gactgcaaac aaactcttgc
    12721 ttcactacaa ggatgattta tctgagaagc tggatctcaa ataccagaat gatttcgcca
    12781 aattagcaga atatagtctg ggcaatactg gacttctcta tacgccaaac caattcctgt
    12841 atgaccaaac ctctatcatc aagcaagtct tacctgactt acaaaaggtt gactatcatt
    12901 cagaagccat cagaaagacg ctgggtattt ctccaaacgt caagcaaact gagctctatc
    12961 tagaagacca gttcgccaaa acaaaacaac aactggaaga cagtttgaaa aaactcttgt
    13021 cagcggatgc tggactggct agtgctaacc ccgtcactga aggttatctt gtagataaaa
    13081 tcaaacgcaa caaggaagcc ttgctacttg gcttgaccta tctggaacgg tggtataact
    13141 ttagctatgg tcaggtgaat gtcaaagacc tagttctgta ccatttggac ttctttggta
    13201 aggggaatgc ttcaccatta gatactctga ttgagttggg taaatctggc tttaacaatc
    13261 ttctagctaa gaataatgtc gatacttatg gtatcagtct tgccagtcaa catggaacga
    13321 cagatttgtt tagcacgctg gaacattacc gaaaagtctt tttaccaaat acaagcaata
    13381 atgactggtt taaatcagag actaaggctt acattgtcga agaaaaatcc actatcgaag
    13441 aggtgaaaac gaagcaaggg ttagctggca ccaagtattc tatcggtgtt tatgatcgta
    13501 tcacgagtgc cacatggaaa taccgcaata tggtcttgcc tctcctgacc ttgccagaga
    13561 gatccgtatt tgtcatctcg accatgtcta gtctaggatt tggagcttat gatcgctacc
    13621 gcagtagtga ccataaagcg ggcaaggctc tcaatgattt tgttgaagaa aatgcgcgtg
    13681 aaacagccaa acgtcagcga gatcactacg attattggta tcgtatttta gacgacaatg
    13741 cacgtgaaaa actttataga aatattttgc tttacgatgc ttataaattt ggcgatgata
    13801 ataccgtagg gaaagctaca gaagtggcag attttgataa tccaaatcct gcaatgcaac
    13861 atttctttgg acctgttgga aataaagttg ggcataatca acacggtgct tatgctacag
    13921 gtgatgcagt ttattatatg ggttatcgaa tgttggataa ggatggagct attacttata
    13981 cgcatgagat gacacatgac tcagatcagg acatttatct tggaggatat ggtcgaagaa
    14041 gtggcttggg accagagttc tttgctaaag gattattaca agcaccagac catccagatg
    14101 atgcgaccat taccatcaac tccatcttga aacattcaaa atctgatagt acagaaagtc
    14161 gacgattaca agtacttgat ccaactacaa gatttaataa tgcagatgat ttgaagcaat
    14221 atgtccacaa catgtttgac gttgtttata tgttggaata tctcgaagga aattcaattc
    14281 ttaaattgga tacgaatcaa aaacaacaac ttcttagaaa agttacaaat gagtaccatc
    14341 ctgatcctga tggaaataag gtctatgcaa caaatgttgt cagaaatcta acagtagaag
    14401 aagttgaaag actacgttca ttcaatgatt tgattgataa taatattctt tcgtctaggg
    14461 aatatgcctc aggtaaatac gaaagaaatg gctacttcac tattaagtta tttgcaccga
    14521 tttatgctgc attaagtaat gatataggaa caccaggtga cctgatggga cgtcgtatag
    14581 cctatgaact actagctgct aaaggcttta aagatggtat ggtaccatat atctcaaacc
    14641 aatacgaaga agaagccaaa caaaagggca agacaatcaa tctctacggt aaaacaagag
    14701 gtttggttac agatgacttg gttttggaaa aggtatttaa taaccaatat catacttgga
    14761 gtgagtttaa gaaagctatg tatcaagaac gacaagatca gtttgataga ttgaacaaag
    14821 ttacttttaa tgatacaaca cagccttggc aaacatttgc caagaaaact acaagcagtg
    14881 tagatgaatt acagaaatta atggacgttg ctgttcgtaa ggatgcagaa cacaattact
    14941 accattggaa taactacaat ccagacatag atagtgaagt ccacaagctc aagagagcaa
    15001 tctttaaagc ctatcttgac caaacaaatg attttagaag ttcaattttt gagaataaaa
    15061 aatagtgtct actattagga aataaagttt aaaaaggtga tgaagaacaa accaagattc
    15121 aagcaggaat tcctactgat aatgaagtaa gttatgatct tatttatcag caggaaactc
    15181 ttcctgcaac aggttcatca acttctgagc ttacagcttt aggcctatta gctgttggta
    15241 gtttagttct tttggttcat aatatgacgg gaacagtttt ttgctccctc tgaaaagtca
    15301 tcatttgatg gcttttttct atatagggta aaagataggg taaaaggcta tcatcggaca
    15361 aaataaagaa ggcatgatat aatataaagt agatttctat gtcataaaac aagaactgtt
    15421 tggacatcat tcatttgaaa actctctatg ttcaaacaat agtaaaataa aataggggat
    15481 ctaaatcctt gctatgaaag gaaaaaactc aatggctact attcaatggt ttcctggtca
    15541 catgtctaaa gctcgtcgac aggtgcagga gaatttaaaa tttgttgatt ttgtgacgat
    15601 tttagtagat gcacgcttgc ctctatctag tcaaaatcct atgttgacca agattgttgg
    15661 tgataaacca aaactcttga ttttaaacaa ggccgacttg gctgatccag caatgaccaa
    15721 ggaatggcgt cagtattttg aatcacaagg aatccagacg ctagctatca actccaaaga
    15781 gcaagtgact gtaaaagttg taacagatgc ggccaagaag ctcatggctg ataagattgc
    15841 tcgccagaaa gaacgtggga ttcagattga aaccttgcgt actatgatta tcgggattcc
    15901 aaacgctggt aaatcaactc tgatgaaccg tttggctggt aaaaagattg ctgttgttgg
    15961 aaacaagcca ggggtcacaa aaggtcaaca atggcttaaa accaataaag acctggaaat
    16021 cttggataca ccggggattc tctggcctaa gtttgaggat gaaactgttg cacttaagtt
    16081 ggcattgact ggagctatca aagaccagtt gcttcctatg gatgaggtta ccatttttgg
    16141 tatcaattat ttcaaagaac attatccaga aaagctggct gaacgcttca aacaaatgaa
    16201 aattgaagaa gaagcgcctg tgattattat ggatatgacc cgcgccctcg gtttccgtga
    16261 tgactatgac cgtttttaca gtctcttcgt gaaggaagtc cgtgatggca aactcggtaa
    16321 ctatacctta gatacattgg aagacctcga tggcaacgat taaagaaatc aaagaattcc
    16381 ttgtgacagt caaggagtta gaaagcccta tttttttaga gcttgaaaag gataatcgct
    16441 caggagttca aaaggaaatc agcaagcgta aaagagccat tcaagctgaa ttagatgaaa
    16501 atttgcgctt ggaatccatg ctttcttatg aaaaagaact ttataagcaa ggattgacct
    16561 taattgcagg tattgatgag gttggtcgtg gtcctcttgc tggtcctgta gtcgctgcgg
    16621 ccgttatttt atctaaaaat tgtaagatta aaggtctcaa cgacagcaag aaaattccta
    16681 aaaagaaaca tctggagatt ttccaagccg ttcaagacca agccttgtcg attggaattg
    16741 gtatcataga taatcaggtc atcgaccaag tcaacatcta tgaagcaacc aaactagcca
    16801 tgcaagaagc aatctcccag ctcagccctc aaccagagca ccttttgatt gatgccatga
    16861 aactggactt gcccatttca caaacctcca ttatcaaagg agatgccaac tccctctcta
    16921 tcgcagcagc atctatagta gccaaggtaa cacgtgatga attgctgaaa gaatacgatc
    16981 agcagttccc tggctatgat ttcgctacta atgcaggata tggcacagct aaacatctgg
    17041 aaggcctcac aaaactagga gttaccccaa ttcaccgaac cagctttgaa cccgttaaat
    17101 cactggtttt aggtaaaaaa gaaagttaat tgaaaggaaa taacatggag gaacagtcgg
    17161 aaatagtccg ttctaagaaa gaattcgcct ttgcatccag cactatacta tcccaagttg
    17221 gtcgaggaat cattgtcggc ctcatcgttg gaattatcgt cggatccttt cgtttcttaa
    17281 ttgaaaaggg cttccacctg atacaaggag tttatcaaga tcaagggtac ttagtgcgca
    17341 atctttttgt actggttttg ttttatatac tcatctgttg gctcagtgcc aaactaacac
    17401 ggtcagaaaa agatattaaa ggctcaggaa ttcctcaagt cgaagccgaa ctgaaaggcc
    17461 tcatgtccct caactggtgg ggcattcttt ggaaaaaata tgtgctaggt attcttgcta
    17521 ttgccagtgg actcatgctg ggtcgagagg gacccagcat tcaacttgga gcagttggtg
    17581 gtaaaggaat tgccaagtgg ctcaaatcca gtccagtaga ggaacgttcc ttgattgcca
    17641 gtggagctgc agcaggttta gccgcagcct ttaatgctcc tattgcagca cttctctttg
    17701 ttgtagaaga agtctatcac catttttcgc gctttttctg ggtctcaact ctagcagcca
    17761 gcatcgtagc aaactttgtg tctctactca tgttcggttt gacaccagta ttggatatgc
    17821 cagataacat tcctcccatg accctagatc agtattggat atatctcgtc atgggaattt
    17881 tccttggatt ttcaggtttt ctctatgaga aagctgtatt aaacgttgga agagtttatg
    17941 acttgattgg tcaaaaaatc catttggata gggcttatta tcccatcttg gcttttatcc
    18001 ttatcatacc agtcggaatc ttcttacctc aaatcattgg tggcggaaat cagcttgtcc
    18061 tttctttaac tgaacaaaat tttagtttcc aagttttatt agcttacttt ttaatccgct
    18121 ttatttggag tatgattagc tatggaagtg gactgccagg aggaattttc ctccccattt
    18181 tagctcttgg ttctttgctt ggtgccttag ttggtgttat ctgtgtcaat cttggacttg
    18241 tcagtcaaga gcaattccct atatttgtca ttctaggaat gagtggctat tttggagcca
    18301 tatcaaaagc tcccttaacc gctatgatcc tcgtaactga gatggtagga gatattcgca
    18361 accttatgcc acttggtctt gtcactcttg tttcttatat tatcatggat ttgctcaaag
    18421 gtacgccagt ctatgaagcc atgctggaaa aaatgcttcc agaagaagta tctagcgaag
    18481 gagaagttac acttatcgaa ataccagttt ctgataaaat tgctgggaaa caagttcatg
    18541 aactcaactt accacacaac gtcctcatca caactcaagt ccataatggc aagagccaaa
    18601 cagttaacgg ctcaaccaga atgtatctgg gtgatatgat tcacctggtt attccaaaaa
    18661 gtgaaattgg aaaagtcaaa gatttgttgt tgtagtatga gtatttacat aatttatgtt
    18721 atgtaaatga tcagtttgat ttatttagaa aaccgattct caggaatgag atcggttatt
    18781 ttttactgat gaggaatttt acatataaat aattgaactt tattaaaaat aagactataa
    18841 ttaagttaga aatgataaag tataaagc
//
LOCUS       DAVHEX010000049        18744 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVHEX010000049 DAVHEX010000000
VERSION     DAVHEX010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18744
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.049"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(284..1090)
                     /gene="yidA"
                     /locus_tag="VVL45_001591"
     CDS             complement(284..1090)
                     /gene="yidA"
                     /locus_tag="VVL45_001591"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEU4187746.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDEGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGKYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYERYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
     gene            complement(1103..2407)
                     /locus_tag="VVL45_001592"
     CDS             complement(1103..2407)
                     /locus_tag="VVL45_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU4187747.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMTAALLHDLGHGAYS
                     HTFEHLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKDFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSHRFVNRKVFKSITFSQEDQDQLTSMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            2479..2856
                     /locus_tag="VVL45_001593"
     CDS             2479..2856
                     /locus_tag="VVL45_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEU4187748.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELVMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTSHYQVDRQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            2947..3279
                     /locus_tag="VVL45_001594"
     CDS             2947..3279
                     /locus_tag="VVL45_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEU4187749.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDDFLQEQIEI
                     LTSIDNRE"
     gene            3291..4862
                     /gene="ffh"
                     /locus_tag="VVL45_001595"
     CDS             3291..4862
                     /gene="ffh"
                     /locus_tag="VVL45_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEU4187750.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(5057..6340)
                     /locus_tag="VVL45_001596"
     CDS             complement(5057..6340)
                     /locus_tag="VVL45_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEU4187751.1"
                     /translation="MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLKANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLLGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(6643..7356)
                     /gene="rsmG"
                     /locus_tag="VVL45_001597"
     CDS             complement(6643..7356)
                     /gene="rsmG"
                     /locus_tag="VVL45_001597"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEU4187752.1"
                     /translation="MKPKTFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            7450..8010
                     /locus_tag="VVL45_001598"
     CDS             7450..8010
                     /locus_tag="VVL45_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEU4187753.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            8012..8911
                     /gene="htpX"
                     /locus_tag="VVL45_001599"
     CDS             8012..8911
                     /gene="htpX"
                     /locus_tag="VVL45_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEU4187754.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSGMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(8955..10514)
                     /locus_tag="VVL45_001600"
     CDS             complement(8955..10514)
                     /locus_tag="VVL45_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU4187755.1"
                     /translation="MHYEHSRKGNHMIKINHLTITQNKDLRDLVSDLTMTIQDGEKVA
                     IIGEEGNGKSTLLKILMGEALSDFTIKGNIQSDYQSLAYIPQKLPEELKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQEIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLSKKMKTVLSQEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLTKLKQLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYISKTGEKEELQKIHSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(10753..11070)
                     /locus_tag="VVL45_001601"
     CDS             complement(10753..11070)
                     /locus_tag="VVL45_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEU4187756.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQGTADDIRLQELLNTTMQELKKT
                     EKLNNSILIDLEKFYQPTSPLIGLGSLKLNNQARTAWRNYDKFHYEHVKHVLSLYGPV
                     FEF"
     gene            11240..11782
                     /locus_tag="VVL45_001602"
     CDS             11240..11782
                     /locus_tag="VVL45_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEU4187757.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNQLDQEVLDDD
                     LVLPIENGELDLAESVSDNILLNIPIKVLTAEEEAGQGFISGNDWQIMTEEEYQAQKA
                     VKKEENSPFAGLQGLFDGDE"
     gene            11782..12411
                     /gene="nth"
                     /locus_tag="VVL45_001603"
     CDS             11782..12411
                     /gene="nth"
                     /locus_tag="VVL45_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEU4187758.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            12622..13143
                     /gene="pyrR"
                     /locus_tag="VVL45_001604"
     CDS             12622..13143
                     /gene="pyrR"
                     /locus_tag="VVL45_001604"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEU4187759.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            13162..14085
                     /locus_tag="VVL45_001605"
     CDS             13162..14085
                     /locus_tag="VVL45_001605"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEU4187760.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGAELFFAGPEEWRSQEFADYGKFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            14135..15214
                     /gene="carA"
                     /locus_tag="VVL45_001606"
     CDS             14135..15214
                     /gene="carA"
                     /locus_tag="VVL45_001606"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEU4187761.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            15526..18702
                     /gene="carB"
                     /locus_tag="VVL45_001607"
     CDS             15526..18702
                     /gene="carB"
                     /locus_tag="VVL45_001607"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEU4187762.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTRLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAIAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDKVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKNVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
BASE COUNT         5453 a         3602 c         4218 g         5471 t
ORIGIN      
        1 tgaagtatcc taatttgtat aattgattgt gtgtagattc agggtcaatt tgaagagagt
       61 atttaaaata tcttgggaaa ttataatttc tccctgtttt ttgagtccaa tctatgagta
      121 tgagttgccc aggagtaagg ccacattcta attcgtttag gtaaaaattg ttacaatcag
      181 aatttgtgtc tctattatga ttattttgtt ttaaattaaa tagtttgcta aaaaaattca
      241 ttggatttta ttgctaatca attcttattg aaaaatgata cttttacagt acccatgttc
      301 ggatggcatg ggcaacgccg gattcgtcat ttgttttggt gatgtatttg gcgatttttt
      361 tgatttctgg atttccattt tccatgacaa cggggtttcc aacgacttcc agcatggcac
      421 ggtcattttc ttcatcaccg attgccatgg tttcatcttt ggtcaatccg agtttttcag
      481 ccaagtgagt aatggctgaa cccttgtcta cattcttttt aaggagttcg aggtagaaag
      541 gagcagattt gttgatggag tagcgctcgt aaaattctgc tggaattttt tcaatcgcag
      601 catcgagaat ttctggttca tcgataaaca tacatttaac aatttctttg ccagccattt
      661 cttcaggggt acggtagaag ataggcatgc tgacgagggt tgattcgtgt acagtgtatt
      721 ttccgatatt gcgatttgca gtatagatac cgtccttggt aatggcatgc atgtggacac
      781 cgagcttgcg actgaggaat tccatatcta gataatcctc ataagtcaag gattcgctga
      841 taatctcatg tcctgtagca gtttcttgga caagggcacc gttgaaggtt accacatagt
      901 ccccctcgtc tctcaactgc aagtcgtcta gaagtttggc aacgcctgcg atagggcggc
      961 cagttgcaat cacgactttg acaccagctt ctttggcatc ttggatggca gaaaaaactt
     1021 caggagtgat ttccttttgg ctgttgacaa gggttccgtc gatatcaacg gcaattagtt
     1081 taatactcat aaatttcctc ttctagtttt tatttggggt aaaatgatcg ttctcaatca
     1141 agtgtaaaaa ttgctgggta atgcttgcaa agatgctgtt ttggtccaac atttcttttg
     1201 gaaaataaaa gcgattatct ccgtggcgac tgccagcaag ggattggacg ataggagaca
     1261 ggctagagag ttcggccagt tctccatttt tttgtaaaat ctcaatctgt gtccgtgggt
     1321 tttcagattc gggacgatag atatcataag ggaggtcaaa gttcttatga atggcagtgt
     1381 agtagtcggg atcaaagccg atatcctcaa ccaattttct catgctagta agttgatctt
     1441 ggtcctcttg tgaaaaggta atggatttaa agaccttgcg gttgacaaag cgatgcgata
     1501 aatctgcaag aatcttgtca ggactggtca tccaaagctg gaagtaggta ttcatcacgc
     1561 catcatccag agccagatag tcagtcaagg tcacattttt ttcgaagaaa ggcaggaggt
     1621 gtggagaagt tcgggcaaag aaatccttgt cctcaggata gagttccttg gcgcgtttga
     1681 gaagattctg taggagaact tccatggcgc gtgttgcggg gtggaaataa acctgcatgt
     1741 acatctggta gcgactgagg acgtagtctt cgatggcgtg catgccattg cgctgaaagg
     1801 cgataccatt ttcgatagga cgaatgactc ggaggattcg agtcaggtca aattccccat
     1861 aggatgctcc tgtaaaatag gagtcgcgca agagatagtc catgcgatct gcgtcaatct
     1921 gactagaaat gagctgcacg acctgcttat taggataggt atggtcaatg acactggcca
     1981 ccttttctgg gaaatcaggt gccacttgta gcaggacttg gtgaatctct gtctcaggat
     2041 tttgaataat ctcctgagta atggcttcat ggtctgtatc aaagagatgt tcaaaagtat
     2101 gggagtaggc accatgccca aggtcgtgta ggagagcagc ggtcatggtc aagagagact
     2161 cggcaggatt ccattcctca ggatattttt cttcgaaaat ctctgtgatg cgtcgtgcaa
     2221 tttcatagac tcctagacag tgagagaagc gactgtgttc tccaccgtgg aaggtataac
     2281 tggaagttcc cagttgtttg atccggcgca aacgctgaaa ttcttttgta ttaatcaagt
     2341 catagatgat ttgattattg acatggatgt agttgtgaac agggtcacgg aatacttttt
     2401 cgttcatatg accattatag caaaatcctg ctctttttgg taaaatagta ggacaagaga
     2461 caagaaagag ggcctgatgt gaagattcgg atgcgaaata cgattcagtt tgatgagcag
     2521 ttggaagtga ttgaccagct ttatgatgtg gaagtgcatg aaaaaggaga ttatagctac
     2581 ctgcttttct ataatgagga aaaggaaaaa gtagtgatta aatttcatgg tcaagaactt
     2641 gtgatgagcc gattttctaa tcccaagacc attatgcgct ttctaaagga tagcgatagt
     2701 ttagcctata ttcctacacc tatgggcatg caggagttta tcatccaaac gagccattat
     2761 caagttgata ggcaaaagat tgaactagat tatcaactac aaaatcaaga gggacatccc
     2821 tttgccagct atcaattgga aattacttgg ggctaggctc atgtcttttt caaatttagc
     2881 tatctatctt cttggatcgg cttcctagtg tggtagatta ggctaatttt tggtataata
     2941 aaagttatgg aaatcgaaaa aaccaatcgt atgaatgcgc tctttgaatt ttatgcggcg
     3001 cttttgacag ataagcaaat gaattatatc gagctctact acgctgatga ttacagcctt
     3061 gctgaaattg ccgaggagtt cggtgtcagt cgtcaggctg tctatgacaa tatcaagcga
     3121 acagaaaaga ttctggaaga ttatgagatg aaattgcaca tgtactcgga ctatattgtc
     3181 cgcagtcaga tttttgatca gattttggag cgctatccca aggatgactt tctgcaggag
     3241 cagatagaaa ttttaacaag cattgataat agagaataag aggaagaaaa atggcatttg
     3301 aaagtttaac agaacgtttg cagaacgtct ttaaaaatct acgtaaaaaa ggaaaaatct
     3361 ctgaatctga tgtccaagag gcaaccaaag aaattcgctt ggccttgctc gaggccgacg
     3421 ttgccttgcc tgttgtaaag gactttatca agaaagttcg tgagcgtgca gtcgggcatg
     3481 aggtcattga tacacttaat cctgcgcaac agattattaa aatcgttgat gaggaactga
     3541 cagccgtttt aggttctgat acggcagaaa ttatcaagtc acctaagatt ccaaccatca
     3601 tcatgatggt tggtttacaa ggggctggta aaacaacctt tgctggtaaa ttggccaaca
     3661 aactcaagaa agaagaaaat gctcgtcctt tgatgattgc ggcggatatt tatcgtccag
     3721 ctgccattga ccagcttaag accttgggac aacagattga tgtgcctgtc tttgcacttg
     3781 gaacagaagt accagctgtt gagattgtac gtcaaggttt ggagcaagcc caaactaatc
     3841 ataacgacta tgtcttgatt gatactgcgg gtcgtttgca gattgatgag ctcctcatga
     3901 atgagcttcg tgatgtgaaa gcattggctc aaccaaatga aatcttgctt gtcgttgatg
     3961 ctatgattgg tcaggaagca gccaatgttg cgcgtgagtt taatgctcag ttggaagtga
     4021 ctggggtcat ccttaccaag attgatggcg atactcgtgg tggtgctgct ctgtctgttc
     4081 gtcacattac tggaaaacca atcaagttca ctggtacagg tgaaaagatt acggacattg
     4141 aaaccttcca cccagaccgc atgtctagcc gtatccttgg tatgggggat atgctcactt
     4201 tgattgagaa agcttctcag gaatacgatg aacaaaaagc ccttgaaatg gctgagaaga
     4261 tgcgcgaaaa cacctttgat tttaatgatt tcatcgatca attagatcag gtgcaaaata
     4321 tggggccgat ggaagacttg ctcaagatga ttccaggtat ggccaacaat ccagcccttc
     4381 aaaacatgaa ggtggatgaa cgccagattg ctcgtaaacg tgccattgtg tcttcgatga
     4441 cacctgaaga gcgtgaaaac ccagatttgt taaatccaag ccgtcgccgt cgtattgctg
     4501 ctggttctgg aaatacattc gtcgaagtca ataaattcat caaggacttt aaccaggcta
     4561 aacagctcat gcagggtgtt atgtctgggg atatgaataa aatgatgaag caaatgggga
     4621 ttaatccaaa taaccttcct aaaaatatgc caaatatggg aggaatggat atgtctgccc
     4681 ttgaaggaat gatgggacaa ggcggtatgc ctgacttatc agctctcgga ggagcaggaa
     4741 tgccagatat gagccagatg tttggtggcg gtttgaaagg taaaattggt gaatttgcca
     4801 tgaaacagtc catgaaacgt atggctaaca aaatgaagaa agcgaagaag aaacgcaagt
     4861 aagacaagag attgatgcaa atctcatttg atgagaaaat aaagtaaaaa tttctatgat
     4921 aaaacgtaca gtatcaaggt tttagaatat ctgatattat acgttttttg attttaataa
     4981 ttttgttaag acacttttat tatggtatac gaaaaatcca ctcagctgtc tgagtggatt
     5041 aggtgtattt agactcttag tctttatttt cgtatggcaa gatcaagttc aagatgattc
     5101 ctgtcatggc tgaaagggca gtacctgaaa gtgtaactgg accaagttta aggatagctc
     5161 ctccaagtcc aagaaccaac atagcacttg cgatgatgag gtttcgcatt tgagcgaaat
     5221 caacacgttc tttaatcaag actttcaaac cattgctggc gataacccca tagagaagga
     5281 ttgacatacc accaagtaca gcgtttggaa tagttgaaat caaggcagtg aatttaccaa
     5341 ggaagctgag ggcaatcgcg atgaaggcag cgttacggat aactgagaca gaagcgatac
     5401 gagtcatacc gataacccct gtattttctc cgtaagttgt attggctggt ccaccaagga
     5461 aggcagaaac agaagttgcg ataccgtcac caagaagagt acggtgaaga cctggttctt
     5521 ttaagaattg acgaccacag atttgaccca aaacagtatg gtctccgata tgttcagaaa
     5581 ttgttacgat agcgattggc aagatagcga tggcttctgg accaaagtaa agattgtact
     5641 ctttaaaggc accacctgtg ctaaatggca agtagaaacc aggaatttcg aaccagttgg
     5701 ctttaagaac tggtgtaaag tcaaccaagc caagagttag tgcgaaaagg taaccaccga
     5761 taatggcaaa gaggaatgga atgattcgta ggaagccttt tccttttgta ttgataaagg
     5821 cagcaattag gaaagtaaca acggctacca gagcattttt ccaatttccg tctgctacaa
     5881 gacctgcatt ggtaacagct gaacctgcaa gtccaagacc gataacgatg atcataggac
     5941 cgataatgat tggtggcaag agtttatcaa tccattttgt tcctacaaat cggatgctgg
     6001 tagcaacaag gacatagacc aaaccagtca agataacccc tgtttgggca gcagatacat
     6061 ccccccccat ttctttcata gccagtgaca tagctgtgat aaaggcaaat gaagaaccta
     6121 gataaactgg aactttaaaa ccagtagcaa tcatgtagat gagtgttcca acacctgaag
     6181 caaaaagggc aacagataca ggcattccca aaatcaatgg taccaagatg gtcgcaccaa
     6241 acatggcgaa aacgtgttgg aaactaagga gaattccttt tccagccgaa ggacgttggt
     6301 caacgtctag taacaaatca acagttgatt cttgtttcat ataaacctca cttttgggca
     6361 tcaaaaaaga gcccattatt ttacagcaat aggccctcag agtaagactt ctttaaaatt
     6421 tagattttaa agagttttaa atatttctgc gtcttcgtca cctcacggga tgacattaaa
     6481 aacctttgct taggatagta tagcagaaaa aaatgatttt gtaaatcatt ttttcccgag
     6541 cctagaaata aaagagcgag gctgattttg taaatcattt tttcccgagc ctagaaataa
     6601 aagagcgagg ctgattttgt aaacatttgt ttactaaaaa atttaaagtg ggcgtttatt
     6661 tggcatacca gccttacgcg gatatttatt tggtgtttct tttttctttt ctaccactgt
     6721 gatatagcgc ggatctctat tcggtagggc gtagctgaga ttgtcttcga ccttactaaa
     6781 aaggagattg agggcattct tagcttctaa taattcctca ggcgcattgc tggccttgag
     6841 tgccaatagt tttccaccaa ccttaaggta gggaatggtc aattcagata ggacctgcat
     6901 acgggcaacc gcacgagctg ttacaaaatc atattgagca cggaagttct tgtcttgggc
     6961 aaaatcttcg gcacgtccgt ggtagaaatg aactccgttc aaatccagtt cttgagccaa
     7021 gagttgtagg aagttgatgc gcttattgag tgaatcaata atggtcacat ctaactccgg
     7081 atagagaatt ttcattggta gactaggaaa tcctgcccca gccccgatat caagaagttt
     7141 gatagtttca ttgggaatca aaccttgaag aatgggtgca atcgaatcgt aaaaatgttt
     7201 gagataaact tcttccttgt ccgtaatcgc cgtcaaatta atcttctcat tccactcgac
     7261 caagagctca aaataacgtt caaattgttc tttttgctgg tccgaaagtg gaagattctg
     7321 ctcggcaagc aagttgtaaa atgtttttgg tttcatagtt atttccttct ttagtatcat
     7381 cttattttac cacaatcata gaaaatttga tatactggta ctaatctaaa agcagaaagg
     7441 acttatatta tgacttggat tattcttgga gttatcgctc ttattgttat ttttgtgatt
     7501 gttagctata acggtttggt taaaaatcgt atgcaaacca aggaggcttg gagtcagatt
     7561 gatgttcagt tgaaacgtcg aaatgacctc ttgccaaact tgattgagac tgtaaaaggt
     7621 tatgccaaat atgaaggttc tacccttgaa aaggtggcag aactacgtaa ccaagtggcg
     7681 gcagcgactt caccagcaga agctatgaaa gccagtgatg ccctcactcg tcaggtttca
     7741 ggtatttttg cagttgcaga aagctatcca gatttgaaag ctagtgctaa ctttgttaaa
     7801 ttgcaagagg agttgacaaa cacagaaaat aaaatttctt actctcgtca actctataac
     7861 agtgttgtca gcaactacaa tgtaaaatta gaaactttcc cgagcaatat tatcgctgga
     7921 atgtttggat ttaaagcggc agatttcctt caaacacctg aagaggaaaa gtcggttcct
     7981 aaagttgatt ttagcggttt aggtgactaa gatgttgttt gatcaaattg caagcaataa
     8041 acgaaaaacc tggattttgt tgctggtatt tttcctactc ttagctcttg ttggttatgc
     8101 ggttggttat ctctttataa gatctggact tggtggtttg gttattgcac tgattatcgg
     8161 ctttatctac gctttgtcta tgatttttca atcgacagag attgtcatgt ccatgaatgg
     8221 agcgcgtgag gtggatgagc aaacggcacc agacctctac catgtagtgg aagatatggc
     8281 tctggtcgct cagattccta tgccccgtgt tttcatcatt gatgatccag ccttaaatgc
     8341 ctttgcgaca ggttctaatc ctcaaaatgc ggctgttgct gcgacttcag gtctactagc
     8401 tatcatgaat cgtgaagaac tagaagctgt tatgggacat gaagtcagtc atattcgtaa
     8461 ttatgatatc cgtatttcga ctattgcagt tgcccttgct agtgctatca ccatgctttc
     8521 tggtatggca ggtcgtatga tgtggtgggg tggagcaggt cgcagacgaa gtgatgatga
     8581 ccgagatgga aatggtcttg aaatcattat gctagtggtt tccctactag ctattgtact
     8641 ggcacctctc gctgcaacct tggttcagct cgctatttct cgtcagaggg aatttctggc
     8701 agatgcatct agtgttgagc tgactcgcaa tccccaggga atgattaatg ccctagataa
     8761 gttggacaat agcaaaccta tgagtcgcca cgtcgatgat gctagcagtg ccctttatat
     8821 caatgatcct aagaaaggtg gggggttcca aaaactcttt tatacccacc cacctatctc
     8881 agaacggatt gaacgtttaa aacagatgta aaagaatcca gaggtcgtct ctggattttt
     8941 gctatgttgc aaatttataa atcttctaaa ttaactagct tcaaaccgtg ttctgtcatg
     9001 cgatagatga tcgagcagac ttcttttaag aaacgacggt catgcgaaac agtgatgaga
     9061 ccgcctggat aggttgcaaa gagttttctg atttggggtt gagaagtggg agaaaagttt
     9121 cgtgtgggtt catccagcag gagaaagttt ggtttgcgca ggactaaatc caaaagcagg
     9181 agttttccct gttgtccgcc agataaggag cgaatttgat gctgcatttc tggataactg
     9241 aaattgagac tggctaggtg agagtggatt ttctgaagtt cctctttttc cccagttttg
     9301 ctgatataag ctattggtga taaatccaat tgtagttttt tgtggtaatc ttgtggcata
     9361 aaaccaaggg aaatctctct tttatcattc agaagttgct ttaatttggt taacagagtt
     9421 gatttcccaa caccatttgg cccgataata ccgatttttt cttgcccacg gacagttagt
     9481 tgtagttttt gaaccaaaac tcggtcgtca atggacaaat tttctttttc cagttggact
     9541 aagactttag aagctggtaa tggttggatg tctgaaaaga aaagttggat ttgttcctct
     9601 tcaagtggtt tttgagtcat ggactgagct gccttttcgt agcgtttttc ttgtgagagg
     9661 acagttttca tctttttaga caataggcga ccggcagtac tatctttggt agctcgaagc
     9721 gcagtttcta cattttgctt aactctccga tgtttttcca tggttttatc gtaggctctt
     9781 tggttgttag cagcttgctg actttgtttg gcaaaattag cctttctctg ctcactatag
     9841 ctatcatagt ctaaatgctc tactagcgtt tccgcttctt tacggtgttt gaccagtcgc
     9901 aagtgaacaa tagtgtctgc cgtttcagaa agaaagtctt catcatggga aatgaaaata
     9961 acggtttgcc tggtcttttg aatctggcct tttagccaat caactgtctc aaggtctagg
    10021 tcatttgaag gttcatctaa aaatagaatc tcaaagggtt tggctaactc atggataagc
    10081 tgaattttca aagcttctcc ccctgataga ttgccaatct cttggtcact tgcgaaacga
    10141 ttgctatcaa aatgcaattc ctccgccaaa cgatagagga tactgtagtc taaatcaata
    10201 gaatctaaaa agaagtagtc gtgtaaagtt ttctttttca gctcctcggg aagtttttga
    10261 ggaatgtagg ccagtgactg atagtcagat tggatgtttc ccttgatagt gaaatcagac
    10321 aaagcttccc ccattaaaat tttaagtaag gttgatttgc catttccttc ttcaccaata
    10381 atagcaacct tttccccgtc ttggatggtc atggttaggt cagatacaag atctcgtaaa
    10441 tctttgtttt gtgtgatggt tagatgattg atttttatca tatgatttcc ctttctagaa
    10501 tgttcatagt gcataacaaa aagctctact ttacctagta gagcccaaag tctatgtcaa
    10561 aaaaactcta ttaacatgcc agaaaaagag cataaaagag cacacaaaaa gagacaaaaa
    10621 caagatctac taaataaagg ttctttattt gatagactta gttgttcatg tctcccttac
    10681 ctccgagtat tagtatcttt atattatacc tttaaggaaa ttttgtcaac agaaaactgg
    10741 aaattctatg ctctaaaatt caaaaacagg tccatagaga cttagtacgt gtttgacatg
    10801 ctcgtaatgg aatttatcat agtttcgcca agcagtgcgt gcttgattgt ttagttttag
    10861 gctacccagt ccaatcagag gactggtggg ttggtaaaat ttctcaagat caattaagat
    10921 actgttgttc aacttttccg tttttttgag ctcttgcata gtagtgttta gcagctcttg
    10981 tagtcgaata tcatctgccg taccctgttt acagttttgg tagcttttat ttaacttgga
    11041 taggagttga atgagtttct tgtcttccat gtgagcatcc tccttgatct gatgtactct
    11101 ttccctatag tatagcacta aaaaacaaaa ctatcatggt aaatatgtta aaatttcgaa
    11161 ttttaattac aaagtttggt gtgctgattt gtagcctttt catggtataa tcaagagagt
    11221 aaatctttat ggaggaagta tgaaattaaa tattcaagaa attcgtaagc agtctgaagg
    11281 tttgaacttt gaacaaacgt tagatttagt tgatgacctg cgtgcacgta atcaagaaat
    11341 tttagatgta aaagatatcc ttgcagttgg gaaagtacaa tatgaagacc gtatgtattt
    11401 cttagattat caactatctt ataccattgt tcttgcttcg agtcgcagta tggagccagt
    11461 tgagttagtt gaatcttatc cagtcacgga agttttcatg gaaggcgcaa ctaaccagct
    11521 agatcaagaa gttttagatg atgacttggt cttgcccatc gaaaatgggg agcttgacct
    11581 tgctgagagt gtatcagaca atatcctgct aaacattcct atcaaggtct tgacggctga
    11641 agaagaagct ggtcaaggat ttatctcagg aaatgactgg caaatcatga cagaggaaga
    11701 ataccaagct caaaaagcag taaagaaaga agaaaacagt ccttttgctg gcttacaagg
    11761 actatttgac ggagatgaat aatggtcttg tcaaaaaaac gagcacgaaa ggtgctagaa
    11821 gaaatcattg ccctcttccc agatgccaag cctagtcttg attttaccaa tcattttgaa
    11881 ctcctggttg cggtcatgtt atcagcccag acgacagatg cagcggtaaa taaggccaca
    11941 ccaggtctct ttgttgcctt tccaacaccc caagctatgt ctgtagcgac agagagtgag
    12001 attgcttcac atatttctcg cttgggattg tatcggaata aagctaaatt ccttaaaaaa
    12061 tgtgcccaac agttactaga cgattttgat ggtcaagtcc ctcagacacg tgaagaattg
    12121 gagagtttgg caggtgttgg tcgcaagaca gccaatgttg tcatgagtgt aggatttggg
    12181 attccagcct ttgcagtgga tactcatgtg gagcgtattt gcaaacacca cgatattgtc
    12241 aaaaaatcag cgacgccact tgaggtggaa aagcgggtca tggatatctt gccgcctgag
    12301 cagtggttag ctgcccatca ggccatgatt tattttggaa gagccatttg ccatccaaaa
    12361 aatccagagt gtgaccagta cccacaatta tatgatttta gcaatttgta agatgctaca
    12421 ttgaaacttt ttgcttgatt ttaagcatgc tttgtgctat agtaaatgat aactaaatat
    12481 tcctttaaac ctgtcccgtg aggcaggcaa ggagcgcgat aaagtagaag ggtgaagtct
    12541 atactgtgtg cagtagggct cgcttggcta taaatttcaa gtctccttgt ttaaggagac
    12601 ttttcttttt ggaggtttgt catgaaaaca aaagaagttg tagacgaatt gaccgtcaaa
    12661 cgagcgatta cgcgtattac ttatgagatt atcgaacgca acaaagattt gaataagatc
    12721 gtcttggctg gtattaaaac tcgtggtgtc tttattgccc accgaatcca agaacgtttg
    12781 aagcagttag aaaatctttc agttcctgtt gtggaattgg atactaaacc tttccgtgat
    12841 gatgttaaaa gtggagaaga tacttctttg gtttctgtcg atgtgacaga ccgcgaagtt
    12901 atcttggtgg atgatgtgct ctatacaggt cgtaccatcc gtgctgctat tgataatatt
    12961 gtcggtcatg gtcgtcctgc gcgtgtgagt ttagcagttc tagtcgatcg tggacataga
    13021 gaattgccaa tccgtccaga ttacgttgga aaaaatatcc caaccagtcg ttctgaagaa
    13081 atcatcgtag agatgacaga acttgatgac caagacagag ttctgattac tgaagaagct
    13141 tagaaagtta aaggagtagc catgtcagaa aatcaacaag cattgaacca tgtggtgtcc
    13201 atggaagacc tcactgtcga tcaagtaatg aaattgatca agcgaggaat tgagtttaaa
    13261 aatggagccc agcttcccta tgaagaccat ccgattgttt ccaatctctt ctttgaggat
    13321 tctacacgga cacataagtc ctttgaagtc gcagagatta aacttggatt ggaacgactt
    13381 gactttgatg tgaagactag ttcggttaat aagggtgaga cactttatga caccattttg
    13441 actctgtctg ctttaggagt ggatgtctgt gtgattcgcc atccagaggt cgactactat
    13501 agagaattga ttgctagtcc aacgattacg acttccatca tcaatggtgg agatggttcg
    13561 ggccaacacc ctagccagag cttgcttgat ttgatgacca tttatgagga atttggccac
    13621 tttgagggtc ttaaagttgc tattgcaggt gacttggacc actcacgcgt tgccaaatcc
    13681 aatatgcaga ttttgaaacg cttgggagct gaactctttt tcgctggacc tgaggaatgg
    13741 agaagtcaag agtttgcaga ctatggaaag tttgtaacca ttgatgaaat cattgatcag
    13801 gttgatgtca tgatgtttct ccgtgtgcaa cacgaacgcc atgatagtgg agctgtattt
    13861 tcaaaagaag actaccatgc ccaacatggc ttgactcaag aacgttacga tcgtttgaaa
    13921 gaaacagcaa tcctcatgca cccagctcca atcaatcgtg atgtagaaat agcagaccac
    13981 ttggttgaag caccaaaatc acggattgtc caacaaatga ccaatggtgt ctttgttcga
    14041 atggcaatct tagaatccgt actagcgagt agaaacgcca actaaaacac aagacgttaa
    14101 aagacgccag tgagcgtcca cgagaggtga aaatatgaca aaaagaattc tagtattaga
    14161 agatggcaca gtttttgaag gcaaggcctt cggagcagat attgatgtaa caggcgaaat
    14221 cgtctttaat acagggatga ccggctacca agaatccatt acagaccagt cttataatgg
    14281 acaaatcttg acctttactt atcctttggt aggaaattat ggtattaatc gtgatgatta
    14341 tgaatccatt attccaactt gtaagggagt cgttgttttt gaagaagcgc gtagagctag
    14401 caactggcgc aaccaaatga ccttggatga atttttgaaa gccaagaaaa ttccagggat
    14461 ttcaggaatt gatacgcgtg ctcttaccaa gattatccgt aagcatggta ctatgcgtgc
    14521 aaccttgacc catgttgggg acagtatgga ccatgtgacg gaccagctcc aagcaacagt
    14581 tttaccgaca gacaatatca aacaggtttc tactaaaacg tcttatccag ctccaggagt
    14641 tggtttgagc gtggtgctag tggactttgg tctcaagcac tcaatcctac gtgaactttc
    14701 taaacgcaac tgtaacgtga cagttgttcc ttattcgaca acggctgaag aaattctcca
    14761 tctcaatcct gatggagtta tgttgtcaaa tggtccaggt aacccagaag acgttccaca
    14821 agcactcgac atgattcgcg gtgtacaagg aaaaattcca atctttggta tttgtatggg
    14881 acaccaactc tttgcaatgg caaacggggc taagacctat aagatgaagt ttggccaccg
    14941 tggattcaac catgcggtac gtgaaatcgc aacaggacga gtagacttca ccagccagaa
    15001 ccatggttat gcagtcagcc gcgaggattt gccagagcac ttgattatta cccacgaaga
    15061 aatcaatgac aagtcagtgg aaggtgtgcg ccacagatac caacctgctt tctctgttca
    15121 ataccaccca gatgcagctc ctgggccaca cgacgctagc tacctatttg acgagtttat
    15181 cgagatgatg gaagttttta aacaatcaaa ctaagaacga gtaaaagctc gtcggagaat
    15241 ttatgtaact gaacacggac ggaaagctca gaatgtagag aaaccaactt ggattgtcaa
    15301 tccttccttg attttctaaa attcaatcgc tttctattcg tcctttgtat cttatttaat
    15361 gtcgccctga gaggcgacga atagaaagga gaagatacat tgttcgcgga agtgaacacg
    15421 ccctaagaac tgtgtgaaaa agataaacat cgtcttgacg ctgaaggcgt ctgcgccgag
    15481 tttcctattt tcactgtgtt ctttacgggc tttgtatcat aaactatgcc taaacgtact
    15541 gatattcaaa aaattatggt gattggttct ggtccgatta ttattggtca ggctgctgag
    15601 tttgactacg ctgggaccca ggcttgcttg tcgttgaaag aggaaggtta tgaggttgtt
    15661 ttggttaact caaatcctgc aaccatcatg acggacaagg aaattgctga taaggtttac
    15721 attgaaccga ttacacttga gtttgtgaca cgtattcttc gtaaggaacg tccagatgcc
    15781 ttgctaccaa cactcggtgg tcagacaggt ctcaacatgg ccatggaatt gtctaaaaat
    15841 ggtatcttag atgagcttgg tgtagagctt ctcggtacta gattgtctgc cattgaccaa
    15901 gcggaggacc gtgacctctt taaacaattg atggaagagc ttgagcaacc cattccagaa
    15961 tctgaaattg ttaacacagt agaagaagca attgcctttg cagcgacaat tggctaccca
    16021 gtcatcgttc gtccagcctt tacccttggt ggtactggtg gtggtatgtg tgccaacgag
    16081 aaagaattgc gtgaaatcac tgaaaatggt ttgaaattgt cacctgttac ccaatgtttg
    16141 attgagcgtt cgattgccgg tttcaaggaa attgaatacg aagtgatgcg tgactcagct
    16201 gacaatgcct tggttgtttg taacatggaa aactttgacc cagttgggat tcacacaggg
    16261 gattccattg tatttgcccc tgcgcaaacc atgtcagact atgaaaacca aatgcttcgt
    16321 gatgcgagct tgagcattat tcgtgccctc aagattgaag gtggatgtaa tgttcagcta
    16381 gctcttgatc ctaacagctt caagtattat gttatcgaag taaaccctcg tgtatcacgt
    16441 tcgtcagccc ttgcttctaa agcgacagga tatccgattg ctaagttggc tgccaagatt
    16501 gcagtaggtt tgaccttgga tgaggttata aacccagtta caggttcaac ctatgccatg
    16561 tttgaaccag cccttgacta cgtagttgct aagattccac gtttcccatt tgacaagttt
    16621 gaaaagggtg aacgccgtct cggtacacaa atgaaggcga ctggagaagt catggcaatc
    16681 ggtcgtaaca tcgaggaatc tcttctcaag gcctgccgct cccttgaaat tggggtgcac
    16741 cacaatgaaa tacctgaact tgcagcagtt tctgatgatg ccttgattga aaaggttgtc
    16801 aaagcccaag atgaccgtct cttctatgtg tcagaagcca ttcgccgtgg ttacacacct
    16861 gaagaaattg cagagcttac aaaaattgat atcttctatc ttgataaact cttgcatatc
    16921 tttgaaattg agcaagaatt gggtgctcat ccacaagatc tagaagtttt gaaaacagcc
    16981 aaacttaatg gtttttcaga ccgtaagatt gctgaactct ggggaacgac tgatgataaa
    17041 gttcgccaac ttcgcttgga aaacaagatt gttccagttt ataagatggt agacacttgt
    17101 gcggcagagt tcgactccga aacgccatat ttctattcaa cctatggttg ggaaaatgaa
    17161 tctatcagat ctgataaaga atctgtactt gtcctaggtt caggtccaat ccgtatcggt
    17221 caaggggttg agtttgacta cgcaaccgtt cactctgtta aggctatcca ggcagctggt
    17281 tatgaagcta tcatcatgaa ctcaaaccca gagaccgttt caacagactt ctctgtatca
    17341 gataagcttt actttgagcc attgacattc gaagatgtta tgaacgtcat tgacttggaa
    17401 caaccaaagg gagttatcgt tcagttcggt ggtcaaacag ccatcaacct tgcggagcca
    17461 ttggcaaaag caggtgtgac catccttggt acacaggttg ctgacctaga ccgtgccgaa
    17521 gaccgtgacc tcttcgagca agctctcaaa gaattggata ttccacagcc accaggacaa
    17581 acggctacca atgaagaaga agcagctctt gcagctcgta agattggctt cccagttctt
    17641 gtccgcccat cttatgtact tggtggacgt gccatggaaa tcgttgaaaa cgaagaagac
    17701 cttcgttctt acatgcgaac tgctgttaag gctagtccag accacccagt tcttgtcgac
    17761 tcttatatcg ttgggcaaga gtgcgaagtt gatgccattt cagatggtaa aaatgtactc
    17821 attcctggta tcatggagca tatcgaacgt gctggtgtcc actcaggtga ctcaatggct
    17881 gtttacccac cacaaacctt gtcgcaaaag gttcaggaga ccatcgcaga ctacaccaaa
    17941 cgcttagcaa tcggtcttca ctgccttgga atgatgaaca tccagtttgt tatcaaggat
    18001 gaaaaagtct acgttattga ggtcaatcca cgtgccagcc gtaccgttcc attcctatct
    18061 aaggtaacca atattcctat ggctcaagta gcgaccaagc tcattcttgg tcaaagttta
    18121 tcagaacttg gctaccaaaa tggactttac cctgaaagca cccgtgttca tatcaaggca
    18181 cctgtcttct cctttaccaa actagctaag gtagacagct tgctcggtcc tgaaatgaag
    18241 tcaacaggtg aagttatggg ttctgatgct actttggaaa aagctctcta taaagccttt
    18301 gaagcttctt atctacactt gccaactttt ggtaatgtcg tctttaccat cgctgatgat
    18361 gccaaagaag aagccttgaa cttggctcgt cgtttccaaa acattggtta tggaatcctt
    18421 gcgacagaag ggacagcagc cttctttgct agtcatggct tgcaagccca acctgttggt
    18481 aagattggtg atgacgataa ggatatccca agttttgtac gcaaaggaag aattcaagct
    18541 atcattaaca cagtaggaac aaaacgaact gctgacgaag atggtgagca aattcgtcgt
    18601 tcagccattg aacacggggt gcccctcttc acagcgctag atacagctaa tgctatgctt
    18661 aaggtacttg aaagtcgtag ctttgttaca gaagcgattt aaatagaaaa ataagatata
    18721 gcaaactgaa tttagaacag taca
//
LOCUS       DAVHEX010000050        16769 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVHEX010000050 DAVHEX010000000
VERSION     DAVHEX010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16769
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.050"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            8..1216
                     /locus_tag="VVL45_001608"
     CDS             8..1216
                     /locus_tag="VVL45_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4187763.1"
                     /translation="MNYIKRPHYLDFLRKHRDRPIIKVVSGVRRAGKSVLFQLYKEEL
                     LATGVDEDQIIFINFEDLSYYDLRHFQTLFAYIKDQLVSKKTYYIFLDEIQYVEKFEL
                     VADSLFILANVDLYLTGSNAYFMSSQLATNLTGRYVEIEVLPLSFEEYLSGQSLTENL
                     NTTEIFNNYLFSAFPYLLQTSSYDEKIDYLRGIYNSILLNDIVTRLGKPNPTIIERIV
                     RTLLSSTGSLISTNKIRNTLVSQNVSISHNTLENYLTTLTDSLLFYSVPRFDVKGRAL
                     LQRLEKYYPVDLGLRHLLLPDQKEDIRHILENMVYLELRRRYSQVYVGNLDKYEVDFV
                     VVTDLGHYAYYQVSETTLAPETLERELRPLEAIKDQFPKYLLTMDTIQPTANYNGIEK
                     KSIIDWLLEK"
     gene            1592..1996
                     /locus_tag="VVL45_001609"
     CDS             1592..1996
                     /locus_tag="VVL45_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU4187764.1"
                     /translation="MLFFSISNDLLQLLTSWEKQQRYELAKFGILSNPEQFVFTYIDT
                     KGNINKPLHADYLNNKMKSIRKRHKELTHATPHKLRHTGATLAKKAGMSLEAISEALT
                     HSDTVTTKTYVNTSNIVPLSAGQVAYQHLKNK"
     gene            complement(2259..2651)
                     /gene="rpsI"
                     /locus_tag="VVL45_001610"
     CDS             complement(2259..2651)
                     /gene="rpsI"
                     /locus_tag="VVL45_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEU4187765.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVIGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(2670..3116)
                     /gene="rplM"
                     /locus_tag="VVL45_001611"
     CDS             complement(2670..3116)
                     /gene="rplM"
                     /locus_tag="VVL45_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEU4187766.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            3472..3789
                     /locus_tag="VVL45_001612"
     CDS             3472..3789
                     /locus_tag="VVL45_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEU4187767.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            complement(3939..4751)
                     /gene="folK"
                     /locus_tag="VVL45_001613"
     CDS             complement(3939..4751)
                     /gene="folK"
                     /locus_tag="VVL45_001613"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEU4187768.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFETYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIDKLRARGIHILKESSVLAT
                     EPWGGVEQDSFTNQVVEVETWLPAQVLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(4794..5348)
                     /gene="folE"
                     /locus_tag="VVL45_001614"
     CDS             complement(4794..5348)
                     /gene="folE"
                     /locus_tag="VVL45_001614"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEU4187769.1"
                     /translation="MDTQKIEAAVKMIIEAVGEDANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            complement(5329..6651)
                     /locus_tag="VVL45_001615"
     CDS             complement(5329..6651)
                     /locus_tag="VVL45_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEU4187770.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYTDQISINGESISEARLEALMADY
                     QSLLEGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLFDSTNVCQPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGMPLVTGRIAPEALAVIDRIAEGKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLEQNLTDKKEEKQTIRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            complement(6653..7627)
                     /gene="folP"
                     /locus_tag="VVL45_001616"
     CDS             complement(6653..7627)
                     /gene="folP"
                     /locus_tag="VVL45_001616"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEU4187771.1"
                     /translation="MMKILEGVTDMSSKANHAKTVICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDT
                     WKSQVAEAALAAGANLVNDITGLMGDEKMAHVVAEARAQVVIMFNPVMARPQHPSSLI
                     FPHFGFNQTFTVEELADFETLPIEDLMVAFFERALARAAEAGIAPENILLDPGIGFGL
                     TKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEESGFEVNPETELGFRNRDTAS
                     AHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            complement(7722..8429)
                     /locus_tag="VVL45_001617"
     CDS             complement(7722..8429)
                     /locus_tag="VVL45_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU4187772.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVAGLSIVVLALFIMGARKTKLASFNFSFFRAKDLARLGLSYLVIVGSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            complement(8482..9900)
                     /locus_tag="VVL45_001618"
     CDS             complement(8482..9900)
                     /locus_tag="VVL45_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEU4187773.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHVMIWILDALFILNYISMAL"
     gene            10090..10902
                     /locus_tag="VVL45_001619"
     CDS             10090..10902
                     /locus_tag="VVL45_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU4187774.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKRTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYSLAKEMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            11242..12261
                     /gene="adhP"
                     /locus_tag="VVL45_001620"
     CDS             11242..12261
                     /gene="adhP"
                     /locus_tag="VVL45_001620"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEU4187775.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
     gene            12494..13483
                     /gene="manX"
                     /locus_tag="VVL45_001621"
     CDS             12494..13483
                     /gene="manX"
                     /locus_tag="VVL45_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEU4187776.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            13511..14314
                     /locus_tag="VVL45_001622"
     CDS             13511..14314
                     /locus_tag="VVL45_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEU4187777.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            14338..15249
                     /locus_tag="VVL45_001623"
     CDS             14338..15249
                     /locus_tag="VVL45_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU4187778.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(15305..16639)
                     /gene="pepC"
                     /locus_tag="VVL45_001624"
     CDS             complement(15305..16639)
                     /gene="pepC"
                     /locus_tag="VVL45_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEU4187779.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALERRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQATVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAE"
BASE COUNT         5001 a         3607 c         3269 g         4892 t
ORIGIN      
        1 aactattatg aactatatca aaagaccaca ttatttagat tttttaagaa aacatcgtga
       61 ccgaccaatc atcaaagttg tgagtggagt tagacgagct ggtaaatctg tgctttttca
      121 actctataaa gaggagttac tagcaactgg ggtagacgag gatcagatta tattcatcaa
      181 tttcgaagat ttgagttact atgatctgcg acattttcaa acattattcg cttatataaa
      241 agatcaatta gttagcaaga aaacatacta tatcttttta gatgaaattc aatatgttga
      301 aaaatttgaa ctggtagcag atagtctatt catcttagca aatgtagacc tctatttgac
      361 tggatctaac gcctacttta tgagtagcca attagcaaca aacttgactg gtcggtatgt
      421 tgagatagag gttcttcctt tgtcatttga agaatatcta tcaggtcaat ctctcacaga
      481 gaatctgaat acaacagaaa tttttaacaa ttatctcttt agtgctttcc cttacttatt
      541 gcaaacatca tcttacgatg aaaaaattga ctatctcaga ggaatatata actccatact
      601 gttaaatgat attgtcacta gattgggaaa accaaatcct actattattg agcgcattgt
      661 ccgaaccctt ctcagtagta caggtagctt aatatcaaca aataagattc gcaataccct
      721 agtcagccaa aatgtttcaa tatcccataa tactttggaa aattatttga caactttgac
      781 agatagttta cttttttatt ccgttccacg ttttgatgta aaaggtagag cattattgca
      841 acgtttagaa aaatattatc ccgttgattt aggtttacga catctcttat taccagacca
      901 gaaagaagac attaggcata tcttggaaaa tatggtatat ttggaattga gacgtagata
      961 ttcacaagta tatgttggta atttagataa gtatgaggtt gattttgttg ttgtaactga
     1021 tcttggccac tacgcttatt atcaggtcag tgaaacaaca cttgctccag aaacactaga
     1081 aagagaactt agaccactag aagccattaa agatcaattc cctaaatatc tattaacaat
     1141 ggatacgatt cagccaacag ccaattacaa tggaatcgag aagaaaagca ttatagattg
     1201 gttactagaa aaatagataa atataaatca tacagctaat tagatttgca acagtctgtt
     1261 atcaatgatt ctacccaaat cctaacaaga tatagtgaat ttcgaatacg ctatataata
     1321 cggacacttg aaaatagaaa ttggggatga aaggggatct ataatttctg gaagtactat
     1381 caaaaattaa tatcatagtc ttattagaga atagcatcac ccactttctc aaataagatt
     1441 aaattgtaac tgaattataa tgaaaaagag actgagcaat cagtctttaa aatcagaaaa
     1501 gcgcatagta tcaggtattg aacaaccttg ataatatgcg ttttattatg gaaatatttg
     1561 cttcattttc tcctgaaata gagcttttgc tatcctattt ttctctattt ctaatgattt
     1621 acttcaactt cttacctctt gggaaaaaca acaaagatat gaactagcaa agtttggcat
     1681 cctcagtaat ccagaacaat ttgtttttac atatatcgac acaaaaggaa acatcaacaa
     1741 gcctttacat gccgactatc tgaacaataa aatgaaaagc attagaaagc gacataaaga
     1801 actgacacac gctacaccgc ataaattacg acatacagga gcgacacttg ctaaaaaagc
     1861 aggaatgagc ttagaagcca tttctgaagc attaactcat agcgataccg ttacaactaa
     1921 gacttatgtt aatacttcaa acatagttcc attatctgct ggacaagttg cctaccaaca
     1981 tcttaaaaat aagtagggta attttagggc aaattttagg gtaaactttt ttagaaagta
     2041 tagaaaaaag cacccatagg gtaaaccctt gagtgctttg aaacgttgat ataatcgtat
     2101 tgattaacgt tttgaggcaa ctgaccatgt caggttgcag ccacatttgt aagcgactaa
     2161 agtcgcaaca actgtgtcaa ttgcaccaat ttagtaagaa agtataaaaa aagaacaccc
     2221 cgaaaggtgc tctgtataag tatagtaatt ctttcgaatt aacgtttact aaattgtgat
     2281 gctttacgag ctttcttaag acctggtttc ttacgttcaa ctttacgtga gtcacgtgta
     2341 agaagtcctg cgcgtttcaa tgaatcgcgg aagtctgggt ctacttgaag aagggcacga
     2401 gcgataccgt gacggatagc tcctgattga ccagcgtatc caccacctat aacgttaacg
     2461 aaaacgtcgt atgaacctac agttgaagta actgcgaatg gttggttgat gacaagacga
     2521 aggtcagcgt gtgggatgta ctcttcaaca tcttttttgt taacagtgat tttaccagtt
     2581 cctggaacaa ggcgaacgcg tgcaacagcg tttttacgac gtccagtacc tgcatattgt
     2641 gcttgtgaca tactttattg ttcctttcct tagataagtc ctgaaatgtc aagaacttct
     2701 ggttgttgtg cagcgtgagt gtgctcagct ccaacaaata ctttcaactt cataccttga
     2761 gcgcgtccaa gtgtattgtg tggaagcata cctttaactg atttctcaat caaacgtact
     2821 gcatttttag aacgaagttc acctgcagag atttgtttca atccacctgg gtggtttgag
     2881 tgagtgtagt agattttatc agttgctttt ttaccagtca atttaacttt ttcagcattg
     2941 ataacaatca cgaagtcacc tgtatcagtg tgtggtgtaa atgttggttt gttttttccg
     3001 cgaagtacgc tagcaactac tgcagaaaga cgtccaagtg gtacatcagt tgcgtcaact
     3061 acgtaccatt tacgttcaac ttggcctggt ttagccataa atgttgtttt gttcatgatt
     3121 tctcctatat atagttcgat ttttgtttac ggtgatgggt gttccttccc atcgaaaagt
     3181 atttggaagg ttccggggcc tttcaaatgg ggtaaacaat accgcctact atcataccaa
     3241 attttacccc taaaagtcaa tagatatgaa aaatattttt cggtaacttc tcaaagtaac
     3301 tgaaagttat ataatcaagt taatttggga atttacctat taacttcggt agatttttag
     3361 gaattttttc cctatttctc cctgtaacta aaattctttt caaaaaatca ccttttcttg
     3421 atttttaatc ctatttgctg tatgataagg gaaaagaaag gggacagaga tatggctttt
     3481 accaataccc acatgcgatc tgctagtttt ggtattgtta ccagcttgcc tgatgacatc
     3541 attgactctt tttggtatat catcgaccat ttcttaaaaa atgtctttga attggaagaa
     3601 gaactcgagt ttcaattgct taataaccaa ggaaagatta ccttccactt ttcaagtcaa
     3661 cacctcccta cagccattga ttttgacttt aaccatcctt tcgaccctcg ttatccccca
     3721 agagtactgg ttttagacat ggacggtaga gaaactatcc tcctcccaga agaaaatgac
     3781 ctattttaat actcaatgaa aatcaaagag caaactagga agctagccgc aagctgtact
     3841 tgagtacggt aaggcgacgc tgacgtggtt tgaagagatt ttcgaagagt ataaaaactc
     3901 tagccttcag ttgcaagtaa ctgaaaacta gagtttttct attttttcaa agcatcatac
     3961 aagttgcgga tcggttgttt taatatcgga tggataaaat gaggcgcaat ttcctgtaag
     4021 gactcaagga caaaaaggcg ttccgctatg taaggatgag gcaatatgag gtcgtctgta
     4081 taaaggatct ggtcctccac aaagagcaag tccaaatcaa tcaaacgagg gccccaatgc
     4141 acttctctca cccgtcccag ctctgactca atggctaaca aggtttctaa tagcacttgg
     4201 gcaggcaacc atgtttctac ttcaaccact tgattggtaa agctatcctg ctcaacacca
     4261 ccccaaggct ccgtcgctaa gacactggac tctttgagaa tatggatgcc acgagctcgc
     4321 agtttgtcaa tggcttgctt caagtttgct tgtttatctc ccatattgct tcctagggcg
     4381 ataaaggctc gttgcttgcg gcgatgaatg gttaccgagc aagtatctag tgacaaatgc
     4441 accggtgccc aaggtttttt cagttccaac ttcatttctt ggacaagagg ataggtctca
     4501 aaggtacgtt ccaccagttt ataggctacc gtttcaatca agtcttcact cgtttcctga
     4561 aaccaagtcg tccactgctg acacaattct ccgtaatgga cagaggctgt taaatccaag
     4621 tctgtagctg ccttggtcat atcataggat aggatggctg aaacgacaaa tttctgcccc
     4681 aattctttct cactaggaaa aagaccatga taggcaaaca tttccaaatc cttaatctgc
     4741 agttgatcca taatgattcc tttctagaaa aatccgcttg aagcggattc tttttatagc
     4801 cccattaaac gataagcttg gtcgcggagg tccttatctg tttcaaatag accacgagct
     4861 actgtcgtca aggttgcagt gcctggtttt ctaacaccac gcatactcat acacatatgt
     4921 tccgcctcaa tgacaacaaa ggctccttta gcacctagat agtccatcaa ggcatcggcc
     4981 acttcgatat tcaaacgttc ttgaatttgt ggttttttcg aataaacttc aaccgtacgg
     5041 gctagcttag acaagcccgc cacacgacca tctggaatgt aggcaatgtg cgctctacca
     5101 taaaatggca agaagtggtg ttcacacatg gtatggaaaa agatatcctt ttctaccacc
     5161 atattatcgt caataatttc aaaggatttt gacaaatgtt cctctgctgt ttgaccaaga
     5221 cctgaaaaaa tctcttgata catacgggct acacgagcag gtgtttcctg caagccctcg
     5281 cgattagcgt cctctcctac agcctcgata atcattttta cagctgcttc aatcttttgt
     5341 gtatccattc tcgttcttcc tctccatcag ataggccctc acttggctca agaaatacaa
     5401 ggaacctgtg acaatcctaa ttgtttgttt ctcttctttt ttatctgtca aattctgctc
     5461 tagaaaatca tgccaatctt ggtagctgag atttctagac ttagctgcct ctttcagcac
     5521 gctttcatcc gtcgcccgac tatccgcaaa atgtgttaga gtaagctcgg tatctggcat
     5581 ggctcccagc aagtccaaca tatcctccaa ggccttggtt ttgatacaag tgaagaggat
     5641 ttccttatga taatccgcaa aacgttcttg caaggttacc aacaaggcct tgatagcatg
     5701 gggattgtgg gctccatcca aaatcatcaa gggatctctt gacacgattt ccaaacgccc
     5761 tggccaactt gtttcttcca aggcttgacc aagaaagtca ttgcttgcta gctctcgacc
     5821 atcttcttga caaaaagtat caagtaaagc tatggccatc ccagcattct ctatttggta
     5881 caaaccaagc aggctagtct ggaagcgacc ttgtctgaca gcacttgtat agtcaaagac
     5941 ttcccctgtc accacacttt cttgatgacg aacctgataa tctgtcccgt aggcaagtct
     6001 cggcgcatct ttcccttccg caatgcggtc aatcacagcc aaggcttctg gagcaatacg
     6061 ccctgttacc aagggcatcc cttgtttgat aatacctgcc ttctgctctg ctatggcctc
     6121 caaagtgtca ccaagtaagg ccacatgatc caagccaata gttgtaattc ctgtcaaaat
     6181 gggctgacag acattggtac tatcaaaaag tccacccatg ccaacttcca tgatggccac
     6241 atctacttgc tctgaggcaa agtagtcata ggccagggct gtgataatct caaactcggt
     6301 tgtgccctgt aaattggcga ccgcttctcc ctccagcaaa gactgatagt ctgccatgag
     6361 agcttctagc ctcgcttctg agatcgattc cccattgatg ctaatctggt ctgtgtaatg
     6421 aatgagatag ggcgagctaa acacgccaac tctcagccct agcttttcta gcatcttttt
     6481 caaaaaagca atagtcgagc ccttgccgtt agttcctccg atatggagga ccttgagttt
     6541 gagatgggga ttgccacgca aagctaacag ttccaccatt cgttccaagc caaaatgcgg
     6601 ttgatccgtc cggtagttag caatccactg attgttttca atttctttca tcttatttat
     6661 attgttttaa atctaaattt tccgcttcat cagccagacg aatggcagag gcaatttcaa
     6721 ctgccatcct gtgactagct acgtcatgca cgcgcaccac ttctacaccc tgtctcgcag
     6781 cgatactagt tacatgagcc gaagccgtgt cccgatttcg gaaaccaagc tctgtctcag
     6841 gattgacttc aaacccactt tcttcaagga tattgatgac aaatcgcttg cgcgacactc
     6901 cgagaaagat tggatagccc ttctgatgta gtttatccag atcccgtaaa aggaggagat
     6961 tttctttctt ggtcagacca aagccaattc ctggatccaa caggatattt tctggtgcaa
     7021 taccagcttc tgccgctctc gctagtgctc gttcaaagaa agccaccatc aagtcttcga
     7081 ttggcaatgt ttcaaagtca gctaactctt ctactgtaaa agtttgatta aatccaaaat
     7141 gagggaatat gagcgagcta gggtgttgag gtcgcgccat aactggatta aacatgatga
     7201 ccacttgcgc tctcgcttca gctaccacat gagccatttt ttcatcgccc atgagaccag
     7261 tgatatcatt aactagattg gcaccagcag ccaaagcagc ctctgctact tgactcttcc
     7321 aagtatcaat agagatgagg acatcacttt ccttgcgaat cgctttgatc actggaacaa
     7381 cacgctggat ttcctcttct atctcaacat agctacttcc cggccgagtc gattctccgc
     7441 cgatatctag catactggct ccttctgcta tcaatttacg agcctgctgg agcgcctgct
     7501 caagagcaaa aaattgacca ccgtccgaaa aggagtctgg ggttacattg ataattccgc
     7561 aaataactgt ctttgcatga ttggctttac ttgacatatc ggtcactccc tcaaggattt
     7621 tcatcatatt atttttctat tttaccataa aaagaaaaag atggacacga ttgcattcat
     7681 ctttccctgg tagaaagagg tcggttccta cataaaaact tttaaacaga aattcctaat
     7741 gtccgactca taatcaccac aagagccaac aaacagaaag caatcccatt aacaatcatg
     7801 tgaagcaaga tcgacatttc caaacgttgg gtcttgtagg ctgtccaaga tagaactgtc
     7861 gacatacctc cataaatcaa taaagaaggt aaattacttg gttgatgcaa taaagcaaac
     7921 acaatcgtac cgactacaaa tcccaagttc tccttgcctc ggaaaatctt tttaggaaca
     7981 atcccacgac acaagatttc ctcacaaatc ggagcaagca aggctagcaa gaagaaactg
     8041 gaaatcaacg aactattttg aaccatatca ttaatctgag actggttagc tgttgtcgtc
     8101 tcatttgaca gttgcaataa aatggaacca agtatatttg acccgacaat aactagataa
     8161 ctcaagccca aacgtgccaa atctttagct ctaaaaaaag aaaaattaaa actagctaac
     8221 ttggttttac gagctcccat aataaataga gccagaacca caattgaaag gccagcaact
     8281 atcagccctg actgtagcag ggctacttct tttaaagtca aaatagaggt aaccactagg
     8341 ggaacctgat ataaaaggac ggcaagtaaa aagaccaaaa tccagcctgc acgattcaac
     8401 aattctttcc acatattttt ctctttcatc acctatctcc tttgtattaa aaaagggggg
     8461 aaatccccct gggtcattct attataaggc catgctgatg tagttaagga taaacaaggc
     8521 atccaaaatc caaatcatga catgaacatc tttaacttga cctttaacaa gcttagtcaa
     8581 agtgtaagtc aagaaaccaa ctgcaatccc ttgagtgata gagtagctga atcccataaa
     8641 gatagatgtg aagaaggcag gaactgcttc agacatatca tcccaatgga tatttttcaa
     8701 gctaccaagc atcataatcc caacgataat caagattgga gctgtagccg ctgttggtac
     8761 gatcgctaga agtgggctaa agaagcttga aatcgcaaaa cagatagcta caaccaaggc
     8821 tgtcaaacca gtacgtccac ctgcaccgat accagcagca gactcaacat aagtcgttac
     8881 gtttgaagta cctgcaatgg caccgactgt cgttccaatc aaatcagagt aaagagcctt
     8941 atccaatttg gctgattggt gattttcacc atttgtcgct acgataccaa ctttttcacc
     9001 tgtaccgatc aaggtaccaa ttgtgtcaaa aatatctgtc aatgagaagg caagaatggc
     9061 catcagagtt tcaggcaagc gagctgtatc tgaaaccaaa gctcccaatc cttctgaacc
     9121 aagagctgca ccaaagattg tcttcaaatc ttcaaaagct gcaccaacat ggttattagc
     9181 aaaatcgata ctagacaaat caaccaaacc aactgcaata gcaagaacag ttgttgtcaa
     9241 gatagagaga ataattcccc ctttaatccc tttgatgaca aagaagatag taatggcaag
     9301 tcctgcaaga gccactaaaa cagctggatt attaaagctg accaatcctg gaactgctga
     9361 agagtttgct gcaatcgttg cttgagcttt gtcagcccct tctcctacaa cagtatagtt
     9421 gcctggatca atcgtgaatt tcaaaagtcc agcattctta atccctacat aggcaaggaa
     9481 gacaccgata ccagctgaaa tagctgagcg aagagcattg ggaatcgatt caatgatcat
     9541 tttacgaaca tttgtcaagg taataatcaa tgaaataatc ccacagatga agaccatagc
     9601 tagggcttct tgccaagaat aaccaagccc gaatacaact gtaaaggtaa agaaggcatt
     9661 gagtcccata cctggcgctt gggcataagg taagttagca taaaaagcca tcatcaaggt
     9721 acccgctact gcaccaataa tcgtcgctag gaagacgccc tgagcaggca ttcctgtttg
     9781 tgaaagtatt tgtgggttta caaagagaat atagctcatt gcaaagaaag ttgttaaacc
     9841 agcgagaacc tctgtacgaa cgtctgtacc gttctctttt agtttaaata atttgtccat
     9901 catcaatctc cttttaattt ttacgaacaa taatataatt atatcattta tcattcactt
     9961 tttcaatgtc tttgtttgat ttgtttaaga aattaatcca atttcatttt ttgtttggaa
    10021 tctgcttttc tgcctgcttt ctgttataat agaagacatc ttatctgaaa acacactaga
    10081 aaggtccata tgacgaaaaa aattattgca gttgacctgg atggaaccct gctcaactca
    10141 gacagtcaaa tttctgactt taccaaaaga accatcaaaa aagttgctga aaaaggccat
    10201 caggttatta ttacgacagg tcgcccttac cgtatgtcaa aagattttta ccgtgaactg
    10261 ggcttagaca ctcctatgat taacttcaac ggatccctta ctcatttacc agaccaagtt
    10321 tgggattttg aaaagtgttt gactgtagac aaaaaatatc tgctagatat ggttcaacgt
    10381 tcagaggaca ttcaagccga ttttatcgct ggagaatatc gtaaaaaatt ctacattaca
    10441 aatcccaatg aagaaattgc caatcccaaa ctatttggtg tagaagcttt ccagcctgaa
    10501 gatcaattcc agcctgaatt ggtgaccaag gaccctaact gtatcctctt gcagactaga
    10561 gccagtgaca aatattcctt ggcaaaagaa atgaacgcct tctaccagca tcaactttct
    10621 atcaatacct ggggaggtcc gctcaatatc cttgaatgta ccccaaaagg tgtcaacaag
    10681 gcctttgctt tggactactt gctcaagata atgaatcgtg acaaaaaaga tttgattgcc
    10741 tttggagatg aacacaatga taccgaaatg ctcgcttttg ctgggaaggg ttatgccatg
    10801 aaaaatgcca atccagagct actcccttat gcagatgagc aaatttccct taccaacgac
    10861 caagatgggg ttgccaaaac cctacaagac ttattcttat aacctatact gatactcaat
    10921 gagaatcaaa gagcaaactt aggaagctag ccgtaggttg ctcaaagcac tgctttgagg
    10981 ttgtagatga agctgacgtg gtttgaagag attttcgaag agtatgacca gcttgcaagc
    11041 tggtctttgt gctcttttca cagtctcatc aaccagttaa tcgattgttc tactttttgt
    11101 ctccaaaacc ttggaaaagg ctttcttttg tgatatactt cacatataaa tacaagagag
    11161 ctcgtcacac tcgactctgt tgacaaaaaa tcaatccagt cccgttgtcc ctgttctcgg
    11221 tcaatatcaa ggaggatagc tatgaaagct gttgttgtaa atccagaaag cactggtgtt
    11281 gctattgaag aaaaagtact ccgtccactt gaaactgggg aagcacttgt agaagttgaa
    11341 tactgtggcg tttgccacac cgacctccac gttgctcatg gtgactttgg tcaagtccca
    11401 ggacgtgttc ttgggcacga aggtatcggt atcgttaaag agattgctcc agatgtgaaa
    11461 agccttaaag tcggtgaccg cgtcagcgtt gcttggttct ttgaaggatg tggcacttgc
    11521 gaatactgta caactggtcg cgaaaccctt tgccgtacag tgaaaaatgc tggctactca
    11581 gtagacggtg gtatggctga acaatgtatc gtaactgctg actatgctgt caaagttcct
    11641 gacggacttg atccagccca agcttcttct atcacatgtg ctggagtaac aacctataaa
    11701 gctatcaaag aagcaaaagt tgaaccaggc caatgggttg ttctttacgg tgctggtgga
    11761 cttggtaacc tcgctgttca atacgctaaa aaagtattca atgctcatgt tatcgcagtc
    11821 gatatcaaca atgacaaact tgcccttgca aaagaagtag gcgctgacat tgtgattaac
    11881 ggcctcgaag ttgaagatgt agctggactc attaaagaaa aaactgatgg aggagctcat
    11941 tcagctgtcg taactgctgt gtctaaagtt gccttcaacc aggctgttga ctccattcgt
    12001 gctggtggtc gcgtcgtcgc tgttggtctt ccttctgaaa tgatggaact cagcatcgtt
    12061 aaaacagtcc tcgatggaat ccaagtcatc ggttctcttg tcggaactcg taaagactta
    12121 gaagaagcct tccaatttgg tgcagaaggt ctggtagtcc cagttgttca aaaacgtcca
    12181 gtagaagatg ctgttgccat tttcgacgaa atggaaaaag gccaaatcca aggacgtatg
    12241 gtactcgact tcacccacta atctaataga aacctatttt aaaagttgga agcttcttcc
    12301 aactttttta tattaaattt taaataagaa ttcaagatta ctcctcaaaa actcaacata
    12361 tcaacataca aaaataaaaa ttttttaatt ttttatgata aatagttgct ttttttatga
    12421 aaacggttta tacttaaaca gtcttaaagt tttcttaaat gaaaactaaa acaaaaaagg
    12481 aggaatgaca ataatgagta tcggaatcat tattgcgagc cacggcgaat ttgctgcggg
    12541 tattcatcag tcaggatcta tgatctttgg tgaacaagaa aaggttcaag ttgtgacctt
    12601 tatgccaaat gaaggtcctg atgatctata cgctaagttt aataacgctg ttgctgcatt
    12661 tgacgcagaa gatgaggttc tagttttggc tgacctttgg agtggttctc catttaacca
    12721 agctagtcgc gtgatgggag aaaatcctga gcgtaagttt gccatcatca caggacttaa
    12781 cttaccgatg ttgattcaag cctacacaga gcgcctcatg gacgctgctg caggtgtaga
    12841 aaaagtcgct gctaatatca ttaaagaagc caaagatggc atcaaagctc ttccagaaga
    12901 gctaaatcca gtcgaagaag ttgcaagcgc tgcagctgct ccagttgccc aaactgctat
    12961 cccagaagga actgttatcg gagacggtaa attgaaaatc aatcttgccc gtcttgacac
    13021 acgtctactt cacggtcagg ttgcaactgc ttggactcca gattcaaaag caaatcgtat
    13081 catcgttgct tcagataacg tggctaaaga cgaccttcgt aaagaattga ttaaacaagc
    13141 agctccaggt aatgtcaagg ctaacgtggt tccaattcaa aaactgattg agatttcaaa
    13201 agacccacgt tttggagaaa cacatgccct tatcttgttt gaaacacctc aagatgccct
    13261 tcgtgccatc gaaggcggcg tgccaatcaa gactcttaat gttggttcta tggctcactc
    13321 aacaggtaaa acattggtca ataccgtttt gtctatggac aaagaagacg ttgctacatt
    13381 tgaaaaaatg cgtgacttgg gtgttgaatt tgatgtccgt aaagtaccaa atgattctaa
    13441 aaaagatttg tttgacttga ttaacaaagc caatgtcaaa taagccatta tttatgaaag
    13501 gattttaaac atgtctatta tttctatggt tttagtagtc gttgtagcct tctttgcagg
    13561 tcttgaaggc atcctcgacc agttccaatt tcaccaacca cttgtagcct gtacccttat
    13621 tgggcttgta acaggtcact tggaagcagg gattatcctc ggtggatcgc ttcaaatgat
    13681 tgcccttggt tggtcaaata tcggtgctgc tatcgctcct gatgctgcac ttgcttctgt
    13741 cgctgctgcc attatcatgg ttcttggtgg tgactttacc aagactggta tcggtgttgc
    13801 ccaagcggtt gctatccctc ttgctgtagc tggacttttc ttgacaatga ttgttcgtac
    13861 aatttcagtt ggtttggttc atactgcaga tgctgccgct aaaaaaggtg acttcggcgc
    13921 tgtggagcgt gcgcatttca tcgcgctact tttccaagga cttcgtatcg cgcttcctgc
    13981 agctcttctc cttatggtac caactgaaac tgtacaaagt atccttagtg ccatgccaga
    14041 ctggctcaaa gatggtatgg ctatcggtgg tggtatggtc gttgccgttg gttacgccat
    14101 ggttatcaac atgatggcaa ctcgtgaagt atggccattc ttcgctcttg gtttcgttct
    14161 cgctgctgtg tcagatatta ctctaatcgg attcggtgct atcggcgttg ctatcgctct
    14221 tatctacctt cacctttcta aaactggtgg aaatggtggc ggaggagccg caacttctaa
    14281 cgacccaatc ggcgatatcc tagaagacta ctaagataag aaaggactga aaacatcatg
    14341 actgaaaaac ttcaattaac taaatcagat cgtaaaaaag tttggtggcg ttcaaccttc
    14401 ttacaagggt cttggaactt tgaacggatg caaaacttgg gctgggctta tacactcatt
    14461 ccagctatca aaaaactcta tactaaaaaa gaagatcaaa tcgctgctct tgagcgtcac
    14521 cttgagttct tcaacactca tccatacgta gctgctccag tcatgggggt tactcttgcg
    14581 cttgaagaag aacgtgctaa cggtgtggaa atcgatgacg ctgctatcca aggggttaaa
    14641 atcggtatga tgggacctct tgctggtatc ggtgacccag tattctggtt tacagtacgc
    14701 ccaatccttg gatctctcgg tgcttcactt gcccttactg gcaatatctt ggggccactc
    14761 ctcttctttg ttgcatggaa cttgattcgt atgtcattct tgtggtatgt tcaagagatt
    14821 ggatacaagg ctggatcaga aatcactaaa gatatgtctg gtggtatcct tcaagatatc
    14881 actaaaggag cttctatcct tgggatgttc attcttgctg tccttgttca acgctgggta
    14941 aatattaaat ttgctttcga tgtttctaaa gttcaactag atgaaaaggc ttatatccat
    15001 tgggataaat tgccagaagg gtctaaaggt atccaagaag cattcgcaca agtaggacaa
    15061 ggattgtctc aaactcctga aaaagttact actttccaac aaaacttgga tatgttgatt
    15121 cctggattat caggactact ccttacttta ctttgcatgt acttacttaa gaaaaaagta
    15181 tctccaatca ctattatcct tgccctcttc gcagtgggta ttgtggcaca tgttcttcac
    15241 atcatgtaat caagcaacta aaaaggaacc aggttctaaa atctgattcc ttttttctat
    15301 gcttttattc agccaaggct cccattggat cccatggtgc aagtacgatt ggttctgctc
    15361 cataggcagc ttgttcttct gctgtcagca attccttacg aacaacgatt tggtatgtgt
    15421 attcgtccat ccaagcgtct gaggcaacaa agtaaccatc tgtaccgacc ttgtctcccc
    15481 atgagttttc aaccttccac ttggttgatt taccattttc gtccaagtca acacctgtca
    15541 agaccatggc gtgggtcatc aagctttcac tatagtccaa acgtccagcc ttgtcttgag
    15601 taagtttaat gtccatgctt gattcaaagt cataaacatc tgtcgcaagg atgccagcct
    15661 tacggttgct gagctggccg acatcagaac caaaccaaac agtctcacct gcttgcattt
    15721 gggcaatcgc caattctttc aagcgctcca ttggaacgtt gatgtagcga actgcacggc
    15781 taccaaccac attccccaac atctcaactg tgtaagattt tccgtaaggt ttatcagcag
    15841 ttggagcatt gataacagaa acgtagtctt ctaaaggaag attgacatat ttcttgtaaa
    15901 actcttgtgg tgtgattcct ttttcacttt tgtagttgtt atctttatcg cgataagcaa
    15961 agtcaaactt gcgtggtgga agtcctaatg acatagcaag aaagttaaag atttcttgca
    16021 agaggtcttc tttcttagct tgaacagtcg cttgatctgc accagaaaca agcaagtcac
    16081 gcaagatttg agcatcttga cgaagcaatt tattaaggat cgcatttagc tcacgactgc
    16141 tgctagatga aacagactca ggataaactg acttaggcac gacaccgtat ttttcaaaga
    16201 gggaaacgac catatcccat tgaccgccat cttgttgagg tgtttggagt aagaagctaa
    16261 ccttgcggct agtcaattct tggtctgaag tcgcaatgac ttgctccaag aaccagtttg
    16321 atttctcata cttatcccag aagaaagtgt gggcttgtga caactcaaag ttctccaatt
    16381 tgtattgcga gatgagtttg tggcggaagg tgttgagagc cgcaaacatc cagcaacgac
    16441 cagacgcttt ctggttagtg accttgtcct tggttaaatc caatgagaaa acaggtgtgt
    16501 tgtctacatg gctttggcga cgttccagag ctgcaaaaat tccgttgtgg ctggcagcat
    16561 tttcaatcgc ttggtatttt acatttgctt catagttggc aaatagttta tcagtaaatg
    16621 attcttgaat cgcgttcata gattcctcct tttattctac agtctattat aactcttttc
    16681 acaaaggaag caactaaaaa acataaaagg cgaggacatt gtccctcacc tttttcgaat
    16741 ttatactctt cgaaaatcaa attcaaacc
//
LOCUS       DAVHEX010000051        14599 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVHEX010000051 DAVHEX010000000
VERSION     DAVHEX010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14599
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.051"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            104..844
                     /locus_tag="VVL45_001625"
     CDS             104..844
                     /locus_tag="VVL45_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU4187780.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            841..1752
                     /gene="pfkB"
                     /locus_tag="VVL45_001626"
     CDS             841..1752
                     /gene="pfkB"
                     /locus_tag="VVL45_001626"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEU4187781.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVESVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDVVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            1749..3701
                     /locus_tag="VVL45_001627"
     CDS             1749..3701
                     /locus_tag="VVL45_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU4187782.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTDHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGALYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            3865..6090
                     /locus_tag="VVL45_001628"
     CDS             3865..6090
                     /locus_tag="VVL45_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEU4187783.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKTQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEIAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(6135..6563)
                     /locus_tag="VVL45_001629"
     CDS             complement(6135..6563)
                     /locus_tag="VVL45_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187784.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTVATIASILGLITASITIMN
                     ALDNNKNIKKEPTTSQTTTIVKEIQKESPKENTSPTKETNTSQEKTQQEETPKSSVKE
                     EKKEDQKTATQDSSTPASSKPATENEKQSNAPTSENKSNQ"
     gene            6701..7843
                     /locus_tag="VVL45_001630"
     CDS             6701..7843
                     /locus_tag="VVL45_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU4187785.1"
                     /translation="MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWLIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKSQGFEVDFAPVDKKGLVDVEALAGLIRHDTILVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     NYPDIFVFSDEENFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAKSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            7852..9066
                     /gene="thiI"
                     /locus_tag="VVL45_001631"
     CDS             7852..9066
                     /gene="thiI"
                     /locus_tag="VVL45_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEU4187786.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYTQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSSVQEIMRDIYKE
                     GMTFKISSKRSDHNFELDSRELNQTLGGAVFEAIPNVQVQMKSPDINLQVEIREEAAY
                     LSYETIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            9201..10025
                     /locus_tag="VVL45_001632"
     CDS             9201..10025
                     /locus_tag="VVL45_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU4187787.1"
                     /translation="MNQSYFYLKMKEHKLKVPYTGKERRVRILLPKDYEKDTDRSYPV
                     VYFHDGQNVFNSKESFIGHSWKIIPAIKRNPDISRMIVVAIDNDGMGRMNEYAAWKFQ
                     ESPIPGQQFGGKGVEYAEFVMEVVKPFIDETYRTKADCQHTAMIGSSLGGNITQFIGL
                     EYQDQIGCLGVFSSANWLHQEAFNRYFECQKLSPDQRIFIYVGTEEADDTDKTLMDGN
                     IKQAYIDSSLCYYHDLIAGGVHLDNLVLKVQSGAIHSEIPWSENLPDCLRFFAEKW"
     gene            10047..10786
                     /locus_tag="VVL45_001633"
                     /pseudo
     CDS             10047..10786
                     /locus_tag="VVL45_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            10799..11965
                     /locus_tag="VVL45_001634"
     CDS             10799..11965
                     /locus_tag="VVL45_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU4187788.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIGRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMEQMIADFGQRQE"
     gene            12473..13966
                     /locus_tag="VVL45_001635"
     CDS             12473..13966
                     /locus_tag="VVL45_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEU4187789.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            13979..>14599
                     /locus_tag="VVL45_001636"
     CDS             13979..>14599
                     /locus_tag="VVL45_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU4187790.1"
                     /translation="MKKVKLGEVLSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQYLREHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKGLITKRK
                     LQLDELNLLVKSRFNEMFGDPLNNNKKFAVKTGQQCFKFSSGKFLDK"
BASE COUNT         4378 a         2713 c         3275 g         4233 t
ORIGIN      
        1 taagaaaaaa tgatagattt tgaaagaaaa tgataaaaaa tgcttgactt cgaaagaaag
       61 tatgatagaa tgaatagtgt aaacgataac aggaggtgat tcagtgttaa aaacagaacg
      121 taaacaacta attttagagg agttaaatca acatcatgta gtttctctag aaaaattagt
      181 tagtttgcta gaaacgtcag aatcaacggt tcgaagagac ttggatgagt tggaagcgga
      241 aaacaagctt cgtcgtgtgc atggtggagc agaactcccc cactccttac aggaagaaga
      301 aaccattcaa gaaaaatctg tcaaaaacct tcaagaaaag aagttgctgg ctcagaaagc
      361 agcctctctc attaaagaaa aagatgtcat ctttatcgat gctggaacaa caactgcttt
      421 tttgattcat gaattggtca ataagaatgt tacagttgtg accaactcca ttcaccatgc
      481 cgctcagttg gttgaaaagc agattccaac tgtcatggtt ggaggaaacg tcaagacggc
      541 gacagatgct agtatcgggg gcgttgctct taaccagatt aaccaattgc actttgaccg
      601 tgcctttatc ggaatgaatg gtgttgacga tggctattat acgactcctg atatggagga
      661 aggagctgtg aaaagagcta ttttggagaa tgccaagcag acctacgtct tggtggattc
      721 gtcaaaaatt ggacaaactt gctttgccaa ggtagcccca ctcaaacgcg ctatcgttat
      781 cactagtcaa gggcatgagc tcttgcaggt tattaaggag aaaacggagg taatagaagt
      841 atgatttata cagtcacact caatccatcc attgactata tcgttcgttt ggaccaagtc
      901 aaagttggta gtgtcaatcg tatggacagt gatgataagt ttgctggtgg gaaaggaatc
      961 aatgtcagcc gtgtcttgaa acgtttgaat ataccaaata cagcgacggg atttatcggt
     1021 ggctttactg gtaaatttat cacagatact ttagcagagg aagaaatcga gacacgtttt
     1081 gtccaggtgg cagaagatac tcgtatcaat gttaaaatca aagcagacca agaaacagaa
     1141 atcaacggaa cgggtccaac tgttgaatcg gttcagctag aagaattgaa agctatttta
     1201 tctagtctga cagcagaaga tacagttgtc tttgcaggtt caagtgctaa aaatctaggc
     1261 aatgttatct ataaggattt gatttccttg acgcgccaga ctggtgcgca agtggtctgt
     1321 gactttgaag gacagacctt aattgatagt ttggactacc agcctcttct tgtaaaacca
     1381 aacaatcatg aacttggagc gatttttggg gttaaactcg aaagtttaga tgaaattgag
     1441 aaatacgctc gtgagttact ggctaagggt gctcaaaatg ttattatctc tatggctggt
     1501 gatggtgccc ttcttgtcac atctgaggga gcttacttcg ctaaaccaat caaaggaaca
     1561 gtcaaaaatt cagttggagc tggtgattct atggttgctg gattcacagg tgaatttgtc
     1621 aaatcaaaag acgtagtaga agccttcaaa tggggagtgg cttgcggaac ggcaactacc
     1681 ttctcagatg acttggcaac ggcggaattt attaaagaaa catatggaaa agttgaggta
     1741 gaaaaacgat gaaaattcaa gacctattga gaaaagatgt catgttgcta gatttgcagg
     1801 caactgaaaa aacagctgtc atcgacgaga tgattaaaaa tttgacagac cacggttatg
     1861 taacagattt tgaaacattt aaagaaggaa ttttggcgcg tgaagctttg acttctactg
     1921 gtttgggtga tggaatcgca atgcctcaca gcaaaaacgc tgctgtcaaa gaagcgacag
     1981 ttctatttgc taagtcaaat aagggtgttg actacgagag cttggatgga caagcaactg
     2041 acctcttctt catgattgca gctccagaag gtgccaatga tactcacttg gcagccttgg
     2101 cagaattgtc tcaatacttg atgaaagacg gttttgcaga caaacttcgt caagcaacat
     2161 ctgcagacca agttatcgaa ctttttgacc aagcttcaga aaaaactgag gaacttgttc
     2221 aagcacctgc taatgactct ggtgacttta tcgtagctgt tacagcttgt acaacaggta
     2281 ttgcccacac ttacatggcc caagaagccc ttcaaaaagt agctgctgaa atgggggttg
     2341 gtatcaaggt cgaaaccaac ggtgctagcg gtgttggaaa tcaactaact gcagaagata
     2401 tccgtaaggc taaagctatt atcattgcag cagacaaggc cgttgaaatg gatcgatttg
     2461 atggaaaacc attgatcaat cgtccagttg ctgacggtat ccgtaagaca gaagagctaa
     2521 ttaacttggc tctttcagga gatactgaag tctaccgtgc cgctaatggt gccaaagctg
     2581 caacagcctc taacgaaaaa caaagccttg gtggtgcctt gtacaaacac ttgatgagtg
     2641 gtgtatctca aatgttacca ttcgttatcg gtggtggtat catgattgcc cttgccttct
     2701 tgattgacgg tgctttgggt gttccaaatg aaaaccttgg caatcttggt tcttaccatg
     2761 agttagcttc tatgttcatg aaaattggtg gagctgcctt tggtttgatg cttccagtct
     2821 ttgcgggtta tgttgcctac tctattgctg aaaaaccggg tttggtagca ggtttcgtgg
     2881 ctggtgctat tgccaaagaa ggttttgcct ttggtaaaat tccttatgcc gcaggtggtg
     2941 aagcaacttc aactcttgca ggtgtctcat ctggtttcct aggtgccctt gttggtggat
     3001 ttatcgcagg tgccttggtt cttgccatca agaaatacgt taaagttcct cgttcactcg
     3061 aaggtgctaa atcaatcctt ctattgccac ttcttggaac aatcttgaca ggatttgtta
     3121 tgctagctgt gaatatccca atggctgcaa tcaacactgc tatgaatgac ttcctaggcg
     3181 gtcttggagg aggttcagct gtccttcttg gtatcgtcct tggtggaatg atggctgttg
     3241 acatgggtgg accagttaat aaagcagctt atgtctttgg tacaggtacg cttgcagcaa
     3301 ctgtttcttc aggtggttct gtagccatgg cagcagttat ggctggagga atggtgccac
     3361 cacttgcaat ctttgtcgca actcttcttt tcaaagataa atttactaag gaagaacgta
     3421 actctggttt gacaaacatc atcatgggct tgtcatttat cactgaggga gcgattccat
     3481 ttggtgccgc tgacccagct cgtgcgattc caagcttcat ccttggttca gcagtagcag
     3541 gtggactcgt tggtcttact ggtatcaaac tcatggcgcc acacggagga atcttcgtta
     3601 tcgcccttac ttcaaatgct ctcctttacc tcgtttctgt cttggtagga gcaatcgtaa
     3661 gtggtgtggt ttatggttac ctacgcaaac cacaagcata aaaaatagaa aaatgaaaag
     3721 attggaccgt ttggtgcagt ctttttctct tcccgaaatg cctgtgaaat atggtataat
     3781 agaagaatgg caaacaagaa tacaagtaca acaagacgga gaccgtctaa agcagaactg
     3841 gaaagaaaag aagcgattca acgaatgttg atttcgttag gaattgcgat tttattgatt
     3901 ttcgcagcct tcaaattagg ggctgcaggt ataacccttt ataatttaat tcgcttgcta
     3961 gtgggtagcc tagcttatct ggcgatattc ggcctattaa tctatctctt ctttttcaag
     4021 tggatacgaa aacaggaagg actcttatct ggctttttca ccatatttgc tggcttactc
     4081 ttgatttttg aggcctactt ggtttggaaa tatggtttgg acaagtccgt tctaaaaggg
     4141 accatggctc aggttgtgac agatctgact ggttttcgaa cgactagctt tgctggaggg
     4201 ggcttgatcg gggtcgctct ttatattcca acagcctttc tcttttcaaa tatcggaact
     4261 tactttattg gttctatctt gattttagtg ggttctctcc tagtcagccc ttggtctgtt
     4321 tacgatattg ctgaattttt cagtagaggc tttgccaaat ggtgggaagg gcacgagcgt
     4381 cgaaaagagg aacgctttgt caaacaagaa gaaaaagctc gccaaaaggc tgagaaagag
     4441 gctagattag aacaagaaga gactgaaaaa gccttactcg atttgcctcc tgttgatatg
     4501 gaaacgggtg aaattctgac agaggaagct gttcaaaatc ttccacctat tccagaagaa
     4561 aagtgggtgg aaccagaaat catcctgcct caagctgaac ttaaattccc tgaacaggaa
     4621 gatgactcag atgacgaaga tgttcaggtc gatttttcag ccaaagaagc ccttgaatac
     4681 aaacttccaa gcttacaact ctttgcacca gataaaccaa aagatcagtc taaagagaag
     4741 aaaattgtca gagaaaatat caaaatctta gaagcaacct ttgctagctt tggtattaag
     4801 gtaacagttg aacgggccga aattgggcca tcagtgacca agtatgaagt caagccggct
     4861 gttggtgtaa gggtcaaccg catttccaat ctatcagatg acctcgctct agccttggct
     4921 gccaaagatg tccggattga agcaccaatc cctgggaaat ccctaatcgg aattgaagtg
     4981 cccaactccg atattgccac tgtatctttc cgagaactat gggaacaatc gcaaacgaaa
     5041 gcagaaaatt tcttggaaat tcctttaggg aaggctgtta atggaaccgc aagagctttt
     5101 gacctttcta aaatgcccca cttgctagtt gcaggttcaa cgggttcagg gaagtcagta
     5161 gcagttaacg gcattattgc tagcattctc atgaaggcga gaccagatca agttaaattt
     5221 atgatggtcg atcccaagat ggttgagtta tctgtttaca atgatattcc ccacctcttg
     5281 attccagtcg tgaccaatcc acgcaaagcc agcaaggctc tgcaaaaggt tgtggatgaa
     5341 atggaaaacc gttatgaact ctttgccaag gtgggagttc ggaatattgc aggttttaat
     5401 gccaaggtag aagagttcaa ttcccagtct gagtacaagc aaattccgct accattcatt
     5461 gtcgtgattg tggatgagtt ggctgacctc atgatggtgg ccagcaagga agtggaagat
     5521 gctatcatcc gtcttgggca gaaggcgcgt gctgcaggta tccacatgat tcttgcaact
     5581 cagcgtccat ctgttgatgt catctctggt ttgattaagg ccaatgttcc atctcgtgta
     5641 gcatttgcgg tttcatcagg aacagactcc cgtacgattt tggatgaaaa tggagcagaa
     5701 aaacttcttg gtcgaggaga catgctcttt aaaccgattg atgaaaatca tccagttcgt
     5761 ctccaaggct cctttatctc ggatgacgat gttgagcgca ttgtgaactt catcaagact
     5821 caggcagatg cagactacga tgagagtttt gatccaggtg aggtttctga aaatgaagga
     5881 gaattttcgg atggagatgc tggtggtgat ccgctttttg aagaagctaa gtctttggtt
     5941 atcgaaacac agaaagccag tgcgtctatg attcagcgtc gtttatcagt tggatttaac
     6001 cgtgcgaccc gtctcatgga agaactggag atagcaggtg tcatcggtcc agctgaaggt
     6061 accaaacctc gaaaagtgtt acaacaataa aaaaatagct tctttccaag tttggaggga
     6121 agctatttta gtggctattg attgctttta ttttctgaag ttggcgcatt ggactgtttt
     6181 tcgttttcag tagcaggttt acttgaagca ggagtagaag agtcctgagt tgctgttttc
     6241 tgatcttctt ttttctcttc cttgacgcta gattttggtg tttcctcttg ctgtgttttt
     6301 tcttgactag tgttagtctc tttagttgga ctggtgtttt ccttagggga ttccttttgg
     6361 atttctttga caatggttgt cgtctggctt gtcgtaggtt cttttttaat atttttgtta
     6421 ttatccaagg cgttcataat cgtgatactt gcggtaatca agccaaggat actagcgatt
     6481 gtagcaacgg ttttttgaaa gactgtaaag ccttttttga tgtgttctgt ttttggtttt
     6541 gaagttctac gatagttaga catacattct ctcctttgat tatgatttat tatactctgt
     6601 ttctttaaaa aaatcttaaa aaaagagaaa tttccctttc ttgtcgcaag ctccgtttca
     6661 tgatacaata gaggaagtga ttttagaaag cgtgagaaac atgatttact ttgataattc
     6721 agcgacgacc aagccttatc ctgaagccct tgaaacctat atgcaggtcg cttcaaaaat
     6781 tttaggaaac ccatctagtc tccatcgttt gggagaccaa gcaacacgaa tcttagatgc
     6841 ttctcgccaa cagattgcag atttaatcgg taagaaaagc gatgaaatct tctttacctc
     6901 gggtggaaca gaaggggata actggcttat caagggtgtg gcctttgaaa aagctcagtt
     6961 tggcaagcac atcattgttt cagccattga acatccagca gtcaaagagt cagccctctg
     7021 gttgaaaagt caaggatttg aagtggattt tgctccagtt gataagaaag gcttggtcga
     7081 tgttgaggcg ttagcaggtt tgatacggca tgatacaatc ctcgtttcca tcatggctgt
     7141 gaacaatgaa atcggctcta tccaacctat tgaggctatt tcagaattct tggcagacaa
     7201 gccgactatt tccttccacg ttgatgcggt tcaggcgctt gccaaaattc cgactgaaaa
     7261 gtatctgaca gaacgggtgg attgcgcgac tttctctagt cacaagttcc acggggttcg
     7321 aggtgttggc tttgtctata tcaaatctgg caagaagatt acacctcttc ttacaggtgg
     7381 tggccaggag cgagattatc gttcgacaac tgaaaatgtg gcagggattg cagcgacagc
     7441 caaggccctc cgtttgtcta tggaaaagct agatatcttt aggagcaaga ctgggcagat
     7501 gaaggcagtg attcgccaag ctcttctgaa ctatccggat atttttgtct tttcagatga
     7561 ggaaaacttt gcacctcata ttctgacttt tggaatcaaa ggtgttcgag gtgaagtcat
     7621 cgttcacgcc tttgaagact atgatatttt catctcaaca acctcagctt gttcatctaa
     7681 ggcaggaaaa ccagccggta ccttgattgc catgggagtg gacaaagata aggccaagtc
     7741 agctgtgcgt cttagcctag acttggaaaa tgatatgagt caggtcgagc agtttttgac
     7801 caagttaaaa ttgatttaca atcaaactag aaaagtaaga taggagcatt catgcagtat
     7861 tcagaaatta tgattcgcta cggagagttg tcaaccaagg gtaaaaaccg tatgcgtttc
     7921 atcaataaac ttcgtaataa tatttcggac gttttgtcta tctataccca agttaaggta
     7981 acagcagatc gcgaccgtgc ccacgcttac ctcaatggag ctgattacac agcagttgca
     8041 gaatctctca aacaagtttt tggaattcaa aacttttctc ctgtttataa ggttgaaaaa
     8101 tctgtagaag ttttgaagtc ttctgtccaa gagattatgc gggacatcta caaggaaggt
     8161 atgaccttta agatttctag caagcgtagc gaccacaact ttgaacttga tagtcgtgaa
     8221 ctcaaccaaa cacttggagg ggctgtattc gaagccattc caaatgtgca agttcaaatg
     8281 aaaagtcctg acatcaatct tcaggtggag attcgtgaag aagcagccta tctttcttat
     8341 gaaaccattc gtggggctgg tggtttgcca gttggaactt caggtaaagg gatgctcatg
     8401 ttgtcaggag ggattgactc acctgtagca ggttatcttg ctcttaagcg tggggtggat
     8461 atcgaggcag ttcactttgc tagtccacca tatactagtc ctggtgccct caagaaagcg
     8521 caggacttga cccgtaaatt gaccaagttt ggcggaaata tccagtttat agaggtgcct
     8581 ttcacagaga ttcaagagga aatcaaagcc aaagcgccag aagcttattt gatgactcta
     8641 actcgtcgct ttatgatgcg gattactgac cgtattcgtg aggtacgaaa tggtttggtt
     8701 atcatcaatg gggaaagtct aggtcaagta gccagccaaa cccttgaaag tatgaaggct
     8761 atcaatgctg ttaccaacac tcccatcatt cgtcctgtgg ttaccatgga caagttggaa
     8821 atcattgaca tcgcccagga aatcgatacc tttgacattt caatccaacc gtttgaagac
     8881 tgttgtacca tttttgcacc agatcgtcca aaaacaaatc ctaaaattaa gaatgcggag
     8941 cagtacgaag cgcgtatgga tgttgaaggc ttggttgagc gagcagtggc tggaatcatg
     9001 attactgaaa tcacacctca agccgaaaaa gatgaagttg atgacttgat tgacaatctg
     9061 ctctaattca gaaaatccaa aagaatagcg aaaatcagta aaaaaagtta gttttttctc
     9121 taaaaacagg taaaaaacta acttttttta tttttatgat ataatgatat aaaattttga
     9181 atatagagag ttttctgaca atgaatcaat cctactttta tctaaaaatg aaagaacaca
     9241 aactcaaggt tccttataca ggtaaggagc gccgtgtacg tattcttctt cctaaagatt
     9301 atgagaaaga tacagaccgt tcctatcctg ttgtatactt tcatgacggg caaaatgttt
     9361 ttaatagcaa agagtctttc attggacatt catggaagat tatcccagct atcaaacgaa
     9421 atccggatat cagtcgcatg attgtcgttg ctattgacaa tgatggtatg gggcggatga
     9481 atgagtatgc ggcttggaag ttccaagaat ctcctatccc agggcagcag tttggtggta
     9541 agggtgtgga gtatgctgag tttgtcatgg aggtggtcaa gccttttatc gatgagacct
     9601 atcgtacaaa agcagactgc cagcatacgg ctatgattgg ttcctcacta ggaggcaata
     9661 ttacccagtt tatcggtttg gaataccaag accaaattgg ttgcttgggc gttttttcat
     9721 ctgcaaactg gctccaccaa gaagccttta accgctattt cgagtgccag aaactatcgc
     9781 ctgaccagcg catcttcatc tatgtaggaa cagaagaagc agatgataca gacaagacct
     9841 tgatggatgg caatatcaaa caagcctata tcgactcgtc gctttgctat taccatgatt
     9901 tgatagcagg gggagtacat ctggataatc ttgtgctaaa agttcagtct ggtgccatcc
     9961 atagtgaaat cccttggtca gaaaatctac cagattgtct gagatttttt gcagaaaaat
    10021 ggtaagttaa gaaaggaaaa aacgaaatgc atattgaaca tcttagccac tggagtggtc
    10081 atcttaaccg tgaaatgtac cttaaccgtt atggacatgg tgggattcca gttgtggtct
    10141 ttgcttcatc aggtggtagt cacaacgaat actatgattt tggcatgatt gatgcctgtg
    10201 cttcctttat cgaggaaggc cttgtccagt tctttaccct atctagtttg gatagtgaga
    10261 gctggttggc tacttggaaa aatgctcatg accaagcgga aatgcaccgt gcctacgaac
    10321 gttatgtgat tgaggaggcc attcttttat caagcacaag acaggttggt ttgatggcat
    10381 gatgacgaca ggttgctcta tgggagccta tcatgcactc aatttcttcc tccagcatcc
    10441 agatgtcttt accaaagtga ttgctctcag tggtgtttac gacgcacgtt tctttgtcgg
    10501 tgattactac aacgatgatg ctatttacca aaactcgcca gtagattata tttggaacca
    10561 aaacgacggc tggtttattg accgttaccg tcaggcagag attgtgctgt gtacggggct
    10621 tggagcctgg gaacaagatg gtttgccatc cttttacaag ctcaaagaag cctttgacaa
    10681 gaaacaaatt ccagcctggt ttgctgaatg gggacatgat gtcgcccatg actgggaatg
    10741 gtggcgtaaa caaatgcctt atttcctcgg taatctctat ttataaaagg agttacctat
    10801 gaattacctt gttatttctc cctactatcc acaaaacttt caacagttta ccatcgaact
    10861 agctaataaa ggcatcacag tcttgggaat tggtcaagag tcttacgagc aattggatga
    10921 gcccttgcgc aatagcttga ccgagtattt tcgtgttgat aatcttgaga acatagatga
    10981 agtcaaacgt gcagttgctt ttctctttta taaacatggt ccaattggcc gcatcgagtc
    11041 tcacaatgaa tactggcttg agctagacgc aacactcaga gaacaattca atgtttttgg
    11101 tgccaaacca gaggatctca aaaagacgaa atataagtct gaaatgaaga aacttttcaa
    11161 aaaagcaggt gttcctgtgg tacctggagc tgttatcaag acggaagcag atgttgatca
    11221 agcagtgaaa gaaatcggtc ttccaatgat tgccaaacct gataatggag tgggagcagc
    11281 cgcaaccttt aaacttgaga cagaagacga tatcaatcac ttcaagcaag aatgggacca
    11341 ttcaaccctt tatttctttg aaaaatttgt cacttccagc gaaatctgta cctttgacgg
    11401 gctcgtggac aaggatggaa agattgtctt ctcaacaacc tttgactacg cctatacacc
    11461 gcttgacctc atgatttata agatggacaa ttcttattat gtgctcaagg atatggatcc
    11521 taaactgcgc aagtatgggg aagcaattgt caaagaattt ggtatgaaag aacggttttt
    11581 ccatattgag ttcttccgtg agggggacga ttatattacc atcgagtaca ataaccgccc
    11641 tgcaggtggt tttaccattg atgtttataa ctttgctcat tccttggacc tttatcgtgg
    11701 ctatgcagct attgtcgcag gagaggagtt cccggcgtca gactttgaaa ctcagtattg
    11761 tttggctact tctcgccgtg caaatgctca ctatgtttat tcagaagagg atttgcttgc
    11821 caaatatagc cagcagttca aggttaaaaa agtcatgcca gctgccttcg cggaacttca
    11881 aggagattac ctgtatatgc tgaccactcc gagtcgacaa gaaatggagc agatgattgc
    11941 agatttcgga caacgtcaag aataagaact atcggattaa ggaaattaac tcccttaatc
    12001 cttttgtttt gtctgataaa aaataagagc atcccaacaa ggtagctatc ataaaacttg
    12061 ttcgataact atttgaagca ggattaggtg gtcagaaatt aaattttaat atttcaattg
    12121 agtcatagta ttgtgtttgc gtatccttaa atcagctaaa aggatccatg acgacaccta
    12181 tacgatatag ttttcaagat accaaacaag tctattaata ttcaatgaaa atcaaagagc
    12241 aaactaggaa gctagccgca ggtttctcaa aacactgttt tgaggttgtg gatagaactg
    12301 acagagtcag tatcatatac ctacggcaag gtgaagctga cgtggtttga agagattttc
    12361 gaagagtata aaatattcag gtgacgcata gatatagtta attgaagctt tgtttgaaat
    12421 ctgataaaat aatgatatta ctaagtttta aaaactaaag aaaagggaag atatgattac
    12481 aggcgaatta aaaaataaaa tcgatcagct gtgggaaatt ctttggacag aaggaaacgc
    12541 aaatccttta acaaatattg aacagttgac ttatctctta tttatgaaag atttggatag
    12601 tgtcgagctt ggacgtgaaa gtgatgctga atttctaggg attccttatg agggagtttt
    12661 tccaaaagat aaacctgaat accgttggtc aacttttaaa aatataggag atgctcagga
    12721 agtttatcgt ttaatgactc aggagatttt tccgtttatt aaaaatctca agggggatac
    12781 agatgataca gccttttcac gatatatgcg agaagctatt tttcaaataa ataaacctgc
    12841 tacgcttcaa aaggcaattt ctatcttaga tgtttttcca actaggggat tagatgtaga
    12901 ttttgataat gacaaacaaa gtattactga tatcggagat atctatgaat atctgttatc
    12961 aaaattgtcg accgcaggta aaaatggaca gttccgtaca cctcgtcaca tcatcgatat
    13021 gatggttgag ttgatgcaac cgactatcaa agatatcatc tcagatcccg ctatgggttc
    13081 tgctggcttc ttagtatctg ctagccgtta cttaaagcgt aagaaagatg aatgggaaac
    13141 caatacagat aatatcaatc attttcataa tcagatgttt catggaaatg atacggatac
    13201 gactatgttg agacttgggg cgatgaacat gatgctacat ggagtagaaa atccacaaat
    13261 cagttacctt gactcgctgt ctcaagataa tgaagaagcc gataaatata ctttggtttt
    13321 agcaaatcct ccttttaagg gctcacttga ctacaattca acctctaatg accttcttgc
    13381 aaccgtaaaa accaaaaaaa cagaattact ctttctttct cttttcttgc gaactttaaa
    13441 accaggtgga cgagcagcag ttatcgtacc tgatggtgtc ctttttggtt cgtctaaagc
    13501 tcataaagga attcgtcagg aaattgtaga gaatcataag cttgatgctg taatctcaat
    13561 gcctagtggt gtgttcaagc cttatgctgg agtttcaact gccattctca tctttacaaa
    13621 aactggtaat ggtggtactg acaaagtctg gttttacgat atgaaagcgg atggtttaag
    13681 tttggatgat aagcgacaac cgattagcga caatgatatt ccagatatta tcgaacgctt
    13741 tcatcatctt gaaaaagaag cagaacgtca gagaacggat caatctttct ttgttccagt
    13801 tgctgagata aaggaaaatg attatgattt gtctatcaat aaatataaag agattgagta
    13861 tgaaaaagtt gagtatgaac caacagaggt catcttaaag aaaatcaatg atttagaaaa
    13921 agaaattcaa gctggcttgg ctgaattaga aaaattactc aagtagggag gtggctgtat
    13981 gaaaaaagtg aagttggggg aagtcttatc tctaaaaaaa ggcaagaaag ccactgtact
    14041 tgctgaacaa acaactctaa gccaacgtta tattcaaata gatgatttaa gaaataataa
    14101 taatttaaaa ttcactgaaa gtttaaatat gactgaagca ctcccagatg atattctgat
    14161 agcatgggat ggagctaatg caggaacagt tggttatgga ttatcgggag ctgttggtag
    14221 tacaattacg gtcttaaaaa agaatgagcg atacaaagaa aaaattatat cagattactt
    14281 gggagtcttt ttggaaagta aatcgcagta tttacgagaa cattcaacag gtgcaacaat
    14341 tcctcattta aacaagaata tattacttga tttacaatta gaattgctag gtatcgaaga
    14401 acaagagaac attatctgta ttcttaatac gattaaaggg cttattacta aaagaaaatt
    14461 gcagttagat gaactaaact tgctcgtcaa atcccgattt aacgagatgt ttggggatcc
    14521 tttaaataat aacaagaaat ttgcagtaaa aacgggacaa cagtgtttta aattttcaag
    14581 tggaaaattt cttgataaa
//
LOCUS       DAVHEX010000052        14449 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVHEX010000052 DAVHEX010000000
VERSION     DAVHEX010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14449
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.052"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            55..597
                     /locus_tag="VVL45_001637"
     CDS             55..597
                     /locus_tag="VVL45_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEU4187791.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            complement(638..711)
                     /locus_tag="VVL45_001638"
     tRNA            complement(638..711)
                     /locus_tag="VVL45_001638"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(675..677),aa:Asn,seq:gtt)
     gene            complement(717..788)
                     /locus_tag="VVL45_001639"
     tRNA            complement(717..788)
                     /locus_tag="VVL45_001639"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(753..755),aa:Glu,seq:ttc)
     gene            complement(840..1592)
                     /locus_tag="VVL45_001640"
     CDS             complement(840..1592)
                     /locus_tag="VVL45_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4187792.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            complement(1589..2914)
                     /locus_tag="VVL45_001641"
     CDS             complement(1589..2914)
                     /locus_tag="VVL45_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU4187793.1"
                     /translation="MDLLGFGTVIVHFLIISHSYRLICKGRINRKELYVFGAYTLLTE
                     IVLEFSFYLLYLDKIGIERFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNF
                     LSVIFSSITGDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDKDYLYPFLKK
                     VFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIA
                     LKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEV
                     LVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIE
                     LLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFA
                     LGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
     gene            complement(2935..3060)
                     /gene="comC"
                     /locus_tag="VVL45_001642"
     CDS             complement(2935..3060)
                     /gene="comC"
                     /locus_tag="VVL45_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HEU4187794.1"
                     /translation="MKNTVKLEQFVALKEKDLQKIKGGEMRISRIILDFLFLRKK"
     gene            complement(3225..3298)
                     /locus_tag="VVL45_001643"
     tRNA            complement(3225..3298)
                     /locus_tag="VVL45_001643"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3262..3264),aa:Arg,seq:tct)
     gene            complement(3342..3821)
                     /gene="rlmH"
                     /locus_tag="VVL45_001644"
     CDS             complement(3342..3821)
                     /gene="rlmH"
                     /locus_tag="VVL45_001644"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEU4187795.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            4004..5185
                     /locus_tag="VVL45_001645"
     CDS             4004..5185
                     /locus_tag="VVL45_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU4187796.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVIVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            5243..6001
                     /locus_tag="VVL45_001646"
     CDS             5243..6001
                     /locus_tag="VVL45_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEU4187797.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            6214..7575
                     /gene="dnaA"
                     /locus_tag="VVL45_001647"
     CDS             6214..7575
                     /gene="dnaA"
                     /locus_tag="VVL45_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEU4187798.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYNYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
     gene            7734..8870
                     /gene="dnaN"
                     /locus_tag="VVL45_001648"
     CDS             7734..8870
                     /gene="dnaN"
                     /locus_tag="VVL45_001648"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEU4187799.1"
                     /translation="MIHFSINKNLFLQALNTTKRAISSKNAIPILSTVKIDVTNEGIT
                     LIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQN
                     QIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRP
                     ILTGVHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDD
                     IETVEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSME
                     RARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYL
                     IDSLKALNSEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN"
     gene            8935..9129
                     /locus_tag="VVL45_001649"
     CDS             8935..9129
                     /locus_tag="VVL45_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEU4187800.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            9213..10328
                     /gene="ychF"
                     /locus_tag="VVL45_001650"
     CDS             9213..10328
                     /gene="ychF"
                     /locus_tag="VVL45_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEU4187801.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELNDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            10399..10968
                     /locus_tag="VVL45_001651"
     CDS             10399..10968
                     /locus_tag="VVL45_001651"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEU4187802.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVNYYLQEKNFEKTMQRYNG"
     gene            10969..>14449
                     /gene="mfd"
                     /locus_tag="VVL45_001652"
     CDS             10969..>14449
                     /gene="mfd"
                     /locus_tag="VVL45_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEU4187803.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERDLAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTNICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNIDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKKDYEI
                     LEGLLIFGESLLEI"
BASE COUNT         4969 a         2416 c         2580 g         4484 t
ORIGIN      
        1 agataaaaaa tctattttta gacatatagt ctaatttgtt tacaaggagg aaatatgcaa
       61 gaaagtaaca aacgcttaaa aacaaagcga actattgaaa atgctatggt acaattactg
      121 atggaacagc catttgataa aatttctact gtcaaattag tagaaaaagc cggaattagt
      181 cgttccagct tctatactca ctataaggat aagtatgata tgattgagca ctatcaaagc
      241 aagctattcc atacatttga atatattttt caaaaacatg ctcatcacaa aagagacgct
      301 attttagaag tttttgaata tctagagtca gaaccacttc tggctgccct tctttctgaa
      361 aatgggacta aagaaatcca aaatttctta cgaaataaac ttcatatcat gcttagtaca
      421 gatttacaaa agcgatttat gcaactgaat ctaaatacca ctgaattaga atatagtagc
      481 atctatctaa ctcatgcact ttttggtgtc tgccaaactt ggattgcaca tggaaaaaaa
      541 gaaagttctc aagaaataac agacttcctt atgaaaatgc ttggtgatac aaattgatac
      601 aaaaagagga acacagctgt gttccctttt atctatactc cgccagtagg actcgaacct
      661 acgacatcat gattaacagt catgcgctac taccaactga gctatggcgg ataaaatagt
      721 ccgtacggga ttcgaacccg tgttaccgcc gtgaaaaggc ggtgtcttaa ccccttgacc
      781 aacggacctt ctatctgtag cagatataac cattatatca atttcttgct aattgtcaat
      841 cacttttgag attttttctc taaaatatct tttaattttc taatttttaa tcttgaaata
      901 ggacaacgat ggtcttcata gaaaacaatt tctaagtttt ttcgatcaat ttctctgata
      961 ttacctatat ttaccaaaaa tgacttgtga ggagaataaa atcgctgagt atgtttgtcc
     1021 ttttcctgaa tatctgtcat ggtaccataa aactcttttg caaaattctt accaataatg
     1081 cgcaatttat gagatacccc tgttgtttca atatacaaaa tatcatggta aggaattttt
     1141 aaatcatttc ccttgtaatt gtagtcgaaa taatctacaa catcttcatt ttcaagtaac
     1201 atactcttcg tgtagaagat attttgctca attctcttct taaacatctc atcattgata
     1261 tccttatcaa caaaatctag ggctgatacc tggtatttat aggttagagt cgcaaactct
     1321 gatcgactag tgataaagac gataatagcg taaggattgt aatgacgaat gagctgagcc
     1381 acttcaaatc cctttttctc aattccatga atatcgatat ctaggaaata aagctgattt
     1441 acttcatcat tttcaatgta ttcttcaaat tcacggactt ttcccgttgt cttgtatgat
     1501 attggaatat tcgattcttt cgaaatttca tccaatattc tctctagtct cacttgatgt
     1561 tcaataacat cttctaaaat taaaactttc attcaaattc cctcttaaat ctaatgattt
     1621 gtctaaatgt actgccttcc atctctgttt ctaaaataat attgttgtac ttatctagta
     1681 gttctttcac attatttaat ccgactccgc gatttcttcc cttagtggag aatcctaagg
     1741 caaatagatc tcctgaagga gtcatcgtca ttttacatga attctgaatc acaataactg
     1801 tttcagtttc catcttaata actgctactt ccatctgctt tttatagcta tcagccgatc
     1861 cttcgacagc attattcaat aaaacgctca tgatacgaac caaatccaat agttcaattg
     1921 gaagcttggt aatcgtatct tttacttcca gtgtaaactc tacaccatta tttcgagcat
     1981 agacaattga ctgagcaacc aaacttcgta aagctgagtc ttctatgttg ttcaaatcaa
     2041 agtaagtgta cttatctgaa cgcaatttat gatttgcttt gactaaaact tcattgtaaa
     2101 ttctgtcaat ttcctgtaaa ttaccactgt caattgccat ctgcatgctg acaagcattc
     2161 cagcataatc atgtcgaaaa ccacggattt cattatacag accaacaatt tcatctgtgt
     2221 aattctgtaa atgtttctgt tcaaatttct tctgcttcaa agcaatctct ttctccattt
     2281 gaactttatg agaattcatt gcaaagaagg tcaaaaggag agagataaag acaatagatg
     2341 acaaaatact tccaaaacta ttcaaatgtt taatcgtact taccatatct gaaacgaaag
     2401 atacaatatg tagcaatagt aaagcaaaaa atactttttt caagaaagga taaaggtagt
     2461 ccttgtcaaa ataggctagt tccaaatgga aatagtaaat gatttttaat gtaacaaaat
     2521 aggttaacac cgtcacaacg aaaaagaatg ggaaatgata ttgtaaaaca aaattatctc
     2581 ctgttataga ggagaaaatt acggacagaa agttatgagt gctctcatat aaaagagata
     2641 gtagtaaact taggaatagt cctctatccc tctcatactg tttcatccat cgaaaatagg
     2701 aatataagcc caaaggaaat aaaaatcttt caatccctat tttatctaaa tatagaagat
     2761 aaaaggaaaa ttcaagtact atttcagtta gtaatgtata agcaccaaaa acgtataatt
     2821 cttttctatt tattcgacct ttacaaatta aacggtaact gtgactaata attaaaaaat
     2881 gaacaataac tgtcccaaat ccaagtaaat ccattactct ttctccttat ttcattactt
     2941 ttttcgtagg aaaagaaaat caaggatgat tcttgaaatc ctcatctccc cacctttaat
     3001 cttttgtaag tctttttcct tcaaagctac aaactgttcc aatttaactg tgtttttcat
     3061 aataaaatct cctaaaatgt tttttcttgt aagctaactt acaaaaacca ttatacaaaa
     3121 tggaatttcg ttttagataa aattctctca actgtcattt ttttctccca aagtgtactt
     3181 ttttaagaaa aaagccggga aaattcccag ctttgctatt atattgatcc cagcaggatt
     3241 cgaacctgcg accgttcgct tagaaggcga atgctctatc cagctgagct atgagaccta
     3301 atacaattat tctaccaaaa attcaattaa aagtcaattt tctatttatg gtaggggaat
     3361 ccctgctgaa tcgtaaaagc gcgatagatt tgttcaacaa gaactagtct cattaactga
     3421 tggggtaagg ttaggcgacc aaaactgaca gaaagattgg ctctattttt tacagatgat
     3481 gataatccta aacttccccc aataataaaa gtaagagtag aaaatccttt tatagaagtt
     3541 tcttctaact gcttactaaa ttcttctgag aagaaagttt tcccttcaat ggctaacaca
     3601 ataacgaaat cacggtcagc aatttttgat aaaattctct gaccttctat ttctaaaatc
     3661 ttttgatttt ctgattcact ggccttatct ggtgtttttt catctgataa ctcaatcatt
     3721 tcaaacttag caaatctaga aattcgtttt gaatactctg cgataccatc ttttaaatac
     3781 ttttctttca gtttcccaac tgttacaact ttaattttca tgactctatt ctaacatatt
     3841 ctctattttt tcacatctta ttcacaaaat aaaaaataga tttcaattaa gaaaatcaca
     3901 atttcaaaag agttatccac agtttgtgta aaacttttgt gtttaagtta taattaagct
     3961 agtcagttta tactttcagt aattcaaaca tatggaggca aatatgaaac atctaaaaac
     4021 attttacaaa aaatggtttc aattattagt cgttatcgtc attagctttt ttagtggagc
     4081 cttgggtagt ttttcaataa ctcaactaac tcaaaaaagt agtgtaaaca actctaacaa
     4141 caatagtact attacacaaa ctgcctataa gaacgaaaat tcaacaacac aggctgttaa
     4201 caaagtaaaa gatgctgttg tttctgttat tacttattcg gcaaacagac aaaatagcgt
     4261 atttggcaat gatgatactg acacagattc tcagcgaatc tctagtgaag gatctggagt
     4321 tatttataaa aagaatgata aagaagctta catcgtcacc aacaatcacg ttattaatgg
     4381 cgccagcaaa gtagatattc gattgtcaga tgggactaaa gtacctggag aaattgtcgg
     4441 agctgacact ttctctgata ttgctgtcgt caaaatctct tcagaaaaag tgacaacagt
     4501 agctgagttt ggtgattcta gtaagttaac tgtaggagaa actgctattg ccatcggtag
     4561 cccgttaggt tctgaatatg caaatactgt cactcaaggt atcgtatcca gtctcaatag
     4621 aaatgtatcc ttaaaatcgg aagatggaca agctatttct acaaaagcca tccaaactga
     4681 tactgctatt aacccaggta actctggcgg cccactgatc aatattcaag ggcaggttat
     4741 cggaattacc tcaagtaaaa ttgctacaaa tggaggaaca tctgtagaag gtcttggttt
     4801 cgcaattcct gcaaatgatg ctatcaatat tattgaacag ttagaaaaaa acggaaaagt
     4861 gacgcgtcca gctttgggaa tccagatggt taatttatct aatgtgagta caagcgacat
     4921 cagaagactc aatattccaa gtaatgttac atctggtgta attgttcgtt cggtacaaag
     4981 taatatgcct gccaatggtc accttgaaaa atacgatgta attacaaaag tagatgacaa
     5041 agagattgct tcatcaacag acttacaaag tgctctttac aaccattcta tcggagacac
     5101 cattaagata acctactatc gtaacgggaa agaagaaact acctctatca aacttaacaa
     5161 gagttcaggt gatttagaat cttaattgac atctatgtaa agaaagcttt acataagaga
     5221 aaagatgtgt tagtgtagaa tcatggaaaa atttgaaatg atttctatca cagatataca
     5281 aaaaaatccc tatcaacccc gaaaagaatt tgatagagaa aaactagatg aactagcaca
     5341 gtctatcaaa gaaaatgggg tcattcaacc gattattgtt cgtcaatctc ctgttattgg
     5401 ttatgaaatc cttgcaggag agagacgcta tcgggcttca cttttagctg gtctacggtc
     5461 tatcccagct gttgttaaac agatttcaga ccaagagatg atggtccagt ccattattga
     5521 aaatttacag agagaaaatt taaacccaat agaagaagca cgcgcctatg aatctctcgt
     5581 agagaaagga ttcacccatg ctgaaattgc agataagatg ggcaagtctc gtccatatat
     5641 cagcaactcc attcgtttac tttccttgcc agaacagatt ctttcagaag tagaaaatgg
     5701 caaactatca caagcccatg cgcgttccct agttgggtta aataaggaac aacaagacta
     5761 tttctttcaa cggattatag aagaagatat ttctgtaagg aaattagaag ctcttctgac
     5821 agagaaaaaa caaaagaaac agcaaaaaac taatcatttc atacaaaatg aagaaaaaca
     5881 gttaagaaaa ctactcggat tagatgtaga aattaaacta tctaaaaaag acagtggaaa
     5941 aatcattatt tctttttcaa atcaagaaga atatagtaga attatcaaca gcctgaaata
     6001 aggctgttct tttatttttt tatctcacaa ggttatccac tatgtttttc gataaaaagc
     6061 ttaataaatc aataatttct tcttttatcc ccaacctgtg gataaagttt ggtaacattg
     6121 tggattattt ttcacagctt gtggaaaatt cttgctatct atggtaaaat atctctagta
     6181 ttaaactttt aaatagtaaa ggaggagaaa ggattgaaag aaaaacaatt ttggaatcgt
     6241 atattagaat ttgcacaaga aagactgact cgatccatgt atgatttcta tgctattcaa
     6301 gctgaactca tcaaggtaga ggaaaatgtt gccactatat ttctacctcg ctctgaaatg
     6361 gaaatggtct gggaaaaaca actaaaagat attattgtag tagctggttt tgaaatttat
     6421 gacgctgaaa taactcccca ctatattttc accaaacctc aagatacgac tagctcacaa
     6481 gttgaagaag ctacaaattt aactctttat aactatagtc caaagttagt atctattcct
     6541 tattcagata cgggattaaa agaaaagtat acctttgata actttattca aggggatgga
     6601 aatgtttggg ctgtatcagc cgctttagct gtctctgaag atttggctct gacctataac
     6661 cctcttttta tctatggagg accaggcctt ggtaagactc acttattaaa cgctattgga
     6721 aatgaaattc taaaaaatat tcctaatgcg cgtgttaaat atatccctgc cgaaagcttt
     6781 attaatgact ttcttgatca cctaagactt ggggaaatgg aaaagtttaa aaagacctat
     6841 cgtagtcttg atcttttgtt aatcgatgat atccagtcac tcagcggaaa aaaagtcgca
     6901 actcaggaag aatttttcaa tacctttaac gcccttcatg acaagcaaaa acagattgtc
     6961 ctaacgagtg atcgtagtcc aaaacatcta gaagggctcg aggagaggct tgtcacgcgt
     7021 tttagttggg gattgacaca aactatcacc ccccctgact ttgaaacacg tattgccatt
     7081 ttacaaagta agacggaaca tttaggctac aatttccaaa gtgatactct agaataccta
     7141 gctgggcaat ttgattcaaa tgttcgagat cttgagggag ccatcaacga catcacttta
     7201 attgccagag taaaaaaaat caaggatatc actattgata ttgctgcaga agccattaga
     7261 gcccgcaaac aagatgttag ccaaatgctc gtcatcccaa ttgataaaat ccaaactgaa
     7321 gttggtaact tttatggtgt tagtatcaaa gaaatgaagg gaagtagacg ccttcaaaat
     7381 attgttttgg cccgtcaagt agccatgtat ttatctagag aactaacaga taatagtctt
     7441 ccaaaaattg ggaaggaatt tgggggaaaa gatcatacca cagtcattca tgcccatgcc
     7501 aaaataaaat ctttgattga tcaagacgat aatttacgtt tagaaattga atcaatcaaa
     7561 aagaaaatca aataatttgt ggataacttt tagtttttta tcttttttat ccacattttt
     7621 taaacaagct aaaaaacttg atatgacttg tttaaaggct gttttccaca gatttcacag
     7681 actctattat tactattatc tttctaatac taaaaataaa taaaggagaa tccatgattc
     7741 atttttcaat taataaaaat ttatttctac aagcattaaa tactactaag agagctatta
     7801 gttctaaaaa tgccattcct attttatcaa cagtaaaaat tgacgtgacc aatgaaggta
     7861 ttactttaat tggttcaaat ggtcaaattt caattgaaaa ttttatttct caaaaaaatg
     7921 aagatgctgg tttgttaatt acttctttag gttcgatcct tcttgaagct tctttcttta
     7981 tcaatgtagt atctagttta cctgatgtaa ctcttgattt taaagaaatt gaacaaaatc
     8041 aaattgtttt aaccagtggc aaatcagaaa ttaccctaaa aggaaaagat agcgaacaat
     8101 atccacgaat ccaagaaatt tcagcaagca ctcctttaat acttgaaaca aaattactca
     8161 agaaaattat taatgaaaca gcctttgctg caagtacaca agagagtcgt ccgattttaa
     8221 caggtgtcca cttcgtattg agtcaacaca aagagttaaa aacagttgca acagactctc
     8281 atcgcctaag ccagaaaaaa ttgactcttg aaaaaaatag tgatgatttt gatgtcgtaa
     8341 ttcctagccg ttctctacgc gaattttcag cggtatttac agatgatatc gaaactgtag
     8401 agattttctt tgccaataac caaatcctct ttagaagcga aaatattagc ttctatactc
     8461 gtctcctaga aggaaactat cctgatacag atcgcttgat tccaacagac tttaacacta
     8521 ctattacttt taatgtggta aacttacgcc agtcaatgga gcgtgcccgt cttttatcaa
     8581 gtgcgactca aaatggtact gtgaaacttg aaattaagga tggggttgtt agcgcccatg
     8641 ttcactctcc agaagttggt aaagtaaacg aagaaatcga tactgatcag gttactggtg
     8701 aagatttgac cattagtttc aacccaactt acttgattga ttctcttaaa gctttaaata
     8761 gcgaaaaggt gactattagc tttatctcag ctgttcgtcc atttactctt gtgccagcag
     8821 atactgacga agacttcatg cagctcatta caccagttcg tacaaattaa gtgaaagagg
     8881 ttgagcctgg ctcgcctctt ttatgatata atcgaaaaag aaaaggagag tagtatgtat
     8941 caagttggaa attttgttga gatgaaaaaa tcacacgctt gtacaatcaa gtcgactggt
     9001 aaaaaggcta atcgttggga aattacacgt gtaggagcag atatcaaaat aaaatgtagt
     9061 aattgtgagc atgttgtcat gatggggcga tatgattttg agcgaaaaat gaataaaatt
     9121 attgactgag aacccttagt tagagggtta gcactttatc cctttttgtg ttataatatt
     9181 agggattgaa atgaaaacgg agaatgagaa atatggcttt gacagcaggt atcgttggtt
     9241 tgccaaacgt tggtaaatca acactattta atgcaattac aaaagcagga gcagaggcag
     9301 caaactaccc atttgcgacg attgatccaa atgttggaat ggtggaagtt ccagatgaac
     9361 gcctacaaaa actaactgaa atgataactc ctaaaaagac agttcccaca acatttgaat
     9421 ttacagatat tgcagggatt gtaaaaggag cttcaaaagg agaggggcta gggaataaat
     9481 tcttggccaa tattcgtgaa gtagatgcga ttgttcacgt agttcgtgct tttgatgatg
     9541 aaaatgtaat gcgcgagcaa ggacgtgaag acgcctttgt agatccactt gcagatattg
     9601 ataccattaa tctggaattg attcttgctg acttagaatc agtgaacaaa cgatatgcgc
     9661 gtgtagaaaa gatggcacgt acgcaaaaag ataaagaatc agtagcagaa ttcaatgttc
     9721 ttcaaaagat taaaccagtc ctagaagacg ggaaatcagc tcgtaccatt gaatttacag
     9781 atgaggaaca aaaggttgtc aaaggtcttt tccttttgac gactaaacca gttctttatg
     9841 tagctaatgt ggacgaggat gtggtttcag aacctgactc tatcgactat gtcaaacaaa
     9901 ttcgtgaatt tgcagcgaca gaaaatgctg aagtagtcgt tatttctgcg cgtgctgagg
     9961 aagaaatttc tgaattgaat gatgaagata aaaaagagtt tcttgaagcc attggtttga
    10021 cagaatcagg tgtagataag ttgacgcgtg cagcttacca cttgcttgga ttgggaactt
    10081 acttcacagc tggtgaaaaa gaagttcgcg cttggacttt caaacgtggt atgaaggctc
    10141 ctcaagcagc tggtattatc cactcagact ttgaaaaagg ctttattcgt gcagtaacca
    10201 tgtcatatga agatctagtg aaatacggat ctgaaaaggc cgtaaaagaa gctggacgct
    10261 tgcgtgaaga aggaaaagaa tatatcgttc aagatggcga tatcatggaa ttccgcttta
    10321 atgtctaaaa attaataaat ggtgtcaatt aggttggaaa aaaattccaa cccttttggc
    10381 ttttgaaagg aaaaataaat gaccaaatta cttgtaggct tgggaaatcc aggggataaa
    10441 tattttgaaa caaaacacaa tgttggtttt atgttgattg atcaactagc gaagaaacag
    10501 aatgtcactt ttacacacga taagatattt caagctgacc tagcatcctt tttcctaaat
    10561 ggagaaaaaa tttatctggt taaaccaacg acctttatga atgaaagtgg aaaagcagtt
    10621 catgctttat taacttacta tggtttggat attgacgatt tacttatcat ttacgatgat
    10681 cttgacatgg aagttgggaa aattcgttta agagcaaaag gctcagcagg tggtcataat
    10741 ggtatcaagt ctattattca acatatagga actcaggtct ttaaccgtgt taagattgga
    10801 attggaagac ctaaaaatgg tatgtcagtt gttcatcatg ttttgagtaa gtttgacagg
    10861 gatgattata tcggtatttt acagtctgtt gacaaagttg acgattctgt aaactactat
    10921 ttacaagaga aaaattttga gaaaacaatg cagaggtata acggataaat ggtgacctta
    10981 ttagatttat tctcagaaaa tgatcagatt aaaaaatggc atcaaaattt aacagataag
    11041 aaaagacaac taatacttgg tttatcaaca tctactaagg ctcttgcaat tgcaagcagt
    11101 ttagaaaaag aagataggat tgtgttattg acgtcaactt atggagaagc agaaggactt
    11161 gttagtgatc ttatttctat cttgggtgag gaactcgtct atccattttt ggtagatgat
    11221 gctcctatgg tggagttttt gatgtcttca caggaaaaaa ttatttcacg ggttgaagcc
    11281 ttgcgttttt tgactgattc atctaagaaa gggattttag tttgtaatat cgcagcaagt
    11341 cgattgattt taccgtctcc caatgcattc aaagatagta ttgtaaaaat ctcagttggt
    11401 gaagaatatg atcaacacgc gtttatccat cagttaaagg aaaatggcta tcgaaaagtt
    11461 actcaagtac aaactcaggg cgaatttagt cttcgaggag atattttaga tatttttgaa
    11521 atatcccagt tagaaccttg tcgaattgag ttttttggtg atgaaattga tggtatcagg
    11581 tcatttgaag tagaaacaca attatcgaaa gaaaataaga cagaactcac tatctttcca
    11641 gctagtgata tgcttttgag agaaaaggat tatcaacgag gacagtcagc tttagaaaaa
    11701 caaatttcaa aaactttatc acctattttg aaatcatacc tagaagaaat tctttcaagt
    11761 tttcaccaaa aacaaagtca tgcagactct cggaagtttt tatctttgtg ctatgataag
    11821 acatggactg tctttgatta tattgaaaaa gatactccaa tattctttga tgattatcaa
    11881 aaattgatga atcagtatga agtctttgaa agagacttag cgcagtactt tacagaagaa
    11941 ttacagaata gtaaagcatt ttctgatatg cagtattttt ctgatattga acaaatctat
    12001 aaaaaacaaa gtccagtgac ctttttctct aatcttcaaa agggtttagg aaatctcaaa
    12061 tttgacaaaa tttatcaatt caatcaatat cctatgcagg aatttttcaa tcagttttct
    12121 tttctaaaag aagaaattga acgatataaa aaaatggatt acaccattat tctgcagtct
    12181 agcaattcaa tgggaagtaa aacattggag gatatgttag aggaatatca gattaaattg
    12241 gattctagag ataagacaaa tatctgtaaa gaatctgtaa acttaataga gggtaatctc
    12301 agacatggtt ttcattttgt agatgaaaag attttattga taactgaaca tgagattttt
    12361 caaaagaaat taaagcgtcg ttttcgaaga caacatgttt caaatgcaga gagattaaaa
    12421 gattacaatg aacttgaaaa aggggactat gttgtccatc atatccatgg gattggtcaa
    12481 tatctaggaa ttgaaaccat tgaaatcaag ggaattcatc gcgattatgt cagtgtccaa
    12541 taccaaaatg gtgatcaaat ttctatcccc gtggaacaga ttcatctact gtccaaatat
    12601 atttcaagtg atggtaaagc tccaaaactc aataaattaa atgacggtca ttttaaaaag
    12661 gccaagcaaa aggttaagaa ccaggtagag gatatagctg atgatttaat caaactctac
    12721 tctgaacgta gtcagttgaa gggttttgct ttctcagctg atgatgatga tcaagatgcc
    12781 tttgatgatg ctttccctta tgttgaaacg gatgatcaac ttcgtagtat tgaggaaatc
    12841 aagagggata tgcaggcttc tcagccaatg gatcgacttt tagttgggga tgttggtttt
    12901 ggaaagactg aagttgctat gcgtgcagcc tttaaagcag tcaatgatca caaacaggtt
    12961 gtcattctag ttccgacgac ggttttagcg caacagcact atacgaattt taaggaacga
    13021 ttccaaaatt ttgcagttaa tattgatgtg ttgagtcgct ttagaagtaa aaaagagcag
    13081 actgcaacac ttgaaaaatt gaaaaacggt caagtcgata ttttgattgg aacacatcgt
    13141 gttttgtcaa aagatgttgt gtttgctgat ttgggcttga tgattattga tgaggaacag
    13201 cgatttggtg tcaagcataa ggaaactttg aaagaactga agaaacaagt ggatgtccta
    13261 accttgaccg ctacgccaat ccctcgtacc ctccatatgt ctatgctggg aatcagagat
    13321 ttatctgtta ttgaaactcc gccgactaat cgctatcctg ttcagaccta tgttttggaa
    13381 aagaatgata gtgtcattcg tgatgctgtc ttgcgtgaaa tggagcgtgg aggtcaagtt
    13441 tattatcttt acaacaaagt tgacacaatt gttcagaagg tttcagaatt acaggagttg
    13501 attccggagg cttcgattgg atatgttcat ggtcgaatga gtgaagtcca gttggaaaat
    13561 actctattag actttattga gggacaatac gatatcttgg tgacgactac tattattgag
    13621 acaggggtgg acattccaaa tgctaatact ttatttattg aaaatgcgga ccatatgggc
    13681 ttgtcaacct tatatcagtt aagaggaaga gtcggtcgta gtaatcgtat tgcttatgct
    13741 tatctcatgt atcgtccaga aaaatcaatc agtgaagtct ctgaaaagag attagaagcg
    13801 attaaaggat ttacagaatt gggctctggc tttaagattg caatgcgaga tctttcgatt
    13861 cgtggagcag gaaatctttt aggaaaatcc cagtctggtt tcattgattc tgttggtttt
    13921 gaattgtatt cgcagttatt agaggaagct attgctaaac gaaacggtaa tgctaacgct
    13981 aacacaagaa ccaaagggaa tgctgagttg attttgcaaa ttgatgccta tcttcctgat
    14041 acttatattt ctgatcaacg acataagatt gaaatttaca agaaaattcg tcaaattgac
    14101 aaccgtgtca attatgaaga gttacaagag gagttgatag accgttttgg agaataccca
    14161 gatgtagtag cctatctgtt agagattggt ttggtcaaat catacttgga caaggtcttt
    14221 gttcaacgtg tggaaagaaa agataataaa attacaattc aatttgaaaa agtcactcaa
    14281 cgactgtttt tagctcaaga ttattttaaa gctttatccg taacgaactt aaaagcaggc
    14341 atcgctgaga ataagggatt aatggagctt gtatttgatg tccaaaataa gaaagattat
    14401 gaaattttag aaggtttgct gatttttgga gaaagtttat tagagataa
//
LOCUS       DAVHEX010000053        14408 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVHEX010000053 DAVHEX010000000
VERSION     DAVHEX010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14408
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.053"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..157
                     /locus_tag="VVL45_001653"
     CDS             <1..157
                     /locus_tag="VVL45_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU4187804.1"
                     /translation="YSNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERT
                     KFVLSQA"
     gene            complement(207..917)
                     /gene="deoD"
                     /locus_tag="VVL45_001654"
     CDS             complement(207..917)
                     /gene="deoD"
                     /locus_tag="VVL45_001654"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU4187805.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLDDAVCFNEVRN
                     MFGYTGTYKGHCVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKKLGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(1571..2083)
                     /locus_tag="VVL45_001655"
                     /pseudo
     CDS             complement(1571..2083)
                     /locus_tag="VVL45_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
     gene            complement(2108..3088)
                     /locus_tag="VVL45_001656"
                     /pseudo
     CDS             complement(2108..3088)
                     /locus_tag="VVL45_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855254.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
     gene            complement(3564..4373)
                     /locus_tag="VVL45_001657"
     CDS             complement(3564..4373)
                     /locus_tag="VVL45_001657"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU4187806.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            complement(4389..4931)
                     /locus_tag="VVL45_001658"
     CDS             complement(4389..4931)
                     /locus_tag="VVL45_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEU4187807.1"
                     /translation="MTRYALLVRGINVGGKNKVVMAELRQELTNLGLEKVESYINSGN
                     IFFTSIDSKAQLVEKLETFFAVHYPFIQIFSLLSLEDFEAELENLPAWWSRDLARKDF
                     LFYTEGLDVDQVIATVENLELKDEVLYFGKLGIFWGKFSEESYSKTAYHKYLLKVPFY
                     RHITIRNAKTFDKIGQMLKK"
     gene            complement(4933..6144)
                     /locus_tag="VVL45_001659"
     CDS             complement(4933..6144)
                     /locus_tag="VVL45_001659"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEU4187808.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLDVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            complement(6158..6841)
                     /gene="rpiA"
                     /locus_tag="VVL45_001660"
     CDS             complement(6158..6841)
                     /gene="rpiA"
                     /locus_tag="VVL45_001660"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEU4187809.1"
                     /translation="MENLKKMAGITAAEFIKDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTSKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            complement(7139..7978)
                     /locus_tag="VVL45_001661"
     CDS             complement(7139..7978)
                     /locus_tag="VVL45_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEU4187810.1"
                     /translation="MKVIDQTLLEKVIIERSCTSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPEAMAFSLQDQQLLQEQLEKAEVVLLGPGL
                     RDDTFGENLVKQVFASLKKNQILIVDGGALTILARTSLLFPSNQLILTPHQKEWEKLS
                     GIAIEKQNEGTTSSALTSFPQGTILVEKGPATRIWQVGQSDYYQLKVGGPYQATGGMG
                     DTLAGMIAGFAGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISNCLPKVMK
                     RYV"
     gene            complement(7993..8814)
                     /locus_tag="VVL45_001662"
     CDS             complement(7993..8814)
                     /locus_tag="VVL45_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATPase"
                     /protein_id="HEU4187811.1"
                     /translation="MILKIYNGEYSLQWDGIYYLALIDYPNIQEWELEKIAKFIAYEK
                     LHKRQTSIECADSCLKKEILDYICQHPFLPPFTPTDKRVASTYDLHKRLVTSDYCSHT
                     TTIDAAISIFKTGRLLSAVKAFGRDAEELVLDSRNAASDPIDYFDYVMLGWSNTSSGY
                     RLAMERLLGRAPSEKELQDKFIPGVSFHFIYTDLIKVPGYIFDGYHAVKIKDMLNLLS
                     ELYICIIPTHNKSQFENIIPTKIQDRVYYLDYAGEDLEEWTKKVYQVVLKQSDKG"
     gene            complement(8952..9809)
                     /locus_tag="VVL45_001663"
     CDS             complement(8952..9809)
                     /locus_tag="VVL45_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEU4187812.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETGLVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGFRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLSKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            complement(9988..10722)
                     /locus_tag="VVL45_001664"
     CDS             complement(9988..10722)
                     /locus_tag="VVL45_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU4187813.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKVIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGDSKAVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQGQRTKDFLSKVL"
     gene            complement(10722..11405)
                     /locus_tag="VVL45_001665"
     CDS             complement(10722..11405)
                     /locus_tag="VVL45_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU4187814.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            11595..11825
                     /locus_tag="VVL45_001666"
     CDS             11595..11825
                     /locus_tag="VVL45_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEU4187815.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            12054..14312
                     /locus_tag="VVL45_001667"
     CDS             12054..14312
                     /locus_tag="VVL45_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU4187816.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRSSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPSISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
BASE COUNT         4323 a         3228 c         2603 g         4254 t
ORIGIN      
        1 ctattctaat gccaaactgg aagcgaccaa taatctcatc aaacttatca agcgcaatgc
       61 ctttggtttt cggaactttg aaaacttcaa aaaacggatt tttatcgccc tgaatatcaa
      121 aaaagaaagg acgaaatttg tcctttctca agcttagctt tttttcaacc cactacagtt
      181 gacaaagagc ccctttttgg ctataattat tctgcaatca aggtttccaa accaaccttc
      241 atcatatcag tgaaggtatt ttgacgttct tctgcagttg tgtcttcgtc tggattgacc
      301 aagctatcag agatggtcat gatagctagc gcatcaacat ggtattgggc agcaagatag
      361 taaagagctg ctgcttccat ttccacagcc ttgactcccc atttaccaag ctcgatattc
      421 ttttcaaagt aatttgagta aaagacatca gatgacaaaa cgttcccaac gtgagtagtc
      481 ataccaagtt ttttggcgat atggtaggct ttatcaagca aatcaaagct agcaatttgt
      541 ggaaaatcgt actgtggcca gtcattacga acgatgtttg agttggttgc agccgcctgc
      601 gccaaaacta attcacgaac atgaacctct tcattcaaag aacctgcagt tcccacacga
      661 atcaatttct tcacaccgta gtctacgatt aactcacgcg cataaatcga aatagatggc
      721 attcccatcc cagttcccat gacagataca cagtgaccct tgtaagtacc agtgtaacca
      781 aacatgttac gcacttcgtt aaaacaaaca gcatcatcaa ggaaattctc cgcaataaac
      841 ttagcacgaa gaggatcccc aggaagaaga attttatcag caatttcacc ctgctgagca
      901 gcaatatgga tagacataat ttatgataca aagagcgaga agaaaacgac tgaaaattag
      961 gaacctgacg agaaatcctg atttttcagt cagattatct attttccgag ttttccgctc
     1021 gtgttcaaat caaaacacac gctctacctt tctttatttt atattttata ttgagaaaga
     1081 taccaaaccc atcaaaaagc gaagggaaaa taggagttgg gcgcagtgag cgatgctcgc
     1141 tagatcaact atctttttcc cactactttt agggtggggt caattccttt cttaattttg
     1201 atttagagga gagtcgcccg tattcagttc agcgaataca gtttacccat cctttcgttt
     1261 ttgtttttag aaaagttttc tactcgtgtt caaattagaa cacgcgctct acctttctgt
     1321 ttatactctt cgaaaatctc ttcaaaccac gtcaacgtcg ccttggatta tatatgtgac
     1381 tgacttcgtc agtcttatct acaacctcaa agcagtgctt tgagcaacct gcggctagtt
     1441 tcttagtttg ctctttgatt ttcattgagt attaattttg ttctttcata gagagctacc
     1501 tgagtcctat tcaagctcag gtagtacttt cttataaact agacaaacta actgtcattc
     1561 taccatcaga ttacaagaca tcatcgtcac tcaccttgga attcaatgtc gtaccccaat
     1621 gggtaatttt acggtggggt tgagctaaaa ttggtctgtt ttcatagatt gtttgccatc
     1681 tattccatag taggcccgtc tttttctcaa tcttaactcg cagatttctc atattttctt
     1741 tgattgggag gttgaggaca aaacctgcag tctggttgcg accgcttcct tcccaagaat
     1801 gactacgaac aacttggttt ccatctttat ctactggaac ttcttcccaa gttatggagt
     1861 agcgggcaat gtaagctcca ctgtgttgaa ttgtcaatgt tttatccttc acaggagtct
     1921 gactgattgg ttgaactggc ttagaaactt gtgtcgccgt ttcagcattc gtagctataa
     1981 agcagaaaac ttcaacttct gcaagactga gggcattata gccttctagc taaatccgaa
     2041 catagcgcgc ttttttatgg ttaatcgtaa tttgtgctga cacatcttta ggagatgtta
     2101 tacgttttca ctcaatttct ttaccagaac tgtctaaaag aatgacatca aagtttgcca
     2161 atctatcctg ggcagtgtct gttcggttgt aaatattgat ttggcgaatg gtttcttctt
     2221 tagccaaatc tacttgccac caaggcttag attggaagtt tgtatgagtg acagaattgt
     2281 gaccatagtt accatcgact ttgccatcca cagctcttct agcatctcct ccgaaagctg
     2341 tcgaactttg agtaacctgt tttcctgagg caatattttc cattggttca gcgcttggtt
     2401 gactagaaga tcgaggcaga gcctgtttct taaagaatcg cactttatcc aatttcggat
     2461 cacgataacc atgtttatcc aaggtagctt gtttaccaat attaaagtta ggaatatcat
     2521 tcattggact ttcaaaatag ccacgagaac ggtgtttaaa gcgtgaatta cctgtaaatg
     2581 taacaatatc acgattatta tgaatcaagg gagtcacaac attgtcatta tcaaccacat
     2641 aatgcttaat ttttccacta acagctaatt tacgactttc caaaccaaca tcccagaaac
     2701 cattcttagc attccaaaca gttctggaag taatcacttt aggagcactg aaagttgtca
     2761 ctttcctcaa tacatggtta taaaaatcag gatatttttg gtaagcttca accgctgcaa
     2821 tctgagctag gtaacatcca agagaatgac ctgtcatata caacttagta atacttatat
     2881 ccttggctaa ttccccaaca accttgcgga tatcatccgc ttgagctggt tatgttatct
     2941 ctacttttga ctcttccctt ttgttttcaa gaacttcact tgcttccgga agcgtttgag
     3001 gagctagtct tggctcctct ctaagaggag tttctagttt tggagcatca ttttctgact
     3061 ctttcaattc ctccttagaa tctagcacac ctattaccac ttcagatgtt acatcttgct
     3121 ttacttcatt ggctgaaatt ccagctggag ctagaaatca aagccgacta caacagatac
     3181 aactccgata cttaattttt tatgccaaag cgcataattc gttgaccaat tttcattatt
     3241 tcttatcttt cttttcgttt atttacaagc agactcaagc gaaatctcat tgtgctatat
     3301 atgataagac tgagaaattc agaaagtcaa ttcattctca tctctcctta tcatactagt
     3361 ctgcttggct aggaagctaa tgctagtatc ctagaatctt gtctttcaca atgctggcta
     3421 tcagatcaga catacctatt cttccgcttc tttcagttat aagagcagga agaagctcat
     3481 agtattcctc tgtttcttct ttaatccgga taggcagtca atcctgaaaa aacagaggca
     3541 ctcccccttt taaatctttt ttcttacaat tcagcaagaa tcgctttaag caagcctttg
     3601 aaatcacctt taacacgttc agtcacttct acaacttctt cgtgattgag ttcttcttgg
     3661 aaaccagccg caaagttagt gatacatgaa attcccagaa ctttcaagcc agagtgggct
     3721 gccacgataa cttcaggaac cgtagacata ccaactgcat ctgctcccag tgtcttatag
     3781 gaacgaattt ctgctggtgt ttcataagtc ggaccagtaa ctccgatata gacaccttca
     3841 tcaagcttga tattaagttt tttagccact tcatgggcag tggcacggta ttctggtgtg
     3901 taggccctag acatatctgg gaaacgtggg ccaaagtcat ccaagttttc acccatcaat
     3961 ggattttgcc ccgtcatgtt gatatggtct gagatagcca tcaaggtacc aggaccaaat
     4021 ccgataccgc cagctgcatt ggttacaata acaccttcac atccaagaac tttcatcaca
     4081 cgaactggga aagtcaccac ttccagagga ttcccttcat agaaatggaa acgaccttga
     4141 agagccaaga ccttgcgacc tgccagttca ccatatacca atttaccagc atgaccgact
     4201 actgttgaac ggccccagtt tggaatctca gcatagtcta ctacaactgg attttcgatt
     4261 tcttctgcca attctccaag tcctgaccca aggattagac cgaactcagg ggctgcaatt
     4321 cccttttctt tcaggaaagt agcagtttca tggattttgt ttaaaaatgt cattgtgtgt
     4381 ctcctttatt atttttttag catttgacca attttgtcaa aggttttagc attacgaata
     4441 gtaatgtggc ggtagaaagg caccttcagc aagtacttat gataggcagt cttagaatag
     4501 gattcttcag aaaatttccc ccagaaaatc ccaagttttc caaaataaag cacttcatct
     4561 ttcagctcta aattttcaac tgtcgcgatg acttggtcca catccaaacc ctcagtgtaa
     4621 aagagaaaat cttttcgtgc caagtctctg ctccaccaag ctggtagatt ttcaagttcc
     4681 gcctcaaagt cctctagact cagtaaagaa aagatctgaa taaatggata atggactgca
     4741 aagaaagtct ctagcttttc aaccaattgg gctttggaat ctatcgaagt aaagaaaata
     4801 ttgccactgt tgatgtagct ctcaaccttt tccagtccta agtttgtcaa ttcttgacga
     4861 agctccgcca tgacgacctt attcttacca ccaacattga tacctctcac cagcaaagca
     4921 tagcgcgtca tcttatacca atttatctaa gaaactttcc ccaatcatag cagtttccac
     4981 accaaagtta tcggcaacag tcgctgaaat atctgcaaaa tgtcctactg gaatgagacc
     5041 atttccttta aaggcagggc tataggccaa caatggaata tattcccgag tgtgatccgt
     5101 tcctgcatac gttgggtcat ttccatggtc cgcagtaatc aagagaaggt cattctctct
     5161 catagctgcg ataatttcag gtaagcgttc atcaaactca tgcaagcaat cacggtaacc
     5221 gtgagcatta cgacgatggc cgtaaagggc atcaaagtca actaggtttg tgaatgagaa
     5281 tcctttttca aactcagcaa gtcccatagt cttcaatagt gtatcaattc catgactatt
     5341 tgacttgttg tgacccatgt catggttgat accagcaccg ttaaagatat cgttgatttt
     5401 acccacagca taagtatcga taccagcctc attcaattta tccaaaacag ttggggaaaa
     5461 tggagataca gccaagtcac gacggtttgc cgtacgagtg aagttacctg gttcacctac
     5521 ataagggcga gcaatgatgc gaccaagaag ggcaggacgc tcaagggtaa tcgaacgagc
     5581 gtattcacag atacggtaca attcatccaa aggaataatg tcttcgtggg cagcaatctg
     5641 caaaacaggg tcagctgaag tatagataat caactctcca gtttccatct gacgtggtcc
     5701 aaaatcatcg ataacagccg ttcctgaata aggtttgttg gcttcacgaa taaccttgcg
     5761 tcctgagaat tcttcgattt ttgtcaggat ttcttctggg aatccgttcc agaaagtatc
     5821 gaaaggctca gtaatgttga gtcccatgat ttcccagtgt ccagtcatag tatccttacc
     5881 aagagatact tcctctaatt ttgttgcata tccagttgga ttgctttcag ctgctacagt
     5941 cttaagagga gtttcacgag gaatatttcc aagacctatt ttagccatgt ttgggacatc
     6001 caaaccaact gtttttgaaa tgtgtcccag tgtgtcagaa gctccatctg gaacccctgc
     6061 attgacaaag ttattagcat ctggtgctgc accgattcct acagaatcca gtaccaccaa
     6121 atgaatacga ttaaatttag acatcttatg cccccttcta ttttcctttt tttgaagttg
     6181 aaatctgaac tccatctcgt ccagcaacga ttaccttatc caccatttgg ttgaataaac
     6241 catgctccac aacaccaacg acatggtcca attcttgtcc aaaagcaatt ggattttcaa
     6301 tgacatccaa ggcgaggtca atgataaaat tctgcatatc cgtcacaaaa cgttggccgt
     6361 ctttttcacg gaaacttggt ttgtagccag ctcgttcaaa atgacgaaag acctgctctg
     6421 caccatactg aaccacttct actggcaatt taaaagcacc tagtttttcg accagcttgc
     6481 tttcatccac cacccaaatg tattcttttg atggtgttgc gaccaccttt tccatgagaa
     6541 gggcaccacc accgcctttg attccattaa actgactatc cacttcatcc gccccgtcga
     6601 ctgtcacatc gacaaagtct acttggtcaa tagacttgag cgggatattg agcccttcag
     6661 cctgtttact ggtcacacta gaagtcgtca cagctgtaat ctgcaagcct tcttccttga
     6721 ttcgacgacc gatttcttcg acaaaataat aggcagtaga acctgttcct agccctacaa
     6781 ccatcccatc cttgataaat tcagcagccg tgatacctgc cattttcttc agattttcca
     6841 ctcaaacacc tccattaaag agctacctct attataccat gtaaaggctt ttatttcatc
     6901 taaaagtacc tttagaatac tgcttattta tccttgaaat tgaatttttt taataaaagt
     6961 agagctctaa caaatcaatt taagtacgga tgtaaaccaa atcaactcta acaaaaatag
     7021 atttacaaga gtaaaaaaac gaacaaattt agtttcttag caagaagaat actaaacgtt
     7081 cgttttttgt gaattaagaa taatgataca aattatcaac attttttgtc taactatttt
     7141 agacatatct tttcattact ttaggaagac aattactaat ttccgtcggc aagaccacat
     7201 aattttcttg agatagttct tgggctatgg ctgaatgaag atgggttgct actgccacac
     7261 gttcgtagag actggcctgt cgaaattggc ctgcaaatcc tgcaatcatt ccagccagtg
     7321 tatcacccat accaccagtc gcctgatagg gaccgccaac ctttaactgg taataatcag
     7381 actggccaac ttgccaaata cgagtagctg gacctttctc taccaaaatt gttccttgag
     7441 ggaaagaagt cagggcacta gatgttgtac cttcgttttg cttttcaata gcaataccag
     7501 acagtttttc ccattctttt tggtggggag ttaagataag ctggttagat ggaaacaaca
     7561 aacttgtcct agcaaggatg gttaaggccc ctccatctac aatcaaaatc tgattctttt
     7621 ttaagctagc aaagacctgt tttacaagat tttctccaaa cgtatcgtct cgtaaaccag
     7681 gccccagcaa gacaacttct gccttctcca attgctcttg taacaattgc tgatcttgca
     7741 gagaaaaggc catagcctca ggcaaatggc tgtgtagagc agggatattt tccctgtccg
     7801 ttccaacggt taccaatcct gcaccgcttt ttacagctgc taaagcagcc atgatgatgg
     7861 caccaccata aggataagtc ccaccaagca acagcagacg accgtagtct cctttatgac
     7921 ttgtacaaga acgttcaata atgacttttt ctagtaaggt ttgatcaatc actttcatcg
     7981 ttttttcctc aactatcctt tatctgattg ttttaaaaca acttgataga ctttcttagt
     8041 ccactcttct aagtcttctc cagcatagtc aagataatac accctatctt gtattttggt
     8101 tggaataata ttttcaaatt ggctcttatt atgagttgga ataatgcaaa tatacaactc
     8161 acttaataaa ttaagcatgt ccttaatttt tacagcatgg taaccatcaa aaatataacc
     8221 aggaacttta atcaaatctg tatagataaa atgaaaactt actccaggaa taaacttgtc
     8281 ttgtaattct ttctctgaag gagctcgacc taataaacgc tccatcgcca atcgataacc
     8341 agaacttgta tttgaccacc ctaacatgac atagtcaaaa taatctatcg gatcagatgc
     8401 agcatttcga ctatccaaaa ccaactcctc agcatctcgc ccaaaggctt tcacagcaga
     8461 taaaagacga ccagttttaa aaatagaaat cgctgcatct atagtcgtag tatgactaca
     8521 gtagtctgaa gtcactaacc tcttatgtag gtcataagtc gaggctactc ttttatctgt
     8581 aggagtaaat ggtggcagaa agggatgctg acagatgtaa tctaaaattt ctttttttaa
     8641 acaagaatca gcacactcaa tacttgtttg acgtttatga agtttttcgt aagctataaa
     8701 tttagcaatt ttttctaatt cccactcttg aatatttgga taatcaatta gtgctaagta
     8761 gtatattcca tcccattgta aactatattc cccattataa atttttaaaa tcatctaatc
     8821 acctcactcc attatacaac aaaaaggagg cgcaaacctc cttttataaa cttatttcta
     8881 aaatttaata ttcatttcta tcattttaga tatagctata gaaaatacac tttcctcaga
     8941 aaatttttat cttattttct atccaatgtc ctaagtgctg cctgataggt ttgctccatc
     9001 agcatagtaa tggtcatagg accgacacct ccagggactg gcgtaatgtg gctagcaagt
     9061 ggggcaaccg cctcataatc aacatcccca cagagcttac cattttcatc gcggttcatc
     9121 ccaacgtcaa tgactaccgc acctggtttg acaaagtcag cagtcacaaa cttggcacga
     9181 ccgattgcaa caaccagaat atctgctttt gcagccacct tggaaagatt atgagtacgt
     9241 gagtgagtca aggttactgt tgcattcttt gccaaaagaa gctgggccat aggttttccg
     9301 acaatattgg atcgaccgat gacgactgca tttttacctt ccaagtcaat cccatattca
     9361 tggaacattt ccataattcc tgccggtgtc gaaggaatca tgactggatg accagaccaa
     9421 agacgcccca tgtttagagg atggaaacca tccacatcct tttctgggtc aatagccaat
     9481 agaaccgcct cttcatcaat gtgttttggt aatggcaact gaaccaaaat cccatgccaa
     9541 gctggatcct gattgtattt agcaatcagg tctaacaatt cctcttgagt aatggtctct
     9601 ggaacccgta ctacttcgct acggaaacca gccgcaaggg ctgacctctc cttgttgcga
     9661 acgtagactt ggctggctgg attgtcccca accaaaatca ctaccaaacc aggcactaga
     9721 cctgtttctt cctttaattt tgcagtcttt tcagccaact gcccctgcaa tttggccgct
     9781 aaagctttcc catcaataat ctgtgtcata tcacttctct tttcttacaa atatctttaa
     9841 ttataccaaa aaatacaatg tagctctaac acttctttcc taggcaatcc tatagcttta
     9901 aactataaga aaaagctctg gttgagcttt tcactaatct tctctttaac gtttaaagct
     9961 ggctacaaat agctaaacaa agctaactta taaaacctta ctcaagaagt ctttagtcct
    10021 ttgtccttgg gtttgttcaa aaatctgctc aggtgttccg tcttcaacaa ccacaccgtc
    10081 tgccataaag atgacacgat ctgccacctc acgggcaaat cccatctcat gtgttacgat
    10141 aaccatggtc attcctgact tggctagatc ttgcataaca gccagaactt ctccaaccat
    10201 ctcaggatct agggctgaag ttggctcgtc aaagagcaaa acatctggtt ccatagccaa
    10261 cccacgcgcg atggcaatcc gctgttgctg gccacctgac aaactctgtg gataagcgtc
    10321 tgccttatct ggcaaaccaa ctttttccaa aagttcctga gctcttttct ctgcaacggc
    10381 cttactgtca cctttggtct tgatagggga caaggtgata ttttccatca cagtcatatt
    10441 aggaaagaga ttgaattgtt gaaaaaccat gcccatcttc tcacgcatgg caaacaggtc
    10501 attcttcttg tccgtaatat cgactccctc aaagataacc ttccccttgg ttgcttcttc
    10561 caacaaattc atagagcgaa gcaaggtaga tttcccgctc cctgaaggac cgatgataac
    10621 gacaacttct cctcttttaa tctcgaggtt gatgcccttc aatacttcat tctttccaaa
    10681 ggatttatgt aaattttcaa tttttatcaa ggtttctgtc attatttctt atctccttgt
    10741 cccatatgtt tttcaaaagc tttcaaggct actgtcagaa tagaggtcat aatcaagtag
    10801 taaaatgctg caaataaaag tggtgttaaa ggtagatagg ttgttgtaga aactgttgta
    10861 gccccattcc acaactccat gaccccaata gctgataaga gggagctgtc cttgataatg
    10921 gtgataaatt cgttccccaa tgctggcaag atatttttga ctgcttgtgg caaaatcaca
    10981 taacgcatcg catttttagg acgaatccct agcgaataag ccgcttctag ctgacctttt
    11041 ggaaccgcat tgattccggc acgaacagtc tccgaaacat aagcaccact attcatagag
    11101 ataatcaaaa tccctggaat cagacgcgaa aaatcaacac ctaaaattcc aatctgaata
    11161 gtcggagcat tgatatgcat aagagcaaag gcaatcataa tttgaaccat catcggtgtc
    11221 ccacggaaaa tccaaacgta caagttggcc aaccaaacaa gcggtttaaa ctttgaacgt
    11281 tgcccaaaag ccaagacaac acccaaaata gttcccaaaa agataacaca gatagaaata
    11341 agaatcgtca caacagcccc atagttaaaa taaggtaaat acttaggtaa aaaagaaaaa
    11401 ttcatagtca cactgctttc taaaatagaa tactgtatga ttataacatg ttttgaatta
    11461 aaatttaaat tttttatcca atttatttaa aaatatttaa actagcagaa ttagcgagaa
    11521 atcttagttt tttctagtca agttggcctc ctttatggta aaatagtaac gataagaaat
    11581 gaaggagaat caaaatggaa tcacatttgg ttagaatcat caaccgcctt gaagcaatgg
    11641 caaacgatgg cggaaattta aaacgtaatt ttgaacgcga aggagttgtt gttgcagaag
    11701 ttgcgtacag ccacgacgaa gaaaacggcc caatctttac ccttcgtgac gtagaagatc
    11761 gcgaaacgta tacgtttgat agcattgact taatcgcaat ggaaatttac gaacttctct
    11821 actaatctaa aacaagctgg gattgtccct gcatgtctaa gtaaaatcct ttttgtctca
    11881 caaataggat ttttttatag tccaaattca caaaaatttt cattgtaaca acttctcaaa
    11941 gcttcaatct ttttacttgc tttacacccc aatcctgtta taatgagagt atagaatttg
    12001 actatttttg acctttaaac aagtcaggct aaagaaagaa atgaggtaga tgtatgcttt
    12061 gtcaaaactg taaaattaac gactcaacaa ttcatcttta caccaatctc aatggaaaac
    12121 aaaaacaaat tgacctctgt caaaactgct ataagattat caaaacagat cctaacaata
    12181 gcctcttcaa aggtatgacg gatctgaaca atcgtgactt cgatcccttt ggtgatttct
    12241 tcaatgatct aaacaatttc agatcttcta gcaatactcc tcctattccc ccaacccaat
    12301 caggtggagg ttacggtgga aacggcggtt atggttccca aaatcgtgga tctgctcaaa
    12361 ctccgccacc tagccaagaa aaaggcctgc tggaagaatt tggtattaat gtaactgaaa
    12421 ttgcccgtcg tggagacatt gaccccgtta ttgggcgcga cgatgagatt atccgtgtca
    12481 tcgagattct caatcgtaga accaagaata atcctgtcct tatcggtgaa cctggtgtcg
    12541 gaaaaacggc cgttgtcgaa ggtctagctc agaaaattgt cgatggcgat gtgccacata
    12601 aactccaagg taaacaagtc atccgtctgg atgtggttag cttagttcaa ggaacgggga
    12661 ttcgaggaca atttgaagaa cgcatgcaaa aactcatgga agaaattcgc aaacgtgaag
    12721 acatcatcct ctttatcgat gaaatccatg aaattgttgg tgctggttct gcgagtgatg
    12781 gtaatatgga cgcaggaaat atcctcaagc cagcccttgc tcgtggagaa ctgcaactag
    12841 tcggtgctac taccctcaat gaataccgta tcattgaaaa ggatgctgcc ctcgagcgtc
    12901 gtatgcagcc tgttaaagtc gatgaaccaa cggtggacga aacaatcact attctcaaag
    12961 ggattcaaaa gaaatacgaa gattaccacc acgttcaata tacagatgct gcgattgaag
    13021 cagctgcaac tctttccaat cgctacatcc aagatcgctt cttgcctgac aaggccattg
    13081 acctcctaga tgaagctggt tctaagatga acttgacctt gaattttgtg gatcctaaag
    13141 taattgatca gcgcttgatt gaggctgaaa atctcaagtc tcaagctaca cgagaagaag
    13201 attttgagaa ggcggcctac ttccgcgacc agattgccaa gtataaggaa atgcaaaaga
    13261 aaaagatcac agaccaggat actcctagca tcagcgagaa aactattgag cacattatcg
    13321 agcagaaaac caatatccct gttggtgatt tgaaagagaa agaacaatct caactcatcc
    13381 atctagccga agatctcaag tctcatgtta ttggtcaaga tgatgcagtc gataagattg
    13441 ccaaggctat tcgccgtaat cgtgtcggac ttggtacccc taaccgccca atcggaagct
    13501 tcctcttcgt tgggccaact ggtgtcggta agacagaact ttccaaacaa ctggctatcg
    13561 aactttttgg ttctgctgat agtatgattc gctttgatat gagtgaatac atggaaaaac
    13621 atagtgtggc taagttggtc ggcgctcctc caggttatgt tggctatgat gaggctggtc
    13681 aattaactga aaaagttcgc cacaatccat attctctcat ccttctcgat gaagtggaaa
    13741 aagctcaccc agatgttatg cacatgtttc ttcaagtctt ggacgatggt cgtttgacag
    13801 acgggcaagg acgcaccgtt agcttcaagg atgccatcat tatcatgacc tcaaatgcag
    13861 gtacaggaaa gaccgaagct agcgttggat ttggtgctgc tagagaagga cgtaccaatt
    13921 ctgtcctcgg tgaactcggt aacttcttta gcccagagtt tatgaaccgt tttgatggca
    13981 ttatcgaatt taaggctctc agcaaggata acctccttca gattgtcgag ctcatgctag
    14041 cagatgttaa caagcgcctc tctagcaaca acattcgttt ggatgtaact gataaggtca
    14101 aggaaaagtt ggttgaccta ggttatgatc caaaaatggg agcacgccca cttcgtcgga
    14161 ctattcaaga ctatattgag gacacaatca ctgactacta ccttgaaaat ccaagcgaaa
    14221 aagatctcaa agcagttatg actagcaagg gaaacattca gattaaatct gccaaaaaag
    14281 ctgaagttaa aagttctgaa aaagaaaaat aaatcctata aaaaaggagt agaaaatgaa
    14341 atttttctgc ttcttttttt actaaaataa ctgtaatttc ttgacagctt gccctttgtc
    14401 cattatga
//
LOCUS       DAVHEX010000054        14384 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVHEX010000054 DAVHEX010000000
VERSION     DAVHEX010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14384
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.054"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(<1..1288)
                     /locus_tag="VVL45_001668"
     CDS             complement(<1..1288)
                     /locus_tag="VVL45_001668"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEU4187817.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIAMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQAHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVTLPIENALDVYIA
                     VLGEGANIKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(1366..2613)
                     /locus_tag="VVL45_001669"
     CDS             complement(1366..2613)
                     /locus_tag="VVL45_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEU4187818.1"
                     /translation="MKLLFRNPAYRILTLSRFFNAFGVSIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTLFTVFAGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDIISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVSVIISSLTGNDYFGKQSLPRLMMWETVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFLGLLFTLSIPVGTACFSLVAVWNIQLT
                     WMLFVGLSLLAIFLTILNLKNDI"
     gene            complement(2804..3654)
                     /gene="rgg"
                     /locus_tag="VVL45_001670"
                     /pseudo
     CDS             complement(2804..3654)
                     /gene="rgg"
                     /locus_tag="VVL45_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398326.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes.; frameshifted;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
     gene            3778..4119
                     /locus_tag="VVL45_001671"
     CDS             3778..4119
                     /locus_tag="VVL45_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU4187819.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            4177..4257
                     /locus_tag="VVL45_001672"
                     /pseudo
     CDS             4177..4257
                     /locus_tag="VVL45_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(4463..6166)
                     /gene="ilvD"
                     /locus_tag="VVL45_001673"
     CDS             complement(4463..6166)
                     /gene="ilvD"
                     /locus_tag="VVL45_001673"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEU4187820.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETPIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(6399..7331)
                     /locus_tag="VVL45_001674"
     CDS             complement(6399..7331)
                     /locus_tag="VVL45_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEU4187821.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSYSDIVVLEADLSSSMATNNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     SVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSSLCELFSDDGMTKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(7328..8185)
                     /locus_tag="VVL45_001675"
     CDS             complement(7328..8185)
                     /locus_tag="VVL45_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU4187822.1"
                     /translation="MILSKNREDGLRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEERQMLTSVVER
                     LSQELEETE"
     gene            complement(8189..9535)
                     /locus_tag="VVL45_001676"
     CDS             complement(8189..9535)
                     /locus_tag="VVL45_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU4187823.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATAMIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFVGGLVSMVIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSATEE"
     gene            complement(9548..9832)
                     /locus_tag="VVL45_001677"
     CDS             complement(9548..9832)
                     /locus_tag="VVL45_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU4187824.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(9836..11866)
                     /locus_tag="VVL45_001678"
     CDS             complement(9836..11866)
                     /locus_tag="VVL45_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEU4187825.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NSHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLIDQVVKDYYEKLQLVPIVNQLKVYLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKWYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
     gene            12058..13065
                     /locus_tag="VVL45_001679"
     CDS             12058..13065
                     /locus_tag="VVL45_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU4187826.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKQISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            13068..13268
                     /locus_tag="VVL45_001680"
     CDS             13068..13268
                     /locus_tag="VVL45_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU4187827.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            13420..13602
                     /gene="rpmF"
                     /locus_tag="VVL45_001681"
     CDS             13420..13602
                     /gene="rpmF"
                     /locus_tag="VVL45_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEU4187828.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            13618..13767
                     /gene="rpmG"
                     /locus_tag="VVL45_001682"
     CDS             13618..13767
                     /gene="rpmG"
                     /locus_tag="VVL45_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU4187829.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(14247..>14384)
                     /locus_tag="VVL45_001683"
     CDS             complement(14247..>14384)
                     /locus_tag="VVL45_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187830.1"
                     /translation="KSGSMATGWFTVSGKWYYTYNSGDLLVNTTTPDGYRVNANGEWV
                     G"
BASE COUNT         4642 a         3037 c         2486 g         4219 t
ORIGIN      
        1 attgagtata aaatcctagt ttttcaaaga tttctgagaa gttttggctg attgtctcaa
       61 gtgacacttg cacttcttct cgggtttggt tgttcttgac cgtcacttgt ccgctttcga
      121 cttcgctctc tcctagggtg atgagggtct tagccgcaaa gacatcggct gacttgaact
      181 gagcttttag tttacggttg aggtaatcac gctctgcttt gaaaccttgt tggcgaagag
      241 cctgtaccaa ttccaaggcc ttgatatttg ccccttcgcc caagactgcg atatagacat
      301 ctagggcgtt ttcgataggg agggtcacac cttgcttttc aaggatgaga agcaggcgct
      361 ctacaccaag tccaaaacca aatccagcag tttcagggcc tccaaagtaa gcaaccaaac
      421 catcgtagcg accacccgca cagacggtca ggtcattgcc ctcaatctct gtgataaact
      481 cgaaaatggt gtggttgtag tagtccagac cacgcaccat attggtatcg atgatgtaat
      541 ctactccaag attttccaac atctgacgca cagcatcaaa atgagcttgg ctttcttcat
      601 caagaaagtc caagatagac ggcgcattct ctactgccac cttgtcttct ttttccttag
      661 agtccaagac acgaagagga ttttcctcca agcgacgttg gctatcctta gacaaggtct
      721 ccttgagcgg tgtcaaatag tcaatcaagg cttggcggta ggctgcacgg ctctcaggat
      781 ttccaagagt gttgaggtgc aatttgacac cttgaatacc gatttccttc aaaaaatggg
      841 ctgccatagc gattgtttcc acatcggtag ctggattgct agagccaaaa cactcaacac
      901 caatctggtg gaattggcgc aagcgccctg cctgtggacg ctcataacgg aacataggtc
      961 ccatgtagta gaacttgctt ggcttttgca cttctggggc gaaaagttta ttttccacat
     1021 aggaacggac aacgggtgca gttccttctg gacggagggt aatatgacgg tcacccttgt
     1081 cataaaaatc gtacatttcc ttggttacga tatccgttgt atctccgaca gagcgactga
     1141 taacctcgta atgctcaaaa ataggcgtgc gcacttctgc atagttgtag cgtttgaaaa
     1201 tctcacgggc aaagccctca acgtactgcc acttagcaga ctcagcaggt aaaatatcct
     1261 gcgttccttt tggtttttgt aatttcatag ggaatcctct ttaaacttaa tagtcttatt
     1321 ttaccataaa tagagggatt aaaacagtaa gaaaaaaatt aggatttaga tatcattttt
     1381 gagattaaga attgtcaaaa aaatagctag caaggaaaga ccaacaaata gcatccaagt
     1441 caactgtata ttccatacgg ctactagtga aaaacaagct gttcccacag gtatggataa
     1501 ggtaaacaat agacctaaaa aattactagt acgagctaga acctctggag ctagattttt
     1561 catgagcatg gcactaatct ttggttgaac tttaccagac acatacagag taaagaagag
     1621 aaatagcaaa ccaagcacga cttgattgaa taaattagcc aaaccaacta gactaagtcc
     1681 tacggtctcc cacatcatca atctaggcaa ggactgcttc ccaaaataat cattgcccgt
     1741 aaggctactg atgatgactg atactaaaac acagaattga ttgataaata gtgcctctgt
     1801 ataagaaaaa ttcaagagag aatggctcaa aaagaagata ttataaattc cacccaaagc
     1861 gccacccaag gaattaataa gcaagacagc aaagagcata aaaccaaagt ttttctgtcc
     1921 acttttaaga aaaacgagac gtaaatttcg gtaaattgtt aggaactggt ctttgataga
     1981 aagcttctca ttttttaagt tttcaccatc agcagatgac attgacaggc tcaatttgct
     2041 ttttcctaaa aagaggatag tggctgatac taggaaaaag caggcattga ttcccgcaac
     2101 gagagaaaaa ttgttgaccg atagagctaa gagccagact ccgaaagctt gaccaccaat
     2161 agctgaaata taggtgatga actgtgaaaa agaataagcc tccatcagat catcttcagc
     2221 tactttttcc ttaataagag gcatacgcag gccacctgca aaatcactga tgatatcact
     2281 aatgacattg atcaaacaca ggctagaaaa ggcaaagaga ctagcttgct gaacaactag
     2341 ggctgctaga aaaaatagaa ccgcctgaaa caaaccgcta tagaccatcc atttgacctt
     2401 gtccctcgtg taatctgccc gaatccctgc aaaaactgta aagagggtcg gaagaatcat
     2461 gacaatattc gccatagcaa cagcaaaaga tgcttgtgac aaggtcgatg catagacgat
     2521 aaagaccagg ttgaaaatcg aaacaccaaa agcattgaag aagcgtgata aagtcaaaat
     2581 tctataagct ggatttctaa acaatagttt catagataat ctccctcctt gttctaagtt
     2641 ctacatcaag aatagcttta gtatagcact tatgaatttg cgaaacgatt cttttgaata
     2701 tatgcaacaa acaaaatgag caaaaaaacg atagaacttt tgtaatgacc tgagagaggg
     2761 caacagttct atcgcttcaa attttttctt ggtttgcaga tattcaagaa tcgggagttt
     2821 ttctatagta ttcggcagat ttattacagc caagcatctc aaaaatacgg acagcatcct
     2881 ccatcttttt ctggccttcc ttgactctac cttgcttgct atcaaggaga ccttctgccc
     2941 acagataaac aattcggaaa taggtctcat tttccttgta gaaatgctct tcgataacac
     3001 gtttaaaata ataggcattg gtaaattctt cacactcaat actagctaaa aagccattca
     3061 atagtatagt atgaaaaagg tttcgattgc cagacatttc cattagaaaa tcagatttac
     3121 gtaccatttc tcgtacatat ctagtaaaaa gagaaacaga taaaaatgga gaactgactg
     3181 aaaataaatt gagttcatag attccccaga tctcggtaga aaacaaataa tcatgaagga
     3241 cttttccttc ctctgctgtt aagtctaccc tttcatctat gctctttata taagacttga
     3301 taataatggc atttagaata tgtttctgtt tgttgtgaga atgggcatgc ttttatactc
     3361 cctgcgatat aagtcctcaa gaggtgctat attctttggt tccaagacat ctgtaatttc
     3421 ttttctcaac tcagaatctg tatcatactg gaaacctctt gccagaaaga ggatctcctc
     3481 cacactggca gatatatttt ccagagcaaa tagaaacttt tccaccgaaa gctcactctg
     3541 acctgtttca aaacgggaca acatagacgg cgaaaattgt cctccggttg cttgtctcag
     3601 tgagatattt cttgactctc gtaattgtct aaagactttt ccaatctgct ccatagactt
     3661 ccccttgatt ccgtattttc ttcattttat catatttttc agaaaattca tcaaaaactt
     3721 gccaaattgt cagaattatg agaaaataga ggatatttat cacgtggagg gactgctatg
     3781 agagacgata tcaaaatcaa tgaccgtgct ttggccttgc aagaccaaat tatcgaaaaa
     3841 ctagagaaag tttttgatac agatgtggaa ttggatgttt acaatctagg tctgatttat
     3901 gaaatcaatc tggatgaaac ggggctctgc aagattgtca tgaccttcac cgatactgcc
     3961 tgtgattgcg ccgaaagcct gcctattgaa atcgtggcag gtctgaaaca aatcgagggt
     4021 atcaaagata tcaaggttga agttacctgg tcgcctgctt ggaaaatcac acgaatcagt
     4081 cgctatggcc gtattgccct tggactacca cctcgttaag cagaccaatc acttttaaag
     4141 atgaaaaaag caagccgaaa atggcttgct ttttgatact caatgaaaat caaagggcaa
     4201 actagaaaac tagccgcagg ttgcccaaaa cactgttttg aggttgtgga tagaactgac
     4261 gaagtcagcc caaaacactg ttttgaggtt gtagatagaa ctgacgaagt cagctcaaaa
     4321 cactgttttg aagttatgga tagaactgac gaagtcagct caaaacactg ttttgaggtt
     4381 gtagatagaa ctgacgaagt cagtaaccat acctacggca aggcgacgtt gacgtgattt
     4441 gaagagattt tcgagtatga gtttattttt tacctgactt gtccatattc cagaagtctg
     4501 tcacggctcc gcgtgaagca gatgatacga tgtgggcata tttaccgagg acaccacggc
     4561 tgtaaagtgg tggcaaggtt gtttctgcct tgcgtttttc aagttcttct tcggatacgg
     4621 ccatggaaat ttctttggta tcttggtcaa ccgtaacgat atcgccggta cggagatagg
     4681 caattggtcc accatcctga gcttcaggag cgatatgtcc aacaaccaga ccataagtac
     4741 caccagagaa acgtccgtcc gtcaagaggg ccaccttatc tccctgacct ttaccaacaa
     4801 tcattgaaga aagtgacagc atctcaggca taccaggacc acctttaggt ccaacaaaac
     4861 gaacaacgac tacatcgcca tcaacgattt catctgtcag aacggcctga atcgcatctt
     4921 cttctgagtc aaagacctta gctggcccaa cgtgacgacg cactttaaca cctgatacct
     4981 tggcaactgc accgtcagga gcaaggttcc cgttcaagat gataagcgga ccatccgcac
     5041 gttttggatt ttcaagtggc atgataactt tttggcctgg agtcaagtct gcaaagtcag
     5101 ccaagttttc agctacagtc ttaccagtac atgtgatgcg atctccgtga aggaaaccat
     5161 ttgccaacaa gtacttcata accgcaggga caccaccgac ttcgtagagg tcttggaaga
     5221 catactgacc agatggtttc aagtcggcca agtgaggcac acgttcttga atcgtattga
     5281 agtcctcaag tgacaagtca acatttgcgg catgggcaat ggcgagcaag tgaagagtgg
     5341 cgtttgtaga accaccgaga gccatcgtta cagtaatagc atcttcaaag gcttcacgag
     5401 tcaagatatc tgatggtttg agaccaagtt ccaacatctt aacaacagca cgtcctgctg
     5461 cttcgatatc ttctttctta tcagctgatt cagctgggtg agaggatgac cctggcaaac
     5521 tcatccctag aacttcgata gcagttgcca tggtattagc agtatacata ccaccacagc
     5581 caccagggcc agggcaggca ttacattcaa gacgtttcac gtcctcagct gtcatgtcac
     5641 cgtggttcca ttttccgata ccttcaaaga cagaaaccaa gtcgatatct ttaccatcaa
     5701 gatttcccgg tgcaatagtt ccaccatagg cgaaaatagc tgggatatcc atattagcaa
     5761 tagcaatcat agatccaggc atgttcttgt cacagccacc gatagcgaca aaggcatcca
     5821 cgttgtgacc actcatagcc gcctcgatgg agtccgcgat gatgtcacga gatgttagag
     5881 agaaacgcat accaggcgtt cccatagcga tcccgtctgc tacggtaatg gttccaaact
     5941 gtacaggcca agcgcctgca gatttgacac cttctttagc cagtttcccg aaatcatgca
     6001 agtgaatgtt acatggtgta ttttccgccc aagtcgaaat cactcccaca atcggtgttt
     6061 caaagtcctt atctgtcata ccagtcgcac gaagcatagc acggttaggt gatttaacca
     6121 tgctgtcata aatgctactg cggtgacgtt tatctaattc agtcatctta tccctcccat
     6181 ttcagttttt actattatag cacaattttc gcatgaagaa cagaataaaa atcttgaatt
     6241 ttcagaaaat tctatacaca tgtgaaatat ttaaaattaa aaacaacaaa gcggattagt
     6301 gcactttctg atgaccagaa tatgcttttt aatccgcttt ctttaaataa cgtactgtaa
     6361 tttttacaga aattctttca gataagtgta tttaacatct atcttgcatt ataaatttct
     6421 agaaccttct cttttatatt cgattcactc aaaccatact cattaagaag ataatccatt
     6481 ttccctactt gaccgaatct ttcttgaaca cccatccgat gaatttttgt cattccatca
     6541 tcagagaata attcacataa agaactgcca attccaccta tctgattgtg gttttctaca
     6601 gtaaatatag tttttccact taacattgtt tttatctgtt ctggtatcgg tttgattcta
     6661 aataaatcta tcacacttac tgaataacct aatttagaca gttcatctgc aactcgaata
     6721 cttggagcaa ccattatacc agaagcaacg attacaagat cttcaccatg ccttaactca
     6781 atgtagcctt tagaaaaatc ttctccacct tgatacacag gaactggagc ttttctaatt
     6841 gttcgaatat attttagtcc ttttaagtct aatgtctggt tcaatatttc acgaaattgg
     6901 atatcatcag ttgcttcaaa aatgatcgat ttaggaatta aacgtaacaa tccaatttct
     6961 tcaaatggca tatgtgtccc accattcatt tctgcagtta ctcctgcatc tgatccaatc
     7021 acagtggcat ccaattgtgc gtatccaaga gaaataaata attgatcaaa tactcttcgt
     7081 gaagcaaaag gaccaaatgt atgaagataa ggtctaaacc cctgaataga caagcctgct
     7141 gcaaggccga ccatttctgc ttccataatc ccaacattca cataacggtc tccaaaatcc
     7201 ttttcaagat tattagtagc catcgaactt gacaaatcgg cttctaagac tactatatca
     7261 gaataacttt gattagcctc taaaaggaag tctctatata catgccgtaa ttctttcgtg
     7321 cttctcatca ttctgtttcc tccaattcct gacttaatct ttctacaact gaagttaaca
     7381 tttgtctctc ctctacagta gggcgaagat gatggttgga tttcatttct tccagctctt
     7441 gaaccccttg acctttaata gtatctaata caatgcactt aggtgatgaa ttatttgact
     7501 gttttaattg gacaatccct tcataaattt ctctaatatc tgaacccttg accctaatgg
     7561 attcaaatcc aaatgctgaa aatttttcta cgaaatcacc tggattacaa atatcctttg
     7621 taaaaccatc taattgtttt ttgttatcat caacaaatac aattaagttg gataactgtt
     7681 gatgagaagc aaactgtata gcctcccaac attgtccctc atttaactca ccatctccaa
     7741 caatagcgta agtataaaag ggactctttc ttattctctg accatatgca agtccagttg
     7801 caacactaat tccttgtcct aaagagcccg ttgtcatatc tatgcctggc gttagatttc
     7861 tatcaggatg agacggtaat ttggttccat ttgtatttaa agaatataag aattctttgt
     7921 caaagaaacc attcaaatag agtgtactgt atagagctgg tcctccgtga ccttttgata
     7981 atatgaaata atctctatct cgtgctgcaa atatttctgg agtcattggc attatttcac
     8041 cataaagcac cgctaaaact tctacgatag acagacttcc tccgtaatgt ccgaatccaa
     8101 gatgattcaa tgttctaaga gtatttaatc ggatgttagt cgcaaatttt cttaacccat
     8161 cttctctatt tttacttaaa atcatccctt attcctccgt tgcagatggc tttttaataa
     8221 aggatactcc aaacataact gctagaataa gaacaagacc aatcacaatg cctgcttgtg
     8281 agccaaattg atttaacatt cctaaaataa ttcctgatag accaaaatct gcatctgaga
     8341 aagttgatcc ttggaaacca agtcctccca aaactggcat taaaaagact ggaagaaaac
     8401 tgattaaaat accttgtaaa aatgctccaa tagtggctcc acgaacacca ccagatgcat
     8461 tcccaatgac acctgcagtc gctccacaga agaaatgagg cacaacacct ggtaagataa
     8521 caaccgttcc tgaagcaatc ataattacca tacttactaa accaccaaca aaactagaga
     8581 taaatccaat tagaactgca ttgggtgcat aagtataaac aatcggacaa tccaaagcag
     8641 gttttgaatt aggtacaaga cgctctgaaa tacctttaaa ggctggaaca atttcgccca
     8701 aaataaggcg aacacctgct aaaataacaa atacccctgc tgcaaattga cctgctaatt
     8761 gtaaagcata aactagacca cttgtaccac tactgatttc tttttctata tattctgacc
     8821 ctgcaaagat agctacaata atgtaaataa ctgccatgga taaagtaata ctaacagtac
     8881 tatcacgtaa aaaagctaaa ctctttggaa atttaatgtc ctctgttgat tttgatttgt
     8941 caccgataag gctaccagta aaaccactca accaatatcc caaagaactg aaatgaccta
     9001 aagctacctt gtcatttcca gttaattgaa ccatatattt ttgcacaaat gctggggaaa
     9061 tactcataat aataccgagt gctaatcctc ctagtaagat gagaggcaag ctagtaaagc
     9121 cagcaactga taaaatgacc gcaatcatac atgccatata tagagtgtgg tgccctgtta
     9181 aaaaaatata tttaaatcga gtaaaacgag cgattaagat attgaacacc atgcctgcaa
     9241 acataatcat tgcagtagct gagccatatg ttgttaaagc tacagctaca attgcttcat
     9301 tattcggcac aacgccagat aaatgaaaag catgctcaaa catggtacca aatggattta
     9361 aagaattttg tacaattcct gcaccaccag atacaactaa gaaaccaaca aaggtcttaa
     9421 ttccaccttt aataatatca ggtaatttct tcttctgaag aactaatcct aagattgcaa
     9481 ttaaagctac taaaatagct ggtgtactaa caatatccaa tatgaacttc atcatgacgc
     9541 tagcctccta tataagtcct ttttcttcac aaagtttagt aattaattct cgtagttcat
     9601 ccatatcaat aatactattt aagatacgaa catctccaag atgactagct gaatcagcta
     9661 gatcacgacc aacaatccaa atatcagctg catttggatc tgctccacct aaatcataat
     9721 gttcaacttc tacatccgaa acattcaaat cactcaatac agattcaata ttcatctgta
     9781 ccataaaact tgaacctaat cctgaaccac aagctgtacc aatttttaac attatctaat
     9841 cctcctgttt aattatcatt ttaatgtcat catagttttt tgatgatatt aaagtttgaa
     9901 catgattttt atctcttaaa attgttgtta aatgtgacaa agcctttaaa tgactctcat
     9961 tatcaatggc tgcaatacaa atcaacaatc ttacctcttg ttctggatta tccaataaat
    10021 aaatcggttc ttccaaaact aacattgaca ttcctatttc attcacacct tcatctggcc
    10081 gagcgtgagg aattgctact cccttcccta aattaataaa aggcccaaac tcttctactt
    10141 tttgaatcat tgcctcaggg tagttctcag ttatcttatc ttgatccaaa agcggtttag
    10201 ctgctaaacg aatcgcctcc ttccatccta atttttgcga actaacctga taggtttctt
    10261 tggtaatgag ttgttctagc actggtacaa tttcctttct atcatttttt tggtaaagat
    10321 aattctttaa cgccaatctt aattccaatt cttgtgtaat aattccatat cttttgacaa
    10381 tattcaggat ttgttcaatc tcaaaatccc catactctaa attcggaaaa tcttttaaca
    10441 ctaattctac tagttgtatt gcttgctctt cagtcatcat aaccgaaact agataatttg
    10501 gcttttctgt ctccaccttt atggtagaaa aaaccatatc atagtcacta ctagctttca
    10561 cctgtaaatc atcaatcttt gaggttccta taaactcaat ttgaggaaat aatgctaata
    10621 gattctcttt taacatcaat gaagaactaa caccattagg acaaatgatt gctgctttat
    10681 accatttttg aggcaaagta tctgctttct ttaaataacc tccgaaatgg ataacaaaat
    10741 atgctgtttc actatcaggt atgggattgt caatagcgtc catcaagggc atcaaagaat
    10801 ctttgactaa ttcaaataaa tcaggataat gttctttaac atgcaataca tattcatttg
    10861 aactaggtag gccgaacttt aatctatagt aagccggtat aaggtggcgg cgaagatttt
    10921 ctctcaatcc ttccctttgt ttaaaatgta acaaagaaat atcttccatt ctacttataa
    10981 tagcctctgt taattgatta aagtaaaccg gagcaacatc tacttcacct tcaaagcaac
    11041 ttgataataa aacggtgata tagcgataat catcctcaga aaacaccgta tctataattc
    11101 ccaaatcaac cactgtatcc aataaaatag tggttatatc ttgaataaca ggagatacta
    11161 atgtctctga aagacatact ctttcaacat ccctttgata cctacacaga atgaatacta
    11221 aaccgaaaag gtaaactttt aattgattaa caataggtac tagctgtagc ttctcataat
    11281 aatctttaac tacctgatca atcaaatcat aagttaatga atacccccaa ctggataaaa
    11341 cataatccaa accccaaatc cctatggagg attccagcaa ctcactaacc atttgaaaag
    11401 ctaagcggtg cttattccac tctgaaccgt gtaaagtata accttttgct ctactgtacc
    11461 ctagctccaa atcattatct aacataatct ttcttaatga ttgaatatca gataaggttg
    11521 tattcttact tactttcaaa aagtcttggt aatgactatt cgatataaaa tctaatcggc
    11581 aaaaagtgta aagatagatt aaagctaagc gagtcgactt tggtaaaacc aattcatccg
    11641 acttaataat atctgtcaaa gactgcttcg tacgatttga taaactatag cgaccttgct
    11701 ttttatccag cactatccct ttattagcta gataaggcac taaataatct attccttctt
    11761 tgacttcctt tataggtaag ctcaccttaa cagataattc atataatgat agctcacaat
    11821 gatccatcaa agtcatcaaa ataactagtg ctctataatc aaacatttct cttcctttct
    11881 aactacaatt atagtgtaaa aattataaga ataaaagagt atttgagcac aatataatct
    11941 caccttttaa aagaaattat tgggctgaaa gtttttagtg attatttttc aactttctat
    12001 tgactttata aattaaagtg atatcattta gatatcaaat aacaggaggt cattcttatg
    12061 actgaaaaac taatcaattc aaaaccaaat ggtgtattcg cattgattct cattgagttg
    12121 acaatcgtac ttggtatctt tatatttata atgggggttg gttcggaaaa catttttgga
    12181 attattatcg gacctttact aatcgtaatt gcaggtctag ctcatgctgg tttaaaagtt
    12241 gtcaaacctc aagaagctct ggttctgaca ctctttggta actatacagg taccatcaaa
    12301 gaacctggct tttactttgt caatcccttc agcgtagcag tcaaccctgc aaaccacact
    12361 cgacttggac aaagtggtga tgttagcaca aaatctcctt ttttaggagc taaatcatca
    12421 aatgacaatg atgtaaatct tgaaattggc aagaaacaga tttccctcaa agtcatgacc
    12481 ttgagcaatt ctcgtcaaaa aatcaatgat tgcttaggaa accctgtaga aatcggtatc
    12541 gcggtaactt ggagagttgt cgataccgct aaggcagtct tcaacgttga taactacaaa
    12601 gaatatcttt cattgcaatg tgatagcgcc ctccgtaata ttgtccgcat ctatccttac
    12661 gatgtgtctc ctaatgtgga tactacgggt gatgggcaag cagatgaagg tagtctccgt
    12721 ggctctagcg aaattgttgc taaccgtatt cgtgaagaaa tccaaagtcg tgttgaggat
    12781 gctggcttgg aaatccttga agcacgtatc acttacctag cttatgctcc agaaattgct
    12841 gccgttatgc ttcaacgcca acaagcatct gccattattg atgcacggaa gatgattgta
    12901 gatggtgctg taggaatggt tgaaatggca ctagaacggc tcaatgaagg ggaattggta
    12961 gaacttgacg aagaacgaaa agctgccatg gtttcaaatc tccttgtcgt tctttgtggc
    13021 aatcatgatg cacaaccaat tgtcaacaca ggaagtcttt actaaagatg gctgaagcta
    13081 aaaaaaagca gatccccctt cgactctcaa caaagttata cgctgcactc gcatcatggg
    13141 cagaagacga ctttcgttca gtcaatgggc aaatcgaata tctcctcaca gagtgtgtca
    13201 agcaacggaa gaaagacgga aaatacgtat cagaaaccat tgacgaacca tttgagattg
    13261 atatttaaca ccttctcctg tctgccgtgt cggataggag aattttttca tctttttttg
    13321 tcaagtaact ttactttaca aaaaaaatgt gttatcctag tatggttgat gaaaatcagt
    13381 agattgaatc gaatataaat acctaaagga gaaatcaaaa tggcagtacc tgcacgtcgc
    13441 acttcaaaag cgaagaaaaa caaacgtcgt acacactaca aagtaacagc tccatctgta
    13501 aactttgacg aaactactgg agattactca cgttctcacc gtgtatcact taaaggatac
    13561 tacaaaggac gtaaaatcgc taaagctgca tcagctgaat aatagaaggg agataccatg
    13621 cgcgtaaata ttacacttga acacaaagaa tctggtgaac gcttgtacct tacttctaaa
    13681 aacaaacgta acactccaga ccgccttcaa ttgaagaaat actcaccaaa acttcgcaaa
    13741 cacgttgtgt ttacagaagt gaagtaagca ttcaatacga atcaatacag aagaaaaacg
    13801 cttgatcagc gttttttctt tttgttaatt tcactatatt gcaagggaaa tcattcgaat
    13861 gactaccttt tttgaaataa gaatacggat tcggacaatt aaaagcaaga ggaactcaaa
    13921 tgtaagttgg ggttcctttt ttgtatattt ttcaaataac gcctctacac tatttgtagc
    13981 aaattcacca actacagttg tatcttagtt aaaataagtt ataatatgta agtgagtacc
    14041 agatatacca agacatcgtc accatctaag gtatattcaa aatacaaaag ttgaccaact
    14101 agatttctga atatccttat atatccattc ttaaaattgg tttaaatagc gtagtctttt
    14161 aaactagttt tgagaatcca aaaaatcttc ctacatatgt aagaagattt tttagttcag
    14221 aatgattaga tttagctaat ggatacctat cctacccact caccgttagc attgactcga
    14281 tagccatcgg gtgtagtcgt gtttactaat aaatctcctg aattataggt atagtaccat
    14341 ttaccagaaa ctgtaaacca accagtagcc attgaacctg attt
//
LOCUS       DAVHEX010000055        14251 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVHEX010000055 DAVHEX010000000
VERSION     DAVHEX010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14251
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.055"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            130..927
                     /locus_tag="VVL45_001684"
     CDS             130..927
                     /locus_tag="VVL45_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEU4187831.1"
                     /translation="MVSSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            1075..1197
                     /locus_tag="VVL45_001685"
     CDS             1075..1197
                     /locus_tag="VVL45_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine sulfhydrylase"
                     /protein_id="HEU4187832.1"
                     /translation="MTCDFKFETLQLHAGQVVAPATKSRAVPIYQTTFFVFDDT"
     gene            1219..2259
                     /locus_tag="VVL45_001686"
     CDS             1219..2259
                     /locus_tag="VVL45_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HEU4187833.1"
                     /translation="MRKPGNIYTRITNPTTAALEGGVEALATASGMTAVTYTILAIAH
                     AGDHVVAASTIYGGTFNLLKEPLPRYGITTTFFDIDNLEEVEAAIKDNTKLVLIETLG
                     NPLINIPDLEKLAEIAHKHQIPLVSDNTFATPYLINVFSHGVDIAIHSVTKFIGGHGT
                     TIGGIIVDSGRFDWTASGKFPQFVDEGPSCHNLSYTRDVGAAAFIIAVRVQLLRDTGA
                     ALSPFNAFLLLQRLETSSLRVERHVQNAETIVDFLVNHPKVEKVNYPKLADSPYHALA
                     EKYLPKGVGSIFTFHVKGGEEEARKVIDNLEIFSDLANAADAKSLVVHPATTTHGQLS
                     EKDLEAAGVTPN"
     gene            2430..3704
                     /locus_tag="VVL45_001687"
     CDS             2430..3704
                     /locus_tag="VVL45_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEU4187834.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKKLHDRMKQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHEALLAKDKEV
                     QLLENQLATLRLEHENQLQKTLSDLEKERDQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKNFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            3714..4592
                     /gene="truB"
                     /locus_tag="VVL45_001688"
     CDS             3714..4592
                     /gene="truB"
                     /locus_tag="VVL45_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEU4187835.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEITLGYSTKTEDASGEVVAETPVLSLLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALALEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLSPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            4833..5135
                     /locus_tag="VVL45_001689"
     CDS             4833..5135
                     /locus_tag="VVL45_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEU4187836.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQS
                     VMANLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            5243..5881
                     /gene="udk"
                     /locus_tag="VVL45_001690"
     CDS             5243..5881
                     /gene="udk"
                     /locus_tag="VVL45_001690"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEU4187837.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            6009..7349
                     /locus_tag="VVL45_001691"
     CDS             6009..7349
                     /locus_tag="VVL45_001691"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEU4187838.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLAHLQNQEK
                     RMVTAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESVVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            7327..7539
                     /locus_tag="VVL45_001692"
     CDS             7327..7539
                     /locus_tag="VVL45_001692"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEU4187839.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            7536..8411
                     /locus_tag="VVL45_001693"
     CDS             7536..8411
                     /locus_tag="VVL45_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEU4187840.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVTIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDSYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            8404..9219
                     /locus_tag="VVL45_001694"
     CDS             8404..9219
                     /locus_tag="VVL45_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEU4187841.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            9212..9643
                     /locus_tag="VVL45_001695"
     CDS             9212..9643
                     /locus_tag="VVL45_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU4187842.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIDTFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            9650..11317
                     /gene="recN"
                     /locus_tag="VVL45_001696"
     CDS             9650..11317
                     /gene="recN"
                     /locus_tag="VVL45_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEU4187843.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNEMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            11319..12047
                     /locus_tag="VVL45_001697"
     CDS             11319..12047
                     /locus_tag="VVL45_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEU4187844.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQPQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYRRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEASDLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            12126..13949
                     /gene="lepA"
                     /locus_tag="VVL45_001698"
     CDS             12126..13949
                     /gene="lepA"
                     /locus_tag="VVL45_001698"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEU4187845.1"
                     /translation="MNLEELKKRQRKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
     gene            complement(14030..>14251)
                     /locus_tag="VVL45_001699"
     CDS             complement(14030..>14251)
                     /locus_tag="VVL45_001699"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187846.1"
                     /translation="RKLAAGYSKHSFEVVDKTDEVSSKHDFEVVDETDEVSSKHGSEV
                     VDETDEVSNHTYGKATLTWFEEIFEEYKN"
BASE COUNT         4205 a         2608 c         3385 g         4053 t
ORIGIN      
        1 atgttttaga agtagaggtg tactattcta gtttcaatct actataaact tttatgaaat
       61 acagaaaacg cttcctaaaa cctaaagttt gtgatataat tatcaagaaa aaagatttag
      121 gagtttatta tggtttcttc ggaatttatc tcaaagattg aatttgcttg caataagaaa
      181 gaaagtcttt atagtcaaag caaatttaag tatgcgattc gttcgatgtt cgcaggtgca
      241 tttttaacct tcagtactgc tgcaggtgca gttggggctg acttgattaa taaaattgca
      301 ccaggtagtg gacgcttcct ctttccattc gtttttgctt ggggcttggc ctacattgtt
      361 tttttgaatg ccgagttggt cacttcaaac atgatgttct tgactgctgg tagtttctta
      421 aaaaaaatct cttggagaaa aacagctgag attttactat actgtacctt gttcaacctt
      481 atcggagcct tgatagcagg gtggggcttt gctcattcgg cagcctatgc gaatctgaca
      541 cacgatagtt tcatctcagg tgttgttgag atgaagttag gccgctcaaa tgaattggtc
      601 ttgcttgagg cgattttggc aaatattttt gtaaatattg cgattctgtc atttattttg
      661 gtcaaagatg gtggtgccaa actttggctt gtgttgtcag ctatttacat gtttgtattc
      721 ttaacaaacg agcacattgc ggcgaacttt gcttctttcg cgattgtgaa attcagtgtt
      781 gctgcggatt caattgccaa cttcggtgtt ggaaatatgc ttcgccactg gggtgtgact
      841 ttcatcggaa actttatcgg aggaggcctc ttgatgggtc ttccatatgc cttcctcaat
      901 aaaaacgaag atacttatgt agattaagaa aatgagcacg attgagtcgt gcttttttca
      961 ttttcaaaat aaggtaatag ctatttctta tatcaaaata tagaaaactg atatttgtaa
     1021 actataactc aaggtgctac aatatcctta ataaaataat atggaggtca ccttatgact
     1081 tgtgatttta aatttgaaac tctacaacta catgctggtc aagttgtggc tccagctact
     1141 aagtctcgtg cagtgccgat ttatcaaaca acattttttg tttttgatga cacgtaggaa
     1201 ggtgccgatc tgtttgcctt gaggaaacca gggaacattt atactcgtat caccaatcct
     1261 acaacagctg cccttgaagg tggtgttgaa gcgctagcaa cagcatcagg tatgactgca
     1321 gtgacttata cgattttggc gattgcccat gctggtgacc atgtagtggc tgcttcgact
     1381 atttacggtg gaaccttcaa tcttttgaaa gaaccccttc ctcgttatgg tatcacaaca
     1441 acctttttcg atattgataa tttggaggaa gtagaagcag ctatcaaaga caataccaag
     1501 cttgtcttga ttgaaacctt gggtaacccc ttgattaata ttccagacct ggaaaaactg
     1561 gcagagattg ctcataaaca tcaaatccca cttgtgtcag acaatacttt tgcaacacct
     1621 tatttgatta acgtcttctc tcatggcgtt gacattgcca ttcactctgt gactaagttt
     1681 atcggtgggc atggtacaac tattggagga ataattgtcg atagtggtcg ttttgactgg
     1741 acggcttcag ggaaattccc tcaatttgtt gacgagggtc caagctgcca caatttgagc
     1801 tatactcgtg atgtgggtgc agcagccttt attatagctg ttcgagttca attgcttcgt
     1861 gatacaggtg cagccttgtc accattcaat gctttcctct tgctacaaag acttgaaacc
     1921 tcttcacttc gtgtggaacg ccatgtacaa aatgctgaga caattgttga ttttcttgtc
     1981 aaccatccta aggtagaaaa ggtaaattat ccaaaacttg cagatagtcc ttatcatgcc
     2041 ttggctgaga aatacttgcc aaaaggtgtc ggttcaatct ttaccttcca cgtcaaaggt
     2101 ggcgaggaag aagcacgcaa ggtcattgat aatttagaaa tcttttctga ccttgcaaac
     2161 gcggcagatg ctaaatcgct tgttgtccat ccagcaacaa ccactcacgg tcaattgtca
     2221 gaaaaagacc tagaagcagc aggtgtcaca ccaaactaaa ttcgtttgtc aatcggtctt
     2281 gaaaatgtag aagatttgat tgaagacttg cgcttggcct tggaaaaaat ttaaagtaaa
     2341 agaagataaa cagtgggctt cgactcactg tttttgattt tccctcaggc atgatataat
     2401 ggttacagaa gtctagaaag aggaacgata tgaacgaaat caaatgtccc aactgtgggg
     2461 aagtctttac agtaaatgag agtcagtatg ccgaactctt gtcccaagtg agaacggcag
     2521 agtttgataa gaaactacac gataggatga agcaggaact ggccttggct gagcaaaagg
     2581 ccatgaatga gcaacagact aaactggctc agaaggatca agaaattgcg caattacaga
     2641 gtcagatcca aaactttgat acagaaaaag aattggccaa gaaagaggtt gaacagacaa
     2701 gccatgaggc tctcttggct aaggacaagg aagtacagct cttagaaaat cagttggcta
     2761 ccttgcgttt ggagcatgaa aatcaactac aaaagaccct ttctgaccta gaaaaagaac
     2821 gggatcaggt taaaaaccaa ctacttttgc aggaaaagga aaatgaatta tctttggctt
     2881 ctgttaagca aaactacgaa gcccagctca aggcagctag tgaacaagtc gagttttata
     2941 agaattttaa ggctcaacaa tctacaaaag cgattgggga aagcctagaa cagtatgcag
     3001 agagtgagtt taacaaggtt cgtagtttcg cctttccaaa tgcttacttt gagaaggata
     3061 acaaggtctc ttcgcgtggg tctaaagggg actttatctt ccgtgagtgt gatgaaaatg
     3121 gagttgaaat catttctatc atgtttgaga tgaaaaacga agcggacgga acagagaaga
     3181 agcacaagaa tgcagatttt tacaaggaat tggacaagga ccgtcgggag aagaactgtg
     3241 agtatgccgt tttggtgacc atgcttgagg ctgataatga ctactttaac acagggattg
     3301 ttgacgtcag tcacgagtat gaaaaaatgt atgttgttcg tcctcaattc tttatccaat
     3361 tgattggtct cttacgtaat gcggcgctaa attccctaaa atacaagcag gagttggcct
     3421 tggttcgcga gcaaaatatt gacattacgc attttgagga agatttggat gcctttaagc
     3481 tagcttttgc taagaactat aattcagctt cgactaactt tggaaaagct attgatgaaa
     3541 tcgacaaggc catcaaacgc atggaagagg ttaagaaatt cctgaccaca tctgaaaacc
     3601 aactccgttt agctaacaac aaattggaag atgtctctgt taaaaaattg acccggaaaa
     3661 atccaacaat gaaagcgaag ttcgaagcac tgaaggggga gtagaaagca aaaatgaacg
     3721 gtattattaa cttaaaaaag gaagcaggaa tgacctcgca tgatgcggtt tttaaactgc
     3781 gtaagatttt gggaaccaag aaaattggtc atggtggaac cttggatccg gatgtggtgg
     3841 gtgttttgcc gattgcggtt ggcaaggcga cacgcatggt cgagtttatg caggacgagg
     3901 gtaagatcta tgagggggaa atcactctgg gctattccac gaagactgag gatgctagtg
     3961 gggaagtggt cgcagaaacc cctgttttgt ctctcttgga tgaaaagctt gttgatgaag
     4021 cgattgctag cttgactggg cctattactc agattccccc tatgtattcg gcagttaagg
     4081 ttaatggtcg caagctctat gagtatgcgc gtgctggtca ggaagtggag cgtccagaac
     4141 gtcaggtgac catttatcaa tttgagcgaa caagtccgat ttcttatgat ggccaacttg
     4201 cccgattcac ttttcgtgta aaatgcagta aagggacgta catccgtact ttgtcagttg
     4261 atttgggtga aaagcttggt tatgcggctc atatgtccca tttgactcgt actagtgctg
     4321 ctggcttaca attagaagac gctcttgcct tggaggaaat tgctgaaaaa gtagaggctg
     4381 ggcaattaga ttttctccat cctttagaga ttgggacagg tgaccttgtc aaagttttcc
     4441 taagtccaga agaggctaca gaagttcgct ttggtcgttt tattgagcta gaccaaacgg
     4501 acaaagaact ggctgccttt gaagatgata aattgttagc cattctagaa aaacggggca
     4561 atctctataa gccaaggaag gtttttagct agatcgttta ggaataaaaa tcgggtgata
     4621 gataacaatt gcttgataaa accccatact aatagtagaa tggttttggg aattataata
     4681 ttccaattgt tgcgagttgt aggtactcaa ataatctata tagaaattta gaggtgtgaa
     4741 atgaagcaat ttaaaattct ttcagataaa tatttagagt ccattacagg ttctgatggg
     4801 aacttaggcc caggatttgg tgtgataatt ccatgatgcg aaatgagttt cgagaaaggg
     4861 tggagcaact tcttcaacaa aaagaaataa atgaaaatag tgagttgagt cacctgtttc
     4921 gtcttgctat acaaaattta gacagaaatg aaaaatacca atcggtcatg gccaatttga
     4981 gtcaagggtt gtcactttac ctcatgacgc atcattacca ggcacctaag tctgtcattg
     5041 attttggttt atggattgcc aaagctccta gccaggaaag agggagactg gctttcttgc
     5101 agatgcttgc tcaaacccta cagggctttc gttaaaactg tcgaacatca gagggaagac
     5161 tctggtgttt tttgctgatg agactataaa ttttgtaaaa aatgtgatag aatagacgac
     5221 ggataaaata acggaggata gcatgcaaaa tagaccaatc attattggag tgacaggtgg
     5281 ttctggtggt ggtaaaacca gtgtttcaag agccatttta tcgcattttc ctgatgaaaa
     5341 gatttccatg attgagcatg attcatacta caaggatcag tctcatttga cctttgaaga
     5401 gcgtgtcaaa accaactatg accatccttt tgcctttgat acagacttga tgatcgagca
     5461 gattaaggaa ttgttggcag ggcgtccggt ggacatcccg acctacgact atacagagca
     5521 tacacggagt agcaagacct atcgtcagga acctcaagat gtctttatcg ttgagggtat
     5581 tttggtcttg gaggacaagc gtctgcgcga tttgatggat atcaagattt ttgtggatac
     5641 ggatgacgat gtgcgcatta ttcgtcgtat caagcgtgat atggaggagc gtggccgtag
     5701 ccttgatagc gttattaacc agtacttagg tgtggtcaaa ccaatgtacc accagtttat
     5761 cgagtcaact aagcgttatg ctgatatcgt cattcctgaa ggggttagca ataccgtggc
     5821 tatcgacctg ttgacgacca agattgcaaa gattttggaa gaagctcgaa acagcaaata
     5881 atcacatgtg gaggccttgc ctcctttttc tatttttccc tcataatggt acataaatgg
     5941 agatttttag gcatattttt ggtataataa tacccatgaa agagcaagta aatgaatagt
     6001 aggtggagat ggaaaagtat ttatcggtaa caactttgac caagtatctg aaaatgaaat
     6061 tcgataaaga cccatacttg gaacgggtct atttaactgg tcaagtttcc aactttcgta
     6121 aacgacctac tcaccaatat ttctccctaa aggatgacca tgcagttatt caagcgacca
     6181 tctggtctgg gatttatcag aaattagggt ttgacctgga agaaggaatg aagatcaatg
     6241 tgattgggcg tgtacaggtc tatgaaccaa gtggtagcta ctccatcatc attgaaaaag
     6301 ctgagcctga tggggttggg gcgcttgcga ttcagtttga acaacttaag aaaaaattga
     6361 cagaagaagg cctgtttcaa gaacgcttca agcaagctct gccccaattt tctaagagaa
     6421 ttggtgtagt aaccagccgt agtggagccg ttattcgaga tattatcacg accgtcagca
     6481 ggcgattccc aggtgttgac atccttcttt atccgaccaa ggttcaaggt gaaggggctg
     6541 cggaggaaat tgctagaaat attgcgcgtg ctaatcaacg ggacgatttg gatttgctca
     6601 ttattggtcg tggaggtggt tctattgagg atctctgggc ctttaacgaa gaaattgtgg
     6661 tacgagctat ttttgaatct cgtttgccag ttatctctag tgtggggcat gagacggatg
     6721 tgaccttggc agattttgtg gcagatcgac gcgctgcaac gccaacagcg gcggctgaac
     6781 tggcaacacc tgtgaccaag ttggatgtat tagctcattt gcaaaatcag gaaaaacgga
     6841 tggtaacggc agtccgaaat gttctatcta agaaacaaga ggctttgaaa aaatgcagtc
     6901 agtctgttat ctttagacaa cctgagcgct tgtatgacgg ttatttgcaa cgcttggacc
     6961 aactgcaact gcgtttgaaa caaagtttgc gaactcggat ttctgataac aaacaattag
     7021 ttcaagcaag aactcatcaa ttagtacaat tatcacctgt taccaaaatc caacgctatc
     7081 aagaccgttt aggacagttg gacaagctct taggtagcca aatggcgtta gtttatgacg
     7141 ccaaggttgc tgaggccaag cgactttcgg aagctttgct catgttggat actagccgaa
     7201 tcgtggcgcg tggttatgct attgtcaaaa aagaagagtc cgttgtagat tcggttgaga
     7261 gtttgaagaa aaaagaccaa gtaacgcttt tgatgcgaga tggtcaagta gaattagagg
     7321 ttaaagatgt caaaacaaaa gaaatttgag gaaaatctag cagaactgga aaccattgtc
     7381 caaagtttgg aaaatggtga aattgctctg gaagatgcga ttactgcctt tcaaaagggc
     7441 atggtcttgt caaaagagct ccaagctacg ctggacaagg ctgaaaagac cttggtcaag
     7501 gtcatgcaag aagacggaac agaaagtgat tttgaatgaa aaagcaagaa aaattagctc
     7561 ttgtcgagtc ggctttggaa gatttttatg gagaccagca gtttgcctct agtttacggg
     7621 agtctgttct ctattctatt catgctggtg gcaagcgtat tcggcctttt ctcttgttag
     7681 aagttctgga agccttgcag gttaccatca aacctgctca cgcgcaggta gctactgcct
     7741 tagagatgat tcatacaggg agcttgattc acgatgacct tcctgctatg gatgatgacg
     7801 attatcgaag agggcggtta accaatcaca agaaattcgg tgaagctatg gccattttgg
     7861 ctggagatgc cttattctta gactcatatg ccttgattgc gcaggcagat ttgccaagtc
     7921 agattaaggt ggacttgatt gccaacttat cccttgcttc aggtagtctg ggtatggtag
     7981 cagggcaagt tttggatatg gagggcgaac accagcactt gtctttggaa gaacttcaga
     8041 ctattcatgc caataagact gggaagttac tagcctatcc cttccaagcg gcagctatta
     8101 tagctgaatt gtcacctgaa atgcaggtga agctgaaaac tgtgggtgaa ttgattggac
     8161 ttgcttttca agtcagagat gatgtactgg atgtgacagc tagttttgag gaaatcggca
     8221 agacacctca aaaggatctg caggcagaaa aatcaaccta tcctgccctg ttgggcttgg
     8281 aagagtccat tgccttttgt aaccaaaccc tggatcaagc taatgaaaaa ttagaagaaa
     8341 ttgcccagca gcttcccttt gaaacagaat cgattgtaag tgtagtagaa agtttgagaa
     8401 tcaatggcta aggaaagagt ggatgtacta gcttataaac aggggttgtt tgaaacgaga
     8461 gagcaggcca agcgaggtgt gatggctggc ctagtcgtag cagtccttaa tggagaacgg
     8521 tttgacaagc caggagagaa aattccagat gacaccgaat taaaactcaa gggggagaaa
     8581 ctcaagtatg tcagccgtgg tggtttgaaa ctggaaaagg ccttgcaggt ctttgatttg
     8641 tcggtggatg gcgcgactac gattgatatc ggggcctcta ctggaggttt taccgatgtc
     8701 atgctacaga atagtgccaa gttggtcttt gcagtcgatg ttggtaccaa tcagttggct
     8761 tggaaattac gccaagaccc acgagttgtc agcatggagc agttcaattt tcgctatgct
     8821 gaaaagactg atttcgagca ggagccgagc tttgccagta ttgatgtgag tttcatttcc
     8881 cttagtctga ttttgccagc cttgcaccgt gtcttggctg atcaaggtca ggtggtagca
     8941 cttgtcaaac ctcagtttga ggcaggacgt gagcagattg ggaaaaatgg aattattcga
     9001 gatgctaaga ttcatcagaa tgtccttgaa tctgtaacag ctatggcagt agaggcaggt
     9061 ttttcagtcc ttggcttgga cttttctccc atccaaggtg gacatggaaa tattgaattt
     9121 ttagcgtatt tgaaaaaaga aaagtcagca agcaatcaga ttcttgctga gattaaagaa
     9181 gcagtagaga gggcgcatag tcaatttaaa aatgaataaa aaagaaagac ttgaaaaaat
     9241 tagaagattg gtgacagatt atcaaatcgg cacgcaagaa gaaattgtag aacatttgaa
     9301 agaagcaggt atcactgcca ctcaggcgac ggtatcccga gatatcaaag agttaggtat
     9361 tgtcaaaatt cctttgagag acaacaccta tgtctacgaa ttaccaaaat caatcgtaaa
     9421 aagtctgcaa ctggctgaag acaatatcga atcggctgaa ttgatggata agatgatcaa
     9481 tctccaagtt attccaggaa atacggcttt tgtaaaagct cagttaatcg atacttttgc
     9541 agacaagatt tttagttgtt tgactgatga tagctcgatt ttagtcattg ccagaagtgg
     9601 aagtctagca gaggaaatct ttgaacaagt aaaaaattgg taggtctata tgttacttga
     9661 aatttcgata aaaaactttg ccattattga ggctatttcc ctcaattttg aaaaggggat
     9721 gactgtcctg actggtgaaa cgggtgcagg gaagtcgatt atcattgatg ccatgaatat
     9781 gatgttggga gctcgtgcga cgacagatgt tattcgtcat ggtgcaccaa aggcagagat
     9841 tgaggggctt ttctcagttg agaatagtcg ccttttacag gaaatttttg atgagcaagg
     9901 tttagaatta ggtgatgaaa ttatcatccg tcgggaaatc ttgcaaaatg gtcgtagtat
     9961 cagccgtgta aatggtcaga tggttaatct gtctgttttg cgagctattg ggcaacatct
    10021 tgtagatatt catggtcagc atgaccatga ggagttaatg cgtccccaac tgcatatcca
    10081 gatgttggat gaatttggtg atgccgcttt ttgggacttg aaagaaacct atcaaacgag
    10141 ttttgatgcc tatcggaaaa tgcgcaagca ggttctggaa gtcaagaaaa accaacagga
    10201 acacaaggca cgtatcgaaa tgttggaatt tcaaatggca gagattgagg cagcaaactt
    10261 gcaggctgga gaagacttgg ctctcaatca agagcgagat aaactcctca accacaaaaa
    10321 tattgcggat acactgacca atgcctacag tatgttggac aatgaagatt tttcaagtct
    10381 ggccaatgtt cgttcagcta tgaatgacat ggaaagtgtc gaagagtatg atcctgaata
    10441 ccgtgaaatt tcaagctctc tgtctgagac ctactatgtt ttagaagaca ttagcaaacg
    10501 tttggaagct atcattgagg accttgattt tgatggcaat cgcctgatgc aggttgagaa
    10561 tcgtttggac ctccttcata ctattactcg taagtatggt gggactgttg atgatgtttt
    10621 gctttatttt gccaagatta cggaagaata caatctcttg acaggcaata atctttcgtc
    10681 tgaggacatg gaagcagagc ttaagaagtt ggaagtcaat cttgtcaatt tggcaggtca
    10741 acttgcttct gctcgtcata atttggctca gcaactcgaa gctgagatta aacaagaact
    10801 gcaagatctt tatatggaaa aagcccagtt tcaggttcgt tttagtaagg gaaaattcag
    10861 tcgtgaggga aatgaaatgg ttgagtttta tatttcaacc aaccctggag aagactttaa
    10921 acccttggtt aaggttgctt ctggagggga attatctcgt ctcatgttag ccattaagtc
    10981 tgccttttca cgtaaagaag gcaagactag cattgtcttt gatgaggtgg atacgggagt
    11041 ttcaggtcgt gttgctcaag ctattgctca gaaaattcat aaaattggtc agcatggtca
    11101 ggttttggct atctcccatt tgccacaagt aattgcgatt gcagattatc aattctttat
    11161 tgagaagatt agtaatgacc attcaacggt ttcgactgtt cgtctcttga cggtcgaaga
    11221 gcgagtggag gaagttgcca agatgttggc aggtgatgat gtgacagaag cagccctgac
    11281 gcaagccaga gaattgttga gaaacaggga gaaataagat gacagattat tatgtaattg
    11341 gagatgttca cggaaaagct gggatgctgg aagaccttct caaaacctgg gatggtcagc
    11401 cccagttgct ctttctaggt gatttgattg acagaggtga ggatagtcac cgtgttcttg
    11461 aaatggtcaa ggacttggtg gacaatcaag gggctatctg tttgtccgga aaccacgagt
    11521 atatgttttt gacttggcta gatgacccag aagaaagtta tgaccattat cgtcgcaatg
    11581 gtggagatac aaccattaac tctatcctag gtcgtccctt ggatgcacca gttgatggag
    11641 tagaagatgc caagcgggtt gccgctgaag cgtcagactt ggtcgaattc attcgtcaaa
    11701 tgccatttgt agtagagaca gacaagtata tatttgttca cgcaggtatt gatttgactt
    11761 tggacgactg gcatgaaaca acagattata aaaaagtatg gcttagaaaa ccattccacg
    11821 gagctgaaaa tcatactgga aaaaccattg tcttcggtca tacaccggtc tatggtttgt
    11881 tacagcaaga acgcggtaca tctgagcttt ggactacaga agatggcaag attggaatgg
    11941 atggaggagc tgtttatggt ggtgtccttc acgggattgt ctttacagac caaggaatga
    12001 cagaacacca ctttatcgaa aatgacggct ttgtcgctga agattagtac tcctagcagg
    12061 gtatggtctt gtcaaaatat caaaaacaat ttataataaa tagataccct gaaaggaaga
    12121 gaatcatgaa cttagaagaa ttgaaaaaac gacagaggaa gatccgtaac ttctctatta
    12181 tcgcccatat tgaccacggg aaatcaactc tagcagaccg cattttggaa aaaacagaga
    12241 cggtttcaag tcgtgaaatg caggcccagc ttttggatag catggatcta gagcgggaac
    12301 gtggaattac tattaagttg aatgccatcg agttgaatta cactgcaaaa gatggggaaa
    12361 cttatatttt ccacttgatt gacacaccag ggcacgttga ctttacctat gaagtttcac
    12421 gttcgctagc tgcctgtgag ggtgctattt tggtggtcga tgcggctcaa ggaattgagg
    12481 ctcaaactct tgccaacgtt tatctggctt tggataatga tttggaaatc atgcctatca
    12541 ttaataaaat tgacctgccg gctgcagatc cggagcgcgt gcgtacagag attgaagatg
    12601 tgattggttt ggatgccagt gaagcagttt tggcttctgc caaggctggt attgggattg
    12661 aagaaatcct cgagcaaatt gtagaaaaag taccagcacc aacgggtgat gtgacggcgc
    12721 cacttaaggc cttgattttc gactctgttt acgatgctta ccgtggggtt atcctccaag
    12781 tgcgtgtcat ggacggagtg gtcaaacctg gtgataagat tcagctcatg agcaatagta
    12841 agacctttga tgtggccgaa gtcggtattt ttacaccaaa agcggttggt cgtgatttcc
    12901 ttgcgactgg tgatgttggt tacattgcgg cgtctatcaa gacggttcag gatactcgtg
    12961 tgggtgatac cgttaccttg gcaaccaatc ctgcggcaga accattacat ggttataagc
    13021 agatgaatcc tatggtcttt gcgggtctct acccaatcga atcaaacaaa tacaatgacc
    13081 tgcgtgaagc gcttgaaaaa ttgcaactga atgatgctag tcttcagttt gaaccagaaa
    13141 catctcaggc acttggattt ggtttccgtt gtggatttct tggacttctc catatggatg
    13201 ttatccagga acgtttagag cgtgagttca atattgacct catcatgaca gctccgtctg
    13261 ttatttacaa agttaatttg accgacggtg agtctatgga tgtgtctaac ccatctgagt
    13321 ttccagaccc aactaagatt gcgaccattg aagagcctta tgtcaaggcg caaatcatgg
    13381 taccacagga gttcgtcgga gcagtaatgg agctagctca gcgtaagcgt ggggactttg
    13441 tgactatgga ctatattgat gataaccgtg tcaatgttat ctatcaaatt cctcttgctg
    13501 aaattgtctt tgacttcttt gataaactta agtcttcgac acgtggttat gcaagctttg
    13561 actacgaatt gtcagaatat cgcccatcta agctggtgaa aatggatatc cttctcaatg
    13621 gagataaggt ggatgccctc agctttatcg ttcacaagga ctttgcctac gaacgtggga
    13681 aactcatcgt tgataaactc aagaaaatca tccctcgcca acaatttgaa gttccaatcc
    13741 aagcggctat tggacacaag attgtcgctc gtactgatat caaggccctt cgtaagaacg
    13801 tacttgctaa atgttatggt ggtgacgttt ctcgtaagcg taaactcctt gaaaaacaaa
    13861 aagctggtaa gaaacgcatg aaatccatcg gatcagttga agttccgcaa gaagccttcc
    13921 tcagcgtctt gagtatggat gaagaataag ttatcagaaa tgcctttcct tgttcacagg
    13981 aaatttatat aagaatcaat agtaaagcag gcttagatgg tctgcttttt tagtttttat
    14041 actcttcgaa aatctcttca aaccacgtca gcgtcgcctt accgtatgta tggttactga
    14101 cttcgtcagt ttcatctaca acctcagaac catgttttga gctgacttcg tcagtttcat
    14161 ctacaacctc aaaatcatgt tttgagctga cttcgtcagt cttatctaca acctcaaaac
    14221 tgtgttttga gtaacctgcg gctagcttcc t
//
LOCUS       DAVHEX010000056        12699 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVHEX010000056 DAVHEX010000000
VERSION     DAVHEX010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12699
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.056"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            473..1594
                     /gene="mnmA"
                     /locus_tag="VVL45_001700"
     CDS             473..1594
                     /gene="mnmA"
                     /locus_tag="VVL45_001700"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEU4187847.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAITLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRRLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGEKTEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            1735..2190
                     /locus_tag="VVL45_001701"
     CDS             1735..2190
                     /locus_tag="VVL45_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU4187848.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALIVQNHKLLVTKDKGKYYTIGGA
                     IQVNESTEDAVVREVKEELGVKAQAGQLAFVVENRFEVDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLQNIQLVPVFLKTALPDWEGQLRHIHLEE"
     gene            2200..4113
                     /gene="mnmG"
                     /locus_tag="VVL45_001702"
     CDS             2200..4113
                     /gene="mnmG"
                     /locus_tag="VVL45_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEU4187849.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEVPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            complement(4320..4412)
                     /locus_tag="VVL45_001703"
                     /pseudo
     CDS             complement(4320..4412)
                     /locus_tag="VVL45_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(4466..6145)
                     /locus_tag="VVL45_001704"
     CDS             complement(4466..6145)
                     /locus_tag="VVL45_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU4187850.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(6147..6380)
                     /locus_tag="VVL45_001705"
     CDS             complement(6147..6380)
                     /locus_tag="VVL45_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002947111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEU4187851.1"
                     /translation="MIYKVFYQETKERSPRRETTRTLYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            6660..6875
                     /locus_tag="VVL45_001706"
     CDS             6660..6875
                     /locus_tag="VVL45_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187852.1"
                     /translation="MYNLFAFAIGARLVAFIVFVACVYAGNLLFAKLERRQTKFLWKM
                     TFVTLYSLFLLLVTYVVWFVFYFELNA"
     gene            complement(7198..7350)
                     /gene="cibB"
                     /locus_tag="VVL45_001707"
     CDS             complement(7198..7350)
                     /gene="cibB"
                     /locus_tag="VVL45_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HEU4187853.1"
                     /translation="MMKDLNNYREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(7352..7537)
                     /gene="cibA"
                     /locus_tag="VVL45_001708"
     CDS             complement(7352..7537)
                     /gene="cibA"
                     /locus_tag="VVL45_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HEU4187854.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            7715..8398
                     /gene="tsaB"
                     /locus_tag="VVL45_001709"
     CDS             7715..8398
                     /gene="tsaB"
                     /locus_tag="VVL45_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEU4187855.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMASLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAHTLNIELVGMSSLLALVPH
                     QQEGLFVPLMDARRNNVYAGFYENAKPVMAEAHLSFEEVLEKVKGTSQVTFVGEVGPF
                     VEQIQKHLPRTDYKETLPNAANLALLAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            8395..8832
                     /gene="rimI"
                     /locus_tag="VVL45_001710"
     CDS             8395..8832
                     /gene="rimI"
                     /locus_tag="VVL45_001710"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEU4187856.1"
                     /translation="MIEIKRIQQQPDLAQAIYAVMAAVYLVSPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLAVQENLFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RQSNQRAQAFYKKEKMTVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            8822..9832
                     /gene="tsaD"
                     /locus_tag="VVL45_001711"
     CDS             8822..9832
                     /gene="tsaD"
                     /locus_tag="VVL45_001711"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEU4187857.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLHHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKILVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(9878..11178)
                     /locus_tag="VVL45_001712"
                     /pseudo
     CDS             complement(9878..11178)
                     /locus_tag="VVL45_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(11454..11805)
                     /locus_tag="VVL45_001713"
                     /pseudo
     CDS             complement(11454..11805)
                     /locus_tag="VVL45_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(11912..12205)
                     /locus_tag="VVL45_001714"
                     /pseudo
     CDS             complement(11912..12205)
                     /locus_tag="VVL45_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            12448..>12699
                     /locus_tag="VVL45_001715"
     CDS             12448..>12699
                     /locus_tag="VVL45_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEU4187858.1"
                     /translation="MQYSCGKININIPDGYGDIKDIVFSAHIIVRYNNGHCGGIDPHI
                     IGLCKKQIRRMSLYPILIIVSRDSKVIDDYKNLDIAYVDC"
BASE COUNT         3602 a         2512 c         2951 g         3634 t
ORIGIN      
        1 ggcttaggtg cccttgcagt caacacaact gtagatggct ataaagtcaa tgccaatggt
       61 gaatgggttt aagccgatta aattaaatca tgttaagaac atttgacatt ttaattttga
      121 aacaaagata aggttcgatt gaatagattt atgttcgtat tctttaggta cctatcttat
      181 gatttcagga aatgtcatta aaaaaacgac tcattttctc taacttgaaa aatagattag
      241 agaaaatggg ttgttttatt tattatagtt atttgaatga agataagaag aaggtatact
      301 cacatcattc acataatctg tatattgact ataagtttta aaaaacaatt tttaagctct
      361 tccttgtctt ctctaaccaa gcgtgttata atgaatactg ctcaagcgac cttcaatcgt
      421 gaagcacaca cgaccttcaa tcgtgaataa acgaatagat gggagactta ccatgagtga
      481 taactctaaa acacgtgttg tcgtggggat gagtggtggt gttgattcgt cggtgacggc
      541 tcttttgctc aaggagcagg gctacgatgt gatcggtatc ttcatgaaga actgggatga
      601 cacagatgaa aacggcgtct gtacggcgac cgaagattac aaggatgtgg ttgcggtggc
      661 agaccagatt ggcattccct actactctgt caattttgaa aaagagtact gggaccgcgt
      721 ttttgagtat ttcctagcgg aataccgtgc agggcgcacg ccaaatccgg acgttatgtg
      781 caacaaggaa atcaagttca aggccttttt ggactatgcc ataaccttgg gggcagacta
      841 tgtagcgact gggcattatg ctcgagtggc gcgtgatgag gatggtaccg ttcacatgct
      901 tcgtggcgtg gacaatggca aggatcagac ctatttcctc agccaacttt cgcaagaaca
      961 acttcaaaaa accatgttcc cactaggaca tttggaaaag cctgaagtac gcagactagc
     1021 agaagaagca ggcctttcga ctgctaagaa gaaagactcg acagggattt gctttatcgg
     1081 agaaaagaac tttaaaaact ttctcagcaa ctacctgcca gctcagcctg gtcgcatgat
     1141 gactgtggat ggtcgcgata tgggcgagca tgcaggtctt atgtactata caatcggtca
     1201 gcgtggcgga ctcggtatcg gtgggcaaca cggcggtgac aatgcccctt ggttcgttgt
     1261 cggaaaagat ctaagcaaga atattctcta tgtaggacaa ggattctacc atgattcgct
     1321 catgtcaact agcctagaag ccagtcaagt ccactttact cgtgaaatgc cagaagagtt
     1381 tacgctagaa tgtacggcta aattccgtta ccgtcagcct gactctaagg tgaccgttca
     1441 tgtcaaagga gaaaagacag aggtcatctt tgcggaacca caacgcgcga ttacaccagg
     1501 acaggcagtt gtcttttacg atggcgaaga gtgtctaggt ggcggtttga ttgacaatgc
     1561 ttaccgcgat ggacaagttt gtcagtacat ttagattgac aaattttctc aatttgctac
     1621 aataataaca gcaatagaaa tgatggtcaa agctcatgga tgttgcaggc ttttttgtcc
     1681 tgcacttctt tgtagttttg actgtttttg tgtcgtttaa gggaaaggac aagaatgact
     1741 caacaagact ttcggacaaa agtagacaat acggtttttg gtgttcgggc tacagccttg
     1801 attgtccaaa atcacaagct tctagttacc aaagacaagg gcaagtatta cactattgga
     1861 ggtgcgattc aagtcaatga aagcacggaa gacgcggtag tccgtgaagt gaaggaagaa
     1921 ctgggtgtca aagctcaagc tgggcagcta gcttttgtgg ttgaaaatcg ttttgaagtg
     1981 gacggcgttt cctatcacaa tatcgagttt cattatctgg tggatttgct tgaagatgcc
     2041 ccgttgacca tgcaggaaga tgagaaaagg cagccctgtg agtggattga cttggataag
     2101 ctccagaata tccagctagt tccagtcttt ttaaaaacag ccctaccaga ttgggaaggc
     2161 caactaagac acattcatct tgaggaatag gagagaaaca tgacttatca ttttactgaa
     2221 gaatacgata ttattgtaat tggtgcggga cacgctgggg ttgaggcttc cttggccgct
     2281 agccgtatgg gctgtaaggt cctgcttgcg accatcaata ttgaaatgct ggctttcatg
     2341 ccttgtaatc cctctatcgg tggttctgcc aaggggattg tcgtgcgtga agtcgatgcc
     2401 ctcggtggcg agatggccaa aaccattgac aagacttaca tccagatgaa gatgctaaac
     2461 acagggaagg ggccagctgt ccgtgccctt cgtgcgcagg ctgacaagga actttactct
     2521 aaggagatgc gcaagacggt tgaaaaccaa gaaaatctga cccttcgtca aaccatgatt
     2581 gatgagattt tggtggaaga tggcaaggtt gtcggtgtgc gtacagccac ccatcaagaa
     2641 tatgctgcta aggctgttat tgtgacgaca gggactgctc tccgtgggga aattatcatc
     2701 ggagacctca agtactcatc aggtcctaac cacagcttgg cttctattaa cctagctgac
     2761 aatctcaagg aactgggtct cgaaatcggt cgtttcaaga caggaacccc tccacgtgtc
     2821 aaggcttctt ctatcaatta cgatgtgaca gaaattcagc caggagacga agtgcctaat
     2881 catttctcat acacttcacg tgatgaggat tatgtcaagg accaagtacc atgctggttg
     2941 acctatacca atggtaccag tcatgagatt atccaaaaca acctccaccg tgcgcctatg
     3001 tttacaggtg tggtcaaggg agtggggcct cgttactgtc cgtcgattga agacaagatt
     3061 gtgcgctttg cggacaagga acgtcaccaa ctcttccttg agccagaagg gcgcaatact
     3121 gaggaagtct atgtgcaagg actttcaacc agtctgcctg aggatgtcca gcgtgacttg
     3181 gtgcattcca tcaaaggttt ggaaaatgca gagatgatgc ggacaggtta tgctattgag
     3241 tatgatatgg tcttgcctca tcagttgcgt gcgactttgg aaaccaagaa aatctcaggt
     3301 ctcttcactg ctggtcagac aaatggaaca tcaggttacg aagaggcagc aggccaaggg
     3361 attatcgcgg gtatcaatgc ggctctgaaa atccaaggca agcctgaatt gattttgaag
     3421 cgcagtgatg gttatatcgg ggtgatgatc gacgacttgg tgaccaaggg aaccattgaa
     3481 ccctaccgtc tcttgaccag tcgtgctgaa taccgtctca ttcttcgtca tgacaatgct
     3541 gatatgcgct tgactgagat gggacgcgag attggccttg tggacgatga acgctgggct
     3601 cgttttgaaa tcaagaaaaa tcaatttgat aatgagatga agcgcctaga cagtatcaaa
     3661 ctcaagccag tcaaggaaac caatgccaag gttgaggaga tgggcttcaa acccttgacc
     3721 gatgcagtga cagccaagga attccttcgc cgtccagaag tttcttacca agatgtggtg
     3781 gccttcatcg gaccagctgc agaagacttg gatgacaaga ttatcgaatt gattgaaaca
     3841 gaaatcaagt atgaaggcta tatttccaaa gccatggacc aggttgccaa gatgaaacgc
     3901 atggaagaaa aacgcattcc ggccaatatc gactgggatg acattgattc tatcgcaacc
     3961 gaagcccgtc agaagttcaa actcatcaat ccagaaacca tcggccaagc cagccgtatt
     4021 tcgggagtaa acccagcaga tatttctatt ttgatggtgt atctggaagg taaaaatcgt
     4081 agtatttcta aaactcttca aaaatcaaaa tgatacgtcg tcggcttctt acgaatgagt
     4141 tcaaagcttg gctttgattc atctccagcc tcccatagtt tcccgaacta tgggagctaa
     4201 ctcaagttat gctttcgctt agccttctag tctgatttcg atttttattg atactcttcg
     4261 aaaatctctt caaaccgcgt caacgtcgcc ttgccgtagg tatggctact gacttcgtca
     4321 gttctatcta caacctcaaa gcagtgcttt gagcaaccta cggctagctt cctagtttgc
     4381 tctttgattt tcattgagta tgagttttaa tctcaaaaga aagcatagag aaaaacagct
     4441 ccgaggacgg ggctgttttc ttgctttatt cttcatctgg tgtgaggatc atcgggatga
     4501 tgatcggttc acgttcggta ttttcataga ggaaggggcg aatagcgttg acaatggcac
     4561 cattgacaga ttgcacgcta gcatccttat ttttcagtgc gatacgaatg gcattgaaga
     4621 ggatacgctg gctttggcga atcaagtcgc cagactctct catgtagaca aagcctcggc
     4681 tgaggatgtc tggaccagat agaatcatct gcgatttgaa gtcaacagtt gcaactgcca
     4741 gaacgacacc gtcttcagat agatcgcgac gatctttgag gacagctgcg ccaatttcac
     4801 cgatacgatt tccatcgaca tagatatctt gggcgttgaa atgacctgcg atacgagctg
     4861 agtcagcagt aagggcaagc acatcgccat tgctcatgat aaagatattg tccttctcaa
     4921 caccagtatc cactgctagt ccagcgtgga ctttttgcat gcggtattca ccgtggacag
     4981 gcatgaagta ttttggctta atcaagcaga gcatgagttt ttgctcttgc tgaccaccgt
     5041 gtccagatgt atggatattg ttcactttac cgtggataac ttcgacacca gcttcagaaa
     5101 tgatgttaat cagcttgttg acactagtag tgtttccagg gatgggacta gaagagaaga
     5161 taacggtatc acctggttgt aattgtactt gacggtgggt tccgttggcg atacgagaga
     5221 gggctgccat aggctcaccc tgactacctg tacagaggat aagaacttct cctgcaggat
     5281 aatctttgat ttcatttggc tcgataaagg ttcccttagg agctttgatg tagccaagat
     5341 cgattccgtt gacaatggcc ttttccatag aacgaccaaa gaccgcaatc ttgcgtccag
     5401 tcttaacagc agcttctgtt gcctgctgga gacggaagat atttgaggca aaggatgcaa
     5461 agatgatacg tccttcaata ccttggataa tcttcataat ggactgacca acgacttttt
     5521 cagagttggt aaaggttggt acttccgcat ttgtcgagtc agacaggaga cagagcacgc
     5581 cttcttcacc aagcgcagcc atacgatgca agtccgcagg ttctccaact ggagtaaagt
     5641 cgaacttaaa gtcacccgta cagacgattt tcccttgagg agtatgaatg acaatcccca
     5701 aaggctctgg aatagagtga gtcgttctaa agaaagttgc cttgagattt ttaaaggtca
     5761 actcggtgtt gtggttgatt tcgtaaagtt tggcgttgcg caagaggccg tgttcttcga
     5821 gtttcccacg gatcaaagcc aaggcaagcg gtccagcata aatagggaca tttgcttgct
     5881 tgagtaggaa cggaatccca ccaatgtggt cctcgtgtcc gtgtgtgatt aaaacagcct
     5941 tgacgcggtc gatattgtcc acgatgtaag agtagtcagg aatgacatag tcgataccaa
     6001 gcaagtcatc ttctgggaat ttaatcccag catcgacgat gataatctcg tcttggtatt
     6061 caattccgta agtgtttttc ccgatttctc ctagaccacc gatggcaaaa acgccgactt
     6121 cttcaggttt aagagtgtag gccatattag aactccgtaa tttcgaaggc gccagtttct
     6181 ttttcgtaat cgagcaattt gtcagacaag agttcgatat actcgatatt gtactctggg
     6241 cgattttctt cgacaagttg gcgagcagtg atacggccct caagttctga gctggcatcg
     6301 atgtctaggt aaagcgtgcg tgttgtttca cggcgtgggc tacgttcttt tgtttcttga
     6361 taaaaaactt tgtaaatcat atagttcctt tctatttaca ggtcagctta ttccaaactt
     6421 ttagcagata tttgcttgat ttcattccat tttgtgtcta gattgacctt gattcgttac
     6481 aattatttca attataccac aaaatgaaaa attagtaaaa gacggaaacg agaaagttag
     6541 gggacgattt ctttggtaag cgttttcaaa aataaagaaa atgtattatg ttgataacgg
     6601 aagtgaaatt tattgcttgt cataattcca tatagatttt aagtcaagga ggttgcccta
     6661 tgtataactt atttgctttt gctatcggag ctcgcttggt agcttttatt gtttttgttg
     6721 cttgcgttta tgcggggaat cttctatttg ccaaactgga acgacgacaa accaagttct
     6781 tgtggaaaat gacctttgtg actctctact cgctttttct cctcctcgtg acctatgtcg
     6841 tttggtttgt attttacttt gaattaaatg cttagatgcc ctgcttatgc tggggttttt
     6901 tgtttgggaa tgtagagata gtagatttac gacacgaaaa ttccacccca tctatcaaga
     6961 tgaggtggtc tttgcatttt tctaacatga catgacaatg gaattttcaa agcttattat
     7021 ttttatggga gtcattgttc tgatcaaaaa tagagttgaa gcataaaaat aaaacaatac
     7081 aaacagataa atcgaatggt aaagggatgg tagtaatgtt caaatcattg agaatgctga
     7141 gcaaactgta agagatgaag acaataaaat ttatccttct aaaaatgtgt ttcttatcta
     7201 gcatgactta ccaaactttt tacgaaggtc ttttccaatg gtataacctg ccataaataa
     7261 agcgatacca acaccgacac caaagccacc cttgatttct tgcaattcct tattagaaat
     7321 ttcacgataa ttgttcaaat ctttcatcat attaatctcc tcctaaaaaa taaccagctg
     7381 ccaaaccaga acctataagt gctgttccac ctgcaatagc cttccaagat actgctactc
     7441 caaagataac caagggagcg agaccgccat caatatctga taattcattt tcagataagg
     7501 ataaaaattg attgtcaaga atgtcaaaat ttgtcattga actttcctcc ttttttcttg
     7561 ttcactttta tattcggaaa agaaaaggaa aagtgagcat ttttttaaat tatttttagt
     7621 taagtgaggg cactcttgtc tgggggcaaa gaaaattccc atgtgccagc ttttgtagta
     7681 taatagtaag cagacaaaaa gataggaatg tgttatgaaa gtattagctt ttgatacgtc
     7741 cagcaaggct ctttctctgg ctattttaga ggataagcag gttcttgccg agacgacgat
     7801 taatattaag aaaaatcaca gtattactct tatgcctgcc atcgattttt tgatggcaag
     7861 tttggattgg acacccaagg atttggaccg aatcgtggta gctgaagggc cgggtagcta
     7921 tacaggcttg cgaattgcgg tagcaactgc taagacctta gctcacaccc tgaacatcga
     7981 gttggttggt atgtcgagtc tcttggctct ggtgccccat caacaagaag gtttgtttgt
     8041 ccccttgatg gatgcgcgtc gcaataatgt ttatgcagga ttttatgaaa atgccaaacc
     8101 tgtcatggca gaagcgcacc tatcttttga agaggtgcta gaaaaagtca agggtactag
     8161 tcaggtaacc tttgtcggag aagttggccc ctttgttgag cagattcaaa aacacttgcc
     8221 aaggactgat tacaaagaaa cattgcccaa tgcagctaat ctagctcttt tggcctggga
     8281 caaggaagca gactccttgc atgattttgt gccgaattac ctcaaacgag tcgaggctga
     8341 ggaaaactgg ctcaagaacc ataccgagtc tggcgagtct tacattaaac gcctatgata
     8401 gaaatcaagc gaattcaaca acagcctgac ctagctcaag ccatctacgc tgttatggca
     8461 gctgtttacc tagtcagtcc ttggactctg gagcaaatcc aagcagatct gtcccaagac
     8521 cagacttggt atgcattggc ttatgatggg gcagaagtga ttggatttct agctgtgcag
     8581 gagaatcttt ttgaagcaga agtcctgcaa atcgctgtca aaggagctta tcagggtcag
     8641 gggattgcgt cagccttgtt tgctcaattg ccgacagaca aggaaatttt cctcgaagtc
     8701 agacagtcaa atcaacgagc gcaagcattt tacaagaaag aaaagatgac agttatcgct
     8761 gagcgaaagg cctactacca tgacccagtc gaggacgcca ttatcatgaa gagagaaata
     8821 gatgaaggat agatatattt tagcatttga gacatcctgt gatgagacca gtgtcgccgt
     8881 cttgaaaaac gacgatgagc tcttgtccaa tgtcattgct agtcaaattg agagtcacaa
     8941 acgttttggt ggcgtagtgc ccgaagtagc cagtcgtcac catgtcgagg tcattacagc
     9001 ctgtatcgag gaggcattgg cagaagcagg gattaccgaa gaggacgtga cagctgttgc
     9061 ggttacctac ggaccaggct tggtcggagc cttgctagtt ggtttgtcag ctgccaaggc
     9121 ctttgcttgg gctcacggac ttccactgat tcctgttaat cacatggctg ggcacctcat
     9181 ggcagctcag agtgtggagc ctttggagtt tcccttgcta gccctcttgg tcagcggcgg
     9241 acacacagag ttggtttatg tttcggaggc aggagattat aagattgttg gggaaacccg
     9301 tgatgatgcg gttggtgagg cttatgataa ggtcggccgt gtcatgggct tgacctatcc
     9361 tgcaggtcgt gagattgacg agctggctca tcaggggcag gatatttatg atttcccccg
     9421 tgccatgatt aaggaagata atctggagtt ctccttctca ggtttgaaat ctgcctttat
     9481 caatcttcat cacaatgccg agcaaaaggg agaaagcctg tctacagaag atttgtgtgc
     9541 ttccttccaa gcagcagtta tggacattct catggcaaaa accaagaagg ctttggagaa
     9601 atatcctgtt aaaatcctag ttgtggcagg tggtgtggca gccaataaag gtctcagaga
     9661 acgcctagca gccgaaatca cagatgtcaa ggttatcatc ccccctctgc gactctgcgg
     9721 agacaatgca ggtatgattg cctatgccag cgtcagcgag tggaacaaag aaaacttcgc
     9781 aggctgggac ctcaatgcca aaccaagtct tgcctttgat accatggaat aaagagtggc
     9841 tctttgtcaa gtgtagtggg taggcgaaaa gctgcaatct ggagagtacg aaattcgtac
     9901 tctctttttt gatgttcaaa gtcatcaaaa tccgtttttt aaaattgtta aagttccgga
     9961 aaccaaaggc tttgcgcttg ataatcttaa tcaaattatt ggtcgcttca agtttagcgt
    10021 tggaataagg cagcttaagt gcgttgataa tcttatctct atcccttaaa aaagtccaaa
    10081 agaccgtttg aaaaagcgga tgaaccgttg gcagttcttg ctcaatcaat ccaaagaaat
    10141 ggtcggcatt cttctcttga aaatgaaaga gcaggagttg atagagttcg tagtgaacct
    10201 gtaatccctc agagtaagaa agcagcttat ctcgtacttc ccgatgggtt aagtgcattc
    10261 gaaacgtggg ctgatagaat cgtttctgac agagcttgaa gctatactgg tgtagtaaat
    10321 cagtccagtg gactgattta gcccgagcct agaaacgaaa aagcgagggt tccaaaagcg
    10381 cttgagcgcc cgatactcca aggattttcg gtcaaattgg ttcatgattt ggattcgtac
    10441 tcggttcata gctcggctca gatgttggac aatgtggaag cggtcaagaa caatcttcgc
    10501 ctttggaaag agttgcttag cgagccgata ataagggctg tacatgtcca tggtgatgac
    10561 ctcgaccagc tcccgaacct ctctctgata gcgttggaaa tggtttcgaa tcaccgcatg
    10621 agttcgcccg tctaaaattg cgaggatgga tttggactca aaatcttggg caatgaagct
    10681 cattttgctc tttttgaagc tatactcatc ccaactcata acttttggca acttggtcca
    10741 atcggtttca aacttaaatt ccctcagttt tcggatgacg gtggaagttg agaccgccaa
    10801 tctgtgggca atctcagtca tagactgctt ttcaaggagg agctgagcga ttttttgtct
    10861 caccatgttg gaaatctggc aatttttctt gacaatggat gtttgagaaa cgaccacctt
    10921 aaggcaattc ttgcactgaa agcggcgctt tttgagatgc agtaccgtgg gtaaaccctg
    10981 acagtcgaga agcggaattt tagaggcttt ctggaagtcg tatttgatca tcttcccttg
    11041 acaatgagga caaggaggag cgggggaatc caactttgcc tgaacgacta gatgggtttg
    11101 gtatttcaga acagacaaga ttttgatatt tttgtcttcc aatccgagca aatctgtggt
    11161 attcttaagt tgttccatat gaaccctttc taatgagttg gttagacgct tttcattata
    11221 ggtcatatgg ggcttttttt ctacaagaaa cgaccctata attcctgggg tgggattacc
    11281 cactacagaa attatagagc caaaacattc caaagtcttg tctgactctg gaatgttttc
    11341 tgttcacagt tgttttgtaa ctgcctaata agtaaccgcg ctataacgag gtatctaccg
    11401 agattgtcaa ttctccagta tactgttata aaaaacaaaa ctggaggatt tttttatgtc
    11461 cccccatcgt cccatcgact gttccgtagc tgcccaataa cggagtatag tcggctgaaa
    11521 caagaatagg acaaaagtgc ctcaagaaaa gcatcgtaat ttgacaatat tattctgaga
    11581 tgtttttgat gtaagcccat attttctcaa tgggattata ctcgggtgaa taggtaggaa
    11641 gtggtaacag tctatgccct gctccttaca tatgttcatt ctgtgaaacc ttgcattgtc
    11701 cataatgata agggatggtg tatctaaagt aggtagtaag aatttttgga agcaagcttc
    11761 gaaaaagtca ctcgtcatcg tatctttata ggtcatcgga gccatagtat attgaaacaa
    11821 aaaagggcat tagtacttct ataacagtat cgcaatctct ggtgtgcgtt gtctcgatct
    11881 taattcaaat aattatatct tcttccagag agactaactg cctgctcaag gctcgatcat
    11941 attcgagata aaaataggtc tcaacccctg tctcataaat ataaatagga gtcaggtagc
    12001 ttaagtgatt caattcttta aggaaccgat ttactttttc tgggtcttgt tcgcagtagg
    12061 tacggctctt ttttagacta tatcccatag cctttagagc gtaatgaata gttgttggag
    12121 gacagttgaa ttcagcagct atttcagtca aataagcatc gggatgagtt tcaatataga
    12181 tttttaattt atctctatca attttttcaa ttaatagtgg aagttttctt gaagttagtg
    12241 tattgaaacg tttttataca gtgctaaaat ttgaagtgta gtgcagatgg atttgtaata
    12301 gtattatagc aattctttgg agaaggtcga gacaagataa aataggtatc tttgagatat
    12361 aagcatgatt tactataaaa ttattgagtt attcataaaa atttctctat caaatatcat
    12421 aatgcgtcgt ttaaggagat ggttacaatg caatatagtt gtggaaaaat aaatattaat
    12481 atccctgatg gatatggaga cattaaggat atagtgtttt cggctcatat tatagttagg
    12541 tataataatg gacattgtgg aggtatagat ccgcatataa ttggactttg taaaaagcag
    12601 atcagaagga tgtctctata tcctattttg ataattgtat ctagagattc aaaggttatt
    12661 gatgattata aaaatttaga tattgcctat gttgattgt
//
LOCUS       DAVHEX010000057        12293 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVHEX010000057 DAVHEX010000000
VERSION     DAVHEX010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12293
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.057"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            206..2866
                     /gene="rrgA"
                     /locus_tag="VVL45_001716"
     CDS             206..2866
                     /gene="rrgA"
                     /locus_tag="VVL45_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus tip adhesin RrgA"
                     /protein_id="HEU4187859.1"
                     /translation="MKKVRKIFQKAVAGLCCISQLTAFSSIVALAETPETSPAIGKVV
                     IKETGEGGALLGDAVFELKNNTDGTTVSQRTEAQTGEAIFSNIKPGTYTLTEAQPPVG
                     YKPSTKQWTVEVEKNGRTTVQGEQVENREEALSDQYPQTGTYPDVQTPYQIIKVDGSE
                     KNGQHKALNPNPYERVIPEGTLSKRIYQVNNLDDNQYGIELTVSGKTVYEQKDKSVPL
                     DVVILLDNSNSMSNIRNKNARRAERAGEATRSLIDKITSDSENRVALVTYASTIFDGT
                     EFTVEKGVADKNGKRLNDSLFWNYDQTSFTTNTKDYSYLKLTNDKNDIVELKNKVPTE
                     AEDHDGNRLMYQFGATFTQKALMKADEILTQQARQNSQKVIFHITDGVPTMSYPINFN
                     HATFAPSYQNQLNAFFSKSPNKDGILLSDFITQATSGEHTIVRGDGQSYQMFTDKTVY
                     EKGAPAAFPVKPEKYSEMKAAGYAVIGDPINGGYIWLNWRESILAYPFNSNTAKITNH
                     GDPTRWYYNGNIAPDGYDVFTVGIGINGDPGTDEATATSFMQSISSKPENYTNVTDTT
                     KILEQLNRYFHTIVTEKKSIENGTITDPMGELIDLQLGTDGRFDPADYTLTANDGSRL
                     ENGQAVGGPQNDGGLLKNAKVLYDTTEKRIRVTGLYLGTDEKVTLTYNVRLNDEFVSN
                     KFYDTNGRTTLHPKEVEQNTVRDFPIPKIRDVRKYPEITISKEKKLGDIEFIKVNKND
                     KKPLRGAVFSLQKQHPDYPDIYGAIDQNGTYQNVRTGEDGKLTFKNLSDGKYRLFENS
                     EPAGYKPVQNKPIVAFQIVNGEVRDVTSIVPQDIPAGYEFTNDKHYITNEPIPPKREY
                     PRTGGIGMLPFYLIGCMMMGGVLLYTRKHP"
     gene            3090..5087
                     /gene="rrgB"
                     /locus_tag="VVL45_001717"
     CDS             3090..5087
                     /gene="rrgB"
                     /locus_tag="VVL45_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus major pilin RrgB"
                     /protein_id="HEU4187860.1"
                     /translation="MKSINKFLTMLAALLLTASSLFSAATVFAAGTTTTSVTVHKLLA
                     TDGDMDKIANELETGNYAGNKVGVLPANAKEIAGVMFVWTNTNNEIIDENGQTLGVNI
                     DPQTFKLSGAMPATAMKKLTEAEGAKFNTANLPAAKYKIYEIHSLSTYVGEDGATLTG
                     SKAVPIEIELPLNDVVDAHVYPKNTEAKPKIDKDFKGKANPDTPRVDKDTPVNHQVGD
                     VVEYEIVTKIPALANYATANWSDRMTEGLAFNKGTVKVTVDDVALEAGDYALTEVATG
                     FDLKLTDAGLAKVNDQNAEKTVKITYSATLNDKAIVEVPESNDVTFNYGNNPDHGNTP
                     KPNKPNENGDLTLTKTWVDATGAPIPAGAEATFDLVNAQTGKVVQTVTLTTDKNTVTV
                     NGLDKNTEYKFVERSIKGYSADYQEITTAGEIAVKNWKDENPKPLDPTEPKVVTYGKK
                     FVKVNDKDNRLAGAEFVIANADNAGQYLARKADKVSQEEKQLVVTTKDALDRAVAAYN
                     ALTAQQQTQQEKEKVDKAQAAYNAAVIAANNAFEWVADKDNENVVKLVSDAQGRFEIT
                     GLLAGTYYLEETKQPAGYALLTSRQKFEVTATSYSATGQGIEYTAGSGKDDATKVVNK
                     KITIPQTGGIGTIIFAVAGAAIMGIAVYAYVKNNKDEDQLA"
     gene            5134..6315
                     /gene="rrgC"
                     /locus_tag="VVL45_001718"
     CDS             5134..6315
                     /gene="rrgC"
                     /locus_tag="VVL45_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus minor pilin RrgC"
                     /protein_id="HEU4187861.1"
                     /translation="MISRIFFVMALCFSLVWGAHAVQAQEDHTLVLQLENYQEVVSQL
                     PSRDGHRLQVWKLDDSYSYDDRVQIVRDLHSWDENKLSSFKKTSFEMTFLENQIEVSH
                     IPNGLYYVRSIIQTDAVSYPAEFLFEMTDQTVEPLVIVAKKTDTMTTKVKLIKVDQDH
                     NRLEGVGFKLVSVARDVSEKEVPLIGEYRYSSSGQVGRTLYTDKNGEIFVTNLPLGNY
                     RFKEVEPLAGYAVTTLDTDVQLVDHQLVTITVVNQKLPRGNVDFMKVDGRTNTSLQGA
                     MFKVMKEESGHYTPVLQNGKEVVVTSGKDGRFRVEGLEYGTYYLWELQAPTGYVQLTS
                     PVSFTIGKDTRKELVTVVKNNKRPRIDVPDTGEETLYILMLVAILLFGSGYYLTKKPN
                     N"
     gene            6653..7555
                     /gene="srtC-1"
                     /locus_tag="VVL45_001719"
     CDS             6653..7555
                     /gene="srtC-1"
                     /locus_tag="VVL45_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus system sortase SrtC-1"
                     /protein_id="HEU4187862.1"
                     /translation="MVKTKKQKRNNLLLGVVFFIGMAVMAYPLVSRLYYRVESNQQIA
                     DFDKEKATLDEADIDERMKLAQAFNDSLNNVVSGDPWSEEMKKKGRAEYARMLEIHER
                     MGHVEIPVIDVDLPVYAGTAEEVLQQGAGHLEGTSLPIGGNSTHAVITAHTGLPTAKM
                     FTDLTKLKVGDKFYVHNIKEVMAYQVDQVKVIEPTNFDDLLIVPGHDYVTLLTCTPYM
                     INTHRLLVRGHRIPYVAEVEEEFIAANKLSHLYRYLFYVAVGLIVILLWIIRRLRKKK
                     KQPEKALKALKAARKEVKVEDGQQ"
     gene            7542..8435
                     /gene="srtC-2"
                     /locus_tag="VVL45_001720"
     CDS             7542..8435
                     /gene="srtC-2"
                     /locus_tag="VVL45_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus system sortase SrtC-2"
                     /protein_id="HEU4187863.1"
                     /translation="MDNSRRSRKKGTKKKKHPLILLLIFLVGFAVAIYPLVSRYYYRI
                     ESNEVIKEFDETVSQMDKAELEERWRLAQAFNATLKPSEILDPFTEQEKKKGVSEYAN
                     MLKVHERIGYVEIPAIDQEIPMYVGTSEDILQKGAGLLEGASLPVGGENTHTVITAHR
                     GLPTAELFSQLDKMKKGDIFYLHVLDQVLAYQVDQIVTVEPNDFEPVLIQHGEDYATL
                     LTCTPYMINSHRLLVRGKRIPYTAPIAERNRAVRERGQFWLWLLLGAMAVILLLLYRV
                     YRNRRIVKGLEKQLEGRHVKD"
     gene            8422..9273
                     /gene="srtC-3"
                     /locus_tag="VVL45_001721"
     CDS             8422..9273
                     /gene="srtC-3"
                     /locus_tag="VVL45_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus system sortase SrtC-3"
                     /protein_id="HEU4187864.1"
                     /translation="MSRTKLRALLGYLLMLVACLIPIYCFGQMVLQSLGQVKGHATFV
                     KSMTTEMYQEQQNHSLAYNQRLASQNRIVDPFLAEGYEVNYQVSDDPDAVYGYLSIPS
                     LEIMEPVYLGADYHHLGMGLAHVDGTPLPLDGTGIRSVIAGHRAEPSHVFFRHLDQLK
                     VGDALYYDNGQEIVEYQMMDTEIILPSEWEKLESVSSKNIMTLITCDPIPTFNKRLLV
                     NFERVAVYQKSDPQTAAVARVAFTKEGQSVSRVATSQWLYRGLVVLAFLGILFVLWKL
                     ARLLRGK"
     gene            complement(9346..10622)
                     /locus_tag="VVL45_001722"
                     /pseudo
     CDS             complement(9346..10622)
                     /locus_tag="VVL45_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(10737..10886)
                     /locus_tag="VVL45_001723"
     CDS             complement(10737..10886)
                     /locus_tag="VVL45_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187865.1"
                     /translation="MDKMKPVFQALNKELIQENLTLTIICVGGYVLEYHGLRATQDVD
                     AFMAL"
     gene            complement(10879..11163)
                     /locus_tag="VVL45_001724"
     CDS             complement(10879..11163)
                     /locus_tag="VVL45_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187866.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
BASE COUNT         3901 a         1996 c         2763 g         3633 t
ORIGIN      
        1 aagtgtgtca ttgccaggtt gagaagatag ctataacgca cttttatacg cttttgctac
       61 gtttgttagt gaacggatta actcagtgag ataaatttta tcagaacata agtaatccgt
      121 ttcttcgtgt atacagattg aaagtaccta tgaatcatag aaggattaac ttgttctatg
      181 aataatgctt aacagggaga cacacatgaa aaaagtaaga aagatatttc agaaggcagt
      241 tgcaggactg tgctgtatat ctcagttgac agctttttct tcgatagttg ctttagcaga
      301 aacgcctgaa accagtccag cgataggaaa agtagtgatt aaggagacag gcgaaggagg
      361 agcgcttcta ggagatgccg tctttgagtt gaaaaacaat acggatggca caactgtttc
      421 gcaaaggaca gaggcgcaaa caggagaagc gatattttca aacataaaac ctgggacata
      481 caccttgaca gaagcccaac ctccagttgg ttataaaccc tctactaaac aatggactgt
      541 tgaagttgag aagaatggtc ggacgactgt ccaaggtgaa caggtagaaa atcgagaaga
      601 ggctctatct gaccagtatc cacaaacagg gacttatcca gatgttcaaa caccttatca
      661 gattattaag gtagatggtt cggaaaaaaa cggacagcac aaggcgttga atccgaatcc
      721 atatgaacgt gtgattccag aaggtacact ttcaaagaga atttatcaag tgaataattt
      781 ggatgataac caatatggaa tcgaattgac ggttagtggg aaaacagtgt atgaacaaaa
      841 agataagtct gtgccgctgg atgtcgttat cttgctcgat aactcaaata gtatgagtaa
      901 cattcgaaac aagaatgctc gacgtgcgga aagagctggt gaggcgacac gttctcttat
      961 tgataaaatt acatctgatt cagaaaatag ggtagcgctt gtgacttatg cttccactat
     1021 ctttgatggg accgagttta cagtagaaaa aggggtagca gataaaaacg gaaagcgatt
     1081 gaatgattct cttttttgga attatgatca gacgagtttt acaaccaata ccaaagatta
     1141 tagttattta aagctgacta atgataagaa tgacattgta gaattaaaaa ataaggtacc
     1201 taccgaggca gaagaccatg atggaaatag attgatgtac caattcggtg ccacttttac
     1261 tcagaaagct ttgatgaagg cagatgagat tttgacacaa caagcgagac aaaatagtca
     1321 aaaagtcatt ttccatatta cggatggtgt cccaactatg tcgtatccga ttaattttaa
     1381 tcatgctacg tttgctccat catatcaaaa tcaactaaat gcatttttta gtaaatctcc
     1441 taataaagat ggaatactat taagtgattt tattacgcaa gcaactagtg gagaacatac
     1501 aattgtacgc ggagatgggc aaagttacca gatgtttaca gataagacag tttatgaaaa
     1561 aggtgctcct gcagctttcc cagttaaacc tgaaaaatat tctgaaatga aggcggctgg
     1621 ttatgcagtt ataggcgatc caattaatgg tggatatatt tggcttaatt ggagagagag
     1681 tattctggct tatccgttta attctaatac tgctaaaatt accaatcatg gtgaccctac
     1741 aagatggtac tataacggga atattgctcc tgatgggtat gatgtcttta cggtaggtat
     1801 tggtattaac ggagatcctg gtacggatga agcaacggct actagtttta tgcaaagtat
     1861 ttctagtaaa cctgaaaact ataccaatgt tactgacacg acaaaaatat tggaacagtt
     1921 gaatcgttat ttccacacca tcgtaactga aaagaaatca attgagaatg gtacgattac
     1981 agatccgatg ggtgagttaa ttgatttgca attgggcaca gatggaagat ttgatccagc
     2041 agattacact ttaactgcaa acgatggtag tcgcttggag aatggacaag ctgtaggtgg
     2101 tccacaaaat gatggtggtt tgttaaaaaa tgcaaaagtg ctctatgata cgactgagaa
     2161 aaggattcgt gtaacaggtc tgtaccttgg aacggatgaa aaagttacgt tgacctacaa
     2221 tgttcgtttg aatgatgagt ttgtaagcaa taaattttat gataccaatg gtcgaacaac
     2281 cttacatcct aaggaagtag aacagaacac agtgcgcgac ttcccgattc ctaagattcg
     2341 tgatgtgcgg aagtatccag aaatcacaat ttcaaaagag aaaaaacttg gtgacattga
     2401 gtttattaag gtcaataaaa atgataaaaa accactgaga ggtgcggtct ttagtcttca
     2461 aaaacaacat ccggattatc cagatattta tggagctatt gatcaaaatg gcacttatca
     2521 aaatgtgaga acaggtgaag atggtaagtt gacctttaaa aatctgtcag atgggaaata
     2581 tcgattattt gaaaattctg aaccagctgg ttataaaccc gttcaaaata agcctatcgt
     2641 tgccttccaa atagtaaatg gagaagtcag agatgtgact tcaatcgttc cacaagatat
     2701 accagcgggt tacgagttta cgaatgataa gcactatatt accaatgaac ctattcctcc
     2761 aaagagagaa tatcctcgaa ctggtggtat cggaatgttg ccattctatc tgataggttg
     2821 catgatgatg ggaggagttc tattatacac acggaaacat ccgtaaagtg tagaaatgat
     2881 aatatctatg ttctgaacga tacttttaag aagtagcact caagaagaga tttaagttta
     2941 cttggtgaaa cctgttttat tcgtaagtaa actatcattg aaaggggaga tgttttcgaa
     3001 aacttgcaca gaaaaaggat tattattgtc atgtgtaatt cattacattg ctcacagttg
     3061 attttaagag atatgaataa ggagaaatca tgaaatcaat caacaaattt ttaacaatgc
     3121 ttgctgcctt attactgaca gcgagtagcc tgttttcagc tgcaacagtt tttgcggctg
     3181 ggacgacaac aacatctgtt accgttcata aactattggc aacagatggg gatatggata
     3241 aaattgcaaa tgagttagaa acaggtaact atgctggtaa taaagtgggt gttctacctg
     3301 caaatgcaaa agaaattgcc ggtgttatgt tcgtttggac aaatactaat aatgaaatta
     3361 ttgatgaaaa tggccaaact ctaggagtga atattgatcc acaaacattt aaactctcag
     3421 gggcaatgcc ggcaactgca atgaaaaaat taacagaagc tgaaggagct aaatttaaca
     3481 cggcaaattt accagctgct aagtataaaa tttatgaaat tcacagttta tcaacttatg
     3541 tcggtgaaga tggagcaacc ttaacaggtt ctaaagcagt tccaattgaa attgaattac
     3601 cattgaacga tgttgtggat gcgcatgtgt atccaaaaaa tacagaagca aagccaaaaa
     3661 ttgataaaga tttcaaaggt aaagcaaatc cagatacacc acgtgtagat aaagatacac
     3721 ctgtgaacca ccaagttgga gatgttgtag agtacgaaat tgttacaaaa attccagcac
     3781 ttgctaatta tgcaacagca aactggagcg atagaatgac tgaaggtttg gcattcaaca
     3841 aaggtacagt gaaagtaact gttgatgatg ttgcacttga agcaggtgat tatgctctaa
     3901 cagaagtagc aactggtttt gatttgaaat taacagatgc tggtttagct aaagtgaatg
     3961 accaaaacgc tgaaaaaact gtgaaaatca cttattcggc aacattgaat gacaaagcaa
     4021 ttgtagaagt accagaatct aatgatgtaa catttaacta tggtaataat ccagatcacg
     4081 ggaatactcc aaagccgaat aagccaaatg aaaacggcga tttgacattg accaagacat
     4141 gggttgatgc tacaggtgca ccaattccgg ctggagctga agcaacgttc gatttggtta
     4201 atgctcagac tggtaaagtt gtacaaactg taactttgac aacagacaaa aatacagtta
     4261 ctgttaacgg attggataaa aatacagaat ataaattcgt tgaacgtagt ataaaagggt
     4321 attcagcaga ttatcaagaa atcactacag ctggagaaat tgctgtcaag aactggaaag
     4381 acgaaaatcc aaaaccactt gatccaacag agccaaaagt tgttacatat ggtaaaaagt
     4441 ttgtcaaagt taatgataaa gataatcgtt tagctggggc agaatttgta attgcaaatg
     4501 ctgataatgc tggtcaatat ttagcacgta aagcagataa agtgagtcaa gaagagaagc
     4561 agttggttgt tacaacaaag gatgctttag atagagcagt tgctgcttat aacgctctta
     4621 ctgcacaaca acaaactcag caagaaaaag agaaagttga caaagctcaa gctgcttata
     4681 atgctgctgt gattgctgcc aacaatgcat ttgaatgggt ggcagataag gacaatgaaa
     4741 atgttgtgaa attagtttct gatgcacaag gtcgctttga aattacaggc cttcttgcag
     4801 gtacatatta cttagaagaa acaaaacagc ctgctggtta tgcattacta actagccgtc
     4861 agaaatttga agtcactgca acttcttatt cagcgactgg acaaggcatt gagtatactg
     4921 ctggttcagg taaagatgac gctacaaaag tagtcaacaa aaaaatcact atcccacaaa
     4981 cgggtggtat tggtacaatt atctttgctg tagcgggggc tgcgattatg ggtattgcag
     5041 tgtacgcata tgttaaaaac aacaaagatg aggatcaact tgcttaagta agagagaaag
     5101 gagccattga tgacaatgca gaaaatgcag aaaatgatta gtcgtatctt ctttgttatg
     5161 gctctgtgtt tttctcttgt atggggtgca catgcagtcc aagcgcaaga agatcacacg
     5221 ttggtcttgc aattggagaa ctatcaggag gtggttagtc aattgccatc tcgtgatggt
     5281 catcggttgc aagtatggaa gttggatgat tcgtattcct atgatgatcg ggtgcaaatt
     5341 gtaagagact tgcattcgtg ggatgagaat aaactttctt ctttcaaaaa gacttcgttt
     5401 gagatgacct tccttgagaa tcagattgaa gtatctcata ttccaaatgg tctttactat
     5461 gttcgctcta ttatccagac ggatgcggtt tcttatccag ctgaatttct ttttgaaatg
     5521 acagatcaaa cggtagagcc tttggtcatt gtagcgaaaa aaacagatac aatgacaaca
     5581 aaggtgaagc tgataaaggt ggatcaagac cacaatcgct tggagggtgt cggctttaaa
     5641 ttggtatcag tagcaagaga tgtttctgaa aaagaggttc ccttgattgg agaataccgt
     5701 tacagttctt ctggtcaagt agggagaact ctctatactg ataaaaatgg agagattttt
     5761 gtgacaaatc ttcctcttgg gaactatcgt ttcaaggagg tggagccact ggcaggctat
     5821 gctgttacga cgctggatac ggatgtccag ctggtagatc atcagctggt gacgattacg
     5881 gttgtcaatc agaaattacc acgtggcaat gttgacttta tgaaggtgga tggtcggacc
     5941 aatacctctc ttcaaggggc aatgttcaaa gtcatgaaag aagaaagcgg acactatact
     6001 cctgttcttc aaaatggtaa ggaagtagtt gtaacatcag ggaaagatgg tcgtttccga
     6061 gtggaaggtc tagagtatgg gacatactat ttatgggagc tccaagctcc aactggttat
     6121 gttcaattaa catcgcctgt ttcctttaca atcgggaaag atactcgtaa ggaactggta
     6181 acagtggtta aaaataacaa gcgaccacgg attgatgtgc cagatacagg ggaagaaacc
     6241 ttgtatatct tgatgcttgt tgccattttg ttgtttggta gtggttatta tcttacgaaa
     6301 aaaccaaata actgatattc aatgtacatc attatgaaaa agatagcagg ctgaagggaa
     6361 gaccagagta ctctgaggtg atgttaatca ggaatcatgg tgatgtggca tgaatcacaa
     6421 taacggatat gaggctgggc agattgtgcc agcctcattg tgggttattg tttgtaaaac
     6481 gataggactg gtctggtaat cattttagga atggacagga ctgggattct gatttaaaat
     6541 ggatggtgaa tcagaaagaa atgagatttt ctcgtttctc ttagcagata ggattgtctg
     6601 ttaggaaaag cgataaaatg atgagtttga agataaaggg atgctgataa aaatggtaaa
     6661 aacaaaaaag caaaaacgaa ataatctcct attaggagtg gtatttttca ttggaatggc
     6721 ggtaatggcg tatccgctgg tgtctcgctt gtattatcga gtggaatcaa atcaacaaat
     6781 tgctgacttt gataaggaaa aagcaacgtt ggatgaggct gacattgatg aacgaatgaa
     6841 attggcacaa gccttcaatg actctttgaa taatgtagtg agtggcgatc cttggtcgga
     6901 agaaatgaag aaaaaagggc gagcagagta tgcacgtatg ttagaaatcc atgagcggat
     6961 ggggcatgtg gaaatccccg ttattgacgt ggatttgccg gtttatgctg gtactgctga
     7021 agaggtattg cagcaagggg ctgggcatct agagggaact tctctgccga tcggaggcaa
     7081 ttcgacccat gcggtgatta cggcacatac aggtttgcca acagctaaga tgtttacgga
     7141 tttgaccaaa cttaaagttg gggataagtt ttatgtgcac aatatcaagg aagtgatggc
     7201 ctatcaagtg gatcaagtaa aggtgattga gccgacgaac tttgatgatt tattgattgt
     7261 accaggtcat gattatgtga ccttgctgac ttgtacgcca tacatgatca atacccatcg
     7321 tctattggtt cgggggcatc ggataccgta cgtagcagag gttgaggaag aatttattgc
     7381 agcaaacaaa ctcagtcatc tctatcgcta cctgttttat gtggcagttg gtttgattgt
     7441 gattctttta tggattattc gacgcttgcg caagaagaaa aaacaaccgg aaaaggcttt
     7501 gaaggcgctg aaagcagcaa ggaaggaagt gaaggtggag gatggacaac agtagacgtt
     7561 cacgaaaaaa aggcacaaaa aagaagaaac atccgctgat ccttcttctg attttcttag
     7621 taggattcgc cgttgcgata tatccattgg tgtctcgtta ttattatcgt attgagtcaa
     7681 acgaggttat taaagagttt gatgagacgg tttcccagat ggataaggca gaacttgagg
     7741 agcgttggcg cttggctcaa gccttcaatg cgaccttgaa accatctgaa attcttgatc
     7801 cttttacaga gcaagagaaa aagaaaggcg tctcagaata tgccaatatg ctaaaggtcc
     7861 atgagcggat tggctatgtg gaaattcctg cgattgatca ggaaattccg atgtatgtcg
     7921 gaacgagtga ggacattctt cagaaagggg cagggctgtt agaaggggct tcgctgcctg
     7981 ttggaggtga aaatacccat acagtgatca ctgctcacag aggattgcca acggcagaat
     8041 tgttcagtca attggataag atgaaaaaag gggatatctt ttatcttcac gttttagatc
     8101 aggtgttggc ctaccaagtg gatcagatag tgacggtgga gccgaatgac tttgagcctg
     8161 tcttgattca acatggggaa gattatgcga ccttgttgac ttgtacaccg tatatgatta
     8221 acagtcatcg tctgttggta cgtgggaagc ggattccgta tacggcacca attgcagagc
     8281 ggaatcgagc ggtgagagag cgtgggcaat tctggttgtg gttattacta ggagcgatgg
     8341 cggtcatcct tctcttgctg tatcgcgtgt atcgtaatcg acggattgtc aaaggactag
     8401 aaaagcaatt ggaggggcgt catgtcaagg actaaactac gagccttatt gggatacttg
     8461 ttgatgttgg tagcctgttt gattcctatt tattgttttg gacagatggt gttgcagtct
     8521 cttggacagg tgaaaggtca tgctacattt gtgaaatcca tgacaactga aatgtaccaa
     8581 gaacaacaga accattctct cgcctacaat caacgcttgg cttcgcaaaa tcgcattgta
     8641 gatccttttt tggcggaggg atatgaggtc aattaccaag tgtctgacga ccctgatgca
     8701 gtctatggtt acttgtctat tccaagtttg gaaatcatgg agccggttta tttgggagca
     8761 gattatcatc atttagggat gggcttggct catgtggatg gtacaccgct gcctctggat
     8821 ggtacaggga ttcgctcagt gattgctggg caccgtgcag agccaagcca tgtctttttc
     8881 cgccatttgg atcagctaaa agttggagat gctctttatt atgataatgg ccaggaaatt
     8941 gtagaatatc agatgatgga cacagagatt attttaccgt cggaatggga aaaattagaa
     9001 tcggttagct ctaaaaatat catgaccttg ataacctgcg atccgattcc tacctttaat
     9061 aaacgcttat tagtgaattt tgaacgagtc gctgtttatc aaaaatcaga tccacaaaca
     9121 gctgcagttg cgagggttgc ttttacgaaa gaaggacaat ctgtatcgcg tgttgcaacc
     9181 tctcaatggt tgtaccgtgg gctagtggta ctggcatttc tgggaatcct gtttgttttg
     9241 tggaagctag cacgtttact acgagggaaa taaaaagaaa tgaaaggaaa gctaaggctg
     9301 ttcctttttc cggctctttg tcaactgtag tgggttgaaa aaaagctaag ctcgagaaag
     9361 gacaaatttt gtcctttctt ttttgatatt cagagcgata aaaatccgtt ttttgaagtt
     9421 ttcaaagttt cgaaaaccaa aggcattgcg cttgataagt ttgatgagat tattggtcgc
     9481 ttccagtttg gcattagaat agtgtagttg aagggcgttg ataacctttt ctttatcttt
     9541 gaggaaggtt ttaaagacag tctgaaaaat aggatgaacc tgcttaagat tgtcctcaat
     9601 aagtccgaaa aatttctccg gttccttatt ctgaaagtga aacagcaaga gttgatagag
     9661 ctgatagtga tgtttcaagt cttgtgaata gctcaaaagc ttgtctaaaa tctctttatt
     9721 ggttaaatgc atacgaaaag tagggcgata aaaatgttta tcgctgagtt tacggctatc
     9781 ctgttgaatg agtttccagt agcgcttgat agccttgtat tcatgggatt ttcgatgaaa
     9841 ctgattcatg atttggatac gcacacgact catagcacgg ctaagatgtt gtacaatgtg
     9901 aaagcgatca agaacgattt tagcacacgg gaaaagttgt ctagctaagt catagtaagg
     9961 actaaacata tccatagtaa tgattttgac gcgacatcgg acggctctat catatttaag
    10021 aaagtgatct cggatgacag cttgtgttct accttcaaga acagtgatga tatcgagctt
    10081 ttcaaaatct tgcgcaataa agctcatctc catctcccga ttgaaacagt cactccccgg
    10141 actgtttcaa cgtcccagga cataatctca ggaagacgcg aaaaatcatg ctcaaagtga
    10201 gaatcattga gcttgcgaat gacagttgaa gttgaaatgg acagctgatg agcaatatcg
    10261 gtcatagaaa tcttctcaat caacttttgc gcaatttttt ggttgataat acgaggaatt
    10321 tgatgattct tcttgacgat agaagtttca gcgaccatca tttttgaaca gtgatagcac
    10381 ttgaaacggc gttttctaag gagaattcta gaaggcatac cagttgtttc gaggtaaggg
    10441 atcttagacg gtttttgaaa gtcatatttc ttcaattggt ttccgcactc agggcaagat
    10501 ggggcgtcgt agtccagttt ggcgatgatt tccttgtgtg tatccttatt gatgatgtct
    10561 aaaatctgga tattagggtc tttaatgtct agtaattttg tgataaaatg taattgttcc
    10621 atatgattct ttctaatgag ttgttttgtc gcttttcatt ataggtcata tgggactttt
    10681 tttctacaca aaaataggct ccataatatc cataggggat ttacccacta caaatattat
    10741 agagccataa aagcatcaac atcttgtgtg gcacgtaaac catgatattc taagacataa
    10801 ccaccgacac agataattgt taaagtcaga ttttcctgaa ttaattcctt atttagggct
    10861 tggaagaccg gtttcatttt atccatgttt gtatttctcc atcaattttt gaacattggt
    10921 taacttagca ggtttattta atgaaatcac atatttatga gttggagtat gctcttgaaa
    10981 ggcatacttc aagctaattt tttgaaaacg ggttagttct ttggagtcta gcaacttttc
    11041 tacatcttgg tttgttaaat aagaatagtc ttcaaaaata acttttcgca aatagcgaaa
    11101 ccaatggctt gcgttgaatt gtgattgttt ggatgcctgt atcatataat cgtaccaatc
    11161 cattgtcttc ctcctataaa atttagttta tattattgta gtttatattc aaaaatgtag
    11221 tttttcttaa ttagctcgta tctataggat gatactgaaa atcttcttgt tgctttaaat
    11281 cacaatcgaa aaattctgtt tttcatctta gaaaagaatg tttagaaatg taaaatgaac
    11341 cttttttctt atccgaaaac cttactaaag tatataattt aggaagtcaa tcctgtatga
    11401 taatgtttgt actatcgtcg agtttttgct tgattgccta acgagataaa ttttagaaga
    11461 attttcggtg tgatttgaca agcttctgac tggaaggata gttacactag tattgtttat
    11521 taaaatgagc agatagagaa aatttggaat ctatttgctt attatcaatc gtttttttag
    11581 cttatttgtc ttagattggt atagaactga gctatggatt aatactcttg tatcttatcc
    11641 tgttcctaaa tatgttggta gaaaaatgaa gcagttgtta cgagtgtctg tattggaaaa
    11701 aattttgtct atagaattct ttaaatgata tgaaggctag gaatgccaaa gcactgacat
    11761 atttcgtttg aatctcatcc gctctcttta cttgtatttg tagaaatgct atcagtaata
    11821 tattcgcgaa cactatgatt tacaacagtg tggtcgtaca tctttgtaac tttattatac
    11881 aacaaccatt tacaggtgta aagttgagca gtgtctttag taaatttagg gatttgtttt
    11941 gtcatagttt tgatacctgt attcgttgtt tcatgagcat ttgacgtttc ttttcatgta
    12001 ggcatttcaa caattagaat gatatttttc cgaggatgaa cttatatatt gtgacttttt
    12061 agagttatga tgcctcttga tttgactatc gctatcaatt tcatccactt ttacagtttc
    12121 atggttttat gaaccgaaac atctaggtac aggttttagc atcaattgat tgtttttgag
    12181 ccatatgaag ttgaaacgtg gagatgcttt tcttgtgata acgccctatt caaagaggaa
    12241 taaccgtttt tggagagagt tcagtactat gtggcgctaa atactatatg taa
//
LOCUS       DAVHEX010000058        12085 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVHEX010000058 DAVHEX010000000
VERSION     DAVHEX010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12085
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.058"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            23..214
                     /locus_tag="VVL45_001725"
     CDS             23..214
                     /locus_tag="VVL45_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187867.1"
                     /translation="MYFRFFSNFDDFLEKNYLKTIWQVSKETPFSEMDFNMFKNISEK
                     IIFERGSKMLNDLKSNYKK"
     gene            352..1005
                     /locus_tag="VVL45_001726"
     CDS             352..1005
                     /locus_tag="VVL45_001726"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU4187868.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            1015..1479
                     /locus_tag="VVL45_001727"
     CDS             1015..1479
                     /locus_tag="VVL45_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU4187869.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            1492..2760
                     /locus_tag="VVL45_001728"
     CDS             1492..2760
                     /locus_tag="VVL45_001728"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEU4187870.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIASEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLS
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRFVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            2774..3034
                     /locus_tag="VVL45_001729"
     CDS             2774..3034
                     /locus_tag="VVL45_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187871.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            3045..4406
                     /gene="pdrM"
                     /locus_tag="VVL45_001730"
     CDS             3045..4406
                     /gene="pdrM"
                     /locus_tag="VVL45_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HEU4187872.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPIILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDKIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSPLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTVRFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKC"
     gene            4560..5528
                     /locus_tag="VVL45_001731"
     CDS             4560..5528
                     /locus_tag="VVL45_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEU4187873.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            5544..6536
                     /locus_tag="VVL45_001732"
     CDS             5544..6536
                     /locus_tag="VVL45_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEU4187874.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDNNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVAYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
     gene            6754..7797
                     /locus_tag="VVL45_001733"
     CDS             6754..7797
                     /locus_tag="VVL45_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEU4187875.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGKIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            7861..9546
                     /gene="lpdA"
                     /locus_tag="VVL45_001734"
     CDS             7861..9546
                     /gene="lpdA"
                     /locus_tag="VVL45_001734"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEU4187876.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQFGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVNKINVPGMESPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDVEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            9610..10599
                     /locus_tag="VVL45_001735"
     CDS             9610..10599
                     /locus_tag="VVL45_001735"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEU4187877.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINRIKDTKFGTWDWNYGKSPEFNVRRGIKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(10664..11734)
                     /gene="xerS"
                     /locus_tag="VVL45_001736"
     CDS             complement(10664..11734)
                     /gene="xerS"
                     /locus_tag="VVL45_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEU4187878.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGCKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVSDEQKNALDSL"
BASE COUNT         3605 a         2238 c         2694 g         3548 t
ORIGIN      
        1 gattttacac caagagaatg cattgtattt tcgatttttt tctaattttg acgacttttt
       61 agaaaaaaat tatttaaaga ctatatggca agtttctaaa gaaactcctt tttctgagat
      121 ggattttaat atgtttaaaa atatttcaga aaagataatc ttcgaaagag gtagtaaaat
      181 gcttaatgat ttaaaaagta attataaaaa atagccagtc tttataaact gtattcacgt
      241 attcaaatcg tatatatagt cgatttatta aatctttaga taaactgtaa aaagtggttt
      301 ccatagccac tttttgctat aatagaggca gtaaaaatga aggagtcggc tatggaacac
      361 tcgtcttggc atgctttgat taaggcgcaa ttacctgagg gttatttcgg gaaaatcaat
      421 cagtttatgg agcaggtcta ttctcagggg attatttatc cacccaagga aaaggttttt
      481 caggctctct tgacaacact gcttgaagaa gttaaggtgg taattctagg gcaagacccc
      541 tatcacggac caggtcaagc gcagggcttg agtttttctg tacctgactc tatcccagct
      601 ccaccatcct tgcaaaatat cttgaaagaa ttgtcagatg atatcggagt taagaaatct
      661 catgatttga cagcttgggc tgagcaagga gtcttgcttc ttaatgcttg tttgactgtt
      721 cctgctggac aggccaatgg tcatgctggt caaatctggg agccttttac tgatgctgtg
      781 attcaggtgg tcaatcatct agatagacca gtcgtttttg tactctgggg agcttatgca
      841 cgtaagaaga aggcattagt taccaatcct catcacttga ttatcgaatc agcccatcca
      901 agtcctttgt cggtttatag aggattttgg ggttccaagc ccttttccaa ggccaatgca
      961 ttcttaaaag agacaggaca agagccaatc gattggctta gataaggaga aaatatgcct
     1021 cagttagcga cgatttgcta cattgataat gggaaagaac tgctcatgct ccatcgtaat
     1081 aagaaaccca atgatgtcca tgaagggaaa tggattggtg tgggtggtaa gctagagaga
     1141 ggagagacgc cccaggaatg cgcggcgcgt gaaatccttg aagaaacagg gctcaaagcc
     1201 aagccagttc taaaaggtgt catcactttt cctgaattta caccagattt agactggtac
     1261 acctatgttt ttaaggtgac ggagtttgag ggcgacttga ttgactgcaa tgaggggacg
     1321 ctagaatggg ttccctatga tgaggttttg agcaagccga cttgggaagg tgaccacacc
     1381 tttgttgagt ggcttttaga ggataaaccc ttcttttcag ccaagtttgt ttatgatggg
     1441 gataaattgt tggataccca agttgatttc tatgaataaa ggagaaagca gatgctacta
     1501 atcaaaaatg gtcgtgtaat ggatcccaag tctggtttgg atcaagtttg tgatgtctta
     1561 gttcaagatg ggaaaattat caaaattgcg tctgagatca cggaagaagg agcagaaacg
     1621 attgatgcta ctggtcttgt ggttgctcct ggcttggtcg atattcatgt tcatttccgt
     1681 gaacctggtc aaacacataa agaagacatt catactggtg ccctagcagc cgctgcaggt
     1741 ggttttacta ctgtcgtcat gatggctaat accagtccaa ccatttcaga cgtggagact
     1801 ttgcaagcag ttctccagtc agctgccaaa gagaagatta atgtcaagac agttgcgacc
     1861 attactaaaa actttaatgg taaaaacttg actgacttta aggcactctt agaagctggt
     1921 gcggttggtt tctctgatga cggtattccg cttgagagca gtaagattgt caaggaagcc
     1981 atggaggaag ccaaaaaact caataccttt atcagccttc atgaggaaga tccaggtttg
     2041 aacggtgttc ttggctttaa tgaaaatatt gctagagaac atttccatat ctgcggtgct
     2101 actggggtgg ctgagtacgc tatgatggcg cgtgatgtca tgattgccta tgcaactaaa
     2161 gcccatgttc acatccagca tttgtctaag gaagaaagtg ttaaagtagt ggagtttgct
     2221 caggggttag gtgcagaagt cacagcagaa gtagcgccac agcatttctc taagacagaa
     2281 gcacttcttt taacacaagg tagcaatgct aagatgaatc caccgcttcg tttggaatca
     2341 gaccgtcgtg ccgttatcga aggtctcaaa tcaggtgtca tcacagttat tgcgactgac
     2401 cacgcgcctc atcatgtaga tgaaaaaaat gttgaggata ttaccaaagc gccatctggt
     2461 atgactggct tagaaacatc cctgtctctc agcttgacct atttagtaga agctggtgag
     2521 ttgagcttga tggaattact tgaaaaaatg acatacaacc cagccaagct ttacaacttt
     2581 gaagcaggtt acttggctga gaatggtcca gcagatatca ctatttttga tgccaaggct
     2641 gaccgctttg tggactccca ttttgcttcc aaagcagcta attcaccatt catcggtgaa
     2701 accttaaaag ggcaggttaa atataccatc tgtaagggac aaatcgtcta tcaagcttga
     2761 taggagttag aatatgaatt attttcgaaa acgtagggag agacaagcga aatctaatag
     2821 tggaatttac tgtcccgcag ctaatcgttc caaaattcaa tatgaaacta aagaaaaagc
     2881 tgaccatgcg gttcaatatg ataacggagg tttagttaga agctattatt gtcgtacatg
     2941 tgcgtgttgg catactactt caaaatctaa taaacctccg ctaagtttga aaaactatgg
     3001 tctgaaactc tttggtttag ataaatcaga atagaaagag aagtatgtat aagacaaagt
     3061 gtttacgaga gaagttagta ttatttttaa aaattttctt cccaatcctg atctaccaat
     3121 ttgccaatta ttctgcctct tttgttgata ctgcaatgac aggtcaatac aacactatgg
     3181 acttggctgg tgtatctatg gcaaccagta tctggaatcc tttctttaca tttctaacag
     3241 ggattgtgtc agccttggtg cctatcattg gtcaccatct tggtcgaggc aaaaaggaag
     3301 aagttgcgtc tgatttttac caatttattt atttggcctt gggcctatct gtggtcttgc
     3361 tggggatggt acttttcttg gcaccaataa tcttgaatca tattgggtta gaagcagcag
     3421 tagcggcagt agcggttcgc tatctttggt ttttatctat cgggattatc cccttgttgc
     3481 tctttagcgt cattcgttcc ttgctggatt cgctgggctt gaccaaactg tccatgtacc
     3541 tcatgctttt gttactccct ctcaatagcg gatttaacta tctcttgatt tacggtgcct
     3601 ttggtgttcc agaactggga ggggctggtg ctggtttagg aacatccttg gcctactggg
     3661 tcttgcttgg gatttctgtt ctggttttat ttaaacagga gaagctcaaa gccttacacc
     3721 ttgagaaacg aattccactt aatatggata aaattaagga aggagttcgt ttaggtctgc
     3781 ctattggggg aactgtcttc gcggaagtgg ctatcttttc agtggttggc ttgattatgg
     3841 ctaagttttc gcccttgatt atagctagtc accagtcagc tatgaacttt tcaagtctta
     3901 tgtacgcctt tcctatgagt atctcatcgg ctatggctat tgtcgtttcc tatgaagtgg
     3961 gagccaagcg atttgatgat gcgaaaacct atattggtct aggaagatgg actgccctca
     4021 tttttgcggc cttcacctta accttccttt acatttttag gggaaatgtg gccagtcttt
     4081 atggtaacga cccaaaattt atcgatttga cagtgcgttt tttaacttat agtcttttct
     4141 tccagttagc agataccttt gcggcgccgc ttcagggaat tttgcggggg tataaggata
     4201 cagttattcc tttttacctt ggtttgcttg gttattgggg cgtagcaatc cctgtggcta
     4261 cgctatttga ttccctaaca gattttggag cttattctta ctggatcggc ttgattatta
     4321 gtttgattgt gagcggggcg ctctaccgtt ggcgtttaac tgtgattatg aagagatttg
     4381 aatctttagc aaaatccaaa tgctaaaaag tttgtgaaaa aatattcttg acaagaaaat
     4441 cccttgtttt cacggggttt tcttttttat tttgtgaaat ttcttttaga aaaactttga
     4501 cagtatttgc caaaagcggt aaaatagtaa ggtaagaaga aagttagaaa ggcaagaaga
     4561 tgtcaacttt agataaaaat cttttgctag aaatgttccg taagatggaa gaaatccgtc
     4621 gcatggactt aaaaattgca caattagtaa agaaagggaa agtgccagga atgacgcact
     4681 tttctgttgg tgaagaggca gctaacgtgg gggctatgtt agctctcaat ccagatgatc
     4741 tgattacctc aaaccaccgt ggacacgggc aagctattgc taaagggatt gacctcaacg
     4801 gaatgatggc tgaaatcctt ggtaaataca ctggaacctg taaagggaaa ggtggatcta
     4861 tgcatatcgc tgaccttgat gctgggaacc ttggtgccaa tggtatcgta ggtggtggta
     4921 tggggatcgc tgtcggtgca gccctcagtc agcaaatgca aaataccggt aaaatcgttg
     4981 tctgcttctt tggagatggt gcgaccaatg aaggtgtttt ccacgaagca gtgaacatgg
     5041 cttctatctg gaacctgcca gtcattttct attgcattaa caacggttac ggtatctctg
     5101 cggatatcaa gaaaatgacc aatatagaac atatccatca acgtagcgcc gcttatggaa
     5161 ttcctggaat gttcatcgaa gacggtaaca atgtcatcga tgtctatgaa ggatttcaga
     5221 aagctgtgga ccatgttcgc agtggcaatg gtccagtctt gattgaaagt gtaacttatc
     5281 gctggctcgg tcactcatca tctgaccctg gtaaatatcg tacgcgtgaa gaagtggaat
     5341 tgtggaaaca aaaagatcca atcgaaaacc tccgcaatta ccttattgaa aataacattg
     5401 caagtgccga agaattggaa gaaatccaag cgcaagtaaa ggaagcagta gaagcttctg
     5461 ttaaatttgc agaggaaagc ccattcccac cgcttgaatc agcctttgaa gatatttacg
     5521 cagactaagg agaaagagat aaaatggaaa caaaaacaat gtccttccgt gacaccatta
     5581 tccttgctat gtctgaggaa atgcgtcgcg atgaaaatgt gttcttgatg ggagaagacg
     5641 tcggtgtctt cggaggagac ttcggtactt ctgttggaat gcttgaagaa tttggtccag
     5701 aacgtgtccg tgactgtccg atttctgaag ctgccatctc tggagcagca gcaggagcag
     5761 ccatgacagg acttcgtcca atcgtcgata tgaccttcat ggacttctct gttattgcca
     5821 tggacaatat cgtcaaccaa gctgctaaaa cacgttacat gtttggtggt aaaggtcagg
     5881 ttccaatgac tgttcgttgt gcagctggta acggagttgg ctctgccgcc cagcactcac
     5941 aatctctaga gtcttggttt actcacattc caggacttaa ggttgtggca cctggaacac
     6001 ctgcggacat gaaaggcctg cttaagtctt ctatccgtga taacaaccca gttattatac
     6061 ttgagtacaa gtcagaattt aaccaaaaag gggaagtgcc agttgatcca gactacacaa
     6121 ttccacttgg ggttggtgaa atcaaacgcc aaggaacgga tgtaacagtt gttgcttatg
     6181 gaaaaatgct tcgccgtgtg gttcaagctg ctgaagaatt agcagaagaa ggaatttcag
     6241 ttgaaattgt tgacccacgt acccttgttc cgcttgataa ggatatcatc ataaactcag
     6301 tgaagaagac tggtaaggtt gttctggtca acgatgccca caaaacaagt ggctatatcg
     6361 gtgaaatttc agctattatt tcagaatcag aagcatttga ctatctagat gcaccaattc
     6421 gccgttgtgc aggagaagat gtgccgatgc cttatgcaca aaacctagaa aatgcaatga
     6481 ttccaacagt tgaaagtatc aaagatgcaa tccgaaaaac ttataacaaa gaatagggtt
     6541 acataagata agttatgtaa ataaaataaa gctgacgaga aactttgtat cttttaggtg
     6601 cagagctctt cgtcattaat atcttagatt agagcacggg ctaaaagatc ggaaaaaaga
     6661 taaatctcct tggcttcatc gaagccatcg tcgatttcct atttttcagt cgcttttgac
     6721 gccctttgta tcataaatag aattggagag gtcatggctg atgacaagct aagagcgact
     6781 cctgcagcta gaaagttagc ggatgattta gggataaacc tctacgacgt ttctggctca
     6841 ggtgcaaacg gtcgtgtcca caaagaagac gtggaaactt ataaagacac aaacgtggtt
     6901 cgcatttcgc cacttgcaaa acgaattgcc ctcgaacata acattgcttg gcaggaaatc
     6961 caaggaaccg gtcatcgtgg taaaatcatg aagaaggatg ttttggccct gcttcctgaa
     7021 aatatcgaaa acgatagcat caaatctcct gctcagattg aaaaagtgga agaagtccct
     7081 gataacgtaa caccatatgg taaaatcgag cgtattccaa tgacaccaat gcgtaaggtt
     7141 attgcccaac gcatggttga atcttacttg actgcgccaa ccttcaccct caactatgaa
     7201 gttgatatga ctgaaatgtt ggctcttcgt aagaaggttc ttgagccaat catggaagca
     7261 actgggaaga agactactgt aacagacctt ctttcgcttg cagttgttaa gactcttatg
     7321 aaacacccat acatcaacgc ttcattgaca gaagatggca agactattat cactcacaac
     7381 tatgtcaatc ttgccatggc agttgggatg gataatggat tgatgacacc tgttgtttac
     7441 aatgctgaga agatgagtct ttcagaactg gttgtagcct ttaaggatgt tattggccgt
     7501 accttggatg gtaaattggc tccaagtgag ttacaaaatt caacattcac aatcagtaat
     7561 ttgggaatgt ttggtgttca gtcctttggt ccgattatta accaacccaa ctcagctatc
     7621 cttggtgtca gttcgacaat cgagaagcca gttgtcgtca atggtgaaat tgtgattcgc
     7681 ccaatcatga gtttaggatt aaccattgac caccgtgtcg tagatggtat ggctggtgct
     7741 aagtttatga aggacttgaa agagttgatt gaaacgccaa tctcaatgtt aatttaagag
     7801 caagagtatt catttttaag atatagataa aggaaggaag acatggcctt agaagtaatt
     7861 atgccaaaag ccggcgtgga tatgacagaa ggacaaatcg tccaatggaa taaaaaagtc
     7921 ggagaatttg taaaagaagg agaaatcctt ttggaaatca tgactgataa agtcagcatg
     7981 gaattggaag ccgaagaaga tgggtacttg attgccattc tcaaaggaga tggtgaaact
     8041 gtccctgtaa cggaagttat cggttacctt ggtgaagaaa gggaaaacat cccaacagct
     8101 ggagcagcat cgccagaagc tagccctgta cctgtagcaa gtacctcaaa cgatgatggt
     8161 aagagcgatg atgcttttga tatcgttgtg attggtggag gtcctgctgg ttatgttgca
     8221 gccattaaag ctgcccaatt cggcggtaag gttgcccttg ttgagaaatc tgaacttggt
     8281 ggaacctgct taaaccgtgg atgtattcca accaagacct accttcataa cgctgaaatt
     8341 attgaaaata tcggtcatgc tgcaaatcgt ggtatcgtga ttgaaaatcc taacttcact
     8401 gttgatatgg aaaaactttt agaaactaaa tctaaagttg ttaatactct tgttggtggt
     8461 gttgcaggac ttcttcgtag ctacggagtt actgttcata aaggaattgg tacaatcact
     8521 aaagacaaga acgtcttggt aaatggttct gaattgcttg aaaccaagaa aatcattctt
     8581 gctggtggtt caaaagtcaa caagatcaac gtccctggta tggaatctcc acttgtcatg
     8641 actagtgatg acattcttga aatgaacgaa gtgccagaaa gccttgttat catcggtggt
     8701 ggagttgtcg gtatcgaact cggtcaggcc ttcatgacat ttggttcaaa agtgactgtt
     8761 atcgaaatga tggaccgtat agttccagct atggatgtgg aagtttctaa gaaccttcgc
     8821 ttgattcttg agcgtaaagg aatgaccatc ttgactggta ctaaactgca agaaatcatt
     8881 gaggaaaatg gtcaacttcg tatcaaggtt gaaggaaaag acgatatcat cgcaagcaaa
     8941 gctcttcttt caattggtcg tatgccagac cttgaaggta ttggagaggt tgagtttgaa
     9001 ttggatcgtg gttgtatcaa ggtcaacgaa tacatggaaa cttcagttcc aggcatttat
     9061 gcaccaggtg acatcaacgg tactaagatg ttggctcacg cagctttccg catgggtgaa
     9121 gtttccgctg aaaatgccct taaaggaaat catgcagttg ccaaattgaa tttgactcct
     9181 gcagccatct acactctccc tgaagtagca gcagtaggtt tgacagaaga acaagcccgt
     9241 gagaaatacg atgttgccat cggtaagttt aactttgctg ctaacggtcg tgctattgca
     9301 tctgacgcag ctcaaggttt cgtaaaagtt atcgctgata agaaatacgg agaaatcctt
     9361 ggtgtacaca ttattggtcc tgcagccgca gaattaataa acgaagcatc aagcatcatc
     9421 gaaatggaaa tcactgttga ggaaatgctg aagaccatcc acgggcaccc aacttactct
     9481 gaagtgatgt acgaagcgtt tgcagatgtt ctaggaatgg ccatccattc acctaagaaa
     9541 aaataaaacg aagatagatt agactggaag gttatttcca gtttctatca tataaagtaa
     9601 agggatatca tgaaatacat tatcaatcat tcaaatgaca ctgcttttaa tattgccttg
     9661 gaagaatacg cctttaaaca ccttttggat gaggatcaaa tcttcctact ttggattaac
     9721 aagccatcta tcattgttgg tcgtcaccag aacactatcg aagaaatcaa ccgtgattat
     9781 gttcgcgaaa atggtattga ggtagttcgc cgtattagtg gtggtggagc tgtttaccac
     9841 gatttaaata acctcaacta cacgatcatc tccaaagaag atgaaaataa ggcatttgac
     9901 ttcaagagct tctcaactcc ggttatcaat accctggctc aacttggggt taaagctgag
     9961 ttcacaggtc gtaatgacct tgagattgat ggtaagaagt tctgtggcaa tgcccaagcc
    10021 tatatcaacg gccgtatcat gcaccacggt tgcttgctct ttgacgttga tttgtcagtc
    10081 ctcgcaaatg cccttaaggt ttcaaaagat aaatttgaat caaaaggtgt gaaatccgtc
    10141 cgtgcccgtg taaccaatat tatcaatgaa ttaccaaaaa aaatcacagt cgaaaaattc
    10201 cgtgatttac tcttggaata catgaaaaaa gagtacccag agatgactga atacgtcttt
    10261 tcagaagaag aattggccga aatcaatcgc atcaaggata ctaagtttgg aacttgggac
    10321 tggaactatg gtaaatcacc tgaatttaac gtccgtcgtg gaatcaaatt cactagtggt
    10381 aaggttgaag tctttgctaa cgttactgaa tcaaaaatcc aagacatcaa gatttatggt
    10441 gacttctttg gtattgaaga cgttgctgca gtagaagatg tccttcgtgg ggtgaaatac
    10501 gaacgcgaag atgttcttaa ggcgctaaaa accattgata tcacacgcta cttcgctggt
    10561 attagccgtg aagaaatcgc tgaagcggta gttggataaa tcaaaaagaa gttggccaac
    10621 ttcttttttt gatatttatt tacataattt aaaatacgta aaatcataaa ctatccagag
    10681 cattcttttg ttcatcacta acaatatggg tatagaggtc agtgacttgt gtgctagcat
    10741 gtcctagttg gtgactgact aaaacttgtg atttagtcgc atcatagagc ctagttgcta
    10801 gtgtatggcg cagtttatgg ggtgttacac gcactttaaa atcctctgag tatttagcaa
    10861 ccattttctc aacgctagaa gcatcgatac gattaggaac acctctgtag agagttaaaa
    10921 aaagggctgt atctgttttt tccgttttat agcgttgatt ccgaatggcc agataattct
    10981 ctaaataagg tttagcaaaa gcagcgacat tgactgagtc acgtttgcaa ccttttcgag
    11041 taacatcaat aaccatcatt tttagattga gatctcttag atctagatta acagcttcag
    11101 ataagcgaac accagatgcc aagagaaggg caataatggc taaatctcgt tctttatttt
    11161 tgttgaatga tgagagagct cgatttgaaa gctgttgtgg gtgctcttga tcgatataag
    11221 ttagaaaacc ttctgtttca tcacctagaa agagtttttg cttgatattt tcagctctgg
    11281 cagcaagggt ttctttcttt ttcttggtgg aaactttttt cattacatta cgatagaaat
    11341 aaggttcccc ctgatcgttt tcaacctcct cggttagata cttgtaaaga ctagaaagtg
    11401 ctgataaggt tcgattgata gttgtctgtg aaacaccttg ttttgttgta ttagcattca
    11461 gcaagggacg ttcacgtaga taaaggataa aggattccat gtctttctta gacatatttt
    11521 ccaaaactga taaaggaata tcggatattt tatcagcgtt tgaaatacca gactccaaaa
    11581 cccagctgaa aaatcggtca tattccttaa ggtattcgta cagggttgta aaactgtagg
    11641 gcacagccag cttagattgg tagtattcca gaacatacca gggcatgagt tgttttagtt
    11701 tgtcgattcg ttccagtaaa atctcacgtt tcatgtattt tctccataag taagtatact
    11761 agtagtatag catagacttg tatatttttc aagaaaatta tagtttttcg gaaagatgtt
    11821 ataggagttt ttcaaagtga taaaaagact tagacctaca aacaaaacag aataaggaaa
    11881 ataataaaaa tctcacccac taaacttctg ttaatactac ataactgaag agctatataa
    11941 ggagctatga gatacaaaac acattgtata atagattgaa actagaatag tacatctgga
    12001 cttctaaaac attgctagaa attgatttga ttgtcctgat tgttttatcc tatttctatt
    12061 tcatttctct atataattgc tattg
//
LOCUS       DAVHEX010000059        11938 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVHEX010000059 DAVHEX010000000
VERSION     DAVHEX010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11938
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.059"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(37..1410)
                     /gene="mnmE"
                     /locus_tag="VVL45_001737"
     CDS             complement(37..1410)
                     /gene="mnmE"
                     /locus_tag="VVL45_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEU4187879.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNTRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTKLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEPLTAQDRQLLEISQETNRII
                     LLNKTDLPETIETSELPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(1547..1729)
                     /locus_tag="VVL45_001738"
     CDS             complement(1547..1729)
                     /locus_tag="VVL45_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEU4187880.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            1853..2452
                     /locus_tag="VVL45_001739"
     CDS             1853..2452
                     /locus_tag="VVL45_001739"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEU4187881.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRDLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPMITSNKEQNYDN"
     gene            2442..2921
                     /locus_tag="VVL45_001740"
     CDS             2442..2921
                     /locus_tag="VVL45_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4187882.1"
                     /translation="MTIELRDVTMENYFDVLNLDVKEYQKQFIATNAISLAEAYVYTK
                     NGDFVAPLAVYDNDAIIGFVMIAYDKKIGISSGNYLLFRFMIDKNFQNQGYFKPIMDK
                     VLDYVRTAPAGLSNKLWLSYEPENEQARFCYLSYGFKETGEISENEVVAIYDLTIEK"
     gene            2931..4010
                     /gene="prfA"
                     /locus_tag="VVL45_001741"
     CDS             2931..4010
                     /gene="prfA"
                     /locus_tag="VVL45_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEU4187883.1"
                     /translation="MNIYDQLQVVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEELAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            4010..4849
                     /gene="prmC"
                     /locus_tag="VVL45_001742"
     CDS             4010..4849
                     /gene="prmC"
                     /locus_tag="VVL45_001742"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEU4187884.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILAENPVKNLMVLDIGTGSGAIALALAKNRPDWSVTAADISQDALDVASENAKNQNLQ
                     IFLKKSDCFTEISEKYDIIVSNPPYISREDESEVGLNVLYSEPHLALFADEDGLAIYR
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKRVRTLKDQFGQDRMVVVDD
                     GQD"
     gene            4833..5435
                     /locus_tag="VVL45_001743"
     CDS             4833..5435
                     /locus_tag="VVL45_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEU4187885.1"
                     /translation="MMDRIRQELENGGAVVLPTETVYGLFAKALDEKAVDHVYQLKRR
                     RRDKALNLNVASLEDILYFSKNQPTYLQKLVEIFLPGPLTIILEANDRVPYWVNSGLA
                     TVGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFNQILEDFDQEVLGLEDDAFL
                     TGQDSTILNLSGDKVKILRQGAIKREDILARLPKISFEEE"
     gene            5437..5868
                     /locus_tag="VVL45_001744"
     CDS             5437..5868
                     /locus_tag="VVL45_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014947335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4187886.1"
                     /translation="MLRDLRETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEEEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIC"
     gene            5930..7186
                     /locus_tag="VVL45_001745"
     CDS             5930..7186
                     /locus_tag="VVL45_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEU4187887.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPKTELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYESLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYED"
     gene            7194..8168
                     /locus_tag="VVL45_001746"
     CDS             7194..8168
                     /locus_tag="VVL45_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEU4187888.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSSKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAKSFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            8168..8782
                     /gene="pmp23"
                     /locus_tag="VVL45_001747"
     CDS             8168..8782
                     /gene="pmp23"
                     /locus_tag="VVL45_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HEU4187889.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     QDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNRTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            8856..9830
                     /locus_tag="VVL45_001748"
     CDS             8856..9830
                     /locus_tag="VVL45_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEU4187890.1"
                     /translation="MRKKLFLTSAAILWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGMEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDIRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            9904..11535
                     /gene="rlmD"
                     /locus_tag="VVL45_001749"
     CDS             9904..11535
                     /gene="rlmD"
                     /locus_tag="VVL45_001749"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU4187891.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTVIDFAELKKDDVIIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSQKNASLNKITNAHYVCDTAENAMKKWLKEGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            11689..11838
                     /locus_tag="VVL45_001750"
                     /pseudo
     CDS             11689..11838
                     /locus_tag="VVL45_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895229.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
BASE COUNT         3724 a         2283 c         2556 g         3375 t
ORIGIN      
        1 aaaatccatg accgaatgaa ggatcatgga tttttcttat tttcctaaac agaattggct
       61 aaagagttgg gtgatgagtt catctggagc agcatctcca gtgatttctc ctagaatttc
      121 ccaagtacgg gtcaagtcaa cttgaagcaa gtcaactggc atccctagtt caagaccttg
      181 gttaacagct tgtaggcttt caacggcctt ctcaatcaag gaaatgtgac gggcgtttga
      241 caagtaggta gcatcttgct caaccaaacc agcattttca aagaagaggt tgttgattct
      301 ctcttcgatt ttatcgatgt tttgattttt aagaactgaa atgcggatga catcttcagg
      361 tagttccgaa gtttcaatcg tttcaggcag gtcagttttg ttaagaagaa taatgcgatt
      421 agtctcctga ctgatttcta ggagttggcg atcttgggcg gttagtggtt cactagcgtt
      481 tagtactagc agaactaggt cagcttcctt aagagctttt ttcgaacgct caactccaat
      541 ttgttcaacg atatcatccg tttcacgaat accggctgta tcaatcaatt tgagaggtac
      601 accattgatg ttgacgtact cttcgatgac atctcgtgtt gtcccagcga tatctgttac
      661 gatagccttg tcctcacgca agaggttgtt gagaaggctt gatttcccaa cgttgggacg
      721 tccaatgata gccgttgaaa ttccttcacg aaggatttta ccacgacgtg ctgtcctaag
      781 gagtttggtt agtaattgct caaactccat tgtcttctct cggacaacag cagtagtggc
      841 ttcctcaaca tcgtcatact caggatagtc gatattgacc tcaacttggg caagtgtatt
      901 gaggatttct tgacgggtat tgttaatgag gtcagaaagg gagccgtcta attgtttgac
      961 cgcaatgttc atggccttgt cagtcttggc acggatgata tccatcacag cctctgcctg
     1021 tgtcaagtct acgcgaccgt ttaaaaaagc acgtttggta aattcaccag gttctgccaa
     1081 ccgagcccct tcacgaatag ctagctggag aatttcattg gtcaccgcaa tcccaccgtg
     1141 ggtgttaatc tcgataatat cctcacgagt gaaggtcttt ggagacttca tagccccaac
     1201 cataacctcg tccatgactt taccagtcag aggatcaata atgtgaccgt agttgagagt
     1261 gtggctggca accttgttca agtcttttcc tttaaaaatc ttttgcgcaa tagcaaaact
     1321 gtctgttccg ctcaggcgga caataccaat agccccttca cctagtggag tagagatagc
     1381 agcgatggta tcaaattcac gtgtaatcat aatgtttcct ttaattaata gctcttttct
     1441 tggaaattct ccaagtttct tttcaaattg taacaaattt aggatatttc ttcaatgaat
     1501 gctacttgga gattgaaaaa gcctaagcaa ttctgcttaa gctagcttat ttagtacgca
     1561 tttccccttg tgggaagtaa gttccttctg gcatgtcgtt gatgatgaca tggacagcag
     1621 attgaggggc tccagtgttg cggacaactg cttccgttac ttccttagca agagctttct
     1681 tttgctcgag cgtgcgtcct tcaaataaat cgatgcgtac aaatggcata atagcttcct
     1741 ccactagttt ttgatttctt ctattttacc acattttgcc gtttaaagct taagaaaatt
     1801 atgatatact agaatgtagc aaaaatttag aaatggacgt gaagcaagaa acatggcaca
     1861 gttgtactat cgttatggga ccatgaactc tggtaaaacg attgagattc tcaaagtggc
     1921 ctataactac gaggagcaag gaaaaggtgt tgtgattatg acctcggctc tggatacgcg
     1981 tgacggcgtt ggctatgtgt cgagtcgaat tggcatgaaa cgccctgccc ttgcgattga
     2041 ggaaacaacg gatatctttg gctatatccg agacctacca gaaaaacctt actgtgtttt
     2101 ggtcgatgaa gcccagtttc tcaaacgcca ccatgtttac gacctagctc gtgttgtgga
     2161 cgagttagac atacccgtca tggcttttgg cttgaaaaat gactttcgca atgaattgtt
     2221 cgaaggttcc aaatatctct tgcttttagc agacaagatt gacgaaatca agaccatctg
     2281 tcagtattgc aagaaaaagg cgaccatggt gttgcgaaca caggatggac tgcccgttta
     2341 cgatggagaa cagatccaga ttggtggtaa cgaaacctat atctcagttt gccgtaaaca
     2401 ttattttgcg ccaatgataa cctctaacaa ggagcaaaac tatgacaatt gaactaagag
     2461 atgttacaat ggaaaattat tttgatgttt taaatttgga tgtcaaggaa tatcaaaaac
     2521 aattcattgc aaccaacgca attagtttag ctgaagcata cgtctacact aaaaatggag
     2581 attttgtagc tccattggca gtttatgata atgatgcaat tataggtttt gtgatgatag
     2641 cttatgataa aaagatcgga attagtagtg gaaattattt actatttcgt tttatgattg
     2701 ataagaattt tcaaaatcaa ggatatttta aaccaattat ggataaagta ctagactatg
     2761 ttcgaacagc gccagcaggt ttaagcaata aactttggtt gtcttatgaa ccagaaaatg
     2821 aacaggcaag attttgttac ctcagttatg gatttaaaga aactggggaa atatccgaga
     2881 atgaagtagt agcaatctat gatttaacaa ttgagaaata aggagaaaaa atgaacatct
     2941 atgatcaact acaagttgta gaagaccgtt atgaagaatt aggagaattg ctgagtgacc
     3001 ctgatgtcgt ttcagacacc aagcgtttta tggagctttc aaaagaagaa gcttccaatc
     3061 gtgacaccgt aatagcctac cgtgagtata aacaagtcct tcaaaatatc gtcgatgccg
     3121 aagagatgat taaggaatca ggcggagatg cggacttgga agaattggcc aagcaagaac
     3181 tcaaagatgc caaggctgaa aaagaagaat atgaagaaaa actgaaaatt ttgctccttc
     3241 caaaggatcc aaacgatgac aagaatatca tccttgaaat ccgtggagca gctggtggag
     3301 acgaagcggc acttttcgct ggagatttgc taactatgta ccaaaagtat gcggaagccc
     3361 aaggttggcg ctttgaagtc atggaagcct ctatgaatgg tgtcggtggt tttaaagaag
     3421 tggttgctat ggtttcaggt cagtctgtat actctaagct taagtatgaa tcaggtgccc
     3481 accgtgtgca acgtgttcct gtgacagaaa gccaaggccg tgttcatact tcgacagcga
     3541 cagttcttgt tatgccagaa gttgaagagg ttgaatacga cattgatcca aaagaccttc
     3601 gtgtcgacat ctatcacgcc tctggtgctg gtggacagaa cgtcaataag gttgcgactg
     3661 ccgttcgtat cgttcacttg ccaaccaata tcaaggttga gatgcaggaa gaacgtaccc
     3721 agcagaagaa ccgtgagaag gccatgaaaa tcatccgtgc ccgtgttgct gaccactttg
     3781 ctcagattgc tcaagatgag caggacgctg agcgtaagtc gacaatcggt actggtgacc
     3841 gttcagaacg gatccgaact tataacttcc cacaaaaccg tgtcacagac caccgtatcg
     3901 gcttgaccct ccaaaaacta gatacgattt tgtctggtaa attggacgaa gttgtggatg
     3961 ccttggtgct ttatgaccaa acacaaaaac tagaagaatt aaacaaataa tgaaattagc
     4021 tcaattattt tcaaattttg aagaagagtt gataagacaa ggagaggaag cggaaagcct
     4081 ctcttttgtc tatcgtagtc tgaaaaatct atcttttaca gactttattt ttgccctcca
     4141 gcaggaggtg acaacagagg aagaaaaaca atttgtagaa gatatttacc agcaactagc
     4201 agctcacaag ccagctcagt acatcattgg acaggcagat ttttatggaa tgcatttaaa
     4261 ggtggatgaa cgggttttga ttcctcgtcc agaaacagag gagttggtgg agcttatcct
     4321 ggctgaaaat cctgtgaaaa atcttatggt cctagatatc ggaacaggta gtggagctat
     4381 tgctctcgct ctagcaaaaa acagaccaga ttggtcagtg acggcagcag atatttccca
     4441 agatgcttta gatgtagcta gcgaaaatgc taaaaatcaa aatcttcaaa tatttttaaa
     4501 aaaatctgac tgttttacag aaatttctga aaaatatgat ataattgttt ccaatccacc
     4561 ctatatctct cgtgaagatg agtcagaggt aggcttgaat gtcttgtatt cggagcctca
     4621 tctagctctc tttgcagatg aggatggcct agctatttac cgtagaattg cggaagatgc
     4681 aaaagactat ctcaaagatg gtggtaagat ttaccttgaa attggataca agcaaggtca
     4741 aagtgttcct gaacttttta ggaaacatct tcctgaaaag cgagtacgaa cactcaagga
     4801 ccaatttggt caagatagga tggttgtcgt tgatgatgga caggattaga caagagttgg
     4861 aaaatggtgg agctgtcgtt ttgcctacag agacagttta cggtctcttt gctaaggcgc
     4921 tagacgaaaa agcagttgac catgtttacc agcttaaacg tcgacgtaga gacaaggcgc
     4981 tcaatctcaa tgttgcctct ttagaggaca tcttgtactt ttcaaagaat cagccaactt
     5041 atcttcaaaa acttgtagag atctttttac caggtccctt gaccattatt ctcgaagcta
     5101 atgaccgagt tccctattgg gtcaattctg gccttgcaac tgttggattt cggatgccta
     5161 gtcatcccat tacccttgat ttgattcgag agacaggtcc cttgattggg ccgtctgcca
     5221 atatctcagg tcaggcgagt ggagtgacct ttaatcaaat tctagaggat tttgaccaag
     5281 aggttctggg tctggaagac gatgcttttc taactggaca ggattcaact attttgaatt
     5341 tgtcgggaga caaggtgaaa atcttacgtc aaggggccat taagcgagaa gatattcttg
     5401 ctcggttgcc aaagatttct tttgaggagg aatgagatgc taagagattt aagagaaact
     5461 gatgtgaaag ctatatgtga catcaaccaa gaggctttgg gttatacttt tagtccagag
     5521 gaaacggcta gccaactagc tagactgtct caggattccc atcatttcct acttggctat
     5581 gaggatgcag ctaatcatgt cttacttgga tatgtccacg ctgaagttta cgaatcactc
     5641 tattccaaag caggatttaa tatcttagct ttagcagttt cacctcaagc gcaaggtcaa
     5701 ggtattggta aaagtttact acaagggttg gaagaagaag caaaaagatg tggttatggg
     5761 tttatccgct taaattctgc caatcatcgt ctgggtgctc atgcatttta tgaaaaagtt
     5821 ggctatactt gtgataaaat gcagaaacgg tttattcgca tctgttagtt tgattttctt
     5881 attgtaaaat caaactaatg gactagtcac acaataaagg agaagaccta tgatttttga
     5941 caaagatgat tttaaagcat atgatgctga tctctggaat gctattgcca aagaagaaga
     6001 acgccaacaa aataatatcg agttaattgc ttcggaaaac gtagtttcca aggctgttat
     6061 ggcagctcaa gggtctatct tgacaaataa atatgccgag ggttacccag gacgccgtta
     6121 ttatggtgga actgatgtag tagacgttgt agagactctt gctattgaac gcgcaaaaga
     6181 aattttcggt gctaaatttg ccaatgttca accacattca ggaagccaag ctaactgtgc
     6241 ggcttacatg tccttgattg agccaggtga tacggttatg ggaatggatt tggcatcagg
     6301 tggtcatttg actcatgggg ctcctgttag cttctctggt caaacctaca actttgtttc
     6361 ttatagtgtt gatcctaaaa cggaactctt agactttgat gctatcttga aacaagccca
     6421 agaagtaaaa ccaaaactga ttgtagctgg tgcttcagcc tattctcaaa ttatcgattt
     6481 ttcaaaattc cgtgaaatcg cagatgctgt cggtgcgaag ctcatggtgg acatggccca
     6541 tatcgctggc ttggttgcgg ctggccttca tccaagccca gttccatacg ctcatatcac
     6601 aacaacaacg acccacaaaa cccttcgtgg acctcgtggt ggtttgattt tgaccaatga
     6661 cgaagaactt gctaaaaaaa tcaattcagc tattttccca ggtattcagg gcggtccttt
     6721 agagcatgtt gtggcggcta aggcagtttc cttcaaagaa gttttggatc cagccttcaa
     6781 ggaatatgct gccaatgtaa ttaagaacag caaggctatg gcagatgtct tcttgcaaga
     6841 ccctgatttc cgtattattt caggtggaac tgaaaaccat ctcttccttg ttgatgtgac
     6901 taaagttgta gaaaacggca aagttgctca aaacttgttg gatgaagtca atattacctt
     6961 aaataaaaac tcaatccctt acgaaagctt gtcaccattc aagacaagtg ggattcgtat
     7021 cggagcagca gccattactg cacgtggatt tggtgaagaa gaaagtcgca aagtggctga
     7081 actcatcatt aaaaccctta agaattcaga aaatgaggct gtattagaag aagtgagaag
     7141 tgcagtcaaa gaattgacag atgccttccc attatacgag gactaaaact tttatggaca
     7201 tttatattaa gaaagccatt attcaccagt tcagtccgga tgataccgaa ctgttcttag
     7261 cagataagtt tctcaatatt actccaaaaa tcgaagaata cctgcgtaaa aaaattgaac
     7321 atgtgtattc agatgaagcc aagactggga ttttcgaaga agaaaatccc ttcttcaatc
     7381 atattacaga cgatttgttg gagacatcag taacgctggc taatctctgg aaagaggagt
     7441 ttagcatttc tgaaaatctc aagaccaatg acttgatttt tgtgcaattt tctaaagaag
     7501 gtgtagaaca tttcgctttc ttgcgaattg ccctgcggga gaccttgacc cacctcggag
     7561 gagaagttga taatccaatc aagctaactc agaataacct gcctggattt ggaacaggag
     7621 ctgatgaggc cttggtggtc aatcttcaga gtagcaagta tcacctgatt gaaaaacgaa
     7681 tcaagtacaa cgggactttt ttgaactatt tttcagataa tcttcttgct gtcgctccta
     7741 agatttctcc taaaaaatct atcaaggaac tggaaaaaac agcccagaga attgctaaat
     7801 cttttaacac agatgatttt caatttcaat ccaaggtcaa atcagctatt ttcaacaacc
     7861 tagaagaaag caatgaattg tcacctgaga aattggctaa tgaccttttt gacaacaatc
     7921 tgacggctcg tttgagcttt attgaccaag tcaaagaagc cgtaccagaa cctgttcaat
     7981 ttgatgaaat tgatgccagt cgccaattaa agaaatttga aaaccaaaaa ctctccttat
     8041 caaatggaat tgagctcatc gttcccaata acgtctatca agacgccgag tctgttgagt
     8101 ttatccaaaa cgaaaatgga acctactcta tcttaatcaa aaatatcgag gatatccaaa
     8161 gtaaataatg tttaaacgaa ttcgaagagt gcttgtacta gcagtcttcc tttttgctgg
     8221 ctataaagct taccgcgttc atcaagatgt caaacaagtc atgacctatc aacccatggt
     8281 gcgagaaata ttgagtgaac aagacacccc agcaaacgaa gagcttgtgc ttgctatgat
     8341 ttatactgaa acaaaaggaa aagaaggcga tgttatgcag tctagtgagt ctgcaagtgg
     8401 ttccaccaac accatcaatg ataatgcctc tagcattcgg caaggcattc aaactctgac
     8461 aggcaatctc tatctggcgc agaagaaggg ggtagatatc tggacagctg ttcaagccta
     8521 taattttgga cctgcctata tcgattttat cgcccaaaat ggcaaggaaa ataccctggc
     8581 tctagccaaa cagtactctc gtgagactgt tgcccccttg cttggtaata ggactggaaa
     8641 gacttatagt tatattcacc ccatttccat ttttcacggt gctgaactct atgtaaatgg
     8701 aggaaactat tattattcta gacaggtacg acttaacctt tacatcatca aatgtttcac
     8761 tctcttttca acatctggct agaccaggtg tttttgttat aagttttctt taagatagat
     8821 atattactct aaagaagtaa aggaggaaat tccctatgag aaagaaactc tttctgacta
     8881 gcgctgcgat cttgtgggca gtaacagcta tgaatagcgt ccatgcagca acagatgttc
     8941 aaaaagttat cgatgaaacc tatgtccaac ctgaatatgt cctaggttcc tccctatctg
     9001 aagaccaaaa aaatcaaact cttaaaaaac tgggctacaa tgcctcaaca gataccaaag
     9061 aactcaagac catgacacct gatgtttatt ctaaaatcat gaatgtggcc aatgactcta
     9121 gcttacagtt gtattcatca gccaagattc aaaagctagg tgacaaatcg ccacttgagg
     9181 tcaagattga aacaccagaa aatatcacta aggtgactca ggatatgtac cgaaacgcag
     9241 cagtaacgct gggtatggaa catgccaaaa tcactgtagc agcccctatt ccagttacag
     9301 gagaaagtgc tttagcaggg atttactatt cgctagaggc taatggagcc aaggtgccac
     9361 aagctaataa agatttggct caagaagagc taaaagcttt gtcagatatc aatgctgaaa
     9421 acaaggacaa atcaggctat gatgctaata aattaaacgt tgccctagct gatatcaagt
     9481 caggactcgc caaagctaaa gaaagtaagg gaaatctgac agaagaagat atccgcaaga
     9541 ttgttgaaga taccttaaaa aattacaaac ttgatcaggt cataacagga aaccagatca
     9601 atatcatcat caattttgcc ttgaatctct caaagagtga tatcctcagc aatgcagatt
     9661 tcactaaaac cctaaatgac cttaaacaaa gcatcgtatc acaagctggc gacagtttta
     9721 aaaatatcaa ccttaacttt gatgcggata aggcgctaga ggacggtggt aacttcttaa
     9781 gctccctctg gcaagccctt gtcaacttct tcaagagttt tggttcttaa gaaatttcat
     9841 ggtataatgg atggtaaccg taacgttgcc gtcttttttg tcggccaata gaaagagaaa
     9901 agaatgttaa agaaaaatga tattgtagaa gttgaaattg ttgatttgac ccatgaaggg
     9961 gcaggagttg ccaaggtaga tggtttggtc ttttttgtag agaatgcttt accgagtgaa
    10021 aaaattctca tgcgtgtcct caaggtcaat aaaaagattg gctttggaaa agttgaaaaa
    10081 taccttgtcc agtcaccaca ccgtaatcaa gatctagatt tggcttacct gcgttcagga
    10141 atcgcggatt taggacacct ttcttatcca gaacagctca agtttaaaac caagcaagtc
    10201 aaggacagtc tctacaagat tgctggaatt gcagatgtag aagttgctga aacgcttggt
    10261 atggaacatc cagtcaagta tcgcaataag gcgcaggtgc ccgttcgtcg agtgaatggt
    10321 gtcttggaaa caggattttt ccgtaagaat tcgcataacc tcatgcccct tgaagatttc
    10381 tttatccagg atcctgtcat tgaccaagtc gtagtagctc ttcgagacct gctccgtcgt
    10441 tttgatttaa aaccttatga cgaaaaggaa cagtctggat tgattcggaa tcttgtggtg
    10501 cgtcgtggtc actattcagg acaaatcatg gtcgttttgg tgacaactcg tccaaaagtt
    10561 tttcgtgttg accaattgat tgaacaagtt atcaagcagt tcccagagat tgtgtctgtc
    10621 atgcaaaata tcaacgacca gaataccaat gcgatttttg gtaaggagtg gcgcactctt
    10681 tatggtcaag actatattac ggaccagatg ttgggaaatg acttccaaat cgctggccca
    10741 gccttttacc aagtcaatac tgaaatggcg gagaaactct atcaaacagt cattgacttt
    10801 gcagagttaa aaaaagatga tgtgattatt gatgcctatt ctggtattgg aaccattggt
    10861 ttatcagtcg ccaagcatgt caaagaagtc tacggtgttg aactgattcc agaagcagta
    10921 gagaatagcc agaagaatgc ttctttgaac aagattacta atgcccacta tgtctgtgac
    10981 acggctgaaa atgccatgaa gaaatggctc aaggaaggta ttcaaccaac cgttatcttg
    11041 gttgatcctc cacgcaaggg cttgacagaa agctttatca aagcaagcgc ccaaacagga
    11101 gccgatcgca tcgcctatat ctcctgcaat gtcgcaacca tggcgcgtga tattaaacta
    11161 taccaagagt tgggatatga attgaagaaa gtccagccgg tggatctatt tcctcaaacg
    11221 catcacgtcg agacggtagc acttttgtcc aaactcgatg tcgataagca cataagtgtt
    11281 gaaattgagc tggatgagat ggatttgaca agtgcggaga gcaaagcaac atatgctcaa
    11341 atcaaagaat atgtttggaa taaatttgaa ttaaaagttt cgacattata tattgcacag
    11401 ataaaaaaga aatgtggaat agaattacga gaacattaca acaagtctaa aaaggataaa
    11461 caaattattc cacagtgtac acctgaaaaa gaagaagcca tcatggatgc tttgagacac
    11521 ttcaaaatga tttaatagaa aagaatgaca gtatatgact ttctgcattt attacattcc
    11581 tacttggtat aggaacagct attattcctt tcttgcaagg tatcaattag aaaataggct
    11641 caatataaag attgatagga tcatttttat atttaaagga gcgttgaaat gattgataaa
    11701 ggcaacaaaa aattttagga taaatttgct aagttgtatg cctcttttat gaaaaaagat
    11761 aaagaggttt atgataaagt ttgtgaatat cttagtcctc atttgaataa agatatggag
    11821 gtgcttgaac ttgcttgttg gtttcgtgtc ataacagtta tagaggcaaa tagttatgta
    11881 aatataagga gttcaagact tctaccaaag tttaaaactc aaaaaataaa tagttggt
//
LOCUS       DAVHEX010000060        11799 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVHEX010000060 DAVHEX010000000
VERSION     DAVHEX010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11799
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.060"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(394..1590)
                     /gene="tuf"
                     /locus_tag="VVL45_001751"
     CDS             complement(394..1590)
                     /gene="tuf"
                     /locus_tag="VVL45_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU4187892.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(2512..3381)
                     /locus_tag="VVL45_001752"
     CDS             complement(2512..3381)
                     /locus_tag="VVL45_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEU4187893.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTENPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKATEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
     gene            complement(3605..4213)
                     /locus_tag="VVL45_001753"
     CDS             complement(3605..4213)
                     /locus_tag="VVL45_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU4187894.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVISGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPEKPIPQPNPEHPSVPTPNPELPNQETPTPDKPTPEPGTPKTETPVNPDPEVPTYE
                     TGKREELPNTGTEANATLASAGIMTLLAGLGLGFFKKKEDEK"
     gene            complement(4171..5277)
                     /locus_tag="VVL45_001754"
     CDS             complement(4171..5277)
                     /locus_tag="VVL45_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEU4187895.1"
                     /translation="MNIILIAKLLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANY
                     YKEGRQEKLVESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPA
                     NAYQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
     gene            complement(5388..5642)
                     /locus_tag="VVL45_001755"
     CDS             complement(5388..5642)
                     /locus_tag="VVL45_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187896.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRITNRLANDLNNVLKQV
                     DKDTPNTPTWLNSAASKAKDDDRVYKLLKTLIPGENYLSC"
     gene            complement(6000..6446)
                     /locus_tag="VVL45_001756"
     CDS             complement(6000..6446)
                     /locus_tag="VVL45_001756"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU4187897.1"
                     /translation="MDETGIDRYLYRPYAGAPRGEKVYEKISGRRFERTSIVAGQVDG
                     EFIAPMIYKKSMTSDFFVEWFKTQLLPALKTPHVIVMGNAGFHPKNILDELCIQDKHF
                     FLPLPPYSPDLNPIEQAWAILKKKVTDVLREVPTIFECLECFFKTR"
     gene            complement(6519..6844)
                     /locus_tag="VVL45_001757"
                     /pseudo
     CDS             complement(6519..6844)
                     /locus_tag="VVL45_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331855.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            7078..8796
                     /locus_tag="VVL45_001758"
     CDS             7078..8796
                     /locus_tag="VVL45_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEU4187898.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDIEYLKEVKDINI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKIANIEAE
                     INAFVK"
     gene            8907..9254
                     /locus_tag="VVL45_001759"
     CDS             8907..9254
                     /locus_tag="VVL45_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU4187899.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFVHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(9533..10369)
                     /locus_tag="VVL45_001760"
     CDS             complement(9533..10369)
                     /locus_tag="VVL45_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU4187900.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPEILKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTNLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(10382..11011)
                     /locus_tag="VVL45_001761"
     CDS             complement(10382..11011)
                     /locus_tag="VVL45_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU4187901.1"
                     /translation="MLELRNINKVFGDKQILSNFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(11021..11662)
                     /locus_tag="VVL45_001762"
     CDS             complement(11021..11662)
                     /locus_tag="VVL45_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU4187902.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
BASE COUNT         3454 a         2461 c         2149 g         3735 t
ORIGIN      
        1 ttgatttcat attactattg tataatgctt tttgatttta gtctagtatt atactcttcg
       61 aaaatctctt caaaccgcgt cagcttcgcc ttgtcgtata gatgttactg acttcgtcag
      121 ttctatctac aacctaaaaa cagtgttttg agcagtctgt agctaacttt ctatagtaaa
      181 atgaaataag aacaagacaa atcgatcagg acagtcaaat cgatttctaa caatgtttta
      241 gaagtagatg tgtactattc tagtttcaat atattatagt ttgctctttg attttcattg
      301 agtattagtt caatatctaa aaaaagaacc ttgccaagca agattctttt agtgtctaac
      361 ttaaataatt gttcttctgg gaactaaatc gaattaagct tcgatttctg taaccatacc
      421 tgaaccaaca gtacgtccac cctcacggat agagaatgta gtaccttgtt ctacggcgat
      481 tgggtgaatc aactcaacgt cgattgtcac gttatcacca ggcattacca tttcagtacc
      541 tgctggaagt tcgattgaac ctgtaacgtc agtagtacgg aagtagaatt gtggacggta
      601 gttgttgaag aatggagtgt gacgtccacc ttcttcttta gtaaggatgt agacttcacc
      661 tttgaattta gtgtgtgggt tgattgaacc tggtttagcg ataacttgtc cacgttcgat
      721 ttcatcacgt tgaacaccac gaagaaggac acctacgtta tctccagcaa gaccttcgtc
      781 aagttgttta cggaacattt caacaccagt aacaactgct ttttgagttt cttctttgat
      841 accaacgatt tcgatttcgt cgttgacttt aacgatacca cggtcgatac gtcctgaagc
      901 aactgtacca cgtccagtga ttgagaatac gtcctcgact ggaagaagca atggtttgtc
      961 agtgtcacgt tctggttctg ggatatactc atcaactgtg ttcatcaatt ccataacgat
     1021 gtcttcgtat ttagagtcac cttcaagagc tttaagtgct gaaccttgga taactgggag
     1081 atcgtcacct gggaagtcgt attctgacaa taggtcacgg atttccattt caaccaattc
     1141 aagcaattct tcgtcgtcaa ccaagtcaac tttgttcatg aagacgataa ggtgtttaac
     1201 accaacctga cgtgaaagaa ggatgtgctc acgagtttgt ggcattggtc cgtcagttga
     1261 agctactaca aggatagctc cgtccatttg agcagcacca gtgatcatgt ttttaacgta
     1321 gtccgcgtgt cctggagcgt cgatgtgagc gtagtgacgt ttttcagttt cgtactcaac
     1381 gtgcgcagtg ttgatagtga taccgcgttc gcgttcttct ggagcagcat cgatagacgc
     1441 atagtcttta ggttggttaa ctgatgaagg caagcgacgt gccaaaacag ttgtgatagc
     1501 tgcagttagg gtagttttac cgtggtcaac gtgtccgata gtaccaatgt taacgtgtgg
     1561 tttactacga tcgtattttt cttttgccat ttgagtaaaa gcctccaata aaatatattt
     1621 tatagataga cagtaggcaa tacagtctaa ctttccttac tattttatca aatttcagct
     1681 aaattgcaag tgttttacaa cgtttttgtg aattattctt ggtaaaaatt taactaatta
     1741 atagaaagta ctaaatagac ttatccttca aggagttttc agcattatcc atccactcac
     1801 ttgctgaaaa tgtaaacagt ctctggttta tatagtaaaa tgaaacaaga atagggcaaa
     1861 tcgttcagga cagtcaaatc gatttctaac aatgttttag aagtagatgt gtactattct
     1921 agtttcaatc tactatacaa cctcaaaact gtgttttgaa caacctgcgg ctagcttctt
     1981 agtttgccct ttgattttca ttgagtataa aattgcagat aaaacgataa caatcatagg
     2041 actcagcaga gtcaatgtag gtcgaagaat agccccccta atcgcttatt ttatagtaaa
     2101 atgaaatata aactgaacat tttgttgagg aaggtaaatc aattcctcac aatgtttgag
     2161 aaagaaggga ggactattcc aacttcaacc cactataaag cagacttaca agatagggaa
     2221 cccatctccg ataatattcc gataatatat agtagaatga aaactgcaca atttgattgg
     2281 agaattcaaa ccaatttctc acaatgtttt agaaacaagg gtctattatt tcaactgcaa
     2341 cctactatag tccattgaag ctggaatagc ccactgagac ttctaaacca ctgctggaga
     2401 ggagtttgtt tagagtttct ttcatttccc taaaaaatca aaaagagaga accagtctga
     2461 tcctctctta cttatagatg gtgctcactc aaatgttaaa ggagctattt cttagagata
     2521 aaggaatttg aatacagcta ctgccgcaat tgctgctgcg ataggtgcta ctactggtac
     2581 ccaagaatac caccattttg aatcgccttt atgctcacca agaactgatt tgggaaggaa
     2641 agcatgaagg agacgtggtc ccaagtcacg ggctgggttc aaggcaggtc ctgtaggtcc
     2701 tccaagtgat gttaccaaag ccataacgag gaatccaagt gccaagtgag ccacagaaag
     2761 tcctgaagca gtgtgtggag ccacctgtgc tttaatagcc aagtcagaaa aatcaactgt
     2821 ttgtcctgct tctgttgcct tttgtttcat gaattgaagc acttcagcac caaagaagtt
     2881 tttagtcaaa ccaagagctg caaagaacaa aacaaatgaa ccaacaaact cattaatcaa
     2941 accattgaca gttgctgcat agcgactttc ttttgtacca tggtcaatac ttgaaatagt
     3001 tgagaaagtt cccaagatgt tatttgggtt ttcagttttc aagtagaatg gacggtatgt
     3061 tgccacaact aaggcttggc caaagatagc ccccaagact tgcgcgataa tgtaaggtac
     3121 cacttgtgcc caagggaaaa gaccgctaac tgcaagccct agagtgaaag cagggttgat
     3181 gtgattccca gatacgttac caaacatcaa ggctgggatc ataaccccca taccataacc
     3241 aacagcaatg acgatccagc cactttggtg acctttcgta cctttaagtt caacgttggc
     3301 aactgcacca ttcccaagaa tgatcaaaat ggcagttccc aaaaattcag tggcatactt
     3361 cagtgcccat gtgaaatcca tttgctagat tctccttaaa attttttaga tattccttat
     3421 tttatcaatt tttaccctat ttagcaactg atttctggaa atatttttaa aaaaatagga
     3481 ttcatagttt cccagttcaa cagacttgta agcgttttta ttctttacga aagacctcgt
     3541 tctcaaaacc aggaaaaaag cccacatctg tgagcttttc ctggttccta gattctaaaa
     3601 tctattattt ttcatcttct tttttcttga aaaatcctaa tcctagacca gctaacaagg
     3661 tcatgatacc agcactagcc aaggtagcat tagcttctgt acctgtgttt ggcaattcct
     3721 ctctcttacc tgtctcataa gtcggaactt ctgggtctgg attcactgga gtttcagttt
     3781 ttggagtacc tggttctgga gttggtttat ctggtgttgg agtctcttga tttggtagtt
     3841 ctgggtttgg tgtcggaaca cttggatgct ctggatttgg ttgtgggatt ggtttttctg
     3901 gtgttgataa acggtcatac cttaccgtta tttctttatc actagagtct gacgtaactt
     3961 cttgtgattc aactgttgga atatctggat ctttgtactt gtcaatctta ccagatataa
     4021 cctcgtccca gtttcctgtt gtccattcac cgtaggttac aactcccgtg accttgttct
     4081 cagtttttgt acggcttaag gttacaggtt gaacaacatc ttcttttaca ttttggttcg
     4141 taactttatc aacgtaatga atgatacgcg ttatagtctt cgtctcagta gaggttgctg
     4201 ttttgggaac cactgtttcc tcaacattct cacggtagta atagtcaact gttgcaccgt
     4261 cttctggtac gcatttgcag gagttgctac caaggtgtat gttgttttcc ttgtgataac
     4321 tcggtcttct ttgtcctcag ttgttgtttt cccttcaata gtttttgatt ctgtggtata
     4381 ctcagaacct atcgctaaat cagcttttat aacagactct accaacttct cttgcctacc
     4441 ttctttatag taattggctg ttactgtagc agtggttggc gcttcgcttt ctctataaac
     4501 taaggtcact gttctacctt cgcttacaat attcccagtt aaacttgcag aatttgtatc
     4561 tgcttcttta aaagtataat attttccgtc agtagtagtc atgctactga gttttttatc
     4621 tgtgacatta tagctggtac caatcagttg ttttttattg gtaatgtagg ttccgtcact
     4681 ttctttttct ccaattccag tatcattttt caatgatagc aaacgccctt gttcatcaac
     4741 atagcgaact ttcacatttt ctgagattag ttctgccaat tctgaggttt ttttcttttt
     4801 cttgatttct tcggttattt tccctttctc ttcttcggga atatttagtt ttggaatgat
     4861 tttttcaaca acggttcgtg atggttccac agtatcttgg atgactgaaa agtcagctag
     4921 aattgggaga ttatactgaa cacggtgact ttgagtgttt actcctactc tttcattatt
     4981 ctctgaaaat acttgtacgg tataagaaac aacatctttt cctaatagaa catccccagt
     5041 agagaaatag ccgccttttc ctagtttgct atctccagag cccacttctt tcctaatctt
     5101 atcagatagt tttttaccag tcagtacatt cgttcgcaca atccctttgt ctacccctac
     5161 aaagtgggag aactttttga actcttcaga accagatcta gcccaaccat tattaagggc
     5221 atttgctttt gtatttgtat tctctctcaa aagtttggcg attagaatta tattcatcgg
     5281 cacttagagt tgctgctata tctgactctt gaataccaac ttccttacta ccatttctag
     5341 cggcagtata tgtgtattta tctgtttcta cttctagctg attatcctta acatgatagg
     5401 taattttctc ctggtataag agtcttcagt agtttatata ctctgtcatc atctttagct
     5461 ttagaagcag ctgagtttag ccaagttggg gtattaggag tatctttatc aacctgtttc
     5521 aagacattat tcaagtcatt tgctagccta ttggttatcc tcactgtgaa agttttcttc
     5581 cagtctgaac cacttttatt agacttagat atgctcatga aatcctggtc aacattcaac
     5641 acaatatcgc cttgtttttt agcatcttcc gttgcttctg cttcaaattg atcatggagt
     5701 ttttgttgct ctggtgtata gtttttttat ttacacgatt taatgtacta ttttttattt
     5761 tagtattaag aactccatta atacgatttt taaactctgc ttcaaaaaat cccttaattt
     5821 gttggtgcgc aagttcaaca ttcaaacctt tcttatacaa gtcttcattc tgatacaagt
     5881 cttcattctg aacgatttcg gctacacggt gaatggtttg attgtatacc gtcttctctt
     5941 aaccagcagt atccttagtt ttaatgacta ctcgataggt tcctttagaa ccgttatagt
     6001 catctagttt taaaaaagca ttccaaacat tcaaaaatag ttggaacttc ccttaataca
     6061 tccgtcactt tctttttcaa gatagcccaa gcttgctcaa taggattcaa atccggtgaa
     6121 taaggtggta gaggtaagaa aaagtgttta tcttggatgc agagttcatc caaaatgttc
     6181 ttgggatgaa aaccagcatt gcccatgaca ataacatgag gtgtcttcaa agcaggtagg
     6241 agttgcgttt tgaaccactc cacaaagaaa tcgcttgtca tgcttttctt gtaaatcatg
     6301 ggagctataa actctccgtc tacttgtcct gcaacaattg aagttcgctc aaaacgacgt
     6361 ccgctaatct tttcatagac tttctcccct ctaggagccc ctgcataagg acgatagaga
     6421 tagcggtcga ttcctgtttc atcaatataa acaggtgcta ggtgctttaa actattaaaa
     6481 ttcttaagaa ataaggctac ttttctgggt cttgttcctt aaagctcgtc atcttttttt
     6541 aaagtgacct taatctgctt taaagctgcc catactgaag gaacagcaca atcaaaatgt
     6601 gccgcaattt cccgtaaaaa agcatctgga tgagcctcta caaaggcttt caattcctct
     6661 aaaggaatct ttcggttttt gacgactcgc tttttcctct ctatgtgttc ttgttcacgt
     6721 ctttcttttc ccacgtgaag agagttctga cgccaacacc aaaaaacttg gctgcctcga
     6781 catggctgtg cccctctttg atgtaatcta atgctcgctg tttaaaatca gtactatatg
     6841 ccatagcttt attataatat gcttgtttaa aactaagtaa ctatatcaat gttttgttat
     6901 gagaagactt acttttttta ctgaaattga atttttccta gcctcaacat catataaccc
     6961 caaaattcaa tctgacctgg tttgaagaga tgttcgaagc agataaggtt ttactaaaac
     7021 taaaaaaaat gatataatta aactgattaa atgttttaaa aataaaggag aatttgaatg
     7081 tcttaccaag aaaattacca gaaatgggtt gattttgtgg agcttcctga ctaccttcgt
     7141 caagatttgg aaaatatgga cgaaaaaact aaggaagatg ccttctatac aaatcttgaa
     7201 tttggtactg caggtatgcg tggcttggtt ggtgctggta caaaccgtat caacatctac
     7261 gttgttcgcc aagctactga aggattggct cgtttgattg agtcaaaagg tggaaacgag
     7321 aaagaacgcg gtgtagcaat tgcctacgat agccgtcact tctcacctga gtttgccttt
     7381 gaatctgcgg cagttcttgc taaacacggc atcaaatctt acgtatttga aagccttcgt
     7441 ccgactccag aactatcatt tgcagttcgt catctcaact gtttcgcagg tatcatggtc
     7501 acagccagcc acaaccctgc tccatttaac ggttacaagg tttacggtga agacggtgga
     7561 caaatgcctc cacacgatgc ggacgctttg actacttata tccgtgcaat cgaaaaccca
     7621 tttgcagttg aagttgctga tgtggaaact gaaaaagctt ctggcttgat tgaagttatc
     7681 ggcgaagctg ttgacataga ataccttaaa gaggttaagg acataaacat caacccagcc
     7741 ttgattgaag aatttggtaa agacatgaag attgtctaca caccacttca tggtactggt
     7801 gaaatgttgg ctcgtcgtgc tcttgcccaa gcaggatttg actctgttca agttgttgaa
     7861 gcgcaagcaa ctgctgaccc agacttttca actgtaacat ctccaaaccc agaaagccaa
     7921 gcagcctttg cccttgctga agaacttggt cgtcaagttg gtgcagatgt tcttgtcgca
     7981 actgacccag acgctgaccg tgttggtgtt gaagttcttc aaaaagatgg tagctacctc
     8041 aacctttcag gtaaccaaat cggtgctatc atggctaaat acatcttgga agctcacaaa
     8101 aacgctggaa ctcttcctga aaatgccgct ctctgcaaat ctatcgtttc aactgacttg
     8161 gtaacgaaga ttgctgaaag ctacggcgca actatgttca acgtcttgac aggtttcaaa
     8221 tttatcgctg aaaaaatcca agaattcgaa gaaaaacaca accacactta catgatggga
     8281 tttgaagaaa gcttcggtta cttgattaaa ccatttgtac gtgataaaga tgccatccaa
     8341 gccgttcttg tagttgctga acttgctgcc tactaccgtt ctcgtggttt gacacttgct
     8401 gacggtatcg aagaaatcta taaagaatat ggctactacg cagaaaaaac aatctctgtt
     8461 actctttcag gtgtcgatgg tgctgaacaa atcaaagcga ttatggctaa attccgcaac
     8521 aatgctccaa cagaatggaa cgcaacagct atcactgtcg tagaagactt caaggcacaa
     8581 actgctactg ttgctgacgg tactgttaca aacttgacaa ctcctccaag tgatgtgttg
     8641 aaatacacac ttgctgacgg ttcatggatt gccgttcgcc cttcaggtac agaaccaaaa
     8701 atcaagttct acattgcagt tgtaggggaa accaacgaag aatcacaagc taagattgct
     8761 aacatcgaag cagaaatcaa tgcatttgta aaataaagac ctataaaaga tgagaccaac
     8821 caacctcatc ttttttcgta tcaaatctaa gcaattttag aaactttatg gcatactaga
     8881 cttatcaatg aaagcgaggt aatctcatgg aacaattttt agataacatc aaagaccttg
     8941 aagtcactac agttgtgcgt gcgcaagaag ctcttgataa aaaagaaact gcaaccttct
     9001 ttatcggtcg caaaacttgc ccttactgcc gtaaatttgc aggtacattg tcaggtgtcg
     9061 tagctgaaac caaagctcac atttacttca tcaatagtga agaaccaagc caactcaatg
     9121 atttgcaagc attccgctca cgctatggaa tcccaactgt accaggtttt gttcatatta
     9181 cagatggaca aatcaacgtc cgttgcgact cttcaatgtc agcacaagaa atcaaagatt
     9241 tcgcaggatt gtaaaaaatc agctaacaag tcttaaaatc acaaaaacgc ataatgtaag
     9301 gtgcaaaaac cttgatatta tgcgttttat tgttcctaaa acagttacat cagtatttca
     9361 ttacagaaca ttttaatttt ggaagtcaaa tcaattttta acaatgtttt agaaatcaca
     9421 gtgggctatt ccatattcaa ttatccatat tcaatcgcta ttatcgtttt cctagattac
     9481 aaaaaaaact acgttttgct aatcaaaacg cagtttcagc cactatttta tcttactgtc
     9541 cttcttttac ttctttggtt gctacatctt ctccaaacca tttttggctg atttcttgga
     9601 acttgccgtc cttgtaaaga ctagaaaaag cttcatttat cttcttaacc aagtttgtat
     9661 cttccttacg ggctccaacc gcaaaagctt ctgtttctag tccaactgta aagacattat
     9721 aatcgtttaa aacaccttct gcttctaaat aatagtttgc atagacacgg tcaatcaata
     9781 gaccatcaat tcgatcgttt ttcaaatcaa tcaaggcttc attaaaggtt tggtattgat
     9841 tcgcttcctt attagcgaca atattcttca aaatttctgg atttgcttca aagtccgcat
     9901 aaccagatga accagcttga gctcctaatg tctttccagt catatccttt gcagtcgtga
     9961 taccagatga tttcttcgta accaatacct gctcattctt catatatgag ttactgaaag
    10021 ccaccttttc acggcgttcg tctgtagcgg aatagccatt ccaaatcaga tcaatcgttc
    10081 cttttgtcaa ttcagcttct ttcaaatccc aatcaatcgg ttgccaattt accgtgattc
    10141 cgtatttttc aaaaacagct gtagctaaat caatatcaaa tcctgcataa gaaccatctt
    10201 tctgagcaaa tcccattgga acaaaagtac tatcaaatcc aatagtaata gacttgttag
    10261 actggtactt tgaccaatta tctccactag tttcgctaga atttttccca caagctacta
    10321 agaacaaagc agtcatcaga ctgactaata caagcatcca ttttttcatc catttttcct
    10381 cctatttagg ttctactttc aataatacat ctgcgatatt ttcagcaaac tgcaaatcat
    10441 gggtaaccac aatctgggtc atcccaagtt ccctattttg caagattagc ttctccactt
    10501 ccaaacgtaa ttctggatcc agggcagaag ttggttcatc gtagccaatg atttctgggt
    10561 caatcatcat agcacgcgcc aaagccaccc gctgcttttg cccaccagat agtgagaaag
    10621 gataggcctc tgcgtgtcct cctagtccta actgttccaa gagtccactc gccttcttct
    10681 cagcctcttc ctgcttcatt cccatggtct tcacaggcga taaagtcaaa ttttccagaa
    10741 ctgatagatg aggaaatagt tgaaaatctt ggaagacaaa tcccagtaga ttgcgcttct
    10801 gcaattcatc cagctctaaa ggttgtccat tataaaagat ttgccctgaa tcaatggttt
    10861 caagacctgc aagcatacgt aaaagagttg tcttacctcc accagaaggt ccaacgatag
    10921 ccaggatttg cttttcagga atacttagac tgaaattaga caggatttgt ttgtctccaa
    10981 agactttatt gatatttcgt aattctaaca tggcagcctc ctatctataa taactatact
    11041 tcttctcaac ttttttggaa ataattgtca caatcccaat caaaatcaaa taaatggctc
    11101 ctgccaagaa cataggaact agactagcat cgcggttagc agctgttcga ctagccaaga
    11161 taaggtctga aattccgaga gcatagacca aagaagtatc cttgaccaaa ctcataactt
    11221 cattaaagac actaggaaga acgatcttgg tcacttgggg caagataata tagcgcactc
    11281 tgtcaaaagg gctaaacttc aagaccttgg cggcctcata ctgtcctctt ggaatagtgt
    11341 caattccccc acggaaaatt tctgcaaagt aagctgcata gttgagaaca aaggcaataa
    11401 tagctgcagg aaggcggtct aaacgaatcc caatacttgg gagcacataa tagataaaaa
    11461 tcagttgcaa gagtaagggg gttcctcgca taacccagat gtaaatgtta atcagataat
    11521 ggaggggctt ccaatggact tgcaaggcaa aggcaatcaa aacgcccaag ggaatcgaaa
    11581 atagcaagac cagtgcaaag acctgtacag tcgtgctcgc accattcagt aaactcggta
    11641 atatctcaaa taaataagac atactgcacc tcctaaaagt aattgttcct attatagcat
    11701 gaataaacaa gattaccaag tatttttgta aaaagattct accatataca aataaaaacc
    11761 gtagctatca aaagagataa actacagttc tatatgttt
//
LOCUS       DAVHEX010000061        11776 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVHEX010000061 DAVHEX010000000
VERSION     DAVHEX010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11776
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.061"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..847
                     /locus_tag="VVL45_001763"
     CDS             <1..847
                     /locus_tag="VVL45_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU4187903.1"
                     /translation="KINNKLVGERIQNIRLSHGDSMEKFGEKFNTSKGTVNNWEKGRN
                     LPNKENLLKIASIGKMSVEELLYGDYNTYLHLKIMDLAPECIKNYDEYNSLHDDITNK
                     ALQIAQNTISKIDYQISDETIKKFIDLAIEQSRDLQGNLLKNNLSNFDYSLFGGTRNR
                     EYFYFGYRLTYKEKYLYTALLAILNESTKTIYIDDINTARFFNFFGNDIPNSSLKKID
                     YIAPSEIVSFSTYVRQRSKVIPKILEHILKSSFLLENIDVSGYTVNILEDQLTKHRTI
                     KISKN"
     gene            922..2085
                     /locus_tag="VVL45_001764"
     CDS             922..2085
                     /locus_tag="VVL45_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEU4187904.1"
                     /translation="MNIKEKIKKNGQRVYYASVYLGVDQLTGKKARTTVTATTKKGVK
                     VKARDAINTFAANGYTVKDKPTITTYNELVKVWWDSYKNTVKPNTRQSMEGLVRVHLL
                     PVFGDYKLSKLTTPILQQQVNKWADKANKGEKGAFANYSLLHNMNKRILKYGVAIQVI
                     QYNPANDVIVPRKQQKEKAAVKYLDNKELKQFLDYLDALDQSNYENLFDVVLYKTLLA
                     TGCRISEALALEWSDIDLESGVISINKTLNRYQEINSPKSSAGYRDIPIDKATLLLLK
                     QYKNRQQIQSWKLGRSETVVFSVFTEKYAYACNLRKRLNKHFDAAGVTNVSFHGFRHT
                     HTTMMLYAQVSPKDVQYRLGHSNLMITENTYWHTNQENAKKAVSNYETAINNL"
     gene            complement(2239..3543)
                     /gene="eno"
                     /locus_tag="VVL45_001765"
     CDS             complement(2239..3543)
                     /gene="eno"
                     /locus_tag="VVL45_001765"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEU4187905.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            3707..4153
                     /locus_tag="VVL45_001766"
     CDS             3707..4153
                     /locus_tag="VVL45_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEU4187906.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(4150..5265)
                     /locus_tag="VVL45_001767"
     CDS             complement(4150..5265)
                     /locus_tag="VVL45_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEU4187907.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIGVGGTASNDGGLGIAAGLGYQFYDRDGNVLP
                     ASGQSLLNLASVSTENCYKIPEGVQIHILADVVSPLCGHQGATYTFGNQKGLHPTMFA
                     VVDQAIQDFYEKFSPATLEIKGAGAGGGLAGGLCAFAQASIVSGIDTCLDLINFDKKV
                     SDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAEDLPPLPFENIQAV
                     FSILEKSEPLEDSLKNASFYLERTAANIGHLLNMCKI"
     gene            complement(5274..5507)
                     /locus_tag="VVL45_001768"
     CDS             complement(5274..5507)
                     /locus_tag="VVL45_001768"
                     /inference="COORDINATES: protein motif:HMM:NF019887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEU4187908.1"
                     /translation="MISPQVKKETLEKWRKKLKLSKERTVAIGDGVNNLLMLKSAKLG
                     IAFCAKEVLKKEIPHHVDKRDFLEVLPLIDCLE"
     gene            complement(5548..5919)
                     /locus_tag="VVL45_001769"
     CDS             complement(5548..5919)
                     /locus_tag="VVL45_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEU4187909.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVSGGF
                     TPIVGEISKIPWYCLFHCQLA"
     gene            complement(5978..7411)
                     /gene="glgA"
                     /locus_tag="VVL45_001770"
     CDS             complement(5978..7411)
                     /gene="glgA"
                     /locus_tag="VVL45_001770"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEU4187910.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGFERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(7408..8547)
                     /gene="glgD"
                     /locus_tag="VVL45_001771"
     CDS             complement(7408..8547)
                     /gene="glgD"
                     /locus_tag="VVL45_001771"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEU4187911.1"
                     /translation="MKIDKYSAILGNTVGFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVEHGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(8537..9679)
                     /locus_tag="VVL45_001772"
     CDS             complement(8537..9679)
                     /locus_tag="VVL45_001772"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEU4187912.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIINTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(9669..11597)
                     /gene="glgB"
                     /locus_tag="VVL45_001773"
     CDS             complement(9669..11597)
                     /gene="glgB"
                     /locus_tag="VVL45_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEU4187913.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAIVTELPEKKWKDGLWLARRKRWGFEERPVNIYEVHAGSWKRNSDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDDAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSAIKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFASQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
BASE COUNT         3701 a         2500 c         2070 g         3505 t
ORIGIN      
        1 gaaaataaat aataaactcg ttggagaacg tattcaaaat atccgtttaa gccatggcga
       61 ctctatggaa aaatttggag aaaaatttaa tactagcaaa ggtacagtta acaactggga
      121 aaaaggtcgc aatttaccaa ataaagaaaa cctactaaaa attgcatcta ttggaaaaat
      181 gagtgttgaa gagttactct acggcgatta caatacttat ctacacttaa agattatgga
      241 tttagctcct gaatgtataa aaaattatga tgagtataac tctttacacg atgatataac
      301 aaataaagcg ttacagatcg ctcaaaatac catttctaag attgattatc aaatttcaga
      361 cgaaacgatc aaaaaattta ttgatttagc tatcgaacaa tcgagagatt tgcaaggaaa
      421 tttgttgaaa aataacttat ctaattttga ctattcactt tttggaggta ctagaaaccg
      481 agaatatttc tattttgggt atagacttac ctataaagaa aaatatctat acactgcctt
      541 actagctata ctgaacgagt caacaaaaac gatatatatt gatgatataa atacagcaag
      601 attttttaac ttctttggca atgatattcc taattcgtct ttaaaaaaaa ttgactatat
      661 cgcaccttca gaaattgttt catttagtac gtacgttcga caacgttcta aagtaattcc
      721 taaaattttg gaacatatat taaaatcaag ttttttatta gagaatatag atgtttctgg
      781 ttacactgta aatattttag aagatcaatt aacaaaacat agaacaatca aaattagtaa
      841 aaactaactg gttgatctca tgtataaata cctaacaaaa ccacgcgcct tgcctgctga
      901 tggaaagaaa ggtacaaata catgaatatc aaagaaaaaa tcaaaaagaa tggccaaaga
      961 gtttattatg ctagtgttta tctaggcgtt gaccaactaa cgggcaaaaa agcccgtaca
     1021 actgttacag caaccactaa aaagggcgtt aaagtaaaag cgcgtgatgc gatcaatact
     1081 tttgctgcta atggctatac agttaaagac aagccgacaa ttacaacata taatgagctt
     1141 gtaaaagttt ggtgggatag ttacaagaat acagttaagc caaatactcg ccaatccatg
     1201 gagggattgg ttagagtgca tttattgcct gtatttggcg attacaagct atctaaactt
     1261 actacgccta ttcttcaaca gcaagtaaac aaatgggctg acaaggcaaa taaaggcgaa
     1321 aaaggggcat ttgctaacta ctctttgctc cataacatga ataagcgtat tttgaaatat
     1381 ggcgtagcta tccaggtaat acaatacaac ccagctaatg atgtcatcgt tccacgcaaa
     1441 cagcaaaaag aaaaggctgc tgtcaaatac ttagacaaca aagaattaaa acagtttctt
     1501 gattatttag atgctctgga tcaatcaaat tatgagaact tatttgatgt tgttctgtat
     1561 aagactttat tggccactgg ttgccgtatt agtgaggctc tggctcttga atggtctgat
     1621 attgacctag aaagcggtgt tatcagcatc aataagacac taaaccgcta tcaggaaata
     1681 aactcaccta aatcaagcgc tggttatcgt gatataccaa tagacaaagc cacattactt
     1741 ttactgaaac aatacaaaaa ccgtcaacaa attcagtctt ggaaattagg ccgatctgaa
     1801 acagttgtat tctctgtatt tacggagaaa tatgcttatg cttgtaactt acgcaaacgc
     1861 ctaaataagc attttgatgc tgctggagta actaacgtat catttcatgg tttccgccat
     1921 acacatacta ctatgatgct ctatgctcag gttagcccga aagatgttca gtatagatta
     1981 ggccactcta atttaatgat cactgaaaat acttactggc atactaacca agagaatgca
     2041 aaaaaagccg tctcaaatta tgaaacagct atcaacaatt tataaaaaat aagggtgacc
     2101 catttccggg ctaccctctt actataccaa aaattagtag gggtagtaaa aagggtatta
     2161 aattataaaa agcactaagg gaaagcgccc caaagtgctt atttcaaagg ctttatagcc
     2221 tataatcaca taaagagatt attttttaag gttgtagaat gatttcaatc cacgatattc
     2281 agctacttca ccaagttggt cttcgatacg aagcaattgg ttgtatttag cgatgcggtc
     2341 tgtacgtgaa agtgaaccag tcttgatttg tcctgcgtta gttgcaactg caatatcagc
     2401 gattgttgaa tcttcagttt cacctgaacg gtgtgataca acagcagtgt aaccagcttc
     2461 tttagccatt tcgatagctt caaaagtttc agtaagagta ccgatttggt taactttgat
     2521 aaggattgag ttagcagcac cttcttggat accacgtgca aggtagtcag tgtttgttac
     2581 gaagaagtcg tcaccaacaa gttgtacttt cttaccaaga cgttcagtaa gagctttcca
     2641 accatcccag tcgttttcat ccataccatc ttcaatagtg atgattgggt atttgttaac
     2701 caattcttca aggtagtcga tttgttctgc agatgtacga acagcagcac cttcaccttc
     2761 aaatttagtg tagtcgtaaa ctttacgttc tttatcgtag aattctgatg aagcacagtc
     2821 aaatccgata aatacgtctt tacctggtac atatccagca gcttcaatcg cagcaaggat
     2881 agtttcaaca ccatcttcag ttccttcgaa acgaggagcg aatccacctt cgtcacctac
     2941 ggcagtttcc aaaccacgtg atttaaggat tttcttaaga gcgtggaaga tttcagcacc
     3001 gtaacgaagg gcttctttaa atgttggcgc accaactggc aagatcatga actcttggaa
     3061 agcgattgga gcgtcagagt gagaaccacc gttgatgatg ttcatcattg gagttggaag
     3121 aactttagtg ttgaatccac caagatagct gtaaagtggg atttcaaggt agtcagcagc
     3181 agcacgagct acagcgatag acacaccgag gattgcattc gcacccaatt tacctttgtt
     3241 aggagtaccg tcaagtgcga tcatagcacg gtcaatagct tgttgatcac gtacatcgta
     3301 gccaatgata gcttcagcaa tgatgttgtt tacgttgtca acagcttttt gtgtaccaag
     3361 accaccgtaa cgagatttgt caccgtcgcg aagttcaact gcttcgtgtt caccagtaga
     3421 agctcctgat ggaaccatac cacgtccgaa agcacctgat tcagtgtaaa cttctacttc
     3481 aagtgttggg ttaccgcgtg agtctaggac ttcgcgagcg taaacatcag taataattga
     3541 cattttttac tctccttatg agttaaattt tttacacctc tataatacct taaaacccct
     3601 cctttttcaa gaaaaaacgt tatctttgtg caacttttcc ttaactttat aaagtaatcg
     3661 ctttcttttg tctgttttat tctaactttt atgatatact gttttcatga cagatttatc
     3721 aaaacaatta cttgaaaaag ctcatggtgg gttaaaaata aatccggatg agcaaagacg
     3781 ctatcttggt acttttgagg aaagagttct tggatatgta gatattgaca cagcaaatag
     3841 ccctcagtta gaaaaaggct ttttatttat tttagaaaac cttcaggaaa aagcagagcc
     3901 actatttgtg aagatttcac caactatcga atttgataag caagttttct acttaaaaga
     3961 agcaaaagaa actgatagtc aagccaccat agtatctgaa gagcatatta cttctccttt
     4021 tggcctggtt attcatagca atgcaccagt tcaagtagaa gaaaaagacc ttcgacttgc
     4081 ttttccaaaa ctttgggaag ttaaaaagga agaaccagcc aaaacatcct tatggaagaa
     4141 atggtttagc taaatcttgc acatatttaa taagtgccca atattggcag ccgtgcgctc
     4201 cagatagaaa ctggcatttt tcaaactatc ttctaaaggt tcacttttct ccaaaataga
     4261 aaagacagct tggatatttt caaatggtag gggaggtaaa tcttcagcaa gactaccgca
     4321 aatagcaata acaggaactc caacaggggt tctttttgca acacctatag gcgctttccc
     4381 agcaaagctt tgactatcaa gtcttccttc tccaacaaca accaagtcag catctgaaac
     4441 tttcttatca aagttgatta agtccaagca ggtatcaatt ccagacacga tacttgcctg
     4501 agcaaaggca cacaaaccac cagcaaggcc tccacctgct cctgctcctt taatttctaa
     4561 tgttgcaggt gagaattttt cataaaaatc ttggatcgcc tgatctacga ctgcaaacat
     4621 agtcggatgt agaccttttt gattgccaaa agtgtaagtc gcaccttgat gaccacataa
     4681 gggactcacg acatctgcta aaatatgaat ttgaacacct tcaggaattt tatagcaatt
     4741 ttctgttgaa acagaagcta agtttaataa ggattgaccg gaagcaggca agacatttcc
     4801 atccctatca taaaattgat aacctaaacc agcagcaatc cccagtcctc catcattact
     4861 ggccgtgcca ccaacaccga tataaatatc tttaatccct ttagagatga gatgaagaat
     4921 caactctcca ataccacaag tttggatttg aagtggattt cgtttctcta gcggaatttt
     4981 tccaagacca accaagtcag ctacttcaaa tagtgccagt tccccttttt gaaaatagcg
     5041 catggcttct ttttgtccaa aagggtctgt cacttggatc catttttctt ttaggtcaag
     5101 agaatgtcgg atagcatcta cagtaccttc tcccccatca ccaacagggc agaggagaca
     5161 ttctacatct gctatcgatt gttggaagcc tctttttatt gcttcagcta cctgttgagc
     5221 tgtcaagctt tccttaaacg aatccggtgc aattacaatc ttcatatttt ccctcattct
     5281 aaacagtcaa tcaaagggag aacttctaaa aaatccctct tgtcaacatg atgtggtatt
     5341 tcttttttga gcacttcttt ggcacaaaag gcgattccta acttcgccga cttcaacatt
     5401 aatagattat taaccccatc accgattgcc accgttcttt ctttagaaag ttttagtttc
     5461 tttctccatt tttccagagt ctcttttttg acctggggac ttataatttg tccaactaat
     5521 tttcctgtta aaagaccttc tttgacttca agctagttgg cagtgaaata ggcaatacca
     5581 agggattttg ctaatctctc caactattgg tgtaaatcca ccagacacca gaccaactag
     5641 gatgccattc ttttggagaa tagagatgaa ctctgggaca tttagcgata gatgaattga
     5701 gttgaagacg ttatcaaaga ccaaaatagg aagaccttcc aacaaggaca ctctttttct
     5761 taaactgctt tcaaagacca actctcctcg cattgctcga cttgtaatct gcgaaatttc
     5821 cgcctcatga cctgcctctc tccctaaaag atcaatcact tcttctagga ttaaggttcc
     5881 atctacatcc aaaacacaca agccttttac ttgagacatc agttctcctc tctaaacagc
     5941 ctaaaaatcg tatgaagtca tcatacgatt ttatctatta attaactaaa ctatggtaca
     6001 agtcaaggta tgacttgcag gctgtatccc atgagaagtc actctccata gcttgttttt
     6061 gtaggtttct ccaaatgtct ggatggtttc tatacaagtc caatgctgtt tggaaagtcc
     6121 aatttaacca ataaggagat agattgtcaa agctaaagcc agtaccgctt ccttcgattg
     6181 gattgaaagc gcgaactgta tctcgcaagc ctccaacttc atggaccaat ggcaaggttc
     6241 cataacgcat agccatcatt tgagacaagc cacacggttc aaaacgactt ggcatgagga
     6301 agaggtcaca agcagcgtag atttcctgag caagtttgac atcaaaagtg atatttgttg
     6361 atagcttgtc tgggtaaatc tgagcaaacc atgagaaagc tccttcaaag gctggatcgc
     6421 cagttcccaa aagaacaatc tgaacatctt cttgcaagat atggtgaaga ctttcgacca
     6481 ccacatcaaa acctttttga cgtgtcaaac gagaaacaat tcccaccagt ggaacgtctg
     6541 ctctaacagg caagccaact ctttcttgca attttgcctt atttttggct ttcccagaca
     6601 aatcttcctg attgaaatga tagtctaaaa gagcatccgt ctgaggatta taaagatcag
     6661 catcaatccc attcacgata ccagatactt taccagactc cattttaaga atctgatcca
     6721 aattacatcc aaactgacta gtcataattt catgagcata gctaggtgaa acggttgaaa
     6781 cacggttcgc atagagaata cctgccttca tccagttcag acagttgttc catcgaaggg
     6841 tgccatcagc gtaacgttca aagccaactc caaacaaatc acccaacatt ccttctgaaa
     6901 attgtccttg gaattctaaa ttatgaatgg ttaaaactgt ttcaatgtcc tcataggctt
     6961 gaatccaacg gtatttttcc ttcaacaaga aaggaatcat agctgtatgg tagtcatgaa
     7021 catggagaag atcaggaata aagtcaatcc tttccatagc ctcaatggca gccagttgga
     7081 aaaaggcaaa gcgttctccg tcatcaaaat caccgtaaac atgaccacgg aagaaataat
     7141 attgattgtc aataaagtag aaggttacac catttaatac tgttttctta attccacaat
     7201 actgtctgcg ccaaccaacg ctcacctcaa aatgaagcac atcttcaatc tgatttccaa
     7261 atttagcctc taccatatca tagtagggta aaatcactgc aacttcgtgc ccagctttta
     7321 ccagtgattt tggaagagcg ccaatgacgt ctcccaaacc acctgttttt gaaaagggtg
     7381 caccctctgc tgctacaaat aaaattttca tgaatgaata tcctctgtta ctttagcacc
     7441 tttcttaacc acaactggat gttctgcagt tcctcgaatc acaacaccat gctcaacttc
     7501 aacccctttg tccaagatag catattcgac ctgagcccct tctccaataa caacacgagg
     7561 gaagagcagg ctatctttaa ccaagctatc cttatggaca tgaatattac gtgatagaac
     7621 agaattagct acttgacctt caataatact accagaggca aactgagaag tgcttacctt
     7681 agatgtatta gcatagtaag ttggctcttc gtttttgacc tttgtataaa tcttttggtt
     7741 tggtgagaaa agagaataga atttttgtga ttcaagcata tcgatattcg cttgataata
     7801 agatttaaca gagtgaatat tggctagata gcccgtgtac tcgtaggcga aagctccctc
     7861 ttttacagcc aaatcccgta aaacatagcg caatttctct ggatgttctt ttttagcttc
     7921 ttcttccaag tgttcaatca accaaggtgt atcaacgaca aagatatctg tagacatatt
     7981 gaacgtttca gctgttgact tgctatcaaa gagtttatga gaaagaacat ggtctgtttc
     8041 atctacatcc aagattgcat ttacttctga aatatctttc ttagctagtt ttttataaac
     8101 tacagtgata ggctcttttg ttgtactatg taggtggaaa acttggttca aatcaatgtt
     8161 aataagaaca tcgcagttga gggcaaccgt ttggtttgag ccagaacgtt tcaaataagt
     8221 aagaagctgt tggtagtatt cttttccaac tgtactactt tctacacggg tattgtaaat
     8281 tcctagatag taatggctaa gaagggttga taagccccac tcgcgtcctg aacgaatatg
     8341 gtcaaatact gagctgatat tatcctgctg gaaaatacca aagacactac gaacacctgc
     8401 attagcaagg cttgaaagtg ggaagtcaat caaacgatat ttcccaccaa atggcaaact
     8461 tgctactgga cggtggtccg tcaatgtcga catattgtga aaaccaactg tatttcctaa
     8521 aatggcagaa tatttatcaa tcttcatctg ttgctacccc cactacttca ttatatccta
     8581 caacttgtac ttcatctgtt ccatcaattt cgacaccgtc agaaataatc gcaccttcac
     8641 caataatggc acgtttaatc ttagctcctt gaccaatgat agctccactc atgataactg
     8701 aatcaaggac ttccgctcct tcgcgaactt gcgcgcctgt tgaaaggata gaatgtttaa
     8761 cagttccatc aacgaaacat ccgtctacaa ctaatgagtc ttccacatga gcatttgccc
     8821 cgaggaagtt tggtggtgaa atcaagtttc ttgagtaaat cttccattga cggttacgac
     8881 tatccaaggc attttctgga gaaatatact ccatgttcgc ttcccaaagt gactcaatag
     8941 taccaacatc tttccaataa ccactaaatt cgtaagcata aacactttca cctgactcaa
     9001 ggtaatttgg aatgacattt ttaccaaagt ctgacatgcc aaccttgctc ttttcagcag
     9061 cgactaacat attacgaagg cgttgccaat caaaaatgta gattcccata gaagcttttg
     9121 tagatttagg ttgagctggt ttttcttcaa attcaacaat acgattgtta gcatctgtgt
     9181 ttatgatacc aaaacggctt gcttctttaa gagggacgtc taaaactgct actgtcaagc
     9241 tggcattatt atccttatga gactggagca tatcatcata gtccattttg tagatgtgat
     9301 ccccagacaa aatcaagaca tactcaggat tgacactgtc gatatagtcg atattttggt
     9361 aaatagcgtg actagtcccc tcaaaccaac gatttccttc acttgcagaa taaggttgaa
     9421 gaatagagac acctgaatta ataccgtcta gtccccagct tgaaccattc ccaatatggt
     9481 tgttgagagc aagtggttga tactgtgtaa cgaccccaac attgtgaatc cctgagttgg
     9541 cacagtttga tagggcaaag tcaatgatac ggtagcgccc accaaattgc acagctggtt
     9601 ttgcgatgct ttgagtgagt ttaccgagac gagttccttg cccaccagca agaatcaaag
     9661 ctaacatttc atttttcatt ttctactcct ttttggtttt tatttgtgac ggttttagta
     9721 gatttcaagc gacgtttgat tttccataca cttgctccca tagccggtag ggtaaaggtt
     9781 aaggtctgct cataatcttt ccatagtcct tcttgcgttt gaacagtttg attatgttct
     9841 ttccaaacgc ctccccactc ttccaactca gtattccata cttcttcgta aattcctgca
     9901 acgggtagtc cgattgtaaa atctttccgc tcaacaggta ccatattaaa gatacagact
     9961 aacatttctc cctttttacc cttacgaata aaggaaagaa cactctggtc tcgattatcc
    10021 gcatcaatga tttcaatacc atcatagctg gtatcaattt cccacagaca gcgatgatct
    10081 ttgtaaaact ggtttagctg agaagcgaaa tacttcatct tagcattcat tgggtcttct
    10141 aggttagacc attccaactg ttcttcagat ttccattcta ggaattgacc gtattcgcta
    10201 cccatgaaga gcaatttctt accagggtga caaatttggt acgtatagag attgcgcaag
    10261 cctgcgaatt gattgtaacg atctccccac atcttatgca tcatactctt cttgccatga
    10321 accacttcat cgtgcgagaa tggcaagaga taattctcct tgaaaacata cataaagctg
    10381 aaagtcacca ggttaaagtc atatttacga tagatcggat cttcttcgta gaaacggagg
    10441 atatcattca tccagcccat gttccatttg tagtcaaatc ctagaccacc aatctctttc
    10501 attcccgtaa tcttgatcgc agacgaactt tcttctgcaa tcatcatcac atctggatat
    10561 tctaacttaa taacctcatt caagcgctga aggaaataat aaccttcata gttgagattt
    10621 ccgccatctt tattaggtgt ccatggagca tcatcatagt ccaaatagag catgttgcta
    10681 acagcatcca cacgaatacc atccaaatga tagacatcaa tccaatgctt aatgcaagaa
    10741 attaagaagg actggacttc attttttcca aggtcaaaat taagggcacc ccaaccatgg
    10801 ttatgagcct tattatggtc ttggtattca aaagtcggtg tcccatcata ataggctaag
    10861 gcatcatcgt tgatggtaaa gtgacctggt acccagtcca caataacccc aatattatgg
    10921 gtatgacact cctcgacaaa atcttgaaac tcctctggtc ggccataagc atgctctaaa
    10981 gcgaagtaac ccataagctg atacccccaa ctcaagccca aaggatggga catcaagggc
    11041 ataaactcaa tatgagtata gttcatttca acgagataag gaatgagttc atccttgagc
    11101 tgggcaaaac tataaggact gccatcagaa tttcttttcc atgatccagc gtgaacttca
    11161 taaatattga caggacgctc ttcaaagccc caacgttttc ttcgtgccag ccaaagtcca
    11221 tccttccatt tcttctcagg aagctctgtt acgattgccc ctgttcctgg acgagcctca
    11281 tacctgacag caaaagggtc aatcttcatc agttgatgac cattttgacg tgtgacatga
    11341 tatttgtaaa tatgcccttc ttgagccata ttggtaaaga cttcccagac cccaaaatca
    11401 tttcttacca ttggaatctg attttcaatc cagttggtaa aatcaccaac caagtgaaca
    11461 gcctgagcat taggtgccca aacacggaag gtatagccat gctctccatt tagttcttcc
    11521 ctatgtgctc ctagataatg ttggagataa aaattttcac ccgtcataaa ggtttttaat
    11581 gcttctctat tatccatata ctccccttct cctgtaagcg ttttctatgt ttttattata
    11641 ctaccttttt agagaagatt caagtaaatt actatacttc tttaattatt ttgaaaatct
    11701 acaacaagtt cacttactcg ttcaattgta aatcaatatt ttttcaaaaa attgcgaaaa
    11761 cgcctttctt tttcta
//
LOCUS       DAVHEX010000062        11753 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVHEX010000062 DAVHEX010000000
VERSION     DAVHEX010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11753
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.062"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..776
                     /locus_tag="VVL45_001774"
     CDS             <1..776
                     /locus_tag="VVL45_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU4187914.1"
                     /translation="DSFPLIWFHTLLDKVLRTCKEFGVNAIVEYFGEEDTISNSIISS
                     TGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSGVVYVSNNNFQATYDMMKA
                     VTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDLKVIYGLSTIDSVYSYAMQ
                     HLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISIIGFDNIPISQYYTPALSTI
                     APNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
     gene            1378..1458
                     /locus_tag="VVL45_001775"
                     /pseudo
     CDS             1378..1458
                     /locus_tag="VVL45_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            2097..3458
                     /gene="trpE"
                     /locus_tag="VVL45_001776"
     CDS             2097..3458
                     /gene="trpE"
                     /locus_tag="VVL45_001776"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEU4187915.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIVPHKFEEMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKALATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMEVELFRY
                     VMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKTKSMTRIGE
                     LRP"
     gene            3455..4021
                     /locus_tag="VVL45_001777"
     CDS             3455..4021
                     /locus_tag="VVL45_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEU4187916.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMIRDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGRAVMRYHSILIEEMPEDFEVTARSTDDQTIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            4032..5036
                     /gene="trpD"
                     /locus_tag="VVL45_001778"
     CDS             4032..5036
                     /gene="trpD"
                     /locus_tag="VVL45_001778"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEU4187917.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            5033..5800
                     /gene="trpC"
                     /locus_tag="VVL45_001779"
     CDS             5033..5800
                     /gene="trpC"
                     /locus_tag="VVL45_001779"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEU4187918.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDR
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALAEERLKELYDYATVLGLEVLV
                     ETHNLAELEVAHRLGAQIIGVNNRNLTTFEVDLQTSVDLAKHFKDDCLYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAEDVAQRIKELQIDKG"
     gene            5787..6386
                     /locus_tag="VVL45_001780"
     CDS             5787..6386
                     /locus_tag="VVL45_001780"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEU4187919.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEVAE
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVADDLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQPFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK"
     gene            6364..7587
                     /gene="trpB"
                     /locus_tag="VVL45_001781"
     CDS             6364..7587
                     /gene="trpB"
                     /locus_tag="VVL45_001781"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEU4187920.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDMTGQNLPDALVACVGGGSNAIGLFHPFVEDESVAMYGAEAAGLGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            7580..8356
                     /locus_tag="VVL45_001782"
     CDS             7580..8356
                     /locus_tag="VVL45_001782"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEU4187921.1"
                     /translation="MPKTLTEKLNAIKATGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DFGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFIYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIKQAVAYQK"
     gene            complement(8328..8429)
                     /locus_tag="VVL45_001783"
                     /pseudo
     CDS             complement(8328..8429)
                     /locus_tag="VVL45_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            8658..9389
                     /locus_tag="VVL45_001784"
     CDS             8658..9389
                     /locus_tag="VVL45_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187922.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WTRPNHIDTAFASADLFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFP
                     LVQKNKSGYLFEYAQMNTLYLNQHSSYKNDEDSHSFPIQMELVSDEMIQEIEQATKNS
                     NIEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDI
                     PNFSELSETEDFEIIPRIEKQEGQK"
     gene            9404..10555
                     /locus_tag="VVL45_001785"
     CDS             9404..10555
                     /locus_tag="VVL45_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEU4187923.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESVRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNIIIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGNRLEQEANYFASCFLLPKEEFLVKFEERVGKRVSNPDSYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(10607..11266)
                     /locus_tag="VVL45_001786"
     CDS             complement(10607..11266)
                     /locus_tag="VVL45_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEU4187924.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTRLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELVLGLLFLLYSWELLSLGQVVLITAG
                     LTLGIYDFHHQEYPLLVWMTFHLILIASSGWNLVMVSFLALGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFLLQKKKERLPFVPFLLLAACGIIFGKLLL
                     V"
     gene            11336..>11753
                     /locus_tag="VVL45_001787"
     CDS             11336..>11753
                     /locus_tag="VVL45_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4187925.1"
                     /translation="MTIRFEEKVSIENAQFVCQWSNSLGKSFQEQWMGPRIPFLLTLQ
                     ALEGVFSIFDEQEFVGLIQKIRLEDSNLHIGRFFINPQKQEQDLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQMVEAPV"
BASE COUNT         3682 a         2094 c         2717 g         3260 t
ORIGIN      
        1 tcgactcttt tcctttaatt tggtttcata cgctgttaga taaagtgtta cggacgtgta
       61 aagaatttgg tgttaatgct atagttgagt actttggaga ggaggataca attagtaatt
      121 caattatatc aagcacaggt agcctagtag atggtgttat tgttttttat gaaagtgtag
      181 atgatatcag aattcagtat ttaaagaaga accatatgcc ttttcttgtt ttcggtgaat
      241 ctcaaacgtc tggagtagtc tatgtatcta ataataattt tcaagctact tatgatatga
      301 tgaaagctgt aactgaagaa aaatttaaaa atatgtggtt gcttatgggg ggagaatccc
      361 atgttaataa ggatcgtgaa agaggtgtgc gttcttttct gaatgataaa aattacttta
      421 tggatttaaa agtcatttat ggcttatcta caattgattc agtttattct tatgcaatgc
      481 agcacttgac tacccaaaat tatccggaca ttatttttgt ttcaggagat gaaaaggttc
      541 agggcttgat tcgtgcgtgc tatgagaaag gaattcttat tccagatgat atttctatca
      601 ttggatttga taatattcct atttctcagt attatactcc agctttgtct acaattgctc
      661 ccaattatgt taaattggct aaagaaatga tagaaggtgt tttagcaatt attaaagggg
      721 aaagtgtcac atctgttgaa gtttctccta aatttgttag gagacagagt ttctagtatt
      781 gtttaattct tgaaaatcct agtggaaaat atggagtgtt ctaaaataag ttctgtttag
      841 cctccattca gtttctattg agttggatcc aatttctaac aagtgggaca tgttcatgtt
      901 cgatctaagg aagacatggt catattcgaa tgatattttc aaaaaaatcg taaaaaagtc
      961 ttgacaaaga aaaaacaaag tattaaaata agttcaacaa atcagaaaag taactatttt
     1021 tgatcttcag ggagcctgtg gtgattgtga ataggtggtt ggaagtagtg aaagtgggct
     1081 gattttgaaa atgaatttga aacaatgaaa attcggtgcg cacaccttac agtgcagctt
     1141 gttgttaaac aaggcagaga tgtaaaggga tagtcccttt ataattgagg tggcaccgcg
     1201 ttaccaacgc cctcacacgg aagtatattc tgtgtgtggg cttttttcta tccgtcgttt
     1261 ggtttatctt ttattagggc cttaagtcgc tttgatgaac ttgagttcta tctacagctc
     1321 cttgcctact actaaaagca aacaaaaagg ctggactaat acgaaaaaag ctagtaatac
     1381 tcaatgaaaa tcaaagagca aactaagaaa ctagccgcag gttgctcaaa acactgtttt
     1441 gaggttgtgg atagaactga cgaagtcagc tcaaaatact gttttgaggt tgtggataga
     1501 actgacgaag tcagctcaaa acactgtttt gaggttgtgg atagaactga cgaagtcagc
     1561 tcaaaacact gttttgaggt tgcagataga actgacgaag tcagctcaaa acactgtttt
     1621 gaggttgcag atagaactga cgaagtcagt aaccatacct acggcaaggc gacgttgacg
     1681 cggtttgaag agattttcga agagtataaa ggtgtaccga gatgaacatc tcttcatcaa
     1741 tgtctctggt cgtggaggca aggacctagc tgcgattgca gattactaga agctaaaaaa
     1801 taatagatgg aaaaattaca gttctttctc tcacagagag cttgtggttg ctgaaaacaa
     1861 gcagagaaag ctgtaaagtt gggttcatct gaaacgagag aagaaaacat cattctcaag
     1921 ggagtgccct ttatcgcaca gcctgttaca ggagatatct gagacaggaa tgagagatag
     1981 gagaaatcct ataattgagg tggcaccgcg aatttcgtcc tcacgcaagt tattttgcgt
     2041 ggggattttt catacagtcg ctacgaacta gaattagaac tgaaagggaa attacaatgg
     2101 aacgaatcat tcatggagat gtcttatcac caatcttggc ttatatgcgc ttaaaggggc
     2161 aacataaggt tattttagag agtattccga gagacaagga aacggctcgt ttttctatcc
     2221 tagcctataa tccagttttt gagattaagt ttgaaaatgg agttctctat caaaatggtc
     2281 aagtgattga tcgggatccc ttggatttcc tttatgaagt gattcataag agtcagcacc
     2341 actcagagct accttttggt ggtggagcca ttggttttgt gggttacgat atgatttcgc
     2401 tttatgaaga aattggtcaa atccctgagg atacaattgg gacgccagac atgcatttct
     2461 ttgtctatga gagctatatg gtctttgacc acaagaagga gaagatacat gtcatagagg
     2521 atgcacttta tagtgagcgc agtcaagagg ccttggaaaa atccttgaac caagtgcttg
     2581 aggaattacg cattcctgct ccaaatgaat ttgaagactt ggatctatct ccgttagact
     2641 tcaaaccgca tatcgttcca cataagtttg aggaaatggt ggaaaccgct cgtgacttga
     2701 ttcgtaacgg cgatatgttc caatgtgtac tcagccagcg tttctcagca gaagttactg
     2761 gaaatccatt tgacttctac agaaatctcc gcgtaactaa tccttctaat tacctttatt
     2821 tctatgattt tggggattat caaatcatcg gagctagtcc agaaagtttg gtttctgtca
     2881 aaaatggcat cgtgacaacc aatccgattg cagggacgcg accaagaggg gctacggatg
     2941 aagaagacaa ggccttggcg actgacctcc tctctgatga gaaggaaaca gcagaacatc
     3001 gaatgttggt agacttgggg cgtaacgata ttggccgcat ctctgaaacg gccagtgtcc
     3061 aagttactaa gtatatggag gtggagctct tccgctacgt catgcatttg accagtgtgg
     3121 tcaagggacg tttgcttgca gaactcactg ccatggatgc cttgaaagca acgcttcctg
     3181 ctggaaccgt ttctggagca ccaaaaattc gagctatgag acgcatctat gaactggaaa
     3241 cggaaaaacg gggcgtatat gcaggagcaa tcggctactt gtctgcgacg ggtgatatgg
     3301 atttggccat tgccatccga accatgattc tcaaaaatca aagagcttat gtgcaggctg
     3361 gggcagggat tgtctacgac tctatcgccc aaaacgaata ccaagaaacc attaacaaaa
     3421 caaaatctat gactagaatt ggagaactaa gaccatgatt ttattgattg acaactatga
     3481 ttcttttacc tataacttgg cccaatacat tgggaatttt gcagaagttc aggttctgag
     3541 aaatgatgat tccaagctgt atgaagaagc tgaaaaagca gatggtctgg tcttttctcc
     3601 tggtcctggt tggccagttg atgctggaaa gatggaagac atgattcgtg attttgcagg
     3661 caagaagccg attctaggga tttgtttggg ccaccaagcc attgcagaag tctttggtgg
     3721 taagctaggt ttggctccaa aagtcatgca tgggaaacag agcaatatca actttgaagc
     3781 gccatctgtt ttgtatcaag gtattgagga tggacgtgcg gtcatgcgtt atcacagtat
     3841 tttgattgag gaaatgccag aagactttga agtgacagct cgttcgactg atgaccaaac
     3901 catcatgggg attcaacata aaaacctacc gatttatggc ttccagtacc atccagagag
     3961 cattggaacg ccagacggct tgtcttctat tcggaatttt atcgagaagg ttgtaaagtg
     4021 aggaaactag gatgaaagag attattgaaa aactagcaaa atttgaaaat ttatcaggtg
     4081 tggaaatgac ggatgtcatt gagcgtatcg taactgggcg tgtaactgaa gcgcagattg
     4141 cttctctcct cttagctctc aagatgaagg gggaaacacc tgaagaacgc acagccattg
     4201 cccaagtcat gagaggacat gcccagcata ttccaactga gattcatgat gccatggaca
     4261 actgtggtac aggtggggac aagtctttca gttttaatat ttccacaact gcagcctttg
     4321 tcttggctgg tggcggtatt cacatggcca agcatggtaa ccgctcgatt tcttctaaat
     4381 ctggttccgc agatgtcctc gaagccttgg gaatcaatct tgacctcaaa ccagctgaac
     4441 taggtaaggt ctttgataaa actggaatcg tctttctctt cgctaaaaat atgcacccag
     4501 ctatgaaata catcatgcca gctcgtttgg aattgggaat tccaacgatt atgaacttga
     4561 ctggtccact gattcatcca atggctttag aaacacagct tcttggaatt agtcgtccag
     4621 aactcctaga aagtacagct caggttttga aaaatatggg tcgcaaacgt gccatcgtgg
     4681 ttgctggacc agaagggttg gatgaagctg gcttgaacgg aacaaccaag attgcacttc
     4741 ttgaaaatgg cgaaatcagc ttgtcaagct ttactccaga ggatttggga atggaaggct
     4801 atgctatgga agatattcgt ggtgggaatg ctcaggaaaa tgcagaaatt ttgcttagcg
     4861 ttctgaaaaa cgaagcaagt ccattcttgg aaacgacagt cttgaatgct ggtcttggtt
     4921 tctatgctaa tggtaagatt gatagcatca aggaaggagt tgccttggcc cgtcaagtga
     4981 ttgctagagg caaggccctt gaaaaactca gactgttaca ggagtaccaa aaatgagtca
     5041 ggaattttta gcacgaatct tagagcagaa ggcgcgtgag gtggagcaga tgaagctgga
     5101 gcaaatccag cctctgcgcc agacctatcg cttggcagaa tttttgaaga atcatcagga
     5161 ccgcttgcag gtaatcgctg aggtcaagaa agctagccct agtttgggag atatcaatct
     5221 cgatgtggat attgtgcaac aggcccagac ttatgaagaa aacggagcag tgatgatttc
     5281 ggtgttgaca gatgaagttt tctttaaagg acatttggat tatctacggg agatttccag
     5341 tcaggtagag attccgacgc tcaacaagga ctttatcatc gatgaaaagc aaatcatccg
     5401 cgctcgcaat gcaggtgcga cagtcatctt gctcattgtg gcagccttgg cagaagaacg
     5461 tcttaaggaa ctttacgact acgcgacagt gcttggtctg gaagtcttgg tggaaactca
     5521 caatctagct gaactagaag tggctcacag acttggtgcc cagattatcg gggtcaataa
     5581 ccgcaacttg accacctttg aggtcgactt gcagaccagt gtagacttgg ctaaacattt
     5641 caaagatgat tgcttgtaca tttctgaatc tgctattttc acagggcagg atgcggaacg
     5701 actagcccca tactttaacg gaattttagt agggacagct ctcatgcagg cagaggatgt
     5761 ggcccagaga atcaaggagt tgcagattga caaaggttaa gatttgtgga ctatcgacca
     5821 aagaagcggt ggaaacagcc gtttcagcag gagccgacta catcggtttt gtcttcgcac
     5881 ctagtaaaag acaggtaact ttagaagaag tagctgagtt ggcaaagctt attcctgcag
     5941 atgtgaaaaa ggttggagta tttgtttcac caagtcgggt agaactgctg gaagcgattg
     6001 acaaagttgg cttggacttg gttcaagttc acggtcaggt ggcagatgat ttatttgaga
     6061 atttgccttg tgccagtatt caggctgtgc aggtagatgg aaatgggcat gtccccaatt
     6121 ctcaggcaga ttatctactc tttgatgccc ctgtggcagg aagtggccag ccctttgatt
     6181 ggggtcaact ggatacgact ggactagcac agcccttctt tatcgcaggt ggccttaatg
     6241 aagataatgt agtaaaagca attcaacatt ttactcccta tgcagtagat gtatcgagcg
     6301 gagtggagac agatggacaa aaagatcatg aaaagattag aagatttata gagagggtaa
     6361 agcatggcat atcaggaacc aaataaagat ggattttacg gaaaattcgg cggacgtttt
     6421 gtcccagaaa cattgatgac agcagttttg gagttggaga aggcctaccg tgaaagtcag
     6481 gcagacccaa gtttccaaga ggaattaaac cagcttttgc gccagtacgt aggacgtgaa
     6541 actcctcttt attacgcaaa aaacttgacc cagcatattg gcggagccaa gatttatctc
     6601 aaacgggaag accttaacca tacaggagcc cacaagatta acaatgcctt aggacaagtt
     6661 tggcttgcca aacgcatggg taaaaagaaa attatcgcag aaacgggtgc tggtcagcac
     6721 ggtgtggcaa ctgcaactgc tgcagccctc tttaacatgg aatgtaccat ctacatgggt
     6781 gaggaagatg tcaaacgcca agccctcaat gtcttccgca tggagctttt gggagccaag
     6841 gtcgaggccg tgacagatgg ttcgcgcgtg ctcaaggatg cggtcaatgc agcccttcgt
     6901 tcatgggtgg ctaatatcga cgatacccac tatatccttg gttctgcctt ggggcctcat
     6961 ccatttccag aaatcgttcg tgacttccaa agtgtcatcg gtcgcgaagc taaacaacag
     7021 tatcgcgata tgacaggtca aaatctgccc gatgccctag tagcctgtgt tggtggtggg
     7081 tcgaatgcca tcggtctctt ccatcccttt gtagaagatg agtcagtagc tatgtatgga
     7141 gctgaagcgg ctggacttgg tgtggataca gagcaccacg cagctacctt gaccaagggt
     7201 cgtccaggtg tccttcacgg ttccctcatg gatgtgctcc aagatgccca tggtcaaatt
     7261 cttgaagcct tctctatctc agcgggtttg gactatcctg gtatcggtcc agagcattct
     7321 cactaccacg atattaaacg tgccagctat gtccctgtga cagacgaaga agccttggaa
     7381 ggattccaac tcttgtctcg tgtggaaggg attatcccag ccttggaatc tagccatgct
     7441 atcgcttttg cggtgaaatt ggccaaagaa cttggaccag aaaagtctat gattgtctgt
     7501 ctatcaggtc gtggggacaa ggatgtggtt caagtcaaag accgcttgga agcagatgca
     7561 gcaaagaagg gagaagctca tgcctaagac actaacagaa aaattgaatg ctataaaagc
     7621 aactggaaaa ggaattttcg ttccttatat catggctggt gaccacgaga aaggtttgga
     7681 cggtcttgct gaaacaattc actttttaga agattttggt gtctctgcaa ttgaagtggg
     7741 tattcccttt tcagaccctg ttgcagatgg acctgttatc gaagaagctg gcttgcgcag
     7801 tttagcccac gggacctcta cccaggcttt agttgaaacc ttgaaaacca ttgaaacaga
     7861 gattccactg gtcatcatga cctacttcaa ccccctcttt cagtacggtg tggagaactt
     7921 tgtcaaagat ctggctgata cagctgtgaa gggcttgatt atcccagact tgcctcatga
     7981 acatgccaac tttgtagaac catttttggc agatacagat atcgccttga ttcctttagt
     8041 aagcttgacc acaggaattg agcgccagaa agagttgatt gaaggggcgg agggattcat
     8101 ctatgccgtt gccatcaatg gagtgacagg gaaatcaggc aattaccgtg cagatttgga
     8161 caagcacttg gcgcaacttc atcaagtggc cgacatccca gtcttgacag gttttggtgt
     8221 atctagtcaa gccgatctgg aacgcttcaa tgcggtgtca gatggtgtta ttgtcggttc
     8281 gaaaatagta aaagctctcc accaaggaga gccgattcag gactttatca aacaagcagt
     8341 agcttaccaa aaataatcaa acaagtagca agtaagagga aaggcacgaa aggaagtctt
     8401 tcctttttct tttgcaggat aaaggccagt tttatagtga attgaatctg gaatattaca
     8461 ctacggttcc taaaatattt tcagaaagtg atttgaattt cccaatagat ttgttcagtt
     8521 tttaattgat tttactatag aagatataat tttctaaatt actctgacac agaactttaa
     8581 gaaaaagcta attattgtag taatttattt aagaaaatga tataattcat taaaaagtat
     8641 ttggaggagg aagaattatg ttgaatcagg atctctttga ttcgcttgag gcacaaaaaa
     8701 ttgtagatac tttgatgaaa gggcaaaaag attatgtaga tgaacgtcta gaaaaaagag
     8761 agacaatgat agtgtcgaat ggttatgcat ggacgcgacc taatcatatt gatactgcat
     8821 ttgcatcagc agatttgttt gagtataaat tacaattagc aggacagact tggggatatt
     8881 tagaatttga aacaaataca gaaaaatatg ggaaagtatt gttaattata aagggtaaga
     8941 agcgacttac gaaccaattt cctttggtac aaaaaaataa gagtggctac ttatttgaat
     9001 acgctcagat gaatacactt tatcttaatc aacattcttc ctacaaaaat gatgaagata
     9061 gtcattcctt tccaattcag atggagttag tttctgatga aatgattcaa gaaattgaac
     9121 aagctactaa aaattcgaat atcgaaaaat ttatgatttt aacttatgag gcggactcag
     9181 aaaacaatat tatatctgta gatgttgtta tgcctgatgc acgaactggt cagttacact
     9241 tgattcaaga tttgtctgag tatattcaat caagttcgta ccatttcgaa gaagctaaat
     9301 atcaagatat tcctaatttt tcagaattat cggaaacaga agattttgaa attattccaa
     9361 gaatagaaaa acaagaaggt caaaagtagt aaggagtatg taaatgttta atggtcgggt
     9421 attgaaagaa ttacggctgt taaatggttt aagtagagca gaattagctc agagaattaa
     9481 tttaacggaa caagccattt ggcagtttga gtccaacgaa acgaaaccta aattatcaac
     9541 caaaatgcat ttggccaacc aatttcatgt tgatttaact tattttgaac aggaagaaga
     9601 gagcgttcga tttgattctt ctgtaattgc ctttagaaat gcagacctag caacacggaa
     9661 aacaatagat attcaaacta tgtatttaca taaggtagat agtttgattg attattttga
     9721 aagttttgta attataccta atattataat tcatgaccta agtaatgtag tgagtgaatc
     9781 ttatcataag ggagaatcca ttgaggaatt ggctctttat gccagggaaa aattaggtat
     9841 ttcaaaagat aatcatgatt tgctttataa attagaacgt tcaggcatct atatcgtgga
     9901 acgattaatt aatggccaag ctgatgctta tagcgcatgg tcaaaattgg gaagacctta
     9961 tattgtgttg ggaacgaata aatcatctgt acgtcgaaat tttgacttag ctcatgagct
    10021 aggacatatt cttttacata aatataaaga tatgaatgaa gatggcaatc gtttggagca
    10081 agaagcaaat tattttgcat catgtttttt attgccaaaa gaagagtttt tagtcaaatt
    10141 tgaagagagg gttggcaagc gtgtcagcaa tcctgatagt tatattttat tgaagtcgga
    10201 tttgaatgtt tcgatacagg ctttagagta tcgagctttt aagttaggat tattgactcc
    10261 aaagcaacat tcttactttt atcgtcaaat tgcgcaaaaa ggttacaaaa tgattgaacc
    10321 tttggatgat caaatttttg ttaaaaaacc aagcaaagta aagagtattc tggacgtcgt
    10381 tttgagtaat catctagtca gtctagcgac tataatgtct aaacaaagta ttcgtttaca
    10441 gtttataagc gaaatatttt cagtcgaaat gaaatttttt gatcagtatc aagaagatag
    10501 aagaacagat cgatttgata acatcatccc tttgtacaaa agaaataatt tataatttaa
    10561 ctgtgaaata atttcatgaa ggacatatgg cagaaattgg attttatcag acaagcagta
    10621 gcttaccaaa aataatccca caagcagcaa gtaagaggaa aggcacgaaa ggaagtctct
    10681 cctttttctt ttgcaggaga aaggccagga tacccgtcgc agaagcaaac tgaatcaaga
    10741 tcagtaactc cgttacgcta aagacgagag cacaagaagc taaaaagagg aaatcccctg
    10801 cgcccatgcg gatatcgata aaatgagcca aaattccaag agcaaggaag gagaccatga
    10861 ccagattcca gccagaggaa gctattagga ttaggtggaa agtcatccag accagtaagg
    10921 gatattcctg atggtgaaag tcgtagatac ccaaggtcaa accagcggtg attaggacga
    10981 cttgccccaa ggaaagcaat tcccaagagt aaagcagaaa gaggagtcct aagactaatt
    11041 caaagagggc ataccagaca ggatagcgaa ctttgcagta gcgacagcga aagcgattga
    11101 agacctgtga gagaatcgga atcaaatcta agggacgcaa gcgagtctga caggaatcgc
    11161 agtgactggc tgaactgata atggattgct ctggaaaacg gtcaatgacc aaaccaagaa
    11221 aggaagcgag aatgctcccg acgagaaaaa aataaaaatc aatcatactt atctattcgt
    11281 aaaaaatggg agaaatagta taatggagaa acatagataa gatggtgagg aaaagatgac
    11341 aattcgtttt gaagaaaagg tgagtataga aaacgctcaa tttgtatgcc agtggtccaa
    11401 ctccctaggc aaatcctttc aagaacaatg gatgggacca aggattcctt ttctactgac
    11461 ccttcaagcc ttggaaggag tcttttcaat ctttgatgaa caagagtttg tgggacttat
    11521 ccagaaaatc aggctagaag acagcaatct tcatatcggg agatttttta tcaaccccca
    11581 gaaacaggag caagacttag gtagccaggc tttaaggaaa tttgttagtt tggcctttga
    11641 aaatgaagat atagatagta tttctctaaa tgtcttcgag gcaaatcaaa gagctcagaa
    11701 tctttaccaa aaagaaggat ttgaaatcgt tcaaatggtt gaagcacctg tac
//
LOCUS       DAVHEX010000063        11588 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVHEX010000063 DAVHEX010000000
VERSION     DAVHEX010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11588
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.063"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            124..423
                     /locus_tag="VVL45_001788"
                     /pseudo
     CDS             124..423
                     /locus_tag="VVL45_001788"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(701..1471)
                     /locus_tag="VVL45_001789"
     CDS             complement(701..1471)
                     /locus_tag="VVL45_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU4187926.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTIKRFSQSKEIKWRLIFTVSFSSVLGGFLGYLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(1486..2334)
                     /locus_tag="VVL45_001790"
     CDS             complement(1486..2334)
                     /locus_tag="VVL45_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEU4187927.1"
                     /translation="MKMKKIKGGTFMMGTNSEEGFLDDFEGPQVAVSVKDFSIADTPV
                     TNQEFAQFVKETGYKTLAERQEWSFVFILFVPEAEREGYPHPAGAPWWLQVPNACWKH
                     PYGENSNLVGLEDHPVVHVALEDALAFCNWSGMSLPTEAQWEYAARGGRQSEYPWGDT
                     LLEGGYYHANTWQGRFPYENTALDGFIGTAPVYEFLPNDFGLYQMIGNVWEWCRNPRY
                     TLLASFNEDDYELPKYGIQDEEYAIRGGSFLCHCSYCNRYRVAARNGCISTSTSSHLG
                     FRCLKE"
     gene            complement(2331..3806)
                     /locus_tag="VVL45_001791"
     CDS             complement(2331..3806)
                     /locus_tag="VVL45_001791"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEU4187928.1"
                     /translation="MVQTKQPNIILIVVDQMRADALSLNSKDKLVSTPTLDMMASVGY
                     NFENAYSPVPSCVPARAALLTGLDQDKSGRVGYQDEVPWNFTNTLPKVFKDMGYQTEC
                     IGKMHVFPSRQRLGFDHVLLHDGYLHVDRKYDKAYGSQFDYASDYLAFLKGKVGYDVD
                     LIDDGMDCNSWEARPWDKDEKLHPTNWVVSESISFLQRRDPTVPFFLKMSFEKPHAPL
                     NPPKYYFDMYMERLPQFLDLHIGNWEVLEKQIPSIYALRGKLKEDDQRRMVAAYFGLI
                     THIDHQISRFLTALKEFRHDKDTIIWFVSDHGDQLGEHYLFRKGYPYQGSIHIPSFIY
                     DPAGLIAGNRGTIKQLVKIQDIFPSLVDLAGGTTTDELDGRSVKNLLFGQYEGWRTEF
                     HGEHALGKDSSQYILTDQWKFIWFPVLNHYQLFDMKKDPHEMNDLYPSEKYQPIVRQM
                     KKKLVDFLRYREEGFVVDEELVPVELSKITPTLTKTGDSQS"
     gene            complement(4129..5484)
                     /locus_tag="VVL45_001792"
     CDS             complement(4129..5484)
                     /locus_tag="VVL45_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU4187929.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPG
                     FMAMYIVAILTYVFHLLSNDLLIDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFG
                     LHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGAFVWFGGLGLVLAILI
                     FSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATS
                     WGLVSPVTQNVTWVMPPILYGFFSTAFDWRAIILSVVCLIISVLTYFPFVKMAYKTEL
                     S"
     gene            complement(5494..5811)
                     /locus_tag="VVL45_001793"
     CDS             complement(5494..5811)
                     /locus_tag="VVL45_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU4187930.1"
                     /translation="MDESNLESVMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTREAQGEKTSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            complement(5828..6145)
                     /locus_tag="VVL45_001794"
     CDS             complement(5828..6145)
                     /locus_tag="VVL45_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU4187931.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            complement(6158..7843)
                     /locus_tag="VVL45_001795"
     CDS             complement(6158..7843)
                     /locus_tag="VVL45_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HEU4187932.1"
                     /translation="MKNKKLILSLFILFLLIFIHAKVFANENLIKNSNFEQGELFWNS
                     RNRGIINSNESYSNGHYSGMIPSTAINDNRADGYIGQVIDIEPNEDYLVSAFAKVDIS
                     GAEGYFTARWFDNNKQGEIVQNNIGEAVDQTVNTTEWKKYMFSFNSGKHDKVLIQLVK
                     WSEDDTTKKSNIFIDNVEMYQLSKGNSYKKIWRDDFDGEQLNKKYWGYELGSIRGWEQ
                     QHYVRSDENVFLRSGNLVLRATNRSKEDQYFNPRNNHRKVVYNSGSVRTHGKVEFLYG
                     KLEMRAKLPKGQGVFPAFWTLGSDFTLDGKINPVQGRGWPSTGEIDIMELVGERNSNG
                     SGNKTVYQTLHYGQSDNDDGKFAGRGTAFKLSSGNFNDSYHTFSIDWYKDYIVWMVDN
                     HIVRKVYYGSDNIAKKIFNRPQYVQLNLAMGGSWPGVVDNNLSGTEFVVDYVSYSRND
                     LQQHQAEEYYSQSPKINGAKDIVINRGDVPDLLKGITTNSGYELDYSIENEPMFNNFG
                     GNTSVDLLIRNKTEKEKISQMPPGVYNLHYTSRPKHLDYESKVDRKTVTLTIK"
     gene            complement(7848..8744)
                     /locus_tag="VVL45_001796"
     CDS             complement(7848..8744)
                     /locus_tag="VVL45_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEU4187933.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLKRIEELGFDFVCLTGNPGTGVSNREIIKAVQTAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHGKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            complement(8747..10009)
                     /locus_tag="VVL45_001797"
     CDS             complement(8747..10009)
                     /locus_tag="VVL45_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU4187934.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFITGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQVALGGPLGLLGNNV
                     IGLLILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFM
                     DNFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDPGWTMPPVISGLLATGSISGSLL
                     QIVLIVLDVLLYLPFVIAIEKRFKLLED"
     gene            10289..11467
                     /locus_tag="VVL45_001798"
     CDS             10289..11467
                     /locus_tag="VVL45_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEU4187935.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLINQTLKQFLNNCSDYEIEAIGIALPGRYLDNYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
BASE COUNT         3982 a         2420 c         1606 g         3580 t
ORIGIN      
        1 gtacaggtgc ttcaatagtt tctatagtaa aatgaaccaa aaatagtaca cgttgcggta
       61 taatgttctt gtggcatatt cattagattt tcgtaaaaag ttctctccta tttgtgagcg
      121 aacaggtata gtcaattgat tgtcagtttg aagaccaaga gtcgtaaggc aaaggatatg
      181 gcagaatcca tccaagggtg gctggcacaa tttctagtga acctctttaa atctattacg
      241 tttgactgtg gaaaagagtt ttcaaaatgg aaagatattt ccaatcatca cgatagtgag
      301 agcttttttg cgaatctagg atgtccaaga cagcggtgtc tcaacgaaca ttcaaataga
      361 cttttaaggt gtcatgactt gccaaagcag acagatttca acgaagtcag tcaagaattt
      421 taggctgggt aaaaactcaa tttcaggaga aaatgaaata aatattccca caataaaacg
      481 catagtatca aagtttttcc aacacctgat attatacatt ttttaatttt aaagactttt
      541 tcccagcctc aagaaatcac atcccaccta aatcctttgg ataccaaata cctcatcagg
      601 tattttctga gttggtaaga ctgtctagct taatttgaca aatggagggc gttaaatcca
      661 gtttttacag gaagtctatt taaatatttt cacactgacc ttacaaaata tgaaataaat
      721 tatataaagt cattcccatg acaaagaata acatcccttt aaaacagtat cttacccaat
      781 tttcaggtaa gacttttgat acaaccgtcc ccaatacccc tccgcatata gaagcaagaa
      841 aaataactgg aacaggtgct aaatgatact gatttaaacc tgtaaccaca acaattgtag
      901 ccaaatgaga cagctgggaa aagaaaataa ttgcaagaga atacatcgtt gcttctttta
      961 tcgaaataga aaaaaacacc atcaataaag atacattgag cggaccacca ccaatcccta
     1021 aaaaactaga aatcaagccc aatagaagac cacaaatcat atagaatcct atcttatcaa
     1081 attgataagt ttttttaaaa tctgttaaaa caaatgatac aagtaacatc acaaatagta
     1141 aaatcatttg aacaatagat actagccgta ctgataattg agataacaaa acttgaaaaa
     1201 taagataacc tagaaatcct cctaaaacag aagaaaagga taccgtaaaa atcaatctcc
     1261 attttatctc tttactttgt gaaaatcttt tgattgttga aataatcgcc attataaaaa
     1321 cagagaaact agaataaaaa gcaatatcac tgaccgaatg gtatccaaaa ctatccataa
     1381 gtggcttaat tataattcca ccacccatgc ctgatataga acccaatcca ctagctatta
     1441 taatgattag aaaataaata atctcaacca ctttttaagt ccatcctatt ctttcaaaca
     1501 tctaaaacct agatggctac tagtggaagt agaaatacat ccatttctag cagctactcg
     1561 gtatcgatta caataagaac aatgacataa aaaggaacct cctctgatag catactcctc
     1621 atcttgtatc ccatattttg gtaactcata gtcatcttca ttaaaactag ctaataaggt
     1681 atatctcgga ttgcgacacc attcccaaac attcccaatc atttgatata gaccaaaatc
     1741 attaggtaaa aattcataga caggagccgt tcctataaat ccatctaagg cagtattttc
     1801 ataaggaaat cttccttgcc aagtattggc atgataatat ccaccttcta aaagagtatc
     1861 tccccaagga tattcagatt gtcttcctcc tctagcagca tattcccact gtgcttcagt
     1921 cggtaaagac attccgctcc aattacagaa agccaatgca tcttctaaag caacatgaac
     1981 tactggatgg tcttctaaac ccactagatt actattctca ccgtagggat gtttccagca
     2041 agcgttcgga acttgtaacc accacggagc cccagcagga tgaggatatc cctccctttc
     2101 tgcctctgga acgaacaata taaaaacaaa agaccactct tgtcgctcag ctagtgtttt
     2161 atagcctgtt tctttcacaa attgagcaaa ttcttgattc gtaactggag tatccgcaat
     2221 ggaaaaatcc tttacactaa cagcgacttg aggaccttca aaatcatcta aaaagccctc
     2281 ttcagaattt gttcccatca taaatgtccc acccttgatt tttttcattt tcatgattga
     2341 ctatcccccg ttttcgttag agttggcgtt attttagaaa gttcaacagg aactaactct
     2401 tcatccacaa caaaaccttc ttctctatat ctaagaaagt ctactaattt tttcttcatc
     2461 tgacgaacaa taggttggta tttttcactt ggatacaaat cattcatttc atgcggatct
     2521 tttttcatat caaataactg ataatgattt aaaacaggaa accagataaa tttccactgg
     2581 tctgtcaaga tatattgact agaatctttt cctaaagcat gttcaccatg aaattctgtt
     2641 cgccacccct cgtactgtcc aaataataaa ttcttaacac tccgtccatc tagttcatct
     2701 gtagtagttc ctcctgccaa atccacgagt gaaggaaaaa tatcttgaat tttcactaat
     2761 tgcttgattg ttcccctatt accagctatc aatccagccg gatcgtaaat aaacgaagga
     2821 atgtggatac ttccctgata aggatatccc tttctaaaca gataatgttc accaagctga
     2881 tctccatgat ccgaaacaaa ccagataatc gtatctttat catgacgaaa ttcttttagg
     2941 gcagtcaaaa agcgactaat ttgatggtct atatgagtaa tcaatccaaa ataagcagca
     3001 accattcttc tttgatcatc ttcctttaat ttccctctta aagcataaat agacggaatt
     3061 tgcttttcta atacttccca attcccgata tgcaaatcga gaaattgagg caagcgttcc
     3121 atatacatat caaaatagta ttttggggga tttaatggag catgtggttt ttcaaaagac
     3181 attttgagaa aaaaaggaac cgttggatct cttctttgta aaaatgaaat ggactcactc
     3241 acaacccagt ttgttggatg taacttctca tctttatccc aaggtctagc ttcccaagaa
     3301 ttacaatcca ttccgtcatc aatcaaatca acatcataac caactttacc ctttaaaaag
     3361 gctagatagt cactagcata atcaaattga cttccataag ccttatcata ctttctatca
     3421 acatgtagat acccatcgtg aagtaagaca tgatcaaacc caagtctctg tctggaagga
     3481 aaaacatgca tcttaccaat acactctgtt tgataaccca tatctttaaa gacttttgga
     3541 agagtattag taaaattcca aggcacctca tcttgataac caactcgtcc acttttatct
     3601 tggtctaagc cagtcaataa agctgcccta gcaggaacgc aagaaggaac tggagaatag
     3661 gcattttcaa aattataacc tacactagcc atcatatcta atgttggagt gctaactagc
     3721 ttatccttac tattcaagga taaggcgtct gctctcattt gatctacaac aatcaaaata
     3781 atatttggct gttttgtctg aaccataaaa tctcctttct aatatggcaa aagaggcaca
     3841 agaagatatc tacctttact gcaccccttt ctatatcaat ctctctatat aaaacaataa
     3901 cattcttgtt atgttttata gaaaaatgaa ctaaaatatg actaaatcga ttaggaaatt
     3961 caaatcattt tctagtactg ttttagaagt tacagtgtac tattccaact tcaataaact
     4021 ataaaccttt gtctaataac aattttagtg gagataagaa atcctacacc taactcatct
     4081 tacacgtaat ctatttctat tttatcacaa aaaacgcaag taagaccatt aactcaattc
     4141 agttttatat gccattttca caaatgggaa ataagtcaag acactaataa tcaaacaaac
     4201 aactgataag atgatggcac gccaatcaaa tgctgtagag aagaaaccat ataaaattgg
     4261 aggcattacc caagtaacgt tttgtgtaac aggtgaaaca agaccccagc ttgttgccca
     4321 gtaagctacc gttgccatga aaaccgggct aagtacaaat ggtataaata gcaaaggatt
     4381 caagacaact ggtaaaccat aattgaccgg ctcaccaata ttaaacagaa ctggtgctag
     4441 accaagttta gcaacttttc gataatgact gtttcttgaa aaaattaaaa tagcaagtac
     4501 taatcctaat cctccaaacc agacaaacgc cccaaaagac ccacttgtcc atatataagg
     4561 aatcggttca cctttttgga aagcatccag attcgctaac atagcaactc caaacagccc
     4621 ttccatgatg ggagccaata catttcctcc atggagacca aaaaaccaga ataacttatt
     4681 caaaaagatc atcagaataa ctgcaaagaa actttgagac aaacctagta atggcgtttg
     4741 taacaccttg taaacccaat caatcaataa gtcattgcta agtaaatgga aaacataagt
     4801 caagatggct actatataca tcgccataaa tcctggaatg atagaagtga acggcttagc
     4861 aatcgcaggg ggaactgaat ctggtaactt gattacccag ttctttttca ttactttaca
     4921 gaaaataata gaggctaaaa atccaatcat catggctgta aagtagcctc tggcattaat
     4981 atggtttcct ggaatcacat tcccaatagt taccatcaga tttttaccat caaatgctag
     5041 attatcaatt ccttgtaaga tttgatctaa tttcacatct cctacatttg ccaaagggaa
     5101 actctttgta actgtacttc caatcgaaat gacaaacgaa gcaagtgata ccaaaccagc
     5161 agaaactgta tcaaccttgt aaatcttagc gatattcact cccaagcaat agatgaacaa
     5221 caaggaaaca attggtatac ttcccttgaa taccaaatta ttgatgtcaa caagccactg
     5281 aaaggttttc gtaatacttt ctaggtgaaa ttgttgtggt aaatccacta gaaaagcatt
     5341 taataacaaa gcaatggaac ctgtcataat aacaggcata gtccccacaa atgaatcacg
     5401 taaagcaact aaaaacttct gattcccaac tttcattgca attggagaaa gtttttcttg
     5461 cattttatcc aacattgtat tcataccaaa ctcctaattt ttttcaaatc gttcgtatac
     5521 ttctaccact tctttcgcca agtcaacaaa agtcagactc gtcattaaat gatcttgcgc
     5581 gtgtaccatc aagaggctaa ttgatgtttt ttccccctgt gcctctcttg tcaacatttc
     5641 tgtttgagcc ttatgcgcct gtaataaggc agcattcgca tcagctaatc ttcgattggc
     5701 tttagagaaa tccccttttt ttgctgcctg aatagcctcc atagcattac ttttggcttc
     5761 cccaccatac ataattagcc ccattacaga ttctaaatta ctttcatcca taacattctc
     5821 ctcaatctta gtctaataaa ctttcagcta aatctaaaac tttagaacca ttcatcattc
     5881 cgtaatctgt catcggaata accgccacag gaatctgtct atcttttagt ttttcttgaa
     5941 agtcccctag taaatagcga acttgaggac ctaaaagcaa cacatttact tcttttgttg
     6001 ctacaatttc ttctgcttca ggagctggaa ctgcaaaaat ttctgcatcc aaccccttat
     6061 cttctgctgc cttttgcatc tttgtcacta gcagacttgt actcatacct gctgcacatg
     6121 ctaacataat tgttacttta gccatagctt actcctttta ttttattgtc aatgttactg
     6181 tttttcgatc aactttagac tcataatcta aatgtttggg acgactagta taatgcaaat
     6241 tataaactcc tggaggcatt tgtgaaatct tttctttttc agttttattg cgaattaata
     6301 aatctaccga agtattccct ccaaagttat taaacatagg ttcattttca atagaataat
     6361 ctaactcata tccactatta gttgtaattc cttttaataa atcaggaaca tctcctctgt
     6421 ttattactat atccttagcg ccatttattt tgggagattg actataatac tcttcagctt
     6481 gatgttgttg taaatcattt ctagagtaag aaacataatc aactacaaat tcagtccctg
     6541 ataaattatt gtccactacc ccaggccaac ttcctcccat cgctaaattt aactgaacat
     6601 actgaggtct attaaaaatc tttttcgcta tattatctga tccataatat acttttctaa
     6661 caatatgatt gtcaaccatc catacaatat aatctttata ccaatcaatt gaaaaagtat
     6721 gatacgaatc attaaagttt ccactcgata atttaaaggc tgtaccacgt cctgcaaatt
     6781 ttccatcatc attatccgac tgtccataat gaagcgtttg ataaactgtc ttattcccac
     6841 ttccatttga atttcgttca ccaactaatt ccattatatc tatctcacct gttgacggcc
     6901 atcctcgtcc ctgaacagga ttaatcttcc catcaagtgt aaaatccgat ccaagagtcc
     6961 agaaagcagg aaaaactcct tgacctttcg gtaattttgc acgcatctct aatttaccat
     7021 aaagaaattc tactttccca tgtgttctaa cacttcctga attatataca acctttctgt
     7081 gattgtttct tgggttaaaa tactgatctt ccttagagcg attcgtagct ctaagaacta
     7141 aatttcccga acgtagaaaa acattctcat cacttcttac ataatgttgc tgttcccaac
     7201 cacgaattga tcctaattca tatccccaat attttttatt taattgttcg ccatcaaaat
     7261 catctctcca aattttctta tatgaatttc ctttagacag ttgatacatc tctacattat
     7321 caataaaaat attagatttt tttgtagtat cgtcttctga ccatttcact aattgaatca
     7381 aaactttatc gtgtttaccg gaattgaaag aaaacatgta ttttttccat tcggttgtat
     7441 taactgtttg atcaacagct tctccaatgt tattttgaac aatctctcct tgcttattat
     7501 tatcaaacca tcttgcggta aaatatccct ctgctccact aatatctact ttggcaaaag
     7561 cagatactaa ataatcttca ttaggttcta tatcaataac ttgtccaata tatccatctg
     7621 ctctattatc attaattgca gtagaaggaa tcatacctga gtaatgtccg ttactataac
     7681 tctcatttga attaattatt cccctatttc tagagttcca aaacagctct ccttgctcaa
     7741 aatttgaatt ttttattaaa ttttcattag caaaaacttt cgcatgaata aatattaata
     7801 aaaataatat aaataaactt aaaattaatt tcttattctt cattttatca cctatttact
     7861 gacctagcca agcgagatac tgtatgtctc actcctctaa ttgccttgct caatgcataa
     7921 atattatcga ctgttgccag tcccccatat cctgcgtcac ctatatgttg aatatcaact
     7981 ccacaaattt tatttctaag tgcaatttcc ttgatagtat ctgtatcaga tgtttcttgg
     8041 ctagtaccaa tagcactcaa tacaagacca cccttaccat gaacgagatc aacgacttca
     8101 cgcaactctt ggtcatgaaa ggctggaacg gttccaactg ctggaacaag tatcacatcc
     8161 gcacctgctt ccaacaattg ctctgcgaca gaaagctctg ccacaggctc attcactcct
     8221 gccccgtgca tcttacctgc aataatcaaa ccagaaaagt tttccttagc agtttgaaca
     8281 gccttaatga tttctcgatt gctaactcct gttccaggat ttccagtcaa acagacaaag
     8341 tcaaaaccta actcctctat tcgcttcaat gtttcaacac tagcaacacg acccgcaaca
     8401 atttcctgtg tttcctctag catctttgca gatgggtcaa tcggttccaa atttacacca
     8461 attggacaag caacaagctg gtgtaagcga tgaataacat catcacctga actatccaaa
     8521 gcataaattt taggctcaaa aacatcaaca caattcaata aaatcatatc agctccaaaa
     8581 gctctagcaa tttcagaatt agttatatcc ccaacaaatg tctcacgagt aactacattt
     8641 tcagatagaa caactctacc ttcacttgct aaaatacttt gttttaattc ttcagcattc
     8701 atctgtaata tctcagatgg atttgcacta attaaacgtt tcattctcaa tcctccaata
     8761 acttaaaccg tttttcaata gctattacaa atggtaaata aagtaagaca tccaaaacaa
     8821 tcaatacaat ttgtaataat gaaccagaaa tacttcctgt agcaagtaaa ccacttataa
     8881 caggtggcat agtccaacct ggatctgtat aagttagcgg aaccaaacct gatgccattg
     8941 ctccccacgc tactagtaag ttaaacattg gagctaagat aaatggaact agtaacaaaa
     9001 tgttcaaaac aataggaacg ccaaacattg ctggttcatt tatattaaat aatccaggta
     9061 taacagttat aggtgccaaa gtttttagtt gtttactagc tttcttctta tgtgctaaat
     9121 atcctaacgc aagaactaaa cctatggttg caccaccgcc acctatgaat acaaaattat
     9181 ccataaatac gctagtaaaa atatttggaa gagtttctcc agtttgataa gctactttat
     9241 ttgcatctag attagctaac cataacggtt tcataactgc attaacaaca tttcctccgt
     9301 gtaatcctac aaaccaaaaa ctactattaa ggaaaattaa gataagaaga ccaataacat
     9361 tatttcctaa aagtccaagt ggacctccca aagctacttg agcaatttca tgaagattag
     9421 gtaaacttaa tttatctaaa gttgcataaa caatcaacca accaacaata gtcactgcac
     9481 caggaataat ggcactaaag cttttagata ctgctggtgg aacaccatcc ggcattttta
     9541 tctgtatatt atgatttata aaccattggt agatatagcc attaatcaat cctaaaatca
     9601 tagcaacaaa taaacctttt gatgccatga aagcggtagg cattcctgct ccagcctctc
     9661 ctgtaatgaa aggcgtaact gtaataaaag atgataatga cacaatacca gcaggaatac
     9721 catcaacttt gtattgtcta gccataaagt aagcgatacc aaaactagct acaagaccaa
     9781 gtaatccaaa actactatca acacccttcc ataaatagtc agcaacacca atatcaccca
     9841 gatatttttc ccatcctgga ataggaaaac ttgcaataac cattagtaat gaacctatca
     9901 acaataaagg tatagttaga gtaataccgt ctcgaataga aaccaacgct ttattattac
     9961 ccaatctagc cgctactgga agtaactttt cttgtaataa tttttccata accttttcct
    10021 cttaaatata ttttattatc aaaagttatc aattatcttt attttttgac gtcaagttgt
    10081 tatttgatta tcttttgtta tttatatttt acaactataa gaaagccttg tcaaggattt
    10141 gtgtaaacat ttgattattt tttattatca atatataata aaaagagacc agtaagtaat
    10201 gaaaagctta aaaagattaa catctacatt tatccatagc aaaaaatcat aggattttaa
    10261 gaatctgaca actaggaaga aaactaatat gaccttatct aagaaacaat tacaactaag
    10321 agctaaaata ctggaaacag tttacacatt aggtcccatt tcacgaattg aaatcgctac
    10381 taaaacagga ataacacctg caacaactag cagtatcaca aatgatctga ttaaagaaaa
    10441 tattctctta gaacttggtg aagacgaaca cgatacaagc gtagggagaa aaaaaatcct
    10501 gctagacatt caagctaaac gattttacta cattggttgt gaactatctg aaaaacattt
    10561 cacttttgct ttaggagata atctaggaaa tatcttaaag gaagagaaag aaattgttac
    10621 taaacagtta atccaagaaa agggaaatca actcattaac cagacactaa agcaattctt
    10681 aaataactgc tctgattatg aaatagaagc aattggaatc gcgctacctg gtcgctattt
    10741 agataattat aaaataacaa caaacaatcc tttatggcaa catatcgact tagaaatgat
    10801 tcaatcccac tttgataagc ctttgttttt ctctaacaat gttaactgta tggctatagg
    10861 taaacgccta tttagtcgcc aacagaatga ccctaatttt gcttattttc attttgctag
    10921 aggtatgcac tgttcttata tctacgacgg aaacatttat gggaaaggaa atctaatgat
    10981 tggagaaata gggcatacag ttgtttcatc tgagggagaa gaatgtagct gcgggcgcaa
    11041 gggatgctta caaacttttg ctggagaatc ttggttaatc aaaaaatcaa aaattctata
    11101 tcatcaatct ccctattctc tacttcctag tttagtcaaa aatgccgatg atattgatat
    11161 ccaagttatt ttaactgctt atcaattagg tgacactgga ataatcacac ttatccatca
    11221 agccttactc tatcttagtc aaaccatttt aaatatcagt atgatgattg actctcaaaa
    11281 aatctaccta catagtcctt tactaacaaa tcaacacatc atccaaaaac tatatagtga
    11341 aatgaactac aaacccaaac tgctctataa tcgcctacct gaagtcatta ttgaacctta
    11401 caatgatttt acagccgcac actcagctat tgcactttgt ttgtatcata caattctaca
    11461 ttcttaattt tctattacat ttaataaata ggctaaaaag agggagtata tcataaaaag
    11521 cgaataaacc tgttttctga aaatcagaat tctgttttgt tcgctttctt tatatttgga
    11581 attatact
//
LOCUS       DAVHEX010000064        11515 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVHEX010000064 DAVHEX010000000
VERSION     DAVHEX010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11515
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.064"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            168..1439
                     /locus_tag="VVL45_001799"
     CDS             168..1439
                     /locus_tag="VVL45_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU4187936.1"
                     /translation="MSSKFMKSAAVLGTATLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIVGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            1550..2842
                     /locus_tag="VVL45_001800"
     CDS             1550..2842
                     /locus_tag="VVL45_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU4187937.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            2844..3686
                     /locus_tag="VVL45_001801"
     CDS             2844..3686
                     /locus_tag="VVL45_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU4187938.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            3939..4739
                     /locus_tag="VVL45_001802"
     CDS             3939..4739
                     /locus_tag="VVL45_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEU4187939.1"
                     /translation="MLPYPFSYFSSIWGVRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTDKVVQDLSEHATIVDGTLTYTGTASQAPSVVIG
                     PSQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASFLLYITKRSRLFSFNTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            4749..5735
                     /locus_tag="VVL45_001803"
     CDS             4749..5735
                     /locus_tag="VVL45_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU4187940.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            5871..5951
                     /locus_tag="VVL45_001804"
                     /pseudo
     CDS             5871..5951
                     /locus_tag="VVL45_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(6161..7102)
                     /locus_tag="VVL45_001805"
     CDS             complement(6161..7102)
                     /locus_tag="VVL45_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEU4187941.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(7080..8843)
                     /gene="aspS"
                     /locus_tag="VVL45_001806"
     CDS             complement(7080..8843)
                     /gene="aspS"
                     /locus_tag="VVL45_001806"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEU4187942.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEQEIQDITESLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVRGVDFKVFSEAPAVKAIVVTGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALVLEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSTEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
     gene            complement(9079..9159)
                     /locus_tag="VVL45_001807"
                     /pseudo
     CDS             complement(9079..9159)
                     /locus_tag="VVL45_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(9195..9425)
                     /locus_tag="VVL45_001808"
     CDS             complement(9195..9425)
                     /locus_tag="VVL45_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187943.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            complement(9443..10084)
                     /locus_tag="VVL45_001809"
     CDS             complement(9443..10084)
                     /locus_tag="VVL45_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU4187944.1"
                     /translation="MKLLKNLGWILLALLSFLFIYGFIQGLATASLALGASPYAVTLL
                     YVALAGVYVYGIYKWYQKAPVHIEKSGFNRFIWLPVLVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            complement(10096..10776)
                     /locus_tag="VVL45_001810"
     CDS             complement(10096..10776)
                     /locus_tag="VVL45_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEU4187945.1"
                     /translation="MGMFVGMFKARVESHEIILDVKALMPWISAICLLIGFISMFLTF
                     NFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAFNITGVAIPLAIFISLSEV
                     IILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLEFFATPKDVLNYINSYDEG
                     ERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQLLSFLLVGAIHVYINVMQL
                     PMVKRYFK"
     gene            complement(10832..11029)
                     /locus_tag="VVL45_001811"
     CDS             complement(10832..11029)
                     /locus_tag="VVL45_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187946.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVIEWIEKKSKNLASEKGE"
     gene            complement(11029..11235)
                     /locus_tag="VVL45_001812"
     CDS             complement(11029..11235)
                     /locus_tag="VVL45_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU4187947.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            complement(11245..>11515)
                     /locus_tag="VVL45_001813"
     CDS             complement(11245..>11515)
                     /locus_tag="VVL45_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4187948.1"
                     /translation="NNSKKIKKLENKIKRLERKEKGNAEMSRLLQEMIGKEPIITGVY
                     IGPDNWEVVDVDEEWVKLRRVDNTGKEKFKLQRIEDIQTVEFDGE"
BASE COUNT         3473 a         2564 c         2110 g         3368 t
ORIGIN      
        1 tctattaaaa tgcaaaatta tgctatcaaa tcaagcttaa aaatttttta aaatttttac
       61 aaaaaatact tgcaaccgtt ttctatttgt gctatactaa gctcataaag gaaaacgttt
      121 gcgtttcctt atcaataaaa cttgctattc tttaggagga atacactatg tcatctaaat
      181 ttatgaagag cgctgcggtg cttggaactg ctacacttgc tagcttgctt ttggtagctt
      241 gcggaagcaa aactgctgat aagcctgctg attctggttc atctgaagtc aaagaactca
      301 ctgtatatgt agacgaggga tataagagct atattgaaga ggttgctaaa gcttatgaaa
      361 aagaagctgg agtaaaagtc actcttaaaa ctggtgatgc tctaggaggt cttgataaac
      421 tttctcttga caaccaatct ggtaatgtcc ctgatgttat gatggctcca tacgaccgtg
      481 taggtagcct tggttctgac ggacaacttt cagaagtgaa attgagcgat ggtgctaaaa
      541 cagacgacac aactaaatct cttgtaacag ctgctaatgg taaagtttac ggtgctcctg
      601 ccgttatcga gtcacttgtt atgtactaca acaaagactt ggtgaaagat gctccaaaaa
      661 catttgctga cttggaaaac cttgctaaag atagcaaata cgcattcgct ggtgaagatg
      721 gtaaaactac tgccttccta gctgactgga caaacttcta ctatacatat ggacttcttg
      781 ccggtaacgg tgcttacgtc tttggccaaa acggtaaaga cgctaaagac atcggtcttg
      841 caaacgacgg ttctatcgta ggtatcaact acgctaaatc ttggtacgaa aaatggccta
      901 aaggtatgca agatacagaa ggtgctggaa acttaatcca aactcaattc caagaaggta
      961 aaacagctgc tatcatcgac ggaccttgga aagctcaagc ctttaaagat gctaaagtaa
     1021 actacggagt tgcaactatc ccaactcttc caaatggaaa agaatatgct gcattcggtg
     1081 gtggtaaagc ttgggtcatt cctcaagccg ttaagaacct tgaagcttct caaaaatttg
     1141 tagacttcct tgttgcaact gaacaacaaa aagtattata tgataagact aacgaaatcc
     1201 cagctaatac tgaggctcgt tcatacgctg aaggtaaaaa cgatgagttg acaacagctg
     1261 ttatcaaaca gttcaagaac actcaaccac tgccaaacat ctctcaaatg tctgcagttt
     1321 gggatccagc gaaaaatatg ctctttgatg ctgtaagtgg tcaaaaagat gctaaaacag
     1381 ctgctaacga tgctgtaaca ttgatcaaag aaacaatcaa acaaaaattt ggtgaataaa
     1441 aaatttgttc aaggggggtg gaaatcaaat ccccctttga atttatcaat agagacacaa
     1501 ataatttagc tttcttataa aaaagtagta tcctatgaaa ggagttaata tggaaaagca
     1561 acaacctagt aaagcagccc tgctgtctat cattcctggg ttaggacaga tttacaataa
     1621 acaaaaagcc aaaggtttta tcttccttgg tgtaaccatc gtatttgtcc tttacttcct
     1681 agcacttgca acccctgaat tgagcaacct catcactctt ggtgacaaac caggtcgtga
     1741 taattccctc tttatgctga ttcgtggtgc cttccatcta atctttgtaa tcgtttatgt
     1801 actcttttat ttctcaaata tcaaagatgc acatacgatt gcaaaacgca ttaacaatgg
     1861 aattccagtt ccacgcacac tcaaagacat gatcaaaggg atttatgaaa atggcttccc
     1921 ttacctcttg atcattccat cttatgttgc catgaccttc gcgattatct tcccagttat
     1981 cgtaaccttg atgatcgcct ttaccaacta cgacttccaa cacttgccac caaacaagtt
     2041 gttggactgg gttggtttga ccaactttac aaacatttgg agcttgagta ccttccgttc
     2101 tgcctttggt tctgttcttt cttggactat catttgggct ttggcagctt ctactttaca
     2161 aatcgtaatt ggtatcttca cagctatcat tgccaaccaa ccatttatca aaggaaaacg
     2221 tatctttggt gttattttcc ttcttccttg ggctgtccca gccttcatca ctatcttgac
     2281 attctcaaac atgtttaacg atagtgtcgg tgctatcaac actcaagtat tgccaatctt
     2341 ggctaaattc cttcctttcc ttgatggagc tcttattcct tggaaaacag acccaacttg
     2401 gactaagatt gccttgatta tgatgcaagg ttggctcgga ttcccataca tctacgttct
     2461 gaccttgggt atcttgcaat ctattcctaa cgacctttac gaagcagctt atattgacgg
     2521 tgccaacgct tggcaaaaat tccgcaacat cactttccca atgattttgg ctgttgcggc
     2581 acctactttg attagccaat acaccttcaa ctttaacaac ttctctatca tgtacctctt
     2641 caatggtgga ggacctggta gtgtcggagg tggagctggt tcaaccgata tcttgatctc
     2701 atggatctac cgtttgacaa caggtacatc tcctcaatac tcaatggcgg cagctgttac
     2761 cttgattatc tctatcattg tcatctcaat ctctatgatc gcattcaaga aactacacgc
     2821 atttgatatg gaggacgtct aagatgaata actcaattaa actcaaacgt agactgactc
     2881 aaagccttac ttacctttac ctgattggtc tatcaattgt aattatctat ccactgttga
     2941 ttaccattat gtcagccttt aaagcaggta acgtctcagc ctttaaacta gatactaata
     3001 tcgacctcaa ttttgataac tttaaaggcc tcttcactga aaccttgtac ggtacttggt
     3061 acctcaacac tttgattatc gccttaatta ccatggctgt tcaaacaagt atcatcgtac
     3121 ttgctggtta tgcttacagc cgttacaact tcttggctcg taaacaaagt ttggtcttct
     3181 tcttgatcat ccaaatggtg ccaactatgg ccgctttgac agccttcttc gttatggcgc
     3241 ttatgttgaa cgcccttaac cacaactggt tcctcatctt cctctacgtt ggtggtggta
     3301 tcccgatgaa tgcttggctc atgaaaggct acttcgatac agtgccaatg tctttagacg
     3361 aatctgcaaa actagacggt gcaggacact tccgccgctt ctggcaaatt gttctaccac
     3421 ttgttcgccc aatggttgcc gtacaagctc tctgggcctt catgggacct ttcggggact
     3481 acatcctctc tagtttcttg cttcgtgaga aagaatactt tactgttgcc gtaggtctcc
     3541 aaaccttcgt taacaatgcg aaaaacttga agattgccta cttctcagca ggtgctatcc
     3601 tcatcgccct tccaatctgt attctcttct tcttcctaca aaagaacttt gtttcaggac
     3661 ttacaagtgg tggcgacaag ggataattta tccccgccac cctttttcat tttatactct
     3721 tcgaaaatct cttcaaacca cgtcagcttt atctccaacc tcaaagttgt gctttgagca
     3781 acctgtggct agtttgcact ttgattttca ttgattatta gcaattgtca ctgtaaataa
     3841 tatccttgta gcaagcaatt tttctcctag acttgaaata aagcgcattt ctctatataa
     3901 taatactcat atagaaaaca ccttttagaa agatacctat gcttccatat ccattttcct
     3961 atttttcaag tatttggggg gttcgtaagc ccctgtccaa acgtttcgag ctcaactggt
     4021 ttcaacttct ctttaccagt atcttcctta tcagcttgtc tatggtaccc attgctatcc
     4081 aaaacagctc ccaggagacc tatccgctag aaacttttat cgataatgtc tatgaacctc
     4141 tgacagataa ggttgtccag gatctctctg aacatgctac aattgtcgat ggcacattaa
     4201 cttatactgg aacagctagt caagcccctt ctgttgtgat tggtccaagt caaatcaagg
     4261 aattacctaa ggacttgcaa ctgcatttcg atacaaatga gctagtcatc agcaaggaaa
     4321 gcaaggaact gacccgcatc tcttaccgag ccattcagac tgagagtttc aaaagcaaag
     4381 acagcttgac ccaagcaatt tctaaagact ggtaccaaca aaatcgtgtc tatatcagcc
     4441 tcttcctagt tctcggtgcg agcttcctct ttggtttgaa tttctttatc gtctctcttg
     4501 gagctagctt tctcctttat atcaccaaaa gatcacgcct cttttcattt aataccttta
     4561 aagagtgcta ccattttatc ttgaactgtt taggattgcc gactctgatt acacttattt
     4621 tgggattatt tggccaaaat atgacaaccc tgattactgt acaaaatatt ctttttgttc
     4681 tgtatctggt cactatcttt tataaaacac atttccgtga tccaaattac cataaatagg
     4741 agatttttat gcccgttacg attaaagacg tggccaaggc tgctggtgtt tcgccttcaa
     4801 ccgtaacccg tgttattcaa aataaatcaa ccattagcga cgaaacaaaa aaacgtgttc
     4861 gcaaagctat gaaggaactc aactaccacc caaacctcaa cgctcgtagc ttggtaagca
     4921 gctatactca ggttatcgga ttagttcttc ctgatgactc agacgccttc taccagaatc
     4981 ctttctttcc atcggttcta cgtggcatct ctcaagtcgc atctgaaaac cactatgcca
     5041 ttcagatagc aacagggaaa gatgagaagg agcgtctcaa cgctatttca caaatggtct
     5101 acggcaagcg tgtagatggg ctaatttttc tctatgccca agaagaagac cctctcgtaa
     5161 aactcgtcgc agaagaacag ttccccttcc ttatcttagg taaatctcta tctcctttca
     5221 tcccacttgt cgacaacgac aatgttcaag ctggttttga tgcgactgaa tatttcatca
     5281 aaaaaggctg caaacgcatt gcctttatcg gaggaagtaa aaagctcttc gtgaccaaag
     5341 accgtttaac aggctatgaa caggcgctta aacattacaa acttaccact gacaacaatc
     5401 gcatctactt tgccgacgag tttctggaag aaaagggcta taaatttagc aagcgattat
     5461 tcaagcacga tccacaaatt gatgctatca tcacaaccga tagcctccta gctgaaggtg
     5521 tttgtaacta tattgccaaa caccagctgg atgtccctgt tctcagcttt gactcggtta
     5581 atcccaagct caacttggca gcctatgtcg atatcaatag tttagagctt ggtcgtgttt
     5641 cccttgaaac tattctccag attattaatg ataataaaaa caataaacaa atttgttacc
     5701 gtcaattgat cgcccacaaa attatcgaaa aataagagac tgggcaaaaa gtcgttaaaa
     5761 gcaaaaacgc atactatcag gtattgaaaa aacttgatac tatgcgtttt attgtgggaa
     5821 gatttacttc cttttctact gaaattgagt cttttcccaa gatcttttta tactcaatga
     5881 aaatcaaagt gcaaactagg aagctagccg caggttgctc aaaacactgt tttgaggttg
     5941 tagatgaaac tgacgaagtc agctcaaaac atggttttga ggttgcagat agaactgacg
     6001 aagtcagctc aaaacatggt tttgaggttg cagatagaac tgacgaagtc agctcaaaac
     6061 atggttttga ggttgcagat agaactgacg aagtcagtaa catatatacg gcaaggtgaa
     6121 gctgacgtgg tttgaagaga ttttcgaaga gtattaatca ctaattatct atctcaacaa
     6181 atcttcctag aatatgaaca ttttccgaga cagagacaaa ggagcttgga tccacttgtg
     6241 tcataatctg tttaaattca ttaaactctg cacgtgtaat gacagtgatt aaaactgcct
     6301 ttctctcgtg attataggtt ccttctgcat cgtggatcat ggttgctccg cggtgcaatt
     6361 ttttatggat tttttcaatt accttctctg gatgatttgt cacaatcatg gcctgcatac
     6421 gcttttgctt agtaaagact gcgtctgtca cacggctaga gacaaagatg gtaatcatag
     6481 aataaagagc gtatttccaa ccaaaggtca aacctgctat cagcatgata gttccattta
     6541 ccaagaaaga aatactaccg acattcttac ccgttttctt acgaatagtc aggctgacga
     6601 tatccgtccc accactggag atattgtttc gaagagcaaa accaatcccc aaacccataa
     6661 caacaccccc aaaaagggaa ttgataatgg gatcctctgt caaggttgcc acagggacaa
     6721 actggataaa gaaggaactc atagataccg tgataaaggt aaagacggtg aacttatggc
     6781 caatctgata ccaagctaag accatcaaag ggaagttaat ggcgtagaag cttagcgaaa
     6841 tcggaatatg aaaaccaaac cagtgattac tcaaggcaga gataatctgt gccagacctg
     6901 ttgcaccact cgaatacaca tgccctggtt ggaaaaagaa attaactgct actgctgata
     6961 aaaaaccata gaccagagag gccgaaatct tctcatcata cttttctcga gagatacttt
     7021 gtaagacacg taaaattttt atctgataag caaagcggcg cagataatag cgccaccgct
     7081 taattcgttt tgtttgtttc atcttcttct acttgtaagc tgagttcctc tagttgtttg
     7141 agagcgactg ttgatggagc ttgtgtcatt gggtcagttg ccttgttgtt cttaggaaag
     7201 gcaatgactt cacggatatt ttcttctcca gcaagcaaca tgacaaaacg gtcaagcccg
     7261 atagccaaac caccgtgtgg tgggaaacca tagtccatgg cttcaagaag gaaaccaaac
     7321 tggtcattgg cttcttcagt tgagaaacca agagccttga acatgcgttc ttgaaggtct
     7381 ttttggttga tacgaaggct accaccacca agctcataac cgttcaagac gatatcgtaa
     7441 gcaatggcac gaaccttagc caaatcacct tctaattcat gagcagtctc ttcctgtgga
     7501 agtgtgaaag gatggtgggc gctcatgtag cggccttctt cttcagacca ttcaaacatc
     7561 ggccagtcaa ccacccaaag gaagttgaac ttatcattat caatcaagcc aagctcttta
     7621 gcaatacgtc cacgaagggc acccagtgtt gcattagcca cttcaagcgt atccgccaca
     7681 aagagaacca agtccttatc ttcaagaaca agcgctgttg tcaattcttc ttggatacca
     7741 gtcaagaact tggcaactgg tccgtttaat tctccatcaa ccaccttgac ccaagcaaga
     7801 cctttggcac catactgttt ggctacttcc gtcatcttgt cgatgtcttt acgtgaatag
     7861 ttgtccgcag ctcctgtgac cacaatcgct tttacagcag gtgcttctga aaagacttta
     7921 aagtctacac ctcggaccac ttctgtcaag tcctgaagca acatgtcaaa acgagtatct
     7981 ggcttgtcag aaccgtaaag agccatagca tcatcgtatt tcatacgagg gaatggtagc
     8041 gttacttcga tgccttttgt ttccttcatc acgcgcgcga tcaagctttc tgtaatatct
     8101 tggatttctt gctcagtaag gaaggacgtt tccaagtcga cctgagtaaa ttcaggctgg
     8161 cggtctccac gcaagtcctc gtcacggaaa catttaacga tttggtagta acggtcaaaa
     8221 ccagcattca tcaagagctg tttcgtgatt tgtggacttt gaggaagagc gtaaaaatgc
     8281 cccttattaa cacgagacgg cactaaataa tcacgcgccc cttcaggcgt tgacttagaa
     8341 aggaatggtg tctccacgtc gataaactcc aactcatcca agtagttgcg gatagagtgg
     8401 gtcaccttgg cacgaagttt aagattttcc aacatttctg gacgacgaag gtcaaggtaa
     8461 cggtaacgca aacgtgtatc gtcatttgcc tcaatgccat ccttaatctc aaatggtgtt
     8521 gtcttagctg tgttaagcac aataagagct gtcacgttta actcaaccgc accagttggc
     8581 aacttatcat tggcttgctc acgcgcagcg acctgaccag tcacctcaat aacaaattcg
     8641 ctacgaaggc tttcagctgt tgccataacc tctgcagata ctttttcagg gttgataacc
     8701 aactgcatga ttccttcacg gtcacgaaga tcgataaaga tcaaaccacc aaggtcacga
     8761 cgacggccaa cccatccttt caaggttatt tcttgtccga tgtgttcctc acgaacacga
     8821 ccagcataca tactacgttt cattatttct ctcctctttt attctgttac tattttacca
     8881 taaaagcgca ggctcttcat gaaaatcatc agaaaagttt gccagtcttt aaaagtcagg
     8941 tgaaagccct aaaaattagc gctaatactc ttcgaaaatc tcttcaaacc acgtcagcgt
     9001 cgccttaccg tatgtatggt tactgacttc gtcagtttca tctacaacct caaaaccatg
     9061 ttttgagctg acttcgtcag ttctatccac aacctcaaaa cagtgttttg agcaacctgc
     9121 ggctagcttc ctagtttgct ctttgatttt cattgagtat aatacaaaaa tccgatgaac
     9181 ttcaccggac tcttttattt tgaatttttg cctgctttac gcttttcagc gatttcggct
     9241 gcctttcgag gcaagacaat ttccgttatg taagccgtcc caaaacgcag tacacctgca
     9301 ataggagcaa agacaactgc tagatagtta tagaagaaat cgcctttgaa ggcataagct
     9361 agcgctccaa tgatgaaaaa tagaacgact gcctgaatca ctgctaataa aattactcgt
     9421 ttcatgtgac ctcctgactc tattatagca tgagaatcat caaaaagccg actaaattat
     9481 tcaaagcgtg aagagaaata ctgtagacca gaccttttct gctaatgtaa gccaaaccca
     9541 aactaaaacc aaggctaaaa tagacaaaaa attgttgcac atcacctgga aaatgaatca
     9601 aggcaaatag aagactagat accagaagaa aaatcagggt tcgtttacta ttgtcctgct
     9661 taggaaagag atagcgtgct aacatccctc taaaaacaat ctcttccgtc aaaggagcaa
     9721 aaataaccac agcaaagaat gagaaaagtg gttgagacaa ggtcaagtct gtcgctattt
     9781 gctgatttac tgaaggatca tctggcaaga agaattgaac gaccagagat aagaaccaaa
     9841 ccaagacagg aagccaaata aatcgattaa agccgctctt ctcaatatga acaggagcct
     9901 tctgatacca tttgtaaatg ccgtacacat atactccagc caaggccaca tagagtagag
     9961 taacagcata gggtgaagcg cctaaagcaa gcgacgcagt cgcgagcccc tgaataaagc
    10021 catagataaa taaaaaggat agaagggcta gaagaatcca gccaaggttt ttaagtaatt
    10081 tcatagataa ctcctttatt tgaaataacg ttttaccata ggtaactgca tcacattgat
    10141 ataaacatgg atggctccta caagcaagaa agaaagtaac tgaatctctc ctgtcaagaa
    10201 agaaatgata ataagaaaaa tatataaggc tggtaagaca tattggtgta attggaataa
    10261 aattcgaaaa ctctgttcca aattagcctg acgctcccct tcatcataag aatttatata
    10321 gttcaagaca tcctttggtg tagcgaaaaa ttccaaatca aactgacgaa caatcgcaat
    10381 ggttttaaaa agagattttt gagcgactaa gaataccaca aagagtaaga aagaaaggaa
    10441 aaatgtttga gggtttgtat gcaatataat cacctcactt aatgaaataa aaatagccaa
    10501 tggaatcgct acacctgtaa tattaaaagc aatggttcca aactcaagat tccgatacat
    10561 ttgcacataa taggtttcat tcagatcgtc atccatttcc tcttgataca aggaatgaaa
    10621 ttttctgctt ttctttaaga aattgaaagt caaaaacata ctaatgaaac ctatcagtaa
    10681 acaaatagct gatatccatg gcatcaaggc ttttacatct aaaataattt cgtgggattc
    10741 gacacgtgcc ttaaacatcc ctacaaacat gcccaagaac cccccaagac aatagacatc
    10801 aaaaataaca atctacgttt ctttttcata ttcattctcc tttttcactt gctagatttt
    10861 tggatttctt ttcaatccat tcaattactg ggatgagagc aaagtagacc caaacaaatt
    10921 ggtcgctttg atagggatta aaccagctta ggtccatccc aatcagtaga aatacgctga
    10981 ctaataaagc tatgaccact acataataaa tcactttata cttgttcatc actcgtcctc
    11041 ctccaaacga aataccgatt cgactgtttc gttgaaaatt tgagatattt tcagggcaat
    11101 gataatggat ggggtgtact catcccgttc tagtaggcta atggtctgtc tggaaacccc
    11161 tgccagtttg gctaggtcgg tttgattgag accatcgcga gctcgaagct cttttagacg
    11221 attttttagt tgcatgttac acacctactc tccgtcaaat tcaacggttt ggatatcctc
    11281 aatacgttgc aacttgaatt tttcttttcc cgtattatct acacgtcgta gctttaccca
    11341 ttcctcatca acatccacaa cttcccagtt atctggccca atatacactc ccgttataat
    11401 tggttccttt ccaatcattt cttgtaataa tctcgacatt tctgcgtttc ctttctcttt
    11461 tcgctcaagt cttttgattt tattctctag tttcttgatt tttttagaat tatta
//
LOCUS       DAVHEX010000065        11402 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVHEX010000065 DAVHEX010000000
VERSION     DAVHEX010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11402
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.065"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            1..1068
                     /locus_tag="VVL45_001814"
                     /pseudo
     CDS             1..1068
                     /locus_tag="VVL45_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1193..1396)
                     /locus_tag="VVL45_001815"
     CDS             complement(1193..1396)
                     /locus_tag="VVL45_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4187949.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(1389..2471)
                     /locus_tag="VVL45_001816"
     CDS             complement(1389..2471)
                     /locus_tag="VVL45_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEU4187950.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFNLFTSTSKDLLYFD"
     gene            complement(2597..3379)
                     /locus_tag="VVL45_001817"
     CDS             complement(2597..3379)
                     /locus_tag="VVL45_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEU4187951.1"
                     /translation="MVYTSLSSKDGNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(3379..4722)
                     /locus_tag="VVL45_001818"
     CDS             complement(3379..4722)
                     /locus_tag="VVL45_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEU4187952.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQNL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(4821..6137)
                     /locus_tag="VVL45_001819"
     CDS             complement(4821..6137)
                     /locus_tag="VVL45_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEU4187953.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYATVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKKELTAETIVINTGAVSNVLPIPGLATSKNIFDSTGIQSLDKLPEKL
                     GILGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            6330..7556
                     /locus_tag="VVL45_001820"
     CDS             6330..7556
                     /locus_tag="VVL45_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEU4187954.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPKVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GLVETFYILGASYFIIMLLASQFIKRPNEQELAILSSSGKEKTASLTQGMAANQALKS
                     NRFYMLWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKALT"
     gene            7755..9329
                     /locus_tag="VVL45_001821"
     CDS             7755..9329
                     /locus_tag="VVL45_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU4187955.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
     gene            9593..10381
                     /gene="codY"
                     /locus_tag="VVL45_001822"
     CDS             9593..10381
                     /gene="codY"
                     /locus_tag="VVL45_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEU4187956.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PIESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            10381..10956
                     /locus_tag="VVL45_001823"
     CDS             10381..10956
                     /locus_tag="VVL45_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEU4187957.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELFE"
     gene            11038..11123
                     /locus_tag="VVL45_001824"
     tRNA            11038..11123
                     /locus_tag="VVL45_001824"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11072..11074,aa:Leu,seq:aag)
     gene            complement(11246..>11402)
                     /locus_tag="VVL45_001825"
     CDS             complement(11246..>11402)
                     /locus_tag="VVL45_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU4187958.1"
                     /translation="YSNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERT
                     KFVLSQA"
BASE COUNT         3377 a         2136 c         2508 g         3381 t
ORIGIN      
        1 aaagtctgac atgaaggctg gactaaagat agagtttctc ttgaatacgg tctcccaagt
       61 cgtacgatac ttcttaactg gctagcacaa tacaggaaaa acgggtatac tattgttgag
      121 aaaacaagag ggagagtacc tgagagcgga gaatgccatc ctaaaaaagt taagagaact
      181 ccgattgaag gaggaaaaag agaaagaaga aagacagaaa ttgttcaaga attaatgact
      241 gagttttcgt tagatcttct tctagaagcc attaaactag ctcgttggac ctactactat
      301 cacttgaaac agctagacaa aacagataaa gaccaagagc ttaaaactga aattcaatcc
      361 atctttatcg aacacaaggg aaattatgct tatcgccggg ttcatttaga actaagaaat
      421 cgtggttatc tggtaaatca taaaagagtt caaggcttga tgaaagtact caatttacaa
      481 gctaaaatgc gaaagaaacg aaaatattct tctcataaag gagacgttgg taagaaggca
      541 gagaatctca ttcaagccca atttgaaggc tctaaaacaa tggaaaagtg ctacacagat
      601 gtgactgaat ttgccattcc agcaagtact caaaagcttt acttatcacc agttttagat
      661 ggctttaaca gcgaaattat tgcttttaat ctttcttgtt cgcctaattt agaacaagta
      721 caaaccatgt tggaacaggc attcacagag aagcactacg agaatacgat tctccatagt
      781 gaccaaggct ggcaatacca acacgattct tatcatcggt tcctagagaa taagggaatt
      841 caagcatcta tgtcacgcaa gggaaacagc caagacaacg gtatgatgga atctttcttt
      901 ggcattttaa aatccgaaat gttttatggc tatgagaaaa catttaaatc acttaaccaa
      961 ttggaacaag tcattataga ctatattgat tactacaaca acaaacgaat taaggtaaaa
     1021 ctaaaaggac ttagtcctgt gcagtacaga actaaatcct tcggataaat taattgtcta
     1081 actgtttggg gtcagtacac aatacctgat actatgcgtt ttattgtggg gagattttcc
     1141 tcattttcta gtgaaattga actttttcta acatcttttg atagatgact tcttaatcaa
     1201 ttatcatttc cattgttgtt tcttggggtt tcatcccttg tctttgataa aagcggagag
     1261 caccctcgtt atcattccag acacttaggg ttagattatt gcagccaatt tctttagcat
     1321 aatagcagaa aaatgacaat aaaatagccc ctagatattt tgaaaggaaa gcataggggc
     1381 tatatagttt aatcaaaata aagcaaatct ttgctggtgc ttgtaaagag gttgaagcca
     1441 tccttggtaa caacaccgca gtcttcaata cgaacaccga ctttaccagg gatatagata
     1501 cctggttcaa cagagaagca catgccttct tcgatgacca tgtcgtttcc ttccatgata
     1561 gatgggaatt catggacatc cataccgata ccatgcccga gacggtggtt gaagtactca
     1621 ccataaccag ctttttcgat gacctcacgg gcagcgcggt ccacttcatg agcagtcaca
     1681 cctggcttga taaagtcaag agcagcttgt tgggcttcaa gagtcaagtt gtaaatatct
     1741 ttcttgaatt ggtctggttt gccgacagcg actgtacgag tcatatctga cgcatagcca
     1801 ttgaccagaa cacccaggtc aaagaggaga agagcatcat tttcaacctt attagctgct
     1861 ggaatgccgt gtggattcgc agcattatca ccagtcaaga ccatggtatc aaagctcatt
     1921 tcataacctt cacgtttcat ggcaaagtcg atttgtgcga tgatatctgt ctcagtctta
     1981 tcaagagaaa tattgtcaaa accaacatga acagccttgt cagcataaag acctgcaacc
     2041 atcatttttt gcacttcatc agctgatttg atgaggcgca tgcgttggat acgaggagtg
     2101 aggttgtcaa actcagcagt ctcaaaaact gttttcaaac catggtattt ggtcaagatg
     2161 agattgtcaa actcaacagc gacacgtttg aagtcaagtt gtggaagagc atgtttgatt
     2221 ttttgccatg gattttcaga atcgacatag cccactactg ggaaggaaac ggtgctactt
     2281 gcacgttcta cttcaagagc tgggacaaag aggagaggtt cctgatctgc taggacaaag
     2341 aggaacattt ggcgttcatg gggatcactg taaaaaccag tgaggtaatt gattgtgacg
     2401 gggtcagata cgacagcgac gtctagtttt tctgattcaa gatatgttag gatttgttgt
     2461 aatttagaca tatgctacct tctttctacc cctctatttt ggcaaaaaat gagagaaaat
     2521 gcaagggaga agggtaaaag aaaagacaaa aaactccgac aagataacgg agttctttga
     2581 tatcattttc ctttgtttaa gaaaagtcag ccttgctttt gacgtcactg tactcttcag
     2641 cgatttcttg gctgagaatg tcctcatagg cagggagttg gatgtcctga ccatatttct
     2701 tcttgagggc agtagtaact aggcgataag ccagattggc attacgagag aggataggcc
     2761 cgtggaagta ggaaccaaag acattcttat aatgaacccc ttcaccgacc ttttcttcgt
     2821 tgtttccatt tccatagaca acctgtccca gcggtttttg gtcatcagag aggaaggtac
     2881 gaccttggtg attttcaaat ccatagtagg tttcatcgaa atcttcattg tgaatcttga
     2941 tgtcaccgat aaaacggtta ttggtctggt tgagcgtgta gtgtcccatg acccctagcc
     3001 cttcgatacg ttttcctgaa gcttcaacat aatattgacc caatagttgg aaaccaccgc
     3061 agatagccag aactacaccg tcgttttgga tgtagttgtc aatgctctct tttttagcag
     3121 gtaggtcgtc tgcaatgata ctttgttcaa agtcttgacc accaccgaaa aaggcgatgt
     3181 cgtagtgatt ttcatcaaag tcatcatgga gagaaacgat gtcaacggtc acatgggctc
     3241 ccagtttttc agccacatac ttgagcatga ggatgtttcc attgtccccg taggtattca
     3301 tgagatttcc gtagaggtgg gcaatgttga gctgataggg gtaattgcca tcttttgagg
     3361 aaagtgaagt ataaaccatt agttcatctc ctttctaaca atctgacgac tagccagcag
     3421 ttcacgaaat tccagcatgg cagtataagt tgccagaata taggcatgct tgcagtcttg
     3481 attctcaatg gtcttgagaa cttgctccag attactcgtt tcagtgattt tctcagctgg
     3541 atagccagtc actcggaggc gacgagcgat ttcagaatga cgaacaccgc cagcgttgat
     3601 ttcaggaatg tccatgtcag tgatttgttc aaagtctgca tcccagatcc agctagtgtc
     3661 aattccatct gcatagttgg cattaaggag gacagatagg ctaaatggat aaggtgctag
     3721 tttgatcatt tcgatagctt gggttgcacc gactggattt ttaatcaaga caagggtaca
     3781 ttccttgtca ccgatatgaa aggtttcttg gcgtccaaag acagcacggc tcttgtcaaa
     3841 tccctgtttg atgagttgcg aatcggcacc taggaaacgg gcgatggcca cagcagctag
     3901 ggcgttatag atattataga gcccgccgat ttggataccg tattcttggc cgtctatgac
     3961 aaagcgagag cgattgttgg tcaactcaac cagttttgtc aaacgatagt cgagatcagg
     4021 acgtttacat ccacaacctt cacagatata ggcacccaag tttgcatagg tattatgctc
     4081 atatttgagg atgccttggc agtcaggaca gagaatccct tcggtattgt agtgagccag
     4141 ttgggctggt cccttttcca agtcaaaacc aaaatactct atagggtttg gaatagttgg
     4201 cttgtagaaa agtggactgt ctccgttaag gagaacagta gcagttggaa ctttccgaat
     4261 ggcatccaat atcatgttat aggtagtata gatttcaccg aaacggtcca tctggtcacg
     4321 gaagatatta gtaatgacaa aaagactagg ctggatatag tcacagatac gagatagact
     4381 ggcttcgtca atttcgagga cggcaatatt tttcccagtt ttagaagatt tggctgttag
     4441 gaaggttgtt gcaatccctg taatcatgtt ggcaccgctt gggttggtta gaacttgacc
     4501 ataaacctct tttaaaatgc cgacagtgag ggcagttgtc agggtttttc catttgttcc
     4561 agtgacaacg acaatctcgt agttcttagc taggttttgt aaaatatctt tatcaaattg
     4621 aagggcgact ttccctggga gcgtacttcc acgtccaaga cggcttaaaa cgaagtggga
     4681 agaacgccca gcaagaaggc ccaaagtagt ttttaagttc atgtttctat tatatcataa
     4741 aagaggagaa agacagattt gagattccgc agaagtaaaa acagcccaaa gagggctgtt
     4801 cgatacgatt aaatctcaac ttaaatcgca aacaagtcat tcaagttctc agccaaggtt
     4861 gggtgagtga agatttgttt tgtgaagtaa gtgtaaggaa tcttgttgtc catagcaaca
     4921 gtgatgatgt tgatgatttc ttgagaacct tctgagaaga tgcttgctcc aagaatttct
     4981 tttgtttcag tattgacaac agctttgaag gcaccgcgaa ggtctccatt tacgtgacca
     5041 cgaggcattg ctgcaacggg gatttcctta acagcgtatg gaagtttcaa atcagctgct
     5101 tggctttcag tcaaaccaac ttgtgaaagt gcaggtgtga tgaacatagt atttggcaca
     5161 ttgagacggt cttcaagtgt atagctgcca tctccagcaa ggtagctgta aacaacacgg
     5221 aagtcatcaa gtgaaatgta agtaaattga aggccaccgt tgacatctcc aactgcaaag
     5281 acaccaggaa cgtttgtttg acagtgtttg tctactttaa tagcaccacg ttcagttagt
     5341 tcaatatctg tattttcaag ttgaagtggt tctacatttg gtttgcgtcc agttgcgtag
     5401 agaagggcgt cgaaacggta agtttcgtct tcagttacga caagcacttg gtcaccatcg
     5461 tttttgattt cagtagtatg gatattttga agcaattcaa tgccatcttc ttccatgtat
     5521 tgtttagcaa gagctgcgat ggaaggttct gcacgaggta ggaatgtatc caaggcatct
     5581 aggactgtga ccttgcttcc aagtttgttg taaaggccgg caaattcaag accgatattt
     5641 ccgccaccaa ggattccaag tttttcaggt aatttgtcca agctttggat acctgttgag
     5701 tcaaagatgt ttttgcttgt agcaagtcca gggattggca agacgtttga aacagcacca
     5761 gtgttgatga cgattgtttc agcagtcagt tctttctttt catcaccagc ttggatttcg
     5821 atgactttat ttgaaaggaa gtgagcttcc gcatcaaaga tatctacgcc tgtaccagca
     5881 acagtcgcat agtttttacc gttgaggcga ccagtgatcg tgtttttagt agcaatgact
     5941 tcttcaaaag acaagtcctt ttcagcagca actagcaagg ttttagttgg gatacaacca
     6001 atgttgatac aagttccacc gtacatagcc ttgctgcgtt caacgagggc aacttttttg
     6061 ccagctgaag ccaatttacc tgctagtgtt ttaccagctt taccaaatcc aataacaatt
     6121 aaatcgtatg ttaacattta tcttcctcct attcgaattt cattctagca caagaaaaaa
     6181 acttttgcac aattctgcta cggaaaagac agaaaaagat aaaaacgctt tccagacaaa
     6241 aaactagtaa ttttttgaca agcttgataa actaaaatcc tatctatttt tatataagat
     6301 agaataaatg ttcgaaaata ggaggtttta tgaaatcgaa tcgttatatt attgcctttg
     6361 ctggggtcat tttacactta atgctgggtt cgacttatgc ttggagtgtt tatcgtaacc
     6421 ctattattga aaaaacggga tgggatcagg cttctgttgc cttcgccttt agtctagcaa
     6481 tcttttgttt gggcttatcg gctgcattta tggggcgttt ggtagaaaaa tttggtccga
     6541 aagtcatggg aagtctatct gcttttctat acgcaggtgg aaatatctta acaggatttg
     6601 caatagaccg tcaggagctg tggttgttgt atctcgctta tggcatttta ggtgggcttg
     6661 gtttgggagc aggctatatt acccctgtgt cgacgattat aaaatggttt cctgataaac
     6721 gtggtctcgc aacaggttta gcgattatgg ggtttggttt tgcttcttta ttgactagtc
     6781 ccatagcgca acacctcatc gcaggggtag ggcttgtaga aactttttat attttaggag
     6841 caagttactt tattatcatg ctcctagctt cacaattcat taagcgtcca aatgagcaag
     6901 agcttgcaat tttatcttct tcagggaaag aaaaaacagc ctctttgacg caaggaatgg
     6961 ctgcaaatca ggctctaaaa agcaatcggt tttatatgct ttggattatt ttctttatca
     7021 acatagcttg tggtttaggc ttaatttcag cggcatcgcc aatggcacag gagatggctg
     7081 gcttgtctac aagtcatgca gcagtaatgg tgggtgtttt ggggattttc aatggatttg
     7141 gtcgcttgct ctgggcgagt ttgtctgact atatcggtcg ccctctaacc tttagtatat
     7201 tactgcttgt caatcttttc ttttctctct cactttggct ctttacagat tccgttttat
     7261 ttgtagttgc tatgtctatt ttgatgactt gctatggagc tggtttttct ttgattccag
     7321 cttatctcag tgatattttt ggaaccaagg aattggccgc tctgcatggt tatattttaa
     7381 cagcttgggc aatggctggt ttagcgggac ctattttatt agcagagact tataaaatgg
     7441 ctcattcgta cacacaaacc ttgttcgttt ttctcatttt atacagtatc gccttggctt
     7501 tgtcttatta tctaggtcgt tcaatcaaaa aagaaagtca aaaagcgctt acatgatttt
     7561 ctgtagaaat ggggctatct tttgctctgg tcttgtgtta tactagatag gttgcaaaga
     7621 aaacagtact tttcttttgt ggaaaagaag caacacgatt ttatatctag ttatatgaaa
     7681 atacgtcata aaaagaaaag tataaactaa gccctatagg gtggcttcgc accaccttta
     7741 gaaagaagaa taacgtgaaa tttaatgaat taaacttgtc tgctgatttg ctagcagaaa
     7801 ttgaaaaagc tggttttgta gaagctagtc ctatccaaga acaaactatt cctttggccc
     7861 ttgaaggcaa ggatgttatc ggtcaagctc agactggtac aggaaaaact gcagcctttg
     7921 gcttgcctac ccttgaaaaa atccgtacag aagaagcgac tatccaagcc ttggtcatcg
     7981 ctccaactcg tgaactagct gtccaaagtc aagaagaact cttccgcttt ggtcgtagta
     8041 agggagtcaa agtccgttca gtatatggcg gatcaagcat tgaaaaacaa attaaggctc
     8101 ttaaatctgg tgcccatatt gtggtgggaa ctccaggtcg cctcttggac ttgattaaac
     8161 gcaaggcctt gaaattacaa gacattgaaa cccttatcct tgacgaagcg gatgaaatgc
     8221 ttaacatggg cttccttgaa gacatcgaag ccattatttc ccgtgtacct gagaaccgtc
     8281 aaactttgct tttctcagca actatgccag atgccatcaa acgtatcggt gttcagttta
     8341 tgaaagcccc tgaacatgtc aagattgcgg ctaaggaatt gacaacagaa ttggttgacc
     8401 agtactatat ccgtgttaag gaacaagaaa aatttgacac catgactcgt ctcatggatg
     8461 tggcacaacc agaactcgct attgtatttg gtcgtaccaa acgccgtgtg gatgaattga
     8521 ctcgtggttt gaaaattcgt ggcttccgtg cagaaggaat tcatggcgac ctagaccaaa
     8581 acaaacgtct tcgtgtcctt cgtgacttta aaaatggcaa tcttgatgtt ttggttgcga
     8641 cagacgttgc agcgcgtggt ttggatattt caggtgtgac ccatgtctac aactacgata
     8701 ttccacaaga tcctgagagt tatgttcacc gtatcggtcg tacaggtcgt gctggtaagt
     8761 caggtcaatc tattactttt gttgctccaa acgaaatggg ttaccttcaa atcattgaaa
     8821 acttgactaa gaaacgcatg aaaggtctca aacctgcaag tgtagaagaa tccttccaat
     8881 caaaaaaaca ggtagctctc aagaaaatcg aacgtgattt tgcagatgaa accattcgtg
     8941 ccaactttga gaaatttggt aaggatgctc gcaaattggc tgctgagttt actccagaag
     9001 aattggcaat gtatatcttg agtctgacag tccaagaccc agatagcctt ccagaagtgg
     9061 agattgcacg tgaaaaacca ctaccgttta aaccatcagg taatggtttc ggtggtaaag
     9121 ctaagggagg tcgtggaggc cgtcgtgggg acgaccgtcg agagcgtgat cgccgtggca
     9181 atggtcgccg tgatgagttc aaaaaaggaa gtcgtggcaa cgatcgtttt gataaggaaa
     9241 aacgttaccg taaggataat aaaaaaccac gcaatacttt aagcgaaaag caaacaggct
     9301 ttgttattcg taacaaaggc gataaataag aaaaagcggt tcaaaatgaa tcgctttttt
     9361 atataaggag accgaatgtg aaaagacaaa taaaattatt atattatctt ttataatatt
     9421 atgatacaca aaaataggca acttgtcaat agaaaaggaa caatttaatt aaaaatattc
     9481 tggtttttaa ttaaattgta agtcaatgca agtcaataag gaattttcag ataattattg
     9541 aaaaagagac gtttttatgt tataatgata gcgattaaat tcgaggtgaa aaatggcaca
     9601 tttattagaa aaaactagaa aaattacatc aattttgaaa cgctcagagg agcagttgca
     9661 ggatgagctc ccttacaacg ctattacgcg tcagttagcg gatattattc attgcaatgc
     9721 ctgcattatc aatagtaagg gacgtctgct tggctatttt atgcgttata aaacaaatac
     9781 agatcgcgta gagcaattct tccaaactaa gattttccca gatgactacg ttcaaggggc
     9841 taatatgatt tacgaaacag aagcaaactt gcctgttgag catgatatga gtattttccc
     9901 tattgagagt agagatgatt ttccagatgg cttgacgact attgcaccga ttcatgtatc
     9961 ggggattcgc cttggttctt tgattatttg gcgtaatgat aaaaaattcg aagatgagga
    10021 cttggttctt gttgagattg ccagtaccgt tgttgggatt cagcttctta acttccaacg
    10081 tgaagaagat gagaaaaata ttcgtcgtcg tactgctgtc accatggcgg tcaataccct
    10141 ttcttactcc gaactccgtg ctgtttcagc aattttaggg gaattaaatg gaaatgaagg
    10201 gaagttgact gcgtcagtga ttgcagatcg tatcggaatc actcgctctg tgattgtcaa
    10261 tgctcttcgt aaacttgagt ctgcggggat tattgaaagt cgctcacttg gaatgaaggg
    10321 aacctatctt aaggtcttga tttcagatat ttttgaagaa gtgaagaaaa gagattacta
    10381 atgacaaagg ctttaatttc gattgattat acagaagatt ttgttgctga tagtgggaaa
    10441 ctaacagcag gtgctccagc tcaggcgatt tcggatgcca tcagcaaggt aactcgatta
    10501 gcttttgaac gaggagatta tatcttcttt actattgatg ctcatgaaga aaacgattgt
    10561 ttccatccgg aaagtaagtt atttcctcct cataatctga ttgggacgag tggacggaat
    10621 ttatatggag atttggggat cttttatcaa gagcatggtt cagacagtcg tgtcttttgg
    10681 atggataaac gccattactc agctttttca gggactgacc tagatattcg tttgagagag
    10741 cgtagagtgt ctaccgttat cttaacaggt gtcttgacgg atatctgtgt cctacataca
    10801 gctatagatg cctataatct aggatatgac atcgagattg ttaaaccagc tgttgcttcc
    10861 atctggcctg aaaatcatca atttgcccta ggtcatttca aaaatacact cggagctaag
    10921 ttagtagatg aaaatctaaa tgaacttttt gagtaacctg gttggtgaaa atgaaaaaat
    10981 ttgaaaaaaa gtgttgacaa gcatcccaaa agttgatata ctagtaaagt aatcgacgcg
    11041 gggatggcgg aattggcaga cgcgcaggac taaggatcct gtgaccgctt taggtcgtga
    11101 gggttcaagt ccctctctcc gcataggaaa agagctagct aaagctagct cttttgtatt
    11161 tctctaaaaa attagaatcc agtcaaatgt agtggagaat cagcgaaaat aggctctttg
    11221 tcaactgtag tgggttgaag aaaagctaag cttgagaaag gacaaatttc gtcctttctt
    11281 ttttgatgtt cagagcgata aaaatccgtt ttttgaagtt ttcaaagttt cgaaaaccaa
    11341 aggcattgcg tttgataagt ttgatgagat tattggtcgc ttccaatttg gcgtttgaat
    11401 ag
//
LOCUS       DAVHEX010000066        11016 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVHEX010000066 DAVHEX010000000
VERSION     DAVHEX010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11016
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.066"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(<1..1110)
                     /gene="phtB"
                     /locus_tag="VVL45_001826"
     CDS             complement(<1..1110)
                     /gene="phtB"
                     /locus_tag="VVL45_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtB"
                     /protein_id="HEU4187959.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRYQAGQDKKESNRVAYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVNGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKNEL
                     SASELAAAEAYWNGKQGSRPSSSSSYNANPAQPRLSENHNLTVTPTYHQNQGENISSL
                     LRELYAKPLSERHVESDGLIFDPAQITSRTARGVAVPHGNHYHFIPYEQMSELEKRIA
                     RIIPLRYRSNHWVPDSRPEEPSPQPTPEPSPSPQPA"
     gene            complement(1268..3718)
                     /gene="phtA"
                     /locus_tag="VVL45_001827"
     CDS             complement(1268..3718)
                     /gene="phtA"
                     /locus_tag="VVL45_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HEU4187960.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWVPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHVESDGLVFDPAQITSRTARGVAVPHGDHYHF
                     IPYSQMSELEERIARIIPLRYRSNHWVPDSRPEQPSPQPTPEPSPGPQPAPNLKIDSN
                     SSLVSQLVRKVGEGYVFEEKGISRYVFAKDLPSETVKNLESKLSKQESVSHTLTAKKE
                     NVAPRDQEFYDKAYNLLTEAHKALFENKGRNSDFQALDKLLERLNDESTNKEKLVDDL
                     LAFLAPITHPERLGKPNSQIEYTEDEVRIAQLADKYTTSDGYIFDEHDIISDEGDAYV
                     TPHMGHSHWIGKDSLSDKEKVAAQAYTKEKGILPPSPDADVKANPTGDSAAAIYNRVK
                     GEKRIPLVRLPYMVEHTVEVKNGNLIIPHKDHYHNIKFAWFDDHTYKAPNGYTLEDLF
                     ATIKYYVEHPDERPHSNDGWGNASEHVLGKKDHSEDPNKNFKADEEPVEETPAEPEVP
                     QVETEKVEAQLKEAEVLLAKVTDSSLKANATETLAGLRNNLTLQIMDNNSIMAEAEKL
                     LALLKGSNPSSVSKEKIN"
     gene            complement(3972..5705)
                     /gene="ptsP"
                     /locus_tag="VVL45_001828"
     CDS             complement(3972..5705)
                     /gene="ptsP"
                     /locus_tag="VVL45_001828"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEU4187961.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            complement(5711..5974)
                     /locus_tag="VVL45_001829"
     CDS             complement(5711..5974)
                     /locus_tag="VVL45_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEU4187962.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            6325..6543
                     /gene="nrdH"
                     /locus_tag="VVL45_001830"
     CDS             6325..6543
                     /gene="nrdH"
                     /locus_tag="VVL45_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEU4187963.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
     gene            6625..8784
                     /gene="nrdE"
                     /locus_tag="VVL45_001831"
     CDS             6625..8784
                     /gene="nrdE"
                     /locus_tag="VVL45_001831"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEU4187964.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            8972..9934
                     /gene="nrdF"
                     /locus_tag="VVL45_001832"
     CDS             8972..9934
                     /gene="nrdF"
                     /locus_tag="VVL45_001832"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEU4187965.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            10222..10983
                     /locus_tag="VVL45_001833"
     CDS             10222..10983
                     /locus_tag="VVL45_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU4187966.1"
                     /translation="MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDF
                     YTFYNVSNLDTIVSDSKLSDSILFKLSKHIKVIKP"
BASE COUNT         3180 a         2274 c         2058 g         3504 t
ORIGIN      
        1 agctggttgc ggacttggac taggttctgg agtcggttgt ggacttggtt cttctggtct
       61 tgaatctggt acccaatggt ttgaacgata acgaagggga ataatacgag caattcgttt
      121 ttccaattca gacatttgtt cataagggat aaagtggtaa tggttaccat gagggacagc
      181 tacacctctg gcggttcgac ttgtgatttg cgctgggtcg aaaataaggc catcagattc
      241 cacatggcgt tctgataagg gtttagcata caattcacgt aaaaggcttg aaatgttttc
      301 cccttgattt tgatgataag ttggagtgac agtcagattg tggttctctg acaatcttgg
      361 ttgagctgga tttgcattat aactagaact tgaagaagga cgagatccct gcttcccatt
      421 ccaataggct tctgcagcag ctaactcgct agctgataac tcattcttag gaatgtaatg
      481 gtaatggtcg ccgtgaggaa cgatataagc atcacccgtg tcctcaatga tatcagatgc
      541 attgaagata tacccatcat ccgttgtata acgtccttgg gctctggctg cagcaacagc
      601 attatctgct cttgagttat gattatgact gcgttcctgc ttctgacgtt taatctcttc
      661 ttttgtccga atattatccg catgagctgc atccttaagg taaacatagt atttaccgtt
      721 taccttaatg acataaccac ccttgatttc attgacaatg tctgaatcct tcaactgata
      781 attcggatct ttcatgagga gctcttcact gatgatggca tcataaggaa ccttgccatt
      841 atagtaatga taatggtctc catgagaggt cacataacct tgatccgtaa tcttgataac
      901 aatttgttcg gcgttgatcc cctccctctt actgacttca tctggtgtca agttttctgc
      961 cttttgacca gcctgatcac catctatata agcaactcga ttagactctt tcttatcctg
     1021 accagcttgg taacgtccaa gctcatagga acaaacactt agggcaagga ctgccactga
     1081 acctgctaga tattttttat taattttcat tctttcctct tttcttaagt atatttgatt
     1141 cactataaag taataaaccg gttaattaac tagttaatag tttactcttt ttaatattgc
     1201 ttgtcaagaa ttttacatat atacataata aaaatgaaag cctctttatc gagactttca
     1261 tttttcatta gtttattttt tccttactta cagatgaagg attacttcct tttaacaacg
     1321 caagtaattt ttctgcttct gccatgatac tattgttatc cataatttga agagtcaaat
     1381 tatttcgtaa accagctaga gtttctgttg cattggcttt cagactagaa tccgttactt
     1441 tcgcaagcaa aacttctgct tctttgagtt gggcttctac tttttcagtc tctacttgag
     1501 ggacttctgg ctcagcaggt gtttcctcta ctggctcttc atccgctttg aagttcttat
     1561 ttggatcttc actgtggtct ttcttgccta acacatgctc actggcattg ccccatccat
     1621 cattagaatg tggacgttcg tcagggtgtt ctacgtagta cttaatcgtc gcaaacaaat
     1681 cttccaaggt atagccattt ggagctttgt atgtgtgatc atcaaaccaa gcaaatttaa
     1741 tattatggta atgatcctta tgaggaataa tcaaattacc gtttttaacc tcaactgtat
     1801 gctcaaccat atatggaagt cgaacgagtg gaattcgttt ttcccctttc acacgattgt
     1861 aaatagctgc tgcactatct ccagttggat ttgctttaac atctgcgtct ggagatggag
     1921 gtaggatacc tttttcttta gtataggctt gagctgcaac tttttcctta tcagaaaggc
     1981 tatcttttcc aatccagtga ctatggccca tatgaggcgt tacatatgca tctccttcat
     2041 cactgattat atcatgttca tcaaaaatgt aaccatctga cgttgtatac ttatcagcta
     2101 attgagcaat acgaacttcg tcttcagtat actcaatttg agaatttggt ttgccaagtc
     2161 gctctggatg ggtaattggt gctaggaatg ccaataaatc atctaccaat ttttctttat
     2221 tagtcgattc atcattcaag cgttctaata atttgtctaa ggcttggaaa tcagaattac
     2281 gacccttatt ttcaaacaag gctttatgag cctcagttaa cagattatat gctttatcat
     2341 aaaattcttg gtcacgagga gcaacatttt cttttttagc agttaaagtg tgtgaaacac
     2401 tctcttgttt tgataacttg ctttcaagat ttttaacagt ttcagatggt aaatctttcg
     2461 caaagacata acgagagatg cccttttctt cgaatacata tccttcccca acttttcgta
     2521 ccagctgact aaccaaagaa gaatttgagt ctattttaag atttggtgca ggttgcgggc
     2581 ctggactagg ttccggagtc ggttgtggac ttggttgttc tggccttgaa tctggtaccc
     2641 aatggtttga acgataacga aggggaataa tacgagcgat tcgttcttcc aattcagaca
     2701 tttgagagta agggatgaag tggtaatgat ctccgtgtgg cactgcaaca cctctagctg
     2761 ttcgacttgt gatttgtgct ggatcaaaga caaggccatc agattctaca tgtcgttgac
     2821 tcaaaggcag tttgtagagc tgtttcaaga gactatcaat gtcattactt tgacttgctt
     2881 gactgttagt gttgctgttg ttgcttgtgt tagtatttgt agttcctgga ttgcttacag
     2941 aaggtaccca gtttgttctt gaagtgttat cgctattttg tcggcgatag gttcttgaat
     3001 ttgacagatt tcctcgacca gataggaagg cttctgcagc agccaactcg ctagctgata
     3061 actcattctt aggaatgtaa tggtaatgat ctccatgagg aacgatataa gcatcaccag
     3121 tatcctctat gatatcagaa gcattaaaga tataaccatc atctgtagta tagcgtcctt
     3181 gcgaacgtgc caaggcaaca gcaccatcgt ttcttggagt tccaccttca cgatgttgac
     3241 tatgctcttg tttttgtcga ttgatttcct cttttgtacg gacgttatcc gcgtgggcag
     3301 catccttaag gtaaacatag tattttccat ctaccttgat aacatatcca cccttgacct
     3361 cattaacaat atcctcatct tttagcttat agtttggatc tttcatgagt aattcttcac
     3421 tgatgatagc gtcataagga accttaccat tgtaataatg atagtggtcg ccatgtgaag
     3481 tgacatagcc ttggtctgtt atcttgatga cgatttgctc agcattgatt ccttcacgct
     3541 tgctaacctc atcaggagtc aaattctccg ttttttgcgt cgcttgtttt ccatctatat
     3601 aggaaacacg attattttcc ttaaccgttc tagcttgata cagtcccaac tcgtaagaac
     3661 aaacacttaa aatcaaagct gccgcagaac caacaaggta tttcttatta attttcatcg
     3721 aaactctctt tctatttgat atttctagtt gcaattcatt tctaaattgc agaaactcct
     3781 ctctattact taactggtta atagtttact cctaaatctc caacttgtca agaattttat
     3841 gaaccattta agtatttttt gttctgacca ctactttttc aaggttggac tttctataag
     3901 aaagattttc ttgaaaattc tttttaaaat atcaaaaaaa tccctgtaac tgagttgcag
     3961 ggacttttcg attaatcaaa attaacgtat tctttttgaa gttcaagaac ttcttccatt
     4021 gttgagcatt ctgtaagggc acggtttgcg tactcttcca tcttagctgt gtcgagtttc
     4081 ttcatcaagc tgcgtgtacg aagtacagat gttgctgaca tagagaactc atccaagccc
     4141 attccgacaa gaagtggaac agcttgttgg tcaccagcca tctcaccaca cataccagcc
     4201 catttacctt cagcgtgagc tgctttgatc acattgttaa tcaagcgtag gattgatggg
     4261 ttgtatggtt ggtaaaggta tgaaacttgt tcgttcatac ggtctgctgc cattgtatat
     4321 tggatcaagt cgtttgtacc aattgagaag aagtcaactt ctttagcaaa ttggtctgca
     4381 agcatagccg ctgcaggaat ctcgatcatg ataccaactt ggatgttatc cgcaactgca
     4441 acaccttcag caagaaggtt tgctttttct tcatcaaaga ctgctttcgc tgcacggaat
     4501 tctttcaaga gcgcaaccat tgggaacatg atacgcaatt gaccgtgaac agacgcacga
     4561 agaagagcac ggatttgtgt gcggaacata gcatctccag tctcagagat agagatacga
     4621 agagcacgga atccaaggaa tgggttcatt tcgtgaggca tatcgaagta aggaagttcc
     4681 ttatctccac cgatatccat tgtacgaacg acaacaggtt taccgttcat tccttcaaga
     4741 acagccttgt atgcttcata ctgctcatct tcagttggga agtcttgaga atccatgtac
     4801 aagaactctg tacggtaaag tccaacagct tctgcaccgt tgttgttaac accttcaacg
     4861 tcttttggag taccgatatt agcagccaac tcgaagtgtt taccgtcagc agtcactgtt
     4921 tgagcatctt tcaaaagtgc ccattcagct ttttgtttcg cataggcttc accagctgct
     4981 ttaaattctg ccgcttgttc atctgttggg ttgataatca cttctccagt gatcccgtta
     5041 acagcaagga tgtcaccgtc tttaacgatt tcagtgatgt tatttgtacc taatacagca
     5101 gcaatttcaa gtgtacgtgc catgatagct gagtggcttg tacgtccacc aatgttggtt
     5161 acaaaagctt ttacaaagtt tttgtccaat tgagctgtat ctgaaggagt caagtcatgc
     5221 gcaatcacaa tcacttcttc attgatagaa gctgggtttg gcaatttttt accaagaagg
     5281 tttgccaata cacgttttgt cacgtcgcgg atatccgctg cgcgttcttg catgtatggg
     5341 ttgtcttcca tgccttcaaa gatagtgata aacatatctg taacttcttt cagacctgct
     5401 tctgcattca ctttcttcgc acggatagtt tccttgattt ggctgatcat ttctgggtca
     5461 gcaagaacca ttaagtgagc atcaaaaact tgagctgctt cttcaccgag cgtacctact
     5521 gctttctcgc gaataacaga aagctcgtct tgtgatgcct gtagagcggc atcaaggcga
     5581 gcttcttctg cgtttgtatc ttcgactgta atagtctcaa atgacaaatc cggctgaacg
     5641 agtagatatg cttttgcaac tgcaacaccg tcagatgctg cgattccttt aagcatttct
     5701 gtcatttccc ttatgccaat ccttcttttt ccattgtttc tgagattgca gcgatagcgt
     5761 catctgcatc tgcaccttca gctgagatag ttacgtcagc accttggcca acaccaagac
     5821 tcataacacc cataattgat ttaaggttaa ctgatttacc tttgtactca agagtgatat
     5881 ctgaagcaaa tttgctagca gtttgtacca acaatgttgc tggacgtgcg tgaatacctg
     5941 tttctgccac tacgtggaaa tctttagaag ccatagtttg actctccttt tgttctttct
     6001 ttttttgagt tatatgtgat aacccttaca atttagtatt ataccatctt ctaggatttt
     6061 tttcaagcat tttctggatt ttccttcaat ttcctttcag ataacttgct gaaaatcttc
     6121 tatcctagat aacaaaacac aggatatagt ttctttaaaa acaatttata ggataatcat
     6181 tgctatttca caatacaaac actatatatt gtgtttatat ataaaaacaa caaaagaaca
     6241 ccactatatt tagttcaaat cattgacaaa attttatttt ctgatatact aagatagtat
     6301 taaattttga agaggagtta cacaatggta accgtttatt ctaaaaacaa ttgtgtccaa
     6361 tgtaaaatga ccaagcgttt cttggacagt aataatgtct cttatcgtga aatcaatctt
     6421 gatgagcaac ctgagtacgt cgatcaagtt aaagagctcg gttttagcgc agctcctgtt
     6481 atccaaacac caactgaagt cttttcaggt ttccaaccag gaaaactgaa acaattagca
     6541 taatcttagt acatcatcca gaagaaactg cttttagggc taacttagaa gcctttcttt
     6601 tgtaattaga ataagggaaa ttttatggga ttaaaacatc ttgaagacgt gacttacttc
     6661 cgtcttaata acgaaattaa ccgtcctgtt aatggacaaa tcatgcttca taaagataag
     6721 gaagccttgg atgctttctt taaagaaaat gtagttccaa acactatggt ttttgattca
     6781 atcaaagaca aaatcaatta cctcattgaa cacaactaca tcgaaacagc ctttatcaag
     6841 aaataccgtc cagagttctt ggaagaattg gctcaattta tcaaagacca aaacttccaa
     6901 ttcaagtcat tcatggcagc ttataaattt tacaatcaat atgccttaaa gactaatgac
     6961 ggtgaatact atcttgaaaa tatggaagac cgtgtcttct tcaacgccct ttatttcgct
     7021 gatgggaatg aagctgttgc aatcgatatt gccaatgaaa tcatccacca acgctaccaa
     7081 cctgctactc cttccttctt gaatgctgga cgtgctcgtc gtggggagtt ggtatcttgt
     7141 ttcctaatcc aagtgacgga tgatatgaac tctatcggac gttctatcaa ctcagctctt
     7201 caactttcac gtattggtgg tggtgtggga attaccctca gcaaccttcg tgaagctggt
     7261 gcacctatca aaggctatga aggagctgct tcaggtgtcg ttcctgttat gaagcttttt
     7321 gaagacagct tctcttactc caaccaattg gggcaacgtc aaggtgccgg tgttgtctac
     7381 ctcaacgtct ttcacccaga tatcatcgct ttcctttcaa ctaagaaaga aaacgctgat
     7441 gaaaaagtac gtgtcaagac tctatcactt ggtgttgtag tacctgataa attctacgaa
     7501 ttggctcgta aaaatgaaga aatgtacctc ttcagccctt actctgtaga aaaagaatac
     7561 ggtgtgccat tcaattacat cgacattact gaaaaatacg atgaattggt cgcaaatcca
     7621 aatattcgca agacaaaaat caaggcgcgg gatttggaaa ctgaaatctc taaattgcaa
     7681 caagagtctg gctatcctta tgtagtcaac atagatacgg ctaaccgtgc aaatcctgtt
     7741 gatggaaaga ttatcatgag taacttgtgt tctgagattc ttcaagttca agaaccaagc
     7801 ttgatcaacg atgctcaaga attccttcaa atgggaacag atgtttcatg taacctgggt
     7861 tcaaccaacg tggttaacat gatgacttca cctgattttg gtcgttctat ccgtgctatg
     7921 gttcgtgccc ttactttcgt tacagatagt tcacatatcg tagctgtacc aacgattgac
     7981 catggaaata gccaagccca cacctttggt ctcggtgcca tgggactgca cagctacctt
     8041 gcccaacaac tcattgaata tggttcacct gagtctgttg aatttacaag catctacttt
     8101 atgcttatga actactggac cttggtagag tcaaacaata ttgcgcgtga acgtggtatt
     8161 accttccaca actttgaaaa atcagactat gctaacggaa gttactttga caagtatgta
     8221 actggtgaat ttgttccaac atcagaccgt gttaaagaac tcttcaaaaa tgtctttatc
     8281 cctggtgttg ctgattgggc tgaacttcgc gacaaggttc aagaagatgg tctttaccac
     8341 caaaatcgcc ttgctgtagc gccaaatggt tctatcagct atatcaacga cgtttctgct
     8401 tctatccacc cgattacgca acgtatcgaa gaacgccaag agaagaaaat tggtaaaatc
     8461 tactaccctg ctgctggctt gtctacagaa accattcctt actacacttc tgcctacgat
     8521 atggatatgc gtaaagtcat cgatgtttac gctgctgcga ctgaacacgt ggatcaagga
     8581 ctttcactca ccctcttcat gcgtagtgac attccaaaag gcctttacga atggaagagg
     8641 gaaaataaac aaacgacacg tgacttgtcc atccttcgta actatgcctt taacaaggga
     8701 atcaagtcta tctactacgt ccgtaccttt acagatgacg gtggagaagt cggcgccaac
     8761 caatgtgaaa gctgtgtgat ttagtttcta gcatagaaac actatttttc cctaagcagc
     8821 actgataaaa tcaaatctac tatgtcataa cataatttat gtaacatttt aaaagtcggg
     8881 cttccgactt tttgtgcgat actcctatag aattatggta aagtagaaat aattgtgagt
     8941 tcgcaatact aaacacagaa agagatttta aatggaaact tactacaaag ccattaactg
     9001 gaatgccatc gaagatgtca tcgacaaatc aacttgggaa aaactgacgg agcaattttg
     9061 gctcgataca cgtattccct tatcaaacga cttggatgac tggagaaagc tatctaacaa
     9121 agagaaagac ttggtcggaa aagtttttgg tggtttaacc cttcttgaca ctatgcaatc
     9181 tgaaactggg gttcaagccc ttcgcgcaga catccgtaca ccacatgagg aagctgtctt
     9241 caataacatc caatttatgg aatctgtcca cgctaaatct tactcatcaa tcttttctac
     9301 cttgaatact aaggctgaga ttgaagaaat tttcgaatgg accaatacca atccttacct
     9361 acaaaagaag gctgagattg tcaacgaaat ctacctaaac ggcagcccac ttgaaaagaa
     9421 agttgccagc gtcttcctcg aaaccttcct cttctactct ggtttcttca ctcccctcta
     9481 ctatctcggt aacaacaaac ttgccaacgt tgcagaaatc attaaattga ttattcgtga
     9541 cgagtctgtt cacggaacct acattggtta caaattccaa cttggtttca atgaattacc
     9601 tgaagaagag caagaaaaac tcaaagaatg gatgtacgac ctactctata ctctttatga
     9661 aaatgaagaa ggctacacag agagtcttta tgacggtgtt ggttggactg aagaggttaa
     9721 aaccttcctt cgctacaatg ccaataaagc tctcatgaac atgggacaag atccactttt
     9781 cccagattca gctgaagatg tcaacccaat cgttatgaac ggtatttcaa caggaacttc
     9841 caaccatgac ttcttctctc aagtcggaaa tggttacctt cttggcgaag ttgaagccat
     9901 gcaagatgat gactacaact acggtttgga ctaatactct tcgaaaatca aattcaaacc
     9961 acgtcagctt cgccttgccg tactcaagta cagcctgcgg ctagcttcct agtttgctct
    10021 ttgattttca ttgagtataa ttctccacca ctcttaacca tcaaaaagta aactagaaac
    10081 aacaaaataa taacttaata aaaatatcga aagttttgaa aataaacctc gatatttttg
    10141 tttttgtttt attttgttgt ttttgtattt gaatgatgag ctagtttatg gtaagatagt
    10201 agtaggaaac gaggtgataa tatgctcaaa caagaaaaat taaccaaaat tttagagata
    10261 gtaaatagta aaggaaccat aacagttaaa cagattatgg acgaaatagc cgtttcagat
    10321 atgactgcaa ggcgctattt acaggaatta gctgataaag atttgctgat tcgtgtgcat
    10381 ggtggagctg aaaaacttcg aaccaactcc cttttgacta atgagcgatc aaatattgaa
    10441 aaacaagccc tccaaacggc agaaaaacaa gaaatagccc attttgcagg cagtctagta
    10501 gaagaaagag aaactatttt cattggacca ggaacaacat tagagttttt tgcgcgtgag
    10561 ttgcctattg acaatatccg cgtcgtaacc aacagtctac ctgtttttct gattttaagc
    10621 gaacgaaaat taacagattt gattttaata ggtggaaatt atcgcgatat tacaggtgct
    10681 tttgttggta cattgaccct acaaaatctc tctaatctcc aattttctaa agctttcgtt
    10741 agctgtaatg gtattcaaaa cggagctcta gctactttta gcgaggaaga gggagaggct
    10801 caacgcatcg ctttaaataa ttctaataaa aaatatttac tcgcagatca tagcaagttc
    10861 aataagtttg atttttatac tttttataat gtatcaaatc ttgatactat tgtttcagat
    10921 tctaaactaa gtgattcaat cctttttaag ctatctaaac acattaaagt catcaagcct
    10981 taataaactg aaagagcctg ggtaacaact caattt
//
LOCUS       DAVHEX010000067        10744 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVHEX010000067 DAVHEX010000000
VERSION     DAVHEX010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10744
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.067"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(105..1529)
                     /locus_tag="VVL45_001834"
     CDS             complement(105..1529)
                     /locus_tag="VVL45_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU4187967.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAVERAAYLHKTAAILERDKEEIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEATSKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2074..4353)
                     /gene="pulA"
                     /locus_tag="VVL45_001835"
     CDS             complement(2074..4353)
                     /gene="pulA"
                     /locus_tag="VVL45_001835"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEU4187968.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKTYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGYLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4465..6423)
                     /gene="ligA"
                     /locus_tag="VVL45_001836"
     CDS             complement(4465..6423)
                     /gene="ligA"
                     /locus_tag="VVL45_001836"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEU4187969.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KASQLLLQYFHSIENLYQADSEEVASIESLGGVIAKSLQTYFAAEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSVSKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6516..7694)
                     /locus_tag="VVL45_001837"
     CDS             complement(6516..7694)
                     /locus_tag="VVL45_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU4187970.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(7774..8637)
                     /locus_tag="VVL45_001838"
     CDS             complement(7774..8637)
                     /locus_tag="VVL45_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEU4187971.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTPLYFELNWILLQGLICQRDASYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQEAMHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(8640..10541)
                     /locus_tag="VVL45_001839"
     CDS             complement(8640..10541)
                     /locus_tag="VVL45_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU4187972.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFNDLRRTERQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETIAWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLVTEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKSANMTFQSEKTSGNVVLTVENAAV
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSEQV"
BASE COUNT         3222 a         2370 c         1911 g         3241 t
ORIGIN      
        1 gtttcaatct attataatac caaaaagttc gtattttttc tataattatt attattttat
       61 agcaaaaaaa taaaaccagg aaaacaattt cctggtttta cacgttattt cacatcaaaa
      121 acaatggatt tgacatttgt catcgcttca atgctatatt taattccttg cactccagct
      181 ccagaacctt tgacaccaag gaatgggaaa ttatctggac cacgctgggt tttattatta
      241 atgtggactg tacctacttc aagtttttca gcaatttcaa aggctttttt gaaatcattt
      301 gtaaagactg atgattgaag gccgaattca gattcgttgg caaaggcaat agcttcctct
      361 acactagcca cacgaatgat tggtaaaaca ggaccaaatg gctcttccca tgccactttc
      421 atatcttttg taacttggtc aaaaagcact ggccagagaa gattgccctc acgtttgatt
      481 ggtgtaagag cctgagcttc tttttcttgt gcatcctcaa tcaagcccca aatgaagtcg
      541 gctgaagcat tgtcaataac aggtgtaata tcagcattgt caaatggatc accaactgtt
      601 aatttagaaa cttcttcctg aagcaaagta gctaatttat ctgctacact ttcgagaaca
      661 atgacacgtt taatggccgt gcaacgttgt cctgagtagc taaaggctcc cgcaacaatt
      721 tgcttggcag catgttccaa atctgcatct tctagtacaa gagctgcatc tttcccacca
      781 agttccaaca tgataggacg cataccagct aaacgaccaa tacgttctcc aataggagtt
      841 gaacctgtaa agttgatgaa gttgacttct ttgtgctcaa tgatataatc cccaatttct
      901 gaaccacgac ctgtaatggt gttgaaaaca cctgccggaa tccctgcttc ttcaaatgct
      961 ttagccaaca agagtccaga aatggaacct tgtgttggtg gcttaaacat gaccacattc
     1021 cctgcaatca aggcaggtgc aattttagaa gcagataaat taactggata attaaaggga
     1081 gcaatcgcta gcacgatacc aactggttca cgacggacaa cagccagttt gtttttactt
     1141 gttgcctcaa aaccaccacc ttccattgct tgtccagtga tacggagacc ttcctcagca
     1201 gcataacgaa tcaagtctgc tgtacgcact acttctccaa ttgctgcttt aatccctttt
     1261 gctacttctt tggcaaggat agtaccaatt tcttccttat cgcgttctaa aatagctgct
     1321 gttttatgca aataagccgc acgttcaact gctgataaag ctcgccatgc tggcagggct
     1381 gcacgcgcag cttgcatagc ctcatcagct tcagtctgag tcatggctgg aactgtaccc
     1441 aattcttctt gattgattgg tgaataaatc gtaatttctt gttcagatga tttccatttt
     1501 ccatttacta aattctgata tcttgtcaaa tttttgtcct cctgaaaatg attaagatat
     1561 attttaccaa attttcagaa aatcacaacc ctttttgcaa aatttttgag attattttca
     1621 caaacttgat ttttcaaagt atactcaata aaaattaaaa aaatccacta cgtcaaggcg
     1681 aggctaatgt ggtttgaaga aattttcgaa gagcgtgaat gagtatcatc tatagtaaaa
     1741 taaaaaaact gaacaatttg gttggggaca gccaaaccaa tttctcacaa tgtttcagaa
     1801 acaagggtgt gctattccaa tttcagccta ctataactgt catagattgc tgaaacaaag
     1861 tctaggtaaa agtcttcata ataaaaagac ctcctatcaa gtgttcaaaa actttgatag
     1921 gaggtcttgt tttgtgaaaa tatttatcaa attttctata caagtgagct gttagccagg
     1981 ttctttctat tctttcaatt tcaatgaatg gattttttac taatactcat aactgggaat
     2041 ttgtctgtgt aaaaatagcg agatagatgg tatttataaa acactcaaga cagctagact
     2101 aatatcattt aaaacattat cttcttttga gcgactgttg gttaccaaca tagctaaatt
     2161 tcctgcattt tcaaattgat agggttctga tttagcattc acaaccacca agaggtgttc
     2221 tttgccgtga acttcataga taaggtagcc gctatgttca atcgcagaat gcacaaagac
     2281 atgatggtaa atttcatcat agctagagta agaaaaggca ccagtttttg tcttcaatcg
     2341 gatgacttga cggataaact caatactgtc ttgacgctca ttaatcaagt tccagttcac
     2401 ttggttcaca ctgtcaggag cattatagct attcatcgca cgctctctat catcatgggt
     2461 caactcacca ttttcaccag tcgcaaccag tttggtacga ccaaattctt gaccgatttc
     2521 cataaaggcc atcccctgca tgagcagatt catggctgtg gcagtttcga ccttgcgcat
     2581 gatttgctct gaactttggt ctggatgaag ggttgccaat aaatcgtgaa gattgtaatt
     2641 gtcatgggct tctacatagt taagcacctg atttggatgt gtatagcttc ctaattcacg
     2701 acttcctagg attgctttag ctagaattgg ctctgtcgca gcaccactga caaaacctga
     2761 cttgatagca ccataaactt ctcccccttt gacagcatcg cgctgattgt cattaaagaa
     2821 accaatattt ggcatctggt aggcattgtc cttcttggcc ttatcataag gggcaagacc
     2881 tgttcccata tcccatcctt ctccatagag gataatgttg gagtcgattt catccaagct
     2941 ttgacgaatc atctgcatgg tcttgacatc atgaatcccc atcaagtcaa aacggaagcc
     3001 gtcaatatta tattcctgca cccagtatag aagagaatca atcatatact tgcgaaacat
     3061 ttcgtgttca ctggctgttt catttccaac acccgttcca ttctggaagg taccatctgg
     3121 attcatacga taatagtaat cagggactgt tgtttggaat ggtgcatcaa caactgagaa
     3181 ggtatggtta tagactacat ccataatgac tccaataccc gcatcgtgat aagcttgaac
     3241 catcaccttc aaatcacgaa tgacctgagc tggatcatct ggattagttg aaaaactagt
     3301 ttctggcgcg ttatagtttt gtggatcata accccagttg taggttacat ttccatcctc
     3361 atcgtattct ttatgacggt ctgcaattgg ttgcaattga acataattgt agcccagctt
     3421 cttgatgtaa tcaaaagcag ttgactggcc gtattggtta actgttccag cctgagcagc
     3481 acccaagaaa gttcctcgaa gatgttcatc tacacccgat gtaggtgatt tagtcaaatc
     3541 acgaatatgc atttcacaga taactgcctt acatggattt tccaagcgcc aagtagcctc
     3601 cgaaccgtgc ttaacctcga agttttcaac ttgcttttct acatggctca gaatagctga
     3661 acgtttgcca tcagggctgg tcgcgattgt ataaggatca cgtgtcagtg tttggtgatg
     3721 agggaattgg acttgatact gataagtctt acctaccaaa tcttcttcaa catccaaact
     3781 ccagacaccg attgtattgt ccttatgatt ataagagtag ctattgcctc ttttcatctc
     3841 aaaagtcttc caaacgggtg catcattagc agctgattca taaacgacaa cttgcacttc
     3901 tgtcgctgta ggtgaccaga gggaaaaatg agcctgattg tcctctacac ggcaacccaa
     3961 ttctccttgg taaccccaat gatgatcaaa actagcactg ttaatggcct tatcaaaggc
     4021 aaaaggattt tgatttttat agaaaggact ggcaatagca ggattttcag agtaataaat
     4081 cctatcatcg ccttccaaaa tccagacctc tgttaatagg ggatagtgat taaaacggat
     4141 agaatattct ttactagttt gacctgtatg aaccacaaaa ttcaagcttt ctataacatg
     4201 tgaacttggg tgttcaaagc taaataaagc tccaaaataa tcttctttgt aggttagcaa
     4261 atcaattcgt tgatcctgac tttttacaaa ggagcaagtg tcatattctc cattcttacg
     4321 atggtaatga atgcgcatag ggtagttata cattttttat ttttcctttt tactttgttt
     4381 ctatttcact aataaatttt tgtcaatctc gtctcaatta acagacatag tcatattctc
     4441 taaactctgt ttttaaacga tccattacaa actttctagc catgcctcat ctctgacctg
     4501 gataccaagt tcttgtgctt tttgcagttt acttccagcg tctgcaccta ccacgacgag
     4561 gtcggtcttt ttggaaacac tacctgtcac tttggcaccc agactttcga gtttactttt
     4621 agcttctgag cgcttgagtc gttccaattt tcctgtcaat accacggtca aacctgacaa
     4681 ggccgcatcc gctactaccg tctgtccttt atagtccaga ttgaccccag tttctttcaa
     4741 ttctctgagc agaatttcag agccttctgc cgcaaaataa gtctgaagac ttttggcaat
     4801 cacgccacct agactttcaa tactagccac ttcctctgaa tctgcctgat acagattttc
     4861 aattgaatgg aaatattgaa gtaaaagctg actagccttg cttccgacat gacgaattcc
     4921 caaaccaaat aagagcttct cggcagaatt ttcctttgat gcttggatag cctgatacag
     4981 tttagcagcg gacttttcct taaccccctc taaaaggagg aaatcctctt cttgcaaacg
     5041 ataaatatcc gccacatcct tgactaaatt agcagcaaaa agcttctcaa caatagatgg
     5101 accaaggcct gtaatattca tagcatcacg agaagcaaag tgaatcaagc cttccatgat
     5161 ttgagcaggg caacgcggat tgatacaacg tagggccact tcatcttcaa agtgcaacaa
     5221 gtcagagtta caacttggac agtttgtagg gatatctagt ttttcttcag aaacccgttt
     5281 ggactctacc acacgtaaaa cggcagggat gatgtcacca gccttatata caatgaccgt
     5341 atcgtctttt cggatatctt tttcagcaat ataatctaca ttgtgcaggg tcgcacggct
     5401 aacagtcgta ccggcaagtt gtactggtgt tagattagca gttggagtta caacaccggt
     5461 acggccaact gtccagtcaa ctgataagag ttgagcttct ttttcttcag cagggaactt
     5521 gtaggctact gcccactttg gagccttaac ggtaaaacca agttcttctt gacttgctag
     5581 gtcgttgacc ttgattacca ctccatcaat atcgtaaggc agattttccc gttcctgtcc
     5641 tacttcttgg ataaaattcc agatttcatc tatgttttca gccaagattc gcttaggatt
     5701 gaccacaaaa cctagttgtt ctaggtactt caaacccttt tcttggctat cacgagttga
     5761 agggctggct tcttgataga gaaacgttgc aagattacgc ttggcaacta ctgctgtatc
     5821 caactgacgc agagttcctg ctgccgcatt acgaggatta gcaaattcag gctctccatt
     5881 ttcttggcgc acttggttaa cttggtcaaa ggaagcgcgt ggcatgtaac attccccacg
     5941 aactgtgata tctagttctt ctggcaaagt caaagggatg tccttaacac gcttgaggtt
     6001 ttctgtgata ttttcaccaa ttgaaccatc tccacgtgtt accccagcaa ccaaaatccc
     6061 cttttcataa gtcagcgaga tagataagcc atcgattttc agctcacaaa tataggtcgg
     6121 atgagccact tccttacgaa cacgcgcatc aaaagcatct agctcctcac gtgaaaaagc
     6181 atcctgcaaa ctataaagag gatactgatg actgtatttt tcaaaaccat ctaaaacctt
     6241 gccaccaaca cgatgagtcg gactgtctgc tagcacttgc tctggataag cagtttctaa
     6301 ctcgaccaac tcacggtaaa ggcggtcata ctcactgtct gaaaccgagg gattatcgct
     6361 ggtatagtac tcagtcgcat agcgattgag caaagcgact aactcattca ttcttttatt
     6421 cataagacca ttttaccata aaacaagctc tcctcacaaa cgagaagggc ggaaaaaaca
     6481 cttagtttga aattattttt gaaactcaag caaccttata tcaatttttc aaaatgagtt
     6541 cgaacataaa taaacgatat acaagacaag atgataacac cacttccaat tatcaggaaa
     6601 gaagagagat gtacacttgg caagactgtc ataaatcctt ttgcaatagg cataaataga
     6661 atagctaagg taaaaattgt actcagtact cttccaagaa attcgctctc aaccttggtt
     6721 tgtacttgag taaaaaagtg aatattaaaa atcgtcataa acaattcaca aactaaattt
     6781 ccagaaaagg aaagaaaagt tggaagtggt aatcccatca taaaaactcc gacacctgtc
     6841 aaagccagta aaatcaaaag attataaata ttagctttaa ttttactagc tagaagagcc
     6901 ccaatgatgg aaccaatagc ccccatagtt aaaatacttg cataggctcc ttctgacccg
     6961 taaagctgat tcgaaaaggg aagtagaaat tcaaaagctg caaaaaagaa attaacgctg
     7021 gaagctacca gcaaaaggaa gaaaatttct tgctgatgcc agatatagtg taacccatcc
     7081 ttgatatcta caaaaatatc tctcccagta aaagcctttt tctcttgaac ttttgcttcc
     7141 tcttttggaa ggaaagccac tagaacaaaa gcaatgaaaa aagtcagcga gtctagcagt
     7201 agcgtcatat ggagacttgc aaactgtaaa acaaggaagg aaagaacagg agagctaaca
     7261 cctacaacct gcaaaaccag ctctaagcga gaattataga tcacaatctc atctttctcc
     7321 accacttcag ttatgatagc tttattggct gtgcgagaaa aggcaaaagc aatagcctgc
     7381 acaatgttag caacaatcaa agcgccaatc atccagctat cattccttat gaaagaaata
     7441 gccagacaaa gaatcccaca aacaagatct gccgtcatta aaatcttacg acgagaaaaa
     7501 cggtctgaaa taactccgcc aaagggattg acgagaatag atgtgacgag ctcagaaatc
     7561 tgatacattc ctaaaactgt ctgtcctata gtccccatag aagccaacca gacactattt
     7621 ccataatcat agagcatatt tcccatttta ttgatagccc cacggctaat caactgcact
     7681 gcatagcgat tcatattaaa gctcctctca aattttgaaa ctattgtatc aaaaccgaaa
     7741 ggagcttttt attttttccc ttatttggga aaattaactt ttgacaaatt tttcgtagtg
     7801 ttcctgataa taggctactt gctctggaag acctaacaca tcaaaaatat gcatggcctc
     7861 ttgcatctgc ttacagcctt ctttacactg tcctttttga tataaggcaa aaccttttaa
     7921 ataatggaaa acattacgct cataaagctt aatacctttg tcaataatct tctctgtata
     7981 agcctcaaaa tagttggcat tataaaaaga agaatgctct aaacaatgct ggtaacaatt
     8041 gagggccaaa atcaacacta atctcttatg gcgactaatc tcttggtaaa attcctccct
     8101 ctccataact tctctaccaa tccgagtgac atagtctaca tcgtagaaac tatagaggtt
     8161 accgaaaaga atcaactcat acatggtcca ttcttctgtt ttgaagagat aatctgctac
     8221 cttatccaaa tcatcctgct tcatatcata actcgcatct ctttgacaaa tcagaccttg
     8281 tagcaaaatc cagttcagct caaaataaag gggagtcgtc gaactcttag acttttcaag
     8341 ttgttctctt tgaagctttt gaaaacctgc aatatcgttt gaatagtaaa gtgggataat
     8401 ctgtgccatc atagacacat gttcatgatt atgaaaattc cttgccttat ccatgaaatt
     8461 ttcgattgtt acatgaatgt tatccaaaat ctcaaagaaa cgggagactg ccaggtcaga
     8521 ctccccaagc tcaaagcgag ataactgaga ggtagagcag gattcgcctg ctgcttcctt
     8581 taaagaataa tttccacttg ttcgaaattc acgaaatact tttccaagat gttccatctt
     8641 tacacctgct ctgataattc ttcccactca agcatagctt cttcctgacg atggctgatt
     8701 ttgtccagct cagcctgtaa ttccatgagt ttgtcggcat cgtttgtttc caacatttgt
     8761 tcagaaatgg cttggctttg actttctagc tcttcaattt cagcttctag actttcgatt
     8821 tgtcgcatga gtttgcgaac ttctttttga ctttctttct gggcctgata gtcattgact
     8881 ggacttgctt cctttgcttg attgctagtt gaagcttcct cagtctgact catttctgct
     8941 gttgctttct tctcaacata gtagtcgtaa tctccaaggt agagagttga accattctca
     9001 gacaattcca aaacatgagt tgccacacga ttgataaagt aacgatcatg actgacaaac
     9061 agcaaggttc catcaaagtc aatcaaggca ttttctagca cttccttact atcaatatcc
     9121 aagtggttgg tcggctcatc cagaatcaaa aagttattgt tttccataga caatttagct
     9181 aaaagcaaac gagctttttc gccaccagat agcatgccga ctgatttttt aacatcatct
     9241 cctgagaaaa ggaaggctcc aagacggttg cggatttcaa cttctggtgt cagtttgaaa
     9301 tcattccaga gttcatccag caccgtatta cttggtgtca gcttgctttg ggtttggtca
     9361 tagtaaccaa cctcaacatt agcgccaaag cgcttttctc ccttgataaa aggaatctgg
     9421 tccacaatag acttgataaa ggttgacttg ccgataccat ttggaccaac gatagcgaca
     9481 gcattcatct tacgaagatc taggttaatc ggttgtgaca agacttcccc gtcatagcca
     9541 acagctgcat tttcaacagt caaaacaaca ttgcccgacg ttttttcaga ctggaaggtc
     9601 atgttggctg atttcttgcc agcttcaggc ttgtccaaac gttccatttt ttccagttgt
     9661 ttacggcgag attgagcacg tttagtcgtt gaagcacgaa ctagattgcg attgacaaag
     9721 tcttccagag cagcgatttc cttctgttgc ttttcatagt tttttgcctc agtaactagc
     9781 ttttgctcct tcaattcgac aaaacgagag taattcccca catagcgatc caaggaatgc
     9841 ttggtcaaat ctagcgtaat tgtcgcaacc ttgtccaaga aataacggtc gtggctgacg
     9901 ataatgaggg caccgctata gtttaccaag taattctcta gccaggcgat ggtttcaata
     9961 tccaagtggt tagttggctc gtccaagacc aagagattgg gcttttcaag gagcattttg
    10021 gcaagtgcca aacgagtatt ttgaccacca gaaagctcag caattttcat ctgccacata
    10081 gactcgtcaa acttgaatcc attcaaaatc gctcgaatat cagcttcata ggtaaagcca
    10141 cctgcttggc gaaaattctc agataagcgg tcataatctg acatcagttt atccaaatcc
    10201 tcaccagact tttcacccat ctccagctcc atctgacgca gttgtctctc cgtccgacgc
    10261 aaatcattaa agacatgaag catttcatcg tagatggtat tttcagactc aaaacggcta
    10321 tcttgggcta ggtaagacag agaaatatct tttttcttat tgatttctcc gctagttggc
    10381 tcctcttctc caactaaaat cttcaaaaga gtagacttac ctgcaccatt tttcccaaca
    10441 agagcaatcc gatctcgttc atcaacctgc aggttgatat tatcgaaaag aacctctcct
    10501 gcaaaagaac gttcaatttt attagcttgt aaaataatca tacaagtagt atagcatgtt
    10561 tccctaaggc attcaagata atcgtaagtc ttttagtaca acttttataa cataaaataa
    10621 actaaattat gtatatttta tattagatta cttcactatc ttgttggatt ttctaaccag
    10681 ctaatcttgt ttcaaatagt tatcgcacaa gtctattatt taattctttt catcatttac
    10741 gtac
//
LOCUS       DAVHEX010000068        10354 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVHEX010000068 DAVHEX010000000
VERSION     DAVHEX010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10354
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.069"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..455
                     /locus_tag="VVL45_001840"
     CDS             <1..455
                     /locus_tag="VVL45_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU4187973.1"
                     /translation="KNLVFCLEADNQEQLFDQVASLLEEREIVTPTYREALITREKSF
                     PTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDKEVEVSLLF
                     FIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEKLYAYIAEATA"
     gene            493..795
                     /locus_tag="VVL45_001841"
     CDS             493..795
                     /locus_tag="VVL45_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU4187974.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSAPVFDNIRLPAKQNMV"
     gene            803..1607
                     /locus_tag="VVL45_001842"
     CDS             join(803..1171,1173..1607)
                     /locus_tag="VVL45_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEU4187975.1"
                     /translation="MNDEASKQLTDARFKRLVGVQRTTFEEMLAVLKTAYQLKHAKGG
                     RKPKLSLEDLLMATLQYVREYRTYEEIAADFGIHESNLLRRSQWVEVTLVQSGVTISR
                     TPLSSEDTVMIDATEVQINRPKKELANDSGKKKFHAMKAQEIVTSQGRIVSLDITVNY
                     CHDMKLFKMSRRNIGQAGKILADSGYQGLMKIYPQAQTPRKSSKLKPLIAEDKAYNHA
                     LSKDRSKVENIFAKVKTFKMFSTTYRNHRKRFGLRMNLSAGTINHELGF"
     gene            1656..2120
                     /locus_tag="VVL45_001843"
     CDS             1656..2120
                     /locus_tag="VVL45_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HEU4187976.1"
                     /translation="MGGHPGFNCLLLDDEVYEDYYLEFEKEEICSVPRPFPETGMLDF
                     QDRSPWLEGQKEIDLSYDLFSTDAVTLDELQSRTIALRSLKHDKGLKVHFAEFPNLII
                     WSTLNKGPFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            2277..2702
                     /gene="lacA"
                     /locus_tag="VVL45_001844"
     CDS             2277..2702
                     /gene="lacA"
                     /locus_tag="VVL45_001844"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEU4187977.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            2733..3248
                     /gene="lacB"
                     /locus_tag="VVL45_001845"
     CDS             2733..3248
                     /gene="lacB"
                     /locus_tag="VVL45_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEU4187978.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPSEENKKLIAKIEHLESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            3259..4188
                     /locus_tag="VVL45_001846"
     CDS             3259..4188
                     /locus_tag="VVL45_001846"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEU4187979.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLTKA
                     NVLGMLNAQEKMTGHVNMANYQVLYDQLIVKEV"
     gene            4190..5170
                     /gene="lacD"
                     /locus_tag="VVL45_001847"
     CDS             4190..5170
                     /gene="lacD"
                     /locus_tag="VVL45_001847"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEU4187980.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            5404..6240
                     /locus_tag="VVL45_001848"
     CDS             5404..6240
                     /locus_tag="VVL45_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU4187981.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENQ
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            6276..6593
                     /locus_tag="VVL45_001849"
     CDS             6276..6593
                     /locus_tag="VVL45_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU4187982.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            6593..8284
                     /locus_tag="VVL45_001850"
     CDS             6593..8284
                     /locus_tag="VVL45_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU4187983.1"
                     /translation="MNKLIAFIEKGKPFFEKLSRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAFVPNSWGFKWSDEVVAFLMKPYSYSMGILALLVAGTTAKSLTDSVNRSMEKTNQ
                     INYMSTLLAAIVGLLMLAADPIESGLATGFLGTKGLLSAFLAAFVTVAIYKVCVKNNV
                     TIRMPDEVPPNISQVFKDVIPFTLSVVSLYALDLLARHFVGASVAESIGKFFAPLFSA
                     ADGYLGITIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAEVNLNLLQQGMHADKILT
                     SGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNRAIGRASVVPTFFGVNEPILFGAPLV
                     LNPIFFIPFIFAPIANVWIFKFFIETLGMNSFTANLPWTTPAPLGLVLGTNFQVLSFI
                     LAALLIVVDVVIYYPFLKVYDEQILEEERSGKSNDELKEKVAANFNTAKADAILEKAG
                     VEAAQNTITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLPE
                     FDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQEQFD"
     gene            8499..9905
                     /gene="lacG"
                     /locus_tag="VVL45_001851"
     CDS             8499..9905
                     /gene="lacG"
                     /locus_tag="VVL45_001851"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEU4187984.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDPENPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILLVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     VAPDYVPRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            9995..10207
                     /locus_tag="VVL45_001852"
     CDS             9995..10207
                     /locus_tag="VVL45_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEU4187985.1"
                     /translation="MNFEKCSQIPCLTSEELKSLGKWYVSTGKEWICHSDDELEEFKN
                     LFLNFINPEEWDTISFDSDFMPFQQS"
BASE COUNT         3298 a         1860 c         2131 g         3065 t
ORIGIN      
        1 acaaaaacct tgtgttttgc ttagaagcgg ataatcaaga acaactcttt gatcaggtag
       61 caagcttgtt ggaagaacga gaaattgtca ctccaactta tcgtgaagcc ttgatcacgc
      121 gtgaaaagtc atttccaact ggtttagata tggaatttct aggaaaggac ttgccaaatg
      181 tagcgattcc tcacacagat attgttcata atctagctga aaaagtggtg gttgttcgat
      241 tagaaaaacc agtaactttt cacaatatga tagctcctga taaggaagta gaagtatccc
      301 tactcttctt tatcattaac aattcaagtt caagtcaaac aaatattctg gctcagttga
      361 tggacttttt cacaggaaat ggacatcttg aagatttatc aaaaatttcc gaaccagaaa
      421 aactttatgc ttacattgct gaagcaaccg cttaatcttg tctattaaaa aataaaatcg
      481 gaggaaatct aaatgattaa aattcttgct gcctgcggtg caggtgttaa ctcaagtcac
      541 caaattaaaa gtgctctaga agaagaactt tcaaaccgtg gttatgatgt tcactgtgat
      601 gcagtcatgg tgaaagatgt aaacgaagac cttatgaaag gttacgatat ctttacacca
      661 atcgctgcaa cagaccttgg ttttgaacca ggtatcccag ttatcgaagc tgggccaatc
      721 ttattccgta taccagcaat gagcgctcca gtatttgaca atattagact ccctgcgaaa
      781 caaaatatgg tatagtagtt ctatgaatga tgaagcaagt aaacaactaa ctgatgcacg
      841 atttaagcgt cttgttggtg ttcagcgtac cacttttgaa gagatgttag ctgtattaaa
      901 aacagcttat caacttaaac acgcaaaagg tggacgaaaa cctaaattaa gcctagaaga
      961 ccttcttatg gccactcttc aatatgtgcg agaatatcga acttatgaag aaattgcggc
     1021 tgattttggt atccacgaaa gcaacttact ccgtcggagc caatgggttg aagtaactct
     1081 tgttcaaagt ggtgttacga tttcaagaac tcctctcagt tctgaggaca cggtaatgat
     1141 tgatgcgacg gaagtacaaa tcaatcgccc taaaaaaaga attagcgaat gattctggta
     1201 aaaagaaatt tcacgctatg aaggctcaag agattgtcac aagtcaaggg agaattgttt
     1261 ctttggatat cactgtgaac tattgtcatg atatgaagtt gttcaaaatg agtcgcagaa
     1321 atatcggaca agctggtaaa atcttggctg acagtggtta tcaagggctc atgaagatat
     1381 atcctcaagc acaaactcca cgtaaatcca gcaaactcaa gccgctaata gctgaagata
     1441 aagcctataa ccatgcgcta tctaaggata gaagcaaggt tgagaacatc tttgccaaag
     1501 taaaaacgtt taaaatgttt tcaacaacct atcgaaatca tcgtaaacgc ttcggattac
     1561 gaatgaattt gagtgctggt actatcaatc atgaactagg attctagttt tgcaggaagt
     1621 ctaataaaga aactagcaag gtcaggcctt actttattgg tggacatcca ggatttaatt
     1681 gtcttctact tgatgatgaa gtctatgaag attattatct agagtttgag aaagaagaga
     1741 tttgctctgt tccacgtcct ttcccagaaa caggtatgtt ggatttccaa gatagaagtc
     1801 catggctaga gggacaaaaa gaaatagatc ttagttatga tcttttcagc acagatgcag
     1861 tgactttgga tgaattgcaa tctcgaacaa ttgcccttcg ttctcttaaa catgataagg
     1921 gattgaaagt gcattttgca gaatttccaa acctcatcat ctggtcaact ttgaacaagg
     1981 gacctttcat tacctttgaa ccatggtctg gcttgtcaac attccttgaa gaaggagatc
     2041 atttagaaga taagaagaat gtttgtctct tagaagccaa tcaggttgaa gaattagggt
     2101 ttgagataga agttttataa aaaagtaaaa aataaataac aaaaacaaac acaaactgtt
     2161 gacttcttca aacaatagta gtatattatg tttgtgagga ctaattgact gttttttaaa
     2221 gacagccaat acactgctgg aatccagcat agaaaaaata aaaaggagta tacaatatgt
     2281 ctattgttat cggtgcagat gctgcaggtt tgagattgaa agaagttgtg aaagacttct
     2341 tagaaaaaga aaacttccac cttgtggatg ttacagctga aggtcaagac tttgttgatg
     2401 tgactctcgc tgttgctgca gaagtaaaca aagaagaaca aaaccttggt atcgtgattg
     2461 atgcttatgg agctggtcca tttatggttg caactaagat caaaggaatg gttgctgcag
     2521 aagtatctga cgaacgttca gcttatatga ctcgtggcca caacaactca cgtatgatca
     2581 ctatgggtgc acaacttgtt ggtgatgaat tggctaaaaa catcgctaaa ggatttgtta
     2641 acggtaaata cgatggtgga cgtcatcaaa tccgggtcga catgttgaac aaaatgggct
     2701 aattagatta cagtaagaaa ggtaagttaa aaatgagaat tgcaattgga tgcgaccaca
     2761 tcgtaacaga tgaaaaaatg gcggtttcag aatttttgaa atcaaaagga tatgaagtca
     2821 ttgactttgg tacatatgac catacacgga ctcactaccc aatctttggt aaaaaagtag
     2881 gggaagctgt aactagcggc caagctgatc ttggggtatg tatctgtggt actggtgttg
     2941 gtatcaacaa cgctgtaaat aaagttccag gtgttcgttc tgccttggtt cgtgatatga
     3001 caacagccct ttatgctaaa gaacaattga acgctaacgt tatcggcttt ggtggtaaga
     3061 ttactggtga attgcttatg tgtgatatca tcgaagcttt cattcatgct gaatacaaac
     3121 catctgaaga aaacaagaaa ttgattgcga aaattgaaca ccttgaaagt cacaatgctc
     3181 aacaaacaga cgcaaacttc tttacagaat tccttgaaaa atgggatcgc ggagaatacc
     3241 acgactaaga ggtgacctat gattttaaca gtcacaatga acccatccat tgatatttcc
     3301 tatcccttgg atgagttgaa gattgatact gtcaatcgtg tggtggatgt aaccaaaacg
     3361 gctggtggta agggactcaa tgttacccga gtactttcag aatttggcga ttctgttctt
     3421 gctactggtt tagtgggtgg caaacttggt gagtttttgg ttgaacatat cgataatcaa
     3481 gtaaagaaag atttcttctc aattcaggga gaaactcgta actgtatcgc tattctccac
     3541 ggagacaacc aaacagaagt tcttgaaaaa ggtcctgaag tattggaaca ggaaggtcaa
     3601 gactttttgg aacatttcaa aaaactcttg gagtcagttg aagtagtggc catctcaggt
     3661 agtctgccag ctggccttcc agttgattac tatgcgagct tggtagaact tgctaatcaa
     3721 gctggcaagc ctgtagtctt ggactgctca ggtgcagcac ttcaggctgt tcttgaatca
     3781 ccccataaac caacagtcat caaaccaaat aatgaagaat tgtctcagct tcttggaaga
     3841 gaagtttctg aggatttgga tgaattaaaa gaagtacttc aagaaccttt gtttgcaggg
     3901 attgaatgga ttatcgtttc acttggtgcc aacggtactt ttgccaaaca tggtgacact
     3961 ttctacaagg tagatattcc tagaattcag gtggtaaatc ctgttggatc tggagactct
     4021 actgtggcag gaatttcttc aggacttctt cacaaagaat cggatgcaga attactcacc
     4081 aaggcaaatg tccttggtat gctcaatgct caagaaaaaa tgactggtca tgtcaacatg
     4141 gccaactatc aagttctata tgatcaatta atagtaaaag aggtataaaa tggctttaac
     4201 agaacaaaaa cgtgtacgct tagaaaaact ttctgatgaa aatggtatca tctcagctct
     4261 tgcatttgac caacgtggtg ctttgaaacg cctcatggct caacaccaaa cagaagaacc
     4321 aactgtggcc caaatggaag aacttaaagt cttggtagca gatgaattga ctaaatacgc
     4381 ttcatctatg cttcttgacc ctgagtatgg acttccagca actaaagctc ttgatgaaaa
     4441 agctggtctt ctccttgctt atgaaaaaac aggttatgac acaacaagca caaaacgctt
     4501 gccagactgc ttggatgtct ggtctgcaaa acgtattaaa gaagaaggtg cagatgcagt
     4561 taaattcttg ctttactatg atgtagatag ctcagacgaa ctcaatcaag aaaaacaagc
     4621 ctacatcgaa cgcatcggtt ctgagtgtgt ggctgaagat atcccattct tccttgaaat
     4681 tcttgcttac gatgaaaaaa ttgcggatgc aggttctgta gaatacgcga aagtaaaacc
     4741 acacaaagtt atcggcgcta tgaaagtctt ttcagaccca cgctttaaca ttgatgtctt
     4801 gaaagttgaa gttcctgtta acattaaata tgttgaaggc ttcgctgaag gtgaagtagt
     4861 ttatacacgt gaagaagcag cagccttctt caaagcgcaa gatgaagcaa cgaacttgcc
     4921 atacatctac ttgagtgctg gtgtatcagc taaactcttc caagatactc ttgtatttgc
     4981 tcatgaatca ggtgcaaact ttaacggagt tctttgtggc cgtgctacat gggcagggtc
     5041 agttgaagct tacatcaaag atggtgaagc agcagctcgc gaatggcttc gcacaactgg
     5101 atttgaaaac attgatgagc tcaataaagt tcttcaaaca acagcgactt catggaaaga
     5161 acgtgtgtaa gaaagtcctc ctagtttagg aacatgaatc taaaaaaatt taaaaaaagt
     5221 tgtatgtaaa ggtttacaaa ataacttact tgtgctatac ttaaatcaca agttaataca
     5281 aggtgagtgt tactaagtaa tattaggcat gatcacaggt gaattagaaa tcagctgatt
     5341 ttctagttca tttgtggtca ttttttgtac ttatatacct ttaagatata aaaggaggtt
     5401 gacatgtatc gaattctaaa tccaatgaat cacaatgtct cgcttgtcag aaatgataag
     5461 ggagaagagg tgattgtaat tggtaagggg attgcattcg gaaagaagaa gggggatttg
     5521 attgctgaaa atcaggttga gaaaatcttt cggatgaaga ccgaagagtc cagagaaaac
     5581 tttatggctc ttctcaaaga tgttccgctt gattttatca cagtgaccta tgaaatcatt
     5641 gataagctat caaagaaata tcattatccg attcaagagt atctctatgt aaccttgaca
     5701 gatcatattt actgttctta tcaagctcta actcaaggaa ggtacaagga tagtaatctg
     5761 ccagatattt ccgctaagta tcctgtcgct tttcaaatcg caaatgaagc ttttgaaatt
     5821 taccgtcaga agctagcaga tcattttcct gaggacgaaa ttattcggat tgcttatcat
     5881 ttcattaatg ctgaaggtga aaatgaagtg gaacttgtgg agtcgattga taagaggaaa
     5941 gaaattctca ggaatgttga agaagtttta acggactatg caattcaacg aactaaaaag
     6001 aataaccatt tctatgatcg ctttatgatc catttgaatt atttcttgga ttatttagac
     6061 agatctagag atgataacca atcacttctg gatatggaag atcatattaa acaatcctat
     6121 ccaaaagcct tcgagattgg ttccaagatc tatgatgtga ttacgcaaca tacgggtctt
     6181 gatttgtata aaagtgaacg agtttatcta gttctacata tccaacgttt attgtcataa
     6241 aaatttattt aaaactatat aaggagaatt ctatcatgaa tagagaagaa gtaacattgt
     6301 taggttttga aatcgtagcc tatgctggcg atgctcgttc aaaactattg gaagccttga
     6361 aggctgctga agctggtgat tttgaaaaag cggacgctct ggtagaggaa gctggtagct
     6421 gtattgcaga ggctcaccac gcgcaaacaa gtctattgac taaggaagct tcaggtgagg
     6481 acttggctta tagtgtaacc atgatgcatg gccaagacca cttaatgaca actatcttgt
     6541 taaaagattt gatgcatcat ttaattgaac tctacaagag aggagttaaa taatgaataa
     6601 actaattgca tttatcgaga aaggaaagcc tttctttgaa aaactatctc gtaatatcta
     6661 tcttcgtgct attcgtgatg gtttcattgc aggtatgcct gttattctct tctcaagtat
     6721 ctttatcttg attgcctttg taccaaactc atggggcttt aaatggtctg atgaagttgt
     6781 agcctttctg atgaaacctt atagctattc tatgggtatt ctggctctct tggtagctgg
     6841 tacaacagct aagtcattga ctgactcagt aaaccgtagc atggaaaaaa ccaatcaaat
     6901 caactatatg tcaacattgt tggcagcaat tgttggtttg ttgatgttgg cagctgatcc
     6961 tatcgaaagt ggtctagcta ctggattctt ggggacaaaa ggtttgcttt cagccttcct
     7021 tgctgccttc gttactgtag ccatctataa ggtttgtgtt aagaacaacg tcactattcg
     7081 tatgcctgac gaagttccac caaatatctc acaagtcttt aaagatgtga ttccattcac
     7141 tctctcagtt gtttcacttt atgctcttga tttactagct cgtcattttg ttggtgcaag
     7201 cgtagctgaa tcaatcggta aattctttgc accactcttc tcagcagcag acggatacct
     7261 tggtattacc attatctttg gtgcctttgc cttcttctgg tttgtgggta ttcatggtcc
     7321 atctattgtt gaacctgcta tcgcagcaat tacctatgca aatgctgaag tcaacttgaa
     7381 ccttctccaa caaggtatgc acgctgacaa gattcttact tctggtacac aaatgtttat
     7441 cgttaccatg ggtggtactg gtgcgacact ggttgttcca ttcatgttca tgtggttaac
     7501 aaaatcaaaa cgtaaccgtg caatcggacg tgcttcagta gttcctacct tcttcggtgt
     7561 aaatgagcca atcttgtttg gtgcacctct tgttttgaat ccaatcttct tcattccatt
     7621 tatctttgct ccaattgcaa acgtatggat ctttaaattc tttattgaaa ctcttggaat
     7681 gaactcattc actgctaatc taccatggac aaccccagct ccactaggtc tagttcttgg
     7741 tactaacttc caagttctat cattcattct tgctgccctt ctaatcgtgg ttgacgttgt
     7801 catttactat ccattcctta aggtctatga tgaacaaatt cttgaagaag aacgttcagg
     7861 taagtctaat gatgaattga aagaaaaagt tgctgcaaac ttcaacactg caaaagcgga
     7921 tgctattctt gaaaaagcag gtgtagaagc agcacaaaat acaatcacta aagaaacaaa
     7981 tgtcctcgtt ctttgtgcag gtggaggaac aagtggtctc cttgcaaatg ctctaaacaa
     8041 ggcagcagca gaatacaatg tccctgtgaa agcagcagca ggtggctatg gtgctcaccg
     8101 tgaaatgttg ccagagtttg atttggttat ccttgctcct caagttgctt caaactttga
     8161 agatatgaag gcagaaacag ataagctcgg tattaaactt gctaagacag aaggcgctca
     8221 atacatcaaa ttaactcgtg atggaaaagg tgccctagca ttcgtacaag agcaattcga
     8281 ttaaggctag gaactgtgaa acagtctccc atcgttacgg aaatcgctat ggcgaatttc
     8341 ctaatcgcct tattaattcg tcggtaaaaa gatatcgttt ttacctcctc atgtcacaat
     8401 tcggtgactt ggtacaagaa gtgagatgga gaaggatggc tcactgactc ctctcctctc
     8461 acttttactt tatttaaatc aagaaatagg tgaaaaaaat gacaaaaaca cttccaaaag
     8521 actttatttt tggtggcgca acagctgcct atcaagcaga aggtgctaca catactgatg
     8581 gtaaaggacc agtagcgtgg gataaatatc ttgaagataa ctactggtac actgcagaac
     8641 cagccagtga tttttacaat cgatatccag ttgacctcaa gctagcagaa gagtatggtg
     8701 tcaatggcat tcgaatttct attgcctggt cacgtatttt cccgactggt tatggtcaag
     8761 taaatgctaa aggtgttgag ttttatcata atttatttgc agagtgtcac aaacgtcatg
     8821 ttgagccctt tgtaactctt catcactttg acacgccaga agctctccac tcaaatggag
     8881 acttcttaaa ccgtgaaaat atcgaacatt ttgtagacta cgctgccttc tgttttgaag
     8941 aatttccaga agtaaactat tggacaacct ttaatgaaat tggaccaatc ggtgatggtc
     9001 aatatttggt tgggaaattc cctccaggta tccagtacga ccttgccaaa gtctttcaat
     9061 cacaccacaa tatgatggtg tctcatgcac gcgcggtaaa attgtacaaa gagaaaggct
     9121 ataaagggga aattggtgtt gttcacgccc tgcctactaa atatcctcta gatcctgaaa
     9181 atccagcaga tgttcgtgca gcagagttgg aagatatcat ccacaataaa ttcatcttag
     9241 acgcaactta tctaggtcgc tattcagctg aaaccatgga aggtgtcaac catatcttat
     9301 tagtcaatgg tggtagtttg gatcttcgtg aagaagattt tacagcatta gaagctgcaa
     9361 aagacttgaa tgatttccta ggaatcaact actatatgag tgactggatg gaagcctttg
     9421 atggagaaac tgaaattatc cataatggta aaggtgaaaa aggaagctct aagtatcaaa
     9481 tcaaaggtgt tggtcgtcgt gtagctcctg actatgtacc acgcacggat tgggattgga
     9541 ttatctaccc tcaaggtttg tatgaccaaa tcatgcgtgt gaagaaagat tatcctaact
     9601 acaagaagat ttacatcact gaaaatggtc tcggctataa agatgagttc gttgataaca
     9661 ctgtttacga tgatggtcgt attgattacg tgaagcaaca cttggaggtt ttatctgatg
     9721 cgattgcaga tggagctaat gtaaaaggtt acttcatttg gtcattaatg gatgtcttct
     9781 catggtcaaa cggttatgag aaacgttatg gtctcttcta cgtagatttt gaaactcaag
     9841 aacgttatcc taagaaatca gctcactggt acaagaaagt agcggaaact cagattatag
     9901 actagtagaa ttagtcatta gatatagaat tttagtgagt caaaaagatg ttcaaagatt
     9961 ttatccaatc tatttatgaa aaaaagttta tattataaat ttcgaaaaat gctctcaaat
    10021 accgtgtttg acgagtgaag aattgaaaag tcttggaaaa tggtatgtct cgactggtaa
    10081 agaatggatt tgtcattcag atgatgagct ggaagaattt aaaaatctat ttttaaattt
    10141 tatcaatcct gaagaatggg atactatctc ctttgattca gattttatgc cgtttcaaca
    10201 atcgtaacca atttctcaaa aaagttaaat cttatattta gtactctgta aaactcttat
    10261 ctaatcacgt tgcttatact caatgaaaat caaagagcaa ctttaaacta ggaagcgagt
    10321 cgcagatttc tcaatgcata gctttgagga attg
//
LOCUS       DAVHEX010000069        10103 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVHEX010000069 DAVHEX010000000
VERSION     DAVHEX010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10103
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.070"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(<1..608)
                     /locus_tag="VVL45_001853"
     CDS             complement(<1..608)
                     /locus_tag="VVL45_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEU4187986.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLTLVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYESFFGYLCLKSR"
     gene            complement(598..1371)
                     /locus_tag="VVL45_001854"
     CDS             complement(598..1371)
                     /locus_tag="VVL45_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEU4187987.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKENPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            complement(1382..2119)
                     /locus_tag="VVL45_001855"
     CDS             complement(1382..2119)
                     /locus_tag="VVL45_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEU4187988.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEDYNLVWIIAEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAGQ
                     ELIHMMATFVLMDRDSRKVHAVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKV"
     gene            complement(2124..3254)
                     /locus_tag="VVL45_001856"
     CDS             complement(2124..3254)
                     /locus_tag="VVL45_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEU4187989.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENMAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQIEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            complement(3338..3727)
                     /locus_tag="VVL45_001857"
     CDS             complement(3338..3727)
                     /locus_tag="VVL45_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU4187990.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            complement(3743..4126)
                     /locus_tag="VVL45_001858"
     CDS             complement(3743..4126)
                     /locus_tag="VVL45_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEU4187991.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            complement(4127..4915)
                     /locus_tag="VVL45_001859"
     CDS             complement(4127..4915)
                     /locus_tag="VVL45_001859"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEU4187992.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGLRVSQWLSVVFIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            complement(4908..5843)
                     /locus_tag="VVL45_001860"
     CDS             complement(4908..5843)
                     /locus_tag="VVL45_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEU4187993.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            complement(5973..6149)
                     /locus_tag="VVL45_001861"
     CDS             complement(5973..6149)
                     /locus_tag="VVL45_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEU4187994.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            complement(6289..6996)
                     /locus_tag="VVL45_001862"
     CDS             complement(6289..6996)
                     /locus_tag="VVL45_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEU4187995.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            7147..8175
                     /gene="queA"
                     /locus_tag="VVL45_001863"
     CDS             7147..8175
                     /gene="queA"
                     /locus_tag="VVL45_001863"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEU4187996.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKKNGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFS
                     FGDAMFIY"
     gene            complement(8836..9225)
                     /locus_tag="VVL45_001864"
     CDS             complement(8836..9225)
                     /locus_tag="VVL45_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU4187997.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTDWKQENGMWYFYNTDGSMATGWVQVNSSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNASGELAVNTSIDGYRVNDNGEWVR"
     gene            complement(9279..9971)
                     /locus_tag="VVL45_001865"
     CDS             complement(9279..9971)
                     /locus_tag="VVL45_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU4187998.1"
                     /translation="MNTSFVHAADGIQYVRDDTRDKEEGIEYDDADNGDIIVKVATKP
                     KVVTKKISSTRIRYEKDETKDRSENPVTIDGEDGYVTTTRTYDVNPETGYVTEQVTVD
                     RKEATDTVIKVPAKSKVEEVLVPFATKYEADNDLSAGQEQEITLGKNGKTVTTITYNV
                     DGKSGQVTESTLSQKKDSQTRVVKKRTKPQVLVQEIPIETEYLDGPTLDKSQEVEEVG
                     EIGKLLLLQSIL"
BASE COUNT         2952 a         2302 c         1831 g         3018 t
ORIGIN      
        1 cgacttttca gacacagata gccaaagaag ctttcataga gggcaaaaaa gaggaggaag
       61 gcatgaagaa agaaggtctc tggcaaaatc ataataacag gatccttggc tggatcaaaa
      121 agccaggtat catctcccac aaagagaatt tgatggaaaa gagtaaagaa ttggtcaaaa
      181 ccaatcaaaa ctcccccaag tccaatcatc acaggtaaga ctactagagt caggagactt
      241 tttcgataaa gagacaaaaa gtcctttttc acaatcctat tgacaaagac atagaaactt
      301 ggcagtgtca ctagagctac tagctgaacc aaatgaaaga gattcttgac cactgcgaaa
      361 tggtgcagac cagctgctga cgaacgaaaa tcaggcatct gtaagacctg actaaaagga
      421 ttggtcagat aattcatcaa gatatgaaaa ttgtattgaa tggtttctgg ttttagatag
      481 actcgattcg ttaagtttag ccactgaatc tccataggat agaaaatcca agccagataa
      541 atggtcagaa ggattgagag ggagaggaga aagagcatag agccccaaaa ggtcaattta
      601 gttttcatca aaatcccact ccgcaaggct agaaaccaca tgtgtcggtg cgattggcag
      661 gccagctact tcttctgcct tggtaaaacc tgtcgtcacc aagagtgttg gaatgccatt
      721 gtcaatccca gcccggatat cagtcaaata attgtcccca accatgatta actcttcacg
      781 ttccaaacct aagtgctcaa ccgccttgtc cataatgatg gcatttggtt ttccgatata
      841 aaccggcttc actcgtgtcg ctacttcaag cagcgtaatc agtgagccag cacctggcaa
      901 aagaccacgt tccgtcggga tgttgaggtc aggattggtt ccgataaaat gggcaccctt
      961 ttgaatagca agagttgctg tggcaaattt ttcatagtcg acttgccaat ccagaccaac
     1021 taccacgtag gcagggtttt ccttgtcttc cacataacca gccgccttga tggcttcctt
     1081 gagtcctgct tctccgacga catagacggt cttttcaagc cccaaatcat tcatatagtc
     1141 gatggttgcc aaagtcgctg tgtagacagt cgatagaggc gtatcgatat taaaattctg
     1201 agccaacatc tccttaacac tctctggagt gcgggttgta ttgttggtta caaagagata
     1261 gggaatgtcc cgcttttgca attcatgaac aaaagtctct ccagcaggga ttcggtcttt
     1321 ccccttataa atggttccgt ctaaatcaat taaatagcct ttatatttca tctatttctc
     1381 cctaaacctt ttttatttct tgccaagtaa tgattgcttg ggcattgata accccatcac
     1441 ttgtaatttc atgcttgctt tccagtccag tccgttcaac agccgatgta atcaccccac
     1501 ctggtcgaac ttccttgaca tacttgaggt tgattttctt gggaatatag tgggtcaaaa
     1561 aatccgctcc catgacctca aaaatccagt ccaagtattt actgttattg acatgaccat
     1621 tcatatccaa gtcgtaaaaa cgaacatggt aatccttgct gatcggttct tccaaggact
     1681 catacttcgg tccacggata agttttttat caaaatcaga ctggtaagga gccacaatct
     1741 caggttcaac agcatggact tttcgactgt cgcggtccat gagaacaaag gtcgccatca
     1801 tatggatgag ctcctgcccc gcttcattat aaatagtaaa gcgacggtag caaaaaagtc
     1861 gattgtagct caaggcttcc gtttcgatgg taatttcttc cgcaaaacga ggcaaacgaa
     1921 ccacctcaat atcatattct gcgataatcc agaccagatt atagtcttcc aaaatggcct
     1981 tatcactaac tcccagttca atcgattgca tccctgaaac ttgcagtgac agcaaaatca
     2041 catctggaag tttgatatga ccgttcatat cagccatatc aaaaggaatt ttcattttca
     2101 tttgataagt taagcccatg atcctactcc aaaataaatc gttctgctac agtatctccc
     2161 aaaaagagac ctctctttgt catgcgaacg tggtcaccct caatctgcat gaggccttgt
     2221 tgaaccaaat ctctgacaat ttctccataa agtccagcaa aagactgtcc aaatttttcc
     2281 tcaaatcgcg ccatggaaac cccggatttc ttgcggagtc ccaagaacat ttcttcttct
     2341 atttgctcct tttgactcag gtgatcttct gtaatacaag cattgccttc ctcaaccgca
     2401 ctgagataat gacgaatggg accatgattt ttatagcgta ctccattgac ataaccagat
     2461 gcccctgcac caataccata gtattcagca ttgtcccagt acatgagatt gtgacgactt
     2521 tcaaaaccgg gtttggagaa attagaaatc tcataatgct caaaacccgc tcgctccagc
     2581 tctgcaatga tgtactcaaa catctccgct tctagttcct ccttaggcag aggcaatttc
     2641 ccacgtcgca tccggttcat aaagaccgta tggttttcta aaatcaaact atacaaactc
     2701 atgtggggaa tatccaatcc aatggcttta gccatatttt cctttacttg ctccatggtc
     2761 tgaccaggca gagcataaat caaatcaatg gagatgttgt caaaaccagc cagtttcagg
     2821 cgatcgatat tttcataaat atccttctcc aaatgactgc gcccaatctt tttcaacatc
     2881 ttatcatcaa aggtctggac acctagcgaa acacgattga cagccgaatt tttcaaaaca
     2941 gctatcttat ccgcatccaa atcgcctgga ttggcttcaa tggtcaactc ttccaagaca
     3001 gacaaatcca agtttttagt caagccattc agtaacacct ccagttgcgg agccgacagg
     3061 gctgtcggtg ttccaccacc gatataaagg gttgacaact tttcaatatc ataagaacga
     3121 aactcttcca gcagatgctc taaatagctg tcgactggct gatttttgat gaagaccttt
     3181 gaaaaatcac aataataaca aatctgggta caaaatggga tgtgcacata ggctgacgtt
     3241 ggttttttct gcatagtaat tattatacca caaagactag attccagata aaaatcacca
     3301 tccccagata catagtccgt ccggagatgg tgatggttta ttcttctgtt atatcaatca
     3361 caatctcttc tgagtcatca agagcttcgg ctttttcttg ccattgttcc ttgagattat
     3421 ttaattgatt ttttgatgct tctgtcgctt gaaaagcata ggatttagct tgagcaagta
     3481 tactgtccac agtgatttca cctgactcaa cctgttcttt tgttttcaga acaaaatctg
     3541 tagcctgctc cttaacttct gtcagttttt cacagacttg ctccttggca tactccggat
     3601 cttctctcaa atcatctaga aaatcttgag cctgactgca aacttgtttg cccttatcac
     3661 ttgttaaaaa caaggcaaga gctgcacctg aaacggttcc taaaaggatt gaggataatt
     3721 tacccataag gattctcctt ttttattttt tgaaaaattt acttgcaaga cgaagagctg
     3781 acagacttgc accagtcttg agtgtttttg aaccagctga tgaagctttc ttgctcaaga
     3841 cacgcgcatg gtcattgagg tctgaaacag atagagataa atctgcaaca gcactgaaga
     3901 gtggatcaat cgtagccacc ttgacattga tatcatctgc caagacattg accttagcca
     3961 acaactcatt ggtgtgatgc aaggtcacat ccacatctga agtcaaggtt ttaatcgtct
     4021 tttctgtttc atcgatgaca cgaccaagct tttgtacagt aatgatcaga tagaccaaaa
     4081 agacaatcaa agctagggca acaagaatat atgcaacttc taacatttag ttttcctcct
     4141 ctgtaatata gtaaggggcc ttctttcgat tttgataaat aacgatcatt ataccgagac
     4201 cgataaagac aactgacagc cattgggaca ctcgaaggcc gaagaacatg agactatctg
     4261 ttcgcatacc ttcgatgacc atacgaccga aaccatacca aatcaagtaa aaggccgtga
     4321 tatgacctcg tctgagactc ttccatttcc gtctaaaaat cagaatcaag gcaaagccaa
     4381 gcagattcca tagagactca taaaggaaag tcggttgacg gtagctcccc tcaatataca
     4441 tctggtcacg gataaagcca ggtagataat ccagattatc cactgttgca ccataagctt
     4501 cttggttaaa gaaattaccc caacgcccca aactttgagc aatcataacg ctaggcgccg
     4561 caatatctag aaaatcccaa gtattgatga gtttacggtc agcaaagata tagagcacaa
     4621 gagccccagt tatcaaacca ccgtaaatgg ccaaaccacc attccaaatg gcaaaaatct
     4681 ctcctaaatt ctgactatag taatcaaatc ggaaaataac atagtagaga cgagctccta
     4741 aaatagccaa gggaaaggct actaagataa aatctaaaat atcgtctggt atgatcttct
     4801 ttctaggtgc ttctttcatg gtcaaataaa ccgcaagaat caagcctgtc acaatacata
     4861 aggcatacca acgaatggct aggggtccta gttgaatagc aattggatca agcattttgc
     4921 acctcatttc gagcgattag acttgtcagt cgttcgtcga acaaacgggt cgcatcaaag
     4981 cccatttcct tggcacgata attcatggca gctgcctcaa tcacaacaga gatattacga
     5041 cctgttttaa ctggaatacg aatacgagga atggctacgc cagaaacttc aagttcctct
     5101 gcattgtttc caagacgatc aaaggtctta tgcgtatcgt aattttccaa atagacagca
     5161 agctgaacct gtgaagaatc cttgacagca ctcgcaccgt agagactcat aacatcgata
     5221 ataccaaccc cacgaatttc aatcaagtgt ttcaaaattt cagctggttc accccagaga
     5281 gtaatctcat ccttggcaaa gatatcgaca cggtcatcgg ctaccaaacg gtgaccacgt
     5341 ttgacaagct caagacctgt ctcgctctta ccaattccac tatctccctg aatcaagacg
     5401 cccatcccat aaatatccat caagacaccg tgcacactgg tacgttctgc caaacgagaa
     5461 tccagatagc tagataactc tccagataaa cgactggtag ctgcacggct ggttaaaata
     5521 gcaatcttac attctctagc agcctttaac atctcctctg gaaccaccaa accacgggca
     5581 acaatgactg ctggtgtctc aggtagaaac atttttttca aaacttcata acggctgttg
     5641 gaaggcatgc tgatcagata agaccactcc ttcatcccca aaagttgaat ccgttctggt
     5701 gtatagtagt caaaatagcc tgtcatttca agaccaggtc gcgtaatatc cgctgtattg
     5761 atttcctttt caagcaattc tggttcgcca tagacaatgt ctagtctaag cttttcaatc
     5821 acttctttta ctaaaaccga catcatttcc tccttcaaat gagttcttct ctctattata
     5881 tcaaattgta ggaggaagtt tcagattttt gcaggctttg gagtatttat ttaaaagaaa
     5941 aaggactaga tttctctagc ctttcatttc aattagaatt ttttacgttt acgagctact
     6001 tctgatttac gtttacgttt tacagaaggt ttttcataga attcacgttt gcgtgtttct
     6061 tgaagagtac cagctttagt aaccgcacgt ttgaaacggc gaagtgcgtc gtcaagagat
     6121 tcattcttac gtactactgt tttagacatt tttttcactc ccttcaagtt caatatctat
     6181 ttcattctac acgtaaaagg aataattgtc aagcaaaaat gcggtttaat tttgattttt
     6241 tcttccattt atacaagagt tgaaagcgtt caatagaaca ttgctttttt atttttcgag
     6301 taagctaagc gcttcagcat ctgcgatgat ggttacatca gggtgatttt ggaggctact
     6361 cgcaggtagg ttctcagtca ctgggccaga tactgttccg gcaatggctt ctgctttcga
     6421 ctcaccataa gcaaaaagaa taatagactt ggcatccaaa atgtttttaa tccccattga
     6481 aatagcttgg gttgggacgt cttcaatctt ggcaaagaag cgagcattgg cttcgatagt
     6541 agactggtca agttctacta gatgcgtttg actgtcaaat ggagtaccag gctcattaaa
     6601 gccgatatgc ccattgcgac cgattcccaa gatttggagg tcaactggat ggtcagccaa
     6661 aatttggttg tagcgttcaa cttcagcttc agcattgtcc ttaacaccac gaggcaagaa
     6721 actttcttta aatggttttt ggttgaacaa gttttcttgc atgaagtaac gataagactg
     6781 tggattgtcc ccatcaaggc ccacatactc atcaaggttg acactggtta gatttgaaaa
     6841 atcaaggtca ctctcaacaa tttccttgta aaattcaagt gggctacttc ctgtcgcaag
     6901 tcctagagtt tgagctccat tagccaattt ttccttcaaa atctcaaaag caacttttcc
     6961 accttgaact tggttttcaa ctttaataac tttcatcttt tcatcctcca tttctgtcta
     7021 tatttattat atggtataga ccaatttttg tcaagtgaaa acgatttaat ttctaaaaaa
     7081 agagcgtcca aacttgaaac gtgttataat ggataggatt agaacttaga aagaatgaac
     7141 agatatatga atacagctga ttttgatttc cacttgccag aggaattgat tgcccaaacg
     7201 ccccttgaaa aacgtgatgc ctccaaactc ctcatcgtca accgtgagac aggagaaatg
     7261 caagataaac acttccactc tattattgat atgctggaac ctggtgatgc ccttgtcatg
     7321 aacgacaccc gagttctccc tgcccgcctc tatggtcaaa aagtggagac aggaggtcat
     7381 gtggaacttc tcctccttaa gaacactagt ggagacgagt gggaagttct ggctaaacct
     7441 gccaaacgcc tcaaggtcgg tactcgtatc agctttggtg atggccgcct cagcgctgtc
     7501 gttacagaag aattgaccca cgggggacgc attgtccgct ttgaatacca aggaattttc
     7561 ctagaagtct tggaaagtct gggagaaatg cctctgccac cttatatcca cgaaaaatta
     7621 gatgaccgtg aacgttatca aaccgtctac gccaaggaaa gcggctctgc tgcagcaccg
     7681 actgctggtc ttcacttcac caaagaactg ctggcagaaa tccaagctaa gggtgttcat
     7741 ctagtctatc tgactctcca tgtcggactc ggaaccttta gacctgtttc tgtggataat
     7801 ctggacgaac acgaaatgca ctcagagttc tatcaacttt ctgaggaagc tgctgccacc
     7861 cttcgctctg tcaaaaaaaa tggtggtcgt gtcatcgctg tcggaaccac ttctatccgc
     7921 accttggaaa ctattggttc caagtttgat gggcaaatcc aagcagattc tggttggacc
     7981 aatatcttta tcaaacctgg gtatgagtgg aaggtcgtgg atgccttctc aaccaacttc
     8041 catctgccaa aatcaactct ggtcatgttg gtttctgcct ttgcaggccg tgaattagtc
     8101 ttagatgcct accaccatgc catccaagaa cactaccgct tcttcagttt tggtgacgcc
     8161 atgtttattt attgagaaag aatttctcta attcttctaa taccaataaa tcgctaagat
     8221 attatttcaa agaacatcta caattgaaac tctagctagc tgtagaagac gcctagtaca
     8281 ttgaaattaa aatgcttccc cctagcttcg aaaatattgc catagattgc gttgactctc
     8341 caaattgatt catctatatt ttatttcagc ttcctatatt ttcttcgctg tttgtaaatc
     8401 aaaatgcaag acacatgagt agcaccatat ttgttactct tatctgtcct ctcaagagac
     8461 tattatgagt tatttcagaa tcattcacta ctttgaccct gactctcctt agtctcaaaa
     8521 tcaaagactt atactcttca aaaatctctt caaaccgcgt caacgtcacc ttggattata
     8581 tatgtgactg acttcgtcag ttctatctac aacctcaaag cagtactttg agcaacctgc
     8641 gactagtttt ctagtttgct ctttgatttt cattgagtat taaacaaaaa gtgaacaaat
     8701 ctgaattcta atgtacagaa gactaggctt gttcactttt ttatagtcgc tataagatga
     8761 ccttatctat ggctttttat atataattat atattcagac atactattat caattttgtc
     8821 gcagggagga atctgttaac gcacccattc accattatca ttgactctat agccatctat
     8881 acttgtattg accgctaact cacccgatgc atttacataa taccatttac caccaacttg
     8941 gaaccattga ttgactttca tagaaccgtt gctgttgagg tagtaccatg aactattaac
     9001 ttgtacccaa cctgttgcca tggaaccatc agtattataa aaataccaca taccattttc
     9061 ttgtttccag tctgttgttg gagcaactgc tttagctggt tctactgcta catctgttcc
     9121 ttggttagat gtaacagata caggatacga aggaatagat gattgctcag gaacaacaac
     9181 tttttcaggt tctctcgtcc ctctccttat acgtcttttt accatctctt tagtaatttg
     9241 acgagaagta gtttcttcaa ttgttccatc acgttcatct acagtataga ttgtagtaag
     9301 agtaatttac caatttctcc tacttcttct acttcttgac ttttatcaag agttgggcca
     9361 tcgagatatt ctgtttcgat tggaatttct tggacaagaa cttggggctt ggttcttttt
     9421 ttaacaactc ttgtttgaga gtcttttttt tgacttaaag tactctcagt tacttgtcca
     9481 ctctttccat ctacattata agttatcgtt gtaactgttt tcccattctt tcctagagta
     9541 atctcttgct cctgtcctgc agaaaggtca ttgtctgctt catatttagt agcaaatgga
     9601 acaagaactt cttcaacctt gcttttagct ggaactttga taactgtatc cgtggcttct
     9661 tttctatcaa cagtaacctg ttcggtaaca taaccagtct ctggattaac atcgtaggtc
     9721 cttgtcgtag ttacatagcc atcctctcca tcaattgtaa caggattttc actacggtct
     9781 tttgtttcat ctttttcata acgaattcgc gtacttgaaa ttttcttggt tactacctta
     9841 ggtttagtcg ctacttttac aataatatcc ccattgtcag cgtcatcata ctctattccc
     9901 tcttctttat ctctagtatc atctctgaca tattgaatcc catcagcagc atgaacaaaa
     9961 cttgtattca gattcctcct aaaaataaag ttagcccgat taccgcagaa ccaaaaatct
    10021 ttccgagttt acgtattgca tagcgcttat tagtattaga ttttgccatt acatcctact
    10081 tctagtatag catcttttct atc
//
LOCUS       DAVHEX010000070         9728 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVHEX010000070 DAVHEX010000000
VERSION     DAVHEX010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9728
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.071"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            229..324
                     /locus_tag="VVL45_001866"
                     /pseudo
     CDS             229..324
                     /locus_tag="VVL45_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(577..1197)
                     /locus_tag="VVL45_001867"
     CDS             complement(577..1197)
                     /locus_tag="VVL45_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU4187999.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLYTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTPNTWNDTSYQS"
     gene            complement(1197..1679)
                     /locus_tag="VVL45_001868"
     CDS             complement(1197..1679)
                     /locus_tag="VVL45_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEU4188000.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDQMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(1679..2521)
                     /locus_tag="VVL45_001869"
     CDS             complement(1679..2521)
                     /locus_tag="VVL45_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEU4188001.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDFKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDFLGTFFPIGIGAGATLIVFSKLIDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(2616..3416)
                     /locus_tag="VVL45_001870"
     CDS             complement(2616..3416)
                     /locus_tag="VVL45_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEU4188002.1"
                     /translation="MRKQIHPLILFSFFGIMMFILIINRPLNDSQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            3560..4939
                     /gene="glmU"
                     /locus_tag="VVL45_001871"
     CDS             3560..4939
                     /gene="glmU"
                     /locus_tag="VVL45_001871"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEU4188003.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIASEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVIVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGNNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            4949..5494
                     /locus_tag="VVL45_001872"
     CDS             4949..5494
                     /locus_tag="VVL45_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU4188004.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            5506..5820
                     /locus_tag="VVL45_001873"
     CDS             5506..5820
                     /locus_tag="VVL45_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HEU4188005.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            5837..6529
                     /locus_tag="VVL45_001874"
     CDS             5837..6529
                     /locus_tag="VVL45_001874"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEU4188006.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNAQEQVVFGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQKSLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            6606..7097
                     /gene="rocS"
                     /locus_tag="VVL45_001875"
     CDS             6606..7097
                     /gene="rocS"
                     /locus_tag="VVL45_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HEU4188007.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            7195..7785
                     /locus_tag="VVL45_001876"
     CDS             7195..7785
                     /locus_tag="VVL45_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEU4188008.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLDLSQLFD"
     gene            7794..9557
                     /locus_tag="VVL45_001877"
     CDS             7794..9557
                     /locus_tag="VVL45_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HEU4188009.1"
                     /translation="MKPEKPKKLGLKQIYLDLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDRYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
BASE COUNT         3007 a         1684 c         2073 g         2964 t
ORIGIN      
        1 agttcatgta cacctgatat gatgcgtttt ataattttaa agactttttg accagcctca
       61 tttttttaac ttgatactca gtgaaaagca aagattaaac taggaagcta gctgtaggct
      121 gctcaaagaa cagctttgag gttgtagata aaacttgtga ggtcaccaac atatataatg
      181 tgaagctgac gtggtttgaa tagattttag aagagtatga gtctggaagt tttaatggat
      241 aatgcaagat tccatagaat gggtaagcta gagttcttat gtgaagagtt tgggcataaa
      301 cttttacctt ttcctcccta ctcatcttag tatagaaaag tgaatctgaa atagtacata
      361 actgcttcta aaacattctt ataaattgat ttaaattctc aaatcatatt attcagttct
      421 tatttcattt tgttctacaa tcctgttgag aagacacgtg ttcatatcaa aaaggtattg
      481 gcaagttgca ataccttttt acgaggctct gttgtcttat ttttgtttca actgactata
      541 tctcctatgg ttctagttca gaaggctagg ctataattat gattgataag aagtatcatt
      601 ccaagtattg ggagtgaatg tttcaaaatc atgggtttct ataatggtca ggctggcatt
      661 tgctagaccg ccatctttac gaagaagtgg ttctttatag cctaggagag tacgaagact
      721 ggcagtaaga ttggcgccgt gtccgacaat tagaatacgt tcagctggac tatcttttaa
      781 tgatttgata aattggatgg tccgttgagt tgtactatag agggattcgg ctccgaacat
      841 tcgagtgtca aattgagcaa gatttgaacg aaaagcctgg atttgttgcg ggtaaatagc
      901 ttccaaggtt gcaattttca aaccttctaa cttcccaagt tgccattcac ggagattagg
      961 aacgatttct aaagaacagg gggtatagag ttgactttgg ataatctcag cagatttgac
     1021 cgctcgaggt aaatcacttg aataaatctg atcaaaagga atttccttga gatactgacc
     1081 aagtcgtttt agggtttcaa tggattcagg aagaagagga gaatcaccac tagcaccttg
     1141 aaaacgacct tcttggttcc agagggtacg accgtggcgg acaaagtaga gtttcattac
     1201 ttgatgtcct ccaaaatatc tacaaagtct gcctttacaa agctagccaa gtcttgtggc
     1261 gcgacgataa tgctgtgtcc gacttcgcct gcagagacaa tcatttgatc caaatctaga
     1321 gcaattttat cgataaaaat gggataattg tgtttctgac gaattccgac aggattattg
     1381 gctccatgaa tgtaaccagt tgttttttct aagtcctttt gtggaatcat gctcactttt
     1441 ttattgccag aaattttagc tagtttcttt tcagacaagt gctgagtgat agggacaatt
     1501 ccgataatcg gtccggtctt gtctcccaaa agcgccaagg ttttgaaaat ctgatctcgt
     1561 tcataacctt gaggaagctc tccttctagg gcattgattt gaatcccctg atgagggata
     1621 gctgctttag ataggatttg ttccaccaat gtttttttga ttttaacttt ttttgccatt
     1681 atttatattt atcctccaat tgactcatcc aaataccaag ccagattccc agcgcaaaga
     1741 agaaggcgat gatgacataa ccgacaagtg aaagtcctgt gtattggata ctttcagcgt
     1801 ttcctgcatt tggaattaag atcaaaaggg tacttgatag gacgataccg atgatgaaat
     1861 gatagacgcg tgagtggtag ttgtttaagg cataatctat caattttgaa aaaacgatga
     1921 gagttgcacc tgcaccaatt ccaatcggaa agaaggttcc caagaaatca aaagttttaa
     1981 aaccagtcaa cataggagca tagagtccca aaatcaaaag taaatttgat gggctgaggc
     2041 caggaaccaa gacgccaagg gccaatagtg cgcctgctag gacgaagtta agaaagctgg
     2101 cgcttaaggt tccaacgaca aaatttaagg catagagtcc taatccagaa atgataaagg
     2161 ttgtccataa ccaagctaaa tcaatcttgt ctcggtcaga ttctcgagtt gattctttga
     2221 ggaggctagg aactgtaccg ataatagctc ccgcaaagct ccataataca aaaacctgat
     2281 aattttcaag caggtattca atcgggtagg aaaataagcc gattcccaga agcataccga
     2341 tggcaactgg aataaagtac aaaacatttt ctttaaagtc tttaaaggga tgggccagaa
     2401 agccaatcat tcgttcatag attcctaaga ttgctgctag aacccctccg gaaattcccg
     2461 gtaggataaa tccaagagca attacaatcc ctttaataac gcgtgctaac catgagagca
     2521 tatatttcct ccaactattt ctatttcaaa aaatccttac tatattgtat cacaatcaga
     2581 cacaaaaaga aaaagcaaat gataaacaaa tgcttttaag gttttaaaaa aagttttcga
     2641 aaggtttctt ctttattttt taagggagag ataacgttga tatctaaatc gtggtcaaag
     2701 ccggcaattt ttcctttaga tgtgtattgg tgaatatcat aatctaaatc agttttagga
     2761 ctgctctcca aaaatcctga gtctgagccg taggacggaa tccaaacaga ggtaaacttg
     2821 cctgtatcaa tactgtgttc ttccatgaag tagacaccaa cgtagatgcc gatgttttta
     2881 gcacccagtg atgctagttt tgctcgaaag ttttcgacac cttcgttcat attagacatg
     2941 gttttgtctt ccacgtcaag ccaatagtaa ctagggctgt aaggagaggc agcattgtag
     3001 aaaacttcgg cagccttttc catttcttgg acactttttc cagctacata agcgtagaca
     3061 gcaactggga cattccgctt ttgaagttca gtgatatgac tcttataggc cttgtctatt
     3121 ccattgataa atgaagcatc attttctttt gtcgtttgag caccactgtg aacacgaaca
     3181 atagcacctg aaatattttg tgagagggca tcgtagttga tttcctcagg acgctgccag
     3241 ccagagaggt caataatcgg tttgtctaag tgtttcaaag cctgtgcttc aatctgtgct
     3301 atattggatt ttgttttaaa cgattggctg tcattaagtg ggcgattgat gattaaaatg
     3361 aacatcataa tcccaaaaaa actaaataaa ataagtggat gaatttgttt tctcatatct
     3421 tataattcta ccctaaaaat caaaaaaaat caaaaaaatg ggttaaggaa gagactttag
     3481 agcatttttt cattcaagag tgcggaatga tttgaaatat ggtataataa aagggaattt
     3541 ctacagaaaa gagaagatta tgtcaaattt tgccattatt ttagcagcgg gtaaagggac
     3601 tcgcatgaaa tctgatttgc caaaagtttt gcacaaggtt gcgggtattt ctatgttgga
     3661 acatgttttc cgtagtgtgg gagctatcca acctgaaaag acagtaacag ttgtaggaca
     3721 caaggcagaa ttggttgagg aggtcttggc tggacagaca gaatttgtga ctcaatctga
     3781 acagttggga actggtcatg cagttatgat gacagagcct atcttagaag gtttgtcagg
     3841 acacaccttg gtcattgcag gagatactcc tttaatcact ggtgaaagct tgaaaaactt
     3901 gattgatttc catatcaatc ataaaaatgt ggccactatc ttgactgctg aaacggataa
     3961 tccttttggt tatggacgaa ttgttcgtaa tgacaatgct gaggttcttc gtattgtcga
     4021 gcagaaggat gctacagatt ttgaaaagca aatcaaggaa atcaacactg gaacatacgt
     4081 ctttgacaac gagcgtttgt ttgaggcttt gaaaaatatc aataccaata acgctcaagg
     4141 cgaatactat attacagacg tcattggtat tttccgtgaa actggtgaaa aagttggcgc
     4201 ttatactttg aaagattttg atgaaagtct tggggtaaat gaccgtgtgg cgcttgcgac
     4261 agctgagtca gttatgcgtc gtcgcatcaa tcataaacac atggtcaacg gtgttagctt
     4321 tgtcaatcca gaagcaactt atatcgatat tgatgttgag attgcttcgg aagttcaaat
     4381 cgaagccaat gttaccttga aagggcaaac gaaaattggt gctgagactg ttttgacaaa
     4441 cggtacttat gtagtggaca gcactatcgg agcaggagcg gtcattacca attctatgat
     4501 tgaggaaagt agtgttgcag acggtgtgat agtcggtcct tatgctcaca ttcgtccaaa
     4561 ttcaagtctg ggtgcccaag ttcatattgg taactttgtt gaggtgaaag gatcttcaat
     4621 cggtgagaat accaaggctg gtcatttgac ttatatcgga aactgtgaag tgggaagcaa
     4681 cgttaatttc ggtgctggaa ctattacagt caactatgac ggcaaaaaca aatacaagac
     4741 agtcattgga aacaatgtct ttgttggttc aaattcaacc attattgcac cagtagaact
     4801 tggtgacaat tccctcgttg gtgctggttc aactattact aaagacgtgc cagcagatgc
     4861 tattgctatt ggtcgcggtc gtcagatcaa taaagacgaa tatgcaacac gtcttcctca
     4921 tcatcctaag aaccagtagg agcctatcat ggagtttgaa gaaaaaacgc ttagccgaaa
     4981 agaaatctat caaggaccaa tatttaaact ggtccaagat caggttgaat taccagaagg
     5041 caagggaact gcccaacggg atttgatttt ccacaatggg gctgtctgtg ttttagcagt
     5101 aacggatgaa caaaaactta tcttggtcaa gcagtaccgc aaagctatcg aggctgtctc
     5161 ttacgaaatt ccagccggaa aattggaagt aggagaaaac acagcccctg tggcagctgc
     5221 ccttcgtgaa ttagaggaag aaacagccta tacagggaaa ttagaactct tgtacgattt
     5281 ttattcagct attggctttt gtaatgagaa gttaaaacta tatttagcaa gcgatttgac
     5341 aaaagtggaa aatccgcgtc cgcaggatga ggatgaaacc ttggaagtcc ttgaagtgag
     5401 cttagaagaa gcgaaagaat taatccaatc aggtcatatc tgtgatgcca agacaattat
     5461 ggctgttcag tattgggagt tgcagaaaaa atagaggagg tcagtatggg taaatcttta
     5521 ttaacggatg aaatgattga aagagctaat agaggcgaaa aaatttcagg tcctcctttg
     5581 ctagatgata atgaggaaac taagatttta ccaacctctt cttcccgttt tggttatgcc
     5641 aatcctaagg atcatggttt tagccaggaa accttgaaga ttcaggtcga accatctatt
     5701 cataaaagcc gtcgtattga aaataccaag agaaatgtct tcaattctaa gttgaataaa
     5761 atcttatttg cggtcatctt tctcttgatt ttgcttgttt tagcaatgaa acttttgtaa
     5821 tagaaaagga attgaaatga aaataggaat tattgctgct atgccagaag aactggctta
     5881 tctggtccag catttagata atgcccagga gcaagttgtt tttgggaata cctatcatac
     5941 aggaaccatt gcttctcatg aagtcgttct tgtagaaagt ggaattggta aggtcatgtc
     6001 tgctatgagt gtggcgattt tggctgatca tttccaggtg gatgccctta ttaatacggg
     6061 ttcagctggg gcagtagcag aaggtatcgc tgttggggat gtcgtgattg ctgacaaatt
     6121 agcctatcat gacgtggatg tcacagcttt tggctatgct tatggacaaa tggcgcaaca
     6181 accgctttat ttcgaatcag acaaaacctt tgttgctcaa atccaaaaga gtttatctca
     6241 attggaccaa aactggcatc ttggtttgat tgctacagga gatagttttg ttgcaggaaa
     6301 tgacaagata gaagcgatta agtcccattt cccagaagtt ttagccgtgg agatggaggg
     6361 ggcagctatt gctcaagcag cgcatgccct caatctccca gtcttagtca tccgagctat
     6421 gagtgacaat gccaaccatg aagcaaacat cttttttgat gagtttatta tcgaagctgg
     6481 acgtcgctct gcccaagtct tgttgacctt tttgaaggct ttagattaag cggaaatttg
     6541 acagtttttc tagcttatga taagatttaa gtaaagaaaa gctagaaaac gtttcagagg
     6601 atattatgag tattgaaatg accgtcagtg agattgcaga ggtcttagga ttatctcgcc
     6661 aagcaatcaa taaccgtgtc aaagaattac cagaagaaga cacagataaa aatgacaaag
     6721 gggtaacagt tgttaccaga agtggcttga ttaagctaga agaaatctat aaaaaaacga
     6781 tttttgaaga tgagcctgtc agtgaagatg tcaaacaacg tgaactgatg gagattctag
     6841 tggatgagaa gaatgcagaa attcttcgtc tttatgaaca attaaaagcc aaggatcgtc
     6901 agttatcaga aaaagacgag cagatgcgta tcaaagaccg tcagattgct gagaaagaca
     6961 aacaattaga tcagcaacaa caattgaccc ttcaagccat gaaggatcaa gaaaatctta
     7021 agctagagct ggaccaagca aaagaagaag tccaatccac taaaaaaggc ttttttgctc
     7081 gtttatttgg aggataatca aggagctgtt taggttaaat gctaacctga tggcttcttt
     7141 tgtttgtgaa tagtatagtt gattagaaca gagacagtag aggagaggta cagaatggaa
     7201 cgattagaag attacattgc ttttgattta gaatttaatc agcacgaagg acaaacacac
     7261 ttgattcaag tatcggcagt gcgttttaga gatggtcggg aaatcgatgc ctatgattcc
     7321 tatgttcata ccagcgtccc tctaaaaagc tttatcaatg gtttgaccgg gattacagct
     7381 gagaccttaa aagatgctcc tccagttgag aaagtcttag aagaattcca agagtttgtg
     7441 ggttctttgc tgatgatagg ctacaatgct gctaaaagtg atttacctat tttagcagag
     7501 catggtttag actatagcca acagtacaag gtggatttgt atgatgaagc ttttgaacgt
     7561 cggagttcag atttacatgg tattgccaat ctcaaattgc aaactgttgc ctcatttctt
     7621 ggatttcaag gtcagtctca taatagttta gaggatgctc ggatgacggc gcgtgtttac
     7681 gaatccttct tagagtcgga tcaagctagg gaattgttag agacagaaag tagcttatca
     7741 aacaatcctt ttggtggctt ggacttgtct caattatttg actaggagtg cctatgaaac
     7801 ctgaaaaacc taaaaaattg ggtcttaaac agatttacct cgatcttgat aaaatcctct
     7861 ttctattttt cttgattttt atgatagtgg agtatttgtg ggtaccattc aattcatggt
     7921 tggctagctt cttattagat cagacaggtt atctttttat ttcctataac aatgtcttag
     7981 ccatccttac tcattctcca ttgattagtc tagctttttt agccttgatt gtaattaacc
     8041 ttctggttgc ttattttcaa ataggactcc tctttattgg tgctcgtcat ctcctctata
     8101 atgaaaagcg aaggctaatt gagtatatcc gcaaggtttt ccatgaaagt tttggattta
     8161 tgaaaaagct aacttgcgct aaagccttgt ttatcttttt ctatatagct atgctgtttc
     8221 cttttatacg gaaaatgctg aagatttatt atcttaataa aatcgtcatt ccagagttta
     8281 tccaagctta tttagaagat agatattgga tgtggtggct gagcatccta cttctatctc
     8341 ttatctttct ctatgtctct gtcagattga tgtttgccct tccgaagatt gtatatgatc
     8401 agctgactgt tcgagaagca gtgatgttta gcttggagaa aaccaagaag agagttactt
     8461 tttatgcttg gaatttattt tacatcttgc tcaaagcaca tttattattt tatcttcctt
     8521 tgattccttt attattggct caaactttgg tagatgatat cactcagaga gagtctctga
     8581 ttcttggtat tttgaatttt gtcgtgatta aaaatctcta ttatatggct ctgacttatt
     8641 ttttggttaa atttgtttca tttttgacag gaaaggagct ggatatgctt cctaggagag
     8701 aaaaagatca catcgtgcga tggggtgtca tgacttgtgc cagtcttttc tttgccttag
     8761 aaggttatat ttatctggag gctcccatgg ttcatctacc tcaacttatt tctcaccgag
     8821 gggtttccaa tgcaaatggg attcaaaata cagtagagtc cttggaaact acagcacaac
     8881 tcaaaccaga cttggtggag acggacgtgc aggaaacaaa agatgggcag tttgtcatga
     8941 tgcatgatgc taacttgaaa aatctagcag gtatcaataa aagtcctcaa gacttaaact
     9001 tggaggagct taaagggatt gatatttttg aaaatggcta ccagactaaa atttcaagct
     9061 ttgaagatta tctcagtcga gccaacgaac ttggtcaaaa attactaatt gaaattaaaa
     9121 ccagtaaaaa agatagtcca gacatgatga accgcttttt agcccgttat gctgcaaagc
     9181 tcaagattta tggacatcaa atccagtctt tagactacca tgttgtcgaa aaagtaagac
     9241 agtatgatgc cgaactacca gtttatttca tcatgcccta caattctgtc tttcctaaaa
     9301 caagagcgac aggatatact atggagtact caaccttgga tgaatatttt gtaagcaaac
     9361 tatggacaac ggatcagaaa ctttatgtct ggactgtcaa tgattcagaa gctatcagca
     9421 aatctcttca cttaggagta gatggagtga ttacagatga tttggaaaaa gttcagcggg
     9481 aaatagaagt agcccaagaa gatccagaat atacggattt gctcttgaaa aaagcattgg
     9541 aattctttga attttagtaa taaaaaagag gaacggttgt tcctcccatg ataggtaagt
     9601 attcttgtga atgcttgctt ttttgtggtt gcaatccgtt tacgagaatt gtttgtattg
     9661 ttcttgtttc aatttacttt atagtgcttt gaagttggaa tgctacacta taacttataa
     9721 aatatagt
//
LOCUS       DAVHEX010000071         9649 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVHEX010000071 DAVHEX010000000
VERSION     DAVHEX010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9649
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.072"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            345..932
                     /locus_tag="VVL45_001878"
     CDS             345..932
                     /locus_tag="VVL45_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188010.1"
                     /translation="MFVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNILSDEVLE
                     VVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVNTIHGNKQ
                     ANTFANFMTGAGISYMIGRIGALLGGPWGAIIGGAYFGIQAYQSYLDSQSPYPYYITS
                     TYIHVAQRKWKFITEYYRNSNYTGYVKTVTTYVNF"
     gene            complement(1019..1222)
                     /locus_tag="VVL45_001879"
     CDS             complement(1019..1222)
                     /locus_tag="VVL45_001879"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188011.1"
                     /translation="MPKWSNPDYVNELDPKIVDMLVEFHKSQGTLETPEAQAEIAQKR
                     EEIEQRRAELEGKKQELLNRLNK"
     gene            complement(1519..2223)
                     /locus_tag="VVL45_001880"
     CDS             complement(1519..2223)
                     /locus_tag="VVL45_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEU4188012.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAYLNPAVTIGVALKGGLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            complement(2293..4119)
                     /gene="glpO"
                     /locus_tag="VVL45_001881"
     CDS             complement(2293..4119)
                     /gene="glpO"
                     /locus_tag="VVL45_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEU4188013.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKDQVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKHLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELTLSPVDFLLRRTNHMLFMRDSLD
                     SIVEPILDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            complement(4161..5669)
                     /gene="glpK"
                     /locus_tag="VVL45_001882"
     CDS             complement(4161..5669)
                     /gene="glpK"
                     /locus_tag="VVL45_001882"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEU4188014.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQMDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            complement(5828..7255)
                     /locus_tag="VVL45_001883"
     CDS             complement(5828..7255)
                     /locus_tag="VVL45_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU4188015.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELAIHSEDENLRLSIGAATKGRDIRSLFLESAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSAGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLVAIRQLLFT"
     gene            7420..8292
                     /gene="hslO"
                     /locus_tag="VVL45_001884"
     CDS             7420..8292
                     /gene="hslO"
                     /locus_tag="VVL45_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEU4188016.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGTGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            8279..9259
                     /gene="dusB"
                     /locus_tag="VVL45_001885"
     CDS             8279..9259
                     /gene="dusB"
                     /locus_tag="VVL45_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEU4188017.1"
                     /translation="MTNLNTPFMIGNIEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIETLLQLEKA"
     gene            complement(9489..>9649)
                     /locus_tag="VVL45_001886"
     CDS             complement(9489..>9649)
                     /locus_tag="VVL45_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choline-binding protein A"
                     /protein_id="HEU4188018.1"
                     /translation="DTWYYLEASGAMKASQWFKVSDKWYYVNGSGALAVNTTVDGYGV
                     NANGEWVN"
BASE COUNT         2755 a         2197 c         1924 g         2773 t
ORIGIN      
        1 ttattgtttt ttattgtaga gcagacccaa gcgaatgaac ataggttgta aaaagcaaat
       61 gaaaaaactg cgaggataat agcttctgtg tctaaataac ccatttcgag acctcttttc
      121 aacaacaaca agcctgtagt ttcgataacg atcaaacata aaaatatata aaaaattttc
      181 gcattaagca tattgttacc gctttctttt tatttggttt tattgtataa taaaagttga
      241 aaaaaatcaa aaatacaaag gagtttccgt tatgtctaag atgtttaaat gcatgtgttt
      301 gctagttatg tctgttttct gtatgtctag catttccccg tcgcttgttt gtttttgctg
      361 atgatagctt atcggcaaac tctaaagttg tatctggtga ggctcagttt gagaatggct
      421 catcagtgcg gttcggagat acgcaagtaa atattttaag cgatgaagtt cttgaggttg
      481 tcaatccaga tggaagtgta gacactattg aacgacgtgc tgacggcgtc tatatcaatg
      541 gggcatttta tatggcttat caaaagaatg aaatcgacct aaatatatct tttagatcat
      601 acgatcctaa tgtttggaat tacgtcaaca caatacatgg gaataaacaa gctaacacat
      661 tcgctaactt catgactggt gctggaatca gttatatgat tggtaggata ggtgctttat
      721 tggggggtcc atggggtgca ataattggtg gagcatattt tggtatacaa gcttatcagt
      781 cttacttgga ttcacaaagc ccatatccat actacataac aagtacttat atccatgttg
      841 ctcaaagaaa atggaagttt atcactgaat actatagaaa ctcaaactat actgggtatg
      901 taaaaaccgt aacaacctac gtgaatttct aaaatataaa ccctgcctag atataaaggc
      961 agggtttata ttttagttaa ttgctcaagt aatttgtaag cataatactt gcgaaactct
     1021 atttgttcaa gcggttcaaa agctcttgtt ttttaccctc aagctcagct ctcctttgct
     1081 cgatttcttc acgtttttgg gcgatttctg cttgcgcctc gggagtttcc aaagtgcctt
     1141 gtgacttgtg aaattctact agcatatcaa cgatttttgg gtctaattcg ttgacataat
     1201 caggatttga ccatttcggc acgttgcttg caggtgcttg ctttttgccg ccttttttgg
     1261 gcgttttggc ttgttgttca gctttctgac tgtagttgac ctcgtggaat tttcgtgctt
     1321 gatatgatgc gttttattat gagaagattt acttcatttt ttgctgaaat tgggctcttg
     1381 cccaagctct atcaacataa ttggtttcta atactcaatg aaaatcaaag agcaaactag
     1441 gaagctagcc gcaagctgta cttgagtacg gtaaggcgac gctgacgtgg tttgaatttg
     1501 attttcgaag agtataaact agaaaagtga gaatacaagc actgccaagg ctgctccgat
     1561 aacagggcct acaacaggaa tccaagcgta agaccagtct ccgtctccct tgtttggaat
     1621 tggcaagatg ctgtgcatga tacgaggtcc aaggtcacga gctgggttca aggcataacc
     1681 tgttgtccca cctagtgata gaccgatacc gacaatcaaa gttcccactg caaaggttcc
     1741 gatacctgcc tgaaagtcgt aaagacccaa agcaaagatt gtcaacacca aaacaaaagt
     1801 tccaaggatt tcgctaatca agtttgatac agtatccttg atggctggtc cagtactgaa
     1861 ggttgccagg atattgcctg cattttcttc tgcctcatag tgaggtttga attgcaacca
     1921 aaccaaaatc tgacccagca tggcccctgc gaactgggct aagatataag gcaaaacgga
     1981 agcccaaggc aaaccacctt ttaaggccac accgatggtc acagctgggt ttaaataagc
     2041 tggactgagc ttgccagata caaagactgc aaccgcaact gcaatccccc aacccatagt
     2101 aatcacaatc caacctgagc tattgctctt ggttttagga agaaccacac ctgcaacaac
     2161 accatttcct agaagaatca ggattaaagt ccctagaaat tctccaaata attcattcat
     2221 catctttctg tctccattaa aaagaaggga cgggcgacaa ggaatgctgc cctccacctc
     2281 ttttattttt tcttaatttt ttaattctgc taaatcgttg ttagcgagag ctgcttcgac
     2341 atcagcacgg taagttgctt tttcttcttc tgtccagtca tagaatcgtc ccatttcatc
     2401 caaaattggc tcaacgatac tatccaagct atcacgcata aagagcatgt gattggtacg
     2461 acgaagaagg aagtcaactg ggctaagagt caactcattg cgcattgcat agtgaaggga
     2521 caaagtatct gccaagctga gtcctggcgc ttgttccaag ctgtgagcaa gtgcaaagac
     2581 tttcggtgca tttgaaccgt aaagatttgc cagatagtga gcttccttgc tatccaaacc
     2641 acgtgatact ccaagttgcg caaaggcttc gatttctgaa tccacatttg ctgggttcaa
     2701 ttctccacct gaaacagggt aagttttaga attgatcaat ttaaagctac ggtcaaattc
     2761 tgctttgagg atgtcaacca cgcgctccat agctccttca gccatcttac ggtagtctgt
     2821 gattttacca ccagcaagag tcaagagacc attgtcatca cggtccaagc tagacccacg
     2881 agaaactgca gatggatcca aatgtttctc agatgtgcta ctttcaagct tgctgacagc
     2941 agactcaaca tcttcacgtg ttttttcttt ggagagataa gattcaacag tcgcaatcaa
     3001 gttgtcaaag ctttcatcac tgatggtacc gttatttcca ccattatagt cagaggcact
     3061 gttccctgca atcaatggac gaagacctgc ccagctgctt tcgatatcat caatggtgat
     3121 gttggattct gggaagcggt tgttgacaat gccaagtaga taatctacat cttcttgagt
     3181 tacttttgga tgctccaaat cacctgtgta gtctgtatca gttgtaccaa agtaagtctt
     3241 gttttcacgt gggagaacaa agaccatacg accgtcaccc aaacctgtgt caaagtaaac
     3301 tggctgtgaa accttgattt tgcttgaatc cactaccaag tgaactccct tagttgggcg
     3361 catttgtgag aattgcgttc ccttattaga caaattacgt actttatcac tccaaggacc
     3421 tgttgtatta ataaccagac gggccttgat ttcaaatact tggtctgtca agagatcacg
     3481 agctacaaca cctgtaatct tgccactttc gtcaaagagg aagccttctg ccttcacgtg
     3541 gttggcaatg agggcaccgt cttggttggc acgtttgatg ttttcaatca cgagacgcgc
     3601 atcgttgtta cggaagtcaa gatacactcc acctcctacc aagccttcct tcttcaagtt
     3661 tggctggcgt tccaagactt gatccttgct caaaaccttg ttcgcagttg gtgtgttgct
     3721 cacacctgct aaaaggtcgt acaagtccat ggctacttta agacggaaga ggctaaaggt
     3781 tgctccatct tcatcgtaaa ctggtaagag cattggatct ggttttggaa tgtgtggagc
     3841 gatttgttga accactgcac gttcagaaac cgtatctgag accacctcta cgtcaaattg
     3901 tttgaggtaa cgaagtcctc cgtgaaccaa ttttgttgaa cgactagatg ttccttctgc
     3961 aaagtcttgc atttcaatca aaccagtctc aagaccgcta gctgccgcct gcaaggctac
     4021 accagctcct gtgattcctc caccgataat caagaggtcc agggtgcgtt cctgcatttt
     4081 tttaattgac aattcacgtg ttttctttga aaattccata tttacacact ttctaaataa
     4141 atcgctttat tctgccagta ttagtcgtct acttccgcaa agacttgagt tgctttcaca
     4201 gccttcttcc agcccttgta gagttgttcc ttgcgagatt cattcataga tggctcaaag
     4261 agttctcctg tctcgttcaa gagtttcaac tcgtccaagt ctttccagta ccctactgac
     4321 aaacctgcta ggaaggccgc tcctagagct gttgtttcca agtttttagc acgtgcaatg
     4381 tcaatgccta aaatatccgc ctggaactgc atgaggaagt tgttcatggc tgcaccacca
     4441 tccaccttca gtacttgaat ggcggtctga gtatccattt gcatggtgtc gatgatgtca
     4501 cgcacttgat aagcaataga ttgcaaagtc gccttgataa agtcttcttt gcttgttcca
     4561 cgagtcaaac caaagacgga accacgagca ttttggttcc agtatggagc gcctagacct
     4621 gtaaaggctg gaacgacata aacttcatcg ttgttgtgag aatcacgagc gtatttttca
     4681 gattctggtg aattttcaac catgcgaaga ccgtcacgaa gccactgaat agcacttcct
     4741 gcgatgaaga tagaaccttc caaggcataa taaaccttac cgttgattcc gtaaccaatg
     4801 gttgtcaaga ggttgttttc agacaactgc atctcttccc cagtattcat gatgatgaaa
     4861 gagcctgttc cataagtatt cttaaccata cctggctcaa aagccaactg tccaaagagg
     4921 gctgcttgtt ggtccccagc catacctgag attggcacct ctccaccgta gaaatggaat
     4981 ggagctgtct tgccgtagat ttcggagtta gaacgaactt ctggaagtat agccttcgga
     5041 atgttaagga tttccaaaat ctcatcatcc catttgagtt ctttaatgtt ataaagcatg
     5101 gtacgagctg catttgagta gtcagtcacg tgagccgcac cgtcagtcaa tttccaaacc
     5161 aaccaagtat cgatagtacc aaagagcaat tccccttttt ctgctcgctc ttgagcacct
     5221 tctacatgat ccaaaatcca acgaaccttg gtagcagaga agtaagcatc aataatcaaa
     5281 ccagtctttt catggaattt ttccacataa ccttggcttt ttagttgctc agccaaaggt
     5341 gctgtctggc gtgactgcca aacgatagca ttgtagatag gaagtcctgt tttcttatcc
     5401 cagacaaccg ttgtttcacg ttggttggta atcccgattg cctcgatttg atttggcttg
     5461 acaccacttt cgatgaaagc acccgcaata actgactgaa cagagttcca aatttcattg
     5521 gcattgtgct caacccaacc tgcctgaggg aaaatctggg taaactcttt ttgactcgag
     5581 ctaacctttt cccctttttt gttgaaaatg atggcacgag aacttgtagt tccctggtca
     5641 atggccatga tgtatttttc ttgtgacatg tactgtcctc ctgataaaga tcttgaggtg
     5701 tttcctcttt acacttacta gtatacaaaa taaagcgctt tcttgtttat caactttttt
     5761 taatttttaa aaaaatgtga ggagattgtg tgaatttcac aaaaaagacc tggattaacc
     5821 aagcctttta agtgaataat aactggcgaa tcgctaccaa atcctccata tccaagtcat
     5881 tttttaaata atagactggt gtctgttcct tcttatgaat cgggttattg gtaaccagca
     5941 aatcataatg ccgactaggg tcataagctt caatggtaat aaaacgattg tattccaaat
     6001 accgtttcaa aatggctgcc atccttgaaa agacaagaaa accagatgtc aaatccagac
     6061 caatccgcat atgctggcca ccgttttcag ccatatattc gattaactgg agccattccc
     6121 agagaatctt cttatctaaa tctgtcccct gttgaaaagc aggtagagca tgaaaaatct
     6181 cctctgctgt ctctttaaaa tcatgttcca tcagtaaata tggatgacga ttctctaact
     6241 ggtacttgta gcgatcctgt aaaatatagc ccttatagag atagacttga gcacaaagct
     6301 gactgagttc ataggtgaca tggtcctctg tataatcccc caatagttcc tccttcttca
     6361 tttcttgaat caattgcgtc agtaaatctg ccaactgccc tccaaaacca agaatatact
     6421 ccatagtatg aagaggaaga atgcgatgag ataggagaaa agagaagaat atcatcatct
     6481 caccatcctc aactcctagt ggaatatgtt gcccagcaaa aaaggacgga acctttctca
     6541 gcaaacgaag ataaaagata ttgtcaaaat accctcgcat tttctcttct atgacttgaa
     6601 actgacaagc attgacccga agacgttgtt gactgatgtg agcccagaga accaagtcca
     6661 atttctgccc cgcagacaaa cttgcaccgc agatttcctc taaattggca atctcctgtt
     6721 ttctctctgg tttctgcatg tgaccttccc attcctgact cgaccagacc tttcggaaaa
     6781 gacagaaata gaaatagcga atctgatgct ctggacctcg ccaacggcca ttttggatgg
     6841 ataaatcaaa ttctgacaaa atctgattta aaccagccaa gtgacgacca agcgtagcct
     6901 cgctaatcac caattcttga gccagctgat gggctaaaaa ctgttggtgg tagagaagat
     6961 aaaccaaaat ctggtattta acagcactct ccaaaaacaa gctccgaata tctctcccct
     7021 tggtagctgc accgatagac agacgcagat tttcatcttc tgagtgaata gccagctcta
     7081 agccactatc caaagccttg tcattgagca gggtgacata tttggttagg gttgcttttg
     7141 aaaatcctgt ttcttccatt acagccttga cggtcgtctg agactcttgt aatagaaagg
     7201 aaaggattga aaattgacca cactcggctt tctccatcaa atctcctaaa tacatttgtt
     7261 atccctttca ttttctacct taagcatagc ataaatctga caaatgctaa aataatctgt
     7321 ttctcttttt attttcatgc tgatttcctg gtccattatc ctgaaaatca gtaaacacac
     7381 ggctccccct ttgggcattt ttatgctaaa atagtagcta tggataaaat tattaaaact
     7441 atatcagaaa gcggagcctt tcgtgctttt gtccttgata gcactgaaac cgtccgcact
     7501 gctcaagaaa aacatcaaac ccaagctagc tcaactgtag cgcttggtcg aactcttatc
     7561 gctagccaga ttctcgcagc caatgaaaaa ggaaatacca aacttacagt taaggtgttg
     7621 ggatctagct ctctaggtgc tattatcacc gtcgctgata ccaaggggaa cgtcaaaggc
     7681 tatgttcaaa atcctggtgt tgacatcaaa aagactgcga ctggtgaagt cctagtcgga
     7741 ccttttgttg gaaatggtca attcctcgtt atcacagact acggtactgg aaatccttac
     7801 aactctataa ctcccctcat ctctggagaa atcggtgaag accttgcctt ttaccttact
     7861 gaaagccaac aaacgccttc agcggtcggc ctcaatgtcc ttttggacga ggaagacaag
     7921 gtcaaggttg caggtggttt cctagttcaa gtcttgccag gagccaagaa agaagagatt
     7981 gctcgctttg aaaaacgcat ccaagaaatg ccagctatct ctactcttct cgaaagcgac
     8041 gaccatatcg aagccctcct caaggctatc tacggggacg aagcctacaa gcgtctttct
     8101 gaagaagaaa tccgtttcca atgtgactgt agccatgaac gctttatgaa cgctcttgcc
     8161 agccttccaa gctcagactt acaggaaatg aaagaggaag accacggggc agaaatcact
     8221 tgtcaattct gccaaactac ttacaacttt gatgaaaagg acctggagga actcattcgt
     8281 gacaaatctt aatacacctt ttatgattgg caatattgag attcccaatc gtaccgtttt
     8341 agcgcctatg gctggcgtga ccaactcagc ctttcgtact atcgcaaagg agctcggagc
     8401 tggactcgtt gtaatggaaa tggtctctga caagggaatc caatacaaca acgaaaaaac
     8461 cctgcacatg cttcatatcg atgagggcga aaaccctgtc tctatccaac tttttggtag
     8521 cgatgaagac agcctagcac gcgcagcaga attcatccaa gaaaacacca agaccgatat
     8581 cgtcgatatc aacatgggct gccctgtcaa caaaatcgtg aagaacgaag ctggtgctat
     8641 gtggctcaag gatccagaca agatttactc catcatcaac aaggtccagt ctgtccttga
     8701 tatcccactt actgtcaaaa tgcgtaccgg ctgggcggac ccatctcttg cagtagaaaa
     8761 tgctctcgct gctgaagctg caggtgtttc tgccctcgcc atgcatggcc gtacccgtga
     8821 acaaatgtat actggccacg cagaccttga gaccctttac aaggttgccc aagctctaac
     8881 caagattcca ttcatcgcca acggtgatat ccgtactgtc caagaagcca agcaacgcat
     8941 cgaagaagtt ggtgctgacg cagtcatgat tggccgagct gccatgggaa atccttacct
     9001 cttcaaccaa atcaaccatt actttgaaac aggagaaatc ctacctgatt tgacctttga
     9061 agacaagatg aagatcgcct acgaacactt gaaacgattg attaacctca aaggagaaaa
     9121 cgtcgcagtt cgtgaattcc gcggtctcgc tcctcactat ctccgtggaa catctggcgc
     9181 tgccaaactc cgtggagcca tttcgcaagc cagcaccctg gcagagattg aaaccctctt
     9241 gcaattggag aaggcttaat agtttaaaac ccgtaactct cttaaagagc ctcttgaatg
     9301 ccgccattct ttcctcatca aaaaaactcc gtagtctcta gcgctataaa tccggctaga
     9361 gattacagag ttatttttta atctaaactt cctacaaggg tatattatct actgaaagga
     9421 tatggtattt gtagggaata ctttaaagag ttcttacagg aagtcagtat taactagtta
     9481 tattaggttt agtttaccca ttcaccattg gcattgactc catagccatc tacagttgtg
     9541 ttgactgcaa gggcacctga gccattgaca tagtaccatt tatctgatac tttgaaccat
     9601 tggcttgctt tcatagcacc tgatgcttca agatagtacc aggtatctc
//
LOCUS       DAVHEX010000072         9616 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVHEX010000072 DAVHEX010000000
VERSION     DAVHEX010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9616
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.073"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..836
                     /locus_tag="VVL45_001887"
     CDS             <1..836
                     /locus_tag="VVL45_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU4188019.1"
                     /translation="HFKHDFSCLPEIMSWDEYAFTKGKMSFIAQDFNNLNIITVLKGR
                     TQAVIRNHFLKYDRAVRCRVKIITMDMFSPYYDLARQLFPCAKIVLDRFHIVQHLSRA
                     MSRVRVQIMNQFHRKSHEYKAIKRYWKLIQQDSRKLSDKRFYRPTFRMHLTNKEILNK
                     LLSYSQNLKHHYQLYQLLLFHFQNKEPEKFFGLIEDNLKQVHPIFQTVFKTFLKDKKK
                     IVNTLQLPYSNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERTKFVLSR
                     A"
     gene            complement(887..1822)
                     /gene="dapA"
                     /locus_tag="VVL45_001888"
     CDS             complement(887..1822)
                     /gene="dapA"
                     /locus_tag="VVL45_001888"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEU4188020.1"
                     /translation="MSYQDLKKCKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKTIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(1878..2954)
                     /locus_tag="VVL45_001889"
     CDS             complement(1878..2954)
                     /locus_tag="VVL45_001889"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEU4188021.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGSSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPRDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIAAF
                     PGAVLEDDVAHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(3296..3368)
                     /locus_tag="VVL45_001890"
     tRNA            complement(3296..3368)
                     /locus_tag="VVL45_001890"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3333..3335),aa:Thr,seq:ggt)
     gene            complement(3468..3977)
                     /locus_tag="VVL45_001891"
     CDS             complement(3468..3977)
                     /locus_tag="VVL45_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEU4188022.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(3975..4071)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            4100..4531
                     /locus_tag="VVL45_001892"
     CDS             4100..4531
                     /locus_tag="VVL45_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEU4188023.1"
                     /translation="MKKPIQKFFNNEILAYLFFGIVTTLVAILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKVYIFRK"
     gene            4613..4990
                     /gene="mscL"
                     /locus_tag="VVL45_001893"
     CDS             4613..4990
                     /gene="mscL"
                     /locus_tag="VVL45_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEU4188024.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKEE
                     KTDEKKPTELEVLQEIKALLEKK"
     gene            complement(5032..6126)
                     /gene="hemH"
                     /locus_tag="VVL45_001894"
     CDS             complement(5032..6126)
                     /gene="hemH"
                     /locus_tag="VVL45_001894"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEU4188025.1"
                     /translation="MKKAILMMTFGSPEEIIFEGVADFFTNIRRGVRPQDHEIQTLYD
                     NYVRIGGTPLQKITRQEVALVEARLGSEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEALLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESQDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            6281..7504
                     /gene="pepT"
                     /locus_tag="VVL45_001895"
     CDS             6281..7504
                     /gene="pepT"
                     /locus_tag="VVL45_001895"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEU4188026.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTTLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEA
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSDRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(7540..8169)
                     /locus_tag="VVL45_001896"
     CDS             complement(7540..8169)
                     /locus_tag="VVL45_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188027.1"
                     /translation="MSKANQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDPQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSAKSKDKTALLESTKAILVLNHQIQSKASASEETSPARNAEANGDNTSAENQP
                     NATAESNIETASDENKPSNTRDSKPAESTSENETTESSTTTGNQEKPVE"
     gene            8134..8355
                     /locus_tag="VVL45_001897"
     CDS             8134..8355
                     /locus_tag="VVL45_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188028.1"
                     /translation="MDLFQELICLAQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            complement(8626..9616)
                     /locus_tag="VVL45_001898"
                     /pseudo
     CDS             complement(8626..9616)
                     /locus_tag="VVL45_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149760.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
BASE COUNT         2891 a         1958 c         1730 g         3037 t
ORIGIN      
        1 ttcactttaa acatgatttt tcttgtcttc ctgagattat gtcttgggat gagtatgctt
       61 ttacaaaagg aaagatgagc ttcattgcgc aagattttaa caatctcaat atcatcactg
      121 ttcttaaggg aagaacacaa gctgtcatcc gaaatcactt tcttaaatat gatagagccg
      181 tccgatgtcg cgtcaaaatc attactatgg atatgtttag tccttactat gacttagcta
      241 gacaactttt cccgtgtgct aaaatcgtgt tggatcgctt tcacattgta caacatctta
      301 gccgtgctat gagtcgtgtg cgtgtccaaa tcatgaatca gtttcatcga aaatcccatg
      361 aatacaaggc tatcaagcgc tactggaaac tcattcaaca ggatagccgt aaactgagtg
      421 ataagcgatt ttatcgtcct acttttcgta tgcacttaac caataaagag attttaaaca
      481 agcttttgag ctattcacaa aacttgaaac atcactatca gctctatcaa ctcttgctgt
      541 ttcactttca gaataaggaa ccggagaaat ttttcggact cattgaggac aatctgaagc
      601 aggttcatcc tatttttcag actgtcttta aaacctttct aaaggacaaa aagaaaatcg
      661 tcaacaccct tcaactaccc tattcaaacg ccaaattgga agcgaccaat aatctcatca
      721 aacttatcaa gcgcaatgcc tttggttttc ggaactttga aaacttcaaa aaacggattt
      781 ttatcgctct gaatatcaaa aaagaaagga caaaatttgt cctttctcga gcttagctga
      841 cttcaaccca ctacagttga caaagagccg gattttattg tctttattag taatctggtc
      901 ttaagacccc tgttacagtt gccttagttg cttcgtagtc gccatctacg acaaccttga
      961 taatgcgttt ggcatcttct tctggtgctg gaacaagagg tagacgagtg ggtccagctt
     1021 caaatcccat atagttaaga attgccttaa ctggagcagg acttggataa gagaagagag
     1081 cattaacctt aggaatgaat ttacgctgaa ttgctgcggc tttcttcata tcgctttctg
     1141 caatcgcagt aaacatctcg tgcatttcat ccccatttgt atgagaggca acagaaataa
     1201 ccccatccgc cccaaggttc atggcatgga aagcatctcc atcctcacct gtataaatca
     1261 agaactcttc aggcttgtgc tcaatcaagt aagccatatt agccaagcta gtacattctt
     1321 tgacaccgat aatatttgga tggtcagcca agcgaagcat ggtttctgga gtcaattcga
     1381 caactacacg ccctggaatg ttatagataa taattggtag gtcagaagca tctgcaatag
     1441 tcttaaagtg ctgatacatc ccttcttgag aaggtttgtt gtagtaagga acaatagcaa
     1501 gcccagctgc gaaaccacca aattccgcta cttctttgac aaactcaata gagtcacgcg
     1561 tatcattagt acctacaccc gcaatcaaag gaacgcgtcc attgacaacc ttttgtacag
     1621 ccgcaaacaa ctccaactcc tcatcgtggg tcaaagttgg actctcagca gtcgttcctg
     1681 cgagaagaat tccatccgta tgatgggcca ataaatgctc aatcaaggct ggaatagcat
     1741 caaagttaat ggaaccatcc tcatggaagg gggtaataaa ggctgtaatg attttacatt
     1801 tttttaaatc ttgataagac acgaaaacac ctctctattt caaagaagga gttcatctga
     1861 actcctaaac gatatgacta ttttaattca aatttcaatt cggctgttgg acgaaccaat
     1921 ccacgttcat gaagagtttc agcaatctga actgagttcc aagcagcacc tttgagaagg
     1981 ttatctgaaa caacccacat gtgaattcct ttttctgcat ccaagtcttt acggatacga
     2041 ccaacaaagg tatcacgcga accaactgca ttgatagctt gaggatagat ttgatgagct
     2101 acatcatctt caagaacagc acctgggaag gctgcgatag ctgcttttac ttcttcgatt
     2161 ggagccactt cttttgtttc gatataaaca gactcagagt gagctgacaa gactggaata
     2221 cgcacacatg ttgcagatac tgcaatgcta tcatcttcca taattttctt agtttccttg
     2281 gtcatcttca tctcttcgta cgtgtaatca ttatcagtga aaacatcaat ttgtggaaga
     2341 gcgttaaagg cgataggata atgtttcttg tcaccacctg aaggcaagat ttccgcatgc
     2401 aaatcacgtg gtttcacacc atcattcaag acttcacgaa gttcacgttg tgtctcaaga
     2461 attgctccca taccagcacc tgaaacggct tgataagttg aaacaatgat acggtccaag
     2521 ccccattttt ggcgaaccgg ctcaagagcc accatcattt gaattgttga acaattaggg
     2581 caggcaatga ttccgttgtg agcatcaagt gcatgagcat tgacctctgg aacaaccaaa
     2641 ggaacatctg gattttgacg gaaataagat gtattatcta ctactaccac gccagctttt
     2701 actgcgtatg gtgcatactt agctgatgta gaactacctg ctgaaaagag agcaatatca
     2761 actccttcaa aagctgtttc agtcgtttct tcaattgtaa tatcttgatc tttaaatttc
     2821 aatgacttac ctgctgaacg tgcagaagca aggtaacgaa ttttatcgat tggaagtgtt
     2881 gattcttcca acatttttat catctgagca ccgacagcac ctgtcgcgcc gactacagca
     2941 actgtatatc ccataaataa cctctttcgg aattttctaa aaatttctat aatagaagta
     3001 ttatactact ttttccatga attgcaaagc tttttcatca ttttttggaa aataaaaatc
     3061 ccagtcgcta gactagggac taagaggcat cttcatgtga tgagcaggtt cacacaactc
     3121 atcaaggtcc gctcctgcgt tatgacctcc tcatgctcaa tagtagtccg aagactacct
     3181 atcgactcat cgcatattct atattactag agatacttga ttttgtcaat aagaaatttt
     3241 tcagatcgag tataacaaaa aactctcgta ttcaagagtt tccctataca tatcatgcta
     3301 attgccggga ttgaaccggc gacctcatcc ttaccatgga tgcgctctgc caactgagct
     3361 aaatcagctt acttaagaag tatactatat tcagaaaaac ttgtcaagtt tccttagaaa
     3421 ttttccatag gaaaaagcca ggtaaaaact acctagctta tcttcttcta tttacttaaa
     3481 tcaattcgac gaccaatttt accaataata atcgctccaa taatcaatga agcaaattca
     3541 ccgattcctg ttgtaaacca agtaaggaag aatggtaatt ctgctacaat atttaactct
     3601 gcagcgatag taaacatgga aattgaaaag aggattgaaa agaagaaatg atcttttcga
     3661 atcaatccgt taaagaggta atccttgcta tattttgcaa aaagccaaac acctaaactg
     3721 aggaatacta aggttgaacc accaccaaca aagacatcta gcagtccgaa gctaaagaaa
     3781 ttagcaatca tacaaccgat ggtaactccg atgatgtact taggattgta aaaagccata
     3841 aagttcatca tttctgaaat acgaaactga taagcaccat agctgatggc atttaggggc
     3901 ggtgtgacag tcaaaacgac gtagatagca gcgacgattg caatatctgc aacatcacga
     3961 atagttaatt ttttcatctt ttctcctttg gcggttatcc gcgtgtaata tgcttggtga
     4021 aagaagctaa gcaccaaggg ttgattcatt caaccttact agtatagcac aatagcctgc
     4081 tttatgctat acttaaagca tgaaaaaacc tattcaaaaa ttttttaaca atgaaatctt
     4141 agcctatctc ttttttggta tagtgactac tcttgttgct atactatcac gattagtcat
     4201 ttatcagcta agtcatcagg aactccttgc tacggccttg gcaaatatta tcggtatcct
     4261 atttgccttt atcacaaatg atacgattgt atttaagcaa aaaaagaaaa atagacttat
     4321 ccgtttagtg aaattttctc ttgcccgcct ttctactttt ctgttagact tgtttttaac
     4381 ttttctcttt gtaacccaat ttcctcacat catagggcaa ttcgtaactg aaaacattga
     4441 taagataaat gctattgaaa caataattgc acaaatattc attattatca tcaattatat
     4501 cttgagcaag gtctatattt ttagaaaata atgctccatt tatgagcctt ttaattgcct
     4561 tctataatga tttaggatac aatgaaacac gaaacattga aaggaaaaca gtatgttaaa
     4621 aaatctaaaa tcgttcttgc ttcgaggaaa tgttattgac cttgctgtcg gtgttgtaat
     4681 tgcctctgct tttggtgcta tcgttacttc acttgtaaac gacattatca ctcctcttat
     4741 tttaaatccc gctttgaaag ctgctaaagt tgaacgtatc gctcaacttt cttggcatgg
     4801 agtcggctat ggtaacttct taagtgctat tatcaatttt atctttgtgg gtaccgccct
     4861 cttctttatt atcaagggca ttgaaaaagc acagaagctg actggcataa aggaagaaaa
     4921 aactgacgaa aaaaaaccaa ccgaattgga agtccttcaa gaaataaaag ctctccttga
     4981 gaaaaaataa tcgattaaaa ggatctagcg caaagctaaa tccttttttc tttactcttt
     5041 gggtaacttg cctgcttttt caagcatttt tttgataaga taaggcatct taacattttc
     5101 acgacctttt ttctcaatca attttttcac aaattctggc atttgtaaat cttgagattc
     5161 agccaaaatg tttttcgtct cgtctgaagg aactaactca tcaaaggtct cttcttctgg
     5221 gagaaattcc ttaaactctt gattttcatt gactctaact gtattgacca aagcatcaat
     5281 caaacgagaa tctgtatttg gcattggtgg acgatggtag tttaccccaa attcctgaca
     5341 taagtcataa cattccacat cattgtcaaa caagacctca atatgctcac tgatgaaact
     5401 aataggtaca aaaatataat ggtctggatg ttctgtctgt tctctgagat actccaaaac
     5461 atctggttta atccatggaa taccaatatc actctcactt tgccaagtgt tggtatattg
     5521 ttctgaactc aagcccagtt tttcagcgac tagcttgcta ttttcgaaaa tttggtctat
     5581 ataaggatca ccaaagtcca aggcaaaaat aggcacacta tgggctgaaa agatgacttt
     5641 aaagctgtct tgctttactt cttcttttaa aatcttagca atttcatctg cccaatagtt
     5701 taagagcgct tcttcctgat accagtcctt aatgaccaaa aattgaattt gtttgctttc
     5761 taaaaatttt tcatagccca tgacagagta gaaagaataa tggggctcca aaatcaagca
     5821 aatacactgt tcaattccgt ctgcttccat ttgaccgatc acatctggaa taaagggact
     5881 ggaaaactta ttagcaaagt agacactgta ttcactcccc agcctagctt ctaccaaggc
     5941 aacctcttga cgggtaattt tttgcagagg cgtgcctcca attcgtacat aattatcata
     6001 aagagtctga atctcgtggt cttggggtct caccccacga cgaatatttg tgaaaaaatc
     6061 agctacacct tcaaagataa tctcttctgg tgaaccgaag gtcatcatta aaattgcttt
     6121 tttcatgact gcttccttgt gatgttttta tcaagtttat tttatcatta attcatcgat
     6181 aagtaaacgc tagcataggg acattctcaa tcttctgtct tttgtttttc tcttctttct
     6241 atgatacaat ggaaaaaata aattcaaaag gagttttttt atgacttatc caaatctctt
     6301 ggaccgcttc ttaacctatg ttaaggtcaa cacgcgctct gatgaacact ctactactac
     6361 tccaagtaca cagagtcagg ttgacttcgc aacaaatgtc ctaattcctg aaatgaaacg
     6421 tgttggactg caaaatgttt actatctacc gaatggtttt gctattggaa ccttgccagc
     6481 caacgatccg tctttaacac gtaagattgg ttttatatcg cacatggata ctgctgattt
     6541 taatgctgaa ggagtcaatc cacaggtaat tgaaaactac gatggtggtg tgattgaact
     6601 agggaattct ggtttcaaac tcgatccagc tgacttcaag agtcttgaaa aatatccagg
     6661 acaaacgctc atcacaacag atggaacaac cttgctaggt gctgatgaca agtcaggaat
     6721 tgctgaaatt atgacagcca ttgaatatct aactgctcat cctgaaatta agcactgtga
     6781 gattcgtgtt ggttttggtc cagatgaaga aatcggtgtt ggtgccaata aatttgatgc
     6841 agaagatttt gatgtggatt ttgcctacac tgttgatggt ggtccactag gtgaacttca
     6901 gtacgagact ttctcagccg ctggtgctga attgcatttc caaggtcgta atgtccaccc
     6961 tggtactgcc aaagggcaga tggtcaatgc ccttcagcta gcaattgatt ttcataatca
     7021 acttccagaa aatgaccgac ctgagttaac tgaaggttac caaggttttt accatctaat
     7081 ggatgtgaca ggtagtgttg aggaggcgcg tgcaagctac atcattcgtg attttgaaaa
     7141 agatgccttt gaagcgcgta aagcatccat gcaatctatc gctgataaga tgaatgaaga
     7201 acttgggagc gaccgtgtca ctctcaactt gacagaccag tactacaata tgaaagaagt
     7261 cattgaaaaa gatatgactc caattaccat tgctaaagcc gttatggaag atctaggtat
     7321 cacgcctatt atcgaaccaa tccggggtgg aacagacggc tctaagattt cctttatggg
     7381 aatcccaact ccgaatatct ttgcaggtgg cgaaaatatg cacggacgtt ttgaatacgt
     7441 tagccttcag actatggaac gtgcagttga taccatcatt ggcatcgtag cttataaagg
     7501 ctaaaaagac gaggtagctc agctacttcg cctttctttt tattctactg gtttttcttg
     7561 atttccagta gttgtagaag attctgttgt ttcattttct gaagttgatt cagcaggttt
     7621 agaatctctt gtattgcttg gtttgttttc gtcgctagca gtttcaatgt tagattctgc
     7681 agttgcgttt ggttggttct cagcactggt gttatcacca tttgcttcag catttcttgc
     7741 tggacttgtt tcttcacttg cgctagcttt tgactggatt tgatgattca aaactagaat
     7801 agcttttgtc gattcaagta aagctgtttt gtctttactc ttagcagaaa gttgatctaa
     7861 taatgcatcc accttatcaa agtccgcatc agatccatta ttactttcta aataagagtg
     7921 aagcgacatg agaatatcgt agagtttttg atagagtaca agtgtctgag gatcttgctc
     7981 agcattttcc ttttcttgtt gaagggcgct agcgatacga gtcaagacat cttttacctg
     8041 actgtttact tcatccaagt ctgcatcagc cttgtttgtg gcagctttta gattttctac
     8101 ttcttctgcc aaggattgtc tgattccttc ttcatggatt tgttccaaga gttgatttgc
     8161 cttgctcaaa agactttcta cttcttcctt gctatctgtc gcagattatt ggttgctatc
     8221 taccatgtac tcctaaaaca ggagagttat aatccaagat tacaaggcct tacagaaata
     8281 agaaatccag ataagacaat gttcgtccaa gacgctattc gcttcgcaca gcagcacgga
     8341 ttcaatatgc tttaatttta aagtttaggt gtcaagacct ctttttagtg tgcccaaaat
     8401 ttagagaagt aatcaatcaa ctaactttta tttttttcaa actttcagta aactgaccta
     8461 aagctaactc aatctgtctt tgtagatgct tctgctatca gctagaagtt gatctacttt
     8521 tgccaagact gccttctcat caaaagttcc aggttgatag ttggattgca gggatggaat
     8581 cttgtttttc aaagccgctt catatccctt agtttgaacc ttgatgtagt gattgtggtc
     8641 gccatgagga atcacaaaac cttctgaatc ttcacttata attcgattgg catcaaaacc
     8701 atgaccatct tcttcctcat gatggacatg tagtgacgga ttacttaata cagaactaga
     8761 agaacttcct acctcttccg tgttagagtg tgatggggga ttgttaagag atgacttagg
     8821 aatatagtga tagtgatccc catgtcttac tatataagca tcacctgtat ctctgacaat
     8881 atcattaggg ttaaagacat atgtggctgc taattcacct gccgacaagt cactctcagg
     8941 aatgaaatga tagtgaccac catgtggtac tatagtagat tgaaatagaa tatgagcaaa
     9001 ttgataaggg gattttaaag taatttctaa caatgattta gaaactatga tgtgctattc
     9061 taaattcaac tcactatata taaccatcat cggtagtata acgtccctgt aattttgcta
     9121 cagatacttc tgcactagct cctttatcgt ctttaccatg ttcttgtttt tggcgattga
     9181 tttcatcttt tgttcgtaca ttttctgcat gagcttgatc tttaaggtaa acataatact
     9241 ttccatctac cttaataata tatcctccct taacctaact gacgatatct tgatctttcg
     9301 gctgatagtt gggggctttc attaatagct cttcactaaa gagcgcatca aaaggaactt
     9361 taccattata gtagtgataa tgatcgccat gagaagttac ataaccttga tctgtaatct
     9421 taataacaat ttgttttgct tgaattcctt ctttttgact aacctagtct ggagtcaaat
     9481 tttcagtctt cttagtgtct ttattactgt ttacatatga aacacgattt ttatctgtat
     9541 tggcctgtta gctatgttgg ttcagagcat aaacacacag acttaaggaa aggataacaa
     9601 cagatccagc tgctat
//
LOCUS       DAVHEX010000073         9358 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVHEX010000073 DAVHEX010000000
VERSION     DAVHEX010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9358
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.074"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            328..1614
                     /locus_tag="VVL45_001899"
     CDS             328..1614
                     /locus_tag="VVL45_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEU4188029.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAVTEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESPEKFEAIKQDLVDEMWKVAQRELATGFYYGTPSENEQLFGARRKIPEYKFVAEVV
                     SYDDAAQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELL
                     TIKVPQPVQSGDMVRALKEGLINLYKEDGTSVTVRA"
     gene            1629..1940
                     /locus_tag="VVL45_001900"
     CDS             1629..1940
                     /locus_tag="VVL45_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188030.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LEHHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCPFCFSRFNPGCQNHKERYF
                     K"
     gene            2186..2914
                     /locus_tag="VVL45_001901"
                     /pseudo
     CDS             2186..2914
                     /locus_tag="VVL45_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
     gene            3249..3983
                     /locus_tag="VVL45_001902"
     CDS             3249..3983
                     /locus_tag="VVL45_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4188031.1"
                     /translation="MRIKEKTNNINGGIKNVSKHYGHSIILKDINFALNKGEIVGLAG
                     RNGVGKSTLMKILVQNNQPTSGNIISSDNVGYLIEEPKLFLSKTGLENLKYLSNLYGV
                     DYNQERFRCLIQELDLTQSINKKVKTYSLGTKQKLALLLTLVTEPDILILDEPTNGLD
                     IESSQIVLAVLKKLALHENVGILISSHKLEDIEEICERVLFLENGLLTFQKVGKDSHN
                     FLFEIAFSSATDRDIFITKQEFWDIV"
     gene            4143..4623
                     /locus_tag="VVL45_001903"
                     /pseudo
     CDS             4143..4623
                     /locus_tag="VVL45_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5008..5217
                     /locus_tag="VVL45_001904"
     CDS             5008..5217
                     /locus_tag="VVL45_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU4188032.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            5207..5695
                     /locus_tag="VVL45_001905"
     CDS             5207..5695
                     /locus_tag="VVL45_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188033.1"
                     /translation="MKNRFYYSQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYGRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYIIYLPWIFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            5811..7271
                     /locus_tag="VVL45_001906"
     CDS             5811..7271
                     /locus_tag="VVL45_001906"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEU4188034.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            7268..8092
                     /gene="nadE"
                     /locus_tag="VVL45_001907"
     CDS             7268..8092
                     /gene="nadE"
                     /locus_tag="VVL45_001907"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEU4188035.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            8442..8993
                     /locus_tag="VVL45_001908"
     CDS             8442..8993
                     /locus_tag="VVL45_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4188036.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDVEITRNSICNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGNGMMTETLKAILDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
BASE COUNT         2928 a         1576 c         1994 g         2860 t
ORIGIN      
        1 ttttatatca gaataataaa taaaaaagcg atattctaga ctgtatcaat ccttttccat
       61 tataggagca acagagtggg agtagtcatc taaggactaa tttatgtatt tttacgagtc
      121 agtatcttgg gatactggtt tttacttttc tagacttttt gactacttgt taaaactggg
      181 ataattttcg actgtttaac agttattatg caaagtctaa aagattagaa ttgtcaaaac
      241 aatccgtcta ggcttgattt tatcctttat ttactataaa atgagaagga aaaatgtcaa
      301 acttttatat tgcaaatagg agaaatcatg acaaaaacat taaaacgtcc tgaggtttta
      361 tcacctgcag ggactttaga gaagctaaag gtagctgttc agtatggagc agatgctgtc
      421 tttatcggtg gtcaggccta tggtcttcgt agccgtgcgg gaaactttac tttcgaacag
      481 atggaagaag gcgtgcagtt tgcggccaag tatggtgcca aggtctatgt agcggctaat
      541 atggttatgc acgaaggaaa tgaagctggt gctggtgagt ggttccgtaa actgcgtgat
      601 atcgggattg cagcagttat cgtatctgac ccagccttga ttatgattgc agtgactgaa
      661 gcaccaggcc ttgaaatcca cctttctacc caagccagtg ccactaacta tgaaaccctt
      721 gagttctgga aagagctagg cttgactcgt gtcgttttag cgcgtgaggt ttcaatggaa
      781 gaattagctg agatccgcaa acgtacagat gttgaaattg aagcctttgt ccatggagct
      841 atgtgtattt catactctgg acgttgtact ctttcaaacc acatgagtat gcgtgatgcc
      901 aaccgtggtg gatgttctca gtcatgccgt tggaaatacg acctttacga tatgccattt
      961 gggaaagaac gtaagagttt gcagggtgag attccagaag aattttcaat gtcagccgtt
     1021 gacatgtcta tgattgacca cattccagat atgattgaaa atggtgtgga cagtctaaaa
     1081 atcgaaggac gtatgaagtc tattcactac gtatcaacag taaccaactg ctacaaggcg
     1141 gctgtggatg cctatcttga aagtcctgaa aagtttgaag ctatcaaaca agacttggtg
     1201 gacgagatgt ggaaggttgc ccaacgtgaa ctggctacag gattttacta tggtacacca
     1261 tctgaaaatg agcagttgtt tggtgctcgc cgtaaaattc ctgagtacaa gtttgtcgct
     1321 gaagtggttt cttatgatga tgcggcacaa acagcaacaa ttcgtcaacg aaatgtcatt
     1381 aacgaagggg accaagttga gttttatggt ccaggtttcc gtcattttga aacctatatt
     1441 gaagatttgc atgatgccaa aggcaataaa atcgaccgcg ctccaaatcc aatggaacta
     1501 ttgactatta aggtgcctca acccgttcaa tcaggagaca tggttcgtgc attaaaagaa
     1561 ggactcatca atctttataa ggaagatgga accagcgtca cagttcgagc ttaagaaagg
     1621 aaaaggaaat gatagaggca cagggtttct tagtggataa gcaaacaaga tgcattcatt
     1681 accatagcaa gctggatatt attgctttac aatgctatga ttgtaaaaag tattatgctt
     1741 gttatcggtg tcatgattca ttagaacatc acccttttga gccgtatccc ttatctttga
     1801 tacaggataa gcctatttta tgtggtgttt gtctaaaact actaacatat aagcaatata
     1861 aagaaagctt aagttgcccc ttttgttttt ctcgctttaa tccaggttgc caaaatcata
     1921 aggaacgcta ttttaaatag caaatcatct agttttgaag taggagaaaa ctcaatttca
     1981 agagaaaatg aagtaaatct tcccacaata aaacgcataa tatcaagatt gttcaatacc
     2041 tgatactatg cgtttttaag attttaaaga cttttttcct ttatctggta ttttgactac
     2101 ttgttaaaac tgggttaatt ttcgactgtt taatagttat tatgcaaagt ctaaaaggtt
     2161 agaattgtca aaacaatccg tctagagtat gcgtgatgcc aaccgtggtg gatgttctca
     2221 gtcatgccgt tggaagtacg acctttacga tatgccattt gggaaagaac gtaagagttt
     2281 gcagggtgag attccagaag aattttcaat gtcagccgtt gatatgtcta tgattgacca
     2341 tatctcagat atgattgaaa atggtgtgga cagtctaaaa atcgaaggac gtatggagtc
     2401 tattcactat gtatcaacag taaccaactg ctacaaggcg gctgtggatg cctatcttga
     2461 aagtcctgaa aagtttgaag ctatcaaaca agacttggtg gacgagatgt ggaaggttgc
     2521 ccaacgtgaa ctggctacag gattttacta tggtacacca tctgaaaatg agcagttgtt
     2581 tggtgctcgt cgtaaaatcc ctgagtacaa gtttgtcgct gaagtggttt cttatgatga
     2641 tgcggcacaa acagcaacta ttcgtcaacg aaacgtcatt aacgaagggg accaagttga
     2701 gttttatggt ccaggtttcc gtcattttga aacctatatt gaagatttgc atgatgctaa
     2761 aggcaataaa atcgaccgcg ctccaaatcc aatggaacta ttgactatta aagtcccaca
     2821 acctgttcaa tcaggagaca tggttcgagc tcttaaagag gggcttatca atctttataa
     2881 ggaagatgga accagcgtca cagttcgtgc ttaatgtagt tgtttagttt taaaaaacta
     2941 tgcaaagctc catatacaac acttaaacga gattaaagaa tggcgaaatc ccttgatgcg
     3001 caagagatta gctgtctttt ttatttttta agtgataaag tcggagttta ggcatcaaag
     3061 cctatcaaat taaacaaaga agcgatgtct tagatatttt gaaaaaaatt aataagcaga
     3121 aaactctcta ttattttgtt gtagagagtt ttttgttaat aaaatttcac aaaatgacat
     3181 ttatatattg cattaagtta gatatatgat ataatattgt taaaaagagg cgcaactttt
     3241 taaaattaat gagaatcaaa gagaaaacca ataatattaa tggaggaata aaaaatgtaa
     3301 gtaagcatta tggtcattca atcattctca aagatataaa ttttgcactt aacaagggtg
     3361 aaattgttgg tctagcaggg agaaatggag ttggtaagag tacgttgatg aaaattcttg
     3421 ttcagaataa tcaaccgact tcaggtaata ttataagcag tgataatgtt gggtatttaa
     3481 tcgaagaacc aaaattattt ttatctaaaa caggtttaga gaatttaaaa tatttgtcaa
     3541 atttatatgg tgttgactac aatcaagaaa gatttagatg tttgatccaa gagttagatt
     3601 tgactcagtc tattaataaa aaagtaaaga cctattcttt gggtacaaaa caaaaattag
     3661 ctttgcttct aactctcgtt acggaacctg atatattgat tttagatgaa ccgactaatg
     3721 gtttagatat tgaatcatca caaatagttt tagcggttct aaaaaaatta gctttacatg
     3781 aaaatgtggg aattttaata tcgagtcata aattagaaga cattgaagaa atttgtgaga
     3841 gagttctttt cttggagaac gggcttttga catttcaaaa agtaggaaaa gatagtcata
     3901 atttcttgtt tgagatagct ttttcatcag ctacagatag agacattttc attaccaaac
     3961 aagaattttg ggatattgtt taggaagagg gattgagaat tactatgtct gggaatattc
     4021 aaaatagtga gctttttaaa ttttttaacg aaaactctat taaagtagtt gattttgaaa
     4081 ctaaaaaaga gacgcttaaa gatatttacc taaatcgttc aaaataaagg aaggttataa
     4141 tcatgaaatt aaataaacag aagaatcgga tgatttacgt cttgtctaat tttctatatg
     4201 ctatctcagt ttccattatt tatgctttga atggcattgt gttactagtc atagtaagta
     4261 aattgggtat tccaggtgat ttaggattaa attttatagt agctattgta gtcaatacaa
     4321 ttttgttagt cctgttttat tttctattat cttacatttt ctatttatac aaattgaaaa
     4381 gtggcttggt atttggtatt ttagtagctt tactactctt tatctctaat atattaaata
     4441 cgatgatgat gaatactagt aatgatttgt ttatcaaagc aattgaactt cttccttttt
     4501 tattctttac ctgtatttgt ggcttcaaat acgatgtcta ttagtcagta tcttgtgcta
     4561 atcactacaa tcattttatt gtactttttc actctcaaga aaagcaagaa gtattcattt
     4621 tagtttcatt tagtattatt ttgcatacct aaaatacagt aaaaaatcag tcatcttggt
     4681 atgctcctgc tttcactatt caacacgttt ttgacttata ctaggctcat ttccaaaagc
     4741 attatataat agtgatatga aaccaactaa actaaacaag aaatataagc aataaaaatt
     4801 cgtttaaaag atcttactaa agctaatact aaataaaaat aaaagagtaa actaggaagt
     4861 ttatttcaaa caacctaaaa tactgatttt cggctgaaga taatactgga gtgcaaatta
     4921 atggggttat aataaatagc tgatagcttg tgttggtttt ggatttttta agagtagatg
     4981 agtattaaaa ctataaggag gacgaaggtg gctaaaaatt taaaattaaa attagctcgg
     5041 gtagagcgtg atttaacaca aggtcaactg gcagaggctg tcggggtgac acgccagact
     5101 attggtttaa tagaggcggg aaaatacaat cccagtctct cgctctgcca gtctatttgc
     5161 agatgtttag ggaaaaccct agaccaacta ttttgggagg aagaagatga aaaatagatt
     5221 ttattattct caattactag acgaaagaga agaacaactg ttcaataaag cgggctctga
     5281 aagtttctat atctgcattg ctttgtcgct cctatcttat atcatttcag tattagcacc
     5341 aagccttttt aattctaata tgctgctaat cgttatcatc atagggacat tttacttttt
     5401 caatcgtgcc cgttatctgg gagtgaccta ctatggtcgt tttcatttta cgattttggg
     5461 ttgttttttc ctaaccttgg ctattacggc tcttttgatg ttgcagaatt atcaattcaa
     5521 catagaaatt tatcagcaca atcctttgaa ttttaaatac ctgtctgctt gggtcattac
     5581 ttatatcatt taccttccgt ggatctttat tggcaatctt ggtcttaaga gctatggcga
     5641 atgggctcag aaaaaatttg aacaagatat ggatgaattg gagagtggag aatagcttgt
     5701 tactcttttc tcaatccagc taaaatgtga tataatagta ctaatttatt ggaatacatg
     5761 aaagttcttg aaaattttca tgggtttcta gctaaggaag taggaaaagt atgtatccag
     5821 atgatagttt gacattgcac acggacttgt accagatcaa catgatgcag gtttactttg
     5881 accaagggat tcacaataag aaggcggtct ttgaggtgta tttccgccaa cagcctttta
     5941 agaacggcta tgcggttttt gcaggtttgg aaagaattgt gaactatctt gaagacttgc
     6001 gtttttcaga tagtgatata gcctatttgg agtcgcttgg ttatcatggg gcgttcttgg
     6061 attaccttcg caatttcaag ttggagttga ccgttcgttc tgcccaagaa ggggatttgg
     6121 tttttgctaa tgaaccgatt gtgcaggtgg aaggacctct agcccaatgt cagttggtcg
     6181 aaacggctct tttgaacatc gtcaactacc agactttggt ggcgacgaag gcagctcgta
     6241 ttcgttcggt tatcgaagat gaacccttga tggagtttgg gacacgtcgg gctcaagaaa
     6301 tggatgcggc catctgggga acacgcgcag ctgtgattgg tggcgccaat ggaaccagca
     6361 acgtgcgtgc gggtaagctc tttgacattc ctgttttggg aacccatgcc catgccttgg
     6421 tacaggttta tggcaatgac tatgaagctt tcaaggctta cgctgcgacc cacaaaaatt
     6481 gtgtctttct tgtggatacc tatgacaccc ttcgcatcgg tgtaccagct gccattcagg
     6541 tggcgcgtga gctgggtgat cagattaact ttatgggtgt gcggattgac tctggggata
     6601 ttgcctacat ttctaagaaa gtccgtcagc aactggatga ggctggattt acagaggcta
     6661 agatttatgc ttctaatgat ctagatgaaa ataccatcct taacctcaag atgcaaaagg
     6721 ccaagattga tgtctggggt gtgggtacca agctgattac agcctatgac cagccggctc
     6781 ttggggcggt ttacaagatt gttgcaatcg aagatgaaac tggtcagatg cgcaatacga
     6841 ttaagctgtc taataatgct gaaaaagttt ctacgccagg taagaagcag gtgtggcgca
     6901 ttaccagtcg tgaaaaaggc aagtcagaag gcgactatat cacttatgat ggtgtggata
     6961 ttagcgacat gacagaaatc aagatgttcc atccgaccta tacatacatc aagaagacgg
     7021 ttcgtaattt tgatgccgtt cctctcttgg tggatatctt caaagaagga atattagttt
     7081 acaacttgcc tagtttgact gacattcagg attatgcccg taaggaattt gacaagttgt
     7141 gggatgagta taagcgtgtg ctcaatccgc agcactatcc agtggatttg gcgcgtgatg
     7201 tatggcaaga taagatggac ttgattgata agatgcgcaa ggaagccctt ggtgaaggag
     7261 aagaagaatg agtttgcaag aaacgattat ccaagagctg ggtgtcaaac cagtgattga
     7321 tgcccaggaa gaaatccgtc gttctattga tttcttaaaa agatatctga aaaaacatcc
     7381 cttcctaaaa acctttgtac tagggatttc tgggggacaa gactcaacct tggcaggacg
     7441 tttggcgcaa ttagctatgg aagaactgcg agctgaaacg ggagacgata gctacaaatt
     7501 tatcgctgtc cgcctgccat acggagtgca agctgatgaa gcagatgctc aaaaagccct
     7561 agccttcatc cagccagatg tcagcttggt tgtgaatatc aaggaatcag ctgatgccat
     7621 gacagctgca gttgaagcga caggtagtcc tgtttcagac ttcaacaagg ggaatatcaa
     7681 ggcacgttgc cgtatgattg ctcagtatgc ccttgctggt tcccatagcg gagcggtcat
     7741 tggaacagac cacgccgcgg aaaatatcac aggtttcttt accaagtttg gtgacggcgg
     7801 tgcggatatt ctccctcttt accgcctcaa taaacgccaa ggaaaacagc tcttgcagaa
     7861 acttggcgca gagccagccc tttatgaaaa aatcccaacg gcagacctag aagaagataa
     7921 accaggccta gctgacgaag tcgcacttgg agtcacctac gcagagattg acgactacct
     7981 agaaggcaaa acaatcagcc cagaagctca agcgaccatt gaaaactggt ggcacaaagg
     8041 ccaacacaaa cgccacttac ccatcaccgt atttgatgac ttttgggagt aaaaaggtcc
     8101 gggggacctt tttagcttct tgccctgaaa ttaaaaagca agaaaaacct ccactggagg
     8161 ttttcagcct ctcatcttga aataagaaag tgagagaagg tctgggggat cttgaacccc
     8221 gagtttagaa ataagaaaat gaggcagatt cagtaactcg aagagttcga tttcatcgtc
     8281 ttacccctgc aacgatgact aggtttgaaa aagcttgcta gagcgcattt caaaccaggc
     8341 agcaactgcg tcaagaaatt agaagacaaa ctcgttttct agctgttact gagttgagcc
     8401 tttttactac gagtatagaa ataaggaagt gaggtagcat catgaaatct atcggtacgc
     8461 aaatattaca gacagaacgt ttgattttaa gaagatttgt ggagagtgat gcagaagcca
     8521 tgtttcaaaa ttgggcttca tccgctgaga atctgaccta tgttacctgg gatccccatc
     8581 ctgatgtcga aatcactcga aactcgattt gcaattgggt tgcttcctat actaatctca
     8641 actattataa atgggccatt tgtctaaaag aaaacccaga gcaagtaata ggagatatca
     8701 gcattgttaa gatagacgag gctgatttaa gctgtgaaat tggctatgtg ttaggcaagg
     8761 cttactgggg aaatggtatg atgacagaga ctttgaaagc tatcttggac ttttgtttta
     8821 ctcaagcagg ttttcaaaag gtcagagcac gttatgccag tctcaaccca gcttcaggtc
     8881 gtgtcatgga aaaggctgga atgtcctatc tacaaaccat tgttaatggt gtagagagaa
     8941 aaggctatct tgcggatctt atttattatg gtataagtag ggaagaatgt tgaattctat
     9001 tttctgtttc tatcgaagtc aactatttat tgtaaatata ataattagca ttccaagttt
     9061 atttgaaact ttaaaatagc atattgatta gtacaagaca gatgttctag ttccttcttt
     9121 aatctggttt agtgttagtt aaaaaatcgc tttaagcttg taactaagag ggagctaatc
     9181 gactagattc tccagccgaa caggtggtaa tgtacttttt atagtgtaat cctagctgtt
     9241 gttaaattta aaatagaatc ctctatcgag ttagggaatt aaattcaacc aattttattc
     9301 atgttttttc tatcaaatta tctaatatta aaatagtctc attctgatga gaaaacta
//
LOCUS       DAVHEX010000074         8693 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVHEX010000074 DAVHEX010000000
VERSION     DAVHEX010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8693
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.075"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            58..921
                     /locus_tag="VVL45_001909"
     CDS             58..921
                     /locus_tag="VVL45_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU4188037.1"
                     /translation="MNTLAEKFRLKRKELRLSQQTLAEGICEQSQISKIERGHFIPSA
                     DLLFKLSQRLEVPLDYFFNEQIEVKSNLSNFKHLSARLLDDRNYDDLEYLYKIEVERS
                     TFLTLEDRTYLEWIKSIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFY
                     SLVGREQEYEANYSHLIELYQTKNLDHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEA
                     LETIELCKQRQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEARELCKIYNNPLMLMK
                     IENYLKELDTV"
     gene            1304..1924
                     /locus_tag="VVL45_001910"
     CDS             1304..1924
                     /locus_tag="VVL45_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188038.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            2010..2453
                     /gene="tsaE"
                     /locus_tag="VVL45_001911"
     CDS             2010..2453
                     /gene="tsaE"
                     /locus_tag="VVL45_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEU4188039.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            2443..2961
                     /locus_tag="VVL45_001912"
     CDS             2443..2961
                     /locus_tag="VVL45_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4188040.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            2969..3985
                     /locus_tag="VVL45_001913"
     CDS             2969..3985
                     /locus_tag="VVL45_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEU4188041.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNVERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSIGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNLLRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            4070..5326
                     /locus_tag="VVL45_001914"
     CDS             4070..5326
                     /locus_tag="VVL45_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEU4188042.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FTLRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGVSSIFKGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            5381..6547
                     /gene="recA"
                     /locus_tag="VVL45_001915"
     CDS             5381..6547
                     /gene="recA"
                     /locus_tag="VVL45_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEU4188043.1"
                     /translation="MAKKPKKLEEISKKFGSEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVPLDLGDELEIEIEE"
     gene            6853..8223
                     /locus_tag="VVL45_001916"
     CDS             6853..8223
                     /locus_tag="VVL45_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU4188044.1"
                     /translation="MNKKRTVDLIHGPILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSTFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
BASE COUNT         2646 a         1464 c         1903 g         2680 t
ORIGIN      
        1 ttttgaacta aaaactttat aatgaaatta agcaaatagg aaggtttatt atctttaatg
       61 aatacactcg cagagaaatt cagattgaaa agaaaagagt tgagactctc ccaacaaact
      121 cttgcagaag gaatttgtga acaaagccag attagtaaaa ttgaaagagg tcattttatt
      181 ccctccgcag acttgttatt caaactctct caacgacttg aagtcccttt ggattacttt
      241 ttcaatgaac aaatcgaagt taagtctaac ctctctaatt tcaaacatct atctgctcgc
      301 ctattagatg acagaaatta tgacgattta gaatatcttt ataaaataga agtagaacga
      361 agcacttttc taacattaga agaccgaact taccttgaat ggattaaatc tataattgac
      421 ttctatcaat atgacagtaa gtgtgaggca atttcgtcat tggaaaatat attattaaaa
      481 gtctcttcaa atactctgat ttatttaaag gcgttgaata ctctatctaa tttctattcc
      541 ttagtgggtc gtgaacaaga atatgaggca aactactctc atttaataga gttgtatcag
      601 acaaaaaatt tggaccatca agagttttta tttggctaca tcagagttcg ttacaactac
      661 gctcactacc tagtatcaaa ggaaaaatat aatgaagcca tccaagaagc tcttgaaacg
      721 attgaactct gtaaacaaag acagacaagc taccaattgg ctcctctact tattcttgta
      781 ggaaatgctg gagccaaatt tctagacaga gaacaagtca aaaattatta tatagaagca
      841 agagagttat gtaagattta taacaatcct ttaatgttga tgaagataga aaattatttg
      901 aaggaactag atactgttta gttaatcttg acattatagt ttttctgaaa cgttaaataa
      961 cctgtaaggc tgatagttag attaatacta tcagccttat aattatgtga ctctgagtcc
     1021 gtctcgctcc gtgaggtgcg agacaaaaca accacccgtt tgacggattt tcatgatttc
     1081 aaatttatat tcttcgaaaa tctcttcaaa tcacgtcagc tctatctgca atctcaaaac
     1141 actgttttga gcaacctgtg gctagctacc tagtttgatc tttgattttc attgagtatt
     1201 agtctctagt gtttgtttag cgtcttaaag agacatttga gaagattaga aacagttctc
     1261 tcttttctcc tagtataatg attggtagga agggagagag aagatgaaat caatgagaat
     1321 cttatttttg ttagctttaa ttcaaatcag tttgagtagc tgtttcctat ggaaggaatg
     1381 catcttgtcc tttaaacaaa gtacagcttt tttcatcgga agcatggttt tcgtttcagg
     1441 aatctgtgct ggagtaaatt atctttatac ccgtaagcaa gaagtccata gtgtcctagc
     1501 cagtaagaag tcggtgaagc ttttttacag tatgttactc ttaattaatt tgttaggagc
     1561 tgttcttgtt ttgtcagata acttgttcat caaaaatacg ctgcagcaag aattagttga
     1621 ctttttattg ccatccttct ttttcctatt tgggctagat ttgctgattt ttttaccctt
     1681 gaaaaaatac gtgcgcgatt ttcttgctat gctggacaga aaaaagacag tgttggtgac
     1741 tattttagca acacttcttt tcttaagaaa tccaatgacc attgtctcac ttctgattta
     1801 tattggactg ggcttgtttt ttgcagccta tcttgtccca aattcggtta agaaggaagt
     1861 ttccttttat ggtcatattt tccgagatct tgtattggtc attgttacgc tcattttctt
     1921 ttagagaaag cctgtttttt atggatgtta tgatgataag tttagtagga aaatagacac
     1981 aaaagaaaag tttttggtat aataagagta tgtacacaaa aaatgaagaa gagttgcaag
     2041 ccttagggga gcgtttgggc catctattag caaagaatga tgttttaatc ttaactggag
     2101 aactgggtgc aggtaaaacg acctttacta aaggacttgc aaaaggatta cagatttctc
     2161 aaatgattaa aagtcccacc tatactatcg tgagagagta tgaaggtcga cttccacttt
     2221 atcacctaga tgtttatcgt attgaaggag atgctgattc tatcgacttg gatgagttta
     2281 tctttggtgg cggcgtgact gttattgagt ggggaaatct cttaggagat gccttgccag
     2341 atgcttattt agaattggaa attctaaaag aagcagatgg acgccgttta aattttcagg
     2401 caaagggttt gcgtgctgag aaattgttag aggagcttca atatggagta tgaattgctc
     2461 attagggaag cagagcccaa agatgcagct gaattagtgg cctttttaaa tcgtgtgagt
     2521 ttggagacag actttaccag cctagacgga gatggtattc tcttgactag tgaggagatg
     2581 gaaatattcc tcaacaagca agctagttcg gacaatcaga taaccttact tgcattctta
     2641 aatggcaaaa ttgctggtat tgtaaatatt acagctgatc agcgtaagag agtccgtcat
     2701 attggagatc tcttcattgt gattggaaaa agatattgga ataatggctt gggaagtttg
     2761 ttgctagaag aagcgataga gtgggcacaa gcaagtggca ttctgcgtcg tctccaactg
     2821 actgtccaaa ctcgtaatca agcagcagtc catctttatc aaaagcatgg ctttgtcatt
     2881 gaaggtagcc aagagcgtgg ggcatatata gaagaaggga aatttatcga tgtttacttg
     2941 atgggtaaac tgataggtta gtagaaatat ggttaaaaaa attattggaa tggtgctagc
     3001 tttactttct gtaactgtag taggagtagg tgtttttgct tatactattt atcaacaagg
     3061 gacagaaacc ttagctaaaa cctataaaaa aatcggtgaa gaaaccaagg ttattgaagc
     3121 gactgaacct ctaaccattc tgttaatggg agtggacacc ggaaatgttg aacgaactga
     3181 aacttgggtc ggtagaagtg atagcatgat cttgatgaca gtgaatccta aaacgaaaaa
     3241 aacaacaatg atgagtttag agcgggatat tctgacgcgc attgaatcag ggaatggtca
     3301 ggctcatgaa gcgaaactga actcagcata tgcagatggt ggagcagagc ttgctataga
     3361 aaccattcaa aaaatgatga atatccatat tgatcgctat gtgatggtca atatgagagg
     3421 attgcaaaaa ctagtggatg cagtaggagg tattacagtc aataatatcc taggtttccc
     3481 aatttctatc agtgaccaag aagaatttaa tactatttct atcggtgttg gggagcaaca
     3541 tattggggga gaagaagccc tagtctatgc acgaatgcgt taccaagatc ctgaggggga
     3601 ttatggtcgt caaaaacgtc aacgtgaagt tattcaaaaa gtcatggaaa aagctctcag
     3661 tttaaatagc attggtcatt atcaagagat tctaaaagct ttgagtgaca atatgcagac
     3721 caatattgat ttgtctgcaa aaagtatccc taacttgcta ggctataaag attcatttaa
     3781 aaccattgaa actcagcagt tgcagggtga aggagagata cttcaaggtg tttcttacca
     3841 gattgtttcg agagcacata tgttggaaat gcaaaatcta ctccgacgtt ctttgggaca
     3901 agaagaagtt actcagcttg aaaccaatgc ggttttattt gaagatttat ttggcagagc
     3961 acctgttggt gatgaagata attaactttt gatacaaata aaaaaatcaa tcgtaggaaa
     4021 attcctgcga ttttttcaaa aaaatacgaa tagataggta ggaggaaaca tgaaagcaga
     4081 aatcattgct gttggaacag agattttgac agggcagatt gtcaacacca atgcccagtt
     4141 tttgtcggaa aaactagctg agattggggt agatgtatat tttcagacgg ctgtaggaga
     4201 caatgaagtt cgtctcttgt ctttgcttga gattgccagt caacgtagca gtctggtgat
     4261 tttgacaggc ggtttggggc caactgagga cgacctaacc aaacaaaccc tagctaaatt
     4321 tttagggaaa gcattagtct ttgatcctca ggctcaggag aagttggata tcttttttac
     4381 cctgcgacca gactatgccc gaacaccgaa taacgaaaga caagctcaaa ttgtagaagg
     4441 agcgattcca ctgccaaacg aaacaggact ggctgttgga ggaaaattag aagtagacgg
     4501 agtgacctat gtcgtccttc caggtccgcc aagtgaattg aaacccatgg tcttaaacca
     4561 acttctaccc aagttgatga cagggagcaa gctgtattcc cgagttcttc gtttctttgg
     4621 gattggcgag agccagttgg ttacgatttt ggctgattta attgataatc agatcgatcc
     4681 taccttggcc ccttatgcca agacaggaga agtcactcta cgtctgtcaa caaaggctag
     4741 cagtcaagaa gaggcgaatc aagcgctgga tatcttggaa aatcaaatct tggactgcca
     4801 gactttcgaa ggaatttctt tacgagactt ttgctatggt tatggggaag aaactagttt
     4861 agccagcatt gtggtagaag aactgaaaag gcaagggaaa accatcgcgg ctgcagagag
     4921 tttgacggca ggtcttttcc aagctaccgt ggcgaatttt tctggagttt caagtatatt
     4981 taagggtggt tttgtgacct atagcttgga ggaaaaatca aggatgttgg atattcctgc
     5041 caagaatttg gaagaacatg gtgtggtgtc tgaatttaca gctcagaaga tggctgagca
     5101 ggcacgaagc aagacccagt ctgattttgg aattagtttg actggagtgg caggaccaga
     5161 tagcctagaa ggacacccag ttgggacagt cttcataggc ttggcgcaag atcaaggaac
     5221 tgaggttatc aaggttaata ttggaggcag aagccgagca gatgtacgtc acattgcggt
     5281 tatgcatgcc tttaacctag ttcgcaaggc tttattaagt gactaacttt tgatataata
     5341 gtagataggt ctgaggatca ttagaatgta ggagaataga atggcgaaaa aaccaaaaaa
     5401 attagaagaa atttcaaaaa aatttgggtc agaacgtgaa aaggccttga atgacgctct
     5461 taaattgatt gagaaagact ttggtaaagg atcaatcatg cgtttgggtg aacgtgcgga
     5521 gcaaaaggtg caagtgatga gctcaggttc tttagctctt gacattgccc ttggctcagg
     5581 tggttatcct aagggacgta tcatcgaaat ctatggccca gagtcatctg gtaagacaac
     5641 ggttgccctt catgcagttg cacaagcgca aaaagaaggt gggattgctg cctttatcga
     5701 tgcggaacat gcccttgatc cagcttatgc tgcggccctt ggtgtcaata ttgacgaatt
     5761 gctcttgtct caaccagact caggagagca aggtcttgag attgcgggaa aattgattga
     5821 ctcaggtgca gttgatcttg tcgtagtcga ctcagttgct gcccttgttc ctcgtgcgga
     5881 aattgatgga gatatcggag atagccatgt tggtttgcag gctcgtatga tgagccaggc
     5941 catgcgtaaa cttggcgcct ctatcaataa aaccaaaaca attgccattt ttatcaacca
     6001 attgcgtgaa aaagttggag tgatgtttgg aaatccagaa acaacaccgg gcggacgtgc
     6061 tttgaaattc tatgcttcag tccgcttgga tgttcgtggt aatacacaaa ttaagggaac
     6121 tggtgaccaa aaagaaacca atgtcggtaa agaaactaag attaaggttg taaaaaataa
     6181 ggtagctcca ccgtttaagg aagccgtagt tgaaattatg tacggagaag gaatttctaa
     6241 gactggtgag cttttgaaga ttgcaagcga tttggatatt atcaaaaaag caggggcttg
     6301 gtattcttac aaagatgaaa aaattgggca aggttctgag aatgctaaga aatacttggc
     6361 agagcaccca gaaatctttg atgaaattga taagcaagtc cgttctaaat ttggcttgat
     6421 tgatggagaa gaagtttcag aacaagatac tgaaaacaaa aaagatgagc caaagaaaga
     6481 agaagcagtg aatgaagaag ttccgcttga cttaggcgat gaacttgaaa tcgaaattga
     6541 agaataagct gttaaagcag tggagaaatc cgctactttt tcgatttttg attcaagttt
     6601 ttagattata tatagtagct tgaaataaga tatgaacaac tctattagga aagtcaaatt
     6661 aatttctaga aatgttttag cagctacagc gtactattcc aaactcaacc aactataata
     6721 gatcgaaact agaatagtac atatctactt ctaaaacatt gttaaaaatc gatttgactt
     6781 tccttatttc attccgctat atatagtttg ctgtttcttg tcgctcctct ggaaagctga
     6841 tataatagct ttatgaataa aaaacgaaca gtggacctga tacatggtcc gattcttccc
     6901 tcgctcttaa gcttcacctt tccaattttg ctatcaaata tttttcaaca gctctataac
     6961 actgctgatg tcttgattgt tggacgattt cttggtcaag aatccttggc tgcagtagga
     7021 gcgacgacag cgatttttga cctgattgta ggttttacac ttggtgttgg caatggcatg
     7081 gggattgtca ttgctcgtta ttatggggct cggaatttca ctaaaatcaa ggaagcagta
     7141 gcagccacct ggattttagg tgctcttttg agcattctag ttatgttgct gggctttctt
     7201 ggcttgtatc ctctcttgca atacttagat actcctgcag aaattcttcc tcaatcttat
     7261 caatatattt ctatgattgt gacctgtgta ggtgtcagct ttgcttataa tctttttgca
     7321 ggcttgttgc ggtctattgg tgacagtcta gcagccctgg gatttctgat tttctctgcc
     7381 ttggttaatg tggttctgga tctctatttt attacgcaat tgcatctggg agttcaatcc
     7441 gcaggacttg ctaccattat ttcgcaaggt ttatcagcgg ttctctgctt ttattatatt
     7501 cgtaaaagtg tgccagaact cttgccacag tttaaacatt tcaaatggga caaaagcttg
     7561 tacgcggatc tcttggagca aggtttggct atgggcttga tgagttcaat tgtatctatc
     7621 ggcagtgtga ttttacagtt ttctgttaat acatttggtg cagtgattat tagtgcccag
     7681 acggcagctc gacgcattat gacctttgcc cttcttccta tgaccgctat ttctgcatca
     7741 atgacgacct ttgcttctca gaatctagga gctaagcgac ctgaccgtat tgttcaaggt
     7801 cttcgaatcg gcagtcgttt aagtatatcc tgggcagttt ttgtttgtat tttcctcttt
     7861 tttgccagtc cagctttggt ttccttcttg gctagttcga cagatggtta cttgatagaa
     7921 aatggaagtc tctatctgca aatcagttca accttttatc ccattttgag cctcttgttg
     7981 atttatcgca attgcttgca gggcttgggg caaaagatcc ttcctctagt ttctagcttt
     8041 attgaactaa tcggaaaaat cgtttttgtg gttttgatta ttccttgggc aggatataag
     8101 ggtgttatcc tttgtgaacc tcttatctgg gttgccatga cagttcaact gtacttctca
     8161 ttattccgtc atcccttgat aaaagaaggc aaggcaatct tggcaaccaa agtgcaatcc
     8221 tagttggatt tactgaataa aatccatttc ctctagtgaa aatcgaaaaa acttgtgttc
     8281 tcttctttag tttggtgttg aaaatagttt aacagacttt tgacttcttt tatatgatat
     8341 aataaagtat agtatttatg aaaaggacat atagagactg taaaaatata cttttgaaaa
     8401 tctttttagt ctggggtgtt attgtagata gaatgcagac cttgtcagtc ctatttacag
     8461 tgtcaaaata gtgcgttttg aagttctatc tacaagccta atcgtgacta agattgtctt
     8521 ctttgtaagg tagaaataaa ggagtttctg gttctggatt gtaaaaaatg agttgtttta
     8581 attgataagg agtagaatat ggaaattaat gtgagtaaat taagaacaga tttgcctcaa
     8641 gtcggcgtgc aaccatatag gcaagtacac gcacactcaa ctgggaatcc gca
//
LOCUS       DAVHEX010000075         8502 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVHEX010000075 DAVHEX010000000
VERSION     DAVHEX010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8502
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.076"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..230
                     /locus_tag="VVL45_001917"
     CDS             <1..230
                     /locus_tag="VVL45_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HEU4188045.1"
                     /translation="EQSGCLDMVVIQKQNEIEELSINMEKKIMNYIFEDDGNVSEVIS
                     ALDIIHHLDKIAHTTQAIYKWIMYRKYGNIN"
     gene            243..1718
                     /gene="galT"
                     /locus_tag="VVL45_001918"
     CDS             243..1718
                     /gene="galT"
                     /locus_tag="VVL45_001918"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEU4188046.1"
                     /translation="MKTIIDNFVDRVIEYGMYNEIDKIYVKNRVLALIGEEGIDRISD
                     ENDLKQIKDYLVEIALKNGKIKDLIEEKECLGAELMNFIVPLPSRLNDIFWSSYDISP
                     QEAVEEFYKLSKDSDYIKTSAIAKNIEFRASTKYGELEITINLSKPEKDPKTIAAEKL
                     VKATNYPKCLLCMENEGYQGRINYPARSNHRIIRLKLGDEVWGFQYSPYSYFNEHAIF
                     LNSQHVPMAITSKTFEQLLEIVDILPGYFAGSNSDLPISGGSILSHNHYQGGKHIFPM
                     EKAKFESEFCFKDFEDVNAGIVKWPMSVIRLQSENKNRLLDLATKILNKWREYSDLEV
                     DIIAMTEDVPHHTVTPIARKVDGRYELDIVLRDNHTTEQYPDGVFHPHQDVQHIKKEN
                     IGLIEVMGLAILPPRLKPELEEVGKYLLGEDNAIADYHLEWADQLKEKYPRINKEEVN
                     SVVQHEAGQVFARVLEDAGVYKNTPSGHEAFMRFVKSVGIN"
     gene            1731..2741
                     /gene="galE"
                     /locus_tag="VVL45_001919"
     CDS             1731..2741
                     /gene="galE"
                     /locus_tag="VVL45_001919"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU4188047.1"
                     /translation="MAILVTGGAGYIGSHTVVELLNLGKEVIIVDNLSNSSKLVLDRI
                     EAITGIRPVFYELDVCDKQALRKVFEQESIDAAIHFAGYKAVGESVQKPVMYYKNNIM
                     STLALVEVMSEFNVKKIVFSSSATVYGINNQSPLIETMQTSATNPYGYTKVMLEQILK
                     DVHVADSEWSIALLRYFNPIGAHESGLIGEDPSGIPNNLMPYIAQVAVGKLSELSVFG
                     NDYDTLDGTGVRDYIHVVDLAIGHIKALEKVSEKTDVYIYNLGSGEGTSVLQLVNTFE
                     SVNKIPIPYKIVPRRSGDVATYYANADKAYKELNWRTTKSIEDMCRDTWNWQSKNPNG
                     YN"
     gene            2905..3564
                     /locus_tag="VVL45_001920"
     CDS             2905..3564
                     /locus_tag="VVL45_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HEU4188048.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            3589..4656
                     /locus_tag="VVL45_001921"
     CDS             3589..4656
                     /locus_tag="VVL45_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU4188049.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSEKGWEVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVK"
     gene            4760..5770
                     /locus_tag="VVL45_001922"
     CDS             4760..5770
                     /locus_tag="VVL45_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4188050.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            5782..7473
                     /locus_tag="VVL45_001923"
     CDS             5782..7473
                     /locus_tag="VVL45_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU4188051.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDSNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            7476..8186
                     /locus_tag="VVL45_001924"
     CDS             7476..8186
                     /locus_tag="VVL45_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEU4188052.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
     gene            8282..>8502
                     /locus_tag="VVL45_001925"
     CDS             8282..>8502
                     /locus_tag="VVL45_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188053.1"
                     /translation="MKDYKINFDLGKIEYFDNNCLIQVYKFISFYDICEMVFAFHLPP
                     DELITNVIFKEKINSMLKCYIDRLLYVFI"
BASE COUNT         2888 a         1165 c         1662 g         2787 t
ORIGIN      
        1 aggaacaatc tggatgttta gatatggttg ttatacaaaa acagaatgag atagaagagc
       61 ttagtataaa tatggaaaag aaaataatga attacatatt tgaagatgat gggaacgttt
      121 ctgaagtaat tagtgcttta gatattattc atcaccttga taaaattgct catactacgc
      181 aagcaattta taaatggata atgtatagaa aatatgggaa cataaattag aaagaaaaat
      241 atatgaaaac aattattgat aattttgttg atagagtaat tgaatacgga atgtataatg
      301 aaattgacaa aatatatgtg aaaaatagag tactagcgtt aataggtgag gagggaattg
      361 atcgcatttc cgatgaaaat gacctgaaac aaataaaaga ttacttagtg gaaatcgcct
      421 taaaaaacgg taaaattaaa gatttaatcg aggaaaagga atgtttagga gcagagttaa
      481 tgaatttcat agttccttta cctagtcgat taaatgatat tttttggagt tcatatgata
      541 tatcacctca agaagcagtt gaagagtttt ataagttaag taaagatagt gattatatta
      601 aaacttctgc tattgctaag aatattgagt ttcgagcatc aactaaatac ggagagctag
      661 agattacgat aaatttgtca aaacccgaaa aagatccaaa aacaattgct gctgaaaaat
      721 tagtaaaagc aactaattat cccaaatgtt tactatgtat ggaaaatgaa ggatatcagg
      781 ggcgaataaa ttatccagca cgttcgaatc atagaattat tcggttgaaa cttggtgacg
      841 aagtatgggg attccagtat tctccctact catactttaa tgagcatgcg atatttttga
      901 atagtcaaca tgtaccaatg gctattacct ctaaaacatt tgaacaatta ttggagattg
      961 ttgatatttt gccaggatat tttgctggtt caaattctga tcttcctatc tcaggaggct
     1021 ccattcttag tcataaccac taccagggag ggaaacatat tttcccaatg gaaaaagcaa
     1081 aatttgagag tgaattttgt tttaaagact ttgaggatgt taatgctggt attgtaaaat
     1141 ggccaatgtc agtaattagg ttacaaagtg aaaataaaaa tcgcttgtta gatttagcta
     1201 ctaaaattct aaataagtgg agagaatatt cagatttaga agtagacatc attgctatga
     1261 ctgaagatgt cccacaccat acggtgactc ctattgctcg taaagtggac gggagatacg
     1321 aattagatat tgttctaaga gataatcata ctactgaaca atacccagat ggtgttttcc
     1381 atcctcatca agatgtacaa catattaaaa aggaaaatat cgggttaatc gaagtgatgg
     1441 gacttgcgat attaccacct cgtttgaaac cagagttaga agaagttgga aaatatcttt
     1501 taggagagga taatgcaatt gcggattatc atttagaatg ggccgaccaa ttgaaagaaa
     1561 aatatcctcg cattaataaa gaagaggtta atagcgtagt tcaacacgaa gcaggacaag
     1621 tgtttgcgag agttctggaa gatgcaggag tttataagaa tacaccgtct gggcatgaag
     1681 cgtttatgag atttgttaag tcggttggaa ttaattagga ggaaacccag atggcaatat
     1741 tggtaacagg cggagctggt tatattggta gccataccgt agtagaatta ctaaatttag
     1801 gaaaggaagt tatcattgtc gacaaccttt cgaactctag caaattggtg ctagaccgta
     1861 ttgaagcaat tacaggaata cgtcccgtgt tttacgaatt agatgtttgt gataaacaag
     1921 cattgagaaa ggtatttgaa caagaatcga ttgatgctgc aattcatttt gcaggttata
     1981 aagctgtcgg agaatccgtg caaaagcctg tgatgtacta caaaaataat attatgagta
     2041 cattggcact tgttgaagtg atgtcagaat ttaatgttaa aaagattgtc ttttcttcaa
     2101 gtgcgactgt atatggaatt aacaatcagt cacctctaat tgagacgatg caaacaagtg
     2161 cgacaaatcc ttatgggtat acgaaagtga tgcttgagca aattttaaaa gatgttcatg
     2221 tggcagattc agaatggagt attgcgttgc ttcgttattt caatccaatt ggtgctcatg
     2281 agtctggttt gattggggaa gatccctcag gaattcctaa caacttgatg ccttatattg
     2341 cacaagtagc ggtaggtaag ctatcagagc taagtgtgtt cggaaatgat tatgatacgc
     2401 ttgatggtac tggtgtgcgt gattatatcc atgtagtaga tttagcaata ggacatataa
     2461 aagctttaga aaaagtatct gaaaaaacag atgtttatat ctataaccta ggctcgggag
     2521 aaggcactag tgtattacaa cttgtaaata catttgaaag tgttaataag atccctattc
     2581 cttataaaat agttccaaga cgttcaggag acgttgcaac ttattatgca aatgcagaca
     2641 aagcatataa ggaattaaat tggaggacaa caaaatcgat tgaagacatg tgtagagata
     2701 catggaattg gcaatcaaaa aatcccaatg gctataattg atgatactgt ctatatgagt
     2761 gctgtatttt ggaaaaatta cttaaccttt taatgaaaga taaattaaaa aaaatgtttg
     2821 acagcgcttg acaaaggaaa cggtttcatt gtataatgta aacacaatga caccgatgtc
     2881 aaatataaaa aagaaggtct ttatatgaaa aacttaacaa aaattaagtt caaagaaaat
     2941 ggggaattta atcatttccc tgggaataca gttgtagcaa atctttatac taaacaagat
     3001 ttgatggaag ttgttgatat tattcaatca cgttatagag aattaccatt tatcgataag
     3061 tttactttaa ctccaaggaa ttctattcac atgactgtaa ttgaattgtt gtgccatgaa
     3121 aatcgtgaaa cggagttttg gagcagtaat cttcctctag atacaccttt acaggaaata
     3181 catgattact ttgctaaaca acttgaaatt tttccattgt tggatgaaga aattcatatg
     3241 cgtataactg aaatgggaaa acaaaacata ctagttgaac ctgcagatga agcttctgca
     3301 aagagattag aagaaattcg tacttatgtc tcagaaaaag caggtgtttg tttccctaat
     3361 catgatagat atcaattcca tatttcaatt gggtatcttc ggattcctct aaccgaagag
     3421 gaggaagaag agtttactaa agtcagagca gaattaactg aaattttatt agagaagatt
     3481 ccaactatta ctgtaaaccg tattgactat actgtatttg aagatatgag acaatttgtt
     3541 ccatatcacg aaaaatttaa ataaaataat aaaaaaggag aacttatcat gaaaaaaatg
     3601 aaagtttggt ctactgtact tgcaacggga gttgctctta ctacacttgc tgcttgctct
     3661 ggaggttcaa attctacgac tgcttcttca tctgaagaaa aagctgataa aagtcaagaa
     3721 ttagttatct attcgaactc agtctcaaat ggtcgtggtg attggttaac tgctaaagca
     3781 aaagaagctg gttttaatat aaaaatggtt gatatcgctg gcgctcaatt agcagaccgt
     3841 gttattgctg agaagaataa tgcagttgca gatatggtat ttggaattgg tgctgttgac
     3901 tcaaataaaa ttagagatca aaaattacta gtacagtaca agcctaaatg gttagataaa
     3961 attgatcaat ctttatcaga taaagataat tattataatc ctgtgattgt tcaaccatta
     4021 gttttaattg gggcgcctga tgtaaaagaa atgcctaaag attggactga attaggtagt
     4081 aagtataaag gtaaatattc aatttatggt cttcaaggtg gtacaggacg ggcaattcta
     4141 gcaagtatct tagttcgtta ccttgatgaa aaaggagatt taggcgtatc agaaaaagga
     4201 tgggaagttg caaaagaata ctttgcaaat gcctatactc ttcaaaaagg tgaaagctca
     4261 gttgttaaag cgttagataa agaaagccca attcaatacg gtatgatctg gggttctgga
     4321 gcgttaattg gacaaaaaga acaaaatgtt gaattcaaag ttatgactcc tgagattggt
     4381 gtaccatttg taactgaaca aactatggtt ttaagcacta gtaaaaaaca agcgttagct
     4441 aaagaattta ttgattggtt tggtcaatca gaaattcaag tagaatatag taaaaaattt
     4501 ggatctattc ctgccaataa agatgcccta aaagagttac ctgaagatac gaaaaaattt
     4561 gttgatcaag tgaaaccaca aaatattgac tgggaagctg ttggaaagca tttggatgaa
     4621 tgggtagaaa aagctgaatt agaatacgta aaataagaaa taagagttat tttttatgtc
     4681 caagtctctc tattatttct aaatttaagg aagaaataaa gagaggcttt tggttttagt
     4741 aatagaggga gttagtgaga tgattaaatt tgataatatt caaattaaat atggtgattt
     4801 tgttgcaatt gataatctga atttagatat acatgaaggg gaattcttta catttcttgg
     4861 gccttcagga tgtggtaaat caactacttt gagagcattg gtaggttttt tagatccatc
     4921 atcaggaagt attgaagtta atggaacaga tgtcactcat ttggaacctg aaaagcgtgg
     4981 aattggtatt gtatttcaat cttatgcgct atttccaact atgactgttt ttgataatat
     5041 tgcatttggt ttaaaagtta agaaggtagc tccagatgtt attaaagcta aagtatcagc
     5101 agtggcagca aaaattaaga tctctgatca acagttacag cgtaatgtat cagaattatc
     5161 tgggggtcaa caacaaaggg tagcattggc tcgtgctctg gttcttgaac ctaaaattct
     5221 ttgtctagat gaaccattgt caaaccttga cgcaaaatta cgtgtagatt tgagaaaaga
     5281 gttgaaaaga cttcaaaaag agttaggtat tactacttta tatgttactc atgatcaaga
     5341 ggaagccttg actttatctg atagaattgc agtctttaac aatggataca tcgaacaggt
     5401 cggtacacca gtagagattt atcataattc tcaaactgaa tttgtatgtg atttcattgg
     5461 agatattaat gttttgaccg atgaaacagt ccacgaagta ttattgaaaa atacaagcgt
     5521 tttcttagag gataaaaaag gatacattcg attagagaaa gttcgattca atcgtgaaac
     5581 tgaacaagat tttattctaa aagggacaat tattgatgtt gagttttctg gagttacaat
     5641 tcactataca ataaaagttt ctgaaagtca gattcttaat gtaacaagta ttgatagtca
     5701 ggctgctatt agatctgtcg gagaaagtgt ggaattattt atcacaccat cagacgttct
     5761 gcaattttaa ggaggtgcag tatgcgtcat aaattaaatt taaaagattg gcttattcgt
     5821 ttagggttaa tctggttctt agtaacattt attatttatc caaactttga tctagtagtg
     5881 aatgtatttg taaaaggagg agaattttcc cttgatgctg tacatcgtgt tctaaaatct
     5941 cagagggcac ttcagagtat tatgaacagt tttaagttag cattttcact cattattaca
     6001 gttaatgtcg taggtattct ttgtgttcta tttacagagt actttgatat taaaggtgct
     6061 aaaattttaa aattaggtta tatgacctct ttaatttatg gaggagtggt tttagcgact
     6121 ggatataaat ttgtctatgg tccttatgga ttgattacaa aatttttaca aaatgttatc
     6181 ccttctttag actctaactg gtttattggg tatggtgcag tcttattcat tatgacattt
     6241 tcaggaactg ctaatcatac attgttttta acaaatacaa ttcgaagcgt tgactatcac
     6301 actattgagg ctgctcgaaa tatgggagca aaaccattta ctgttttccg aaaagtagtg
     6361 ttaccaacct taattccaac tctatttgca cttactatta tggtttttct tagtggttta
     6421 tctgcagtag cagcacccat gattgttggt ggtaaagaat ttcaaactat aaatccaatg
     6481 attattacat ttgcagggat ggggaattct cgtgatttag ctgccttact tgcaattatt
     6541 ttaggtattg caactacaat tttgcttact atcatgaata agatagaaaa aggtggaaat
     6601 tatatttcta tctctaagac taaagcgcct cttaaaaaac aaaaaattgc gtctaagcct
     6661 tggaatatca ttgctcacat tgtagcatat ggattgttca cagttttcat gcttccacta
     6721 atttttatag tattatactc atttacagat ccagttgcaa ttcaaacagg taacttaaca
     6781 ttatcaaact ttactttaga aaattatcgc ttattcttta gtaatagtgc ggcattctct
     6841 ccattcttgg tcagctttat ttattctatt attgctgcga caacagcaac aattctcgca
     6901 gttgtatttg ctcgtgttgt cagaaaacat aaatctcgtt ttgatttctt atttgaatat
     6961 ggtgctctac ttccttggtt actaccaagt acacttttag cagtaagttt attatttact
     7021 tttaatcagc cacaatttct tgtcttgaat cagattttgg taggtagttt ggtaattcta
     7081 cttattgcat atatagttgt aaaaatccca ttttcttata gaatggtacg tgctatttta
     7141 tttagtgttg atgatgagat ggaagatgca gcaagaagta tgggtgcttc acctttttat
     7201 actatgatga aggttatcat tccatttatt ttaccagttg ttctctctgt tattgcttta
     7261 aactttaact ctttattaac tgacttcgac ttatctgtat tcctttacca tcccctagct
     7321 caaccattag gtattacgat tcgatctgca ggtgatgaaa cagcaacatc taatgcacaa
     7381 gctctggtat ttgtttatac aattgttctg atgattattt ctggaacggt attatacttc
     7441 acacaaagac cggggcgtaa agtaaggaaa taatcatgac agccactagt cttgggttat
     7501 caaatattga aatagttgtc aggattgttt tatcagtagt cattggtagt ataattggtt
     7561 tagagagagg gagcaaatcc cagcctgcag gcatccgaac ttatagtatt gtttgtctag
     7621 ctgcatgttt gattatgatg acgaatgaat acgtatctta taaatttggg acaggagatc
     7681 ctacacgatt aggagctcaa gttatatcag gtgtgggttt tctaggcgct ggaacgattc
     7741 ttattacaga taaaaagaaa attacaggtc tgacaactgc agcaggcatt tgggcttcgg
     7801 caggaattgg attagctatt ggagtaggtt tttatgaggg agctctttta gtagccattt
     7861 ctgtttgggg tgtgatatcc atgttccaac cactaaaaaa atatctgcaa aatcgttcta
     7921 aaatgattga attgtatata gtggttaaat ctacagaagc ctataatcgt gtattggttt
     7981 attgtgcaga aaatggtatc agaatgaccg attcaagaac agcgtttgga gatgttaatt
     8041 ctgacagaat tgaatacttt gatgttccgg acaaaaaaat agcgtcattt attactctgg
     8101 aattatcagg tagatttgaa catcttcgac tgatggaaga aattgctaat attgtaggtg
     8161 ttatttatgt cgaagaaatt agctgaaatt tagtaaacaa agggacagat gtttttgtcc
     8221 ttttgttttt tagatgtttt aatcatctaa tttttgagta gatttttgct cagaggtttt
     8281 tatgaaagat tataaaatca attttgattt aggtaaaata gaatattttg ataataattg
     8341 tttgatacaa gtatacaaat ttatttcttt ttatgatatt tgtgaaatgg tgtttgcctt
     8401 tcatttacct cctgatgagt tgataacaaa tgttatcttt aaagaaaaaa ttaactctat
     8461 gttaaaatgt tacattgaca ggctattata tgtttttata aa
//
LOCUS       DAVHEX010000076         8110 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVHEX010000076 DAVHEX010000000
VERSION     DAVHEX010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8110
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.077"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..144
                     /locus_tag="VVL45_001926"
     CDS             <1..144
                     /locus_tag="VVL45_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188054.1"
                     /translation="GLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFDMAKTQI
                     ETD"
     gene            236..1915
                     /locus_tag="VVL45_001927"
     CDS             236..1915
                     /locus_tag="VVL45_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEU4188055.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIV
                     GEYEDAGKSDKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLHIM
                     QDYGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENISIQTMEGRIQKAGEGKWNG
                     GFAPYGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAI
                     VSDEVWQAAQVKLKSQAKKYEHVNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKD
                     GTKYKDFYYYGCNHRQMIRGNKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQEK
                     INMKVDTSEIEKEIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYR
                     MYDKLDELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLISAL
                     ISEIQVYEEKQSNGQWLKSITFKLPIIEENLNIGLDNDEQVECVSLLEKRS"
     gene            2183..2749
                     /locus_tag="VVL45_001928"
     CDS             2183..2749
                     /locus_tag="VVL45_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188056.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            3071..3259
                     /locus_tag="VVL45_001929"
     CDS             3071..3259
                     /locus_tag="VVL45_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEU4188057.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            3321..4079
                     /locus_tag="VVL45_001930"
     CDS             3321..4079
                     /locus_tag="VVL45_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU4188058.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEVILYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVHDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVIF"
     gene            4137..4991
                     /locus_tag="VVL45_001931"
     CDS             4137..4991
                     /locus_tag="VVL45_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEU4188059.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGIWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCSIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            5103..5987
                     /locus_tag="VVL45_001932"
     CDS             5103..5987
                     /locus_tag="VVL45_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEU4188060.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            6150..6512
                     /locus_tag="VVL45_001933"
     CDS             6150..6512
                     /locus_tag="VVL45_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
                     /protein_id="HEU4188061.1"
                     /translation="MELSAIYHRPESEYDYLYKDKKLHIRIRTKKGDIESINLHYGDP
                     FIFMEEFYQDTKEMVKITSGTLFDHWQVEVSVDFARIQYLFELRDTEGQNILYGDKGC
                     VENSLENLHAIGNGFKLP"
     gene            6493..7889
                     /locus_tag="VVL45_001934"
                     /pseudo
     CDS             6493..7889
                     /locus_tag="VVL45_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
     gene            7975..>8110
                     /locus_tag="VVL45_001935"
     CDS             7975..>8110
                     /locus_tag="VVL45_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000995815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188062.1"
                     /translation="MNCRGHETRQRIVRDFEVQPKAHIKLLANQQKHSDAGATIEDEY
                     Y"
BASE COUNT         2825 a         1160 c         1725 g         2400 t
ORIGIN      
        1 ggattggctg atttaatagc aaatctgatt gcaaaatatg ctggtgcttt agatttagat
       61 aatctacccg atccaacgcc agcaaaaaat caagagatga aaaatagttt tgacatggca
      121 aaaacacaaa ttgagacgga ctaataaaag tgttataata tacttgaaat gatagtccaa
      181 acttaaatta aaaaaattat atatgatata gaaaaattat attgaggtta atactatgtc
      241 aaaagaaaaa ataaaagtat acctctatac acgagtatct acgtcaatac agatagaggg
      301 gtattcgtta gaggcacaaa aatcacgaat gaaagctttt gctatttaca acgattatga
      361 aattgttgga gagtatgaag atgccgggaa gtctgacaag tctattgaag gtagaataca
      421 gttcaatcga atgatggaag atataaaatc tggaaaagat ggagtatctt ttgttcttgt
      481 gtttaagtta tcaagatttg caagaaatgc cgctgatgtt ctttctacac ttcatataat
      541 gcaggattat ggagttaatc ttatctgcgt tgaagatggc attgattcat ctaaagatgc
      601 tgggaaacta atgatttctg ttttatcagc tgtggctgaa atagaaagag aaaacatcag
      661 tattcaaaca atggaaggtc gcattcaaaa agcaggggaa ggaaaatgga atggtggctt
      721 tgctccgtat ggatataaac ttgaagacgg caagctgttt ataaatgagg aagaggcagt
      781 tgcaataaga acgattttcg accagtatgt aaatactacg ataggagcca atgggatatc
      841 taaatactta gagaatcatg gaattagaaa aatcccaaga cagaatggta agaatccttt
      901 gtttgatgca ggtcttataa gaaagatatt aaagaatcct gtatataatg ggaaaatagc
      961 atttggaaga agaactttag aaaaagttca tggtacaaga aatgaatata agcaggttga
     1021 acaagatgaa tatctaatat ctgaagggat acatgaagct atagtttccg atgaagtttg
     1081 gcaagctgct caggttaagc taaaatctca agcaaagaaa tatgagcatg tgaataaagg
     1141 aaaagataca cgcacacact tgctttctgg aattgtaaaa tgcccgatat gtggagtggg
     1201 aatgtttggg aacaagtgta tcaagaaaaa gaaagatggc acaaagtata aagattttta
     1261 ttactatggt tgtaaccata ggcagatgat aagaggtaat aagtgtacat tcagtaagca
     1321 aattagagaa gaattgttag atgatgctgt tgcagaggtg attgtcaaga tagtaagtaa
     1381 tccgaaattt gcttctatga tgcaagaaaa aatcaacatg aaggtggata cctctgaaat
     1441 agaaaaagaa atagataatt accaaaagga attgagaaag agtcattcta caaagtttaa
     1501 gctaattgag gaaatagata atttagatgt tgaagataag cattataaga gaaggaaaca
     1561 ggatttagat gatagactat atcgtatgta tgacaaacta gatgaattag aatcatcact
     1621 aattgatgcg aaagcaaaga aacagactat tgaagctgaa aagcttacag gagataacat
     1681 atataaggtt ctgatctatt ttgataagct ctataaagtc atgaatgatg tagagcgtag
     1741 gcagttaatt tcagctttga tttctgaaat tcaagtttat gaagaaaaac aatctaacgg
     1801 acaatggcta aaatccatta cttttaagct accaattata gaagaaaatc tcaacatagg
     1861 tttggacaat gatgaacaag ttgaatgtgt aagtttatta gagaaacgta gttaatcctc
     1921 aaaaggttcc ccgaaccttt tgagtcccac tgacgcatca cgctgaggcg gtatcactgc
     1981 ttgtacgagt tgaagcatca gcaaagtaaa agtagatgtt cagccaatgc gacgaggtga
     2041 gaccgaaggt cgataaggtg tgttctgggt ataagactcg taaagtaagt aggaaatatg
     2101 atgagatgat acagagagcc tgaattggtc aggtgacgtg cttatttaca ttagcctgat
     2161 aatgaaaatg gagagattat cgatggttat attggaattg taccaaaatg attattctaa
     2221 agatttagta gcatttgatt ccatagaaga tgggaaagcc tttgtagctc aaatcccggg
     2281 atacactctg gaaacagaag atggttttga agtggagtat tttaatccta aaaacatacc
     2341 ggattatatg gaaattattt ttaacggaaa tattgttcct ttatctaaat ttatgttcga
     2401 tcctgaggaa aatgtaaaca ttatttggaa agagatttca aatttatccc tcaaaaatga
     2461 tagagtgatc gaaggatatt caaaaattga tgcatatgtc gttaacaacc atgaagttaa
     2521 agcctatgtc gaaacaagag aaacgaacta tcgtaaagca aaagacttct tagaaagccg
     2581 cggatatgaa atcgacagaa gctttttcgg aagtgaagac ggtgaagcga ttctttacag
     2641 aaaaaaaggg atcgaagttt ggcatttctt gtgtcactta gacccaatgt ttgtagagat
     2701 tgaagatgta gaaggatatg ttaaagaaga agttggagag attcaatagt caaccaaagt
     2761 tccttgtgag tatatacctt tgctagtaaa agagtcaaaa cgttataacg aaggggggga
     2821 agaaaaccct tgagtgtaag tctttttctt gtttagaggg aaagtattag agtagccgct
     2881 cataatttat actagatttg ataactgatt tgagacagat tttatttcta taaatattgt
     2941 gaaaattttt taataccaaa gatttaaagt tactgtgtta aaaatcctct ccacttttaa
     3001 actaatgtat tataaaagcc agttgacact agtgttacac ctgtgataga attaacttga
     3061 ggtgatgaaa atggcaaccg ataaaaatag aataatgatt agcttggatg ataaaaatct
     3121 agagaaactt gaaaatttag tagaagatgc tagagataga aggggaatgc gactaacaaa
     3181 gtcccaggtt attgaattac tcctaaatac cgttgattat ttcgatgata ttatgggggc
     3241 tatttactca aaaaaataag agcaaaattg ctattatttt ttacaaattg gtgtaacact
     3301 agtgatatag gagtgatgaa atggttgttt atattagaca aagtaaatta ccaagtgagg
     3361 tttcaattaa taaatacaat gctcaagtag gtgcctactt acaaggagaa gaagttattt
     3421 tatatcaatc tttttctgaa ataaaagagt taacaagtga agatatagtt gtagattata
     3481 taatggagac tagagcatta ctaaaaatga tgggcttaaa cgttccggtt catgactatc
     3541 ctattgagct taaagagttt tatggtcgaa agatttatgc tggtatttta ggagagattg
     3601 tgaatatacc tgataattgg ggaaaattta ttaagcctaa agctggttca aaagtcttta
     3661 ctggaagagt tgttaatgga acccatgatt taataggtat tggtctacct ttcgactatc
     3721 ctatatggat tagtgaggtt gtagaattca tagctgaatg gcgttgtttt gtgttagatg
     3781 gtcgcgtatt agatgttcga ccctatacag gtgattatca tgcacaattt gatgcaagtg
     3841 taattgatga agcgatatca tgttggaaag atgcgccaat agcttatgga ctagatatcg
     3901 gtgttactcg cgatggcaga acacttgttg ttgaagtaaa tgatggttat gcattgggaa
     3961 attatggcct atctccttta aaatcaatca attttcatag agctaggtgg aaagaaatgg
     4021 taaaacccta ttttgaaaaa aatgaaattt ttaaaatcca acaggatgtt attttctaaa
     4081 ataaatatgg tagataacca atataaaaag catcaaaatg aacaaggagg aatctgatgc
     4141 ttggagcaat tattggagat attgtcggtt ctgtttacga atggaacaat atcaaaacaa
     4201 aggactttcc tttatttcgg aaggactgct tttttacaga tgacacggtt atgacctgtg
     4261 ctgttgcaga agcaattatg aatggtggac aaaaagatga cttcattgac gcgatgaaga
     4321 aatatggcag aatgtatccg aatgctgatt acggtgctcg gtttaatgca tggctaaaca
     4381 gcgataaccg tgagccttat aatagctttg ggaatggatc agctatgcgt atttctccat
     4441 gtgcttggat catggactgt ggtttttatg cgaaaactgg tatatggcca tcatctagag
     4501 gacttacgag tctttctgca gaggtaactc ataatcatcc agaaggtgtc aaaggcgcta
     4561 tggctacagc tgatgctatc tttctatgtc gtttttactt tggtggttat tgtagagagt
     4621 acgagcaatc aattaacgac aatcctacag agtgtaaaag acgaattaag gattatatag
     4681 aaaaagaata cgattacaat ctatctcaaa ctttagatga aatccgtcct aactatcgtt
     4741 ttaacgaaac atgtcaggaa actgtccctc aagccattat cgcctttctt gaaagtagag
     4801 acttcgaaga tgcgataaga aatgccatct cacttggtgg cgacagtgat actctcgcag
     4861 caatcacttg tagcatagca gaggcagctt acggtattcc tgattggatc aaggataagg
     4921 cctattctta cttggatgaa cccttaaagg atgtagttag gcgatgggaa aatagaataa
     4981 aagcgtatta atctccagta cgtcagattt ctcttttctg tattttcatt gtataatagg
     5041 attacattta cttgcatacg acgaaatgta aacatttctg tttaaaatta aggataaaat
     5101 caatgaaaaa agcaatggta attatcaacc ctacttctgg tggcgagaag gctttggatt
     5161 acaaagaaaa gctggagaat aaagcaaaag aatactttga atatgttgaa accaaaatta
     5221 ccgaaaaagc gctggatgca acacattttg ctgaagaagc ttctcgtgag cagtatgatg
     5281 cagtggttgt gtttggtgga gatggaactg tcaatgaagt catttcaggt attgatgaga
     5341 gagactacat tcctaagtta gggattatcc caggcggtac gggtaacctc attacaaaac
     5401 ttttggaaat caatcaagac atcgatggcg caattgacga actggatttt gatttaacca
     5461 ataagattga tatcggtaaa gcaaatgaca actattttgg ttatatcttt agtatcggtt
     5521 ctctgcctga ggcgattcac aatgttgaaa tagaggacaa aacaaaattc ggtattttaa
     5581 cctatgctgt aaataccatg aagtctgtca tgacagatca ggtctttaac attaaggttg
     5641 agacagaaaa tggaaattat gttggtgaag ctagccatgt tttggtcctt ttgacaaatt
     5701 acttcgctga taagaaaatc tttgaagaaa acaaggacgg ctatgccaac attttgattc
     5761 tgaaagatgc ctctatattc tccaaattat ccgtcattcc tgatttatta aaaggggatg
     5821 ttgtcgcaaa tgataatatc gagtatatca aagcgcgtaa tattaaaatc tcttcagata
     5881 gtgaattgga gtcagatgtt gacggagata aatcagataa cctacctgta gaaatcaaag
     5941 tcctagctca gcgagtagaa gtattttcaa aaccgaaaga ggattagtat atagagaaag
     6001 ccttttttaa ggctttttgt atactttaaa agatagttcc tttaacaacg gacattcctt
     6061 gcaaatagtt tacaaaaata gtatactgga ttcattgagt ttgaaaacgt ttgcgtaaaa
     6121 tttgaatgaa tactttagga gacaaattga tggaattgag tgctatttac cataggcctg
     6181 agtcggagta tgactatctt tataaggata agaaactcca tattcgaatt cgaactaaga
     6241 aaggggacat tgaaagcatc aacttgcact atggggaccc ttttatcttt atggaggagt
     6301 tttatcagga tacaaaagaa atggtcaaga taacttctgg taccttattt gaccattggc
     6361 aggttgaagt gtcagttgac tttgcacgta tccagtatct ctttgagctc agagatacag
     6421 aaggtcaaaa tattttgtat ggcgataaag ggtgtgtgga aaattctcta gaaaatcttc
     6481 atgcaattgg gaatggattt aagttgcctt agcttcatga gattgatgcc tgcaaggttc
     6541 ctgactgggt ttcaaatacg gtatggtatc agatatttcc tgaaagattt gccaatggca
     6601 atgctctatt aaacccagaa gggactttag actgggattc atctgtcaca cctaagagcg
     6661 atgatttctt tggtggtgat ttacagggga ttattgatca tatgaattac ttgcaagact
     6721 tgggtattac tggactatat ctttgtccca tctttgaatc tacaagcaat cacaagtaca
     6781 atacgacaga ttactttgaa attgaccgtc attttggaga caaggagacc tttcgggaac
     6841 tggtggatca agcgcatcat cgtggcatga aagtcatgct ggatgcggta tttaatcata
     6901 ttggttcgca atctcttcaa tggaaaaatg tcgtcaaaaa tggtgaacag tctgcttata
     6961 aggattggtt ccatattcaa caattcccag tgacaactga aaagctagtt aataagagag
     7021 acttacccta tcatgttttt ggtttcgagg actatatgcc taagctaaat acagccaatc
     7081 cagaggtcaa gaattatctt ttaaaggttg cgacttattg gattgaagag tttaatatcg
     7141 atgcttggcg tttggatgtg gctaatgaga ttgaccatca gttctggaag gattttcgta
     7201 aggcagtttt agctaaaaat cctgatcttt atatcctagg agaagtctgg catacatctc
     7261 agccttggct aaatggagat gagttccatg ccgtcatgaa ttatccttta tctgatagta
     7321 tcaaggacta tttcttacga ggaattaaga agacagacca gttcatcgat gaaatcaatg
     7381 gagagtctat gtattacaag cagcagattt cagaggtcat gtttaatctc ttggattcac
     7441 atgatacaga gcgaatcctg tggacggcca atgaagatgt tcaactggtt aaatcagcct
     7501 tagcctttct ctttttacaa aaaggaacac cgtgcattta ttacggaacc gagctagcct
     7561 tgactggagg accagatcca gattgtcgtc gttgtatgcc ttgggaacgt gtatcaagtg
     7621 acaatgatat gctgaacttt atgaagaggc tgattaaaat tcggaaatac gcgtcagtaa
     7681 tcatttcgca tggcaagtat agccttcaag aaatcaactc tgatctagta gctctggaat
     7741 ggaaatacga aggacggatc ctcaaagcaa tattcaacca atcaacagaa gattatcttt
     7801 tagagaaaga agcagtagca ctagcaagca attgccaaga attggataat cagcttgtca
     7861 tctctccaga tggatttatg attttctaaa aactagttga tgaagattat ggtacatttc
     7921 ataccttata tagtataata aggctagtta ctaaacttgt aaaggagaac ttaaatgaat
     7981 tgtagaggac atgaaacaag acaaagaatt gttagagatt ttgaagttca gcctaaagca
     8041 catattaagc tgttagcaaa tcaacaaaaa catagtgatg caggagcaac tattgaagat
     8101 gaatattatg
//
LOCUS       DAVHEX010000077         8084 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVHEX010000077 DAVHEX010000000
VERSION     DAVHEX010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8084
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.078"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..227
                     /locus_tag="VVL45_001936"
     CDS             <1..227
                     /locus_tag="VVL45_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU4188063.1"
                     /translation="TEKMAVTSPVRAQSRVPFSEEKDIAFYDSKDYQIVELLPGNMLV
                     TFEEDLHQPKIHCNDETVKKLVIKVLNEEK"
     gene            229..2451
                     /locus_tag="VVL45_001937"
     CDS             229..2451
                     /locus_tag="VVL45_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEU4188064.1"
                     /translation="MKKNIKQYVTLGTVVVLSAFVANSVAAQETETSEVSTPKLVQPV
                     APTTPISEVQPTSDNSSEVTVQPRTVETTVKDPSSTAEETPVLEKNNVTLTGGGENVT
                     KELKDKFTSGDFTVVIKYNQSSEKGLQALFGISNSKPGQQNSYVDVFLRDNGELGMEA
                     RDTSSNKNNLVSRPASVWGKYKQEAVTNTVAVVADSVKKTYSLYANGTKVVEKKVDNF
                     LNIKDIKGIDYYMLGGVKRAGKTAFGFNGTLENIKFFNSALDEETVKKMTTNAVTGHL
                     IYTANDTTGSNYFRIPVLYTFSNGRVFSSIDARYGGTHDFLNKINIATSYSDDNGKTW
                     TKPKLTLAFDDFAPVPLEWPREVGGRDLQISGGATYIDSVIVEKKNKQVLMFADVMPA
                     GVSFREATRKDSGYKQIDGNYYLKLRKQGDTDYNYTIRENGTVYDDRTNRPTEFSVDK
                     NFGIKQNGNYLTVEQYSVSFENNKKTEYRNGTKVHMNIFYKDALFKVVPTNYIAYISS
                     NDHGESWSAPTLLPPIMGLNRNAPYLGPGRGIIESSTGRILIPSYTGKESAFIYSDDN
                     GASWKVKVVPLPSSWSAEAQFVELSPGVIQAYMRTNNGKIAYLTSKDAGTTWSAPEYL
                     KFVSNPSYGTQLSIINYSQLIDGKKAVILSTPNSTNGRKHGQIWIGLINDDNTIDWRY
                     HHDVDYSNYGYSYSTLTELPNHEIGLMFEKFDSWSRNELHMKNVVPYITFKIEDLKKN
                     "
     gene            2491..3600
                     /locus_tag="VVL45_001938"
     CDS             2491..3600
                     /locus_tag="VVL45_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU4188065.1"
                     /translation="MVNYGIVGAGYFGADLARSMNKIEDAKVVAVFDPNHGEEVAQEL
                     GSDVCASLDELVAREDIDCVIVASPSYLHREPVVKAAQHGKHVFCEKPIALSYEDCKA
                     MVDACKENNVIFMAGHIMNFFNGVHHAKELITQGKIGKVLYCHAARTGWEEQQPTVSW
                     KKLRSQSGGHLYHHIHELDCIQFIMGGLPEKATMVGGNVYHKGENFGDEDDMLIVNLE
                     YSDDRYAVLEYGNAFRWGEHYVLIQGTEGAIKLDLFNTGGTLRVKGEGESHFLVHETQ
                     EEDDDRTAIYTGRGMDGAIAYGKPGVRCPLWLQTCIDKEMEYLHDIIKGGEITEEFEK
                     LLNGVAALESIATADACTLSVKEDRKVSLSEITNA"
     gene            3757..4647
                     /locus_tag="VVL45_001939"
     CDS             3757..4647
                     /locus_tag="VVL45_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU4188066.1"
                     /translation="MKIIGIDIGGTTIKADLYDEFGTSLNHFKEIETIIDYDLGTNQI
                     LNQVCDLIGEYTLNHSIDGVGISTAGVVNANTGEIIYAGYTIPGYIGVNFTAEIEKRF
                     GLYTFVENDVNCAALGELWKGQAKDKKNVVMVTIGTGIGGSIIVNGQIVNGFNYTAGE
                     VGYIPVGNSDWQSKASTTALIHLYQKKSLKTNQTGRTFFTDLRSGDKVAEETFEIFVE
                     NLTKGLLTISYLLNPEILILGGGILDSKDILLPEIQSSLAKNAMDNRFLPKNLVAATL
                     GNEAGRIGAVKNFLDRISNK"
     gene            4672..4995
                     /locus_tag="VVL45_001940"
     CDS             4672..4995
                     /locus_tag="VVL45_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188067.1"
                     /translation="MTNSVFSTMQDIENVATDIIKSYDNEIYTYKAVSQEELEKLEKS
                     YDEKSHEELVSIESNLEMKQQNLIDEVNKTIKENDANIQYISSSRRGEFVEKIIGRVV
                     EKYGH"
     gene            4985..6976
                     /locus_tag="VVL45_001941"
     CDS             4985..6976
                     /locus_tag="VVL45_001941"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEU4188068.1"
                     /translation="MAISQMKRISLLFSKSSLDDVLKTIQELESVQFRDLKVQDNWSE
                     ALEKDEVVFPTIQIFHTSNSNHGVIEGNDALTYLMNQQQHLEATVEKLQEYLPKENTF
                     KLLQQPPITTSYEELEKFGKANVAEGVLKKVNHQINRVHELERHIQSNNEEIERLIKW
                     EKLEIVPANLEQFSFCKGKVGTIPRTEDNRLYNSLLENNIEVQEIFSNDREYGVVVFY
                     QSSYSIDFDEYLFEPFDYSRKELPKQRVVDLDQENMQLITEKENIIASLQDSKKYLID
                     LQWQIDYILSIYARQISKNNFLCTPHLVALEGWIEETRILYFIKVMDEHFGHSIYIYE
                     SETLTDNQDEIPIKLTNHSLIEPFELLTEMYALPKYYEKDPTPVLAPFYFTFFGMMVA
                     DLGYGLLLFLGTMLALKIFHLPSATKRFLKFFNILGVAVAIWGGIYGSFFGYELPFHL
                     ISTTSDVMTILVVSVVFGFITVFAGLLASGLQKVRMKKYAEAYNSGFAWCVILLGLLF
                     IAVGMLMPDMRPLFVLGKWVSIFNAVGILIVSIIQTKSLSGIGAGLFNLYNISSYIGD
                     LVSFTRLMALGLSGASIASAFNLIVGLFPGILAKLTIGLVLFILLHAINIFLSLLSGY
                     VHGARLIFVEFFGKFYEGGGKPFQPLKASEKYIKVITKN"
     gene            6992..7468
                     /locus_tag="VVL45_001942"
     CDS             6992..7468
                     /locus_tag="VVL45_001942"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEU4188069.1"
                     /translation="MEHLATYFSTYGGAFFAALGIVLAVGLSGMGSAYGVGKAGQSAA
                     ALLKEQPEKFASALILQLLPGTQGLYGFVIGILIWLQLTPELPLEKGVAYFFVALPIA
                     IVGYFSAKHQGNVAVAGMQILAKRPKEFMKGAILAAMVETYAILAFVVSFILTLRV"
     gene            7502..8083
                     /locus_tag="VVL45_001943"
     CDS             7502..8083
                     /locus_tag="VVL45_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188070.1"
                     /translation="MSNLENLRESVIEQAHEKGRMKLLDSKKKIDDEFEMQKSLIIKK
                     KEAEHERKLKELQQKYQIIFQQLKNKERQSTLVSKQKILKELFQSALLEMESWSADKE
                     MEFIYRILERYSQQEVIVTFGERTLAKFNLEQLEKLKFSFPNYLFSEQPISNESGLLI
                     SIGKIDDNYLYKTLIGSISKEESSSIANQIFIN"
BASE COUNT         2798 a         1131 c         1612 g         2543 t
ORIGIN      
        1 atactgaaaa aatggcagta acttccccag ttcgtgccca gtcacgtgta ccttttagcg
       61 aagagaagga tattgctttt tacgatagca aagactatca aattgttgaa ttgcttccag
      121 ggaatatgtt ggtgactttt gaagaagatc ttcaccaacc aaagattcat tgcaatgatg
      181 aaactgttaa aaaacttgtt atcaaagttt taaacgagga aaaataaaat gaaaaaaaat
      241 attaaacaat atgtaacctt aggtactgta gtggtattat cagcatttgt tgctaactca
      301 gttgcagctc aggagactga aacttctgaa gtatcaacac caaagttggt gcaacctgtt
      361 gcaccaacga ctccgatttc ggaagtacaa cctacatcgg ataactcttc ggaagttact
      421 gtacaacctc gaacagttga aactactgtt aaggatccat cttctacagc ggaagaaact
      481 cctgtcttag aaaaaaataa tgttacttta acagggggcg gagaaaatgt tactaaagag
      541 ttaaaggata aatttactag cggtgacttt actgtagtga ttaagtacaa tcagtcaagt
      601 gagaaaggct tacaagctct gtttggaata tctaattcca aacccggtca acaaaatagt
      661 tatgtagatg tgttccttag agacaatggt gagttgggga tggaagcgcg tgatacttct
      721 tccaataaaa ataacctagt atccagacct gcttcagttt ggggtaagta caaacaagag
      781 gctgtgacta acactgttgc agtagtagca gattcagtca aaaaaacata ttctttatac
      841 gcaaatggta caaaagtagt agaaaagaaa gtggataatt tcctaaacat caaggatatt
      901 aaaggtattg attactatat gcttggggga gtgaaacgtg caggaaaaac ggcgtttggt
      961 tttaacggaa cactagaaaa tatcaaattc tttaatagtg cattggatga agaaactgtt
     1021 aaaaagatga caacaaacgc tgttactgga catttaattt atacggctaa tgatacaaca
     1081 ggttctaact atttccgtat tccagttctg tatactttta gcaatggtcg ggtattttca
     1141 agcattgacg ctcgttacgg tggaactcat gatttcttga ataaaattaa tattgctaca
     1201 agttatagtg atgataatgg taagacatgg actaaaccaa aattaacatt ggcattcgat
     1261 gattttgcgc cagtaccatt agaatggcct cgtgaagttg gtggacgtga cttacaaatc
     1321 agcggtggtg caacctatat tgactctgtt attgttgaaa aaaagaacaa acaagtactc
     1381 atgtttgctg atgtgatgcc tgctggagta agttttagag aagcaactag aaaagattca
     1441 ggttataaac aaattgatgg taattattac cttaaattaa ggaaacaagg tgatactgat
     1501 tacaattata ctattcgtga gaatggtact gtatacgacg atcgtaccaa cagaccaact
     1561 gaattttcag tagataaaaa tttcggtatt aaacaaaatg gtaattattt gacggtagag
     1621 caatattcgg tttcatttga aaataataaa aagactgaat atcgtaatgg gactaaagtc
     1681 catatgaata tcttctacaa agatgcattg tttaaagtag tgccaacgaa ctatattgca
     1741 tatatttcta gcaatgatca tggcgaatct tggtctgcac caactttatt acctcctata
     1801 atgggactta atcggaatgc gccatattta ggtcctggac gtggaatcat tgaaagctca
     1861 actggacgta ttcttattcc gtcttacact ggtaaagagt ctgcgttcat ttatagtgac
     1921 gataatggag catcttggaa agttaaagta gtgccacttc cttctagttg gtcagcagaa
     1981 gcacaatttg tagaattgag tccaggagta attcaagcat atatgcgtac aaataatggt
     2041 aaaattgcat atttaacaag taaagacgca ggtactactt ggagtgcacc ggaatatttg
     2101 aaatttgttt caaatccaag ttatggaaca caattatcaa tcatcaatta tagccaattg
     2161 attgatggta aaaaggctgt cattttaagt actccaaact ccacaaatgg tcgtaaacac
     2221 ggacaaattt ggattggtct aattaatgat gataatacaa ttgattggcg ttatcatcac
     2281 gacgttgatt atagtaacta tggatactca tattcaacat tgacagagtt accaaatcat
     2341 gaaattggat tgatgtttga aaaatttgat tcatggtctc gtaatgaact tcatatgaaa
     2401 aatgttgtac catatataac atttaagatt gaagatctga aaaagaatta aagctgaaat
     2461 ttgaaaatat ataaaaagag gataaaaatt atggtaaatt acggtattgt tggagctgga
     2521 tattttggag ctgatttagc tcgctcaatg aacaaaattg aagatgcaaa agtggttgcg
     2581 gtatttgacc caaatcatgg agaagaagtt gctcaagagt tgggatcaga tgtttgtgca
     2641 agtttagatg aacttgtagc acgtgaagat attgattgtg tgatcgtagc ttcacctagc
     2701 taccttcacc gtgaaccagt tgtgaaagct gctcaacatg gcaaacacgt attttgtgaa
     2761 aagccaattg cattgtctta tgaagattgt aaagccatgg ttgacgcatg taaagaaaat
     2821 aatgtcatct ttatggctgg tcacatcatg aacttcttta acggtgtaca ccatgctaaa
     2881 gaattgatta ctcaaggtaa aatcggtaaa gttctttatt gccatgctgc tcgtacaggt
     2941 tgggaagaac aacaaccaac tgtatcatgg aagaaacttc gttctcaatc tggaggacat
     3001 ttgtaccacc atattcatga attagattgc attcagttta tcatgggagg acttcctgaa
     3061 aaagcgacaa tggtaggagg caatgtatat cataaaggtg aaaactttgg tgatgaagat
     3121 gatatgctca ttgtaaactt agaatactct gatgatcgtt atgctgtttt ggaatatggt
     3181 aatgctttcc gttggggtga acactacgtc ttgattcaag gaactgaagg agctatcaaa
     3241 cttgacttgt tcaatactgg cggtactctt cgtgttaaag gtgaaggaga atcacacttc
     3301 ttagttcatg aaactcaaga ggaagatgat gatcgtacag ctatctatac cggtcgtggt
     3361 atggatggag caattgcgta cggtaaacca ggagtacgtt gcccattatg gttgcaaaca
     3421 tgtattgata aagaaatgga atatctacat gacatcatta aaggtggaga aattacagaa
     3481 gaatttgaaa aacttctcaa tggtgtagct gctttagaat caatcgctac cgctgatgca
     3541 tgtactttat cagttaaaga agatcgaaaa gtaagtcttt cagaaatcac aaatgcttaa
     3601 cttttgtaaa acagaatagt aaattcttgt cattatataa tttctaaagt tctgtgatac
     3661 aactcattga ataaagaaat agagatggga ctgggataat gcccagtccc attttttatc
     3721 aaaaagtaat gagatcaaaa atgtgggagt gttgaaatga agattatagg tatcgatatt
     3781 ggcggaacaa caattaaggc agatttatac gatgagtttg gaacgagttt gaatcatttc
     3841 aaagagatag aaacaattat tgactatgat ttgggaacga atcagatatt aaatcaggtc
     3901 tgtgatttaa ttggtgagta tactttaaat cattcaattg atggtgttgg gatttccact
     3961 gctggagttg ttaatgctaa tactggagaa atcatctatg caggctatac aataccaggg
     4021 tatatcggag taaactttac tgccgaaata gaaaaacgtt ttgggttgta tacttttgtt
     4081 gaaaatgatg ttaattgtgc tgcattaggt gaattgtgga agggacaagc caaagataag
     4141 aaaaatgtag taatggttac tattggaaca ggtataggag gcagtattat tgtcaacgga
     4201 caaattgtta acggatttaa ctatactgct ggtgaagtag gttatattcc tgtaggtaat
     4261 tcggattggc aaagtaaagc ctcaacaacc gcattgattc atttatatca aaaaaagagc
     4321 ttgaaaacta atcaaactgg acgtactttc ttcactgatt taagatctgg agataaagtt
     4381 gctgaagaaa cttttgaaat ttttgtagaa aatctaacaa aaggtttatt aacgatttct
     4441 tatctactta atccagaaat tctcatatta ggaggtggga ttctggatag taaggatatt
     4501 ttgttacctg aaattcaaag ttctttagct aaaaatgcaa tggataatag gtttttacct
     4561 aaaaatcttg tggcagctac attaggaaat gaagctggtc gtataggagc tgtaaaaaat
     4621 ttcttagata gaatttctaa taaatagtat gtaagataag gaggtgtcac aatgactaac
     4681 tctgtatttt cgacaatgca agatattgag aatgttgcaa ccgatattat aaaatcatat
     4741 gataatgaga tttatactta taaagctgtt tcccaagaag aattggaaaa actagaaaaa
     4801 agttatgatg aaaaaagtca cgaagaatta gtttcaatag aaagcaattt agaaatgaaa
     4861 caacagaacc ttattgatga ggttaataaa acaatcaagg aaaatgatgc aaatattcag
     4921 tatatttcat caagtaggag aggagaattt gtagaaaaaa ttattggtag ggtggtagaa
     4981 aaatatggcc attagtcaga tgaaaagaat ctctctacta ttttctaaaa gtagtcttga
     5041 tgatgtttta aaaactattc aagaactaga gtcagtgcag ttccgtgatt taaaggttca
     5101 ggataactgg tcagaagctc tagaaaaaga tgaagttgta tttccaacta ttcaaatttt
     5161 tcatacttct aattccaatc atggggttat tgagggaaat gatgccttga cttatttgat
     5221 gaatcaacaa caacatttag aagcaactgt agagaaatta caagaatacc taccgaaaga
     5281 aaacacgttt aaattattgc agcaacctcc gataactacc tcttatgaag aattagagaa
     5341 atttggtaaa gctaatgttg ctgagggtgt tcttaaaaaa gtgaatcatc aaattaacag
     5401 agttcatgaa ttagaaagac acattcaaag taataatgag gaaatagagc gattaataaa
     5461 gtgggaaaaa ttagaaattg ttcctgcgaa tttagaacaa ttttctttct gtaaaggaaa
     5521 agtcggaaca attccaagga ctgaagataa tcgcttatac aatagtcttt tagaaaacaa
     5581 tattgaagtt caagaaatat tttctaatga tagagagtac ggtgttgttg ttttctatca
     5641 gtctagttac tctatagatt ttgatgaata cttatttgaa ccatttgatt attctagaaa
     5701 ggaattaccg aagcagcgag tagtagattt agatcaagaa aacatgcagt taataactga
     5761 aaaagagaat attatcgcat cgttgcaaga ttcaaagaaa tatttgatag atttacaatg
     5821 gcaaatagac tatattttat ctatctatgc tcgtcaaatc tctaagaata actttttgtg
     5881 cactccgcat ctagttgcat tagaaggatg gatagaagaa actcgtattt tatattttat
     5941 aaaagttatg gatgagcatt ttggacattc tatttatatt tatgaatcgg aaacattgac
     6001 ggataatcaa gatgaaatac ctatcaaatt aacgaatcat tctttaattg aaccatttga
     6061 attattgaca gaaatgtatg ctctgcccaa atattatgag aaagatccta cacctgtatt
     6121 agcaccattt tactttacat tttttggaat gatggttgct gatttaggct atggtttact
     6181 attgttttta ggaacaatgt tagcattaaa aatttttcat ctaccttcag caactaagag
     6241 atttttaaaa ttctttaata tattaggggt agccgttgca atttggggtg gaatctatgg
     6301 ctcatttttt ggatatgagt tgccatttca tctgatatct acaacctctg atgtcatgac
     6361 tatattagta gtgtcagttg tgtttgggtt tattacagta tttgcaggtt tgttagcttc
     6421 aggactacaa aaagtaagaa tgaagaaata tgcagaagca tataattcag gatttgcgtg
     6481 gtgtgttatt ctgcttggct tgttatttat tgctgttgga atgttgatgc ctgatatgag
     6541 accgttattt gtattaggga aatgggtatc tatttttaat gctgtgggga ttttgattgt
     6601 ttctattatt caaaccaaaa gcttgtcagg tattggagca ggattgttta atctatataa
     6661 catttcatct tatataggtg atttagttag tttcactcga ttgatggcat taggattatc
     6721 tggagcaagt atagcatcag ctttcaattt aattgttggt ttgtttccgg gaatattggc
     6781 taaactgaca attggattag tattattcat tcttttacat gcgatcaata tttttctatc
     6841 gttactatca ggatatgttc atggagcacg tctgatattt gttgaatttt ttggtaagtt
     6901 ttatgagggt ggaggaaaac catttcaacc tttgaaggct tctgagaaat atattaaggt
     6961 tattacaaag aattaatgga ggatatatat aatggaacat ttagcaactt atttttcaac
     7021 ctatggagga gctttcttcg ctgcattggg aattgtattg gcggttggat taagcggtat
     7081 ggggtctgct tatggagttg gtaaggctgg gcaatctgcc gcagctttac tgaaagaaca
     7141 gcctgaaaag tttgcctcag ctttgatatt gcaattattg cccggaacac aaggattata
     7201 tggttttgtt attggaattt taatttggtt gcaattaact ccagaacttc ctttagaaaa
     7261 aggcgttgct tatttctttg tagctcttcc aattgctatt gtaggatact tttcagctaa
     7321 gcatcaagga aatgtagcag tagcgggaat gcaaatcttg gctaaaagac caaaagaatt
     7381 catgaaggga gcaattttag ctgccatggt agaaacctat gcaattcttg cttttgtcgt
     7441 atcattcatt ttgacccttc gtgtataaga aataaatttg caattcaaag gaggtgtcta
     7501 aatgagcaat ttagaaaact tacgagagtc tgttattgaa caagctcatg aaaaagggcg
     7561 tatgaaatta ttggattcca aaaagaagat tgatgatgaa tttgaaatgc aaaagtcgct
     7621 cattataaag aaaaaagaag ctgaacatga acgaaagtta aaagaattgc aacagaaata
     7681 tcaaataatt tttcaacaat taaaaaataa ggaacgccaa tcaacgttag tatcaaaaca
     7741 gaaaatatta aaagaacttt ttcaatctgc tttactagaa atggaatctt ggagtgcaga
     7801 taaagaaatg gagttcatct atcgaattct ggaacgatat tcacaacaag aggtcatagt
     7861 aacctttggg gaacggactt tagctaaatt caatttggaa caattagaga aattgaaatt
     7921 ctcttttcca aattatttat ttagtgaaca acctatctca aatgaatcag gcttacttat
     7981 ttcaataggt aaaattgatg ataactattt gtataaaaca ttaattggat cgatttctaa
     8041 ggaagaaagt tcaagtatcg caaatcaaat ttttatcaat taag
//
LOCUS       DAVHEX010000078         7899 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVHEX010000078 DAVHEX010000000
VERSION     DAVHEX010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7899
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.079"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            6..851
                     /locus_tag="VVL45_001944"
     CDS             6..851
                     /locus_tag="VVL45_001944"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEU4188071.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            complement(998..2059)
                     /gene="dinB"
                     /locus_tag="VVL45_001945"
     CDS             complement(998..2059)
                     /gene="dinB"
                     /locus_tag="VVL45_001945"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEU4188072.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIRDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKSVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGVSYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILLAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSKKERGVRLLGITMTGF"
     gene            2407..4731
                     /gene="pflB"
                     /locus_tag="VVL45_001946"
     CDS             2407..4731
                     /gene="pflB"
                     /locus_tag="VVL45_001946"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEU4188073.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
     gene            complement(4869..6137)
                     /locus_tag="VVL45_001947"
     CDS             complement(4869..6137)
                     /locus_tag="VVL45_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU4188074.1"
                     /translation="MEHINHTTLLIGIKDKNITLNKAIQHDTHIEVFATLDYHPPKCK
                     HCKGKQIKYDFQKPSKIPFIEIGGFPSLIHLKKRRFQCKSCRKVTVAETTLVQKNCQI
                     SEMVRQKIAQLLLNREALTHIASKLAISTSTSTSTVYRKLKQFHFQEDYTTLPEILSW
                     DEFSYQKGKLAFIAQDFNTKKIMTILDNRRQTTIRNHFFKYSKEARKKVKVVTVDMSG
                     SYIPLIKKLFPNAKIVLDRFHIVQHMSRALNQTRINIMKQFDDKSLEYRALKYYWKFI
                     LKDSRKLSLKPFYARTFRETLTPRECLKKIFTLVPELKDYYDLYQLLLFHLQEKNTDQ
                     FWGLIQDTLPHLNRTFKTTLSTFICYKNYITNAIELPYSNAKLEATNKLIKDIKRNAF
                     GFRNFENFKKRIFIALNIKKERTKFVLSQA"
     gene            complement(6402..>7899)
                     /gene="rlrA"
                     /locus_tag="VVL45_001948"
     CDS             complement(6402..>7899)
                     /gene="rlrA"
                     /locus_tag="VVL45_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PI-1 pilus transcriptional regulator RlrA"
                     /protein_id="HEU4188075.1"
                     /translation="DKITILNILLDIRSIELDELSTLTSLQSKSLLSILQELQETFEE
                     ELTFNLDTQQVQLIEHHSHQTNYYFHQLYNQSTILKILRFFLLQGNQSFNEFTQKEYI
                     SIATGYRVRQKCGLLLRSVGLDLVKNQVVGPEYRIRFLIALLQFHFGIEIYDLNDGSM
                     DWVTHMIVQSNSQLSHELLEITPDEYVHFSILVALTWKRREFPLEFPESKEFEKLKNL
                     FMYPILMEHCQTYLEPHANMTFTQEELDYIFLVYCSANSSFSKDKWNQEKKTHTIQLI
                     LQHTRGKHLLSKFKNILGNDISNSLSFLTALTFLTRTFLFGLQNLVPYYNYYEHYGIE
                     SDKPLYHISKAIVQEWMTEQKIEGVIDQHRLYLFSLYLTETIFSSLPAIPIFIILNNQ
                     ADVNLIKSIILRNFTDKVASVTGYNILISPPPSEEHLTEPLIIITTKEYLPYVKKQYP
                     KGKHHFLTIALDLHVSQQRLIYQTIVDIRKEAFDKRVAMIAKKAHYLL"
BASE COUNT         2341 a         1470 c         1604 g         2484 t
ORIGIN      
        1 aatctatgta tcttattgaa attttaaaat ctatcttctt cggaattgtt gaaggaatta
       61 cggaatggtt gccgatttcc agtacaggtc acttgatttt agcagaggaa ttcatccaat
      121 accaaaatca aaatgaagcc tttatgtcca tgtttaatgt cgtgattcag cttggtgcta
      181 ttttagcagt tatggtgatt tattttaaca agctcaatcc ttttaaaccg accaaggaca
      241 aacaggaagt tcgtaagact tggagactat ggttgaaggt cttgattgct actttacctt
      301 tacttggtgt ctttaaattt gatgattggt ttgataccca cttccataac atggtttcag
      361 ttgctctcat gttgattatc tacggggttg ccttcatcta tttggaaaag cgcaataaag
      421 cgcgtgctat cgagccaagt gtaacagagt tggacaagct tccttatacg accgctttct
      481 atatcggact cttccaagtt cttgctcttt taccagggac tagccgttca ggtgcaacga
      541 ttgtcggtgg tttgttaaat ggaaccagtc gttcagttgt gacagaattt accttctatc
      601 ttgggattcc tgttatgttt ggagctagtg ccttaaagat tttcaaattt gtgaaagccg
      661 gagaactctt gagctttggg caattgtttt tgctcttggt cgcgatggga gtagcttttg
      721 cggtcagcat ggtggctatt cgcttcttga ccagctatgt gaaaaaacac gacttcaccc
      781 tttttggtaa ataccgtatc gtgcttggta gtgttttgct actttacagt tttgtccgtt
      841 tatttgtata agaaaaacct tgaaggggca actcttcaag gttttatact cttcgaaaat
      901 ctcttcaaac cgcgtcagct ttatctgcaa cctcaaaaca gtgttttgag cagcctgcgg
      961 ctagcctcct agtttgctct ttgattttca ttgagcttta aaatccagtc atggtaatcc
     1021 ccaataggcg gacacctctt tctttcttgc ttaattcttc atagagttgc agggctattt
     1081 ggcttatctg actagcatct tgtgtttttt gagcaagact ttttcgtttg gtaagagttg
     1141 aaaagtcctc gtagcggatt ttcaaaatga caatttttcc agctttttct tgttgatgta
     1201 gattgagagc gactttttct gatagaagag tcagctcttt tttgatatct tcctcagcaa
     1261 ggagaatctt cccgtaggtt ttctccttgc cgattgattt acggatgcga ttggatttga
     1321 ctggagagtt gtgaatgcca cgagcctttc gatacagatc atagcctagt ctaccaaaac
     1381 ggtctattag ggttacctca ggaacttcaa gtaaatcagc accagtaaaa acgcccattt
     1441 gatgaagacg ttctactgtc ttttttccta ctccatgaaa tttggaaata tccatttgtt
     1501 tgagaaaatc ctcagcctgt tcaggtagaa tcactgtcaa accatgtggt ttttgataat
     1561 cactcgccat tttagctaag aatttgttgt aagaaacgcc tgcggaagca gttagatgga
     1621 gttcttgcca gatatctttt tgaatgaggc gagcaatttt gaccgctgac ttgataccga
     1681 gtttattttc tgtcacatcc aaataggctt cgtcaatgct catgggttca atcaaatctg
     1741 tatagcgctt aaaaatagct cgaatctgga gtcccacaga cttgtatttc tcataattcc
     1801 ctgagataaa gacagcctgg ggacaacgtt cataagcttc cttggaactc atggcagaat
     1861 ggacaccaaa agctcttgcc tcataactac aggtagaaac gactccccgt ccacctgttt
     1921 gccgagggtc gcttccaata atgacaggtt ttcctctgag tttaggatta tccctgattt
     1981 ccactgcagc aaaaaaggca tccatgtcaa tatggatgat ttttcttgac aaatcattta
     2041 acaaaggaaa aatcaacatg cctagcacct ttttatactc ttcgaaaatc tcttcaaacc
     2101 gcgtcagctt tatctgcaac ctcaaaacag tgttttgagc aatctgcggc tagcttccta
     2161 gtttgctttt cgatttccat tgagtgttac tgcttatttt cttttattat accctttttt
     2221 ctgaaaaaaa gaaaaaagga ctttattttt tcaaaaatat aatacagttt gaaataaaat
     2281 atagactgtt ttagaaaaga aagtgtaaaa atagggaatt ttcacttgtt gaaatcggtt
     2341 actatatggt atacttgtct tatgaatgta acagatgact gttactagaa aaaagaggac
     2401 attaatatgg ttgttaagac agttgttgaa gcacaagata tttttgacaa agcttgggaa
     2461 ggcttcaaag gcgtagattg gaaagaaaaa gcaagtgtat cacgatttgt acaagctaac
     2521 tacacacctt atgatggaga cgaaagcttc cttgcaggac caacagagcg ttcacttcac
     2581 atcaagaaaa ttgtagaaga aactaaagca cactacgaag aaactcgttt cccaatggac
     2641 actcgtccaa catctatcgc tgatatccct gctggattta tcgacaaaga aaatgaagtt
     2701 atcttcggta tccaaaacga tgaactcttc aaattgaact tcatgccaaa aggtggtatc
     2761 cgtatggctg aaactacttt gaaagaaaat ggatacgaac cagacccagc tgttcacgaa
     2821 atcttcacta aatatgtaac aacagttaac gacggtattt tccgtgccta cacttcaaat
     2881 attcgtcgcg ctcgtcacgc acacactgta actggtcttc cagatgcata ctcacgcgga
     2941 cgtatcatcg gtgtttacgc acgtcttgct ctttacggtg cagactactt gatgcaagaa
     3001 aaagtaaatg actggaatgc aatcaaagaa atcgatgaag aaacaatccg tcttcgtgaa
     3061 gaagtaaacc ttcaatacca agcattgcaa caagttgttc gcctgggtga cctttacggg
     3121 gttgatgttc gcaaaccagc gatgaacgtg aaagaagcaa tccaatgggt taacattgct
     3181 ttcatggctg tctgccgtgt gattaacggt gctgctacat ctctaggtcg tgtaccaatc
     3241 gtattggaca tctttgcaga acgtgacctt gctcgtggta catttactga atcagaaatc
     3301 caagaattcg ttgatgattt cgttatgaaa cttcgtacag ttaaatttgc tcgtacaaaa
     3361 gcttatgacc aattgtactc aggtgaccca acctttatca caacttctat ggctggtatg
     3421 ggtaacgacg gtcgtcaccg tgttactaag atggactacc gtttcttgaa cactcttgac
     3481 aacatcggta actcaccaga accaaacttg acagttcttt ggactgacaa attgccatac
     3541 aacttccgtc gctactgtat gcacatgagc cacaaacact cttctatcca atacgaaggt
     3601 gtaacaacaa tggctaaaga cggatatggt gaaatgagct gtatctcatg ctgtgtgtct
     3661 ccacttgatc cagaaaatga agaacaacgc cacaacatcc agtacttcgg tgctcgtgta
     3721 aacgttctta aagcccttct tactggtttg aatggtggtt acgacgatgt tcacaaagac
     3781 tacaaagtat ttgatatcga accaatccgt gacgaagttc ttgaatttga atcagttaaa
     3841 gcgaactttg aaaaatctct tgactggttg actgacactt acgtagatgc cttgaacatc
     3901 atccactaca tgactgatag gtacaactac gaagctgttc aaatggcctt cttgccaact
     3961 aaacaacgtg ccaacatggg attcggtatc tgtggatttg ctaacactgt tgatacattg
     4021 tcagctatca aatacgctac agttaaacca atccgtgacg aagatggcta catctacgat
     4081 tacgaaacaa tcggtgacta cccacgctgg ggtgaagatg acccacgttc aaacgaattg
     4141 gcagaatggt tgatcgaagc ttacacaact cgtctacgta gccacaaact atacaaagac
     4201 gcagaagcta cagtatcact tttgacaatc acatctaacg ttgcttactc taaacaaact
     4261 ggtaactcac cagttcacaa aggtgtatac ctcaacgaag atggttctgt gaacttgtct
     4321 aaacttgaat tcttctcacc aggtgctaac ccatctaaca aagctaaagg tggttggttg
     4381 caaaacttga actcactttc tagccttgac tttagttatg cagctgacgg tatctcattg
     4441 actacacaag tatcacctcg cgctcttggt aagactcgtg atgaacaagt tgataacttg
     4501 gtaacaattc ttgatggtta cttcgaaaac ggtggacaac acgttaactt gaacgttatg
     4561 gacttgaacg atgtttacga aaaaatcatg tcaggcgaag acgttatcgt acgtatctct
     4621 ggatactgtg taaacactaa atacctcact ccagaacaaa aaactgaatt gacacaacgt
     4681 gtcttccacg aagttctttc aatggatgac gccttggatg cattgagcta atcaagttct
     4741 tgaataataa aaaggaaccc tcggtcaaac gactgagggt tttgtgcttg ggatagtatg
     4801 agcaattcct tcggcgcaat atgcaatgtt tttgggctct ttgtcaactg tagtgggttg
     4861 aaaaaaagct aagcttgaga aaggacaaat ttcgtccttt cttttttgat gttcagggcg
     4921 ataaaaatcc gttttttgaa gttttcaaag ttccgaaaac caaaggcatt gcgcttgatg
     4981 tctttgatga gtttgttagt ggcctcaagt ttagcgttag aataaggcaa ttcaatggcg
     5041 ttagtgatgt agtttttata gcaaataaat gtgctcaaag tggttttaaa ggtgcggttg
     5101 agatgaggta acgtgtcttg aattaagccc caaaactggt cagtattctt ctcttgtaga
     5161 tgaaatagga gtagttgata caggtcatag taatctttaa gttcaggtac tagagtaaag
     5221 attttcttca gacactccct aggagttaag gtctctctga aagttctagc atagaaaggc
     5281 ttaagagaga gtttccgact atcttttagg ataaatttcc agtaatattt aagagctctg
     5341 tattccagag atttatcatc aaattgcttc atgatgttga ttctagtctg attaagagcc
     5401 ctgctcatgt gttggacaat gtggaaacga tcgagaacaa ttttagcatt gggaaataat
     5461 ttcttaatga gagggatata acttccagac atatcaacag tgacgacttt aacttttttt
     5521 ctagcttctt tcgagtactt gaagaaatga tttcggatgg ttgtttgacg tctgttatca
     5581 agaatggtca tgattttctt agtgttgaaa tcctgagcaa tgaaagccaa tttccccttc
     5641 tggtaggaga attcatccca ggagaggatt tcaggcaaag tggtgtaatc ctcttggaaa
     5701 tgaaattgct tgagcttacg atagacggta gaggtagagg tagaggtaga gatggctaat
     5761 ttagaagcga tatgtgtaag agcctctctg ttgagtagga gttgggcaat tttctgtctc
     5821 accatttccg agatttggca atttttctga acgagagttg tttcagctac agtgactttc
     5881 cgacaggact tgcattgaaa tcgtctcttt ttcaaatgaa tgaggctagg gaaaccacca
     5941 atctcgataa aagggatttt agaaggcttt tggaagtcgt atttgatttg ttttccttta
     6001 cagtgtttac atttaggtgg gtgataatca agtgtagcga agacttcgat atgggtatcg
     6061 tgctgaatgg ctttatttaa ggtgatgttt ttgtctttta ttccgatgag taatgtggta
     6121 tgattgatgt gttccataag atactttcta atgagttgtt taggcgcttt tcattataag
     6181 tcttatggga cttttttgat actcaaaaag ccctataatc tccacagtgg gatttaccca
     6241 ctacagaaat tatagagcca gaaaaaacac ttttgttcac tagcagaaac tagagagcag
     6301 aagtgttttt ctgttcagat ttacccaaaa ctgggaaata tggggataag aatagagatg
     6361 gcttaggaag cccctttttg tgtgtagaca gtacgatgaa cttataacaa atagtgagcc
     6421 tttttagcaa tcattgcgac ccgtttgtca aaagcctctt ttcggatatc tacaattgtc
     6481 tgatagatga gacgctgttg gctaacatgc aaatctaagg caatcgtcaa aaagtgatgt
     6541 ttccctttgg gatactgctt tttaacgtaa ggcaggtatt ctttcgttgt aataataatc
     6601 aatggctctg tcaaatgctc ctctgaagga ggaggactaa ttagaatatt gtatcctgta
     6661 acagaggcaa ctttgtcagt aaaattccgt aaaataatgg actttattaa gtttacatct
     6721 gcttgattat ttaaaatgat aaaaatcggg atagcaggta gtgaggaaaa gatggtttct
     6781 gtcaagtaga gtgagaaaag gtacagccga tgctggtcga taactccttc aatcttctgc
     6841 tcagtcatcc actcttgaac aattgctttc gaaatatgat acagtggctt gtcgctttca
     6901 atcccataat gttcgtaata attataatag ggaactagat tttgtaaacc aaacaaaaac
     6961 gttcttgtta agaaagtcag tgctgttaaa aaagaaagag aattcgaaat gtcatttcct
     7021 aagatattct tgaacttgga tagtagatgc tttcctcttg tatgctgaag aatcagttga
     7081 atagtatgag tctttttttc ttgattccat ttgtccttgg aaaacgaaga attagcagaa
     7141 caataaacca aaaagatata atccagttct tcctgagtaa aagtcatgtt ggcatgtggc
     7201 tctaagtaag tttggcaatg ttccatcaaa atcggataca taaagaggtt ttttaatttt
     7261 tcaaactctt tggactcagg gaactcaagt ggaaattccc gacgtttcca agtgagtgcc
     7321 actagtatgc taaaatgaac atactcgtca ggtgtgattt ctaacagttc atgactgagt
     7381 tgagaattag actgcacaat catatgtgtg acccaatcca tacttccatc attcaaatca
     7441 taaatctcaa taccaaaatg aaactggagg agtgcaatta aaaaacgaat gcgatattca
     7501 ggaccaacta cttgattttt cacaaggtcc aaacctactg aacgtagtaa caagccacac
     7561 ttttgtcgta cgcggtagcc tgttgcgatg gaaatatact ctttttgtgt aaattcgtta
     7621 aagctttgat taccttgtag tagaaagaag cggagtattt ttaaaatagt tgattggtta
     7681 taaagctgat ggaagtaata attcgtttga tgagaatggt gttcgattaa ttgaacttgt
     7741 tgcgtatcta aattaaatgt caactcttcc tcgaatgttt cttgtaattc ctgcaaaatg
     7801 cttaggagac ttttagattg taatgaagtt aaagtagaca gttcatctag ttcaatagac
     7861 cgaatatcca ataatatatt taaaatggta attttatct
//
LOCUS       DAVHEX010000079         7763 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVHEX010000079 DAVHEX010000000
VERSION     DAVHEX010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7763
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.080"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..151
                     /locus_tag="VVL45_001949"
     CDS             <1..151
                     /locus_tag="VVL45_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU4188076.1"
                     /translation="KKMTPKEVAFIEKWINNYPKKCLDYKSPREDFWMANLNLKFSVR
                     NKSRN"
     gene            177..821
                     /locus_tag="VVL45_001950"
     CDS             177..821
                     /locus_tag="VVL45_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEU4188077.1"
                     /translation="MLRAGVLDANDGIISIAGVVIGVASATSNIWIIFLSGFAAILAG
                     AFSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYASYIQNGEFKTSAQLL
                     TNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSDYR
                     IPATVLIVGVALLLTGYTSARLGKAPTKTAMIRNLAIGLLTMGVTFLLEQLFSI"
     gene            complement(863..2851)
                     /locus_tag="VVL45_001951"
     CDS             complement(863..2851)
                     /locus_tag="VVL45_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4188078.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTITYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVLFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQMKVVITVLL
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVTDPLTALTTFFLAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSAESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYSVKEKEVLRYSNFGIANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNDGLKGQKALTLNDHQFSVKEEFNKDFIVNHVPNKF
                     NILTTDYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVDASEEEQLKVAEEYENYLNQF
                     NAQLDTEGSYVYGSTLADASAQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTIHKQVLTVFFLPLLFAFIHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(2853..3611)
                     /locus_tag="VVL45_001952"
     CDS             complement(2853..3611)
                     /locus_tag="VVL45_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4188079.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFNEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            4039..4230
                     /locus_tag="VVL45_001953"
     CDS             4039..4230
                     /locus_tag="VVL45_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188080.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFISTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
     gene            complement(4584..4856)
                     /locus_tag="VVL45_001954"
     CDS             complement(4584..4856)
                     /locus_tag="VVL45_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU4188081.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(4914..5363)
                     /locus_tag="VVL45_001955"
     CDS             complement(4914..5363)
                     /locus_tag="VVL45_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEU4188082.1"
                     /translation="MKLTDYVKQVSLEDFGRPFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQELGLDVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPIKLYRCQSCQQRYQRKRRIDTQRYRCGLCRGKLLLVNQPED"
     gene            complement(5350..7479)
                     /locus_tag="VVL45_001956"
     CDS             complement(5350..7479)
                     /locus_tag="VVL45_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEU4188083.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRAQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYTAVKELFKRL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVSVWVKKIDTEREKVNLSLLAPNETD"
     gene            complement(7581..>7763)
                     /locus_tag="VVL45_001957"
     CDS             complement(7581..>7763)
                     /locus_tag="VVL45_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1919 domain-containing protein"
                     /protein_id="HEU4188084.1"
                     /translation="NKMDNLPYSNKLILVNRPYKNLKSQCVIPGQEHLPELAIMSDPL
                     NTFDIMAWLKKGGNAL"
BASE COUNT         2322 a         1622 c         1500 g         2319 t
ORIGIN      
        1 caagaaaatg actcccaaag aagtcgcatt catcgaaaag tggattaaca actatcctaa
       61 aaaatgcttg gactacaagt cacccagaga agacttctgg atggctaact tgaacttgaa
      121 atttagcgtc agaaacaaat cacgaaattg attcaaattt tgcaggtcgt ttaaatatcc
      181 tgcgtgcggg tgttcttgat gctaacgatg gaattatttc cattgctggt gtggttatcg
      241 gagttgccag tgccacgagc aatatctgga ttatcttttt atcaggtttt gcggctatct
      301 tagctggtgc cttttcaatg gctggtggag aatatgtatc cgtttcaact ccaaaagata
      361 ccgaggaagc tgccgtttcg cgagaaaaac tcttgctaga ccaagatagg gaactagcca
      421 aaaaatccct ctatgcttct tatatccaaa atggagaatt caaaacttct gcccaactct
      481 tgactaataa gatctttctt aaaaatccac tcaaggctct ggtagaggaa aaatatggga
      541 ttgagtatga agaatttacc aatccttggc acgctgccat ttctagcttc gttgcctttt
      601 tccttagaag tttgcctcca atgctgtcag tgaccatttt tccaagtgat taccgcatcc
      661 ctgctaccgt ccttattgtc ggtgtggccc ttcttctcac tggttacaca agtgctagac
      721 ttggaaaagc cccaaccaaa acagctatga ttcggaacct tgctattggt ctcttgacca
      781 tgggagttac cttcctgctc gaacaacttt tcagcattta aaatacaaga aatacctcga
      841 ttttgaagtc gaggtatctt ttttacattt gcacaatctt gcgataactt cttgaagtaa
      901 tcatgaaaat cagcacatag gcgatgagga agatagcgca gatagacaag gtcacaatca
      961 acatcatagt cgtatccagt acaccaatca cttttaaaat caggctaagc atatggtagg
     1021 caaaggcgag atgtatgaag gcaaagagca aaggaaggaa gaaaacagtt aaaacctgtt
     1081 tgtggatggt ttgcttgatt tgcttttggt ccaaaccgac tttctgcaag ataataaagc
     1141 gttcacggtc ttcgtagcct tcagaaattt gtttgtagta gatgaccaga acagttccga
     1201 ccataaagat aatggatagg aaaataccga taaagaagac accaccaaag agggcactca
     1261 tctgagcact agcatctgct agagtgctac cataaacata gctaccttct gtgtctaatt
     1321 gagcattaaa ttgattgagg tagttttcat actcctcagc gaccttgagt tgttcttctt
     1381 cgctggcatc tacattcata ccaccgtaaa actgattata gatagccgaa tctgggaatt
     1441 gatccaaaaa ggcttgtaaa tcaggaacaa caaggtaatt gtaatcagta gtcaagatat
     1501 taaacttatt tggaacatgg ttgacaatga aatctttatt aaattcttct ttgacagaaa
     1561 attgatgatc atttagagtt agagctttct gtcctttcag tccgtcattt ttggcaaaga
     1621 gaccgacctc atttcctgat agagacagtt tttgaccagt catgttttca taatcttttt
     1681 ggtcaaatac catgaaaact gtttttggtt ggacacggtt ttgtcctttt tcaaaaatag
     1741 ttaacttggt tccttcttga tttgcaatac caaagttact gtaacgaagt acttctttct
     1801 ctttgacact ataacctttg tcacttgcaa actggctcaa gagtttgtcc aaatcttctt
     1861 tttcaacatt ttgccctgaa accccaaaat catgaggatt tagaactttt ttaaaggatt
     1921 ctgcggaatt gaaaatgctt gtcgctgctg acatggttac caaaaccatt gttgacaaaa
     1981 tagcgatggt tgctagtcca accgcatttt tcttcatacg gaaaatcaag ttggaaacag
     2041 atatgaggtt attaggttgg taatagtatt tcttgttttt ctttaagatt tgtaggaaga
     2101 ctgtaatccc tgcattaaac aatagataag taccaaagat aaccagcaaa acagctagga
     2161 agaaggttgt tagggctgta agaggatcgg ttaccgtaag ggcaagataa tagccaatcc
     2221 ctaaacttat ggaaccaaga atcgtttgga gaggaaggaa gcgacctttt ttctctccgc
     2281 tagctttctc acgtgagagc tggagggcat tcatacgggc gattcgaagg gcattcagga
     2341 acatgaggcc taggaaaatc aatccaaaga caagaagtac tgtaatgaca actttcatct
     2401 ggaaggtagc gaccagttcc accttcaatt tcatcagttt gagtaggaaa gcgaaaatca
     2461 acttgtcaaa caaggctcca ataccgattc ctgctccaac agttagaatc ccaaatagca
     2521 ctaactcctt aaaggtcata ctgattagat ggcgcttctc caagcctaac atgccatata
     2581 tacccagttc cttggaacgg tttttcatga caaaactatt ggcatagagg acgataatgg
     2641 ctgacgcaag ggtaacgaca aacataccaa atccaagtgt tgcttgaatg gtggttcctc
     2701 cacggatttc cgcaatcttt ggattgaagg ttagggagta aaagagatag gtgatggtga
     2761 ctgccaagag aacagccagt gcaaagggat agtagagttt gcggttttta atcaagttcg
     2821 ataccgctaa cttattggtt aatcgaaaca tactaattca cctcgcttgc catgacagtc
     2881 aaggtatcag agatttcttg gaacatctga cgctctgtct tctctccacg gtagatttgg
     2941 ttgtaaagaa tgccgtcttt gataaagaga acacgcttgg ccctgctagc agctgctgtt
     3001 gagtgggtta ccatgaggat ggtttgccca cgctcattga tttcattaaa gacatcaagt
     3061 aaggctgcag atgacttgga atcaagggct cctgttggct cgtccgcaag gagaatttca
     3121 ggttctgtga tgatggcgcg ggctactgct acacgctgtt tctgaccacc agaaatctcg
     3181 taagggtact tctcttgcaa ttggttaata cccagattct cagctgtcac caccaatttc
     3241 ttcatcatct ccgttatagg tcttcttgac aagacaagcg gaagcaagat attgtcctta
     3301 acagacagag tatctagcaa gttaaagtct tggaagacaa atcctagctt ttcacgccgg
     3361 aaactagaag cctgtgaatt tttaatagtt gcggtgtcag ttccattcaa gtaaacctga
     3421 ccacgacttg gtttatccaa catagctaga atattgagaa gagttgattt accagaacca
     3481 gactcaccca tgatggcaac gtagtcaccc ttttctacgg taaagtgaat atccttgagg
     3541 gcttctactt ggttgccctg aaaacgtgtt ttataaattt tttgaacgtg ttttacatct
     3601 aaaagtgtca tgaaaatctc ctttcttaat atcccatcaa atgaaatgta gcttgcatca
     3661 tagtgcatcc cagctgaacg cgctcaattt ctttgtgact tggttttcta gttttcttat
     3721 gtaagatttg tttcatgatg ttactccttt gttctttatg agtctagttt acatcaaaaa
     3781 aagaccgctt gccataacct aaccttacaa aagagacttt aatcttacat ttttgtaaga
     3841 ttgggtgaaa tataagcaaa attacttaga aaatgatttg atttcctatt tttaaagttg
     3901 taaaatataa gtgtgaaaag gcttactttg aaaaggagga agcgggaact aacattttag
     3961 taagcttacc tagataacat accattcaat ttaacacttg caagtcgttc aacctataag
     4021 gaaagaaggt gcgctttaat gaaacacaaa gaacatatac tcatcggact tctctatctg
     4081 ctctctccat tcatcggtca gctcttagtt gaacacacac actttatcag tacagaattt
     4141 accggcactg cttatgttat ttgctggtta tcagttgtta tcagcatcca ccatttttcc
     4201 aaaaatgtat tgtctcaaca gcaaaaatag attaaaaaac tgcgtttaaa ttactgcttt
     4261 tatagtaaaa tgaaataaga acaggacaaa tcgatcagga cagtcaaatc gatttctaac
     4321 aatgttttag aagtataggt gtcctattct agttccaatc cactatagtt tgtggatgaa
     4381 gctggcgaaa tattattaat actattcaaa aatcaaagtg caaactatat caacttcaca
     4441 ttgccgacta aattacagcc tgcggctggc ttcctagttt gcactttgat ttttattgag
     4501 tataaaagta aaaaagcttt aaatcaagca gtttagacta ttgatttaaa gctttttgcg
     4561 tattccaaat cttttgtaaa acctcaccaa aaccagcctt cattatcgtc aatggcttga
     4621 gcttctttgc gtttacgtgg gcctgttccg tacatttctg cttcgatttc ttccttggtt
     4681 ggcatgatag aagctaggat gatatagata atcaccccaa gtccaaagtt tgcgactgta
     4741 aaaatggcaa acagaaaacg tacaagggta acatcaaagt tccacttgtc tgacagacct
     4801 gctaaaactc ctgaaatcat gcgatttcgt ctcattttat aaaacttact gttcatgata
     4861 tttcctcttt ctgctaggtt agactagtat atcacggata ggctcggctt tcatcagtcc
     4921 tcaggctgat ttactaatag caactttcct cgacaaagtc cacagcgata gcgttgggta
     4981 tcaatcctac gcttacgctg atacctttgc tggcaggatt ggcaacgata gagcttgatt
     5041 ggcttggagt tactattggg caaggatggt acaaaccgta atccatccac tgctttcaac
     5101 agttccttaa aatcccgatc cttgtgttga tagcctttcc cttgaaaata gaggtgataa
     5161 tgacagagtt catgtcggac aattttccta aaaacgtcca accccagttc ctgataaacc
     5221 ttgggattaa aatccaaatg cccatctttg gggaaaaatc gcccacctgt cgaacgtaga
     5281 cgcctattcc actggacatg atggataaaa ggtctgccga agtcttctag tgaaacctgc
     5341 ttgacgtaat cagtcagttt catttggagc taggagagac agattaactt tttcacgttc
     5401 agtatcaatt ttcttaaccc aaacgctcac caaatctcca actgccacca cttgactagg
     5461 gtgtttgata aacttgcgac tcatatggga aatatggatg agaccgtcct catgaattcc
     5521 gatatcaaca aaagcaccga aatcaacaac gttacgcacc actccttcta gcttttgtcc
     5581 aaccactaag tccttgatat ctaggacatc ttggcgaagc acaggtgcgt caaaggaatc
     5641 acggaaatct cgacctggtt tgagaagatc tgcaatgata tctttaagag tttctggacc
     5701 aaggtctaac tcttgcgcca tttccttgac tgaaagcgac ttgagtttgc tttgggcttc
     5761 ttcgtttagg tctttaatat ctaaacgttt gaagagttcc ttaactgcag tgtaattctc
     5821 tgggtgaact cctgtattat caaggatatt gctactttca gggatacgaa ggaaaccagc
     5881 agcctgctca aaggccttgg ctcccagacg aggaactttc ttgatttggg cgcgtgaagt
     5941 gatttttcct tcttcctcgc ggtatttgac aatattttca gagatagttt tgttgagtcc
     6001 agctacgtgt gaaagaagag ctgggctagc tgtattgaca ttgacaccaa cttggttaac
     6061 cactgtatcg acaacaaagt ccagactctc agatagtttc ttctgactga catcgtgttg
     6121 gtattgaccg acaccaattg acttaggatc gattttgacc aattccgcaa gaggatcttg
     6181 caaacgacgg gcgatagaaa tggcagagcg tttttcaacg gtcaagtctg gaaactcctg
     6241 acgagcaagt tcgctggcag aatagacaga agcaccactt tcattaacga taacatagct
     6301 gacttcaggg aaatctttca gaacttccgc tacaaaagct tcactttcac gactggccgt
     6361 tccatttcca atggcaataa tctctacacc gtattgacca attaaatctg ctaaatcttt
     6421 cttggcttct tcgatttgac gagctgatgc tggtttaaca ggataaataa cctgagttgt
     6481 cagcattttt cctgttgcat ccacgacagc tagcttggca cctgtacgaa aggctgggtc
     6541 aaatccaaga accacgcgcc ctttcagtgg agcaaccaag aggagattgc gcagattgtc
     6601 agaaaaaagt tggatagctc cttcttcagc tttctcagtt aattctgtcc gaatacgacg
     6661 ctcgatagca ggcaagacct ttttcttaac ggattgctga acaacttcat caatataagc
     6721 atttttcacc ttgaaacgag tagcaaagaa ggcaagaata cggtccgtcg catgttcaaa
     6781 accgatcttc aagacaccaa gtttctcccc acgattgaga gccaaggtac gatagccttg
     6841 catagttcca actgtctctg aaaaatcata ataaatctga aaaacctgct tttcatcaag
     6901 actttcatcc ttggcttgag aagtaagttt agagtgtctc agcacttcct gataagtcat
     6961 agaacgcaag gtcacatctt ccgataaggc ttcgaccaaa atatcaactg caccggtcaa
     7021 ggcttccttg ccagtcgcaa atccttcaca gacgaacttt tcagcttctt tctctaagtc
     7081 aactatattc tgcaaaatca agcgagcaag aggaaagagt ccagcttcac gggcaatggt
     7141 tgccttggta cgacgctttt ccttataagg aagatagagt tcttcaacgt ctgctaattt
     7201 ttcggcaact aagatagctt cttccaattc cttggtcaac ttaccttgtt cttgaatctt
     7261 agctaagaca gcttccttac ggtcattgag atttgtcaga cttttatcca aatcaataat
     7321 agccttaatc gccacctcat ccagactacc agtcatgtcc ttgcgataac gcgcgataaa
     7381 gggaatagtc gccccttcag ctgtcaaact tagaacggta tcaatttgct ttaacgtcac
     7441 tcccaaatcc tgagagattt tttcatattt tttatccata aatctattat accacaagct
     7501 aaacgtttca aattaactcg tagaacattt aaaaaatatg taggaaatag atttatatgc
     7561 tacagcgcaa taacttgcac ttaaagagca ttgccacctt tttttaacca agccatgata
     7621 tcaaaagtat ttaatggatc agacataata gccagttctg gaagatgttc ctgacctgga
     7681 ataacacatt gacttttcaa atttttatat ggacgattga ctaaaattaa tttattagaa
     7741 taaggaagat tatccatctt att
//
LOCUS       DAVHEX010000080         7047 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVHEX010000080 DAVHEX010000000
VERSION     DAVHEX010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7047
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.081"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            46..906
                     /locus_tag="VVL45_001958"
     CDS             46..906
                     /locus_tag="VVL45_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188085.1"
                     /translation="MVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQEYCQGQPY
                     GLLKKMLKRIRLSREIALLLATIIVYFFTHRILPLSVFTFMFSYILLFVQSYLGSNTA
                     WIGNRRLIIDDEFEKILLSKSYVKEISSARYSEYLTCEYKNLTPIILIAIFENLLDSY
                     LLQNQSEVDLDIFYKVLPLLYKEKYTMGFNYFVSLNYLLYKVGFLGIIYDNEALRDLS
                     KQYLNKNISELQDGSFEGGIQDAVASKQIVVINEFIACLNSKCMPSQYDRFFYKDRPY
                     IFSRKSSIKG"
     gene            1221..2198
                     /locus_tag="VVL45_001959"
     CDS             1221..2198
                     /locus_tag="VVL45_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188086.1"
                     /translation="MKNKRYFFDTILMILLLISTIFCVSPVFIKLDILGTPSHAILTF
                     VLAIPLFYILSQCLHTLLLLVSSIFCKLRPIYFHFIFVIIIGARKYYRILFHQLMGFS
                     PGVAVFYKESQTTKNLFKFYYFLYFTTLISHYFFFTFVYDKPLLLPLIPFSIIIALVQ
                     KLYRIENQQLFLLKSKVLTILESKKNCEFNLQDYHEIWKLQSKSELPCVALSYISLIK
                     PYLSESVREQIDLLEVKRFKKINHPISLYGMLDVIKLNLYLRHYNEKNKYESMLKKIL
                     EVRPDFVLIEQNIDDSLNSSQPLSLSLAISEIQLLLEVYMGIKHVSIRR"
     gene            2575..2949
                     /locus_tag="VVL45_001960"
     CDS             2575..2949
                     /locus_tag="VVL45_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HEU4188087.1"
                     /translation="MELVLPNNYVVLEQEEMMYLDGGFSIPRWPVATAINIAFNGVLG
                     GGAISLVRNYIRNYGLRRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGN
                     AITTIWANNDVNRRDPNLNALW"
     gene            3157..4059
                     /locus_tag="VVL45_001961"
     CDS             3157..4059
                     /locus_tag="VVL45_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188088.1"
                     /translation="MIRKLIIFLLMLPLFVLWLELHLLVSNLQLNLEIPFDFVVSTSL
                     TFFVLILSKIVLDILYALKDLYKKEALITIFPFIFIGRKKVNVRFSPYFSFHRKSLSP
                     DDLRSRIIWSFILEIAIILVFILKIPFAIIMLTTIFFWTIMDINHLVFNKTEFLFNQN
                     KWQKEDSFESDLTKTLKDKIQKSELSYSDLMSLLLYDAMNQSTFLTDSELFEDILKKI
                     EDSHNTLLCTGLVELLLYEISISKNHNWSEKIDKIRIELIKINQLDFFYYTSWLRQNF
                     DFCMNREYHKMKSRKLLLSNKKIV"
     gene            4115..4468
                     /locus_tag="VVL45_001962"
     CDS             4115..4468
                     /locus_tag="VVL45_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEU4188089.1"
                     /translation="MELVLPNNYVVIDEEVMMYLDGGAYLSKRACQGICAALAMSSGT
                     FIALAGAAVLTKKLINYIKFGGLGGWLIGAAAGVLATAAGKIAYCIGYGVLNRGCDIN
                     GNPYPWDGFISATVR"
     gene            4465..5193
                     /locus_tag="VVL45_001963"
     CDS             4465..5193
                     /locus_tag="VVL45_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188090.1"
                     /translation="MSNVDKIRKIHIIVCWVYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLAINSILLISFPILINKFFPESFLTYTVLISVFITELIIFHL
                     IGKDFDTKLTNEYKKISQFRSKVSQSPWIKYLEISSFILTIFPSILYGTVDNHVLTLI
                     FLIKICVDTTIKFLFIRLFDTSTLMKRRIFFLFALDVIAYLFLGYLLVIQKAGYLFSI
                     LLLFSNFSVPFIKEKEYELFKNSK"
     gene            6325..6564
                     /locus_tag="VVL45_001964"
     CDS             6325..6564
                     /locus_tag="VVL45_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188091.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLEIFKVNFCLTDLHHLF
                     DLHKITRDYASQTKPAIQDGVFILEDFRNILCTMM"
BASE COUNT         2364 a          906 c         1238 g         2539 t
ORIGIN      
        1 catattgtga taattaagtt tataagaaaa aaatctttta agtatttggt tctatatcca
       61 tttacttatg atgggagctg gagatttcaa cctataaact tactctactt tccagaaatg
      121 gtgagagatg tcattccgat taatttagtt caagaatatt gccaaggaca accttacgga
      181 ctgttaaaaa agatgctgaa aagaattcga ttgtcacgag agatagcttt actcctggct
      241 accattatcg tctatttttt tacgcataga attctacctc tttcagtatt tacgttcatg
      301 ttctcttaca tattgctatt tgtacaatct tatttgggta gcaataccgc ttggattgga
      361 aatagaagat taataataga tgatgaattt gaaaaaatct tgctttcaaa aagttatgta
      421 aaagaaatat catctgcacg atactcagaa tacttgacat gtgaatacaa aaaccttaca
      481 ccaatcatat tgatagctat ttttgaaaat ttgcttgact cctacttgct tcagaatcag
      541 agtgaggttg acttagatat tttttataaa gttttacctt tgctttataa agaaaaatat
      601 actatgggat tcaattactt tgtaagttta aattatttac tatataaggt tggatttttg
      661 ggaatcattt acgataatga agcattaaga gatttgtcaa aacaatattt aaacaaaaat
      721 atttcggaat tacaagatgg ttctttcgag ggcggtatac aagatgctgt tgcctctaaa
      781 caaatagtag ttataaatga atttatagct tgtttaaatt ccaaatgcat gccatcccaa
      841 tatgatagat ttttttataa agatagacct tatatttttt ccagaaagag ttctattaag
      901 gggtgaaaat ttgttacatt tggattaaca agtgtcctag ggattagaaa taactctagt
      961 atagctagat atttttgagt aactagaaat atcattaggc tactcatatg attgccagaa
     1021 aagatggctg atgaatctta tttatactgt tgcagtaggc gttgtctagg ataaataagc
     1081 tatcttgatg ggggagattg gaataatttt agtagaaaca ttaaaggatt atgggcaagt
     1141 agcacaggat taggtgatgc aatgtgctaa gttggcttgg tgttagcagt agtattattt
     1201 aacaatggaa agtagactaa atgaaaaata aacgttattt ttttgacaca attttaatga
     1261 ttttactact tataagcacg atattttgtg tcagcccagt atttataaaa ttggacatct
     1321 taggaactcc ctctcatgcc atcttaacct ttgtattggc gataccgtta ttctatattt
     1381 tgtctcaatg cttacatact ttgctattac tagtttcctc aatcttttgt aaattaagac
     1441 caatttattt tcattttata tttgtgatca ttataggagc taggaaatac tatcgaattc
     1501 tttttcacca actaatggga tttagtccag gagtagcagt gttttataag gagagtcaaa
     1561 caactaagaa tctgtttaaa ttttactact tcttgtactt tacaacacta atttcacact
     1621 attttttctt tacttttgtg tatgacaaac cgttgctttt gcctcttatt ccattttcta
     1681 ttatcatagc attagtacaa aaactttata gaattgagaa tcagcaatta tttctgttaa
     1741 aatcaaaggt actaacaata ctggaatcta agaagaattg tgagtttaat cttcaggatt
     1801 atcatgaaat ttggaaattg caatcaaaat ctgagttgcc atgcgtggct ttatcttata
     1861 tcagtttgat caagccatat ctttcagaga gtgtgcgtga acaaattgat ttattagaag
     1921 ttaagcgatt taaaaaaata aatcatccaa tatcacttta tggtatgttg gatgttataa
     1981 aattaaatct atatttacgg cattataatg aaaaaaataa atatgaatca atgttaaaga
     2041 aaattttaga ggtaagacca gactttgttt taatcgagca gaatattgat gatagtttaa
     2101 atagctcgca accattatct ttatctttag ccatttcaga aatccaactt ttgcttgaag
     2161 tgtatatggg gatcaaacat gtttcaatta ggagataaaa ccagaataat atttgccatt
     2221 ataggggcaa gttgcatatt agtcagtatg tatcttattc cacttattaa aactgagaat
     2281 aaattttggg aaatactttt atttatatta ggtgtattgc taattggaat tgcgttatta
     2341 agtcaaaaaa aataaatttt gaaatagtat tagtgagttc gcaatcattt attgctagtg
     2401 gtttagaact gttaagtttc aaacaataat tgattttaaa ccttaggtaa cctataaaaa
     2461 caaataaggt agagaaagga tatcttatct aggtagctaa gtgataaaaa cacagtcgtc
     2521 tatcacttgt taggaaacta tcctaactat gcttaataaa aaaggagaaa ataaatggaa
     2581 ctcgtattac caaataatta tgttgttctt gagcaagaag agatgatgta tcttgatggg
     2641 ggattttcta ttccgagatg gcctgttgca acagccatta atatagcttt taatggtgtt
     2701 ttaggtggag gagcaatcag tctagttaga aattatattc gtaattatgg tttgcggcga
     2761 gttacaagcg caattgctgg agcagctgca agatatgttg gggtacgagt tgcaaataga
     2821 gtggcaggat ttgcactgtc tgctattaat ggatttgcag cttggatgtc aattggcaat
     2881 gctattacaa caatctgggc caataatgat gtaaatagga gagacccaaa tttaaacgcc
     2941 ttgtggtaac tagatatggg tattaaaatg gaaaaaattt ttgttattat tttttttgta
     3001 tgtctattta tatcatccat aactttttta gcctatgatt ttgttagcga agaaataaaa
     3061 aagttgatta tttggattaa tgtcgttttt ttgattttaa tcatagcaat gataatttat
     3121 ccaaaattaa gaaaatgata aggacactgt aaaattatga tacgtaaact aatcattttt
     3181 ttattaatgc tccctctttt tgtcttgtgg ctagagttgc atttgttagt gagtaacttg
     3241 caattgaatc tagaaattcc ctttgatttt gtggttagta cgagtcttac tttttttgtt
     3301 ttgatattat caaaaatagt tttagacata ctatacgcat tgaaagattt gtacaagaaa
     3361 gaagctctta ttacgatttt tccttttatt tttataggca ggaagaaagt aaatgtgagg
     3421 ttctcgccct atttttcatt ccatcgtaaa agtttgtcgc ctgatgattt aagatctagg
     3481 attatatgga gttttattct tgaaattgct attatcctag tatttatttt aaaaattcct
     3541 tttgcaatca tcatgcttac tacaatattt ttctggacta taatggatat caatcatctc
     3601 gtttttaata agacggaatt tctttttaat caaaacaaat ggcaaaaaga agattcgttt
     3661 gaaagtgatt tgacgaaaac gttaaaggat aagatacaaa aatcagaatt aagctattct
     3721 gacttaatgt cccttcttct ctatgatgcg atgaaccaat ccaccttttt aacggatagt
     3781 gaactttttg aggatatttt gaaaaaaatc gaagattctc ataacaccct tctgtgtaca
     3841 ggtcttgtcg agttactgct gtatgaaatt tctattagta agaatcataa ttggtcagaa
     3901 aagattgata aaattaggat agagcttatc aagattaatc aattagattt tttctattat
     3961 acaagttggt taaggcaaaa ttttgatttc tgtatgaata gagagtatca taaaatgaaa
     4021 tctcggaaat tattactgag taataaaaag atagtttaag ttcgtcctat ttgtaaaaca
     4081 aatgaaacaa tttcaactaa ggaggagaaa acaaatggaa cttgtattac caaataatta
     4141 tgttgtgatt gatgaagaag taatgatgta tcttgatggg ggagcttatt taagcaagcg
     4201 tgcttgtcaa ggaatttgcg cagctttagc tatgagttca ggaactttta tagcattagc
     4261 tggagctgca gttttaacca aaaaactaat aaactatatt aagtttggag gtcttggagg
     4321 ctggcttatt ggtgcagcag caggtgtatt ggctacagca gcaggaaaaa tagcttactg
     4381 tattggatat ggtgttctta atagaggttg tgatattaat gggaaccctt atccttggga
     4441 tggattcata tctgcaacag taagatgagt aatgtagata agattcgaaa aatacacatc
     4501 atcgtgtgtt gggtgtatat atttttatca ttcagggcga ttataaatga tacggagtat
     4561 tttttgctta tttttttagc ttttatctac tcaatagtat cactacctct atattctgtg
     4621 aaaaataaaa tagtatctat ctgtctagcc ataaattcga ttctgttaat cagtttcccc
     4681 attttaatca ataagttttt cccagaaagt tttttgactt ataccgtatt aataagtgtt
     4741 tttattacag agttgataat ttttcattta attgggaaag attttgatac taaattgact
     4801 aacgaatata agaaaattag tcagtttaga agcaaggtgt ctcaatctcc ttggataaaa
     4861 tatttagaga tttctagttt tatattaact atatttccat ctattcttta tggtacagtt
     4921 gataatcatg tactgactct tatctttttg ataaaaattt gtgtagatac tacgataaaa
     4981 tttttattca tcagattatt tgacacaagt actttaatga agaggagaat attttttctt
     5041 ttcgcattgg atgttatagc ttacttattt ttaggatatc ttttagtgat tcaaaaagca
     5101 ggatatctgt tttcgatttt acttcttttc tctaattttt cagttccatt tatcaaagaa
     5161 aaggaatacg aattatttaa aaatagcaag taatagaaac ataggttaaa tcttagaacg
     5221 gcttaaaatc agatatgata aagggaaata gtcttatcta ggtaactaag tgctacaaac
     5281 acaatcgagg atcacttgtt aggattacat tttaatcatg cttaataaga atggagaatt
     5341 cctatgggga ttgtattact ctgaagtttc gaatacctta gttctcgtga ttttgagcta
     5401 ttctaggttt agaatccttc agaatgtaaa attttaagca gtcaaagact tagaatagcc
     5461 tgaaaacatt tattttataa caattctagg cttagaattg ctcatatata tgtatttgag
     5521 ctgctcgata gttaggacag ctttattctg agaaatctga gctatcaatg cttcagaata
     5581 gtatttcgcc aatggttttt attgaagagg tgattgtgtt atgatagtct aaaataaaat
     5641 tgatttaata gagtaaatgg agaatcgtta catgaaacat ttttttgcag gaattggtga
     5701 aataaacttt gtaagttatt tattgtatat atgtgtagga ctagcccctc tttttcatat
     5761 atatgtcata ggttcggaac tgagttttgt gaaaatagtg ttatctattt tgggagttat
     5821 atttgtaagt atgttaacta ttgctcgaat ttatcggact tttctataaa gaatagtttg
     5881 gacttgaact gtatctaaaa acagaggttg gtagattatc agcctctttc ttgtcgttga
     5941 aaagagtgag atatattgac ttttgaaaaa aatgtcataa ttgagttggg ataaacctaa
     6001 aagtaatata gtagattgaa tctggaatag tccgttatga ctgttaaaat atttctagaa
     6061 attaatttga ctttcctaat cgatttgttc acatcttgtt tcaatctact atactttctc
     6121 gttaaaccgt tcggtaattc atggtgacat ggacacatag tagtaagcac actatgtggc
     6181 ttggcagatc taaaaactgt tcccttgcga taagcctaat aagcacaaca tagggagtta
     6241 gagaagccga ctctaatcaa ccactttggg cagtagtggt aactgccccg tgctttttat
     6301 tttgagaaaa tatggagttt gtcgttgaaa ttacttgatt gtattttgga ttatcaagaa
     6361 agattcaatg gaaaaacatg tcaagtatca acgaattata agtatttaga gattttcaaa
     6421 gtgaattttt gcttgactga tttacatcat ttatttgact tacacaaaat cacacgagat
     6481 tatgctagtc aaacaaaacc tgctattcaa gatggtgttt ttattttaga agattttaga
     6541 aatatcctat gtacaatgat gtaatcgaaa ggatatcttt atgtgagttt atcggcgata
     6601 tcttctatat ctaagctagc aagttgttgc atcgtagcta aggatttatc taaaaatact
     6661 atgaaattgg acgtcatatt ttttgaggat aaaaataaaa gatccgcagt tttaggttta
     6721 cgaagagaca aaagcggagt atttaaacca gttactctac attttacaag cgctaagaaa
     6781 tatgctaaag ttcgtaaaac agatgtgaaa gaaatgaaat ggctataaag attgtccgca
     6841 ggcttaagct tgcgtttttt gttgtttaac gaaattaaaa aacaagctct ctcatcggaa
     6901 atgtttttat aggaatgaag gaagatgatg ctatgtttaa aaagagaatc gaaaagggca
     6961 aaagtagtat ctttatctat tttttggagt agaagcttat gatatagaaa tttgtaataa
     7021 aaatgtaata taaaacattt gacaaat
//
LOCUS       DAVHEX010000081         6828 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVHEX010000081 DAVHEX010000000
VERSION     DAVHEX010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6828
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.082"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            67..6486
                     /locus_tag="VVL45_001965"
     CDS             67..6486
                     /locus_tag="VVL45_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU4188092.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALSISDKEKV
                     AENKEKHEDIHSAMETSKDTEEKKATVIEEKEVVSKNPVIDTKTSNEEARIKEDSNQS
                     QGDNAHSSANKGTENPKKEDRLVYIAEFKDKESGEKAIKELSSLKNTKVLYTYDRIFN
                     GSAIETTPDNLDKIKQIEGISSVERAQKVQPMMNHARKEIGVEEAIDYLKSINAPFGK
                     NFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKIPHAFN
                     YYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQIFSYK
                     MYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRALRKAGI
                     PMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQTVEFDK
                     VNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDLRGKIA
                     VMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGTKSQVF
                     SISGDDGVKLWNMINPNKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVGDEKEI
                     DFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVINGKSTY
                     GYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQNTARP
                     MMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGSISLKE
                     IKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPDGKQIVPE
                     IHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESVEEMEA
                     LNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDGKPKIPG
                     TLNKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNQGINAPSSSGSKIAN
                     IYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVISREHFI
                     RGILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATKIRGQF
                     EPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLITKDTYH
                     KVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYAGEGQG
                     RNRKLDKDGNTIYEIKGAGDLRGKIIEVIALDGSSNFTKIHRIKFADHADEKGMISYY
                     LVDPDQDSSKYQKLGEIAESKFKNLGNGKEGSLKKDTTEVEHHHQENEESIKEKSSFT
                     IDRNISTIRDFENKDLKKLIKKKFREVEDFTSETGKRKQEYDYKYDDKGNIIAYDDES
                     ALEYETEKFDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNNYKFIEIKATK
                     YDSHSKTMTFDLYANINDIVDGLAFAGDMRFFVKDDDRIKAETKIRMPEKNKETKAEY
                     PYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKGYALKV
                     TTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRALSETTIYADSRNVEDGRSTQSVLM
                     SALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDKFNVEA
                     IKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNWELRVN
                     ESVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMDGNVYL
                     QTGYSDLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKRNNGFD
                     GEIQEQHIYINGKEYTSFNDIKQIIDKTLNIKIVVKDFARNTTVKEFILNKDTGEVSE
                     LKPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKKDAGYV
                     INLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDLQREEH
                     SQKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIVGIFLG
                     LKKKNQD"
BASE COUNT         2696 a          925 c         1327 g         1880 t
ORIGIN      
        1 aacaaaggct agcaaagtta aagtttatta tctattggaa gttatataaa tatgtaagga
       61 attaaaatga aaaaaagtac agtattgtca ttaaccacag ctgcagttat tttagcagcc
      121 tatgccccta atgaggtagt cttagcagac acatctagct ctgaagatgc tttaagcatc
      181 tctgataaag aaaaagtagc agaaaataaa gagaaacatg aagatatcca tagtgctatg
      241 gaaacttcaa aggatactga agaaaagaaa gcaacagtta ttgaggaaaa agaagttgtt
      301 agtaaaaatc ctgtgataga cactaaaact agcaatgaag aagcaagaat caaagaagat
      361 tccaatcaat cccaaggaga taatgcacac tcgtctgcaa ataaaggcac agaaaatccc
      421 aaaaaagaag atagacttgt ctatattgct gaatttaaag ataaagaatc tggagaaaaa
      481 gcaatcaagg aactatccag tcttaagaat acaaaagttt tatatactta tgatagaatt
      541 tttaacggta gtgccataga aacaactcca gataacttgg acaaaattaa acaaatagaa
      601 ggtatttcat cggttgaaag ggcacaaaaa gtccaaccca tgatgaatca tgccagaaag
      661 gaaattggag ttgaggaagc tattgattac ctaaagtcta tcaatgctcc atttgggaaa
      721 aattttgatg gtagaggtat ggtcatttca aatatcgata ctggaacaga ttatagacat
      781 aaggctatga gaatcgatga tgatgccaaa gcctcaatga gatttaaaaa agaagactta
      841 aaaggaactg ataaaaatta ttggttgagt gataaaatcc ctcatgcgtt caattattat
      901 aatggtggca aaatcactgt agaaaaatat gatgatggaa gggattattt tgacccacat
      961 gggatgcata ttgcagggat tcttgctgga aatgatactg aacaagatat caaaaacttt
     1021 aacggcatag atggaattgc acctaatgca caaattttct cttacaaaat gtattctgac
     1081 gcaggatctg ggtttgcggg tgatgaaaca atgtttcatg ctattgaaga ttctatcaaa
     1141 cacaacgttg atgttgtttc ggtatcatct ggttttacag gaacaggtct tgtaggtgag
     1201 aaatattggc aagctattcg ggcattaaga aaagcaggca ttccaatggt tgtcgctacg
     1261 ggtaactatg cgacttctgc ttcaagttct tcatgggatt tagtagcaaa taatcatctg
     1321 aaaatgaccg acactggaaa tgtaacacga actgcagcac atgaagatgc tatagcggtc
     1381 gcttctgcta aaaatcaaac agttgagttt gataaagtta acataggtgg agaaagtttt
     1441 aaatacagaa atataggggc ctttttcgat aagaataaaa tcacaacaaa tgaagatgga
     1501 acaaaagctc ctagtaaatt aaaatttgta tatataggca aggggcaaga ccaagatttg
     1561 ataggtttgg atcttagggg caaaattgca gtaatggata gaatttatac aaaggattta
     1621 aaaaatgctt ttaaaaaagc tatggataag ggtgcacgcg ccattatggt tgtaaatact
     1681 gtaaattact acaatagaga taattggaca gagcttccag ctatgggata tgaagcggat
     1741 gaaggtacta aaagtcaagt gttttcaatt tcaggagatg atggtgtaaa gctatggaac
     1801 atgattaatc ctaataaaaa aactgaagtc aaaagaaata ataaagaaga ttttaaagat
     1861 aaattggagc aatactatcc aattgatatg gaaagtttta attccaacaa accgaatgta
     1921 ggtgacgaaa aagagattga ctttaagttt gcacctgaca cagacaaaga actctataaa
     1981 gaagatatca tcgttccagc aggatctaca tcttgggggc caagaataga tttactttta
     2041 aaacccgatg tttcagcacc tggtaaaaat attaaatcca cgcttaatgt tattaatggc
     2101 aaatcaactt atggctatat gtcaggaact agtatggcga ctccaatcgt ggcagcttct
     2161 actgttttga ttagaccgaa attaaaggaa atgcttgaaa gacctgtatt gaaaaatctt
     2221 aagggagatg acaaaataga ccttacaagt cttacaaaaa ttgccctaca aaatactgcg
     2281 cgacctatga tggatgcaac ttcttggaaa gaaaaaagtc aatactttgc atcacctaga
     2341 caacagggag caggcctaat taatgtggcc aatgctttga gaaatgaagt tgtagcaact
     2401 ttcaaaaaca ctgattctaa aggtttggta aactcatatg gttccatttc tcttaaagaa
     2461 ataaaaggtg ataaaaaata ctttacaatc aagcttcaca atacatcaaa cagacctttg
     2521 acttttaaag tttcagcatc agcgataact acagattctt taactgacag attaaaactt
     2581 gatgaaacat ataaagatga aaaatctcca gatggtaagc aaattgttcc agaaattcac
     2641 ccagaaaaag tcaaaggagc aaatatcaca tttgagcatg atactttcac tataggcgca
     2701 aattctagct ttgatttgaa tgcggttata aatgttggag aggccaaaaa caaaaataaa
     2761 tttgtagaat catttattca ttttgagtca gtggaagaaa tggaagctct aaactccaac
     2821 gggaagaaaa taaacttcca accttctttg tcgatgcctc taatgggatt tgctgggaat
     2881 tggaaccacg aaccaatcct tgataaatgg gcttgggaag aagggtcaag atcaaaaaca
     2941 ctgggaggtt atgatgatga tggtaaaccg aaaattccag gaaccttaaa taagggaatt
     3001 ggtggagaac atggtataga taaatttaat ccagcaggag ttatacaaaa tagaaaagat
     3061 aaaaatacaa catccctgga tcaaaatcca gaattatttg ctttcaataa ccaagggatc
     3121 aacgctccat catcaagtgg ttctaagatt gctaacattt atcctttaga ttcaaatgga
     3181 aatcctcaag atgctcaact tgaaagagga ttaacacctt ctccacttgt attaagaagt
     3241 gcagaagaag gattgatttc aatagtaaat acaaataaag agggagaaaa tcaaagagac
     3301 ttaaaagtca tttcgagaga acactttatt agaggaattt taaattctaa aagcaatgat
     3361 gcaaagggaa tcaaatcatc taaactaaaa gtttggggtg acttgaagtg ggatggactc
     3421 atctataatc ctagaggtag agaagaaaat gcaccagaaa gtaaggataa tcaagatcct
     3481 gctactaaga taagaggtca atttgaaccg attgcggaag gtcaatattt ctataaattt
     3541 aaatatagat taactaaaga ttacccatgg caggtttcct atattcctgt aaaaattgat
     3601 aacaccgccc ctaagattgt ttcggttgat ttttcaaatc ctgaaaaaat taagttgatt
     3661 acaaaggata cttatcataa ggtaaaagat cagtataaga atgaaacgct atttgcgaga
     3721 gatcaaaaag aacatcctga aaaatttgac gagattgcga acgaagtttg gtatgctggc
     3781 gccgctcttg ttaatgaaga tggagaggtt gaaaaaaatc ttgaagtaac ttacgcaggt
     3841 gagggtcaag gaagaaatag aaaacttgat aaagacggaa ataccattta tgaaattaaa
     3901 ggtgcaggag atttaagagg aaaaatcatt gaagtcattg cattagatgg ttctagcaat
     3961 ttcacaaaga ttcatagaat taaatttgct gatcatgctg atgaaaaggg gatgatttct
     4021 tattacctag tagatcctga tcaagattca tcaaaatatc aaaagcttgg cgagattgca
     4081 gaatctaaat ttaaaaattt aggaaatgga aaagagggta gtctaaaaaa agatacaact
     4141 gaggtagaac atcatcatca agaaaatgaa gagtctatta aagaaaaatc tagttttact
     4201 attgatagaa atatttcaac aattagagac tttgaaaata aagacttaaa gaaactcatt
     4261 aaaaagaaat ttagagaagt tgaggacttt acaagtgaaa ctggtaagag gaaacaagaa
     4321 tacgattata aatacgatga taagggaaat atcattgctt atgacgatga aagtgctcta
     4381 gaatatgaaa ctgaaaaatt tgacgaaata aaatcaaaaa tttatggtgt tctaagccca
     4441 tctaaagatg gacactttga aattcttgga aagataagca atgtttctaa aaatgccaag
     4501 gtatattatg gaaataacta taaatttata gaaatcaaag cgactaagta tgactcacat
     4561 tcaaaaacga tgacatttga tttatacgct aatattaatg atattgtgga tggattagct
     4621 tttgctggag atatgcggtt ttttgttaaa gatgatgatc gaataaaagc tgaaactaaa
     4681 attagaatgc ctgaaaaaaa taaggaaact aaagcagaat atccctatgt atcaagttat
     4741 gggaatgtaa tcgaattagg agaaggagat ctttcaaaaa acaaaccaga caatttaact
     4801 aaaatggaat ctggtaaaat ctattctgat tcagaaaaac aacaatatct gttaaaggat
     4861 aatatcattc taagaaaagg ctatgcacta aaagtgacta cctataatcc tggaaaaacg
     4921 gatatgttag aaggaaatgg agtctatagc aaggaagata tagcaaaaat acaaaaggcc
     4981 aatcctaatc taagagccct ttcagaaaca acaatttatg ctgatagtag aaatgttgaa
     5041 gatggaagaa gtacccaatc tgtattaatg tcggctttgg acggctttaa cattataagg
     5101 tatcaagtgt ttacatttaa aatgaacgat aaaggggaag ctatcgataa agacggaaat
     5161 cttgtgacag attcttctaa acttgtatta tttggtaagg atgataaaga atacactgga
     5221 gaggataagt tcaatgtaga agctataaaa gaagatggct ccatgttatt tattgatacc
     5281 aaaccagtaa acctttcaat ggataagaac tactttaatc catctaaatc taataaaatt
     5341 tatgtacgaa atccagaatt ttatttaaga ggtaagattt ctgataaggg tggttttaac
     5401 tgggaattga gagttaatga atcggttgta gataattatt taatctacgg agatttacac
     5461 attgataaca ctagagattt taatattaag ctgaatgtta aagacggtga catcatggac
     5521 tggggaatga aagactataa agcaaacgga tttccagata aggtaacaga tatggatgga
     5581 aatgtttatc ttcaaactgg ctatagcgat ttgaatgcta aagcagttgg agtccactat
     5641 cagtttttat atgataatgt taaacccgaa gtaaacattg atcctaaggg aaatactagt
     5701 atcgaatatg ctgatggaaa atctgtagtc tttaacatca atgataaaag aaataatgga
     5761 ttcgatggtg agattcaaga acaacatatt tatataaatg gaaaagaata tacatcattt
     5821 aatgatatta aacaaataat agacaagaca ctaaacatta agattgttgt aaaagatttt
     5881 gcaagaaata caaccgtaaa agaattcatt ttaaataaag atacgggaga ggtaagtgaa
     5941 ttaaaacctc atagggtaac tgtgaccatt caaaatggaa aagaaatgag ttcaacgata
     6001 gtgtcggaag aagattttat tttacctgtt tataagggtg aattagaaaa aggataccaa
     6061 tttgatggtt gggaaatttc tggtttcgaa ggtaaaaaag acgctggcta tgttattaat
     6121 ctatcaaaag atacctttat aaaacctgta ttcaagaaaa tagaggagaa aaaggaggaa
     6181 gaaaataaac ctacttttga tgtatcgaaa aagaaagata acccacaagt aaaccatagt
     6241 caattaaatg aaagtcacag aaaagaggat ttacaaagag aagagcattc acaaaaatct
     6301 gattcaacta aggatgttac agctacagtt cttgataaaa acaatatcag tagtaaatca
     6361 actactaaca atcctaataa gttgccaaaa actggaacag caagcggagc ccagacacta
     6421 ttagctgccg gaataatgtt tatagtagga atttttcttg gattgaagaa aaaaaatcaa
     6481 gattaagata aaagctatag aaaaaaatgg tttatgtact gagattagat agtgaggtga
     6541 tgacatagtt ttgtgaaaat agccatttat aactcaatta tttagtttac tttactttac
     6601 tagtgatact atttggagtt attaatggac ttagtttata taactaatga attgattgaa
     6661 agggttagta ttgacaatat tggtcatatt gactagaaaa tagagtctat caaaatttaa
     6721 aggctaatag aggtgatgag acaatttcgg ctctttgtca actgtagtgg gttgaagtca
     6781 gctaagctcg agaaaggaca aattttgtcc tttctttttt gatattca
//
LOCUS       DAVHEX010000082         6716 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVHEX010000082 DAVHEX010000000
VERSION     DAVHEX010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6716
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.083"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            159..434
                     /locus_tag="VVL45_001966"
                     /pseudo
     CDS             159..434
                     /locus_tag="VVL45_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            601..801
                     /locus_tag="VVL45_001967"
     CDS             601..801
                     /locus_tag="VVL45_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188093.1"
                     /translation="MVASASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            843..1247
                     /locus_tag="VVL45_001968"
     CDS             843..1247
                     /locus_tag="VVL45_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188094.1"
                     /translation="MDSLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETNSVPA
                     GVIVVSLLALLGVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKAQNLL
                     QETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            1478..2185
                     /gene="ccdA2"
                     /locus_tag="VVL45_001969"
     CDS             1478..2185
                     /gene="ccdA2"
                     /locus_tag="VVL45_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEU4188095.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYGMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            2196..2753
                     /gene="sdbB"
                     /locus_tag="VVL45_001970"
     CDS             2196..2753
                     /gene="sdbB"
                     /locus_tag="VVL45_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HEU4188096.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIA
                     VGKDAPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            3127..4518
                     /locus_tag="VVL45_001971"
     CDS             3127..4518
                     /locus_tag="VVL45_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU4188097.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIAFPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKIPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            4661..5578
                     /gene="adcAII"
                     /locus_tag="VVL45_001972"
     CDS             4661..5578
                     /gene="adcAII"
                     /locus_tag="VVL45_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HEU4188098.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            5586..>6716
                     /locus_tag="VVL45_001973"
     CDS             5586..>6716
                     /locus_tag="VVL45_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEU4188099.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVSYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVDGKYYVYLKDAAHADNIRTKEEIKRQKQEH
                     SHNHGGGSNDQAVVAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKNE
                     LSASELAAAEAYWNGKQGSRPSSSSSHNANPAQPRLSENHNLTVTPTYHQNQGENISS
                     LLRELYAKPLSERHVESDGLIFDPAQITSRTARGVAVPHGNHYHFIPYEQMSELEKRI
                     ARIIPLRYRSNHWVPDSRPEQPSPQSTPEPSPSPQPAPNPQPA"
BASE COUNT         2055 a         1229 c         1436 g         1996 t
ORIGIN      
        1 accaagggag gagtctgccc ttttgaggaa atctagcgag gaaaaacgat actggaacag
       61 cagaaagtaa aactgacctc atgaggagga agaaagtggc tcatgaggtc aggggttttg
      121 taagttacat ctagttgaga gaggtatgaa tgatttgggt taatagttta aagcacctag
      181 cacctgttta gattgacgaa acaggattcg atacttattt ttatcgagaa tatggtcgct
      241 cattaaaagg tcagttaata agaggcaaag tatctggaag aagatatcag aggatttctt
      301 tggttgcagg tctaacaaat ggtgaattaa tcgctccaat gacttacgaa gagacgatga
      361 cgagcgactt ttttgaagct tggtttcaga agtttctctt accaacatta accacaccat
      421 cggttattat agtaaaatga aataagaata ggacaaatcg atcaggacag taaaatcgaa
      481 ttctaacaat gttttagaag tccatatgta ctattctagt ttcaatatac cataactgtt
      541 cctagtcaac ctgcaagccc tgtcccagct ccaagctcag ctccgactca agatcctaga
      601 gtggtagcaa gcgcttcggc tagctcaacg agcacacaag ctcaagagca agttgacaag
      661 tctgaacttc gtgccttgag tcaagagtta gaccaacgct tgaaagcttt ggcaacagta
      721 tctgatccga aaatcgatgc aaccaaggct gtcctcctag atgctcaaaa agctccggaa
      781 gatagtgcct tgacagagta aggtctgcat actgcagtag agtctgtgaa ggctgggctg
      841 ccttggactc tctcaaagat gtgaaagcaa atgctagcga cagcaagcct gcacaggaca
      901 agaaggatgc aaaacaagga acggaagata gtaaggattc agataagatg actgaaacaa
      961 actcagttcc ggcaggagtg attgtggtca gtctacttgc cctcctaggc gtgattgcct
     1021 tctggctgat tcgccgtaag aaagagtcag aaatccagca attaagcacg gaattgatca
     1081 aggttctagg acagctagat gcagaaaaag cggataaaaa agtccttgcc aaagcccaaa
     1141 accttctcca agaaaccctt gatttcgtga aagaagaaaa tggctcagca gagacagaaa
     1201 ctaaactagt agaggagctt aaagcaatcc ttgacaaact caagtaaaac atgatttatg
     1261 aaatgtcaga cctaaaagac attttcgttc acaaaagcag cgatgagtaa tccttgtcgt
     1321 tgctttttgg ctattttggg ggataagatc cgccttgact agcaaaagaa aagattggtc
     1381 caatagtgcc cgatacgatt ttgcgtagca gaaagcttac aaataaaatt gaatatagta
     1441 aaataggatt agaattatta agaaagttgg ttctttattg gaaacgatag tatttttaat
     1501 ctctgttttt ctagcaggtg ttttatcctt tttttctcct tgtatttttc ctcttctgcc
     1561 agtctatgct gggattttat tggatgatca agaaagtgca aaaagctttt ctttgtttgg
     1621 gagaaaggtt ctctggtcag gcttgattcg aacactttgc tttatcgctg gtatctctct
     1681 cattttcttt attctaggct ttggtgctgg ttactttggt catattctct atgcaaattg
     1741 gtttcgatat ggcatgggag ctattattat cattttgggt cttcaccaga tggaaatttt
     1801 tcatttgaag aaattagaag ttcaaaaaag ttttaccttt aaaaaatcag attctaatcg
     1861 ttattggtca gcttttttac ttggtattac ctttagcttt ggttggacac cttgtattgg
     1921 tccagtttta agttctgttt tagcacttgc ggcttctgga ggcaatggcg cttggcaagg
     1981 agcgatttat actctcattt acactctggg catggccctt cctttcttgg tattggcact
     2041 agcttcaggt ctagtcatgc catattttag taaaatcaag cgtcatatga tgctactaaa
     2101 gaaaattggt ggtttcctca ttgttttaat gggaattttg ttactattag gacaagtaaa
     2161 tgttctagct ggaatttttg aataaaggag gacaaatgaa aaaagtaatg tttgctggct
     2221 taagtctctt gtcattagtt gtattgatgg cctgtggtga ggaagaaact aaaaagactc
     2281 aagcagcaca acagccaaaa caacaaacga ctgtacaaca aattgctgtt ggaaaagatg
     2341 ctccagactt cacattgcaa tccatggatg gcaaagaagt taagttatct gattttaagg
     2401 gtaaaaaggt ttacttgaag ttttgggctt catggtgtgg tccatgcaag aaaagtatgc
     2461 cagagttgat ggaactagcg gcgaaaccag atcgtgattt cgaaattctt actgtcattg
     2521 caccaggaat tcaaggtgaa aaaactgttg agcaattccc acaatggttc caggaacaag
     2581 gatataagga tatcccagtt ctttatgata ccaaagcaac caccttccaa gcttatcaaa
     2641 ttcgaagcat tcctacagaa tatttaattg atagccaagg aaagattgga aagattcaat
     2701 ttggtgctat cagtaatgcg gatgcagaag cagcatttaa agaaatgaac tagaatcgat
     2761 aaaatctgat tacaagatgt agtcagattt tggtaacttc taaaactaac ttccagtttc
     2821 ccacaaccta gcttttagta tgagaaaaac gcgtgacatc agtggaaatc cgtcttagac
     2881 taacttccac tggttttctt tttcttgata tataagggtt caaaagcgaa aagactcaga
     2941 aaaagtgcac gacaaatagc cctaaaacag agtcgtctta gagtaacttc cagttttggg
     3001 agagagatgg aagttacttt gagaagttac ggtagtcaga tttttttaga aaaacatttt
     3061 ataaatattc acatatctcc tatatttatg gtaaaataga attatcagtt tattttggag
     3121 tcaaagatga atatatttag aacaaagaat gttagtttag ataaaacaga gatgcatagg
     3181 catttgaagt tatgggattt gattttgctg ggtatcggag ccatggtagg gacaggcgtc
     3241 tttacaatca caggtactgc agctgcaaca cttgctggcc cagccctagt gatttcaatc
     3301 gttatttctg ccttgtgtgt gggattatca gccctctttt ttgcagaatt tgcctcgcga
     3361 gtacccgcta caggaggtgc ctatagttac ctctatgcta tcttaggaga attccctgcc
     3421 tggttggctg gttggttaac catgatggag ttcatgacag ccatatcagg cgtagcttcg
     3481 ggttgggcag cttattttaa aggcttgctc tctcaatacg ggatagcctt tcctcaggct
     3541 ttaaatggta cctttaatcc tcaagcaggg acatttgttg atttattgcc tattcttgtc
     3601 ttggtcttgg tgacttcgct tgttttacta aatgccaagg cagccttacg ctttaattcg
     3661 attctagtta ttttgaaatt ttccgcttta gctctctttg ttttagtagg aatttggaat
     3721 attaagtttg ataattggag caattttgct ccttacggtt ttggacaaat ctatggagct
     3781 agcactggta ttatggctgg tgcgtccttg atgttcttcg gttttttggg atttgaatcc
     3841 atctctatgg ctgtagatga agtcaagact cctcaaaaaa atattcctag agggattgtc
     3901 ttatcgcttt ctatcgtaac cattctctat gccttggtga cccttgtttt gactggtgtg
     3961 gttcactata gtcatttaaa tgtcgatgat gccgttgcct ttgcccttcg tagtgttggg
     4021 attagttggg cagccaacta tgtgtcatta gtggctatct tgaccttgat tacagtttgt
     4081 atttcgatga cctatgccct atcgcgtatg atttacagtt tagcacgtga cggcttagtg
     4141 cctgctgcct ttaaggaact gacgaagact agcaagatac caaagaatgc tactatcttg
     4201 acaggtctag cttcagcagt agcagcagga atgttcccac tagccagtat tgcagccttt
     4261 ttaaatattt gtaccttagc ctacttgatc atgttggctt atggtctgat tcgcttacgg
     4321 aaagaaaaag gaatgcccaa agctggggaa tttaaaacac cattggtacc tctgttacca
     4381 attttatcga tcatcatttg tttatccttc atgttacagt ataatatgaa tacctggatt
     4441 gctttcctag ttgcgttgtt agtaggaagt attatttact tcacttatgg ctataagcat
     4501 tctaccatag aagaataaag tgaaagtcta ttgagttgtt gacactcagt agatttttat
     4561 atgtgaaaga aatttcaaat tttttctaaa aatatcttga cagattaaat tttcaggagt
     4621 agaatattta ctagttaatt aaatggttaa ggagttgttc atgaagaaac aaaatttatt
     4681 tttagtcctg ttaagtgtct ttcttttgtg cttgggggct tgtggtcaaa aggaaagtca
     4741 gacaggaaag gggatgaaaa ttgtgaccag tttttatcct atctacgcta tggttaagga
     4801 agtatctggt gacttgaatg atgttcggat gattcagtca agtagtggta ttcactcctt
     4861 tgaaccttcg gcaaatgata tcgcagccat ctatgatgca gatgtctttg tttaccattc
     4921 tcatacactc gaatcttggg caggaagtct ggatccaaat ctaaaaaaat ccaaagtgaa
     4981 ggtcttagag gcttctgagg gaatgacctt ggaacgtgtc cctggactag aggatgtgga
     5041 agcaggggat ggagttgatg aaaaaacgct ctatgaccct cacacatggc tagatcctga
     5101 aaaagctgga gaagaagccc aaattatcgc tgataaactt tcagaggtgg atagtgagca
     5161 taaagagact tatcaaaaaa atgcgcaagc ctttatcaaa aaagctcagg aattgactaa
     5221 gaaattccaa ccaaaatttg aaaaagcgac tcagaaaaca tttgtaacac aacatacagc
     5281 cttttcttat ctagcgaaga gatttgggct taatcaactt ggtattgcag gtatctctcc
     5341 tgaacaagaa ccaagtccac gacaactaac agaaattcag gaatttgtta agacctataa
     5401 ggttaaaacg atttttacag aaagtaacgc ttcttcaaaa gtagctgaaa ctcttgtcaa
     5461 atcaacaggt gtgggtctta aaactctgaa tcctttagag tcagacccac aaaatgacaa
     5521 gacctattta gaaaatcttg aagaaaatat gagtattcta gcagaagaat taaagtgagg
     5581 aaagaatgaa aattaataaa aaatatctag caggttcagt ggcagtcctt gccctaagtg
     5641 tttgttccta tgaacttggt cgtcaccaag ctggtcaggt taagaaagag tctaatcgag
     5701 tttcttatat agatggtgat caggctggtc aaaaggcaga aaacttgaca ccagatgaag
     5761 tcagtaagag ggaggggatc aacgccgaac aaatcgtcat caagattacg gatcaaggtt
     5821 atgtgacctc tcatggagac cattatcatt actataatgg caaggtccct tatgatgcca
     5881 tcatcagtga agagctcctc atgaaagatc cgaattatca gttgaaggat tcagacattg
     5941 tcaatgaaat caagggtggt tatgttatca aggtagatgg aaaatactat gtttacctta
     6001 aggatgcagc tcatgcggat aatattcgga caaaagaaga gattaaacgt cagaagcagg
     6061 aacacagtca taatcacggg ggtggttcta acgatcaagc agtagttgca gccagagccc
     6121 aaggacgcta tacaacggat gatggttata tcttcaatgc atctgatatc attgaggaca
     6181 cgggtgatgc ttatatcgtt cctcacggcg accattacca ttacattcct aagaatgagt
     6241 tatcagctag cgagttagct gctgcagaag cctattggaa tgggaagcag ggatctcgtc
     6301 cttcttcaag ttctagtcat aatgcaaatc cagctcaacc aagattgtca gagaaccaca
     6361 atctgactgt cactccaact tatcatcaaa atcaagggga aaacatttca agccttttac
     6421 gtgaattgta tgctaaaccc ttatcagaac gccatgtgga atctgatggc cttattttcg
     6481 acccagcgca aatcacaagt cgaaccgcca gaggtgtagc tgtccctcat ggtaaccatt
     6541 accactttat cccttatgaa caaatgtctg aattggaaaa acgaattgct cgtattattc
     6601 cccttcgtta tcgttcaaac cattgggtac cagattcaag accagaacaa ccaagtccac
     6661 aatcgactcc ggaacctagt ccaagtccgc aacctgcacc aaatcctcaa ccagct
//
LOCUS       DAVHEX010000083         6682 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVHEX010000083 DAVHEX010000000
VERSION     DAVHEX010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6682
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.084"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            352..3396
                     /locus_tag="VVL45_001974"
     CDS             352..3396
                     /locus_tag="VVL45_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HEU4188100.1"
                     /translation="MSQAEFEVRAGSIRQEILNATKDKADKTLVVSEAGKLREEFSKM
                     KVGGRNLWIKSKTVGAVIEKLPENHVTGQKECYRLENNSTLTFNIEPDFSSRLYQKVT
                     FSAWVKYENVVQGRNFWNVFNCFKHYLFRKNSETGVQSGPDYATLGMYKGSADWKYIT
                     FTYDYSEKTNFDQLKTSLRFNLEGATSGTAWVTGIKVEIGSVATDWSPAPEDADGLIT
                     EAKATFERTAQGLRTDLSAIQEYVNKDGQRQEALQRYTREESTRQAIAVRELVNRDFV
                     GKATYQEDVKGINQRIEAVKTSANKDIASQIASYRQSVDGKFTDISSQITTYKQDVGG
                     QISGLSNRLTSSEQGTTTQISNLSNRINSNKQGADNQISNLKTQVATNKDNAERQMGR
                     ISDQVSANKANADSQFANVTNQLVRKVETTDFQRVKETSKLYERILGNTENGIADKVA
                     RMALTNQLFQVEVGKVAKGGRNYIRNGQFKNGSKNWLEYQSVNFGLNFNYQHSQNPNN
                     RNRPGLHFYHDSQDVANFFGIQQSFAFDGVRGEKVSVSLLVSKDGGDSNSGLKVALHY
                     IKNKNIIGQEWQNIPSPQITSKYKRFTFTFTLSDDVENLNLMLFGEKGKTINLYVTDV
                     QLERGSVATDYKEAPEDTDEAIRSVQSQLTGSWAVQNINSAGDIISGINLGANGHNRF
                     VGKLTHITGETLIDRAVIKSAMVDKLKTANFEAGSVTTTILDAEAVTAEKLKVDNALI
                     RKLTANDAFIDQLISKRIFSTKVESVISSSTFLEAYQGRIGGFTLGQFDQGGGRWISG
                     VNQFSVGMGNGAGYGVRTAFWANWGNNWNYAGPKAWNVNTDGKMYCRNEVGFYDQVDF
                     SNSSRANFYGNTTFSRSPVFSNGIELGSKDVLGDGWNPKGGRNAVVWWNQVGSGSVKY
                     WMEQKSDRRLKENITDTAVKALDKINRLRMVAFDFIENKKHEEIGLIAQEAETIVPKI
                     VSRDPENPDGYLHIDYTALVPYLIKAIQELNQKIEKMEKTIA"
     gene            3490..3693
                     /locus_tag="VVL45_001975"
     CDS             3490..3693
                     /locus_tag="VVL45_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188101.1"
                     /translation="MNTEQLNQALQMTISEMSTTSTNSMITSNILSIQLNEQREENQR
                     LQARVDELEALLDEQTKPADKGE"
     gene            3696..4046
                     /locus_tag="VVL45_001976"
     CDS             3696..4046
                     /locus_tag="VVL45_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188102.1"
                     /translation="MAETIQNTDNLLDLTKITEPFDLASALRYMKENGEFIRCKNVSD
                     DFYMYRDVQKRPVIVNGRRQFKDVETVWAFNQWGGTIATINVAVLLNHEFYIMKFDAE
                     GNPDWTVPTVEPKE"
     gene            4055..4471
                     /locus_tag="VVL45_001977"
     CDS             4055..4471
                     /locus_tag="VVL45_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEU4188103.1"
                     /translation="MQIEFFNFLRSVVQTEDGLVLYALALIVSMEIIDFVTGTIAAII
                     NPDIEYKSKIGINGLFRKISGVLLLMILIPASVLLPEKTGFAFLYSIYLGYIAFTFQS
                     LIENYRKLKGNVTLFQPIVKVFQRLLEKDDDTKKGE"
     gene            4475..4807
                     /locus_tag="VVL45_001978"
     CDS             4475..4807
                     /locus_tag="VVL45_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEU4188104.1"
                     /translation="MQQITEIITNGAISILVILAGIAVKAVKDYLVQKGGEKTIKIVE
                     ILAKNAVNAVEQVAAETGYKGDEKLAQARAKVRAELTKYNISMTDKNLDTFVESAVKQ
                     MNDVWKGR"
     gene            4811..5767
                     /locus_tag="VVL45_001979"
     CDS             4811..5767
                     /locus_tag="VVL45_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU4188105.1"
                     /translation="MDIDRNRLRTGLPQVGVQPYRQVHAHSTGNRNSTVQNEADYHWR
                     KDPELGFFSHVVGNGRVMQVGPVNNGSWDVGGGWNAESYAAVELIESHSTKEEFMADY
                     RLYIELLRNLADEAGLPKTLDTDDLAGIKTHEYCTNNQPNNHSDHVDPYPYLTKWGIS
                     REQFKQDIENGLSAATGWQKNGTGYWYVHSDGSYPKDKFEKINGTWYYFDGSGYMLSD
                     RWKKHTDGNWYYFDQSGEMATGWKKIAEKWYYFDVEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            complement(6038..6217)
                     /locus_tag="VVL45_001980"
     CDS             complement(6038..6217)
                     /locus_tag="VVL45_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188106.1"
                     /translation="MREVIQELLDSSMSTSAISQGAGVPWTTVSDLRKGKTSMDKMAL
                     LTAEKLYEFATTDKQ"
     gene            complement(6359..6508)
                     /locus_tag="VVL45_001981"
     CDS             complement(6359..6508)
                     /locus_tag="VVL45_001981"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188107.1"
                     /translation="MNKEDWLRLLEKAIDNIPETVTAIASLVTAITVARQNKKRKPKS
                     RQRKR"
BASE COUNT         2153 a         1237 c         1502 g         1790 t
ORIGIN      
        1 cgatatgttg tccatattcc aaaaggttgg acaaacgaat ccaagcagac cacaaatgaa
       61 tggttgttca atttcaacca ggaaggaacc atttggattt ggatgccgaa gtttgaaata
      121 agcgatgtag atacttctta ttcagaagct cctgaagata tagaaggtca gatttcaaca
      181 gtagaatcga cctttaaaca acgagccaac tcgctcgacg ctggtgtgag aagcttgact
      241 gaagggctta gaaccaaagt ggatatcagc tcactcaacg tgactgctga aaatatccgg
      301 caatctgtga agagtcttga gacagacacg cagaacaagc taaatcagaa gttgagtcag
      361 gctgaatttg aggtgcgagc tggctctatc cgtcaggaaa tcctgaacgc aaccaaggat
      421 aaagcagata agactttagt tgtatctgaa gctgggaaat tgcgtgaaga attttcaaaa
      481 atgaaggtgg gaggacggaa tctatggata aaatccaaga cggttggagc tgtaattgaa
      541 aaattacctg aaaaccacgt cacaggtcaa aaagaatgct ataggctaga gaacaactct
      601 actttaacgt tcaacattga accagatttc agctcaaggt tgtatcaaaa agttactttt
      661 agcgcttggg tcaagtacga aaatgtagtc caaggtcgaa atttttggaa tgtatttaat
      721 tgcttcaaac attatctttt tagaaaaaat agtgagaccg gagtacagag tggtccagat
      781 tatgctacgc ttggtatgta taaaggttcg gcagattgga aatatattac attcacttat
      841 gactactctg aaaaaacaaa ttttgatcaa ttgaagacat cattgcgatt caatcttgaa
      901 ggtgctacaa gcggtacagc ttgggtaaca ggaatcaagg ttgaaatcgg tagtgtggcg
      961 acggactgga gtcctgcgcc tgaggacgct gatggtctca tcactgaagc taaggctacc
     1021 tttgagcgga cagctcaggg cttgcgaacc gatttatcag ctattcagga atatgtaaat
     1081 aaagacggtc agcgacagga agccctacag cgctatactc gtgaggagag cacgagacaa
     1141 gcgatagcag tccgtgagct ggtcaatcgt gatttcgttg gtaaggctac ttatcaagaa
     1201 gatgttaagg gtatcaatca aaggattgaa gctgttaaaa ctagtgcgaa taaagacatc
     1261 gctagtcaaa tcgctagcta tcgtcaatct gtagatggta agttcacgga tatttcaagt
     1321 cagataacta cttataagca agatgtgggc ggtcaaatca gtggcctttc aaatagactt
     1381 acaagcagtg agcaaggaac cactactcag atttcaaatc tttcaaatcg gataaacagt
     1441 aataaacaag gcgcagataa tcagatttca aatttaaaga ctcaggtcgc tacaaacaag
     1501 gataatgctg aacgacaaat gggtagaata tctgatcagg tttctgcaaa caaagcgaat
     1561 gctgatagtc aatttgcgaa tgtgaccaat caactagtgc gaaaagtaga gactactgac
     1621 ttccagcgtg ttaaggaaac cagtaaactt tacgagcgga ttttgggcaa tactgaaaat
     1681 ggaattgcgg ataaggttgc tcgcatggct ctgaccaatc aactgtttca ggttgaggtt
     1741 gggaaggttg ccaaaggtgg tcgaaattat atcagaaatg gtcaatttaa gaacggttcg
     1801 aaaaactggc ttgaatatca atctgttaat tttggtttga atttcaacta tcaacactct
     1861 caaaatccta ataatcgaaa tcgtccagga ctacacttct atcacgattc tcaagatgtt
     1921 gctaattttt ttggaattca gcaatctttt gcatttgatg gtgttcgagg tgagaaagtg
     1981 agtgtatctc ttcttgtttc gaaagatggt ggcgatagca acagtggctt gaaagtcgct
     2041 ttgcactaca tcaaaaacaa aaacattatt gggcaagagt ggcaaaatat cccaagtcca
     2101 caaataacat cgaagtataa gcgtttcaca tttacgttta ctttatcaga cgatgtagag
     2161 aatctgaatt tgatgttgtt tggtgaaaaa gggaagacca tcaacctcta tgttacagat
     2221 gttcaactcg aaagaggttc tgtcgcgacg gattacaaag aagctcctga agacacggac
     2281 gaagcgattc gctctgttca aagccaacta actggctcat gggcagttca aaacatcaac
     2341 tcggctggag atatcatctc tggaatcaat cttggcgcca atggacataa ccgctttgtt
     2401 gggaaattga cccacatcac tggagagacc ctgattgaca gagcagtcat caagtctgcc
     2461 atggttgata agctcaaaac ggccaatttt gaagctggtt cggtcacgac tacgatatta
     2521 gacgctgaag cggtaactgc tgagaagttg aaagttgaca atgcgcttat tagaaaatta
     2581 actgcaaatg atgcttttat tgaccaactg atatctaaac gtatcttctc tactaaggtt
     2641 gagtccgtca tttctagctc aaccttccta gaagcctatc aaggccgaat cggtggattc
     2701 acacttggtc aatttgacca gggtggcggt cgctggattt caggtgtcaa tcagttctct
     2761 gttggtatgg ggaatggtgc cgggtatgga gtccggacag ccttctgggc gaactgggga
     2821 aataattgga actatgccgg acctaaagca tggaacgtca atactgatgg gaaaatgtac
     2881 tgtaggaatg aagtcggttt ttatgatcaa gtggattttt cgaattcatc gagagcaaac
     2941 ttctatggga atactacttt ttctcgttct cctgtgtttt caaatggtat cgaacttgga
     3001 agtaaagatg tgcttggtga tggttggaat cccaaaggcg gaaggaatgc ggttgtttgg
     3061 tggaatcagg tcggtagcgg tagcgtgaag tattggatgg aacaaaaatc agacagacgc
     3121 ttaaaagaga acatcacaga tacagctgtg aaagccttgg acaaaatcaa cagattaaga
     3181 atggttgcat ttgatttcat cgaaaataaa aaacatgagg agattggtct aatagctcaa
     3241 gaggctgaaa ccatcgttcc aaaaattgtc tcacgagatc ctgagaatcc agatggctat
     3301 ctgcatatcg actataccgc tttagttcct tacttaatca aggctattca agaattaaat
     3361 caaaaaatag aaaaaatgga gaaaacaata gcatgaataa caacatgttg accaatatcg
     3421 cacttaaagc aatttaggag cttgctcttg aaaatagaaa acgaacacac agattggaga
     3481 acttagaaaa tgaacacaga acagcttaac caagccttac aaatgacaat tagtgaaatg
     3541 tcaacaactt caacaaattc gatgattaca agtaatatct tgagtattca gttgaatgag
     3601 caaagggaag agaatcaaag acttcaagca cgagtggatg agctggaagc tctgcttgat
     3661 gaacaaacta aaccagcaga caaaggagaa tagacatggc agaaacaatt caaaacacag
     3721 ataacttact agaccttaca aaaatcacag aaccatttga tcttgcgagc gctttgcgct
     3781 acatgaaaga aaatggagag ttcattcgtt gcaagaatgt aagcgatgac ttctatatgt
     3841 atcgtgacgt tcaaaaacgt cctgtgatcg taaatggccg tcgccaattc aaggatgttg
     3901 aaaccgtttg ggcgttcaat cagtggggtg gtacaatcgc aacaatcaac gtagccgttc
     3961 tgttgaatca tgaattctat atcatgaaat ttgatgcaga gggcaatcct gactggacgg
     4021 ttccaacggt agaacctaaa gaataggagg ttgtatgcaa attgaatttt tcaattttct
     4081 aagaagtgtc gtacagactg aagatggttt ggtattgtac gctctagcac tgattgtctc
     4141 aatggaaatc attgattttg tgacagggac gattgcggca attatcaatc ctgacatcga
     4201 gtacaagagc aaaatcggca tcaatgggct atttcgtaag atttcagggg ttctcttact
     4261 gatgatcctc attccggcgt ccgttttgtt gcctgaaaag acaggttttg cattcttgta
     4321 ctcaatctat ctcgggtaca tcgcatttac ttttcaatct ctcattgaaa attaccgcaa
     4381 attaaaagga aatgttactc tttttcagcc gattgtaaaa gtatttcagc gattacttga
     4441 aaaagatgat gatacgaaaa aaggagaata acaaatgcaa caaattactg aaatcattac
     4501 taatggagca atcagcatcc ttgttatttt ggctggtatc gcagtcaagg cagtcaagga
     4561 ctacctggtt caaaaaggtg gagaaaagac catcaaaatc gttgaaatct tggccaaaaa
     4621 tgcagtaaat gccgtggagc aggtagctgc tgaaactggc tacaagggag atgaaaaact
     4681 ggcacaggct cgtgctaaag tccgtgctga gcttacaaaa tacaatatta gcatgactga
     4741 caaaaactta gacaccttcg tagagtcagc agtgaagcag atgaatgacg tatggaaagg
     4801 acgataggga atggatatcg atagaaacag actacgtaca ggcttgccac aggttggggt
     4861 gcagccttat cgacaagtac atgctcactc aacaggtaac cgcaactcaa ccgtacagaa
     4921 tgaagcggat tatcactggc ggaaagaccc agaattaggt tttttctcgc acgttgttgg
     4981 gaacggccgt gtcatgcaag taggtcctgt gaacaacgga agttgggatg ttgggggcgg
     5041 ttggaatgct gagagttacg cagcggttga actgattgaa agccattcaa ctaaggaaga
     5101 gtttatggct gactaccgcc tctatatcga attgctacgc aatctagcgg acgaagcagg
     5161 cttgccgaag actcttgata cagacgactt ggcaggtatc aagacgcatg aatactgtac
     5221 caataaccaa ccaaacaacc actcagatca tgtggatcca tacccttact tgacaaaatg
     5281 gggcattagc cgtgaacagt ttaagcaaga catcgaaaac ggcttgagcg ctgcaacagg
     5341 ctggcagaaa aatggcactg gctactggta cgtacactca gacggctctt atccaaaaga
     5401 taagtttgag aaaatcaacg gtacctggta ttatttcgat ggctcaggct atatgctttc
     5461 agaccgctgg aagaagcaca cagacggtaa ttggtactac tttgaccaat caggcgaaat
     5521 ggctacaggc tggaagaaaa tcgctgagaa gtggtactat tttgatgtag aaggtgccat
     5581 gaagacaggc tgggtcaagt acaaggacac ttggtactac ttagacgcta aagaaggcgc
     5641 catggtatca aatgccttta tccagtcagc ggacggaaca ggctggtact acctcaaacc
     5701 agacggaaca ctggcagaca agccagagtt cacagtagag ccagatggct tgattacagt
     5761 taaataaaat agaaaggaaa ctttctaaat tgttctttca ccgcaggctc aggcttgcgg
     5821 tttttttgtt tgtctgaatc aagaaaacgt ctaaccaacc gacaccaatg tcggtagcaa
     5881 aataaatggt ttgcctaaaa aattagagtt aagttctatt tttcaaaaac acgcattttg
     5941 aacgattaga aacagaaatc taaatcctat tgttcaaaaa aacgtttaca tgaaaaatag
     6001 ggaggtgttt tgttaaaaat aaaaacagtg atcgaaatca ctgcttatca gttgtagcaa
     6061 attcataaag tttttctgct gtgagaagtg ccattttgtc catgcttgtt tttccttttc
     6121 tgaggtcaga aacagtagtc catggaactc cagcaccttg ggaaatagca gatgtagaca
     6181 tcgaactgtc taacaattct tgaataactt ctctcatctt atttgtcctt tttatttttt
     6241 agataaatat atacattgat tgcaattata aaaatagcta ttgcactaac cattgcattt
     6301 cctctttcca tttgataaaa tagaggtgta aggggctttc gccccaacct cttagcgttt
     6361 acctttttct ttgacgggat ttcggtttac gctttttgtt ttgccttgcg accgttattg
     6421 cggtcactag acttgcgata gcagttaccg tttcaggaat attgtctatt gccttttcaa
     6481 gtaacctgag ccaatcttct ttgttcaact tcatcacccc ctttccttat cttgattata
     6541 ttatatcacg gtacaccgag aaagtcaagc gttttgatga agttttttta attttttcaa
     6601 aaaaaaaata gaccttgtcc agaggtcggg gagttggagg ggacaccctc caagagtgtt
     6661 gatttaataa gattttattt ta
//
LOCUS       DAVHEX010000084         6592 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVHEX010000084 DAVHEX010000000
VERSION     DAVHEX010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6592
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.085"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..159
                     /locus_tag="VVL45_001982"
     CDS             <1..159
                     /locus_tag="VVL45_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU4188108.1"
                     /translation="EEFGHKLLPLPPYSPEYNPIEKTWAHIKKHLKKVLPSCNTFYEA
                     FLSCSCFN"
     gene            220..1182
                     /locus_tag="VVL45_001983"
     CDS             220..1182
                     /locus_tag="VVL45_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU4188109.1"
                     /translation="MNMSNITIDGQEMDLSQTMPSYAQVVSLYDDTSISVSNNETDKV
                     LAGSLYTDTNEQGLTIPSSLLKNWNEQTGKNLTANDLIGKSVSASIVESAAETSKIAQ
                     FQTKIVRVINDEDDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPSQTKVVTE
                     ELQKNKKYTVLSQQRVLDIVITFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRS
                     KEIGVMKAVGYQNRDVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSIT
                     KVFELNLLSVLGTLVFALLLGYVSAYFPARKISKMDPIESLCYE"
     gene            1359..2366
                     /gene="gap"
                     /locus_tag="VVL45_001984"
     CDS             1359..2366
                     /gene="gap"
                     /locus_tag="VVL45_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEU4188110.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFIKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2434..3270)
                     /locus_tag="VVL45_001985"
     CDS             complement(2434..3270)
                     /locus_tag="VVL45_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU4188111.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWKEIVNPGD
                     VCQLTFDEEDYSQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGQYAETHVSRLKQFSNKTSLAHCKLKTGRTHQIR
                     VHLSHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLEKLTFTTLSNTFEKELKK
                     NG"
     gene            3396..5591
                     /locus_tag="VVL45_001986"
     CDS             3396..5591
                     /locus_tag="VVL45_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEU4188112.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETKAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEASWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYVMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            5644..5796
                     /gene="rpmG"
                     /locus_tag="VVL45_001987"
     CDS             5644..5796
                     /gene="rpmG"
                     /locus_tag="VVL45_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU4188113.1"
                     /translation="MALKKASLACAVCGSRNYSIKISENLKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            5806..5982
                     /gene="secE"
                     /locus_tag="VVL45_001988"
     CDS             5806..5982
                     /gene="secE"
                     /locus_tag="VVL45_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEU4188114.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            6037..6573
                     /gene="nusG"
                     /locus_tag="VVL45_001989"
     CDS             6037..6573
                     /gene="nusG"
                     /locus_tag="VVL45_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEU4188115.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAEL"
BASE COUNT         2049 a         1161 c         1424 g         1958 t
ORIGIN      
        1 gaagagtttg ggcataaact tttacctctt cctccctact cgcctgagta caatcctatt
       61 gagaaaacat gggctcatat caaaaagcac ctcaaaaagg tattaccaag ttgcaatacc
      121 ttttacgagg cttttttatc ctgctcttgt ttcaattgac tatatatcac ggatacagtt
      181 gggaaagaag ctattaaata tttggagagt ccttttggga tgaacatgtc aaatattact
      241 atagatgggc aagaaatgga tctgagtcaa acgatgcctt cttatgctca agtagtgagt
      301 ctttatgatg atacaagtat ctctgttagt aataatgaga cggacaaagt gttggctgga
      361 tccctctata ctgatacaaa tgaacaggga ttaacgattc caagcagttt actaaaaaat
      421 tggaatgaac agacaggaaa aaatttgaca gctaatgatc ttattggcaa atcagtctca
      481 gccagcattg tagaaagtgc tgccgaaact agtaagattg ctcaatttca aacgaagatt
      541 gtacgtgtaa tcaatgatga agatgacatg gaggacagca acagtttcat gctgtctcat
      601 caaatggaaa cgattttgaa agaggctgga tttacgaaag ctgtatctta ttttatcttg
      661 gaactcaaag atccatcaca gacaaaagta gtaacagaag aattacagaa aaataagaag
      721 tatactgtgc tttctcaaca gagagttctt gatattgtga ttacctttat tcgtgttatt
      781 cagggattat tgattgtgct ttcatcacaa gctattgtgg tagcagcggt tatgattggt
      841 atcattattt acatcaatat catgcaacgt tccaaggaaa taggtgtcat gaaagcagtt
      901 ggttatcaga atcgtgatgt caaaggaatt tttatttacg aggctatctg gattgtaggc
      961 atcgccttgc tgctggcatt tttggttgca caaggggtgg gaagtttggc gaatgcgatt
     1021 gtaagtcact tttacccatc catcactaag gtttttgaat taaatctttt atctgtttta
     1081 ggaactctag ttttcgctct attacttggt tatgtctcag cctacttccc ggcgcgtaag
     1141 attagtaaaa tggatcctat agaatcgtta tgctatgaat agtaagggat tccttggttt
     1201 acagtcttaa aaagaaacta acatagaaaa acaatggcca tacttgcaat tcaactcaaa
     1261 tagttgtata ataggtttgt tgaaaggtga tttgtagtga ttcaagttac tcttttcaca
     1321 ataaaaattt catgattttc ataaggagga aatcactaat ggtagttaaa gttggtatta
     1381 acggtttcgg acgtatcggt cgtcttgctt tccgtcgtat ccaaaacgta gaaggtgttg
     1441 aagttacacg catcaacgac cttacagatc cagttatgct tgcacacttg ttgaaatacg
     1501 acacaactca aggtcgtttc gacggtactg ttgaagttaa agaaggtgga tttgaagtta
     1561 acggtaaatt catcaaagtt tctgctgaac gtgatccaga acaaatcgac tgggctactg
     1621 acggtgtaga aatcgttctt gaagctactg gtttctttgc taagaaagaa gcagctgaaa
     1681 aacaccttaa aggtggagct aaaaaagttg ttatcactgc tcctggtgga aacgacgtta
     1741 aaacagttgt attcaacact aaccacgacg ttcttgacgg tactgaaaca gttatctcag
     1801 gtgcttcatg tactacaaac tgcttggctc caatggctaa agctcttcaa gacaactttg
     1861 gtgttgttga aggattgatg actactatcc acgcttacac tggtgaccaa atgatccttg
     1921 acggaccaca ccgtggtggt gaccttcgcc gtgctcgcgc tggtgctgca aacatcgttc
     1981 ctaactcaac tggtgctgca aaagctatcg gtcttgtaat cccagaattg aatggtaaac
     2041 ttgacggatc tgcacaacgc gttccaactc caactggatc agttactgaa ttggtagcag
     2101 ttcttgaaaa gaacgttact gttgatgaag tgaacgcagc tatgaaagca gcttcaaacg
     2161 aatcatacgg ttacacagaa gatccaatcg tatcttcaga tatcgtaggt atgtcttacg
     2221 gttcattgtt tgacgcaact caaactaaag ttcttgacgt tgacggtaaa caattggtta
     2281 aagttgtatc atggtacgac aacgaaatgt catacactgc acaacttgtt cgtactcttg
     2341 aatacttcgc aaaaattgct aaataattct tgagttgata gaaagcaagg ctttgtggtc
     2401 ttgctttttt atatggaaaa atggatgaca cgatcatcca ttctttttta attctttttc
     2461 aaatgtattt gaaagggtag tgaaagttag cttctctaaa gtaagtgggt gggtaaagga
     2521 aagtcggaag gcatgaagca taagccggct tgtctttgat ttactattat agagagggtc
     2581 tcccaggata ggaagattat gatgcgaaag gtgcacacga atctgatggg ttcgccctgt
     2641 ctttagcttg caatgagcca aggaagtctt gtttgagaat tgctttaatc tgcttacatg
     2701 cgtttcagca tattgcccat tttttgcatc aactattctt tttctacgat catggcgatc
     2761 acgtccaatt ttgtctctga aaacaagttc ttttctgttg atatttccat caactagagc
     2821 ccaatattct ctagaaatct cttttttctc caataagcga ttgagaatgg gcaggataaa
     2881 aggatttttg gcaaagagaa ctaagccact ggtttccatg tccagacgat gaacgacata
     2941 gcaggtttgg ccaacatagg tactgacatg gttaagaagg gcaatttcgt ttggttgatt
     3001 accatgcgtt ttcatcccct ctggtttgtt tacaataatc aagtgttgat cttgataaac
     3061 ttcctgcact aagtctgggt tgccccaagg gatcgtcttt tgggaataat cttcctcgtc
     3121 aaaagtcaac tggcaaacat ctccaggatt tacgatttcc ttccagtgga cttcttcttg
     3181 atttatcaaa atatgtttct tgattctcaa aaaatgacgg atttttctag ggatgaggag
     3241 ttgttcctca agtaattgct ttactgtcat ttgaggtaga gaggcgggta atgtaaatgt
     3301 gaattgcata cagatattgt aacaaaaaaa gccctatttg gataggaaat agctaaattc
     3361 ttgtcttcct atgatgaaga tgataaaata aacgcatgaa attagataaa ttatttgaga
     3421 aatttctttc tctttttaaa aaagaaacaa gtgaactaga ggactctgat tctactatct
     3481 tacgtcgctc tcgtagtgat cgaaaaaaat tagcccaagt aggtccgatt cgaaaattct
     3541 ggcgtcgtta tcatctaaca aagattatcc ttatactagg tttgagtgca ggcttgctag
     3601 ttggaatcta tttgtttgct gtagccaagt cgaccaatgt caatgatttg caaaatgcct
     3661 tgaaaactcg gactcttatt tttgaccgtg aagaaaaaga ggctggtgcc ttgtctggtc
     3721 aaaagggaac ctatgttgag ctgactgaca tcagtaaaaa cttgcagaat gctgttattg
     3781 cgacagaaga ccgttctttc tataaaaatg acgggattaa ctatggccgt tttttcttgg
     3841 ctattgtcac tgctggacgt tcaggtggtg gctctaccat tacccaacag ctggctaaaa
     3901 acgcctattt atcgcaggat caaactgttg agagaaaagc gaaagaattt ttccttgcct
     3961 tagaattaag caaaaaatat agtaaggagc aaattctaac catgtacctt aacaacgctt
     4021 attttggaaa tggtgtgtgg ggtgtagaag atgcgagtaa gaaatacttt ggagtttctg
     4081 catcagaagt gagtctggat caagctgcga ctctggcagg gatgctcaag gggccggaac
     4141 tgtataatcc cttgaattcc gtagaagatt ctactaatcg gcgcgatact gtcttgcaga
     4201 atatggttgc agcaggatat attgataaaa accaagaaac caaagctgct gaagttgata
     4261 tgacttcgca attgcacgat aagtatgaag gaaaaatctc agattaccgt tacccctctt
     4321 attttgatgc ggtggttaat gaagctgttt ccaagtataa tctaacagag gaagagattg
     4381 tcaataatgg ctaccgcatt tacacagagc tggaccaaaa ctaccaagca aatatgcaga
     4441 ttgtttatga aaacacatcg ctatttccga gggcagagga tggaacgttt gctcaatcag
     4501 gaagtgtagc cctcgaaccg aaaacagggg gagttcgtgg agttgtcggt caagttgctg
     4561 acaatgataa aactggattc cggaatttca actatgcaac ccaatcaaag cgtagtcctg
     4621 gttctacaat taagccttta gttgtttata cgccagcagt tgaagcaagc tgggctttga
     4681 ataagcagtt ggataaccat accatgcagt atgacagcta taaggttgat aactatgcag
     4741 ggatcaaaac gagtcgagaa gttcctatgt atcaagcctt ggcagaatcg cttaatctac
     4801 ctgctgttgc cactgttaat gatttgggtg tcgacaaggc ttttgaggca ggcgaaaaat
     4861 tcggactcaa catggaaaag gtcgaccgtg ttcttggtgt cgccttggga agcggtgttg
     4921 aaaccaaccc tcttcaaatg gctcaagcat acgccgcctt tgcaaatgaa ggtttaatgc
     4981 ctgaagctca ttttattagt agaattgaaa atgctagtgg acaagttatt gcgagtcata
     5041 aaaattcaca aaaacgggtg attgataagt ctgtagctga caagatgacc agtatgatgt
     5101 tggggacttt caccaacggt accggtatta gttcatcgcc tgcagactat gtcatggcag
     5161 ggaaaactgg aacaactgaa gcagttttca atccggagta cacaagtgac cagtgggtaa
     5221 ttggttatac tccggatgta gtgattagcc actggcttgg ctttccgacc actgatgaaa
     5281 accactatct agctggctct acttcaaacg gtgcagctca tgtctttaga aacattgcca
     5341 ataccatttt accttatacg ccaggaagta cctttacggt tgaaaatgct tataagcaaa
     5401 atggaattgc accagccaat acaaaaagac aagtacaaac caatgataat agccagacag
     5461 atgataattt gtctgatatt cgagggcgtg cgcaaagtct agtagatgag gctagccggg
     5521 ctatctcaga tgcgaagatt aaggaaaagg ctcaaacaat atgggattcg atagtcaatc
     5581 tatttcgcta agatgcttgt caaagcctag ctttcttgtt ataatggata agatggaggc
     5641 gttatggcac taaaaaaagc aagcctagct tgtgcggttt gtggttcgag aaactattca
     5701 atcaagatca gcgaaaacct caagcctaca cgactagaag taaataaatt ttgtaagcat
     5761 tgtggcaagt acactacaca cagagaaacg agataggaga gagcgatgcg ttttattgga
     5821 gatattttta gacttcttaa agacacaaca tggccaactc gcaaggaaag ctggagagat
     5881 tttcgttcta tcatggaata cacagctttc tttgtagtaa ttatttacat ttttgaccag
     5941 ttgattgttt caggtttgat tcgatttatt aacatttttt agaagattag tggagttaat
     6001 tacactagaa atcttctatt tatgaaagga aatatcatgg atagttttga taaagggtgg
     6061 tttgttttac aaacttattc tggttatgaa aataaggtga aagaaaatct attacaacgt
     6121 gcacaaacct acaatatgtt ggataatatt ctacgcgttg aaattccaac acaaacagtg
     6181 caagttgaaa aaaatggaaa gagaaaagaa gtagaagaaa atcgctttcc aggttatgtt
     6241 cttgtagaaa tggtcatgac agatgaagct tggtttgttg ttcgaaacac accaaatgtt
     6301 acaggatttg tcggatcaca cgggaacaga tcaaaaccaa ctccattatt ggaacaagaa
     6361 attcgcgaca ttttggtatc tatgggacaa actgttcaag aatttgattt cgatgttgag
     6421 attggtcaaa ctgtacgtat tattgatggt gcttttgcag actacactgg taagattaca
     6481 gaaattgata ataacaaagt gaaaatgatt atctctatgt ttggtaatga cacagttgca
     6541 gaagtaaatc taaaccaaat cgcagaatta taaccctgag agaggctgga gc
//
LOCUS       DAVHEX010000085         6412 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVHEX010000085 DAVHEX010000000
VERSION     DAVHEX010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6412
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.086"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(26..1018)
                     /locus_tag="VVL45_001990"
     CDS             complement(26..1018)
                     /locus_tag="VVL45_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU4188116.1"
                     /translation="MSYYDFVKDYMKVDECKNNEKYSSAGHTFCWKKENSTYAEGLYW
                     FYEGGSFIIDIHDFYIKEEVVQNSTYSMSDYVSIYSSYIVSANGEKFNPYQTITANSL
                     CTFDFDNIKDNFVFLLHENCCYLAVSIGFKKELIEKHLASININPESFYSTLLQPNQI
                     ILTKALERVAMEILNCKMDAPAADFFFKAKANEWISIVIDTYLNRKKYKIEIDDDKAL
                     EDVARFLDDHFATNVNQGTLEKISKMSGTKLKNLFKEKYCQSITEYTQRKRMNVAETL
                     LLNTELPIKEIAESVGYSSASKFSIYYKRYKGKLPSEVRNLACKHHNFKCDYCD"
     gene            1178..2932
                     /locus_tag="VVL45_001991"
     CDS             1178..2932
                     /locus_tag="VVL45_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4188117.1"
                     /translation="MKIYKKLFAYVQDKKYLGVLAIIFSAISAALTVYGYYLIYKFLD
                     KLIINSNLSGAESIALKSVITLTSGAIFYFVSGMFSHILGFRLETNLRKRGIDGLEKA
                     SFRFFDLNPSGQIRKIIDDNAAQTHQVVAHMIPDSSQAIITPVLVLALGFIVSIRVGI
                     ILLALTIIGGLILGAMMGEQEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFK
                     SFYKAIKDYSKYAYDYSLSCKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAKVILLE
                     LIMILFLSGVLFVSFMRMMWYSMYISQGNYAVDTLEALYEDMQKDKLVHGNVNNLKNY
                     NIEFENVSFAYNDKAVIENLSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNKGSI
                     KIGGIAISEYSDEALIKAISFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGC
                     DLILDKFPERENTIIGSKGVYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEF
                     ELQKAFKNLMKDKTVIMIAHRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSL
                     QEMFNSANEWRVSNERVL"
     gene            2916..4636
                     /locus_tag="VVL45_001992"
                     /pseudo
     CDS             2916..4636
                     /locus_tag="VVL45_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214631.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            4647..5276
                     /locus_tag="VVL45_001993"
     CDS             4647..5276
                     /locus_tag="VVL45_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEU4188118.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYP
                     HMALVALGAFLGGILGAYIGKALLKKHFSNGLYCVGYFTPCLILWCYLN"
     gene            5177..5734
                     /locus_tag="VVL45_001994"
     CDS             5177..5734
                     /locus_tag="VVL45_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU4188119.1"
                     /translation="MYRQGSIEKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIP
                     IVIRMFILPFMAASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKN
                     IKMAMRVRGINFKNPVKYLEYVSVPLLIISSNISDDIAKAAETKAIENPIAKTRYIRV
                     KIQLIDFVYVLAVAGLIVGGLIWLK"
     gene            5722..>6412
                     /locus_tag="VVL45_001995"
     CDS             5722..>6412
                     /locus_tag="VVL45_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4188120.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGNGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFYLENIGLAREEMDRRLKDILEIFPIKNLLNRNIFNLSGGEKQILCIAASYIAGTKI
                     IVMDEPSSNLDIKSISVLAKMLKILKEKGISIIVAEHRIYYLMDIVDRVFLIDKGKLK
                     KTYTRSEFLKLD"
BASE COUNT         2283 a          863 c         1140 g         2126 t
ORIGIN      
        1 aaaacatggc ttgtaaaaaa ttactttaat cacaataatc gcatttaaaa ttgtgatgtt
       61 tgcaagctaa attacggact tcacttggaa gttttccctt gtatctttta taatagatag
      121 aaaatttgct ggcagatgaa tatccaacag attctgctat ctcttttata ggtagttcag
      181 tgtttaaaag aagagtttca gctacattca ttctttttct ttgagtgtac tctgtaatgc
      241 tttgacaata tttttcctta aataaatttt ttaatttagt accactcatt ttagatattt
      301 tttcaagcgt gccttgattt acattcgttg caaaatgatc atctaagaat cttgctacat
      361 cttcaagtgc tttatcatca tcaatttcaa ttttatattt ttttctattt aagtatgtgt
      421 caattactat acttatccat tcatttgcct ttgctttaaa gaaaaaatca gcggcaggag
      481 cgtccatctt acaatttaat atttccattg ccactctttc taaggccttt gtaagtatta
      541 tttgattcgg ttgaagcaag gttgaataaa aagattctgg attaatgtta atagatgcta
      601 aatgtttttc tattagctct tttttaaaac caatggaaac agcaagataa caacaattct
      661 cgtgtaataa aaaaacaaaa ttatctttta tattatcaaa atcaaaagta catagagagt
      721 ttgcggtaat agtttgatac ggattaaact tttctccgtt tgcactgaca atgtaacttg
      781 aataaattga aacatagtct gacatactat aagtgctatt ttgaactact tcctctttga
      841 tataaaaatc atgtatatcg ataatgaagc tgcctccttc ataaaaccaa tatagtcctt
      901 ctgcataagt tgaattctct tttttccaac aaaatgtatg acctgcactt gaatacttct
      961 cattgttttt acattcatct actttcatat aatcttttac aaaatcataa tatgacataa
     1021 cacactatcc cttttagaca atattccaat tagccttatt aattcaaaac tattgtatta
     1081 gtaattataa cagatgtata atagaaaagc aatgatagat attatcaatt aagcgaattt
     1141 atatctaaaa gggatattaa agaaaggaga tatgcttatg aagatttaca aaaaactatt
     1201 tgcttatgtc caagataaga aatatcttgg ggttttggcc ataatttttt ctgctatatc
     1261 tgctgcactt acagtatatg gatattattt aatctacaaa tttctagata agttaataat
     1321 taattcaaac ttatccggtg cagagagtat agcattaaaa tctgttatta cactaacaag
     1381 tggagcgata ttttattttg tctcaggaat gttttcacat atcttgggat tcaggcttga
     1441 aacaaattta agaaaaaggg gaatcgatgg tctggaaaaa gcaagtttta ggttctttga
     1501 cttaaatcca tctggtcaaa taagaaagat tatagatgac aatgctgcac aaactcatca
     1561 ggtggtagca cacatgattc ccgatagttc tcaggcaata atcacacccg tacttgtact
     1621 tgcacttggc tttatagtaa gtataagagt tggcataatt ttgcttgctc ttactataat
     1681 tggtggctta attttagggg caatgatggg cgagcaagaa tttatgaaga tataccaaga
     1741 atccctatct aaactaagtg ctgaaactgt tgagtacgtg agaggaatgc aagttgtaaa
     1801 aatatttaaa gcaaatgtag agtcttttaa aagcttttat aaggcgataa aagattactc
     1861 aaagtatgct tatgattatt ccctatcttg taaaaggcct tatgttttgt atcaatggtt
     1921 attttttgga ctgattgcaa ttttaattat tcctatagtt tattttatga ctagcttagc
     1981 tagcgcaaag gtgattttac ttgagcttat catgatttta tttttatcag gagttctctt
     2041 tgtttcattc atgagaatga tgtggtactc catgtatatt tctcaaggaa attatgcagt
     2101 agatacttta gaggcgcttt acgaagatat gcaaaaagac aaattagtgc atggtaatgt
     2161 caataattta aaaaactata atatagaatt tgagaatgtt agctttgctt ataatgataa
     2221 agctgtcatt gaaaatttat cctttaattt agaagaagga aagtcctacg cacttgtcgg
     2281 ttcatctgga tcaggcaaat caacagtagc aaaacttata tcaggttttt acaatgttaa
     2341 taaaggaagc ataaagatag gcgggatagc aataagtgaa tattctgacg aagccttaat
     2401 taaagccatt tcctttgttt ttcaagattc aaaattattc aagaagagca tttatgataa
     2461 tgtagcgtta gctaataaag atgcgacgaa agatgacgtt atgagagcct taaaattagc
     2521 aggatgcgat ttaatattag acaaattccc agaaagagaa aatacaatca taggctcaaa
     2581 aggtgtttat ttatccggtg gagaaaaaca aagaattgca attgctagag caattttaaa
     2641 ggattccaaa attattatta tggatgaagc atcagcatct attgacccag ataacgagtt
     2701 tgaattgcaa aaagctttta aaaatcttat gaaggataaa acagttatca tgattgcaca
     2761 caggctatct acaattaaag accttgatga aattattgtc atggatagtg gaaaaattat
     2821 agaaagaggg tctgacaaag aattaatgtc aaaagataca aggtataaga gcctgcaaga
     2881 gatgtttaac agtgcgaatg aatggagggt ttcaaatgaa agagttttat aaaaaaagat
     2941 ttgctcttac agatggagga gcaagaaatt taagtaaagc aacactggct tcatttttcg
     3001 tttattgtat aaacatgctt cctgccatat tacttatgat ttttgctcag gaagttttgg
     3061 aaaatatggg caaaagcaat ggcttttata tagtattctc agttttgatt ttgatagcaa
     3121 tgtatatttt gctttctatc gaatacgata aattatataa cacaacctat caagaaagtg
     3181 cagatttaag aataaggaca gcggagaatt tatcaaaatt acctctatct tacttttcta
     3241 aacatgacat ttccgacatt tcacaaacaa tcatggctga tattgaaggc atagagcatg
     3301 caatgagcca ctcaatacca aaggtgggcg gcatggtact gtttttccca ttaatatctg
     3361 taatgatgct agcgggcaat gtcaagatgg gtttagctgt aattattcca tctattttaa
     3421 gctttatatt tataccttta tctaaaaaat atcaggttaa tggacagaat agatattatg
     3481 atgtcttaag aaaaaactca gaaagctttc aagaaaatat cgaaatgcaa atggagatta
     3541 aagcatataa tttatcgaag gatattaaag atgacttata taaaaaatgg aagatagtga
     3601 gaaagtacac ttaaaggcgg aagtaactac aattttaact ttgtctatat cttcaatatt
     3661 tagctttata tctcttgctg ttgtgatatt tgtcggcgta aatctaatta ttaataaaga
     3721 gataaattct ctctacctta taggatattt actagctgct atgaagataa aagactcttt
     3781 agatgcatct aaagagggct tgatggaaat attttattta tcgcccaaaa tagaaagatt
     3841 aaaagaaatt caaaatcaag atttacaaga aggcgatgac tatagcttaa aaaaatttga
     3901 tattgatcta aaagatgttg agtttgccta caataaagac gcaaaagttt taaatggtgt
     3961 aagttttaaa gctaagcagg gagaggtcac tgctttggta ggtgcaagtg gctgcggtaa
     4021 aacaactatc ttgaaactta tatcaagact ttatgattat gacaagggac aaatcttaat
     4081 cgatggcaaa gatataaagg aaatatcaac agaatccctt tttgataagg tgtctattgt
     4141 tttccaagat gtggttctct ttaatcaaag cgttatggaa aatattagaa tcggtaagca
     4201 agatgcaagt gacgaagagg ttaaaagagc agcaaaactt gcaaattgca cagattttat
     4261 agaaaaaatg gataaaggtt tcgatacagt tattggtgaa aacggagctg agctatcagg
     4321 aggagaaaga caaagattat caatagccag agccttctta aaagatgcgc cgatattgat
     4381 cttagatgag ataacagcaa gccttgatgt taacaacgag aaaaagattc aagagtcttt
     4441 aaataattta gttaaagata aaactgttgt aatcatttca catagaatga aatccataga
     4501 aaatgcagac aagatagtag ttcttcaaaa cggaagagta gaaagcgaag gtaagcatga
     4561 agagctttta caaaaatcaa aaatttacaa aaatttaata gaaaagacaa aaatggcaga
     4621 agaatttatt tattaggagg actacaatgg ataataaaaa attaaaagta aaagatttag
     4681 taagcatcgg tgtttttggc gtaatttatt ttgccttcat gtttggagtt ggtatgatgg
     4741 gcttgattcc aatattgttc ttaatatacc cgacagtatt agccatagtt gcaggaactg
     4801 ttgttatgtt atttatggct aaggttcaaa agccatgggc actatttata tttggtatga
     4861 tatcaccact tgtgatgttt gcagctggtc atacctacgt agttgtggtt ttatcactta
     4921 tagtaatgat aatagcagaa ttaattagaa agattggtaa ttataattca tttaaataca
     4981 atatgctttc ttatgcaatc ttcagcacat ggatatgtag ctctttaatg caaatgcttt
     5041 tagcaaaaga aaaatatatg gagtggtctt tgatgactat gggaaaagat tatgttgatg
     5101 tattagaaaa gttaataact tatcctcaca tggctttagt agccttaggt gctttcttag
     5161 gaggaattct tggagcatat ataggcaagg ctctattgaa aaaacacttt tcaaatggat
     5221 tatattgtgt gggatacttt actccttgcc taattttatg gtgctatctg aattaaaccc
     5281 tatagttaag atgtttttga gtatacctat tgttattaga atgtttattt taccatttat
     5341 ggcagcaagc tttatgataa agacctcgga tgtaggcgca ataatttcat cgatggataa
     5401 gcttaagatt tcaaagaatg tatccatacc tattgcggtt atgtttagat tcttcccatc
     5461 ttttaaggag gagaagaaaa acatcaaaat ggctatgaga gtaagaggga taaattttaa
     5521 aaacccagtc aaatatcttg aatatgtttc tgtgccacta ctcattatat catctaatat
     5581 atcagatgac attgcaaaag cggcagaaac aaaggcaata gaaaatccaa ttgccaagac
     5641 cagatacatt cgcgtaaaga tacagctaat tgattttgtt tatgttttag cggttgctgg
     5701 acttattgtg ggaggcttaa tatggttgaa ataaaaaatt taagtcttga ttatggtgaa
     5761 gagcatatat tagatgatat atcactatcc atagccgagg gagagtgcgt gctatttaca
     5821 ggaaaaagtg gaaatggtaa gtcatcttta ataaattcaa tcaatggact agctgtaagg
     5881 tatgataacg caaagacaaa gggcgaaata attattgatg gtaagaatat aaaaaatttg
     5941 gaactttatc aaatctcaat gcttgtttca actgtttttc aaaatcctaa gacatatttt
     6001 tttaatgtca atacgacatt agaattatta ttttatttgg aaaatatcgg tcttgcaaga
     6061 gaagagatgg acaggcgttt gaaggatata cttgagatat tcccgataaa aaatcttttg
     6121 aacagaaata tatttaatct atccggcggt gaaaaacaaa ttctttgcat tgcagcttct
     6181 tatatagcag gtacaaagat tatagttatg gatgagcctt catcgaattt agatattaaa
     6241 agcataagtg ttttggcaaa gatgctaaag atattaaaag agaaaggcat aagcataatt
     6301 gttgcagagc atagaattta ttatttgatg gacatagttg accgtgtatt tttaatagat
     6361 aaaggaaagc ttaaaaaaac ttatactaga agtgaatttt taaagctaga ta
//
LOCUS       DAVHEX010000086         6329 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVHEX010000086 DAVHEX010000000
VERSION     DAVHEX010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6329
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.087"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            244..510
                     /locus_tag="VVL45_001996"
     CDS             244..510
                     /locus_tag="VVL45_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188121.1"
                     /translation="MEILSKEIQLQGLQLLKQTLETLVELEKQRSSKLDLISRKELMD
                     LLGISATTLDNWEDLGLKRYQTPMDGAKKVFYRPSDVYLFLAIK"
     gene            521..673
                     /locus_tag="VVL45_001997"
     CDS             521..673
                     /locus_tag="VVL45_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188122.1"
                     /translation="MKIVTFKPTKQIDDGFYLPGIDILFVSDKADAKDKEDVILFLSR
                     NGLNKS"
     gene            878..1495
                     /locus_tag="VVL45_001998"
     CDS             878..1495
                     /locus_tag="VVL45_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HEU4188123.1"
                     /translation="MELVYMDGKKEPYTTSEIIAECAEVQHHTITRLIRENKADFEEL
                     GILGFKIHKLDTRGQPKKSYILNEQQATFLITYLKNTETVRQFKLNLVKAFFEMREEL
                     SEIRLQRALEKPKRKTLHDSIETWPNAPKHAHSTMNNLLLKAVTDMNAKQLREERGGY
                     NGIDSLTSEELEQYQAFEDMAIAMIELKMSYQEIKTMMFRSKKIS"
     gene            1530..1982
                     /locus_tag="VVL45_001999"
     CDS             1530..1982
                     /locus_tag="VVL45_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188124.1"
                     /translation="MTETIYENLTRRIDRISAEMREISEENGLRILSLLESQTKSIKD
                     ELSRLLWIELPELNESHKIEAVSKSTTGMFFAPGIFEMDAMRKAFFKRQAKHFFENEA
                     EQQAYIEHAEKEYLEATVTLKDILFNSKNGTQKANKSCLIEKFEEAMQ"
     gene            1979..2425
                     /locus_tag="VVL45_002000"
     CDS             1979..2425
                     /locus_tag="VVL45_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEU4188125.1"
                     /translation="MTLDLDNMTQAEFDEVMAEIQLQSPNIFQLISDFVNKKVTSVEI
                     DDLLNMKRAETGKALSPLQIQELQYMLEDFSPELIHEALKEAVSQGKANFAYIKVILN
                     RWKQDNLLTVELVRNSRAAREAKKQQTNQLEPTSYEDWIPTQEKPF"
     gene            2437..3321
                     /locus_tag="VVL45_002001"
     CDS             2437..3321
                     /locus_tag="VVL45_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU4188126.1"
                     /translation="MKSLQSSLGNIETFKLDELCPKHEIQLTQIKGERHVVVGWNENG
                     EAIKEVRCIPPYCEQCQEEQKKQDEEDAIADNLNAKLYMQTYNVLMSNKSYVADEFKT
                     KTFDDFNAVTSEEKRFLEFAKGQVQKYLDGMRGNTLITGGTGIGKTLLSVAIAKGINE
                     GYKTKGEPKSVLFVSLTEMIKQIKEGWNYGKGATLTEFEATELMKSVDYLIIDDLGAK
                     NAVIKPKSDWEQDLLFDVLNNRENTIFNTNLDSDELKIVYNERNYSRILKGLEGNTFK
                     AFGIKYKRYSLNQLKSNM"
     gene            3570..4220
                     /locus_tag="VVL45_002002"
     CDS             3570..4220
                     /locus_tag="VVL45_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188127.1"
                     /translation="MTENKDDKLLKMMEKGFVLYSKNGIIKYIEIPEHGSIKLKAQDG
                     QLVYKEVIIGKVYAEQELLKYKKEGENYSQNTAQLLYQQLEKEKNAELANHESKEEPI
                     TADDAAELSLLSSIKLTVAEMREYLEKYKNKPLALRKLEDIMDNDTTLAYIEIDMEQF
                     SQQQRLEKLVHFLDRKIDYFHGGLLINGDKIDLVQHEMIVEGNSEAMDAELQNYLA"
     gene            4267..4695
                     /locus_tag="VVL45_002003"
     CDS             4267..4695
                     /locus_tag="VVL45_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEU4188128.1"
                     /translation="MAGNENDNLTSKQIKFIDAMLTEPTIDKACQKAGVSRATGHKYL
                     KVAAVKKTLRIKQDEMMDKTTQMLYLASSNAVSVLNDIMMDSKVNPFIRTQAAKAILE
                     QSYKTHEIFGVVRQIEELRLEIEEVSKGNQRVTRTQGVIK"
     gene            4703..5014
                     /locus_tag="VVL45_002004"
     CDS             4703..5014
                     /locus_tag="VVL45_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188129.1"
                     /translation="MPEFIIVEGNNDLGEFFQIDGELFSDNELLENLKKWREWEVPVI
                     IDDWCNRILNEDETEILYFPTHEDKMNYIRVEKDLEPLYHTSNKIYATISKSEWLELL
                     N"
     gene            5032..5442
                     /locus_tag="VVL45_002005"
     CDS             5032..5442
                     /locus_tag="VVL45_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188130.1"
                     /translation="MTKKKIERISVIHREKILWLKWYFMRDKEQPKYSVLERKMFDAA
                     KNQDMLAYQKYATIKQITDIRVQTSEADILEAVKEVYVYNHMNVIGACQRILFISQSP
                     AYDKLNKWFNIYSDLYFSVVPLPKMGVYHEMVGI"
     gene            5639..6004
                     /locus_tag="VVL45_002006"
     CDS             5639..6004
                     /locus_tag="VVL45_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU4188131.1"
                     /translation="MHNIYFYKDKNGNEPVFDYMRELTSKKGKDSRIKLNKINDYIEL
                     LSQHGTRAGEPYIKHLDAEIWELRPLRDRILFVAWMDGSFVLLHHFMKRTQKTPKREI
                     EQAKRELADLKERGLDNEK"
     gene            5994..6287
                     /locus_tag="VVL45_002007"
     CDS             5994..6287
                     /locus_tag="VVL45_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU4188132.1"
                     /translation="MKNNAIGSNWKDVRAELFSKEEILESDMRVAIMSELIEARNEKG
                     ISQKKLEEMSGVSQPVIARMETGKTSPQLDTVLKVLASLGKTLAVVPLEHEQV"
BASE COUNT         2407 a          909 c         1348 g         1665 t
ORIGIN      
        1 aagtatgcaa aagtaaagag tgcaaaaaat ggaattaaag cgaaaataaa agccgtgtac
       61 aggcgaccaa accaacgtac acggctaagg aaaaataaca aaactcaagc aaaggcaagg
      121 cgcgtggttt tgttaggtat ttagcaaggg gacaaacccc tttgtaaata atctcctctt
      181 attttatcaa aattagagga aaatgacaac ttaatttata aaaaggaaaa atggaggata
      241 taaatggaaa ttctgtctaa agaaatacag ttacagggct tacaacttct taaacagact
      301 cttgaaactt tagttgagct agaaaaacaa cgatctagta agttagattt aatttctcgt
      361 aaagaattaa tggatctgct aggtataagt gctacaaccc ttgataactg ggaggatctt
      421 ggtcttaaac gatatcagac tccgatggat ggagctaaga aagtattcta tcgtccgtca
      481 gatgtgtatt tatttttagc aataaaatag gagttatgaa atgaaaattg ttactttcaa
      541 accaactaaa caaatagacg atgggtttta tctgccaggt attgacattc tatttgtctc
      601 agacaaagca gatgccaagg ataaagaaga tgtaatttta tttctttcaa gaaatgggtt
      661 aaacaagtct tgaatgatat tagaaaataa atctactatt ccacggagga tatcgataca
      721 gcgatacaag tcgttataaa ggcctctaac acgtttatag agacaagtgg taaataatat
      781 taaccactag ccaaagccgt tttagaggct aaaaaagagg ctagaggagg caattaaaaa
      841 gtaagctaac cagatttata caggaggtag aaccgtaatg gaactagttt acatggacgg
      901 taagaaagag ccgtacacca caagcgagat catcgctgaa tgtgctgaag tacaacacca
      961 cactatcacg cgcttaataa gagaaaataa ggctgacttt gaagagttgg gaatacttgg
     1021 atttaaaatc cataaattag atactagagg acaacctaaa aaatcctata ttctgaacga
     1081 gcaacaggct acttttctga tcacttattt aaagaatacc gaaacagtac ggcaattcaa
     1141 actaaaccta gtcaaagcat tctttgaaat gcgtgaggaa ctttctgaaa ttcgtctgca
     1201 gagggcgcta gaaaaaccga aaagaaaaac cttgcatgac agtatagaaa catggcctaa
     1261 tgcaccaaag cacgcgcata gcaccatgaa caacttgcta ctaaaggcag taacagacat
     1321 gaacgctaag cagttaagag aagagcgtgg gggctataac ggtatcgata gcttgactag
     1381 tgaggagttg gagcagtacc aggcatttga ggatatggca atagctatga ttgaattgaa
     1441 aatgagttat caggaaatta agacaatgat gtttagaagt aaaaaaataa gctaaaaaat
     1501 cacaccaaca aagaaacaga ggtaaaaaca tgacagaaac aatatatgag aatttaacta
     1561 gacgtataga cagaattagc gcagaaatgc gagaaattag cgaagaaaat ggcctaagaa
     1621 tcctttcttt attggaaagt cagacaaaat ccatcaaaga tgagttgtcc cgtttacttt
     1681 ggattgaact tcctgaattg aatgaaagcc ataaaatcga agcggtctct aaaagcacta
     1741 cgggaatgtt tttcgcccct ggtatttttg aaatggacgc tatgcgaaaa gcattcttta
     1801 aacgccaagc taagcacttt tttgagaacg aagcagagca acaggcgtat atagagcatg
     1861 ctgaaaagga gtatttagag gctactgtaa ccttaaaaga tattcttttt aactctaaaa
     1921 atggaactca gaaagcaaat aaaagttgtc ttatagagaa gtttgaggag gcaatgcaat
     1981 gacactagat ttagacaaca tgacacaagc agaatttgat gaggtaatgg ctgaaatcca
     2041 gctacaaagt ccaaacattt ttcaactgat ttctgatttt gtaaataaaa aagtaaccag
     2101 tgtggaaata gacgacttgt tgaatatgaa gcgagctgag acaggtaaag cattgtcacc
     2161 gttacagatt caagaattgc agtatatgct agaagatttt agcccagagc ttatccatga
     2221 ggcgttaaaa gaggctgtca gtcagggtaa agcaaacttt gcatacatca aggtaatcct
     2281 taaccgttgg aaacaggaca atttattgac ggtggaactt gttagaaata gcagggcagc
     2341 gcgtgaggct aaaaaacagc aaaccaatca actagagcct accagctatg aagattggat
     2401 accaacacaa gaaaaaccat tttaggaggt cagaaaatga aatcattaca atctagttta
     2461 ggaaatattg agacatttaa actagacgaa ttgtgtccta agcatgaaat acagctaaca
     2521 caaatcaaag gagaaagaca tgtagtagtg gggtggaatg agaacggaga ggcaataaaa
     2581 gaagttcgct gtatcccacc ctattgtgaa caatgccaag aagaacagaa aaagcaagat
     2641 gaagaagatg cgattgctga taatttgaat gctaaactat atatgcaaac ttataatgtg
     2701 ttgatgagca ataagagcta tgtagcagat gagtttaaaa ctaaaacttt tgatgatttt
     2761 aatgctgtaa catcagaaga aaagcgattt ttagaatttg caaaaggtca ggtacaaaaa
     2821 tatcttgatg gcatgagggg aaatacgctt atcacaggag gtacagggat tggaaaaaca
     2881 cttttatcag ttgctattgc caaaggtata aatgaggggt ataagacaaa aggagaacct
     2941 aagagtgtgc tttttgtaag cctaaccgaa atgatcaaac aaattaaaga ggggtggaat
     3001 tatggtaaag gtgcaacact tacagagttt gaagctactg aactcatgaa gtctgttgat
     3061 tacttaatta ttgatgattt aggagcaaaa aatgctgtta ttaagcctaa gagtgactgg
     3121 gagcaggatt tactctttga cgtgctaaac aatcgtgaaa ataccatttt taataccaac
     3181 ctagatagtg atgagcttaa gatagtttac aatgagcgga attatagccg tattctaaaa
     3241 ggacttgagg gtaacacttt taaggcgttt ggcatcaaat ataagcggta ctcactcaat
     3301 cagttaaaga gtaatatgtg acaatgaatt aacttacaaa cctaaacaaa aatagacact
     3361 tttcatgggg tgagaaatcg ccctttgaga agtaatagcc atgctttcca cgaccatata
     3421 aaccaagtgt ggcaaactaa ccaatttgaa aagtcagcca taaggtacac aaaaagggta
     3481 gtattttagc atacgaaccc taaaaaccta gtgaaataaa tagactaggg gtattcagat
     3541 tataataaaa tgaaataaag gagaaaagca tgactgaaaa caaggatgat aaattattaa
     3601 aaatgatgga gaaaggcttt gttttatact caaaaaatgg tataattaag tatattgaaa
     3661 ttccagagca tgggagtatt aagctaaaag ctcaagatgg gcaattagtt tataaggaag
     3721 tgattatcgg gaaagtgtat gcagagcaag aacttttaaa atataaaaaa gagggcgaaa
     3781 actattccca aaatactgct cagctattgt atcagcaact agaaaaagag aaaaatgctg
     3841 aactagcaaa ccatgaatca aaagaagagc ctattacagc agatgatgca gctgaactaa
     3901 gtttattatc tagtatcaaa ttaacagtgg cagaaatgag agaataccta gaaaaatata
     3961 aaaataaacc tctagctctt agaaaattag aagatattat ggataatgat actacccttg
     4021 cttatatcga aattgacatg gagcaattca gccaacagca acgccttgaa aagctagtac
     4081 atttccttga tagaaaaatt gattatttcc acggtggttt acttattaac ggagataaga
     4141 ttgatttagt acagcatgaa atgattgttg agggcaattc agaggcaatg gatgcagaat
     4201 tgcagaatta tctagcatag ggaaataaag gggcaaccct ttattttgat aatgaggagg
     4261 taatagatgg caggaaatga aaatgataac cttacgtcca agcaaattaa attcatagat
     4321 gccatgctta ccgagccaac gatagacaaa gcatgtcaaa aagcaggggt atcacgcgca
     4381 acaggtcata agtatctaaa agttgcagcg gttaaaaaga cattgaggat aaagcaagat
     4441 gagatgatgg ataaaacaac tcaaatgctt tatttagcat catctaacgc tgtttctgta
     4501 ctcaatgata ttatgatgga cagtaaggtc aacccgttta taagaactca agcagcaaag
     4561 gctatacttg aacaatctta taagacccat gaaatttttg gagtagtaag gcagattgaa
     4621 gaattgaggt tggaaattga ggaagtatct aaaggaaatc aaagagttac aagaactcaa
     4681 ggagttatta agtaatagaa atatgccaga gtttattatt gttgaaggaa acaatgactt
     4741 aggggaattt ttccaaatag atggcgagct atttagtgat aatgaacttt tagaaaacct
     4801 taaaaaatgg cgtgagtggg aagtgccagt tattattgat gattggtgca atcgtatcct
     4861 aaacgaggat gaaacggaaa tactatactt tcctactcat gaggataaga tgaactatat
     4921 tagggtggaa aaagatttag aacctttgta tcatacatcg aacaagatct atgcaactat
     4981 ttctaaaagt gagtggttag aattgttaaa ttgatgattt aggaggtagc aatgacaaag
     5041 aagaaaattg agcgtatttc tgtaatacac cgagaaaaga ttttatggct caagtggtat
     5101 ttcatgcgag ataaagaaca acctaagtat agtgtccttg agcgtaaaat gtttgatgct
     5161 gctaaaaatc aagatatgct agcttatcaa aaatacgcaa ctatcaagca gataacagat
     5221 attagggtac aaacaagtga ggctgacatt ttagaggctg taaaagaggt ttatgtgtac
     5281 aatcacatga atgttatcgg agcttgtcag cggatattat ttatcagtca atcaccagct
     5341 tatgataagt taaataagtg gtttaatatc tattctgatt tgtattttag cgttgtaccc
     5401 ttgcccaaaa tgggggtata tcatgagatg gtaggtatct agttgattgt gtggtataat
     5461 attctaaagc acaagggata gtatagaagg agtacacctt gatagaggta gccacggtgc
     5521 gaatccgtgt cattgtgcta gcatctagga aactaggtgc tttttatttt aataacttaa
     5581 aagtatttac aaacaataac ttttaggtta taattgttat taggaagagg tgttaatagt
     5641 gcataatatc tatttttaca aggataaaaa cggcaatgag cctgtttttg attatatgcg
     5701 agagcttacc agtaaaaaag ggaaagatag cagaattaag cttaataaaa ttaatgatta
     5761 tattgagttg ttaagccaac atggaacacg cgcaggcgaa ccatatatta agcatttaga
     5821 tgctgaaatt tgggagctga gaccacttag agatagaatt ttatttgttg cttggatgga
     5881 tggtagtttt gttttactgc atcattttat gaaaaggaca cagaaaacac ccaaaagaga
     5941 gattgagcaa gctaaacgtg aacttgcaga tttaaaagaa agaggtttag acaatgaaaa
     6001 ataatgctat tggtagtaac tggaaagatg taagagctga attattcagc aaagaggaaa
     6061 ttttggaaag tgatatgcgc gtggctatca tgagtgagct tatcgaggct agaaatgaaa
     6121 agggtattag tcagaagaaa ctagaggaga tgagcggtgt tagtcagcca gttatcgcta
     6181 gaatggaaac aggaaagaca agcccacaac ttgatacagt attaaaagtg ttggcaagtc
     6241 ttggtaagac cttagctgtt gtaccattag agcatgagca ggtttagaaa gtttaggtga
     6301 tattttgaca gcagaaattg gaatattaa
//
LOCUS       DAVHEX010000087         5955 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVHEX010000087 DAVHEX010000000
VERSION     DAVHEX010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5955
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.088"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            6..230
                     /locus_tag="VVL45_002008"
                     /pseudo
     CDS             6..230
                     /locus_tag="VVL45_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(364..1332)
                     /locus_tag="VVL45_002009"
     CDS             complement(364..1332)
                     /locus_tag="VVL45_002009"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEU4188133.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(1477..2100)
                     /locus_tag="VVL45_002010"
     CDS             complement(1477..2100)
                     /locus_tag="VVL45_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEU4188134.1"
                     /translation="MPKKVLILSLLGGMFLSGWLSSFANTYIHDLLGVLFPDSPFLNA
                     FESAIAAPLVEEPLKLLSLVFVLALIPVRKLKSLFLLGIASGLGFQMIKDIGYIRTDL
                     PEGFDFTISRILERIISGIASHWTFSGLAVVGVYLLYRAYKGQKVGKKQGLIFLGLAL
                     GTHFLFNSPFVELETELPLAIPVVTAIALYGFYHAYCFVEKHNELMT"
     gene            complement(2316..2813)
                     /locus_tag="VVL45_002011"
     CDS             complement(2316..2813)
                     /locus_tag="VVL45_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEU4188135.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            complement(2886..4247)
                     /gene="radA"
                     /locus_tag="VVL45_002012"
     CDS             complement(2886..4247)
                     /gene="radA"
                     /locus_tag="VVL45_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEU4188136.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGFWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            complement(4261..4776)
                     /locus_tag="VVL45_002013"
     CDS             complement(4261..4776)
                     /locus_tag="VVL45_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU4188137.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(4778..5221)
                     /locus_tag="VVL45_002014"
     CDS             complement(4778..5221)
                     /locus_tag="VVL45_002014"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEU4188138.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            complement(5278..5375)
                     /gene="ffs"
                     /locus_tag="VVL45_002015"
     ncRNA           complement(5278..5375)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VVL45_002015"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5408..5875)
                     /locus_tag="VVL45_002016"
     CDS             complement(5408..5875)
                     /locus_tag="VVL45_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEU4188139.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYNILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
BASE COUNT         1843 a         1491 c         1001 g         1620 t
ORIGIN      
        1 tccagatttt agacatcatc aataaggata cacacaagga aatcatcgcc aaactggact
       61 acgacgcccc atcttgccct gagtgcggaa accaattgaa gaaatatgac tttcaaaaac
      121 cttctaaaat tccttatctt gaaacgactg gtatgcctac aagaattctc cttagaaagc
      181 gtcgattcaa gtgctatcac tgttcaaaaa tgatggtcgc tgaaacttct gatgacgtac
      241 agtcatattt cttctctttt tattatatca cagttttaaa tctagcttta ctagattcac
      301 cgctactatc tatttattcg gaaaaaagac gaaaaaacct gagaatcatc tcaggcttgg
      361 tcattaaatt tttttctcaa tatcgaaaag tggagaaagt ggtcgttttt catgaatacg
      421 tacgatagca tcccctagga gatgagcgat tgaaatctgc tcaatcttat caatcaaacg
      481 ctcttctggc agatagatgg tatccaaaac aaccaatttc ttaatagctg atttttggat
      541 attgtccgta gcaggaccag aaagaactgg gtgcgtacag cttgcataga cttcaacagc
      601 accagcttcc gcaagagcat ctgccgcatg acaaatcgtt ccagcggtat caatcatatc
      661 atcaatcaag atacaagtct tgccttcaac cttaccgatg atattcataa cttcactagt
      721 attcatctta tcaacgctac gacgtttatc aataatagcg atagatgttt tcaaaaattc
      781 tgccaactta cgagcacgag tcacccctcc atggtccggg ctgacaacca catagtcaga
      841 accaaccata ccacgacgct caaaataatc tgcaatcaga ggagcaccca tcaaatgatc
      901 cacaggaata tcaaagaatc cttgaatttg cgcagcatgc aagtcgatgg tcaataaacg
      961 atccactcca gctacttcaa gcatatttgc gacaagtttt gaagtgattg gctcacgcgc
     1021 tctcgccttt ctatcctgac gtgcataccc atagtaaggc atgacaacat tgacagattc
     1081 tgcactcgca cgcttcaaag catctaccat aatcaaaatt tcaagcagat tgtcatttac
     1141 aggcgaacta gttgattgta agataaagac gtgtttccca cggattgatt cttcaatgtt
     1201 gacctgaatc tctccatctg aaaattggcg aacacttgat ttccccaact ctatcccaat
     1261 ctcctgcgcc acacgttctg ccaattcttt attagaagaa agggcaaaca gctttaaatc
     1321 agaaaaagac atgatttcct ccggtatata tgtataactt gtgcttttca caagattttc
     1381 catctaccat tgtagcgctt tttgcactat ttttcaatca aaaataaaag aagggcacca
     1441 tatttgtacc cttgcatcat tcttttgaaa aatattctag gtcatcaact cattgtgttt
     1501 ctcaacaaag caataagcat gataaaaacc atagagagca atagccgtaa ccactggaat
     1561 cgctaaaggc aactctgttt ccaactccac aaaaggagag ttaaacaaga agtgagttcc
     1621 caaggctaaa cctagaaaaa taaggccctg tttcttgcca accttctgtc ctttataggc
     1681 tctgtaaagc aagtaaacac ctactacagc tagacctgaa aaagtccagt gagaggcaat
     1741 tcctgagatg atacgctcta aaattcgcga aatagtaaag tcaaagccct ctggcaaatc
     1801 cgtacgaata taaccaatat ccttaatcat ttggaatccc aaaccggaag caattccaag
     1861 taaaaacaaa gattttaatt ttcgcacagg aatcaaagcc aaaacaaaaa caagtgacaa
     1921 taatttcaag ggttcttcta ccaaaggagc cgcaatagca ctttcaaagg catttaaaaa
     1981 tggactatct gggaaaagaa cccccagtaa atcatggata taagtattag caaaactaga
     2041 caaccagcct gaaaggaaca tccctcccaa taaagacaga atcaaaacct tctttggcaa
     2101 ttcccatttt tcccaatacg gaagagaaaa taaagagccg gaatcatgta aaagagagct
     2161 agaaagatag aaactcccat tagtccatat tccgcacctg acctcgaacc gtccgtatag
     2221 tagatggttt catactgtaa accaatacat agcaataaaa taaaaataaa taaaatactg
     2281 cttttcttca tacactttct ttctaaatga agtatttata attctacgac tgtcatactt
     2341 cctgtatcaa cattgtaaat ggcaccagag ataatgacat cgtctggtat taggggagac
     2401 tcgataagca gttgcatatc ctcgcgtaca ctctcttcta tatcttggaa gggcaagaag
     2461 tcctggtctg acacatcgac acccaattct tccttcaaat actcctgaaa aggttcattt
     2521 tcaaaggtct gagcaccaca gtctgtatga tgcaatacca caatttctct tgtccccatt
     2581 tgttgctggg aaataactag agaacgaatc atatcctcag tcactcgacc acctgcattc
     2641 cgcaaaatat gagcatcccc aagtgccaaa cctagagctt gcgcaacgtg caaacgtgag
     2701 tccatacagg tcacaatggc tactctggtt ttaggtttaa gtggcagatt taactgccca
     2761 tgtagggcaa cataagcctg attggcttgc ataaactgtt caaaatacga cacgattccc
     2821 tccttgaaaa tttgatagtc aaatatttct cctatcttat catttttaag agaatttgtc
     2881 acggattatg caaagacctt tttcaagact tcctgaatcg ttgtcacgcc aatgacctga
     2941 atttccttag gcagagtgat tcctgtcaag gaattcttag gtacataaat cttagtaaag
     3001 cccagtttag cagcttcgtt gatgcgttgc tcaatacgat tcacgcgccg aatctctcct
     3061 gtcaagccca gttctccgac aaaacattcc tgaggattag ttggcttgtc tttgtagctc
     3121 gaagcaatag caactgcaac agccaagtca atcgcaggtt catccaattt aacaccacca
     3181 gcagatttga gataggcatc ctgattttgc aagagaagcc ctgcccgttt ttccaaaaca
     3241 gccataatca agctagcacg gttaaaatca agtcctgtcg tagtacgctt ggcatttcca
     3301 aacatggtcg gtgttaccaa agcctgaacc tccgccaaaa tcggacgcgt cccttccatg
     3361 gttacaacga tggaggaacc agtcgcccca tccaaacgct cttctaggaa aacttgactc
     3421 ggattgagta cctcaaccaa gccgcccgac tgcatctcaa aaatcccaat ctcattagtg
     3481 gaaccaaaac gatttttgac cgctctcaaa atacgaaagg tgtggtgacg ctccccttca
     3541 aagtaaagca ccgtatccac catatgctcc aacatacgag gcccagccaa ggttccttct
     3601 ttggtcacat gacctacgat aaagatggca atgttattgg tcttggccaa ctgcatgagt
     3661 tcagcggtca cttcacgcac ctgagaaaca gacccctgca cccctgaaat ctcaggagac
     3721 atgatggtct ggatggaatc aataatgaga aagtctggct ggatacgctc cacttctgca
     3781 cgaacactct gcatattggt ctctgcatag agataaaact cactatcaat atcacctaag
     3841 cgctctgcac gtagtttaat ctgctgggca gactcctccc cactgacata gagaactgtc
     3901 cccacttggg acaactgggt tgagacttgt aggagaagag ttgatttccc aatcccagga
     3961 tccccaccga taaggacgag acttcctggt accactccgc ctccaagcac acggttgaat
     4021 tcctccatct ccgtcttggt tcgattgaca ttgatggaag tcacctcagc tagtttcatg
     4081 ggcttggttt tctcacctgt caaggacaca cgcgcattct taacttcggc aacctcaacc
     4141 tcttccacaa aagaagacca aaacccacag ttggggcaac gtcccagata tttaggggaa
     4201 ttatacccac aattttgaca tacaaatgtc gcttttttct ttgcgatgac aaacctcttt
     4261 ctatatctct aactcacact caatcacttg gcaaaaatca atcttctcat ttggcacaaa
     4321 ctggcgcatg agcattcgat gagcaacaac taccacagtc tgatgttctc gatacttaga
     4381 catacattct agaaaccgag acttcatttc cgtagctgtc tcatattgaa taggactatt
     4441 aggaagcaac tcccccttgt tttctaaaaa cagtcttcta gctgtttcaa agttttctat
     4501 tcctgtttta tagacctgcc attcatgtaa taaaggctct actcttaaag gaagacccgt
     4561 agcacagacc acatacgaag ccgtttctaa agctcttgtg actgcagaag atacgattat
     4621 ttcagctgac gagagtaaag gatttttgct caatttctgg acttgctgcc gtcccatctc
     4681 agacaagggt gccaaatcta tcccaaatcc tatataagaa cgctcctcta actcacggta
     4741 atctggctcc ccatgacgta caaagataat cttcattcta gtgccctgtc gatccaaatc
     4801 caccagttcg aacgccatca gctgcatctc catctgcaat taagaaagta gcaaaaacag
     4861 cctggacaat acgctcccca acttcaagaa caacctcttg gtctgtgata ttcttcatct
     4921 gcgcaaaaat atgcccttca tttccaggat ttccataata atccccatca atgactccaa
     4981 ctgagttaat taaaaccaag cccttcttac gaggatttga agaacgatca tagaggtaga
     5041 gaacctcagt cggctgcata taagccttaa cccctgtcgg aaccaagaca atctctcctg
     5101 gcgcaacaac tgtacgcaca gcaaccttta agtcgtaacc agccgcatgc gctgtctcac
     5161 gcttgggcaa taaattttca tctgtaaaac tcgaaaccaa ttcaaaacca cgaattttca
     5221 taattttctc ttttctatta tcatttattc tagattattc tatcttattt attcggaaaa
     5281 agcacgaaaa aaagagcaca caattcaaat cgcttagggc tgctggattc ctcccctgac
     5341 ccgcttcacg cagaactgtt gctccactat ttattatatc acattcctat tcattttaaa
     5401 agcaaaatta ttttttccgt ctatttctaa aaaaatcctg catgatagct gcgcattcat
     5461 cttccaaaat tcctatttca acctccacac gatgattgag acgctcatct gtcaagatat
     5521 tgtacaaact cccagcagcg ccaaatttct ggtttttagc cccatagacc acatttggaa
     5581 tgcgggcaag cccaatcgct ccactacaca tgacacaagg ttcaatggtc acaaaaagtg
     5641 tgcaatccag caagcgccag ctctcctcac tcaagttcgc atcctctata gccataattt
     5701 ccgcatgcat aaccgctcgc tgtaattcct cacgcgcatt atgcccacga ccaatgattt
     5761 ccccatcttt gacaatcaca caaccaattg gaatttcatc gtgttcaaga gcaatctcag
     5821 cctctctcaa agcctccctc ataaagactt ctttttcttc aagcgtataa tacatctctt
     5881 ttctcttttc ctacttatcg attttattat tatatcatga atccaacaca aaaaaagcca
     5941 ccgaatgcgg tgact
//
LOCUS       DAVHEX010000088         5905 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVHEX010000088 DAVHEX010000000
VERSION     DAVHEX010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5905
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.089"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(184..1194)
                     /gene="ccpA"
                     /locus_tag="VVL45_002017"
     CDS             complement(184..1194)
                     /gene="ccpA"
                     /locus_tag="VVL45_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEU4188140.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(1619..2218)
                     /locus_tag="VVL45_002018"
     CDS             complement(1619..2218)
                     /locus_tag="VVL45_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU4188141.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLMLQPDVESVFQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRAEKLETKVVVVTTFKRPGYFERAVKAGVDAYVL
                     KERNIADLMQTLHTVLEGRKEYSPELMEVVMMHPNPLTEQEIAVLKGIAQGFSNQEIA
                     DKLYLSNGTVRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(2219..3316)
                     /locus_tag="VVL45_002019"
     CDS             complement(2219..3316)
                     /locus_tag="VVL45_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU4188142.1"
                     /translation="MLERLKRIHYMFWISLIFMIFPILSVVTGWLSAWHLLIDILFVV
                     AYLGVLTTKSQRLSWLYWGLMLTYVVGNTAFVAVNYIWFFFFLSNLLSYHFSVRSLKS
                     LHVWTFLLAQVLVVGQLLIFQRIEVEFLFYLLVILTFVDLMTFGLVRIRIVEDLKEAQ
                     VKQNAQINLLLAENERSRIGQDLHDSLGHTFAMLSVKTDLALQLFQMEAYPQVEKELK
                     EIHQISKDSMNEVRTIVENLKSRTLTSELETVKKMLEIAGIEVQVENHLDKSSLTQEL
                     ESTASMILLELVTNIIKHAKASKVYLKLERTEKELILTVRDDGCGFASISGDELHTVR
                     NRVFPFSGEVSVISQKHPTEVQVRLLYKERK"
     gene            complement(3316..4053)
                     /locus_tag="VVL45_002020"
     CDS             complement(3316..4053)
                     /locus_tag="VVL45_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4188143.1"
                     /translation="MKRWLALSKIEFLLTKRQLIYYLLSVGMPTAFYLFFSGIYQDTP
                     GELANFMRDYLISMTAFSMMSTAIFSFPVVLHTDKINNWQKTLRHSPVNMVEYYLSKI
                     TSMLVDYLVSILVVFSVGHFVRGVDMSLGNWIGAALLLIVGSIAFVALGLTLTLLPTS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQVVGKCLPTYQLMELLKTFLNEGGINL
                     SATVYLLVFSVVLFGLTIYLQGHKENA"
     gene            complement(4055..4939)
                     /locus_tag="VVL45_002021"
     CDS             complement(4055..4939)
                     /locus_tag="VVL45_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4188144.1"
                     /translation="MNMIKVESLNKNIKGKAILKGISFEVAEGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLSVTSSQLDYIRGYLPQENVIVQKLKVKELIAFFQRIY
                     PNSLSDQEIDQLLQFDQQQKEQFAEKLSGGQKRLFSFVLTLIGRPKLVFLDEPTAAMD
                     TSTRQRFWEIVRDLKAQGVTILYSSHYIEEVEHTADRILVLNKGELIRDTTPLAMRSE
                     GIEKHFILPLAYKEVIEQSNLVENWSQKQDALQVVTREADAFWELLVQAGCGIQEIEV
                     NNRSLLDTIFEETQKGDN"
     gene            complement(4926..5120)
                     /locus_tag="VVL45_002022"
     CDS             complement(4926..5120)
                     /locus_tag="VVL45_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188145.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMFTGWFDQTLLRQFIRGALGFGFM
                     IFVVFLMRMEWLKGEYHEYD"
     gene            complement(5117..5248)
                     /locus_tag="VVL45_002023"
     CDS             complement(5117..5248)
                     /locus_tag="VVL45_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine aminotransferase"
                     /protein_id="HEU4188146.1"
                     /translation="MFSGLDESFYHFPWELFAGFGMMSWLVREGLKLVGDVKKELEE"
BASE COUNT         1887 a         1327 c          956 g         1735 t
ORIGIN      
        1 agttctatct gcaacctcaa agcagtgctt tgagctgact tcgtcagttc tatctacaac
       61 ctcaaaacag tgtgttgcgc agcctttaat cagccgccta gtccgctcta tggtattcat
      121 taagtcaaca tctcttgttt aagagcacca aatcaggaaa tcttctcgat tccctgattt
      181 tttctattta cgttttcgtg ttgagctacg ttctgtcaaa ccatgaggta agagaacttc
      241 acgttcttcc aactcttcct tatgcataat cttggtcaac atacgcatac taatggcacc
      301 aaggtcataa agaggttggg caatcgttgt caagtttgga cgggtaaagc gtgagatttg
      361 tgaatcatca ctagtaataa tttcaaaatc ttctggcaca gaaacaccct tatcagccaa
      421 accgttcaag actcctgctg ccaactcatc acctgtcaca actgctgcag ttgcatttga
      481 tgaaatcaaa cgctctgcta aggcgtaacc atcatcatag ctatatttag attcaaatac
      541 caaaccctca ctataagtga ttcctgcttt tttcaaggtt tccttgtagc caactaaacg
      601 aaccttacca ttgatgtcat ccactagcgg accgctaacg aaagcaatac gctcattttc
      661 tttagcaagg taactcactg catcaattgt tgcttgctta tagtcaatat tgacacttgg
      721 caactggtgc tcaacatcga cagttcctgc gagaacaatc ggagtacgtg aacgcgaaaa
      781 ttctgagcga attttatctg tcaagtgata ccccatatag ataatgccat ctacctgctt
      841 tgaaaagagg gtattgacaa cagaaacttc tttctcgtta tcttcatcgc tattagctag
      901 gacaatattg tacttgtaca tttctgcaat atcatcaatc cccttagcca aactcgaaaa
      961 ataaccattg gtaatatttg gaatcacgac accgacagtg gttgtctttt tacttgcaag
     1021 accacgcgca actgcatttg gacgataatc caaacgatca attacctcta gcactttttt
     1081 acgggtattc tcttttacat ttttattgcc attgaccaca cggctgaccg tcgccatgga
     1141 aacacctgct tcacgagcga catcataaat ggttactgta tcatctgcat tcattccttt
     1201 tcctgtcctt tctatctcac acattctttt acaagtagag gtactgattg aagctctata
     1261 tctacttaca aaagtgaaga tgtgaaaatt tcgttttcat atttctactt attccattct
     1321 atcactaatt gtaaacactt tcaagtgttt tttgaagatt gattgaaaaa atttcataga
     1381 aaacctaggt ttagctcctt gctaccacct tagactaaac aaaaaggagg aaactaagcc
     1441 ctcctaaagt tatagtaaaa tgaaataaga acaggataaa tcgatcagga cagtcaaatc
     1501 gatttctaac aatgttttag aagtagaggt gtactattct agtttcaatc tactataggt
     1561 attgttccat tcactaccgt caattttagc acatagtctt catgaaaata ttatatcatc
     1621 ataaccaacc agattctttc gcgatattag ctgcctctgt tcgattacct gcatctagtt
     1681 tcgaaagaat attggtgaca tagtttcgga ctgttccgtt ggatagataa agtttgtctg
     1741 caatttcttg gttagagaag ccctgagcaa ttccctttaa aactgcgatt tcttgctccg
     1801 ttaatggatt gggatgcatc atcaccactt ccatcaattc aggcgaatac tccttgcgtc
     1861 cttcgaggac ggtgtgcaag gtttgcatga ggtctgcaat gtttctttct tttaatacat
     1921 aagcatctac tccagccttg accgcacgtt caaaataccc aggacgcttg aaggtcgtca
     1981 ccacaaccac ctttgtttca agcttttctg ctcgtatcca ctccaagact tcaagacctg
     2041 tcttaacagg catttctacg tcaaggatgg cgatatctac agactccttt tctaatagtt
     2101 ggattgcttc ttgcccattc ttggcttgaa agacagactc tacatccggt tgaagcatga
     2161 gcaactggca catggcatct cgcaacatac tttgatcttc tgcgactaat actttcatct
     2221 actttctctc cttataaagt agtcgaacct gcacttcagt tggatgtttc tgactgatta
     2281 cacttacttc tcctgaaaat ggaaaaacac gatttcggac tgtatggagc tcatccccgc
     2341 ttatagaggc aaagccacag ccatcatctc tcactgttag aatgagttct ttctctgtcc
     2401 gttctaattt caagtagact ttagacgctt tagcatgttt gatgatattg gtcactaatt
     2461 caagcaaaat catggaagcc gttgactcca attcctgagt taagctagac ttgtccaagt
     2521 gattctcaac ttgaacctca attccagcaa tttctaacat ctttttcaca gtctctagtt
     2581 cggatgtcaa agttctagac ttaagatttt ccacaatggt tcgcacttca ttcatggaat
     2641 ccttgctgat ctggtgaatt tcttttaatt ccttttccac ctgtggataa gcctccatct
     2701 gaaataactg caaggctaaa tctgtcttga cactcagcat agcaaaggta tgtcccagac
     2761 tatcatgcaa atcctgaccg atacgactac gttcattttc agcaagcaat agatttatct
     2821 gagcattttg cttgacctga gcttctttca aatcctcgac aatacgaatc cgaaccaatc
     2881 caaaagtcat taaatcgaca aaagtaagaa ttacaagtag atagaataga aactcaactt
     2941 cgattctctg aaaaatcaac agttgcccca caacaaggac ttgagcaaga agaaaagtcc
     3001 agacatgtaa agactttaaa ctacgtacgc tgaaatgata acttaagaga ttggatagga
     3061 aaaagaaaaa ccagatataa ttaacagcaa caaaggcagt attcccaact acataagtca
     3121 gcatgaggcc ccaatatagc caagataggc gctggctctt agttgttaaa acacccaaat
     3181 atgccactac aaatagaata tcaatcaata aatgccaggc agaaagccac ccagtcacta
     3241 cagacaggat ggggaaaatc ataaaaatta aactgatcca aaacatataa tgtattcttt
     3301 tcagtctttc aagcattaag cattctcctt atgaccttga aggtaaatgg tcaaaccaaa
     3361 caaaactact gaaaaaacaa gtaaataaac tgtggctgat agattgatgc caccctcatt
     3421 taagaaggtc ttgagcaact ccatcaactg ataggtcggg agacacttac ctactacttg
     3481 catccagtct ggaaataaag agataggcat ccagagtcca cctaaaacag ccaaccctag
     3541 ataaagaaga ttgcccacga cagacatcaa ctgactagtt ggtaagagag tcaaggtcaa
     3601 accaagcgct acgaaggcaa tacttcctac tatcagcaaa agtgcagccc caatccaatt
     3661 tccaagagac atgtccacac ctcttacaaa atgcccaact gagaaaacca ccaagattga
     3721 aaccaaataa tcaaccagca tacttgttat ctttgataga taatattcta ccatatttac
     3781 agggctatga cgcaatgttt tctgccagtt gttgatcttg tcggtatgta aaacaactgg
     3841 gaatgagaag atagctgttg acatcatgga aaatgcagtc atggagataa gataatcacg
     3901 cataaaattc gcgagttcac ctggtgtgtc ctgatagata ccagaaaaaa ataaatagaa
     3961 agccgtcggc atccctactg acaatagata atagatcaat tgtcgtttgg tcaataaaaa
     4021 ttctatctta ctaagtgcta gccatcgttt catcttagtt atctcccttc tgcgtttctt
     4081 caaagattgt atccaacaaa ctacgattat taacttcaat ttcttgtatg ccacatcctg
     4141 cttgaactaa cagttcccaa aaagcatctg cttcgcgtgt gactacttgt agagcatcct
     4201 gtttttgtga ccagttttca accaagttag actgctcaat gacttccttg tatgccagag
     4261 gaaggataaa atgcttttca attccctcac tacgcatagc tagaggcgtc gtatcacgaa
     4321 tcaactctcc cttatttaaa accaaaatcc ggtcagccgt atgctctacc tcttcaatat
     4381 aatgagacga atagagaatc gtgactcctt gcgcttttag gtcccgaacg atttcccaaa
     4441 agcgttgacg agttgaagta tccatggcag cagttggttc atctaaaaag acaagctttg
     4501 gtcgcccaat caaggtcaag acaaaagaga agagacgctt ttgcccgcct gacaattttt
     4561 ctgcgaattg ctctttttgt tgctggtcaa actgcaatag ttgatcgatt tcctgatcgc
     4621 tcaaggaatt tggatagata cgttgaaaga aagcaatcaa ctctttgacc tttaatttct
     4681 gaacgatgac attttcttga ggcagataac ctctaatata gtctaactga gaactcgtca
     4741 ctgacaagcc ttggatggat acttgaccgc ttgtgaccag tttatctcca agcagacagt
     4801 ccaagagtgt ggtcttccca gcaccattgg gcccaatcaa ggcgacgcat tcaccttcag
     4861 ctacctcaaa ggaaataccc ttcaaaatag ccttgccctt gatgttttta tttaggcttt
     4921 ctaccttaat catattcatg atattctcct ttcaaccact ccattctcat aaggaaaacg
     4981 acgaaaatca taaatccaaa ccccaaagca ccacgaatga attggcgaag caaggtttgg
     5041 tcaaaccaac ctgtaaacat ttccactaac cataccaaga gtgacaggcc gataaagaaa
     5101 tagatgatcc ctctcttcat tcctcaagct cctttttcac atctccgact aatttcaaac
     5161 cttctctaac aagccaagac atcattccaa agccagcaaa gagctcccaa ggaaaatgat
     5221 agaaactctc atccaatccc gaaaacatga gttaggtcat aactcctgct actactaaac
     5281 tcactgcgat aatcatttta tttctcatct cttcttcctc catttcatac tacaattata
     5341 gtcttttgaa atcagaggag acagaagctt ctgtcactag aaaatatgac aaatgtcata
     5401 aaaaattctg ttcaaaacaa gcaagataca ctatacaata aaacacaatt agaaaaatct
     5461 aaggcaactt cctcaaaaga gatatcaaac ccaattcaca ccataatgta aactaatact
     5521 tatttaaaat caaaaagagt agaaattttt atcagacaaa cacatatata gtgtattgaa
     5581 tctataacag taggccttaa atactaaaat atttctataa attaatttaa ctttcctgat
     5641 agagctgttc atatcttatt tcaattctct aaattatacg ttgaacaaaa cccttctatt
     5701 tctttcttaa agatttataa gagttataaa atctgttaaa tttcaatgtg tatacctaaa
     5761 ctacggtatt tattgaaaag actggagaca aaaagtatac gctgccaaaa tgaattactg
     5821 aaaatcaaaa aagagagaac caaactgatt ccctcttaat gtatataata tctagtttta
     5881 aaaatacaca ctcacatatc tctgt
//
LOCUS       DAVHEX010000089         5835 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVHEX010000089 DAVHEX010000000
VERSION     DAVHEX010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5835
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.090"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            7..273
                     /locus_tag="VVL45_002024"
     CDS             7..273
                     /locus_tag="VVL45_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEU4188147.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            266..634
                     /locus_tag="VVL45_002025"
     CDS             266..634
                     /locus_tag="VVL45_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEU4188148.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            754..2022
                     /locus_tag="VVL45_002026"
     CDS             754..2022
                     /locus_tag="VVL45_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEU4188149.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKVWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            2019..3296
                     /gene="tilS"
                     /locus_tag="VVL45_002027"
     CDS             2019..3296
                     /gene="tilS"
                     /locus_tag="VVL45_002027"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEU4188150.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETIFMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVEDLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEKEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ELIQQIPVSRETSIHIRHRKTGDVLIKNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            3300..3842
                     /gene="hpt"
                     /locus_tag="VVL45_002028"
     CDS             3300..3842
                     /gene="hpt"
                     /locus_tag="VVL45_002028"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEU4188151.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKEREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            3858..5816
                     /gene="ftsH"
                     /locus_tag="VVL45_002029"
     CDS             3858..5816
                     /gene="ftsH"
                     /locus_tag="VVL45_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEU4188152.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEETGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMLGAQSPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDEK"
BASE COUNT         2036 a          869 c         1195 g         1735 t
ORIGIN      
        1 ataaggatga gattagataa atatttaaaa gtatcgcgaa ttatcaagcg tcgtacagtc
       61 gcaaaggaag tagcagataa aggtagaatc aaggttaatg gaatcttggc caaaagttca
      121 acggacttga aagttaatga ccaagttgaa attcgctttg gcaataagtt gctgcttgta
      181 aaagtactag agatgaaaga tagtacaaaa aaagaagatg cagcaggaat gtatgaaatt
      241 atcagtgaaa cacgggtaga agaaaatgtc taaaaatatt gtacaattga ataattcttt
      301 tattcaaaat gaataccaac gtcgtcgcta cctgatgaaa gaacgacaaa aacggaatcg
      361 ttttatggga ggggtattga ttttgattat gctattattt atcttgccaa cttttaattt
      421 agcgcagagt tatcagcaat tactccaaag acgtcagcaa ttagcagact tgcaaactca
      481 gtatcaaact ttgagtgatg aaaaggataa ggagacagca tttgctacca agttgaaaga
      541 tgaagattat gctgctaaat atacacgagc gaagtactat tattctaagt cgagggaaaa
      601 agtttatacg attcctgact tgcttcaaag gtgataaaat ggaaaattta ttagacgtaa
      661 tagagcaatt tttgagtttg tcagatgaaa agctggaaga attggctgat aaaaatcaat
      721 tattgcgttt acaagaagaa aaggaaagga agaatgcgta aattcttaat tattttgttg
      781 ctaccaagtt ttttgaccat ttcaaaagtc gttagcacag aaaaagaagt cgtctatact
      841 tcgaaagaaa tttattacct ttcacaatct gactttggta tttattttag agaaaaatta
      901 agttctccca tggtttatgg agaggttcct gtttatgcga atgaagattt agtagtggaa
      961 tctgggaaat tgactcccaa aacaagtttt caaataaccg agtggcgctt aaataaacaa
     1021 ggaattccag tatttaagct atcaaatcat caatttatag ctgcggacaa acgattttta
     1081 tatgatcaat cagaggtaac tccaacaata aaaaaagtat ggttagaatc tgactttaaa
     1141 ctgtacaata gtccttatga tttaaaagaa gtgaaatcat ccttatcagc ttattcgcaa
     1201 gtatcaatcg acaagaccat gtttgtagaa ggaagagaat ttctacatat tgatcaggct
     1261 ggatgggtag ctaaagaatc aacttctgaa gaagataatc ggatgagtaa agttcaagaa
     1321 atgttatctg aaaaatatca gaaagattct ttctctattt atgttaagca actgactact
     1381 ggaaaagaag ctggtatcaa tcaagatgaa aagatgtatg cagccagcgt tttgaaactc
     1441 tcttatctct attatacgca agaaaaaata aatgagggtc tttatcagtt agatacgact
     1501 gtaaaatacg tatctgcagt caatgatttt ccaggttctt ataaaccaga gggaagtggt
     1561 agtcttccta aaaaagaaga taataaagaa tattctttaa aggatttaat tacgaaagta
     1621 tcaaaagaat ctgataatgt agctcataat ctattgggat attacatttc aaaccaatct
     1681 gatgccacat tcaaatccaa gatgtctgcc attatgggag atgattggga tccaaaagaa
     1741 aaattgattt cttctaagat ggccgggaag tttatggaag ctatttataa tcaaaatgga
     1801 tttgtgctag agtctttgac taaaacagat tttgatagtc agcgaattgc caaaggtgtt
     1861 tctgttaaag tagctcataa aattggagat gcggatgaat ttaagcatga tacgggtgtt
     1921 gtctatgcag attctccatt tattctttct attttcacta agaattctga ttatgatacg
     1981 atttctaaga tagccaagga tgtttatgag gttctaaaat gagggaacca gattttttaa
     2041 atcattttct caagaaggga tatttcaaaa agcatgctaa ggcggttcta gctctttctg
     2101 gtggattaga ttccatgttt ctatttaagg tattgtctac ttatcaaaaa gagttagaga
     2161 ttgaattgat tctagctcat gtgaatcata agcagagaat tgaatcagat tgggaagaaa
     2221 aggaattaag gaagttggct gctgaagcag agcttcctat ttatatcagc aatttttcag
     2281 gagaattttc agaagcgcgt gcacgaaatt ttcgttatga tttttttcaa gaggtcatga
     2341 aaaagacagg tgcgacagct ttagtcactg cccaccatgc tgatgatcag gtggaaacga
     2401 tttttatgcg cttgattcga ggaactcgct tgcgctatct atcaggaatt aaggagaagc
     2461 aagtagtcgg agagatagaa atcattcgtc ccttcttgca ttttcagaaa aaagactttc
     2521 catcaatttt tcactttgaa gatacatcaa atcaggagaa tcattatttt cgaaatcgta
     2581 ttcgaaattc ttacttacca gaattggaaa aagaaaatcc tcgatttagg gatgcaatct
     2641 taggcattgg caatgaaatt ttagattatg atttggcaat agctgaatta tctaacaata
     2701 ttaatgtgga agatttacag cagttatttt cttactctga gtctacacaa agagttttac
     2761 ttcaaactta tctgaatcgt tttccagatt tgaatcttac aaaagctcag tttgctgaag
     2821 ttcagcagat tttaaaatct aaaagccagt atcgtcatcc gattaaaaat ggctatgaat
     2881 tgataaaaga gtaccaacag tttcagattt gtaaaatcag tccgcaggct gatgaaaagg
     2941 aagatgaact tgtgttacac tatcaaaatc aggtagctta tcaaggatat ttattttctt
     3001 ttggacttcc attagaaggt gaattaattc aacaaatacc tgtttcacgt gaaacatcca
     3061 tacacattcg tcatcgaaaa acaggagatg ttttgattaa aaatgggcat agaaaaaaac
     3121 tcagacgttt atttattgat ttgaaaatcc ctatggaaaa gagaaactct gctcttatta
     3181 ttgagcaatt tggtgaaatt gtctcaattt tgggaattgc gaccaataat ttgagtaaaa
     3241 aaacgaaaaa tgatataatg aacactgtac tttatataga aaaaatagat aggtaaaaaa
     3301 tgttagaaaa cgatattaaa aaagtcctcg tttcacacga tgaaattaca gaagcagcta
     3361 aaaaactagg tgctcaatta actaaagact atgcaggaaa aaatccaatc ttagttggga
     3421 ttttaaaagg atctattcct tttatggctg aattggtcaa acatattgat acacatattg
     3481 aaatggactt catgatggtt tctagctacc atggtggaac agcaagtagt ggtgttatca
     3541 atattaaaca agatgtgact caagatatca aaggaagaca tgttctattt gtagaagata
     3601 tcattgatac aggtcaaact ttgaagaatt tgcgagatat gtttaaagaa agagaagcag
     3661 cttctgttaa aattgcaacc ttgttggata aaccagaagg acgtgttgta gaaattgagg
     3721 cagactatac ttgctttact atcccaaatg agtttgtagt aggttatggt ttagactaca
     3781 aagaaaatta tcgtaatctt ccttatattg gagtattgaa agaggaagtg tattcaaatt
     3841 agaaagaata atctttaatg aaaaaacaaa ataatggttt aattaaaaat ccttttctat
     3901 ggttattatt tatctttttc cttgtgacag gattccagta tttctattct gggaataact
     3961 caggaggaag tcagcaaatc aactatactg agttggtaca agaaattacc gatggtaatg
     4021 taaaagaatt aacttaccaa ccaaatggta gtgttatcga agtttctggt gtctataaaa
     4081 atcctaaaac aagtaaagaa gaaacaggta ttcagttttt cacgccatct gttactaagg
     4141 tagagaaatt taccagcact attcttcctg cagatactac cgtatcagaa ttgcaaaaac
     4201 ttgctactga ccataaagca gaagtaactg ttaagcatga aagttcaagt ggtatatgga
     4261 ttaatctact cgtatccatt gtgccatttg gaattctatt cttcttccta ttctctatga
     4321 tgggaaatat gggaggaggc aatggccgta atccaatgag ttttggacgt agtaaggcta
     4381 aagcagcaaa taaagaagat attaaagtaa gattttcaga tgttgctgga gctgaggaag
     4441 aaaaacaaga actagttgaa gttgttgagt tcttaaaaga tccaaaacga ttcacaaaac
     4501 ttggagcccg tattccagca ggtgttcttt tggagggacc tccggggaca ggtaaaactt
     4561 tgcttgctaa ggcagtcgct ggagaagcag gtgttccatt ctttagtatc tcaggttctg
     4621 actttgtaga aatgtttgtc ggagttggag ctagtcgtgt tcgctctctt tttgaggatg
     4681 ccaaaaaagc agcaccagct atcatcttta tcgatgaaat tgatgctgtt ggacgtcaac
     4741 gtggagtcgg tctcggcgga ggtaatgacg aacgtgaaca aaccttgaac caacttttga
     4801 ttgagatgga tggttttgag ggaaatgaag ggattatcgt catcgctgcg acaaaccgtt
     4861 cagatgtact tgaccctgcc cttttgcgtc caggacgttt tgatagaaaa gtattggttg
     4921 gtcgtcctga tgttaaaggt cgtgaagcaa tcttgaaagt tcacgctaag aataagcctt
     4981 tagcagaaga tgttgatttg aaattagtgg ctcaacaaac tccaggcttt gttggtgctg
     5041 atttagagaa tgtcttgaat gaagcagctt tagttgctgc tcgtcgcaat aaatcgataa
     5101 ttgatgcttc agatattgat gaagcagaag atagagttat tgctggacct tctaagaaag
     5161 ataagacagt ttcacaaaaa gaacgagaat tggttgctta ccatgaggca ggacatacca
     5221 ttgttggtct agtcttgtcg aatgctcgcg ttgtccataa ggttacaatt gtaccacgcg
     5281 gccgtgcagg cggatacatg attgcacttc ctaaagagga tcaaatgctt ctatctaaag
     5341 aagatatgaa agagcaattg gctggcttaa tgggtggacg tgtagctgaa gaaattatct
     5401 ttaatgtcca aaccacagga gcttcaaacg actttgaaca agcgacacaa atggcacgtg
     5461 caatggttac agagtacggt atgagtgaaa aacttggccc agtacaatat gaaggaaacc
     5521 atgctatgct tggtgcacag agtcctcaaa aatcaatttc agaacaaaca gcttatgaaa
     5581 ttgatgaaga ggttcgttca ttattaaatg aggcacgaaa taaagctgct gaaattattc
     5641 agtcaaatcg tgaaactcac aagttaattg cagaagcatt attgaaatac gaaacattgg
     5701 atagtacaca aattaaagct ctttacgaaa caggaaagat gcctgaagca gtagaagagg
     5761 aatctcatgc actatcctat gatgaagtaa agtcaaaaat gaatgacgaa aaataaccct
     5821 gagagaggct ggagc
//
LOCUS       DAVHEX010000090         5406 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVHEX010000090 DAVHEX010000000
VERSION     DAVHEX010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5406
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.091"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            7..1014
                     /locus_tag="VVL45_002030"
     CDS             7..1014
                     /locus_tag="VVL45_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEU4188153.1"
                     /translation="MDTNLFSKINTTISVKENDFITEEKFQKIIQSKDTETLAFILES
                     TPYHLSIDILEDPSQTEISLMTKLVNDYRWAYAESPSDIIVTLFALRYVYHNIKVLLK
                     SKAAIKKDFSKLLIPIGIFDIESLKHLVSSLHSDTLPDFMVREVESIWNEYETFNNIR
                     VLDVGADLAYFKHLKLLSNELDEVLSQVIVEMIDFYNIITVKRGLSQNKSHGDILQLL
                     SDEGSISAKEFIYIVENQEIFVWFNKINPSLDSIFSTYELKMQDATISSSELEFLCDL
                     LLYKTLDQGRYNVEGPLVLARYLLGCEFEVKNLRMIISALQNTIPFESIKERIRPHYG
                     S"
     gene            1004..1324
                     /locus_tag="VVL45_002031"
     CDS             1004..1324
                     /locus_tag="VVL45_002031"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEU4188154.1"
                     /translation="MEANKYKIGIIGSRDIILPFSMIGFDIFPAYQEQEAINTLRKLA
                     QSDYGVIYITEDIASMILDTIRHYDSQVVPAIILLPTHKQGLNLGLKRIEDNVEKAVG
                     HNIL"
     gene            1387..3162
                     /locus_tag="VVL45_002032"
     CDS             1387..3162
                     /locus_tag="VVL45_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEU4188155.1"
                     /translation="MTQGKIIKVSGPLVIASGMQEANIQDICRVGKLGLIGEIIEMRR
                     DQASIQVYEETSGLGPGEPVVTTGEPLSVELGPGLISQMFDGIQRPLDRFKLATHNDF
                     LVRGVEVPSLDRDIKWHFDSTIAIGQKVSTGDILGTVKETEVVNHKIMVPYGVSGEVV
                     SIASGDFTIDEVVYEIKKLDGSFYKGTLMQKWPVRKARPVSKRLIPEEPLITGQRVID
                     AFFPVTKGGAAAVPGPFGAGKTVVQHQVAKFANVDIVIYVGCGERGNEMTDVLNEFPE
                     LIDPNTGQSIMQRTVLIANTSNMPVAAREASIYTGITMAEYFRDMGYSVAIMADSTSR
                     WAEALREMSGRLEEMPGDEGYPAYLGSRIAEYYERAGRSQVLGLPEREGTITAIGAVS
                     PPGGDISEPVTQNTLRIVKVFWGLDAPLAQRRHFPAINWLTSYSLYKDSVGTYIDGKE
                     KTDWNSKITRAMNYLQRESSLEEIVRLVGIDSLSDNERLTMEIAKQIREDYLQQNAFD
                     SVDTFTSFAKQEAMLSNILTFADQANHALELGSYFTEIMEGTVAVRDRMARSKYVSED
                     RLDEIKIISNEITHQIHLILETGGL"
     gene            3163..4548
                     /locus_tag="VVL45_002033"
     CDS             3163..4548
                     /locus_tag="VVL45_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEU4188156.1"
                     /translation="MSVIKEYRTASEVVGPLMIVEQVNNVSYNELVEIQLHNGEIRRG
                     QVLEIHEDKAMVQLFEGSSGINLEKSKIRFAGHALELAVSEDMVGRIFNGMGKPIDGG
                     PDLIPEKYLDIDGQAINPVSRDYPDEFIQTGISSIDHLNTLVRGQKLPVFSGSGLPHN
                     ELAAQIARQATVLNSDENFAVVFAAMGITFEEAEFFMEELRKTGAIDRSVLFMNLAND
                     PAIERIATPRIALTAAEYLAFEKDMHVLVIMTDMTNYCEALREVSAARREVPGRRGYP
                     GYLYTNLSTLYERAGRLVGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSHE
                     LYNQGYRPPINVLPSLSRLKDKGSGEGKTRGDHAPTMNQLFAAYAQGKKVEELAVVLG
                     ESALSDVDKLYVRFTKRFEEEYINQGFYKNRNIEDTLNLGWELLSILPRTELKRIKDD
                     LLDKYLPLVEV"
     gene            4571..5182
                     /locus_tag="VVL45_002034"
     CDS             4571..5182
                     /locus_tag="VVL45_002034"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEU4188157.1"
                     /translation="MVRLNVKPTRMELNNLKERLTTAERGHKLLKDKRDELMRRFISL
                     IRENNQLRKEVESYLIDNLKSFAVAKSLKNSQMVEELFSIPSKEIELFVEKENIMSVT
                     VPRMHMNITSQNENSEYSYLSSNSEMDDVFATMNSLIYKLLRLAEVEKTCQLMADEIE
                     KTRRRVNGLEYSIIPNLSETIHYIELKLEEAERANLVRIMKVK"
BASE COUNT         1792 a          769 c         1109 g         1736 t
ORIGIN      
        1 tattttatgg atactaatct tttttcaaaa ataaatacga cgatttcggt aaaagaaaac
       61 gattttatta cagaagaaaa atttcaaaaa attatacaat ccaaagatac ggagacattg
      121 gcatttatct tagaatcaac tccctatcat ttatcgattg acatcttaga agatcctagt
      181 cagacagaga tttcgctaat gacaaaatta gtcaatgatt atagatgggc ctatgctgaa
      241 agtccgtctg atataattgt gactttattt gctttacgat atgtttatca taatatcaaa
      301 gttttattaa aatctaaggc ggcaattaag aaagattttt ctaaattatt aattccaata
      361 gggatttttg atatagaaag tttaaaacat ttagtttctt ccttacattc agatacactt
      421 cctgatttta tggttcgtga agtagaatca atttggaatg agtatgaaac ttttaataat
      481 attcgtgtac ttgatgtcgg agctgatcta gcatatttta aacatctgaa acttttatct
      541 aatgagttag atgaggtact gtctcaggtt attgtcgaaa tgattgactt ttataatatt
      601 attactgtaa aacgtggttt atctcaaaat aagagtcatg gggatatttt acaattactt
      661 tcagatgaag gaagtatttc tgctaaagaa tttatataca ttgtagaaaa tcaagaaata
      721 tttgtgtggt tcaataaaat aaatccaagc ttagattcaa tcttttcaac ttatgaattg
      781 aagatgcagg acgcaacaat ttcatcttct gagttagaat ttttatgtga tttactattg
      841 tataaaactt tagatcaagg aaggtacaat gtagaggggc cgttagttct tgctagatat
      901 ttattgggat gtgagtttga agtaaagaat ctcagaatga tcatatcagc tcttcaaaat
      961 acaattccct ttgaatcaat aaaagaaagg atacgcccac attatggaag ctaataagta
     1021 taaaattggc ataattggta gccgtgatat tattttacca tttagcatga ttgggtttga
     1081 tatatttcct gcctaccaag aacaagaagc tataaataca ctaagaaaat tagctcaatc
     1141 tgattatggt gtcatttata tcactgaaga cattgcttca atgatattag atacaattcg
     1201 ccattatgat tcccaagttg tgcctgctat tattttatta ccgactcata aacaaggttt
     1261 aaatttagga ttaaaacgta tagaggataa tgtagagaaa gcagtaggac acaatatttt
     1321 ataataatgt acaaaattgt ctgtaatatt attctataat ttttggactt agtaaggaga
     1381 ataactttga ctcaagggaa gattataaaa gtatcgggac ctctagttat tgcatcaggt
     1441 atgcaggagg ctaatattca agatatttgc cgtgtaggta agctagggtt aatcggtgaa
     1501 attattgaaa tgagaagaga tcaggcatct atccaagtct atgaagaaac atctggtctt
     1561 ggtccgggag aacctgttgt tacaactgga gaacctctct cggttgaatt agggccagga
     1621 ttgatttctc aaatgtttga tggcatacaa cgcccattag atcgatttaa attggctact
     1681 cataatgatt ttctagttcg tggggtagaa gttccaagtt tggatagaga tattaagtgg
     1741 cattttgatt ccactatagc aattggtcaa aaagtgagta cgggtgatat tcttggaact
     1801 gtcaaggaaa ccgaggtagt taatcataaa attatggttc cttatggagt atctggagaa
     1861 gtcgtttcta ttgcatctgg cgattttaca attgatgaag ttgtatatga aataaaaaaa
     1921 ttggacggta gtttctataa aggaacgctt atgcaaaaat ggcctgtccg caaggcgcgt
     1981 cctgtttcta aacgtttaat tccagaagaa ccattaatca caggtcaacg agttattgat
     2041 gcattctttc cagtaaccaa agggggagct gcagcagttc ctggaccgtt tggagcagga
     2101 aagacagttg tacaacacca agtagctaaa tttgccaatg ttgatattgt tatttatgtc
     2161 ggttgtggag aacgtggaaa tgaaatgacg gatgtactga atgagtttcc tgagttgatt
     2221 gaccctaata ccggacaatc aattatgcaa cggacagttc tgattgctaa tacttcaaat
     2281 atgcctgttg ctgctcgtga ggcttcaatt tatacaggaa ttaccatggc tgagtatttt
     2341 cgtgatatgg gctactctgt cgccattatg gctgattcaa cttcacgttg ggcagaagcg
     2401 ctacgtgaaa tgtcaggacg tctagaagaa atgcctggtg atgagggtta tcctgcttat
     2461 ctgggaagtc gtatcgctga atattatgaa agagcaggac gttctcaggt tctagggctt
     2521 ccagaacgtg aaggaacgat tactgctatt ggagctgtat cgccacctgg tggagatatt
     2581 tcagaaccag ttactcaaaa cactttacgg attgtgaaag ttttttgggg gcttgatgct
     2641 ccgttggcac agcgacgtca ttttcctgca attaactggc ttacatctta ttcactatat
     2701 aaagacagtg tgggcactta tatagatggt aaagagaaga cagattggaa tagtaaaata
     2761 actcgtgcga tgaactactt acaacgggaa tctagtttag aggaaattgt tcgtcttgtt
     2821 ggaattgatt ctctgtctga taatgaacga ctaacgatgg aaattgctaa acaaattcga
     2881 gaagattatt tgcaacagaa cgcttttgat tcggtagata cattcacttc gtttgcaaaa
     2941 caagaagcaa tgctaagtaa tattctcact tttgctgatc aggcaaatca tgctttagag
     3001 ttgggttctt actttacaga gattatggaa ggtaccgtgg cagttcgaga ccgtatggcg
     3061 agaagtaaat atgtttcaga agatagatta gatgaaatca aaattatatc aaatgagatt
     3121 acacatcaaa ttcatttgat attagaaaca ggaggtctat aaatgagtgt tataaaagaa
     3181 tacagaactg ctagtgaagt tgttgggcct cttatgattg ttgaacaagt aaataatgtg
     3241 tcttacaatg agttagttga aattcaactt cataatggag aaattcgtcg tggacaagtt
     3301 ttagagatcc acgaagataa agcaatggtt cagctttttg aaggatctag tggaataaat
     3361 ttagaaaagt ctaaaattcg ttttgctggt catgcattag aattggctgt atctgaggat
     3421 atggttggtc gtatttttaa tgggatggga aaaccaattg atggtggacc agatttaatt
     3481 ccagagaaat atttagatat tgatggtcaa gctattaatc ctgtatctag agattatcca
     3541 gatgaattta ttcagacagg gatctcctct attgatcatt tgaatactct tgtacgtggt
     3601 caaaaattac cagtattttc aggttcgggc ttacctcata atgaattagc tgctcagata
     3661 gcaagacaag cgactgtttt aaattctgat gaaaattttg cggttgtatt tgcagcaatg
     3721 ggtattactt ttgaagaagc tgagtttttt atggaagaac tcagaaaaac aggagcgatc
     3781 gatcgttcgg ttttatttat gaacttggca aatgatcctg caattgagcg tattgcaact
     3841 ccccgcattg ctttaactgc ggcagagtat ctagcttttg aaaaagatat gcacgttcta
     3901 gttatcatga cggatatgac taactattgt gaagcgttac gtgaagtctc ggcagctcgc
     3961 cgtgaagttc cagggagacg aggctatccg ggatatttat atacaaattt atcaactcta
     4021 tacgaaaggg ctggtcgctt agttggtaaa aaaggttcgg tgacacagat tcctatttta
     4081 acaatgccag aagatgacat aacacatcca attcctgatt taactggata cattactgaa
     4141 gggcaaatta ttttgtcgca tgagttgtat aatcaaggtt atcgtccacc aatcaatgtt
     4201 ttaccttctc tctctcgatt aaaagataag ggatctggag aaggtaaaac tcgtggagat
     4261 catgctccaa ctatgaatca actgtttgca gcctatgccc aagggaaaaa ggttgaagag
     4321 ttagcagtag tattaggaga atcggcttta tctgatgtag ataaattgta tgtgaggttt
     4381 acaaagcgtt ttgaagaaga gtacataaac caaggatttt ataaaaatcg aaatatagaa
     4441 gatacgttga atcttgggtg ggaattacta tcaattcttc ctagaacaga gttaaaacgt
     4501 atcaaagatg atttgcttga taaatactta cctttggtag aagtttaatc cggaaatgga
     4561 gtgattatct atggtacgtt tgaatgtaaa accaactcgt atggaattga ataacttaaa
     4621 ggaacgtttg acaacagctg aacgtggaca taagttatta aaggataaaa gagatgaatt
     4681 gatgaggcga tttatttctt tgattcgtga gaataatcaa cttcggaaag aagtggaaag
     4741 ttatctaatt gataatctaa aatcctttgc agttgctaaa tcattaaaga attctcaaat
     4801 ggtggaggaa ttattttcaa ttccatcgaa agaaattgaa ttatttgttg agaaagaaaa
     4861 tatcatgagt gtaacagttc ctagaatgca tatgaatatt acttctcaaa atgagaacag
     4921 tgaatacagc tatttatctt ctaatagtga aatggatgat gtatttgcta caatgaatag
     4981 tttaatttat aaattactaa gactggcaga agttgaaaaa acgtgtcagt taatggctga
     5041 tgaaatagaa aaaacacgta gacgtgtaaa tggtttagaa tactcgatta ttccaaactt
     5101 gtcggaaact attcattata tagaattgaa actagaggag gcagaaagag ccaatttagt
     5161 tcgtattatg aaagtgaagt agatccttta tttagattat taattagatg aacaaatatc
     5221 agcttggata aggctttaag cctttctaag ctttttttat tgacagtatc aggatatctt
     5281 tttcaaaatt ttggtttgtt agataatgaa aatgtttcta ctaatctaga tttaggatta
     5341 gtaaatcgta aatgtaatta tatagaaagt aagcgcgtca taacaaggta tctatcattc
     5401 atggag
//
LOCUS       DAVHEX010000091         5218 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVHEX010000091 DAVHEX010000000
VERSION     DAVHEX010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5218
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.092"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            84..850
                     /locus_tag="VVL45_002035"
                     /pseudo
     CDS             84..850
                     /locus_tag="VVL45_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850213.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1682..2038
                     /locus_tag="VVL45_002036"
     CDS             1682..2038
                     /locus_tag="VVL45_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188158.1"
                     /translation="MKRDVRDIRKQFRLTEAEEKQILALMRERGETNFSDFLRKSLLS
                     SDLQKQMETWFALWQSQKLEQISRDVHEVLILAQSERQVTQEHVSILLTCVQELIQEV
                     ANTIPLSKEFREKYMR"
     gene            2043..2408
                     /locus_tag="VVL45_002037"
     CDS             2043..2408
                     /locus_tag="VVL45_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEU4188159.1"
                     /translation="MEHRYRTNLKKVFLSDSELNQLNINIDQSGCKSFSEYARRTLLD
                     PGMNFITIDTNGYQDLVFELKRIGNNINQIARSVNQSQLISGEELQELKKGIGELIKE
                     VDKEFNLQAQKLKEFHGHH"
     gene            2395..4218
                     /locus_tag="VVL45_002038"
     CDS             2395..4218
                     /locus_tag="VVL45_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002917262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEU4188160.1"
                     /translation="MVITKHFAIHGKSYRRKLIKYILNPEKTNNLALVSDYGMKNFLD
                     FPSYEEMVQMYHENFISNDTLYDFRHDRMEENQRKIHAHHIIQSFSPEDHLTPEQINR
                     IGYETVKELTGGKFRFIVATHVDKDHLHNHIIINSVDSNSDKKLKWDYKVERNLRMIS
                     DRFSKIAGAKIIENRYSHQRYEVYRKTNHKYELKQRLYFLMEHSRDFEDFKKNAPLLH
                     VEMDFRHKHATFFITDSTMKQVVRGKQLNRKQPYTEEFFKNYFAKREIESLMEFLLLK
                     VENMDDLLQKAKLFGLTINPKQKHVSFQFAGVEVKETELDQKNLYDVEFFQDYFKNRK
                     DWQAPETEDFVQLYQEEKLSKEKELPSDEKFWESYQEFKSNRDAVHEFEVELSLNQIE
                     KVVDDGIYVKVKFGIRQEGLIFVPNMQLDMEEDKVKVFIRETSSYYVYHKDAAEKNCY
                     MKGRTLIRQFSYENQTIPLRRKATVDMIKEKIAEVDALIELEVENQSYVTIKDELVHE
                     LAASELRINELQERMSTLNQVAEYLLASVESKQEMKLNLSKLNITENISANIVEKKLK
                     SLGNQLELERGRYEKMVVRLDKFINRLNTGLSKGDGIDFQK"
     gene            4332..5198
                     /locus_tag="VVL45_002039"
     CDS             4332..5198
                     /locus_tag="VVL45_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU4188161.1"
                     /translation="MGTLLATRLKNRRKELKMSQRELAEGICKQGQISRLESGEFTPG
                     ADFLHALAKKLKVSMDYFFDEQVVEEVNELSEFKKLAQTFITNRNYESLKYIYELENV
                     KAHRLSLADKFYMEWIKSLIDFYFYGQKEEAITRLENMLPQLNVSDLNYLQVSNTLFN
                     FYYDIEDLERFNEIRDKLEHQVNQLNLNTIEELELSIKFNYNVCRYLWLQKNIEEAIT
                     KITATIKQCKEYRTTYLLADLYLLMGSVSENFSSKSSVKEYFETAHFLYKLEENMSMA
                     LKVEHYFADITE"
BASE COUNT         1860 a          783 c         1062 g         1513 t
ORIGIN      
        1 aaaatggaat agaaatagtt gtagtaactg atcataatac taccaaaggt attaaaaagt
       61 tacaaatggc agtctcaatc ataatgaaaa attatccgat ttatgatata catcctcata
      121 ttttacatgg agtagaaatt agtgcagcag ataaattgca tattgtatgt atatatgatt
      181 atgaacaaga atcatgggtt aatcaatggt taagtgaaaa tattataagt gagaaagatg
      241 gaagttatca acattcactg actataatga aggatttcaa taatcaaaaa atagttaact
      301 atattgctca tttcaatagt tatgacattt tgaaaaaagg ttctcactta tcaggtgcat
      361 ataaacgaaa aattttttct aaagaaaata cacgattttg gagtttaata ttaactcgaa
      421 agaatcttcg caacaacttg atattctcta taaagaagtt ggtgtattaa gtttgggaca
      481 aaaagttgta gccatgcttg attttttatt agcatatagt gattattcta aagacttcag
      541 accattgatt attgatcagc ctgaagacaa tctagacaat cgttatattt acaggcattt
      601 agttcagcag tttagagatg tgaaagctca acgtcaaatt attttagcaa cacataatgc
      661 tacaattgta acaaattcta tgacagatca agttgttatt atggagtcag atggagttaa
      721 cggatggatt gaatcacagg gatatgttag tgaaaaatat ataaaaaatc atatcatcaa
      781 tcaattagag ggaggaaaag attccttcaa gcataaaatg tctatatatg agacggcttt
      841 atcagagtag agtcagaaaa agtaggttag aaatttagcc tacttttttc tttgtccgac
      901 aggcatagtg tacatctgag gtccaagtcc tctgtggata tttgctgcag atgaaaccaa
      961 tagcgactcc taagcctgaa tatcgtgagg taggggggat aggaaggaat tagcgaaatc
     1021 aaggttctac aaacagaatc gtgacttgaa gccatatata gcggatgagg aactctaaaa
     1081 tccaaatagg tgtcgtaacc tatatacgta aattacgaga gtaaactagg aaagatgtac
     1141 ggcttattcc gtgagcgttt aggacgtagt acaacgaatc atgggagtca gctgaacaca
     1201 tagtattgaa gaaatttctg taatggaaat ggagcgaaga agtgaacaat taaatgaata
     1261 cctctctaat taaatttgtc aattctaatt cctggtatga aaagacagtg acctgaaaat
     1321 gtaaacgatg ggagctgatc ataaatatag gacggtacat gcagtggtgt tagagattag
     1381 tccttacttg atttgtgata acttccccaa atttcttctg ctatactttt ctcaactttt
     1441 aaaaatccaa ctaagaattt tacctggggg tttgggggcg gagccactaa gttatcttat
     1501 cgttagctgt caaaactggt aggttttgat aggctggcga tatgattttt gggatattgt
     1561 ggacacaata tctgagctcg caaagcctta caagaatgaa aatcagttgt tggaaaagtg
     1621 tactgacatt gtatggtagc tcacattgtc agtacaagta ttttggaaag gaagtagcag
     1681 tatgaaacga gatgtgcgtg atattcggaa acaatttcgt ttaacagaag cagaagaaaa
     1741 gcaaattcta gctttgatga gagagcgggg agagactaat ttctctgatt ttcttcgtaa
     1801 aagtttactt tcctctgatt tacaaaaaca gatggagaca tggtttgccc tctggcaatc
     1861 ccaaaaacta gaacaaatca gtcgtgacgt tcatgaagtt ttaatcttgg cacagtcaga
     1921 acgtcaagtc acccaagagc atgtatctat tctcttaacg tgcgtgcagg aattgattca
     1981 agaggttgca aacaccatac ccctcagtaa agaatttcgt gagaagtaca tgaggtaagc
     2041 acatggaaca tcgttaccga accaatctca agaaagtgtt tttgtctgat agtgagttga
     2101 accaactaaa tataaatatc gatcaaagtg gttgtaaatc cttttctgaa tatgcgagac
     2161 gaactctact cgatcctggt atgaatttta tcacgattga cacaaacggt taccaagatt
     2221 tagtgtttga gttaaagagg attggcaata atatcaacca gattgctcga agtgttaatc
     2281 aatctcagtt aatttctggt gaagaattgc aggagttgaa aaaaggaatt ggtgaattga
     2341 taaaagaagt tgataaggaa tttaatctgc aagcgcagaa gctaaaggag ttccatggtc
     2401 atcactaaac actttgccat tcacggaaag agttaccgca gaaagcttat caagtacatt
     2461 ctcaatcctg agaaaaccaa taatcttgcc ttggtgtcgg actatggcat gaagaatttt
     2521 ctggactttc ctagctatga ggaaatggtg cagatgtatc atgaaaattt catcagcaac
     2581 gatacgcttt acgattttcg ccacgacagg atggaagaaa atcaacgaaa aatacacgct
     2641 caccacatca ttcagtcttt ctcgccagag gatcatctca ctcctgaaca aatcaatcgg
     2701 ataggttatg agactgtgaa ggaattaact ggtggcaaat ttcgttttat cgttgcgacc
     2761 catgttgata aagaccacct gcacaatcac atcattatca attcagtaga tagcaattct
     2821 gacaaaaagc tcaagtggga ctacaaggtg gagcgaaatc ttcgcatgat ttctgaccgt
     2881 ttttctaaaa tcgcaggtgc taaaatcatt gagaaccgct attctcacca gcggtatgaa
     2941 gtctatcgta agactaatca caagtatgaa ctcaagcagc gactctattt tttgatggaa
     3001 cattctaggg actttgagga tttcaaaaag aatgctccgc tactacatgt ggagatggat
     3061 ttccgtcaca agcatgccac cttttttatt acggactcaa ctatgaaaca ggtggtgcgt
     3121 ggcaagcaac tcaatcgcaa gcagccttac acagaagaat tttttaagaa ctactttgcc
     3181 aaaagagaaa tagaaagtct catggaattt ttattgctga aagttgagaa tatggatgat
     3241 ttacttcaga aagcaaaact ttttggacta actatcaatc ctaaacaaaa gcatgtttct
     3301 tttcaatttg caggagtgga ggtaaaggag acagagctag accagaaaaa tctttatgat
     3361 gtagagtttt tccaagatta ttttaaaaat agaaaagatt ggcaagctcc agaaactgag
     3421 gatttcgttc aactttatca agaagaaaag ttatccaaag aaaaagaact tccaagcgat
     3481 gagaagttct gggagtccta tcaagagttc aagagtaaca gagatgccgt tcatgaattt
     3541 gaggtggagt tgtcactcaa tcaaattgaa aaagtagtgg atgatggaat ttacgtcaag
     3601 gtcaagtttg gtattcgtca ggagggactt atctttgtgc cgaacatgca gcttgatatg
     3661 gaagaggata aggtgaaggt tttcatcagg gaaaccagct cctactatgt ctaccacaaa
     3721 gacgctgccg agaaaaattg ttatatgaaa ggtcgaacct taattagaca gttcagctat
     3781 gaaaatcaaa ccattccatt acgcagaaaa gcgacagtcg atatgattaa agagaagatt
     3841 gcggaagtgg atgctttgat tgaactggaa gtagaaaatc aatcttatgt cacgattaaa
     3901 gatgagttag tgcatgaact agcagcgtct gaattgagaa tcaatgagtt gcaagaacga
     3961 atgtcaacct tgaatcaagt agcagaatat ctactggctt cagttgaaag taagcaagaa
     4021 atgaaattaa atctttcaaa actgaatata actgagaata tcagtgctaa tattgttgag
     4081 aaaaaattga agagcctggg gaatcaactg gaattggaaa ggggcaggta tgaaaagatg
     4141 gtagtcaggc tagataaatt tataaatcgc ttaaatacag gactttcaaa aggagatggg
     4201 atagattttc aaaaatagga atctagccaa taattatata caatgtgtat cttttgtaaa
     4261 atatatttga aatttagatt taatcatgtt acaattaaca ttgaaatcaa atagaaaagg
     4321 aagatgcaaa cgtgggtaca ctattagcaa caagattaaa aaatagacga aaagaattaa
     4381 aaatgtctca gcgagaatta gctgagggta tctgtaaaca ggggcaaatt agtcgattag
     4441 agagtggaga gttcacccct ggagcggatt ttttacatgc ccttgctaag aaattaaaag
     4501 ttagtatgga ctactttttt gatgagcagg ttgtagagga agtcaatgag ttatcagagt
     4561 ttaagaagtt agcacagaca tttatcacaa atcgtaatta tgaatcttta aaatatatat
     4621 atgaattgga aaatgtaaaa gcccatcgct tatctctagc agataaattt tacatggaat
     4681 ggataaagtc tcttatagat ttttattttt atggacagaa agaagaggca attacacgat
     4741 tagagaatat gctacctcag ttaaatgtat ctgatttgaa ctaccttcaa gtttcaaaca
     4801 ctctattcaa tttttattat gatattgaag atttagaacg ttttaatgag attagagaca
     4861 agctagagca tcaagtgaat cagcttaatt taaacacaat tgaagagtta gaactatcaa
     4921 ttaagttcaa ttataacgtt tgtcgatacc tatggttaca gaaaaatatt gaagaagcta
     4981 ttacgaaaat cacagctacg ataaagcaat gtaaggagta caggacaact tatctattag
     5041 ctgatttgta tttattgatg ggaagtgtaa gtgaaaattt ttcttctaaa agttcagtaa
     5101 aagaatattt tgagactgct cattttctct acaaacttga ggaaaacatg tcaatggctt
     5161 tgaaagtcga acattatttt gcagatataa cagaataagg aatacggtgt tctagtga
//
LOCUS       DAVHEX010000092         5068 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVHEX010000092 DAVHEX010000000
VERSION     DAVHEX010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5068
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.093"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(409..1137)
                     /locus_tag="VVL45_002040"
     CDS             complement(409..1137)
                     /locus_tag="VVL45_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU4188162.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
     gene            1295..2704
                     /locus_tag="VVL45_002041"
     CDS             1295..2704
                     /locus_tag="VVL45_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEU4188163.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLGLDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKAIYDIAMIVKKEYGNIPWFISENGMGVENEARFIGENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKRS
                     GRWYRKVSDNNGFEVEIEE"
     gene            2722..4017
                     /locus_tag="VVL45_002042"
     CDS             2722..4017
                     /locus_tag="VVL45_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU4188164.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            4022..4330
                     /locus_tag="VVL45_002043"
     CDS             4022..4330
                     /locus_tag="VVL45_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU4188165.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            4327..4635
                     /locus_tag="VVL45_002044"
     CDS             4327..4635
                     /locus_tag="VVL45_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU4188166.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
BASE COUNT         1721 a          844 c          924 g         1579 t
ORIGIN      
        1 tgatttagaa tttatttaat atattaaaag aagatatgaa caaataaatt gggagattga
       61 aattcatttc tagcaatttg ttagaagtca cagtctacta ttctaaccac agtatactat
      121 atgtgttaca agaaaagact caaaaacttc tcgtcagtgt aagaatgttt gaaacactat
      181 ttaaaactaa ataactataa gaagtagatc aggtgaacaa tttcagtacc tactctataa
      241 gctatacgaa gatatttttc aacatgatgg atacaacgat cactctttta aagccatctt
      301 atttagaaaa gagaaaccat ttaagcatag atgggacctt cttgccctgt tttaaatttt
      361 tcttaaacta gagtctgtaa aagattggct tcattttcac attttctcct aggaggaatg
      421 tcgtaatgca aaagaattaa attcaaataa atcatagcga tgacgactaa tcgagtactc
      481 aaacggagtt ccgttatcca aataagccac ttgttctact tcaaataccg gttccgttgg
      541 aaggagctgc aagtaatgtt gctcattttc tgaactagaa gaagctcgta agatttttgt
      601 agcactggca atatgcaatc ctaactttcc ttcaatgtaa ttgtaaatcg atttttgtaa
      661 aatatcttca gtaatacctg gaataacatc ggtactcata taagtttgtt ccagaacata
      721 aggtttaccg tcaataatac gcaggcggta aatattataa actggactct gcaaagcgac
      781 ctgtagtttt tctgctaaaa attcatttgc aaattctaat ttgaagtgaa taatcctcga
      841 gtcaattgta gaatgagcat cttcagatat ttttgtcagt ccccggatat ctctttctgg
      901 aacgattaat tttctttttg agctgccacg agagagaaca aaggttccct gcccacgttt
      961 tctaaaaatt aagccctcag aaactaacaa gtctaaagct tttttgatcg tcattcgact
     1021 acaggcaaac tgtttagcta atgtcatttc atcaggcaat tgttcgttca tggtatattt
     1081 attacaaaga atcttttcac gaatctcatt gtaaataaat aaatattttg tactcattta
     1141 atctggttct ctctataata ttctaatagt agtataacaa agttcaagaa atgaggcaaa
     1201 cacaagatat gtatcaacaa tttttaaaaa tgtctagaca aattttaaaa ttatgctata
     1261 ctttatgtgt agaataatag aaaggaggtt gagcatgaaa aaaaagttag tatttcctaa
     1321 tctgttttgg tggggagctg cttctagcgg acctcagaca gaaggtcaat atggaaaagt
     1381 acatgaaaat gtgatggact actggttcaa aacgcatcca gaagattttt tcgataatgt
     1441 cggacctctt gtagccagta acttttttca tacttacacc gaagatttcc acttgatgaa
     1501 ggaaattgga gttaattctt tccgcacttc catccaatgg agtcgactca tcaagaattt
     1561 agagacaggt gagcctgatc caaaaggtat tgctttctac aatgccatca ttgaagaagc
     1621 taaaaagaac cagatggatc ttgtgatgaa tttacatcat tttgatttac cagtggaact
     1681 tcttcaaaaa tacggtggtt gggaaagcaa acatgtagtg gagttattcg tgaagtttgc
     1741 caagactgct ttcacatgct ttggagataa ggttcattac tggacaactt tcaatgagcc
     1801 aatggtcatt ccagaagcag gatacttata tgctttccat tatccaaatc taaaaggaaa
     1861 gggaaaagag gccgtacaag tcatctataa tctaaacctt gctagtgcaa aagtgattca
     1921 actatatcgc tcattaggac ttgatggaaa gattgggatt attttaaact tgacacctgc
     1981 ttatccaaga agtaattctc cagaagactt agaagcaagt cgatttacag atgacttctt
     2041 taacaaagtc ttcttgaatc cagctgttaa aggaactttc ccagaaagat tggtaaaaca
     2101 gctagagaga gatggcgtgt tatggagtca taccgaaaaa gagcttcaac tgatgaaatc
     2161 aaatacggtt gattttcttg gagtaaacta ctaccatcca aaacgtgttc aagcacaagc
     2221 aaatcctgag gaatatcaga cgccctggat gccagaccaa tacttcaaag agtatgaatg
     2281 gccggagcgt cgcatgaatc catatcgtgg ttgggaaatt tttccgaaag ccatttatga
     2341 tattgctatg attgtgaaga aagaatatgg taatattcca tggtttatca gtgaaaacgg
     2401 aatgggtgtt gaaaacgaag cacggtttat cggtgaaaat ggagttatcg atgacgtgta
     2461 tcgtattgaa ttttatgaag aacatttaag atggctacat aaagccattg aagagggaag
     2521 tcactgtttt ggataccacg cttggaccgc atttgattgc tggtcttgga ataatgcata
     2581 taagaatcgt tacggattta tctccgttga tttagaaacg caaaagagaa ccatcaagag
     2641 atcaggaaga tggtatcgca aagtaagtga caataacggt tttgaagtag aaattgagga
     2701 gtaaagagag gagatattca gatgaaaaac tcaaaattta tagatcaatt tgccaccttt
     2761 gctggtaaac tagggaacca aattcattta aaaaccctaa gagatgcatt cgtaacagta
     2821 atgccattat atattttggc aggtttaatc gttcttttga acaacacggt atttaagtgg
     2881 attttccaag gggatacatt aacaagattc caatattggg gaataacaat tgcaaacggt
     2941 actttaagta tttcaggtat gattattgct gtaatggttg gttatttctt agctaaaaac
     3001 agagatttcg aaaacccgtt agcagcatca atgctatcat tagtttcttt aattgtgatg
     3061 atgccaaata cagtttctgt agttcctgac ggagcaaaag atgcggtaaa catttcaggt
     3121 gttctttcat tcaacaacac aggtacaggc gcaatgttcg ccggggttat cgtagcgatt
     3181 attgcaacag aattattcat tgaattatca aacgttaaag ctttacaaat gaaccttggt
     3241 gaaaatattc caccagctgt tagtagatca tttagcgtat tacttccagt catgaccgtc
     3301 atctccttat ttggggttgt ttcagcatta ttattcaata taactggaat gaacttaatc
     3361 tcaatcatta caatctttat tcaagaacca attcgtcata ttggtacaag cttaatcggg
     3421 gtcattatta tttactcttt aggaaatatg ttatggctat ttggtattca ccaagcagtt
     3481 atttacagtg ccatcctaga accattacta ttaattaaca ttactgaaaa catcactgca
     3541 gcaaataatg gacaagccat tccacacatc atcaacctat cacaaataca aacattcgct
     3601 ttaatgggtg gtagtggatc tacattatgt ttattaatag caacattctt agtgagtcgc
     3661 aatgctgtct ctaaaaacgt ggctaaatta tcttttggac ctggtatctt caatatcaat
     3721 gaaccagtat tattcggtta cccaatcgtt tataacattt cattagctat tccatttatc
     3781 acagttccag tccttggtat tttaatcagc tacttagcaa cagttacagg attcatgagt
     3841 cctgcattta tacaagttcc ttggactaca ccagtattct taaatgcatg gttagcaaca
     3901 gcaggggacg tgagagcagt tctagttcaa ttcatcatct ttgcacttgg agttcttcta
     3961 tacattccat ttatcaaagt taatgacaaa gttgttgaac aagaaatgga aggttaagat
     4021 tatgaaacaa attttattag tatgtaatgc gggaatgtcg acaagtatgc ttgttaaaaa
     4081 aatgcaacaa agtgcgactg aacgtgggat tgaaatttct atccaagcaa aatcaatgac
     4141 agaagctaaa aagaatatcc atgaagcaga tgtcattcta attgggccac aaattcgtta
     4201 tgagttactt gctgtgaaag aaattgcagg aaatatccct gtagatacca ttgatatgcg
     4261 ggattacgga atgatgaatg gagcaaaagt tctggaacaa gcacttgcat ggataggaga
     4321 aataagatga cagaagaaat ggaatatatc tgttttcagc tcattgccaa tagtggagca
     4381 gccaaatcat cctttattga agccatccaa ctagctaagg caggaaattt aaaagaagcg
     4441 aaaattaaag ttgaagaagc ggaagactcc ttagtagaag cgcacaagat tcactctaat
     4501 ctcatacaaa aagaagctac tggagaaaaa ataggattct ccttgctatt tatgcatgca
     4561 gaagaccaaa tggcttcaac agagattatt cagttgctat ctaaagaatt catcgatctc
     4621 tatcaaaata aataggaaca ctgtcattcc catctgtctt tattaatgac ggctacattg
     4681 taggaacctc caaaagttgg acaaaatgat tgaaaggatt ggatcctgca ttacacaaga
     4741 tccaatcctt ttcttttact ctagttttta taatgtgttg aatttagaat agtccactgc
     4801 agtttctaaa aaattgctgg aaatagatgt taagctccaa tttatttgtt tatatcttat
     4861 ttcagtttac tatactagct tattattcta gtaaaaaatt gaatgagcca ctatttattt
     4921 aatatgtatg atgagggaat gactcaaaaa cttcttgtcg atataagaat atctgagata
     4981 gtattttaaa acgaaaggat tataaaagct catttccttc gcacttattt tatagagatc
     5041 tatcattata ctctttagaa atcaaaaa
//
LOCUS       DAVHEX010000093         5041 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVHEX010000093 DAVHEX010000000
VERSION     DAVHEX010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5041
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.094"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            174..1719
                     /locus_tag="VVL45_002045"
     rRNA            174..1719
                     /locus_tag="VVL45_002045"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1771..1843
                     /locus_tag="VVL45_002046"
     tRNA            1771..1843
                     /locus_tag="VVL45_002046"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1804..1806,aa:Ala,seq:tgc)
     gene            1968..4869
                     /locus_tag="VVL45_002047"
     rRNA            1968..4869
                     /locus_tag="VVL45_002047"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            4946..>5041
                     /gene="rrf"
                     /locus_tag="VVL45_002048"
     rRNA            4946..>5041
                     /gene="rrf"
                     /locus_tag="VVL45_002048"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1420 a         1026 c         1480 g         1115 t
ORIGIN      
        1 agcagttgtg atatactaat atagttgtcg cttgagagaa gcaagtgaca aagacctttg
       61 aaaactgaac aagacgaacc aatgtgcagg gcgctacaac gtaagttgta gtactgaaca
      121 atgaaaaaaa caataaatct gtcagtgaca gaaatgagta agaactcaaa ctttttaatg
      181 agagtttgat cctggctcag gacgaacgct ggcggcgtgc ctaatacatg caagtagaac
      241 gctgaaggag gagcttgctt ctctggatga gttgcgaacg ggtgagtaac gcgtaggtaa
      301 cctgcctggt agcgggggat aactattgga aacgatagct aataccgcat aagagtagat
      361 gttgcatgac atttgcttaa aaggtgcact tgcatcacta ccagatggac ctgcgttgta
      421 ttagctagtt ggtggggtaa cggctcacca aggcgacgat acatagccga cctgagaggg
      481 tgatcggcca cactgggact gagacacggc ccagactcct acgggaggca gcagtaggga
      541 atcttcggca atggacggaa gtctgaccga gcaacgccgc gtgagtgaag aaggttttcg
      601 gatcgtaaag ctctgttgta agagaagaac gagtgtgaga gtggaaagtt cacactgtga
      661 cggtatctta ccagaaaggg acggctaact acgtgccagc agccgcggta atacgtaggt
      721 cccgagcgtt gtccggattt attgggcgta aagcgagcgc aggcggttag ataagtctga
      781 agttaaaggc tgtggcttaa ccatagtagg ctttggaaac tgtttaactt gagtgcaaga
      841 ggggagagtg gaattccatg tgtagcggtg aaatgcgtag atatatggag gaacaccggt
      901 ggcgaaagcg gctctctggc ttgtaactga cgctgaggct cgaaagcgtg gggagcaaac
      961 aggattagat accctggtag tccacgctgt aaacgatgag tgctaggtgt tagacccttt
     1021 ccggggttta gtgccgtagc taacgcatta agcactccgc ctggggagta cgaccgcaag
     1081 gttgaaactc aaaggaattg acgggggccc gcacaagcgg tggagcatgt ggtttaattc
     1141 gaagcaacgc gaagaacctt accaggtctt gacatccctc tgaccgctct agagatagag
     1201 ctttccttcg ggacagaggt gacaggtggt gcatggttgt cgtcagctcg tgtcgtgaga
     1261 tgttgggtta agtcccgcaa cgagcgcaac ccctattgtt agttgccatc atttagttgg
     1321 gcactctagc gagactgccg gtaataaacc ggaggaaggt ggggatgacg tcaaatcatc
     1381 atgcccctta tgacctgggc tacacacgtg ctacaatggc tggtacaacg agtcgcaagc
     1441 cggtgacggc aagctaatct cttaaagcca gtctcagttc ggattgtagg ctgcaactcg
     1501 cctacatgaa gtcggaatcg ctagtaatcg cggatcagca cgccgcggtg aatacgttcc
     1561 cgggccttgt acacaccgcc cgtcacacca cgagagtttg taacacccga agtcggtgag
     1621 gtaaccgtaa ggagccagcc gcctaaggtg ggatagatga ttggggtgaa gtcgtaacaa
     1681 ggtagccgta tcggaaggtg cggctggatc acctcctttc taaggataag gaactgcgca
     1741 ttggtcttgt ttagtcttga gaggtcttgt ggggccttag ctcagctggg agagcgcctg
     1801 ctttgcacgc aggaggtcag cggttcgatc ccgctaggct ccattggtga gagatcacca
     1861 agtaatgcac attgaaaatt gaatatctat atcaaatagt aacaagaaaa taaaccgaaa
     1921 acgctgtagt attaataaga gtttatgact gaaaggtcag aaaataaggt taagttaata
     1981 agggcgcacg gtggatgcct tggcactagg agccgacgaa ggacgtgaca aacgacgata
     2041 tgccttgggt agctgtaagt aagcgatgat ccagggattt ccgaatgggg gaacccaaca
     2101 ggtaatacct gttacccaca tctgttaagg atgtgaggag gaagacgcag tgaactgaaa
     2161 catctaagta gctgcaggaa gagaaagcaa aagcgattgc cttagtagcg gcgagcgaaa
     2221 cggcagaagg gcaaaccgaa gagtttactc ttcggggttg taggactgca atgtggactc
     2281 aaagattata gaagaatgat ttgggaagat cagccaaaga gagtaatagc ctcgtattta
     2341 aaatagtctt tgtacttagc agtatcctga gtacggcggg acacgtgaaa tcccgtcgga
     2401 atctgggagg accatctccc aaccctaaat actccctagt gaccgatagt gaaccagtac
     2461 cgtgagggaa aggtgaaaag caccccggga ggggagtgaa atagaacctg aaaccgtgtg
     2521 cctacaacaa gttcgagccc gttaatgggt gagagcgtgc cttttgtaga atgaaccggc
     2581 gagttacgtt atgatgcgag gttaagttga agagacggag ccgtagggaa accgagtctg
     2641 aatagggcgc cttagtatca tgacgtagac ccgaaaccat gtgacctacc catgagcagg
     2701 ttgaaggtgc ggtaagacgc actggaggac cgaaccaggg cacgttgaaa agtgcttgga
     2761 tgacttgtgg gtagcggaga aattccaaac gaacttggag atagctggtt ctctccgaaa
     2821 tagctttagg gctagcgtcg acattagaga ttcttggagg tagagcactg tttgggtgag
     2881 gggtccatcc cggattacca atctcagata aactccgaat gccaatgaat tatggtcggc
     2941 agtcagactg cgagtgctaa gatccgtagt cgaaagggaa acagcccaga ccaccagcta
     3001 aggtcccaaa ataattgtta agtggaaaag gatgtggggt tgcacagaca actaggatgt
     3061 tagcttagaa gcagctattc attcaaagag tgcgtaatag ctcactagtc gagtgaccct
     3121 gcgccgaaaa tgtaccgggg ctaaaacaat ttaccgaagc tgtggatacc tttataggta
     3181 tggtaggaga gcgttctatg tgtgatgaag gtataccgtg aggagtgctg gaacgcatag
     3241 aagtgagaat gccggtatga gtagcgaaag acaggtgaga atcctgtcca ccgtaagact
     3301 aaggtttcca ggggaaggct cgtccgccct gggttagtcg ggacctaagg agagaccgaa
     3361 aggtgtatcc gatggacaac aggttgatat tcctgtacta gagtatgtag tgatggaggg
     3421 acgcagtagg ctaactaaag cagacgattg gaagagtctg tctaagcagt gaggtgtgaa
     3481 ttgagtcaaa tgcttaattc tataacattg agctgtgatg gggagcgaag tttagtagcg
     3541 aagttagtga cgtcacactg ccaagaaaag cttctagcgt ttaaacatac tctacccgta
     3601 ccgcaaaccg acacaggtag tcgaggcgag tagcctcagg tgagcgagag aactctcgtt
     3661 aaggaactcg gcaaaatgac cccgtaactt cgggagaagg ggtgctgact taaagtcagc
     3721 cgcagtgaat aggcccaagc aactgtttat caaaaacaca gctctctgct aaatcgtaag
     3781 atgatgtata gggggtgacg cctgcccggt gctggaaggt taagaggagt gcttagcgta
     3841 agcgaaggta tgaattgaag ccccagtaaa cggcggccgt aactataacg gtcctaaggt
     3901 agcgaaattc cttgtcgggt aagttccgac ccgcacgaaa ggcgtaatga tttgggcact
     3961 gtctcaacga gagactcggt gaaattttag tacctgtgaa gatgcaggtt acccgcgaca
     4021 ggacggaaag accccatgga gctttactgc agtttgatat tgagtgtctg taccacatgt
     4081 acaggatagg taggagtcta agagatcggg acgccagttt cgaaggagac gctgttggga
     4141 tactaccctt gtgttatggc cactctaacc cagataggtg atccctatcg gagacagtgt
     4201 ctgacgggca gtttgactgg ggcggtcgcc tcctaaaagg taacggaggc gcccaaaggt
     4261 tccctcagaa tggttggaaa tcattcgcag agtgtaaagg tataagggag cttgactgcg
     4321 agagctacaa ctcgagcagg gacgaaagtc gggcttagtg atccggtggt tccgtatgga
     4381 agggccatcg ctcaacggat aaaagctacc ctggggataa caggcttatc tcccccaaga
     4441 gttcacatcg acggggaggt ttggcacctc gatgtcggct cgtcgcatcc tggggctgta
     4501 gtcggtccca agggttgggc tgttcgccca ttaaagcggc acgcgagctg ggttcagaac
     4561 gtcgtgagac agttcggtcc ctatccgtcg cgggcgtagg aaatttgaga ggatctgctc
     4621 ctagtacgag aggaccagag tggacttacc gctggtgtac cagttgtctt gccaaaggca
     4681 tcgctgggta gctatgtagg gaagggataa acgctgaaag catctaagtg tgaaacccac
     4741 ctcaagatga gatttcccat gattatatat cagtaagagc cctgagagat gatcaggtag
     4801 ataggttaga agtggaagtg tggcgacaca tgtagcggac taatactaat agctcgagga
     4861 cttatccaaa gtaactgaga atatgaaagc gaacggtttt cttaaattga atagatattc
     4921 aattttgagt aggtattact cagagttaag tgacgatagc ctaggagata cacctgtacc
     4981 catgccgaac acagaagtta agccctagaa cgccggaagt agttgggggt tgccccctgt
     5041 g
//
LOCUS       DAVHEX010000094         4759 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVHEX010000094 DAVHEX010000000
VERSION     DAVHEX010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.095"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(298..666)
                     /locus_tag="VVL45_002049"
     CDS             complement(298..666)
                     /locus_tag="VVL45_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000385025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU4188167.1"
                     /translation="MDYKVRFTEQARQDLDDIFIYYSTEFSENIAKKVITRLQQITNL
                     LIFSPEGGISFDHKIGYSLYPENMLRMIVSKQYLLFYLIHEKEVHTLRIINSRTDYLN
                     QLDHLFRTCRKLFSSQIISL"
     gene            complement(656..916)
                     /locus_tag="VVL45_002050"
     CDS             complement(656..916)
                     /locus_tag="VVL45_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP desaturase"
                     /protein_id="HEU4188168.1"
                     /translation="MSTLTRDRVYNFCVNTQLFEEAKTILESKNISLSDALNLFVEQI
                     VLEKGLTIKTADQPKAELDKGYQDIIEGRLYDADEVFSKYGL"
     gene            1241..2233
                     /locus_tag="VVL45_002051"
     CDS             1241..2233
                     /locus_tag="VVL45_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4188169.1"
                     /translation="MAMIEVEHLQKNFVKTVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCWINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPNSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            2235..3053
                     /locus_tag="VVL45_002052"
     CDS             2235..3053
                     /locus_tag="VVL45_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEU4188170.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVLYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            3055..3840
                     /locus_tag="VVL45_002053"
     CDS             3055..3840
                     /locus_tag="VVL45_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4188171.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            3879..4157
                     /locus_tag="VVL45_002054"
     CDS             3879..4157
                     /locus_tag="VVL45_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEU4188172.1"
                     /translation="MFVIEEVKDENQKKTVVAEVLKDLPEWFGIPESTQAYIEGTTTL
                     QVWTAYQESDLTGFVSLSYSSEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            4142..4339
                     /locus_tag="VVL45_002055"
     CDS             4142..4339
                     /locus_tag="VVL45_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU4188173.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFSQLWNPQNPCQILIKKLE"
BASE COUNT         1308 a          807 c         1033 g         1611 t
ORIGIN      
        1 agaaattaat tttactttcc caatcgattt gttctcatct tatttcaatc cgctatatat
       61 tatggtatcg aatcttcatc agaatgataa aattaatcaa ttaatatctg attacaaaca
      121 gaatatgaaa gctttttata tcactattga aaaatttata cgagatgatg aaagccttaa
      181 gtgttatttt acaaaggtta tttcaagtcg ttccaaggta acaagtctag atcagattga
      241 agctgataaa acgatacaaa gaaaatattc aagtgagcta aaaaaattta ttggatttta
      301 taatgagatt atttgtgagg aaaatagttt cctacatgta cgaaagaggt ggtcgagttg
      361 gtttaggtag tcggtgcgtg agttgataat tctcagggta tggacttctt tttcatgaat
      421 gaggtaaaag agcaggtatt gtttagagac aatcattctg agcatatttt ctggatagag
      481 ggagtatccg attttatgat caaagctaat accgccctct ggtgagaaga tgagtaggtt
      541 ggtaatttgt tgtagacgag tgatgacttt cttggctatg ttttcactga actctgtgga
      601 gtagtagatg aagatatcgt ctaagtcttg tctggcttgt tctgtgaagc gtactttata
      661 gtccatactt tgaaaagacc tcgtctgcgt cgtagagacg cccttcgatg atatcttggt
      721 agcctttatc tagttctgct ttgggttggt cggctgtttt aattgttaga cctttttcaa
      781 ggacgatttg ttctacaaag aggttgaggg catctgaaag gctgatgttt ttgctttcta
      841 ggatggtttt tgcttcttca aatagttgag tattgacgca gaagttataa actcggtctc
      901 gtgtaagtgt tgacatggca tttctccttg taattatatt gtattacata tatattacaa
      961 aaaagataga gaaagtcaag ggctgtttta gtagggaagg gtggtaggtt ggtaatttaa
     1021 actattaaac agaatttttg attaaaagta ttattttatg agagaaatcc taatttcaca
     1081 atccataggc aaacgcttgc atttcgtttt ttattggact ataataggtt gatataaagc
     1141 cttttgtagt aataaaatgt agaaggtgta gaaagtaagg atttagaata tttgtagtta
     1201 aaaacacaat gttgctattc cttacgatag ggagatagat atggcaatga tagaagtgga
     1261 acatcttcag aaaaattttg tgaagactgt taaggaaccg ggcttgaagg gggctttgcg
     1321 ctcctttatt catcctgaaa agcagacctt tgaagcggtc aaggatttga cctttgaagt
     1381 tccaaaaggg cagattttag gatttatcgg ggcaaatggt gctgggaagt cgacaaccat
     1441 taaaatgctg acaggaattt tgaaaccaac atctggtttt tgttggatta acggcaagat
     1501 tccccaggac aatcggcaag attatgtcaa agatattggc gtagtctttg gacaacgcac
     1561 ccagctatgg tgggatttgg ctctgcaaga gacctacact gtcttaaaag agatttatga
     1621 tgtgccaaac tcgctctttc ataagcgcat ggactttttg aatgaagtct tggatttgaa
     1681 ggactttatc aaggatcccg tgcggactct ttcactggga caacggatgc gggcggatat
     1741 tgcggcctcc ttgctccaca atcccaaggt tcttttttta gatgagccga ccattggttt
     1801 ggacgtttcg gttaaggata atattcgtcg ggcaattact cagattaatc aagaggaaga
     1861 aactaccatt cttttgacca ctcacgattt gagtgatatt gagcaacttt gtgatcggat
     1921 tttcatgatt gacaaggggc aagagatttt tgatggaacg gtgagccaac tcaaggagac
     1981 ctttggtaag atgaagactc tctcttttga actgctacca ggtcaaagtc atctcgtctc
     2041 tcactatgaa ggtctgtctg atatgaccat tgatagacaa ggaaacagcc tcaacattga
     2101 atttgatagt tctcgctacc agtcagctga cattatcaag caaaccctgt ctgattttga
     2161 aatccgcgat ttgaagatgg tggatacgga tattgaggat attatccgtc gcttctaccg
     2221 aaaggagctc taggatgatc aaattgtgga gacgttataa accctttatc aatgcagggg
     2281 ttcaggagtt gattacttac cgagtcaact ttattctcta tcggattggc gatgtcatgg
     2341 gggcttttgt ggccttttat ctctggaagg ctgtctttga ttcttcgcaa gagtctttga
     2401 ttcagggctt cagtatggcg gatatcaccc tctacatcat catgagtttt gtgaccaatc
     2461 ttctgactag atccgattcg tcctttatga ttggggagga ggtcaaggat ggctccatta
     2521 tcatgcgttt gttgcgacca gtgcattttg cggcctccta tcttttcacc gagcttggtt
     2581 ccaagtggtt gatttttatc agcgttggcc ttccgttttt aagtgtcatt gtcttgatga
     2641 aaatcatatc gggtcaaggt attgtagagg tgctaggatt aactgtcctt tatcttttta
     2701 gcttaacgct cgcctatctg attaactttt tctttaatat ttgctttgga ttttcagcct
     2761 ttgtgtttaa aaatctttgg ggttccaacc tacttaagac ttccatagtg gcttttatgt
     2821 cggggagttt gattcccttg gcattctttc caaaggttgt ttcagatatt ctttcctttt
     2881 tgcctttttc atccttgatt tatactccag ttatgatcat tgttggaaaa tacgatgcca
     2941 gtcagattct tcaggcactc cttttgcagt tcttctggct cttagtgatg gtgggattgt
     3001 ctcagttgat ttggaaacgg gtccagtcct ttatcaccat tcaaggaggt tagtatgaaa
     3061 aaatatcaac gaatgcatct gatttttatc agacaataca tcaaacaaat catggaatat
     3121 aaggtggatt ttgtggttgg tgtcttggga gtctttctgg ctcaaggctt gaatctcttg
     3181 tttctcaatg tcatctttca acatattcca ttcctagaag gctggacctt tcaagagata
     3241 gctttcattt atggattttc cttgattccc aagggaatgg accatctctt ttttgacaat
     3301 ctctgggcac tagggcaacg cctagtccga aaaggggagt ttgacaagta tctgactcgt
     3361 cccatcaatc ctctctttca catcctagtt gaaacctttc agattgatgc cttgggtgaa
     3421 ctcttagtcg gtggtatttt attgggaaca acagtgacca gcattgtttg gactcttcca
     3481 aaattcctgc ttttcctagt ttgtattcct tttgcgacct tgatttatac ttctcttaaa
     3541 atcgcaacag ccagtatcgc cttttggact aagcagtcag gcgccatgat ttacatcttc
     3601 tatatgttca atgactttgc taagtatccg atttctattt acaattctct tcttcgttgg
     3661 ttgattagct ttatcgtgcc tttcgccttt acagcctact atccagctag ctatttctta
     3721 caggaaaagg atgtgttctt taacgtagga ggtttgatgt tgatttctct ggttttcttt
     3781 gttatttccc ttaaactttg ggataagggc ttagattcct acgaaagtgc gggttcgtaa
     3841 aagctaaagt aagactaaaa tcaagaaaga aacttatgat gtttgtaatt gaagaagtca
     3901 aggatgaaaa tcaaaaaaag acagttgtag ctgaggtttt gaaggatttg ccagaatggt
     3961 ttggaatccc agaaagcaca caagcctata tagaaggaac cacgacactg caagtttgga
     4021 ccgcctatca ggagagtgat ttgactggat ttgtaagctt atcctattcg agtgaagatt
     4081 gtgcagagat tgattgtctc ggcgtaaaaa agcttatcaa ggtagaaaaa ttgggagcca
     4141 attgcttgct actttagaga gtgaagctcg taaaaaagtt ggttatctgc aggtcaaaac
     4201 agtggcagaa ggttctaata aagattatga tcgaacaaat gacttttatc gaggtcttgg
     4261 ctttaaaaag ttagagattt tttctcagct atggaatccg caaaatcctt gtcagatttt
     4321 gattaaaaag cttgaataat attacttgac atctattctc agagtgccat actgtaagtg
     4381 taatcgccga tttagcttag ttggtagagc aaggcactcg taaagcctag gttataggta
     4441 gataaatgac tgaggagttg aaaaaataga taggtagaag ataaccgtta agccttattc
     4501 ttagcggtta tttatattgt ttaatagcgc tgatatttca tcaattatgc ctgttttcgt
     4561 gtttctggta gttgttcaag tttattgcta ctatttttga tggtatgaat gtgcttataa
     4621 tgtatcccgg ttaacgaaag ttttggactt atactcttag aaaatctctt caaaccacgt
     4681 caacgtcgcc ttgccgtgcg tatggttact gattacgtca gttctatcca caacctcaaa
     4741 acagtgtttt gagcaacct
//
LOCUS       DAVHEX010000095         4682 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVHEX010000095 DAVHEX010000000
VERSION     DAVHEX010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4682
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.096"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            129..656
                     /locus_tag="VVL45_002056"
     CDS             129..656
                     /locus_tag="VVL45_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU4188174.1"
                     /translation="MEDYCKEKNNISFKATFGDIKWSLLNKQAHRNTVSGDFGCLSNT
                     RKAQGRHLEQEGNIKHALIYYIESLIITISGLENNFSATDYPVYYPDSIPDYSLKHIQ
                     TLMESLSDDDYDFAFDEALFRFSILNANHFLSKEDIDYLRVNLPRSTAEEINNYLKKY
                     ECYSPLNNLELDDFE"
     gene            complement(823..894)
                     /locus_tag="VVL45_002057"
     tRNA            complement(823..894)
                     /locus_tag="VVL45_002057"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(859..861),aa:Arg,seq:cct)
     gene            complement(937..1284)
                     /gene="rplS"
                     /locus_tag="VVL45_002058"
     CDS             complement(937..1284)
                     /gene="rplS"
                     /locus_tag="VVL45_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEU4188175.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(1403..1732)
                     /gene="crcB"
                     /locus_tag="VVL45_002059"
     CDS             complement(1403..1732)
                     /gene="crcB"
                     /locus_tag="VVL45_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU4188176.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            complement(1726..2100)
                     /gene="crcB"
                     /locus_tag="VVL45_002060"
     CDS             complement(1726..2100)
                     /gene="crcB"
                     /locus_tag="VVL45_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU4188177.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIYLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYLSLI
                     LYLLLSIGGGLLLAYYLGRKKW"
     gene            complement(2097..2363)
                     /locus_tag="VVL45_002061"
     CDS             complement(2097..2363)
                     /locus_tag="VVL45_002061"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEU4188178.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREEVIFEKVRSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
     gene            complement(2479..2922)
                     /locus_tag="VVL45_002062"
     CDS             complement(2479..2922)
                     /locus_tag="VVL45_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEU4188179.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELAAKVG"
     gene            3026..3961
                     /locus_tag="VVL45_002063"
     CDS             3026..3961
                     /locus_tag="VVL45_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEU4188180.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQKILKQYNITDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKVHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            4015..4299
                     /locus_tag="VVL45_002064"
     CDS             4015..4299
                     /locus_tag="VVL45_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEU4188181.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(4356..4574)
                     /locus_tag="VVL45_002065"
     CDS             complement(4356..4574)
                     /locus_tag="VVL45_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188182.1"
                     /translation="MSISPRFETLEQAIASKDLEKVREAFKKMNSTWTINESVVRDNS
                     IAHYGRVETAISFLPSSMEIEPTDESGT"
     gene            complement(4571..>4682)
                     /locus_tag="VVL45_002066"
     CDS             complement(4571..>4682)
                     /locus_tag="VVL45_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEU4188183.1"
                     /translation="LEDVMNQLQDVIQGLEKEVIKSIGNRRWTRMLFESR"
BASE COUNT         1512 a          985 c          807 g         1378 t
ORIGIN      
        1 gaataattaa taatgttaac attgacaatt tagatattcc gttcgaattt aaactaacaa
       61 aagaagcaca aaatcttatt atcgaacata gtgactatat caaagcatac tatgataaag
      121 acataactat ggaagattat tgtaaagaaa aaaacaatat ctcttttaaa gcaacttttg
      181 gtgatataaa atggagtctc ttaaataaac aagctcatag gaatactgta tcaggagatt
      241 ttggatgctt atctaacaca cgaaaggctc agggaagaca tttggaacaa gaaggtaata
      301 ttaaacatgc tttaatatat tacatagaat ctttgataat tactatttca ggattagaaa
      361 acaatttttc agccactgat tatccagtat attatcccga ttcgatacct gactactcac
      421 taaaacatat tcaaacatta atggaatcat tatctgatga cgattatgat tttgcttttg
      481 atgaagcatt atttcgcttc tcaattttga atgcaaatca ttttttatct aaggaagata
      541 ttgactattt aagagttaat ttacctcgtt ccactgctga agaaataaac aattacttaa
      601 agaaatatga atgttatagt cctttaaata atttagaact tgacgatttt gaataaattg
      661 actatacaaa catttatata ctcgatatag tctcaatttt atctgatgat tgcccaaatt
      721 tttcaataat aaaacgcata atattatgga gacaatcccc tatattatgc gttcttttaa
      781 tatcaaagac tttttgacaa acttctttga tatctaatta catgccccct gcaggaatcg
      841 aacctgcaac tactccttag gagggagttg ttatatccat tgaactaagg gagctagata
      901 aaaactctgc taaatgagca gagtttttta gtcgaattaa cgacggattt ctttgatacg
      961 agctgcttta ccttgaagag cacgcaagta gtacaatttc gcacgacgta ctttaccgta
     1021 acgaacaact tcgatttttt caacacgtgg agtgtggatt gggaagatac gctcaacacc
     1081 tacaccgtta gagattttac gaactgtgta gttttctgag attccagcac ctttacgtgc
     1141 gataacaaca ccttcaaaaa tctggatacg ttcacggtta ccttcgacaa ctttcgcatg
     1201 tacacgaaca gtgtcaccag gacggaatga tgggatatct gtacgaagtt gaccttcagt
     1261 caagctttgg attaatggat tcattttatt ctcctatctt tgtcaatctt gaggaatctt
     1321 cctcagcgga taaactgtat ttttgtgcgt ccattacaca caagatatag tttaccaaat
     1381 ttctaaaaaa aagtaaagaa atttatagca aaattcctaa aaaaatcgca accaaaccac
     1441 ctatgtaagt caaagtcagg taagaataaa agaccttctt atcacttagc agtctttgaa
     1501 gttcgtcatt caaggtcgaa aaagttgtta aaccgccaca aaatcctgtt gctagaatag
     1561 catatacttc cttagactct acatgattgt agaatactcc aattaaaaaa caacctaaaa
     1621 gattagctat gagcgttccg agtggcaatt tagaagcttg attatagcga gaaaagaaat
     1681 accgtacgag tgctcccaag ccacaggcga ttgcaagata gacgattacc atttcttcct
     1741 ccccaaatag taagctaaaa gcaggcctcc accgatactc aaaagcaaat acagtatcaa
     1801 actaagataa cgccctgtat caagcagctt cacagtatca agcattaggc tggaaaaagt
     1861 tgttaaacct ccgcaaaaac ccgtccccag tgctaaaatc aagcccttac tagtcccctt
     1921 atagaccaga tagcccttga caagataaat caagcagaaa attcccagat agttgacaaa
     1981 gagagtgccc caagggaaat ctggactggc tggtaaccag gtggaaacta gatatcggac
     2041 aagtccgccc aacatagcag ctagaaaaat tcctagcgga taaaattgtt cttttttcat
     2101 ttgatatttt gatcctgata atcacgcgaa cgtttgagta tgtccgaaaa agtcgcgaca
     2161 atagtctcct gatagcgctt gtcctctaca cgactcctaa ccttttcaaa aataacttct
     2221 tctctcttag tatctaaaat cggtttacct gaagctttct tataagcaac caccccctca
     2281 actaaatgca ttcgttcttc taacagtttg acaatttggt cgtcgatttg atcgatttct
     2341 tgacgaataa tatctaaatc catacagtct cctcctttat ttgaattatt gtatcaaaaa
     2401 gccatcaatt aggctagtaa aatcccaact ctcgataaga aaaatgcact gattgattac
     2461 atcagcgcac ttttatgttt atcctacttt agcagccaat tcttctgcga attgttccaa
     2521 gcgttcaata tcttcttcct cggcagaaag atcaacttta acacactctg aacctttttc
     2581 tgctcctgtt gacacaaaga cacggtcaaa gtcatcaaca gccttacaga attcatcgta
     2641 gaaggtatct cctgagccga ccactccgta gattttgcca ttcaagttga gatctgctag
     2701 gtcttcgtag aagtccatca tctcatctgg caattcacca tcaccataag tataggtcgc
     2761 aacgatagcg atgtctgctt ccaagaagtc tgaagcgtca acagttgtac attcatcaac
     2821 atcaacatcc aagcccaagt cacgtaattt gtctgctaca atatctgcaa tttcttcggt
     2881 attaccggtc atactggcaa atacaatttt tgctaatgcc atatcgtcct cctcaattta
     2941 tctttctcca ttatatcaca tctttttcat tttaaaaata aaaattcttg tgctacaatg
     3001 aaatgagaaa gaattgaggt tatttatgga gatttgccaa caaattttag aaaaaattaa
     3061 agaatacgac accattatca ttcatcgtca tatgaaacca gaccctgatg ccttgggaag
     3121 tcaggtggga ttgaaagcct tgctggaaca tcatttccca gaaaaaacca tcaaagccgt
     3181 cggttttgat gaaccaactc ttacttggat ggctgagatg gatcttgttg aagatagagc
     3241 ctaccaaggc gcacttgtca tcgtctgtga tacagctaat actgctcgta tcgatgataa
     3301 gcgctatagt caaggtgatt ttctcattaa gattgaccac catccaaatg atgatgtata
     3361 cggtgacctg tcttgggtcg atactagttc aagtagcgct agcgagatga ttaccctatt
     3421 tgcccaaaca acccaactag ccttggcaga tcgcgatgct gagttgctct ttgcaggaat
     3481 tgtcggtgat acaggtcgct tcctctaccc ttctaccact gcacggactc ttcgcctggc
     3541 tgcttatttg agagaacata actttgactt tgcggctctc actcgcaaaa tggacactat
     3601 gagctacaaa attgctaaac tgcaaggcta catctacgac catctggaag tggatgaaaa
     3661 tggtgctgct cgcgttatcc tgagtcagaa aatcttgaaa caatacaata taaccgatgc
     3721 tgaaactgcg gccattgtag gtgcacctgg acgcattgac agagtgagtc tctggggaat
     3781 ttttgtcgaa caggctgatg gccactaccg agttcgctta cgcagtaaag tccatcctat
     3841 caatgaaatt gccaaggagc atgatggtgg aggccaccct ctagcaagtg gtgctaattc
     3901 ctatagccta gaagaaaacg aaatcatcta ccaaaagtta aaaaacttgc ttaaaaactg
     3961 ataaaatact tgccaaactt ttcagaatct gatagactag tatagtaaca atctatggct
     4021 cgcaaagaga ccatggcaga aaggaaatat tgcaaaatga aaaaagatat ccatccagaa
     4081 tatcgcccag ttgtcttcat ggacacaact actggttacc aattccttag cggttcaaca
     4141 aaacgctcta acgaaacagt tgagttcgaa ggcgaaactt acccattgat ccgtgtggaa
     4201 atttcatcag actcacaccc attctacact ggacgtcaaa agttcactca agcagatgga
     4261 cgcgtggatc gtttcaacaa aaaatacggt ctcaaataat gataagagaa cagttttggc
     4321 tgttcttttt tgtttcttga aatcaactgc tgttttcatg ttccagactc atctgtaggt
     4381 tcgatttcca tgctactagg caggaaggaa atagctgttt caacacgtcc ataatgagct
     4441 atactattgt cacgaaccac actttcattg atggtccaag tggaattcat tttcttaaaa
     4501 gcttctcgga ctttttccaa atctttggag gcaatggcct gctctaaggt ttcaaaacga
     4561 ggacttatac tcatctgctt tcaaaaagca ttctagtcca tctccgatta ccgatggact
     4621 ttatcacctc cttctccagt ccttgtatga catcttgaag ttgattcatg acatcttcca
     4681 aa
//
LOCUS       DAVHEX010000096         4267 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVHEX010000096 DAVHEX010000000
VERSION     DAVHEX010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.097"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            31..840
                     /locus_tag="VVL45_002067"
     CDS             31..840
                     /locus_tag="VVL45_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEU4188184.1"
                     /translation="MLLQKELIPMIEANLPNMAYAEKDIAKFFLKQQPLNDYSSKALC
                     EYLNVSKATLTRFAKKCGFKGFRQFIFKYQEMIHEKEKLALYTEATEKVLSDYEEMLR
                     KTYTVLDEVQLERIAEMIETAERVYLYGKGSSVLALQEMKMRFMRLGVIGEVLSDEDM
                     ILWSSLLLNENCLVIGASISGQTDIVLEGLQKAADKGAKTVLMTTRKFDEEDCFFDEL
                     LLLASTDHLSYGNRISPQFPILLITDCLFSNYLESPERQYYYNQTIIHKEE"
     gene            845..1546
                     /locus_tag="VVL45_002068"
     CDS             845..1546
                     /locus_tag="VVL45_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEU4188185.1"
                     /translation="MSQIWTKEKFISQVQGGVIVSCQALPGEALYNEEFSLMPFMAKA
                     ALEAGAVGIRANSVRDIKAIQKVVDLPIIGIIKRDYPPQEPYITATMKEVDELVECGT
                     TVIAFDATLRPRYDGLVVSEFIKKIKEKYPNQLLMADVSNLDEGLYAFKSGVDFVGTT
                     LSGYTSTSVQSDEPDFELMKKLADFNIPVIAEGKIHYPEQLKKAYSLGVTSVVIGGAI
                     TRPKEIAQRFINVIK"
     gene            1571..2488
                     /locus_tag="VVL45_002069"
     CDS             1571..2488
                     /locus_tag="VVL45_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HEU4188186.1"
                     /translation="MKDLTKYKGVIPAFYACYDENGEISQDRVKSLVQYFIDKGVKGI
                     YVNGSSGECIYQSVEDRKQIIEAVMEVAKGKLTVINHIACNNTKDSIELAKHSESVGV
                     DAIAAIPPIYFKLPEYSIAAYWNAMSEAASNTDFIIYNIPQLAGVALTGSLYATMRQN
                     PRVIGVKNSSMPVQDIQMFVAAGGEDYIVFNGPDEQYLGGRLMGAEAGIGGTYGVMPD
                     LFLKLESLIQERDLDTAKKLQYAINEVIYKMISGKANMYAVAKEVLRLNEKLDLGSVR
                     QPLEALAEGDLEVAKQAAELIQQARKEFL"
     gene            2516..4057
                     /locus_tag="VVL45_002070"
     CDS             2516..4057
                     /locus_tag="VVL45_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEU4188187.1"
                     /translation="MGTTGFTIIDLIILIVYLLAVLVAGIYFSKKEMKGKEFFKGDGS
                     VPWYVTSVSIFATMLSPISFLGLAGSSYAGSWILWFAQLGMVVAIPLTIRFILPIFAR
                     IDIDTAYDYLDKRFNSKALRIISALLFIIYQLGRMSIIMYLPSAGLSVLTGIDINILI
                     ILMGVVAIVYSYTGGLKSVLWTDFIQGVILISGVVLALFVLIANIKGGFGAVAETLAN
                     GKFLAANEKLFDPNLLSNSIFLIVMGSGFTILSSYASSQDLVQRFTTTQNIKKLNKML
                     FTNGVLSLATATVFYLIGTGLYVFYQVQNADSAASNIPQDQIFMYFIAYQLPVGITGL
                     ILAAIYAASQSTISTGLNSVATSWTLDIQDVISKNMSDNRRTKIAQFVSLAVGLFSIG
                     VSIVMAHSDIKSAYEWFNSFMGLVLGLLGGVFILGFVSKKANKQGAYAALIVSTIVMV
                     FIKYFLPPTAVSYWAYSLISISVSVVSGYIVSVLTGNKVSAPKYTTIHDITEIKADSS
                     WEVRH"
     gene            4076..>4267
                     /locus_tag="VVL45_002071"
     CDS             4076..>4267
                     /locus_tag="VVL45_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU4188188.1"
                     /translation="MIISEQSDFKRYASVNKYFSKVCDFLENTNLTDLVDGKIDIDGE
                     NVFANCMTYLADGVPGDIFE"
BASE COUNT         1388 a          647 c          829 g         1403 t
ORIGIN      
        1 gtaaacgttt acttcaacta aggagctcat atgttactgc aaaaagaact aattccaatg
       61 atagaagcta acttaccaaa tatggcatat gctgaaaaag acattgctaa attcttctta
      121 aaacagcaac ctctgaatga ttattcatct aaagcattgt gcgaatacct taatgtatcc
      181 aaagcaacat tgactcgatt tgcgaaaaaa tgtggtttta aaggttttag acaattcatt
      241 ttcaaatacc aagagatgat tcatgagaaa gaaaagttgg cattatatac agaggcaaca
      301 gaaaaagttt tatccgacta tgaggaaatg ttgagaaaaa cttacacggt tcttgatgaa
      361 gttcaacttg agcgtattgc tgagatgata gaaactgctg agcgtgtata tctctacggt
      421 aaaggaagtt ctgttcttgc tttacaagaa atgaagatga gatttatgcg tctcggagtg
      481 attggtgaag tattatcaga cgaggatatg attttgtgga gtagcttact acttaatgaa
      541 aattgccttg tcattggagc atccatttca ggtcaaactg atattgtact agaaggtcta
      601 caaaaagctg cagataaagg cgctaaaaca gttttaatga ctacaagaaa atttgacgaa
      661 gaagattgtt tctttgatga actattgtta ttagcttcga ccgatcatct ctcgtatggc
      721 aatcgcatat cacctcagtt tccaatactt ttaattacag actgcttatt ctctaattat
      781 ctggaaagtc cagagagaca atattattac aatcaaacta ttatccataa ggaggaataa
      841 taaaatgagc caaatttgga ctaaagaaaa atttataagc caagttcaag gtggagtcat
      901 tgtttcttgt caagctttac ctggtgaagc cctttataat gaagaattta gcttgatgcc
      961 ttttatggct aaagcagctt tagaggcagg agcagtgggc attcgcgcaa attctgtgcg
     1021 tgatattaaa gcaattcaga aagtagtaga tttaccaata attggaatta tcaaaaggga
     1081 ttatccacct caagaaccat atattactgc tacgatgaaa gaagtagatg aacttgtaga
     1141 atgcggaaca acagtcattg catttgatgc aactttaaga ccaagatatg atggcttagt
     1201 tgtcagtgaa tttatcaaaa aaataaaaga aaaatatccg aatcaattgc tgatggcgga
     1261 tgtaagtaat ttagatgaag gtctctatgc atttaaatca ggcgttgatt ttgttggtac
     1321 aacattatca ggttacacaa gtacaagtgt acaatcagat gagcctgatt ttgaactaat
     1381 gaaaaaattg gctgatttta atattccggt aattgccgaa ggaaaaattc attatccaga
     1441 acaattaaaa aaagcttata gtttaggtgt taccagtgta gtcattggtg gagcgattac
     1501 acgtccaaaa gaaattgctc agcgatttat taatgtcatc aaataaaaaa caatataaag
     1561 gagaaatctg atgaaagatt taactaaata caaaggcgtt atccctgcat tttatgcttg
     1621 ctatgatgaa aatggtgaaa ttagccaaga tcgtgtaaaa tctctggtac aatatttcat
     1681 tgacaaaggt gtaaaaggta tctatgtaaa tggttcttca ggtgaatgta tttaccaaag
     1741 tgtagaagat cgtaaacaaa ttattgaagc tgttatggaa gttgctaaag gtaaattaac
     1801 agttatcaac catattgcat gtaataacac gaaagatagt atcgaattgg caaaacattc
     1861 agaaagtgtt ggagtcgatg ctattgcagc tatcccacct atttatttca aattgccaga
     1921 gtattcaatc gcagcatatt ggaatgcaat gagtgaagct gcgtcaaata cagattttat
     1981 tatctataat attccacaat tggcaggggt tgcgttgact ggtagtttgt atgcaacaat
     2041 gcgtcaaaat cctcgtgtga ttggagttaa aaattcttct atgcctgtac aagatattca
     2101 aatgtttgta gctgcaggtg gagaagatta cattgtattc aatggtccag atgaacaata
     2161 tcttggtggt cgcttgatgg gagcagaagc tggtattggt ggtacttatg gcgttatgcc
     2221 agatttgttc ttgaaattgg aaagtttgat tcaagaacga gatttagata cagctaaaaa
     2281 acttcaatat gctatcaatg aagttatcta taagatgata tcaggtaagg caaatatgta
     2341 tgctgtagca aaagaagttt tgcgtctaaa tgaaaaactt gatttaggtt ctgttcgtca
     2401 acctttagaa gcattggcag aaggtgactt ggaagttgca aaacaagcag cagaacttat
     2461 tcaacaagca cgaaaagaat ttttataata aataaaatca atatggagat atgttatggg
     2521 cacaacagga tttacaataa ttgacttaat tatcttgatt gtttatttac ttgcggtgtt
     2581 ggttgcaggt atctatttct ctaaaaaaga gatgaaagga aaagagttct ttaaaggaga
     2641 tggttcggtt ccttggtatg ttacttcggt atccattttt gccacaatgc tcagtccgat
     2701 ttccttcttg ggactcgctg gtagctctta tgcaggtagc tggattttat ggtttgctca
     2761 attagggatg gtagtagcta ttccactgac aattcgtttt atcttaccta tctttgcacg
     2821 gatagacatc gatacggcat atgattactt ggataaacgt tttaattcta aagcacttcg
     2881 tattatttca gcactcttgt ttattattta tcaattggga cgtatgtcta tcattatgta
     2941 cctcccatca gctggtttat cagtattgac aggaattgac atcaatattt tgattatttt
     3001 gatgggtgta gttgcaattg tttattctta tactggtggt ctaaaatccg tattatggac
     3061 agactttatt caaggtgtga ttctgattag tggtgtcgtt ttagctttat ttgtactgat
     3121 tgctaatatt aaaggtggct ttggtgcagt agcagaaaca ttagcaaacg ggaaattcct
     3181 tgctgcaaat gaaaaacttt tcgatcctaa cttgctttca aactccatct ttttaattgt
     3241 gatgggttca ggctttacaa tcttgtcttc ctatgcttca tctcaagatt tggttcaacg
     3301 ttttactaca acacaaaata ttaagaaact taataagatg ttgttcacaa acggtgtttt
     3361 gtcacttgca actgcaacag tcttttactt gattggtaca ggcttgtacg tattctatca
     3421 agtacaaaat gcagatagtg cagctagcaa tatccctcaa gaccaaatct ttatgtactt
     3481 tattgcatac cagttaccag taggtatcac aggtttgatc ttggcagcga tttatgcagc
     3541 atctcaatca actatttcaa caggtttgaa ctctgttgca acttcatgga cattggatat
     3601 tcaagatgtc atttctaaaa atatgtcaga caatcgtcgt acgaaaattg cacaattcgt
     3661 atctctagca gtaggtttat tctcaattgg tgtttccatt gtcatggctc actcagatat
     3721 taaatctgca tacgaatggt tcaatagttt catgggactt gtacttggtc tacttggtgg
     3781 tgtatttatt cttggatttg tttctaaaaa agcaaataaa caaggtgctt atgcagcgct
     3841 gattgtatca accatcgtca tggtatttat taaatacttc cttcctccaa cagctgttag
     3901 ctactgggca tattcattga tttcaatctc tgtatcagta gtttcaggtt atattgtatc
     3961 tgttcttact ggaaataaag tatctgcacc taaatataca acgattcatg atattacaga
     4021 aattaaagcg gattcaagtt gggaagttcg tcactaatag aaaagagaaa ataatatgat
     4081 tatttctgaa caatctgatt ttaaacgata tgcttctgtc aataaatatt tttcaaaagt
     4141 ctgtgatttt ttagaaaata caaatttgac agatttagtt gatggaaaaa ttgatattga
     4201 cggggaaaat gtttttgcaa attgtatgac ttatctagct gatggagttc caggagatat
     4261 ttttgaa
//
LOCUS       DAVHEX010000097         3853 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVHEX010000097 DAVHEX010000000
VERSION     DAVHEX010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.098"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..418
                     /locus_tag="VVL45_002072"
     CDS             <1..418
                     /locus_tag="VVL45_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEU4188189.1"
                     /translation="KNKVDQDSKPDEDKEHDEVSEPTHPESDEKENHAGLNPSADNLY
                     KPSTDTEETEEEAEDTTDEAEIPQVENSVINAKIADAEALLEKVTDPSIRQNAMETLT
                     GLKSSLLLGTKDNNTISAEVDSLLALLKESQPAPIQ"
     gene            628..3747
                     /gene="phtE"
                     /locus_tag="VVL45_002073"
     CDS             628..3747
                     /gene="phtE"
                     /locus_tag="VVL45_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HEU4188190.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENLKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
BASE COUNT         1445 a          626 c          751 g         1031 t
ORIGIN      
        1 taaaaataag gtagaccaag acagtaaacc tgatgaagat aaggaacatg atgaagtaag
       61 tgagccaact caccctgaat ctgatgaaaa agagaatcac gctggtttaa atccttcagc
      121 agataatctt tataaaccaa gcactgatac ggaagagaca gaggaagaag ctgaagatac
      181 cacagatgag gctgaaattc ctcaagtaga gaattctgtt attaacgcta agatagcaga
      241 tgcggaggcc ttgctagaaa aagtaacaga tcctagtatt agacaaaatg ctatggagac
      301 attgactggt ctaaaaagta gtcttcttct cggaacgaaa gataataaca ctatttcagc
      361 agaagtagat agtctcttgg ctttgttaaa agaaagtcaa ccggctccta tacagtagta
      421 aaatgaatgg agcatatttt atggagaagt aacctttcgt gttacttctc ttttttagaa
      481 aaacgtaaca gaaacttgac aaaagtaatt ttaaatagta aactatttac tggttaatta
      541 aatggttaaa taaccggttt agaaaactat ttaataaagt aaaagaagtt gagaaaaaac
      601 ttcatcattt attgaaatga gggatttatg aaatttagta aaaaatatat agcagctgga
      661 tcagctgtta tcgtatcctt gagtctatgt gcctatgcac taaaccagca tcgttcgcag
      721 gaaaataagg acaataatcg tgtctcttat gtggatggca gccagtcaag tcagaaaagt
      781 gaaaacttga caccagacca ggttagccag aaagaaggaa ttcaggctga gcaaattgta
      841 atcaaaatta cagatcaggg ctatgtaacg tcacacggtg accactatca ttactataat
      901 gggaaagttc cttatgatgc cctctttagt gaagaactct tgatgaagga tccaaactat
      961 caacttaaag acgctgatat tgtcaatgaa gtcaagggtg gttatatcat caaggtcgat
     1021 ggaaaatatt atgtctacct gaaagatgca gctcatgctg ataatgttcg aactaaagat
     1081 gaaatcaatc gtcaaaaaca agaacatgtc aaagataatg agaaggttaa ctctaatgtt
     1141 gctgtagcaa ggtctcaggg acgatatacg acaaatgatg gttatgtctt taatccagct
     1201 gatattatcg aagatacggg taatgcttat atcgttcctc atggaggtca ctatcactac
     1261 attcccaaaa gcgatttatc tgctagtgaa ttagcagcag ctaaagcaca tctggctgga
     1321 aaaaatatgc aaccgagtca gttaagctat tcttcaacag ctagtgacaa taacacgcaa
     1381 tctgtagcaa aaggatcaac tagcaagcca gcaaataaat ctgaaaatct ccagagtctt
     1441 ttgaaggaac tctatgattc acctagcgcc caacgttaca gtgaatcaga tggcctggtc
     1501 tttgaccctg ctaagattat cagtcgtaca ccaaatggag ttgcgattcc gcatggcgac
     1561 cattaccact ttattcctta cagcaagctt tctgccttag aagaaaagat tgccagaatg
     1621 gtgcctatca gtggaactgg ttctacagtt tctacaaatg caaaacctaa tgaagtagtg
     1681 tctagtctag gcagtctttc aagcaatcct tcttctttaa cgacaagtaa ggagctctct
     1741 tcagcatctg atggttatat ttttaatcca aaagatatcg ttgaagaaac ggctacagct
     1801 tatattgtaa gacatggtga tcatttccat tacattccaa aatcaaatca aattgggcaa
     1861 ccgactcttc caaacaatag tctagcaaca ccttctccat ctcttccaat caatccagga
     1921 acttcacatg agaaacatga agaagatgga tacggatttg atgctaatcg tattatcgct
     1981 gaagatgaat caggttttgt catgagtcac ggagaccaca atcattattt cttcaagaag
     2041 gacttgacag aagagcaaat taaggctgcg caaaaacatt tagaggaagt taaaactagt
     2101 cataatggat tagattcttt gtcatctcat gaacaggatt atccaagtaa tgccaaagaa
     2161 atgaaagatt tagataaaaa aatcgaagaa aaaattgctg gcattatgaa acaatatggt
     2221 gtcaaacgtg aaagtattgt cgtgaataaa gaaaaaaatg cgattattta tccgcatgga
     2281 gatcaccatc atgcagatcc gattgatgaa cataaaccgg ttggaattgg tcattctcac
     2341 agtaactatg aactgtttaa acccgaagaa ggagttgcta aaaaagaagg gaataaagtt
     2401 tatactggag aagaattaac gaatgttgtt aatttgttaa aaaatagtac gtttaataat
     2461 caaaacttta ctctagccaa tggtcaaaaa cgcgtttctt ttagttttcc gcctgaattg
     2521 gagaaaaaat taggtatcaa tatgctagta aaattaataa caccagatgg aaaagtattg
     2581 gagaaagtat ctggtaaagt atttggagaa ggagtaggga atattgcaaa ctttgaatta
     2641 gatcaacctt atttaccagg acaaacattt aagtatacta tcgcttcaaa agattatcca
     2701 gaagtaagtt atgatggtac atttacagtt ccaacctctt tagcttacaa aatggccagt
     2761 caaacgattt tctatccttt ccatgcaggg gatacttatt taagagtgaa ccctcaattt
     2821 gcagtgccta aaggaactga tgctttagtc agagtgtttg atgaatttca tggaaatgct
     2881 tatttagaaa ataactataa agttggtgaa atcaaattac cgattccgaa attaaaccaa
     2941 ggaacaacca gaacggccgg aaataaaatt cctgtaacct tcatggcaaa tgcttatttg
     3001 gacaatcaat cgacttatat tgtggaagta cctatcttgg aaaaagaaaa tcaaactgat
     3061 aaaccaagta ttctaccaca atttaaaagg aataaagcac aagaaaactt aaaacttgat
     3121 gaaaaggtag aagaaccaaa gactagtgag aaggtagaaa aagaaaaact ttctgaaact
     3181 gggaatagta ctagtaattc aacgttagaa gaagttccta cagtggatcc tgtacaagaa
     3241 aaagtagcaa aatttgctga aagttatggg atgaagctag aaaatgtctt gtttaatatg
     3301 gacggaacaa ttgaattata tttaccatcg ggagaagtca ttaaaaagaa tatggcagat
     3361 tttacaggag aagcacctca aggaaatggt gaaaataaac catctgaaaa tggaaaagta
     3421 tctactggaa cagttgagaa ccaaccaaca gaaaataaac cagcagattc tttaccagag
     3481 gcaccaaacg aaaaacctgt aaaaccagaa aactcaacgg ataatggaat gttgaatcca
     3541 gaagggaatg tggggagtga ccctatgtta gatccagcat tagaggaagc tccagcagta
     3601 gatcctgtac aagaaaaatt agaaaaattt acagctagtt acggattagg cttagatagt
     3661 gttatattca atatggatgg aacgattgaa ttaagattgc caagtggaga agtgataaaa
     3721 aagaatttat ctgatctcat agcgtaagga atagcagtag aaaaagtctg aatcaaaaat
     3781 gaagttctct caaaagttag aaataaaact ctgactttgg gagaatttca ttttattatt
     3841 aatatataaa att
//
LOCUS       DAVHEX010000098         3592 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVHEX010000098 DAVHEX010000000
VERSION     DAVHEX010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.099"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            148..375
                     /locus_tag="VVL45_002074"
     CDS             148..375
                     /locus_tag="VVL45_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188191.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            663..3560
                     /gene="nanA"
                     /locus_tag="VVL45_002075"
     CDS             663..3560
                     /gene="nanA"
                     /locus_tag="VVL45_002075"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HEU4188192.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGESSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVSS
                     ATKKDEYFTMAVYNNTATLEGRGSDGKQFYNNYNDAPLKVKPGQWNSVTFTVEKPTAE
                     LPNGRVRLYVNGVLSRTSLKSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAALTEKTDIFESGRNGKPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDRVTITNLRDNPKASD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKKIDGKTYQILY
                     REGEKGAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGNQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NRRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHNGKEYIILSNAGGPNRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWEFLSKNLISPTEAKVKRTREMGKGEMG
                     KGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKG
                     SIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLV
                     TLTTKKDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
BASE COUNT         1227 a          600 c          815 g          950 t
ORIGIN      
        1 tgaggtataa taaatttact aacgcttata aaaagtgata gaatctattt ttatgtatat
       61 ttaaagatag attgctgtaa aaatagtagt agctatgcga aataacagat agagagaagg
      121 gattgaagct tagaaaaggg gaataatatg atatttaagg cattcaagac aaaaaagcag
      181 agaaaaagac aagttgaact acttttgaca gtttttttcg acagttttct gattgattta
      241 tttcttcact tatttgggat tgtccccttt aagctggata agattctgat tgtgagcttg
      301 attatatttc ccattatttc tacaagtatt tatgcttatg aaaagctatt tgaaaaagtg
      361 ttcgataagg attgagcagg aagtatggtg taaatagcat aggctgatgt ccatcatttg
      421 cttataaaga gatattttag tttaattgca gcggtgtcct ggtagataaa ctagattggc
      481 aggagtctga ttggagaaag gagaggggaa aattggcacc aatttgagat agtttgttta
      541 gttcattttt gtcatttaaa tgaactgtag taaaagaaag ttaataaaag acaaactaag
      601 tgcattttct ggagtaaatg tcttatttca gaaatcggga tatagatata gagaggatca
      661 gtatgaatcg gagtgttcaa gaacgtaagt gtcgttatag cattaggaaa ctatcggtag
      721 gagcggtttc tatgattgta ggagcagtgg tatttggaac gtctcctgtt ttagctcaag
      781 aaggggcaag tgagcaacct ctggcaaatg aaactcaact ttcgggggag agctcaaccc
      841 taactgatac agaaaagagc cagccttctt cagagactga actttctggc aataagcaag
      901 aacaagaaag gaaagataag caagaagaaa aaattccaag agattactat gcacgagatt
      961 tggaaaatgt cgaaacagtg atagaaaaag aagatgttga aaccaatgct tcaaatggtc
     1021 agagagttga tttatcaagt gaactagata aactaaagaa acttgaaaac gcaacagttc
     1081 acatggagtt taagccagat gccaaggctc cagcattcta taatctcttt tctgtgtcaa
     1141 gtgctactaa aaaagatgag tacttcacta tggcagttta caataatact gctactctag
     1201 aggggcgtgg ttcggatggg aaacagtttt acaataatta caacgatgca cccttaaaag
     1261 ttaaaccagg tcagtggaat tctgtgactt tcacagttga aaaaccgaca gcagaattgc
     1321 caaatggtcg agttcgcctg tatgttaatg gtgttttatc tcgaacaagt ctgaaatctg
     1381 gcaatttcat taaagatatg ccagatgtaa cgcatgtgca aatcggagca accaagcgtg
     1441 ccaacaatac ggtttggggg tcaaatctac agattcggaa tctcactgtg tataatcgtg
     1501 ctttaacacc agaagaggta caaaaacgta gtcaactttt taaacgctca gatttagaaa
     1561 aaaaactacc tgaaggagcg gctttaacag agaaaacgga catattcgaa agcgggcgta
     1621 acggtaaacc aaataaagat ggaatcaaga gttatcgtat tccagcactt ctcaagacag
     1681 ataaaggaac tttgatcgca ggtgcagatg aacgccgtct ccattcgagt gactggggtg
     1741 atatcggtat ggtcatcaga cgtagtgaag ataatggtaa aacttggggt gaccgagtaa
     1801 ccattaccaa cttacgtgac aatccaaaag cttctgaccc atcgatcggt tcaccagtga
     1861 atatcgatat ggtgttggtt caagatcctg aaaccaaacg aatcttttct atctatgaca
     1921 tgttcccaga agggaaggga atctttggaa tgtcttcaca aaaagaagaa gcctacaaaa
     1981 aaatcgatgg aaaaacctat caaatcctct atcgtgaagg agaaaaggga gcttatacca
     2041 ttcgagaaaa tggtactgtc tatacaccag atggtaaggc gacagactat cgcgttgttg
     2101 tagatcctgt taaaccagcc tatagcgaca agggtgatct atacaagggt aaccaattac
     2161 taggcaatat ctacttcaca acaaacaaaa cttctccatt tagaattgcc aaggatagct
     2221 atctatggat gtcctacagt gatgacgacg ggaagacatg gtcagcgcct caagatatta
     2281 ctccgatggt caaagccgat tggatgaaat tcttgggtgt aggtcctgga acaggaattg
     2341 tacttcggaa tgggcctcac aagggacgga ttttgatacc agtttatacg actaataatg
     2401 tatctcactt aaatggctcg caatcttctc gtgtcatcta ttcagatgat catggaaaaa
     2461 cttggcatgc tggagaagcg gtcaacgata accgtcaggt agacggtcaa aagatccact
     2521 cttctacgat gaacaataga cgtgcgcaaa atacagaatc aacggtggta caactaaaca
     2581 atggagatgt taaactcttt atgcgtggtt tgactggaga tcttcaggtt gctacaagta
     2641 aagacggagg agtgacttgg gagaaggata tcaaacgtta tccacaggtt aaagatgtct
     2701 atgttcaaat gtctgctatc catacgatgc ataatggaaa agaatacatt atcctcagca
     2761 atgcaggtgg accgaatcgt gaaaatggga tggtccactt ggcacgtgtc gaagaaaatg
     2821 gtgagttgac ttggctcaaa cacaatccaa ttcaaaaagg agagtttgcc tataattcgc
     2881 tccaagaatt aggaaatggg gagtatggca tcttgtatga acatactgaa aaaggacaaa
     2941 atgcctatac cctatcattt agaaaattta attgggaatt tttgagcaaa aatctgattt
     3001 ctcctaccga agcgaaagtg aagcgaacta gagagatggg caaaggagag atgggcaaag
     3061 gagttattgg cttggagttc gactcagaag tattggtcaa caaggctcca acccttcaat
     3121 tggcaaatgg taaaacagcg actttcctaa cccagtatga tagcaagacc ttgttgtttg
     3181 cagtagataa ggaagatatc ggacaggaaa ttattggtat agctaaagga agcatcgaaa
     3241 gtatgcataa tcttcctgta aatctagcag gtgccagagt tcctggcgga gtaaatggta
     3301 gcaaagcagc ggtgcatgaa gttccagaat ttacaggggg agttaatggt acagagccag
     3361 ctgttcatga aatcgcagag tataagggat ctgattcgct tgtaactctt actacaaaaa
     3421 aagattatac ttacaaagct cctcttgctc agcaggcact tcctgaaaca ggaaacaagg
     3481 agagtgacct cctagcttca ctaggactaa cagctttctt ccttggtctg tttacgctag
     3541 ggaaaaagag agaacaataa gagaagaatt ctaaacattt gattttgtaa aa
//
LOCUS       DAVHEX010000099         3559 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVHEX010000099 DAVHEX010000000
VERSION     DAVHEX010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.100"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..182
                     /locus_tag="VVL45_002076"
     CDS             <1..182
                     /locus_tag="VVL45_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU4188193.1"
                     /translation="YENRTVSFAISSKGEKMKVENISYRVDHRKLFDNISFDTSSSDV
                     TLITGKNGTGKSTLL"
     gene            complement(548..1858)
                     /locus_tag="VVL45_002077"
     CDS             complement(548..1858)
                     /locus_tag="VVL45_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEU4188194.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            2095..2967
                     /locus_tag="VVL45_002078"
     CDS             2095..2967
                     /locus_tag="VVL45_002078"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEU4188195.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAILLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
     gene            3098..3559
                     /locus_tag="VVL45_002079"
                     /pseudo
     CDS             3098..3559
                     /locus_tag="VVL45_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         1127 a          608 c          780 g         1044 t
ORIGIN      
        1 agtatgaaaa cagaactgtt tcttttgcta ttagttcaaa aggagaaaaa atgaaagtag
       61 aaaatatttc gtatagggtg gatcatcgta aattgtttga taatatttct tttgatactt
      121 cgagttcaga cgtgacatta attactggta aaaatggtac aggaaagtca actttactat
      181 agtagattga aactagaata gtacacatct acttctaaaa tattgttaga aatcgatttg
      241 actatcctga tctatttgtc ctgttcttat ttcatttcac tatatctcaa attgagtatg
      301 acgaagtgcg ctcccatgtc ctgggaacgc actttcttca tatttttcat attcttgaat
      361 ccatcgataa agactattgg gatgaatttt taaagttgaa ctaatcattt ttacaggatg
      421 agatttacag cagagagttt gaaggcttta tcaaaggttt ttcttggcat aatgactttt
      481 cctcgtttcc acttaatttt gtgtctactt tattatacca agtccaccct taagttagat
      541 aataaatcta acttaaggaa gctagaagga tgagaatcca ggtggtcaag agtcccaaac
      601 ttaagctgat ggggacaccc agaataattt gctttttgaa ggcaaggcca cgttcctcta
      661 tattgggaag tgagagttga atgagagaac cagctgatga aaagggtgag atattagtag
      721 atagagcgcc aataacggtg gctgttgtga gtaagtgaat atcaatctga ggattttgag
      781 cactgatgat agcaatgatg ggaaagaggg ctggagctac aacggatagg gtggaactaa
      841 agagtgacat cactccggct atcacacaaa agaacagagg taaccagaaa tgaggaatgg
      901 ttgttgtcat gaggtgccct atcagtgtga ctaaacctga cttgaccgct agagacatta
      961 gtaagctcat gccgcagagc atgataattg tagcccaggg aaccttagct aaaatggctt
     1021 cttgcttccc taatttgagc cttaaggcga ggcagaccat gagtattgag acaaagccaa
     1081 tatcaaatgt tttttgataa gtagctatcc aggcgatgtt tgggaaaatg agatgcaaca
     1141 agggaaaaag ccaaaccaaa accatgctgc tgatcatgag caaggtggtt tgtctttgaa
     1201 ccttgctgag gagtggtggt tggtcaatag tcaaggatga gtttgttctt cccttactat
     1261 agtgactgta acaggataat aaaagcaaga cgatgagtgg gtagataatg ctgacgataa
     1321 agatatgatt gccaagtgaa aaagcttgct cttcccatcc catttgctta aacaggcctt
     1381 gaaagacaat gcctgagcta ctggttatca aattagcccc tcctgaagct ccccaattga
     1441 cggcttgagc tccaatcaaa gggtgtttgt ccgctttttg acagagggta atcgctagag
     1501 gacagcaaac ggccatagta gtgaaaaatc cagcacctaa agcagacaaa agggttgcca
     1561 tcaggtataa aatcatgtag agggcgttag ggtgggtgcg tgtgcggtag agaatgtgtt
     1621 gagccaaaac atcaagagta ccgttagttg ttgcaacgtt ataaaagaga gagacgctaa
     1681 aaatggtaaa aaagagtgag gttggccaaa aatgaagaag ttctttgggg cttaatccca
     1741 tgagagtggt tgcgatgagg taagaaaaag caatagccag caggccaata ttgattttgg
     1801 tgcggtaacc aattccaatg gctagagcaa tggcgctaat cattattaaa tgaatcattg
     1861 aattgtcctt tcagttagaa tatggaaata ggttggcaat tttggcgctc aaaagaccac
     1921 ctgtttccta ttggtacttg actattatac acaaaaaaaa tacaattatc ttgacaattg
     1981 tattgacact tgttagaatt aaaaatgagg tattcaggct tgtgatttcc tatggaagtt
     2041 aatagtgatt gcctctaatg cttacaagtg atattaaaaa tagaggacct agtgatgtca
     2101 atcatttcaa ctgatttaac cccttttcaa atagatgata cattgaaagc agccttgcga
     2161 gaagatgttc attccgaaga ttacagtacc aatgccattt ttgatcatca tggccaagcc
     2221 aaggtgtcgc tttttgccaa ggaagctggt gttttagcgg ggctaaccgt ttttcaaagg
     2281 gtttttaccc tatttgatgc cgaggtgacc ttccagaatc ctcatcaatt taaggatggg
     2341 gatcgtttga ctagtggcga tttggtttta gaaatcatag gctcggtgag aagtctctta
     2401 acatgtgaac gcgttgcctt gaatttttta caacatttat cagggattgc ttcgatgaca
     2461 gctgcttatg tagaagcctt aggcgatgat tgcattaagg tatttgatac tcgaaaaact
     2521 actcctaatt tacgtctttt tgagaaatat gccgtgagag ttggcggtgg ctataatcat
     2581 cgctttaatt tatcagatgc tatcctgcta aaagacaatc acattgcggc agtaggtagt
     2641 gttcaaaagg caattgctca agcgcgtgcc tatgcccctt ttgtgaaaat ggtcgaggtg
     2701 gaagtggaaa gccttgctgc tgccgaagaa gctgcggcgg cgggtgctga tattatcatg
     2761 ttggataata tgtcattgga acagattgaa caggccatta ccctaattgc aggacgttct
     2821 cggattgaat gttctggaaa tattgatatg accactatta gccgttttcg tggtttagcg
     2881 attgattacg tctccagtgg tagtttaacc catagtgcta agagtcttga tttttccatg
     2941 aaggggttaa cctaccttga tgtctaagtt gtaaaataaa ctaacttttt aaaggatgtc
     3001 tttcctctag aacgagtttt atgtcagata gtttaaacgc ctcttcaaat atagtaaaat
     3061 gaaccaaaaa tagtacacaa tgtggtataa tctttttatg gcatattcaa tagattttcg
     3121 taaaaaagtt ctctcttatt gtgagcgaac aggtagtata acagaagcat cacacgtttt
     3181 ccaaatctca cgtaatacca tttatggctg gttaaagcta aaagagaaaa caggagagct
     3241 aaaccaccaa gtaaaaggaa taaaaccaag aaaggttgat agagatagac ttaaaaacta
     3301 tcttactgac aatccagacg cttatttgac tgaaatagct tctgaatttg gctgtcatcc
     3361 aactaccatc cactatgcgc tcaaagctat gggctacact cgaaaaaaag aaccacacct
     3421 actatgaaca agacccagaa aaagtagcct tatttcttaa gaattttaat agtttaaagc
     3481 acctaacacc tgtttagatt gacgaaacag gattcgatac ttatttttat cgagaatatg
     3541 gtcgctcatt aaaaggtca
//
LOCUS       DAVHEX010000100         3137 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVHEX010000100 DAVHEX010000000
VERSION     DAVHEX010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.101"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(60..131)
                     /locus_tag="VVL45_002080"
     tRNA            complement(60..131)
                     /locus_tag="VVL45_002080"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(96..98),aa:Glu,seq:ttc)
     gene            complement(193..1548)
                     /gene="rlmD"
                     /locus_tag="VVL45_002081"
     CDS             complement(193..1548)
                     /gene="rlmD"
                     /locus_tag="VVL45_002081"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU4188196.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
     gene            1586..2362
                     /gene="recX"
                     /locus_tag="VVL45_002082"
     CDS             1586..2362
                     /gene="recX"
                     /locus_tag="VVL45_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEU4188197.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDINENIVSQVIANLKEEKW
                     INDSQYAYAIINANQLSGDKGPYVLTQKLAQKGISKSTIEEILNDFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYFDAKIAFDELDSQVDEETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            2451..2984
                     /locus_tag="VVL45_002083"
     CDS             2451..2984
                     /locus_tag="VVL45_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEU4188198.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
BASE COUNT         1000 a          575 c          568 g          994 t
ORIGIN      
        1 gaagattttt aagttttttt aaactttttt tcatcaagtg gtcctgacgc aacataccat
       61 agtccgtacg ggattcgaac ccgtgttacc gccgtgaaaa ggcggtgtct taaccccttg
      121 accaacggac ctgagttgtt attttcaact cttactatta tacagtcttt tcaaactttg
      181 tcaactactt ttttaaactt tttttattaa ttttacaaca gcttcagttc gagctgtatg
      241 tgggaacata tcgaccgact ggatataatg aagatcatag acttctacta agcgtaccaa
      301 atcacgagcc aaggtcgaaa cattacaaga aatataaacc attttttctg gtacataagt
      361 aagaatagta tctaataact tatcatccag acctgtacgt ggtgggtcaa caatcaaagc
      421 atctgctcgg tagccttcct tgtaccaacg aggaataatc tcttctgccg ttccagcttc
      481 ataatgagta ttgtcaaatc ccattctttt agcatttcgc ttggcatctt caatagcttc
      541 tggaataata tccatacctc tgagtgtttt tactttcttt gcaaaggcaa atccaatcgt
      601 tccaactcca caataagcgt caatcaaatg gtcttcttta tcaacatcca gcgcttttac
      661 tgcttcgcta tagaggactt ctgtttgctc aggatttagt tgataaaaag ctcgagggga
      721 tagtgaaaat tcataattga gtacaccttc ttgaatactc tcttgccccc agataatctc
      781 tgtcttttca ccatatatct cactggtttt agctgtattt gtattaacag ctactgtcac
      841 aacttctggg aaatctttaa ccaactcttt taccaattga gttaaattaa gctggcggtt
      901 tgtaacaata ataatctgaa cctgtccggt ctttctcgcg cgtcggacca taatagtacg
      961 gacacctaga acttttctct catccgtgat tggaatctgg tgataagtaa gtaattctgc
     1021 taagcgatta gcaatcactt gggtttcctt atcttgtacc aggcagtctt tcaactctac
     1081 taaatagtga gagttttgtg catataagcc cgccttgacc tgatttttaa attttcgagt
     1141 ctgaaattgt aacttagctc tgtaatattt tggttcctgc attccaatag ttggacgaat
     1201 ttcataattt tcatatcctg caggagcaaa ttttttcagc gcttgatgaa gtaagtccgt
     1261 cttgaactcc agctgcttat cataatgcag gtgcatgatt tggcagcctc cgcattcatt
     1321 ataaatagta caagatggca caattcgaaa tttagacttc ttgttgacct tcagtaattt
     1381 tgcttcaaca aagttgcgtc taatagaagt aatctgacaa tagatatctt cgcctttgag
     1441 agctcctggt acaaagacta atgttttttg gtaaaagccg attccctcac cgttaattcc
     1501 catgcgcttg atttttaatg gtattttttg tttcactttc agattcatac ccctatctta
     1561 tcacattttg agttataata gagctatgaa aatcacaaaa cttgaaaaga aaaaaagact
     1621 ctatctgatg gagcttgata atggcgacaa atgctatatc accgaagaca caattgttcg
     1681 ttttatgtta tcgagagata aggtgataag cgaagaggaa ttgaaagaga ttcaagactt
     1741 tgctcaattt tcttatggta agaatctggc cctctaccac ttatccttta aagcacgtac
     1801 tgaaaaagag gtcagagaat atctgaaaaa atatgatatt aatgaaaaca tcgtttctca
     1861 agtcattgct aatcttaaag aagagaagtg gattaatgat agccagtacg cttatgctat
     1921 catcaatgcc aatcaacttt caggagacaa gggaccttat gtactgactc agaaattagc
     1981 acaaaaagga atttcaaaat ctactataga agaaatcttg aatgattttg atttttcgga
     2041 agttgctcaa cgtgtagcca ataaactatt gaaaaaatat gagggaaaac ttccagctcg
     2101 tgccctgcaa gataagatta tccagaactt gactaacaag ggcttctctt attttgatgc
     2161 taaaattgcc tttgacgaat tggatagtca agttgatgaa gaaacgactc aggaactcat
     2221 cttcaaggaa cttgataagc aatatactaa gtatgcccga aagtatgaag gatacgaact
     2281 gaagcagcgt ttaactcaag ttttagcacg aaagggctac gatttttcgg atatagcaag
     2341 cgctcttaga gaatatcttt aatatttcca agtgaaattc acagattttg ggtaatttta
     2401 tggtacaata gtaaatgata aacttataaa ttgtagaaag ttggttagtt atgaagcttc
     2461 caaaagaagg cgactttatt acaattcaaa gttataagca tgatgggagt ctccaccgaa
     2521 cttggcggga caccatggta ctaaaaacaa cagaaaacgc cattattggt gtcaacgatc
     2581 atacacttgt taccgaaagt gatggtcgtc gttgggtcac tcgagaaccg gctattgttt
     2641 actttcacaa gaaatattgg tttaatatca ttgccatgat tcgtgataat ggaacttctt
     2701 actattgcaa tatggctagc ccctactatc tggatgaaga agcactgaag tatattgatt
     2761 acgatttgga tgttaaaatt tttacagatg gggaaaaacg tctcttggac gttgaagagt
     2821 acgagcgtca caaacgcaaa atgaattatt ctgatgactt ggactatatt ttaaaagaac
     2881 atgtcaaaat tcttgttgat tggattaaca atggacgtgg tcctttctca gaagcctatg
     2941 taaacatttg gtacaagcgc tatgtagaac taaagaatcg gtaaagttgt caaaggtcag
     3001 gaacaaaatc ctgacctttt ttggtagtat aaaataaatt gtgtaaacac aaaaaggaat
     3061 aaatctatta tagcagagtt acaacacatt actagaaaga gatttatgca gaattcgtaa
     3121 aatatataaa acaaata
//
LOCUS       DAVHEX010000101         3108 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVHEX010000101 DAVHEX010000000
VERSION     DAVHEX010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.102"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            679..2838
                     /gene="pfbA"
                     /locus_tag="VVL45_002084"
     CDS             679..2838
                     /gene="pfbA"
                     /locus_tag="VVL45_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HEU4188199.1"
                     /translation="MLKIVKKLEVLMKYFVPNEVFSIRKLKVGTCSVLLAISILGSQG
                     ILSDEAVTSSSPMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNS
                     ISSNGTIRSNSQLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAV
                     GDGIHDDRQAIQDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILN
                     GINIKNHPSIVFMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLI
                     NSSGAFAIGNSNNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQ
                     IISKESIQIEPLTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTL
                     STQNPSNIKILNNHFDNMMYSGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVN
                     AYSYKNTKDLLDLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKVSDITVTKNVVN
                     NNSKETEQPNIELLRVSDNLVVSENSIFGGKEGIVIEDSKGKITVLNNQFYNLSGKYI
                     SFIKSNANGKEPVIRDSDGNFNIVTENGLYKIVTNNLSDKNEKEKNKEEKQSNSNNVI
                     DSNQKNGEFNSSKDNRQMNDKIDNKQDNKTEEVNYKIVGDGRETENHINKSKEIVDVK
                     QKLPKTGSNKIMELFLTVTGIGLLLTLKGLKYYGKDK"
     gene            2822..>3108
                     /locus_tag="VVL45_002085"
     CDS             2822..>3108
                     /locus_tag="VVL45_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188200.1"
                     /translation="MVKINKICSIQGSSVENEDIVGSQNQYFWIIGGATDLYNSKEEI
                     GYSVSEVVHILSESLSVNCKESKTLKQIFETALLEVKDEIGLNSYELTEYS"
BASE COUNT         1185 a          398 c          580 g          945 t
ORIGIN      
        1 cattagatct cccgcgagac aatctataaa acacttgtct acgattacct atatgcccta
       61 ttccagtatt ttagaagcac tgcatctatt tttatcgagg ttaaatctag cttttataga
      121 aggtctattt aagaaatata ttgtagtgtt ttagtttcaa tccgccatat gagcgatatt
      181 catgtaaata tccctggcga atgcttgtat gacaaggtat ttgttctttc atttataatt
      241 tacaacatat caacaaattt aaatatagta aatgggatat tttatattca agctaagaaa
      301 gatagcatca cttttgaatg gaaggctaaa gagcaaacta ggaagttggc catagatagc
      361 tcaaaaccct gctttgaggt tgtagatata gtaaaatgaa ataagaatag gacaaatcga
      421 tcgggacagt caaatcgatt tctaacaatg ttttagaagt agaggtgtac tattatagtt
      481 tccgtctact atagaactga ccaagtcagt aacctagact tagggcaagg cggcactgac
      541 ctagtttgaa gagatttccg aagagtataa attttaatat tttcttgtgt tattccttga
      601 caattcaatt tggaaaatat atgataaaga taatgacagc ggtgtcattc tatctatttt
      661 aagaaaagta ataatcaatt gttaaaaata gtaaaaaaat tggaggttct gatgaaatat
      721 tttgttccta atgaggtatt cagtattcgt aaattaaagg tagggacttg ctcggtacta
      781 ttggcaattt caattttggg aagccaaggt attttatcgg atgaagctgt tactagttct
      841 tcaccgatgg ctacaaaaga gtcttctaat gcaattacta atgatttaga taattcacca
      901 actgttaatc agaatcgttc tgctgaaatg attgcctcta attcaaccac taatggttta
      961 gataattcgt taagtgttaa tagtatcagc tctaatggta ctattcgttc caattcacaa
     1021 ttagacaaca gaacagttga atctacagta acatctacta atgaaaataa gagttataag
     1081 gaagatgtta taagtgacag aattatcaaa aaagaatttg aagatactgc tttaagtgta
     1141 aaagattatg gtgcggtagg tgatgggatt catgatgatc gacaagcaat tcaagatgca
     1201 atagatgctg cagctcaagg gctaggtgga ggaaatgtat attttcctga aggaacttat
     1261 ttagtaaaag aaattgtttt tttaaaaagt catacacact tagaattgaa tgagaaagct
     1321 acaattctaa atggtataaa tattaagaat cacccttcca ttgtttttat gacaggttta
     1381 tttacggatg atggtgcgca agtagaatgg ggcccaacag aagatattag ttattctggt
     1441 ggtacgattg atatgaacgg tgctttgaat gaagaaggaa ctaaagcaaa aaatctacca
     1501 cttataaatt cttcaggtgc atttgctatt gggaattcaa ataacgtaac tataaaaaat
     1561 gtaacattca aggatagtta tcaagggcat gctattcaaa ttgcaggttc gaaaaatgta
     1621 ttagttgata attctcgttt tcttgggcaa gccttaccca aaacgatgaa ggatgggcaa
     1681 atcataagta aggagagcat tcagattgaa ccattaacta gaaaaggttt tccttatgcc
     1741 ttgaatgatg atgggaaaaa atctgaaaat gtgactattc aaaattccta ttttggcaaa
     1801 agtgataaat ctggggaatt agtaacagca attggcacac actatcaaac attgtcgaca
     1861 cagaacccct ctaatattaa aattttaaat aatcattttg ataacatgat gtattcaggt
     1921 gtacgtttta caggattcac tgatgtatta atcaaaggaa atcgctttga taagaaagtt
     1981 aaaggagaga gtgtacatta tcgagaaagc ggagcagctt tagtaaatgc ttatagctat
     2041 aaaaacacta aagacctatt agatttaaat aaacaggtgg ttatcgccga aaatatattt
     2101 aatattgccg atcctaaaac aaaagcgata cgagttgcaa aagatagtgc agaatattta
     2161 ggaaaagtat cagatattac tgtaacaaaa aatgtagtta ataataattc taaggaaaca
     2221 gaacaaccaa atattgaatt attacgagtt agtgataatt tagtagtctc agagaatagt
     2281 atattcggtg gtaaagaagg aattgttatt gaggattcaa agggtaaaat aaccgtttta
     2341 aataaccaat tttataattt atccggtaag tatatatcat tcatcaaatc taatgcaaat
     2401 gggaaagaac ctgttatacg tgatagcgat ggtaatttca atattgtaac ggagaatggg
     2461 ctttacaaaa ttgtaacaaa taatttaagt gataaaaacg aaaaagaaaa aaacaaagag
     2521 gaaaaacaat ctaattcaaa taatgtaatt gatagtaacc agaagaacgg agagtttaac
     2581 tcaagtaaag ataatagaca aatgaatgac aagatcgaca ataaacaaga taataagaca
     2641 gaagaagtaa actataaaat agttggagat ggcagagaaa ctgaaaatca tattaataaa
     2701 tctaaagaaa tagtagatgt aaaacaaaaa ttaccaaaga ctggttcgaa caagattatg
     2761 gaactattct taacagtgac aggaattggt ttacttttga cactaaaagg gttgaagtat
     2821 tatggtaaag ataaataaaa tttgttcgat acaaggaagt tccgtagaaa atgaggatat
     2881 tgtaggttca caaaatcagt atttttggat tattggtgga gctacagatt tatataattc
     2941 taaagaggag ataggttatt cagtctcaga agttgtacat attttatcag aaagtttgtc
     3001 ggttaattgt aaagaatcaa aaactcttaa acaaatattt gaaactgctt tactcgaggt
     3061 taaagatgaa atcggtttaa actcatatga attaacagag tatagtaa
//
LOCUS       DAVHEX010000102         2724 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVHEX010000102 DAVHEX010000000
VERSION     DAVHEX010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.103"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(<1..319)
                     /locus_tag="VVL45_002086"
     CDS             complement(<1..319)
                     /locus_tag="VVL45_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188201.1"
                     /translation="MSEVDFNEAVNYEFTSDTCQLANSIYQSLFKFFDKKNFSGDLIF
                     TWKSPSLVKEGDYIGRRDSQVDNLRVIGNIFPNYLTNRKYSLNMNRNGCMGDFPHDFF
                     DIYL"
     gene            complement(377..1090)
                     /locus_tag="VVL45_002087"
     CDS             complement(377..1090)
                     /locus_tag="VVL45_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEU4188202.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFAHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYNLYAATNGITAIQTGRLAQSGLAPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            complement(1550..1924)
                     /locus_tag="VVL45_002088"
     CDS             complement(1550..1924)
                     /locus_tag="VVL45_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002960428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEU4188203.1"
                     /translation="MESIGLVIVSHSKHIAEGVVELISKVAKDVPITYVRGTEGGGIG
                     TSFEQVDRVVSENPADTLLAFFDLGSAKMNLKMVTDFSDKSIIINRVPIVEGAYNAAA
                     LLQAGAELSVIQTQLAELEINK"
     gene            complement(2346..>2724)
                     /locus_tag="VVL45_002089"
     CDS             complement(2346..>2724)
                     /locus_tag="VVL45_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEU4188204.1"
                     /translation="RNKNGQADTNQTEKPSEEKPQTEKPEEETPREEKPQSEKPESPK
                     PTEEPEESPEESEEPQVETEKVEEKLREAEDLLGKIQDPIIKSNAKETLTGLKNNLLF
                     GTQDNNTIMAEAEKLLALLKESK"
BASE COUNT          829 a          533 c          434 g          928 t
ORIGIN      
        1 ctaggtatat atcaaaaaag tcatgaggaa aatctcccat acagccatta cgattcatat
       61 tgaggctata ttttcgatta gtaagataat tcggaaatat atttcctatt actctaagat
      121 tatctacttg tgaatccctt ctcccaatat aatccccttc tttgactaat gatggagatt
      181 tccaagtaaa aattaaatcg ccagagaaat ttttcttatc aaaaaactta aaaagagatt
      241 gataaatact atttgctaat tgacaagtgt cagatgtaaa ttcataatta acagcttcat
      301 taaaatcaac ttcagacatt tcaatgtact cttcaatagt aatattagct tttttgtagt
      361 cattagctcg tctcctttaa aatgtgatct tttcaagaat atttttatct aaacaatcca
      421 gcaagtcttg gtaagaatag acttcgtaag tcggctgggc ttgtgtgtga ttttcgaggt
      481 gatgaggatt ataccagata gtgtcaatcc ccgcattatt gccaccttga atgtcggcgg
      541 ttagagaatc tccaatcatc agcgtctttt ctttactaaa tccagcaatt tgctggccaa
      601 tcttttcata aaaaagagca tccggctttt gagtttgcaa ctgttctgag ataaagactt
      661 gattgaaata aggtgctaga ccagattgag ccaaacgtcc tgtctgaatg gcagtaatgc
      721 catttgtcgc agcatacaag ttataatcac gctcaatgag gctgtccaag agatcatgag
      781 cgcccgatag tgtttgtccc tgctgggcga ggtaaaattg gtaacgctgg gcaagaaaac
      841 taccgtcttt ttcctgtcca aaatgagcaa ataaacgaga aaagcgcgtg ttaaccagct
      901 cttgtttact gattttcttc agctccaagt ctttccagag agccttgttc ataggaacgt
      961 aataatcttt ataagcctga atatccgcaa ctccttcttc ttttagaagt tgggtcaaag
     1021 ccacatcctc agcagcatca aaatcaagaa gagtgtggtc gaggtcgaag agtagaaatt
     1081 tgtagaacaa tttgaggttt tcctttctga aaattcatta agaacattat atcataaagc
     1141 acctcataca attaactaat ttaatcactt aaaaaaattc gaacactttc tatacaactg
     1201 acagctcaaa tctttcagaa tagaacaata ctaactatcg aacaccccgt cttcataaat
     1261 atatatatgt aattctagac ctagaattcc tataaactaa atgctttcat actcttccaa
     1321 gtaattgatt gccttaaatt ttaatttttg aaggtttcta aagctagaat agccccatca
     1381 caatcagttt tgattgattc acaatttaga aacactatag tttcactcct gttaaaataa
     1441 aaaggaactg cataaagcaa tccctttctg attttgaaat catttactta acattttata
     1501 gttgagataa tcaatagctt atctataaaa agagttatag taaaattcct tatttattga
     1561 tttcaagctc cgctaactgt gtttgaataa ctgacagttc tgcaccagcc tgaagaagag
     1621 cagctgcatt ataggcacct tctacaattg gaaccctgtt gatgatgata cttttatcac
     1681 tgaaatcagt caccattttt aagttcattt tagcagaacc taggtcaaaa aaggcaagta
     1741 aagtatctgc tggattttcg gaaacaaccc tatctacttg ttcaaaactc gttccaattc
     1801 ctccgccctc ggttcctctt acataagtaa tcggaacatc tttagctact ttactaatca
     1861 gttcaacaac accttctgca atgtgtttgg aatgtgaaac gataacaaga ccaatacttt
     1921 ccatcaaacc accccagttt ctaaaatagc agtaaagagt aatcctgatg agaatgatcc
     1981 aggatcaata tgtccaagaa accacatgct cctaagacaa gagctaacag actggccatc
     2041 aataatagta ttgttctttt tttcatcatt actccttaac tagtgtttaa ctgattaatt
     2101 agccagtaaa tagtttatct ttatttacac tatctgttaa gatatagtaa aatgaaataa
     2161 gaacaggaca gtcaaatcga tttctaacaa tgttttagaa gtagaggtat actattctag
     2221 tttcaatcta ctatattttg cacattttca tgaaaaaaat gagaactaga actcacattc
     2281 tgctctcatt tttcgttttc ccgttctcct atcctgtttt taggagttag aaaatgctgc
     2341 tacctttact tactctcctt taataaagcc aatagttttt cagcttctgc cataatagta
     2401 ttgttgtcct gggtgccaaa tagtaaatta ttttttaatc ctgtgagagt ctctttggca
     2461 ttggacttga taattggatc ctggattttt ccaagtaaat cttcagcctc tctcagtttt
     2521 tcttcaacct tttcagtctc gacctgaggt tcttctgatt cctctggtga ttcttctggt
     2581 tcctctgttg gttttggaga ctctggtttc tcgctttgcg gtttctcttc tcgaggggtt
     2641 tcttcctcag gtttttctgt ctgaggtttc tcctcgcttg gtttttccgt ttgattggta
     2701 tcagcttgac catttttgtt tctt
//
LOCUS       DAVHEX010000103         2627 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVHEX010000103 DAVHEX010000000
VERSION     DAVHEX010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.104"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..574
                     /locus_tag="VVL45_002090"
     CDS             <1..574
                     /locus_tag="VVL45_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU4188205.1"
                     /translation="KIPSRLYKIDTGKVLFDGVNINQIDKKILSQNLGVVPQDSFLLN
                     RSILDNITLKHEVTSQKIEEVCKAVQIYDEIMAMPMKFNTIISEMGSNISGGQRQRIA
                     LARALINNPSIVILDEATSALDTINEERITKYIQSQGCTQIIVAHRLSTIKDADVIFV
                     MKGGKIVESGNHKYLMDLGGEYYSLYTKRK"
     gene            587..757
                     /locus_tag="VVL45_002091"
     CDS             587..757
                     /locus_tag="VVL45_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188206.1"
                     /translation="MKKENEYVILTTASLGVMIGIVFAIFLDFPVEYGISLGLLNGIV
                     LGSLIVYKNNKN"
     gene            complement(1263..2609)
                     /gene="gdhA"
                     /locus_tag="VVL45_002092"
     CDS             complement(1263..2609)
                     /gene="gdhA"
                     /locus_tag="VVL45_002092"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEU4188207.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
BASE COUNT          846 a          453 c          508 g          820 t
ORIGIN      
        1 aaaaatacca tcaagattat ataagattga tacaggaaaa gttttatttg atggtgtaaa
       61 tattaatcaa atagataaaa aaatattaag tcaaaattta ggagtagttc cacaggattc
      121 atttttattg aaccgaagta ttcttgataa tataacttta aagcacgaag ttacttcaca
      181 aaagatagag gaagtttgta aagcagttca aatctatgat gaaatcatgg ctatgccgat
      241 gaaatttaat actatcatct cagagatggg gtcaaatatt tcaggtgggc aaaggcaacg
      301 gatagcactg gcacgtgcat taataaataa tcctagtatt gtaattttag atgaagcaac
      361 tagtgcatta gacactatta atgaggaaag aataacaaag tatatacaaa gtcagggctg
      421 tactcaaata attgtagctc atagattgtc aacgattaag gatgcggatg ttatttttgt
      481 aatgaaaggt ggtaagattg ttgagtcagg aaatcataag tacttaatgg atcttggtgg
      541 agagtactac agcttatata caaaaaggaa atgaggtgta aagaaaatga agaaagaaaa
      601 tgaatatgta attttaacaa cagcctcact aggggtgatg attggaatag tgtttgcaat
      661 ttttttagat tttccagttg aatatggtat ttctttaggc ttgttgaatg gaatagtatt
      721 gggttcgctg attgtttaca aaaacaataa gaattaagca taattttttg ctgtaaacta
      781 aggagtagag atggctatag ttgaaattat aaatctaaca aaaagcttta aagatattga
      841 agttattcat aacacttaaa taatagagca actacagtag tagcttaaaa acatgattaa
      901 atcgctattc ttaggagtag cggtttttct ttttgtttaa tactctttga aaatctcttc
      961 aaaccacgtc agctttgctt taccgtactc aagtacagcc tgcggctcgc ttcctagttt
     1021 gctctttgat tttcattgag tataaaaagg gtcaagtaag tatagtaaat tgaaataaga
     1081 tatgaacaaa tcgattagaa aagtcaaatt aatttctaga aatatgttag aaattggttt
     1141 gaattccgca atcaatttgt tcagttttta tttcatttca ttttatttaa ttagattttc
     1201 caatttttta attcaagcta aaaatcccca atcgtagtga ttgaggattg agtaaataaa
     1261 tcttaaacaa taccttgtgc aatcatggca tttgctacat tttcaaaggc agcaatgtta
     1321 gctcctgcaa ggtagtcttt atcaagaccg tatgtttctg aagtcgtttt agctgtgttg
     1381 aagatgtttg tcatgatgtc tttgagacgg ccatcaactt cttcacgagt ccatgagagg
     1441 cgaagactgt tttggctcat ttcaagagct gaaacggcta caccaccagc gttggcagct
     1501 tttgcaggtc cgtagaagat accattttct ttgtaaactt tgatggcatc aaggtcgctc
     1561 ggcatgttgg caccttcaga tacacagata acgccttgag caaccaaacg tttagctgct
     1621 tcaccgttga tttcgttttg agtggcacat ggaagagcaa tgtcatagtt tccagcgtaa
     1681 gtccatacag taccttcgtg gtaggttgca gttgcttttt cagctgcata ctcagtcaaa
     1741 cgagcacgac gtttttcttt aacatcaacc aaaagatcga agtcgatacc attttcatcg
     1801 atgacataac catttgagtc agaaacagaa ataacagttg caccgagttc agttgctttt
     1861 tgaagagcat attgagcaac gttaccagaa cctgaaataa cgactttctt accagcaaag
     1921 ctgttaccgt tagctttgag catttcttca gtatagtaaa ccaaaccgta accagttgct
     1981 tctggacgaa tcaagctacc accaaatcca agaggtttac cagtcaagac accagcatca
     2041 aattggttaa gacgtttgta ttgaccgtaa aggtaaccaa tttcacgtcc accaacaccg
     2101 atatcaccag caggtacgtc aagtgatggt ccgatgtgtt tttgcaattc agtcatgaag
     2161 ctttggcaga agcgcatcac ttcagcatct gttttacctt taggatcgaa gtctgatcca
     2221 cctttacctc caccgatagg aagtccagtc aagacatttt taaagatttg ttcaaatccg
     2281 aggaatttca agatcccttg gtttacagtt gggtggaaac gaagtccacc tttgtatggt
     2341 ccaacagctg agttgaattg aacacggtaa ccacggttta cttgaatttt tccatcacgg
     2401 tcaacccaag gaacacggaa agaaaccacg cgctcaggct cagtaatacg tgccaagata
     2461 ttttcttcga tatactcagg gtgtttttca aatacaggtt ctaaagtgtt gaaaaattct
     2521 tcaacagctt ggaggaattc agcctcgtgc ccgttacgag ctttcacagt ttcaaacacg
     2581 ctttggatat attctttagc agatgtcata tcgttctcct tttattg
//
LOCUS       DAVHEX010000104         2442 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVHEX010000104 DAVHEX010000000
VERSION     DAVHEX010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.105"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            34..1059
                     /locus_tag="VVL45_002093"
     CDS             34..1059
                     /locus_tag="VVL45_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU4188208.1"
                     /translation="MKNKFFLIAILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDH
                     AFGQTILDKKPERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKE
                     LNGKANLFDDLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQT
                     LWRDMIKIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAAD
                     TSKFWIYTSKDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITY
                     GDDKTLEALQKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLG
                     NACKNAK"
     gene            1049..2056
                     /locus_tag="VVL45_002094"
     CDS             1049..2056
                     /locus_tag="VVL45_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU4188209.1"
                     /translation="MRNKKQINLGIIFIICLGLLITIFLSLKLGTKEINIRDFLAAFG
                     MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGAS
                     LSVVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSM
                     ALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLN
                     ALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISK
                     DIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVN
                     N"
     gene            2056..>2442
                     /locus_tag="VVL45_002095"
     CDS             2056..>2442
                     /locus_tag="VVL45_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEU4188210.1"
                     /translation="MQNLIIGIQKRKNRITLFSSLFLLIIISLSFFILLIGDESYSFS
                     TLIKVLNSETVPGASFSIMEIRLPKLLAGIIAGWSFGLAGFIFQTMLRNPLASPDIIG
                     VTSSSSIAAVFCILVLKTNSLTTGIIS"
BASE COUNT          851 a          344 c          424 g          823 t
ORIGIN      
        1 acaatagact tgaaatatgt ttaaggagtt tttatgaaaa acaaattttt tctaatagct
       61 attttagcta tgtgtatagt ttttagcgct tgttcttcta attctgttaa aaatgaagaa
      121 aatacttcta aagagcatgc gcctgataaa atagttttag atcatgcttt cggtcaaact
      181 atattagata aaaaacctga aagagttgca actattgctt ggggaaatca tgatgtagca
      241 ttagctttag gaatagttcc tgttggattt tcaaaagcaa attacggtgt aagtgctgat
      301 aaaggagttt taccatggac agaagaaaaa atcaaagaac taaatggtaa agctaaccta
      361 tttgacgatt tggatggact taactttgaa gcaatatcaa attctaaacc agatgttatc
      421 ttagcaggtt attctggtat aactaaagaa gattatgaca ctctatcaaa aattgctcct
      481 gtagcagcat acaaatctaa accttggcaa actttatgga gagatatgat taaaattgat
      541 tcaaaagcct taggtatgga aaaagaaggt gatgagttaa tcaaaaatac tgaagctcgt
      601 atatccaaag aattagaaaa acatccagaa atcaaaggaa aaatcaaagg aaaaaaagta
      661 ttatttacta tgattaatgc tgcagataca tcaaaattct ggatttatac tagcaaagat
      721 ccaagagcaa attatttaac agatttaggt ctagttttcc ctgaatcatt aaaagaattt
      781 gagagtgaag atagttttgc aaaggaaatt tctgcagaag aagcaaataa gataaatgat
      841 gctgatgtaa tcataactta tggtgatgat aaaactcttg aagctttaca aaaagatcct
      901 cttttaggta aaataaatgc aattaaaaat ggtgccgttg ctgtaattcc agataataca
      961 ccgttagcag cctcatgcac tccaacacca ctttcaataa actatactat tgaagaatac
     1021 ctaaatcttt taggaaatgc atgcaaaaat gcgaaataaa aaacaaataa acctaggcat
     1081 aatttttata atctgcctag gtcttcttat tacaatattt ttgtcattaa agcttggaac
     1141 aaaagaaatt aatatcagag attttttagc agcttttgga atgggtaata caaatgatga
     1201 ttttattaaa tcaattatat ataaaagaat acctagaact atttttgcaa ttttagcagg
     1261 ttctagtctt gccataagcg gtgtattgat gcaatcagtt actagaaacc caatagctga
     1321 tccaggtata ctcggtataa acacaggagc aagtcttagt gtagtaattg gtctttcttt
     1381 tttaggaatt tcatcaagca taagccatat aagttttgca atcattggtg gcttagtaag
     1441 tgcaattttt gtatacgcga ttgctgtaag cggaaaagca ggccttaccc ctataaaact
     1501 tgccttatca ggaacttgtg ttagtatggc tttaagcagt tttgtaagtt ttttaatttt
     1561 accgaataat aacgtcttag acaaatttag attttggcaa ataggtagcc ttggagcagc
     1621 tacattatct tctatatcta cactactacc ttttataatt ttaggtcact tgatagctat
     1681 atttatttca tcagatttaa acgctttagc tatgggtgat gaaatggctg ttggtcttgg
     1741 agttaatgtt aataggataa gatcacttgc aataattgca agtgtgcttt tatgttcaag
     1801 tattactgca attggtggac ctattggctt cgtaggtctt atagttcctc acttttgtgg
     1861 cttatttata agcaaagata tacgcacaat gaccatttct tcatctttta taggtgcaga
     1921 gctcttgctt atatgtgata taatcggccg tatgttaggt aaaccaggtg aaattgaagt
     1981 agggataatt actgcaataa tcgggggtcc agtacttatt tatgtaacta tgaaaaatag
     2041 aggggttaat aactaatgca aaatttaatt ataggtattc aaaaaagaaa aaatagaata
     2101 acactatttt cctcactatt tttattaata ataatcagtc tatcattttt cattttactt
     2161 atcggagatg aaagttattc tttttcaact ttgattaaag tcttaaatag tgaaactgtt
     2221 cctggagcta gtttttcgat tatggaaatt agattaccaa aattattagc aggaattata
     2281 gctggctggt cttttggatt ggcaggattt atctttcaaa ctatgttaag aaatcctctt
     2341 gcaagtcctg atataatcgg tgtcacaagt tcttcatcta ttgcagcggt cttttgcata
     2401 ttggtattaa aaacaaatag tttaactact ggaattattt ca
//
LOCUS       DAVHEX010000105         2413 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVHEX010000105 DAVHEX010000000
VERSION     DAVHEX010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.106"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            96..260
                     /locus_tag="VVL45_002096"
     CDS             96..260
                     /locus_tag="VVL45_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU4188211.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAEMALLPSIVGAAIVI
                     TVVSFFTGRK"
     gene            344..910
                     /gene="amaP"
                     /locus_tag="VVL45_002097"
     CDS             344..910
                     /gene="amaP"
                     /locus_tag="VVL45_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEU4188212.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            922..1092
                     /locus_tag="VVL45_002098"
     CDS             922..1092
                     /locus_tag="VVL45_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEU4188213.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            1131..1739
                     /locus_tag="VVL45_002099"
     CDS             1131..1739
                     /locus_tag="VVL45_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU4188214.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            1770..1973
                     /locus_tag="VVL45_002100"
     CDS             1770..1973
                     /locus_tag="VVL45_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU4188215.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(2084..2356)
                     /locus_tag="VVL45_002101"
                     /pseudo
     CDS             complement(2084..2356)
                     /locus_tag="VVL45_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547411.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          833 a          369 c          466 g          745 t
ORIGIN      
        1 taaaataaaa aaggagggta ggcagtgttg tgatcaatta ttgctggagg tcttattggt
       61 ctcttggcag gtaaaatcac taaaaaagta gttctatggg aatcatcgca aatgtattcg
      121 ctggtttagt cggggcatat gcaggacaat ctcttttagg tagttggggt ccatcaatcg
      181 ctgaaatggc tttgctccca tctattgtag gtgcagcgat tgtgattact gtagtgtcat
      241 tctttacagg tagaaagtaa acttttcgcc agtaaagtta gcaaactatt tttaaatcaa
      301 tgacgggaaa aatagtttaa atgttaaatc gaaaggattg tatatgtcaa aagcaaagaa
      361 aatatgtttc attattttct gtattttaat cttgacaatt ttccttcctg ttttgataga
      421 ttatcatcaa gttagtgatc taggtattca tctacttagc tggagacaga actccgtagt
      481 tgaattctat cttgctagat atgtcttttg ggggacagtg gttctatcaa ctttagtttt
      541 attatccatt ttagttgtga tgttttatcc taaacgttac ttggaaatcc aacttgaaac
      601 taaaaacgat acattaaaat taaagaattc ggcaatcgaa ggttttgtta gaagtttggt
      661 gagtgatcat agattgatca agaacccaac tgttcatgta aatttacgaa aaaataaatg
      721 tttcgttcat gtagaaggta aaattcttcc ttcagacaac atcgctgaca gatgccaaat
      781 aattcaaaat gaaataacta atggattgaa gcagtttttt ggtattgagc gtcaagtaaa
      841 acttgaagtt gcagtaaaaa attaccaacc aaaacctcaa aacaaaaaga ctgttagtcg
      901 tgtgaagtaa ggaagtaaaa aatggaatgg cttaaacaat atcgatatcc aattatcgct
      961 ggtctcatag gcgtatttct ggcttgtttg attgtctcct ttggcttctt caaaacaata
     1021 tttgtattga ttttaggagc actgggagtt gcagctggat tatatatcga aaaaaactat
     1081 atagataaat aaaaaaataa aaattactaa tttaattaaa ggagtttcat atgtcaaacg
     1141 aaaaaaacac aaacactaac gtagaaaaga aagatgctac tgttgtagct cacgaaatca
     1201 aaggggaact tacttacgaa gataaagtta tccaaaaaat cattggtctt tcactagaaa
     1261 acgtttcagg tcttttggga atcgatggtg gtttcttctc aaatcttaaa gaaaaaatcg
     1321 ttaacagcga tgacgtaaca agtggtgtta acgtagaagt tggtaaaaca caagttgcag
     1381 ttgacttaaa cgttattgtt gagtaccaaa aaaatgttcc agctttatat tcagaaatca
     1441 gagaaatcgt atcttcagaa gttgctaaaa tgactgactt ggaaattgtt gaaatcaacg
     1501 taaacgttgt cgacatcaaa actaaagaac agcatgaagc agactcagta agccttcaag
     1561 atcgcgtatc tgacgttgct gaatcaacag gagaattcac ttcagaacaa ttcgaaaaag
     1621 ctaaatctgg tcttggatct ggtttctcaa ctgttcaaga aaaagttagc gaaggtgtag
     1681 aagctgttaa aggtgcagca aatggtgtag tatctcacga aaacactcgt gtaaactaag
     1741 ataaaataaa tataacagga gaaattatca tgtcagtaga agaaaaatta aatcaagcta
     1801 aaggttctat taaagaaggt gttgggaaag ccatcggtga tgaaaaaatg gaaaaagaag
     1861 gagcagctga aaaagttgtt tctaaagtaa aagaagttgc cgaagacgct aaagacgctg
     1921 tagaaggtgc tgtagaaggt gttaaaaaca tgttgagtgg cgacgataaa taaggttaaa
     1981 agttacttta tcttttttgg taacattagt caaaagagtc tgagtcaaga tgattctcag
     2041 aaaacaaaaa gctagagatt cccaattgcg gaactctagc tttttaattt tgcctctttc
     2101 tcttattata tttcagcagg ttgttggcca tgagtatgaa tcccatgtca attctcactt
     2161 gacgcttacc tctcagatga catctcttat aacccaaaca aacctttatc tgcccaaaga
     2221 cagatttcat atcaatctta cgtttagcga aaatttgtct acccttggaa gataaaagtg
     2281 cctgatattc tttagttttt aaacactggt aacgttcatt catatacagt cccttttgag
     2341 gggctgattc aggttcataa tcgcagtcaa cattgatttc aaggctgttt gctttctatc
     2401 tccccttttt cat
//
LOCUS       DAVHEX010000106         2227 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVHEX010000106 DAVHEX010000000
VERSION     DAVHEX010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.107"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(316..1791)
                     /locus_tag="VVL45_002102"
     CDS             complement(316..1791)
                     /locus_tag="VVL45_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU4188216.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTDKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSILLIGEQMNSEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEEVIELSEYARREINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDDSVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
BASE COUNT          661 a          496 c          413 g          657 t
ORIGIN      
        1 ataatcggca agtagaacta aaaatactga ctagacatat tcatcgtctc aagaaaaaac
       61 agtctgattg gaaagtacaa tacactcgtt gtcttgatat catctttcct gagttggata
      121 aaatcgttgg aaagcattca gaatatacct accaactctt gacacgctac cctaatcctc
      181 agaaaaggat tgaggcagga tttgataagc tgatagaaat taagcgattg accgcttcta
      241 attcaagggc tttttgccgt gctctaaaat catcgccaaa tcatcaaatt gaaataagaa
      301 atagtttaga taaaactatt gacttttctt atagtttgtc tttctcttaa taacgttgat
      361 atgattgacc tctggatctt ccgtcccttg gagggagcaa ccacgttcct tggcgaattg
      421 gatatagtcg acaatttctc gtgtaatgcg ttcaccagga gccaagatag gaatacctgg
      481 agggtaacac ataacaaatt ctccacagac ctgtccaaca gaatcatcca aagttaaact
      541 ttttctttct gaatagaagg cttcttgcgg agacagcact aactcgggct gaatatattc
      601 tcctgctatc aaatcttttc catctcttga atagagtctc ttaatatcag ccagagcacc
      661 aaccaagcgc tcgatgtctt ggatgcggtc gccgatggaa atataggcca agatattgcc
      721 gatatcacca aactcgatct gaatgtcgta ttcgtctcgc aagaggtcat aaacctcgat
      781 acctgttaag ccaataccct gagtgtaaac tgacaactta gttacgtcaa aatcgcaaac
      841 cgaaacaccg tctattaact cttttgagta ggcatagtag ccaccgatag cattgatttc
      901 acggcgggca tactcagata gctcaatgac ttcctcaaac gactctttac cacgaagggc
      961 caagttgcga cgtgaaatat ccaaactagc catcaacaag taagaggcag atgtagactg
     1021 ggtcaggtta attatctgac gaacgtattc agaattcatc tgctccccga taagtaaaat
     1081 ggagctttgg gtcaaactcc caccagactt atgcatggaa actgctgcca tatcagcccc
     1141 tgcatccata gcagaaattg gaagtttatc agtgaaatgc aaatgcgctc cgtgggcttc
     1201 atctactaaa accatcatgc cagcttcatg agccatttct gtcaacccct ttaggtctga
     1261 acagatgccg tagtaagtag gattgttgat taggatagcc ttagcatctg gatggtcctt
     1321 tatggcctgt gctactcggt cattttcaag acctaaagcg ataccaatct taggatctac
     1381 actcatctcg atatagatgg gaatggcacc acatagaacc aacgcattga tagcagattt
     1441 atggacattt cgtggcagaa taatcttatc tcctgccttg caggttgcca gaatcatagt
     1501 ctgcaccgat gaagttgttc caccaatcat tagaaaggca tggctagctc caaaagcatc
     1561 tgcagccagc tcctctgcat cacgaataat cgaaatagga tggcctaaat tatccaaggg
     1621 tttcatcgaa ttgacatcaa tgcctacaca tttttctcct aagagttcga caagttctgg
     1681 atttccccgt ccacgcttgt gacctggaac atcaaaggga acaatccttt tcttgcgcaa
     1741 cttcaccaag gcctcataaa ttggggcttg gttttgatct aactctttca aaacatactc
     1801 ctttcaatat ttttaggacc ttatgaaaac taaggaacta aaggttgact catgaaaaaa
     1861 gagcaggttt tcacctgctc tgcaatcttc tgacgtgttt attttggttt ctgttgagta
     1921 tgtctgttcc cgatgtttcc taactctcta gtagcaaata ttacgtactt tattgatcgt
     1981 ttcacaagat gacgtgtgct ttcaccacac taaaaattta tgaattagga caagtgtcct
     2041 aacatcaact tataaaagta ggcttttaac cctatcaata atgtggcttt tcaaccacgc
     2101 ttcggcattc aaccctgcct gtccgtaggc attttttact cgggtttata tttgctagaa
     2161 aacatcatct cattttctaa gctttattac tatatcatgt ttttaagaac ttgtaaagta
     2221 ttttttg
//
LOCUS       DAVHEX010000107         2016 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVHEX010000107 DAVHEX010000000
VERSION     DAVHEX010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.108"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            1..460
                     /locus_tag="VVL45_002103"
                     /pseudo
     CDS             1..460
                     /locus_tag="VVL45_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
     gene            360..806
                     /locus_tag="VVL45_002104"
     CDS             360..806
                     /locus_tag="VVL45_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU4188217.1"
                     /translation="MRKHGLISKSTSKRYYQVAIPFMRLFCLVLVSIDYINCLSETTD
                     KNWHKSDRIFVTNTGKTVHSSILSKSLQRANERLKKPIPKHLSPHIFRHTTISILSEN
                     KIPLKTITDRVGHPDSEVTTSIYTHVTKNMKDEAINVLDKVMKKIF"
     gene            complement(1126..1302)
                     /locus_tag="VVL45_002105"
     CDS             complement(1126..1302)
                     /locus_tag="VVL45_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188218.1"
                     /translation="MDFLNHSFDTKKVINTKINAVNSKNNVGKNFIDVYREMKEVPNN
                     KIHQSKVNVALIKK"
     gene            complement(1305..>2016)
                     /locus_tag="VVL45_002106"
     CDS             complement(1305..>2016)
                     /locus_tag="VVL45_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188219.1"
                     /translation="EAESNFEFFEAELELIKSIVDDVFKSFPHTDDEVEVIAKSLYKA
                     IFIGYDSTNITGLVIAGYGTDEIFPSIRQIELYGIFSKRLIWKVINESVINHHKTCHI
                     IPFAQSEMVETIMNGIDPNLNVYIAEQVSSVMEKNGLGDEIENIFENISSIQQKYYIN
                     PIIDLIGMQPLNEMASTAKTFIELTSFKRKIVNTLETVGGPVDVLAISKGEGPIWIDR
                     KYYFDIDKNLDYRMRKES"
BASE COUNT          673 a          367 c          294 g          682 t
ORIGIN      
        1 aacacctgtt tagattgacg aaacaggatt cgatacttat ttttatcgag aatatggtcg
       61 ctcattaaaa ggtcagttaa taagaggcaa agtatctgga agaagatatc agaggatttc
      121 tttggttgca ggtctaacaa atggtgaatt aatcgctcca atgacttacg aagagacgat
      181 gacgagcgac ttttttgaag cttggtttca gaattttctc ttaccaacat taaccacacc
      241 atcggttatt attatggata atgcaagatt ccatagaatg gggaagctag aactcttgtg
      301 tgaagagttt gggtataaac ttttacctct tcctccctac tcacctgagt acaatcctat
      361 tgagaaaaca tgggctcata tcaaaaagca cctcaaaaag gtattaccaa gttgcaatac
      421 cttttatgag gcttttttgt cttgttcttg tttcaattga ctatataaat tgtctaagcg
      481 aaacaaccga taagaattgg cacaaaagcg accgtatttt tgttaccaat acaggaaaaa
      541 cagttcatag ttctatcttg agcaagtctc tccagcgagc aaacgaacgc cttaaaaaac
      601 caattcccaa acatctgtcc cctcacatct tcagacacac cactattagc atcttatcag
      661 aaaataaaat tcctttaaaa acaatcacgg acagggttgg tcatcccgac tctgaagtca
      721 ctacttccat ctacacccac gtcacaaaga acatgaaaga tgaagcaatc aatgtactgg
      781 ataaagttat gaaaaagatt ttttaaaaag ttttgtccct tttttgccct ctaaatacaa
      841 aaatagccct tcggataaaa tccgaggggc tagaaacgtt gttaaatcaa cggccgaact
      901 tttgaatttc atggttcggg ataaaatagt tcactgaact attttatttt ttaaggttat
      961 cataatatca aatagttcaa ttaaatacgc taaattacta atatactttt tacctttttc
     1021 attctaaaat gtaaagtaca aacaattaca atatactaga gggggagtaa aaaaggtatt
     1081 aaatcgatga gttcagcagg caagaaaata gcacctttac gggtgctatt ttttaattaa
     1141 cgccacgtta acttttgatt gatgaatttt attgtttggc acttctttca tttcacggta
     1201 aacatcgatg aaattctttc caacattatt tttggagtta actgcattta tttttgtatt
     1261 aataactttt ttagtatcga aagaatggtt taagaaatcc ataactaact ctcctttctc
     1321 atcctgtaat caagattttt atcaatgtca aaatagtatt ttctatcaat ccaaattggt
     1381 ccttctcctt tagaaatagc aagtacatct accggacctc ctactgtttc aagagtgttg
     1441 acaatttttc tcttaaatga agttaattca ataaatgttt tagctgtact cgccatttca
     1501 ttaagtggtt gcattccaat aaggtctatt ataggattta tataatattt ttgctgtata
     1561 gatgatatat tttcaaatat attctcaatt tcatcaccca atccattttt ctccataact
     1621 gatgatactt gctctgcgat atatacattt aagttaggat ctataccatt cataatcgtc
     1681 tcaaccatct ctgactgtgc aaaagggatt atatgacaag ttttatgatg atttatcaca
     1741 ctttcattaa taactttcca aattaatcgt ttagaaaaaa ttccatataa ttcaatttgt
     1801 cttatagatg gaaatatctc gtctgtacca taacctgcta taactaatcc agttatgttt
     1861 gttgagtcat atccaatgaa aatcgcttta tataaagatt tagcaataac ttcaacctca
     1921 tcatcagtat gaggaaagga tttaaaaaca tcgtctacaa tgctttttat taactctaac
     1981 tcagcttcaa aaaattcaaa attactttca gcttct
//
LOCUS       DAVHEX010000108         1978 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVHEX010000108 DAVHEX010000000
VERSION     DAVHEX010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.109"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            144..848
                     /locus_tag="VVL45_002107"
     CDS             144..848
                     /locus_tag="VVL45_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEU4188220.1"
                     /translation="MNKIFIYAGVRNHNSKTLEYTKRLSSIISSRNNVDISFRTPFNS
                     ELEISNSDSEELFKKGIDRQSNADDGGVIKKELLESDIIIISSPVYLQNVSVDTKNFI
                     ERIGGWSHLFRLAGKFVVTLDVAESNGSDNVSEYLRDIFSYMGGQILHQVSITNSLKD
                     IAEAQLMEATYKIEDVLEGKIKYKTTDYQERAYQTLKLILENYDSEHFEKMYWEKKRL
                     FEANSLEEWYYVENIK"
     gene            829..1758
                     /locus_tag="VVL45_002108"
     CDS             829..1758
                     /locus_tag="VVL45_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEU4188221.1"
                     /translation="MLKILNNSVIYLCLIPLLFLLLIIFPNDSLIYYFRLILISLISI
                     TLHELGHFLVGRCLSYKLEMLATPFFFYFRKKIYFKFPVLLAFGYCQMSNRNITNEKN
                     SDRNLVFYFFGGGGANLIVAILALLGFVPYASEFFILNIILFLVTVCLPIDGTDGNAI
                     REIVLYSKDSKTYQRFFANSLYNNPYITIDDFAKLTSKEKSFFSKFEKCLINLYFEVK
                     GEGSAFTIANHEVFDSNIQENIIHEYYKLLHKSSDWIIPQNLSLEEVVFTLSNYIYSK
                     NNKYLEKIKYLKKLVDFRQEEIIDFILNKEEVL"
     gene            1755..>1978
                     /locus_tag="VVL45_002109"
     CDS             1755..>1978
                     /locus_tag="VVL45_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEU4188222.1"
                     /translation="MKIPMIYQMENSECGLACCAMILNYFKYEISLNELREIYPSSRS
                     GYSLLSISKVLGDFNISSHAFKASVRDLKPL"
BASE COUNT          711 a          243 c          328 g          696 t
ORIGIN      
        1 actaaaatca aaattactcc aaaaatagta ttgattttgg tttaatacaa aacatatgta
       61 ataatgtgag tcaacaaaag tctgtttttg taccatacta tcttgaaaaa tctactaaat
      121 tcaaattgag aggatgaatt aaaatgaata aaatatttat atatgcagga gtgcgaaatc
      181 ataactctaa gactttagag tatactaaga gactttcaag tatcatttct agtagaaata
      241 atgttgatat atctttcagg acacctttca attcagagct agaaatatct aattctgatt
      301 cagaagagtt gtttaagaaa ggaattgata ggcaaagtaa tgctgatgat ggaggtgtta
      361 taaaaaaaga gttattagag tctgatatta taattatctc ttctcctgta tatttacaaa
      421 acgtatctgt ggacacaaaa aactttattg aaaggattgg ggggtggagc catctttttc
      481 gattagctgg aaagtttgtt gtaacattag atgttgctga gagtaacggc tctgataatg
      541 tgtcagaata tcttagagat atattttcat atatgggagg acagatatta caccaagttt
      601 caattaccaa ttcactcaaa gatattgcag aggctcagtt gatggaggca acttataaaa
      661 ttgaagatgt acttgaaggg aaaattaagt acaaaacaac ggattatcaa gaaagagctt
      721 accagacatt aaaattaatt ttagaaaatt atgattccga acattttgag aagatgtatt
      781 gggagaaaaa acgccttttt gaagctaata gtttagagga atggtattat gttgaaaata
      841 ttaaataact ctgtcatata tttatgtttg attccgttat tgttcctatt attgataatt
      901 tttccaaatg attcattaat ctattatttt cgattgattt taatttcgtt aatatcaatt
      961 acattacatg aactaggtca ttttttagtg ggaagatgtt tatcttataa actagagatg
     1021 cttgctactc cctttttctt ctatttcaga aaaaaaatat atttcaaatt tccagtgtta
     1081 ttagcattcg gttattgtca aatgagtaat agaaacatta ctaatgaaaa aaatagtgat
     1141 aggaatttgg tgttctactt ctttggggga ggtggagcta acctaattgt cgcaatatta
     1201 gcacttttgg gatttgtgcc gtacgcatca gaatttttta ttttgaatat aattcttttt
     1261 ctagttacgg tatgtttacc aattgatgga acagatggca atgctattag agagatagtt
     1321 ttgtattcaa aagattcaaa aacatatcag agattctttg cgaacagtct ctataataat
     1381 ccttatataa caatcgacga ttttgcaaaa ttaacctcta aagaaaaatc ttttttttca
     1441 aaatttgaaa aatgtttgat aaacctttac tttgaagtga aaggtgaagg gtcagcattt
     1501 acaattgcta atcatgaagt ttttgattca aatattcagg aaaatattat ccatgagtac
     1561 tataaattac tacataaatc ttctgattgg attattccac agaacttatc tttagaagaa
     1621 gttgttttta ccttgtcaaa ctatatttac tcgaaaaata ataaatattt agaaaagatt
     1681 aagtatctaa agaaattagt agattttaga caagaagaaa ttatcgactt tattttgaac
     1741 aaggaggaag tattgtgaaa attccaatga tatatcaaat ggaaaattca gaatgtggat
     1801 tagcatgctg tgccatgata ttgaactatt ttaaatatga gatttcttta aatgaactac
     1861 gtgaaatcta cccatcatcc agatctggat attctctcct atctataagt aaagttttag
     1921 gagattttaa tataagttct catgctttta aagcttcggt aagagattta aaaccgct
//
LOCUS       DAVHEX010000109         1976 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVHEX010000109 DAVHEX010000000
VERSION     DAVHEX010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.110"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(<1..153)
                     /locus_tag="VVL45_002110"
     CDS             complement(<1..153)
                     /locus_tag="VVL45_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU4188223.1"
                     /translation="MSSIHTKNSSLKSKSRFNEMFGDPLNNNKKFAVKTGQQCFKFSS
                     GKFLDKH"
     gene            complement(119..1084)
                     /locus_tag="VVL45_002111"
     CDS             complement(119..1084)
                     /locus_tag="VVL45_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU4188224.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRRIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLASTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKAQRLGIAGVEIRLRKLGDKLGIQKVHPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLAYAMVNQNNVKHSHQKFIS"
     gene            complement(1202..1615)
                     /locus_tag="VVL45_002112"
     CDS             complement(1202..1615)
                     /locus_tag="VVL45_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEU4188225.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSSSALNMIVNLPEQ
                     FVFGKSLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWVSEHSVREKVKK"
     gene            complement(1612..1848)
                     /locus_tag="VVL45_002113"
     CDS             complement(1612..1848)
                     /locus_tag="VVL45_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEU4188226.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
BASE COUNT          576 a          384 c          291 g          725 t
ORIGIN      
        1 atgtttatca agaaattttc cacttgaaaa tttaaaacac tgttgtcccg tttttactgc
       61 aaatttcttg ttattattta aaggatcccc aaacatctcg ttaaatcggg atttgctttt
      121 aagagatgaa tttttggtgt gaatgcttga cattattttg attgaccatg gcataggcca
      181 gggttgtgtc aatcttgcta tgacctagca gtttttggac ttgttcgata ggcatacctt
      241 tatcaattgc cttagtcgct aaagttcttc tgaacttatg tgggtgaacc ttttgtatgc
      301 cgagtttgtc tcctaacttt cttaagcgaa tctctacacc agcaattcca agtctctggg
      361 cttttccaac tagcgttaca aaaagagcag ggtgactatc ttttctgtcg ttaagataat
      421 ttcttaaatg aattttcgtc cgagcgtcaa aatatactgg tctctccttc tttcctttac
      481 caaagacaac acactctctg ttttcaaaat caatatctga acgattcaac tgtacaagct
      541 cccctacacg catacctgtc gatgctagta ggtctattat tgccaaatct ctcaaatttt
      601 cacagttatc acgcataatt tccaaatgtt catcagtata agtttccttc acattttgct
      661 cagtcttaat tttctttatc cgtcgaatgg gatttttaat gatatattcc tcttgctcaa
      721 gccaagcaaa aaaagaagac aatatacgcc taatattgtc taaatttgcc ttactacaat
      781 ccttttcaga ctggtaattt gctaaataac tccttaaatc atctgttgtg agttgtgtca
      841 cagactcttt aatagcatta aacaagtttt caatcgtcct ctggtaataa cgaattgttt
      901 ttgagctaca gccctctacc tgcttggcgg taataaatag ttgcaacagg ttattattcg
      961 aatacttctg ctgctttttt ccgtctacta gatgatgttc taaactctct ctcaacgcta
     1021 gtaattgttc attatttaaa agaggtaaca tttcttgcat gataattgta acttttgatt
     1081 ccataatttc tcactttctt attcatcaat aaaaagagac aatatcagtt tctgcattac
     1141 agaattctaa tattgtctgt tcatttattt tcttgtgctc attcaaatcc agcatactag
     1201 gttacttttt gaccttttct ctaacagaat gttcagaaac ccatttggag tagcttgtca
     1261 ttttttcatc agttaaagct tctactgcga tggttacaca catttttact gcttcttcta
     1321 ccgttacaga aaaacgatag ccgtttaatt gtaaaaattt gaccaaaacg aagaaagcag
     1381 ttcttttatt agcattagca aaaacatgct tctttatcaa ttggacaaat agtatcgttg
     1441 ctttatcaaa aattgttgga taaagagact tcccaaagac aaattgttct ggtaagttca
     1501 caatcatatt taaggcagaa gaactaactg tttgaatttt ctcatttgga gaataccgtt
     1561 gaattgctaa agcatttatt ttttcaattt gcttttctgt cagatagatt gtcatttttc
     1621 caccaaagct ttgaaaacat cgtcgtattc gtcaaatatt ttatctaaat ttaagtcaaa
     1681 ggcatcatca gatacataag aaacctgctg ccccaattct tcaggagtaa agataatctc
     1741 accacttggt aacaatttcg cttcgtattt aacaccactt ggaatattaa attcacttgg
     1801 aatcgtaatg gtgattgaat ttccttgttt tcttgttttt actaccatct gatttcctcc
     1861 tttgaagtat tataacaaat cattaccgta attacaatgg cagaatatca gccaaaatac
     1921 tcctgcatca gagatttctt caaagtttca agttcttcca gagatttttg gattgc
//
LOCUS       DAVHEX010000110         1957 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVHEX010000110 DAVHEX010000000
VERSION     DAVHEX010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.111"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..1901
                     /gene="lanM"
                     /locus_tag="VVL45_002114"
     CDS             <1..1901
                     /gene="lanM"
                     /locus_tag="VVL45_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 2 lantipeptide synthetase LanM"
                     /protein_id="HEU4188227.1"
                     /translation="DMPVIIDFETMLQQPLFDDKTGQSLLDTLFHRVTRTLLLPTEGV
                     KREDGLDVEMSALTGNFKKDAFNGQVLINLNTDKVKFDIGKIDFEGGKNLPVRDGDIE
                     FDKYIKDFKKGFRDFYLIFEELNKTEEFKMLLKANLYGLKTRVLFRDTNSYASVLSFL
                     YHPDFYEEMLDREKALENLWSNKFSNQGIVASECEQMRLLDIPIFYTDTNINEIYDDF
                     GNHFISLHQFSGYDYFMKYLPNNNLENLEKQLDIIEIYFPSFDQRKKGYKLNLSNRKK
                     LPNESLVTILHNEVENIADSLVINNIYDEYPIWSIPVNFKGSKWCLEQTGNDMYRGRA
                     GILLFMYYVKLLKDKSSYTQLYNKMLSAIPPSVSLSKTTFGLTGDIGYFIVLSIIEDY
                     DTNTLACLNYIKSVLTELEKTDFASVSNKSDYINGLLPLINTLVRYYRRGIEKERVLR
                     LVLSLGDTLYNDVSERDNSNFGYSFGHGLSGVIFTLKNIYKVTRLKKYKELILQLVPR
                     EKIKIHSLKWCSGEFGTLVNHPQSVPEGIVASLDNDTFCHGSMAIINFYIDYMKEMDT
                     SMELESLLCSVVMNKSKEGEYRIVQTSNFPDFSLYTGVTGIAYTFLRYIALNQNDKKI
                     VIPSLLEI"
BASE COUNT          698 a          231 c          361 g          667 t
ORIGIN      
        1 gagatatgcc tgtaataata gactttgaaa caatgttaca gcaacctcta tttgatgata
       61 aaactggtca gagtttgctg gataccctgt ttcacagggt gactcgaact ttgcttcttc
      121 caacagaggg agtaaaaagg gaggatggtc ttgatgttga gatgagtgct cttacaggaa
      181 attttaagaa ggatgctttt aatggtcaag ttttaattaa tttaaatact gataaggtaa
      241 agtttgatat tgggaaaatt gattttgaag gtgggaaaaa cctgcctgtt cgggatggtg
      301 atatagaatt tgataagtat ataaaagatt tcaaaaaagg ttttagggat ttttatctga
      361 tatttgaaga gttaaataaa acagaagaat ttaaaatgct attaaaagca aacttgtatg
      421 gtttaaagac aagggtactt tttagagaca caaatagcta tgcttcagta ctatcttttt
      481 tatatcatcc tgatttttat gaagaaatgt tagatagaga aaaagcattg gaaaatctct
      541 ggagcaataa gttttctaat caaggtattg tagcaagcga atgtgaacaa atgaggttat
      601 tagatattcc gattttttat acagatacaa atatcaatga aatctacgat gattttggaa
      661 atcattttat ttccctacat caattttctg gatatgacta ttttatgaaa tatttaccta
      721 ataataattt ggaaaattta gaaaagcaac ttgatatcat tgaaatttat tttccaagct
      781 ttgatcagcg aaaaaaagga tataaattaa acttatctaa tagaaaaaaa ctcccaaatg
      841 aaagtttagt tactattctg cataatgaag tagaaaatat tgcagactct ttggttatta
      901 ataatattta tgacgaatat ccaatttgga gtatacctgt taattttaaa ggttctaaat
      961 ggtgtcttga acaaacgggt aatgatatgt ataggggtag agctggaata ttactgttta
     1021 tgtactatgt aaaattattg aaagataaat catcatatac tcaactgtac aataaaatgt
     1081 tatctgcaat tccgccatct gtttctttat ctaagaccac atttggttta actggggata
     1141 taggatattt cattgttcta tccattatag aggattacga tacaaatact ctcgcatgct
     1201 taaattatat aaagagtgtt ttaactgagc ttgaaaagac tgattttgca agtgtttcaa
     1261 acaagtctga ctatattaat ggtcttctac ctttaattaa tacactggta agatattata
     1321 ggagaggaat agagaaggaa cgtgttctaa ggctagtatt aagtttaggt gatacgctat
     1381 ataatgatgt aagcgagaga gataatagta attttggata tagttttggc catggactat
     1441 caggtgtaat ttttacatta aaaaatattt ataaagtgac aagacttaaa aaatataaag
     1501 aattaatttt acaactagtt cctagggaga aaatcaaaat tcattctttg aagtggtgta
     1561 gtggagaatt tgggacatta gttaatcatc cccaaagtgt tccagaggga attgtagcta
     1621 gtttagataa tgatacattt tgccatggaa gtatggccat aataaatttt tacatcgact
     1681 acatgaaaga aatggatact agtatggaat tggagtcttt attgtgtagt gttgtaatga
     1741 ataaatcaaa agaaggagag tatcgtatcg tccaaacaag caatttccca gatttttctt
     1801 tgtatacagg tgtgactggt attgcttata cttttttaag gtatatcgca ctgaatcaga
     1861 atgacaaaaa gatagttatc ccctcattac ttgaaatcta gcttaattta tagttttgtt
     1921 tctaaaatag aggtgtccac tcattaagca atataaa
//
LOCUS       DAVHEX010000111         1947 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVHEX010000111 DAVHEX010000000
VERSION     DAVHEX010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.112"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            79..530
                     /locus_tag="VVL45_002115"
                     /pseudo
     CDS             79..530
                     /locus_tag="VVL45_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013080.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
     gene            695..994
                     /locus_tag="VVL45_002116"
     CDS             695..994
                     /locus_tag="VVL45_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEU4188228.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKASVDEANIKTLYSEKLIGISIPIK"
     gene            complement(1103..>1947)
                     /locus_tag="VVL45_002117"
     CDS             complement(1103..>1947)
                     /locus_tag="VVL45_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEU4188229.1"
                     /translation="QELMTEFSLDILLKAIKLARSTYYYHLKQLDKTDKDQELKAEIQ
                     SIFIEHKGNYAYRRIYLELRNRGYLVNHKRVQGLIKVLNLQAKMRQKRKYSSHKGDVG
                     KKAENLIQGQFEGSKTMEKCYTDVTEFAIPASTQKLYLLPVLDGFNSEIIAYNLSTSP
                     NLEQVQTMLEQAFTEKHYENTILHSDQGWQYQHDSYHQFLEGKGIQASMSRKGNSPDN
                     GMMEFFFGILKSEMFYGYEKSFQSLKQLEQAIVDYIDYYNNKRIKVKLKGLSLVQYRT
                     KSFG"
BASE COUNT          624 a          370 c          339 g          614 t
ORIGIN      
        1 aagattatac cacattgtgt actatttttg gttcatttca ctataataat aacgatacta
       61 gataatgagg aaaatgcaat ggcaaaccca actttcggag aaaaaaagtg aatacagact
      121 atattgctcg ctatggcgta tatgcagtta tccctaatcc tgaacaaaaa caaattgttc
      181 ttgttcaaga accaaatggt gcttggttcc taccatgtgg agaaattgaa gcaggtgaaa
      241 atcatcagga agccctaaag catgagttga ttgaagagct tggtttcaca gcagaaattg
      301 gtacctatta cggacaagct gacgaatatt tctattctcg tcatcgtgat acctactact
      361 acaatcctgc ctacctctat gaagcaactc ctttcaaaga agtacaaaag ccactagaaa
      421 actttaatca tattgcctgg ttccctattg acgaggctat caaaaacctt aaacgtggta
      481 gccataaatg ggccattgaa tcttggaaaa aacagcataa gattggctaa tactcttcga
      541 aaataaaatt caaaccacgt cagcgtcgcc ttaccgtact caagtatagc ctgcggctag
      601 cttcctagtt tgctctttga ttttcattga gtataaatca agcgacttta ttcaaaaagt
      661 gctataatag aattagaaaa tcggaggaaa tattatgaag cttatcaata cgacaaactc
      721 acactcgcaa cttgtaaaaa gtcagctaga aagtaccgat gcaacccttg ttgaggttta
      781 ttctgcaggg aatacagatg ttatttttac ccaagctccg cttcactatg aaatcctcat
      841 ctcaaacaaa caccgtgcta ttcgtgagcc tgaaatcgaa accattcaag aatttttctt
      901 gaaacgtaaa attgataagg cctctgttga tgaagccaat atcaagacac tctactcaga
      961 gaaattaatt ggaatttcaa tcccaatcaa gtaagaaaat tgagtaaaaa ctcgatttga
     1021 gagaaagtcc aataagtctt tcaataataa aacgcatagt agcaagagtt ttgtactgac
     1081 cccaaaaagt tagacaatta atttatccga aggatttagt tctgtattgc acaaggctaa
     1141 gtccttttag ttttacctta attcgtttat tgttgtagta atcaatatag tctacaatgg
     1201 cttgttccaa ttgcttaagc gactgaaacg acttctcata accgtaaaac atttccgatt
     1261 tcagaatccc aaagaagaac tccatcatac cattgtctgg gctgttaccc ttacgtgaca
     1321 tggatgcttg aattccctta ccctcaagaa actgatgata agaatcgtgt tgatattgcc
     1381 agccttggtc actatggaga atcgtattct cgtagtgctt ctctgtgaat gcctgttcca
     1441 acattgtttg tacttgttct aagttgggtg aagttgaaag attataggcg ataatttcgc
     1501 tattaaagcc atctaaaact ggtaataagt aaagcttttg agtacttgct ggaatggcaa
     1561 attctgtcac atctgtgtag cacttttcca ttgttttaga gccttcaaat tggccttgaa
     1621 tgagattctc tgctttctta ccaacgtctc ctttatgaga agaatatttt cgtttttgtc
     1681 gcattttagc ttgtaaattg agtactttta tcaagccttg aactctttta tgatttacca
     1741 gataaccacg atttcttagt tctaaataaa tccgacgata agcataattt cccttgtgtt
     1801 cgataaaaat ggattgaatt tcagctttaa gctcttggtc cttatctgtt ttatctagct
     1861 gtttcaagtg atagtagtag gtcgaacgag ctagtttaat ggcttttaga agaatatcta
     1921 acgaaaactc agtcattaat tcttgaa
//
LOCUS       DAVHEX010000112         1826 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVHEX010000112 DAVHEX010000000
VERSION     DAVHEX010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.113"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            4..216
                     /locus_tag="VVL45_002118"
     CDS             4..216
                     /locus_tag="VVL45_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HEU4188230.1"
                     /translation="MANFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVRYGIGCHRD
                     FIVYHYRVAYRLYLEKLVMNRGFISC"
     gene            447..1733
                     /locus_tag="VVL45_002119"
     CDS             447..1733
                     /locus_tag="VVL45_002119"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEU4188231.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
BASE COUNT          512 a          258 c          426 g          630 t
ORIGIN      
        1 cagatggcta atttctatga tagtattgaa gaagagtatg gtcctacatt tggtgataat
       61 tttgactggg aacatgttca ttttaaattt ttaatttatt atttagtgag atatggcatt
      121 ggttgtcata gggattttat cgtttaccat tatcgtgttg cttatcgttt gtatcttgaa
      181 aaattggtaa tgaatcgggg ttttatttct tgttgaggta attttagtaa atttccgaac
      241 taatttactc ttttatgtaa agataatagt aaatagctag taatttttct aaatcatttt
      301 ttaatagttg gaaatagcaa atctttctat tgtttcttct tgataaaaag gcgatttttt
      361 attataataa attgtaagat ataattgcag gtgagagtcc tgccatgtat gtgagaaagg
      421 aagagcctga gggctcagac aaaattatga cttcagttgt tgttgtaggt acccaatggg
      481 gtgatgaagg taaagggaag attacagact tcctttcagc gaatgcagaa gtgattgcac
      541 gttaccaagg tggtgataat gctggtcaca cgattgtgat tgacggtaag aaatttaagt
      601 tgcacttgat tccatctggg attttcttcc ctgaaaaaat atctgtcatt gggaatggta
      661 tggttgtaaa tcctaaatct cttgtaaaag agttgagcta tcttcatgag gaaggtgtaa
      721 caactgataa cttgcgtatt tctgatcgtg cgcatgttat tttgccttat catatcgagt
      781 tggatcgctt gcaagaagaa gctaagggcg acaataagat tggtacgaca attaagggaa
      841 ttggtccagc ttatatggac aaggctgctc gtgttggaat tcgtattgca gatcttttag
      901 ataaagatat tttccgtgag cgtttagaac gtaaccttgc tgaaaagaat cgtctttttg
      961 aaaaattgta tgacagtaaa gcgattgttt tcgatgatat ttttgaagaa tattacgaat
     1021 atggtcaaca aatcaagaaa tacgtgatag atacatctgt tatcttgaat gatgcgcttg
     1081 ataatggcaa acgtgtgctt tttgaaggtg cacaaggtgt tatgctagat atcgaccaag
     1141 gtacttatcc atttgttacg tcatcaaacc ctgtagctgg tggtgtgaca attggttctg
     1201 gtgtcggtcc aagcaagatt gacaaggttg taggtgtatg taaagcttat acgagtcgtg
     1261 taggagatgg tcctttccca actgagttgt ttgatgaagt gggagaacgt atccgtgaag
     1321 tgggtcatga gtatggtaca acaactggtc gtccacgtcg tgtaggttgg tttgactcag
     1381 ttgtgatgcg tcatagccgt cgtgtttctg gtattactaa cctttctttg aactctattg
     1441 atgttttgag cggtttggat actgtgaaaa tctgtgtggc ctatgatctt gacggtcaac
     1501 gtattgacta ctatccagct agtcttgagc aattgaaacg ttgcaagcct atctatgaag
     1561 agttgccagg ttggtcagaa gatattaccg gagttcgcaa tttggaagat cttcctgaga
     1621 atgcgcgtaa ctatgttcgt cgtgtgagtg aattggttgg cgttcgtatt tctactttct
     1681 cagtaggtcc tggtcgtgaa caaacaaata ttttagaaag tgtttggtcc taagagattt
     1741 ttaagatttg tttaagatag gtcgggtata ctatagacgg ttacaagaag acctcctaac
     1801 ttgttgtaac aaatatccta aacttt
//
LOCUS       DAVHEX010000113         1794 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVHEX010000113 DAVHEX010000000
VERSION     DAVHEX010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.114"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(<1..1613)
                     /locus_tag="VVL45_002120"
     CDS             complement(<1..1613)
                     /locus_tag="VVL45_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec-dependent serine-rich glycoprotein
                     adhesin"
                     /protein_id="HEU4188232.1"
                     /translation="MFKVMRGGVDTTQVMTETVEDKVSHSITGLDILKGIVAAGTVIS
                     GTVATQTKVFTNESAVLEKTVEKTDALATNGTVVLGTISTSNSASSTSLSASESASTS
                     ASESASTSASTSASTSASESASTSASTSISASSTVVGSQTAAATEATAKKVEEDRKKP
                     ASDYVASVTNVNLQSYAKRRKRSVDSIEQLLASIKAAVFSGNTIVNGAPAINASLNIA
                     KSETKVYTGKGVDSVYNVPIYYRLTVTNDGLKLTFTYTVTYVDPKTKTLGNISKKMSN
                     GYSIYNTGTSIQTMLTLGSGLGTPSDVKNSITNKNGGQVQYYNTSTMTKRGSRYTWGN
                     GAQMNGWQAKREYGLTSSWTVPITGTDTSFTFTPYAAKTDNIRTNYFNGRGKVVESST
                     TSQSLSQSKSLSVSASQSASASASTSASASASTSASASASTSASASTSASASASISAS
                     ESASTSASASASTSASASASTSASASASTSASASASTSASESASTSASESASTSASAS
                     ASTSASASASTSASVSASTSASESASTSAS"
     gene            complement(1611..1724)
                     /locus_tag="VVL45_002121"
                     /pseudo
     CDS             complement(1611..1724)
                     /locus_tag="VVL45_002121"
                     /inference="COORDINATES: protein motif:HMM:TIGR03715.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="KxYKxGKxW signal peptide domain-containing
                     protein"
BASE COUNT          458 a          379 c          399 g          558 t
ORIGIN      
        1 gaagcacttg ttgatgcgga ttcagaggca ctggttgacg ctgagactga agcactggtt
       61 gaggctgagg ctgaggcgct agtacttgct gaggctgagg cactggttga tgccgattca
      121 gatgcactgg ttgaggccga ttcagacgct gaggtacttg ctgaagctga cgcactggtg
      181 cttgctgaag ctgaagcact cgttgatgcg gaagctgagg cgctggtact tgctgaggct
      241 gaagcactcg ttgatgccga ttcagacgct gagatacttg ctgaggctga cgcactggtt
      301 gaagccgacg cactggtgct tgctgaagct gaggcgcttg tgcttgctga ggctgaggca
      361 ctggttgatg ctgaggctga ggcgctttga ctagcactta ctgagagtga cttagactgt
      421 gaaagtgact gactggtcgt gctagattca actacctttc ctctaccatt gaagtagtta
      481 gttctaatgt tatctgtttt agcagcgtaa ggggtaaatg taaaggatgt atccgttcca
      541 gtaattggta cagtccaaga tgatgttaat ccatattctc tcttagcctg ccaaccattc
      601 atttgggcac catttcccca agtataccta ctaccccgct tcgtcattgt agatgtatta
      661 tagtattgaa cctgtccacc atttttgtta gtaatggagt tctttacatc tgaaggggta
      721 ccaagaccac tgccaagggt taacattgtt tgtattgaag ttcctgtatt atagatagaa
      781 tatccattac tcatcttttt tgatatatta ccaagagtct ttgttttagg atccacatac
      841 gtaaccgtat aggtaaaggt cagttttaag ccatcatttg tcactgtcaa tcgatagtaa
      901 attggaacat tatataccga atctacacct ttacctgtat aaacttttgt ctcactttta
      961 gcaatgttta gacttgcatt aattgcaggg gcgccattta caatcgtatt gccagaaaaa
     1021 acagcagctt ttatagaagc cagcaattgc tcgatggaat ccactgaacg cttgcgtcgc
     1081 ttagcataag attggagatt gacatttgta actgatgcta cataatcact agctggtttc
     1141 ttacgatctt cttcgacctt cttagcagtt gcttctgtag cggcagctgt ttgtgatcct
     1201 accacagtag atgatgcaga gatacttgtc gaagccgatg tacttgctga ttctgatgca
     1261 cttgtactcg ctgaggtcga agcgctggtt gaggctgact cagatgcaga tgtacttgcc
     1321 gactctgaag ctgacaaact agttgaactc gctgaattac ttgtagatat cgtacctaga
     1381 actactgtac catttgttgc caaagcatcc gttttctcta cagttttttc aagtactgcc
     1441 gactcatttg taaatacctt cgtttgagtt gcaacggttc cacttatgac agttcccgca
     1501 gcaactatcc ccttgaggat atcaagccca gtaattgaat gacttacttt atcttctacc
     1561 gtttcggtca tgacctgagt agtatcaaca ccaccacgca tgaccttaaa caagccaaaa
     1621 agagaagtcg aggcccgtag ccaatgcttc cctgatttga ttaatttgaa ccgagttact
     1681 cggtctgttt ctctatattc acccttctga cgtctaaaaa atattttgaa atttcctttc
     1741 gagtattatt ttcatgtatg gatataaata aattaccata ctataattat agaa
//
LOCUS       DAVHEX010000114         1726 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVHEX010000114 DAVHEX010000000
VERSION     DAVHEX010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.115"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            45..1589
                     /locus_tag="VVL45_002122"
     CDS             45..1589
                     /locus_tag="VVL45_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4188233.1"
                     /translation="MLIYAATINALVLNELIAMNLERFLKLSIYQMIVWCGIIFLDWV
                     VKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHSKSSGTYLSWLNNDVQTLNDQAFKQ
                     LFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLMIMLLVPKIFASKMREVSLNLTNQN
                     EAFLKSSETILNGFDVLASLNLLYVLPKKIKEAGILLKMVIQRKTTVETLAGAISFFL
                     NIFFQISLVFLTGYLAIKGIVKIGTIEAIGALTGVIFTALGELGGQLSSIIGTKPIFL
                     KLYSINPIESNKMNDIEPNEVNRDFPLYEAKNICYKYGDKEILKNLNFCFQRNEKYLI
                     LGESGSGKSTLLKLLSGFLRDYSGELRFCGDDIKKTSYLNMVSNVLYVDQKAYLFEGT
                     IRDNILLEENYTDEEILQSLEQVGLSVKDFPNNILDYYVGDDGRLLSGGQKQKITLAR
                     GLIRNKKIVLIDEGTSAIDRRTSLAIERKILDREDLTVIIVTHAPHPELKQYFTKIYQ
                     FPKDFI"
BASE COUNT          599 a          203 c          325 g          599 t
ORIGIN      
        1 aaaaatttag tttattaacg atatttattg ttgctggtca attattgcta atttatgcag
       61 caactattaa tgctctggtg ttgaatgaat taattgcgat gaatttagag cggtttttga
      121 aattgtcaat ctaccaaatg attgtctggt gtgggataat attccttgac tgggtagtga
      181 aaaattatca ggttgaagtg atccaagagt ttaatctaga gattcgaaat agagttgcca
      241 cagacatctc taactctacc tatcaagaat ttcatagtaa atcatcagga acatatcttt
      301 cgtggctaaa taatgatgtt cagactttaa atgatcaggc gtttaaacaa ctttttttag
      361 taataaaagg aatttctggt actatatttg cagttgtgac tcttaatcac tatcattggt
      421 cattgactgt agccaccttg ttttcattaa tgattatgct acttgtacca aaaatctttg
      481 catcgaaaat gcgagaagtt agtctaaatt taactaacca aaatgaagct tttttaaaat
      541 ctagtgagac tatattgaat ggatttgatg tgttagcgtc cttgaatctt ttatatgtat
      601 tgcctaagaa aattaaagaa gcaggaattt tattaaagat ggttatacaa agaaagacaa
      661 ctgtagaaac gttagcaggc gctattagct tctttctcaa tatttttttt cagatatctc
      721 tcgttttttt aacaggctat cttgcaataa aaggaatagt gaaaattggt actattgaag
      781 caataggagc actaacaggt gttattttta cagcgctagg tgaattagga ggtcaattat
      841 cctctattat tggtacgaag cctatttttt taaaattgta ttcaattaat ccaattgagt
      901 caaataaaat gaatgatatc gaaccaaatg aggtgaatag agattttccg ttatatgaag
      961 caaaaaatat ttgctataag tatggagata aagaaatatt aaaaaactta aatttttgtt
     1021 ttcaacgtaa tgaaaagtat ttaattttag gtgaaagtgg aagcgggaaa tctacattat
     1081 taaaattatt gagtggcttt ttgagagatt atagtggaga attgcgattc tgcggggatg
     1141 atataaaaaa aacctcctat ttaaatatgg tttcgaatgt tctatatgta gatcaaaaag
     1201 cttatttgtt tgaaggtacg attagagata atattttatt ggaagaaaat tatactgatg
     1261 aagaaatact acagtcttta gagcaagttg gtttgagtgt aaaagatttt cctaataaca
     1321 ttttagatta ttatgttggt gatgatggga gattactgtc aggagggcag aaacaaaaaa
     1381 ttactttagc tagagggcta attagaaata agaaaatagt attaattgac gagggaactt
     1441 ctgctatcga taggagaact tcgttagcga ttgaacgtaa gatattagat agagaggatt
     1501 tgactgtcat tattgttacc catgctccgc atccggaact taaacaatat tttactaaga
     1561 tatatcaatt tccaaaggat tttatttaag aatatagggg agggaaacat gagattctat
     1621 tgtgaagctt atgaggtaat tactgaagag aaaaaagtat cattttttta tgtagatgaa
     1681 aaacccttaa ggtatctgtt atttgactat atatttctac tagttc
//
LOCUS       DAVHEX010000115         1694 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVHEX010000115 DAVHEX010000000
VERSION     DAVHEX010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.116"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(96..872)
                     /locus_tag="VVL45_002123"
     CDS             complement(96..872)
                     /locus_tag="VVL45_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU4188234.1"
                     /translation="MFAHLDNSVFLKRNKIWKKTLTYPVEREEITYKRKKAKGKRQAL
                     LAQFDSEEVHHQVEESICPDCQGDLKEIGATLQRQELVFIPAQLKRIDHIQHAYKCQA
                     CSDKNPSDKIVKAPIPKAPLAHSLGSASIIAHTIHQKFNLKVPNYRQEEDWAKMGLPI
                     TRKEIANWHIKASQYYLEPLYNLLREKLLEQALLHADETSYRVLESDSQLPYYWTFLS
                     GKAENQAITLYHHDQRRSGLVVQEFLGDYSGYVHCDMLRQ"
     gene            complement(796..990)
                     /locus_tag="VVL45_002124"
     CDS             complement(796..990)
                     /locus_tag="VVL45_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU4188235.1"
                     /translation="MKIIQQQSATIDSLTNELALLREQVAYLTQKLYGKSSEKSVCPS
                     GQLSLFEEEQNMEEDSDLPS"
     gene            complement(1067..1407)
                     /gene="tnpB"
                     /locus_tag="VVL45_002125"
                     /pseudo
     CDS             complement(1067..1407)
                     /gene="tnpB"
                     /locus_tag="VVL45_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(1388..1573)
                     /locus_tag="VVL45_002126"
     CDS             complement(1388..1573)
                     /locus_tag="VVL45_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188236.1"
                     /translation="MPQPIVPVEIPQSRRFDSKKRNDILLKIRIGKLEVSFFQSLNLE
                     MVEQLLDKVLLYDNSSI"
BASE COUNT          495 a          347 c          317 g          535 t
ORIGIN      
        1 tctagctgtt cgcagtttac caagcttagc gtcgccttac tcctaaacct tggactgcta
       61 tcgcataggc agaactagag gactaaagtc ctaagttact gccgcaacat gtcacaatga
      121 acatagccag aataatctcc taggaattct tgtactacta aaccactccg acgctgatca
      181 tggtggtaca gcgtgattgc ttgattctca gctttcccag acaaaaaagt ccaatagtaa
      241 ggcaactgac tatcactctc tagaacccga taagaggttt catccgcatg aagaagagct
      301 tgttctaaca acttttctcg taaaagatta taaaggggct ccaaatagta ttgactcgcc
      361 ttgatatgcc aattagcaat ttccttacgt gtgattggta aacccatctt agcccaatct
      421 tcttcttggc gataattggg taccttcaga ttaaacttct gatggatggt gtgagcgata
      481 atagaagctg agccaaggct atgcgccaaa ggggctttag gaataggagc tttcacgatt
      541 ttatcactcg gatttttatc actgcatgct tggcacttat aagcgtgttg gatatgatct
      601 attcgtttta attgcgcagg aataaagact aattcttgtc gttgaagggt tgctccaatc
      661 tcttttagat ctccctgaca atcagggcaa atgctctctt ctacttgatg atgaacttct
      721 tctgaatcaa attgggcaag aagagcttga cgtttccctt tagctttctt acgtttatag
      781 gtgatttctt ctctttcaac tgggtaagtc agagtcttct tccatatttt gttcctcttc
      841 aaaaagactg agttgtccag atgggcaaac acttttctca gaggattttc catagagctt
      901 ttgcgttaga taagccactt gttcacgaag aagggcaagt tcattggtga gactatcaat
      961 tgtagcactc tgttgttgaa tgattttcaa tcttcttcca tgggcttcct ctccttattt
     1021 cctaacttta ttataactaa aaagaaagtc ctcatttcaa tagaaatcac gactttctga
     1081 taaatttatt tttggagtga tagaaaagcc cttcataagc caatctactt gttcaggtgt
     1141 gagagctttg acatcctttt ctgtactggg ccaagtcagt tttccgttct caaagcgttt
     1201 atatagtagc caaaatcctt gaccatccca gtaaagggct ttaaagcggt ctttacgtcc
     1261 accacaaaag agaaaaactt gaccggagaa aggatctaat tcaaagtggg ttttaaccag
     1321 ataagccaat gaatcaatgc cttgcctcat atccgttttc ccacatacga gatagacctg
     1381 ccctaggcta gatagatgaa ttgtcataga gcaacacttt atccaaaagc tgttctacca
     1441 tttcgagatt gagagattga aaaaaactta cttcaagctt gccaatacga attttaagca
     1501 gaatatcatt tctcttttta gaatcaaaac gacgagattg tggaatctct acaggaacaa
     1561 taggttgtgg cattaaaata tcctccaata gttttacttt actaatagta tacaggagga
     1621 actccatgaa tgatagatac cttgttatga cgcgattact atattagaaa gttggctctc
     1681 taattaaatt aatt
//
LOCUS       DAVHEX010000116         1658 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVHEX010000116 DAVHEX010000000
VERSION     DAVHEX010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.117"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(<1..>1658)
                     /locus_tag="VVL45_002127"
     CDS             complement(<1..>1658)
                     /locus_tag="VVL45_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S74"
                     /protein_id="HEU4188237.1"
                     /translation="NRLTEGLRTKVDISALNVTAENIRQSVKSLETDTQNKLNQKLSQ
                     AEFEVRAGSIRQEILNATKDKASKSELTQTAEELASKIASVHLGRRNLLKGTKELARY
                     KPVSEYNGFKVIRTVAGATRYQDSYVERTVIPTAGTEYIAIFYARASENDYPVRCHFY
                     NPNTVVSSENSSGYKSRSSDGLSIIRLSTDWQLCWVKWTQTATDQAKTVIIGRHGPQV
                     GGKEGVWVEICAPAIFEGNLAGDWSPAYEDQDERVSAVESNFKQRADSLEAGVSRLTE
                     GLRTKADISSLNVTAENIRQSVKSLETDTQNKLNQKLSQAEFEVRAGSIRQEILNATK
                     DKASKSELTQTAEELSSKIASVQVGGRNYIRGTKRMMLARGLWASGTFRPSGAGTAKT
                     IDVSDSPVTGFDKAIRLTSSNARDQIGIAQDGFYISQGTYTMSCWVKGRRGQKVKLQT
                     YWQVNDNSGISPIFTLKDENWTKLSFTSARNRAGVASIGYVYLVNAEVGEYLDVLAPQ
                     LEDGSLATSSKEAPEDIEGQISTVESTFKQRANSLDAGVRSLTEG"
BASE COUNT          371 a          432 c          343 g          512 t
ORIGIN      
        1 ccttcagtca agcttctcac accagcgtcg agcgagttgg ctcgttgttt aaaggtcgat
       61 tctactgttg aaatctgacc ttctatatct tcaggagctt cttttgagct tgttgccaaa
      121 cttccgtctt ccagctgggg cgcaagaaca tctaaatatt ctccgacttc agcatttacg
      181 agatacacat agccaattga tgcgactcca gccctatttc tagcgctagt aaacgatagc
      241 tttgtccaat tttcatcctt taatgtaaag atgggcgaaa tacccgaatt atcattgact
      301 tgccaataag tttgtagctt gaccttttga cctcttctgc ctttgaccca acaagacatc
      361 gtgtatgtgc cttgcgagat ataaaatcca tcttgagcaa tacctatttg gtctctagca
      421 ttgcttgagg tcaatcgtat cgctttatca aagccagtta ctggactgtc tgatacatca
      481 atcgtctttg ccgtcccagc gcctgacggt ctaaaagtac ctgatgccca caatcctctg
      541 gctagcatca tgcgctttgt accccggata taattcctac ccccgacctg cacactcgct
      601 atcttactag agagctcctc agctgtctgc gtgagttctg acttgctggc tttatccttg
      661 gttgcattca ggatttcctg acgaatagag ccggctcgca cctcaaattc agcctgactc
      721 aacttctgat ttagcttgtt ctgcgtgtct gtctcaagac tcttcacaga ttgccggata
      781 ttctcagcag ttacgttgag tgagctgata tcggctttgg ttctaaggcc ttcagtcaga
      841 cggcttacac cagcttcgag tgaatcagca cgctgtttaa agttggattc gaccgctgag
      901 acacgttcgt cttggtcttc gtatgctggt gaccagtcac ctgcaagatt tccctcaaaa
      961 atggcagggg cgcagatttc aacccatacc ccctctttac cgcctacttg agggccatgg
     1021 cggccaatga tgaccgtctt ggcttgatct gttgcggttt gggtccattt aacccagcac
     1081 aactgccagt ctgtcgagag acggataata gacaagccat ctgacgacct tgacttatat
     1141 ccgctgctgt tttctgatga tacaaccgtg ttaggattgt aaaaatgaca gcgcacagga
     1201 tagtcatttt cactggctcg tgcataaaag atagctatat actctgtccc agccgttggt
     1261 ataacggttc tttccacata gctatcctga tatctagttg ctcctgcgac tgttctgata
     1321 actttaaaac cattatattc actaaccggc ttgtatctcg caagctcttt tgtgcctttc
     1381 agcagatttc tgcgccctaa gtgcacactc gctatcttac tagctagctc ctcagctgtc
     1441 tgcgtaagtt ctgacttgct ggctttatcc ttggttgcgt tcaggatttc ctgacggata
     1501 gagccggctc gcacctcaaa ttcagcctga ctcaacttct gatttagctt gttctgcgtg
     1561 tctgtctcaa gactcttcac agattgccgg atattttcag cagtcacgtt gagtgcgctg
     1621 atatccactt tggttctaag gccttcagtc agacgatt
//
LOCUS       DAVHEX010000117         1618 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVHEX010000117 DAVHEX010000000
VERSION     DAVHEX010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.118"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            1..854
                     /locus_tag="VVL45_002128"
                     /pseudo
     CDS             1..854
                     /locus_tag="VVL45_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            847..>1618
                     /locus_tag="VVL45_002129"
     CDS             847..>1618
                     /locus_tag="VVL45_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU4188238.1"
                     /translation="MDRNIDQELVSIIIPTHNRYESLIRAVKSCLHQSYKNIEVIIID
                     DNYSNVNLRNKIIHQFGYTNHRIKLILSNEDLGATNARNIGIKNSRGKYISFLDDDDE
                     YMPDRILKLMACFKKSRMKNLALVCSYGIIIYPNGTREEEKTDFVGNPLFVQMVHNIA
                     GTSFWLCKKEVLELINGFEKIDSHQDGVVLLKLLVQGYQIDIVREFLVNYYAHSKENG
                     ITGVTQKTINADEEYYNYCRKYFNLLSFNERILVTKKYY"
BASE COUNT          605 a          149 c          324 g          540 t
ORIGIN      
        1 attttttata tatgttaaaa aaacttgaca agaatggatt tggaaaggtt aagacagtga
       61 taacatgcca tggatgggtt gaatataact taaaaaagaa attcttgact tattttgatt
      121 tttggacata ttctatggga gatgctttta tttgtgtatc tgaaactatg aaaaaaaaga
      181 ttggagagta taataaaaaa taagaagata gttgcaataa ataacggaat taatgtatca
      241 aatagcgatt tagatgtagt aggtgttcaa gactttaaaa aagaattttg tattccaaat
      301 aacaaaaaat catttgttat gttggaaggt tggatccaga aaaaaggccg gatagattcc
      361 ttgaatttgc agaaaaatta tttttagtca gagaagatgt gatttttatt atggctggaa
      421 atggtagtat gtgggctgct ttgaaagaaa aaatttgcca tttgaaatgt agagataatt
      481 tcaggttact tggtgaaatc tatccagcta ctatagttta ccaaatatcg gatttattgt
      541 atattccttc tgatacagaa ggaataccta tgtgtgtttt agagtcaatg tcacaaggaa
      601 ctccggtttt agctagtaat gttggcgggt taagtgaaat tattgaacat agggttgatg
      661 gatttttatt tgagaaggaa gatgttgagg gagtgtgtgc ttgtgctaat tttttactca
      721 atgattctga gtatttgaaa tatataggtg agaatagtaa atcaaaaata agaaaacatt
      781 tttctgtgca aaaaatgttt gtagaaacca tgagagtata tgatgaatta ttagagaaga
      841 gtagtcatgg ataggaatat agatcaagaa ttagtaagta ttataatacc aacacataat
      901 aggtatgaat ctctgattag agctgtgaaa agttgtttac atcaaagtta taaaaatatc
      961 gaagttataa ttattgatga taattatagt aatgtaaatt taagaaataa aattatccat
     1021 caatttggct ataccaatca tagaattaag ttaattttaa gtaatgaaga tttaggtgca
     1081 actaatgcca gaaacatagg tatcaaaaat tctagaggta agtatatatc atttttagac
     1141 gatgatgatg aatatatgcc agatcgaatt ttaaagttga tggcttgttt taaaaagagt
     1201 agaatgaaga atttagcttt agtttgtagt tatggaataa taatttatcc aaatggtaca
     1261 cgagaagagg agaagaccga ttttgttgga aatcccttgt ttgttcaaat ggttcacaat
     1321 atagcaggta cgtcattttg gttgtgtaaa aaagaggtgc tagaattaat taatggtttt
     1381 gagaaaatag attcacatca ggacggtgtt gttttattaa aactacttgt tcaaggatac
     1441 caaattgata tagtgcgaga attcttggtg aattactacg ctcacagtaa agaaaacggt
     1501 atcactggag tgacacaaaa aacaattaat gcagatgaag aatattataa ttactgtagg
     1561 aaatatttta atttattgag tttcaacgag agaatattgg ttacaaagaa atattatt
//
LOCUS       DAVHEX010000118         1611 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVHEX010000118 DAVHEX010000000
VERSION     DAVHEX010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.119"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..319
                     /locus_tag="VVL45_002130"
     CDS             <1..319
                     /locus_tag="VVL45_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEU4188239.1"
                     /translation="AIGLGANPELSRLVLIFCAVSLTAFSTSITGPIACISFLAGPIA
                     LNIGKKRSPILAGLVGILLVLLSDIFSQNILPARYPVGVVTGLLGSPYLIYLLIKMNR
                     RNI"
     gene            319..1113
                     /locus_tag="VVL45_002131"
     CDS             319..1113
                     /locus_tag="VVL45_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4188240.1"
                     /translation="MKGLWSNNLTCGYDEKIILENINIKIPEEKISVIIGSNGCGKST
                     LIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPIIPESITVADLVSRGR
                     FPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGGQRQRVWIALALAQDTSI
                     LLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKL
                     VAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKHHVNS"
BASE COUNT          630 a          253 c          264 g          464 t
ORIGIN      
        1 agcaatcgga cttggagcaa atcccgagct ttcaaggctt gttttaattt tttgcgctgt
       61 atctttaact gctttttcta cttcaattac aggaccaata gcttgtatat cttttttagc
      121 tggtcccata gccttaaata ttggcaagaa aagaagccca atattagctg gattggttgg
      181 aattttacta gttttgttat cagacatatt ctctcaaaat attttaccag ctagatatcc
      241 agtaggtgtt gtaactggct tgttaggttc accatactta atatacttac taataaaaat
      301 gaacaggagg aatatataat gaaaggtctg tggtcaaata atcttacctg cggttatgat
      361 gaaaaaataa tcttggaaaa tataaatata aaaatacctg aagaaaaaat atcagttatt
      421 attgggtcaa atggttgtgg gaaatcaaca ctcattaaaa ccttgtctcg acttataaag
      481 ccattagagg gagaagtatt gcttgataat aaatcaatta attcttataa agaaaaagat
      541 ttagcaaaac acatagctat attacctcaa tctccaataa tccctgaatc aataacagta
      601 gctgatcttg taagccgtgg tcgtttcccc tacagaaagc cttttaagag tcttggaaaa
      661 gatgaccttg aaataataaa cagatcaatg gttaaggcca atgttgaaga tctagcaaat
      721 aacctagttg aagaactttc tgggggtcaa aggcaaagag tatggatagc tctagcccta
      781 gcccaagata caagtatcct acttttagat gagccaacta cttacttgga tatctcatat
      841 caaatagaac tattagacct cttgactgat ctaaaccaaa aatataagac aaccatttgc
      901 atgattttgc acgatataaa tctaacagca agatacgctg attacctatt tgcaattaaa
      961 gaaggtaaac ttgttgcaga gggaaagcct gaagatatac taaatgataa actagttaaa
     1021 gatatcttta atcttgaagc aaaaattata cgtgacccta tttccaattc gcctctaatg
     1081 attcctattg gcaagcacca tgttaactct taataattga atattggaat tcatttaaaa
     1141 gtcgcaaatt ttgaactgac cctcaaaagt taggccaaaa aactaactaa aggaggtcag
     1201 ttttttaatg gcaaatacag cacagaattt aagataaaat tagtaaaaga atatcatgaa
     1261 tgaaaaatag gatatagaga ataacagcaa tattaaaaag atcaggactg ataataaaca
     1321 aaaagaaagt acaacgacta gttcaaaagc taaaacttca agtaaaaagt tattcaagaa
     1381 aatctagaaa atactcatcc tacaaaggac aggtaggaaa aatatcagac aacaaaataa
     1441 aaagaaatat ttattaccga aaaaaagggg aaatcgagtt aagtaccaac aggctcatct
     1501 attccaaaaa gatatttact cgatggaaaa actcctagaa ttagtgctac aaatattgac
     1561 aatggtatct taggttacta cgaagatata gatgataaaa attatagagt t
//
LOCUS       DAVHEX010000119         1527 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVHEX010000119 DAVHEX010000000
VERSION     DAVHEX010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.120"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(192..953)
                     /locus_tag="VVL45_002132"
     CDS             complement(192..953)
                     /locus_tag="VVL45_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PezT"
                     /protein_id="HEU4188241.1"
                     /translation="MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAG
                     KTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQEYGKDSVEYTKDFAGKMVESLV
                     TKLSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
                     LYIINPNQARATPKEHHDFIVNHLVDNTRKLEELAIFERIQIYQRDRSCVYDSKENTT
                     SAADVLQELLFGEWSQVEKEMLQVGEKRLNELLEK"
     gene            complement(953..1429)
                     /locus_tag="VVL45_002133"
     CDS             complement(953..1429)
                     /locus_tag="VVL45_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU4188242.1"
                     /translation="MIGKNIKSLRKTHDLTQLEFARIVGISRNSLSRYENGTSSVSTE
                     LIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIEIVKERGANLLSRLYRYQDSQGI
                     SIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA"
BASE COUNT          453 a          304 c          208 g          562 t
ORIGIN      
        1 gtaatttttc tatatttcgt tgcagtccaa ttagaaaata gcttgtaatc ataagaaaca
       61 ggtgtatgca aatgaattaa agtcttgtga aagctaccga atttctcttt ggaatttgtt
      121 agttcttgat aaacagactt tatcttgtta ttaaaccctc tcatttctat tctcctaaaa
      181 atatcaattg tttatttttc aagtaattca ttaagtctct tttcccccac ctgcaacatc
      241 tccttctcta cctgactcca ctccccaaag agtaactctt gaagaacatc tgctgctgaa
      301 gttgtatttt cttttgaatc atatacacaa cttctatctc gttggtaaat ttgaattctt
      361 tcaaagatag ctagttcttc caattttcgt gtgttatcaa ctagatgatt tacaatgaaa
      421 tcatgatgtt cttttggagt tgcgcgtgct tgatttggat tgataatgta cagttcttca
      481 taacggataa gagtacttag atacgacaat tcaggctttg tcgcaattaa ggccaattgt
      541 acttcatatc ccttattttt caagagttgt gctgttttct ttggaacatc aactgttcgt
      601 aaagttccct ctatcaaaag attgtatctc aaactactca attttgttac taaagactct
      661 accatttttc ctgcaaaatc tttggtatat tctacactgt ctttgccata ttcttgctgc
      721 agttctaaat agtgtggatg ctgagaacga aaactatcac catctatgat aacaatattt
      781 ccttgaaatt ctttctgttt aatacgatga attgtagtct taccggcacc actttgccct
      841 ccaagcaaaa tcgctatagg ttgcttactg gacttttttc ctcttgtcag tgaacgaaga
      901 ttccttgcta aagcatgttt gaattcacta tcagtataat cttggatttc cattaggcca
      961 ccatccgttt ttcagatatc tctaacatac gttcaattcc atccaaatag ccactatatc
     1021 tctctatttc atcaaaagtt tctactagat agatattcgt atgaatcaaa tcagatagat
     1081 catcactcat taaaatccaa ggattagact catcatcaat gctaattccc tgactatctt
     1141 gataacgata gagtcgagat aatagattag cacctctttc tttcacaatt tcaattttta
     1201 aagtcaattc ataatcttca acaggattga gcattttatc ttctcctaca atatcgacat
     1261 aagatacatt aaacttctga caaatgatgt ctattaattc ggtagagact gaactcgttc
     1321 cattttcata acgactcaga ctatttcgtg aaatacctac aatccgtgca aattcgagtt
     1381 gtgttaagtc atgtgtttta cgcaaggatt ttatgttctt tccaatcata gcaaactcct
     1441 ttatttttat aaatccatta tagcataaaa aagcataacg caccaaaaat ggtgcgttat
     1501 ttcttgtttt tctatcctaa actttac
//
LOCUS       DAVHEX010000120         1501 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVHEX010000120 DAVHEX010000000
VERSION     DAVHEX010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.121"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            145..1491
                     /locus_tag="VVL45_002134"
     CDS             145..1491
                     /locus_tag="VVL45_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSpn5 family transposase"
                     /protein_id="HEU4188243.1"
                     /translation="MNSLPNHHFQNKSFYQLSFDGGHLTQYGGLIFFQELFSQLKLKE
                     RISKYLVTNDQRRYCRYSDSDILVQFLFQLLTGYGTDYACKELSADAYFPKLLEGGQL
                     ASQPTLSRFLSRTDEETVHSLRCLNLELVEFFLQFHQLNQLIVDIDSTHFTTYGKQEG
                     VAYNAHYRAHGYHPLYAFEGKTGYCFNAQLRPGNRYCSEEADSFITPVLERFNQLLFR
                     MDSGFATPKLYDLIEKTGQYYLIKLKKNTVLSRLGDLSLPCPQDEDLTILPHSAYSET
                     LYQAGSWSHKRRVCQFSERKEGNLFYDVISLVTNMTSGTSQDQFQLYRGRGQAENFIK
                     EMKEGFFGDKTDSSTLIKNEVRMMMSCIAYNLYLFLKHLAGGDFQTLTIKRFRHLFLH
                     VVGKCVRTGRKQLLKLSSLYAYSELFSALYSRIRKVNLNLPVPYEPPRRKASLMMH"
BASE COUNT          432 a          335 c          280 g          454 t
ORIGIN      
        1 ctaaaaacca tccagaatcc ttgccttagc ttagatcctg gatggtttct tttttcaccc
       61 aatgggtgtt ttttactaga caaaaaagag tttccccttt atggtataag tgtagaaaaa
      121 aacacaaaaa gaaaggaaac tcacatgaac agtttaccaa atcatcactt ccaaaacaag
      181 tctttttacc aactatcttt cgatggaggt catttaaccc agtatggtgg tcttatcttt
      241 tttcaggaac ttttttccca gttgaaacta aaagagcgga tttctaagta tttagtaacg
      301 aatgaccaac gccgctactg tcgttattcg gattcagata tccttgtcca gttcctcttt
      361 caactgttaa caggttatgg aacggactat gcttgtaaag aattgtcagc tgatgcctac
      421 tttccaaaat tgttggaagg agggcagctt gcttcacagc caaccttatc ccgttttctt
      481 tccagaactg acgaggaaac agtccatagt ttgcgatgcc tcaaccttga attggtcgaa
      541 ttctttttac agtttcacca gctaaaccaa ctcattgtag atatcgattc tacccatttc
      601 acaacttatg gcaagcaaga aggtgttgct tataacgccc actatcgtgc tcatggctat
      661 catcctcttt atgctttcga ggggaagaca ggttattgtt tcaatgccca gcttcgtcct
      721 ggtaatcgtt attgttctga agaggcagac agctttatca cacctgtttt agaacggttt
      781 aatcaacttc tctttcgaat ggatagtggc tttgcgaccc caaaattata cgatttaatt
      841 gaaaaaacag ggcaatacta cctcataaaa ctcaagaaaa atactgttct gagccgtctt
      901 ggagaccttt ccctcccttg cccacaggat gaggacttaa ccatcttgcc ccactccgcc
      961 tactcagaaa ctctctatca agcaggatct tggtcgcaca agcgtcgtgt ctgccagttc
     1021 tctgaacgaa aagaaggaaa cttgttctac gatgttattt ctctcgttac aaatatgacg
     1081 agtggaacaa gccaagacca gtttcagctt tatcgtggac gtggtcaagc cgagaatttc
     1141 atcaaggaga tgaaggaggg attttttggc gataaaacgg atagttcaac cttaatcaaa
     1201 aacgaagttc gtatgatgat gagctgtatc gcctacaatc tctatctttt tctcaaacat
     1261 ctagctggag gtgacttcca aactttaaca atcaaacgct tccgccatct ttttcttcac
     1321 gtggtgggaa aatgtgttcg aacaggacgc aagcagctcc tcaaattgtc tagtctctat
     1381 gcctattccg aattgttttc agcactttat tctaggatta gaaaagtcaa cctgaatctt
     1441 cctgttcctt atgaaccacc tagaagaaaa gcgtcgttaa tgatgcatta aagaacagtc
     1501 g
//
LOCUS       DAVHEX010000121         1475 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVHEX010000121 DAVHEX010000000
VERSION     DAVHEX010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.122"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            72..>1475
                     /gene="pcpA"
                     /locus_tag="VVL45_002135"
     CDS             72..>1475
                     /gene="pcpA"
                     /locus_tag="VVL45_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HEU4188244.1"
                     /translation="MKKTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSII
                     QQNNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVA
                     SQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRA
                     FDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLT
                     LPKSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESY
                     KTPKETKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERL
                     AFYGNLELKELILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEI
                     HIKNKSTEFSVKKDTFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETD
                     VAKPKKNSNQGVVGWVKD"
BASE COUNT          579 a          203 c          235 g          458 t
ORIGIN      
        1 ctttttataa caacggctag caaagttata attttatatc tattagaaaa taaatatgta
       61 aggaaattta aatgaaaaaa actacaatat tatcattaac tacagctgca gttattttag
      121 cagcatatgt ccctaatgaa ccaatcctag cagatactcc tagttcggaa gtaatcaaag
      181 agactaaagt tggaagtatt attcaacaaa ataatatcaa atataaggtt ctaactgtag
      241 aaggtaacat aggaactgtt caagtgggta atggagttac tcctgtagag tttgaagctg
      301 gtcaagatgg aaaaccattc acgattccta caaaaatcac agtaggtgat aaagtattta
      361 ccgttactga agtagctagt caagctttta gttattatcc agatgaaaca ggtagaattg
      421 tctactatcc tagctctatt actatcccat caagcataaa aaaaatacaa aaaaaaggct
      481 tccatggaag taaagctaaa actattattt ttgacaaagg cagtcagctg gagaaaattg
      541 aagatagagc ttttgatttt tctgaattag aagagattga attgcctgca tctctagaat
      601 atattggaac aagtgcattt tcttttagtc aaaaattgaa aaagctaacc ttttcctcaa
      661 gttcaaaatt agaattaata tcacatgagg cttttgctaa tttatcaaat ttagagaaac
      721 taacattacc aaaatcggtt aaaacattag gaagtaatct atttagactc actactagct
      781 taaaacatgt tgatgttgaa gaaggaaatg aatcgtttgc ctcagttgat ggtgttttgt
      841 tttcaaaaga taaaacccaa ttaatttatt atccaagtca aaaaaatgac gaaagttata
      901 aaacgcctaa ggagacaaaa gaacttgcat catattcgtt taataaaaat tcttacttga
      961 aaaaactcga attgaatgaa ggtttagaaa aaatcggtac ttttgcattt gcagatgcga
     1021 ttaaacttga agaaattagc ttaccaaata gtttagaaac tattgaacgt ttagcctttt
     1081 acggtaattt agaattaaaa gaacttatat taccagataa tgttaaaaat tttggtaaac
     1141 acgttatgaa cggtttacca aaattaaaaa gtttaacaat tggtaataat atcaactcat
     1201 tgccgtcctt cttcctaagt ggcgtcttag attcattaaa ggaaattcat attaagaata
     1261 aaagtacaga gttttctgtg aaaaaagata catttgcaat tcctgaaact gttaagttct
     1321 atgtaacatc agaacatata aaagatgttc ttaaatcaaa tttatctact agtaatgata
     1381 tcattgttga aaaagtagat aatataaaac aagaaactga tgtagctaaa cctaaaaaga
     1441 attctaatca gggagtagtt ggttgggtta aagac
//
LOCUS       DAVHEX010000122         1450 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVHEX010000122 DAVHEX010000000
VERSION     DAVHEX010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.123"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            243..1040
                     /locus_tag="VVL45_002136"
                     /pseudo
     CDS             243..1040
                     /locus_tag="VVL45_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1075..>1450)
                     /locus_tag="VVL45_002137"
     CDS             complement(1075..>1450)
                     /locus_tag="VVL45_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630-like element IS630-Spn1 family
                     transposase"
                     /protein_id="HEU4188245.1"
                     /translation="GKVSGRRYQRISLVAGLTNGELIAPMTYEETMTSDFFEAWFQNF
                     LLPTLNTPSVIIMDNVRFHRMGKLELLCEEFGHKLLPLPPYSPEYNPIEKTWAHIKKH
                     LKKVLPSCNTFYEAFLSCSCFN"
BASE COUNT          493 a          288 c          225 g          444 t
ORIGIN      
        1 tataaactta ttctaacaaa aaattttaca cattcactat caattcctga attgttaaaa
       61 taccagcctc aacaagataa aaataggaaa agttgacaaa atagaaacaa atttgctacc
      121 taaatttcaa acaattaaag ttctaaaaag accgccttaa aacttttggg taaatcctat
      181 tagaactata atatttttat ataggtatta tggttctgat tttctagtat aaaaaaattc
      241 aaactagaat tcttcttaga aaaggccgtt ttaagtgcta tcattgctca aaaatggagc
      301 tcgctgagac ttctatcgtc aagaagaatc atcaaattcc ttgtatcatc aaccaaaaga
      361 ttgctcaaaa gttaattgaa aagatttcta tgaccgacat tgatcatcag ttgtctattt
      421 caacttcaac tgtcattcgc aagatcaata attttcactt tgagcatgat ttttcgcgtc
      481 ttcctgagat tatgtcctag gacgttgaaa cagtccgggg agtgactgtt tcaatcggga
      541 gatggagatg agctttattg ctcaggactt tgataaactc aatatcataa ctgttcttga
      601 gagcagaaca caagccatca tccgaaatcc catgaataca aggctatcaa gcgctactgg
      661 aagctcattc aacaagatag tcagaaactg agtgataaac gattttatcg ccctactttt
      721 cgtatgcact tgactaataa agaaattcta gataaacttt tgagctattc cgaagacttt
      781 aaacaccact ataatctcta tcagctcttg cttttccacc tctgtttcag actttgttta
      841 aaaccttcct caaagataaa gagaaaatcg tcaacgccct tcaattacct tattccaacg
      901 ccaaactgga agccaccaat aatctcatca aacttatcaa gcacaatgcc tttggtttta
      961 ggaactttga aaacttcaaa aaagaaagga cgaaatttgt cctttctaga tcttcgcttt
     1021 cttcaaccca ctacagttga caaagaaccg aaaaatgtct cgcctctaat atagtcaatt
     1081 gaaacaagag caggataaaa aagcctcgta aaaggtattg caacttggta ataccttttt
     1141 gaggtgcttt ttgatatgag cccatgtttt ctcaatagga ttgtactcag gcgagtaggg
     1201 aggaagaggt aaaagtttat gcccaaactc ttcgcataaa agttctagct tccccattct
     1261 atggaatctt acattatcca taataataac cgatggtgtg tttaatgttg gtaagagaaa
     1321 attctgaaac caagcttcaa aaaagtcgct cgtcatcgtc tcttcgtaag tcattggagc
     1381 gattaattca ccatttgtta gacctgcaac caaagaaatc ctctgatatc ttcttccaga
     1441 tactttgcct
//
LOCUS       DAVHEX010000123         1416 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVHEX010000123 DAVHEX010000000
VERSION     DAVHEX010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.124"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            252..592
                     /gene="tnpB"
                     /locus_tag="VVL45_002138"
                     /pseudo
     CDS             252..592
                     /gene="tnpB"
                     /locus_tag="VVL45_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849103.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(625..1416)
                     /locus_tag="VVL45_002139"
                     /pseudo
     CDS             complement(625..1416)
                     /locus_tag="VVL45_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          428 a          289 c          255 g          444 t
ORIGIN      
        1 ttaatttcat aataaaaaca ccccaaaaag ttagattttt tctgtctaac tttttggggg
       61 gcagtacact acacctacta ttatacgttt ttatcatttt gaaatctact gctaatcaat
      121 gaagaagcaa agatcaaact aggcagctag ttacaagtta cgtaagcgcg tcataacaag
      181 gtatctatcc taattcccca acttccagta tactagataa aaaataatta tcggaggaaa
      241 ggtattgtac tatgataatt caactaagtg atttaggtca agttcacctt gtttgtagca
      301 agatagatat gaggcaggga atagactcat tagcctatgt agttaaaacc cactttgaat
      361 tggatccttt ctccggtcaa gtctttctct tttgtggtgg acgtaaagac cgctttaaag
      421 ccctttactg ggatggtcaa ggattttggc tactatataa acgctttgag aacggcagac
      481 tgacttggcc cagtacagaa aaggatgtca aagctctcac acctgaacaa gtagactggc
      541 ttatgaaggg cttttctatc actccaaaaa taaatccatc agaaagtcgt gatttctatt
      601 gaaatgagga ctttcttttt atactcatct gctttcaaaa agcattctag tccatctccg
      661 attaacgatg ggctttatca cctccttctc cagtccttgt atgatatctt aaagttgatt
      721 catgacatct tccaaagttt gaaaggcttt atttttaaat ccacgtttac gaatctcttt
      781 ccacacttgt tcaatggggt tcatctctgg tgtgtacgga ggaataaatg caaagccaat
      841 attagtcgga atctttaagg tacttgattt atgccatata gcattgtcca taacgaataa
      901 aagataatca tctggataag cttgtgaaag ctcttctaaa aagacgttca tccactcagt
      961 attacatcca ccagctatta agaaaaatga ttcgcctgta tgggtccata acaatagcga
     1021 aattctcgta tatagtgact atggacatgt ggacctactc ctattggaga ccaacaagat
     1081 cccagtttac tgattctacc gaagccagcc tcatcttggt acatcaagcg aaccttatga
     1141 aaacgtctac tggttttaaa acgctttctt ttcttcttga attgagattt tatttttaga
     1201 cgcgacaatg gtttgagcgt ctgctttctt aggatgttct agacgtggca taatatttcg
     1261 ccaaccatgg cgcttcaaca gttgatagaa ggcatcacgt gtgtaggaac gacctgactc
     1321 ctttttataa gcctgaaata aggcatcaat tgtaacaaat tctcctgcct ctgcagcctt
     1381 caaatgacgg gcaagaaagg ctttctcttc cccaac
//
LOCUS       DAVHEX010000124         1405 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVHEX010000124 DAVHEX010000000
VERSION     DAVHEX010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.125"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            15..>1405
                     /locus_tag="VVL45_002140"
     CDS             15..>1405
                     /locus_tag="VVL45_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate hydrolase"
                     /protein_id="HEU4188246.1"
                     /translation="MNKKKMILTSLASVAILGAGFVTSQPTFVRAEESPQVVEKSSLE
                     KKYEEAKAKADTAKKDYETAKKKAEDAQKKYEDDQKRTEEKARKEAEASQKLNDVALV
                     VQNAYKEYREVQNQRSKYKSDAEYQKKLTEVDSKIEKARKEQQDLQNKFNEVRAVVVP
                     EPNALAETKKKAEEAKAEEKVAKRKYDYATLKVALAKKEVEAKELEIEKLQYEISTLE
                     QEVATAQHQVDNLKKLLAGADPDDGTEVIEAKLKKGEAELNAKQAELAKKQTELEKLL
                     DSLDPEGKTQDELDKEAEEAELDKKADELQNKVADLEKEISNLEILLGGADSEDDTAA
                     LQNKLATKKAELEKTQKELDAALNELGPDGDEEETPAPAPQPEQPAPAPKPEQPAPAP
                     KPEQPAPAPKPEQPAKPEKPAEEPTQPEKPATPKTGWKQENGMWYFYNTDGSMAIGWL
                     QNNGSWYYLNANGA"
BASE COUNT          574 a          242 c          301 g          288 t
ORIGIN      
        1 agaggtaaat ttagatgaat aagaaaaaaa tgattttaac aagtctagcc agcgtcgcta
       61 tcttaggggc tggttttgtt acgtctcagc ctacttttgt aagagcagaa gaatctccac
      121 aagttgtcga aaaatcttca ttagagaaga aatatgagga agcaaaagca aaagctgata
      181 ctgccaagaa agattacgaa acggctaaaa agaaagcaga agacgctcag aaaaagtatg
      241 aagatgatca gaagagaact gaggagaaag ctcgaaaaga agcagaagca tctcaaaaat
      301 tgaatgatgt ggcgcttgtt gttcaaaatg catataaaga gtaccgagaa gttcaaaatc
      361 aacgtagtaa atataaatct gacgctgaat atcagaaaaa attaacagag gtcgactcta
      421 aaatagagaa ggctaggaaa gagcaacagg acttgcaaaa taaatttaat gaagtaagag
      481 cagttgtagt tcctgaacca aatgcgttgg ctgagactaa gaaaaaagca gaagaagcta
      541 aagcagaaga aaaagtagct aagagaaaat atgattatgc aactctaaag gtagcactag
      601 cgaagaaaga agtagaggct aaggaacttg aaattgaaaa acttcaatat gaaatttcta
      661 ctttggaaca agaagttgct actgctcaac atcaagtaga taatttgaaa aaacttcttg
      721 ctggtgcgga tcctgatgat ggcacagaag ttatagaagc taaattaaaa aaaggagaag
      781 ctgagctaaa cgctaaacaa gctgagttag caaaaaaaca aacagaactt gaaaaacttc
      841 ttgacagcct tgatcctgaa ggtaagactc aggatgaatt agataaagaa gcagaagaag
      901 ctgagttgga taaaaaagct gatgaacttc aaaataaagt tgctgattta gaaaaagaaa
      961 ttagtaacct tgaaatatta cttggagggg ctgattctga agatgatact gctgctcttc
     1021 aaaataaatt agctactaaa aaagctgaat tggaaaaaac tcaaaaagaa ttagatgcag
     1081 ctcttaatga gttaggccct gatggagatg aagaagaaac tccagcgccg gctcctcaac
     1141 cagagcaacc agctcctgca ccaaaaccag agcaaccagc tccagctcca aaaccagagc
     1201 aaccagctcc agctccaaaa ccagagcaac cagctaagcc ggagaaacca gctgaagagc
     1261 ctactcaacc agaaaaacca gccactccaa aaacaggctg gaaacaagaa aacggtatgt
     1321 ggtatttcta caatactgat ggttcaatgg caataggttg gctccaaaac aacggttcat
     1381 ggtactacct aaacgctaac ggcgc
//
LOCUS       DAVHEX010000125         1318 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVHEX010000125 DAVHEX010000000
VERSION     DAVHEX010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.126"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            100..282
                     /locus_tag="VVL45_002141"
     CDS             100..282
                     /locus_tag="VVL45_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HEU4188247.1"
                     /translation="MANDNKSHYLIYRVLGISFEEGENIDLYQNKGRFLYKYAGSFLE
                     EAAVLCFNEKFGTENT"
     gene            292..657
                     /locus_tag="VVL45_002142"
     CDS             292..657
                     /locus_tag="VVL45_002142"
                     /inference="COORDINATES: protein motif:HMM:NF021048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HEU4188248.1"
                     /translation="MPNSESTKPKTFEIDCLVGEKHAYEIKWWDATTDGDHITKEHTR
                     IKVIHNKGYIPIRLMFYYPNRTQAIKIQQTLETLYNGIGGKYYYGDSAWEHLRAVTGI
                     DLLSILTDIANKKTGVKSK"
     gene            654..>1318
                     /locus_tag="VVL45_002143"
     CDS             654..>1318
                     /locus_tag="VVL45_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEU4188249.1"
                     /translation="MTVLKGDNLEILKTIESSSIDLIYMDPPFFTQKTQKLSNNKNIM
                     YSFEDTWTSIEDYKEFLSVRLEECKRVLKNSGSIFVHCDKIANHHIRLILDNIFGVDM
                     FQSEIIWNYKRWSNSKKGLLNNHQNIYFYSKSKDFKFNTIFTEYSSTTNIDQILVERK
                     RDGNSKTIYKVDNNGNYILAKEKNGVPLSDVWNIPFLNPKAKERVGYPTQKPILLLEQ
                     IIK"
BASE COUNT          546 a          166 c          212 g          394 t
ORIGIN      
        1 agaggataga aatgaccgaa ttagaaagaa aaaatcgaaa aattagctaa gaaatattct
       61 gataacttaa acatcaaagt tcaagagaga gttcgtgaaa tggcaaatga taataagagc
      121 cattatttga tatacagagt tttaggtatt tcatttgaag aaggagaaaa tatcgatttg
      181 tatcaaaata aaggtcgttt tttatacaaa tatgctggtt catttttaga agaagctgca
      241 gtactatgct ttaacgaaaa atttggtaca gaaaatactt aaaaagttaa cattcctaat
      301 tctgaaagta caaaacctaa gacttttgaa attgattgtt tagtcggaga aaaacacgca
      361 tacgaaataa aatggtggga tgcaactaca gatggagacc atataactaa agaacacact
      421 agaataaaag ttattcataa caaaggatat ataccaattc ggttaatgtt ctactatcca
      481 aatagaactc aagctataaa aattcagcaa actttagaaa cattgtataa cggtattgga
      541 gggaaatatt attatggaga ttctgcctgg gaacatttaa gagcagtgac cggtattgat
      601 ttacttagta ttctaacaga tattgcaaat aaaaaaacag gggtaaaatc aaaatgacag
      661 tattaaaagg agataactta gaaatattaa aaactattga atcctcaagt attgatttaa
      721 tctatatgga ccctcctttc tttacacaga aaacccaaaa attatctaat aacaaaaata
      781 ttatgtattc attcgaagat acgtggactt cgattgagga ttacaaagaa tttttgtctg
      841 taagattaga agaatgcaaa agagtgctaa aaaatagtgg cagtattttc gttcattgtg
      901 ataaaattgc aaatcatcat attagattaa ttttagataa tatctttgga gtagatatgt
      961 ttcaaagcga aattatatgg aactataaac ggtggtctaa ttcaaaaaag ggattattga
     1021 acaatcatca aaacatttac ttttattcaa agtcaaaaga ttttaaattt aatacaattt
     1081 ttacagagta ttcttctact acaaatatcg accaaatact agtggaacga aaacgagatg
     1141 gaaactctaa aactatatat aaggttgata ataatggtaa ctatattcta gcaaaagaga
     1201 aaaatggagt tcccctttca gatgtttgga atataccatt tcttaatcca aaagctaaag
     1261 aaagagtagg ttatcctaca caaaaaccta ttctgttatt agaacaaatt ataaagat
//
LOCUS       DAVHEX010000126         1285 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVHEX010000126 DAVHEX010000000
VERSION     DAVHEX010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.127"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(<1..372)
                     /locus_tag="VVL45_002144"
     CDS             complement(<1..372)
                     /locus_tag="VVL45_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEU4188250.1"
                     /translation="MNDEASKQLTDARFKRLVGVQRTTFEEMLAVLKTAYQLKHAKGG
                     RKPKLSLEDLLMATLQYVREYRTYEQIAAVFGIHESNLIRRSQWVEVTLVQSGVTISR
                     TPLSSEDTVMIDATEVKINRPK"
     gene            complement(465..623)
                     /locus_tag="VVL45_002145"
     CDS             complement(465..623)
                     /locus_tag="VVL45_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HEU4188251.1"
                     /translation="MSLLETAKRHQLNSEKYLSYLLECLPNEETLVNKEVLEAYLPWT
                     KVVQEKCK"
     gene            complement(694..1123)
                     /locus_tag="VVL45_002146"
                     /pseudo
     CDS             complement(694..1123)
                     /locus_tag="VVL45_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295150.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          361 a          286 c          236 g          402 t
ORIGIN      
        1 tttagggcga ttgattttta cttccgtcgc atcaatcatt accgtgtcct cagaactgag
       61 aggagttctt gaaatcgtaa caccactttg aacaagagtt acttcaaccc attggctccg
      121 acggattaag ttgctttcgt gaataccaaa aacagccgca atttgttcat aagttcgata
      181 ttctcgcaca tattgaagag tggccataag aaggtcttct agacttaatt taggttttcg
      241 tccacctttt gcgtgtttaa gttgataagc tgtttttaat acagctaaca tctcttcaaa
      301 agtcgtgcgt tgaacaccaa caagacgctt aaatcgtgca tcggttagtt gtttacttgc
      361 ttcatcattc atagaactat tataccatat tttgtttcgc aggaagtcta ttgaaaaatc
      421 tccagactgg agaactcacg gatagttcct aatctggaga tttcttattt gcacttttct
      481 tgtacaactt tagtccatgg taaataagcc tctaaaacct ctttgtttac gagagtttcc
      541 tcgtttggaa gacattctag aagataggat agatatttct cgctatttaa ttgatgacgt
      601 ttagctgttt ccaacaaact cataataata gctcttgctt tagctccttc aaaactttga
      661 gaaaataacc accctcgctt ttttaaactg agcttaggct aaaagagtcc actggactct
      721 tttactccgt cccataacca atgatttaat ggcgcgttca gctagattat tggaaaggac
      781 cagatgtccg tctttcaaaa tggtcttaaa ggtttcttca tacttgaggc tgtattcaat
      841 tgcccttcct agttttgaac ctgctaaaac tgactgacgc cggcactaag caaagaagtc
      901 ttccattaag ggctggagat gttcttgacg tttctgtagt cgttcatcag ctggcaaagc
      961 ctcccagtct ctttccaagg aaaataactg atcacaataa gctaaacctt tagctcctaa
     1021 ggatgattta tccgcttgct tgggggggcg cttcaaaaaa cttccttctc acatgcgccc
     1081 aacatccaac aagttcagct tcttccagtt gacgataagc agacgagcgg ttcgaagttt
     1141 accaagctta gcgtcgcctt gctcctaaac cttggactgc tatcgcatag gcagaactag
     1201 aggactaaag tcctaagtta ctgccgcaaa atatcacaat gcacatagcc agaataatct
     1261 cctaggaatt cttgtactac tgaac
//
LOCUS       DAVHEX010000127         1271 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVHEX010000127 DAVHEX010000000
VERSION     DAVHEX010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.128"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(1..445)
                     /locus_tag="VVL45_002147"
                     /pseudo
     CDS             complement(1..445)
                     /locus_tag="VVL45_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897432.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(521..748)
                     /locus_tag="VVL45_002148"
     CDS             complement(521..748)
                     /locus_tag="VVL45_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU4188252.1"
                     /translation="MRERLLTQPLLHADETSYRVLESHSHLTYYWTFLSGKVENQAIT
                     LYHHDQSRSGSVVQEFLGDYSGYVHCDMLRQ"
     gene            776..961
                     /locus_tag="VVL45_002149"
                     /pseudo
     CDS             776..961
                     /locus_tag="VVL45_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1056..1271
                     /locus_tag="VVL45_002150"
                     /pseudo
     CDS             1056..1271
                     /locus_tag="VVL45_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433508.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          390 a          280 c          235 g          366 t
ORIGIN      
        1 aggctaaaag agtccactgg actcttttac tccgtcccat aaccaatgat ttaatggcgc
       61 gttcagctag attattggaa aggaccagat gtccgtcttt caaaatagtc ttaaaggttt
      121 cttcatactt gaggctgtat tcaattgccc ttcctagttt tgaacctgct aaaactgact
      181 gacggcggca ccaagcaaag aagtcttcca ttaggggctg gagatgttct tgacgtttct
      241 gtagtcgttc atcagctggc aaagcctccc agtctatttc caaggaaaat aactgatcac
      301 aataagctaa acctttagct cctaaggatg atttatctgc ttgcttgggg gtcgcttcaa
      361 aaaatttcct tctcacatgc gcccaacatc caacaagttc agcttcttcc agttgacgat
      421 aagcttctag cggttcgcag tttaccaagc ttagcgtcgc cttgctccta aaccttggac
      481 tgctatcgca taggcagaac tagaggacta aagtcctaag ttactgccgc aacatatcac
      541 aatgaacata gccagaataa tctcctagga attcttgtac taccgaacca ctccgactct
      601 gatcatgatg gtacagcgtg attgcttgat tctcaacttt cccagacaaa aaggtccaat
      661 agtaggtcag atggctatga ctctctagaa cccgataaga agtttcatcc gcatgaagta
      721 agggctgagt caatagtctc tctcgcaaga ggttataaag gggctttagg aataggagct
      781 ttcacaagct tatccagatg attatctttt actcgttatg gacaatgcta tatggcataa
      841 atcaagtacc ttaaagattc cgactaatat tggttttgca tttattcctc catacacacc
      901 agagatgaac cccattgaac aagtgtggaa agagattcgt aaacgtggat ttatagtaaa
      961 atgaaataat aacaggacaa atcattcagg acagtcaaat cgatttctaa caatgtttta
     1021 gaagcagagg tgtactattc tagtttcaat ctactataga taaacttttg agctattcag
     1081 aagacttgaa acaccactat aatctctatc aactcttgct ttttcacttt cagaataagg
     1141 agcctgacaa atttttcgga ctcattgagg acaatctgaa tcaagttcat cctctaaaac
     1201 cttcctcaaa gataaagaga agattgtcaa tgcccttcaa ctacactatt ctaatgccaa
     1261 actggaagcg a
//
LOCUS       DAVHEX010000128         1242 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVHEX010000128 DAVHEX010000000
VERSION     DAVHEX010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.129"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            1..906
                     /locus_tag="VVL45_002151"
     CDS             1..906
                     /locus_tag="VVL45_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000995574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU4188253.1"
                     /translation="MKMNNVSVRYDDVIALKDITLQINKGDFIGLLGSNGAGKSTLIN
                     SIVGFQEIYLGEIEYCDKDLIVSSQPFAHLGFTPQTTVIDFYTTVKDNVILGLNLAGK
                     FGKNAEKLCQIALEIVGLADKKNNLVETLSGGQLQRVQIARAIAHNPDFYILDEPTVG
                     LDTESAEKFLMYLKDKSLEGKTIIISSHDINLLEKFCKKILFLQNGSISFFGDMRDFV
                     DNSTIKLNFSMQNRISRYQIEFLENFRFKVHIEDNDSFTIEVPIEEKILDVINEVGKA
                     CEIKNFSTSKLTLQESYLQRIGGEK"
     gene            903..>1242
                     /locus_tag="VVL45_002152"
     CDS             903..>1242
                     /locus_tag="VVL45_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU4188254.1"
                     /translation="MKADQLRHKSDLGLRGLAIIAKNEIIAFFRSKGLIISQFLQPIL
                     YVVFIIIGLNSSIKNIQFNDIKTSYAEYTIIGVIALLIIGQMTQVIYRVTIDKKYGLL
                     ALKLCSGVRPL"
BASE COUNT          448 a          144 c          223 g          427 t
ORIGIN      
        1 ttgaaaatga ataatgtaag tgttcgttat gatgacgtaa tagctttaaa agatataact
       61 ttacaaataa ataagggaga tttcattggc ttattaggtt caaatggtgc aggtaaatct
      121 acgttaatta attctattgt aggttttcaa gagatttatt taggagaaat agagtattgt
      181 gataaagatt tgatagttag ttctcaacct tttgctcatt taggctttac tcctcaaacc
      241 acagtaattg atttttatac tactgtgaag gacaatgtaa tattggggct gaaccttgct
      301 ggaaagtttg ggaaaaatgc tgagaagttg tgtcaaatag ccttagaaat tgttgggtta
      361 gctgataaaa aaaataattt ggtagaaaca ttgtcaggtg gacaactgca acgcgtccag
      421 attgctagag caatagctca taatccagat ttttatattt tagatgaacc taccgttggt
      481 ttagatactg aatctgccga aaaattttta atgtatttaa aagataagag tttggaagga
      541 aaaactatta tcatatcttc acatgacata aatctactcg aaaagttttg taaaaaaata
      601 ctttttttac aaaatggctc catatcattt tttggtgata tgcgtgactt tgtagataat
      661 tcaactatca aattaaattt ttcaatgcag aatagaattt ctagatatca aattgaattt
      721 ttagaaaatt ttagatttaa agttcacatc gaagataatg atagttttac aatagaagtc
      781 cctatagaag aaaagatctt agatgttatc aatgaggtag gaaaagcatg tgaaattaaa
      841 aacttttcaa caagtaaatt aaccttacaa gaaagttatt tgcaaagaat aggaggagaa
      901 aaatgaaggc tgatcaatta aggcacaaat cggacttagg tttaagaggt ctagcgatta
      961 ttgctaaaaa tgagattatt gcttttttta gaagtaaagg tttaattatt tctcagtttc
     1021 tacaaccaat cttatatgtt gtttttataa taataggatt aaattcttcg ataaagaaca
     1081 ttcagtttaa tgatataaaa acctcttatg cagaatatac aatcattggt gttatagctt
     1141 tattgataat cgggcagatg actcaagtta tttatagggt gacaatagat aaaaaatatg
     1201 ggctacttgc tcttaagtta tgcagtggag ttcgtccttt at
//
LOCUS       DAVHEX010000129         1124 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVHEX010000129 DAVHEX010000000
VERSION     DAVHEX010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.130"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(1..177)
                     /locus_tag="VVL45_002153"
                     /pseudo
     CDS             complement(1..177)
                     /locus_tag="VVL45_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(190..939)
                     /locus_tag="VVL45_002154"
     CDS             complement(190..939)
                     /locus_tag="VVL45_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEU4188255.1"
                     /translation="MSNNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNG
                     NFLYRHIYSELKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGRALE
                     FMRYKVIYYEDFPCVSDSSMVSYMGKTKELKMYQEELDEKHYIDRISSILCYKSQILI
                     IWKSVEKLLNNIKELYLRNGAAYSIRFWIKK"
BASE COUNT          389 a          219 c          119 g          397 t
ORIGIN      
        1 aagttattct cgcaaaaaaa cttcataagc tcttcatttt tagatggaag aatgcccttt
       61 ttttaaatta tttattttta cagaatctat atcatatgca acaactttat atttagatgc
      121 aaatagtaac gcgtaggcca gcccaacatg ccccaaacca attactgcta tattcataaa
      181 actacttcct tatttcttaa tccaaaatct aatagaataa gctgccccat tccttaaata
      241 caactcttta atattgttta aaagtttttc aactgatttc cagattatca aaatctgaga
      301 tttatagcac aatattgatg atattctatc aatataatgt ttttcatcaa gttcctcttg
      361 atacattttt aattctttag tttttcccat ataactaacc atactactat cacttacaca
      421 tgggaagtcc tcataatata ttactttata acgcataaat tcaagcgccc ttccaatact
      481 attcacaaaa acatgatcaa catggtcacc aagtgaaagc ggacaatata cgacacattt
      541 gtcgtctaaa tgcattaaca gctcttttat gatatcattc tttaatgtgt cctcattttt
      601 taattcacta tagatatgac ggtatagaaa attgccattt ctatctttcc tatagagaca
      661 ttcatagtac gataagtgtc taaaatcaca ttgtagacgt tcacaagcta acctgtcttc
      721 tttcttcctt tcttcaatcg gatatttccc aaggttacac aacttatgaa attgcttagc
      781 agagggctgt agctgttggc tcaaagggta accagaaaat atagtaataa caagtacaat
      841 ttctccttct gaagttaatt ttgaaatata atcaccacag gaaaaaattg cgtcatctaa
      901 atgtggagat aaaaagatat acttagtatt gttactcata accattccct ctacaattta
      961 tctaaaaact cactaagtgt ctgattaaat tccacatcat caaaaaaatt caccttattc
     1021 ttaataatga atatttcgtt aaataaacat atatataaat atttcaatat cctttcaata
     1081 tcatcctcta aattctcctc aatattttgt atcagcccat ttac
//
LOCUS       DAVHEX010000130         1007 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVHEX010000130 DAVHEX010000000
VERSION     DAVHEX010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.131"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..92
                     /locus_tag="VVL45_002155"
     CDS             <1..92
                     /locus_tag="VVL45_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEU4188256.1"
                     /translation="TVLPDIKIKLPIDSRGTPDWNYMERYRDR"
     gene            58..372
                     /locus_tag="VVL45_002156"
     CDS             58..372
                     /locus_tag="VVL45_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188257.1"
                     /translation="MGITWKDIEIDKEREGEIDKKSTPIFYGEFKNVRLSKEEYKNLK
                     EKLNSHTDIMINKLSRYMESSGKTYQNHYVTILKWYEEDKDKLRQKGLNKKMNYDVGE
                     SL"
BASE COUNT          419 a          129 c          191 g          268 t
ORIGIN      
        1 gcacagtatt gccagatatc aaaattaaat taccgataga cagtagaggt actccagatt
       61 ggaattacat ggaaagatat agagatagat aaagagagag agggagagat agataaaaag
      121 tccaccccca ttttttatgg agaattcaaa aatgtaaggt taagcaaaga agaatataaa
      181 aatctaaaag aaaaattaaa ctcacacaca gatataatga taaataaact atccagatac
      241 atggaaagca gtggtaagac ctatcaaaac cactatgtga caatcttaaa atggtatgaa
      301 gaagataaag acaaactaag acagaaaggt ttaaataaaa aaatgaatta tgatgtagga
      361 gaatctttat agataaaaag aggtggaaag gcactattaa agactttaag tctaaaaagg
      421 tatcaaagta tgccagagat aaaaaaatat aaatatgaca ctttaaaggc gattagaaaa
      481 ttaaagaaat gctagaagac ttttagatga cagttaccaa gagggtctaa ttttcactgc
      541 tatttatgca gtgggaattg gaccttttta tataagcaaa ttctatttca aaattggtta
      601 ccaagaaaga cttcttttca ctgttaagta taagagggta atttatagaa ttttgttaca
      661 tcaagataaa tattttctga tggtcaaatt ttaaaaagat taataactct cgataaattt
      721 tgaaccttaa caattgaata gaccaagaca agacgttaat tatttttgga gcgtaataac
      781 ctatccgtca agatctttcg gctgaataat tcggcaaaaa aagttctgct aagcaaaaag
      841 aaagtgagcg ataagtaaga gtttgaatat agggagggat accctatccg ctatgaagaa
      901 aatgagggta atgatacttc taaggttgaa gccggctcat ccggaataga ggcggaagtg
      961 agattcctat gttgctaatc caaagcactt gtcaatgtca aaaaact
//
LOCUS       DAVHEX010000131          962 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVHEX010000131 DAVHEX010000000
VERSION     DAVHEX010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.132"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..436
                     /locus_tag="VVL45_002157"
     CDS             <1..436
                     /locus_tag="VVL45_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HEU4188258.1"
                     /translation="KFISKELISYFCDKVYDEIFVEEVVPHVFLPYESDLLLILPATA
                     NVIGKIANGIADDLVTATVLNFNKKIIFCPNMNSTMWDNHIVQRNVSILKELGHIFLF
                     ESKKTYEVGLRKAIDSTCSMLQPQSLVKELIKLENIVLEEGH"
     gene            657..>962
                     /locus_tag="VVL45_002158"
     CDS             657..>962
                     /locus_tag="VVL45_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEU4188259.1"
                     /translation="MINKKIQQVVLESLQNFLNGNFISPCVVYDFGLLETVLDEFKNQ
                     IPVTFNYQLFYAVKANSNEKILEFLVDKIDGVDVASLSELDVAKKFFPPTQISVNGPA
                     "
BASE COUNT          343 a          118 c          174 g          327 t
ORIGIN      
        1 aaaatttata agtaaggaat taattagtta tttttgtgat aaagtttatg atgaaatatt
       61 tgttgaagag gtagttccgc acgtttttct gccatatgaa tctgacttac ttcttatttt
      121 accagctacg gcaaatgtga ttggcaaaat tgctaatggt attgctgatg atttagttac
      181 agcaactgtt ttaaacttta ataaaaaaat aattttttgt cccaatatga actctactat
      241 gtgggacaat cacatagttc aaagaaatgt atcaattcta aaggagttgg gacatatatt
      301 tttatttgag tctaaaaaaa catatgaggt aggattgcgt aaagcaatag attcaacatg
      361 ttcaatgtta caaccacagt cgttagtaaa agaacttatc aaattagaaa atattgtcct
      421 tgaagaggga cattaaaaac tactgagaat attaatgagg ggaaaaaatg gaaaattcat
      481 caatcgatgt agatatgctg ttggaagaat tgacacaaga agcaatggtc gttgttgctg
      541 ttgataagga ctgttaattt aaacttatgg caatatatga aaggttactg gatgttttaa
      601 attatgcagg cagtagcctt ttattatata caaatggata aagtaaggat aatacaatga
      661 ttaataaaaa aatacaacaa gttgttttgg aatcattaca gaattttttg aatgggaact
      721 tcatttcgcc ttgtgtagtc tatgattttg gcttgctgga aactgtactt gatgaattta
      781 aaaatcaaat tcctgtaaca ttcaattacc aactttttta tgccgttaaa gcaaattcaa
      841 atgagaagat acttgaattc ttagtagata aaattgatgg agttgatgtg gcgtcattat
      901 ctgaattaga tgtggctaaa aaatttttcc caccaactca aatttctgtt aatggtcccg
      961 ca
//
LOCUS       DAVHEX010000132          832 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVHEX010000132 DAVHEX010000000
VERSION     DAVHEX010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.133"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(78..266)
                     /gene="pneA2"
                     /locus_tag="VVL45_002159"
     CDS             complement(78..266)
                     /gene="pneA2"
                     /locus_tag="VVL45_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit PneA2"
                     /protein_id="HEU4188260.1"
                     /translation="MKNDFVIGKSLKELSLEEMQLVYGGTDGADPRSTIICSATLSFI
                     ASYLGSAQTRCGKDNKKK"
     gene            complement(273..497)
                     /gene="pneA1"
                     /locus_tag="VVL45_002160"
     CDS             complement(273..497)
                     /gene="pneA1"
                     /locus_tag="VVL45_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit PneA1"
                     /protein_id="HEU4188261.1"
                     /translation="MTNFNSNEKFCGKSLKSLSADEMSLIYGASDGAEPRWTPTPIIL
                     KSAAASSKVCISAAVSGIGGLVSYNNDCLG"
BASE COUNT          271 a          164 c          104 g          293 t
ORIGIN      
        1 aaaaaaaacc tctctcaaaa ttattagtta cgagagagga aaataaagag caacttttat
       61 ttttaaaatt gaatatttta ttttttcttg ttgtctttgc cacaacgagt ctgagctgac
      121 cccaaatatg atgcaataaa acttaatgta gcagaacata taattgttga tctagggtca
      181 gcaccatctg tgccaccata aaccaattgc atttcttcaa gagataattc tttcaaactc
      241 tttccgatta caaaatcatt tttcatttta atttacccca aacaatcatt attgtagcta
      301 actagccccc caataccaga aacagctgca ctaatgcata ctttcgagct agctgcagct
      361 gatttcaaaa taataggagt aggcgtccaa cgtggctctg caccatcaga tgcaccataa
      421 attaaactca tttcatcagc agacaatgat tttaaacttt ttccacaaaa tttttcgttt
      481 gaattaaagt tggtcataag taataactcc tccttataaa aagttaaata agttgctcta
      541 tatttttcgt tttttcagat aaattataat tctctcattc tcgtgcaaaa tatcgggttt
      601 taatccgcct tcccaacatc aagaccattg gacttctttt gcgattgcac tcgaatctcc
      661 aattgtttat tcttctgaat aaactgttgg taaggaattg tattactaaa cataaaaaca
      721 cctagcaatg caaatgtaac taatttttta attccacgtt tttttctcat gcaatgaaac
      781 ccctttcttt ttccttgttt ttattctaac agatatattt tatttagtca ag
//
LOCUS       DAVHEX010000133          814 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVHEX010000133 DAVHEX010000000
VERSION     DAVHEX010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.134"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(72..>814)
                     /locus_tag="VVL45_002161"
     CDS             complement(72..>814)
                     /locus_tag="VVL45_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEU4188262.1"
                     /translation="LEHLQQFSPKESVGIRVTEPDELNNRMSRFGINICSDNWTSNLQ
                     NPLITRLHFHFGEKDDKFIVKLDKILFKLQEINKLREVREINLGGGFMKLFMENRLKE
                     FFLSLMEIYKKYDIDSTVTTIIEPGSAITSFSAYMITSPVNVSEVNEQQVITLDTSIY
                     TNTLWFVPHIITTLNSSSKERYSTILYGNTCYEHDKYKMKVSLPRLTQNSSIVFFPVG
                     AYIKSNHSNLHRNDFMREVYLWTKNLTY"
BASE COUNT          272 a          138 c           98 g          306 t
ORIGIN      
        1 tttcaaaaat tctttatatt gattatccag aatttgagta gttttatttc ctaaatattc
       61 cttaacttta tctaatatgt caagtttttt gtccacaaat atacctcccg cataaaatca
      121 ttacgatgta aatttgaatg attgcttttt atataagctc ctacaggaaa aaacactata
      181 ctgctatttt gagttaacct tggaagcgaa actttcattt tatacttgtc atgttcataa
      241 caggtattac catagagaat agtactataa cgctctttac tacttgaatt taacgttgta
      301 ataatatgcg gaacaaacca taatgtattg gtgtatattg atgtgtctaa cgtgataacc
      361 tgctgctcat tcacctcact aacattaact gggctagtaa tcatataggc agaaaatgaa
      421 gtaattgcac tacctggttc tattattgta gtcacagtac tatcaatatc gtacttttta
      481 tagatttcca taagtgatag aaaaaattct ttcaaacgat tttccataaa taatttcata
      541 aaaccgcctc caagatttat ttctctaacc tctctaagtt tattaatttc ttgtaactta
      601 aataatattt tatctaactt aacaataaat ttatcatctt tttctccaaa atgaaaatgc
      661 agtcgtgtaa ttaaaggatt ttgtaaatta ctagtccaat tatcactgca aatatttatt
      721 ccaaatctac tcatacgatt attaagttca tctggctccg ttactcttat tccaacagat
      781 tcttttgggg aaaattgttg aagatgttcc aaaa
//
LOCUS       DAVHEX010000134          795 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVHEX010000134 DAVHEX010000000
VERSION     DAVHEX010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.135"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..>795
                     /locus_tag="VVL45_002162"
     CDS             <1..>795
                     /locus_tag="VVL45_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEU4188263.1"
                     /translation="TDYDEKIGSFNTYEIEQTVSKILRVKPNGKIILKSTVPFGFSNK
                     LKRLFDTKNIIFVPEFLREGCSIYDNLYPSRIVVGDETVEGRKIAELFLSISTHSTAN
                     IKNVMLVSPTEAEAIKLFSNTFLALRVAFFNELDSFAERRSLNAEVVIKGVCLDPRIG
                     NFYNNPSFGFGGYCLPKDTKQLKKEFIEINAPVIEAIDISNTNRKQFIVKQILERKPK
                     IVGIYKLGMKYNSDNYKESAILNIINELLIVGIKILVYEPNLNVSI"
BASE COUNT          298 a           92 c          141 g          264 t
ORIGIN      
        1 gacagattat gatgagaaaa ttggtagttt taatacatat gaaatcgaac aaacggtatc
       61 gaagattctg agggtaaaac ctaatggaaa gattatttta aagtcaacag ttccgttcgg
      121 cttttcaaac aaattaaaaa ggctgtttga tacaaaaaat atcatttttg tccctgaatt
      181 tttgagagaa ggttgttcta tatatgataa tttatatcca agtcgcatag ttgttggaga
      241 tgagacagtt gaaggaagaa aaattgcaga gttgttcctt tcgattagta ctcatagtac
      301 tgccaatatt aaaaatgtta tgttagtttc tcctactgaa gcagaagcaa ttaagctttt
      361 ttctaacaca ttcttagctc tccgtgttgc tttttttaat gaactagatt cttttgctga
      421 gaggagaagt ttaaatgctg aagttgtaat aaaaggtgtt tgtttagatc caagaattgg
      481 aaatttttat aataatcctt cttttggatt tggaggatat tgtcttccca aagatactaa
      541 acaattaaaa aaagaattta tagaaataaa tgccccagtg atagaagcga ttgatatttc
      601 aaacacaaat agaaaacagt tcatagttaa acaaatattg gaacgaaagc caaagatagt
      661 aggaatatat aaattgggga tgaaatataa ttcagataat tacaaagagt cagctatttt
      721 aaatataatt aatgaactat tgattgtcgg tataaaaatt ttagtatatg aaccaaattt
      781 aaacgttagt ataga
//
LOCUS       DAVHEX010000135          783 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVHEX010000135 DAVHEX010000000
VERSION     DAVHEX010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.136"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            81..560
                     /locus_tag="VVL45_002163"
     CDS             81..560
                     /locus_tag="VVL45_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU4188264.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWEQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
     gene            654..725
                     /locus_tag="VVL45_002164"
     tRNA            654..725
                     /locus_tag="VVL45_002164"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:687..689,aa:Glu,seq:ttc)
BASE COUNT          295 a          106 c          183 g          199 t
ORIGIN      
        1 gagctaaagg gaacagaatg gagaaaatgg aacaaatgtg ttttctaatc tgttagactg
       61 tatctagaaa ggggaaaatt atgattaaag aattgtatga agaagtccaa gggactgtgt
      121 ataagtgtag aaatgaatat taccttcatt tatgggaatt gtcggattgg gagcaagaag
      181 gcatgctctg cttacatgaa ttgattagta gagaagaagg actggtagac gatattccac
      241 gtttaaggaa atatttcaag accaagtttc gaaatcgaat tttagactat atccgtaaac
      301 aggaaagtca gaagcgtaga tacgataaag aaccctatga agaagtgggt gagatcagtc
      361 atcgtataag tgaggggggt ctctggctag atgattatta tctctttcat gaaacactaa
      421 gagattatag aaacaaacaa agtaaagaga aacaagaaga actagaacgc gtcttaagca
      481 atgaacgatt tcgagggcgt caaagagtat taagagactt acgcattgtg tttaaggagt
      541 ttactatccg tacccactag taagtcatgc aaaaaaaatg aaaaaaatta gaaaaagtag
      601 ttgacaaagt ttgaaaaggc tgtataatag taagagttga aaataacaac tcaggtccgt
      661 tggtcaaggg gttaagacac cgccttttca cggcggtaac acgggttcga atcccgtacg
      721 gactatggta tgttgcgtca ggaccacttg atgaaaaaaa gtttaaaaaa acttaaaaat
      781 ctt
//
LOCUS       DAVHEX010000136          744 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVHEX010000136 DAVHEX010000000
VERSION     DAVHEX010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.137"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            66..744
                     /locus_tag="VVL45_002165"
                     /pseudo
     CDS             66..744
                     /locus_tag="VVL45_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          228 a          135 c          160 g          221 t
ORIGIN      
        1 caggacaaat cgatttctaa caatgtttta gaagtagagg tgtactattc tagtttcaat
       61 ctactatatt tcgtctgatg ggcaaatctt ataaagagat tatagagctt ttagactgta
      121 atcaaacaac gatttggcga aacgtaaaaa aatatgagga gttcggactc gactctctcc
      181 ttcaagaaac acgtggtggt cgtaaccatg catatatgac agttgaggaa gagaaagcct
      241 ttcttgcccg ccatttgaag gctacagagg caggagaatt tgttacaatt gatgccttat
      301 ttcaggctta taaaaaggag ttaggtcgtt cctacacacg tgatgccttc tatcaactgt
      361 tgaagcgcca tggttggcga aatattacgc cacgtccaga acatcctaag aaagcagacg
      421 ctcaaaccat tgttgcgtct aaaaataaaa tctcaatcca agaaggcaag aaagcgtttt
      481 aaatatagta gacgttttcg taaggtttgc ttgatgtacc aagctgaagc tggtttcggt
      541 agaatcagta aactgggatc ttgttgggct ccaataggag taggtccaca tatccatagt
      601 cactatatac gagaatttcg ctattgttat ggagctgttg atgcctatac aggcgaatca
      661 tttttcttaa tagctggtag atgtaatact gagtggatga acgccttttt agaagagctt
      721 tcacaagctt atccttttac tcgt
//
LOCUS       DAVHEX010000137          720 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVHEX010000137 DAVHEX010000000
VERSION     DAVHEX010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.139"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(<1..326)
                     /locus_tag="VVL45_002166"
     CDS             complement(<1..326)
                     /locus_tag="VVL45_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU4188265.1"
                     /translation="MEQLHFITKLLDIKDPNIQILDIINKDTHKEIIAKLDYDAPSCP
                     ECGNQLKKYDFQKPSKIPYLETTGMPSRILLRKRRFKCYHCSKMMVAETPLVKKNHQI
                     PRIINQ"
     gene            451..693
                     /locus_tag="VVL45_002167"
                     /pseudo
     CDS             451..693
                     /locus_tag="VVL45_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800706.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rgg/GadR/MutR family transcriptional regulator"
BASE COUNT          239 a           96 c          132 g          253 t
ORIGIN      
        1 ttttggttga tgatacgagg gatttggtga tttttcttta ccaggggagt ctcagcaacc
       61 atcatttttg aacagtgata gcacttgaaa cgacgctttc taaggagaat tctagaaggc
      121 ataccagtcg tttcaagata aggaatttta gaaggttttt gaaagtcata tttcttcaat
      181 tggtttccgc actcagggca agatggggcg tcgtagtcca gtttggcgat gatttccttg
      241 tgtgtatcct tattgatgat gtctaaaatc tggatattag ggtctttaat atcgagcagt
      301 tttgtgataa aatgtaattg ttccatatga atctttctaa tgagttgttt tgtcgctttt
      361 cattataggt catatgggac tttttttcta caacaaaata ggctccataa tatctataag
      421 ggatttaccc actacaaata ttatagagcc gaaaattcac atctaatata tgcagactac
      481 tttgaaatga aattaaaaaa attattaaag gatgacacaa aagtttttga aaaatctaca
      541 ttcaaatttg tagaaggata taaaatatac ctgacagaat ctaaagaatc tggaattaaa
      601 caaatggaca atgtcataaa atattttgag tttattgaat ctaaaagtat tgctttatat
      661 tttcaaaaac gattaaatga gctgatagat taaatagcat tttctctgtt gagatattgt
//
LOCUS       DAVHEX010000138          702 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVHEX010000138 DAVHEX010000000
VERSION     DAVHEX010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.140"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            1..143
                     /locus_tag="VVL45_002168"
                     /pseudo
     CDS             1..143
                     /locus_tag="VVL45_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(298..621)
                     /gene="tnpB"
                     /locus_tag="VVL45_002169"
     CDS             complement(298..621)
                     /gene="tnpB"
                     /locus_tag="VVL45_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEU4188266.1"
                     /translation="MTIHLSSLGEVYLVCGKTDMRQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDRFKVLYWDGQGFWLLYKRFENGRLIWLSTEKDVKALTPEQVDWLMKGFS
                     ITPKI"
BASE COUNT          241 a          130 c          134 g          197 t
ORIGIN      
        1 aacgtggatt taagaataaa gccttttaaa ctttggaaga tgtcatgaat caactccaag
       61 atgttataca aggactggag aaggaggtga taaagtccat cgttaatcgg agatggacta
      121 gaatgctttt tgaaagcaga tgagtataaa aagaaagtcc tcatttcaat agaaatcacg
      181 actttctgat ggatttatag taaaatgaaa taagaacagg acaaatcgat caggacagta
      241 aaatcgattt ctaacaatgt tttagaagca gaggtgtact attctagttt caatctacta
      301 tatttttgga gtgatagaaa agcccttcat aagccagtct acttgttctg gtgtgagagc
      361 tttgacatcc ttttctgtac ttagccaaat caatctgccg ttctcaaagc gtttatatag
      421 tagccaaaat ccttgaccat cccagtaaag gactttaaag cggtctttac gtccaccaca
      481 aaagagaaag acttgaccgg agaaaggatc caattcaaag tgggttttaa ccagataagc
      541 cagtgaatcg attccttgtc tcatatcagt tttcccacac acgagataga cctcccctag
      601 gctagataga tgaattgtca tagagcaaca ccttatccaa aagctgttct accatttcga
      661 gattgagaga ttgaaaaaaa cttacttcaa gcttgccaat ac
//
LOCUS       DAVHEX010000139          699 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.141, whole genome shotgun sequence.
ACCESSION   DAVHEX010000139 DAVHEX010000000
VERSION     DAVHEX010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.141"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(<1..>699)
                     /locus_tag="VVL45_002170"
     CDS             complement(<1..>699)
                     /locus_tag="VVL45_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase S74"
                     /protein_id="HEU4188267.1"
                     /translation="LSRTKNELSGVKSAQATYEETTTRRLSELTNLANGKASKSELTQ
                     TAEELASRIASVQAGSSRNYFRNSRSRTFTTGGQAVYDYRTFIVPDFWKNSDRFKRDY
                     VRISFDVTFPVALVNDMPAMVHFSAHPWYAYRNLIFKGGTVERQHFEFTIDLSSSSED
                     YQTNNVFIRFGTNYGFPAGLQVVIENAMLSVGNYFPAYQPAYEDQEDRVSVVESNFKQ
                     RADSLDAGVSRLTEG"
BASE COUNT          196 a          171 c          145 g          187 t
ORIGIN      
        1 ccttcagtca gacggcttac accagcgtcg agtgaatcag cacgctgctt aaagttggat
       61 tcgactactg agacacggtc ttcttggtct tcatacgctg gttgataggc tggaaaataa
      121 ttaccaaccg ataacatagc gttctcaatg acgacctgca gaccagcagg aaatccataa
      181 ttagtaccaa aacgaatgaa cacattatta gtctgatagt cctcagaaga actagacaag
      241 tcaatcgtaa actcaaaatg ttggcgttcg acagttccac ctttaaaaat taagtttctg
      301 taggcatacc atggatgagc actaaaatgc accatagcag gcatgtcatt tactagggcg
      361 acagggaaag tcacatcaaa agatatgcga acataatcac gcttgaacct gtcactgttc
      421 ttccagaaat caggaactat gaatgttcga tagtcgtata ccgcttgacc tcctgttgtg
      481 aacgttcttg aacgtgaatt cctgaagtaa ttccgtgaac tacctgcctg cacactcgca
      541 atccgactag ccagctcctc agctgtttgc gtgagttctg acttgctggc tttaccattg
      601 gccaagttgg tcagttctga cagtctacga gtcgtcgtct cctcatacgt cgcttgcgct
      661 gacttcacgc cagacagttc attcttagtc cgactaagt
//
LOCUS       DAVHEX010000140          602 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.143, whole genome shotgun sequence.
ACCESSION   DAVHEX010000140 DAVHEX010000000
VERSION     DAVHEX010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.143"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..>602
                     /gene="lytA"
                     /locus_tag="VVL45_002171"
     CDS             <1..>602
                     /gene="lytA"
                     /locus_tag="VVL45_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEU4188268.1"
                     /translation="SHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKE
                     EFMTDYRLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYL
                     AKWGISREQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSS
                     GYMLADRWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFN"
BASE COUNT          180 a          127 c          155 g          140 t
ORIGIN      
        1 ctcgcacatt gttgggaacg gttgcatcat gcaggtagga cctgttgata atggtgcctg
       61 ggacgttggg ggcggttgga atgctgagac ctatgcagcg gttgaactga ttgaaagcca
      121 ttcaaccaaa gaagagttca tgacggacta ccgcctttat atcgaactct tacgcaatct
      181 agcagatgaa gcaggtttgc cgaaaacgct tgatacaggg agtttagctg gaattaaaac
      241 gcacgagtat tgcacgaata accaaccaaa caaccactca gaccacgttg acccttatcc
      301 atatcttgct aaatggggca ttagccgtga gcagtttaag catgatattg agaacggctt
      361 gacgattgaa acaggctggc agaagaatga cactggctac tggtacgtac attcagacgg
      421 ctcttatcca aaagacaagt ttgagaaaat caatggcact tggtactact ttgacagttc
      481 aggctatatg cttgcagacc gctggaggaa gcacacagac ggcaactggt actggttcga
      541 caactcaggc gaaatggcta caggctggaa gaaaatcgct gataagtggt actatttcaa
      601 cg
//
LOCUS       DAVHEX010000141          591 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.144, whole genome shotgun sequence.
ACCESSION   DAVHEX010000141 DAVHEX010000000
VERSION     DAVHEX010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.144"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(84..365)
                     /locus_tag="VVL45_002172"
     CDS             complement(84..365)
                     /locus_tag="VVL45_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188269.1"
                     /translation="MWLIILWNAKPDTPLFNFKDEVIKYKTYEPFESSIKRVNTTIKN
                     GSKGKTLTEMINGYRADNDIRDEICNFNILKNKIRDMKNQQGNTMESYF"
BASE COUNT          184 a          114 c           64 g          229 t
ORIGIN      
        1 actgatattt taactatatt gtatagtcta ctatttaaat ctcaattagg atattttata
       61 gaatacccaa aattcaacaa taactaaaag taagattcca ttgtatttcc ttgttggttt
      121 ttcatatcac gaattttatt tttcagaata ttaaagttac aaatttcatc tctaatatcg
      181 ttatccgctc tgtagccatt aatcatctca gtcagagttt tccctttact accattcttt
      241 atagtagtat ttactctttt tatactgctt tcaaaaggct catatgtttt atacttaatt
      301 acttcgtctt taaaattaaa taaaggtgta tccggcttag cattccataa aataatcaac
      361 cacattatag cattataaag ctgctttgca gtttcattcc acttttcagg ttataaattt
      421 ccactccctg tattgtcata ttcttgtcta ttatttacat gagaatcacc tacaagcgga
      481 ttaaaaagag gtagcccttt gtgattaatt agttctaaaa aatcccttgc cgcacccatg
      541 caacactgaa taacttcctt cttgccatca attttactct cagcgataaa t
//
LOCUS       DAVHEX010000142          568 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.146, whole genome shotgun sequence.
ACCESSION   DAVHEX010000142 DAVHEX010000000
VERSION     DAVHEX010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.146"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            1..568
                     /locus_tag="VVL45_002173"
                     /pseudo
     CDS             1..568
                     /locus_tag="VVL45_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          195 a          112 c          120 g          141 t
ORIGIN      
        1 aagggaaatc aaatagagaa tttgcctctc tacttggaaa agctcctcaa actatccaca
       61 ctgaaatcaa gcgtaggaca gtccgacaat gtcttggaaa agggcgcttc aaagaggttt
      121 attctgccga ctatgctcaa cagtcttatg aaaacaatcg caagcgcttg gtcaagaaat
      181 caagcttgac caaggaacta aaggaaaaga ttctccacta tcataaccaa aaatttttgc
      241 ctgaaatgat ggttatggct aaaggggtta acgtgggaat ttcaaccatt tactattgga
      301 ttcatcatgg aaaattgggg taaagcaagc aggatctgct ttaccctaga aaaggaaaag
      361 cgcttaagaa acaggccagc accaacttta aacctgctgg tcaatccatc gaacagcggc
      421 ttgaagctat caattttcgc ttggagaatg ggcattatga gattgataca gttctactta
      481 cgagagcgaa aaactactgc ttgcttgtct tgacggatcg aaaaagtaga catcagatca
      541 tccgattgat tccaaataaa agtgctga
//
LOCUS       DAVHEX010000143          568 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.147, whole genome shotgun sequence.
ACCESSION   DAVHEX010000143 DAVHEX010000000
VERSION     DAVHEX010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.147"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            <1..>568
                     /locus_tag="VVL45_002174"
     CDS             <1..>568
                     /locus_tag="VVL45_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEU4188270.1"
                     /translation="PIRHPERLGKPNAQITYTDDEIQVAKLAGKYTTEDGYIFDPRDI
                     TSDEGDAYVTPHMTHSHWIKKDSLSEAERAAAQAYAKEKGLTPPSTDHQDSGNTEAKG
                     AEAIYNRVKAAKKVPLDRMPYNLQYTVEVKNGSLIIPHYDHYHNIKFEWFDEGLYEAP
                     KGYTLEDLLATVKYYVEHPNERPHSDNGF"
BASE COUNT          183 a          119 c          126 g          140 t
ORIGIN      
        1 ccgattcgtc atccagaacg tttaggaaaa ccaaatgcgc aaattaccta cactgatgat
       61 gagattcaag tagccaagtt ggcaggcaag tacacaacag aagacggtta tatctttgat
      121 cctcgtgata taaccagtga tgagggggat gcctatgtaa ctccacatat gacccatagc
      181 cactggatta aaaaagatag tttgtctgaa gctgagagag cggcagccca ggcttatgct
      241 aaagagaaag gtttgacccc tccttcgaca gaccatcagg attcaggaaa tactgaggca
      301 aaaggagcag aagctatcta caaccgcgtg aaagcagcta agaaggtgcc acttgatcgt
      361 atgccttaca atcttcaata tactgtagaa gtcaaaaacg gtagtttaat catacctcat
      421 tatgaccatt accataacat caaatttgag tggtttgacg aaggccttta tgaggcacct
      481 aaggggtata ctcttgagga tcttttggcg actgtcaagt actatgtcga acatccaaac
      541 gaacgtccgc attcagataa tggttttg
//
LOCUS       DAVHEX010000144          558 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.denovo.148, whole genome shotgun sequence.
ACCESSION   DAVHEX010000144 DAVHEX010000000
VERSION     DAVHEX010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.denovo.148"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            complement(<1..>558)
                     /locus_tag="VVL45_002175"
     CDS             complement(<1..>558)
                     /locus_tag="VVL45_002175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188271.1"
                     /translation="VDAEAEALVEAEADALVLAEAEALVLAEADALVDAEAEALVLAE
                     AEALVLAEAEALVEADSDAEVLAEADALVEAEADALVDAEAEALVDAEAEALVEAEAD
                     ALVLAEAEALVLAEAEALVLAETEALVDAEAEALVDAEADALVLAETEALVLAEAEAL
                     VLAEADALVLAEAEALVDADSDAEVL"
BASE COUNT          131 a          183 c          133 g          111 t
ORIGIN      
        1 aagtacctca gcgtctgaat cggcatcaac aagtgcttcg gcttcagcaa gcacaagcgc
       61 gtcagcttca gcaagcacaa gcgcctcagc ttcagcaagc acaagtgctt cagtctcagc
      121 aagtaccagt gcgtcagctt cagcgtcaac gagtgcctcg gcttcagcgt caacgagtgc
      181 ctcagtctca gcaagcacaa gcgcctcggc ttccgcaagt accagtgctt cggcctcagc
      241 aagtaccagt gcgtcagctt cagcctcaac gagtgcttcg gcttccgcat caacaagcgc
      301 ctcagcttca gcgtcaacaa gtgcgtcagc ttcagcctca acgagtgcgt cggcttcagc
      361 aagtacctca gcgtctgaat cggcctcaac gagtgcttcg gcttcagcaa gtacaagtgc
      421 ttcggcctca gcaagtacta gcgcctcagc ctcagcatca acaagtgcgt cagcctcagc
      481 aagcaccagt gcttcggctt cagcaagtac aagtgcgtca gcttcagcct caacgagtgc
      541 ttcggcttcc gcatcaac
//
LOCUS       DAVHEX010000145        10633 bp    DNA     linear   BCT 17-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate SC22-0384-P
            SAMN37284184-rid19589573.guided.068, whole genome shotgun sequence.
ACCESSION   DAVHEX010000145 DAVHEX010000000
VERSION     DAVHEX010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37284184
            Sequence Read Archive: SRR25917636
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001913176.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001913176.1
            Genome Coverage       :: 105x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/04/2023 19:06:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,140
            Genes (coding)                    :: 2,019
            CDSs (with protein)               :: 2,019
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 62 of 121
            Pseudo Genes (incomplete)         :: 56 of 121
            Pseudo Genes (internal stop)      :: 32 of 121
            Pseudo Genes (multiple problems)  :: 25 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10633
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37284184-rid19589573.guided.068"
                     /serotype="19A"
                     /isolate="SC22-0384-P"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Canada: Quebec"
                     /collection_date="2021"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
     gene            67..1941
                     /locus_tag="VVL45_002176"
     CDS             67..1941
                     /locus_tag="VVL45_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEU4188272.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDILSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSDLELVGILDKDAKKKGQKLGGIPVLGSYDDLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVAKAVDEAKVPKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLEAIDQKIEEFRTLSGDELKQAIIAFANQTTHVEAVSLLVKE
                     "
     gene            2456..2626
                     /locus_tag="VVL45_002177"
     CDS             2456..2626
                     /locus_tag="VVL45_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HEU4188273.1"
                     /translation="MKYFEVELENPDEFLKLQTEDFVKANRLLLRKIIQSVTVYEENF
                     VISFKSGIELEV"
     gene            3012..4229
                     /gene="mef(A)"
                     /locus_tag="VVL45_002178"
     CDS             3012..4229
                     /gene="mef(A)"
                     /locus_tag="VVL45_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(A)"
                     /protein_id="HEU4188274.1"
                     /translation="MEKYNNWKRKFYAIWAGQAVSLITSAILQMAIIFYLTEKTGSAM
                     VLSMASLVGFLPYAILGPAIGVLVDRHDRKKIMIGADLIIAAAGAVLAIVAFCMELPV
                     WMIMIVLFIRSIGTAFHTPALNAVTPLLVPEEQLTKCAGYSQSLQSISYIVSPAVAAL
                     LYSVWDLNAIIAIDVLGAVIASITVAIVRIPKLGNQVQSLEPNFIREMKEGVVVLRQN
                     KGLFALLLLGTLYTFVYMPINALFPLISMEHFNGTPVHISITEISFAFGMLAGGLLLG
                     RLGGFEKHVLLITSSFFIMGTSLAVSGILPPNGFVIFVVCCAIMGLSVPFYSGVQTAL
                     FQEKIKPEYLGRVFSLIGSIMSLAMPIGLILSGFFADKIGVNHWFLLSGILIIGIAIV
                     CQMITEVRKLDLK"
     gene            4349..5812
                     /gene="msr(D)"
                     /locus_tag="VVL45_002179"
     CDS             4349..5812
                     /gene="msr(D)"
                     /locus_tag="VVL45_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(D)"
                     /protein_id="HEU4188275.1"
                     /translation="MELILKAKDIRVEFKGRDVLDINELEVYDYDRIGLVGANGAGKS
                     TLLRVLLGELTPPGCKMNRLGELAYIPQLDEVTLQEEKDFALVGKLGVEQLNIQTMSG
                     GEETRLKIAQALSAQVHGILADEPTSHLDREGIDFLIGQLKYFTGALLVISHDRYFLD
                     EIVDKIWELKDGKITEYWGNYSDYLRQKEEERKSQAAEYEQFIAERARLERAAEEKRK
                     QARKIEQKAKGSSKKKSTEDGGRLAHQKSIGSKEKKMYNAAKTLEHRIAALGKVEAPE
                     GIRRIRFRQSKALELHNPYPIVGAEINKVFGDKALFENASFQIPLGAKVALTGGNGIG
                     KTTLIQMILNHEEGISISPKAKIGYFAQNGYKYNSNQNVMEFMQKDCDYNISEIRSVL
                     ASMGFKQNDIGKSLSVLSGGEIIKLLLAKMLMGRYNILIMDEPSNFLDIPSLEALEIL
                     MKEYTGTIVFITHDKRLLENVADVVYEIRDKKINLKH"
     gene            complement(5930..6229)
                     /locus_tag="VVL45_002180"
     CDS             complement(5930..6229)
                     /locus_tag="VVL45_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188276.1"
                     /translation="MNQLEFQRNHLQMDYYSESYQDFERDFYRYSNMNIPLTFLTDDI
                     LKTMATSRKNYFVLNKEKSRDNRDHFFIFEVSTVDENPLIYHYTYKKTTIYLAEK"
     gene            complement(6216..6584)
                     /locus_tag="VVL45_002181"
     CDS             complement(6216..6584)
                     /locus_tag="VVL45_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188277.1"
                     /translation="MIDRSYLPFQSAREYQDTKMQKWMGFFLSEHASALSDDTNKVTY
                     MSDLSLEKKLLLLSQVYAGQLRTRIQVIEKNKRVSYTGTIPSLTKDFILIKTTTGHIN
                     LKLKDIISIELVEEVLYESA"
     gene            complement(6597..6932)
                     /locus_tag="VVL45_002182"
     CDS             complement(6597..6932)
                     /locus_tag="VVL45_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imsC-like protein"
                     /protein_id="HEU4188278.1"
                     /translation="MKQEKNTVQFSEIRSKGCNDIEMLERFLHGIVETATSKLRQRKL
                     KTTEISIRLVHAKSENRLPLEFTFSIKPTSSSVIIYTEVINRFKECYTGGGIQGFTIQ
                     FDKNTLASA"
     gene            complement(6929..7174)
                     /locus_tag="VVL45_002183"
     CDS             complement(6929..7174)
                     /locus_tag="VVL45_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU4188279.1"
                     /translation="MRQIGVSYSGFVDESYTLLSLFDDVEQIEKDNRLQTAIDVVREQ
                     FGFLAIQKGTVLTEGSRNIERSKLIGGHSAGGLEGLK"
     gene            complement(7535..8167)
                     /locus_tag="VVL45_002184"
     CDS             complement(7535..8167)
                     /locus_tag="VVL45_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEU4188280.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTHTFKELGVPSPDAKTIR
                     GFMGPPLENSFATCLPKEQISEAVQIYRSYYKAKGIHEAQLFPHIVDLLEKLSSKYPL
                     YITTTKNTPTAQDMTKNLGIFHFFDGIYGSSPEAPHKADVIRQALHTHQLAPEQAIII
                     GDTKFDMQGAQETGIQKLAVTWGFGEQADLLNYQPDYIAHKPIEVLEYFQ"
     gene            complement(8189..9061)
                     /gene="sdaAA"
                     /locus_tag="VVL45_002185"
     CDS             complement(8189..9061)
                     /gene="sdaAA"
                     /locus_tag="VVL45_002185"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEU4188281.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIKNGKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAA
                     TPTGRRLQKEIFGE"
     gene            complement(9070..9741)
                     /gene="sdaAB"
                     /locus_tag="VVL45_002186"
     CDS             complement(9070..9741)
                     /gene="sdaAB"
                     /locus_tag="VVL45_002186"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEU4188282.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            9983..10555
                     /locus_tag="VVL45_002187"
     CDS             9983..10555
                     /locus_tag="VVL45_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU4188283.1"
                     /translation="MKSTTKKIKTTLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNGDYSAE
                     NQERVADAYVAGRYGSWTAAKNFWLNNGWY"
BASE COUNT         3218 a         1892 c         2278 g         3245 t
ORIGIN      
        1 ccaaataaat aaaaatatgc tataataaca atatagtaaa actcttttaa ggaggagtaa
       61 atttctatga ataaaaaact cacagattat gtgattgatc tggtggaaat tttaaataaa
      121 caacaaaaac aggttttctg gggaatattt gatattttga gtatggtggt ttccatcatt
      181 gtatcttata tcttattcta cggcttgatt aatccagcac ctgttgacta catcatctat
      241 acgagtttgg ccttcctgtt ctatcaattg atgattggat tttggggatt gaacgctagt
      301 atcagtcgtt acagcaagat tacggatttc atgaaaatct tttttggtgt gactgccagc
      361 agtgtcttgt catatagtat ctgctatgcc ttcttgccac tcttttcaat ccgtttcatc
      421 attctcttta tcttgttgag taccttcttg attttgttgc cacggattac ttggcagtta
      481 atctactcta gacgcaaaaa aggaagtggt gatggagagc accgtcggac tttcttgatt
      541 ggtgctggtg atggtggggc cctctttatg gatagctacc aacatccaac cagtgatttg
      601 gaacttgttg gcattttgga taaggatgct aagaaaaagg gacaaaaact tggtgggatt
      661 cctgttttgg gttcatatga tgatctgccg gaattagcca aacgccacca aatcgagcgt
      721 gttatcgttg cgattccgtc gcttgacccg tcagaatatg aacgtatctt acagatgtgt
      781 aataagctgg gtgtcaaatg ttacaagatg cctaaggttg aaaccgttgt tcagggactt
      841 caccaagcag gtactgggtt ccaaaaaatt gatattacgg accttttggg tcgtcaggaa
      901 attcgtcttg acgaatcgcg tctgggcgca gaactgacag gtaagacaat cttggttaca
      961 ggtgctggag gttcaatcgg ttctgaaatc tgtcgccaag ttagtcgctt caatcctgaa
     1021 cgcatcgtct tgcttggtca tggggaaaac tcaatctacc ttgtttatca tgaattgatt
     1081 cgtaagttcc aagggattga ttatgtacct gtgattgcgg atattcaaga ttatgaccgt
     1141 ctcttgcaag tctttgagca gtacaagcca gctattgttt atcatgcggc tgcccacaag
     1201 cacgttccta tgatggagcg caatccaaaa gaagccttca aaaacaatat tcgtggaact
     1261 tacaatgttg ctaaggctgt tgatgaagcc aaagtaccta agatggttat gatttcgaca
     1321 gataaggcgg ttaatccacc aaatgttatg ggagcaacca agcgcgtggc tgagttgatt
     1381 gtcactggct ttaaccaacg tagccaatcg acctactgtg cggttcgttt tgggaatgtt
     1441 cttggtagcc gtggtagtgt gattccagtc tttgaacgtc agattgctga aggtgggcct
     1501 gtaacggtga cagacttccg catgacccgt tactttatga ctattccaga agctagccgt
     1561 ctggttatcc atgctggtgc ttatgccaag gatggagaag tctttatcct tgatatgggc
     1621 aaaccagtca aaatttatga cttggccaag aaaatggtcc ttctaagtgg acacaccgaa
     1681 agcgaaattc caattgttga agttggaatc cgcccaggcg aaaaacttta cgaagaactc
     1741 ttggtatcaa ccgaacttgt tgacaatcag gttatggata agattttcgt tggtaaggtt
     1801 aatgtcatgc ctctagaagc catcgatcaa aagattgaag agttccgcac tctcagtgga
     1861 gatgagttga agcaagccat tattgccttt gccaatcaaa caacccatgt tgaggcggta
     1921 agtttgctag tcaaggagta aaacgacgaa gattagcatt tacttccgcc catgcgatag
     1981 ctgtccgtga ttgacaagtg ctagcacgca gacagaacgg agatagcgaa ccgctgagtg
     2041 tgtcgctctg ctcgtaaaag cttagaaacc tttgaacgaa agggataatg aaagccttga
     2101 ttgcaaggct ttttgcttta tggtgggtaa gtatcagagt gagaaaattt ttggaatgag
     2161 tagaagtgat agctagaaat tatcagtttc tatttccatt taccctgtgg gtacgtgttt
     2221 gtttccattg acaaggagtt tgtgggaata gaaatgtacc caccttgttt gaatcaagtg
     2281 aagtgtagtt gaaggaaatc tgttgaaagc aatacttcat tttaccgaat aagtaataat
     2341 ttaggcaact tcaaatcgat taaaaaaaac tattttaaag gttaagagta gacaaaaatt
     2401 gtccactctt ttttgcaaac tcaatttatc aataaatgaa atgagggaat gtaaaatgaa
     2461 atattttgag gttgagttag aaaatcctga tgaattttta aaactacaaa cagaagattt
     2521 tgtgaaagct aatcgcttgc tactaaggaa gataatccag agcgttacag tctatgaaga
     2581 aaacttcgtc atatccttta aatctggcat cgaattggaa gtatgagtct cattccataa
     2641 cttttatatt gaacatatca tctttttgtg ttatactata aattgatata aacaaagatg
     2701 taggaggaac cgaaactatg acagcctcaa tgcgtttaag ataagctggc aataaaaaaa
     2761 gcagaatcta tacccgatga taggcttttt tgttgtgctt atttatacga tattgagcat
     2821 tcattagtta cggtgaggat attggttatt taactatacc tttatttaac tatgtcttta
     2881 atatgaatgt ttccaaattg tatgtatgca gaccaaaagc cacattgtgg ggtttggcct
     2941 gcatttttta ttgcctagaa tgctattcaa aatagaaatt caagcaaaat aatatgcagg
     3001 agataatata aatggaaaaa tacaacaatt ggaaacgaaa attttatgca atatgggcag
     3061 ggcaagcagt atcattaatc actagtgcca tcctgcaaat ggcgattatt ttttacctta
     3121 cagaaaaaac aggatctgcg atggtcttgt ctatggcttc attagtaggt tttttaccct
     3181 atgcgatttt gggacctgcc attggtgtgc tagtggatcg tcatgatagg aagaagataa
     3241 tgattggtgc cgatttaatt atcgcagcag ctggtgcagt gcttgctatt gttgcattct
     3301 gtatggagct acctgtctgg atgattatga tagtattgtt tatccgtagc attggaacag
     3361 cttttcatac cccagcactc aatgcggtta caccactttt agtaccagaa gaacagctaa
     3421 cgaaatgcgc aggctatagt cagtctttgc agtctataag ctatattgtt agtccggcag
     3481 ttgcagcact cttatactcc gtttgggatt taaatgctat tattgccatc gacgtattgg
     3541 gtgctgtgat tgcatctatt acggtagcaa ttgtacgtat acctaagctg ggtaatcaag
     3601 tgcaaagttt agaaccaaat ttcataaggg agatgaaaga aggagttgtg gttctgagac
     3661 aaaacaaagg attgtttgcc ttattactct taggaacact atatactttt gtttatatgc
     3721 caatcaatgc actatttcct ttaataagca tggaacactt taatggaacg cctgtgcata
     3781 tttctattac ggaaatttcc tttgcatttg ggatgctagc aggaggctta ttattaggaa
     3841 gattaggggg cttcgaaaag catgtattac taataacaag ttcatttttt ataatgggga
     3901 ccagtttagc cgtttcggga atacttcctc caaatggatt tgtaatattc gtagtttgct
     3961 gtgcaataat ggggctttcg gtgccatttt atagcggtgt gcaaacagct ctttttcagg
     4021 agaaaattaa gcctgaatat ttaggacgtg tattttcttt gatcggaagt atcatgtcac
     4081 ttgctatgcc aattgggtta attctttctg gattctttgc tgataaaatc ggtgtaaatc
     4141 attggttttt actatcaggt attttaatta ttggcattgc tatagtttgc caaatgataa
     4201 ctgaggttag aaaattagat ttaaaataaa caatattgga ggaatattta tgtatcttat
     4261 tttcatgtaa ctcttcctgc taaaatcgca gggttttccc tgcatacaag caaatgaaag
     4321 catgcgatta tagacaggag gaaatgttat ggaattaata ttaaaagcaa aagacattcg
     4381 tgtggaattc aaaggacgcg atgttttaga tataaatgaa ttagaagtat atgattatga
     4441 ccgtattggt ttagtaggag caaatggtgc tggaaaaagc actttactca gggtactttt
     4501 aggagaatta actcccccag gatgtaaaat gaatcgtctg ggtgaacttg cctatattcc
     4561 ccagttggac gaagtaactc tgcaggagga aaaagatttt gcacttgtag gcaagctagg
     4621 tgttgagcaa ttaaatatac agactatgag cggtggtgaa gaaacaaggc ttaaaatagc
     4681 acaggcctta tcggcacagg ttcatggtat tttagcggat gaacctacga gccatttaga
     4741 ccgtgaagga attgattttc taataggaca gctaaaatat tttacaggtg cactgttagt
     4801 tattagccat gaccgctatt ttcttgatga aatagtagat aaaatatggg aactgaaaga
     4861 tggcaaaatc actgagtatt ggggaaacta ttctgattat cttcgtcaga aagaggaaga
     4921 acgtaagagc caagctgcag aatacgaaca atttattgcg gaacgtgccc gattggaaag
     4981 ggctgcggag gaaaagcgaa aacaggctcg taaaatagaa cagaaggcaa aaggttcttc
     5041 aaagaaaaaa agtactgaag acggagggcg tttagctcat caaaaatcaa taggaagtaa
     5101 ggaaaaaaag atgtataatg ctgctaaaac cctagagcac aggattgcgg ccttaggaaa
     5161 agtagaagct ccggaaggca ttcgcagaat tcgtttcagg caaagtaaag cattggagct
     5221 ccataatcca taccctatag tcggtgcaga aattaataaa gtatttgggg ataaggctct
     5281 gtttgaaaat gcatcttttc aaattccgtt aggagcaaaa gtggcgttaa ctggtggtaa
     5341 tggaatcgga aaaacaactt taatccaaat gatcttaaac catgaagaag gaatttctat
     5401 ttcgcctaag gcaaaaatag gttactttgc acagaatggt tacaagtaca acagtaatca
     5461 gaatgttatg gagtttatgc agaaggattg tgactacaat atatcagaaa ttcgttcagt
     5521 gctagcatct atggggttca aacagaacga tattggaaaa agtttatctg ttttaagcgg
     5581 tggagaaatt ataaaattgt tgcttgctaa aatgctcatg ggtagatata acatcctaat
     5641 aatggatgaa cccagtaact tccttgacat accaagttta gaggctttgg aaatactaat
     5701 gaaggagtac accggaacta tcgtgtttat cacccacgat aaacgattac tcgaaaatgt
     5761 agcagatgta gtttatgaaa ttagagataa gaaaataaat ctgaaacatt aaatttaagg
     5821 tagtcgctgg tcagtatagt ctgttctggt tggcgactcc attgttaaag agtataaaga
     5881 ctttagattt tatgaatatt aaaaatagga acagtcaatt gaactgctcc tatttttctg
     5941 ctaaatatat tgtagttttc ttatatgtat aatgatagat tagcggattc tcatctacgg
     6001 tacttacttc aaatatgaag aagtgatcgc ggttatctct ggacttttcc ttattgagga
     6061 caaagtaatt cttacgtgaa gtcgccattg tttttaggat atcatcagtt aggaaggtca
     6121 atggaatatt catgttagag tagcggtaga agtcacgttc aaaatcttgg tagctctcgc
     6181 tataatagtc catttgtagg tgattacgct gaaactcaag ctgattcata gagcacctcc
     6241 tcgacaagtt caatactaat aatgtctttt aatttcaaat tgatgtgacc tgttgtagtt
     6301 tttatcaaaa tgaaatcttt ggtcagactt ggtattgttc cagtgtagga aacacgcttg
     6361 tttttttcaa tcacttgaat gcgtgtgcgt agctgcccgg cgtatacttg actgaggagt
     6421 aataatttct tctctagtga taagtcagac atgtacgtta ctttgtttgt atcatcagag
     6481 agtgctgatg catgttcaga taggaaaaag cccatccatt tttgcatctt tgtatcctgg
     6541 tactctcttg ctgattgaaa tggtaaatat gaacggtcaa tcatatcaaa tcctttctat
     6601 gcagaggcaa gggtattttt atcaaattga atcgtaaaac cttgaattcc cccacctgtg
     6661 taacattctt taaagcgatt gattacctca gtatagatta tcacagatga gcttgttggc
     6721 ttaatgctaa atgtaaattc caatggtaat cggttttcag atttagcatg tactagtcgt
     6781 atcgatattt cagttgtttt gagttttctc tgacgaagtt ttgaagttgc tgtttcaacg
     6841 attccatgta aaaatctttc aagcatttca atatcattac atcctttgct acggatttct
     6901 gaaaattgta ctgtattttt ttcttgtttc attttaatcc ctccaatcca cccgcggaat
     6961 gaccaccgat aagtttactg cgttcaatat ttctggaacc ttcagttagg acggttcctt
     7021 tttgtatggc taaaaaacca aactgttctc tgacaacatc aatagctgtc tgaagtctat
     7081 tatctttttc aatttgttct acatcatcaa agagtgatag tagagtatag ctttcatcta
     7141 cgaagccact ataagataca ccaatttgtc tcactgcacc agaggtatat tttttcggaa
     7201 taatacaagt acatgactca ccattgtttt ggggagattt gcgggttcaa ttttattctg
     7261 agcatttata gattttttca tctcagtcct agaatagcca atatgaatag aaacgacagt
     7321 agtcaatact agggctactg ttccgttcca cagtatcatt taaaaatcat tttcacaccc
     7381 tttcgtctat tagtatagaa gaaagctctc agcacacgtt gaataaaaaa gaaaaacgca
     7441 tattatcagt tctgaagaac cttggtaata tgcgttttgt tatgtagaaa cttgctccat
     7501 tttcttctga aatggggttg tcgcccagtc tatgttattg aaaatactcc aaaacttcta
     7561 tgggtttgtg agcgatataa tcaggctgat agtttagtag atctgcttgt tctccaaatc
     7621 cccaagtgac ggccaatttc tgaatgcctg tttcttgagc cccctgcata tcaaacttgg
     7681 tatctccgat gatgatggct tgttctggtg ctagttgatg tgtatgcaag gcttggcgaa
     7741 tgacatcggc cttatggggt gcttcagggc tggaaccata aatgccatca aagaaatgaa
     7801 agattcccaa gttcttagtc atgtcttgag cggttggagt attctttgta gtggtgatat
     7861 aaagagggta cttgctcgat aacttctcaa gcaagtctac aatatgaggg aagagctgag
     7921 cttcatggat gccttttgcc ttgtagtaag aacggtatat ctgcacagcc tcagaaattt
     7981 gttctttagg caggcaggtc gcaaaactat tttcaagagg tggccccata aaaccacgaa
     8041 tcgttttggc atcagggcta ggcaccccca gctccttaaa ggtatgggta aaggcattgt
     8101 gaatcccgat agaactgtca acgagggttc catccagatc gaaaaaaatc gctgtgatag
     8161 aggtcatggt ttctcctatt tgataaactt attctccgaa aatttctttt tggaggcgac
     8221 gaccagtagg ggtggcagcg agtccacctt cagctgtttc acgaaaggca gtcggcatgc
     8281 ttgctcctac ttggtacatg gcatcgataa cttcatccac aggaatttta gattcgatac
     8341 ctgccaaggc catgtctgct gcgatgaagg caaagctagc tcccatggca ttacgtttaa
     8401 cacagggaac ttcaaccaag ccggcaacag ggtcacagat gaggcctagc atatttttaa
     8461 tgacaaaggc aatggcctga ctggcctgat acggtgttcc acctgcagcc agagtcaagg
     8521 ctgcagcact catagcagag gccgaaccaa cctcggcctg acagccacct tcagcacctg
     8581 agatagaggc attgtttgcg atgactagtc caaaggcacc ggcagcaaag aggaaatcca
     8641 gctgttgctc gtggctgagg tctaattttt caatagcagc tgttagaact gagggtagac
     8701 agccagcact tcctgcggtc ggagtggcac aaaccaagcc cattttggca ttgtgttcat
     8761 tgactgcaat ggcatttcgg gcagcagaga gaatcgtgta atctgataga gtttttccgt
     8821 ttttgatgta atgatccaat ttggcagcat ctccacctgt caggccacta cgagatttat
     8881 tttcattgag gccaagttgg acagaggctt tcatgacttc cagattgcgt tccatgagaa
     8941 ggaagacttc ttcacgttcg cgaccggtca attcaaactc tgttgtaatc atgagttctg
     9001 cgacatttcc ttgaaagtcc agatctgctt gctcgaccaa ttctttgata gaataaaaca
     9061 tgcttcctcc tatttaaaga aattgacatt gtggagatga gggatttttc gaatttcttc
     9121 gatggcctca tcacagttgc gactgtcaac ttcgataatc ataatagctt tttcaccagc
     9181 tttttcacga gtaacattca tctgggcgat attgatacca tagcgggaaa gggcctctgt
     9241 tacgagggca atcatacctg gaatatcttg atgaacaatg atgatagtcg gtgtattcat
     9301 attgagagag acggcaaagc cattgagttc ggttacctga atattccctc ccccgataga
     9361 aatgccagtc acactgatag ttttatgggc atttttaacg gtaattttag tggtattagg
     9421 atgcggagca ttgctgtctt tttgaatagt ccagacaatc ttgataccac gtttgtgggc
     9481 aatctccaga ctgtttggga tttcaggatc atctgtatcc atgcccaaaa tacctgcaac
     9541 aagggctagg tctgtcccgt gaccacgata ggttttggca aatgagttaa aaagttggaa
     9601 ttcgacttct gtcggagtat catcaaaaat ggaagagaca atcttcccaa tacgaacagc
     9661 gccagcggta tggctactag atgggccaat cataactggt ccgatgatat caaagacaga
     9721 ttgaaaacga agtgatttca tcagtttccc cttataaaaa ttcttatctc tattatatca
     9781 aagaatgagg gtcttgactt taattgcgga tgaaaacctt tctaatacct caaatagcat
     9841 aaaaatagta tcttttatga caaaaaacac attatttagg gaaataaaaa ataattttgt
     9901 aatatttcta cataaaagtg tcaagaaacg gtaatattta aagggtatca tagaattata
     9961 gaaagaagga gaattttcaa atatgaaatc aacaactaaa aagattaaaa caactcttgc
    10021 aggagtagct gccttgtttg cagtatttgc tccatcattt gtatctgctc aagaatcatc
    10081 aacttacact gttaaagaag gtgatacact ttcagaaatc gctgaaactc acaacacaac
    10141 agttgaaaaa ttggcagaaa acaaccacat tgataacatt catttgattt atgttgatca
    10201 agagttggtt atcgatggcc ctgtagcgcc tgttgcaaca ccagcgccag ctacttatgc
    10261 ggcaccagcc gctcaagatg aaactgtttc agctccagta gtaagtgaaa cagttgtttc
    10321 aactgtaagc ggatctgaag cagaagccaa agaatggatc gctcaaaaag aatcaggtgg
    10381 tagctataca gctacaaatg gacgttatat cggacgttac caattaacag attcatacct
    10441 gaacggtgac tactcagctg aaaaccaaga acgtgtagca gatgcctacg ttgcaggacg
    10501 ttacggttca tggactgctg ctaaaaactt ctggcttaac aatggctggt attaagaact
    10561 aacgaacaaa taatatgtaa agagtcgagg aaatccctcg acttttttta taatttcttt
    10621 ggttgataat ggg
//
